JANUARY & FEBRUARY, 1998 US ISSN 0013-872X NO. 1 ENTOMOLOGICAL NEWS Dispersal & range expansion of introduced sand wasp, Oxybelus bipunctatus (Hymenoptera: Sphecidae), in northeastern North America Frank E. Kurczewski 1 Relationship of cell depth & soil moisture in Oxybelus bipunctatus (Hymenoptera: Sphecidae) F.E. Kurczewski, D.L. Wochadlo 7 Adventive lady beetles (Coleoptera: Coccinellidae) in eastern Nova Scotia, Canada D.B. McCorquodale 15 Camelobaetidius variabilis (Ephemeroptera: Baetidae), a new species from Texas, Oklahoma and Mexico N.A. Wiersema 21 Newly reported and little known mayflies (Ephemerop- tera) of Texas N.A. Wiersema 27 Redescription of Nerthra praecipua (Heteroptera: Gelastocoridae) from Chile J.T. Polhemus, T. Cekalovic K. 33 A new species of Symphitoneuria (Trichoptera: Lepto- ceridae) from Sabah, Malaysia T. Andersen, J. Huisman 37 A new species of Tomocerus (5.5.) (Collembola: Tomocerinae) from China Y-T. Ma, K.A. Christiansen 47 Tomocerus spinulus, (Collembola: Entomobryidae), a new species of Chinese springtail J- X. Chen, K.A. Christiansen 51 Species distinction in abdominal pigmentation patterns between females of Drosophila melanogaster and D. simiila us (Diptera: Drosophilidae), from a Spanish population K. Th. Eisses, M. Santos 56 Synonymic notes on some of Thomson's New World Dolichopodidae (Diptera) Daniel J. Bickel 61 Dry weight of fresh and preserved spiders (Ara- neida: Labidognatha) R.L. Edwards, W.L. Gabriel 66 Observations of interactive behavior in Parandra glabra (Coleoptera: Cerambycidae) Steven W. Lingafelter 75 SCIENTIFIC NOTES: Euhrychiopsis lecontei (Coleoptera: Curculionidae), a new state report for Indiana R.D. Waltz, G.M. White, R.W. Scriballo 6 Recent interception of live khapra beetle, Trogoderma granarium (Coleoptera: Dermestidae), at Port of Baltimore, MD M.L. Zimmerman, J.A. Barron 20 Unusual occurrence of caddisflies (Trichoptera: Phryganeidae) in a Penn. population of purple pitcher plant, Sarracenia purpurea R. Hamilton IV, R.L. Petersen, R.M. Duffield 36 CURTIS W. SABROSKY 60 THE AMERICAN ENTOMOLOGICAL SOCIETY ENTOMOLOGICAL NEWS is published bi-monthly except July-August by The American Entomological Society at the Academy of Natural Sciences, 1900 Benjamin Franklin Parkway, Philadelphia, Pa., 19103-1195. U.S.A. The American Entomological Society holds regular membership meetings on the fourth Wednesday in October, November, February. March, and April. The November, February and April meetings are held at the Academy of Natural Sciences in Philadelphia, Pa. The October and March meetings are held at the Department of Entomology, University of Delaware, Newark, Delaware. Society Members who reside outside the local eastern Pennsylvania, southern New Jersey, and Delaware area are urged to attend society meetings whenever they may be in the vicinity. 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Papers on applied, economic and regulatory entomology and on toxicology and related subjects will be considered only if they also make a major contribution in one of the aforementioned fields. (Continued on inside of back cover) Postmaster: // undeli verable , please send form 3579 to Howard P. Boyd, 232 Oak Shade Road, Tabernacle Twp., Vincentown, New Jersey 08088, U.SA. SECOND CLASS POSTAGE PAID AT VINCENTOWN, NEW JERSEY, 08088, U.S.A. Vol. 109, No. 1, January & February, 1998 DISPERSAL AND RANGE EXPANSION OF AN INTRODUCED SAND WASP, OXYBELUS BIPUNCTATUS (HYMENOPTERA: SPHECIDAE), IN NORTHEASTERN NORTH AMERICA1 Frank E. Kurczewski^ ABSTRACT: Range expansion of an introduced sphecid, Oxybelus bipunctatus, is traced through the northeastern United States and southeastern Canada. This species apparently is displacing O. uniglumis quadrinotatus, a species with similar behavior and ecology, in abundance as it moves northward through the Great Lakes Region, St. Lawrence River Valley, and Maritime Provinces to become the most common Oxybelus in small inland sandy areas. Its southward dispersal probably is limited by climatic, ecological and edaphic factors such as high summer and warm winter temperatures, thermal soil temperature regime, and absence of appropriate sandy soils. Oxybelus bipunctatus Olivier is a small digger wasp that usually excavates 1 - and 2-celled nests in sandy soil, impales adult flies on its sting, removes the temporary nest closure with the fly still impaled, and stocks its cells mostly with several male Brachycera and/or Cyclorrapha (Peckham et al. 1973; Krombein 1979; Kurczewski 1996). This wide ranging Holarctic species evi- dently was accidentally introduced into the northeastern United States from Europe before 1935 (Pate 1943; Krombein 1979), perhaps in ship ballast or molding sand deposited dockside. Based upon insect museum specimens, the earliest dates of its occurrence in North America are 1935 from Essex County, New Jersey and Hampden County, Massachusetts, and 1936 from Suffolk County, New York (Table 1). By the 1940's O. bipunctatus was firmly estab- lished in the Middle Atlantic Region and New England (Pate 1945, pers. obs.). Since that time the range of this species has been expanding primarily north- ward, most recently into western upper Michigan, central Wisconsin, and Nova Scotia. Within 10 years of its introduction into the U. S., the range of 0. bipunctatus extended to Washington, D. C., a distance of over 300 km from its first North American collection locality (Fig. 1). In only 20 years' time, this species dis- persed to North Bay, Ontario and central lower Michigan, a distance of 660- 900 km. In less than 50 years, O. bipunctatus moved as far west as western upper Michigan and central Wisconsin, a distance of 1,250 km. On a smaller scale, this species dispersed from Ottawa to North Bay, Ontario, probably across sandy sections of the Ottawa-Mattawa River Valley,a distance of about 300 km, 1 Received March 21, 1997. Accepted June 29, 1997. 2 Environmental and Forest Biology, State University of New York College of Environmental Science and Forestry, Syracuse, New York 13210-2778. ENT. NEWS 109(1 ): 1-6, January & February, 1998 ~ ENTOMOLOGICAL NEWS in nine years (1947-1956). Oxybelus bipunctatus moved from Clinton County to Ogemaw County, Michigan, a distance of about 1 70 km, in five years ( 1 954- 1959). Using these durations and distances as gages of dispersal, the rate of movement of O. bipunctatus amounted to approximately 30-45 km per year assuming the species was not transported to these regions by some means other than its own flight. This is a remarkably rapid rate of dispersal for a species limited to short flights by its small size and stocky build, although the possibil- ity of wind carriage should not be overlooked. In only a half century, this species dispersed through New England to the Maritime Provinces (New Brunswick, Nova Scotia) and St. Lawrence River Valley (southern Quebec), across the Great Lakes Region (upstate New York, southern Ontario, northwestern Pennsylvania, northern Ohio, upper and lower Michigan, central Wisconsin), and down the Atlantic Coastal Plain to Mary- land, District of Columbia, and northern Virginia (Table 1 , Fig. 1 ). The lack of specimens of O. bipunctatus from southern Ohio and southern Virginia indicate that its southward dispersal may be climatically and ecologically limited by factors such as high summer and warm winter temperatures and/or thermal soil temperature regime (USDA 1984), or it may simply reflect inappropriate habi- tat (soils) for this species in these areas. Despite its adventive status, O. bipunctatus is one of the most common sphecids in small inland sandy areas in the northeast (Kurczewski and Harris 1968; Kurczewski and Acciavatti 1990). O'Brien (1996 pers. comm.) found it to be the most numerous species of Oxybelus in Malaise traps at the Huron Mountain Club, Marquette County, Michigan beginning in the early 1980's (Fig. 1). McCorquodale (1997 pers. comm.) indicated that this species is more com- mon than any other species of Oxybelus in sandy areas on Cape Breton Island, Nova Scotia. Its abundance in these regions probably can be attributed to cold hardiness, long flight season, relatively short generation time, wide prey selec- tion (Peckham et al. 1973; Krombein 1979; Kurczewski 1996), scarcity of natural enemies (Spofford and Kurczewski 1990), and ubiquity in nesting habitat. Oxybelus bipunctatus nests opportunistically in such varied places as flower pots, children's sand boxes, temporary highway sand piles, and the sand bases of swimming pools. Because of its small size and capability to nest shallowly in soil, it is an excellent candidate for transport by human means from one place to another including transoceanic portage. Although quantitative data are unavailable, O. bipunctatus apparently is supplanting O. uniglumis quadrinotatus in abundance in sandy and gravelly areas as it disperses northward through the Great Lakes Region, St. Lawrence River Valley, and Maritime Provinces. The displacement in upstate New York began rather suddenly during the 1950's (Evans 1960 pers. comm.). Oxybelus bipunctatus appeared in the Ithaca, New York area as early as 1937 (Pate 1943), possibly transported there by human means, and by the 1960's it was flourish- Vol. 109, No. 1, January & February, 1998 Fig. 1. Regional dispersal of Oxybelus bipunctatus by year of collection. Arrows indicate prob- able dispersal pathways. ENTOMOLOGICAL NEWS ing in small inland sandy areas of upstate New York (Kurczewski and Harris 1968; Peckham et al. 1973; Kurczewski and Acciavatti 1990). Oxybelus bipunctatus is now the most abundant member of the genus in northeastern United States and southeastern Canada (pers. obs.)- Some nesting aggregations Table 1. Pre-1955 dates and localities of collection of Oxybelus bipunctatus arranged in chrono- logical order. DATE LOCALITY SOURCE July 4, 1935 Bloomfield, Essex County, NJ AMNH July 29, 1935 Holland, Hampden County, MA MCZ June?, 1936 Half Hollows, Suffolk County, NY Pate 1943 July 11, 1937 Reading, Middlesex County, MA MCZ August 2, 1937 Durham, Middlesex County, CT MCZ August 2, 1937 Ithaca, Tompkins County, NY Pate 1943 August 31, 1939 Keamy, Hudson County, NJ AMNH July 2, 1944 Princeton, Mercer County, NJ USNM May 4, 1945 Stinson Lake, Grafton County, NH cu July 23-25, 1945 Washington, DC USNM July 24, 1945 Washington, DC Pate 1945 June 23, 1946 Princeton, Mercer County, NJ USNM [F12], 1947 Princeton, Mercer County, NJ USNM June 11 -July 15, 1947 Washington, DC USNM, MSU June 22-29, 1947 Arlington, Fairfax County, VA USNM June 27, 1947 Silver Springs, Montgomery County, MD USNM, UCD July 3, 1947 Ottawa, Municipality of Ottawa-Carleton, ONT CNC June 15, 1949 East Hartford, Hartford County, CT CU July 14, 1951 Parke Reserve, Kamouraska County, QUE CNC July 22, 1952 Rensselaerville, Albany County, NY USNM June 7- August 20, 1953 Ithaca, Tompkins County, NY MCZ, USNM July 26, 1953 Guelph, Wellington County, ONT UG June 25, 1954 Guelph, Wellington County, ONT UG JulyS, 1954 Clinton County, MI MSU July 9, 1954 Orangeville, Dufferin County, ONT UG Septembers, 1954 Ithaca, Tompkins County, NY MCZ Abbreviations for insect museums are as follows: AMNH, The American Museum of Natural History; CNC, Canadian National Collection; CU, Cornell University; MCZ, Museum of Com- parative Zoology, Harvard University; MSU, Michigan State University; UCD, University of California-Davis;UG, University of Guelph; and, USNM, United States National Museum. Vol. 109, No. 1, January & February, 1998 of this species number several hundred individuals (Peckham et al. 1973; Kurczewski and Acciavatti 1990). Why is O. bipunctatus displacing O. uniglumis quadrinotatus in the north- eastern United States and southeastern Canada? Although the two species seemingly have identical nesting behaviors and similar ecological requirements (Peckham et al. 1973), O. uniglumis quadrinotatus is almost twice the size of O. bipunctatus. Because of this size difference the two species might be ex- pected to prey upon different families, genera and species of flies. However, they capture some of the same groups of prey with O. uniglumis quadrinotatus taking a slightly greater proportion of Cyclorrapha and fewer Brachycera than O. bipunctatus. Within the suborder Cyclorrapha, there is some predation on the same species of flies (Peckham et al. 1973; Krombein 1979). Oxybelus uniglumis quadrinotatus never captures Nematocera while O. bipunctatus oc- casionally preys upon mosquitos, midges, and blackflies (Evans 1963; Kurczewski 1996). A broader spectrum of prey, smaller size and inconspicu- ousness, potentially fewer predators, and a lower rate of cleptoparasitism (Spofford and Kurczewski 1990) are some reasons why O. bipunctatus is more successful and, therefore, more numerous than O. uniglumis quadrinotatus in many sandy areas of the northeastern U. S. and southeastern Canada. ACKNOWLEDGMENTS The following persons provided specimens, collection data, and/or abundance information for O. bipunctatus: Bert Finnamore, Alberta Provincial Museum; Rick Hoebeke, Cornell Univer- sity; Michael Kelly, Museum of Comparative Zoology, Harvard University; Lynn Kimsey, Uni- versity of California-Davis; Steve Krauth, University of Wisconsin-Madison; Lubomir Masner, Canadian National Collection; David McCorquodale, Guelph University, Nova Scotia Natural History Museum, and University College of Cape Breton; Ron McGinley and Arnold Menke, United States National Museum; Mark O'Brien, Museum of Zoology, The University of Michi- gan; Mark Snethurst, The American Museum of Natural History; Andrey Sharkov, The Ohio State University; Jeff Skevington, Pinery Provincial Park; Don Snitgen, Northern Michigan Uni- versity; and John Wilterding, Michigan State University. I am grateful to David McCorquodale and two anonymous reviewers for critically reviewing the manuscript. Julie Cushine-Rigg final- ized Figure 1 . LITERATURE CITED Evans, H. E. 1963. Wasp Farm. Natural History Press, Garden City, N. Y. 178pp. Krombein, K. V. 1979. Superfamily Sphecoidea. In: K. V. Krombein, P. D. Hurd, Jr., D. R. Smith, and B. D. Burks, eds. Catalog of Hymenoptera in America North of Mexico, vol 2, pp. 1573-1740. Smithson. Inst. Press, Washington, D. C. Kurczewski, F. E. 1996. Variation in nesting behavior of Oxybelus bipunctatus and O. emarginatus, with notes on other species (Hymenoptera: Sphecidae). Mem. Entomol. Soc. Wash. Contrib. Hym. , pp. 82-88. Kurczewski, F. E. and R. E. Acciavatti. 1990. Late summer-fall solitary wasp fauna of central New York (Hymenoptera: Tiphiidae, Pompilidae, Sphecidae). Great Lakes Entomol. 23: 57-64. ENTOMOLOGICAL NEWS Kurczewski, F. E. and B. J. Harris. 1968. The relative abundance of two digger wasps, Oxybelus bipunctatus and Tachysphex terminatus, and their associates, in a sand pit in central New York. J. N. Y. Entomol. Soc. 76: 81-83. Pate,V. S. L. 1943. On some Holarctic sphecoid wasps (Aculeata, Hymenoptera). Bull. Brook- lyn Entomol. Soc. 38: 14-16. Pate, V. S. L. 1945. On two species of Oxybelus at Washington, D. C. (Hymenoptera, Sphecidae). Bull. Brooklyn Entomol. Soc. 40: 165. Peckham, D. J., F. E. Kurczewski, and D. B. Peckham. 1973. Nesting behavior of Nearctic species of Oxybelus (Hymenoptera: Sphecidae). Ann. Entomol. Soc. Amer. 66: 647-661. Spofford, M. G. and F. E. Kurczewski. 1990. Comparative larvipositional behaviours and cleptoparasitic frequencies of Nearctic species of Miltogrammini (Diptera: Sarcophagidae). J. Nat. Hist. 24: 731-755. United States Department of Agriculture. 1984. General soil map of the northeastern United States. SCIENTIFIC NOTE EUHRYCHIOPSIS LECONTEI (COLEOPTERA: CURCULIONIDAE): A NEW STATE REPORT FOR INDIANA1 R.D. Waltz2, G.M. White3, R.W. Scribailo4 The range of the native North American aquatic weevil species Euhrychiopsis lecontei (Dietz) is poorly documented (Sheldon and O'Bryan 1996) although it has been reported from coast to coast in various northern and central states including Iowa, Michigan, Wisconsin, Alberta, Brit- ish Columbia, and Saskatchewan (O'Brien and Wibmer 1982), Washington (Creed and Sheldon 1994), Minnesota (Newman and Maher 1995), and Connecticut, Illinois, Massachusetts, New York and Vermont (Sheldon and O'Bryan 1996). Because this species is recognized as a poten- tially effective endemic biological control agent of watermilfoils (Haloragaceae: Myriophyllum spp.) (Creed and Sheldon 1993; Sheldon and O'Bryan 1996), records of its distribution are of special interest to those managing lakes and to those who have an interest in the documentation of insect faunae within regional or politically delineated contexts. Euhrychiopsis lecontei has not been reported previously from Indiana (above citations; Blatchley and Leng 1916; Downie and Amett 1996). Herein, we report the first known record of this species in Indiana. Voucher specimens were taken from: In: LaPorte Co., Saugany Lake, May 16, 1997, G.M. White, collected in association with Eurasian watermilfoil (Myriophyllum spicatum L.). Voucher specimens have been deposited at Purdue University Entomological Re- search Collections, West Lafayette, Indiana. Adults were collected and numerous eggs were observed on the stem apices of Eurasian (continued on page 14) 1 Received June 7, 1997. Accepted July 4, 1997. 2 IDNR, Division of Entomology and Plant Pathology, 402 West Washington, Room W-290, Indianapolis, IN 46204. 3 IDNR, Division of Soil Conservation, 402 West Washington, Room W-265, Indianapolis, IN 46204. 4 Biological Sciences and Chemistry Section, Purdue University - North Central, 1401 South Hwy US 421, Westville, IN 46391. Vol. 109, No. 1, January & February, 1998 RELATIONSHIP OF CELL DEPTH AND SOIL MOISTURE IN OXYBELUS BIPUNCTATUS (HYMENOPTERA: SPHECIDAE)1 Frank E. Kurczewski, Donna L. Wochadlo^ ABSTRACT: Nests of Oxybelus bipunctatus were excavated and cell depth measured in control and experimentally watered plots in a recently bulldozed sandy field in central New York in June 1987. There was a trend for cell depth to be inversely related to soil moisture under natural condi- tions and in artificially watered replicates. Soil moisture content is extremely critical for the survival of the immature stages of ground inhabiting solitary wasps. Too little moisture in the soil results in dessication of the paralyzed prey in the cells and the eventual death of the wasp egg or larva. Too much soil moisture increases the likelihood of mold development in the cell and this, too, can destroy the cell contents and wasp egg or larva. A delicate balance in the amount of soil moisture is necessary for the immature stages of the wasps to develop properly. Many physical and biotic factors influence soil moisture content and regulate cell environment. Cell depth and amount of precipitation are two such factors that can be readily measured without elaborate or expensive equipment. The present study is an attempt to investigate the relationship between the amount of rainfall, depth of soil mois- ture line, and cell depth in a ground-nesting sphecid, Oxybelus bipunctatus Olivier. Oxybelus bipunctatus, a species adventive from Europe (Pate 1943, 1945; Krombein 1979; Kurczewski 1998), is one of the most common digger wasps in the northeastern U. S. (Kurczewski and Harris 1968; Kurczewski and Acciavatti 1990). Ubiquity in nesting habitat, a long and continuous flight pe- riod involving two or three generations per year, and high nest density make this species an excellent subject for studying nesting behavior in a solitary wasp. Peckham et al. (1973) investigated the behavior of O. bipunctatus over three years. Although they carefully measured nest dimensions, these authors did not examine the relationship between cell depth and soil moisture content. Kurczewski ( 1 996) noted the difference in cell depth of O. bipunctatus between localities in central New York and northwestern Pennsylvania but he, too, did not account for soil moisture level. In a 15 weeks-long investigation of O. bipunctatus at a site in upstate New York, the same author (in prep.) observed weekly fluctuation in cell depth that seemed to be linked to soil moisture level 1 Received March 20, 1997. Accepted June 27, 1997. 2 Environmental and Forest Biology, State University of New York College of Environmental Science and Forestry, Syracuse, New York 13210-2778. ENT. NEWS 109(1): 7-14, January & February, 1998 ENTOMOLOGICAL NEWS as influenced by the amount of rainfall. The present study is an extension of this finding. Using measurements of rainfall, soil moisture line, and cell depth, we tried to corroborate Kurczewski's (in prep.) results. By artificially adding water to designated sand plots, we attempted to demonstrate a relationship be- tween soil moisture content and cell depth. MATERIALS AND METHODS A recently bulldozed sandy field in northwestern Madison County, New York, 5.6 km NW of Chittenango, a site used previously by Spofford et al. (1986) for a study on the cleptoparasites of another sphecid, Tachysphex terminatus (Smith), was selected as the study area. Natural conditions were monitored during 5-15 June 1987. This involved collecting and measuring the amount of rainfall from the previous day(s) and/or night(s) in mm, taking air (shade), sand surface and cell depth temperature in degrees C, and noting any change or disturbance to the area. Morning activity of individual wasps was correlated with air and cell depth temperature. Females were observed digging burrows in the morning on 5, 6, 10, 1 1 , and 14 June 1987, and their nest entrances were marked with color-coded tooth- picks after completion of the temporary nest closures. (The wasps themselves were too small to color-code). The cells were excavated the following morning or afternoon after the wasps completed their final closures, measurements were taken, and new nests were found and marked for the next day, weather permitting. The relationship between cell depth and soil moisture line, a readily visible band of dark moist sand underlying a layer of light colored dry sand (Kurczewski in prep.), was examined for 35 of the cells. Depths of soil moisure line and cell to its bottom were measured with a metric ruler and recorded in mm. Depths of 53 other cells were also measured and recorded. Each excavation was then levelled flush with the surrounding sand and lightly packed down to encourage renesting. Two areas of bare sand of equal size were selected and each area divided into control and experimental plots (Fig. 1). The selection of these areas was based upon local topography, lack of vegetation, and density of nesting wasps. One area was divided in half by a north-south line into 240 X 280 cm plots and labelled NC (North Control) and NE (North Experimental). The other area was divided in half by an east-west line into 480 X 140 cm plots and labelled SC (South Control) and SE (South Experimental). The direction of division into control and experimental plots coincided with slight slopes in the respective areas and was an attempt to eliminate any variables. On June 16, we artificially watered the experimental plots to achieve a soil moisture level sufficiently higher than that in the control plots yet not inhibit Vol. 109, No. 1, January & February, 1998 wasp excavation or create soil surface run-off. Using a 1 .5 gal sprinkling can, we evenly applied 10 gallons of water to each experimental area in rows 20 cm wide beginning at 0920 hrs (EOT). Prior to watering, five 50 ml plastic gradu- ated cylinders were set into the sand in each experimental plot, four of them each 30 cm in from a corner and one directly in the center of the plot (Fig. 1 ). 240cm 240cm NE NC O 0 n 12 9 8 7 6 13 10 2 C O 15 1 5 14 L O O 4 00 OJ 3 96'27n a*. , ] 3 5 Q\ 6 2 8 ^>\ O SE o F o o o O % 1 — sc £ o o 480cm Fig. 1 . Design of control (NC, SC) and experimental (NE, SE) plots in Oxybelus bipunctatus study area. Circles represent graduated cylinders set into sand to collect water. Numbers in NE and NC are locations of nest entrances sequentially marked on 24 June 1987. 10 ENTOMOLOGICAL NEWS The relative uniformity of our watering technique was reflected in the amount of water collected by the graduated cylinders: (NE), 4.0, 4.5, 6.0 (central), 5.0, 5.0 ml; and, (SE), 5.0, 4.0, 4.0 (central), 4.5, 5.0 ml. On 24 June, we applied 20 gal of water to the NE plot utilizing the same technique, except the application was made between 2000-2100 hrs. We felt that an application at dusk would substantially decrease water evaporation from the sand surface and, by doubling the amount of water put into the soil, the wasps might dig significantly shallower nests in this plot. After the application, each of the five plastic graduated cylinders placed in the plot contained 7.5-8.0 ml of water. We intended to similarly water the SE plot the same evening, but it had been severely disturbed the previous night by a truck carrying a bulldozer and was abandoned for further use. RESULTS Temperature and rainfall. Only five of the 1 1 days (see above) set aside to study O. bipunctatus under natural conditions were suitable for wasp nest- ing. During these days, air temperatures averaged 22.8° C (18-25° C, n=5) and soil temperatures at cell depth averaged 26.7° C (23-31° C, n=5) at 1100 hrs (EOT). The six other days set aside for study under natural conditions were non-conducive for wasp nesting because of cool temperatures, cloud cover, and rain. It rained sporadically in three and heavily during two of the days (Fig. 2). 50 45 40 35 E 30 £_ I25 S. 20 15 10 5 0 • Rainfall D Mean cell depth 10 Day (June 1987) 11 14 50 49 48 47 ? 46 ~ Q. 45 "S Fig. 2. Amount of rainfall and mean cell depth in Oxybelus bipunctatus study area, 5- 1 4 June Amount of rainfall is a measurement from previous day(s) and/or night(s). 44 43 42 41 40 1987. Vol. 109, No. 1, January & February, 1998 The average air and mean cell depth temperatures on these mornings at 1100 hrs (EOT) were only 17.2° C (15-18° C, n=6) and 21.1° C (18-22° C, n=6), respectively. Soil moisture line. The soil moisture line was indistinguishable from the overlying sand on days when it rained heavily. On non-rainy days, this line was clearly visible and cells were invariably excavated below it in moist sand. There was an imperfect positive correlation between soil moisture line and cell depth (Fig. 3). In some nests, the soil moisture line to cell depth value was more disparate than in other nests (Fig. 3). Cell depth. Females excavated fewer and deeper nests in drier soil. They dug clumped and shallower nests in moister soil. Nests excavated in neither dry nor wet sand were intermediate in depth and more evenly distributed than nests in wet or dry sand (Figs. 1, 2). Mean cell depth and amount of rainfall from the previous day(s) and/or night(s) were inversely related (Fig. 2). In early to mid-June, slight increases or decreases in mean cell depth followed corresponding decreases or increases, respectively, in the amount of rainfall (Fig. 2). For example, shallowness in mean cell depth on 10, 11 and 14 June was probably linked to a substantial amount of rainfall and increased soil mois- ture content on 10-11 June. Nests excavated during 5-14 June under natural conditions had, on average, shallower cells (x=44.4±5.38 mm; n=88; Fig. 2), albeit not significantly so, than control plot nests dug on 1 6-24 June (x=47.3±6.29 mm; n=67; Table 1). This difference probably was due to the different amounts Table 1 . Cell depth of Oxybelus bipunctatus, control and experimental plots, 16-24 June 1987. Site Information Cell Depth (mm) Date Plot n Range Mean 6/16/87 NC 10 38-59 46.6+5.99 6/16/87 SC 10 38-58 47.8+6.23 6/17/87 SC 17 38-56 45.8+5.54 6/17/87 SE 10 26-45 37.8+8.16 6/17/87 NC 15 35-55 48.1+5.79 6/17/87 NE 11 37-65 45.2+8.22 6/24/87 NC 15 34-63 48.2+7.88 6/24/87 NE 12 39-61 47.6+6.77 12 ENTOMOLOGICAL NEWS (uiui) o (D m LO o LO in ^ o ^ Lo CO o CO m CN 10 CO 5X^ S< 0 . :X- i? O V/ LO ~r nr o 10 (LULU) au;~| ajnjsio|/yj |;os O CO tn CNJ 0 I "1 z LO ^ - LO Si ~ cfl 4> ts e o C S* . C/5 [--. rt 00 " <* E si ;* «••? * y *-• C S Si c ^ § - 2 -a a M S S C «9 a u .S-=5 *.S 3| ^ .a -o &> ca II .a s» f,« 4J Q, T3 IU — "O "o s O l) •o « II II c/3 a> 11 11 §1 ? eo 5 1 U U O on C 8 &. Fig. 3. Di surface, X Vol. 109, No. 1 , January & February, 1998 13 of rainfall during these times of the month (5-14 June, 54.5 mm; 16-24 June, 45.7 mm). Mean cell depth for 16 June NC and SC nests was similar (t=0.0793, df=18, cc>0.20) (Table 1). On 17 June, wasps excavated significantly deeper cells in SC than in SE (t=3.0415, df=25; oxO.Ol) (Table 1). There was no significant difference in mean cell depth between two samples each comparing NC and NE nests (June 17, t=l .0572, df=24, a>0.20; June 24, t=0.0397, df=25, a>0.20), although the wasps did excavate slightly shallower cells in both NE plots (Table 1). All nests (n=12) in NE excavated on 24 June were clustered downslope in the SW corner in moist sand (Fig. 1). DISCUSSION Few studies on fossorial sphecid wasps considered the influence of relative soil moisture on cell depth. Moisture gradient influenced cell depth throughout the nesting season in Microbembex nigrifrons (Provancher). Deeper cells were dug in drier and shallower cells in wetter sand (Alcock and Ryan 1973). Soil moisture level was related to cell depth in Ammophila harti (Fernald). Cells excavated in drier sand remained deeper than those dug in wetter sand regard- less of generation of wasp (Hager and Kurczewski 1986). Cell depth and, less so, soil moisture line closely followed an increase or decrease in relative soil moisture, as governed by the amount of rainfall, throughout the nesting season in Oxybelus bipunctatus at one locality in central New York (Kurczewski in prep.). In the present study, both soil moisture line and cell depth were inversely related to the amount of rainfall. There was, however, an imperfect positive correlation between soil moisture line and cell depth with some values being more disparate than others, even in perfectly flat sand. Regardless of possible flaws in the experimental design, shallower cells were usually excavated in moister and deeper cells in drier sand. However, only one control versus experi- mental plot sample was conclusive. In this comparison, SE cells in artificially watered sand were excavated at significantly shallower depths than SC cells. ACKNOWLEDGMENTS We thank M. G. Spofford for assisting with field work on the last day of the study. We are grateful to D. J. Peckham for reading the manuscript critically. Use of a college vehicle was made possible through an NYS-UUP Faculty Development Award to the senior author. LITERATURE CITED Alcock, J. and A. F. Ryan. 1973. The behavior of Microbembex nigrifrons (Hymenoptera: Sphecidae). Pan-Pac. Entomol. 49: 144-148. Hager, B. J. and F. E. Kurczewski. 1986. Nesting behavior of Ammophila Hani (Fernald) (Hymenoptera: Sphecidae). Amer. Midi. Nat. 116: 7-24. Krombein, K. V. 1979. Superfamily Sphecoidea. In: K. V. Krombein, P. D. Hurd, Jr., D. R. Smith, and B. D. Burks, eds. Catalog of Hymenoptera in America North of Mexico, vol. 2, pp. 1 573- 1740. Smithson. Inst. Press, Washington, D. C. 14 ENTOMOLOGICAL NEWS Kurczewski, F. E. 1 996. Variation in nesting behavior of Oxybelus bipunctatus and O. emarginatus, with notes on other species (Hymenoptera: Sphecidae). Mem. Entomol. Soc. Wash. Contrib. Hymen, pp. 82-88. Kurczewski, F. E. 1998. Dispersal and range expansion of an introduced sand wasp, Oxybelus bipunctatus, in northeastern North America (Hymenoptera: Sphecidae). Entomol. News 109: 1-6. Kurczewski, F. E. and R. E. Acciavatti. 1990. Late summer- fall solitary wasp fauna of central New York (Hymenoptera: TiphiidaePompilidae, Sphecidae).Great Lakes Entomol. 23: 57-64. Kurczewski, F. E. and B. J. Harris. 1968. The relative abundance of two digger wasps, Oxybelus bipunctatus and Tachysphex terminatus, and their associates, in a sand pit in central New York. J. N. Y. Entomol. Soc. 76: 81-83. Pate, V. S. L. 1 943. On some Holarctic sphecoid wasps (Aculeata, Hymenoptera). Bull. Brooklyn Entomol. Soc. 38: 14-16. Pate, V. S. L. 1945. On two species of Oxybelus at Washington, D. C. (Hymenoptera, Sphecidae). Bull. Brooklyn Entomol. Soc. 40: 165. Peckham, D. J., F. E. Kurczewski, and D. B. Peckham. 1973. Nesting behavior of Nearctic species of Oxybelus (Hymenoptera: Sphecidae). Ann. Entomol. Soc. Amer. 66: 647-661. SpofTord, M. G., F. E. Kurczewski, and D. J. Peckham. 1986. Cleptoparasitism of Tachysphex terminatus (Hymenoptera: Sphecidae) by three species of Miltogrammini (Diptera: Sarcopha- gidae). Ann. Entomol. Soc. Amer. 79: 350-358. (continued from page 6) watermilfoil. Based on the observation of adult activity in May and the presence of numerous eggs, we believe E. lecontei is established in Indiana and we anticipate that the biology of E. lecontei in Indiana will be very similar to that reported by Sheldon and O'Bryan (1996). ACKNOWLEDGMENTS We thank C.W. O'Brien and Peter Kovarik, Florida A&M, for providing comparative mate- rial to confirm our identification of the species, and thank David Barnes, Mitchell Alix, and Bob Jankowski, Purdue University North Central, for assisting in making collections of this species. LITERATURE CITED Blatchley, W.S. and C.W. Leng. 1916. Rhynchophora or Weevils of North Eastern America. The Nature Publ. Co., Indianapolis, IN. 682 pp., illus. Creed, R.P., Jr. and S.P. Sheldon. 1993. The effect of feeding by a North American weevil, v Euhrychiopsis lecontei, on Eurasian watermilfoil (Myriophyllum spicatum). Aquat. Bot. 45: 245-256. Creed, R.P., Jr. and S.P. Sheldon. 1994. Aquatic weevils (Coleoptera: Curculionidae) associ- ated with northern watermilfoil (Myriophyllum sibiricum) in Alberta Canada. Entomol. News 105:98-102. Downie, N.M. and R.H. Arnett. 1996. The Beetles of Northeastern North America, Volume II. Sandhill Crane Press, Gainesville. 1721 pp. Newman, R.M. and L.M. Maher. 1995. New records and distribution of aquatic insect herbi- vores of watermilfoils (Haloragaceae: Myriophyllum spp.) in Minnesota. Entomol. News 106: 6-12. O'Brien, C.W. and G.J. Wibmer. 1982. Annotated checklist of the weevils (Curculionidae sensu lato) of North America, Central America, and the West Indies. Mem. Am. Entomol. Inst. #34. Sheldon, S.P. and L.M. O'Bryan. 1996. Life history of the weevil Euhrychiopsis lecontei, a potential biological control agent of Eurasian watermilfoil. Entomol. News 107: 16-22. Vol. 1 09, No. 1 , January & February, 1 998 15 ADVENTIVE LADY BEETLES (COLEOPTERA: COCCINELLIDAE) IN EASTERN NOVA SCOTIA, CANADA1 D. B. McCorquodale2 ABSTRACT: Specimens in three small insect collections document the arrival of four species of adventive lady beetles in Nova Scotia. All four have experienced dramatic range expansions in eastern North America in the past 25 years. Coccinella septempunctata arrived by 1984 and by the 1990's was the most common coccinellid in disturbed habitats on Cape Breton Island, NS. The more recent arrivals in Nova Scotia of Propylea quaiuordecimpunctata (1990), Harmonia axyridis (1995) and Hippodamia variegata (1996) are reported. Several species of lady beetles have become established in North America after either accidental or intentional introductions in the past 25 years (Gordon 1985; Gordon and Vandenberg 1991). Populations of some of these intro- duced species have burgeoned to the extent they have the potential to change dramatically populations of both native and other introduced lady beetles (e.g. Tedders and Schaefer 1994; Wheeler and Hoebeke 1995). Coccinellids de- serve attention so that we can document which introduced species become established, the speed of range expansion and major changes in populations. Recently, Hoebeke and Wheeler (1996) recorded the range expansion of adventive lady beetles in the Maritime provinces of Canada through the sum- mer of 1995. Here I provide updated information from eastern Nova Scotia on three of the four species they reported, Propylea qualuordecimpunciala, Hippodamia variegata and Harmonia axyridis, and information on a species established earlier, Coccinella septempunctata. METHODS All specimens of Coccinellidae in the small insect collections at the Uni- versity College of Cape Breton, Sydney, Nova Scotia (UCCB), St. Francis Xavier University, Antigonish, Nova Scotia (STFXU), and the Nova Scotia Department of Natural Resources, Belmont, Nova Scotia (NSDNR) were ex- amined. Identifications were based on Gordon ( 1 985), Gordon and Vandenberg (1991), and comparison with specimens in the University of Guelph Collec- tion, Guelph, Ontario. Representative specimens will be deposited in the Ca- nadian National Collection, Ottawa, Ontario and/or the Nova Scotia Museum of Natural History, Halifax, Nova Scotia. 1 Received June 6, 1997. Accepted July 19, 1997. 2 Department of Behavioural and Life Sciences, University College of Cape Breton, Sydney, Nova Scotia B1P6L2. ENT. NEWS 109(1): 15-20, January & February, 1998 16 ENTOMOLOGICAL NEWS RESULTS Coccinella septempunctata L.: Since 1973 this species has spread over most of the continental United States and southern Canada (Gordon and Vandenberg 1991). Schaefer et al. (1987) reported it in Nova Scotia in 1985. Collections from mainland Nova Scotia in 1984 and Cape Breton Island in 1985 demonstrate that the species was widespread in the province by 1985. Additional Locality Records: Cumberland County, Chignecto, 5 June 1984, E. Georgeson, NSDNR; Cape Breton County, Sydney, 26 August 1985, S. Tynski, UCCB; Cape Breton County, Sydney, 16 May 1986, S. Tynski, UCCB Since 1990, students at the University College of Cape Breton have col- lected 171 specimens in the subfamily Coccinellinae. Collections are largely from disturbed or anthropogenic habitats in the Sydney-Glace Bay area of Cape Breton Island. About half of these specimens are Coccinella septempunctata (86 or 50%), while native species are the next four most fre- quently collected: Adalia bipunctata (24, 1 4%), C. trifasciata (19,11%), Anatis mail (10, 6%) and Psyllobora vigintiduopunctata (9, 5%). Propylea quatuordecimpunctata (L): This species was established in Que- bec by 1968, had spread to Maine by 1988 and since then to much of the northeastern United States (Day et al. 1994). There is a strong probability that an independent establishment occurred in lower New York State in the mid- late 1980's (Yanega 1996). Hoebeke and Wheeler (1996) provide the first report for Nova Scotia and record collections from Cape Breton Island in 1995. Collections in the summer of 1996 from Cape Breton and Antigonish Counties indicate that it is now established in eastern Nova Scotia. Additional Locality Records: Cape Breton County, Georges River, 8 June 1996, DB McCorquodale, UCCB; Cape Breton County, Sydney, UCCB Campus, 4 September 1996, S. Berrigan, UCCB; Cape Breton County, Sydney, 5 September 1996, T. Rideout, UCCB; Antigonish County, Pomquet, 05 June 1996, R. Lauff, STFXU; Antigonish County, Pomquet, 22 July 1996, R. Lauff, STFXU; Antigonish County, Pomquet, 21 August 1996, R. Lauff, STFXU; Antigonish County, Antigonish, 15 July 1996, R. Lauff, STFXU Propylea quatuordecimpunctata was established in Nova Scotia for sev- eral years prior to the first collections reported by Hoebeke and Wheeler ( 1 996). The Nova Scotia Department of Natural Resources collection has 35 speci- mens collected between 1990 and 1995, mostly from Colchester County, but also from Pictou and Halifax Counties. The earliest collections are from Debert, Colchester County in 1990. Nine more were collected in Debert in 1991 . The first collections from Pictou County were in 1994 and in Halifax County in 1995. Vol. 1 09, No. 1 , January & February, 1 998 17 Additional Locality Records: Colchester County, Debert, 13 June 1990, E. Georgeson, NSDNR; Colchester County, Debert, 16 June 1990, T.D. Smith, NSDNR; Colchester County, Debert, 19 June 1990, E. Georgeson, NSDNR; Pictou County, Lyons Brook, 4 July 1994, E. Georgeson, NSDNR; Halifax County, Peggy's Cove, 15 August 1995, J. Ogden, NSDNR Harmonia axyridis (Pallas): Many attempts to introduce this species in North America, including Nova Scotia, occurred between 1916 and 1983 (Gordon 1985; Hoebeke and Wheeler 1996). The first established population was recorded in Louisiana in 1988 (Chapin and Brou 1991). Since then there has been an explosive range expansion and population explosion in northeastern North America (see Kidd et al. 1 995), with the first records for Canada in 1 994 (Coderre et al. 1995; Marshall 1995). This range expansion has continued into the Maritimes with Hoebeke and Wheeler (1996) reporting it from three sites, including two in Nova Scotia (Lunenburg and Digby Counties). They did not record it from Cape Breton Island, where it has since been collected during the summers of 1995 and 1996. Additional Locality Records: Cape Breton County, Sydney, UCCB Campus, 19 September 1995, K.J. Aucoin, UCCB; Cape Breton County, Sydney, 15 July 1996, A.A. Risk, UCCB Hippodamia variegata (Goeze): This species first became established at Montreal, Quebec before 1984 (Gordon 1987). By 1992 it was widely distributed from northern New Jersey through New York, Connecticut and Vermont to Quebec (Wheeler 1993). The continuing range expansion to southern New Brunswick in 1993 and to Prince Edward Island and Colchester County, Nova Scotia in 1 995 was documented by Hoebeke and Wheeler ( 1 996). One year later, the spread has continued another few hundred kilometres to Antigonish County and Cape Breton County. Specimens: Antigonish County, Antigonish, 10 May 1996, B. Jones, STFXU; Cape Breton County, Sydney, September 1996, J. Meagher, UCCB The coccinellid fauna of Nova Scotia has changed dramatically in the past 15 years with the addition of four adventive species. The addition of these species is similar to what has occurred over much of northeastern North America (Gordon and Vandenberg 1991). It is interesting to compare the rate of range expansion for the four spe- cies, from their initial establishment in North America until they reached Cape Breton Island (Table 1). Cape Breton is at the northeastern limit of possible range expansion on continental North America. Further expansion requires a major water crossing to Newfoundland. I have collated the information based on the first established population in North America, as reported in Gordon 18 ENTOMOLOGICAL NEWS Table 1 : Average range expansion per year for four species of adventive coccinellids now estab- lished on Cape Breton Island, Nova Scotia. First established populations are from Gordon and Vandenberg (1991). The distance to Sydney, NS was measured from "The International Atlas" (McNally 1979). Species First established First Approximate Average range population Sydney, NS Distance expansion per record (km) year (km) Coccinella Bergen County, NJ 1985 1300 108 septempunctata Propylea Quebec City, PQ 1995 850 31 quatuordecimpunctata 1 968 Harmonia axyridis Abita Springs, LA 1995 3100 442 1988 Hippodamia Montreal, PQ 1996 1050 80 variegata <1984 and Vandenberg (1991) and the first record for Sydney, Cape Breton Island. Linear distance was based on a straight line measurement in "The Interna- tional Atlas" (McNally 1979). Rapid range expansion, with a minimum of 30 km/year and maximum of more than 400km/year, is clear for all four species. The rates of range expan- sion give a good picture of how quickly these species have colonized North America. It is possible these overestimate 'real' rates of range expansion. There have been many releases and attempts to introduce these species in many loca- tions in eastern North America (e.g. Chapin and Brou 1991; Wheeler 1993) and some releases or accidental introductions (e.g. Yanega 1996) closer to Nova Scotia than the first established population, may have been successful. How- ever, the rates are still useful because they emphasize the explosive potential for range expansion of introduced species in North America. Beyond impressive rates of range expansion, the newly established spe- cies will have an effect on the existing assemblage of coccinellids. Within five years of establishment on Cape Breton Island, Coccinella septempunctata be- came the most common species in anthropogenic habitats, as documented by the collections of UCCB students. Now, there are three more adventive spe- cies in Cape Breton. It remains to be seen whether one or more of these will displace C. septempunctata as the common coccinellid of disturbed habitats. There is also the potential for these adventive species to reduce populations of Vol. 1 09, No. 1 , January & February, 1 998 19 native species. Wheeler and Hoebeke (1995) have documented dramatic de- clines in the populations of the native C. novemnotata in eastern North America. Attention should be focused on the influence of these new adventives on the common native species such asAdalia bipunctata and C. trifasciata in the next few years. This note documents useful additions to our knowledge on range expan- sion and establishment of adventive coccinellids based on specimens in small regional insect collections. This type of collection should play a primary role in documenting population changes of these species in the next 15 years. ACKNOWLEDGMENTS I thank Eric Georgeson and Jeff Ogden of the Nova Scotia Department of Natural Resources Insect Collection, Belmont, Nova Scotia, Randy Lauffof St. Francis Xavier University, Antigonish, Nova Scotia, Barry Wright and Andrew Hebda of the Nova Scotia Museum of Natural History, Halifax, Nova Scotia, and Steve Marshall, University of Guelph, Guelph, Ontario for allowing me to examine specimens under their care and for discussions about coccinellids. Chris Thomson, Steve Marshall and Randy Lauff kindly reviewed the manuscript. I also thank the students of Biology 375 (Entomology) for providing many useful specimens. Financial support was pro- vided by Natural Sciences and Engineering Research Council and UCCB. LITERATURE CITED Chapin, J.B. and V.A. Brou. 1991. Harmonia axyridis (Pallas), the third species of the genus to be found in the United States (Coleoptera: Coccinellidae). Proc. Entomol. Soc. Wash. 93: 630-635. Coderre, D., E. Lucas, and I. Gagne. 