CHAPTER XI. THE BACTERIOLOGIC EXAMINATION OF THE AIR. IT has been repeatedly emphasized—and indeed at the present time almost every one knows—that micro-organ- isms float almost everywhere in the air, and that their presence there is a constant source of danger, not only of contamination in our bacteriologic researches, but also a menace to our health. Such micro-organisms are neither ubiquitous nor equally disseminated, but are much more numerous where the air is dusty than where it is pure—much more so where men and animals are accustomed to live, than upon the ocean or upon high mountain-tops. The purity of the atmo- sphere bears a distinct relation to the purity of the soil over which its currents blow. The micro-organisms that occur in the air are for the most part harmless saprophytes which have been sepa- rated from their nutrient birthplace and carried about by the wind. They are almost always taken up from dried materials, experiment having shown that they arise from the surfaces of liquids in which they grow with much dif- ficulty. They are by no means all bacteria, and a plate of sterile gelatin exposed for a brief time to the air will generally grow moulds and yeasts as well as bacteria. The bacteria present are occasionally pathogenic, espe- cially in localities where the discharges of diseased animals have been allowed to collect and dry. For this reason the atmosphere of the wards of hospitals and of rooms in which infectious cases are being treated is much more apt to contain them than the air of the street. However, the dried expectoration of cases of tuberculosis, of in- 164