INDEX. 487 Cladothrix, 39 » Closet, hot-air, 107 Clostridium, 34 Clothing, disinfection of, 120 Cocci, 36 morphology of, 36 Cohn's classification of the bac- teria, 41, 42 Comma bacillus, discovery of, 29 Cotton, sterile, value of, in bacte- riological work, 107 Cover-glass forceps, 93 preparations for general exam- ination, 91 Gram's method for staining, 99 method of fixing material for examination, 92 Cover-glasses, cleaning of, 92 Crystal-formation by bacillus pyo- cyaneus, 199 Cultivation of bacteria, 124 Culture-media, 124 agar-agar, 129 alkaline blood-serum, 133 blood agar-agar, 131 blood-serum, 131 bouillon, 124 free from dextrose, 137 Deycke's alkali-albuminate, 133 Eisner's, 371 Dunham's solution, 136 gelatin, 127 glycerin agar-agar, 131 hemoglobin agar-agar, 131 liquid, best means of keeping, 127 development of bacteria in, H7 litmus milk, 135 Loffler's blood-serum mixture, 133 milk, 135 peptone solution, 136 Petruschky's whey, 136 potato, 134 potato-juice, 135 sterilization of, 108 Cultures, anaerobic, various meth- ods for making, 153-157 by animal-inoculation, 148 plate-, 140 puncture-, 147 in gelatin, various appearances of, 149 "pure," 139 method of making, 146 solid or semi-solid, examination of bacteria in, 89 stroke-, 147 study of, 139 Culture-tubes, method of filling, 126, 127 method of inoculating, 141 Cumol for sterilization of catgut, 116 Czenzynke's staining-fluid, 447 DAVAINE'S classification of the bacteria, 41 Death-point, thermal, 176 Dejecta, sterilization of, 119 Deycke's alkali-albuminate, 133 Digestive tract, entrance of bac- teria into, 58 Diphtheria, antitoxic serum in treatment of, 302-305 determination of strength of, 303 preparation of, 302 preservation of, 302 antitoxin, 297 preparation of, 298 bacillus of, 284, 288, 289 discovery of, 29 relation of, to diphtheria, 292 susceptibility of different ani- mals to, 293 toxin elaborated by, 297 bacteriologic diagnosis of, 287 in man and in animals, 293, 294 pseudo-, bacillus of, 294 Diplococci, 36, 37 Diplococcus of mumps, 205 cultures of, 206 morphology of, 206