MONTANA STATE LIBRARY 3 0864 0014 4419 2 REASSESSMENTS OF TRIBUTARIES OF THE BOULDER RIVER JEFFERSON COUNTY, MONTANA September, 1997 STATE DOCUMENTS COLLECTION r i MONTANA STATE LIBRARY 1515 E. 6th AVE. HEL-ENA, MONTANA 53G20 RECEIVED JUN 0 9 1999 DEQ / PPA jL-^wirHi* f< ^nt? M — vwit Bureau prepared for The Montana Department of Environmental Quality Helena, Montana by Wease Bollman Rhithron Biological Associates Missoula, Montana C May, 1999 XJl , i/\. :- '""3 ri!r..:.| -,$ I. iwr f£B 1 __ INTRODUCTION Benthic macro invertebrates are known to be important indicators of stream ecosystem health (Hynes 1970). Life spans for some of these creatures are as long as three or more years, and their complex life cycles and limited mobility mean that there is ample time and opportunity for the community to respond to cumulative effects of environmental perturbations. The analysis of macro invertebrate communities can thus be related to a stream's biological health, or integrity, defined by Karr and Dudley (1981) as ". . .the capability of supporting and maintaining a balanced, integrated, adaptive community of organisms having a species composition, diversity and functional organization comparable to that of natural habitat of the region." The multimetric approach to bioassessment using benthic macro invertebrates uses attributes of the assemblage in an integrated way to reflect overall biotic condition. Community attributes that can contribute meaningfully to bioassessment include assemblage structure, sensitivity of community members to stress or pollution, and functional traits. Each metric component contributes an independent measure of the biotic integrity of a stream site; combining components into an overall score reduces variance and increases precision of the assessment. (Fore et al. 1996). This report presents and interprets data collected in 1997 from tributaries of the Boulder River, using multimetric methodologies that are adaptations of the U.S. EPA's Rapid Bioassessment Protocols (RBP) (Plafkin et al. 1989). The streams have been exposed to varying degrees of impairment due to metal-mining impacts, and the study was undertaken to ascertain responses of the macro invertebrate communities to these impairments. METHODS Aquatic macro invertebrates were sampled by personnel of the Montana Department of Environmental Quality (MT DEQ) from five tributaries of the Boulder River in September of 1997. Sampling methods are described by Bukantis (1998) inMT DEQ's standard operating procedures for macro invertebrate sampling. Macro invertebrate samples were delivered to Rhithron Biological Associates for laboratory and data analyses. In the laboratory, the RBP III sorting method was used to obtain subsamples of 300 (+/- 10%) organisms from each sample when possible. Some samples contained fewer than 300 organisms; in these cases, entire sample contents were sorted and all organisms removed. Community structure, function, and sensitivity to impact were characterized for each subsample using two methods prescribed by MT DEQ (Bukantis 1998). First, data were evaluated using the Montane Ecoregions reference. In this approach, benthic communities were compared to reference criteria established by MT DEQ for streams of the Montane Ecoregions, defined by Omernik et al.(1997). The Montane Ecoregions reference approach uses seven metrics (Bukantis 1998). In the second approach, an internal reference was identified for the Boulder River tributaries from among the sites sampled. Based on the seven metric values, the Little Boulder River site provided the best internal reference, though Jack Creek above the Bullion mine was another alternative. Metric values for all sites were compared with values from the reference site, and scoring criteria followed the system devised by McGuire (1995). For each of these analyses, actual metric values were compared to the reference values to obtain metric scores. Bio integrity was estimated as the total bioassessment score, that is, the combined score for all metrics expressed as a percentage of the maximum possible score. Impairment classifications and "use support" designations were assigned, based on the total bioassessment score, according to criteria demonstrated in Table 3 (a and b). No habitat data or assessment accompanied the macro invertebrate samples, thus impairment due to water quality degradation, including that due to metals contamination could not be definitively distinguished from that due to habitat degradation. W RESULTS Macro invertebrate taxa lists, metric results and other information for each sample are given in the Appendix. The Montane Ecoregions reference Figure 1 compares total bioassessment scores from integrated and summed metrics using the ecoregion reference scoring criteria. Bukantis (1998) summarizes this method and criteria. Breakdown of scores for each metric is presented in Table 1( a,b,c and d). Based on scores compared to the ecoregion reference, Jack Creek appears to be unimpaired, or, at most, slightly impaired above the Bullion mine, but moderately impaired below the mine. Cataract Creek above Uncle Sam Gulch was non-to-slightly impaired, but was slightly impaired below the gulch. High Ore Creek was moderately impaired. Both Basin Creek above Basin and the Little Boulder River scored