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CONTENTS.
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THE GRASSHOPPER. 5
THE ABDOMEN.
Count the abdominal rings.
Observe two grooves running along the under surface
of the abdomen. The under part of the abdomen,
included between these grooves, is the sternum, the
side of the abdomen is called the pleurum, and the
upper part is the tergum; the corresponding parts of
each separate ring are the sternite, pleurite, and
tergite.
Just above the groove which separates the sternum
from the pleurum is a row of small holes, the breath-
ing pores, or spiracles; count them.
In a live specimen, watch the movements of breathing.
All insects breathe by means of a complicated system
of air tubes, the trachez, which branch from the
spiracles throughout the body. Can the grasshopper
be drowned by holding its head under water? Connect-
ed with the air tubes, in grasshoppers and other strong
flying insects, as bees and flies, are large air sacs, which
fill with air, and are said to aid, like little balloons,
in keeping the insect in the air. By carefully cutting
away the roof of the abdomen, these air sacs may be
seen, marked by their white walls; the white air
tubes, or tracheze, may also be readily seen.
Under the bases of the wings, on the first abdominal
ring, is a pair of thin, shiny, oval membranes, the
tympana, or ear drums. The inner surface of each
tympanum is connected with a nerve; but several
investigators have denied the auditory nature of this
apparatus. *
The abdomen of the female ends in four points; in
6 PRACTICAL ZOOLOGY.
laying the egg these points are first pressed together,
then thrust into the ground, and then separated; this
process is repeated till a hole is made, sometimes as
deep as the abdomen is long, at the bottom of which the
eggs are deposited, passing out between the four points
of these egg guides, which together are called the
ovipositor; compare the inner and outer surfaces of
these egg guides. The males are smaller than the
females. Draw the abdomens, as seen from the side,
of both the male and the female. Take now an entire
specimen and draw a side view of it.
INTERNAL STRUCTURE OF THE GRASSHOPPER.
This work would better be done after the student has
dissected the crayfish. Dissect under water with the dis-
secting pan as described under the “ crayfish.” ;
1. Get a large female grasshopper, freshly killed. Cut
off the wings, and place the specimen, back upper-
most, in the dissecting pan; pin the hindermost ring
of the abdomen firmly to the bottom of the dissecting
pan; turn each hind leg outward and pin down.
With sharp, fine-pointed scissors, cut through each
side of the roof of the next to the last abdominal ring;
lift, with the forceps, the cover of this ring; continue
the cut forward, on each side of the abdomen, pulling
the tergum upward and forward as it is loosened.
Thus carefully unroof the whole abdomen.
2. The heart is a delicate tube, running along just
under the tergum, and probably was torn away with
the tergum.
8. On each side there should now be seen a row of air
sacs, with their white air tubes.
—_
—_—
THE GRASSHOPPER. 7
4. In the anterior part of the abdomen a mass of yellow
eggs is usually to be found; this mass may be easily
separated into two parts, right and left, from each of
which a tube, oviduct, leads to an opening between
the parts of the ovipositor.
5. Under the eggs is the dark intestine, running length-
wise.
6. Remove the roof of the thorax; more air sacs should
be found here. In the upper part of the thorax are
the white muscles which move the wings. Removy-
ing these muscles exposes more of the digestive tube;
as the food is swallowed, it passes upward in a brown
tube, which soon turns backward into the thorax; in
the prothorax, the enlargement is the crop, in which
is produced the dark liquid which the grasshopper
ejects from the mouth when held captive. The crop
may be removed, washed, split open and examined
under the microscope with a half-inch objective to show
the rows of hooked teeth with which it is provided.
A little further back the digestive tube is surrounded
by a set of double cone-shape pouches, which extend
parallel with the main channel of the digestive tube.
These are the gastric ceca. Behind them is the
stomach, followed by the intestine. The products of
digestion pass through the coatings of the digestive
canal, and mingle with the currents of blood which
pass along the ventral and lateral parts of the body.
7. The coloriess blood enters the heart through holes
along its sides; blood is sent from the heart into the
veins of the wings. These veins are hollow tubes,
and though they convey blood, are very different
from the veins in our bodies. Air tubes run along
8 PRACTICAL ZOOLOGY.
the centre of the larger veins, and give air to the
blood as it flows.
8. The nervous system of the grasshopper consists mainly
of a white cord extending along the floor of the whole
body cavity. In most of the abdominal rings the
nerve cord has enlargements called ganglia, from
which nerves branch to the surrounding parts.
THE DEVELOPMENT OF THE GRASSHOPPER.
The egg hatches out a little grasshopper, at first with-
out wings. As it grows, it sheds its skin (moults) several
times. In moulting, the skin splits along the back of the
head and thorax, and the insect works its way out. At
first the newly hatched insect is very soft; the writer has
seen a grasshopper bend its tibia double in the effort of
pulling out of the old skin; but the tibia soon straight-
ened and hardened, showing no signs of injury.
For descriptions of the grasshopper, see Packard’s “ Zodl-
ogy,” Packard’s “Guide to the Study of Insects,” Brooks’
“Handbook of Invertebrate Zodlogy,” “The Rocky Moun-
tain Locust,” in First Annual Report of U.S. Entomologi-
cal Commission, 1877 (issued 1878), Comstock’s “ Guide
to Practical Work in Elementary Entomology.”
GRASSHOPPER CARD.
Take a card six inches by four. Make a faint mark
lengthwise in the middle to aid in placing the parts sym-
metrically. Separate the parts of the grasshopper, and
paste them on the card in their proper order. Before be-
ginning, plan the whole arrangement. First, cut off the
head; leaving a central place for the head, remove the
mouth parts, pasting each to the card as it is removed.
THE CRICKET. 9
In separating the parts use the forceps, being careful to
get hold of the very base of each piece; then, holding
each part with the forceps, dip the side that is to go next
to the paper into the mucilage, and carefully place just
where it is to stay. This method avoids smearing the card.