1 995. The occurrence of Harmonia axyridis (Coleoptera: Coccinellidae) in Canada. Can. Entomol. 127: 609-61 1 . Day, W.H., D.R. Prokrym, D.R. Ellis, and RJ. Chianese. 1994. The known distribution of the predator Propylea quatuordecimpunctata (Coleoptera: Coccinellidae) in the United States, and thoughts on the origin of this species and five other exotic lady beetles in eastern North America. Entomol. News 105: 244-256. Gordon, R.D. 1985. The Coccinellidae (Coleoptera) of America North of Mexico. J. N. Y. Entomol. Soc. 93:1-912. Gordon, R.D. 1987. The first North American records of Hippodamia variegata (Goeze). J. N. Y. Entomol. Soc. 95: 307-309. Gordon, R.D. and N. Vandenberg. 1991. Field guide to recently introduced species of Coccinellidae (Coleoptera) in North America, with a revised key to North American genera of Coccinellini. Proc. Entomol. Soc. Wash. 93: 845-864. Hoebeke, E.R. and Wheeler, A.G. 1996. Adventive Lady Beetles (Coleoptera: Coccinellidae) in the Canadian Maritime Provinces, with new Eastern U.S. Records of Harmonia quadripunctata. Entomol. News 107: 281-290. Kidd, K.A., C.A. Nalepa, E.R. Day, and M.G. Waldvogel. 1995. Distribution of Harmonia axyridis (Pallas) (Coleoptera: Coccinellidae) in North Carolina and Virginia. Proc. Entomol. Soc. Wash. 97: 729-73 1 . Marshall, S.A. 1995. Ladybird, Fly Away Home. Seasons Spring 1995: 30-33. McNally, A. 1979. The International Atlas. Rand McNally Co., Chicago. 534pp. Schaefer, P.W., R.J. Dysart, and H.B. Specht. 1987. North American distribution ofCoccinella septempunctata (Coleoptera: Coccinellidae) and its mass appearance in coastal Delaware. Environ. Entomol. 16: 368-373. 20 ENTOMOLOGICAL NEWS Tedders, W.L. and P.W. Schaefer. 1994. Release and establishment of Harmonia axyridis (Co- leoptera: Coccinellidae) in the southeastern United States. Entomol. News 105: 228-243. Wheeler, A.G. Jr. 1993. Establishment of Hippodamia variegata and new records of Propylea quatuordecimpunctata in the eastern United States. Entomol. News 104: 102-1 10. Wheeler, A.G. Jr. and E.R. Hoebeke. 1995. Coccinella novemnotata in northeastern North America: historical occurrence and current status (Coleoptera: Coccinellidae). Proc. Entomol. Soc. Wash. 97:701-716. Yanega, D. 1996. Records of Propylea quatuordecimpunctata (Coleoptera: Coccinellidae) from Long Island, New York: evidence for a naturalized population before 1991. Entomol. News 107: 36-38. SCIENTIFIC NOTE RECENT INTERCEPTION OF LIVE KHAPRA BEETLE, TROGODERMA GRANARIUM (COLEOPTERA: DERMESTIDAE), AT THE PORT OF BALTIMORE, MARYLAND1 Michael L. Zimmerman, Julia A. Harron- The khapra beetle, Trogoderma granarium Everts, is a quarantined insect pest under United States Department of Agriculture (USDA) regulations. Each day, USDA Animal and Plant Health Inspection Service - Plant Protection Quarantine officers (USDA-APHIS-PPQ) examine a mul- titude of commodities entering the United States from all over the world looking for numerous foreign insect / plant / animal pests in order to ensure that none are present prior to entering the United States. Inspections are made of both commercial and personal freight arriving daily at ports of entry in vehicle, rail, airline, and ocean liner luggage/baggage. The khapra beetle is one of the major concerns for USDA-APHIS-PPQ officers when examining these imported goods. It is a serious pest of food items including stored cereal grains, dried fruits and other plant and animal materials, and does not occur in North America. Khapra beetles can survive long periods without food and make a habit of hiding in cracks and crevices in the warehouse making them hard to detect (Anonymous, 1983). In June 1996, a shipment of Basmati rice from Pakistan was off loaded at the Dundalk Marine Terminal, Port of Baltimore, Maryland, U.S.A. Investigators from the U.S. Food and Drug Administration (FDA) collected a surveillance sample to check for compliance under the adulterated food section of the Federal Food, Drug, and Cosmetic Act. The shipment consisted of 550 nylon weave bags, each containing eight - 1 1 pound cloth bags (sewn closed) of hulled, white grain rice. Examination of the rice at the FDA District Laboratory revealed the presence of live and dead maize weevil adults, Sitophilus zeamais Motschulsky (Curculionidae); sawtoothed grain beetle adults, larvae, and pupae, Oryzaephilus surinamensis (Linnaeus) (Cucujidae); red flour beetle adults and pupae, Tribolium castaneum (Herbst) (Tenebrionidae); and Trogoderma sp. dermestid adults and larvae. The Trogoderma larvae were initially identified as T. granarium Everts (khapra beetle) by the authors using Kingsolver's (1991) key to dermestid beetles. As a professional courtesy, FDA immediately contacted the local USDA-APHIS-PPQ entomologist at the Baltimore Port Office and the specimens were hand delivered for positive confirmation. (Continued on page 46) 1 Received June 10, 1997. Accepted July 1, 1997. 2 U.S. Food and Drug Administration, 900 Madison Avenue, Baltimore, Maryland 2 1 20 1 , U.S.A. Vol. 109, No. 1, January & February, 1998 21 CAMELOBAETIDIUS VARIABILIS (EPHEMEROPTERA: BAETIDAE), A NEW SPECIES FROM TEXAS, OKLAHOMA AND MEXICO1 N. A. Wiersema^ ABSTRACT: Camelobaetidius variabilis, new species, is described from larvae and from male and female adults. This species is common in the creeks and rivers of the hill country region of Texas and is also known from Oklahoma, and Nuevo Leon and Tamaulipas, Mexico. Characters are presented to separate the larvae of this species from C. similis and other related species. Lugo-Ortiz and McCafferty (1995) presented a taxonomic review of the North and Central American species of the genus Camelobaetidius Demoulin. As a result of their study, many previously accepted species were found to be variants of more widespread species and two new species were described, namely C. kondratieffi Lugo-Ortiz & McCafferty and C. similis Lugo-Ortiz & McCafferty. In 1996 specimens were collected by the author from the Guadalupe River in Texas that exhibited tarsal claw denticulation not typical of the only nomi- nal species, C. mexicanus (Traver and Edmunds), reported in Texas (Allen and Chao 1978, Henry 1986, McCafferty and Davis 1992, McCafferty and Provonsha 1993, Lugo-Ortiz and McCafferty 1995). Upon further examina- tion it was observed that these specimens had gills or osmobranchia on the pro- and mesothoracic segments. The presence of coxal gills on the middle coxae was previously unreported in this genus. Camelobaetidius similis de- scribed from Guerrero, Mexico, as well as other species known from Peru, do have thoracic gills on the prothorax. After examination of many specimens contained in my collection and the collection at the Brackenridge Field Labo- ratory of the University of Texas at Austin it became evident that this was an undescribed species. W. P. McCafferty and C. R. Lugo-Ortiz (Purdue Univer- sity, West Lafayette, Indiana) confirmed that this was a previously undescribed species and that the larvae differed from C. similis by size and tergal pattern- ing as well as other differences. The adults of C. similis are presently un- known. This new species is described here from the larvae and the adult male and female stage as Camelobaetidius variabilis, new species. All specimens were collected by the author and reside in the author's per- sonal collection (NAW) unless otherwise indicated. Institutions housing the 1 Received April 26, 1997. Accepted May 28, 1997. 2 4857 Briarbend Drive, Houston, TX 77035. ENT. NEWS 109(1): 21-26, January & February, 1998 22 ENTOMOLOGICAL NEWS other materials used in this study and acronyms are as follows: Brackenridge Field Laboratory, University of Texas at Austin (BFL), Purdue Entomological Research Collection (PERC), University of North Texas Entomology Museum (UNTEM), and the United States National Museum of Natural History (USNM). Life stage and material abbreviations are as follows: L-larvae, M-adult male, F-adult female E- larval exuvia slide mounted in Euparal. Camelobaetidius variabilis, NEW SPECIES (Figs. 1-4) Larva, (in alcohol) Body length: 5.6-7.0 mm; caudal filaments: 2.4-3.2 mm. Head: Colora- tion pale with area along coronal and frontal sutures pale olive-brown. Vertex pale olive-brown. Ocelli black. Turbinate portion of eyes in males reddish brown to orange-yellow, lateral lobes black. Antennae pale; sensilla not apparent. Labrum (Fig. 1 ) sclerotized posterolaterally, with one submedial and two to three submarginal simple setae on each side; intermediate setae absent. Right mandible 3+1+3 denticles; tuft of simple setae between prostheca and molar. Left mandible 3+1+3 denticles with short, broad process at base of molar. Maxillae robust; maxil- lary palps extending to galealacinia; palpal segment 2 broad about 0.5-0.75 length of segment 3. Labium robust; palpal segment one long; segment two shorter than one, with four to five simple setae dorsally and broad, rounded distal projection; segment three rounded apically, with numer- ous fine, simple setae; glossae with 10-11 simple setae medially, three to four dorsally and four to five laterally; paraglossae with five to six simple setae dorsally and two rows of simple setae distally. Thorax: Color pale to olive-brown with distinct pattern in live larvae (Fig. 4), faint in perserved specimens. Sterna pale to pale yellowish. Osmobranchia present on for- and midcoxae. Legs pale; femora rounded and often darkened distally with numerous fine, long, simple setae dorsally and pale olive-brown area medially. Tibiae armored ventrally with short, robust, simple setae; tarsi distinctly darken distally with row of short, simple setae ventrally with distal 2-3 about 1 .5-2 times the length of preceding setae. Tarsal claws with 5-9 denticles (rarely 9, see discussion) similar to Figs. 13, 15 & 16 in Allen and Chao (1978). Abdomen: Color pale olive- brown; Terga with olive-brown coloration faint on segments 4-5, 7 and posterior half of 10, with prominent submedian oblique markings and often a round pale area medially on each segment. Gills on segments 1 & 7 small and without tracheation; those on segments 2-6 usually with very little or no tracheation. Paraprocts with seven to eight spines. Caudal filaments pale, often darken distally with numerous, fine, simple setae. Median caudal filament subequal in length to cerci. Adult Male, (in alcohol) Body length excluding cerci: 5.6-6.2 mm; forewings: 5.6-6.0 mm; hindwing: 1 .0 mm; cerci: 8.8-9.2 mm. Body brownish tan, darkest at head, thorax, and abdomi- nal terga 7-10. Head: Turbinate portion of eyes orange-yellow, semi-elliptical, well separated and slightly divergent. Antennae pale. Thorax: Pronotum darker laterally. Legs pale. Wing veins and membrane hyaline. Hindwings as in Fig. 3. Abdomen: Terga distinctly darker than sterna. Terga brownish tan with segments 2-6 paler than segments 1 & 7-10. Usually darker laterally giving the appearance of a wide pale median line dorsally. Tracheation along pleura usually prominently darkened, but sometimes only faintly darkened. Sterna pale. Genitalia pale and as in Fig. 2. Cerci usually entirely pale, but sometimes with dark articulation in basal seg- ments. Adult Female, (in alcohol) Body length excluding cerci: 5.6-6.2 mm; forewings: 5.8-6.2 mm; hindwing: 1 .0 mm; cerci 7.0-7.5 mm. Body coloration as in male with terga distinctly darker than sterna. Abdominal segments 2-6 not distinctly paler than segments 1 & 7-10, due to presence of eggs. Hindwing as in Fig. 3. Legs and cerci light tan. Vol. 109, No. 1, January & February, 1998 23 Diagnosis. The larvae of C. variabilis are most similar to C. similis but can be separated from it by having a single thread-like gill on the midcoxae as well as forecoxae, labral setation lacking intermediate setae (as in Fig. 1), and tergal patterning (as in Fig. 4). In addition, C. variabilis also differs from C. similis by different mandibular denticulation and a greater number of marginal spines of the paraprocts, but these latter difference are of inconsistent diagnos- tic value. The larvae of C. variabilis are also very similar to C. mexicanus and Figs. 1 -3. Camelobaetidius variabilis, n. sp.: 1 . Labrum, dorsal view. 2. Male genitalia, ventral view. 3. Left hindwing. Fig. 4. Live male larva of Camelobaetidius variabilis, n. sp. from Devils River, Texas. Scale bar equals 1 mm. Photograph C.R. Nelson. 24 ENTOMOLOGICAL NEWS C. waltzi McCafferty, and in fact share similar labral shape and setation pat- terns. However, C. variabilis is easily separable by the presence of the coxal gills discussed above. In addition, C. variabilis is further separable from C. waltzi by having a rounded second segment of the labial palps, whereas C. waltzi has a medially pointed second segment of the labial palps. The larvae of C. waltzi also tend to have short, broad maxillary palps but this feature is of inconsistent diagnostic value. Camelobaetidius warreni is also similar to C. variabilis with respect to size and tarsal claw denticulation, but C. warreni lacks coxal gills and has labral setation with intermediate setae as in Fig. 6 Lugo-Ortiz and McCafferty (1995). Using the most recent key (Lugo-Ortiz and McCafferty 1995), the larvae of C. variabilis will key as C. similis. The following added couplet can be used to separate the larvae of these two species at that point in the Lugo-Ortiz and McCafferty key. Couplet to separate larvae C. variabilis from C. similis 1 . Coxal gills present on forecoxae only; labral setation as in Fig. 5 in Lugo-Ortiz and McCafferty (1995) similis 1 ' Coxal gills on fore and middle coxae; labral setation as in (Fig. 1 herein) variabilis According to Traver and Edmunds (1968) only Dactylobaetis sp. B de- scribed from Metlac (Vera Cruz?), Mexico and C. warreni (C. zenobia and C. cepheus were synonymized with C. warreni in Lugo-Ortiz and McCafferty 1995), are the only species known in the adult stage in North and Central America which possess a darker terga than sterna. Adult males of D. sp. B can be separated from C. variabilis by having semi-opaque abdominal segments 2-6 with tracheae not darkened and large turbinate eyes that are contiguous dorsally. I have found no reliable characters for the separation of adults of C. warreni from C. variabilis. Known distribution. USA: Oklahoma, Texas; MEXICO: Nuevo Leon, Tamaulipas. Material examined. HOLOTYPE: Male larva, USA: TEXAS: Williamson Co., Georgetown, San Gabriel River, riffles below San Gabriel Park, 20 Jan 1997 (USNM). ALLOTYPE: Female larva, same data and deposition as holotype. PARATYPES: One male and one female larvae same data as holotype (PERC). ADDITIONAL PARATYPES: Three male and five female larvae, USA: TEXAS: Comal Co., Guadalupe River about 11 mi., below Canyon Dam, 09 Nov 1996, same deposition as holotype. Other Material Examined. USA: TEXAS: Comal Co., Guadalupe River about 12.5 mi., below Canyon Dam, 16 Feb 1997 (8M, 3F, 4E); same location as before, but 21 Feb 1997 (7M, 2F). USA: TEXAS: COMAL CO., Guadalupe River about 1 Imi. below Canyon Dam, 17 Feb 1997 (L); same location as before, but 21 Mar 1997 (10 M, 3F). USA: TEXAS: Travis Co., Austin, Little Walnut Creek at Cameron Rd., 25 Mar 1997 (L); same location as before but 12 Apr 1997 (L). USA: TEXAS: WILLIAMSON CO., Tejas Camp, unnamed creek flowing into San Gabriel River, 19 Dec 1996 (L). USA: TEXAS: BASTROPCO., McKinney Roughs, Colo- rado River at Wilbarger Bend. 23 Nov 1996 (L). USA: TEXAS: VAL VERDE CO., Devils River, Dolan Falls Preserve. 27-29 Oct 1996 (L). USA: TEXAS: Edwards Co., Nueces River, Vol. 109, No. 1 , January & February, 1998 25 Barksdale, Hwy 55, 1 5 Mar 1993 C.R. Nelson, K.D. Alexander & S.M. Stringer (L; BFL). USA: TEXAS: Val Verde Co., Dolan Creek upstream from Devils River, 19 Mar 1993. C.R. Nelson, K.D. Alexander & S.M. Stringer (L; BFL). USA: TEXAS: Val Verde Co., Devils River at Dolan Falls Preserve, above falls, 1 3 Nov 1993 C.R. Nelson, K.D. Alexander & S.M. Stringer (L; BFL). USA: TEXAS: TRAVIS CO., Walnut Creek at 1-35, 06 June 1995 (L); same data but Bull Creek near Spicewoods Spring Road, 12 May 1995 (L). USA: OKLAHOMA: Murray Co., Davis, Honey Creek at Turner Falls Park, 14 Oct 1993, P.P. Wagner (2M, 3F, L; UNTEM). MEXICO: NUEVOLEON: Pobillo River at St. Hwy 115, near Linares, 15 May 1995, D.E. Baumgardner& B.C. Henry (L; UTEM). MEXICO: NUEVO LEON: Pilon River at unnamed road, across from General Teran, off St. Hwy 35, 15 May 1995, D.E. Baumgardner & B.C. Henry (L; UTEM). MEXICO: TAMAULIPAS: Branch of Chihue River at Hwy 101, ca. 12 mi S of Juamave, be- tween kilo marker 91 & 92; 17 May 1995, D.E. Baumgardner & B.C. Henry (L; UTEM). Etymology. The specific epithet is a Latin word meaning changeable. It is a reference to the apparent changeability in tarsal claw denticulation in early instars of this species and possibly the genus as a whole, see discussion. DISCUSSION Tarsal claw denticulation is variable in this species, but it most commonly exhibits 5-6 denticles, with 7-8 being less common. Of the approximately 300 larvae and exuvia examined by the author, one exuvia had 9 denticles on the tarsal claw of one of the metathoracic legs. Due to the variability in tarsal claw denticulation this character will not be useful in separating this species from the known species with 10 or less denticles on their tarsal claws unless specimens are collected in series. A life history study of C. variabilis has been conducted by Paul Wagner (Virginia Tech), and he has indicated to me that in early instars of this species there is a greater number of denticles per tarsal claw than is seen in the later instars and that a reduction in the number of denticles occurs within the first few instars. Similar observations in tarsal claw development have been made in species within the Family Heptageniidae, (see Traver and Edmunds 1968, pg. 638; Ide 1935). In Allen and Chao (1978) C. mexicanus was reported in Texas for the first time as Dactylobaetis mexicanus (see McCafferty and Waltz 1 990 for discus- sion on nomenclature change). The authors also presented a brief description of these specimens, in which they stated that the tarsal claw denticulation was of the warreni type 5-8 denticles. The original description of C. mexicanus found in Traver and Edmunds (1968) states that the tarsal claws of C. mexicanus have 5-6 denticles, this feature was also found to be consistent by Lugo-Ortiz and McCafferty (1995) in their review. This, along with the general tergal patterning described by Allen and Chao ( 1 978), suggests to me that their speci- mens were in actuality C. variabilis. This also calls into question many of the previous reports attributed to C. mexicanus in Texas. Camelobaetidius mexicanus was reported in Oklahoma for the first time by Reisen (1975) from Honey Creek in Murray County. I have examined 26 ENTOMOLOGICAL NEWS specimens from this location provided to me by P.P. Wagner (Virginia Tech) that are clearly Camelobaetidius variabilis. ACKNOWLEDGMENTS I would like to thank C. R. Nelson (University of Texas at Austin) for his suggestions and critique of the manuscript and for allowing me access to The Brackenridge Field Laboratory of the University of Texas at Austin (BFL). I would also like to thank P. F. Wagner (Virginia Tech) and D.E. Baumgardner (Aberdeen Proving Ground, Maryland) for loan of specimens. I would finally like to express my appreciation to W. P. McCafferty and C. R. Lugo-Ortiz (Purdue Uni- versity, West Lafayette, Indiana) for all their assistance and for their continual work towards Ephemeroptera systematics which made this description possible. LITERATURE CITED Allen, R. K. and S. M. Chao. 1978. Mayflies of the Southwest: new species and records of Dactylobaetis. Pan-Pac. Entomol. 54: 300-304. Henry, B.C., Jr. 1986. Mayflies of the Concho River, Texas. Southwest. Nat. 31: 15-21. Ide, P.P. 1935. Postembryonical development of Ephemeroptera (Mayflies). External charac- ters only. Canad. J. Research. 12: 433-78. Lugo-Ortiz, C.R. & W.P. McCafferty. 1995. The mayflies (Ephemeroptera) of Texas and their biogeograpic affinities. In:Current Directions in Research on Ephemeroptera (L.Corkum and J. Ciborowski, eds.), pp. 151-169. Canadian Scholars' Press Inc., Toronto, Canada. 478 pp. Lugo-Ortiz, C. R. and W. P. McCafferty. 1995. Taxonomy of the North and Central American species of Camelobaetidius (Ephemeroptera: Baetidae). Entomol. News 106: 178-192. McCafferty, W. P. & J. R. Davis. 1992. New and additional records of small minnow mayflies (Ephemeroptera: Baetidae) from Texas. Entomol. News 103: 199-209. McCafferty, W.P. & A.V. Provonsha. 1993. New species, subspecies, and stage descriptions of Texas Baetidae (Ephemeroptera). Proc. Entomol. Soc. Wash. 95: 59-69. McCafferty, W.P. and R.D. Waltz. 1990. Revisionary synopsis of the Baetidae (Ephemeroptera) of North and Middle America. Trans. Am. Entomol. Soc. 116: 769-799. Reisen, W.K. 1975. The ecology of Honey Creek, Oklahoma: spatial and temporal distributions of the macroinvertebrates. Proc. Okla. Acad. Sci. 55: 25-3 1 . Traver, J.R. and G.F. Edmunds, Jr. 1 968. A revision of Baetidae with spatulate-clawed nymphs (Ephemeroptera). Pac. Insects 10: 629-677. Vol. 1 09, No. 1 , January & February, 1 998 27 NEWLY REPORTED AND LITTLE KNOWN MAYFLIES (EPHEMEROPTERA) OF TEXAS1 N. A. Wiersema^ ABSTRACT: Ten species are newly reported for Texas along with new distributions for little known mayfly species in Texas. Baetis armillatus, B. bimaculatus, B. dubius, Labiobaelis frondalis, Procloeon rufostrigatum, P. viridoculare, Ephemerella dorothea, Leptophlebia cupida, L. intermedia and Ephoron album are reported in Texas for the first time. Lugo-Ortiz & McCafferty (1995) presented the results of a biogeographic study of mayfly fauna and a checklist of 76 nominal species reported for Texas. Recent revisionary work (Henry 1993), a status change (Lugo-Ortiz & McCafferty 1994), new reports (Baumgardner et al. 1997) and a new species description (Wiersema 1 998) raised this number to 94 nominal species reported for Texas. Recent collections in the Big Thicket and Hill Country areas of Texas and examination of the author's personal collection revealed several species of mayflies previously unreported for Texas with new distributions for little known species. Collection records reported here raise the number of nominal species reported for Texas to 104 and cast doubt on the validity of a previous report of Ephemerella inermis Eaton. All specimens were collected by the author and reside in the author's per- sonal collection unless otherwise indicated. Institutions housing the other ma- terials used in this study and acronyms are as follows: Purdue Entomological Research Collection (PERC) and the Burian Ephemeroptera Collection (BEC). New state records are marked with an asterisk (*). Life stage and material abbreviations are as follows: L-larvae, M-adult male, F-adult female, rM-reared adult male, rF-reared adult female. BAETIDAE Apobaetis indeprensus Day New Collection Records. WILLIAMSON CO., Georgetown, San Gabriel River at San Gabriel Park, 07Oct 1995(2M, IF). Remarks. This species was first reported in Texas by McCafferty & Davis (1992). There was some hesitation by those authors in reporting their larval specimens as A. indeprensus because the larval stage of A. etowah (Traver) is unknown. The adult specimens I collected clearly are A. indeprensus. This species is now known to occur in the Balconian and Texan biotic provinces in Texas (Lugo-Ortiz and McCafferty 1995). 1 Received June 2, 1997, Accepted July 14, 1997. 2 4857 Briarbend Drive, Houston, TX 77035. ENT. NEWS 1 09( 1 ): 27-32, January & February, 1 998 28 ENTOMOLOGICAL NEWS *Baetis armillatus McCafferty & Waltz Collection Records. MONTGOMERY CO., New Caney, Caney Creek at US 59, 04 Jan 1997 (L); 08 Mar 1997 (L); 10 Mar 1997 (L). MONTGOMERY CO., New Caney, Peach Creek at FM 1485, 16 June 1997(L,7M, IF, IrM, IrF). AUSTIN CO., San Bernard River at I- 10, near Sealy, 20 May 1997(L, IrM). Remarks. This species appears to be widely distributed across the eastern half of the country, and is also known from Ontario, Alberta, (Berner & Pescador 1988) and has recently been re- ported in Colorado in the West (McCafferty et al. 1993). *Baetis bimaculatus (Bemer) Collection Records. MONTGOMERY CO., New Caney, Caney Creek at US 59, 08 Mar 1997 (L); 10 Mar 1997 (L). MONTGOMERY CO., New Caney, Peach Creek at FM 1485, 16 June 1997(L, 2rM, 4rF). Remarks. This species was described in Bemer (1946) from adults and larvae collected in Florida and is currently known also from Alabama, Georgia, Mississippi, and South Carolina (Bemer & Pescador 1988). The larval specimens collected on June 16 1997 agree well with Berner's description of this species with one exception, the first as well as the seventh pair of gills are suffused with reddish brown pigmentation. The collection of this species in Texas extends its known range westward. *Baetis dubius (Walsh) Collection Records. COMALCO., Guadalupe River about 1 1-12 miles below Canyon Dam, 16 Feb 1997 (L, 1M); same data but about 7-8 miles below Canyon Dam, 16 Sept 1996 (1M). COMAL CO., Sattler, Guadalupe River about 4-5 miles below Canyon Dam, 08 Feb 1997 (L). HAYS CO., Blanco River at Post Road, near Kyle, 14 Apr 1997 (1M); 16 Apr 1997 (3M); 19Apr 1997(L, IrM, IrF), L(PERC); 20 Apr 1997 (L, IF); 24 Apr 1997 (L, 1M), 1 M (PERC); 05 May 1997 (L, IrM, IrF). WILLIAMSON CO., Georgetown, San Gabriel River at San Gabriel Park, 28 Apr 1997 (2F); 19 May 1997 (L, IrM, IrF). Remarks. This species is widely dispersed throughout the eastern half of North America (Burks 1953; McCafferty & Waltz 1990) and the collection of this species in Texas extends its known range westward. Labiobaetis dardanus (McDunnough) New Collection Records. HAYS CO., Blanco River at Post Rd., near Kyle, 1 4 Apr 1 997 (L); 1 6 Apr 1997 (L); 19 Apr 1997 (L); 24 Apr 1997 (L, IrM, 2rF). TRAVIS CO., Austin, Colorado River at Hwy 1 83, 22 Sept 1 996 ( 1 F). Remarks. This species has been reported in the Austroriparian biotic province by Baumgardner et al. (1997). The data reported here place this species in the Balconian province as well. * Labiobaetis frondalis (Traver) Collection Records. NEWTON CO., Nichols Creek Hwy 87, 30 Dec 1996 (L). MONTGOM- ERY CO., New Caney, Peach Creek at FM 1485, 05 Jan 1997 (L); 16 June 1997 (L, 3rM, 4rF). MONTGOMERY CO., New Caney, Caney Creek at US 59, 08 Mar 1997 (L); 10 Mar 1997 (L), 26 May 1997(L, IrM, IrF). Remarks. This species is widely distributed across the eastern half of North America (Morihara & McCafferty 1979). Labiobaetis propinquus (Walsh) New Collection Records. SAN JACINTO CO., Winters Bayou at Hwy 150, 10 mi. SW of Cold Springs, 23 Dec 1996 (L). MONTGOMERY CO., New Caney, Caney Creek at US 59, 26 May 1997(L, 3rM, 2rF). Vol. 1 09, No. 1 , January & February, 1 998 29 Remarks. This species was first reported in Texas by McCafferty & Davis (1992) from the San Bernard River which borders Colorado and Austin County, near the eastern limits of the Texan biotic province. Lugo-Ortiz & McCafferty (1995) listed this primarily eastern species as exist- ing in the Texan and Austroriparian provinces, but gave no distribution in the Austroriparian province. The data reported here place this species in the Austroriparian province. Paracloeodes minutus (Daggy) New Collection Records. TRAVIS CO., Austin, Colorado River at Hwy 183 bridge, 22 Sept 1996 (6F). TRAVIS CO., Austin, Bull Creek near Spicewood Springs Rd., 12 Oct 1996 (2F). MONTGOMERY CO., New Caney, Peach Creek at FM 1485, 16 June 1997 (L, IrM, 3M, IF). Remarks. Paracloeodes minutus was first reported in Texas by McCafferty & Davis ( 1 992) and is currently known to occur in the Austroriparian, Kansan, and Texan biotic provinces (Lugo- Ortiz & McCafferty 1995). The Travis County data listed here extends its known range in Texas into the Balconian biotic province. *Procloeon rufostrigatum (McDunnough) Collection Records. MONTGOMERY CO., New Caney, Caney Creek at US 59, 26 May 1997 (IrF). Remarks. Procloeon rufostrigatum is known from Manitoba, New Brunswick, Ontario and Quebec in Canada and Illinois, Kentucky, Maryland, Michigan, Minnesota, Pennsylvania, and Wisconsin in the United States (Lowen & Flannagan 1992). The discovery of P. rufostrigatum in southeast Texas significantly extends its known range southward. *Procloeon viridoculare (Berner) Collection Records. HAYS CO., Blanco River at Post Rd., near Kyle, 1 4 Apr 1 997 (L); 1 9 Apr 1997 (18M); 20 Apr 1997 (49M, IF); 24 Apr 1997 (52M), 2M (PERC); 05 May 1997 (larva). AUSTIN CO., San Bernard River at 1-10, near Sealy, 20 May 1997 (L, IrM). MONTGOMERY CO., New Caney, Caney Creek at US 59, 26 May 1997 (L). MONTGOMERY CO., New Caney, Peach Creek at FM 1485, 16 June 1997 (L, 3F, 2rM, IrF), IrM, IF and L (PERC). Remarks. This species is currently known from Alabama, Florida, Georgia, Mississippi and South Carolina (Berner & Pescador 1988, Unzicker and Carlson 1982: p. 3.85) and was recently reported in Michigan and Pennsylvania (Waltz & Munro 1996). The discovery of this species in the Balconian, Texan and Austroriparian provinces in Texas extends its known range westward. BAETISCIDAE Baetisca obesa (Say) New Collection Records. MONTGOMERY CO., New Caney, Caney Creek at US 59, 27 Dec 1 996 (L); 04 Jan 1997(L). Remarks. This species was first reported in Texas by Lewis (1986) as occurring in the Neches River drainage system in the Austroriparian province. The data reported here place Baetisca obesa in the San Jacinto River drainage system within the Austroriparian province as well. EPHEMERELLIDAE *Ephemerella dorothea Needham Collection Records. MONTGOMERY CO., New Caney, Peach Creek FM 1485, 05 Jan 1997 (L); 08 Mar 1997 (L, 2rM, 2rF); 10 Jan 1997 (L, 4rM, 3rF), 2rM, IrF and L (PERC). Remarks. The adult specimens I reared vary somewhat from published descriptions of this species (McDunnough 1931, Traver 1935, Burks 1953, Allen & Edmunds 1965) in that male genitalia are reminiscent of E. inermis Eaton, a western mountainous species. Larvae of the eastern E. dorothea and western E. inermis are also very similar morphologically, however the Texas larvae are clearly of the dorothea type, possessing coarse vermiform cuticular stippling typical of E. dorothea but not E. inermis. Adult variation may be a result of genetic drift in a 30 ENTOMOLOGICAL NEWS fringe population and there is little doubt the two species are closely related, if not sister species (pers. comm. W. P. McCafferty). Baumgardner et al. (1997) reported E. inermis from Texas based on one larva apparently collected in the Balconian province in which the date and collector where unknown. The farthest east E. inermis is known to occur are the headwaters of the Pecos and Rio Grande Rivers in New Mexico and its presence in Texas does not fit any biogeographic patterns (pers. comm. W.P. McCafferty). In light of this information and the presence of E. dorothea in East Texas, it is assumed that the E. inermis report is the result of a misidentification of E. dorothea. Eurylophella doris (Traver) New Collection Records. SAN JACINTO CO., Winters Bayou at Hwy 150, 23 Dec 1996 (L). MONTGOMERY CO., New Caney, Caney Creek at US 59, 27 Dec 1996 (L). NEWTON CO., Trout Creek at Hwy 87, 30 Dec 1996.(L). MONTGOMERY CO., New Caney, Peach Creek at FM 1 485, 05 Jan 1 997 (L). NEWTON CO., Cow Creek at Hwy 87, 1 4 Mar 1 995, D. Hulsen & C. Mcduffie (L). Remarks. This species has been reported in Texas by Baumgardner et al. ( 1 997). Using the key of Funk & Sweeny ( 1 994), specimens from Texas have morphological characteristics of E. doris as opposed to the closely related E. temporalis (broader fore femora, and longer posteral-lateral projections of the 2nd and 3rd abdominal segments). HEPTAGENIIDAE Stenonema exiguum Traver New Collection Records. AUSTIN CO., San Bernard River at I- 10, near Sealy, 03 Feb 1997 (L). MONTGOMERY CO., New Caney, Caney Creek at US 59, 27 Dec 1996 (L); 04 Jan 97 (L); 05 Jan 1997 (L, 14rM, 12rF). MONTGOMERY CO., New Caney, Peach Creek FM 1485, 05 Jan 1997 (L); 10 Mar 1997 (L, 1M). Remarks. This species has been known from the Austroriparian biotic province (Lugo-Ortiz & McCafferty 1995, Bednarik & McCafferty 1979) and the collection record listed here from Aus- tin County extends its known distribution into the Texan biotic province. Stenonema terminatum (Walsh) New Collection Records. BELL CO., Lampasas River at FM 1 1 23 1 4 Dec 1 996 (L); Same data as previous but at Dice Grove Rd., 14 Dec 1996(L). COMAL CO., Guadalupe River about 11 mi. below Canyon Dam, 7 Dec 1996 (L). HAYS CO., Blanco River at Post Rd., near Kyle, 24 Apr 1997 (IF). MONTGOMERY CO., New Caney, Caney Creek at US 59, 27 Dec 1996 (L); 04 Jan 1997 (L); 05 Jan 1997 (L, lOrM, 8rF). MONTGOMERY CO., New Caney, Peach Creek at FM 1485, 05 Jan 1997 (L); 10 Mar 1997 (4M, IrF). Remarks. This species has been known from the Austroriparian and Balconian biotic provinces (Lugo-Ortiz & McCafferty 1995). The collection of this highly variable and widespread species (Bednarik & McCafferty 1979) in the Texan province (Bell County data) was expected. ISONYCHIIDAE Isonychia arida (Say) New Collection Records. MONTGOMERY CO., New Caney, Caney Creek at US 59, 04 Jan 1997 (L); 08 Mar 1997 (L); 10 Mar 1997 (L). MONTGOMERY CO., New Caney, Peach Creek at FM 1485, 05 Jan 1997 (L); 08 Mar 1997 (L, 2rM, 3rF); 10 Mar 1997 (L, 3rM, 2rF). Remarks. This species is currently being reported in Texas by Baumgardner et al. (1997). The data reported here represent a new county record. LEPTOPHLEBIIDAE *Leptophlebia cupida (Say) Collection Records. NEWTON CO., Trout Creek at Hwy 87, 30 Dec 1996 (3rM, IrF), IrM and IrF(BEC). Vol. 109, No. 1 , January & February, 1998 31 Remarks. Leptophlebia cupida was found cohabiting with L. intermedia (Traver) in Trout Creek. This genus is being revised by Dr. Steve Burian, Southern Connecticut State University, who provided confirmations. Besides L. intermedia (see below), the only other species of this genus known for Texas is the distinctive L. bradleyi (Needham). ^Leptophlebia intermedia (Traver) Collection Records. NEWTON CO., Trout Creek at Hwy 87, 30 Dec 1996 (8rM, 4rF), 2rM and IrF(BEC). Remarks. This species is widely distributed across the eastern half of North America. Paraleptophlebia volitans (McDunnough) New Collection Records. MONTGOMERY CO., New Caney, Caney Creek at US 59, 04 Jan 1997 (L); 08 Mar 1997 (L, 2rM, IrF); 10 Mar 1997 (L). MONTGOMERY CO., New Caney, Peach Creek at FM 1485, 10 Mar 1997 (L). Remarks. This species has been known from Quebec (McDunnough 1924) to Florida and west into Louisiana, and it was recently reported in Texas by Baumgardner et al. (1997). The data reported here represent a new county record. POLYMITARCYIDAE *Ephoron album (Say) Collection Records. MONTGOMERY CO., New Caney, Peach Creek at FM 1485, 16 June 1997(2F). Remarks. Ephoron album is a common western and midwestem species (McCafferty 1 994) and Texas represents the southern limit of its known range. ACKNOWLEDGMENTS I would like to express my appreciation to the following individuals: W.P. McCafferty (Purdue University) for confirmation and discussion of the Ephemerella dorothea specimens as well as further assistance. S.K. Burian (Southern Connecticut State University) for confirmation of the Leptophlebia specimens. C.R. Nelson (University of Texas at Austin) for loan of specimens and for all his assistance. D. Hulsen for the loan of specimens. B.C. Kondratieff (Colorado State University) for some helpful pre-manuscript advice. D.E. Baumgardner and J.H. Kennedy (Uni- versity of North Texas) for providing me a copy of their manuscript. LITERATURE CITED Allen, R.K. & G.F. Edmunds, Jr. 1965. A revision of the genus Ephemerella (Ephemeroptera: Ephemerellidae) VIII. The subgenus Ephemerella in North America. Ento. Soc. Am. Misc. Publ. 4: 243-282. Baumgardner, D.E., J.H. Kennedy, & B.C. Henry. 1997. New and additional records of Texas mayflies (Insecta: Ephemeroptera). Trans. Am. Entomol. Soc. (in press) Bednarik, A.F. & W.P. McCafferty. 1979. Biosystematic revision of the genus Slenonema (Ephemeroptera: Heptageniidae). Can. Bull, of Fish. & Aq. Sci. 201: 1-72. Berner, L. 1946. New species of Florida mayflies (Ephemeroptera). Florida Entomol. 28: 60- 82. Berner, L. & M.L. Pescador. 1988. The mayflies of Florida, revised edition. Univ. Press, Gainesville, Florida. 415 pp. Blair, W.F. 1950. The biotic provinces of Texas. Texas J. Sci. 2: 93-1 17 Burks, B.D. 1953. The mayflies, or Ephemeroptera, of Illinois. Bull. 111. Nat. Hist. Surv. 26: 1- 216 32 ENTOMOLOGICAL NEWS Funk, D.H. & B.W. Sweeny. 1994. The larvae of eastern North American Eurylophella Tiensuu (Ephemeroptera: Ephemerellidae). Trans. Amer. Entomol. Soc. 120: 209-286. Henry, B.C., Jr. 1993. A revision of Neochoroterpes (Ephemeroptera: Leptophlebiidae) new status. Trans. Amer. Entomol. Soc. 119:317-333. Lowen & Flannagan. 1992. Nymphs and imagoes of four North American species ofProcloeon Bengtsson with description of anew species (Ephemeroptera: Baetidae). Can. Entomol. 124: 97-108. Lugo-Ortiz, C.R. & W.P. McCafferty. 1994. New records of Ephemeroptera from Mexico. Entomol. News 105:17-26. Lugo-Ortiz, C.R. & W.P. McCafferty. 1995. The mayflies (Ephemeroptera) of Texas and their biogeographic affinities. In: Current Directions in Research on Ephemeroptera (L. Corkum and J. Ciborowski, eds.), pp. 151-169. Canadian Scholars' Press Inc., Toronto, Canada. 478 pp. Lugo-Ortiz, C.R. & W.P. McCafferty. 1996. New Central American and Mexican records of Ephemeroptera species. Entomol. News 107: 303-310. Lewis, S.P. 1986. Records of Baetisca obesa (Say) (Ephemeroptera: Baetiscidae) in Southeast Texas and Southwest Louisiana. Southwest. Nat. 31: 396-398. McCafferty, W.P. 1994. Distributional and classificatory supplement to the burrowing mayflies (Ephemeroptera: Ephemeroidea) of the United States. Entomol. News 105: 1-13. McCafferty, W.P. & J.R. Davis. 1992. New and additional records of small minnow mayflies (Ephemeroptera: Baetidae) from Texas. Entomol. News 103: 199-209. McCafferty, W.P., R.S. Durfee, and B.C. Kondratieff. 1993. Colorado mayflies (Ephe- meroptera): An annotated inventory. 1993. Southwest. Nat. 38:252-274. McCafferty, W.P. & R.D. Waltz. 1990. Revisionary synopsis of the Baetidae (Ephemeroptera) of North and Middle America. Trans. Am. Entomol. Soc. 116: 769-799. McDunnough, J. 1924. New Canadian Ephemeridae with notes, II. Canad. Entomol. 56: 90-98, 113-122, 128-133. Morihara, D.K. & W.P. McCafferty. 1979. The Baetis larvae of North America (Ephemeroptera: Baetidae). Trans. Am. Entomol. Soc. 105: 139-221. Traver, J.R. 1935. Part II. Systematics, pp. 239-739. In: Needham, J.G., J.R. Traver, and Y.C. Hsu. eds., The Biology of Mayflies. Comstock Publ., Ithaca, New York. Unzicker, J.D. & P. Carlson. 1982. Ephemeroptera, pp. 3.1-3.97. In A.R. Brigham, W.U. Brigham, and A. Gnika, eds. Aquatic insects and Oligocheates of North and South Carolina. Midwest Aquatic Enterprises, Mahomet, Illionois. Waltz, R.D. & J.B. Munro. 1996. Procloeon viridocularis (Ephemeroptera: Baetidae) from Michigan and Pennsylvania, new range extensions with comments on the species. Entomol. News. 107: 166-168. Wiersema, N.A. 1998. Camelobaetidius variabilis, new species (Ephemeroptera: Baetidae) from Texas and Oklahoma. Entomol. News. 109: 21-26. Vol. 109, No. 1 , January & February, 1 998 33 REDESCRIPTION OF NERTHRA PRAEC1PUA (HETEROPTERA: GELASTOCORIDAE) FROM CHILE1 J. T. Polhemus2, T. Cekalovic K.3 ABSTRACT: Nerthra praecipua, known previously from the holotype female, has been redis- covered in Chile. The species is redescribed, supplementing the incomplete original description of the damaged female holotype. This species belongs to the Nerthra alalicollis group, which occurs in Australia and Chile. Nerthra praecipua Todd was described by Todd (1957) from a unique fe- male in the Reed Collection (acquired by C. J. Drake, now housed in the Na- tional Museum of Natural History, Washington, D. C.) that had been badly damaged by dermestids, rendering a complete description impossible. A single additional female has been collected by the junior author on Chiloe Island, Chile, providing the first definitive locality and permitting further description of the species. It is held in the J. T. Polhemus Collection (JTPC). Nerthra praecipua Todd Nerthra praecipua Todd, 1957:151, Fig. 9 (Holotype, female, Chile, Reed Collection, now in USNM); Todd, 1961:472 (checklist). Supplemental description to that of Todd (1957). Female: Length 8.10 mm; width of pronotum 5.50 mm; width of abdomen 6.02 mm. General color stramineous, with scattered dark markings on veins of hemelytra, head, me- dian lobe of pronotum, scutellum; depressions on head, median lobe of pronotum, fuscous to piceous; anterior 2/3 of lateral margins of abdominal tergites III-VI dark; scutellum with lateral tumescence fuscous, anterolateral angles piceous. Thoracic venter mostly stramineous, mesos- ternum medially mostly piceous except median anterior margin and medial protuberance; ab- dominal venter mostly dark, with scattered yellowish markings, laterally and distally lighter. Legs stramineous, with fuscous markings; fore femora broadly infuscated, with fascia at distal 21 3; middle and hind femora with fascia at basal 1/3, irregular annulus at distal 2/3, distal extreme, fuscous; tibia dark distally. Structural characters: Head excavate anteriorly, without apical tubercle but with small api- cal protuberance, with superapical and lateral tubercles (Fig. 4), set with clavate setae, ocelli absent. Pronotum with median lobe raised, sculptured, set with scattered short clavate setae. Abdominal tergites sinuate, extending laterally beyond hemelytra (see Todd, 1957, fig. 9), with groups of moderate-length clavate setae on posterolateral angles. Hemelytra without membrane, partially fused, with thin medial line of demarcation between hemelytra, set with short clavate setae along lateral margins; network of veins more extensive than depicted by Todd (loc. cit.). 1 Received March 15, 1997. Accepted May 15, 1997. 2 Colorado Entomological Museum, 31 15 S. York St., Englewood, CO. 801 10. 3 Casilla 764, Concepcion, CHILE. ENT. NEWS 109(1): 33-36, January & February, 1998 34 ENTOMOLOGICAL NEWS Fore femur with edge of anterior dilation forming about a 50° angle with posterior edge; anterior trochanter with two black-tipped denticles (Fig. 5). Abdominal stemites almost symmetrical (Fig. 2); ovipositor lobes slightly asymmetrical, located posterodorsally (Figs. 1 , 3). Figures 1 - 5. Nerthra praecipua Todd, female, (scale bar - 1.0 mm). 1 - 3. Abdominal terniinalia. 1. dorsal view. 2. ventral view. 3. lateral view. 4. Head, dorsal view. 5. Anterior trochanter, with black-tipped denticles anteriorly. Material examined. 