excellently for nearly all metrics, and were classified as unimpaired using these criteria. Jack Ck, Jack Ck. Basin Ck. above mine* below mine above Basin* Cataract Ck. Cataract Ck. High Ore Little above . above Ck. Boulder U.S.guich* . mouth* Rivet* Figure 1. Total bioassessment scores, based on the Montane Ecoregions reference, for tributaries of the Boulder River. Total scores are expressed as percent of maximum score. Scores for sites marked by asterisks are mean scores for replicated samples. The internal reference Figure 2 compares total bioassessment scores using an internal reference scoring criteria, with the Little Boulder River serving as the reference site. Table 2 summarizes the method and its scoring criteria. Table 4 (a,b,c and d) gives a breakdown of the scores of individual metrics for each site. C Jack Ck. Jack Ck. Basin Ck. Cataract Ck. Cataract Ck. High Ore Little above mine* below mine above above above Ck. Boulder Basin* U.S. gulch* mouth* River* Figure 2. Total bioassessment scores, based on the internal reference, for tributaries of the Boulder River. Total scores are expressed as percent of reference score. Scores for sites marked by asterisks are mean scores for replicated samples. The bioassessment approach using internal reference criteria compares the tributaries with each other rather than with streams throughout the Montane Ecoregions. In this context, Basin Creek compares much less favorably to the other sites than it did when ecoregion criteria were used; the total bioassessment score has fallen to 67% of that of the reference condition. All other sites scored similarly regardless of the reference criteria used. Macroinvertebrate communities Above the Bullion mine, Jack Creek exhibited a benthic community typical of a healthy montane stream. Eleven sensitive insect taxa were collected in the replicated samples; the most abundant taxon present was the stonefly Zapada columbiana. This shredder is relatively intolerant of habitat degradation and highly intolerant of metals contamination. Sample replicates taken at this site were highly variable in the total abundance of organisms present (67 organisms vs. approximately 361 organisms) and consequently in the calculated values of metrics. Total bioassessment scores (ecoregion reference) for the replicates differed by 29 percentage points, for a coefficient of variation (F)ofl7. 4.5 -i Jack Ck. Jack Ck. Basin Ck. Cataract Ck. Cataract Ck. High Ore Little above below mine above above above Ck. Boulder mine* Basin* U.S.gulch* mouth* River* Figure 3. Calculated values for the metals tolerance index for tributaries of the Boulder River. Sites marked by an asterisk are represented by mean scores from replicated samples. Higher values of the metals tolerance index indicate communities with an overall higher tolerance to metals pollution. Below the Bullion mine, a depauperate fauna strongly suggests severe impairment of water or habitat quality. The sample contained only 8 organisms. Figure 3 compares values for McGuixe's metals tolerance index (Bukantis 1998) for all sites. The macro invertebrate community collected from Jack Creek below the mine appears to be much more tolerant of metals contamination than the assemblage collected above the mine. The performance of the ecoregion reference and internal reference bioassessment methods- seem to underestimate the severity of impairment at this site. Replicate samples taken from Basin Creek above Basin produced similar total bioassessment scores using the ecoregion reference (V- 2.7). The mean metals tolerance index value of 3.18 is the second highest calculated among the sites studied, suggesting that metals may influence biotic health in Basin Creek. However, the relatively high total bioassessment score compared to the Montane Ecoregions reference suggests that biotic health is relatively unimpaired. Communities at this site were dominated by the stonefly Zapada cinctipes, and seven highly sensitive insect taxa were collected here. These included the mayflies Drunella doddsi and Epeorus grandis which were abundantly present in samples. These animals are both highly intolerant of a variety of anthropogenic impacts to water and habitat quality, including metals contamination. Above Uncle Sam Gulch, Cataract Creek communities yielded ten highly sensitive insect taxa, including the mayfly Caudatella hystrix and a predatory caddisfly in the Rhyacophila Vofixa Group {Rhyacophila iranda). Replicate samples were rather variable in calculated total bioassessment scores, however (V= 14.6), based upon the ecoregion criteria. Metals tolerance index values were low for Cataract Creek sites both above and below Uncle Sam Gulch, suggesting sensitivity to metals pollution. Communities at both locations were dominated by the sensitive heptageniid mayfly Eperous grandis. At the lower site, however, the samples produced evidence of defaunation; one replicate contained only 52 organisms, the other 98. As a result, total bioassessment scores, which classify this site as only slightly impaired, are suspect, since a depauperate fauna is strong evidence of severe impairment of water or habitat quality, unless scouring flow conditions have temporarily compromised the community. Both bioassessment methods seem to have overestimated biotic health