eo Issued May 27, 1913. Us, DEPARTMENT OF ‘AGRICULTURE, BUREAU OF ENTOMOLOGY CIRCULAR /No. 173. L. O. HOWARD, Entomologist and Chief of Bureau. ARSENATE OF LEAD AS AN INSECTICIDE AGAINST THE TOBACCO HORNWORMS. BY A. C. MORGAN anp D. C. PARMAN, Entomological Assistants. é WASHINGTON : GOVERNMENT PRINTING OFFICE ; 1918 86647°—13 . BUREAU OF ENTOMOLOGY. LL. O. Howarp, Entomologist and Chief of Bureau. C. L. Martatr, Entomologist and Acting Chief in Absence of Chief. R. S. Cxiirton, Executive Assistant. W. F. Taster, Chief Clerk. F. H. Currrenpen, in charge of truckcrop and stored product insect investigations. A. D. Hopkins, in charge of forest insect investigations. W. D. Hunrer, in charge of southern field crop insect investigations. F. M. WesstTeER, in charge of cereal and forage insect investigations. A. L. QUAINTANCE, in charge of deciduous fruit insect investigations. BH. F. Pures, in charge of bee culture. D. M. Rocers, in charge of preventing spread of moths, field work. Roizia P. Currig, in charge of editorial work. MABEL CoLcorD, in charge of library. SouTHERN F1rLp Crop INSECT INVESTIGATIONS, W. D. HUNTER, in charge. W. D. Pierce, J. D. MircHett, G. D. SmitrH, E. A. McGrecor, Harry PINKUS. B. R. Coap, W. A. THomas, R. W. Moretanp, A. W. JOBBINS-PoMeRoy, C. E. Hester, engaged in cotton-boll weevil investigations. A. C. Moraan, G. A. Runner, S. E. Crums, D. C. PARMAN, engaged in tobacco insect investigations. F. C. BisHopp, A. H. JeEnninGs, H. P. Woop, W. V. Kine, engaged in tick investi- tions. T. BE. Hottoway, E. R. BaRBer, engaged in sugar-cane insect investigations. J. L. WEBB, engaged in rice insect investigations. R. A. Cootey, D. L. VAN Dine, A. F. Conrani, C. C. KRuMBHAAR, collaborators. (11) CIRCULAR No. 173. Issued May 27, 1913. United States Department of Agriculture, BUREAU OF ENTOMOLOGY. L. O. HOWARD, Entomologist and Chief of Bureau. ARSENATE OF LEAD AS AN INSECTICIDE AGAINST THE TOBACCO HORNWORMS. By A. C. Morcan and D. C. PARMAN, Entomological Assistants, INTRODUCTORY. During the past five years the Bureau of Entomology has been conducting an investigation of tobacco insects in Tennessee and Kentucky and in some of the adjoining States. In Tennessee the bureau has been very materially assisted by Prof. H. A. Morgan, director of the Tennessee Agricultural Experiment Station. In the dark-tobacco districts of Kentucky and Tennessee tobacco hornworms are the ever-present and most serious problem of the tobacco grower. ‘Ten to twelve years ago, when labor was plentiful, cheap, and efficient, “ hand-worming” was found to be economical and effective in combating this pest. However, during the last six or eight years hand-worming has become too costly, because of the great scarcity of labor, and too inefficient, and the growers have been forced to employ an insecticide. At the time insecticides were first used Paris green was found to be the safest and most efficient. Nev- ertheless, there has always been complaint of frequent serious burn- ing of tobacco as a result of its use. To find a safe and effective insecticide has been one of the main lines of investigation by the writers during the past five years. Di-plumbic arsenate of lead has been found to meet the requirements. In the further discussion of this subject the use and action of Paris green will be rather thor- oughly discussed in connection with the use and action of arsenate of lead, for the reason that since the insecticidal results of the use of Paris green are so well known it will be easier to explain the value of arsenate of lead if it be compared with this well-known poison. 