1 female, Chile, Chiloe Island, Puente la Caldera, 41 ° 40' S, 74° 02' W, 15 Feb. 1996, T. Cekalovic K. (JTPC). Comparative notes. TV. praecipua does not resemble any other South American species, and is the only American member of the N. alaticollis group. It is the only American species with sternite VII prolonged posteriorly and lacking emargination, with the female genital structures located dorsally. In Todd's (1960) key to Australian species of Nerthra, N. praecipua drops at couplet 3 because it lacks ocelli; thus, in this regard, it is unique in the alaticollis group. Todd (loc. cit.) stated that species of the alaticollis group have an api- cal tubercle, but in N. adspersa (Stal) and TV. stall (Montandon) it is no more than a small apical protuberance, similar to that of N. praecipua. Habitat. The habitat in which the single specimen was collected is sur- rounded on all sides by forest, situated in hills of moderate altitude. The veg- etation is native and semihumid. The trunks and limbs of trees in various states of decay are covered with mosses, lichens, and adhering ferns of the genus Hymenophyllum. The vegetative community is a typical damp Nothofagus forest. The specimen was separated from a sample of humus (TC-427) by means Vol. 1 09, No. 1 , January & February, 1 998 35 of a Berlese funnel, in company with various arachnids and insects including Peloridiidae (Coleorrhyncha: Peloridora sp.). Zoogeography. N. praecipua provides another link between the hemi- pteran faunas of Austral South America and Australia. Concerning N. praecipua, Todd (1957) stated that "The projecting median portion of the last abdominal sternite and general appearance would seem to indicate that this species is most closely related to the species of the alaticollis group found in Australia." This is an interesting observation (supported by independent investigations by JTP) because Chile and adjacent austral Argentina are Gondwanan in origin, and several diverse groups found there are phylogenetically linked to New Zealand, Australia, and New Guinea (e.g. mayflies, Edmunds, 1 975; Aradidae, Isoderminae, Monteith, 1 982:649; Peloridiidae, Evans, 1 98 1 ; Nothofagus, Ash, 1982:355). The projecting abdominal sternite VII of females and dorsal location of the female genital structures is a synapomorphy for the clade comprising the Nerthra alaticollis, laticollis, elongata, and rugosa groups established by Todd (1955, 1960). The laticollis group occurs in Australia and Melanesia (mainly Solomon Islands and New Guinea), the elongata and alaticollis groups occur on Australia, but the latter also on Chile. The rugosa group has only a few species that occur on various islands and continental land masses, and appar- ently all are halophilous and are rarely if ever found far from the seashore. The associated Peloridiidae, a hemipteroid family of almost exclusively flightless bugs (only one species with rare macropters), is of interest because of its association with, and zoogeographical parallels to, flightless Nerthra species. The primary ecological difference between them is that peloridiids are primarily cool-adapted moss inhabitants not yet found on New Guinea, whereas the flightless Nerthra are mostly warm-adapted litter inhabitants that are absent from New Zealand. Evans (1981:395) speculated that peloridiids will be found in the Nothofagus forests of New Guinea. ACKNOWLEDGMENTS We thank D. A. Polhemus and R. T. Schuh for their constructive reviews. Support for this research was provided in part by grant BSR-9020442 from the National Science Foundation, Washington, D. C. This research was carried out as an adjunct faculty member of Colorado State University. We thank these organizations for their continued support of research into the system- atics and zoogeography of aquatic Heteroptera. LITERATURE CITED Ash, J. 1982. The Nothofagus Blume (Fagaceae) of New Guinea, pp. 355-380 In: Gressitt, J. L. ed., Biogeography and Ecology of New Guinea, Junk, The Hague. Edmunds, G. F., Jr. 1975. Phylogenetic biogeography of mayflies. Ann. Missouri Bot. Gard. 62:251-263. 36 ENTOMOLOGICAL NEWS Evans, J. W. 1981. A review of the present knowledge of the family Peloridiidae and new genera and new species from New Zealand and New Caledonia (Hemiptera: Insecta). Rec. Austr. Mus. 34:381-406. Monteith, G. B. 1982. Biogeography of the New Guinea Aradidae (Heteroptera), pp. 645-657 In: Gressitt, J. L. ed., Biogeography and Ecology of New Guinea, Junk, The Hague. Todd, E. L. 1955. A taxonomic revision of the family Gelastocoridae (Hemiptera). Univ. Kans. Sci. Bull. 37:277-475. Todd, E. L. 1957. Five new species of Gelastocoridae with comments on other species (Hemi- ptera). Proc. Entomol. Soc. Wash. 59:145-162. Todd, E. L. 1960. The Gelastocoridae of Australia (Hemiptera). Pac. Insects 2:171-194. Todd, E. L. 1961. A checklist of the Gelastocoridae (Hemiptera). Proc. Haw. Entomol. Soc. 17:461-476. SCIENTIFIC NOTE AN UNUSUAL OCCURRENCE OF CADDISFLIES (TRICHOPTERA: PHRYGANEIDAE) IN A PENNSYLVANIA POPULATION OF THE PURPLE PITCHER PLANT, SARRACENIA PURPUREA R. Hamilton IV2, R. L. Petersen2, R. M. Duffield2'3 While studying the contents of one hundred and four leaves collected from a population of purple pitcher plants in ChristnerBog, Somerset County, Pennsylvania, on March 13, 17, and 22, 1996, five caddisfly larvae were recovered. Three larvae were alive in their cases, one larva was dead in a case, and one was dead and covered by a fungus identified as a species of Saprolegnia (Saprolegniaceae) (Coker, 1923). The larvae were identified as belonging to the genus Oligostomis (Phryganeidae). Adult caddisflies collected in the same site in May 1996, were identified as Oligostomis ocelligea (Walker). This is the first report of Oligostomis larvae in pitcher plants. The presence of this caddisfly is probably related to the flooding of the habitat during the late winter and early spring. Purple pitcher plants are carnivorous plants, usually found in nitrogen-deficient, Sphagnum- dominated bogs or swamps. A wide variety of species of insects and other arthropods are asso- ciated with the pitcher plant. These invertebrates can be classified as regular inhabitants, prey, occasional associates, or plant herbivores (Rymal and Folkerts, 1982). Caddisfly larvae have been reported in pitcher plants (Brower and Brower, 1971; Cresswell, 1991), yet it is not clear what their presence signified. The water level in wetlands can rise significantly after snow melt or rain, submerging the pitcher plant leaves. This would permit aquatic organisms to move inside and/or on top of the leaves. Pittman et al. (1996) proposed that aquatic predators may gain entrance to the pitchers during periods of high water and flooding, and feed on the inhabitants. While it is possible caddis larvae may have inadvertently entered the pitcher plant leaves due to flooding, we suggest that the larvae recovered in this study entered the pitcher plant leaves in search of food. Oligostomis (Continued on page 50) 1 Received April 28, 1997. Accepted July 28, 1997. 2 Department of Biology,Howard University, Washington, D.C. 20059. 3 To whom correspondence should be sent. Vol. 109, No. 1, January & February, 1998 37 A NEW SPECIES OF SYMPHITONEURIA (TRICHOPTERA: LEPTOCERIDAE) FROM SABAH, MALAYSIA ' Trond Andersen^, Jolanda Huisman^ ABSTRACT: Symphitoneuria sabaensis n. sp. from Sabah (Borneo), East Malaysia is described and figured as male and female imago. The species is the second Symphitoneuria to be described from outside the Australian biogeographical region. A key to the adult males of Symphitoneuria is provided. The genus Symphitoneuria was described by Ulmer ( 1 906) for Notanatolica exigua (McLachlan). Later, Mosely (1936) described the genus Loticana for Notanatolica opposita (Walker), at that time only known from the female. Banks (1939) suggested that Loticana was a synonym of Symphitoneuria, an opinion formally confirmed by Mosely and Kimmins (1953). Symphitoneuria is char- acterized by the male forewing venation in which the media and cubitus are fused with the lower margin of the discoidal cell. In the female the wing vena- tion is normal, and the hind wings are similar in both sexes. The male genitalia, however, closely resemble those found in the genus Triplectides Kolenati. In his study on the phylogeny and classification of the longhorned caddisfly family Leptoceridae, Morse (1981) included Symphitoneuria in the tribe Triplectidini of the subfamily Triplectidinae. The phylogeny of Triplectidinae was outlined by Morse and Holzenthal (1987), but they did not resolve the phylogeny of Triplectides sensu lato, and were inclined to regard the various monophyletic lineages identified as subgenera only. By suggesting the alterna- tive, that Symphitoneuria, Lectrides Mosely in Mosely and Kimmins, Symphitoneurina Schmid, and Triplectidina Mosely, presently considered sepa- rate genera, apparently evolved from within Triplectides, the latter genus would be rendered paraphyletic. As implied by Morse (1989) further studies on the taxonomy, morphology and zoogeography of the Triplectides complex are es- sential to resolve this problem. Including the new species described here, the genus Symphitoneuria now comprises seven species. With the exception of S. dammermanni Ulmer, all previously described species appear to be restricted to the Australian biogeo- graphic region (Morse, 1989). Symphitoneuria dammermanni was described by Ulmer ( 1 95 1 ) from the Sumba Islands, Indonesia, just west of Weber's line. Ulmer (1906) recorded females of S. opposita from Sulawesi (Celebes). Later, 1 Received June 19, 1997. Accepted July 28, 1997. 2 University of Bergen, Museum of Zoology, Museplass 3, N-5007 Bergen, Norway. 3 University of Minnesota, Department of Entomology, 219 Hodson Hall, 1980 Folwell Avenue, St.Paul, Minnesota 55108-6125 USA. ENT. NEWS 109(1): 37-46, January & February, 1998 38 ENTOMOLOGICAL NEWS Martynov ( 1 93 1 ) reported a male of this species (as Notanatolica opposita), also from Sulawesi, but did not describe it, even though the male of 5. opposita was not known at that time. Banks (1913) further recorded 5. opposita (as Notanatolica opposita) from the Philippines. Neboiss (1977) listed 5. opposita from Sulawesi, but Morse (1989) suggested that these records might be misidentifications of S. dammermanni or the specimens might belong to some yet undescribed species. With the distribution of 5. opposita in doubt, the de- scription of S. sabaensis, n. sp., from Sabah (Borneo) confidently extends the known range of the genus further into the Oriental biogeographic region. The material treated here was collected by the junior author during several trips to Sabah, Sarawak and Brunei between 1986 and 1990. Habitats collected ranged from lowland to montane (50 to 3300 m a.s.l.) primary forest types. MATERIAL AND METHODS The material was collected predominately with light traps and the speci- mens were preserved in 70% alcohol. Methods used in preparing, examining, and illustrating genitalia are those commonly used in the study of Trichoptera. The terminology is adopted from Morse and Neboiss (1982). Measurements are reported as total lengths, antennae length is given as the longest measured. Measurements are given as ranges, followed by the mean when more than three measurements were taken, and (in parentheses) by the number of specimens measured. The holotype and paratypes of Symphitoneuria sabaensis n. sp. are depos- ited in the Nationaal Natuurhistorisch Museum (formerly Rijksmuseum van Natuurlijke Historic), Leiden, The Netherlands (RMNH); paratypes are also deposited in the University of Minnesota Insect Collection, St. Paul, Minne- sota, USA (UMSP), the National Museum of Natural History, Smithsonian In- stitution, Washington, USA(USNM), and in the Museum of Zoology, University of Bergen, Norway (ZMBN). Symphitoneuria sabaensis, Andersen and Huisman, NEW SPECIES Figs. 1-16 Adult male (n=10, if not otherwise stated). Forewing length 8.8-10.9, 10.0 mm; hindwing length 6.0-7.8, 7.2 mm. Eye 0.43-0.56, 0.5 1 mm wide. Antennae at least 35.2 mm long including 0.45-0.52, 0.48 mm long antennal scape. Maxillary palp segment lengths (in mm): 0.42-0.52, 0.46; 0.61-0.72, 0.67; 0.71-0.84, 0.76; 0.37-0.47, 0.42; 0.72-0.85, 0.81. Color (in alcohol) yel- lowish brown. Wings (Figs. 1 -2). Forewing with crossvein sc-r broadened, and stigma present; apical forks I, II, and V present, i.e. forks of Sj+2, $3+4, and Cui+2; f°r^ I reaching basally beyond S; discoi- dal cell short and broad, slightly shorter than its stem, and nearly as broad as fork II; thyridial cell vestigial, veins M and Cu seemingly forming single, thick, composite longitudinal vein with 83+4 for apical five-sevenths of discoidal cell, with five veins, 83, 84 + MA, MP, Cuj, and Cu2, arising Vol. 109, No. 1, January & February, 1998 39 SC-R Cu1 Cu2 S1 Figures 1-4. Symphitoneuria sabaensis n. sp. wings. 1. Male forewing. 2. Male hindwing. 3. Female forewing. 4. Female hindwing. Abbreviations: Cu - cubitus, dc - discoidal cell, MA - anterior media, MP = posterior media, R - radius, S - sector, SC - subcosta, tc - thyridial cell; I, II, V - primary apical cells, or "forks", I, II, and V. 40 ENTOMOLOGICAL NEWS from it; Cu2 recurved about 100° basally, and arising from composite vein slightly basally to where 83+4 joins the composite vein; nygma faint, rectangular, present in fork II. Hindwing shorter and broader than fore wing; apical forks II and V present; fork V originating about one- half of distance between origin of discoidal cell (fork of S) and fork of M; nygma faint, rounded, present in fork II. Genitalia (Figs. 5-9). Segment IX narrow, subrectangular. Superior appendage about three- fifths as long as tergum X, narrow, setose, in dorsal view broader with rounded apex; with small lobe mesally at base bearing one strong seta. Tergum X about as long as inferior appendage; in lateral view with distinct step-like elevation middorsally and narrow apical portion projecting caudad; in dorsal view rounded, deeply cleft apically, with broadly rounded projections laterally, single to partly double row of 6-9, 8 (5) setae dorsally on each side; and 6-10, 9 (5) sensilla apically; with transverse reinforcing band ventrally at base of cleft and lateral, diagonal reinforc- ing ridge internally towards base. Inferior appendage with broad base, dorsally slightly convex; in ventral view with posteromesal corner rounded, forming nearly straight angle (90°); subbasally bearing few, long setae ventrolaterally; apicodorsal lobe rounded apically, setose, slightly shorter than harpago, with accessory projection mesally bearing 4-5, 5 (5) strong, spine-like setae, long- est 0.064-0.076, 0.069 (5) mm long; in ventral view harpago slender, slightly sinuous, with apex hooked mesad; basoventral lobe curved caudad, setose, about one-third as long as inferior ap- pendage. Phallus subcylindrical with slightly sinuous sides; ejaculatory duct narrow, conspicu- ous; phallotremal sclerite about half as long as tubular portion of phallus. Adult female (n-10, if not otherwise stated). Forewing length 8.9-9.9, 9.4 mm; hindwing length 6.5-7.7, 7.2 mm. Eye 0.45-0.50, 0.47 mm wide. Antennae at least 27.9 mm long including 0.40-0.48, 0.45 mm long antennal scape. Maxillary palp segment lengths (in mm): 0.43-0.52, 0.47; 0.60-0.72, 0.68; 0.71-0.84, 0.79; 0.40-0.45, 0.42; 0.76-0.92, 0.85. Color (in alcohol) as in male. Wings (Figs. 3-4). Forewing with crossvein sc-r broadened and stigma present; apical forks I, II, and V present; fork I sessile; discoidal cell long, subequal to thyridial cell in length; nygma faint, rectangular, present in fork II. Hindwing shorter and broader than forewing; apical forks II and V present; fork V long, originating about one-third of distance between origin of discoidal cell (fork of S) and fork of M; nygma faint, rounded, present in fork II. Genitalia (Figs. 10-14). Abdominal segment VIII with sternum narrow, triangular, setose; in ventral view with posterior margin broadly rounded. Segment IX with tergum broad; pleuron with distinct, rounded corner laterally. Dorsal setose lobe narrow, triangular, attached along ven- tral surface; in dorsal view broadly rounded to subtriangular; with short, triangular semimembra- nous sensilla-bearing process, bearing one or occasionally two setae. Lamella with longitudinal striae on ventral surface; with double to triple row of slightly curved setae along posterolateral margin; apparently flexible both in posterior and in ventral direction, when in dorsal, (open) position narrowly triangular in lateral view (Fig. 10), in dorsal view subrectangular and pointing posterolaterad (Fig. 1 3); when turned more ventrad, (closed position), subtriangular with broadly rounded apex in lateral view (Fig. 11), in dorsal view rounded, pointing posteriad (Fig. 14); in some specimens apex turned further ventrad, (fully closed position), overlapping, forming roof- shaped hood. Gonopod plate apparently somewhat flexible, with straight or convex ventral mar- gin in lateral view; in ventral view with posterior margin subrectangular, with small, rounded, median excavation. Spermathecal sclerite oval, broadly rounded anteriorly; in lateral view slightly curved, subrectangular; in all spent females twisted or positioned askew. Egg mass (Figs. 15-16). Consisting of strings of eggs apparently glued together with trans- parent secretion, with scattered long setae, probably originating from the wing fringe, encrusted in the surface. Outline oval or slightly ovoid, with one side rounded, other side flatter with dis- tinct groove where next layer of eggs begin. Egg mass 2.19-2.62 (2) mm long, 1.87-1.89 (2) mm wide, and 1.34-1.38 (2) mm thick. Larva and pupa. Unknown. Vol. 109, No. 1, January & February, 1998 41 sup app -ap do Iffl ph sc -- ej du 8 •sup app Figures 5-9. Symphitoneuria sabaensis n. sp. male genitalia. 5. Lateral. 6. Ventral. 7. Dorsal. 8. Phallus, ventral. 9. Phallus, lateral. Abbreviations: ac pr - accessory projection of apicodorsal lobe of inferior appendage, ap do - apicodorsal lobe of inferior appendage, bv - basoventral lobe of inferior appendage, ej du - ejaculatory duct, har- harpago, inf app- inferior appendage, IX - segment IX, ph sc - phallotremal sclerite, sup app - superior appendage, X - tergum X. 42 ENTOMOLOGICAL NEWS , do se _-- s-b p 11 — - viii 10 lam lam --__ do se IX- 13 go pi sp sc 12 14 Figures 10-14. Symphitoneuria sabaensis n. sp. female genitalia. 10. Lateral, with lamella in open position. 1 1 . Lateral, with lamella in closed position. 12. Ventral. 13. Dorsal, with lamella in open position. 14. Dorsal, with lamella in closed position. Abbreviations: do se =• dorsal setose lobe, go pi - gonopod plate, IX - segment IX, lam - lamella, s-b p - sensilla-bearing process, sp sc - spermathecal sclerite, VIII - sternum VIII. Vol. 109, No. 1, January & February, 1998 43 16 Figures 15-16. Symphitoneuria sabaensis n. sp. egg mass. 15. Ventral (flat side). 16. Lateral (blunt end). Type material. HOLOTYPE: Cf, Malaysia, Sabah, Long Pa Sia area E of kampong, 25. xi- 8.xii.l987, 1000 m a.s.l.. Malaise trap 4, C. van Achterberg (RMNH). PARATYPES: 1 Cf , Long Pa Sia, riverbank near airstrip, 04°25'N 115°43'E, 1000 m a.s.l., 21 .x. 1986, J. Huisman (UMSP); 5 9 , Long Pa Sia, airstrip, 04°24'N 115°43'E, 1000m a.s.l., 1 6.iv. 1987, at light, J. Huisman &J. van Tol (RMNH 3 9 , NMNH 9 , ZMBN 9 ); 1 9 , Long Pa Sia, 04"25'N 1 15°43'E. 1000 m a.s.l., 1 . xii. 1 987, J. Huisman & C. van Achterberg (UMSP); 4 9 , Long Pa Sia, airstrip, 04°24'N 115°43'E, 1000m a.s.l., 7. xii. 1987, at light, J. Huisman (RMNH); 1 Cf, Sapulut, kampong, road- side, 4.V.1987, 04°42'N 1 16°29'E, 290 m a.s.l., at light, J. Huisman (ZMBN); 5 Cf , 4 9 , 7 km S Nabawan, near old airstrip, pond, 1 4.xi. 1 987, 05°02'N 1 1 6°27'E, 400 m a.s.l., at light, J. Huisman 6 R. de Jong (RMNH); 1 Cf, 2 9, 1 1 km S Nabawan, pond on roadside, 15.xi.1987, 04"57'N 116°27'E, 400m a.s.l., at light, J. Huisman &R.de Jong (UMSP); 1 Cf, 1 9, 12.5 km S Nabawan, on road to Kg. Pamuntariah, 1st bridge, 16.xi.1987, 04°57'N 116°27'E, 400 m a.s.l., at light, J. Huisman & R. de Jong (RMNH Cf , ZMBN 9 ); 3 Cf , Kundassang kampong, Sg. Liwagu, on bridge, 23.viii.l 986, 06°00'N 1 16°34'E, at light, J. Huisman (UMSP 2 Cf, ZMBN Cf); 1 Cf , 1 9, Kundassang kampong, Sg. Liwagu, on bridge, 23.xi.1986, 06°00'N 116°34'E, 1185 m a.s.l., J. Huisman (NMNH Cf , UMSP 9 ). Etymology: named after Sabah, the northeastern part of the Island of Borneo, using the Latin suffix -ensis, denoting place, locality or country of origin. KEY TO MALES OF SYMPHITONEURIA 1 Apicodorsal lobe of inferior appendage with setose, accessory projection mesally 2 1 ' Apicodorsal lobe of inferior appendage lacking setose, accessory projection mesally 6 2 Tergum X about as long as inferior appendage; fork I in forewing sessile 3 2' Tergum X distinctly longer than inferior appendage, in dorsal view triangular, pointed, with deeply cleft apex; fork I in forewing with short stem (Mosely and Kimmins, 1953: 260, figs. 180-181) 5. exigua (McLachlan) 3 Forewing with discoidal cell short and broad, with crossvein r-s reaching discoidal cell in distal one-fifth; fork V in hindwing long, originating distinctly closer to base than fork of M 4 44 ENTOMOLOGICAL NEWS 3' Forewing with discoidal cell long and narrow, with crossvein r-s reaching discoidal cell about two-thirds from base; fork V in hindwing short, originating about the same distance from base as fork of M (Mosely and Kimmins, 1953: 263, fig. 183) 5. opposita (Walker) 4 Tergum X in dorsal view with apex rounded to bluntly triangular 5 4' Tergum X in dorsal view with apex subtruncate (Morse, 1989: 207, fig. 2) 5. dammermanni Ulmer 5 Tergum X in dorsal view gradually widened basally, with apex bluntly triangular; accessory mesal projection of apicodorsal lobe of inferior appendage with more than 6 spine-like setae (Neboiss, 1987: 140, fig. 40) S. ampla Korboot 5' Tergum X in dorsal view with broadly rounded and projecting sides and rounded apex; accessory mesal projection of apicodorsal lobe of inferior appendage with 4-5 strong spine-like setae (Figs. 5-7) S. sabaensis n. sp. 6 Inferior appendage with harpago fused almost entirely to apicodorsal lobe, basoventral lobe not forked (Mosely and Kimmins, 1953: 268, fig. 187) 5. wheeleri Banks 6' Inferior appendage with harpago free, basoventral lobe forked (Neboiss, 1986: 222, figs. 38-40) 5. licmetica Neboiss DISCUSSION The male wing venation of 5. sabaensis most closely resembles that of S. ampla Korboot and 5. dammermanni, especially in the comparatively short and broad forewing discoidal cell and the long hindwing fork V. However, it differs from both in having Cu2 arising from composite vein slightly basally to where 83+4 joins the vein. The setose mesal accessory projection on the apicodorsal lobe of the inferior appendage of the male genitalia, groups 5. sabaensis with 5. ampla, S. dammermanni, S. exigua, and S. opposita. It can be distinguished, however, from all known species of Symphitoneuria by the rounded apex and broadly rounded and projecting sides of tergum X. The female of S. sabaensis can be separated from other described females except 5. exigua in having the posterior margin of the gonopod plate subrec- tangular, with a small, rounded, median excavation. However, S. exigua ap- pears to have the posterior margin of sternite VIII with a shallow, broadly V-shaped, median excision, while the posterior margin in S. sabaensis is evenly rounded. Biology. Little seems to be known about the ecology and habitat requirements of most of the Symphitoneuria species. St Clair (1994) described the larvae of S. exigua and stated that it is usually found in small, sluggish, often turbid lowland streams and swamps. According to lilies (1969) 5. ampla appeared to be the most abundant Trichoptera species in Lake Pinde and Lake Aunde at about 3,600 m a.s.l. on Papua New Guinea. The present specimens were taken in localities ranging from 290 to 1 1 85 m a.s.l., mostly along fast flowing rivers, but also close to stagnant blackwater pools. Vol. 109, No. 1 , January & February, 1998 45 In some genera of Triplectidini the females carry egg masses at the tip of their abdomen (see e.g. St Clair, 1993). Korboot (1963) studied the life history of S. exigua, figuring and describing the egg mass as spherical and covered in a layer of mucilage. The egg mass was dropped by the female from a height of 3 to 4 inches into the water. Although none of the females of S. sabaensis n. sp. had their egg masses still attached when studied, the females apparently carry the egg mass between sternum VIII and the gonopod plate. Sternum VIII ap- pears to be movable and a cavity is formed in the pleural region of segment VIII. Setae probably originating from the wing fringe and imbedded in the egg mass were seen inside the cavity in several of the females. The apparent flex- ibility of the lamella, which allow for movement in both posterior and ventral directions, might also be an adaptation for manipulation of the egg mass. ACKNOWLEDGMENTS The senior author wants to express his gratitude to Ralph Holzenthal and the University of Minnesota for making it possible for him to spend his sabbatical at the Minnesota Insect Collec- tion, and to the University of Bergen for financial support. The junior author received financial support for the field work in Sabah from Uyttenboogaart- Eliasen Foundation, MelchiorTreub Foundation, and the National Museum of Natural History in Leiden. In Sabah, the staff of Kinabalu National Park was very supportive particularly Anthea Lamb-Phillips and Fui-Lian Inger-Tan; Jan van Tol, Cees van Achterberg and Rienk de Jong participated in the fieldwork. We both want to thank Ralph Holzenthal and John C. Morse for all support and useful com- ments on the manuscript. LITERATURE CITED Banks, N. 191 3. On a collection of neuropteroid insects from the Philippine Islands. Proc. Entomol. Soc. Wash. 15: 170-180. Banks, N. 1939. New genera and species of neuropteroid insects. Bull. Mus. Comp. Zool. Harv. Univ. 85: 439-504. lilies, J. 1969. Trichoptera from the high mountain Lakes Pinde and Aunde, New Guinea. Pac. Insects 11: 487-493. Korboot, K. 1963. Biological studies of some Caddis Flies (Trichoptera) from south east Queensland. Univ. Queensland Pap., Ent., Brisbane 1: 241-274. Martynov, A.B. 1931. Resultats scientifiques du voyage aux Indes orientates neerlandaises de LL. AA. RR. le prince et la princesse Leopold de Belgique. Trichoptera. Mem. Mus. Hist. Nat. Belg., Brussels (h.s.) 4 (2): 13-15. Morse, J.C. 1981. A phylogeny and classification of family-group taxa of Leptoceridae (Trichoptera). Pp. 257-264. In: G.P. Moretti, [ed.], Proceedings of the Third International Symposium on Trichoptera. W. Junk Publ., The Hague, Ser. Entomologica 20: 472 pp. Morse, J.C. 1989. A redescription of Symphitoneuria dammermanni ; Ulmer, 1951 (Trichoptera: Leptoceridae). Entomol. Mitt. Zool. Mus. Hambg 9: 205-212. Morse, J.C. and Holzenthal, R.W. 1987. Higher classification of Triplectidinae (Trichoptera: Leptoceridae). Pp. 139-144. In: M. Bournaud and H. Tachet [eds.] Proceedings of the Fifth International Symposium on Trichoptera. W. Junk Publ., The Hague, Ser. Entomologica 39: 397 pp. 46 ENTOMOLOGICAL NEWS Morse, J.C. and Neboiss, A. 1982. Triplectides of Australia (Insecta: Trichoptera: Leptoceridae). Mem. Nat. Mus. Vic. 43: 61-98. Mosely, M.E. 1 936. A revision of the Triplectidinae, a subfamily of the Leptoceridae (Trichoptera). Trans R. Entomol. Soc. London 85: 91-130. Mosely, M.E. and Kimmins, D.E. 1953. The Trichoptera (caddisflies) of Australia and New Zealand. Br. Mus. (Nat. Hist.), Lond. 550 pp. Neboiss, A. 1977. A taxonomic and zoogeographic study of Tasmanian caddis-flies (Insecta: Trichoptera). Mem. Nat. Mus. Vic. 38: 1-208. Neboiss, A. 1986. Taxonomic changes in caddis-fly species from the south' west Pacific- Austra- lian Region with descriptions of new species (Insecta: Trichoptera). Mem. Mus. Vic. 47: 213- 223. Neboiss, A. 1987. Identity of species of Trichoptera described by K. Korboot 1964-65 (Insecta). Mem. Mus. Vic. 48: 131-140. St Clair, R.M. 1993. Life histories of six species of Leptoceridae (Insecta: Trichoptera) in Victoria. Aust. J. Mar. Freshwater Res. 44: 363-379. St Clair, R.M. 1994. Some larval Leptoceridae (Trichoptera) from South-eastern Australia. Rec. Australian Mus. 46: 171-226. Ulmer, G. 1906. Neuer Beitrag zur Kenntnis Auser-Europaeischer Trichopteren. Notes Leyden Mus. 28: 1-128. Ulmer, G. 1951. Kocherfliegen (Trichoptera) von den Sunda-Inseln. Teil I. Arch. Hydrobiol., Suppl. 19: 1-528. (continued from page 20) The Basmati rice was immediately detained by FDA after live insects were found and the presence of khapra beetle suspected. Once the larvae found in the rice were positively confirmed as khapra beetle by USDA, the rice shipment was fumigated with methyl bromide under USDA supervision. A reconditioning plan was proposed by the importer to salvage the rice, but it was subsequently refused. After no further response by the consignee, the shipment was issued a notice of refusal and was ordered to be exported or destroyed under U.S. Custom's supervision. A personal communication with J. F. Cavey, Entomologist, USDA-APHIS-PPQ in Riverdale, MD, provided additional records of khapra beetle interceptions into the United States. He stated that within the last five years, 35 interceptions of khapra beetle by USDA-APHIS-PPQ officers were recorded at ports of entry into the United States, with the majority of these interceptions occurring in Texas, Georgia, and California. Ours is the only record of the khapra beetle found entering Baltimore, MD during this time. ACKNOWLEDGMENTS We thank Susan Broda-Hydorn, Identification Specialist, USDA-APHIS-PPQ, Baltimore, MD for her confirmation of our identifications and Joseph F. Cavey, Entomologist, USDA-APHIS- PPQ, Riverdale, MD, for his contribution of unpublished data on khapra beetle interception records by the USDA into the U.S. since October, 1992. LITERATURE CITED Anonymous. May 1983. Khapra Beetle. APHIS Facts, Animal and Plant Health Inspection Service, US. Dep. Agric., Wash., DC. Kingsolver, J. M. 1991. Dermestid beetles (Dermestidae, Coleoptera) [pp. 115-135]. In: Insect and Mite Pests in Food: An Illustrated Key, Volume 1 . J. R. Gorham (ed.), USDA Handbook No. 655, US. Gov't. Print. Off., Wash., DC. vii + 767 pp. Vol. 109, No. 1, January & February, 1998 47 A NEW SPECIES OF TOMOCERUS (S.S) (COLLEMBOLA: TOMOCERINAE) FROM CHINA1 Y-T. Ma2- K. A. Christiansen3 ABSTRACT: A new Chinese species, Tomocerus cheni, from China is described. It is similar to the Japanese T. cuspidatus, Borner 1 909, but differs in body color, unguiculus and other features. Nearly 50 species have been described in the genus Tomocerus (s.l.)\ how- ever, only 12 species were described or reported from China: Tomocerus (Tomocerina) minutus Tullberg 1 876 from Shanxi and 1 1 species in the genus Tomocerus (s.s.): 4 from Tibet (monticolus, obsculus, parvus and zayensis Huang and Yin 1981), 2 from Yunnan (varius Folsom 1899 andfolsomi Denis 1929), caputiviolaceus Lee 1975 and cuspidatus Borner 1909 from Taiwan, kinoshitai Yosii 1954 from Hunan, ocreatus Denis 1948 from Zhejiang, and sibiricus Reuter 1891 from Hebei. A new species of the subgenus Tomocerus (s.s.) cheni, from Anhui Province, is described here. Tomocerus (S.S.) cheni, NEW SPECIES Color: Background color pale yellow with purplish blue pigment. Head dark with irregular pale spots; eye patches dark. Antennal segments I, IV and basal part of III pigmented, II and most distal part of III pale. Thoracic segments and anterior margin of abdominal segment I irregularly pigmented. Abd. V & VI sometimes with a few scattered pigment patches. Base of coxae with dark pigment. Tibiotarsus with scattered pigment (Fig. 1 ). Head: Antennae short, respectively 0.5-0.8 and 2.7-3.1 times as long as body and Cephalic di- agonal; ratios of Ant. I-IV - 1.0/1.4-2.0/4.5-7.3/1.2-2. Eyes 6+6, A & B largest, E & F smallest (Fig. 2). Labral setae 4/5,5,4, marginally with 4 recurving spinules. Head capsule anteriorly with 2,4 large setae, posteriorly with 43-54 small setae (Fig. 3). Body: Thoracic macrochaetae and bothriotricha as shown in Fig. 4. Trochanteral organ not clearly seen. Unguis rather slender; a pair of well developed pseudonychia 1/3-1/2 as long as inner edge of unguis; inner teeth 5-7, 5-7 & 5-6 respectively on legs I-III. Unguiculus lanceolate with 1 outer tooth and 1 -2 inner teeth. Tenent hair well developed, as long as inner edge of unguis, apex spatulate (Fig. 5). Tibiotarsus with numerous pointed smooth setae in different sizes; ventral side with 3-4(5), 6(4,8), 6-8 large blunt spinelike setae respectively on legs I-III (Fig. 6). Abdominal macrochaetae and bothriotricha on segments I-V as shown in Fig. 4. Tenaculum unsealed with 4+4 teeth, corpus with 8-12 smooth setae (Fig. 7). Ventral tube scaled, posterior face with numerous smooth setae in different sizes, anterior face not clearly seen, lateral flap with about 70 smooth setae in different sizes (Fig. 8). Ratios of manubrium/dens/mucro - 2.S-4/ 5-5.6/1. Manubrium laterally with a row of large ciliate setae on each side, these setae more * strongly tapered near tip; dorsally with 2 longitudinal bands (setaceous stripes, Yosii 1967) of numerous weakly ciliate to striate, acuminate setae in different sizes, about 20 of them very 1 Department of Biology, Nanjing University, Nanjing 210093 P.R. China 2 Grinnell College Grinnell Iowa 50112 U.S.A. ENT. NEWS 109(1 ): 47-50, January & February, 1998 48 ENTOMOLOGICAL NEWS Tomocerus cheni, All figures of type specimens. Fig. 1. Habitus; 2. Eyes of left side; 3. Cephalic chaetotaxy; 4. Chaetotaxy of body; 5. Hind foot complex; 6. Large setae of hind tibiotarsus; 7. Tenaculum; 8. Lateral flap of ventral tube; 9. Left half of distal part of manubrium (dorsal view); 10. Dental spines; 1 1. Mucro; 12. Setulae at base of macrochaeta and bothriothrix. Vol. 109, No. 1, January & February, 1998 49 large; no scales present between setaceous bands (Fig. 9). Dental spines as 4<3)-5/3(2)-5,I,2( 1 ,4), I; heavy chestnut brown and each with 2(3) secondary teeth (spinules) near base. 1-2 (rarely 3) small, finely ciliate, spiny setae present interior to basal dental spines (Fig. 10). Mucro elongate with numerous ciliate setae; outer dorsal lamella with 5-7 intermittent teeth; outer basal tooth with a corner toothlet; apical and anteapical teeth subequal (Fig. 1 1 ). Upper anal flap of Abd. VI with 7 large, striate primary setae arranged in an irregular transverse row. Scales brownish, hyaline and heavily striated. Each trunk macrochaeta surrounded by 3-6 setulae, bothriotricha without setulae at base (Fig. 12). Size: Maximum length 3.6 mm. Type materials Holotype: 9. China: Anhui Province, Yellow Mt., VII- 16- 1990, leaf litter in deciduous forest and in moss, collection number 8223. Paratypes: 799. same data as holotype, collection numbers 8223 & 8213 Other locality: 1 9- Anhui: Jinzhai County: Tiantangzhai Park, collection number 8306. All specimens will be deposited in the Department of Biology, Nanjing University. Etymology: This species is named after Prof. Jian-xiu Chen in the Department of Biology, Nanjing University, whose help was essential. DIAGNOSIS The large manubrial dorsal setae ("principal setae" of Yosii 1967) are acumi- nate in T. cheni sp. nov. rather than blunt. This species is very similar to T. cuspidatus Borner 1909; however, it differs from the latter as shown below: cheni cuspidatus* cuspidatus** Maximum body length Scales on tenaculum Blunt "principal" setae on manubrium 3.6 absent absent 6.0 present 2+2,1 6.5 Spinules on dental spine 2(3) 3-6 3-5 * sensu Yosii 1967 ** sensu Lee 1975 ? unknown ACKNOWLEDGMENTS We would like to express our thanks to Mr. Liu Ren-hua who inked all the final drawings for the present paper. Thanks are also given to Prof. Byung-Hoon Lee and Prof. Jian-xiu Chen for their great help to this work. This work was published with a grant from Grinnell College. 50 ENTOMOLOGICAL NEWS LITERATURE CITED Denis, J. R. 1929. Notes sur les Collemboles recoltes dans ses voyages par le Prof. F. Silvestri. I. Seconde note sur les Collemboles d'Extreme-Orient. Bull. Lab. Zool. Portici 22: 305-320. Denis, J. R. 1948. Collemboles d'Indochine recoltes de M. C. Dawydoff. Notes d'Entomologie Chinoise, Musee Heude, Vol XII, Fasc. 17: 183-259. Huang, Fu-Sheng, 1981. Insects of Xizang, Vol. 1, pp. 41-46, China Science Press, Huang, Fu-Sheng, 1 995. Three new recorded species of Tomocerus Nicolet in China (Collembola: Tomoceridae). Sinozoologia 12: 192-193. Lee, B. H., 1975. Etude de la Faune coreenne des Insectes Collemboles. VI. Sur la Famille des Tomoceridae, edaphiques, avec la Description de quatre nouvelles especes et d'une nouvelle sous-espece. Bull. Mus. Nat. Histoire Nat. 3e sen, no 317, juillet-aout, Zool. 224: 946-961. Stach, J., 1964. Materials to the Knowledge of Chinese Collembolan Fauna. Acta Zoologica CracoviensialX(l): 1-26. Yosii. R., 1967. Studies on the Collembolan Family Tomoceridae, with Special Reference to Japanese Forms. Contributions from Biol. Lab. Kyoto Univ. No. 20: 1-54. (continued from page 36) is primarily a predator, then secondarily an herbivore and detritivore (Wiggins, 1984). The internal environment of the pitcher plant leaves offers live larvae of other species, plant materials, and numerous decomposing remains. Analysis of the larval gut contents revealed numerous pieces of insect cuticle that may have been consumed while the larvae were inside the pitchers. ACKNOWLEDGEMENTS We would like to thank the U.S.D. A.-Forest Service (Eastern Region), especially Keith Jensen and Martin MacKenzie for partial support during the course of this project. We also thank Dr. Oliver S. Flint, Jr. (Department of Entomology, Smithsonian Institution, Washington, D.C.) for helpful suggestions. R. Hamilton is supported in part by a fellowship from a National Science Foundation Graduate Traineeship in Plant Biology (GER 93549 1 6). Voucher specimens are held in the Department of Biology, Howard University, Washington, D.C. (RMD). Ecological report #5. LITERATURE CITED Brower, J. H. and Brower, A. E. 1 97 1 . Notes on the biology and distribution of moths associated with the pitcher plant in Maine. Entomol. Soc. Ont. Proc. 101:79-83. Coker, N. C. 1923. The Saprolegniaceae with notes on other water molds. Univ. N. Carolina Press. Chapel Hill, N.C., 201 pp. Cresswell, J. E. 1 99 1 . Capture rates and composition of insect prey of the pitcher plant Sarrace- nia purpurea. Am. Midi. Nat. 125:1-9. Pitt man, J. L., T. S. Turner, L. Frederick, R. L. Petersen, M. E. Poston, M. Mackenzie, and R. M. Duffield. 1 996. Occurrence of alderfly larvae (Megaloptera) in a West Virginia population of the American pitcher plant, Sarracenia purpurea L. (Sarraceniaceae). Ent. News 107:137- 140. Rymal, D. E. and G. W. Folkerts. 1982. Insects associated with pitcher plants (Sarracenia: Sarraceniaceae), and their relationship to pitcher plant conservation: a review. J.Alabama Acad. Sci. 53:131-151. Wiggins, G. B. 1984. Trichoptera, In: An Introduction to the Aquatic Insects of North America (2nded.) Merrill, R. W. and Cummins, K. W. (eds). Kendall/Hunt, Dubuque, Iowa, pp. 27 1 -3 1 1 . Vol. 109, No. 1, January & February, 1998 51 TOMOCERUS (s.s.) SPINULUS (COLLEMBOLA: ENTOMOBRYIDAE), A NEW SPECIES OF CHINESE SPRINGTAIL1 J-X. Chen2, K. A. Christiansen3 ABSTRACT: A new Chinese species, Tomocerus spinulus, from Anhui Province China is de- scribed here. It is closest to the Pakistan species T. asoka Yosii, 1965 and the Japanese species T. viridis Yosii, 1967. Nearly 50 species have been described in the genus Tomocerus s.l.; how- ever, only 12 species were described or reported from China: Tomocerus (Tomocerina) minutus Tullberg 1 876 from Shanxi and 1 1 species in the genus Tomocerus (s.s.; 4 from Tibet (monticolus, obsculus, parvus, and zayensis Huang and Yin 1981), 2 from Yunnan (varius Folsom 1899 andfolsomi Denis 1929), caputiviolaceus Lee 1975, cuspidatus Borner 1909 from Taiwan, kinoshitai Yosii 1954 from Hunan, ocreatus Denis 1948 from Zhejiang, and sibiricus Reuter 1891 from Hebei. A species of the subgenus: Tomocerus (s.s.) spinulus, is described here. Tomocerus (s.s.) spinulus, NEW SPECIES Color: background pale yellow with purplish blue pigment. Head with pale, scattered pigment on anterior margin between antennae. Eye patches dark. Lateral sides of Ant. I & II pale purplish blue; Ant. Ill gradually darker from base to apex. Ant. IV totally dark. Pattern as shown in Fig. 1 . Distal half of tibiotarsus with pale, scattered pigment. Head: Eyes 6+6, A & B larger, others subequal. Antennae up to 1 .3 mm long; ratio of antenna/ cephalic diagonal: 2.9/1 to 3.2/1; average segment ratios: 1-4= 1/1.2-1.6/6.67-7.67/1-1.4. Head capsule with 2,4 anterior setae and about 40 small setae in a transverse row at posterior margin (Fig. 2). Labral setae 4,5,5,4 with 4 marginal recurving spinules (Fig. 3). Body: Thorax with dorsal chaetotaxy and bothriotricha as in Fig. 4. Unguis rather slender; a pair of well developed pseudonychia, about 0.25-0.35 as long as inner edge of unguis; inner teeth 5- 6 on all 3 pairs of legs. Unguiculus lanceolate with 1 inner tooth. Tenent hair well developed and spatulate, about as long as inner edge of unguis (Fig. 5). Tibiotarsus with numerous pointed smooth setae in different sizes, and legs I-III respectively with 3-4, 4-6, 6-8 apically tapered spine-like setae on ventral side (Fig. 6). Trochanteral organ reduced with 1/1 smooth setae (Fig. 7). Abdominal segments 1-5 with dorsal chaetotaxy and bothriotricha as in Fig. 4. Tenaculum unsealed, corpus with 4-7(9)* smooth setae (Fig. 8). Ventral tube scaled, anteriorly with 1 8 smooth 1 Received March 15, 1997. Accepted May 15, 1997. 2 Department of Biology, Nanjing University, Nanjing 210093, P. R. China 3 Department of Biology, Grinnell College, Grinnell, I A 501 12, U. S. A. * Numbers in parentheses represent unusual conditions. ENT. NEWS 1 09( 1 ): 5 1 -55, January & February, 1 998 52 ENTOMOLOGICAL NEWS Tomocerus spinulus. All figures of type specimens. 1 . Habitus; 2. Head, 3. Labrum, 4. Chaetotaxy of body, 5. Hind claw, 6. Hind tibiotarsus, 7. Trochanteral organ, 8. Tenaculum, 9. Anterior face of ventral tube, 10. Lateral flap of ventral tube. Vol. 109, No. 1, January & February, 1998 53 15 17 16 a- Tomocerus spinulus. All figures of type specimens. 11. Distal part of manubrium (dorsal view), 12. Dental spines, 13. Single dental spine, 14. Mucro, 15. Upper anal valve of Abd. VI, 16. Microsetae at base of macrochaeta, 17. Bothriothrix. 54 ENTOMOLOGICAL NEWS setae (holotype) on each side (Fig. 9), posteriorly with numerous smooth setae in different sizes; lateral flap with about 60 smooth setae in different sizes (Fig. 10). Ratios of manubrium/dens/ mucro = 2. 3-3.0/3. 3-4.0/ 1 .0. Manubrium with a longitudinal band of lateral setae on each side and 2 longitudial bands of setae on dorsal side; lateral setae large, weakly ciliated, gradually tapering but more strongly tapering and pointed distally; each dorsal band of setae consists of numerous setae in different sizes, those in outer row and at distal part very large; all setae very weakly ciliate to striate and pointed; no blunt "principal setae" present; scales present between longitudinal bands of setae (Fig. 11). Dental spines dark chestnut brown, formula 4(3)/4(3)-5,I, each with many tiny teeth (spinules) (Fig. 1 2 & 13). Denies without inner differentiated swollen scales or outer spine-like setae. Mucro elongate, with numerous ciliate setae; outer dorsal lamella bearing 3-6 intermediate smaller teeth; outer basal tooth with a corner toothlet; apical and anteapical teeth subequal (Fig. 1 4). Dorsal anal valve of Abd. VI with 7 large setae arranged in an irregular transverse row (Fig. 15). Body scales brownish, hyaline and heavily striate. Each body macrochaeta surrounded by 0(1-2) setulae (Fig. 16). Bothriotricha not surrounded by setulae (Fig. 17). Size: Maximum body length 2.7 mm. Etymology: The name of this species is derived from the Latin spinula = spines. It refers to the numerous spinules on dental spines. Type materials. Holotype: male, China: Anhui Province, Yellow Mt., VII- 16- 1990, collection numbers 8220 & 8221. Jian Xiu Chen coll. Deposited in the Department of Biology, Nanjing University. Paratypes: 10 females & 4 males, same data as Holotype. DIAGNOSIS This species bears some similarity to the widespread T. ocreatus but can easily be distinguished by the much finer denticulations on the dental spines, the smaller number of tenacular setae and the unguis shape. It also resembles the Korean species jesonicus Yosii 1967 and spinistriatus Lee 1975 as well as the Tibetan species zayuensis Huang and Li 198 1 but may be readily separated as shown below: Character spinulus jesonicus spinistriatus zayuensis Dental spines 4(3)/4(3)-5,I 5-6/5-6,1,1,1 5-6,1/6-7,1 3,3/3-4,11 Tenaculum setae 4-7(9) 15 1 ? Unguiculus tooth + + Vol. 109, No. 1, January & February, 1998 55 Tomocerus spinulus* is much more similar to the Pakistan species T. asoka Yosii & Ashraf 1965 & Japanese species T. viridis Yosii 1967. It shares fea- tures such as the structure of claw and the number of dental spines; however, it differs from them in the body color and the features listed in the following table. SPECIES Character spinulus asoka viridis Spinules on dental spines tiny but numerous absent absent except on distalmost spine Thick spine-like setae 3-4, 4-6, 6-8 ? 