at the Cataract Creek site below Uncle Sam Gulch. The sample collected at High Ore Creek contained only ten organisms, suggesting severe impairment of water and/or habitat quality. The metals tolerance index calculated for the community, 3.9, was the highest for any site in this study, suggesting that metals contamination may be a factor in limiting biotic health at this site. Again, bioassessment scores from either method seem to have overestimated biotic health at this site. Excellent total bioassessment scores with little variation between replicates (V= 5) were calculated for assemblages sampled from the Little Boulder River.. High taxa richness and EPT richness characterized the communities, which were dominated by the scraper Glossosoma sp. The metals tolerance index was calculated at 2.37, suggesting that metals contamination may have only a minimal effect on biotic health. CONCLUSIONS • Depauperate fauna at Jack Creek below the Bullion mine, at Cataract Creek below Uncle Sam Gulch, and at High Ore Creek suggests severe impairment of biotic health. Since habitat information was not available, the source of the impairment cannot be distinguished. However, the presence of metal mines near these locations strongly suggests that metals contamination may be a factor. Total bioassessment scores for these sites greatly underestimate the severity of impairment, especially at the lower Cataract Creek site. The underestimation may be due to the small sample size or to insensitivity of metrics. • Possible evidence of defaunation was also apparent at the upper Jack Creek site, above the Bullion mine, where one replicate contained relatively few organisms. The cause of the observed low abundance of benthos was not apparent from the community data. • A range of variation in total bioassessment scores (using ecoregion criteria) for individual sample replicates was noted. The coefficient of variation (V) for replicate pairs ranged from 17 (Jack Creek above the Bullion mine) to 2.7 (Basin Creek). Mean value for Ffor five replicate pairs was 8.7. • Basin Creek above Basin and the Little Boulder River had diverse, sensitive benthic communities comparable to those of unimpaired streams in the Montane Ecoregions. • Above Uncle Sam Gulch, Cataract Creek samples were too variable to give definitive results, however, the presence of abundant sensitive taxa suggests that the assemblage is unimpaired. ADDENDUM Results of a revised bioassessment method (Bollman 1998) for tributaries of the Boulder River are summarized in Figure 4. Table A tabulates coefficients of variation for each replicate pair based on results obtained from each of the three bioassessment methods used in this report. J Jack Ck. above Jack Ck. below Basin Ck. above Cataract Ck. Cataract Ck. High Ore Ck. Little Boulder mine* mine Basin* above above mouth* River* U.S.gulch* Figure 4. Total bioassessment scores for tributaries of the Boulder River, using a revised bioassessment method. Scores for sites marked by asterisks are mean scores for replicated samples. Table A. Coefficients of variation (V) for sample replicates when three bioassessment criteria are applied. Montane Ecoregions Internal reference Revised reference criteria criteria bioassessment method criteria Jack Creek above 17 10.5 3.1 Bullion mine Basin Creek above 2.7 7 0 Basin Cataract Creek 14.6 7 0 above U.S. Gulch Cataract Creek 4.2 7 8 below U.S. Gulch Little Boulder River 5 7.5 3.1 Mean V 8.7 7.8 2.8 c LITERATURE CITED Bollman, W. 1998. Improving~Stream Bioassessment Methods for the Montana Valleys and Foothill Prairies Ecoregion. Unpublished Master's Thesis. University of Montana. Bukantis, Bob. 1998. Rapid bioassessment macro invertebrate protocols: sampling and sample analysis SOP's. Montana Department of Environmental Quality. Water QuaKty Division. Working draft. Fore, L. S., J.R. Karr and R.W. Wisseman. 1996. Assessing invertebrate responses to human activities: evaluating alternative approaches. Jounal of the North American Benthological Society 15(2): 21 2-231. Hynes, HBN. 1970. The Ecology of Running Waters. University of Toronto Press. Karr, J.R and D.R Dudley. 1981. Ecological perspective on water quality goals. Environmental Management 1 1 :249-256. Plafkin, J.L., M.T. Barbour^ KID. Porter and S.K. Gross.. 1989. Rapid Bioassessment Protocols for Use in Streams and Rivers. Benthic Macro invertebrates and Fish. U.S. EPA. 444/4-89-001. J J c TABLES J f TABLES Table la. Metric values and bioassessments for Jack Creek, Boulder River drainage. September 5, 1997. Montane Ecoregions reference. Jack Creek Jack Creek Jack Creek above Bullion Mine above Bullion Mine below Bullion Mine metric R4J R4i2 R5.1 Taxa richness 19 33 6 EPT richness 13 21 2 Biotic index 1.27 1.33 3.00 % dominant taxon 30 22 38 % Collector (g+fl) 15 17 12.5 % Scrapers + Shredders 76 74 37 5 %EPT 87 89 38 metric score ————— ___ 0, 0, Taxa richness 1 3 ~q~ EPT richness 0 3 0 Biotic index 3 3 2 % dominant taxon 2 3 I % Collector (g+fT) 3 3 3 Vo Scrapers + Shredders 3 3 I %EPT 3 3 0 7 total score (max = 21) 15 21 % maximum 71 \qq classification * SL1 NON use support PARTIAL FULL PARTIAL * classifications: (NON) non-impaired, (SLI) slightly impaired, (MOD) moderately impaired, (SEV) severely impaired. . Table lb. Metric values and bioassessments for Basin