Avoid getting too much mucilage. The mouth parts should
- surround the head; the wings should be opposite the parts
to which they were attached, as also the legs. The legs
should be separated to show all the segments; the thorax
should be separated into its parts, but the abdomen would
better be kept entire. As the parts become very brittle
when dry, it is well, if the card is to be kept, to make a
little bridge of a strip of paper, on which to string the
rings of the thorax and abdomen? The soft parts should,
of course, be removed.
THE CRICKET.
1. In what are the cricket and grasshopper alike?
2. In what respects do they differ ?
3. The female cricket has a long, slender ovipositor.
Compare its parts with the parts of the grasshopper’s
ovipositor, picking them apart with a dissecting
needle. Use a lens.
4, A pair of tapering, jointed projections from the abdo-
men are the stylets.
5. Compare the wings of the male and female. Look on
the under surface of the outer wings of the male for
a vein, running crosswise, near the anterior end, which
has on it a row of teeth. By rubbing this file on the
10 PRACTICAL ZOOLOGY.
veins of the other wing, the cricket makes its chirping
noise. Watch crickets to see how the wings are man-
aged during this process.
6. With a lens look for the so-called hearing organ on
the tibia of the fore leg.
7. Make a drawing showing all that can be seen from
above (dorsal view), and naming all the parts shown.
Grasshoppers and crickets both belong to the order of
insects called Orthoptera, or straight-winged insects.
e
THE BUMBLE BEE.
1. Find three ocelli on the top of the head. How are
they arranged ? .
Describe the antenne.
3. The mouth parts : —
bo
a. are the arms, orrays. Note that the rays are bilater-
ally symmetrical.
2. The mouth is at the center of a thin membrane in the
middle of the oral surface; the opposite surface is
called aboral.
3. Cut into one of the rays. Observe that the body
cavity is bounded by a leathery wall in which are im-
bedded hard plates. Compare a piece of a ray of an
_ aleoholic specimen with the dried one.
4. Test the flexibility of the integument of the alcoholic
specimen. By picking with forceps, prove that there
is soft matter, both on the outside and on the inside
of the hard plates. To show the real nature of the
plates and their relation to the integument, proceed
as follows: —
a. Handle a starfish which has been decalcified, 7.e.
has had its calcareous matter removed by very
weak (two per cent) nitric acid, chromic or other
152
PRACTICAL ZOOLOGY.
acid. Observe that the body wall is still present
but lacks the hard parts.
b. Examine a microscopic section of a decalcified ray
of a young starfish; in such section it should be
more clearly seen that the calcareous plates are
wholly within the integument.
To show still further the relation between the
plates and the integument, prepare a thin section
of a calcareous plate, as follows: select some pieces
of a starfish (left from previous dissection). Boil
a few of the larger plates in caustic potash in order
to remove all the organic matter; wash, and when
thoroughly dry, smooth down one side on a fine
file; polish on a perfectly clean oil-stone ; cement
this surface of the plate to a glass slide by means
of a drop of Canada balsam which has been boiled
on the slide, until on becoming cold it is with
difficulty indented by the thumb-nail. Proceed
then to plane off, by means of a file, and when
quite thin, scrape carefully with a sharp knife,
finally smoothing it on an oil-stone. The speci-
men should be examined from time to time under
the microscope, in order to ascertain when the
proper degree of thinness has been reached. Dis-
solve the balsam by means of turpentine, or better,
if properly managed, melt the balsam over a lamp
and carefully push the section into a watch-crys-
tal containing turpentine ; when thoroughly freed
from balsam, carefully brush it with a camel’s-
hair brush and mount in Canada balsam in the
ordinary manner.
5. Observe the arrangement of the plates and spines in
y
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THE STARFISH. 153
different regions of the body wall. Along the middle
of the oral surface of each ray may be seen the
shrivelled remains of the tube feet, or ambulacra.
The region in which they lie is the ambulacral area.
The plates along this tract are the ambulacral plates.
One row of plates on each side of these ambulacral
plates are known as the inter-ambulacral plates. Ex-
amine these closely for comparison with the sea-urchin.
. The wart-like elevation on the aboral surface is the
madreporic body. Note that it is situated opposite
one of the inter-radial angles. Examine it with a lens.
. Make drawings of the oral and aboral surfaces of the
starfish.
ALCOHOLIC SPECIMEN.
. Briefly review the points noticed in examining the
dried specimen. Bend the rays; their flexibility is
now much less than in life.
. Compare the spines of different areas as to their shape,
size, and degree of mobility.
. Between the spines are soft, tapering projections, the
aboral tentacles.
. Observe a circle of projections surrounding the spines ;
delicately pinch them with the forceps to determine
their consistence; remove some of these bodies to
strong alcohol; mount temporarily in turpentine on a
slide, cover, and examine with a low power. ‘There
should be distinguished a short stalk bearing a pair of
pinchers; these bodies are the pedicellariz. In the
live starfish these pinchers may be seen continually
snapping; they are supposed to serve in removing
foreign matter from the body.
154 PRACTICAL ZOOLOGY.
5. The soft cylindrical projections along the median tract
of the oral surface of each ray, are the ambulacra or
tube feet. Remove one of them and examine it with
care. Note the arrangement of the series.
6. Press apart the tube feet and find running along the
median line of the ambulacral groove, a yellowish or
whitish ridge, the nerve of the ray. Trace it to the
soft membrane bordering the mouth, the peristome,
and find the nerve ring around the mouth.
7. Trace the nerves also to their outer ends and find a
reddish or yellowish elevation, the eye-spot, borne at
the base of a median terminal tentacle, resembling a
tube foot.
8. The eye-spot is borne on a distinct, but minute, plate.
Compare young and old specimens to see that what-
ever the size, this single ocular plate with its eye-spot
is always at the end of the ray. Count the ambula-
cral plates ina short andinalongray. Where do the
new plates develop?
DISSECTION OF THE STARFISH.
1. The ray opposite the madreporic body is the anterior
ray. Cut through its aboral wall near the outer end,
and from this point cut along the upper part of each
side of the ray, an inch or two toward the disk; raise
the flap thus freed, and, avoiding internal organs,
continue the cut on each side to the disk.
2. Attached to the aboral wall find a pair of elongated,
branched bodies, the hepatic czca. Note how each
cecum is held in place by the thin mesentery.