5, 5, 5 on tibiotarsi Tenacular setae 4-7(9) 15 2 Maximum body length (in mm.) 2.7 5.0 2.0 * Found in leaf litter in a deciduous forest. ACKNOWLEDGMENTS We would like to thank Mr. Ma Yi-tong in the Department of Biology of Nanjing University, whose help was essential for this work. Publication of this work was made possible by a grant from Grinnell College. LITERATURE CITED Denis, J. R. 1929. Notes sur les Collemboles recoltes dans ses voyages par le Prof. F. Silvestri. I. Seconde note sur les Collemboles d'Extreme-Orient. Bull. Lab. Zool. Portici 22: 305-320. Denis, J. R. 1948. Collemboles d'Indochine recoltes de M. C. Dawydoff. Notes d'Entomologie Chinoise, Musee Heude, Vol XII, Fasc. 17: 183-259. Huang, F-S and H-F Yi. 1 98 1 . Insects of Xizang, Vol. 1 , pp. 41-46, China Science Press, Beijing. No. 13031.1657. In Chinese. Huang, Fusheng, 1 995. Three new recorded species of Tomocerus Nicolet in China (Collembola: Tomoceridae). Sinozoologia 12: 192-193. Lee, B.H. 1975 .Etude de la faune coreene des Insectes Collemboles VI. Sur la famille des Tomoceridae, edaphiques avec la description de quatre nouvelles especes et d'une nouvelle sous-espece. Bull Mus. natn. Histoire Natur. Paris 317: 945-961. Yosii, R. & Ashraf, M. 1965. On Some Collembola of West Pakistan. IV. Pakistan J. Sci. Res. 17: 153-160. Yosii, R. 1967. Studies on the Collembolan Family Tomoceridae, with Special Reference to Japanese Forms. Contrib. Biol. Lab. Kyoto Univ. No. 20, 1-54. Zhao, Lijun et al., 1 997. Tentative checklist of Collembolan species from China (Insecta). Publ. Itako Hydrobiol. Stn., 9: 15-40. 56 ENTOMOLOGICAL NEWS SPECIES DISTINCTION IN ABDOMINAL PIGMENTATION PATTERNS BETWEEN FEMALES OF DROSOPH1LA MELANOGASTER AND D. SIMULANS, FROM A SPANISH POPULATION1 Karel Th. Kisses^, Mauro Santos^ ABSTRACT : The sibling species Drosophila melanogaster and D. simulans coexist in natural conditions. Whereas males are easily recognizable by their genital arches, females were consid- ered to be indistinguishable but for their eye sizes. In many papers separate female counts were omitted because of this difficult characteristic. However, the abdominal pigmentation pattern was found to be different between the two species in a Spanish population. The discrimination of the females based on pigmentation differences was checked by electrophoresis and found to be very reliable. Since the discovery of Sturtevant (1919) that Drosophila melanogaster has a closely resembling sibling species D. simulans, both species are known to be cosmopolitan and coexistent (Lachaise et al., 1988). In some population screens the authors make no effort to distinguish the females of the two species, and only mention their grand total (Tantawy & Soliman, 1967; references in Lachaise et al., 1988). Most often, research starts with isofemale lines and checking their progeny in which the males of the two species are distinguishable due to differ- ent genital arches (Burla, 1951;Coyne, 1983; Sturtevant, 19 19). Based on mea- surements of eye sizes of D. melanogaster and D. simulans, it is possible to make a distinction between the females (Burla, 1951; Gallo, 1973; McNamee & Dytham, 1993) but it is a painstaking job when large numbers of flies have to be examined. Okada (1956) described a way of discrimination based on differ- ences in egg guides, but this character also necessitates much practice to distin- guish the two species. A high number (up to 45 %) of misqualifications of D. melanogaster have been reported, based on different eye size definitions (McNamee & Dytham, 1993 and references therein). We used flies captured in traps in Carboneras (Almeria, Spain) to see if a way of morphological distinction by abdominal pigmentation differences (Gallo, 1 973) might be applicable in our population of D. melanogaster and D. simulans. Eye size was used as the character to separate the species, but we also checked the pigmentation of the sixth tergite to see whether a useful correlation existed. 1 Received November 5, 1996. Accepted July 23, 1997. 2 Universitat Autonoma de Barcelona, Departament de Genetica i de Microbiologia, 08 193 Bellaterra (Barcelona), Spain. 3 Present address and address for correspondence: Utrecht University, Department of Plant Ecol- ogy and Evolutionary Biology, Padualaan 8, 3584 CH Utrecht, The Netherlands, fax: 31-30- 2513655, e-mail:kteisses@dds.nl ENT. NEWS 109(1): 56-60, January & February, 1998 Vol. 109, No. 1 , January & February, 1998 57 Females emerging from Opuntia ficus-indica fruits were primarily discrimi- nated by the pigmentation pattern of the sixth tergite. All flies deemed D. melanogaster were subjected to electrophoresis for other reasons (Hisses and Santos, 1997). MATERIALS AND METHODS Flies were captured with mashed banana traps during five days in the Carboneras area (Almeria, Spain; 37°00'N; 1°53'W) and locations nearby (Eisses and Santos, 1997). Opuntiaficus-indica fruits (prickly pears) were put in trays in a semi-abandoned O. ficus-indica plantation and left for almost seven days in the field. After recollection, the fruits were placed in glass jars, and emerging flies were aspirated. Captured and emerged flies were checked for D. melanogaster morphology and frozen at -29 °C until electrophoresis. ADH is a diagnostic enzyme between D. melanogaster and D. simulans because of clearly distinctive bands in gel electrophoresis (Eisses, Van Dijk & VanDelden, 1979). RESULTS The apparent D. melanogaster females trapped in the O. ficus-indica plan- tation near Carboneras were separated from D. simulans by eye size only, whereas flies from the other locations were separated at the species and sex level by eye size and genital arches. After electrophoresis the number of misqualifications of D. melanogaster was calculated (Table I A). Almost 21 % of the female flies turned out to be D. simulans (Table I A 1). For females and males together a general misqualification of 1 1 % was obtained (Table I A 2). Table I. Number of flies initially separated as D. melanogaster and percentage of misqualifications based on electrophoresis of flies trapped in banana baits in a semi abandoned O. ficus-indica plantation (A 1) and other locations close to Carboneras (A 2), and of flies emerging from O. ficus-indica fruits collected at the plantation (B). Method of distinction No. initially separated % actually as D. melanogaster determined as D. simulans 'A 1 Eye size 226 females 20.8 2 Eye size / Genital arch 437 females / males 1 1 .0 B Pigmentation of 6th tergite and eye size 1 078 females 1 .68 ± 0.26* Genital arch 1 092 males 0.64 ± 0.034 *Empirical Standard Deviation 58 ENTOMOLOGICAL NEWS Flies emerging from O. ficus-indica fruits were separated primarily by the morphological distinction of the pigmentation pattern of the sixth tergite (Fig. 1) and in cases of doubt the eye size was examined as well. Approximately equal numbers of female and male flies were checked by each of us. After checking the flies with electrophoresis, the average percentage of misquali- fications of the females was calculated to be 1 .68 % +.0.26. This is in the same order as misqualifying male flies (Table I B). The most important difference between D. melanogaster and D. simulans females is the black pigmentation of the sixth tergite, which runs to the ventral margin in D. melanogaster, whereas the pigmentation border line in D. simulans makes an angle with the tergite margin. It forms a continuous line with the pigmentation border line in the seventh tergite (Fig. 1 a). In contrast with an apparently monomorphic D. simulans, we observed large variation in abdomi- nal pigmentation patterns in this natural population of D. melanogaster and also in some laboratory strains (Fig. 1 b - i). g Figure 1 . Pigmentation patterns of the 6th and 7th tergite of D. simulans (a) and D. melanogaster (b - i). Within D. melanogaster variation was present in wild type populations and in homozy- gous or isogenic laboratory strains Groningen SSN (b) and Groningen FFF (d). None of the D. melanogaster strains was monomorphic. Vol. 109, No. 1, January & February, 1998 59 We have demonstrated that distinction between females of a natural popu- lation of D. melanogaster and D. simulans in Spain can be made in an easy and reliable way. As similar observations have been made on females from a Brazil- ian population (Gallo, 1973) and from a midwestern U.S. population (Thomp- son, Hisey & Woodruff, 1979) it might be generalized to more populations of D. simulans. It seems worthwhile to excavate information about other D. simulans populations with respect to female abdominal pigmentation of the sixth tergite to establish whether or not D. simulans is world wide monomor- phic for this character in contrast to D. melanogaster (Robertson, Briscoe & Louw, 1977; David, Capy & Gauthier, 1990). Robertson, Briscoe and Louw (1977) described the focus fap (female abdomen pattern) to be residing on the extreme tip of the 3L chromosome, with some effects from the fourth chromo- some. This might be the reason why the D. melanogaster Groningen-FFF strain, used as a reference in electrophoresis, showed a pigmentation pattern in the sixth tergite like D. simulans (Fig. 1 d). This strain is partly homozygous for the second chromosome and the third chromosome. ACKNOWLEDGMENTS Montse Peiro is thanked for helping with electrophoreses of the flies and Albert Kamping (University of Groningen) for providing the Groningen reference strains of Drosophila melanogaster. Prof. Wim Scharloo and Dr. Agusti Galiana are thanked for their comments on an earlier draft. This work was funded by Contract No. CHRX-CT92-0041 from the Commission of the European Communities, and grant PB-0843 from the DGICYT (Spain) to A. Eontdevila. LITERATURE CITED Burla, H. 1 95 1 . Systematik, Verbreitung und Oekologie der Drosophila-Arten der Schweiz. Rev. SuisseZool. 58, 23-175. Coyne, J.A. 1983. Genetic basis of difference in genital morphology among three sibling species of Drosophila. Evolution 37, 1101-1117. David, J.R., Capy, P., & Gauthier, J.-P. 1990. Abdominal pigmentation and growth tempera- ture in Drosophila melanogaster: Similarities and differences in the norms of reaction of successive segments. J. Evol. Biol. 3, 429-445. Kisses, K.Th., & Santos, M. 1 997. Short distance differences in ADH and alphaGPDH allozyme polymorphisms and linkage disequilibrium in D. melanogaster in Spanish desert popula- tions. Dros. Inf. Serv. 80: in press. Kisses, K.Th., van Dijk, H. & van Delden, W. 1979. Genetic differentiation within the Melanogaster species group of the genus Drosophila (Sophophora). Evolution 33,1 063- 1 068. Gallo, A J. 1 973. Morphological distinction between female Drosophila melanogaster and fe- male D. simulans. Ciencia & Cultura 25, 341-345. Lachaise, D., Cariou, M.-L., David, J.R., Lemeunier, F., Tsacas, L. & Ashburner, M. 1988. Historical biogeography of the Drosophila melanogaster species group. Evol. Biol. 22, 159-225. 60 ENTOMOLOGICAL NEWS McNamee, S. & Dytham, C. 1993. Morphometric discrimination of the sibling species Droso- phila melanogaster (Meigen) and D. simulans (Sturtevant) (Diptera: Drosophilidae). Syst. Entomol. 18,231-236. Okada, T. 1956. Systematic study of Drosophilidae and allied families of Japan. Gihodo Co., Tokyo, Japan. Robertson, A., Briscoe, D.A. & Louw, J.H. 1977. Variation in abdomen in Drosophila melanogaster females. Genetica 47, 73-76. Sturtevant, A.H. 1919. A new species closely resembling Drosophila melanogaster. Psyche 26, 153-156. Tantawy, A.O. & Soliman, M.H. 1967. Studies on natural populations of Drosophila. VI. Com- petition between Drosophila melanogaster and Drosophila simulans. Evolution 21, 34-40. Thompson Jr., J.N., Hisey, B.N. & Woodruff, R.C. 1979. Morphological differences between the females of Drosophila melanogaster and Drosophila simulans. Southwest Nat. 24, 204-205. CURTIS W. SABROSKY The American Entomological Society deeply regrets the recent passing of Dr. Curtis W. Sabrosky, a friend and Honorary Member of the Society. Although a long time member of the Entomologi- cal Society of Washington, DC, in recent years, following his move to Medford Leas, Medford, NJ, Curtis regularly attended meetings of the American Entomological Society. Because Curtis was honored in 1982 in a "festschrift" (Vol. 10) edition of the Memoirs of the Entomological Society of Washington, only a brief notice is now planned by the Wash- ington Society, together with publication of a complete bibliography of his entomological contributions. -H.P.B Vol. 1 09, No. 1 , January & February, 1 998 61 SYNONYMIC NOTES ON SOME OF THOMSON'S NEW WORLD DOLICHOPODIDAE (DIPTERA)1 Daniel J. Bickel ' ABSTRACT: Types of four New World Dolichopodidae described by C.G. Thomson ( 1 869) from material gathered during the voyage of the Swedish frigate Eugenie were examined. Three new synonyms are established. Dolichopus lamellicornis is a senior synonym of Hygroceleuthus afflictus. Of three overlooked species described by Thomson from Puna, Ecuador, Chrysotus ochropus is redescribed and regarded as a senior synonym of C. flavipalpus, Psilopus zonatulus is a junior synonym of Condylostylus longicornis, and Psilopus pleuralis, known only from the female type, is referred to the Condylostylus caudatus group. While completing a faunal study of the Galapagos Dolichopodidae (Bickel & Sinclair, in press), I had the opportunity to examine some New World dolichopodid types described by C.G. Thomson, 1869 from material gathered during the voyage of the Swedish frigate Eugenie (see Persson, 1 97 1 for an au- thoritative account of the collecting localities). Some of these species had been overlooked in revisions of New World dolichopodid genera, and/or had been omitted from major catalogues. Three of the species proved to be synonyms. These matters are discussed below. Dolichopus lamellicornis Thomson. Dolichopus lamellicornis Thomson, 1 869: 511. Hygroceleuthus afflictus Osten Sacken 1877: 313. NEW SYNONYM. Dolichopus lamellicornis was overlooked in the principal revision of North American Dolichopus (Van Duzee, et al., 1921) and subsequently was listed under "Unplaced Species of Dolichopodidae" in the Catalog of the Diptera of America North of Mexico (Foote, et al., 1965). The lectotype of Dolichopus lamellicornis, here designated, is a female, bearing the labels: "California/ Kinb./Type". The body length of the lectotype is 6.0 mm, not 7.0 mm as stated in Thomson's description. I used the key to female Nearctic Dolichopus (Van Duzee & Curran, 1934) and the specimen was confidently identified as the widespread western North American Dolichopus afflictus (Osten Sacken), an identification substantiated by the description in Van Duzee, et al., 1921. Although the lectotype of 1 Received April 14, 1997. Accepted May 3, 1997. 2 Entomology Section, Australian Museum, 6 College Street, Sydney, NSW 2000 AUSTRALIA, e-mail: danb@amsg.austmus.oz.au ENT. NEWS 109(1): 61-65, January & February, 1998 62 ENTOMOLOGICAL NEWS Dolichopus lamellicornis bears a locality label with only the word "Califor- nia", the ship's records note that the only Californian port of call was San Francisco, from July 3 to August 11, 1852, and the specimen probably was collected in vicinity of that city. The type locality of D. afflictus is San Rafael, Marin County, adjoining San Francisco Bay. Osten Sacken 1877, in his description of Hygroceleuthus afflictus (Hygro- celeuthus is a synonym of Dolichopus) thought that on the basis of Thomson's description, D. lamellicornis was possibly the female of the species. He rea- soned that if Thomson's female specimen had an indentation on hind wing mar- gin similar to that of male D. afflictus, then the two species possibly were conspecific, especially since both sexes of the related species D. crenatus had indented hind wing margins. Since Thomson's description did not note any wing indentation, Osten Sacken therefore considered them to be different spe- cies. Therefore, it is noted here that the wing of the Dolichopus lamellicornis female lectotype does have a marginal indentation at the apex of vein CuA, as has been described for female D. afflictus. The following three species were collected at Puna, an island in the Gulf of Guayaquil, Ecuador, and are not listed in the "Catalogue of the Diptera of the Americas south of the United States" (Robinson, 1970), nor in any other re- cent Diptera catalog. Becker (1922a: 220) thought that the locality "Puna" given in Thomson's descriptions was Puma (sic.), India, and therefore he listed one of the species, Psilopus zonatulus (as Condylostylus zonatulus) in his mono- graph on the Indo-australian fauna. None of the three species appears in his treatment of the New World Dolichopodidae (Becker, 1922b). Chrysotus ochropus Thomson. Chrysotus ochropus Thomson, 1 869: 505. Chrysotus flavipalpus Van Duzee, 1930: 76. NEW SYNONYM. The lectotype, here designated, is a male in fair condition bearing the la- bels "Puna/ Kinb./Type". A diagnosis based on the holotype is given below, and the species is placed in synonymy with Chrysotus flavipalpus Van Duzee, described from San Bartolome, Lima, Peru. In the following diagnosis, the relative lengths of the podomeres are representative ratios, not measurements, and for each leg are given in the following formula and punctuation: trochanter + femur; tibia; tarsomere 1/2/3/4/5; the abbreviations (MSSC) means "male secondary sexual character(s)," the non-genitalic characters found only on the male body. Diagnosis: Male: length: 2.0 mm; wing: 1.7 x 0.6 mm. Head: vertex and frons metallic blue-green with some grey pruinosity; pairs of strong diverging ocellars, strong verticals, and short postverticals present; eyes almost joined across face-clypeus, but separated by narrow band Vol. 109, No. 1, January & February, 1998 63 of grey pruinose cuticle; palp yellow; proboscis brown; scape and most of pedicel yellow; distalmost pedicel and first flagellomere brown; first flagellomere subtriangular with apical arista; ventral postcranium with pale postorbitals. Thorax: mostly obliterated by pin, but metallic green with bronze reflec- tions; setae black; lateral scutellar setae about 1/4 the length of medians. Legs: coxa I yellow; coxae II and III brown; remainder of legs mostly yellow; coxae I and II with pale anterior setae; coxa III with strong brownish lateral seta; leg I: 2.7; 2.3; 1 .0/ 0.6/ 0.3/ 0.2/ 0.3; femur I in distal half with row of 7 projecting brown pv setae (MSSC); tibia I with crestlike row 12-15 pale ventral hairs which are longer than normal vestiture, and which continue as row of short pale hairs on tarsus I (MSSC); leg II: 3.2; 2.8; 1.2/ 0.5/ 0.4/ 0.3/ 0.3; femur II with group of 4-5 brown subapical pv setae (MSSC?); tibia II with very strong ad and much weaker pd near 1/4, and with weak offset ad and pd setae near 1/2, and with strong ventroapical seta; leg III: 3.5; 3.7; l.O/ 0.8/ 0.4/ 0.3/ 0.3; femur III with 3-4 long brownish av and pv setae from 1/2 to 3/4 (MSSC), and in apical quarter with very strong av seta followed distally by weaker seta; tibia III with ad and pd setal pairs at 1/5 and 1/2, and with some short dorsal setae. Wing: hyaline; 1*4+5 and M parallel to apex; CuAx ratio: 0.3; lower calypter yellow with fan of brownish setae; halter yellow. Abdomen: metallic blue-green with bronze reflections, and covered with short brownish vestiture; hypopygium with dark brown capsule and yellow cercus. Remarks: Chrysotus ochropus is now known from coastal Ecuador and Peru. It is closely related to C. brevicornis Van Duzee, found in the Galapagos, Central America and Mexico. Both species have a similar overall description and coloration, and have similar leg setation: pv setae on femur I (MSSC), tibia I with row of pale ventral hairs (MSSC), similar tibia II setation, femur III with long brownish pv setae from 1/2 to 3/4 (MSSC). They belong in the picticornis group (see Bickel & Sinclair, in press, for further discussion). Condylostylus longicornis (Fabricius) Musca longicornis Fabricius, 1775: 783. Psilopus zonatulus Thomson, 1 869: 509. NEW SYNONYM Condylostylus zonatulus (Thomson) [Becker, 1922a: 220]. The lectotype of Psilopus zonatulus, here designated, is a female bearing the labels "Puna/ Kinb./Type". It is conspecific with Condylostylus longicornis (Fabricius), agreeing in every detail with this common and widespread New World species. The earlier synonyms and distribution of this species are dis- cussed in Bickel (1994: 108). 64 ENTOMOLOGICAL NEWS It should be noted that Psilopus zonatulus Thomson is also a junior hom- onym of the Palearctic Psilopus zonatulus Zetterstedt, 1843, a valid species in the genus Sciapus (see Meuffels & Grootaert, 1990). Condylostylus pleuralis (Thomson). Psilopus pleuralis Thomson, 1869: 510. Psilopus pleuralis is known only from the female lectotype, here desig- nated, which bears the labels the labels "Puna/ Kinb./ Type". It was referred to Condylostylus in Bickel (1994: 107). This species clearly belongs in the widespread New World group of spe- cies centered around Condylostylus caudatus (Wiedemann) (see Becker, 1922b and Van Duzee, 1927). However, females in this group cannot be accurately identified. Previously, C. caudatus itse\f was thought to be widespread through- out both North and South America, but both Van Duzee (1927) and Robinson ( 1 970, 1 975) regard most of the tropical records as being that of C. graenicheri (Van Duzee) or some other related species. Without further study, it is prema- ture to make a decision, but C. pleuralis is possibly a senior synonym of C. graenicheri (Van Duzee) or C. barbatus (Aldrich), or a junior synonym of two other South American caudatus group species, C. anceps (Wiedemann) and C. smaragdulus (Wiedemann). Unfortunately, both Wiedemann species are described from females and remain unidentifiable. Until the entire New World caudatus group is revised, there will be uncertainty regarding names. The large number of old species based on unidentifiable females is regrettable, espe- cially since they have the potential to become senior synonyms of subsequent well-described species. ACKNOWLEDGMENTS I thank Bert Viklund of the Naturhistorika Riksmuseet, Stockholm, for kindly making the Thomson material available to me. F.C. Thompson, United States National Museum, Washing- ton, D.C., and C.E. Dyte, University of Reading, England, refereed the manuscript and provided valuable comments. LITERATURE CITED Becker, T. 1922a. Dipterologische Studien, Dolichopodidae der indo-australischen Region. Capita Zoologica 1(4): 1-247. Becker, T. 1922b. Dipterologische Studien. Dolichopodidae. B. Nearktische und neotropische Region. Abhandlungen der Zoologische-Botanische Gesellschaft in Wien (1921) 13(1): 1- 394. Bickel, D.J. 1994. The Australian Sciapodinae (Diptera: Dolichopodidae), with a review of the Australasian and Oriental faunas, and a world conspectus of the subfamily. Records of the Australian Museum , Supplement 21: 1-394. Vol. 109, No. 1, January & February, 1998 65 Bickel, D.J. & B.J. Sinclair, (in press). The Dolichopodidae (Diptera) of the Galapagos Islands, with notes on the New World fauna. Entomologica Scandinavica. Fabric ins, J.C. 1775. Systema entomologiae. 832pp. Flensburgi et Lipsiae. Foote, R.H., J.R. Coulson, & H. Robinson 1965. Family Dolichopodidae, p. 482-530, In: A. Stone et al., eds. A Catalog of the Diptera of America North of Mexico. United States Department of Agriculture, Agricultural Handbook 276, 1696 pp. Meuffels, H.J.G. & P. Grootaert. 1 990. The identity of Sciapus contristans (Wiedemann, 1817) (Diptera: Dolichopodidae), and a revision of the species group of its relatives. Bulletin de 1'Institut Royal des Sciences Naturelles de Belgique 60: 161-178. Osten Sacken, C.R. 1877. Western Diptera: descriptions of new genera and species of Diptera from the region west of the Mississippi and especially from California. Bulletin of the United States Geological and Geographical Survey of the Territories. 3: 189-354. Persson, P.I. 1971. "Eugenics resa". Localities, dates and labels of the insects collected during the voyage around the world by the Swedish frigate "Eugenie" in the years 1851-1853. Entomologisk Tidskrift 92: 1 64- 1 72. Robinson, H. 1970. Family Dolichopodidae. In: N. Papavero, ed. A catalogue of the Diptera of the Americas south of the United States. 40, 92 pp. Museu de Zoologia, Universidade Sao Paulo, Brasil. Robinson, H. 1975. Bredin-Archbold-Smithsonian biological survey of Dominica. The family Dolichopodidae with some related Antillean and Panamanian species. Smithsonian Contri- butions to Zoology 185: 1-141. Thomson, C.G. 1 869. Diptera, pp. 443-61 4, In: Kongliga svenska fregatten Eugenics resa omkring jorden under befal af C. A. Virgin, aren 1851-1853. 2 (Zoologi), I (Insekter), 617 pp., Stockholm. Van Duzee, M.C. 1927 .Three new species ofPsilopus from North America, and notes on caudatus Wied. (Dipt.: Dolichopodidae). Entomological News 38: 72-76. Van Duzee, M.C. 1930. Dolichopodidae, Part 5(1), Pp. 1-92, pis. 1-3. In: Diptera of Patagonia and South Chile. British Museum (Natural History), London. Van Duzee, M.C., F.R. Cole, & J.M. Aldrich. 1921 . The dipterous genus Dolichopus Latreille in North America. Bulletin of the United States National Museum. 1 16, 304 pp. Van Duzee, M.C. & C.H. Curran. 1934. Key to the females of Nearctic Dolichopus Latreille (Diptera). American Museum Novitates 684, 17 pp. 66 ENTOMOLOGICAL NEWS DRY WEIGHT OF FRESH AND PRESERVED SPIDERS (ARANEIDA: LABIDOGNATHA)1 Robert L. Edwards2, Wendy L. Gabriel3 ABSTRACT: Data on the dry weight for 1 9 taxa (suborder Labidognatha) of fresh and preserved spiders are presented. The variation in weight at length for individual species is also provided. With the notable exception of the genus Tetragnatha, Family Tetragnathidae, the Family Theridi- idae, and most of the genera of the Family Thomisidae, the families examined are similar to one another in their weight-length relationships and are not readily separable on that basis. Dry weight can be approximated using either the weight of fresh specimens or preserved material. The 'typical' spider, based on the material examined, increases in length faster relatively than it increases in weight, and dry weight decreases relative to fresh weight as length increases. With the exception of the papers by Clausen, 1983, that included data on fresh and dry weight-length relationships for nine species (five families), and Breymeyer, 1967, for three species of the family Lycosidae, there is very little general information available on the dry weight of spiders. This report serves to increase the information available on spider weight and explores the degree of difference between taxa from the weight-length perspective. MATERIAL AND METHODS The bulk of preserved material was collected in 1989 and 1990 in the Frances Crane Wildlife Management area, Hatchville, Falmouth Township, Barnstable County (Cape Cod), Massachusetts, in connection with another study (Edwards 1993). Collection details are provided therein. All were preserved in 75% denatured ethanol and all had their alcohol replaced at least once, typically within 48 hours of collection. The total length was measured from the clypeus to the distal end of the abdomen using an ocular micrometer for specimens < 1 2 mm in total length and vernier calipers for those > 1 2 mm. The total length, as described above, was measured to the nearest 0.1 mm, and the specimens damp dried on absorbent paper before weighing. Obviously distorted speci- mens were not used. In those cases where the pedicel had elongated, the sepa- ration of the thorax from the abdomen was measured and the total measure- ment corrected accordingly. The fresh material for this study was collected in the months of June through September, 1996, from the same area and habitats as the preserved material with one exception. The collection of Leucauge regnyi Simon, Family Tetra- gnathidae, was made in Puerto Rico in September, 1996. All collections were 1 Received April 9, 1997. Accepted July 7, 1997. 2 Research Associate, United States National Museum, Box 505, Woods Hole, Ma. 02543 3 Wendy L. Gabriel, Northeast Fisheries Center, 166 Water St., Woods Hole, Ma. 02543 ENT. NEWS 109(1): 66-74, January & February, 1998 Vol. 109, No. 1, January & February, 1998 67 made in the afternoon, the spiders were immobilized in an ethyl acetate col- lecting jar, identified and measured that day, refrigerated overnight at 3° C, and weighed the following day on a Mettler A200 balance, accurate to 1 mg. Following this, the material was oven-dried at a temperature of 40° C for seven days. To check the efficacy of the drying regime, three samples of 50 or more mixed species samples were dried for an additional seven days, with the great- est additional loss of weight observed of less than 3% in all cases. Specimens of all species used in this study have been deposited in the United States Na- tional Museum. RESULTS AND DISCUSSION The families, genera, number of individuals weighed, and the range of total lengths are provided in Table 1 . A total of 2,315 measurements of fresh, dry fresh, and preserved specimens representing 78 genera and 17 families were made (Table 1). Note in Fig. 1 that the various taxa are identified with the first four letters of the taxon as listed in Table 1 . DC O O O O 0.25 0.2- o.i5- t o.H Q 0.05- • FRESH O DRY FRESH -f DRY PRESERVED n a a a a a + a a a a 0 THOMTMAR LINY LEUC ARAN LYCO SALT PISA PHOL TETR THEROXYO PHIL ANYPAMAUCLUB CORI AGEL GNAP TAXA Figure 1 . Condition factors for the taxa listed in Table 1 , using the equation k = (aLh) I L3. The taxa are identified using the first four letters of each taxon. There are values for each treatment (fresh, dry fresh, dry preserved) with the exception of AMAU, TMAR, PHOL, and OXYO, for which there was no dry preserved data available. 68 ENTOMOLOGICAL NEWS The log transformed least squares equation, In weight = lna + b(ln length), was used to estimate weight (mg) at length (mm). The statistical parameters are provided in Table 2. The coefficient of determination (r2) ranged from 0.743 (Oxyopidae, dry fresh) to 0.985 (Corinnidae, fresh). Averages, mini- mum, and maximum values are listed for all parameters. The average values for coefficient In a (intercept) varied considerably and were greatest (-1.976) for fresh and least for dry preserved specimens (-3.580), while the average values for exponent b (slope) varied relatively little, from 2.739 for dry preserved to 2.800 for dry fresh specimens (Table 2). The number of individuals and genera obtained for each family varied con- siderably (Table 1). It is clear from the statistical parameters presented in Table 2 that there was little difference in the weight-length relationships be- tween taxa. To compare weight at length using different taxa, while taking into consideration the differing length ranges over which the parameters were estimated, we calculated the condition factor for each taxon, k = (aLb) I L3 (L = mid-point of lengths (mm) in sample, a = Exponent In a) and the results shown in Fig. 1 . Of the 1 9 taxa the genus Tetragnatha Latreille (Tetragnathidae), the family Theridiidae, and the rotund crab-like members of the family Thomisidae (genera Xysticus C. L. Koch, Ozyptila Simon, Misumena Latreille, Misumenops F.O.P.-Cambridge, and Misumenoides F.O.P.-Cambridge) stand apart from the rest. The genus Tmarus Simon, family Thomisidae, is rela- tively slender (less crab-like and rotund), differing in this respect from the other genera of the family Thomisidae listed above and is plotted separately (TMAR). Similarly, the less elongate genera Leucauge White, and Pachygnatha Sundevall, family Tetragnathidae, differ in body form from the elongate mem- bers of the genus Tetragnatha and are also plotted separately (LEUC). The uniqueness of Tetragnatha was noted by Greenstone, et. al. 1985. Breymeyer (1967) in a study concerning the dry weight of preserved spi- ders reported that alcohol dissolves and extracts some parts of spider bodies. This appears to be the case in this study as well. It is worth noting that undried specimens preserved in denatured alcohol weigh considerably more than fresh material (Edwards 1996). Clausen (1983, p. 143-144) noted that "the ratio of dry over wetweight increases with decreasing size of specimens", and sug- gested that "With decreasing size, the exocuticle may make up a relatively greater part of the animal's weight because of the relatively greater surface. And, there may be a minimum thickness of the cuticle, which, in effect, will give the same result." To test Clausen's (op. cit.) suggestion further, the entire data set available was examined by treatment, i.e. fresh, dry fresh and dry preserved. The statistical parameters for each treatment are provided in Table 3. The percent of fresh weight for dry fresh and dry preserved material is shown in Fig. 2. The results are consistent, for the average spider, with the observa- tions of Clausen (op. cit.), with the caveat that more than the cuticle is un- Vol. 109, No. 1, January & February, 1998 69 y 0.35 LJJ X CO LJJ DC LJJ o DC LJJ Q. 0.25- 0.2- 0.15 0 10 15 LENGTH (MM) 20 DRY FRESH DRY PRESERVED Figure 2. Percent of fresh weight represented by dry fresh weight and dry preserved weight, based on average data for each treatment (Table 3). doubtedly involved since drying does not reduce the specimens to the cu- ticle only. Other tissues are involved which may also vary in the degree to which they are present in different taxa. The variability seen in individual species in the ratio of dry weight to fresh weight was examined for 1 1 species collected in October and November, 1 996. The individual species collections were each made within an hour in restricted localities to reduce environmental variabilities as much as possible. The col- lections were treated and analyzed as described earlier (see Table 4). On aver- age the ratio of dry to fresh weight was 0.314 ± 0.038, varying from 0.250 to 0.390. The average slope (b) of dry on fresh weight was slightly in excess of 1 with two notable exceptions, that of Pardosa lapidicina (b = 1 .224) and Phidippus clarus (b = 1 .537). The average intercept (In a) values for these two species were also well in excess of the average value, -2.015 and -3.161 re- spectively. Both of these collections were of immature individuals that would have matured the following year, although the Tmarus angulatus collection, also of immature individuals due to mature the following year, did not have a similar departure from the average values. The average r2 for the weight- length regressions of these species was 0.892 for fresh and 0.864 for dry fresh. 70 ENTOMOLOGICAL NEWS For studies requiring precision, e.g. those of a single or a set of closely related species, it would be best to use a sclerotized part of the body such as the head capsule, to reduce the problems associated with measurement error (cf. Jocque 1981). Whatever method is used, it is obvious that the weight of individual spiders is highly variable. In one survey (Edwards 1993) over 12,000 specimens were collected. Many of these were archived against the future. It was encouraging to find out that preserved material also served the purpose of realistically estimating dry weight. Table 1 . Fresh and preserved spiders examined. Number of individuals = n, number of genera - genera, lengths (mm) included in sample - range. Family Tetragnathidae is subdivided into the genus Tetragnatha and a second category of rounder body forms, including the genera Leucauge and Pachygnatha, listed as Leucauge. The family Thomisidae includes all genera sampled except the genus Tmarus which is listed separately. Fresh Fresh, dry Preserved, dry Taxon n genera range n genera range n genera range Agelenidae 66 5 4.5 - 19.1 29 1 7.4 - 19.1 52 3 3.7- 16.5 Amaurobiidae 27 1 4.0 - 14.1 26 1 4.0 - 14.1 Anyphaenidae 28 4 2.7 - 7.6 31 4 3.2 - 7.8 33 3 3.6- 9.2 Araneidae 90 4 2.7 -21.2 84 2.7 -20.5 56 9 2.3- 14.8 Clubionidae 30 4 2.3 - 8.8 26 3 2.5 - 11.1 19 5 2.0- 9.0 Corinnidae 20 1 2.2 - 8.6 10 1 3.2 -11.2 19 1 2.3- 7.1 Gnaphosidae 82 5 2.8 - 10.1 43 5 3.4 - 9.4 34 5 3.2- 11.6 Linyphiidae 60 9 1.5 - 5.5 43 5 2.5 - 5.4 56 9 2.0- 6.5 Lycosidae 92 11 1.5 - 16.8 85 9 4.0 - 16.8 53 11 2.6- 13.5 Oxyopidae 23 1 4.2 - 7.3 42 1 4.2 - 7.5 Philodromidae 25 3 2.0 - 9.0 31 3 2.9 - 12.5 39 3 2.0- 6.6 Pisauridae 16 2 5.5 - 19.3 16 2 4.0 - 11.1 25 1 2.1 - 12.0 Pholcidae 26 1 2.3 - 8.5 26 1 2.3 - 10.8 Salticidae 83 6 2.3 - 10.1 86 9 3.4 - 10.8 49 6 2.2- 9.0 Tetragnathidae Tetragnatha 58 1 2.5 - 11.0 42 1 3.0 - 10.2 31 1 2.4- 8.5 Leucauge 49 2 2.5 - 7.6 52 2 2.8 - 7.6 32 2 1.5- 7.1 Theridiidae 73 7 1.5 - 8.3 55 8 3.0 - 8.2 40 5 1.7- 6.3 Thomisidae 52 5 1.9 - 8.6 52 3 2.6 - 8.2 41 4 1.6- 9.1 Tmarus 28 1 4.3 - 6.4 29 1 3.0 - 8.2 Totals 928 78 808 69 579 68 Vol. 109, No. 1, January & February, 1998 71 Table 2. Statistical parameters for spider weight-length equations (In weight ug = In a + b (In length mm), for fresh, dry fresh and dry preserved material. No. of individuals - n, standard error - SE., coefficient of determination - r% exponent of In a = Exp In a. Fresh specimens Taxon n a±SE &±SE r2 Exp In a Agelenidae Amaurobiidae 66 27 -1.657 ±0.325 -2.303 ±0.1 50 2.553 ±0.1 13 2.999 + 0.077 0.888 0.984 0.191 0.100 Anyphaenidae Araneidae 28 90 -1.697 ±0.186 -1.726 ±0.374 2.514 + 0.116 2.746 ± 0.066 0.948 0.952 0.183 0.178 Clubionidae 30 - .928 + 0.212 2.636 ±0.1 13 0.951 0.145 Corinnidae 20 -2.002 ±0.1 11 2.595 ± 0.074 0.985 0.135 Gnaphosidae Linyphiidae Lycosidae Oxyopidae Pisauridae 82 60 92 23 16 -2.492 ± 0.237 -2.766 ± 0.260 -1.746 + 0.277 -1.706±0.147 -2.963 ± 0.207 2.930 + 0.098 2.647 + 0.108 2.695 ± 0.080 2.571 +0.209 3.272 + 0.177 0.918 0.919 0.926 0.878 0.961 0.083 0.171 0.174 0.182 0.052 Philodromidae 25 -1.707 ±0.233 2.740 ±0.1 35 0.947 0.181 Pholcidae 26 -2.453 ±0.1 54 2.905 ± 0.076 0.984 0.086 Salticidae 83 -2.403 ± 0.248 3.027 ±0.071 0.957 0.090 Tetragnathidae Tetragnatha Leucauge Theridiidae 58 49 73 -2.268 ± 0.240 - .966 + 0.237 - .456 ±0.222 2.431 ±0.113 2.853 ±0.1 27 2.839 ±0.071 0.892 0.915 0.958 0.103 0.140 0.233 Thomisidae 52 - .447 + 0.199 2.945 ± 0.086 0.959 0.229 Tmarus 28 - .860 ±0.108 2.743 + 0.177 0.903 0.156 Average Minimum - .976 ±0.199 -2.963 ±0.1 08 2.771 ±0.1 10 2.431 ±0.066 0.938 0.878 0.148 0.052 Maximum -1.447 ±0.374 3.272 + 0.209 0.985 0.233 Dry fresh specimens Agelenidae Amaurobiidae 29 26 -4.504 ±0.326 -4.045 + 0.221 3.184 + 0.308 3.198±0.119 0.798 0.968 0.011 0.018 Anyphaenidae Araneidae 31 84 -2.652 ± 0.23 1 -2.401 +0.368 2.406 ±0.2 14 2.61 5 ±0.077 0.813 0.934 0.070 0.091 Clubionidae 26 -3.722 ± 0.265 2.999 ±0.1 39 0.951 0.024 Corinnidae 10 -3.896 ±0.1 65 3.054 + 0.175 0.975 0.020 Gnaphosidae Linyphiidae Lycosidae Oxyopidae Pisauridae 43 43 83 42 16 -3.584 ± 0.329 -2.761 ±0.260 -3.253 ±0.271 -3.473 ±0.269 -3.107 + 0.178 2.845 ±0.1 98 2.530 ± 0.209 2.804 ± 0.093 2.905 + 0.270 2.743 + 0.182 0.835 0.830 0.917 0.743 0.942 0.028 0.060 0.039 0.03 1 0.045 Philodromidae 31 -2.643 + 0.388 2.617 ±0.196 0.860 0.071 Pholcidae 26 -3.460 + 0.462 3.354 ±0.181 0.953 0.014 Salticidae 86 -3.330 ±0.289 2.904 ±0.1 20 0.875 0.036 Tetragnathidae Tetragnatha Leucauge 42 52 -2.350 ± 0.285 -3.253 ±0.272 1.914±0.158 2.920 ±0.1 63 0.785 0.866 0.095 0.039 72 ENTOMOLOGICAL NEWS 0 Taxon n a + SE b±SE r2 Exp In a Theridiidae 55 -3.436 ±0.3 17 3.22910.177 0.863 0.032 Thomisidae 52 -2.41410.329 2.741 10.147 0.874 0.089 Tmarus 29 -3.043 ± 0.203 2.790 + 0.195 0.884 0.048 Average Minimum -3.22810.278 -4.50410.165 2.829 + 0.175 1.91410.077 0.871 0.743 0.046 0.011 Maximum -2.35010.388 3.229 + 0.308 0.975 0.095 Dry preserved specimens Agelenidae 52 Anyphaenidae 33 Araneidae 56 -5.38010.375 -3.284 1 0.267 -3.607 1 0.484 3.38610.145 2.482 1 0.224 3.00410.138 0.916 0.798 0.898 0.005 0.037 0.027 Clubionidae 19 -3.35610.253 2.45810.146 0.943 0.035 Corinnidae 19 -2.946 1 0.222 2.46310.122 0.960 0.053 Gnaphosidae 34 Linyphiidae 56 Lycosidae 53 Pisauridae 25 -4.380 1 0.297 -3.308+0.288 -3.54210.294 -3.64310.307 3.011 +0.193 2.64610.120 2.772 1 0.088 2.74410.101 0.883 0.900 0.951 0.970 0.053 0.037 0.029 0.026 Philodromidae 39 -2.801 10.363 2.474 + 0.213 0.784 0.061 Salticidae 49 -4.13910.280 3.10910.124 0.931 0.016 Tetragnathidae Tetragnatha 3 1 Leucauge 32 Theridiidae 40 -3.59010.371 -3.58910.378 -2.952 1 0.280 2.182 + 0.181 2.79610.183 2.553 + 0.133 0.833 0.886 0.906 0.028 0.028 0.052 Thomisidae 41 -3.18410.210 3.001 10.087 0.968 0.041 Average Minimum -3.58010.311 -5.38010.210 2.73910.147 2.18210.087 0.902 0.784 0.032 0.005 Maximum -2.801 +0.484 3.386 1 0.224 0.970 0.061 Table 3. Statistical parameters for spider weight-length equations for treatment. Equation and table headings as in Table 2. all available material by Treatment n In a ± SE b± SE r2 Exp. In a Fresh 928 -1.87410.447 2.73310.031 0.891 0.153 Dry fresh 808 -2.857 1 0.436 2.637 1 0.039 0.847 0.057 Dry preserved 579 -3.27910.537 2.581 10.046 0.829 0.038 Vol. 109, No. 1, January & February, 1998 73 •s g -^ 04 •t V "& P •" .P Tt U 2 5 g si-S a ^ oo ON | 00 so 04 oo ON oo Os oo ON •n Os m ON r~- ON O4 SO t- sO OS ON .5: i*. to O o o 0 O O O O o o O 0 .— O _ n A 49 _ rn Os Tt" OO n-> f^. oo Tt o m oo — 'o S -° m O g 00 00 04 O4 m o r~- Os >n m O 8 o •n in o o "§S5 — — o — — • o — ~ ~~ — — ' — — 'S ° ' f) oo s oo s in o ON ON 04 s i S ON s r-- so — in r- " O* "^ ^— ^^ ^ 04 _ »J r*% p^ O _^ — — 1 1 i '« C "° S c 3 o r- »r> ro OO Q ^^ Os ^f O — O — aC^V>. lf | r~ 04 ^J 04 SO o QS [*• *** O O ON OJ X m TT m TT f*"l m ^ ^* f^t P « t) o > w w o o o 0 o o O O O O O 0 — 53 ~ 60 i i 1 1 1 1 • 1 1 1 1 ' 5-fl 1 m O4 Os oo 04 CO O4 O4 r- Os 04 oo O4 oo 0 O4 O m ON SO O — m 1 i-a O O o O O o o O o O O 0 E c g «« u ^> >j qj •*~ U_ u- U -^ UJ Os O oo m o o o m 0 oi o o o § ON S in o O4 OO O4 r*-, 0 O S-8 1 ™ o o o o o o o 0 O o 0 O -H +1 +1 +1 -hi -H -H -H -H -H -H -H w S •— **- _^ m ^J. Q Os f*"l m 04 Os Os r~ ^t >• "O ^a _M sO CO f^ _M ON OS OO ON so — JU | ^ ^J rrj f"J 04 r*"i ro O4 m O4 co rn ^* -C O O o O 0 O O O o O O O o 6o of ^^ P ':* ' § __ _- m m m m Tt so in f) ON o * O* g ON Tt O r~ r- oo OO (^ ON oo m 'S _c ^^ ^—i 04 ^J w Cy* u — y — So 5 6 Os O4 ro J. OO 4 Os 04 ^ •^ — rr •* 04 so 04 m 04 ^ C 60 <" o -r -o r~ ^3 -^ c Os oo OO ro so sO Q ON SO f*^ 04 l-li O4 O4 04 m 04 04 !§> § D. 2 £ J . j| ^^ J 4> C3 U •S i/) ^ (J JS 60 — co C " y 3 — •- OL \ ¥ .0 J u fl e § M U S ~ 1 2 fi 1 ^ 60_flJ "S | _w U U u 1 &o U o | 1 i I 'C "S. ° & 'd 3 Q X 'S Sj£ i/5 § g ^ 2 .s 1 1 I *» 'C •5 1 | .0 1 c 1 1 a 3 a Agelenopsis Callobius b £ •< Pardosa lap Oxyopes sal Pholcus pha Phidippus a Leucauge re Tetragnatha Achaearane Tmarus ang r5 — "> | e J2 1 II > I 1 Agelenidae. Amaurobiidae. Araneidae. Lycosidae. Oxyopidae. Pholcidae. Salticidae. Tetragnathidae. Tetragnathidae. Theridiidae. Thomisidae. Means 74 ENTOMOLOGICAL NEWS ACKNOWLEDGMENTS The assistance of Eric and Annabel Edwards in the field was greatly appreciated. The com- ments and suggestions of Jess Zimmermann, University of Puerto Rico, Los Piedras, and Stan Sady, Massachusetts General Hospital, Boston, who kindly critiqued the initial manuscript were most helpful. We thank the two anonymous reviewers for their comments and suggestions. Nancy McHugh, Northeast Fisheries Center, Woods Hole, kindly made laboratory facilities available. LITERATURE CITED Breymeyer, A. 1967. Correlations between dry weight of spiders and their length and fresh weight. Bull. acad. Pol. Sci. CI-II, XV(5):263-265. Clausen, I.H.S. 1983. Weight-length relations of eight species of spiders (Araneae) from Den- mark. Ent. Meddr 50: 139-144. Copenhagen, Denmark 1983. Edgar, W. D. 1971. Seasonal weight changes, age structure, natality and mortality in the wolf spider Pardosa lugubris in Central Scotland. Oikos 22:84-92. Edwards, R. L. 1993. Can the species richness of spiders be determined? Psyche. 100:(3-4)185- 208. Edwards, R. L. 1996. Estimating live spider weight using preserved specimens. J. Arachnol. 24:161-166. Greenstone, M. H., C. E. Morgan and A. Hultsch. 1985. Ballooning methodology: equations for estimating masses of sticky-trapped spiders. J. Arachnol. 13:225-230. Jocque, R. 1981 . Size and weight variations in spiders and their ecological significance. Biol. Jb. Dodonaea. 155-165. Vol. 109, No. 1 , January & February, 1998 75 OBSERVATIONS OF INTERACTIVE BEHAVIOR IN PARANDRA GLABRA (COLEOPTERA: CERAMBYCIDAE)1 Steven W. Lingafelter^ ABSTRACT: The first observed behavioral interactions for a species of Parandrinae (Coleoptera: Cerambycidae) are reported. Two sets of observations between males and females of Parandra glabra were made under artificial conditions: 1) individual male/female interactions; 2) interac- tions of multiple males in the presence of each female. Results of individual interactions indi- cate consistent patterns of behavior including antennation, mandibulation, genitalic exsertion, mounting, and dorsal-oblique mating posture. Males display aggression in first encounters with females, but subsequent antennation leads to mounting and copulation attempts. When more than one male is present with a female, these behavioral categories are directed toward other males more frequently than to the female. Intraspecific interactions in longhorn beetles can range from violent, indiscretionary encounters to complex, deliberate engagements, but the litera- ture contains few descriptions of these. Most observations are simply noted in more expansive papers concerning other biological or systematic issues. Michelsen (1966) focussed on interactive behavior when he provided the most detailed and widest coverage for behavior (especially with regard to courtship and copulation) in longhorned beetles. He provided data on species represent- ing four different subfamilies of longhorns (Aseminae, Spondylinae, Lepturinae, Cerambycinae). His behavioral observations were placed into 25 categories and a wealth of additional anecdotal information was included. Other refer- ences are restricted to fewer (usually one) species and often are much more general: Webster (1904) made interesting observations on Oberea ulmicola Chittenden (Lamiinae); Goldsmith (1987a, 1987b, 1989) examined mating systems of three species, Trachyderes mandibularis Dupont, Perarthrus linsleyi (Knull), and Stenaspis verticallis arizonicus Casey (all Cerambycinae); Hughes ( 1 98 1 ) examined mating behavior in Monochamus scutellatus (Say) (Lamiinae); Piper (1977) discussed mating behavior in Hippopsis lemniscata (Fabricius) (Lamiinae); Chemsak (1965) commented on habits of Oeme costata LeConte (Cerambycinae); Chemsak and Linsley (1971) observed mating behavior in Rosalia funebris Motschulsky (Cerambycinae); and Wang, et al. ( 1 990) looked at the complex mating behavior of Paraglenea fortunei Saunders (Lamiinae). This study on Parandra glabra (DeGeer) represents the first information on behavior in Parandrinae. 