FERTILIZERS 291 in 400 cc of water. Mix the solutions, dilute to 1000 cc and mix. To 100 cc of this solution add 65 cc of distilled water and 5 cc of glacial acetic acid, mix and allow to stand over night. This diluted solution is unstable and it should not be kept for use more than five days. Measure 17 cc of solution (6) into each tube, the temperature being not lower than 22°. Be sure that the graduated stems are filled, with entire absence of air bubbles. To one of the tubes add 5 cc of .___________, solution (a) and to each of the others 5 cc of the diluted sample. Whirl immediately for one minute at the rated speed for the centrifuge that is being used. Remove the tubes and tap those in which the upper surface of the column of precipitate is not practically plane. Whirl again for 15 seconds. The reading of the precipitate in the tubes containing the sample solutions should be within 5 divisions (either way) of that of the standard. If this is not the case, repeat the experiment, using more concentrated or more dilute solutions, as indicated. From the relative volumes of the precipitates and the known potassium content of the standard solution, calcu- late the per cent of potassium (or of potassium oxide) in the sample. Methods of Pot and Field Culture.—From analyses alone it is difficult to foretell just what will be the response of a plant to any given ap- plication of fertilizer to a soil. The great variety of soil components, including toxic substances often contained in them, is responsible for this, and it is very desirable that pot and field tests be conducted for the purpose of gaining more information as to the needs of the soil for the growth of any particular crop. This is analogous to conducting feeding experiments with animals for testing the degree of utilization and the physiological effects of the feeds. Much valuable information has been gained through sand and water culture experiments, in which solutions of certain com- pounds are added. Reference may be made to the experiments of Knop,1 Shives2 and Tottingham.3 lLandw. Vers. Sta., 7, 93 (1865). 2 N. J. Exp. Sta. Bull, 319 (1917). 3 J. Am. Soc. Agron., 2, 1 (1919). FIG. 60.— Graduated tube for determina- tion of potas- sium by the Sherrill centrif- ugal method.