8. Along the middle line of the aboral wall, inside, is a
yellowish streak, the extensor muscle of the ray;
with forceps prove its general structure.
= ee
THE STARFISH. 155
Along each side of the ridge in the floor of the ray,
observe rows of thin-walled sacs, sometimes distended,
but more often collapsed in alcoholic specimens.
These are the ampullz, or ambulacral vesicles.
Watch the ampulle while pressing on the tube feet,
and vice versa. If a specimen injected with coloring
matter be at hand it should now be examined.
Near the base of the ray find, on each side, an elon-
gated body resembling a bunch of grapes, and of a
lighter color than the ceca; these are the reproduc-
tive bodies and are very much alike in appearance in
the two sexes, and only distinguishable by color or by
microscopic examination in the living specimens. Find
the point of attachment of one of them. The open-
ings in the inter-radial angle are not very evident.
Cut along the sides of the two rays lying on the right
and left of the anterior ray, connect the cuts at the
inter-radial angles, and turn back the cover of the
three rays and disk. Within the disk is the large,
thin-walled stomach. Examine this organ carefully.
Pass a blunt probe through the mouth and explore
its interior.
Observe the large lobes of the stomach extending a
short distance into the rays; lift one of these lobes
and trace the thin retractor muscles of the stomach
to the sides of the ridge in the ray.
In the live starfish the stomach is often found pro-
truded and surrounding a mussel or an oyster; after
digesting and absorbing its soft parts the stomach is
retracted.
Turning to the ceca of the anterior ray, trace them
toward the stomach; find the union of their tubes
156
10.
11.
12.
13.
14,
15.
PRACTICAL ZOOLOGY.
and the entrance of their common duct into the
stomach. Observe the place where this tube enters
the stomach, in reference to the corresponding lobe
of ‘the latter.
Carefully cut the mesentery along the aboral wall
and wholly free the czca of this ray from all attach-
ment above. Note that the mesentery is double.
Hold the starfish inverted and pour water through
the mouth into the stomach to show its shape.
In the other two rays which have been opened, cut
across the common ducts of the ceca close to the
stomach, and leave them attached to the aboral walls.
Find the extremely short intestine connecting the
stomach with the upper wall of the disk, near the
junction of the extensor muscles of the rays. Find,
also, some small branched appendages of the intestine.
The anal opening is minute.
Sever the intestine close to the aboral wall, cut across
the disk close to the madreporic body, and remove
entirely the roof of the disk and the three rays.
Make a drawing of the organs now exposed, show-
ing the ceca in one ray, the reproductive bodies in
another, and the ampulle in the third.
Thoroughly examine the stomach, and remove it after
cutting across the short esophagus.
The S-shaped stone canal may now be seen passing
downward from beneath the madreporic body.
Traced to its lower end, the stone canal may be found
to enter a membranous hollow ring, whose outer bor-
der rests against the inner surface of the hard parts
surrounding the mouth; this tube is the circum-oral
water-ring. Connected with its inner surface, find
18.
19.
16.
ele
THE STARFISH. 157
several pairs of pouches, which in the contracted
state are mere button-like projections. How many of
these are there, and are they all in pairs?
Observe also the pouches, like ampulle, connected
- with the upper part of the hard ring around the
mouth. Press on the water-ring at the level of the
peristome, and watch the effect of this action on these
last-named pouches or vesicles. Is there any connec-
tion between them and the water-ring ?
Enclosing the stone canal is a thin membrane, the
pericardium. Carefully tear it away. Alongside
the stone canal is a soft tube, the heart. In the live
starfish it may be seen to pulsate.
Cut across the middle of a ray in two places, about
an inch apart, and make a careful study of the part
included between the cuts. Remove the hepatic
ceca, observing again how they are suspended by the
mesenteries. Cut through the aboral wall in the
middle line and spread open the ring. Observe the
depressions in its inner surface; in the bottoms of
these depressions find small holes. What is the rela-
tion between these holes and the nearest structures
seen on the outside ?
Slowly peel away the thin membrane which lines the
interior of the ray, noting especially the connection
between this membrane and the depressions above
noticed. Also watch closely the aboral tentacles
while tearing away this lining membrane.
Turn now to the outside of the ray and gently scrape
the surface. A thin layer here may also be easily re-
moved. Thoroughly clean a small area, noting that
the aboral tentacles come away with this layer.
158
20.
21.
22.
24,
PRACTICAL ZOOLOGY.
There will now remain a tough white layer in which
are imbedded the calcareous plates which constitute
the skeleton. Bend this membrane to see the relations
of the calcareous plates to the membrane and to each
other.
By picking with the forceps prove that the membrane
is continuous over both the inner and outer surfaces
of the plates, as well as between them. This is an
important point, as the calcareous plates are devel-
oped in and by the membrane.
Part of the membrane, if not all, has the power of
contracting, by means of which motion is effected.
Note the perforations in the membrane in its thinner
portions between the plates where the aboral tenta-
cles passed out.
Reviewing what was noticed in the examination of
the inner and outer membranes, it will be evident
that the aboral tentacles are tubular extensions of the
body formed by the protrusion of the inner mem-
brane through the middle membrane, these tubes be-
ing covered by the outer membrane.
Turn now to the tube feet and their ampulle and
make out their relations to each other and to the ad-
jacent parts of the skeleton. The calcareous plates
which form the sides of the ambulacral groove are
the ambulacral plates.
. Pick away afew of the ampulle, and then the cor-
responding tube feet, comparing the arrangement of
the two. In this way clean the ambulacral plates
and examine them carefully.
Alternately press the ambulacral plates of the two
sides together and separate them to see the range of
20.
26.
THE STARFISH. 159
motion allowed by the joint. Observe the muscles
connecting the ambulacral plates of the opposite sides,
just inside of the nerve.
In the angle formed by the ambulacral plates, find the
cut-off end of the water-tube of the ray. Insert in
the end of this, the point of a drawn-out glass tube,
and inflate. When the ampulle are distended, press
on them with the finger and note the effect on the
tube feet; with a lens examine the distended ampulle.