1 Received May 8, 1997. Accepted July 30, 1997. 2 Systematic Entomology Laboratory, PSI, ARS, USDAc/o U.S. Natural History Museum, NHB- 168, Washington, D.C. 20560. ENT. NEWS 109(1): 75-80 January & February, 1998 76 ENTOMOLOGICAL NEWS MATERIALS AND METHODS This study was undertaken at Monteverde Biological Reserve, Monteverde, Costa Rica. Three males and three females ofParandra glabra (DeGeer) were collected at lights, measured, and their date of collection noted. Measure- ments were taken of the total body length (from the base of the mandibles to the apex of the elytra; body width (distance between elytral humeri); length of mandibles (from the base to farthest point along medial axis) for correlation analyses. These data are listed in Table 1 . Table 1 . Data for specimens of Parandra glabra (DeGeer) used in this study. Specimen code (sex/#) Length Width Mandible length Date of Capture Ml 29mm 9.5mm 5.0 mm 22/May/1993 F2 31 mm 9.5 mm 3.0 mm 22/May/1993 M3 30 mm 10.0mm 5.0 mm 23/May/1993 F4 28mm 9.0mm 2.5mm 25/May/1993 F5 32mm 10.5 mm 3.0 mm 28/May/1993 M6 32mm 10.0mm 6.0 mm 30/May/!993 Specimens were marked on the base of the right elytron with an indelible black- ink marker. The beetles were simply coded with the number of the collection, "1-6". The beetles were kept separately in a small plastic container which was equipped with plastic dividers creating twelve separate holding cells. Damp wood was placed in each cell with the beetles. After several days of observing the beetles, their highest activity appeared to be at night, from 22:00 - 02:00. Formal observations were made during this period under low light conditions. Although specimens were strongly phototaxic to acute sources of light, low and diffuse lighting did not appear to alter their behavior. Two sets of investigations were made. The first involved nine pair comparisons of behavior between each male and female. The last focussed on interactive behavior when each female was placed with all three males. Each combination was observed for 20 minutes. RESULTS Observations: one on one male/female interactions Noted behaviors included antennation, mandibulation, genital ic exsertion, genitalic contact with substrate, mounting, and copulation. The number of incidents (or merely presence/absence) of these behavioral activities for each 20 minute observation period is indicated in Table 2. Vol. 109, No. 1, January & February, 1998 77 Antennation and mandibulation Males and females each opened their mandibles widely after initial contact. In most cases, the male relaxed and closed his mandibles after antennation of the female. In two instances, males bit the females upon initial contact but this was never repeated after further antennation. Genitalic exsertion and substrate contact In most cases, after prolonged antennation of the terminal abdominal segments of the female, the males exserted their genitalia partially or distended the region between the last two ventrites. This occurred an average of 2.9 times per male for each 20 minute observation period. In most cases the males would rub their genitalia on the substrate (the wood, container bottom, or container side) and in several cases on the female (the prothorax, mandibles, or elytra). It was distinctly obvious that muscular control of the genitalia, directing it downward, was being affected, and in most cases, the males would reverse walking direction several times during this behavior, rubbing the genitalia back and forth. Mounting Mounting of the female occurred an average of 2.2 times for each 20 minute observation period. In two pair combinations it did not occur. Both of these instances involved female #2, the only female not observed to copulate. Mounting usually occurred after contact with the female and antennation by the male, usually around her terminal abdominal segments. Usually in this scenario the females continued walking, somewhat oblivious to the male antennation. The male would then follow the female, maintaining contact and would mount her. In many cases, mounting would occur from the side as the female became "trapped" in a corner or against the piece of wood. This method was similar to that observed commonly in R.funebris (Cerambycinae) (Chemsak and Linsley, 1971). In some cases, after head-to-head contact between the pair, the male climbed on top of the female opposite her orientation, and in some cases, exserted his genitalia so it contacted her mandibles and prothorax. Often, a mounting involved probing by the male genitalia in search of the female's. The ratio of mountings to copulations was 4: 1 . Copulation Copulation is here defined as a visible, sustained contact between male and female genitalia. Transfer of sperm to the female could not be determined and thus was not a criterion for this category. Copulations were observed in four of the nine pair combinations. In one pair combination (male #1 ; female #5), copulation occurred twice during the twenty minute observation period. Copula- tions ranged in duration from 50 to 240 seconds, averaging 101 seconds. I interrupted a copulation in one instance as I was attempting to document it with a photograph. In every instance, despite preliminary mounting orientation, copulations occurred with the male atop the female in a slightly oblique position (ca. 30° from female central axis). The males extended their terminal abdominal 78 ENTOMOLOGICAL NEWS segments outward and probed with their genitalia for the female genital opening. The males did not forcibly extrude the female ovipositor as has been witnessed in other longhorn species (Michelsen, 1966). In many other longhorn species, Michelsen (1966) observed the male to actually leave the female's dorsum during copulation and in many cases face the opposite direction. During four of the five copulations, the female showed motility. In the one copulation where the female remained stationary (the second copulation of male #1 and female #5), pulsation of the male occurred about 45 times (about once every 5.5 seconds). This pulsation behavior was also noted in R.funebris by Chemsak and Linsley (1971). They witnessed this to last from 30 to 60 seconds and occur about once every four seconds. During this time the female remained motionless during the four-minute copulation. Interestingly, four of the five copulations involved female #5; female #2 had no copulations; and female #4 had only one. Observations: all males in presence of individual females For each of the three females, all three males together were placed with her and their actions were observed for about 20 minutes. Copulation was never observed in any of the three sets of observations; genitalic exsertion and rubbing on other males was observed in the presence of each female; same-sex mounting was observed in each combination; and a few instances of aggression between males was observed. Female #2 All three males contacted and mounted one another. Antennation of another male's sternites by each male was observed. Two instances of aggression were observed (male #6 biting male #1 ; male #6 biting male #3). Genitalic exsertion among the males was common during their juxtaposition, and they often rubbed their genitalia on one another. Mounting of the female occurred only once (by male #6); otherwise attention by males was directed toward other males. Female #4 The same behaviors as above were noted with the addition of the following: male #6 was seen rubbing his genitalia on the container. For a few minutes, all the males were in contact with the female and began mounting one another with their genitalia exserted. Female #5 Little attention was given to her as the males continued to mount and congregate with one another. DISCUSSION Because of the artificial conditions of this study and the small sample size of individuals, interpretation and generalization of the observations is made Vol. 109, No. 1, January & February, 1998 79 with caution. No correlations between size and exhibited behavior were evident from this study. Antennation seems to play an important role in the identification of females by males. Although observations of antennation were focussed on and biased toward males, it is evident that antennae are used to a greater extent by males than females. Obvious female antennation subsequent to a male encounter was rarely observed. Based on the rapidity in which males determined females by antennal contact (and then relaxed their mandibles and aggressive behavior), the antennae may have a great sensitivity to some chemical exudate of the female. Once the male established the presence of the female, subsequent contacts were the stimulus for his genital ic exsertion. In many of these instances, the male would rub his genitalia on the substrate and/or female. Based on observations of this behavior in both sets of experiments, it is possible that the male may be secreting some chemical, perhaps as a stimulus to cause the female to be receptive for copulation or as a chemical indicator of his presence to other males. Further study, both in natural conditions and with individual, laboratory reared specimens, is essential to understand the observations and interpretations presented herein. The phenomena of sperm precedence, male and female pheromones, body and mandible size correlations to mating success, and average number of mountings for each copulation or number of copulations required for successful sperm transfer, are interesting questions that may be addressed upon further study of the Parandrinae. Table 2. Summary of behaviors expressed by Parandra glabra (DeGeer) during the individual male/female pair combinations. A - antennation; MA - mandibulation; GE - genitalic exser- tion; GS - rubbing of genitalia on substrate; MO - mounting; C - copulation. For each category, numbers indicate number of observations of behavior, except for antennation which occurred numerous times in all pairs and is simply indicated with a checkmark. Duration of the five copulations is measured in seconds. Pair code (sex/# X sex/#) A MA GE GS MO C M1XF2 V 0 1 1 0 0 M1XF4 V 0 4 2 3 1 (50s) M1XF5 V 0 4 1 5 2 (65s & 240s) M3XF2 V i 3 1 2 0 M3XF4 V 0 2 0 1 0 M3XF5 V 0 5 3 4 1 (60s) M6XF2 V 1 2 1 0 0 M6XF4 V 0 1 0 1 0 M6XF5 V 1 4 1 4 1 (90s) 80 ENTOMOLOGICAL NEWS ACKNOWLEDGMENTS I thank the Department of Entomology (University of Kansas) for providing funds for field- work in Costa Rica. M. Greenfield provided logistical support for this work. I thank T. A. Erwin, D. G. Furth, R. S. Hanley, A. S. Konstantinov, A. L. Norrbom, and M. A. Solis for helpful sug- gestions on this manuscript. LITERATURE CITED Chemsak, J. A. 1965. A new subspecies of Oeme costata with observations on the habits of larvae and adults. Journ. Kans. Entomol. Soc. 38 (4):35 1-355. Chemsak, J. A. and E. G. Linsley . 1 97 1 . Some aspects of adult assembly and sexual behavior of Rosalia funebris Motschulsky under artificial conditions. Pan-Pac. Entomol. 47(2): 1 49- 1 54. Goldsmith, S. K. 1987a. The mating system and alternative reproductive behaviors ofDendrobias mandibularis (Coleoptera: Cerambycidae). Behav. Ecol. Sociobiol. 20: 1 1 1-1 15. Goldsmith, S. K. 1987b. Resource distribution and its effect on the mating system of a longhorned beetle, Perarthrus linsleyi (Coleoptera: Cerambycidae). Oecologia 73:317-320. Goldsmith, S. K. 1 989. Feeding ecology and the mating system of Stenaspis verticalis arizonicus Casey (Coleoptera: Cerambycidae). Journ. Kans. Entomol. Soc. 62(4): 528-533. Hughes, A. L. 1981. Differential male mating success in the white spotted sawyer Monochamus scutellatus (Coleoptera: Cerambycidae). Ann. Entomol. Soc. Am. 74: 180-184. Michelsen, A. 1966. The sexual behavior of longhorned beetles. Entomol. Medd. 34:329-355. Piper, G. L. 1977. Biology and habits of Hippopsis lemniscata (Coleoptera: Cerambycidae). Coleop's. Bull. 31(3):273-278. Wang, Q., Zeng, W. Y., and J. S. Li. 1 990. Reproductive behavior of Paraglenea fortunei (Coleoptera: Cerambycidae). Ann. Entomol. Soc. Am. 83(4): 860-866. Webster, F. M. 1904. Studies of the life history, habits, and taxonomic relations of a new species ofOberea (Oberea ulmicola Chittenden). Bull. Illinois State Lab. Nat. Hist. 7 (1): 1- 14+2 pis. When submitting papers, all authors are requested to (1) provide the names of two qualified individuals who have critically reviewed the manuscript before it is submitted and (2) suggest the names and addresses of two qualified authorities in the subject field to whom the manuscript may be referred by the editor for final review. All papers are submitted to recognized authorities for final review before acceptance. Titles should be carefully composed to reflect the true contents of the article, and be kept as brief as possible. Classification as to order and family should be included in the title, except where not pertinent. Following the title there should be a short informative abstract (not a descriptive abstract) of not over 150 words. 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MARCH & APRIL, 1998 NO. 2 NTOMOLOGICAL NEWS £ J\J ]" istruma memorialis (Hymenoptera: Formicidae), ^ new species of ant from Kentucky Cumberland Plateau Mark Deyrup 81 A new species of Aphaenogaster (Hymenoptera: Formi- cidae) from upland habitats in Florida M. Deyrup, L. Davis 88 Laboratory rearing of Mesovelia cryptophila (Heteroptera: Mesoveliidae) S.J. Taylor, J.E. McPherson 95 Two new species of microcaddisflies (Trichoptera: Hydrop- tilidae) from Kentucky R.E. Houp, K.H. Houp, S.C. Harris 99 Seasonal flight periodicities of six microcaddisflies (Trichoptera, Hydroptilidae, Glossosomatidae) in the Brazos River, Texas, with notes on larval biology D.C. Houghton, K.W. Stewart 103 A new species of Pseudocentroptiloides (Ephemeroptera: Baetidae), with revisions to previously unnamed baetid species from Texas NA. Wiersema, W.P. McCafferty 110 The adult of Moribaetis macaferti (Ephemeroptera: Baetidae) W. P. McCafferty, C.R. Lugo-Ortiz 117 First report and new species of genus Cloeodes (Ephemeroptera: Baetidae) from Australia C.R. Lugo-Ortiz, W.P. McCafferty 122 Diel emergence patterns of Tricorythodes stygiatus (Ephemeroptera: Leptohyphidae) on Little Lehigh Creek near Allentown, PA F. Gough, B.L. Haase 129 First fossil Dorylinae with notes on fossil Ecitoninae (Hymenoptera: Formicidae) Mark B. DuBois 136 Coccinellidae (Coleoptera) in apple orchards of eastern West Virginia, and impact of invasion by Harmonia axyridis M.W. Brown, S.S. Miller 143 SOCIETY MEETING OF OCTOBER 22, 1997 BOOKS RECEIVED AND BRIEFLY NOTED NOTE FROM EDITOR RE PRIORITY OF NEW DESCRIPTIONS 152 121,128 152 THE AMERICAN ENTOMOLOGICAL SOCIETY ENTOMOLOGICAL NEWS is published bi-monthly except July-August by The American Entomological Society at the Academy of Natural Sciences, 1900 Benjamin Franklin Park\vay, Philadelphia, Pa., 19103-1195, U.S.A. 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Boyd, 232 Oak Shade Road, Tabernacle Twp., Vincentown, New Jersey 08088, U.S.A. Manuscripts will be considered from any authors, but papers from members of the American Entomological Society are given priority. It is suggested that all prospective authors join the society. All manuscripts should follow the format recom- mended in Scientific Style and Format: The CBE Manual for Authors, Editors, and Publish- ers, Sixth Edition, and should follow the style used in recent issues of ENTOMOLOGICAL NEWS. Three doublespaced. typed copies of each manuscript are needed on 8'/2 x 1 1 paper. The receipt of all papers will be acknowledged and, if accepted, they will be published as soon as possible. Articles longer than eight printed pages may be published in two or more installments, unless the author is willing to pay the entire costs of a sufficient number of addi- tional pages in any one issue to enable such an article to appear without division. Editorial Policy: Manuscripts on taxonomy, systematics. morphology, physiology, ecology, behavior and similar aspects of insect life and related terrestrial arthropods are appropriate for submission to ENTOMOLOGICAL NEWS. Papers on applied, economic and regulatory entomology and on toxicology and related subjects will be considered only if they also make a major contribution in one of the aforementioned fields. (Continued on inside of back cover) Postmaster: // undelive rable , please send form 3579 to Howard P. Boyd, 232 Oak Shade Road, Tabernacle Twp., Vincentown, New Jersey 08088, U.SA. SECOND CLASS POSTAGE PAID AT VINCENTOWN, NEW JERSEY, 08088, U.S.A. Vol. 109, No. 2, March & April, 1998 81 SMITHISTRUMA MEMORIALIS (HYMENOPTERA: FORMICIDAE), A NEW SPECIES OF ANT FROM THE KENTUCKY CUMBERLAND PLATEAU1 Mark Deyrup^ ABSTRACT: A new species of dacetine ant, Smithistruma memorialis, is described. It was found on top of a ridge on the Cumberland Plateau in Kentucky. This species is distinguished from other North American species of Smithistruma by its short, erect, unmodified hairs on the oc- ciput, body and legs. It appears to be a member of the pulchella group of Smithistruma. It is named in honor of the late William L. Brown, Jr., who greatly advanced our knowledge of dacetine ants. The genus Smithistruma was described in 1948 by a pioneer of ant system- atics, the late William L. Brown, Jr., and his revision of the Nearctic species (1953) was so competent that it still serves well, almost 45 years after its pub- lication. The species described below is dedicated to Dr. Brown, in gratitude for his original work in bringing order to our knowledge of dacetine ants, and making the systematics and biology of these ants accessible to other natural- ists. This was only one of Dr. Brown's many contributions to myrmecology, biogeography, and evolutionary biology. Smithistruma includes about 104 described species (Bolton 1995). There are probably additional species to be discovered, as these ants are small (work- ers about 2 mm long), slow-moving, and usually hidden in leaf litter, rotten wood, or soil. Even in the eastern United States, where the cryptic ant fauna is relatively well known, there are a number of species that are rare in collec- tions, and it is still possible to find undescribed species. Smithistruma memorialis Deyrup, NEW SPECIES Diagosis. Distinguished from all other Nearctic Smithistruma by the presence of abundant, erect, unmodified hairs on the occiput, body and legs, and the lack of reclinate widened hairs in these areas (Fig. 1 ). The clypeus is similar to that of S. missouriensis (M. R. Smith) in shape and arrangement of enlarged hairs. Description. Holotype worker. Measurements in mm: Total length: 1 .98; head length: 0.5 1 ; maxi- mum head width: 0.40; length of alitrunk: 0.46. Features described below as in Fig. 1 . Head with preocular laminae not continuing the out- line of the occipital lobes, so outline of head in frontal view not cuneiform. Mandible in lateral view not tapering, but abruptly decurved at tip; mandibular diastema conspicuous at full closure; 1 Received June 25, 1997. Accepted August 1, 1997. 2 Archbold Biological Station, P. O, Box 2057, Lake Placid, FL 33862 ENT. NEWS 1 09(2) 8 1 -87, March & April, 1 998 82 ENTOMOLOGICAL NEWS mandibles with four principle teeth in the subapical series, the second largest, the first next larg- est, the fourth next largest, the third smallest. Clypeus with the apex rounded and thickened, central area of clypeus minutely reticulate, completely bare, slightly raised and diamond shaped; each side of clypeus with three small spoon-shaped hairs on the edge of the basal external corner, two large, spoon-shaped, anteriorly directed hairs on the anterior margin, at points about one- fifth and one half the distance to the apex, a large, spoon-shaped, posteriorly directed submedian hair, and two small, submarginal, submedian spoon-shaped hairs. Antennal scapes with enlarged, erect, untapered hairs on the inner margin as follows: one subbasal, directed slightly toward the apex of the scape, a series of four or five in descending size, more or less evenly spaced, directed slightly toward the base of the scape. Frontal area with small, sparse, inconspicuous, medially directed, reclinate spoon-shaped hairs. Occiput with sparse, erect, straight, blunt hairs, no espe- cially elongate or widened hairs on the lateral margins of the occiput. Upper part of head finely reticulate as in other members of the pulchella group, with inconspicuous, sparse, longitudinal rugae. Pronotum finely reticulate dorsally, sparsely and evenly covered with erect hairs, no elon- gate or otherwise distinctive hairs in the humeral area, smooth and shining laterally, convex in profile. Remainder of alitrunk with dorsum finely reticulate, with a few erect hairs, lateral areas smooth and shining. Propodeal teeth short, broad, infradental carinae narrow, evenly concave. Legs, including tarsi, with sparse, suberect hairs, without reclinate enlarged or spatulate hairs, no outstanding elongate curved or crimped hairs on the apical third of the mid or hind tibiae or basitarsi. Petiole finely reticulate dorsally, with sparse suberect hairs, infrapetiolar lamina narrow, without a basal lobe or extension, spongiform process small, in lateral view not extending to lower margin of petiole. Postpetiole with dorsal suberect hairs, lower spongiform process small, not extending down past upper half of basal face of first stemite of gaster. Caster with sparse, short erect hairs on dorsal surface, long dorsal hairs absent. Figure 1 . Smithistruma memorialis, new species Vol. 109, No. 2, March & April, 1998 83 Paratype female. Measurements in mm: Total length: 2.45, head length: 0.68, maximum head width: 0.47, length of alitrunk: 0.57. Usual queen modifications present: ocelli present, compound eyes large, alitrunk modified for flight. Otherwise, queen resembles worker, including abundant short, erect hairs on dorsum of thorax, gaster and legs. This vestiture distinguishes this specimen from queens of other dacetine species. Paratypes. Paratype material is 61 workers and one queen. Collecting data for type material. All type material shares the same data: KENTUCKY: Laurel Co., Daniel Boone National Forest, Bald Rock picnic area; 23 March 1997; collected by Stephen and Mark Deyrup. Habitat open, grassy, with low herbs, scattered large pines. Nest with holotype worker, allotype queen, and 53 workers found (by Stephen) in a small chamber in clay soil a few centimeters below the surface near the base of a large pine, near the restrooms; 8 workers from a small soil sample about three meters away. Other ants with nearby nests were: Aphaenogaster tennesseensis (Mayr) (dealate queen), Brachymyrmex depilis Emery, Leptothorax pergandei Emery, Mornomorium minimum (Buckley), Paratrechinafaisonensis Forel, Ponera pennsylvanica Buckley, Prenolepis imparts (Say), Smithistruma pulchella (Emery) (dealate queen), Solenopsis carolinensis (Forel). Deposition of type material. Holotype, allotype, 12 paratypes: Museum of Comparative Zool- ogy, Harvard University, Cambridge, Mass.; 6 paratypes: National Museum of Natural History, Smithsonian Institution, Washington, D.C.; 6 paratypes; The Natural History Museum, London; 6 paratypes: Los Angeles County Museum of Natural History; 6 paratypes: Florida State Collec- tion of Arthropods, Gainesville, Fla.; 3 paratypes, collection of Lloyd Davis, Gainesville, Fla.; 3 paratypes: Collection of Mark DuBois, Washington, III.; 3 paratypes, collection of William MacKay, El Paso, Tex.; remaining specimens in the collection of the Archbold Biological Sta- tion, Lake Placid, Fla. Etymology. The specific epithet, translated "of remembrance," dedicates this species to Bill Brown in place of the more usual patronym. For some years Bill Brown had held an antipathy toward patronyms. His main objection, as far as I can tell, was that patronyms often honor people who have only the most trivial association with the species bearing their name, and in the worst cases could be assigned to stoke the egos of sponsors or patrons. I do not think these objections would apply in the case of an honoree who had made great and lasting contributions to our knowledge of a genus, especially when the name is applied posthumously. Nevertheless, I am respecting his feelings by avoiding a direct patronym. DISCUSSION Myrmecologists who deal with dacetines in general and with Smithistruma in particular place a well-justified faith in the taxonomic value of the elabora- tions of the clypeus and mandibles as species-specific character states. In the absence of any plausible theories explaining the remarkable diversity of these features, it is easy to develop an illogical feeling that their biological function is also associated with species recognition, like the modified palps and facial 84 ENTOMOLOGICAL NEWS hairs that distinguish certain male dolichopodid flies in their courtship antics. In S. memorialis we see a species that might never have been recognized on the basis of its clypeal structure, which is similar to that of the variable species S. missouriensis (Fig. 3). This is a useful, if somewhat worrisome reminder that in Smithistruma the shape of the clypeus and the pattern of its pilosity could remain constant in a group of related species. Smithistruma memorialis clearly belongs in Brown's pulchella group, along with missouriensis, reflexa, and cloydi. Smithistruma memorialis (Fig. 1), reflexa (Fig. 2), and missouriensis have mandibles that in lateral view are broad and abruptly decurved in their apical third, while the mandibles of pulchella (Fig. 4) and cloydi (Fig. 5) are slender and tapering. Smithistruma pulchella Figure 2. Smithistruma reflexa (Wesson and Wesson) Figure 3. Smithistruma missouriensis (Smith) Vol. 109, No. 2, March & April, 1998 85 Figure 4. Smithistruma pulchella (Emery) Figure 5. Smithistruma cloydi Pfitzer 86 ENTOMOLOGICAL NEWS and cloydi differ markedly in the shape of the clypeus and in the number and arrangement of clypeal hairs (Figs. 4, 5). Smithistruma reflexa and missouriensis are distinguished by differences in the orientation of clypeal hairs (Figs. 2, 3), a character that is somewhat variable, and reflexa could be a junior synonym of missouriensis (Brown 1953); the small series of missouriensis that I have studied appears virtually identical to reflexa in lateral view. In Brown's 1953 key, memorialis keys to missouriensis (second half of couplet 23), but is easily distinguished by its bristling hairs. Now that it is clear that species recognition in this complex may depend on more than clypeal hairs, other structural char- acters should be examined more closely, and we should be cautious in synony- mizing species. The described native species of Smithistruma in the southeastern U. S. now number 24, and there are at least two additional undescribed species (Deyrup and Cover, MS in preparation), bringing the known species from the region to 26. Southeastern North America is therefore a major center of diver- sity for the genus, and Brown (1953) suggested that this fauna is most closely related to that of Asia, rather than to the Neotropical fauna, as had been sug- gested earlier. Ward (1988) described three species from relict warm mesic areas in the southwestern U.S. This pattern closely matches the distribution of relict concentrations of warm temperate arcto-tertiary flora (Raven and Axelrod 1978). The genus Smithistruma, therefore, appears to be our only clear ex- ample among the ants of a diverse assemblage left over from the gloriously speciose warm temperate forests of the Miocene. Most of the known species of North American Smithistruma are quite widely distributed through the mixed deciduous forests of the Middle Atlantic states, south through north Florida, and west into the more mesic woodlands of eastern Texas. If, however, the genus Smithistruma in North America mirrors the distribution of arcto-tertiary flora, there are probably some species confined to isolated habitat types in the southern Appalachians. The species described here, found on top of a ridge on the Cumberland Plateau, could represent such a species. It seems appropriate to place memorialis in Brown's genus Smithistruma, even though there are some indications that this genus may disappear in a small implosion of dacetine genera. Unfortunately, Bill Brown will not be around to offer his comments. In a general way, he felt that some changes were necessary: in a recent (2 February, 1997) letter he stated, "...there is no doubt that generic slaughter is overdue." However, he also wrote, in the same letter, "...I hate to see all the names go down, and I'll be watching." As most contem- porary myrmecologists know, Bill Brown thought that defining a genus on a strictly phyletic basis could lead to a foolish nomenclature. Paraphyletiphobia was not one of his afflictions. In his address at the 1987 meeting of the Ento- mological Society of America, he publicly expressed the opinion that super- specific names, such as the names of genera, are inevitably derived by an exer- Vol. 109, No. 2, March & April, 1998 87 cise of judgement, and these judgements should be openly informed by ecol- ogy and convenience, as well as by phylogenetics. He strongly objected to examples of supposedly objective phyletic nomenclature that were really de- rived from the secret manipulation of an arcane analysis. I wish I could re- member all his much more humorous private comments at this convention. There was one particularly funny comparision of some cladistic taxonomy to astrology, in which all the character states and accomplishments of life are subsidiary to, and mystically influenced by, the exact moment of separation from the mother. Within the narrow confines of a paper describing one species of dacetine ant, I have tried to touch on the extreme importance of Bill Brown to our understanding of the systematics and biogeography of this group. I would not want to leave the impression that the study of dacetines will falter; for some years the tremendously talented and energetic Barry Bolton of The Natural History Museum, London, has been carrying on the work of large-scale revi- sions of dacetines. What we have lost is the presence of the man, fantastically knowledgeable, honest, critical, humorous, creative and intuitive, who could inspire both nervousness and relief when he said, "I'll be watching." ACKNOWLEDGMENTS I am proud to acknowledge our son Stephen as the collector of the large nest series of Smithistruma memorialis I also thank the custodians of the Daniel Boone National Forest for watching over the beautiful and diverse habitats of the area, where I am sure that many more undescribed species of interesting insects will be found. LITERATURE CITED Bolton, B. 1995. A new general catalog of the ants of the World. Harvard Univ. Press, Cam- bridge, Mass. 504 pp. Brown, W. L., Jr. 1948. A preliminary generic revision of the higher dacetini. Trans. Amer. Entomol. Soc. 74: 101-129. Brown, W. L., Jr. 1953. Revisionary studies in the ant tribe Dacetini. Amer. Midi. Natur. 50: 1 - 137. Raven, P. H., and D. I. Axelrod. 1978. Origin and relationships of the California flora. Univ. Cal. Publ. Bot. 72: 1-134. Ward, P. S. 1988. Mesic elements in the western Nearctic ant fauna: taxonomic and biological notes on Amblyopone, Proceratium, and Smithistruma ). Kans. Entomol. Soc. 61: 102-124. 88 ENTOMOLOGICAL NEWS A NEW SPECIES OF APHAENOGASTER (HYMENOPTERA: FORMICIDAE) FROM UPLAND HABITATS IN FLORIDA Mark Deyrup^, Lloyd Davis^ ABSTRACT: Aphaenogaster umphreyi, n. sp., is described from sandy uplands of peninsular Florida. It appears to be closely related to A.fulva Roger. The new species, which may be entirely subterranean in habits, is characterized by unusually small eyes, coarse sculpture, short propodeal spines, and unusually small hind tibial spurs. The genus Aphaenogaster tends to accumulate taxonomic problems. A number of species show conspicuous variation between populations, within populations, and even within colonies, while camouflaged in this tangle of intraspecific variation are cryptic species that can only be detected by the most sophisticated methodology, such as that used by Umphrey (1996) for the A. rudis group. One species that emerged some time ago from the taxonomic thicket \sA.fulva Roger, which, once divested of the set of "varieties" that are now recognized as the rudis group, seemed to be a single, easily recognized species (Creighton, 1950). It now appears that there is a second species that shares most of the features previously ascribed solely to fulva. Fortunately, since this species is rare, or at least difficult to find, it is unlikely to have been the basis of many (if any) published records of fulva. Aphaenogaster umphreyi, Deyrup and Davis, NEW SPECIES Figure 1 Description: Holotype worker measurements (mm): head length (anterior edge of clypeus to occiput) 1.15; head width (above eyes): 0.95; malar space ( = distance from lower edge of eye to mandible in lateral view): 0.38; length of eye: 0.15; distance from propodeal spiracle to tip of propodeal spine: 0.31. In frontal view, head with convex vertex; coarse reticulate rugae covering dorsum and sides of head, including occiput; venter of head with prominent carinae diverging from midline. Man- dible, antenna, and clypeal area resembling those of fulva (cf. Figs. 1 and 2). Mesosoma with strongly raised rugae on the pronotum, mesonotum, and propodeum, these rugae zigzagging, not smoothly undulating, except less elevated and more undulating on pronotal disc. Propodeal spine short, compared to that of fulva (Fig. 2), strongly upturned, approaching a right angle with long axis of propodeum. Legs generally similar to fulva, including transverse ridges on front coxae, except hind and middle tibial spurs reduced, shorter than width of basitarsus on respective legs. 1 Received June 6, 1997. Accepted August 30, 1997. 2 Mark Deyrup, Archbold Biological Station, P. O. Box 2057, Lake Placid, FL 33862. 3 Lloyd Davis, 3920 NW 36th Place, Gainesville, FL 32606. ENT. NEWS 109(2) 88-94, March & April, 1998 Vol. 109, No. 2, March & April, 1998 89 Figure 1 . Aphaenogaster umphreyi, new species, worker. 1mm Figure 2. Aphaenogaster fulva, worker, Florida specimen. 90 ENTOMOLOGICAL NEWS Petiole and gaster similar lofulva (cf. Figs. 1 and 2), except petiole with more conspicuous rugae. Color reddish brown, legs and gaster yellowish brown. Diagnosis. Similar to A.fulva, but differs in having much smaller eyes, shorter propodeal spines, coarser and more extensive sculpture on the head and mesosoma, more convex vertex in frontal view, and reduced hind tibial spurs (compare Figs. 1 and 2). Type material. Holotype worker: FLORIDA, Putnam Co., 3 miles east of Melrose, 20 Aug. 1995 (Lloyd R. Davis), Ordway Preserve, sandhill habitat, nest in ground, at base of small oak; deposited in Museum of Comparative Zoology, Harvard University, Cambridge, Massachusetts. Paratypes: FLORIDA: 22 workers from nest series of holotype; same site and collector as holotype: 1 worker: 1 Oct. 1995; 1 worker: 24 Feb. 1995; 1 worker: 27 Aug. 1995; 1 worker: 6 Sept. 1996; 2 workers: 14 Sept. 1996; 2 workers: 3 Mar. 1995. One worker: Highlands Co., Archbold Biological Station, 16 May 1988 (M. Deyrup), sifted from sand, Florida scrub habitat; 1 worker: Highlands Co. Sebring, 11 Mar. 1987 (M. Deyrup), Red Water Lake, Florida scrub habitat; 2 workers: Highlands Co., Sebring 17 Sep. 1990 (M. Deyrup), Flamingo Villas develop- ment, Florida scrub habitat; 10 workers (callows, nest series): Marion Co., 16 Oct. 1990 (M. Deyrup), Ocala Waterway development, Florida scrub habitat; 3 workers: Alachua Co., 5.5 miles west of Gainesville (L. Davis), in soil beside rotten pine log, open oak woodland, 22 Mar. 1992; 6 workers: Highlands Co., Placid Lakes Development (M. Deyrup), 1 Jan. 1997, Florida scrub habitat, collector's yard, root mat below Quercus inopina. Deposition of paratypes: 4: Museum of Comparative Zoology, Harvard University; 5: Na- tional Museum of Natural History, Smithsonian Institution, Washington, D.C.; 5: Florida State Collection of Arthropods, Gainesville; 5: The Natural History Museum, London; 4: Los Angeles County Museum of Natural History; 3: collection of Gary Umphrey, London, Ontario; 2 paratypes: collection of Mark Dubois, Washington, Illinois; 2: collection of Kye Hedlin, Raleigh, North Carolina; 2: collection of William MacKay, El Paso, Texas; 5: collection of Lloyd Davis, Gainesville, Florida; 17: Arthropod Collection, Archbold Biological Station. Etymology. This species is named in honor of Dr. Gary Umphrey, in recognition of his long labors working to elucidate the taxonomy and phylogeny of the intractable A. rudis group. DISCUSSION Although we have known of specimens of an aberrant Aphaenogaster for almost nine years, we were wary about assigning them to a new species be- cause of the notorious intraspecific variation within the genus, and the result- ing history of synonymy. Our hypothesis was that there might be a southern isolate offulva that differed in various ways from northern forms, and it was not until we had a good series of the new species from within the range of fulva in north Florida (Fig. 3) that this hypothesis became untenable. We had also hoped to find many more colonies, and associated sexuals, though these aims still elude us. Meanwhile, we gathered specimens offulva from much of its range, so that variation within that species is now clearer to us. Vol. 109, No. 2, March & April, 1998 91 The evidence that we use to establish the species-level distinctness of umphreyi is as follows: 1 . A. umphreyi is sympatric with/w/va, at least in Putnam and Marion Counties, and probably farther north as well, so umphreyi is not likely to be a geographic subspecies of fulva. 2. There is no overlap in the following structural character states used to distinguish the new species: rela- tively small eyes (Fig. 4); relatively small propodeal spines; heavy zigzag cari- Aphaenogaster fulva Aphaenogasler umphreyi Figure 3. Collection sites for Aphaenogaster umphreyi and A. fulva in Florida. 92 ENTOMOLOGICAL NEWS nae on the sides of the pronotum and on the propodeum; reduced spurs on the middle and hind tibiae. 3. The occurrence ofumphreyi in xeric habitats, while fulva (at least in the southeast) is in mesic, often wet sites. 4. The kinds of morphological differences between the two species go far beyond the kinds of intraspecific variation that seem to be directly influenced by environmental conditions in different habitats (e.g.: in xeric habitats Pheidole dentata Mayr seems paler, Odontomachus brunneus (Patton) paler and smaller). The evidence available suggests that umphreyi and fulva are a closely re- lated species pair. They share exclusively the following character states: coarse sculpture on the head and mesosoma; upward-pointing propodeal spines; strongly elevated, notched anterior edge of the mesonotum. This combination of features brings umphreyi out to couplet 18 in Creighton's key (1950) to Aphaenogaster, but the short propodeal spines produce an impasse. Color is not a very reliable character in fulva; it is generally dark brown, but we have seen reddish specimens, particularly from its western range. It 26 XvIylvXvXvXvXvXvXvXvIvXvXvXv & OJ CD C 0> .25 .24 23 .22 .21 .20 .19 .18 .17 16 15 14 .13 .12 .11 19 f3 f2 f12 f7 f8 '5 'vX:X; te .:::x:x: no 111 U7 us :•:•:•:•:•: u8 u6 u 1 2 u11u9 u 1 u3 u10 u4 .27 28 29 .30 31 .32 .33 .34 35 .36 37 .38 39 40 .41 Malar Space (=distance from lower margin of eye to mandible) Figure 4. Malar space and eye length in Aphaenogaster umphreyi (u), representing 10 colonies, and A. fulva (f), representing 12 Florida colonies. Vol. 1 09, No. 2, March & April, 1 998 93 would still be useful to check reddish brown individuals assigned to fulva in collections to see whether they have smaller eyes and the other characteristics of umphreyi. We have provisionally identified as umphreyi a pair of pale speci- mens with small eyes collected in Decatur County, Georgia, but have not in- cluded them among the paratypes because they are small specimens with much less conspicuous rugae than normal in umphreyi', these specimens are in the Museum of Comparative Zoology, Harvard University. The reduced hind and middle tibial spurs of umphreyi are quite variable. In some specimens they are absent, in others they are present, but short. The hind tibial spurs, when present, are shorter than the middle tibial spurs. The hind tibial spurs in Aphaenogaster as a whole are remarkably expressive, compared with most ant genera. In A.floridana Smith they are broad and abruptly acumi- nate. In A. pallida (Nylander), which also has very small eyes, they are ex- tremely reduced. In A.flemingi Smith, the hind tibial spurs are somewhat re- duced, shorter than the middle tibial spurs. In A. tennesseensis (Mayr), they are much reduced, thick, and sometimes curved. In A. sardoa Mayr, and A. senilis Mayr, the basal third is noticeably swollen. In A. campana Emery, they are bristle-like. In A. cockerelli Andre, they have apparently been lost, and replaced by enlarged lateral bristles. Aphaenogaster fulva and several other species have evenly tapering spurs. Nobody knows, of course, what ecological and evolutionary factors are affecting tibial spur morphology, but a study of the habits of umphreyi and other species that have unusual spurs might help us understand the function of tibial spurs in ants. Aphaenogaster umphreyi may be almost entirely subterranean in habits, and if it does emerge, it may do so at night. All the specimens were collected underground, either in sand under a thick layer of dead leaves and roots, or under piles of litter and trash. The senior author has spent hundreds of hours over the last 14 years prowling scrub habitat at the Archbold Biological Sta- tion, without seeing a single specimen of umphreyi in the open. The small eyes and pale color of umphreyi are consistent with a subterranean life. There are no sexuals associated with workers of umphreyi, but we believe that we may have collected an unassociated queen. Carroll (1975) states that queens of fulva are easily distinguished by the "heavily rugose mesothoracic episternite and sternite." The worker-associated queens we have seen from Florida, South Carolina, Maryland, and Arkansas seem to agree with this de- scription, the rugosity consisting of long, gently undulating longitudinal rugae on a granulate background. We have one dealate queen from Archbold Bio- logical Station (where fulva is unknown) that has zigzag rugae on the me- sothoracic episternite and reduced spurs on the hind and middle tibiae. The eyes and propodeal spines are not reduced. The specimen was collected in a window trap in Florida scrub habitat in November, 1987. Nothing is known about umphreyi other than it is a subterranean inhabitant 94 ENTOMOLOGICAL NEWS of sandy uplands of the southeast, it is difficult to collect, and it appears to be closely related tofulva. We have no long series showing intraspecific varia- tion, no associated sexuals, no details of its geographic range, and no informa- tion on diet or behavior. Until myrmecologists develop an effective method for finding colonies, this will remain one of our most obscure species of Aphaenogaster. ACKNOWLEDGMENTS We thank Gary Umphrey (University of Western Ontario) and Stefan Cover (Harvard Uni- versity) for reviewing drafts of this paper. We thank Stefan Cover for arranging the loan of speci- mens from the Museum of Comparative Zoology, Harvard University, and Walter Suter, Zachary Prusak, and David Corey for contributing specimens of Aphaenogaste rfulva, and Fabrizio Rigato for contributing specimens of several species of European Aphaenogaster. We thank our wives, Nancy and Marie, for waiting patiently whenever we left them by the roadside and went off into the woods for "a few minutes" of ant collecting. LITERATURE CITED Carroll, J. F. 1 975. Biology and ecology of ants of the genus Aphaenogaster in Florida. Unpub- lished Ph.D. Thesis, Univ. Florida, Gainesville. 