Creek, Boulder River drainage. September 5, 1997. Montane Ecoregions reference. . metric Basin Creek above Basin R3.1 Basin Creek above Basin R.3.2 Taxa richness EPT richness Biotic index % dominant taxon % Collector (g+ff) % Scrapers + Shredders %EPT 33 19 2.91 18 40 51 73 37 20 2.92 24 37 54 78 metric score Taxa richness EPT richness Biotic index % dominant taxon % Collector (g+ff) % Scrapers + Shredders %EPT 3 2 3 3 3 2 3 3 3 3 3 3 2 3 total score (max = 21) % maximum classification * use support 19 90 NON FULL 20 95 NON FULL * classifications: (NON) non-impaired, (SL1) slightly impaired, (MOD) moderately impaired, (SUV) severely impaire j I Table lc. Metric values and bioassessments for Cataract Creek, Boulder River drainage. September 5, 1997. Montane Ecoregions reference. metric Cataract Creek above Uncle Sam Gulch R2.1 Cataract Creek above Uncle Sam Gulch R2.2 Cataract Creek below Uncle Sam Gulch Rl.l Cataract Creek below Uncle Sam Gulch R1.2 Taxa richness EPT richness Biotic index % dominant taxon % Collector (g+ff) % Scrapers + Shredders %EPT 31 16 2.04 36 31 59 70 35 23 2.06 13 43 36 74 11 6 1.12 56 6 85 79 17 9 1.16 43 II 69 83 metric score Taxa richness EPT richness Biotic index % dominant taxon % Collector (g+ff) % Scrapers + Shredders %EPT 3 1 3 1 3 3 2 0 0 3 0 3 3 3 total score (max % maximum classification * use support 2!) 14 67 SLI PARTIAL 19 90 NON FULL 12 57 SLI PARTIAL 13 62 SLI PARTIAL * classifications: (NON) non-impaired, (SLI) slightly impaired, (MOD) moderately impaired, (SEV) severely impaired. 10 Table Id. Metric values and bioassessments for High Ore Creek and the Little Boulder River, Boulder River drainage. September 12, 1997. Montane Ecoregions reference. High Ore Creek Little Boulder River Little Boulder River metric RJ RJJ R 1.2 Taxa richness 6 38 39 EPT richness 2 21 23 Biotic index 3.00 2.09 1.72 % dominant taxon 40 43 22 % Collector (g+fr) 20 31 26 % Scrapers + Shredders 0 62 69 %EPT 50 75 81 metric score Taxa richness 0 3 3 EPT richness 0 3 3 Biotic index 2 3 3 % dominant taxon I 1 3 % Collector (g+flf) 3 3 3 % Scrapers + Shredders 0 3 3 %EPT ^ 1 3 3 total score (max = 21) 7 19 21 % maximum 33 90 100 classification* MOD NON NON use support PARTIAL FULL FULL * classifications: (NON) non-impaired. (SLI) slightly impaired, (MOD) moderately impaired, (SBV) severely impaired. C 80% 80-60% 60-40% < 40% a EPT richness 23 >85% 85-70% 70-50% < 50% a Biotic index 1 >72 >90% 90-80% 80-70% < 70% b % dominant 9? >60% 60-45% 45-30% < 30% b taxon % Gath. + Filt. 26 >90% 90-80% 80-70% < 70% b % Scraper 69 >80% 80-60% 60-40% < 40% a +Shredder %EPT 81 >75% 75-50% 50-25% < 25% a * a = score is ratio of study site to reference x 100. * b = score is ratio of reference to study site x 100. Table 3a. Criteria for the assignment of support classifications / standards violation thresholds (from Bukantis, 1997) % Comparability to reference Use support >75 Full support— standards not violated 25-75 Partial support—moderate impairment-standards violated <25 Non-support— severe impairment- -standards violated Table 3b. Criteria for the assignment of impairment classifications (from Plafkin et al. 1989). % Comparability to reference Classification >83 nonimpaired 54-79 slightly impaired 21-50 moderately impaired <17 severely impaired 12 Table 4a. Percentage of internal reference for metrics, and bioassessments for Jack Creek, Boulder River drainage. September 5, 1997. Jack Creek Jack Creek Jack Creek above Bullion Mine above Bullion Mine below Bullion Mine metric R4.1 R 4.2 R_5J Taxa richness 49 85 15 EPT richness 57 91 9 Biotic index 100 100 57 % dominant taxon 73 100 58 % Collector (g+ff) 100 100 100 % Scrapers + Shredders 100 100 54 %EPT 100 100 47 metric score Taxa richness 1 3 0 EPT richness 1 3 0 Biotic index 3 3 0 % dominant taxon 3 3 2 % Collector (g+ff) 3 3 3 % Scrapers + Shredders 3 3 1 %EPT 3 3 1 total score (max = 21) 17 21 7 % reference 81 100 33 classification * SL1 NON MOD use support FULL FULL PARTIAL * classifications: (NON) non-impaired, (SL1) slightly impaired, (MOD) moderately impaired, (SliV) severely impaired. . 13 C C I Table 4b. Percentage of internal reference for metrics and bioassessments for Basin Creek, Boulder River drainage. September 5, 1997. metric Basin Creek above Basin R3.1 Basin Creek above Basin R3.2 Taxa richness EPT richness Biotic index % dominant taxon % Collector (g+ff) % Scrapers + Shredders %EPT 85 83 59 100 65 74 90 95 87 59 92 70 78 96 metric score Taxa richness EPT richness Biotic index % dominant taxon % Collector (g+ff) % Scrapers + Shredders %EPT 3 2 0 3 0 2 3 3 3 0 3 1 2 3 total score (max ; % reference classification * use support 21) 13 62 SLI PARTIAL 15 71 SLI PARTIAL * classifications: (NON) non-impaired, (SLI) slightly impaired, (MOD) moderately impaired, (SRV) severely impaire 14 Table 4c. Metric values, percentage of reference and bioassessments for Cataract Creek, Boulder River drainage. September 5, 1997. metric Cataract Creek above Uncle Sam Gulch R2.1 Cataract Creek above Uncle Sam Gulch R2.2 Cataract Creek below Uncle Sam Gulch R 1.1 Cataract Creek below Uncle Sam Gulch R 1.2 Taxa richness EPT richness Biotic index % dominant taxon % Collector (g+ff) % Scrapers + Shredders %EPT 79 70 84 61 84 86 86 90 100 83 100 60 52 91 28 26 100 39 100 100 98 44 39 100 51 100 100 100 metric score Taxa richness EPT richness Biotic index % dominant taxon % Collector (g+ff) % Scrapers + Shredders %EPT 3 3 2 3 0 1 3 total score (max : % reference classification * use support 21) 17 81 SLI FULL 15 71 SLI PARTIAL 13 62 SLI PARTIAL 15 71 SLI PARTIAL * classifications: (NON) non-impaired, (SLI) slightly impaired, (MOD) moderately impaired, (SEV) severely impaired. 