INDEX. 489 Filtration of toxins, apparatus for, 112 Fiocca's method of staining spores, 101 Fission, 33 Flagella, 31 staining of, 101 conditions essential to success in, 103 Pitfield's method, 103 Forceps, cover-glass, 93 Formaldehyde as a germicide, 114 in disinfection of rooms, 199, 122 regenerator, 115 Formalin, 114 in disinfection of rooms, 122 use of, as a disinfectant, 115 Fowl-tuberculosis, bacillus of, 238 Franke!'s instrument for obtaining earth from various depths for bacteriologic study, 174 method of making anaerobic cultures, 154 Friedlander's method of staining bacteria in tissue, 97 pneumonia bacillus, 352 cultures of, 353 pathogeny of, 354 Funnel for filling tubes with cul- ture-media, 126 Furniture, disinfection of, 121 GABBETT'S method of demonstrat- ing the presence of tuber- cle bacilli in sputum, 214 Cases, production of, by bacteria, 54 determination of, 54 Gelatin as a culture-medium, 127 growth of bacteria in, 148, 149 growth, microtome section of, 159, 160 liquefaction of, by bacteria, 53 Generation, spontaneous, doctrine of, 18 "Germ theory" of disease, 23 Germicidal value of g-aseous re- agents, determination of, 180 Germicidal value of reagents, deter- mination of, 178 Koch's method, 178 Stern berg's method, 179 Germicides, chemical action of, 117 value of different, 113, 144 Glanders, 248 bacillus of, 249 cause of, 248 injections of mallein in, 254 Glassware, sterilization of, 106 Glycerin agar-agar as a culture- medium, 131 -gelatin as an imbedding me- dium, 85 Gonococci, cultivation of, 202 Gonococcus, 201 in urethral pus, 201 Gonorrhea, 201 communication of, to animals, 203 Gram's method of staining bacteria in tissue, 97 of staining cover-glass prepa- rations, 99 solution for staining bacteria in tissue, 98 Gruber's method of making an- aerobic cultures, 154 HANDS, disinfection of, 115 Hanging-drop method of examin- ing Jiving micro-organisms, 88 Havelburg's bacillus, 405 Heat, moist, in sterilization of ap- paratus used in experi- mentation, 106 use of, in sterilization of instru- ments, etc., 106 Hemoglobin agar-agar, 131 Hesse's apparatus for collecting bacteria from the air, 165 method of making anaerobic cultures, 153 Heyroth's instrument for counting colonies of bacteria in Petri dishes, 170