s A' '/'.•/ TAJA' AV A*\fIMsl/.S. or so and then separated. By this maneuver a subcuta- neous pocket is formed, into which the tissue is easily forced. The opening should not be large enough to re- quire subsequent stitching. vSinall animals, like rabbits and guinea-pigs, can be held in tlie hand, as a rule. Rabbit-holders of various forms can be obtained from dealers. Dogs, oats, sheep, and goats can be tied and held in I toughs, A convenient form of mouse-holder, invented by Kitasato, is shown in Pig. 42. In all these experiments one must remember that the amount of material introduced into the animal must be in proportion to its si/.e, and that injection-experiments upon mice generally are so crude and destructive as to warrant the comparison drawn by Frankel, that to inject a few minims of liquid into the pleura! cavity of a mouse is u much the same as if one would inject through a fire- hose three or four quarts of some liquid into the respira- tory organs of a man.11 The blood of animals, when it is necessary to experi- ment with it, is best secured from a large vein, generally the jugu- lar, From small animals, such as guinea-pigs, it may be secured by introducing a small eannula into the carotid artery. Our observations of animals by no means cease with their death, Indeed, he cannot be a bacteriol- ogist \vho is not already a good pathologist, and expert in the recog- nition of diseased organs. When an autopsy is to be made upon a small animal, it is best to wash it for a few moments in a disinfecting solution, to kill the germs present upon the hair and the skin, as well as to moisten the hair and enable it to be kept, out of the incision, ii