r I

L*^

1 t., ri'1

To 10 ce of the nearly iu*u*r.L
the alcoholic calcium dilon ?e % ,
folded filter. Dilute tin «itr.*** * *• ' .
reaction with a neutral « Lit? - »' * , 1
acid, add citric acid or u:u m i ** j >
main portion of theeitrut«»s%ol it, *i». NJ
this process until a ntutrairtu 41-) »/,* t' '
water to make the specific xr u it v I I,M

Determination of Citrate-iaxsolutte
Heat 100 cc of ainnuniir > t t fcv •*,
flask placed in a water bat 'i a* t!. > t
stoppered to prevent ovaporutii \. I
bath. The level of the \vatt?r ri k! i
solution in the flask. \VLer thv ft ?
reached 65°, drop into it t?it f lt<c- i
in the determination of watt r-«« hJ> i>
2-gm sample of the oriidnal ,\ *t*'i7i r 'f *v ,., ,.*, »
be determined. Close the fkuA t ^ t,\ u,* , i ,
shake -violently until tht tilto- p.ptr . n :,-••,* t
minutes if no paper tuib 'H^II n-i I , « r L. i 4 - - ,
momentarily removing the stoppt r. 111,« +' « i*. .-
tain its contents at exactly 55°. >i in t . *'i^ «\» r
expiration of SOmmutesfrom thot nn t .r * t, T ^ i r ^
remove the flask from the hath aiu' in i r ,s »*, "v i, ti r *
as possible through quick-*ictin«, ffitt^ |up«r \\ i^*1
water at 55° until the volume tit th+> ^xtra^o ^ i >* **
for thorough draining each tinu vf»»rt idairA r *
Either (1) transfer the filter and *th u^ati^fv to A <-'i
organic matter is destroyed, add into 1.5 v of ..^ >t
acid and digest until all the phtnphutt +*> «LSM 'v* 4 k r
with contents to a digestion fljihl, t *id «i>»*• if 1*02^ivn*

VUl »^aflis LI

* .^ ^- t to
' r J . r ;$

n^h iTc* l>y

. At t at-

^rap.cily
v ,i*r time

til the

10 cc of concentrated hydrtvhlom icul
dissolved. There may be an insoluble rmiiiaM i v4 \\i
case. Ten minutes of digestion in tl»e vam» a* i J sL
dissolve all phosphates.

Dilute the solution as prepared in vi «>r - to 2<JO
through a dry filter and determine th< ph«» p
total phosphorus, pages 276 to 27^ dV'Lite t* i
insoluble phosphorus, as pento\id<\ dedret fn^i th
phosphorus p^ntoxide mid report tlu re IP under v* t
phosphoms pentoxide.

Non-acithdated Sam-pie*.— Treat "-! «^n ui tbt» nru
washing with water, as direcW for ae idulatc^l saiuplt's
contains much animal matter (aiifh n»iitt'ruil» as fisl a.
dissolve the residue ittsoluhle in annromuni intratt1 >n
(/>), (c) or (d)t page 27(5. Determine !*a direetttl for tot;il jtL«»p}'r.ru-*.

» gr-V un

- t a*i*% r th .

ititi iufi* J.eiii and

* t t'ie pb gphiite is
tei* "**^a *K eitHer
/"alJ b^ ftuffeient to

»*t Mix AC'!, filter

already d nct^i. ^or
^w^ »^nt *f citrate-
r D» r n^t «>f total
t of available

il \uthout previous
mikss tl-t- s jhstance
I ^orie m \v huh ease

f
I;