1 2 ARSENATE OF LEAD AGAINST TOBACCO HORNWORMS. NECESSITY AND ADVANTAGES OF THE USE OF AN INSECTICIDE. The way in which the scarcity of labor tended to bring about the use of an insecticide upon tobacco has already been explained. In addition to this necessity of using insecticides, the much greater efficiency of a good application of an insecticide is another strong argument in its favor. Hand-worming, even of the best, has many objections; for instance, eggs are not picked off, many small worms are overlooked on account of their small size, and, lastly, during the hot hours of the day large worms craw] down into the “ruffles” near the bases of the leaves and a considerable number are thus over- looked. On the other hand, a thorough application of an insecti- cide will kill practically every hornworm—except those very nearly full grown—within two or three days, and will also continue to kill the young worms that hatch several days after the application. In short, hand-picking has only an immediate effect in lessening the worms, whereas the application of an insecticide usually continues to kill over a period-of several days. Cheapness is another point very greatly in favor of an insecticide as compared with hand-picking. The cost of keeping an acre of tobacco hand-wormed in a year when worms are plentiful is variously estimated at from $6 to $10. A like number of worms can be killed with an insecticide at a cost of not more than $2 or $3 an acre—sometimes less. THE USE OF PARIS GREEN. In some districts of Kentucky Paris green has been in use for over a decade. In the dark-tobacco districts of Kentucky and Tennessee it certainly was used to some extent 10 years ago and at the present time is in very general use. On account of the frequent injury to tobacco by the use of this insecticide many farmers would not use it if labor could be secured to do the hand-picking. On the whole, the cost of the Paris green plus the cost of application, plus the loss due to damaged tobacco, is much less than the cost of hand-worming. In this district the use of insecticides has come to stay. It is a neces- sity. Paris green is applied with a dust gun and without a carrier. From 1 to 2 pounds per acre is the usual application; 1 pound when worms are small (i. e., less than half grown) and 2 pounds when there are many worms over half grown. Success with the applica- tion depends upon the judgment of the farmer in choosing the time of application and upon the thoroughness with which the application is made. Much of the tobacco that is injured by Paris green is in- jured because of unevenness of application, or, what is too frequently the case, because the grower has delayed the application until half- ARSENATE OF LEAD AGAINST TOBACCO HORNWORMS, 3 grown or two-thirds grown worms have become dangerously nu- merous, and has then put on a large and uneven application with the hope that he would kill all the large worms. This is an example of poor judgment. Two applications should have been made. The first should have been smaller and at an earlier date in order to kill the worms while small, and also to lessen the danger of burning the tobacco. The second application should follow as soon as worms begin to increase in numbers after the first application. @e OBJECTIONS TO THE USE OF PARIS GREEN, DANGER TO THE OPERATOR, There is occasionally some injury or irritation to the operator in applying Paris green. Wherever Paris green strikes the tender parts of the body irritation soon occurs unless a thorough bath be taken promptly. Bleeding at the nose sometimes occurs as a result of the irritation to the mucous membranes. For these reasons many people dislike to apply Paris green. However, if care is taken to work in a direction quartering the breeze and upon the windward side of the row while making an application, a thick sack around the body and a sponge over the nose will be found to be excellent preventives of irritation, particularly if followed by a bath. INJURY BY PARIS GREEN TO TOBACCO. The very general complaint of loss due to Paris-green-burned to- bacco has been found to be justified. Under favorable weather con- ditions 2 pounds of Paris green per acre, sometimes more, may be applied without noticeable injury. On the other hand, unfavorable weather conditions will frequently cause injury to follow an appli- cation of only 1 pound per acre. Very hot suns and low humidity for an extended period will produce a condition of the tobacco plant very susceptible to Paris-green burn. Light rains or very heavy dews immediately following an application will wash the Paris green down into the axils of the leaves or into the furrows along the mid- ribs, and serious injury is likely to result. In the Clarksville district of the dark-tobacco belt of Kentucky and Tennessee