177 pp. Creighton, W. S. 1950. The ants of North America. Bull. Mus. Comp. Zool. Harvard Univ. 104: 585 pp. Umphrey, G., J. 1996. Morphometric discrimination among sibling species in the fulva-ru dis- texana complex of the ant genus Aphaenogaster (Hymenoptera: Formicidae). Can. J. Zool. 74: 528-559. Vol. 109, No. 2, March & April, 1998 95 LABORATORY REARING OF MESOVELIA CRYPTOPHILA (HETEROPTERA: MESOVELIIDAE)1 Steven J. Taylor^, J. E. McPherson^ ABSTRACT: Mesovelia cryptophila was reared from egg to adult at 26.7±0.6° C under a 1 4L: 1 OD photoperiod. The incubation period averaged 14.93 days; and the four nymphal stadia, 3.24, 2.51, 3.15, and 4.85 days, respectively. Mesovelia cryptophila Hungerford occurs from New Jersey south to Florida and west to Michigan, Iowa, Oklahoma, and Mississippi (Smith 1988); it also has been reported from Minnesota (Bennett and Cook 1981) and Texas (Polhemus 1997). Little is known about the biology of this infrequently collected species. It has been collected from a shaded pool in Mississippi (Wilson 1958); a cypress swamp in South Carolina (Sanderson 1982); bog, lake, and impoundment habi- tats in New Jersey (Chapman 1959); a small pond and shaded, stagnant back- waters of a stream in Iowa (Harris 1943); and the margin of a bog lake in Michigan, similar to the habitat of Mesovelia amoena Uhler (reported as Mesovelia douglasensis Hungerford) (Hoffmann 1932, Hungerford 1924). Hoffmann (1932) reported only apterous adults in Michigan, and Sanderson (1982) stated that macropterous adults were unknown. Chapman (1959) re- ported specimens (stages not given) collected in May and August through Oc- tober in New Jersey; Sanderson (1982) collected specimens (stages not given) in July in South Carolina; and Hungerford (1924) and Hoffmann (1932) col- lected adults in June and July, and in July, respectively, in Michigan. Hoffmann (1932) reared this species in the laboratory under unspecified conditions with limited success (i.e., five individuals reached adult) and briefly described the immature stages. He reported only four nymphal instars. On 9 July 1991, one of us (SJT) discovered a small population of M. cryptophila in Gallatin County, Illinois. Because so little is known about this insect, and because Hoffmann's (1932) rearing data were limited and collected under unspecified conditions, we decided to rear the insect in our laboratory under controlled conditions. 1 Received June 23, 1997. Accepted August 23, 1997. 2 Center for Biodiversity, Illinois Natural History Survey, 607 E. Peabody Dr., Champaign, IL 61820. 3 Department of Zoology, Southern Illinois University at Carbondale, Carbondale, IL 62901- 6501 USA. ENT. NEWS 109(2) 95-98, March & April. 1998 96 ENTOMOLOGICAL NEWS MATERIALS AND METHODS The Gallatin County site was a small, shaded bay of Crab Lake ( = Hulda Lake) located 6 mi NE of Shawneetown. The water was covered with a light (approximately 10 plants per m^) covering of duckweed (Lemanaceae). Thirty apterous adults (15C?C?,1599) were collected with an aquatic D-net up to 2 m from shore, brought to the laboratory, and placed in glass creamers (1C?, 1 9 /container). The creamers (4.5 cm deep x 3.0 cm diam.) were filled with 1 cm of deionized water and 3 floating plastic disks (0.6 cm in diam.) were added. The curved sides of the containers were sufficient to prevent the insects from escaping. Two paper strips of cardstock (approximately 1.25 x 2.5 cm) were angled against opposite sides of each container with the tops above the water. The disks and strips served as oviposition sites and allowed individuals to leave the water. Containers were checked daily for eggs, all of which were laid beneath the water surface. Plastic disks and paper strips, with attached eggs, were trans- ferred to new containers. If eggs were deposited on the walls of the container, adults were transferred to a new container. As eggs hatched, the newly emerged first instars were transferred to new containers prepared similarly to those for adults, but without paper strips. Nymphs of the same instar molting into the subsequent instar on the same day were transferred to new containers if other nymphs in the container had not molted. The water level was maintained just above (0.1 - 0.3 cm) the eggs. Maximum numbers of individuals reared per container were: 6 first instars, 4 second instars, 3 third instars, and 1 fourth instar. Adults reared from these eggs were preserved in 70% ethanol. Each adult was fed 1, and each nymph 1/2, frozen adult fruit fly (Droso- phila melanogaster Meigen) per day. Flies were crushed or torn slightly for nymphs to facilitate feeding and were replaced daily. All individuals were maintained in incubators at 26.7 ± 0.6° C under a 1 4L: 10D photoperiod. All containers were changed at least once per week but more frequently when water became cloudy. Data were analyzed with the SAS (SAS Institute 1988) TTEST procedure. Level of significance was set at 0.05. RESULTS AND DISCUSSION Eggs (n = 96) were deposited singly on the paper strips, sides of the plastic disks, and walls of the containers. The incubation period averaged 14.93 days (Table 1). There were four nymphal instars, thus confirming Hoffmann's (1932) results. The first through fourth stadia averaged 3.24, 2.51, 3.15, and 4.85 days, respectively. Duration of total developmental time from egg to adult averaged 28.56 days. No sexual difference was detected for either the fourth Vol. 109, No. 2, March & April, 1998 97 stadium (T = -0.9685, df = 45, p = 0.3304) or for total length of development (T = -0.8387, df = 45, p = 0.4061). Of the 30 field-collected adults, five fe- males and four males were still alive after one month. Hoffmann (1932) collected adults in Michigan but reared their offspring in Kansas. Females laid up to 75 eggs (mean = 55, n = 4), which were inserted into plant tissue. He reported an egg laid on 27 July hatched on 1 4 March (229 days), and two eggs laid on 24 and 25 July hatched between 21 and 25 Septem- ber (58-63 days). The large discrepency in incubation period between Hoffmann's study (58-229 days) and ours (12-19 days) (Table 1) suggests his eggs were in diapause. Hoffmann's (1932) five specimens reared from first instar to adult averaged 1 7.6 days (range = 1 6-21 , no rearing temperature given), approximately four days longer than ours. Although the occurrence of only four instars is rare in Gerromorpha (Stys and Davidova-Vilimova 1989), it is not unprecedented in the Mesoveliidae; Mesoveliafurcata Mulsant and Rey, a European species, has only four nymphal instars (Zimmerman 1984). Table 1 . Durations (in days) of immature stages of laboratory-reared Mesovelia cryptophila. Number completing Stage Sex stadium Mean ± Std. Err. Range Egga 94 14.93 + 0.16 12-19 First instar 80 3.24 ±0.07 2-5 Second instar 67 2.51 ±0.07 2-4 Third instar 71 3. 15 ±0.07 2-4 Fourth instar Males + Females'3 48 4.85 ±0.11 3-7 Males 27 4.74 ±0.1 5 3-7 Females 20 4.95+0.15 3-6 Egg through fourth instar Males + Females^ 48 28.56 + 0.20 25-31 Males 27 28.41 ±0.26 25-31 Females 20 28.75 + 0.32 26-31 a 96 eggs were laid. " One individual died during molting and could not be sexed. 98 ENTOMOLOGICAL NEWS ACKNOWLEDGMENTS We thank the following individuals for their critical reviews of the manuscript: R. A. Bran- don, J. A. Beatty, B. M. Burr, Department of Zoology; and D. Ugent, Department of Plant Biol- ogy, Southern Illinois University at Carbondale. LITERATURE CITED Bennett, D. V. and E. F. Cook. 1981. The semiaquatic Hemiptera of Minnesota (Hemiptera: Heteroptera). Minn. Agric. Exp. Stn. Tech. Bull. 332:1-59. Chapman, H. C. 1959. Distributional and ecological records for some aquatic and semi-aquatic Heteroptera of New Jersey. Bull. Brooklyn Entomol. Soc. 54:8-12. Harris, H. M. 1943. A note on the range of Mesovelia cryptophila Hungerford (Hemiptera: Mesoveliidae). J. Kansas Entomol. Soc. 16:53-54. Hoffmann, C. H. 1932. The biology of three North American species of Mesovelia (Hemiptera- Mesoveliidae). Can. Entomol. 64:88-95, 113-120, 126-134. Hungerford, H. B. 1924. A second new Mesovelia from the Douglas Lake, Michigan region (Hemiptera-Mesoveliidae). Ann. Entomol. Soc. Am. 17:453-456. Polhemus, J. T. 1997. New state and U. S. records and other distributional notes for Heteroptera (Insecta). Entomol. News 108:305-310. Sanderson, M. W. 1982. Aquatic and semiaquatic Hemiptera, pp. 6.1-6.94. In: A. R. Brigham, W. U. Brigham, and A. Gnilka (eds.). Aquatic insects and oligochaetes of North and South Carolina. Midwest Aquatic Enterprises, Mahomet, Illinois. 837 pp. SAS Institute. 1988. SAS/STAT user's guide, version 6, 4th ed. Vol. 2. SAS Institute, Gary, North Carolina. 479 pp. Smith, C. L. 1988. Family Mesoveliidae Douglas and Scott, 1867, pp. 247-248. In: T. J. Henry and R. C. Froeschner (eds.). Catalog of the Heteroptera, or true bugs, of Canada and the continental United States. E. J. Brill, New York. 958 pp. Stys, P. and J. Davidova-Vilimova. 1989. Unusual numbers of instars in Heteroptera: a review. Acta Entomol. Bohemoslovaca 86: 1-32. Wilson, C. A. 1 958. Aquatic and semiaquatic Hemiptera of Mississippi. Tulane Stud. Zool. 6: 1 16- 170. Zimmermann, M. 1984. Population structure, life cycle and habitat of the pondweed bug Mesovelia furcata (Hemiptera, Mesoveliidae). Revue Suisse Zool. 91: 1017-1035. Vol. 109, No. 2, March & April, 1998 99 TWO NEW SPECIES OF MICROCADDISFLIES (TRICHOPTERA: HYDROPTILIDAE) FROM KENTUCKY1 Ronald E. Houp2, Katherine H. Houp3, Steven C. Harris4 ABSTRACT: Two new species of microcaddisflies, Hydroptila howelli and Hydroptila kuehnei (Trichoptera: Hydroptilidae), from Kentucky are described and their affinities noted. The caddisfly fauna of the southeastern United States has been fairly well studied, but new species continue to be found. This paper describes two new species in the genus Hydroptila from a single locality along Salt Lick Creek in Kentucky, an unaltered Reference Reach stream, draining a portion of the "Knobs", an area within the Interior Plateau ecoregion. Terminology used in the descriptions follows that of Marshall (1979). Specimen length was mea- sured from the tip of the head to the end of the wings and is given as a range. Type material will be deposited at the National Museum of Natural History, Smithsonian Institution (NMNH), the Illinois Natural History Survey (INHS), the Branley A. Branson Museum of Zoology, Eastern Kentucky University (BABMZ) and the collections of the authors. Hydroptila howelli NEW SPECIES (Fig. 1) Description. Male. Length 1.9 - 2.1 mm. 27 antennal segments. Brown in alcohol. Venter of abdominal segment VII with short apicomesal process. Segment VIII triangular in lateral view, acute posteroventrally; in ventral view, deep rounded incision posteromesally, laterally terminat- ing in several sclerotized teeth; nearly rectangular in dorsal aspect. Segment IX retracted within segment VIII in ventral view; in dorsal view, anterior portion retracted within VIII and mesally incised, posterior portion divided into pair of truncate lateral lobes, widely separated mesally. Tenth tergum broadly triangular, laterally with elongate, sinuate sclerotized processes with acute apices turned inward; in lateral view these thin processes sharply turned ventrad. Subgenital plate a rounded shelf in ventral view, bearing pair of short setae posteromesally. Inferior append- ages in lateral view thin and elongate, clublike at apex; in ventral view widely separated, nearly parallel along mesal margin, outer margins sinuate, apices acute and strongly turned outward. Phallus tubular, widening at base and narrowing at midlength, ejaculatory duct protruding apically about 1/4 phallus length, thin paramere at midlength encircling shaft. Female and larva. Unknown. Type material. Holotype, male. Kentucky, LaRue-Marion County line, Salt Lick Creek on Salt Lick Road, 17 May 1996, at blacklight, R. Houp and K. Houp (NMNH). Paratype, same locality asholotype, 1 male (BABMZ). 1 Received May 21, 1997, Accepted August 20, 1997. 2 Kentucky Division of Water, 14 Reilly Road, Frankfort, KY 40601 . 3 Department of Biology, Midway College, Midway, KY 40347. 4 Department of Biology, Clarion University, Clarion, PA 16214. ENT. NEWS 109(2) 99-102, March & April, 1998 100 ENTOMOLOGICAL NEWS Etymology. Named for the late Dr. Henry H. Howell, in honor of his con- tributions to aquatic ecology. A teacher, friend and mentor. Diagnosis. This species is similar to several species in the H. waubesiana group, including H. delineata Morton, H. tridentata Holzenthal and Kelly, H. englishi Hamilton, and H. grandiosa Ross. From the latter two species, H. howelli is separated by the lack of elongate, heavy spines from the sternum of abdominal segment IX. The new species is separated from other species in the waubesiana group by the ventral elongation of segment VIII, the elongate, sinuate lateral straps of the tenth tergite, and the thin, widely separated inferior appendages which are strongly hooked apically. Hydroptila kuehnei NEW SPECIES (Fig. 2) Description. Male. Length 2.8 - 3.1 mm. 28 antennal segments. Brown in alcohol. Venter of abdominal segment VII with short apicomesal process. Segment VIII annular; slightly incised along posterior margin in ventral view; dorsally with pair of crescent-shaped sclerites posteriorly. Segment IX elongate posterodorsally in ventral view; in dorsal view, emarginate posteriorly, elon- gate processes laterally, deeply incised anteriorly. Segment X fused with IX, dorsum divided at base into pair of thin arms, nearly parallel sided, distally with acute points apically and subapi- cally; in lateral view, arms separated distally with apical points dorsad and ventrad. Subgenital plate in ventral view thin, lateral margins curved inward, rounded apically bearing pair of mesa! setae. Inferior appendages elongate and thin in lateral view, sharply curved downward at base, apically with sclerotized ventral point; in ventral view short and slightly elbowed outward, heavy peglike setae subapically, elongate seta on lateral margin subapically and basally. Phallus tubular, widening at base, ejaculatory duct protruding distally, elongate paramere encircling shaft near midlength. Type material. Holotype, male. Kentucky, LaRue-Marion County line, Salt Lick Creek on Salt Creek Road, 7 August 1996, at blacklight, R. Houp and K. Houp (NMNH). Paratypes, same data as holotype, 5 males (NMNH, INHS, BABMZ, REH, SCH). Etymology. Named for the late Dr. Robert A. Kuehne, and his contributions to aquatic ecology. A friend, teacher and mentor. Diagnosis. This species, another member of the H. waubesiana group, is most similar to H. patriciae Harris and H. eramosa Harper. With these two species, H. kuehnei shares the lateral elongation of abdominal segment IX and the division of the tenth tergite into two lateral arms. These lateral arms are elongate in the new species, but short in both H. patriciae and H. eramosa and terminate in long apical extensions in H. patriciae, but short acute extensions in H. kuehnei and H. eramosa. The inferior appendages are strongly elbowed in H. patriciae and to the lesser degree in H. kuehnei, but they are nearly straight in H. eramosa. As well, these appendages in both H. eramosa and the new species have a strong peglike seta apically which is absent in H. patriciae. The subgenital plate, in ventral view, in both H. patriciae and H. kuehnei is thin apically, but Vol. 109, No. 2, March & April, 1998 101 broadly rounded in H. eramosa. This combination of characters serves to distinguish the new species from other members of the H. waubesiana group. A • ^S-4-T -' jy^H \ .'.i I )•- xCs/M ,\ i^ /\':/'$ I ' ^K^K ' - ' /»*^ B Figure 1. Hydroptila howelli, n. sp. male genitalia. A. Lateral view; B. Dorsal view; C. Ventral view; D. Phallus, ventral view. 102 ENTOMOLOGICAL NEWS D Figure 2. Hydroptila kuehnei, n. sp. male genitalia. A. Lateral view; B. Dorsal view; C. Ventral view; D. Phallus, ventral view. ACKNOWLEDGMENTS The authors appreciate the interest and assistance of G. A. Schuster, Eastern Kentucky Uni- versity. LITERATURE CITED Marshall, J. E. 1979. A review of the genera of the Hydroptilidae (Trichoptera). Bull. Brit. Mus. (Nat. Hist.), Entomol. Ser. 39: 135-239. Vol. 109, No. 2, March & April, 1998 103 SEASONAL FLIGHT PERIODICITIES OF SIX MICROCADDISFLIES (TRICHOPTERA: HYDROPTILIDAE, GLOSSOSOMATIDAE) IN THE BRAZOS RIVER, TEXAS, WITH NOTES ON LARVAL BIOLOGY AND SITE RECORDS1 David C. Houghton2'3, Kenneth W. Stewart2 ABSTRACT: The seasonal flight periodicities of six small caddisflies were studied from April, 1995, to November, 1996, at a large riffle of the Brazos River in north-central Texas. Light trap samples for adults suggested a synchronized univoltine cycle for Protoptila alexanderi, asyn- chronous univoltine cycles for Hydroptila icona and Orthotrichia cristata, bivoltine cycles for Ithytrichia clavata and Ochrotrichia tarsalis, and a multivoltine cycle for Hydroptila angusta; the last species accounted for 82% of the total hydroptilid adult abundance. Larval collections of P. alexanderi, H. angusta and /. clavata confirmed their presence in the benthic community. Seven site records and one drainage record of Neotrichia vibrans were documented, increasing the total number of caddisfly species found at this site by Moulton et al. (1993) to 30. The caddisfly family Hydroptilidae, often referred to as the "micro- caddisflies", contains the smallest members of the Trichoptera, with adults of many of the 220 Nearctic species reaching only 2-3 mm in length (Wiggins 1996). Hydroptilidae is the most species rich trichopteran family, and often ten or more congeners can occur together at a single site (Moulton and Stewart 1996). Despite this, very little is known concerning hydroptilid life histories and behavior. Nielson (1948) studied the biology of five Danish species; eluci- dating univoltine cycles for Orthotrichia tetensii Kolbe and Ithytrichia lamellaris Eaton, and bivoltine cycles for Agraylea multipunctata Curtis, Hydroptila femoralis Eaton and Oxeythira costalis Curtis. This work remains the source for much of the knowledge of larval morphology, feeding, case- building behavior and general biology of the family (Marshall 1979, Wiggins 1996). Other ecological studies of hydroptilids have dealt with the drift patterns of larvae, including those of Hydroptila rono Ross and H. icona Mosely, re- spectively, in an Oregon and Texas stream (Anderson 1967, Cloud and Stewart 1974a) from which an interpretation of univoltine life cycles was made by Wiggins (1996). Resh and Houp (1986) studied the biology of Dibusa angata Ross and found it to be bivoltine in a Kentucky stream and dependent on the red alga Lemanea australis Atkinson for food and case-building material. Marshall (1979) reviewed the world genera of Hydroptilidae. 1 Received May 15, 1997. Accepted July 25, 1997. 2 Department of Biological Sciences, University of North Texas, P.O. Box 5218, Denton, TX 76203-0218. 3 Current address: Department of Entomology, University of Minnesota, St. Paul, MN 55108. ENT. NEWS 109(2) 103-109, March & April, 1998 104 ENTOMOLOGICAL NEWS Moulton et al. (1993) documented 23 species of Trichoptera from a riffle of the Brazos River in Palo Pinto County, Texas as part of their investigation of the Brazos River caddisfly fauna. This documentation included 10 hydroptilid species and one member of the glossosomatid caddisfly subfamily Protoptilinae, Culoptila cantha (Ross). Protoptiline glossosomatids are often field-sorted in with hydroptilids due to their small size; no life history information has been reported for any of the 18 North American species (Wiggins 1996). The pur- pose of our study was to document the seasonal flight periodicities of micro- caddisflies at this Brazos River site as suggestive of their respective voltinisms. MATERIALS AND METHODS Study site. The Brazos River arises on the Caprock Escarpment in eastern New Mexico and flows southeasterly through Texas to the Gulf of Mexico. Our study was conducted at a 200 m riffle located approximately 35 km down- stream of the Morris Shepard Dam in Palo Pinto County; research on the biol- ogy of many aquatic insects has been ongoing at this site for 25 years (Stewart et al. 1973; Cloud and Stewart 1974a,b; Vaught and Stewart 1974; McClure and Stewart 1976; Rhame and Stewart 1976; Houghton 1997). Recent descrip- tions of this site can be found in Moulton et al. (1993) and Houghton (1997). Adults. Adults were collected from April, 1995, to November, 1996, using two 8-watt portable ultra-violet lights placed over two 22.5 X 35 cm white porcelain pans filled with 70% ETOH. These samples were generally taken monthly from October to March and twice monthly from April to September. The pans were set about 1.0 m from the water's edge; one at the head of the riffle, and the other 16.5 m downstream. Preliminary observations had indi- cated predominately nocturnal flight periods for Brazos microcaddisflies; there- fore lights were run for 20 minute intervals beginning at dusk, separated by 40 minutes between samples, until three sets of samples were taken or until a sample yielded no adults of any species. A small number ofHydroptila angusta Ross adults were aspirated from riparian rocks and vegetation prior to dusk during February and March, 1996. Each sample taken was sub-sampled by the following procedure: the specimens were placed in a 9.5 cm diameter round petrie dish and mixed thoroughly; a 3 cm diameter ring was randomly placed into the sample and all individuals within the ring area were counted; the num- ber of males and females of each species found was extrapolated to estimate the total number present in each sample; the samples were combined to esti- mate the number present on each sampling date. Larvae and Pupae. Larvae and pupae were collected off rocks with soft- touch forceps on the same sampling dates as adults and either preserved in 70% ETOH or transported alive back to the laboratory in Styrofoam "six-pack" coolers (Szczytko and Stewart 1979). Larvae and pupae were reared in wire Vol. 109, No. 2, March & April, 1998 105 mesh baskets within a Frigid Units Living Stream™ with Brazos River simu- lated flow, temperature, photoperiod, and natural algal food to estimate pupa- tion period and associate the life stages using the metamorphotype method (Milne 1938). Behavior of fifth instar larvae was observed by placing indi- viduals in a 5 cm diameter petrie dish under a Wild M2E dissecting micro- scope equipped with a Dyonics fiberoptic light source. Voucher specimens of all species studied are deposited in the University of North Texas Entomologi- cal Collection. RESULTS AND DISCUSSION Seasonal flight periodicities were determined for the hydroptilid species Hydroptila icona, H. angusta, Ithytrichia clavata Eaton, Ochrotrichia tarsalis (Hagen) and Orthotrichia cristata Morton, and for Protoptila alexanderi Ross (Glossosomatidae: Protoptilinae) (Fig. 1); the life history of the protoptiline glossosomatid Culoptila cantha, which was very common at this site, was stud- ied by Houghton (1997) and will be published in a separate paper. Protoptila alexanderi and Ochrotrichia tarsalis represented site records not reported by Moulton et al. (1993), as did the following species which did not occur in enough abundance to discern a flight periodicity pattern: Hydroptila waubesiana Betten (n = 63), Neotrichia minutisimella (Chambers) (n = 1 ), N. vibrans Ross (n = 2), Oxeythira aculea Ross (n = 3), and O. azteca (Mosely) (n = 2). Neotrichia vibrans also represented a new drainage record. Protoptila alexanderi Ross. Adults of this species were caught from early July to late September, 1995, and early August to October, 1996 (Fig. 1 ). Peak abundances occurred in late August, 1995, and early September, 1996, sug- gesting a synchronized univoltine cycle. Males were slightly more abundant than females; both sexes exhbited similar seasonal periodicity. We collected a series of twelve fifth instar larvae in early July, 1995, on clean 10-20 cm diam- eter stones in the upstream half of the riffle. Moulton et al. (1993) collected adults from the Paluxy River and Ham Creek, Brazos River tributaries, in June and October. Previously, the species had been reported in Texas only from the San Antonio and San Marcos Rivers (Edwards 1973). Hydroptila angusta Ross. Adults of this species were collected in all months of the year (Fig. 1 ) and accounted for 82% of the total hydroptilid sample. As many as four generations may have occurred during both years as suggested by the peaks in abundance, although an exact determination is not possible without extensive larval data. Males and females exhibited similar seasonal periodicity. Larvae were reared to adults during April, July, and September, 1 995, and March, April, and June, 1 996. Moulton et al. ( 1 993) collected adults throughout their Brazos Drainage study area during the months of March- April, 106 ENTOMOLOGICAL NEWS May -June, and October. Cloud and Stewart ( 1 974a) did not report this species in the drift of the Brazos River. We found substantial numbers of fifth instar larvae in the filamentous algal growth (mostly Cladophora sp.) that was com- mon on most of the rocks in the riffle during summer months. When placed on small stones in a petrie dish, larvae were sedentary and held their cases at acute angles to the faces of the stones. Hydroptila icona Mosely. Adults of this species were collected from early May to November during both years (Fig. 1). Peak abundances of both sexes occurred in late June, 1995, and early July, 1996, suggesting a univoltine cycle; this would corroborate the one-year cycle proposed by Cloud and Stewart (1974a) and Wiggins (1996). However, voltinism is confounded by a second peak in male abundance that occurred in late August, 1995, and early August, 1996. Moulton et al. (1993) collected adults throughout their study area during the months of June and October-November. Cloud and Stewart (1974a) re- ported H. icona as one of the two common hydroptilids at this site and noted a nocturnal drift of both cased and caseless larvae. We did not find larvae of this species in our benthic samples. Ithytrichia clavata Eaton. Adults of this species were collected from early April to November, 1995, and early April to October, 1996 (Fig. 1). Adult abundance peaked during early May and early September, 1995, and late April and late September, 1996, suggesting a synchronized bivoltine cycle. Males were slightly more abundant during the early generation and females were slightly more abundant in the later generation during both years. A series of 1 2 pupae collected in early April, 1995, emerged in the laboratory in early May, 1995; a series of 6 pupae collected in early April, 1996, emerged in late April, 1996. We also collected approximately 50 empty cases in early April, 1996, and only one cased larva in early May, 1995. These pupae and empty cases were found on rocks in quiet areas of the riffle. Moulton et al. (1993) collected adults throughout most of their study area, including this site, during the months of March- April, June and October. Despite the low numbers of larvae and pu- pae that we found, Cloud and Stewart (1974a) reported that /. clavata was a common hydroptilid at this site and that it drifted nocturnally. The one fifth instar larva was found on a rock near the head of the riffle. When placed on a small stone in a petrie dish under microscopic light and videotaped, this indi- vidual exhibited strong negative phototaxicity and actively sought the under- side of the stone. A torsion angle of 90° between the first two abdominal seg- ments, causing the flat sides of the case to be held parallel to the ground, was evident. This phenomenon was also noted for/, lamellaris (Nielson 1948). Ochrotrichia tarsalis (Hagen). Adults of this species were collected from early June to November, 1995, and early April to October, 1996. Adult abun- Vol. 109, No. 2, March & April, 1998 107 200 100 0 10,000 ,C 5,000 U o £ 2,000 ^2 1 ,000 1 ° J> 800 B 3 400 | 0 ~CS 400 ."&? 200 0 600 300 -*- Males — Females Protoptila alexanderi Hydroptila angusta Hydroptila icona Ithytrichia clavata Ochrotrichia tarsalis Orthotrichia cristata AMJ J ASONDJ FMAMJ J ASON 1995 1996 Month Figure 1 . Estimated number of adults caught from a riffle on the Brazos River, Texas from April, 1995, to November, 1996, based on subsamples. 108 ENTOMOLOGICAL NEWS dance peaked in late July and late September, 1995, and early July and Octo- ber, 1996 (Fig. 1), suggesting a synchronized bivoltine cycle. Males and fe- males had similar seasonal periodicity. Moulton et al. (1993) did not collect this species at our site but found adults in much of their Brazos Drainage study area during June and October. Cloud and Stewart (1974a) reported Ochrotrichia larvae in low abundance in their drift samples but did not identify a species. We did not find larvae of this species in our study riffle. Orthotrichia cristata Morton. Adults of this species were collected from late June to November, 1995, and early June to November, 1996 (Fig. 1). Both sexes peaked in abundance in late September during both years, suggesting a univoltine cycle. However, females were not collected until early August both years, while males were present for 3-4 months before their peak abundance. Moulton et al. (1993) collected adults at our site during June and July. Cloud and Stewart (1974a) report Orthotrichia larvae in low abundance in their drift samples but did not identify a species. We did not find larvae of this species in our sampling area. ACKNOWLEDGMENTS The authors thank S.R. Moulton, II for confirming the identity of several species and the following people for assisting us in the field and laboratory: J.C. Abbott, I.W. Agado, G.L. Akin, R.G. Jones, C.J. Larson, J.C. Norwood, S.A. Peterson, J.P. Snow and N.L. Witt. We also thank K.D. Alexander, S.W. Szczytko and two anonymous reviewers for commenting on earlier ver- sions of our manuscript. LITERATURE CITED Anderson, N. H. 1967. Biology and downstream drift of some Oregon Trichoptera. Can. Entomol. 99: 507-521. Cloud, T. J. and K. W. Stewart. 1974a. Seasonal fluctuations and periodicity in the drift of caddisfly larvae (Trichoptera) in the Brazos River, Texas. Ann. Entomol. Soc. Am. 67: 805- 810. Cloud, T. J. and K. W. Stewart. 1974b. The drift of mayflies (Ephmeroptera) in the Brazos River, Texas. J. Kans. Entomol. Soc. 47: 379-396. Edwards, S. W. 1973 Texas caddisflies. Texas J. Sci. 23: 491-516. Houghton, D. C. 1 997. Descriptions, life history and case-building behavior of Culoptila cantha (Ross) (Trichoptera: Glossosomatidae) in the Brazos River, Texas. M.S. thesis. Univ. North Texas, Denton, TX. 62 pp. Marshall, J. E. 1979. A re view of the genera of Hydroptilidae (Trichoptera). Bull. British. Mus. Natur. Hist., Entomol. Sen 39: 135-239. McClure, R. G. and K. W. Stewart. 1976. Life cycle and production of Chorterpes (Neo- chorterpes) mexicanus Allen (Ephemeroptera: Leptophlebiidae). Ann. Entomol. Soc. Am. 69: 134-148. Milne, M. J. 1938. The "metamorphotype" method in Trichoptera. J. N.Y. Entomol. Soc. 46: 435-437. Moulton II, S. R. and K. W. Stewart. 1996. Caddisflies (Insecta: Trichoptera) of the Interior Highlands of North America. Mem. Am. Entomol. Inst. 58: 1-313. Vol. 109, No. 2, March & April, 1998 109 Moulton II, S. R., Petr, D., and K. W. Stewart. 1993. Caddisflies (Insecta: Trichoptera) of the Brazos river drainage in north-central Texas. Southw. Natur. 38: 19-23. Nielson, A. 1 948. Postembryonic development and biology of the Hydroptilidae. Kongelige Danske Videnskabernes Selskab, Biolgiske Skrifter 5( 1 ). 203 pp. Resh, V. H. and R. E. Houp. 1986. Life history of the caddisfly Dibusa angata and its associa- tion with the red alga Lemanea australis. J. N. Am. Benthol. Soc. 5: 28-40. Rhame, R. E. and K. W. Stewart. 1976. Life cycles of three Hydropsychidae (Trichoptera) species in the Brazos River, Texas. Trans. Am. Entomol. Soc. 102: 65-99. Szczytko, S. W. and K. W. Stewart. 1979. Stonefly drumming as a model classroom study of aquatic insect behavior. In: Resh, V. H. and D. M. Rosenberg (eds.) Innovative teaching in aquatic entomology. Can Spec. Publ. Fish. Aquat. Sci. 43: 31-37. Stewart, K. W., G. P. Friday and R. E. Rhame. 1973. Food habits of hellgrammite larvae, Corydalus cornutus (Megaloptera: Corydalidae), in the Brazos River, Texas. Ann. Entomol Soc. Am. 66: 959-963. Vaught, G. E. and K. W. Stewart. 1974. The life history and ecology of Neoperla clymene (Newman) (Plecoptera: Perlidae). Ann. Entomol. Soc. Am. 67: 167-178. Wiggins, G. B. 1996. Larvae of the North American caddisfly genera (Trichoptera). Univ. Toronto Press. Toronto, Canada. 457 pp. 1 10 ENTOMOLOGICAL NEWS A NEW SPECIES OF PSEUDOCENTROPT1LOIDES (EPHEMEROPTERA: BAETIDAE), WITH REVISIONS TO OTHER PREVIOUSLY UNNAMED BAETID SPECIES FROM TEXAS1'2 N. A. Wiersema3, W. P. McCafferty4 ABSTRACT: Pseudocentropriloides morihari, new species, is described from larvae and male and female adults from Texas, USA. The species represents the second of the genus to be discov- ered in North America, where it is distinguished from P. usa primarily by size, labral and maxil- lary morphology, and ventral abdominal pattern in the larvae. Pseudocentroptiloides morihari was previously reported as Centroptilum sp. 2. Some other unnamed but numbered species of Baetidae reported from Texas are resolved to known nominal species ofAcerpenna, Baetis, and Centroptilum, and the status of others is reviewed. Difficulty in separating adult males of Pseudocentroptiloides and some Procloeon is noted. The small minnow mayfly genus Pseudocentroptiloides Jacob (Ephe- meroptera: Baetidae) was revised by Waltz and McCafferty (1989) and has included P. ceylonica Glazaczow (Oriental), P. christineae Waltz and McCaf- ferty (Oriental), P. shadini (Kazlauskas) (Palearctic; type species), and P. usa Waltz and McCafferty (Nearctic). Larvae are characterized by a V-shaped emar- gination on the labrum, broad maxillae, labial palp segments 3 that are greatly expanded and truncated, and glossae that are relatively short and truncate. In 1977, one of us (WPM) collected very small and then unidentifiable larvae in Texas that were simply referred to as Centroptilum sp. 2, and subse- quently reported as such by McCafferty and Davis (1992) and Lugo-Ortiz and McCafferty (1995).Recently,the other of us (NAW) collected additional larvae of this species in Texas and was able to rear adults. Close examination of the material in light of recent revisions of North American Baetidae (see e.g., McCafferty and Waltz 1990, Edmunds and Waltz 1996) shows that it is clearly Pseudocentroptiloides and that it represents a previously undescribed species. Prior to this discovery, P. usa, from the Midwest, was the only species of Pseudo- centroptiloides known from the Western Hemisphere, and it and P. shadini were the only species known as adults. McCafferty and Waltz (1990) had predicted that additional species of Pseudocentroptiloides eventually would be found in North America. We are most honored to name this new species after Dennis Morihara (Pukalani, Hawaii), who helped collect the original material, and who contributed significant systematic work that catalyzed our modern understand- ing of the diverse and challenging family Baetidae in North America. 1 Received August 18, 1997. Accepted September 10, 1997. 2 Purdue Agricultural Experiment Station Journal No. 15478. 3 4857 Briarbend, Houston, TX 77035. 4Dept. Entomology, Purdue University, West Lafayette, IN 47907. ENT. NEWS 109(2) 110-116, March & April, 1998 Vol. 109, No. 2, March & April, 1998 Because of the state of knowledge at the time, a number of baetids col- lected from Texas could not be assuredly assigned to nominal species and thus were reported as numbered species by McCafferty and Davis ( 1 992) and Lugo- Ortiz and McCafferty (1995). New analyses of much of that material, often in light of subsequent collections, have allowed us to update the identification status of those species. We provide this new information under the heading Additional Species Revisions following the new species description below. Pseudocentroptiloides morihari, NEW SPECIES (Figs. 1-8) Centroptilum sp. 2 McCafferty and Davis, 1992:207. Larva. Lengths: body 4.0-5.5 mm; caudal filaments 1.6-2.0 mm. Head: Coloration pale, darkest around coronal and frontal sutures. Antennae broken but extending at least as far as fore coxae; scapes, pedicels, and flagella with sparse, fine, simple setae. Labrum (Fig. 1) sclerotized posterolaterally, slightly wider than long, and with broad triangular emargination apically; dorsal subapical setae present across entire width of labrum, relatively short and not extending beyond, or much beyond, distal margin of labrum. Mandibles (Figs. 2 and 3) with relatively broad bases. Maxillae (Fig. 4) with crest of fine setae extending basally well below galealacinia crown, setae longer or subequal to galealacinia denticles; digitate setae of galealacinia comb poorly devel- oped (1-2 setae); palpi three segmented. Labium as in Fig. 5, with glossae nearly as wide as long and two-thirds to three-fourths length of paraglossae. Thorax: Nota cream, without distinct pat- terns. Hindwingpads present. Legs similar to Figs. 6 and 7 of Waltz and McCafferty (1989); claws subequal in length to respective tarsi. Abdomen: Coloration pale; terga light brown, dark- est at anterior margins of each tergum. Sterna unicolorous cream, except for wide transverse brown band anteriorly on sternum 8 [mature individuals also with purplish transverse markings of adults (see below) on sterna 7 and 8]. Paraprocts with five to six marginal spines. Median caudal filament subequal in length to cerci. Male adult. Lengths: body 4.6-5.8 mm; forewings 3.8-5.0 mm; hindwings 0.8-1.0 mm; cerci 8.0-10.0 mm. Head: Turbinate portion of compound eyes yellow, slightly divergent anteri- orly, oval, ca. 1.6 times longer than wide in dorsal view, and on low stalks. Thorax: Coloration generally cream to light brown, sterna slightly paler than nota. Forewings (Fig. 6) with marginal intercalaries relatively long, especially posterior to MA2 (longer than respective distal crossveins connecting longitudinal veins between MA2 and ICuA)). Hindwings (Fig. 7) slightly broad- ened, with short marginal veins following 2nd main longitudinal vein. Abdomen: Segments 2-6 translucent white; segments 7-10 slightly darker; small longitudinal dashes laterally on segments 1-9; prominent black, complex, internal maculation visible in dorsal view on segments 7 and 8. Sterna 7 and 8 often with transverse, pale reddish purple line anteriorly. Genitalia as in Fig. 8. Female adult. Lengths: body 5.6-5.8 mm; forewings 5.0 mm; hindwings 1 .0 mm; cerci 6.5- 7.0 mm. Body more robust than male, generally pale throughout except for prominent black spiracular markings dorsolaterally on abdomen, black line in cervical area, and faint dark mark- ings laterally on thorax. Material examined. HOLOTYPE: male larva, TEXAS, Austin Co., San Bernard River at I- 10, west of Sealy, V-9-1977, W. P. McCafferty, A. V. Provonsha, D. Morihara, in the Purdue Entomological Research Collection. PARATYPES: one male and one female larva, same data and deposition as holotype. Additional material: One female larva and one reared male adult (some parts mounted on slides), TEXAS, Austin Co., San Bernard River at I- 10 west of Sealy, V- 1 9- 1 997, N. A. Wiersema, same deposition as holotype. Three male and two female adults, TEXAS, Williamson Co., Georgetown, San Gabriel River at San Gabriel Park, X-7- 1 996, N. A. Wiersema, 1 1 2 ENTOMOLOGICAL NEWS same deposition of holotype, except one male in private collection of NAW. One male adult, TEXAS, Comal Co., Guadalupe River about 1 1 miles below Canyon Dam, XII-7-1996, N. A. Wiersema, in private collection of NAW. Diagnosis. Larvae of P. usa are much larger and more robust than those of P. morihari, with mature individuals nearly twice as large. Structurally, P. morihari can be further easily distinguished from P. usa by the shape of the distal emargination of the labrum (Fig. 1 ), dorsal labral subapical setae that extend in a row along the entire width of the labrum (Fig. 1), the much more extensive crest of setae on the galealaciniae (Fig. 4), and relatively broader glossae and labial palps segment 3 (Fig. 5) [see comparative figures off. usa in Waltz and McCafferty (1989): Figs. 1,4, and 5)]. In addition, whereas the paraprocts of P. morihari have five or six marginal spines, re-examination of the type material of P. usa indicated that they have six or seven. This latter difference is of inconsistent value for diagnosis; however, significant color pattern differences were also revealed from the type material of P. usa. Whereas P. morihari larvae have a somewhat broad band of pigment anteriorly on ab- dominal sternum 8, in P. usa such a band is prominent on both sterna 8 and 9 and weakly present on sternum 7. In addition, sublateral paired spots that are present on all the abdominal sterna of P. usa, but particularly well developed on sterna 7-9, are entirely absent in P. morihari. In the larval key to world species of Pseudocentroptiloides provided by Waltz and McCafferty (1989), P. morihari can most easily be incorporated into it simply by adding another couplet at the end. First, however, the couplet part 3' should be modified to read: "Maxillae [Figs. 3 1 (Keffermuller and Sowa 1984) and 4 herein] with crest of setae extending basally below crown of galealacinia, subequal to or longer than denticles of galealacinia . . . 4." Cou- plet 4 should then be added as follows: 4. Labrum (Fig. 1 herein) with relatively short dorsal subapical setae not extending beyond, or much beyond, distal margin of labrum P. morihari. 4'. Labrum [Fig. la of Jacob and Glazaczow (1986)] with long dorsal subapical setae extending well beyond distal margin of labrum P. shadini. The adults of P. morihari and P. usa are remarkably similar. Structurally, the only apparently significant differences found involve the wings. In P. morihari, the marginal intercalaries of the forewing are relatively long, espe- cially in the posterior half of the wing. Whereas the intercalaries are longer than the respective crossveins connecting longitudinal veins between MA2 and ICuA[ in P. morihari (Fig. 6), they are shorter by the same comparison in P. usa [Fig. 8 of Waltz and McCafferty (1989)]. Also, the hindwings of P. morihari (Fig. 7) are slightly broader and have an additional short vein posteriorly. The hindwings of P. usa are very narrow and have only two long longitudinal veins [Fig. 9 of Waltz and McCafferty (1989)]. The abdominal segments 1-6 of the male adults are light in color in both species, as is common in many baetid Vol. 109, No. 2, March & April, 1998 13 Figs. 1-8. Pseudocentroptiloides morihari, n. sp., 1-5. Larva. 1. Labrum (right: dorsal, left: ven- tral). 2. Left mandible. 3. Right mandible. 4. Left maxilla. 5. Labium (half, dorsal view). 6-8. Male adult. 6. Forewing. 7. Hindwing (enlarged relative to forewing). 8. Genitalia (ventral). 