In fresh specimens the ampulle may be injected with
a colored liquid or with gelatine to be kept as perma-
nent preparations. In such preparations and in a
microscopic section of a properly prepared ray, it may
be seen that the water-tube of the ray sends off side
branches to the tube feet, and also that the cavities of
the tube feet and ampulle are continuous. By the
contraction of the ampulle the tube feet are extended,
and by the muscles in their walls they are moved from
side to side and applied to-the surfaces on which the
starfish rests. The end is fixed by means of the sucker-
like disk at the tip of the foot to some foreign object;
then by the contraction of the tube feet, the starfish
pulls its body along.
The water finds its way through the madreporic
body into the stone canal, thence to the water-ring
around the mouth, and from this to the radial canals.
The water thus taken in probably serves for respira-
tion as well as for locomotion.
Make a drawing of a cross section of a ray, showing as
many as possible of the above noted points of structure.
A slide with a series of very small starfishes shows well
how the rays are formed as outgrowths of the disk.
160 — PRACTICAL ZOOLOGY.
For the anatomy and development of the starfish and
sea-urchin, see Brooks’ “ Handbook of Invertebrate Zodl-
ogy,’ Hyatt’s “Common Corals, Hydroids and Echino-
derms” (No. V. of “Guides for Science Teaching”),
“Seaside Studies in Natural History,” by E. C. and A.
Agassiz, Romanes’ “ Jellyfish, Starfish, and Sea-Urchins”
(Vol. XLIX. in the International Scientific Series).
THE SEA-URCHIN.
The requisites for this work are, cleaned skeletons, or
tests, alcoholic specimens, microscopic sections, etc., as in
the case of the starfish.
THE CLEANED TEST.
1. Observe the radial distribution: of the parts around an
axis, at one pole of which is a large opening.
At the opposite pole is a circular area composed of
several small plates, near the center of which is the
anal opening.
2. Note that the test is composed of distinct pieces or
plates. Put one of the plates into a little dilute acid
and note what occurs.
3. To make out the real nature of the skeleton, proceed
thus : —
a. Handle an entire decalcified specimen, 7.e., one
from which the calcareous matter has been re-
moved by chromic or other acid. Observe that
the body walls and.spines are still present.
THE SEA-URCHIN. 161
6. Examine a microscopic section of the decalcified
body wall to see that there was soft living matter,
both on the outside and on the inside of the cal-
careous plates.
e. Grind down and mount a thin section of a plate,
as in the case of the starfish, and see that not
only is the plate wholly enclosed in the body wall,
but that it forms a network whose meshes were
penetrated by the soft living substance of that
body-wall. It should now be clear that the plates
were formed by the deposition of calcareous mat-
ter within the living tissues of the body wall.
The joints, or sutures, between the plates are
formed by the absence of the deposit of calcare-
ous matter.
. Returning to the entire test, study the arrangement
of the plates, their variations in shape, size, etc.
Into how many similar areas may the surface of the
test be divided? ‘To make out these points, and the
shapes of the plates, pull apart a piece of a dried test
that was left over from previous dissection.
. At the aboral pole, observe a small distinctly marked-
off area, including numerous small plates. This is
the anal area, and the plates are the anal plates.
Unlike the other plates, these, in the living sea-urchin,
are movable. They surround the anus.
Surrounding the anal area are the five large genital
plates, each having a genital opening near its outer
angle.
. With a lens examine the largest of the genital plates;
its perforated portion serves as a madreporic body.
. Radiating from the apex of each genital plate, is the
162
10.
11.
PRACTICAL ZOOLOGY.
zigzag inter-radial suture. How many kinds of
plates are found within the area included by two
adjacent inter-radial sutures? The perforated plates
are the ambulacral plates, and the unperforated, the
inter-ambulacral plates. Compare these two sets of
plates with the corresponding parts of a starfish.
The ambulacral plates form the ambulacral areas.
Trace each of the ambulacral areas to its aboral end,
and find at its apex a small plate wedged in between
two adjacent genital plates. These smaller ones are
the ocular plates. Note the small opening from
which projects an unpaired tentacle, the end of the
radial water-tube.
Carefully compare the hard parts of the starfish and
sea-urchin. Wherein are they alike, and wherein do
they differ? What changes in growth would be
necessary to convert one of these forms into the
other? What part of a starfish is homologous with
the anal area of a sea-urchin ?
Make careful drawings of the oral surface, of the
aboral surface, and of the side of the test.
ALCOHOLIC SPECIMEN.
For the sake of review and comparison, it is well to
have the cleaned test before one in this study.
1.
Observe the soft membrane, the peristome, on the
oral surface and the teeth projecting from the
mouth.
At the aboral pole look for the anus and genital
plates.
Examine one of the largest spines; move it about to
ee a
THE SEA-URCHIN. 163
see its range of motion. Remove it and make out
how it is articulated to the test. The fleshy tube en-
sheathing the base is muscular tissue, by the contrac-
tion of which the spine is moved. Clean the spine
and make a drawing of it.
4, Note any variations in size and shape of the spines in
various regions.
5. Study carefully the arrangement of the spines, using
the cleaned test for comparison.
6. Between the spines in certain areas find soft tubular
projections, the tube feet or ambulacra. In life they
may be extended a considerable distance beyond the
spines, being used for locomotion as in the starfish;
carefully examine the tips of the tube feet to find
what is therein contained.
7. Find also among the spines and on the peristome,
slender flexible stalks, bearing three-pronged pinch-
ers. In life these pinchers keep opening and shutting.
8. Pick away the spines and other projections prepara-
tory to dissection.
DISSECTION OF THE SEA-URCHIN.
After removing the spines, cut, or better, saw with the
blade of a metal saw, through the equator of the test;
place under water and carefully raise the aboral portion
at one side.
1. Press on the tips of the teeth to show their connec-
tion with the complicated apparatus known as the
lantern; now open the test till the two halves are side
by side and complete the dissection under water.
2. Arising from the middle of the inner surface of the
lantern find the brown esophagus. ‘Trace this as it
164 PRACTICAL ZOOLOGY.
passes in festoons about the body walls, widening te
become the stomach. ‘Trace the intestine to the anus,
describing carefully its course.