15 c c f Table 4d. Metric values, percentage of reference and bioassessments for High Ore Creek and the Little Boulder River, Boulder River drainage. September 12, 1997. High Ore Creek Little Boulder River Little Boulder River metric RJ R 1.1 R 1.2 Taxa richness 15 97 1 00 EPT richness 9 91 100 Biotic index 51 82 100 % dominant taxon 55 51 100 % Collector (g+ff) 100 84 100 % Scrapers + Shredders 0 90 100 %EPT 62 93 100 metric score Taxa richness 0 3 3 EPT richness 0 3 3 Biotic index 0 2 3 % dominant taxon 2 2 3 % Collector (g+ff) 3 2 3 % Scrapers + Shredders 0 3 3 %EPT 2 3 3 total score (max = 21) 7 18 21 % reference 33 86 100 classification * MOD NON NON use support PARTIAL FULL FULL * classifications: (NON) non-impaired, (SLI) slightly impaired, (MOD) moderately impaired, (SEV) severely impaired. . 16 Table 4d. Metric values, percentage of reference and bioassessments for High Ore Creek and the Little Boulder River, Boulder River drainage. September 12, 1997. High Ore Creek Little Boulder River Little Boulder River metric RJ RJJ R 1,2 Taxa richness 15 97 100 EPT richness 9 91 100 Biotic index 51 82 100 % dominant taxon 55 51 100 % Collector (g+ff) 100 84 100 % Scrapers + Shredders 0 90 100 %EPT 62 93 100 metric score Taxa richness 0 3 3 EPT richness 0 3 3 Biotic index 0 2 3 % dominant taxon 2 2 3 % Collector (g+ff) 3 2 3 % Scrapers + Shredders 0 3 3 %EPT 2 3 3 total score (max = 21) 7 18 21 % reference 33 86 100 classification* MOD NON NON use support PARTIAL FULL FULL * classifications: (NON) non-impaired, (SL1) slightly impaired, (MOD) moderately impaired, (SHV) severely impaired, . c c 16 L , APPENDIX Macroinvertebrate Taxonomic Data Jack Ck. above BoulJion Mine 970905 R4. 1 Taxon % BI FFG Oligochaeta: Enchytraeidae TOTAL: MISC. TAXA Baetis tricaudatus Epeorus albertae Epeorus grandis Rhithrogena robusta Ameletus TOTAL: EPHEMEROPTERA Visoka cataractae Zapada columbiana Doroneuria Megarcys Yoraperla TOTAL: PLECOPTERA Arctopsyche grandis Glossosoma Rhyacophila Sibirica Gr. TOTAL: TRICHOPTERA Heterlimnius TOTAL: COLEOPTERA Chelifera Hexatoma TOTAL: DIPTERA Cricotopus Nostococladius P arametriocnem us TOTAL: CHIRONOMIDAE GRAND TOTAL 2 2.99 2 2.99 1 1.49 2 2.99 8 11.94 20 29.85 1 1.49 32 47.76 3 4.48 12 17.91 1 1.49 2 2.99 2 2.99 20 29.85 2 2.99 3 4.48 1 1.49 6 8.96 3 4.48 3 4.48 1 1.49 1 1.49 2 2.99 1 1.49 1 1.49 2 2.99 67 100.00 10 CG 4 CG 2 sc 0 . sc 0 sc 0 CG 0 SH 2 SH 0 PR 1 PR 0 SH 2 CF 0 SC 0 PR CG 5 PR 2 PR 6 SH 5 CG A-l Macroinvertebrate Taxonomic Data Jack Creek above Boullion Mine 970905 R4.2 Taxon % Bl FFG Oligochaeta: Enchytraeidae Acari TOTAL: MISC. TAXA Baetis tricaudatus Drunella doddsi Cinygmula Epeorus albertae Epeorus grandis Rhithrogena robusta Ameletus TOTAL: EPHEMEROPTERA Visoka cataractae Zapada columbiana Doroneuria Megarcys Setvena bradleyi Yoraperla TOTAL: PLECOPTERA Parapsyche elsis Glossosoma Worraaldia Rhyacophila Betteni Gr. Rhyacophila Brunnea Gr. Rhyacophila Hyalinata Gr. Rhyacophila Vorixa Gr. Neothremma alicia TOTAL: TRICHOPTERA Hetedimnius TOTAL: COLEOPTERA Prosimulium Dicranota Hexatoma TOTAL: DIPTERA Brillia Cricotopus Nostococladius Eukiefferiella Brehmi Gp. Micropsectra Pagastia Rheocricotopus TOTAL: CHIRONOM1DAE GRAND TOTAL 13 4.23 1 0.33 14 4.56 8 2.61 1 0.33 6 1.95 9 2.93 68 22.15 46 14.98 2 0.65 140 45.60 10 3.26 6* 22.48 3 0.98 5 1.63 4 1.30 9 2.93 100 32.57 13 4.23 3 0.98 2 0.65 2 0.65 2 0.65 6 1.95 4 1.30 1 0.33 33 10.75 7 2.28 7 2.28 2 0.65 1 0.33 1 0.33 4 1.30 3 0.98 2 0.65 1 0.33 1 0.33 1 0.33 1 0.33 9 2.93 307 100.00 10 CG 5 PA 4 CG 1 SC 0 sc 2 SC 0 sc 0 sc 0 CG 0 SH 2 SH 0 PR 1 PR 0 PR 0 SH 0 CF 0 SC 0 CF 0 PR 2 PR 0 PR 0 PR 1 SC CG 4 CF 3 PR 2 PR 4 SH 6 SH 8 CG 4 CG 1 CG 4 CG Aquatic Macroinvertebrate Data: Jack Creek above Bullion mine: September 5, 1997 Sample: 4A 4.2 % of sample used: 100 85 Subsample size 67 307 Taxa richness 19 33 EPT richness 13 21 Biotic index 1.27 1.33 % Dominant taxon 30 22.5 % EPT 87 89 % Collectors (g+f) 15 17 % Scrapers + Shredders 76 74 % Hydropsychinae of Trich 0 0 Metals tolerance index 1.51 1.12 Shannon Diversity (log2) 3.41 3.69 EPT/Chironomidae 29 30 CTQa . 47.79 49.45 %Baetidae of Ephemeroptera 3 6 % Coleoptera 4.5 2 % Diptera 3 1 % Chironomidae 3 3 % Ephemeroptera 48 46 % Plecoptera 30 33 %Trichoptera 9 11 % multivoltine 3 4 % univoltine 85 84 % semivoltine 11 12 Functional Feeding Grp. %RA # taxa %RA # taxa Filterers 3 1 6 3 Collector-Gatherers 12 5 11 8 Shredders 27 4 30 5 Scrapers 49 4 44 7 Predators 9 5 9 9 Est. total number of organisms 67 361 Est. number collected per foot 4.5 18 Est. number collected per minute 67 361 A-3 ^ Macroinvertebrate Taxonomic Data Jack Creek below Boullion Mine 970905 R5.1 Taxon % BI FFG Rhyacophila Hyalinata Gr. Rhyacophila Vofixa Gr. TOTAL: TRICHOPTERA Petrophila TOTAL: LEPIDOPTERA Heterlimnius TOTAL: COLEOPTERA Atherix TOTAL: DIPTERA Brillia TOTAL: CHIRONOMIDAE GRAND TOTAL 1 12.50 2 25.00 3 37.50 1 12.50 1 12.50 1 12.50 1 12.50 1 12.50 1 12.50 2 25.00 2 25.00 8 100.00 1 1 PR PR 5 SC 3 CG .5 PR 4 SH A-4 Aquatic Macroinvertebrate Data: Jack Creek below Bullion mine: September 5, 1997 Sample: 5J % of sample used: 1 00 Subsample size 8 Taxa richness 6 EPT richness 2 Biotic index 3.00 % Dominant taxon 25 %EPT 38 % Collectors (g+fj 12.5 % Scrapers + Shredders 37.5 % Hydropsychinae of Trich 0 Metals tolerance index 2.75 Shannon Diversity (log2) 2. 