1 1 4 ENTOMOLOGICAL NEWS males, but the thorax and abdominal segments 7-10 are slightly more darkly contrasting in P. usa than in P . morihari. One other possible difference be- tween the males of these species is that in all the material known thus far, the anterior line on abdominal sterna 7 and 8 is broken in the middle in P. usa, thus appearing as a pair of lines rather than a single line as in P. morihari. Unfortu- nately, we cannot be sure if any of the adult differences we have reviewed above will be consistent between the species when larger populations are known. All such characteristics have been known to be variable in other adults of cer- tain baetids. Although the female adult of P. usa was not described by Waltz and McCafferty (1989), our examination of the female adult paratype of P. usa indicated that the females of P. morihari and P. usa are very similar, except for the venational differences noted above. In addition, based on the few compara- tive specimens available, the branching spiracular maculations appear more developed in P. usa, and there is a long, horizontal, unbroken, thin, dark line connecting abdominal segments 8 and 9 laterally in P. usa that is not present as such in P. morihari. Remarks. The San Bernard River where larvae of P. morihari have been collected has a shifting sand substrate. Larvae were kicked into a screen or dredged with a D-net from sand and vegetation in relatively shallow water. Adults have been collected at lights at night (as subimagos) from the Guadalupe and San Gabriel Rivers. All of the localities are in the Hill Country of central Texas. Lugo-Ortiz and McCafferty (1995) showed that this area had the high- est mayfly diversity in Texas. They also indicated that this area was strongly influenced by eastern North American faunal elements. Caution should be taken when sorting Texas adults with single marginal intercalaries in the forewings, including P. morihari. We have recently reared Procloeon viridoculare (Berner) from Texas that as adults have genitalia with a medial protrusion on the forceps that will make them key out to Pseudo- centroptiloides when using the generic key by Edmunds and Waltz (1996). Because there is no doubt that they are P. viridoculare, and because we have seen the slight development of such a protrusion on other Procloeon in the South and Midwest, there is a potential problem in separating Procloeon from Pseudocentroptiloides when only adults are in hand. ADDITIONAL SPECIES REVISIONS Acentrella sp. 1 Lugo-Ortiz and McCafferty [= Baetis virilis (McDun- nough)]. The original report of Acentrella sp. 1 by Lugo-Ortiz and McCafferty (1995) was based on the male larval stage of Baetis virilis. This species lacks hindwings, and because of leg setation was provisionally placed in Acentrella at the time. This also represents the first report of B. virilis from Texas. Acentrella sp. 2 Lugo-Ortiz and McCafferty [= Baetis virilis (McDun- Vol. 109, No. 2, March & April, 1998 115 nough)]. The fact that the female larvae originally referred to a&Acentrella sp. 2 by Lugo-Ortiz and McCafferty (1995) also is referable to B. virilis reflects the fact that the larvae of B. virilis are sexually dimorphic (Ide 1937). Differ- ences in the male and female adults of B. virilis were first noted by McDunnough (1924). Acerpenna sp. 1 McCafferty and Davis [= Acerpenna pygmaea (Hagen)]. McCafferty and Davis (1992) stated that this species was similar to A. harti (McDunnough). We now interpret A. sp. 1 as an adult variant of the relatively widespread species A. pygmaea. Baetis sp. 1 McCafferty and Davis [= Baetis punctiventris (McDunnough)]. These larvae represent a pale color variant of B. punctiventris that lacks the ventral spotting usually typical of the species. It is the most common two- tailed Baetis in the Hill country of Texas, where we have additionally seen it from Medina River, Bandera County; Guadalupe River, Comal County; Blanco River, Hays County; Block Creek, Kendall County; and Gorman Creek, San Saba County. Camelobaetidius sp. 1 McCafferty and Davis. This species was originally reported from larvae from the Rio Grande River along the Texas/Mexico bor- der by McCafferty and Davis (1992), and more recently larvae were reported from Colorado by McCafferty et al. (1993). Formal description of a new spe- cies has been postponed until more mature specimens are available. Centroptilum sp. 1 McCafferty and Davis [= Centroptilum triangulifer (McDunnough)]. McCafferty and Davis (1992) initially noted the similarity of C. sp. 1 to C. triangulifer. Observations of variation in this species since that time indicate that the Texas material falls within the range of variation, and C. triangulifer should be added to the list of Texas mayflies. Centroptilum sp. 3 Lugo-Ortiz and McCafferty. The single larva that the report of Lugo-Ortiz and McCafferty (1995) was based on was sent to another co-worker for an opinion as to its identification and has since been lost. Thus, we presume that it will never be resolved. Procloeon viridoculare (Berner), which was recently reported from Texas by Wiersema (1998), has been taken from the same locality as C. sp. 3, on the South Llano River in Kimble County, but we cannot be sure of any association. Procloeon sp. 1 McCafferty and Davis. Due primarily to the fact that this report was based on female adults, the material still cannot be placed to any nominal species. Procloeon sp. 2 McCafferty and Davis [= Centroptilum album McDun- nough]. Centroptilum album was first reported by Moore (1950) from Texas (San Marcos River); however, that report generally has not been referenced in subsequent inventories of mayflies in Texas. We still cannot verify the accu- racy of Moore's report, but must assume it was correct, based on the now confirmed presence of C. album in Texas. Procloeon sp. 2 was based on larvae that fall within the expected range variation of C. album, as had been sug- 1 1 6 ENTOMOLOGICAL NEWS gested by McCafferty and Davis (1992). This species has been placed both in Centroptilum and Procloeon recently; however, it is more consistent with the concept of Centroptilum reflected in the key to North American mayfly genera by Edmunds and Waltz ( 1 996). Procloeon sp. 3 McCafferty and Davis. This new but unnamed species was based on highly distinctive larvae from the J. R. Davis private collection taken from Pinto Creek, Kinney County Texas (see McCafferty and Davis 1992). While we were in the process of writing up the formal new description and new name for the McCafferty and Davis species for publication, we learned that other workers had also decided to describe this species independently. Rather than cause an unnecessary synonym in the literature, we are deferring to those others, although to our knowledge a formal description is not in press at this time, nor can we be sure when or if it will be published in the near future. ACKNOWLEDGMENTS We thank C. R. Lugo-Ortiz and R. P. Randolph (West Lafayette, Indiana) for discussing and reviewing this paper. LITERATURE CITED Edmunds, G. FM Jr. and R. D. Waltz. 1996. Ephemeroptera. Pp. 126-163 In: R. W. Merritt and K. W. Cummins (eds.), An introduction to the aquatic insects of North America, 3rd edition. Kendall/Hunt, Dubuque. Ide, F. P. 1937. Descriptions of eastern North American species of baetine mayflies with particu- lar reference to the nymphal stage. Can. Entomol. 69: 219-213, 235-243. Jacob, U. and A. Glazaczow. 1986. Pseudocentroptiloides, new baetid genus of Palearctic and Oriental distribution (Ephemeroptera). Aq. Insects 8: 197-206. Keffermuller, M. and R. Sowa. 1984. Survey of central European species of the genera Centroptilum Eaton and Pseudocentroptilum Bogoescu (Ephemeroptera: Baetidae). Polsk. Pismo Entomol. 54: 309-340. Lugo-Ortiz, C. R. and W. P. McCafferty. 1995. The mayflies (Ephemeroptera) of Texas and their biogeographic affinities. Pp. 151-169 In: L. Corkum and J. Ciborowski (eds.), Current directions in research on Ephemeroptera. Can. Schol. Press, Toronto. McCafferty, W. P. and J. R. Davis. 1992. New and additional records of small minnow mayflies (Ephemeroptera: Baetidae) from Texas. Entomol. News 103: 199-209. McCafferty, W. P. and R. D. Waltz. 1990. Revisionary synopsis of the Baetidae (Ephemeroptera) of North America. Trans. Am. Entomol. Soc. 1 16: 769-799. McCafferty, W. P., R. S. Durfee, and B. C. Kondratieff. 1993. Colorado mayflies (Ephe- meroptera): an annotated inventory. Southwest. Nat. 38: 252-274. McDunnough, J. 1924. New Canadian Ephemeridae with notes, II. Can. Entomol. 56: 90-98, 113-122, 128-133. Moore, L. F., Jr. 1950. Distribution of mayfly nymphs in streams of Dallas County, Texas. Field Lab. 18: 103-112. Waltz, R. D. and W. P. McCafferty. 1989. New species, redescriptions, and cladistics of the genus Pseudocentroptiloides (Ephemeroptera: Baetidae). J. New York Entomol. Soc. 97: 151- 158. Wiersema, N. A. 1998. Newly reported and little known mayflies (Ephemeroptera) from Texas. Entomol. News, 109: 27-32. Vol. 109, No. 2, March & April, 1998 117 THE ADULT OF MORIBAETIS MACAFERTI (EPHEMEROPTERA: BAETIDAE)1 W. P. McCafferty, C. R. Lugo-Ortiz2 ABSTRACT: Alate stages of the common and distinctive Central American and southern Mexi- can species Moribaetis macaferti are described for the first time based on males of both the adult and subimago stages. The species is distinct as an adult in having forewings with extensive stain- ing, an abdomen with distinctive patterning, and genital forceps with a smooth medial margin. The species' abdominal color pattern is also apparent in the subimago. A key to the male adults of Moribaetis species is provided. Moribaetis macaferti is reported from Panama for the first time. The distinctive mayfly genus Moribaetis was described by Waltz and McCafferty (1985). It was later restricted by Lugo-Ortiz and McCafferty (1996a) and shown to be a monophyletic, relatively ancestral lineage within the New World Baetodes complex of genera, which also includes the genera Baetodes Needham and Murphy, Mayobaetis Waltz and McCafferty, and Prebaetodes Lugo-Ortiz and McCafferty. Moribaetis is Neotropical in origin (McCafferty et al. 1992), and the entire Baetodes complex is evidently South American in origin (McCafferty 1998), although several species of Baetodes are found in the southwestern Nearctic region. Moribaetis essentially is a Central American genus. It currently consists of M. macaferti Waltz, M. maculipennis (Flowers), and M. salvini (Eaton). Men- tion of the genus as occurring in South America (Lugo-Ortiz and McCafferty 1996a) was inadvertent, although an unspecified report of the genus by Rojas de Hernandez et al. (1995) could actually be applicable to either Moribaetis or Mayobaetis because they were considered subgenera of Moribaetis at the time. The latter may be more likely because Mayobaetis is known from Ecuador and Peru as well as Central America. Moribaetis macaferti has recently been reported from southern Mexico in addition to Costa Rica, Honduras, and Guatemala (Lugo-Ortiz and McCafferty 1994, 1996b). This is a relatively large, striking species of Baetidae that has been collected by WPM from streams and waterfalls, including splash zones, in Costa Rica. A key to the larvae of all three species of Moribaetis was pro- vided by Waltz and McCafferty (1985). Although M. maculipennis and M. salvini are known as adults (Eaton 1885, Flowers 1979), the adult of M. macaferti has remained unknown up to this time. Boris Kondratieff (Fort Collins, Colorado) has kindly given us larvae and an adult of M. macaferti that he recently collected from Puebla, Mexico in 1 Received August 2, 1997. Accepted September 2, 1997. 2 Department of Entomology, Purdue University, West Lafayette, IN 47907, USA. ENT. NEWS 109(2) 1 17-121, March & April, 1998 1 1 8 ENTOMOLOGICAL NEWS 1994. In addition, Wills Flowers (Tallahassee, Florida) has kindly loaned us larvae and a reared subimago of M. macaferti that represent a new country record (see material examined, below). We are thereby able to provide a first description of the adult M. macaferti and the first species key to male adults of Moribaetis. Moribaetis macaferti Waltz Male adult. Dimensions in mm: body length, 9.7; forewing length, 7.6; forewing width, 3.5; hindwing length, 1.2; caudal filaments length, 20.0. Head (Figs. 1 and 2): Coloration generally light yellowish brown. Turbinate portion of compound eyes smoke yellow, nearly round with anterior-posterior length only slightly longer than width, nearly contiguous, and mounted on short stalks with encircling band of suffused black. Scape and pedicel of antennae distally marked with brown. Thorax (Figs. 1 and 3): Pronotum (Fig. 1 ) cream medially and light brown laterally. Antecosta of mesotergum (Fig. 3) suffused dark brown. Meso- and metanota cream with medium and light brown elongated markings as illustrated in Figure 3, including conspicuous narrow- elongate U-shaped submedian medium brown mark on scutum. Forefemora light to medium brown; foretibiae medium brown, with length 1 .32x that of femora; foretarsal segment 1 medium brown; foretarsal segments 2-5 cream basally and light brown distally. Mid- and hindlegs cream with femora and tarsi light brown distally; tibiae subequal in length to femora. Claws dissimilar, each consisting of one hooked-acute and one straight-ovoid member. Forewings with distinct markings as illustrated in Figure 4; yellowish brown stains generally associated with crossvenation but more extensive in basal area of membrane with large free spots in basal costal and subcostal area and extensions from crossveins near MA2 and M?2- Hindwings as in Figures 5 and 6; membrane devoid of staining; costal process acute and asymmetrical (straight edged posteri- orly); first elongate vein unforked; second elongate vein forked in distal half, with forks encom- passing free marginal intercalaries; third elongate vein short, terminating in anal margin. Abdo- men (Fig. 7): Terga light basally, becoming more suffused with yellowish light brown shading posteriorly (terga 6-10 markedly becoming progressively more shaded); tergum 1 with narrow lateral medium brown markings; terga 2-8 with additional triangular medium brown markings extending length of terga and filling posterolateral corners of terga; tergum 9 with lateral mark- ings more extensive anteriorly and not well developed posteriorly, with additional pair of small oblique dashes sublaterally at posterior margin; tergum 10 pale and unmarked. Sterna 1-6 whit- ish, almost translucent; sterna 7-9 pale yellow, almost translucent; sternum 9 pale yellow. Geni- talia (Fig. 8) with short, medially smooth basal segment, elongate second segment, and small, rounded terminal segment weakly demarcated from second segment. Caudal filaments, for most of length, distinctly banded in alternating pattern of one white and three darkened segments. Male subimago. Generally similar to adult. Turbinate portion of compound eyes appearing slightly more oval-elongate. Color pattern not well developed on body and not yet evident in forewings as described above. Submedian elongate marking on thoracic scutum not formed into U-shape, but unconnected posteriorly. Subcostal process of hindwings similar to adults but appearing mi- nutely hooked, probably as an artifact of the subimaginal pellicle; only one major intercalary evident between forks of second elongate vein. Triangular posterolateral abdominal tergal mark- ings evident but not highly contrasting as in adult. Genitalia and caudal filaments missing. Material examined. MEXICO, PueblaSta, Pahuatlan, Hwy 106, VIII- 17- 1994, B. C. Kondratieff, male adult and associated larvae (left fore- and hindwings slide mounted). PANAMA, Bocas del Toro Prov., trib. of Rio Guabo at pipeline rd., V-28-1985, R. W. Flowers (male subimago and associated larvae). Vol. 109, No. 2, March & April, 1998 119 Figs. 1-8. Moribaetis macaferti, male adult. 1. Head and pronotum. 2. Head (lateral view). 3. Meso- and metanota. 4. Left forewing. 5. Left hindwing (to scale with forewing). 6. Left hindwing (enlarged). 7. Abdomen (lateral view). 8. Male genitalia (ventral view). DISCUSSION The adult male described above was not reared; however, we have no doubt that it is M. macaferti. It was taken where only M. macaferti larvae were taken (B. C. Kondratieff, pers. comm.), is specifically distinct from other adults in the genus, and essentially agrees with the subimago from Panama that was indeed reared from larvae of M. macaferti (R. W. Flowers, pers. comm.). The forewings of M. macaferti have more extensive staining than those of M.maculipennis [see Flowers (1979): Fig. 1] and M. salvini [see Eaton (1885): Fig. 29a]. The hindwings are most similar to those of M. maculipennis in vena- tion but differ in not having any staining [see Flowers (1979): Figs. 2 and 3]. It is possible that the number of marginal intercalaries between the forks of the second elongate vein in the hindwings is variable within M. macaferti, based on a comparison of the hindwings of the subimago from Panama and the adult from Mexico, and thus we do not recommend attempting to identify adults of species of Moribaetis on that basis. The abdominal terga of M. macaferti (Fig. 120 ENTOMOLOGICAL NEWS 7) possess distinct posterolateral markings on segments 2-8, and additionally lack the distinctive large brown submedian markings of M. maculipennis [see Flowers (1979): Fig. 5] and the pitch-black posterior marginal markings of the terga described by Eaton (1885). There are, however, very slight and difficult to detect indications of a pair of pale spots on most abdominal terga in M. macaferti. The terminal segment of the genital forceps of M. macaferti (Fig. 8) is most similar to that of M. maculipennis [see Flowers (1979): Fig. 4] in that it is weakly demarcated from segment 2. However, M. macaferti lacks the roughened medial margin of the basal segment of the forceps that is present in both M. maculipennis and M. salvini [see also Kimmins (1934): Fig. 13], in addition to the well-defined rounded and setose medioapical protrusion of the basal forceps segment seen in M. maculipennis [Flowers (1979): Fig. 4]. Key To Male Adults ofMoribaetis Species [key to larvae in Waltz and McCafferty (1985)] 1. Forewings with extensive staining throughout membrane (Fig. 4); abdominal terga 2-8 with triangular posterolateral markings (Fig. 7); forceps with medial margin of basal segment smooth medially (Fig. 8) M. macaferti I1. Forewings [Flowers (1979): Fig. 1; Waltz and McCafferty (1985): Fig. 43] and abdominal terga not as above; forceps [Kimmins (1934): Fig. 13; Flowers (1979): Fig. 4] with medial mar- gin of basal segment roughened 2 2. Cerci banded with two to three dark segments alternating with two light segments [Waltz and McCafferty (1985): Fig. 33] M. maculipennis 2'. Cerci banded with one slightly darkened segment alternating with three light segments [Waltz and McCafferty (1985): Fig. 42] M. salvini ACKNOWLEDGMENTS We thank R. W. Flowers (Florida A & M Univ.) and B. C. Kondratieff (Colorado State Univ.) for collecting and providing the specimens that have made this paper possible. The paper has been assigned Purdue Agricultural Research Program Journal No. 15467. LITERATURE CITED Eaton, A. E. 1883-88. A revisional monograph of recent Ephemeridae or mayflies. Trans. Linn. Soc. Lond., 2nd Ser. Zool. No. 3, 352 pp. Flowers, R. W. 1979. A new species of Baetis from Panama (Ephemeroptera: Baetidae). Pan- Pac. Entomol. 55: 187-191. Kimmins, D. E. 1934. Notes on the Ephemeroptera of the Godman and Salvin collection, with descriptions of two new species. Ann. Mag. Nat. Hist., Ser. 10. 14: 338-353. Lugo-Ortiz, C. R. and W. P. McCafferty. 1994. New records of Ephemeroptera from Mexico. Entomol. News 105: 17-26. Lugo-Ortiz, C. R. and W. P. McCafferty. 1996a. Phylogeny and classification of the Baetodes complex (Ephemeroptera:Baetidae), with description of a new genus. J. N. Am. Benthol. Soc. 15:367-380. Vol. 1 09, No. 2, March & April, 1 998 121 Lugo-Ortiz, C. R. and W. P. McCafferty. 1996b. New Central American and Mexican records of Ephemeroptera species. Entomol. News 107: 303-310. McCafferty, W. P. 1998. Ephemeroptera and the great American interchange. J. N. Am. Benthol. Soc., in press. McCafferty, W. P., R. W. Flowers and R. D. Waltz. 1992. The biogeography of Mesoamerican mayflies, pp. 173-193 In: S. P. Darwin and A. L. Weldon(eds.). Biogeography of Mesoamerica: proceedings of a symposium. Tulane Stud. Zool. Bot, Suppl. Publ. No. 1. Rojas de Hernandez, A. M., M. del Zuniga de Cardoso, M. H. Burbano T. and C. Serrato H. 1 995. Altitudinal distribution of Ephemeroptera in the Farallones de Cali National Park, Co- lombia, pp 121-129 In: L. D. Corkum and J. J. H. Ciborowski (eds.). Current directions in research on Ephemeroptera. Can. Scholars' Press, Toronto. Waltz, R. D. and W. P. McCafferty. 1985. Moribaetis: a new genus of Neotropical Baetidae (Ephemeroptera). Proc. Entomol. Soc. Wash. 87: 239-251. BOOKS RECEIVED AND BRIEFLY NOTED METHODS IN ECOLOGICAL & AGRICULTURAL ENTOMOLOGY. D.R. Dent & M.P. Walton, eds. 1997. CAB International. 387 pp. Citing the need for an up-to-date and comprehensive textbook on experimental and analyti- cal methods in both ecological and agricultural entomology, this book seeks to integrate new technologies and approaches to research with traditional methods, to provide a balanced view of the subject for final year undergraduates and postgraduates, and to be a useful source of refer- ence for research workers. Twenty three authors contributed chapters on thirteen subjects rang- ing from sampling, handling and rearing insects to studying insect behavior, quantifying insect populations and insect migration, host plant selection, insect pollination, insecticide efficacy and efficacy of natural enemies, and modelling. THE EVOLUTION OF MATING SYSTEMS IN INSECTS AND ARACHNIDS. J.C. Choe & B.J. Crespi, eds. 1997. Cambridge University Press. 387 pp. $44.95 pbk, $100 hdbk. The complex mixtures of conflict and cooperation that typify sex and sociality make these among the most endlessly fascinating and difficult topics in ecology and evolution. The twenty one chapters in this volume, contributed by thirty six authors, explore the intricacies of sexual and social competition and the variety of ways in which males and females pursue, persuade, manipulate and control, and help one another. This volume is intended to update the synthesis of insect mating systems by Thomhill and Alcock (1983) and complement the recent synthesis of the study of sexual selection by Andersson (1994). PROGRESS AND PROSPECTS IN EVOLUTIONARY BIOLOGY: THE DROSOPHILA MODEL. J.R. Powell. 1997. Oxford University Press. 562 pp. Cloth $70.00. The author states the primary impetus for this book is the conviction that if a major advance in understanding evolution is to be achieved, it is most likely to come from integration of levels of knowledge centered on a single closely related set of organisms. Drosophila has the greatest chance of being this organism. Thus, this book is about empirical studies of Drosophila. and is directed primarily toward graduate students but also to evolutionary biologists and colleagues studying development, molecular biology, and neurobiology of Drosophila. 1 22 ENTOMOLOGICAL NEWS FIRST REPORT AND NEW SPECIES OF THE GENUS CLOEODES (EPHEMEROPTERA: BAETIDAE) FROM AUSTRALIA1'2 C. R. Lugo-Ortiz, W. P. McCafferty3 ABSTRACT: Cloeodesfustipalpus, new species, and C. illiesi, new species, are described from larvae from eastern Australia. The two species represent the first report of Cloeodes from the continent. Cloeodes fustipalpus is distinguished by the irregular labral setation, clublike labial palps segment 3, and abdominal color pattern. Cloeodes illiesi is distinguished by the bifid right prostheca with a medially setose branch, reduced maxillary palps, medially bulbous labial palps segment 3, abdominal color pattern, and narrow-elongate gills. Numerous morphological char- acteristics indicate that C. fustipalpus and C. illiesi are most closely related to the Afrotropical C. inzingae and the Oriental C. longisetosus and C. soldani. Three biogeographic scenarios are discussed that would explain the world distribution of Cloeodes. Traver (1938) erected the genus Cloeodes (Ephemeroptera: Baetidae) for the Caribbean species C. maculipes Traver and C. consignatus Traver. The genus is distinct among small minnow mayflies because its larvae have eden- tate tarsal claws (Fig. 6; Waltz and McCafferty 1987b: Fig. 8), a conspicuous subproximal arc of long, fine, simple setae on the tibiae (Fig. 6; Waltz and McCafferty 1987b: Fig. 7), and setal tufts on sterna 2-6 (Waltz and McCafferty 1987a: Fig. 5; Waltz and McCafferty 1987b: Figs. 9, 44). Adults of Cloeodes are distinguished by having segment 2 of the male genital forceps basally bul- bous and with abundant minute, fine, simple setae (Waltz and McCafferty 1987b:Fig. 34). Cloeodes has been reported from the Afrotropics, Neotropics, Orient, and southwestern Nearctic (Traver 1938, Waltz and McCafferty 1987ab, 1994, Kluge 1991, Flowers 1991, Lugo-Ortiz and McCafferty 1993, 1994, 1995, McCafferty and Lugo-Ortiz 1995, McCafferty et al. 1997). Herein we report Cloeodes for the first time from Australia. The report is based on two new species described from larvae collected from Queensland and New South Wales. The specimens studied are housed in the Purdue Entomological Research Col- lection, West Lafayette, Indiana. The discovery of Cloeodes in Australia is of considerable biogeographic interest because it is the first genus of Baetidae known to occur throughout the Southern Hemisphere. Cloeodes may have been widespread in Gondwanaland during the Jurassic approximately 1 80 million years ago (mya). Southern Hemi- sphere landmasses drifting to their present positions would have carried an- 1 Received July 28, 1997. Accepted August 27, 1997. 2 Purdue Agricultural Research Program Journal No. 15460. 3 Department of Entomology, Purdue University, West Lafayette, IN 47907. ENT. NEWS 109(2) 122-128 , March & April, 1998 Vol. 109, No. 2, March & April, 1998 123 cestral species of Cloeodes with them. The South East Asian distribution could be explained by the northward drifting and abutment of the Indian subconti- nent approximately 45 mya. The southern Nearctic distribution of the genus has been explained by northward dispersal during and after the formation of the Isthmus of Panama approximately 6-5 mya, as reviewed by McCafferty (1998). Alternatively, Cloeodes may have originated somewhat later on the African-Indian-South American landmass (essentially West Gondwanaland) during the Early Cretaceous approximately 1 40 mya. This explanation is simi- lar to the first, except that dispersal of the genus from Asia into Australia dur- ing the Middle Miocene approximately 15 mya is assumed. A third historical explanation would have Cloeodes originating in Africa-South America during the Middle Cretaceous approximately 1 10-100 mya. At that time, however, the genus might not have been present on the Indian subcontinent, and the pres- ence of Cloeodes in the Orient, Australia, and North America would all be attributed to dispersal events beginning in the Eastern Hemisphere when Af- rica and Eurasia reunited during the middle Miocene approximately 17 mya. Interchange between Africa and Asia in this period is consistent with some other animal groups (e.g., see Cox and Moore 1985). We cannot at this time be sure which of these three biogeographic explana- tions is the most likely because only a small number of species of Cloeodes are known and cladistic analysis is not possible. Considerable insular evolution of the genus has occurred in South America since the isolation of that continent, as evidenced by the fact that Western Hemisphere Cloeodes are a closely re- lated, distinctive grouping (Waltz and McCafferty 1987b). The fact that all species in the Eastern Hemisphere are closely related to each other (see spe- cies discussions below) would further suggest that African, Asian, and Austra- lian lineages have dispersed relatively recently, not having been isolated from each other to the extent in which Western and Eastern Hemisphere lineages of the genus have. Cloeodes fustipalpus Lugo-Ortiz and McCafferty, NEW SPECIES Larva. Body length: 5.1-6.0 mm. Caudal filaments length: 2.3-2.5 mm. Head: Coloration light yellow-brown, with faint vermiform markings on vertex. Antennae approximately 1 .5x length of head capsule. Labrum (Fig. 1 ) with submedial pair of long, fine, simple setae and submarginal row of six to eight fine, simple setae of various lengths. Hypopharynx similar to Figure 1 1 . [Left and right mandibles (Figs. 2, 3) with outer incisors worn in material examined.] Left mandible (Fig. 2) with inner incisor with three denticles; prostheca robust, apically denticulate; minute denticles present between prostheca and mola. Right mandible (Fig. 3) with inner incisor with four denticles; prostheca somewhat slender, apically acute; minute denticles present between prostheca and mola. Maxillae (Fig. 4) with three long, fine, simple setae near medial hump; maxillary palps reaching galealaciniae; palp segment 1 approximately 0.50x length of segment 2. Labium (Fig. 5) with glossae and paraglossae equal in length; palp segment 1 approximately O.SOx length of segments 2 and 3 combined; segment 2 approximately 1 .20x length of segment 3; 1 24 ENTOMOLOGICAL NEWS segment 3 bulbous, clublike (medially broader than apical width of segment 2). Thorax: Colora- tion pale yellow-brown, with complex markings. Hindwingpads absent. Legs (Fig. 6) cream; femora with dorsal row of five to eight long, robust, simple setae, last two almost contiguous and longer than others; tibiae with dorsal row of long, fine, simple setae; tarsi with dorsal row of long, fine, simple setae. Abdomen (Fig. 7): Coloration pale brown and cream; segment 1 cream; segment 2 pale brown, with submedial and sublateral pairs of large, oblong, cream markings; segment 3, 5, and 6 pale brown, with submedial pair of circular cream markings; segment 4 anteriorly and posteriorly pale brown, medially cream; segment 7 anteriorly pale brown, posteri- orly cream; segment 8 cream; segment 9 pale brown, with semicircular anteromedial cream mark- ing; segment 10 pale brown. Sterna cream. Gills (Fig. 8) subtriangular, well tracheated, with smooth margin. Paraprocts (Fig. 9) with 18-20 sharp marginal spines, increasing in size apically; abundant scale bases scattered over surface. Caudal filaments whitish; medial caudal filament approximately O.SOx length of cerci. Adult. Unknown. Material examined. Holotype: Larva, AUSTRALIA, New South Wales, Chandler R., 26 mi E of Armidale, no date, G. F. Edmunds. Paratypes: Larva, same data as holotype [mouthparts, left foreleg, gill 4, and paraproct mounted on slide (medium: Euparal)]; two larvae, AUSTRA- LIA, New South Wales, Bellinger R., at Bellinger, 11-23-1966, G. F. Edmunds. Additional mate- rial: Three exuviae, AUSTRALIA, New South Wales, Serpentine R., New England National Park, 11-19-1966, G. F. Edmunds; three larvae, AUSTRALIA, New South Wales, Bellinger R., at Bellinger, 11-23-1966, G. F. Edmunds. Etymology. The specific epithet is a combination of the Latin words fustis (club) and palpus (palps). It is in reference to the clublike labial palps. Discussion. Cloeodes fustipalpus is distinguished from other members of the genus by the irregular setation of the labrum (Fig. 1), clublike segment 3 of the labial palps (Fig. 5), and abdominal color pattern (Fig. 7). The abdominal color pattern varies somewhat among specimens, but the most consistent is the one shown in Figure 7. Cloeodes fustipalpus appears to be related to the Oriental species C. longisetosus (Braasch and Soldan) and C. soldani (Muller-Liebenau), the Afrotropical species C. inzingae (Crass), and C. illiesi, new species, from Aus- tralia (see below). The larvae of all these species have a bulbous labial palp segment 3 (Figs. 5, 15; Crass 1947: Fig. 9a; Braasch and Soldan 1980: Fig. 12; Muller-Liebenau 1983: Fig. 3b), well-developed rows of long, fine, simple setae on the tibiae and tarsi (Fig. 6; Braasch and Soldan 1980: Figs. 2, 3; Waltz and McCafferty 1994: Fig. 2), and lack hindwingpads. Cloeodes illiesi Lugo-Ortiz and McCafferty, NEW SPECIES Larva. Body length: 3.9 mm. Caudal filaments length: unknown. Head: Coloration yellow- brown to medium brown, with faint vermiform markings on vertex. Antennae approximately 1.5x length of head capsule. Labrum (Fig. 10) with submedial pair of long, simple setae and submarginal row of three to four long, fine, simple setae. Hypopharynx as in Figure 11. Left mandible (Fig. 12) with six denticles; prostheca robust, apically denticulate; minute denticles between prostheca and mola absent. Right mandible (Fig. 13) with outer incisor with four den- ticles; inner incisor with three denticles; prostheca slender, bifid, one branch medially with minute, fine, simple setae; minute denticles between prostheca and mola absent. Maxillae (Fig. 14) with Vol. 109, No. 2, March & April, 1998 125 Figs. 1-9. Cloeodesfustipalpus, new species, larva. 1. Labrum. 2. Left mandible. 3. Right man- dible. 4. Right maxilla. 5. Labium (left-ventral; right-dorsal). 6. Left foreleg. 7. Abdomen (dor- sal). 8. Gill 4. 9. Paraproct. 126 ENTOMOLOGICAL NEWS three long, fine, simple setae near medial hump; maxillary palps not reaching galealaciniae; palp segment 1 approximately 0.60x length of segment 2. Labium (Fig. 1 5) with glossae and paraglossae equal in length; palp segment 1 as long as segments 2 and 3 combined; segment 2 approximately 0.74x length of segment 3; segment 3 bulbous, apically flattened. Thorax: Coloration pale to medium yellow-brown, with complex markings. Hindwingpads absent. Legs (similar to Fig. 6) cream; femora with dorsal row of five to seven long, robust, simple setae, last two almost con- tiguous; tibiae with dorsal row of long, fine, simple setae; tarsi with dorsal row of long, fine, simple setae. Abdomen (Fig. 16): Coloration pale brown and yellow-brown; segment 1 yellow- 15 16 18 Figs. 10-18. Cloeodes illiesi, new species, larva. 10. Labrum. II. Hypopharynx. 12. Left man- dible. 13. Right mandible. 14. Right maxilla. 15. Labium (left-ventral; right-dorsal). 16. Abdo- men (dorsal). 17. Gill 6. 18. Paraproct. Vol. 1 09, No. 2, March & April, 1 998 1 27 brown; segment 2 pale brown, with submedial anterior pair of large yellow-brown oblong mark- ings and sublateral oblong yellow-brown markings; segments 3-6 pale brown, with submedial anterior pair of small subtriangular yellow-brown markings; segment 7 pale brown; segment 8 yellow-brown; segment 9 pale brown, with faint medial streak; segment 10 pale brown. Sterna cream to yellow-brown. Gills (Fig. 1 7) narrow-elongate, poorly tracheated, with smooth margin. Paraprocts (Fig. 18) with 9-10 sharp marginal spines; scale bases scattered over surface. Caudal filaments whitish. Adult. Unknown. Material examined. Holotype: Larva, AUSTRALIA, Queensland Province, nr. Cairns, Cascade Falls, sea level, 20°C, X- 13- 1966, J. lilies [mouthparts, left foreleg, and paraproct mounted on slide (medium: Euparal)]. Etymology. This species is named after the late renowned entomologist- limnologist Joachim lilies, who collected it. Discussion. Cloeodes illiesi is distinguished from other members of the genus by the bifid right prostheca with a setose branch (Fig. 1 3), reduced max- illary palps (Fig. 14), medially bulbous labial palps segment 3 (Fig. 15), ab- dominal color pattern (Fig. 16), and narrow-elongate gills (Fig. 17). Its pos- sible relationships to other species of Cloeodes are discussed above under C. fustipalpus. ACKNOWLEDGMENTS We thank G. F. Edmunds, Jr. (Salt Lake City, Utah) for the donation of the material used in this study. LITERATURE CITED Braasch, D. and T. Soldan. 1 980. Centroptella n. gen., eine neue Gattung der Eintagsfliegen aus China (Baetidae, Ephemeroptera). Reichenbachia 18: 123-127. Cox, C. B. and P. D. Moore. 1985. Biogeography: an ecological and evolutionary approach, 4th edition. Blackwell Scientific Pub., London. Crass, R. S. 1947. The may-flies of Natal and the Eastern Cape. Ann. Natal Mus. 11: 37-1 10. Flowers, R. W. 1991. Diversity of stream-living insects in northwestern Panama. J. N. Am. Benthol. Soc. 10: 322-334. Kluge, N. 1991. Cuban mayflies of the family Baetidae (Ephemeroptera) 1. Genera Callibaetis, Cloeodes, and Paradoeodes. Zool. Zh. 12: 128-136. [in Russian] Lugo-Ortiz, C. R. and W. P. McCafferty. 1993. Genera of Baetidae (Ephemeroptera) from Central America. Entomol. News 104: 193-197. Lugo-Ortiz, C. R. and W. P. McCafferty. 1994. New records of Ephemeroptera from Mexico. Entomol. News 105: 17-26. Lugo-Ortiz, C. R. and W. P. McCafferty. 1995. Annotated inventory of the mayflies (Ephemeroptera) of Arizona. Entomol. News 106: 131-140. McCafferty, W. P. 1998. Ephemeroptera and the great American interchange.]. N. Am. Benthol. Soc., in press. 128 ENTOMOLOGICAL NEWS McCafferty, W. P. and C. R. Lugo-Ortiz. 1995. Cloeodes hydation, n. sp. (Ephemeroptera: Baetidae): an extraordinary, drought tolerant mayfly from Brazil. Entomol. News 106: 29- 35. McCafferty, W. P., C. R. Lugo-Ortiz, and G. Z. Jacobi. 1997. The mayfly fauna of New Mexico. Gr. Basin Natural., 57: 283-314. Miiller-Liebenau, I. 1983. Three new species of the genus Centroptella Braasch and Soldan, 1 980, from Sri Lanka (Insecta: Ephemeroptera). Arch. Hydrobiol. 97: 486-500. Traver, J. R. 1938. Mayflies of Puerto Rico. J. Agric. Univ. P. R. 22: 5-42. Waltz, R. D. and W. P. McCafferty. 1987a. Generic revision of Cloeodes and description of two new genera (Ephemeroptera: Baetidae). Proc. Entomol. Soc. Wash. 89: 177-184. Waltz, R. D. and W. P. McCafferty. 1987b. Revision of the genus Cloeodes Traver (Ephemeroptera: Baetidae). Ann. Entomol. Soc. Am. 80: 191-207. Waltz, R. D. and W. P. McCafferty. 1994. Cloeodes (Ephemeroptera: Baetidae) in Africa. Aq. Insects 16: 165-169. BOOKS RECEIVED AND BRIEFLY NOTED THE BIONOMICS OF GRASSHOPPERS, KATYDIDS AND THEIR KIN. S.K. Gangwere & M.C. & M. Muralirangan, eds. 1997. CAB International. 529 pp. This volume consists of twenty chapters authored by an international group of twenty eight scientists and is written from a broad, comparative biological, behavioral, and evolutionary ap- proach best expressed by the term bionomics. It focuses on history and recent developments in grasshopper and plague locust biology as well as the biology of katydids, crickets, and other Orthoptera, an insect group of exceptional economic and biological interest. INSECT ECOLOGY, 3rd ed. P.W. Price. 1997. J. Wiley & Sons. 874 pp. Moving from the dynamics of plant-insect interactions, predation, parasites and hosts, as well as mutualistic relationships, including pollination ecology, this book examines the themes central to understanding the role of insects in our environment. It describes the various levels of insect interaction, from trophic relationships, populations, and communities, while unfolding the infinite variety of insect species and their visible legacy in the fossil record. This new edition includes discussion on the nature of ecological theory and how it is advanced, the evolutionary perspectives on population dynamics, the existence and study of vacant ecological niches, latitu- dinal gradients in species richness, and conservation of biodiversity. Vol. 109, No. 2, March & April, 1998 129 DIEL EMERGENCE PATTERNS OF TRICORYTHODES STYGIATUS (EPHEMEROPTERA: LEPTOHYPHIDAE) ON THE LITTLE LEHIGH CREEK NEAR ALLENTOWN, PENNSYLVANIA1 Fran Cough, Bruce L. Haase^ ABSTRACT: Tricorythodes stygiatus subimagos and adults show a diel periodicity in drift rates. Most of their activity is centered around the hours of sunrise and sunset. There is a differential emergence of male and female subimagos, males emerge after sunset and females emerge after sunrise. Light periodicity is the primary influence in the timing of T. stygiatus emergence, molt- ing, and oviposition. This species is univoltine at this study site. Tricorythodes stygiatus McDunnough (Ephemeroptera: Leptohyphidae) is a common species in the limestone streams of southeastern Pennsylvania . The species emerges throughout the summer. Tricorythodes species have been de- scribed as univoltine (Newell and Minshall 1978), bivoltine (Hall 1975), or multivoltine (Newell and Minshall 1978) depending on geographic location and water temperatures. Tricorythodes species are atypical of most mayflies in that the subimago emerges from the larval shuck underwater and swims/floats/crawls to the sur- face, with males emerging after dark and females emerging early the next morning (Hall 1975; Edmunds and McCafferty 1988). In Hall's (1975) study of T. allectus Needham, the surface drifting of male subimagos took place from sunset until the early hours of the morning, while female subimagos showed a peak in surface drift rate around sunrise. The emergence of male subimagos of T. allectus was correlated with low light inten- sity in the evening, while female subimago emergence is correlated with in- creasing light intensity in the morning (Hall 1975). However, in a study by Newell and Minshall (1978) T. minutus always emerged at the water surface, usually in the afternoon. T. minutus seems to be little influenced by photope- riod. T. allectus male subimagos molt to adults before dawn, so the length of the male subimago stage is 5-7 hours. The female's subimago stage is very short. Some female subimagos molt immediately after emergence, others may wait up to 2 hours (Hall 1975). The male alate life of T. allectus lasts about 9-10 hours, beginning after dusk until the sun is well above the horizon. The female's alate stage spans a few short hours after dawn (Hall, Berner and Cook 1975). 1 Received June 9, 1997. Accepted September 20, 1997. 2 Department of Biological Sciences, East Stroudsburg University, East Stroudsburg, Pa. 1 830 1 . ENT. NEWS 109(2) 129-135 , March & April, 1998 130 ENTOMOLOGICAL NEWS In T. minutus, the subimago stage usually lasts less than 30 minutes and the alate forms live less than 6 hours (Newell and Minshall 1978). The purpose of this study is to determine if there is a differential emer- gence of male and female subimagos of T. stygiatus in the Little Lehigh Creek in Allentown, PA., and to determine if this differential emergence can be cor- related with water temperature or time of day. METHODS This study took place on the Little Lehigh Creek, a 4th order stream in Salisbury Township, Lehigh County, Pennsylvania. The study site was at the end of a riffle in the main channel of the stream. It is a limestone spring creek with a typical riffle-pool complex, with a substrate composed mainly of marl and silt. Sampling was done using a Munro style drift net with a 250 micron mesh size and a net opening measuring 39cm x 39cm and a length of approximately one meter. A removable (250 cm) catch basket, composed of 3 inch PVC pipe and mesh screen was attached to the end of the drift net. The frame, made of 0.5 inch PVC pipe, was slid over steel reinforcing rods that were hammered into the stream bottom. When in place, the Munro drift net extended from the stream bottom through the water's surface (anywhere from 5 cm to 15 cm above the surface depending on water levels). The net was placed in the selected riffle of the study area for 24 hours at a time. Samples were removed and the net replaced every hour during the study period. At this time, the stream temperature was also taken and recorded. These 24 hour study periods were conducted three times, about one month apart, on July 1 3, August 1 1 , and September 8 of 1995. The samples were rinsed from the net into a pan, concentrated with an aquarium net and preserved in 70% ethanol. Using a 20x Swift dissecting mi- croscope, these insects were counted, aged (as subimagos or adults) and the subimagos sexed. To determine the life stage of the alate T. stygiatus, the meth- ods described by Edmunds and McCafferty (1988) were used. Subimagos had translucent wings with cilia along the hind edge. Adults had transparent wings without cilia on the hind edge of the wing. Subimagos and adults were sexed as males if claspers were present at the end of the abdomen, and as females if claspers were absent. Voucher specimens are deposited at the Department of Biological Sciences, East Stroudsburg University, East Stroudsburg, Pa. RESULTS The largest numbers of alate T. stygiatus were caught in the July 1995 sample with 891 organisms collected, including 73 subimagos and 818 adults. During the July 13th sample period, the water temperatures ranged from 16° C. in the early morning hours to 21° C. in the late afternoon. Sunrise was at Vol. 109, No. 2, March & April, 1998 131 0542 and sunset was 2033 so the day length was 14 hours 51 minutes. The peak drift time for male subimagos occurred between 2200 and 2400; for female subimagos this peak occurred between the hours of 0600 and 0900 (Table 1 , Figure 1 ). Adults showed a peak drift rate between the hours of 0800 and 1 100 (Table 1, Figure 2). Fifty- four alate T. stygiatus were collected in the August drift samples, including 8 subimagos and 46 adults. During the August 1 1th sample period, the water temperatures ranged from 15° C. in the early morning hours to 19° C. in the late afternoon. Sunrise was at 0608 and sunset was 2006, so the day length was 13 hours and 58 minutes. The peak drift time for male subimagos occurred between 2100 and 2300, for female subimagos this peak occurred between 0900 and 1000 (Table 1, Figure 1). Adults showed a peak drift rate between the hours of 0900 and 1000 (Table 1 , Figure 2). Table 1 . Hourly drift rates of Tricorythodes stygiatus subimagos and adults taken over a 24 hour period in the Little Lehigh Creek. Time 0100 0200 0300 0400 0500 0600 0700 0800 0900 1000 1100 1200 1300 1400 1500 1600 1700 1800 1900 2000 2100 2200 2300 2400 Total Subimago Male Subimago Female Adults 7/13/95 8/11/95 9/08/95 7/13/95 8/11/95 9/08/95 7/13/95 8/11/95 9/08/95 1 1 0 0 1 0 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0 8 4 11 28 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 5 0 1 0 6 0 1 0 0 0 0 0 0 0 0 0 20 0 4 0 4 0 15 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 1 0 0 0 0 0 0 0 2 45 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 1 1 1 6 0 59 1 16 1 349 11 7 0 335 22 16 0 63 3 4 0 2 1 1 0 1 0 0 0 0 1 0 0 2 0 1 0 0 1 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 1 1 0 0 3 0 0 2 818 46 52 132 ENTOMOLOGICAL NEWS Fifty-five alate T. stygiatus were collected in the September drift samples, including 4 subimagos and 51 adults. During the September 8th sample pe- riod, the water temperatures ranged between 15° C. and 16° C. Sunrise was at 0636 and sunset was 1921, so the day length was 12 hours 45 minutes. The peaks in drift time for male subimagos were at 1800 and 2100, for female subimagos these peaks were at 0700 to 0900 (Table 1 , Figure 1 ). Adults showed a peak in drift rate from 0700 to 1 100 (Table 1 , Figure 2). Number of Subimagos 7/13/95 8/11/95 Date 9/08/95 2400 Figure 1A. Hourly drift rate of Tricorythodes stygiatus male subimagos in the Little Lehigh Creek. Number of Subimagos 7/13/95 8/11/95 Date 9/08/95 2400 Figure IB. Hourly drift rate of Tricorythodes stygiatus female subimagos in the Little Lehigh Creek. Vol. 109, No. 2, March & April, 1998 133 400 350 300 250 Number of Adulti 200 150 100 50 1 Q — B O D D IB a n a fa) — a o — e 0600 1200 Time 1800 2400 Figure 2. Hourly drift rate of Tricorythodes stygiatus adults in the Little Lehigh Creek. DISCUSSION Alate forms of T. stygiatus adults on the Little Lehigh show a diel period- icity in their drift rates. Water temperature did not appear to trigger the differ- ential emergence of T. stygiatus in this study. The temperature of the stream did not fluctuate much (+ 2° C.) on a 24 hour basis, or on a seasonal basis ( 1 5°- 21° C.) throughout the study period. Instead, the peak for subimago and adult drift revolves around the hours of sunrise and sunset. This can be seen in the data as the season progresses and the day length shortens. It is apparent that light periodicity is the primary influence in the timing of the T. stygiatus alate life activities of emergence, molting, mating and oviposition. This agrees with the findings of Hall (1975) and Friesen, Flannagan and Lautersweiler (1980) (T. allectus). There was differential emergence of male and female subimagos of T. stygiatus in the Little Lehigh. This is similar to what Hall (1975), Friesen, Flannagan and Lautersweiler ( 1 980) and Edmunds and McCafferty ( 1 988) have found in other parts of North America for other species of Tricorythodes. In 134 ENTOMOLOGICAL NEWS found in other parts of North America for other species of Tricorythodes. In this study 83% (30 of 36) of the male subimagos emerged in the first 3 hours after sunset, and 93% (43 of 49) of the female subimagos emerged in the first 3 hours after sunrise. About 98% (894 of 915) of the spent adults were col- lected from 1 to 5 hours after sunrise, so mating and ovipostion had occurred prior to this event. Mating and oviposition occur for about 3-4 hours after sunrise based on the first/last appearance of spent wing adults in the drift samples. This is a somewhat longer period than was observed by Hall, Berner and Cook (1975) in T. allectus. These findings were similar to R. J. Hall's ( 1 975) study of T. allectus except that male subimago emergence did not occur steadily through the night, while the peak emergence of female subimagos occurred after sunrise. The short life span of Tricorythodes species has been well documented (Hall 1975; Hall, Berner and Cook 1975; Edmunds and McCafferty 1988). On the Little Lehigh, alate males live 8-14 hours and alate females live 2-5 hours on average, based on the time from first/last appearance of subimagos and the first/last appearance of adults. It appears that the T. stygiatus on the Little Lehigh Creek are univoltine based on this study. This would agree with Newell and Minshall's (1978) study on T. minutus. The drift rate of alate T. stygiatus was many times greater in July than in August or September. In this study, 89% (891 of 996) of the total drift is accounted for in the July sample alone. In a number of the T. stygiatus larvae, subimagos and adults, a distinct red coloration on the thorax was noted. In the 0600 hour sample on July 13, 1995, 70% of the female subimagos had a red thorax. The cause of this red colora- tion is another area for further investigation. A number of causes have been hypothesized from 1) an element of water chemistry (Gary Borger personal communication 1995), 2) parasitic mites (Greg Hoover personal communica- tion 1996, Brittain 1982), to 3) a type of polymorphism (Peters & Peters 1977). ACKNOWLEDGMENTS We would like to acknowledge the following people who helped make this project a success: for much needed assistance from Rod Rohrbach, Rich Heiserman, Steve Hoovler, Joe Kohler, Gary Pyle and other members of the Little Lehigh Fly Fishers and Little Lehigh Trout Unlimited; to Andy Shiels of the PA Fish and Boat Commission and Don Marushak of the Allentown Parks Dept., to Jim Munro for his drift nets; to Mark Gerber for his word processing skills; and the primary author gives special thanks to his wife, Maura, for her endless patience and understand- ing. The identification of this species as T. stygiatus was verified by Don Baylor of Aquatic Resource Consulting of Saylorsburg, PA and by Greg Hoover, Penn State University Department of Entomology. The authors would like to thank R.D. Waltz and W.P. McCafferty for their sug- gestions in reviewing this article. Vol. 109, No. 2, March & April, 1998 135 LITERATURE CITED Brit lain, J. E. 1 982. Biology of mayflies. Ann. Rev. Entomol. 