3. Pick away the alimentary canal from the oral half of
the test. Note the five double rows of ampulle; be-
tween each of these double rows runs the radial
water-tube, and between the water-tube and the test,
is the radial nerve.
4, In the aboral half, note the reproductive bodies in the
loops of the intestine. Trace their ducts to the geni-
tal pores.
5. After cleaning away the intestine and reproductive
-bodies, trace the ampulle as they converge to the
ocular plate. Compare the inside and outside of the
test to see if the ampulle are really opposite the am-
bulacral pores noticed in the dry test.
6. Study the lantern, make out how it is supported, and
how its various parts are moved, and how they are
used.
Place in water the pieces of tests left after dissec-
tion and macerate till the spines are readily detached.
Then clean and keep them for the next class. They
will be useful for pulling to pieces to make out the
structure of the test. The sea-urchin and the star-
fish may be taken as convenient types of the branch
Echinodermata. ‘To this group also belong the Brit-
tle Stars, Holothurians, and Crinoids.
’ ’ o~
THE DEVELOPMENT OF THE SEA-URCHIN.
The reproductive bodies are very much alike in the
two sexes, distinguishable only by color or by microscopic
examination.
THE SEA-URCHIN. 165
In the dark-colored sea-urchin (Arbacia punctulata) the
ovaries are red, from the color of the contained eggs,
while the testes are white. Through the genital open-
ings already observed, there passes out into the water
from the female a multitude of red spherical eggs. From
the male there passes into the water a white liquid, which
on examination with a high power of the microscope is
seen to be composed of myriads of little bodies, the
spermatozoa, like slender tadpoles, and swimming by the
active vibration of their tails.
If these two elements meet in the water the egg may
be fertilized; otherwise, the egg does not develop, but
soon dies. This process of the fertilization and the
changes that the egg undergoes in consequence, have
been studied in the following manner :—
Live sea-urchins were opened, the ovaries and testes
removed, and torn open to let their contents escape. ‘The
ova and spermatozoa were mixed in a watch crystal of sea
water and watched under the microscope; the actively
swimming spermatozoa surround the ova; just how the
fertilization is accomplished is not fully known; it is
believed that a spermatozoon enters the ovum. After this
- the egg mass contracts, leaving a clear space around it
inside the outer coat, or cell wall; soon the egg mass
within divides into two equal parts, each of these halves
again divides into two, the four then become eight, six-
teen, thirty-two, and so on till the number can no longer
be counted and the egg lodks like a spherical mulberry.
This berry-like mass now becomes hollow, next one side is
pushed in like a rubber ball with one side punched in;
on the outside are little hair-like projections of the cells,
called cilia, which by their vibrations propel the body
166 PRACTICAL ZOOLOGY.
through the water. A set of needle-like rods develop
within, which soon make a skeleton shaped somewhat like
a common chair. This skeleton has a covering of soft
tissue, and the projections which correspond to the legs
of the chair are covered with strong cilia for locomotion.
The digestive tube has at first but one opening, that made
by the doubling-in of the outer wall, as above mentioned,
and the cavity of this depression forms the digestive
cavity. The mouth is formed later by a new opening
made through the outer wall into the first cavity and the
original opening becomes the anus. So far the young sea-
urchin is very unlike the adult; but after a time this larva
begins to transform into the real sea-urchin, and soon the
little sea-urchins, about the size of pins’ heads, are found
crawling up the sides of the glass vessels in which they
are kept.
The first of the changes here described should be care-
fully remembered, as this division, or segmentation, of
the egg is common to all but the very lowest animals,
though the manner of division may greatly vary.
THE FRESH-WATER HYDRA.
The fresh-water hydra has a cylindrical body, varying in
diameter from the size of a fine needle to that of a common
pin, and from one-fourth to one-half an inch in length. It
is found in fresh-water ponds and streams, usually attached
by one end to submerged stems, leaves, etc., frequently
on the under surface of a leaf. Surrounding the free end
of the hydra is a circle of thread-like appendages, the
tentacles, which often are longer than the body itself.
THE FRESH-WATER HYDRA. 167
Two species of hydras are found; one green, the other
brown or flesh-colored. Put the leaves and stems to
which the hydras are attached into shallow dishes, such as
fruit-dishes, and keep them in a light but shaded place;
watch their behavior when thus kept undisturbed. Cut
off a bit of leaf bearing a hydra, and transfer it to a deep
watch crystal half full of water. Without the aid of any
lens watch the hydra for several minutes. When it is
expanded, gently touch it with the tip of a pencil or other
blunt object.
Examine a hydra with a hand lens; are all parts colored
alike? Place the watch crystal on the stage of a micro-
scope and examine with a one-inch objective. The follow-
ing points of structure should now be made out: —
1. That the body is a hollow tube closed at one end and
open at the other. This opening, within the circle of
the tentacles, is the mouth.
2. That the tentacles are also hollow tubes, closed at
their outer ends, but at the inner communicating
freely with the body cavity.
3. That the body wall consists of two layers, which are
continuous with the corresponding layers of the ten-
tacles. How do these layers differ from each other? ©
The body is, then, a double-walled sac, and the ten-
tacles are simply extensions of this sac. Watch the
movements of the different parts of the body. Can
hydras move from place to place? If so, how is this
accomplished? Look in the body cavity for foreign
matter which has been taken in through the mouth
as food. Look also for minute particles obtained by
the digestion of such food matter. These particles
may often be seen in motion, caused by contractions
168 PRACTICAL ZOOLOGY.
of the body walls, or by the action of cilia lining the
body cavity. Look for knob-like extensions of the
side of the body. Buds are formed as outgrowths
of the body walls with a cavity continuous with the
body cavity. Place in a dish by itself with some
aquatic plants, a hydra bearing buds, and watch from
day to day the development of the bud into the form
of the parent. Observe the free circulation of food
material from the parent to the bud. Watch the
formation of tentacles. Look also for a thinning
away of the free end of the bud.
What is the greatest number of buds found on any
one specimen? Are buds borne on buds? By means
of a pipette transfer a hydra in a large drop of water
to aslide. Cut two strips of thick paper a quarter of
an inch long and one-sixteenth of an inch wide and
lay one on each side of the drop of water. Carefully
place the coverslip on the water, with its edges rest-
ing on the papers so as not to crush the specimen.