1 6 EPT/Chironomidae 1 . 5 CTQa undefined %Baetidae of Ephemeroptera 0 % Coleoptera 12.5 %Diptera 12.5 % Chironomidae 25 % Ephemeroptera 0 % Plecoptera 0 % Trichoptera 37.5 % multivoltine 1 9 % univoltine 50 % semivoltine 3 1 Functional Feeding Grp. %RA # taxa Filterers Collector-Gatherers Shredders Scrapers Predators Est. total number of organisms Est. number collected per foot Est. number collected per minute 0 0 12.5 1 25 1 12.5 1 50 8 <1 8 3 A-5 ^ Macroinvertebrate Taxonomic Data Basin Creek above Basin 970905 R3.1 Taxon $_ _J^__ BI FFG Nematoda Oligochaeta: Enchytraeidae Acari TOTAL: MISC. TAXA Baetis tricaudatus Drunella doddsi Cinygmula Epeorus longimanus Epeorus grandis Rhithrogena robusta TOTAL: EPHEMEROPTERA Paraperla Zapada cinctipes Doroneuria Hesperoperla pacifica Megarcys TOTAL: PLECOPTERA Arctopsyche grandis Brachycentrus americanus Micrasema Glossosoma Hydropsyche Lepidostoma Rhyacophila Brunnea Gr. Rhyacophila Hyalinata Gr. TOTAL: TRICHOPTERA Heterlimnius Narpus Optioservus Zaitzevia TOTAL: COLEOPTERA Ceratopogonidae Simulium Rhabdomastix TOTAL: DIPTERA Cricotopus Eukiefferiella Devonica Gr. Micropsectra Rheocricotopus TOTAL: CHIRONOMIDAE GRAND TOTAL 2 0.63 4 1.27 ■> j 0.95 9 2.85 24 7.59 15 4.75 1 0.32 1 0.32 17 5.38 10 3.16 68 21.52 1 0.32 56 17.72 8 2.53 2 0.63 6 1.90 73 23.10 31 9.81 4 1.27 2 0.63 34 10.76 16 5.06 1 0.32 1 0.32 1 0.32 90 28.48 2 0.63 5 1.58 14 4.43 9 2.85 30 9.49 4 1.27 5 1.58 2 0.63 11 3.48 31 9.81 2 0.63 1 0.32 1 0.32 35 11.08 316 100.00 5 OM 10 CG 5 PA 4 CG 1 SC 0 sc 1 SC 0 sc 0 sc 0 PR 3 SH 0 PR 1 PR 1 PR 2 CF 1 SC 1 SH 0 SC 5 CF 1 SH 2 PR 0 PR 3 CG 2 SH 5 SC 4 CG 6 PR 5 CF 1 PR 7 CG 8 CG 4 CG 4 CG Macroinvertebrate Taxonomic Data Basin Creek above Basin 970905 R3.1 J Taxon Nematoda Oligochaeta: Enchytraeidae Acari TOTAL: MISC. TAXA Baetis tricaudatus Drunella doddsi Cinygmula Epeorus longimanus Epeorus grandis Rhithrogena robusta TOTAL: EPHEMEROPTERA Paraperla Zapada cinctipes Doroneuria Hesperoperla pacifica Megarcys TOTAL: PLECOPTERA Arctopsyche grandis Brachycentrus americanus Micrasema Glossosoma Hydropsyche Lepidostoma Rhyacophila Brunnea Gr. Rhyacophila Hyalinata Gr. TOTAL: TRICHOPTERA Heterlimnius Narpus Optioservus Zaitzevia TOTAL: COLEOPTERA Ceratopogonidae Simulium Rhabdomastix TOTAL: DIPTERA Cricotopus Eukiefferiella Devonica Gr. Micropsectra Rheocricotopus TOTAL: CHIRONOMIDAE GRAND TOTAL % ? 0.63 4 1.27 3 0.95 9 2.85 24 7.59 15 4.75 1 0.32 1 0.32 17 5.38 10 3.16 68 21.52 1 0.32 56 17.72 8 2.53 2 0.63 6 1.90 73 23.10 31 9.81 4 1.27 2 0.63 34 10.76 16 5.06 1 0.32 1 0.32 1 0.32 90 28.48 2 0.63 5 1.58 14 4.43 9 2.85 30 9.49 4 1.27 5 1.58 2 0.63 11 3.48 31 9.81 2 0.63 1 0.32 1 0.32 35 11.08 316 100.00 BI FFG 5 OM 10 CG 5 PA 4 CG 1 sc 0 sc 1 sc 0 sc 0 sc 0 PR 3 SH 0 PR 1 PR 1 PR 2 CF 1 SC 1 SH 0 SC 5 CF 1 SH 2 PR 0 PR 3 CG 2 SH 5 SC 4 CG 6 PR 5 CF 1 PR 7 CG 8 CG 4 CG 4 CG ^ Macroinvertebrate Taxonomic Data Basin Creek above Basin 970905 R3. 2 Taxon % BI FFG Turbellaria Nematoda Oligochaeta: Enchytraeidae Acari TOTAL: MISC. TAXA Baetis tricaudatus Drunella doddsi Cinygmula Epeorus grandis Rhithrogena robusta TOTAL: EPHEMEROPTERA Leuctridae Amphinemura Zapada cinctipes Doroneuria Hesperoperla pacifica Megarcys Skwala Pteronarcella TOTAL: PLECOPTERA Arctopsyche grandis Brachycentrus americanus Micrasema Glossosoma Hydropsyche Lepidostoma Rhyacophila Brunnea Gr. TOTAL: TRICHOPTERA Heterlimnius Narpus Optioservus TOTAL: COLEOPTERA Atherix Simulium Antocha Rhabdomastix TOTAL: DIPTERA Brillia Diamesa Eukiefferiella Devonica Gr. Micropsectra Orthocladius Rheocricotopus TOTAL: CHIRONOMIDAE GRAND TOTAL 1 0.30 2 0.60 11 3.29 1 0.30 15 4.49 35 10.48 21 6.29 3 0.90 17 5.09 7 2.10 83 24.85 1 0.30 2 0.60 SO 23.95 5 1.50 1 0.30 12 3.59 1 0.30 1 0.30 103 30.84 22 6.59 6 1.80 2 0.60 19 5.69 17 5.09 4 1.20 2 0.60 72 21.56 3 0.90 7 2.10 10 2.99 20 5.99 1 0.30 5 1.50 1 0.30 2 0.60 9 2.69 1 0.30 4 1.20 ! 0.30 1 0.30 19 6.89 2 0.60 32 9.58 330 100.00 4 PR 5 OM 10 CG 5 PA 4 CG 1 SC 0 SC 0 SC 0 SC 0 SH 2 SH 3 SH 0 PR 1 PR 1 PR 5 PR 4 SH 2 CF 1 SC 1 SH 0 SC 5 CF 1 SH 2 PR 3 CG 2 SH 5 SC 5 PR 5 CF 3 CG 1 PR 4 SH 5 CG 8 CG 4 CG 6 CG 4 CG A -7 Aquatic Macroinvertebrate Data: Basin Creek above Basin, September 5, 1997 Sample: 3.1 3.2 % of sample used: ~" ~~ 100 100 Subsample size 316 330 Taxa richness 33 37 EPT richness 19 20 Biotic index 2.91 2.93 % Dominant taxon 18. 