Paris-green burn was quite severe during 1911 and 1912, particularly so in 1912. Previous to these years one of the most careful growers in this district informed the writers that his loss on a 6-acre field of tobacco, due to Paris-green burn, amounted to 8 per cent gross, which was a loss of at least 16 per cent of the net profit, and that many other growers suffered a similar loss in 1912. In 1911 the writers observed many fields in which the loss equaled or exceeded that quoted above. In 1912, however, the injury by Paris green was more widespread than for several years, notwithstanding 4 ARSENATE OF LEAD AGAINST TOBACCO HORNWORMS. the fact that many growers who suffered loss the year before were extremely careful in making applications. On January 4, 1913, the senior writer interviewed several tobacco buyers employed by the Italian Government. He was informed that several crops of tobacco the grade of which was especially suitable for the Italian Government were not bid upon because of the large percentage of tobacco injured by Paris green. The buyer stated that for one crop, had it been in average condition in regard to Paris- green injury, he would have bid 83 cents per pound. In its damaged condition, however, he did not believe the crop was worth over 5 cents per pound—a gross loss of 34 cents per pound, or of $25 to $30 per acre. Another crop would have received an offer of 8 cents per pound from the Italian buyers, but on account of Paris-green burn no offer was made. This buyer thought the crop worth not more than 54 cents—a gross loss of 24 cents per pound, or about $20 per acre. A third crop had its value reduced by one-half, a fourth crop was apparently worth about 5 cents per pound, and would ordinarily have brought 9 cents. This is the report of buyers of the heavier types of tobacco grown in this district. The lighter-bodied tobaccos undoubt- edly suffered as severely and probably more severely than the heavier tobaccos, because the lighter tobaccos are ordinarily more susceptible to Paris-green injury. Paris green injures tobacco in two ways: First, by causing dead, burned areas upon the leaves, where the powder has been collected by the dews or washed down by the rains; second, by weakening the leaf at the stalk. Light rains wash the insecticide into the axils of the leaves, and the result is that many leaves drop off before cutting time or become so weakened that they drop off when the plant is cut. Such leaves are not a total loss, for they are collected and cured, but they are a partial loss. They are light in weight and lack gloss and elasticity. THE USE OF ARSENATE OF LEAD. Arsenate of lead causes none of the injury mentioned above. Ex- periments performed under the direction of the senior writer showed that powdered arsenate of lead may be put on a fresh sucker wound in large quantities without causing any noticeable injury, and that when applied to a torn or bruised leaf it produces no injury. Paris green can not be applied to tobacco in the “graining” stage (i. e.. when nearly ripe) in sufficient quantities to do good insecticidal work without too grave danger of burning the plant. Arsenate of lead, on the other hand, can be safely applied to tobacco in the “ graining ” stage In quantities sufficient to produce satisfactory insecticidal re- sults. Furthermore, arsenate of lead will cause no irritation to the ARSENATE OF LEAD AGAINST TOBACCO HORNWORMS. 5 operator as will Paris green; in fact, thus far it has produced no noticeable injurious effects upon the operators. Since arsenate of lead can be applied to tobacco without injuring the plant, and since it is very much less objectionable from the oper- ator’s standpoint, its insecticidal properties should next be discussed. As the dosage and action of Paris green are very widely known, the value of arsenate of lead as an insecticide can the more easily be explained by comparing it with Paris green. The following tables will serve to show the relative values of the two insecticides under different conditions. TABLE I.