Examine now with a quarter or one-fifth inch
objective. Observe the cells of which the body walls —
are composed. Note the knotty appearance of the
tentacles. In these projections of the tentacles and ~
in the walls of the body are certain distinct oval
cells, the thread cells. Place a drop of acetic acid
on the slide at one edge of the coverslip, and touch |
the opposite edge of the coverslip with a piece of |
blotting paper, meanwhile watching the specimen |
closely. Examine carefully to see the thread-like
prolongations of the thread cells which have been
discharged as a result of the irritating acid. Small
animals coming in contact with the tentacles are
i-th
THE FRESH-WATER HYDRA. 169
paralyzed by means of these threads which are sud-
denly shot out; the tentacles then carry the victim
to the mouth, and it is swallowed.
Note the simplicity of the structure of hydra
—the absence of any distinct nervous system,
and all special organs of circulation and _ respira-
tion.
It is stated that hydras have been cut into
slices, lengthwise and crosswise, and each part not
only continued to live but grew into a_ perfect
hydra.
Besides multiplying by budding, hydra also pro-
duces ova and spermatozoa in projections of the body
walls. Both kinds of sexual elements are produced
in the same individual. Such an animal is called a
hermaphrodite.
There is a large group of animals, almost without
exception marine, constructed on essentially the same
plan as hydra, though often much more complicated.
Hence the hydra is the type of the group known as
the Hydroids. Many of them live in colonies, as
if the young hydras, instead of dropping off from
the parent and becoming distinct individuals, re-
mained attached with a free communication between
them all. At least two distinct forms of individuals
are commonly found: —
a. A hydra-like form, devoted to obtaining and pre-
paring nourishment for the colony, hence called
the nutritive zooid.
6. Modified forms, producing the generative ele
ments, the generative zooids.
oe
170
PRACTICAL ZOOLOGY.
c. Besides these two are often found forms modified
for protection, etc. |
If a stained and mounted specimen of a campanu-
larian or other hydroid be at hand, it will be found |
very useful in showing these points.
The different kinds of individuals, though often
greatly modified, still show the essential plan of the
hydra. Some hydroids have a tube of hard material
developed by the outer layer, and at the base of the
colony some kinds secrete a layer of this material
incrusting the object on which the colony is borne.
Some forms spread by runners like strawberries.
One form is common on the shells inhabited by her-
mit crabs. Others are attached to seaweed, while still
others are dredged up from great depths of the ocean.
Among certain forms of hydroids the generative
zooid becomes peculiarly modified in form, and ulti-
mately becoming detached, is known as a free gener-
ative zooid, jelly-fish, or medusa. These jelly-fishes,
or meduse are usually either bell-shaped or umbrella-
shaped, the part answering to the top being called the
bell or disk. Corresponding to a short handle is the
manubrium. This has at its free end an opening,
the mouth. The handle is hollow, and communi-
cates with tubes radiating through the disk, answer-
ing to the umbrella rays. These tubes are connected
by a circular tube, extending around the margin of
the disk. Along this margin are tentacles and organs
for receiving impressions of light or sound. Most
jelly-fishes swim by contracting the umbrella-like disk.
Along the radiating tubes, or in the manubrium,
are borne the generative elements; the eggs develop
THE SEA-ANEMONE. 171
into hydra-like forms, which, on becoming attached,
give rise by a process of budding, to a hydroid colony,
some members of which assume a medusa form, thus
completing the cycle. This mode of development
has been called, though inappropriately, an alterna-
tion of generations. All jelly-fishes do not, however,
develop in this way. Jelly-fishes are richly supplied
with lasso-cells, and the larger ones sting severely,
being dangerous to bathers.
Read the description of Cyanea and other jelly-
fishes in ‘“ Seaside Studies.”
THE SEA-ANEMONE.
In its general form the sea-anemone resembles a hydra,
having a cylindrical hollow body attached by one end to
some foreign object, and at the free end a mouth sur-
rounded by tentacles. In its internal structure, however,
the sea-anemone presents some new features. The mouth,
instead of opening directly into the body cavity, as in the
-hydra, opens into a stomach which hangs like a bag sus-
pended in this cavity; the stomach has no bottom, but at
its lower end communicates freely with the body cavity.
The body wall and stomach may be represented by a
glove-finger with its tip cut off and the open end turned
back part way into the larger part of the finger.
The cavity of the body is divided into a series of radial
compartments by fleshy vertical partitions, the mesente-
ries, which extend inward from the body wall, some reach-
ing the stomach and being attached to it, others not ex-
172 PRACTICAL ZOOLOGY.
tending so far inward as the stomach. Each tentacle
communicates with one of these radial compartments, and
is to be regarded as a mere extension of part of the body
cavity.
Alcoholic specimens should be sliced transversely and
longitudinally. In a transverse section of the lower part
of the body there will be seen the body wall with a series
of partitions extending inward and ending in a free edge.
The section across the upper part of the body shows an
outer circle, the body wall, an inner circle, the stomach
wall, and, connecting the two, the radially arranged parti-
tions, or mesenteries. Like the hydroids, the sea-anemone
is well provided with lasso cells.
Food is taken into the mouth, digested in the stomach,
then passed, mixed with sea water, into the body cavity,
through which it is made to circulate by the contractions
of the body walls. The indigestible portions of the food
are expelled from the stomach through the mouth.
The tentacles are often brilliant and variegated in color;
and when the sea-anemone is expanded, it well proves the
fitness of its name. For a very interesting description of
these beautiful animals read Mrs. Agassiz’s little book, “ A
First Lesson in Natural History” (No. IV. in “ Guides for
Science Teaching ’’).
CORAL POLYPS.
The coral polyps are similar to the sea-anemone in their
general structure. They usually grow in colonies with
their bases connected by a continuous layer of living
matter, from which the polyps grow by budding.
CORAL POLYPS. — STONY CORALS. 173
Through this common base the cavities of the polyps
communicate, more or less directly, so that food obtained
by one may nourish the whole colony. The coral polyps
also differ from the sea-anemone in forming a deposit of
hard matter. Representatives of the two kinds of coral
should now be examined.
