PREFACE TO THE SECOND EDITION. IN preparing this second edition of u Pathogenic Bac- teria '' I have endeavored to bring the work up to date in all departments of the subject by introducing brief mention of all recent work accomplished in bacteriology. In order to aid the student whose particular interest might make him desire to refer to the original papers, I have thought well to depart from the plan of the orig- inal work and give the references in the form of foot- notes. Without departing too much from the primary descrip- tive purpose of the book, I have made it a special point to add considerably to the amount of technique it con- tains, and so make it fulfill the double purpose of a sys- tematic work upon bacteria and a laboratory guide. New chapters have been added dealing with the bac- teriology of Whooping-cough, Mumps, Yellow Fever, Hog-cholera, and Swine-plague ; describing the Bacillus aerogenes capsulatus and the Proteus vulgaris; and describing the Methods of Determining the Value of Antiseptics and Germicides, and of Determining the Thermal Death-point. To a number of friendly readers whose suggestions have been helpful in improving the work, I desire to extend my sincere thanks. JOSEPH