METHODS FOR THE ANALYSIS OF BLOOD FACTORS FOR CALCULATING HEMOGLOBIN FROM OXYGEN BOUND BY 2 c.c. OF BLOOD Temperature Air dissolved by 2 c.c. of blood. Subtract from gas volume read in apparatus in order to obtain corrected gas volume, representing 62, set free from hemoglobin. FACTOR BY WHICH CORRECTED GAS VOLUME is MULTIPLIED IN ORDER TO GIVE : Oxygen chemically bound by 100 c.c. of blood. Per cent hemoglobin calculated on the basin: 18.5 per cent oxygen = 100 percent hemoglobin. C°. c.c. c.c. Per Cent 15 0.037 46.5x4 251 XT! 16 0.036 46.3x4 250X4 17 0.036 46.0x4 249X4 18 0.035 458xA 45.8X760 247x4 19 0.035 45.6X4 246X4 20 0.034 45.4x4 245X4 21 0.033 45.1x4 244X700 22 0.033 44.9x4 242X4 23 0.032 44.7x4 241X4 24 0.032 44.4x4 240X4 25 0.031 r> 44.2x4 239X4 26 0.030 44.0X4 o 237X760 27 0.030 43.7xA 236X4 28 0.029 43.5X4 235x4 29 0.029 43.3X4 234x4 30 0.028 43 ly^-«.ix760 233x4