—Comparison of the insecticidal effects against hornworms of arsenate of lead and Paris green in fair weather. S Examinations to show number of worms killed. z ; On sec- On On On : = o. On day | On first | “ ond third | fourth | fifth | AZ a i pape mE Poison used. ea Bea day day day day oe E PP i ie IABESe * | after. | after. | after. | after. | °g ane 3 ica) Z HA/AA IAlS/AIS/AlS/A/a/AIlG4 1 | Aug. 25, 1910 5 | Lead arsenate./134 | 0 | 46 | 56 | 24 | 22 |....|.... 1D 22 AL Eesti Ase 50 Pe ae Ob 2 iae5 BP sal eee Obs acss2- 0} Ons | GL sori oliee- sje. UTA Vee eae ees 50 3 | Aug. 24,1910 13 | Paris green....|106 | 0} 35 | 49 | 14) 17) 5 (al SS) eer) (Go) eC 50 4} Aug. 21,1911 42 | Lead arsenate .|135 5 | 92 | 45 | 70} 20} 35 | 23 | 31 | 26 |....}..-. 100 5 | Aug. 23,1911 13 | Paris green....|107 | 7 | 86) 17 | 61] 16 | 50] 15} 52] 8 |-.-.]-..- 100 1 Taken on 200 plants by ordinary hand-worming. 2 Many small worms alive. Arsenate-of-lead experiments Nos. 1 and 2 were applied under very favorable conditions, i. e., there was dew upon the plants and no breeze. Paris-green application No. 3 was applied under equally favorable conditions. These three experiments killed worms very satisfactorily. The records in Table I were made by counting the worms on 50 plants of tobacco on each succeeding day after the ap- plication. No. 1, 5-pound dosage of arsenate of lead, gave the best results, for on the fourth day after the application only 2 live worms were found in hand-worming 200 hills. The 34-pound dosage of arsenate of lead was not quite so good, although only 14 live worms were found on 200 plants the fourth day after the application. The Paris-green application No. 3 killed more quickly than either of the applications of arsenate of lead, but on the fifth day after the appli- cation numerous small worms were noticed in worming 200 plants. It was thus apparent that the Paris green was losing its effect, owing to heavy dews which tended to puddle it, and to heavy drying winds during the day, which blew some of it from the plants. The application in experiment No. 4 was not made under the most favorable conditions. There was a slight breeze during the appli- 6 ARSENATE OF LEAD AGAINST TOBACCO HORNWORMS. cation; in addition, there were a large number of eggs on the plants, . and many of the young worms hatching from these eggs were not killed until they wandered from the place of hatching. The same is true of experiment No. 5, the 13-pound dosage of Paris green. It will be seen, however, that experiment No. 4, the arsenate-of-lead application, was more effective than the Paris-green application, for on the day of application there were at least 140 worms in experi- ment No. 4, the arsenate-of-lead application, and only 114 worms in experiment No. 5, the Paris-green application, while on the fourth day after the applications there were only 31 live worms in experi- ment No. 4, but 52 in experiment No. 5. The poisons were applied in the following series of tests in the morning from 6.30 to 9 o’clock. The arsenate of lead was mixed with an equal weight of dry wood ashes. All applications were made with fan dust guns. The mixture of ashes and lead arsenate made a very good dust and compared favorably in evenness with the application of Paris green. Rain began to fall at 11.30 a. m. and continued in- termittently until2 p.m. Part of the rain was dashing. About one- third of an inch fell. The first examination of the plats was made after 3 p.m. of the same day. The tobacco on these plats was nearly full grown and lapped in the rows considerably. TABLE II.—A comparison of the insecticidal effects against hormworms of arsenate of lead and Paris green in rainy weather. Examinations to show number of worms On On On On bi second } third | fourth Size of ° P Date of . On da = application. |Posage-| Poison used. | py niied. is day day day | worms left. FB 2 after. after. after. & 3. SISIEIZIEISIEISIEIS al =| o | = oo; o| a o <3) “AIAIS IAS IAIlSAIAI<4/A Pounds per acre. 1] Aug. 28,1911 f Lead arsenate.| 59 | 49 | 15} 41/10] 8 ]....].... 6 | 2 Small. Albans cha ped CR Radiisn a2. Goer es cEO2, |UD | AR Ar awe Bed. Ie scot rear 33 8| Small and medium. af oes GOs | eation. a a day ay ay = mB plied after. after. | after after E eee i] 4 Glo lOlololsololgijolad io q > a > a > a 5 a > a ial i) a o|5 o|] a | eee o|} o ea) Ay “j}A/sa/AS