24 % EPT 73 78 % Collectors (g+f) 40 37 % Scrapers + Shredders 5 1 54 % Hydropsychinae ofTrich 18 24 Metals tolerance index 3.43 2.93 Shannon Diversity (log2) 4.11 4.11 EPT/Chironomidae 6.6 9.2 CTQa 59.76 57.70 %Baetidae of Ephemeroptera 35 42 % Coleoptera 9 6 % Diptera 3 3 % Chironomidae 11 10 % Ephemeroptera 22 25 %Plecoptera 23 31 % Trichoptera 28 22 % multivoltine 17 17 % univoltine 58 65 % semivoltine 25 19 Functional Feeding Grp. %RA # taxa %RA # taxa Filterers 16 3 13 3 Collector-Gatherers 23 8 24 9 Shredders 20 4 29 8 Scrapers 30 8 25 7 Predators 8 8 7 8 Est. total number of organisms 316 330 Est. number collected per foot 18 16.5 Est. number collected per minute 316 330 A-8 J w Macroinvertebrate Taxonomic Data Cataract Ck above US Gulch 970905 R2.1 L Taxon # % BI FFG Nematoda 1 0.30 Oligochaeta: Enchytraeidae 23 6.99 TOTAL: MISC. TAXA 24 7.29 Baetis bicaudatus 4 1 .22 Baetis tricaudatus 3 0.91 Caudatella heterocaudata 2 0.61 Drunella doddsi 8 2.43 Epeorus longimanus 3 0.91 Epeorus grandis 119 36.17 Rhithrogena robusta 6 1.82 TOTAL: EPHEMEROPTERA 145 44.07 Zapada cinctipes 6 1 .82 Zapada columbiana 7 2.13 Doroneuria 6 1.82 Megarcys 13 3.95 TOTAL: PLECOPTERA 32 9.73 Micrasema 15 4.56 Glossosoma 27 8.21 Rhyacophila Betteni Gr. 3 0.91 Rhyacophila Brunnea Gr. 6 1.82 Rhyacophila Vofixa Gr. 2 0.61 TOTAL: TRICHOPTERA 53 16.11 Cleptelmis 7 2.13 Heterlimnius 18 5.47 TOTAL: COLEOPTERA 25 7.60 Simulium 1 0.30 Antocha 1 0.30 Dicranota 1 0.30 Hexatoma 1 0.30 TOTAL: DIPTERA 4 1.22 Brillia 2 0.61 Eukiefferiella Brehmi Gr. 14 4.26 Eukiefferiella Devonica Gr. 3 0.91 Eukiefferiella Gracei Gr. 1 0.30 Micropsectra 2 0.61 Orthocladius 22 6.69 Pagastia 1 0.30 Tvetenia Bavarica Gr. 1 0.30 TOTAL: CHIRONOMIDAE 46 13.98 GRAND TOTAL 329 100.00 5 OM 10 CG 2 CG 4 CG 0 SC 1 SC 1 SC 0 SC 0 SC 3 SH 2 SH 0 PR 1 PR 1 SH 0 SC 0 PR 2 PR 0 PR 4 CG 3 CG 5 CF 3 CG 3 PR 2 PR 4 SH 4 CG 8 CG 4 CG 4 CG 6 CG 1 CG 5 CG A-? Macroinvertebrate Taxonomic Data Cataract Ck. above Uncle Sam gulch 970905 R2.2 J Taxon % BI FFG Turbellaria Nematoda Oligochaeta: Enchytraeidae Acari TOTAL: MISC. TAXA Acentrella insignificans Baetis bicaudatus Baetis tricaudatus Caudatella hystrix Drunella doddsi Epeorus longimanus Epeorus grandis Rhithrogena Ameletus TOTAL: EPHEMEROPTERA Chloroperlidae Zapada cinctipes Zapada columbiana Doroneuria Megarcys Yoraperla TOTAL: PLECOPTERA Arctopsyche grandis Micrasema Glossosoma Apatania Rhyacophila Betteni Gr. Rhyacophila Brunnea Gr. Rhyacophila narvae Rhyacophila Vofixa Gr. TOTAL: TRICHOPTERA Cleptelmis Heterlimnius TOTAL: COLEOPTERA Limnophila TOTAL: DIPTERA Brillia Cricotopus EukierTeriella Devonica Gr. Eukiefferiella Brehmi Gr. Orthocladius TOTAL: CHIRONOMIDAE GRAND TOTAL 1 0.36 1 0.36 11 3.94 1 0.36 14 5.02 1 0.36 9 3.23 7 2.51 5 1.79 6 2.15 3 1.08 37 13.26 8 2.87 26 9.32 102 36.56 1 0.36 15 5.38 6 2.15 9 3.23 27 9.68 1 0.36 59 21.15 4 1.43 6 2.15 14 5.02 1 0.36 3 1.08 15 5.38 1 0.36 1 0.36 45 16.13 9 3.23 21 7.53 30 10.75 1 0.36 1 0.36 2 0.72 1 0.36 1 0.36 4 1.43 20 7.17 28 10.04 279 100.00 4 PR 5 OM 10 CG 5 PA 4 CG 2 CG 4 CG 0 CG 1 sc 1 sc 0 sc 0 sc 0 CG 1 PR 3 SH 2 SH 0 PR 1 PR 0 SH 2 CF 1 SH 0 SC 3 SC 0 PR 2 PR 0 PR 0 PR 4 CG 3 CG MH 4 SH 7 CG 8 CG 4 CG 6 CG \J A-iO Aquatic Macroinvertebrate Data: Cataract Creek above Uncle Sam Gulch, September 5, 1997. Sample: 2.1 2.2 % of sample used: 85 100 Subsample size 329 279 Taxa richness 32 35 EPT richness 1 6 23 Biotic index 2.04 2.06 % Dominant taxon 36 13 % EPT 70 74 % Collectors (g+f) 31 43 % Scrapers + Shredders 59 36 % Hydropsychinae of Trich 0 0 Metals tolerance index 1.82 2.05 Shannon Diversity (log2) 3.66 4.38 EPT/Chironomidae 5 7 CTQa 59.84 54.20 %Baetidae of Ephemeroptera 5 17 % Coleoptera 8 11 % Diptera 1 <1 % Chironomidae 14 10 % Ephemeroptera 44 37 % Plecoptera 10 21 % Trichoptera 16 16 % multivoltine 12 13 % univoltine 73 66 % semivoltine 15 21 Functional Feeding Grp. %RA # taxa %RA # taxa Filterers .3 1 1 1 Collector-Gatherers 30 13 41 12 Shredders 9 4 11 5 Scrapers 50 6 25 6 Predators 10 7 21 8 Est. total number of organisms 387 279 Est. number collected per foot 22 36 Est. number collected per minute 363 279 A-ll Macroinvertebrate Taxonomic Data Cataract Creek above mouth 970905 Rl . 1 Taxon BI FFG Epeorus longimanus Epeorus grandis TOTAL: EPHEMEROPTERA Chloroperlidae Amphineraura Megarcys TOTAL: PLECOPTERA Brachycentrus americanus TOTAL: TRICHOPTERA Rhabdomastix TOTAL: DIPTERA Brillia Cricotopus Trifascia Gr. Orthocladius Rheocricotopus TOTAL: CHIRONOMIDAE GRAND TOTAL 1 1.92 29 55.77 30 57.69 1 1.92 1 1.92 3 5.77 5 9.62 6 11.54 6 11.54 1 1.92 1 1.92 7 13.46 1 1.92 1 1.92 1 1.92 10 19.23 52 100.00 1 SC 0 sc 1 PR 2 SH 1 PR 1 SC 1 PR 4 SH 6 CG 6 CG 4 CG J A-iZ Macroinvertebrate Taxonomic Data Cataract Ck nr mouth 970905 Rl .2 Taxon % BI FFG Nematoda TOTAL: MISC. TAXA . Drunella doddsi Epeorus grandis TOTAL: EPHEMEROPTERA Chloroperlidae (immature) Sweltsa Hesperoperla pacifica Megarcys TOTAL: PLECOPTERA Arctopsyche grandis Brachycentrus americanus Hydropsyche Limnephilidae (immature) TOTAL: TRICHOPTERA Heterlimnius Zaitzevia TOTAL: COLEOPTERA Ceratopogonidae TOTAL: DIPTERA Brillia Orthocladius Pagastia Tvetenia Bavarica Gr. TOTAL: CHIRONOMIDAE GRAND TOTAL 1 1.02 1 1.02 4 4.08 42 42.86 46 46.94 8 8.16 1 1.02 1 1.02 6 6.12 16 16.33 1 1.02 16 16.33 1 1.02 1 1.02 19 19.39 3 3.06 1 1.02 4 4.08 2 2.04 2 2.04 5 5.10 2 2.04 2 2.04 1 1.02 10 10.20 98 100.00 OM 1 SC 0 sc 1 PR 0 PR 1 PR 1 PR 2 CF 1 SC 5 CF 3 SH 3 CG 4 CG PR 4 SH 6 CG 1 CG 5 CG c A-13 Aquatic Macroinvertebrate Data: Cataract Creek below Uncle Sam Gulch, September 5, 1997. Sample: U 1-2 % of sample used: 100 100 Subsample size 52 98 Taxa richness 11 18 EPT richness 6 1 0 Biotic index 1.12 1.16 % Dominant taxon 56 43 % EPT 79 83 % Collectors (g+f) 6 1 1 % Scrapers + Shredders 85 69 % Hydropsychinae of Trich 0 5 Metals to lerance index 1-50 1 . 