STONY CORALS.
(Corals Proper.)
In a piece of stony coral, or compound skeleton of a
colony of coral polyps (Galazea is a good form to study),
make out the following points : —
1. The nature of the material itself; test by putting a
very small piece into weak acid, or by touching the
specimen with a drop of acid.
2. The cup, or theca, formed by an individual polyp,
often traceable as a long tube. Observe, —
a. The outer wall of the cup.
b. The partitions, or septa, extending inward from
the wall of the cup.
8. Between the cups, the porous limy secretion, which
was secreted by the common body substance, or cee-
nosarc, connecting the individual polyps.
Imagine the sea-anemone depositing limy matter in
the base of its body wall, forming a cup; fleshy radiai
ridges rising from the floor and wall of the cup be-
tween the mesenteries, and a similar deposit in these
ridges; thus it will be seen how the cup is formed by
174 PRACTICAL ZOOLOGY.
the individual polyp. By the continued growth of
the polyp, and the continuation of the limy deposit,
the cup becomes an elongated tube. By budding are
formed the branches of these tubes, increasing in size
and in the number of partitions as they grow.
4, Between the cups, a porous secretion of the same ma-
terial as that in the cups. This is deposited in the
common fleshy base, filling up, in some forms, the
spaces between the cups; and when one polyp dies, its
cup is covered over and buried out of sight by this
secretion of the common base.
5. Make a drawing of a mass of stony coral, showing
the general arrangement of the cups, their mode of
branching, and the common secretion between them.
6. Draw a cup as seen from its free end. Make also a .
drawing of a cross-section of the same cup toward
the smaller end.
In the stony corals the mesenteries are always in .
pairs, and the fleshy ridges, in which are secreted the
septa, arise between them. |
The tentacles are generally in multiples of six, and '
are not fringed. It is of this kind of coral that the ;
reefs are formed.
a
SEA-FEATHER, OR SEA-FAN.
In a sea-feather, e.g., Muricea, note : —
1. An outer bark-like layer; with the thumb-nail scrape
off a little of this layer and pulverize it between the
thumb and finger; mix this powder with water and
examine under a microscope. A better way to see
SEA-FEATHER, OR SEA-FAN. 175
the spicules is, to thoroughly clean them by boiling
some of the outer layer in caustic potash. In this
layer are holes from which the polyps protruded. In
this form, then, the secretion is wholly in the living
matter between the polyps, the bark-like layer being
composed of the dried flesh in which the spicules lie
imbedded.
Strip off a piece of the bark-like layer and note the
grooves on its inner surface. By examining the end
of this piece it may be seen that these grooves are
caused by a series of tubes running lengthwise near
the inner surface of this layer. Find the openings of
the tubes where they were broken; these tubes con-
nect the polyps of the colony.
. Thecentral axis of horn-like substance. Test its flexi-
bility and strength. Observe the grooves on its sur-
face, and the relation between them and the tubes
above noted. This horny axis is excreted by the
walls of these tubes, and is not penetrated by liv-
ing matter like the outer layer. In the precious red
coral the central axis is formed in the same way, but
is calcareous instead of horny, and the outer bark-like
layer has been removed.
. Note the mode of branching in a sea-fan, comparing
the margin with the central portion to see how the
meshes are formed. Remove some of the outer layer,
and compare with the sea-feather. In this group
(including sea-feathers, sea-fans, the precious red coral,
etc.) each polyp has eight fringed tentacles; also
eight mesenteries, which are never in pairs. An alco-
holic specimen, with the polyps expanded, should, if
possible, be examined.
176
PRACTICAL ZOOLOGY.
The hydroids, jelly-fishes, sea-anemones, and coral
polyps, with many other interesting forms, belong to
the branch Coelenterata. The celenterates are many-
celled, radially symmetrical animals, and never pos-
sess a digestive tube wholly cut off from the body
cavity.
SPONGES.
Each pupil should have a small specimen of a commer-
cial
sponge, showing large holes at the top, but not with
large holes running straight through.
The teacher will need several specimens of larger
sponges; one of the simple calcareous sponges, in alco-
hol;
a piece of a commercial sponge in alcohol, showing
the sponge-flesh still in place; a silicious sponge; and
slides showing sponge spicules.
The pupil should make out the following points from
his specimen : —
A;
Its elasticity; test first the specimen dry, and again
after wetting it. Compare the elasticity of different
kinds of sponges.
The fibrous structure; with forceps tear off a bit of
the sponge and examine with a lens. Then examine
under the microscope.
The sponge was attached by its basal surface to rock.
Find where it has been trimmed away with shears;
perhaps if this has not been thoroughly done, some
bits of rock may be found clinging to the base.
Examine now the different channels by which the
sponge is perforated.
SPONGES. 177
a. Large crater-like tubes, opening at the top of the
sponge. Looking into these, it may be seen that
they give off branches. If you can see right
through the sponge by looking into these open-
ings, you may know that too much of the base
has been cut away, and your specimen is not a
good one. With a razor or sharp knife, cut the
sponge in two down one of these large tubes, and
examine from the inside.
6. Trace the branches of the large tubes by gently
pushing into them a probe (a wire with a little
knob on one end). These lead, usually, to holes
seen on the outside.
e. Grooves on the surface of the sponge, some shal-
low, others already becoming enclosed by the
union of the tufts of fibres outside of them; in
this way is formed another set of tubes (d).
d. Tubes running parallel to the surface of the
sponge, whose cut-off ends may be seen near the
margins of the split sponge. Hold the half
sponge up to the light to see the radiating fibres
and the concentric series of holes indicating the
mode of growth of the sponge.
e. Minute branches of the above tubes penetrating
the sponge in all directions.
