DAIRY PRODUCTS 227 ripening ferments. It contains, in the water free substance, not less than 50 per cent of fat. Manufacture.—The action of the enzyme rennin is to change the casein, by hydrolysis, into albumoses and peptone and soluble paracasein. The calcium salts present in solution in the* milk serum unite with the soluble paracasein to form an insoluble curd. The curd carries down not only the fat but also the greater part of the microorganisms contained in the milk. If these organisms are of a desirable kind they will produce a homo- geneous curd and good cheese, otherwise a spongy curd and a cheese of poor consistency will be the result. The table below gives the average composition of certain samples of four kinds of cheese, TABLE XXL—COMPOSITION OP CHEESE Primary Secondary Kind of cheese Water Fat Ash Proteins products of products of ripening ripening American "Cheddar" 34.2 33.7 3.8 25.2 . Roquefort . . . . 31.2 33 ?, 6.0 27.6 Swiss ........... 33.0 30 ?, 5 3 25.4 2.7 3.1 Gervais 44 8 36 7 2 9 12 7 1 9 0 8 Determination of Water.—Place about 15 gm of clean white sand in a flat aluminium dish, dry at 100° and weigh. Add about 3 gm of cheese and reweigh, quickly. More sand is needed if the cheese is very rich in fat. The mixture is then dried at 100° to constant weight and the per cent loss in weight calculated as moisture. Determination of Ash and Salt.—Ignite, cool and weigh a covered porce- lain crucible. Add about 15 gm of cheese, cover and reweigh. Uncover the crucible and drive off most of the moisture and volatile matter by carefully heating over a small flame. Place the uncovered crucible in a muffle furnace which is held at about 600° and through which air may circulate. Continue the heating until all carbon is burned. Cool in a desiccator and weigh. Calculate the per cent of ash. After weighing the ash, add water, dilute to 250 cc in a volumetric flask and mix. Either 50 or 100 cc of this solution is taken for the chlorine determination as directed on page 223. Calculate as sodium chloride. The residue insoluble in water may be dissolved in a small amount of dilute hydrochloric acid, diluted to 250 cc and calcium and phosphorus determined in aliquot parts of this solution, if desired. ij: li