84 Shannon Diversity (log2) 2.22 2.94 EPT/Chironomidae 4.1 8 CTQa 56.73 68.28 %Baetidae of Ephemeroptera 0 0 % Coleoptera 0 4 % Diptera 2 2 % Chironomidae 19 10 % Ephemeroptera 58 47 %Plecoptera 10 16 %Trichoptera 12 19 % multivoltine 14 9 %univoltine 74 69 % semivoltine 12 22 W Functional Feeding Grp. %RA #taxa %RA #taxa Filterers 0 0 2 2 Collector-Gatherers 6 3 9 5 Shredders 15 2 6 2 Scrapers .69 3 63 3 Predators 10 3 18 5 Est. total number of organisms 52 98 Est. number collected per foot 3 5 Est. number collected per minute 52 93 A-14 W o w Macroinvertebrate Taxonomic Data High Ore Ck. 970904 Rl Taxon % BI FFG Nematoda TOTAL: MISC. TAXA Ochrotrichia Rhyacophila Angelita Gr. TOTAL: TRICHOPTERA Ceratopogonidae Chelifera TOTAL: DIPTERA Diamesa TOTAL: CHIRONOMIDAE GRAND TOTAL 1 10.00 1 10.00 1 10.00 4 40.00 5 50.00 1 10.00 1 10.00 2 20.00 2 20.00 2 20.00 10 100.00 OM 4 PH 0 PR 6 PR 5 PR CG a-i; Aquatic Macroinvertebrate Data: High Ore Creek, September 4, 1997. Sample: I % of sample used: 100 Subsample size 1 0 Taxa richness 6 EPT richness 2 Biotic index 3.00 % Dominant taxon 40 % EPT 50 % Collectors (g+f) 20 % Scrapers + Shredders 0 % Hydropsychinae of Trich 0 Metals tolerance index 3.9 Shannon Diversity (log2) 2.32 EPT/Chironomidae 2.5 CTQa undefined %Baetidae of Ephemeroptera 0 % Coleoptera 0 % Diptera 20 % Chironomidae 20 % Ephemeroptera 0 % Plecoptera 0 % Trichoptera 50 % multivoltine 32.5 % univoltine 47.5 % semivoltine 20 Functional Feeding Grp. %RA # taxa Filterers Collector-Gatherers Shredders Scrapers Predators Est. total number of organisms Est. number collected per foot Est. number collected per minute A-16 \^^^mf 0 0 20 1 0 0 0 0 60 10 <1 10 3 J Macroinvertebrate Taxonomic Data Little Boulder River 970912 Rl.l C Taxon # % BI FFG 01igochaeta:Enchytraeidae 21 6.38 10 CG TOTAL: MISC. TAXA Baetis tricaudatus Drunella doddsi Ephemerella inermis Epeorus longimanus Rhithrogena Paraleptophlebia heteronea Ameletus TOTAL: EPHEMEROPTERA Zapada cinctipes Zapada Oregonensis Gr. Doroneuria Megarcys Skwala TOTAL: PLECOPTERA Arctopsyche grandis Brachycentrus americanus Glossosoma Lepidostoma Apatania Dolophilodes Rhyacophila Betteni Gr. Rhyacophila Brunnea Gr. Rhyacophila Hyalinata Gr. TOTAL: TRICHOPTERA Heterlimnius Narpus Zaitzevia TOTAL: COLEOPTERA Ceratopogonidae Glutops Pericoma Hexatoma TOTAL: DIPTERA Brillia Cricotopus Nostococladius Eukiefferiella Brehmi Gp. Orthocladius Pagastia Rheocricotopus Rheotanytarsus Thienemanniella Tvetenia Bavarica Gr. TOTAL: CHIRONOMIDAE GRAND TOTAL A-17 21 6.38 21 6.38 7 2.13 10 3.04 4 1.22 1 0.30 49 14.89 2 0.61 1 0.30 74 22.49 41 12.46 1 0.30 3 0.91 5 1.52 1 0.30 51 15.50 14 4.26 10 3.04 77 23.40 2 0.61 3 0.91 7 2.13 1 0.30 8 2.43 1 0.30 123 37.39 34 10.33 1 0.30 3 0.91 38 11.55 2 0.61 1 0.30 1 0.30 2 0.61 6 1.82 2 0.61 3 0.91 2 0.61 2 0.61 1 0.30 1 0.30 1 0.30 1 0.30 3 0.91 16 4.86 329 100.00 4 CG 1 SC 4 sc 1 SC 0 sc 1 CG 0 CG 3 SH 2 SH 0 PR 1 PR 3 PR 2 CF 1 SC 0 SC 1 SH 3 SC 0 CF 0 PR 2 PR 0 PR 3 CG 2 SH 4 CG 6 PR 1 PR 4 CG 2 PR 4 SH 6 SH 8 CG 6 CG 1 CG 4 CG 6 CF 6 CG 5 CG Macroinvertebrate Taxonomic Data Little Boulder River 970912 R1.2 ^J Taxon % Bl FFG Oligochaeta: Enchytraeidae Acari TOTAL: MISC. TAXA Baetis tricaudatus Caudatella heterocaudata Drunella doddsi Drunella grandis Epeorus longimanus Epeorus grandis Rhithrogena Ameletus TOTAL: EPHEMEROPTERA Visoka cataractae Zapada cinctipes Zapada Oregonensis Gr. Hesperoperla pacifica Megarcys Skwala TOTAL: PLECOPTERA Arctopsyche grandis Brachycentrus americanus Micrasema Glossosoma Lepidostoma Apatania Dolophilodes Rhyacophila Brunnea Gr. Rhyacophila Hyalinata Gr. TOTAL: TRICHOPTERA Cleptelmis Heterlimnius Lara avara Zaitzevia TOTAL: COLEOPTERA Glutops Pericoma Simulium Antocha Hexatoma TOTAL: DIPTERA Brillia Corynoneura Cricotopus Nostococladius EukiefTeriella Brehmi Gp. Eukiefferiella Claripennis Gr. Symposiocladius TOTAL: CHIRONOMIDAE GRAND TOTAL 10 3.12 I'- 0.31 ll 3.43 3 0.93 12 3.74 12 3.74 1 0.31 2 0.62 1 0.31 38 11.84 1 0.31 70 21.81 2 0.62 38 11.84 2 0.62 3 0.93 3 0.93 1 0.31 49 15.26 19 5.92 19 5.92 11 3.43 70 21.81 3 0.93 2 0.62 14 4.36 3 0.93 1 0.31 142 44.24 11 3.43 13 4.05 1 0.31 3 0.93 28 8.72 1 0.31 1 0.31 1 0.31 1 0.31 2 0.62 6 1.87 2 0.62 1 0.31 6 1.87 4 1.25 1 0.31 1 0.31 15 4.67 321 100.00 A-18 10 CG 5 PA 4 CG 0 SC 1 SC 2 CG 1 SC 0 SC 0 SC 0 CG 0 SH 3 SH 2 SH 1 PR 1 PR 3 PR 2 CF 1 SC 1 SH 0 SC 1 SH 3 SC 0 CF 2 PR 0 PR 4 CG 3 CG 1 SH 4 CG 1 PR 4 CG 5 CF 3 CG 2 PR 4 SH 7 CG 6 SH 8 CG 8 CG 5 SH J J Aquatic Macroinvertebrate Data: Little Boulder River, September 12, 1997. Sample: 1.1 1.2 % of sample used: 40 50 Subsample size 329 321 Taxa richness EPT richness Biotic index % Dominant taxon % EPT % Collectors (g+f) % Scrapers + Shredders % Hydropsychinae of Trich Metals tolerance index Shannon Diversity (log2) EPT/Chironomidae CTQa %Baetidae of Ephemeroptera 38 21 2.09 23 75 31 62 0 2.37 3.85 15.5 56.27 9 39 23 1.72 22 81 26 69 0 2.37 4.12 17.4 52.74 4 % Coleoptera % Diptera % Chironomidae % Ephemeroptera % Plecoptera % Trichoptera 12 2 5 22 15 37 9 2 5 22 15 44 % multivoltine % univoltine % semivoltine 5 70 24 5 72 24 Functional Feeding Grp. %RA # taxa %RA # taxa Filterers Collector-Gatherers Shredders Scrapers Predators 7 24 15 47 7 3 13 6 7 9 11 16 21 49 4 3 12 9 8 7 Est. total number of organisms Est. number collected per foot Est. number collected per minute 823 34 329 642 32 642 A-19 J