It must be borne in mind that the sponges we buy
are only the skeletons of sponges. In the living
sponge the skeleton is entirely imbedded in soft liv-
ing matter, and the skeleton cannot be seen on the
exterior; in fact, its fibres are not very evident in a
section of a fresh sponge. The outside of the sponges
whose skeletons we buy, when alive resembles, in color
178
“PRACTICAL ZOOLOGY.
and general appearance, the back of a kid glove, vary-
ing from dark reddish-brown to almost black. The
consistency of the living sponge is about the same as
that of beef liver. If one of these live sponges be
watched, a current of water is found to come out of
the larger holes at the top, and currents pass in
through the numerous smaller holes on the exterior.
If the sponge be handled, many of the smaller holes
close and entirely disappear.
In order to understand a little more clearly the
structure of the common sponge, and to see how the
currents of water are maintained, an examination of a
simple sponge will be useful. Our simplest sponges
have no elastic skeleton composed of horny fibres like
those of the commercial sponge, but have little needle-
shaped and three-pronged spicules of limy matter.
One form common on the northern Atlantic coast
is a simple or branched white tube, an inch or so in
height and sometimes as thick as a pigeon’s quill.
These are in clusters, attached by one end and open at
the other. Imbedded in the wall of each tube are the
spicules above mentioned, projecting both on the out-
side and on the inside. The inside of the tube is lined
with cells bearing cilia which, by their vibration, drive
the contained water out of the mouth of the tube; to
replace which, water enters through many holes which
pierce the wall of the tube. In sponges a little more
complicated, the cilia, instead of lining the main tube,
are limited to small pouches, or lateral branches of the
main tube, extending into the body wall and communi-
cating with the exterior through small pores. In others
the cilia are found only in certain enlarged portions
SPONGES. 179
of these radiating tubes. This represents the condi-
tion in the commercial sponges; certain cavities are
lined with cilia and are connected on the one hand
with the smaller tubes entering the whole surface of
the sponge, and on the other with the large tubes
opening at the top. These cilia cause the currents
above mentioned. Thus the sponge gets both food
and oxygen. cs
Sponges (including, besides those already men-
tioned, silicious sponges, whose spicules are flinty)
constitute the branch Porifera.
For a very interesting account of the gathering and
preparation of sponges for the market, read “Com-
mercial and Other Sponges” by Hyatt (No. III. in
“Guides for Science Teaching”).
180 PRACTICAL ZOOLOGY.
REVIEW OF ALL THE ANIMALS STUDIED.
1. How many different plans of structure have been shown by the
animals thus far examined ?
2. How many different ways of eating, and how do the digestive
organs differ?
3. What different arrangements for the circulation of the blood?
4. “Compare the various methods of breathing.
5. In what ways do animals effect motion and locomotion?
6
Describe the different sorts of organs of feeling, seeing, hearing,
smelling, and tasting.
7. Describe the methods of producing sounds.
8. What different kinds of coats do animals wear?
9. What weapons of attack and defence do they carry?
10. What different kinds of skeletons?
11. How many kinds of animals are native to your neighborhood?
12. The animals of a given region constitute its fauna. Thus, the
faune of North and South America are unlike; and North
America may be divided into regions having more or less dis-
tinct faune.
13. What characters are common to all the animals you have studied?
14. What is an animal?
BRANCHES OF THE ANIMAL KINGDOM.
(Packard.)
Vertebrata: Mammals, Birds, Reptiles, Batrachians, Fishes, ete.
Arthropoda: Crustaceans and Insects, Spiders, Myriapods, etc.
Mollusca: Bivalves, Snails, Cuttle-fishes, ete.
Vermes: Worms.
Echinodermata: Crinoids, Starfishes, Sea-urchins, etc.
et Fe ee
st at Sin aS
BOOKS OF REFERENCE. 181
3. Coelenterata: Hydroids, Jelly-fishes, Polyps, ete.
2. Porifera: Sponges.
1,
Protozoa: Amoeba, Paramcecium, Vorticella, ete.
BOOKS OF REFERENCE FOR THE ZOOLOGICAL
LABORATORY.
Of the following books, Nos. 1 to 9 are almost indis-
pensable. For general reference, at least, one of the first
three should be at hand, and every one of 4 to 9 gives
great aid in practical work.
1.
2.
3.
4,
Text-Book or ZodLocy. Claus & Sedgwick. Macmillan & Co.
2 vols. $8.00.
Zobiocy. Packard. Henry Holt & Co. $3.00.
Trext-Boox or ZoéLtocy. Nicholson. D. Appleton &Co. $1.50.
HaAnpBook OF INVERTEBRATE ZodLoGy. Brooks. Cassino.
$3.00.
PracticaAL Brotogy. Huxley & Martin. Macmillan & Co.
$1.50.
PracticaL Puysrotogy. Foster & Langley. Macmillan & Co.
$2.00.
Guipes ror Scrence Treacuinc. Hyatt and others. D. C.
Heath & Co. 10-40 cts. each.
First Boox or ZoéLocy. Morse. D. Appleton & Co. $1.00.
Zoébtomy. Parker. Macmillan & Co. $2.25.
Tue Crayrisn. Huxley. D. Appleton & Co. $1.75.
ANATOMY OF VERTEBRATED AND INVERTEBRATED ANIMALS.
Huxley. 2 vols. D. Appleton & Co. $2.50 each.
Guipe To THE Stupy or Insects. Packard. Henry Holt &
Co. $5.00.
Insects Insurrous TO VHGETATION. Harris. Cassino. $6.00,
182
14.
15.
16.
17.
18.
19.
20.
21.
22.
23.
24.
25.
26.
27.
28,
29.
30.
PRACTICAL ZOOLOGY.
Insects Insurious To Fruits. Saunders. J. B. Lippincott
& Co. $3.00.
VEGETABLE Movutp AND EArtHworms. Darwin. D. Apple-
ton & Co. $1.50.
Tue Naturatist’s Assistant. Kingsley. Cassino. $1.50.
CoMPARATIVE Zo6Loey. Orton. Harpers. $1.80.
SeasipE Strupres in Naturat History. Mrs. E. C. and
Alexander Agassiz. Houghton, Mifflin, & Co. $3.00.
SPIDERS, THEIR STRUCTURE AND Hasits. Emerton. Cas-
sino. $1.50.
Lire ON THE SEASHORE. Emerton. Cassino. $1.50.
MANUAL OF THE VERTEBRATES. Jordan.