27: 1 1 9- 1 47. Edmunds, G. F and W. P. McCafferty. 1988. The mayfly subimago. Ann. Rev. Entomol. 33: 509-529. Friesen, M. K., J. F. Flannagan and P. M. Laufersweiler. 1 980. Diel emergence patterns of some mayflies (Ephemeroptera) of the Roseau River (Manitoba, Canada). Advances in Ephemeropteran Biology . Plenum Publishing Co. p. 287-296. Hall, R. J. 1975. Life history, drift and production rate of the stream mayfly, Tricorythodes atratus McDunnough in the headwaters of the Mississippi River. Ph.D. Thesis, Univ. of Min- nesota. 288 pp. (T. allectus as T. atratus) Hall, R. J., L. Berner and E. F. Cook. 1975. Observations on the biology of Tricorythodes atratus McDunnough. Proc. Entomol .Soc. Wash. 77 (1): 34-49. Hall, R. J., T. F. Waters and E. F. Cook E. F. 1980. The role of drift dispersal in production ecology of a stream mayfly. Ecology 61 (1): 37-43. Newell, R. L., and G. W. Minshall. 1978. Life history of a multivoltine mayfly, Tricorythodes minutus: an example of the effect of temperature on the life cycle. Ann. Entomol. Soc. Am. 71: 876-881. Peters, W. L., and J. G. Peters. 1 977. Adult life and emergence ofDolania americana in north- western Florida. Int. Revue ges Hydrobiol. 62(3): 409-438. 136 ENTOMOLOGICAL NEWS THE FIRST FOSSIL DORYLINAE WITH NOTES ON FOSSIL ECITONINAE (HYMENOPTERA: FORMICIDAE)1 Mark B. DuBois 2> 3' 4 ABSTRACT: Two army ant species, Dorylus molestus and Neivamyrmex iridescens, are reported from copal of undetermined age (possibly Upper Pliocene and Pleistocene respectively). The presence of such fossils is remarkable given the disposition of many such species to foraging underground. Until more precise dates for this material are available, historical and biogeographic implications of these fossils remain uncertain. Ants are represented in the fossil record from Cretaceous through Pleis- tocene (Carpenter 1992; Bolton 1994). The currently recognized classification includes 16 extant subfamilies and four extinct subfamilies (Bolton 1994). A number of species are known from impressions in Tertiary shale (Carpenter 1930) and are mostly represented by reproductives. Many ants have been pre- served in amber (Wheeler 1914; Mayr 1868; Wilson 1985) and are mostly represented by workers. Foraging workers were trapped in resins which be- came amber, while reproductives flying over bodies of water were drowned and buried in silt (which later became shale). Some species, such as Stenamma berendti (Mayr), are known only from reproductives preserved in amber (Mayr 1868). Most publications have concentrated on pre-Pleistocene fossils; a few have dealt with sub-fossils (Francoeur and Elias 1985; DuBois 1993). Limited work has been done on specimens preserved in copal, which is usually Pleis- tocene or Pliocene in age (Poinar 1992, Larsson 1978). Most fossil worker ants belong to the extant subfamilies Formicinae, Dolichoderinae, Myrmicinae, and Ponerinae. Though some wing fragments have been tentatively identified as belonging to army ants, until recently, no fossil worker army ants were known. Wilson (1985) described Neivamyrmex ectopus from workers preserved in amber in the Dominican Republic. These fossils are thought to be late Oligocene or early Miocene in age. The rarity of fossil worker army ants is probably due to their behavior because many spe- cies principally forage underground. Recently, I had the opportunity to study fossil ants of both Ecitoninae and Dorylinae preserved in copal. Although copal is considered much younger in 1 Received Dec. 27 1996. Accepted January 31 1997. 2 1 16 Burton St., Washington, Illinois 61571-2509, U.S.A. 3 Research Affiliate, Center for Biodiversity, Illinois Natural History Survey, 607 East Peabody Drive, Champaign, Illinois 61820, U. S. A. 4 Send reprint requests to Washington, Illinois address. ENT. NEWS 109(2) 136-142, March & April, 1998 Vol. 109, No. 2, March & April, 1998 137 age (Recent, Pleistocene or Pliocene), these specimens provide a link (geo- logically and biogeographically) with older material. Both species are briefly discussed below. There appears to be a vast number of ant specimens preserved in copal from various deposits. The species discussed above represent a minuscule por- tion of available material. I hope this paper will stimulate further study of such fossils and subfossils. The precise age of the material discussed below is un- known (this is true for much copal). Although ages ranging from Recent through Pliocene have been proposed for various copal deposits, the stratigraphy of sites containing this material (and associations with micro-fossils) must be established to determine proper age. Dorylus molestus (Gerstacker) This species can be distinguished from related Dorylus species through the following combination of characters: head with convex sides; posterior angles of head pointed; ventral lobe of petiole well developed (Raignier and Van Boven 1955). Specimens appear identical to modern forms (as described in Raignier and Van Boven 1955). Bill Gotwald examined selected specimens and con- cluded they were this species ("... the common driver ant of East Africa, espe- cially Kenya and Tanzania.") (Gotwald, pers. comm.). Wheeler (1922: 740) gave the distribution of this species in Eastern Africa (including Mombassa, Mt. Kenia (2400 - 2800 m), Bura Mountains, Freretown, and Naivasha, Rift Valley). Two hundred and seventy one specimens contained in 12 pieces of copal were examined. This material was collected along the Tanzania - Kenya bor- der (Tanzania side, approximately 100 km south-west of Mombassa, Kenya). Individual pieces of copal were covered in caliche and found in a layer 1 1 m below the soil surface (Alan Graffham, pers. comm.). Specimen disposition is as follows. Lloyd Davis, Jr. provided two pieces of copal containing Dorylus. These have been returned to him. All remaining pieces discussed are from my personal collection and were obtained from Allen Graffham and other sources. Of these, one piece containing Dorylus has been donated to the British Mu- seum of Natural History and another piece containing Dorylus has been do- nated to the Museum of Comparative Zoology. Schluter and von Gnielinski (1987) indicated this copal was derived al- most entirely from resin of Hymenaea verrucosa (Leguminosae: Caesalpina- ceae). Deposition sites were listed as in the forest soil (primary), in the man- grove fringed estuary (secondary), and along the beach (tertiary). These deposits are part of the Mikindani beds (Stockley 1928). Stockley (1928) assigned an age of Upper Pliocene. Poinar (1992) indicated this Tanza- nian copal may be Pliocene in age (1.6-5 Ma). Schluter and von Gnielinski 1 38 ENTOMOLOGICAL NEWS (1987: 11) presented an age of Pleistocene, but cautioned that ". . . strati- graphically well defined associations of copal with index- or guide-fossils do not exist." von Gnielinski (pers. comm.) reiterated the uncertainty with the dating of copal from that region (Pleistocene or Pliocene could be argued). Additionally, Ken Anderson who is currently studying amber and copal depos- its from around the world (pers. comm.) indicated these were "resins of unde- termined geological age, but probably not of great antiquity." Schluter and von Gnielinski ( 1 987: 1 8) further stated: "Generally, it can be assumed that the fauna of the East African copal does not show significant differences from the fauna living today, i.e., all the trapped specimens repre- sent recent species..." Although a number of insects have been reported from these deposits (including nine species of Hymenoptera), no ants have previ- ously been reported (Schluter and von Gnielinski 1987). Figures 4 through 8 illustrate typical features of these specimens. Figures 1 1 and 12 show the general appearance of these specimens within the copal. Other arthropods were preserved with the ants. Their state of preservation, coupled with problems in identification of many East African insects, did not permit precise identifications. Specimens include other ants (several male Ponerinae, two male Myrmicinae, three worker Dolichoderinae, one minor worker Pheidole sp., one worker Crematogaster sp.), four Chalcidoidea, one alate termite, and a number of flies, beetles, leafhoppers, and spiders. After examining the fossils in cross section, it appears a foraging column of driver ants became trapped on the sticky resin and were then covered with another resin flow. Neivamyrmex iridescens Borgmeier This species can be distinguished from related Neivamyrmex species (Borgmeier 1955 - Group VII) through the following combination of charac- ters: postpetiole about five-sixths as long as high; head with violet reflections (difficult to see in fossil material); postpetiole about as long as wide, higher than long with posterior surface of node more steeply sloping than anterior surface; head smooth and distinctly shining; apex of scape closer to upper margin of head than eye level (Watkins 1976). Julian Watkins II identified the Neivamyrmex in one of these samples. He indicated the fossil is most similar to specimens of Neivamyrmex iridescens from Cundinamarca, Colombia in his collection. The only significant difference is a slightly longer than usual petiole. (Julian Watkins II, pers. comm.). Specimens key to N. iridescens using Watkins (1976) and fit the description provided by Borgmeier (1955: 540 - 542). This species is presently known from scattered localities: Panama, Guianas, Surinam, Bolivia, Colombia (Borgmeier 1955; Watkins 1976). Four specimens contained in two pieces of copal were examined from Co- lombia. Disposition of specimens: these Neivamyrmex remain in my collec- Vol. 109, No. 2, March & April, 1998 139 tion. They originally came from Colombia via Mr. Allen Graffham. The specific locality for this material is unknown, but Schlee (1984) indi- cated this may be from "Pena Blanca." This material bears locality informa- tion of Santander Department, Colombia. Allen Graffham (pers. comm.) indi- cated it came from a roadcut and provided several photographs. He indicated that he doubts any deposition has occurred in this area for a very long time as it is quite mountainous with very steep slopes. I have examined fossil ants in copal from both Santandar and Boyaca departments. Most material was col- lected at a depth of 2 - 3 meters in Santandar and up to 10 meters in Boyaca. Poinar (1992) indicated all known Colombian material is of Pleistocene age. Ken Anderson (pers. comm.) also considered these were "resins of undeter- mined geological age, but probably not of great antiquity." This copal is presumed to be derived from a species of Hymenaea, but details regarding this locality, deposition, and origin of this material are sketchy at best. It is not known to this author whether any species of Hymenaea live in 8 Figures 1 - 8. Scale varying. Legs, pilosity, and sculpture omitted. Figures 1 - 3. Neivamyrmex iridescens. Figure 1 . Worker, lateral view. Figure 2. Head, profile of occipital vertex. Remainder of head obscured by position within copal. Figure 3. Petiole and postpetiole, dorsal view. Figures 4 - 8. Dorylus molestus. Figure 4. Minor worker, lateral view. Figure 5. Major worker, head, full face view. Figure 6. Major worker, lateral view. Figure 7. Worker, petiole and postpetiole, dorsal view. Figure 8. Major worker, mandibles and anterior clypeal margin. 140 ENTOMOLOGICAL NEWS Figures 9-12. Photographs of fossilized army ants and driver ants. Scale varying. Figures 9-10. Neivamyrmex iridescens. Figure 9. Damaged specimen (damaged previously by polishing). Fig- ure 10. Worker, lateral view. Figures 11-12. Dorylus molestus. Figure 1 1 . Numerous workers in copal. Figure 12. Major worker, lateral view. Vol. 109, No. 2, March & April, 1998 141 the area today. Figures 1 - 3 show typical features of these specimens. Figures 9 and 10 depict the general appearance of these specimens within the copal. Other arthropods were preserved with the ants. Their state of preservation coupled with problems in identification of South American insects did not permit pre- cise identifications. Specimens include two other ants (one minor worker Pheidole sp., one worker A nochetus sp.), one Chalcidoidea, one fly, one worker termite, one set of termite wings, and one spider. Since only four ants were trapped, it is possible that they were on the periphery of a foraging column which encountered the resin. ACKNOWLEDGMENTS This paper is dedicated to the memory of the late Frank M. Carpenter (Museum of Compara- tive Zoology, Harvard Univ. advisor and friend for the past 2 decades). His encouragement was appreciated. My continuing thanks is extended to Jeri and Benjamin DuBois for their support and under- standing. They make it all possible. Lloyd Davis (Gainesville, Florida) provided some specimens of Dorylus. Julian Watkins II (Baylor University, Waco, Texas) identified some specimens of Neivamyrmex iridescent. Bill Gotwald (Utica College of Syracuse University, Utica, New York) identified some specimens of Dorylus molestus. Merrill Foster (Bradley University, Peoria, Illi- nois) put me in contact with Allen Graffham (Ardmore, Oklahoma) who provided numerous comments regarding localities and possible ages for the ant fossils discussed herein. The major- ity of fossil ants discussed herein also came from Allen Graffham. F. von Gnielinski (Geological Survey of Queensland, Brisbane, Queensland) provided insights into the age and conditions of deposition of the African copal. Thanks also to Ken Anderson (Argonne National Laboratory, Argonne, Illinois) who reviewed his current analyses of assigning ages to copal and amber world- wide and discussed his view of the age of these resins with me. This paper has been reviewed by Wallace LaBerge and Don Webb (Illinois Natural History Survey, Champaign, Illinois), Julian Watkins II, Stefan Cover (Museum of Comparative Zool- ogy, Harvard University), and three anonymous reviewers. Where possible, I have tried to incor- porate their comments. I accept responsibility for any remaining errors or omissions. LITERATURE CITED Bolton, B. 1994. Identification Guide to the Ant Genera of the World. Harvard Univ. Press, Cambridge, MA. 222 pp. Borgmeier, T. 1955. Die Wanderameisen der Neotropischen Region (Hym. Formicidae). Studia Entomol. 3: 1 -716. Carpenter, F. M. 1930. The fossil ants of North America. Bull. Mus. Comp. Zool., Harvard Univ. 70: 1 - 66. Carpenter, F. M. 1992. Part R, Arthropoda 4, Volumes 3 and 4, Superclass Hexapoda. In Kaesler, R. J., E. Brosius, J. Keim, J. Priesner (eds.). Treatise on Invertebrate Paleontology, xxii + 655 p. 1489 figs. Univ. Kansas Press, Lawrence, KS. [Formicidae, pp. 490 - 495]. DuBois, M. B. 1993. What's in a name? A clarification of Stenamma westnoodi, S. debile, and 5. lippulum (Hymenoptera: Formicidae: Myrmicinae). Sociobiology 21(3): 299 - 334. Francoeur, A. and S. A. Elias. 1985. Dolichoderus taschenbergi Mayr (Hymenoptera: Formicidae) from an early Holocene fossil insect assemblage in the Colorado Front Range. Psyche 92 (2-3): 303 - 307. 142 ENTOMOLOGICAL NEWS Larsson, S. G. 1978. Baltic amber - a palaeobiological study. Entomonograph Vol. 1 . Klampen- borg, Denmark. 192 pp. Mayr, G. 1868. Die Ameisen des baltischen Bemsteins. Beitr. Naturk. Preussens 1:1- 102. Poinar, G. O., Jr. 1992. Life in Amber. Stanford Univ. Press, Stanford, Calif, xiii + 350 pp., 8 pi. Raignier, A. and J. Van Boven. 1 955. Etude taxonomique, biologique et biometrique des Dorylus du sous-genre Anomma (Hymenoptera Formicidae). Ann. Musee Royal Congo Beige n.s. 4 (Sci. Zool.) 2: 1 - 359. Schlee, D. 1984. Notizen ubereinige Bemsteine und Kopule aus aller Weit. Stuttg. Beitr. Natkde. 18:29-38. Schluter, T. and F. von Gnielinski. 1987. The East African Copal Its geologic, stratigraphic, palaeontologic significance and comparison with fossil resins of similar age. Nat. Mus. Tan- zania Occ. Pap. 8: 1 - 34. Stockley, G. M. 1928. Report on the geology of the Zanzibar Protectorate. 126 pp. Zanzibar. Watkins, J. F., II. 1976. The identification and distribution of New World army ants (Dorylinae: Formicidae). Markham Press Fund Baylor Univ. Press, Waco, TX, x + 102 pp. Wheeler, W. M. 1914 (1915). The ants of the Baltic amber. Schriften der Physikalisch Okonomischen Gesellschaft zu Konigsberg 55: 1-142. Wheeler, W. M. 1922. Ants of the American Museum Congo Expedition, a contribution to the myrmecology of Africa, I: On the distribution of the ants of the Ethiopian and Malagasy regions; II: The ants collected by the American Museum Congo Expedition; VII: Keys to the genera and subgenera of ants; VIII: A synonymic list of the ants of the Ethiopian region; IX: A synonymic list of the ants of the Malagasy region. Bull. Amer. Mus. Nat. Hist. 45( 1 ): 13- 27, 39 - 269, 631 - 710, 71 1 - 1004 1005 - 1055. Wilson, E. O. 1985. Ants of the Dominican amber (Hymenoptera: Formicidae). 2. The first fossil army ants. Psyche 92 (1): 11 - 16. Vol. 109, No. 2, March & April, 1998 143 COCCINELLIDAE (COLEOPTERA) IN APPLE ORCHARDS OF EASTERN WEST VIRGINIA AND THE IMPACT OF INVASION BY HARMONIA AXYRIDIS1 M. W. Brown, S. S. Miller2 ABSTRACT: Twenty-five species of Coccinellidae were found on apple in eastern West Virginia from 1983 to 1996. From 1989 through 1994, the exotic coccinellid, Coccinella septempunctata (first collected in 1983), has dominated the fauna of the tribe Coccinellini. Another adventive species, Harmonia axyridis (first collected in 1994), became the dominant species in the tribe Coccinellini in 1995, and continues to dominate the coccinelline guild on apple. Harmonia axyridis has displaced C. septempunctata and is providing better biological control of Aphis spiraecola on apple in eastern West Virginia. Coccinellids (Coleoptera: Coccinellidae) are an important group of aphid predators in many ecosystems. Because of their ability to contribute to the control of pests, many coccinellids have been selected for introduction in bio- logical control programs. As of 1985, there had been 179 introductions of coccinellids into the U. S., with 26 species having become established; how- ever, 8 were a result of accidental introductions (Gordon 1985). Two recent coccinellid immigrants have made an obvious impact in the mid-Atlantic states. In 1973, Coccinella septempunctata L. was first found in New Jersey (Angalet et al. 1979), and it gradually spread throughout the Northeast (Schaefer et al. 1987). In 1988, H. axyridis was first found in Louisiana (Chapin and Brou 1991), and has since spread into the mid- Atlantic region (Day et al. 1994). Although both species had been released intentionally, their establishment ap- pears to be a result of accidental introductions (Day et al. 1994). Both species are now abundant throughout much of the northeastern United States and east- ern Canada (Hoebeke and Wheeler 1996) and have even become subjects of public concern because of occasional massive migrations of C. septempunctata (Schaefer et al. 1987) and by large numbers of H. axyridis overwintering in houses (Knodel and Hoebeke 1996). There have been numerous studies cataloguing the coccinellids in apple orchards in Europe and North America. In Europe, more than 23 species of coccinellids have been collected on apple, but usually 3 to 14 species in any one study (Hodek and Honek 1996). In a study spanning 4 years in 3 regions of Hungary, 23 coccinellid species were found on apple (Marko et al. 1 995). In Europe, Adalia bipunctata (L.) and C. septempunctata dominate in apple 1 Received July 7, 1997. Accepted September 6, 1997. 2 USDA, ARS, Appalachian Fruit Research Station, 45 Wiltshire Road, Keameysville, WV 25430. ENT. NEWS 109(2) 136-142, March & April, 1998 144 ENTOMOLOGICAL NEWS orchards. The number of coccinellid species recorded from North American orchards has been similar to that reported in Europe: 7 species in Ontario (Smith 1957), 17 in another study in Ontario (Hagley 1974), 15 in Quebec (LeRoux 1960), 8 in Washington state (Carroll and Hoyt 1984), and 6 in Pennsylvania (Horsburgh and Asquith 1 968). Putman ( 1 964) found 1 0 species of coccinellids in peach orchards in Ontario. In Washington, Coccinella transversoguttata Faldermann was the most abundant (Carroll and Hoyt 1984) but in the east, the dominant species varied temporally and spatially. In this paper, we character- ize the coccinellid fauna of apple orchards in eastern West Virginia. Data are taken from several studies covering a 14-year period, 1983 to 1996. These years span the time of the invasion of//, axyridis into the region and are used to evaluate the effect of H. axyridis on other species in the tribe Coccinellini, and on Aphis spiraecola Patch (Homoptera: Aphididae) populations. MATERIALS AND METHODS Data from several studies were used to characterize the Coccinellidae in apple orchards in eastern West Virginia. Except for the years 1993 to 1995, both conventionally managed and unsprayed orchards were sampled in each study. Data from 1983 to 1984 were taken from Brown and Adler (1989), and Brown et al. (1988). These studies covered the region from Virginia to New York, but only data from West Virginia are used in the present paper. Sampling consisted of visual examination of 7 branches from 6 trees in each of 4 or- chards, repeated 6 times per year. The purpose of the study was to examine the phytophagous community structure on apple, but occurrence, not abundance, of natural enemies was also recorded. Data from 1 984 to 1 988 were from Brown and Welker (1992) using whole-tree visual sampling from 5 to 10 trees from each of 3 orchards, 4 to 6 times per year. Data from 1989 to 1990 were from Brown (1993), sampling in 2 orchards, 2 branches from each of 10 trees in 1989 and 5 trees in 1990, 6 samples per year. Data from 1991 were taken from limb-jarring samples from 3 branches of 6 apple trees each hour over a 24- hour period 4 times during the summer. Limb-jarring samples from 2 peach trees per hour and one sour cherry tree every other hour provide data for Coccinellidae on those tree species. A second study in 1991 added data for Coccinellidae on peach trees (Brown and Puterka 1997) and used observations on 6 branches from each of 6 trees in 3 orchards at 5 times during the summer. Coccinellid data from 1992 to 1996 were based largely on two experimen- tal orchards planted in 1992: one managed with conventional methods, and the second managed with reduced insecticides and diverse ground cover plantings underneath the trees. In 1992, sampling involved two, 15-tree transects per orchard 6 times per year (Brown and Lightner 1997). Every terminal on each sample tree was examined for aphids and predators. Monthly samples from 10 randomly selected trees per orchard, 10 shoots per tree, were collected from Vol. 109, No. 2, March & April, 1998 145 1993 to 1995 (Brown etal. 1997). In 1996 the same two orchards were sampled more intensively to estimate populations of A. spiraecola and aphid predators. Ten terminals on 20 randomly selected trees were sampled weekly in May and June, then biweekly into September whenever aphids were present. In 1992 and 1996, three other orchards were also sampled with the same methodology as described above for those years. Identification of adult Scymninae, Sticholotidinae, and Psylloborini were made by R. D. Gordon, USDA, ARS, Systematic Entomology Laboratory, Washington, DC. Adult Coccinellinae (except Psylloborini) and Chilocori- nae were identified using Dillon and Dillon (1961). Data on populations of A. spiraecola, the predominant aphid on apple trees in West Virginia (Pfeiffer et al. 1989), resulted from pest monitoring in three conventionally managed apple orchards. Ten terminals on two trees from each orchard were sampled and the number of leaves per terminal infested with aphids was recorded. Sampling was conducted every week from early May to September from 1992 to 1996. Assessment of the impact of//, axyridis on coccinellids was limited to the tribe Coccinellini. Any effect on the endemic fauna would be expected to be greatest on members of the same tribe because of their generally similar biolo- gies and niche requirements. RESULTS AND DISCUSSION Twenty-five species of coccinellids in 4 subfamilies and 9 tribes were found on apple in eastern West Virginia from 1983 to 1996 (Table 1). Fourteen spe- cies of coccinellids were found on peach, including Scymnus caudalis LeConte, which was not found on apple, and 7 on sour cherry in limited sampling on these host trees (Table 1). In addition to those species listed, 4 others, all be- longing to the Coccinellini, were caught in sticky or blacklight traps located in apple orchards but were not seen foraging on fruit trees: C. transversoguttata, Hippodamia convergens Guerin, H. tredecimpunctata tibialis (Say), and Mulsantina picta (Randall). The 25 species listed in Table 1 represent a larger and more comprehensive list of the coccinellid fauna on apple than other stud- ies because it covers a 14-year period, whereas all previous studies, except for Marko et al. (1995), covered only 1 to 3 years. For the 8 years in which numerical data are available (1989 to 1996), the two exotic species, C. septempunctata and H. axyridis, dominated the coccinelline fauna (Table 2). Although 7 species of Coccinellini were recorded on apple during these years, over 70% of the individuals collected belonged to the two exotic species. Coccinella septempunctata was first collected from apple in West Virginia in 1983. By 1985, C. septempunctata was the most frequently encountered coccinelline in orchards, and it was the only coccinelline 146 ENTOMOLOGICAL NEWS Table 1. Coccinellidae, by subfamily and tribe (after Kovar 1996), found in apple orchards of eastern West Virginia, 1983-1996. Scymninae Stethorini Stethorus punctum (LeConte) 1 Scymnillini (Zilini) Zilus horni Gordon Scymnini Diomus terminates (Say) 1 ,2 Scymnus fraternus LeConte S. circumspectus Horn 2 5. iowensis Casey 1 ,2 S. rubricaudus Casey Scymnus sp. 1 Brachiacanthini Brachiacantha ursina (F.) Hyperaspidini Hyperaspis proba (Say) 1,2 Chilocorinae Chilocorini Chilocorus stigma (Say) 1,2 Coccinellinae Coccinellini Adalia bipunctata (L.) ' Anatis labiculata (Say) Anatis mali (Say) 1 Coccinella novemnotata Herbst C. septempunctata L. 1 ,2 C. transversoguttata Faldermann Coleomegilla maculata lengi Timberlake 1 Cycloneda munda (Say) 1 C. sanguinea (L.) Harmonia axyridis (Pallas) Hippodamia parenthesis (Say) Olla v-nigrum (Mulsant) 1 Psylloborini Psyllobora vigintimaculata (Say) 2 Sticholotidinae Microweiseini Microweisea misella (LeConte) 1 Also found on peach trees; Scymnus caudalis LeConte (Scymninae: Scymnini) was found only on peach trees. Also found on sour cherry trees. Table 2. Percentage composition of the coccinelline fauna on apple in eastern West Virginia, 1989-1996. Species 1989-90 1991 1992 1993 1994 1995 1996 C. septempunctata 72.7 86.4 99.5 93.2 79.4 9.1 10.2 A. bipunctata 9.1 2.3 1.7 C. munda 9.1 4.5 0.5 A. labiculata 9.1 C. maculata lengi 2.3 5.1 2.9 27.3 1.1 O. v-nigrum 4.5 H. axyridis 17.7 63.6 88.7 Number observed 11 44 192 59 34 11 265 Vol. 109, No. 2, March & April, 1998 147 seen in 1987 and 1988 during sampling of the same orchards (Kozar et al. 1994). Coccinella novemnotata Herbst, which Wheeler and Hoebeke (1995) suggest may be negatively correlated with C. septempunctata, has not been seen in West Virginia apple orchards since 1985. In a survey prior to the arrival of C. septempunctata in nearby south-central Pennsylvania (Horsburgh and Asquith 1968), the only Coccinellini found were A. bipunctata, C. munda, and H. convergens. Harmonia axyridis, first collected in 1994, has replaced C. septempunctata as the dominant coccinelline in apple orchards (Fig. 1). In 1995, C. septempunctata was reduced to the third most abundant species of Coccinellini behind H. axyridis and Coleomegilla maculata lengi Timberlake (Table 2). Of 1 00% 90 C. septumpunctata _J Harmonia axyridis LJ All others Fig. 1. Percentage composition of the coccinelline fauna in apple orchards of eastern '.Vest Vir- ginia from 1990 to 1996, showing the displacement of C. septempunctata by H. axyridis. 148 ENTOMOLOGICAL NEWS the less abundant coccinellids, A. bipunctata, Cycloneda munda (Say), Anatis labiculata (Say), and Olla v-nigrum (Mulsant) have not been seen in apple orchards in the three years since H. axyridis appeared. Only C. maculata lengi has seemed to become more abundant on apple since the arrival of//, axyridis. Harmonia axyridis has shown its ability to rapidly dominate in other ecosys- tems in other regions where it has invaded (Tedders and Schaefer 1994, Day et al. 1994). Comparing the two years in which intensive sampling of aphid predators was conducted, 1992 and 1996, gives insight into the interaction between C. septempunctata and H. axyridis (Table 3). In 1992, all but one coccinelline Table 3. Number of Coccinellini collected in two intensive surveys of aphid predators in eastern West Virginia apple orchards. 1992 1996 Species Adults Immatures Adults Immatures C. septempunctata 92 99 27 0 C. munda 1 0 0 0 H. axyridis 0 0 48 187 C. maculata lengi 0 0 3 0 Unidentified 0 0 0 12 was C. septempunctata, with about equal numbers of adults and immatures. By 1996, two years after H. axyridis was first detected, no C. septempunctata immatures were seen on apple trees. The 12 unidentified immatures were either egg masses or first instar larvae that we could not identify to species. The reduction in total numbers of C. septempunctata from 1992 to 1996 sug- gests that //. axyridis not only replaced C. septempunctata as the dominant species but also largely displaced it from apple, especially in its use of apple as a larval habitat. It has been shown in Japan that H. axyridis larvae prey on C. septempunctata brucki Mulsant, but predation in the reverse direction does not occur (Hironori and Katsuhiro 1997). The displacement of C. septempunctata by H. axyridis has had a positive impact on biological control of A. spiraecola in West Virginia apple orchards (Fig. 2.). Aphid populations were much lower from 1994 to 1996 than in pre- vious years; peak aphid populations were lower and the duration of aphid in- festations was shorter, particularly in 1996 (Fig. 2). The aphid data were from orchards sprayed with insecticides, explaining the sharp declines in popula- tion abundance in most years. Harmonia axyridis was first found in apple or- chards in 1994, but it did not dominate until 1995, when aphid populations appeared to be suppressed. Only in 1995 and 1996, the two years in which //. Vol. 109, No. 2, March & April, 1998 149 ro c 0) S 0 I o 120 140 160 1991 180 Day 1992 200 220 240 1993 CD C E I I o I 240 1996 Fig. 2. Population estimates of A. spiraecola by day of the year in three conventionally managed orchards in eastern West Virginia; A, 1991 to 1993, prior to arrival of H. axyridis; B, 1994-1996, after arrival of//, axyridis. 150 ENTOMOLOGICAL NEWS axyridis dominated the coccinelline fauna, were insecticide sprays not applied to control aphids. Data on aphids were obtained only from a few trees in three orchards in only one location and, therefore, cannot be considered conclusive. The indication, however, is that the arrival of H. axyridis has improved the biological control of aphids on apple. Conclusions: Exotic species of Coccinellini have greatly affected the coccinelline fauna of West Virginia apple orchards. First, C. septempunctata dominated the fauna, but it was in turn displaced by the newly arrived H. axyridis in 1995. The overall effect of H. axyridis on native Coccinellini has not been evaluated, but since the arrival of C. septempunctata, native coccinellines have represented only a very minor component of the fauna on apple. Aphid bio- logical control has been enhanced since the arrival of//, axyridis, so this latest addition to the coccinelline fauna has been beneficial in the apple agroeco- system. ACKNOWLEDGMENTS We thank R. D. Gordon (USDA — ARS, Systematic Entomology Laboratory, Beltsville, MD) for identifications of the Scymninae, Sticholotidinae, and Psylloborini; L. Claire Stuart, Cynthia R. L. Adler, Donald C. Weber, Jeffrey J. Schmitt, and V. Larry Crim for their data collection; and William H. Day, Henry W. Hogmire, Jeffrey J. Schmitt, and Natalia J. Vandenberg for their com- ments on an earlier draft of this paper. LITERATURE CITED Angalet, G. W., J. M. Tropp, and A. N. Eggert. 1979. Coccinella septempunctata in the United States: recolonizations and notes on its ecology. Environ. Entomol. 8: 896-901. Brown, M. W. 1993. Resilience of the natural arthropod community on apple to external distur- bance. Ecol. Entomol. 18: 169-183. Brown, M. W. and C. R. L. Adler. 1989. Community structure of phytophagous arthropods on apple. Environ. Entomol. 18: 600-607. Brown, M. W., C. R. L. Adler, and R. W. Weires. 1988. Insects associated with apple in the mid-Atlantic states. New York's Food and Life Sci. Bull. No. 124, 1-31. Brown, M. \V., D. M. Glenn, and T. van der Zwet. 1997. Impact of ground cover plants on pest management in West Virginia, USA, apple orchards. Hortic. Sci. (Prague) 24:39-44. Brown, M. W. and G. W. Lightner. 1997. Recommendations on minimum experimental plot size and succession of aphidophaga in West Virginia, USA, apple orchards. Entomophaga. 42: 259-269. Brown, M. W. and G. J. Puterka. 1997. Orchard management effects on the arthropod commu- nity on peach with comparisons to apple. J. Entomol. Sci. 32: 165-182. Brown, M. W. and W. V. Welker. 1992. Development of the phytophagous arthropod commu- nity on apple as affected by orchard management. Environ. Entomol. 21: 485-492. Carroll, D. P. and S. C. Hoyt. 1984. Natural enemies and their effects on apple aphid, Aphis pomi DeGeer (Homoptera: Aphididae), colonies on young apple trees in central Washington. Environ. Entomol. 13: 469-481. Chapin, J. B. and V. A. Brou. 1 99 1 . Harmonia axyridis (Pallas), the third species of the genus to be found in the U.S. (Coleoptera: Coccinellidae). Proc. Entomol. Soc. Wash. 93: 630-635. Vol. 109, No. 2, March & April, 1998 151 Day, W. H., D. R. Prokrym, D. R. Ellis, and R. J. Chianese. 1994. The known distribution of the predator Propylea quatuordecimpunctata (Coleoptera: Coccinellidae) in the United States, and thoughts on the origin of this species and five other exotic lady beetles in eastern North America. Entomol. News 105: 244-256. Dillon, E. S. and L. S. Dillon. 1961. A manual of common beetles of eastern North America. Row, Peterson & Company. Evanston, 111. Gordon, R. D. 1985. The Coccinellidae (Coleoptera) of America north of Mexico. J. New York Entomol. Soc. 93: 1-912. Hagley, E. A. C. 1974. The arthropod fauna in unsprayed apple orchards in Ontario. II. Some predacious species. Proc. Entomol. Soc. Ont. 105: 28-40. Hironori, Y. and S. Katsuhiro. 1997. Cannabalism and interspecific predation in two ladybirds in relation to prey abundance in the field. Entomophaga. 42: 153-163. Hoebeke, E. R. and A. G. Wheeler. 1 996. Adventive lady beetles (Coleoptera: Coccinellidae) in the Canadian maritime provinces, with new eastern U.S. records. Entomol. News 107: 281- 290. Hodek, I. and A. Honek. 1996. Ecology of Coccinellidae. Kluwer Academic Publishers. Bos- ton. Horsburgh, R. L. and D. Asquith. 1968. Initial survey of arthropod predators of the European red mite in south-central Pennsylvania. J. Econ. Entomol. 61: 1753-1754. Knodel, J. J. and E. R. Hoebeke. 1996. Multicolored Asian lady beetle, Harmonia axyridis (Pallas) Coleoptera: Coccinellidae. Cornell Coop. Ext., Misc. Pest Management Fact Sheet, page 101.00. Kovar, I. 1996. Phylogeny. In: Hodek, I. and A. Honek. Ecology of Coccinellidae. Kluwer Aca- demic Publishers. Boston, pp: 19-31. Kozar, F., M. W. Brown and G. Lightner. 1994. Spatial distribution of homopteran pests and beneficial insects in an orchard and its connection with ecological plant protection. J. Appl. Entomol. 117:519-529. LeRoux, E. J. 1960. Effects of "modified" and "commercial" spray programs on the fauna of apple orchards in Quebec. Ann. Soc. Entomol. Quebec 6: 87-121. Mark6, V., O. Merkl, A. Podlussany, K. Vig, Cs. Kutasi, and S. Bogya. 1995. Species compo- sition of Coleoptera assemblages in the canopies of Hungarian apple and pear orchards. Acta Phytopathol. Entomol. Hung. 30: 221-245. Pfeiffer, D. G., M. W. Brown, and M. W. Yarn. 1989. Incidence of spirea aphid (Homoptera: Aphididae) in apple orchards of Virginia, West Virginia, and Maryland. J. Entomol. Sci. 24: 145-149. Putman, W. L. 1964. Occurrence and food of some coccinellids (Coleoptera) in Ontario peach orchards. Can. Entomol. 89: 1 149-1 155. Schaefer, P. W., R. J. Dysart, and H. B. Specht. 1 987. North American distribution of Coccinella septempunctata (Coleoptera: Coccinellidae) and its mass appearance in coastal Delaware. Environ. Entomol. 16: 368-373. Smith, B. C. 1957. Notes on relative abundance and variation in elytral patterns of some com- mon coccinellids in the Belleville district (Col.: Coccinellidae). Rep. Entomol. Soc. Ont. 88: 59-60. Tedders, W. L. and P. W. Schaefer. 1994. Release and establishment of Harmonia axyridis (Coleoptera: Coccinellidae) in the southeastern United States. Entomol. News 105: 228-243. Wheeler, A. G., Jr. and E. R. Hoebeke. 1995. Coccinella novemnotata in northeastern North America: Historical occurrence and current status (Coleoptera: Coccinellidae). Proc. Entomol. Soc. Wash. 97: 701-716. 152 ENTOMOLOGICAL NEWS SOCIETY MEETING OF OCTOBER 22, 1997 Dr. Susan P. Whitney University of Delaware, Cooperative Extension SUBTERRANEAN TERMITE COMMUNITY ECOLOGY Dr. Whitney began by explaining that her research is an effort to answer basic questions about termite ecology, sparked by the need to find new controls. Chlordane, the old standby, has been unavailable since 1 988. Subterranean termites are the number two household insect in terms of damage caused. After providing a brief account of the natural history of termites, Dr. Whitney reported on studies of three species of subterranean termites in two sites in Delaware. Reticulitermesflavipes, the eastern subterranean termite, is found in wooded areas in northern Delaware. R. virginicus and R. hageni are found in dry habitats in southern Delaware. During the summers (May-Sep- tember) of 95 and 96, field sites were monitored for termite activity with pine stakes buried approximately 20 cm in the ground. Stakes that showed feeding were replaced with "bucket traps" — plastic buckets with the bottom cut off. Into each bucket was placed a wood "sandwich" — six pieces of pine arranged to allow gaps for workers to build mud tubes. Termites were removed from an initial bucket at the start of the field season. Individual workers were marked by allowing them to feed on filter paper saturated with Nile blue A dye. After three days of feeding, marked workers were returned to their bucket. After one week all wood "sandwiches" in the field site were examined for termite presence. The data from repeated marking and recapture were analyzed and average number of workers in colonies of each species calculated. Foraging dis- tances were determined to be as much as thirty feet. Comparisons of the two sites, the experi- mental farm at University of Delaware in Newark and the field station at Lewes, suggested that colony size and relations among the three species are different in the dry pinewoods at Lewes. Dr. Whitney described her plans to confirm these findings at other sites and to try reducing colonies with toxic baits to test population recovery. In entomological notes, Susan Whitney reported finding mole crickets in Delaware; Jon Gelhaus noted AES treasurer Howard Boyd's new book on the New Jersey Pine Barrens; and Roger Fuester reported on the low levels of gypsy moth in Delaware this year - no acreage over 30% defoliated. W. J. Cromartie Corresponding Secretary NOTE RE PRIORITY OF NEW SPECIES DESCRIBED IN JANUARY-FEBRUARY 1998 ISSUE OF ENTOMOLOGICAL NEWS The January-February 1998 issue, Vol. 109, No. 1, of Entomological News was mailed on December 1, 1997. Due to this early mailing date, and for purposes of priority, any new species described in that issue must be cited with a 1997 date, even though it appeared in a 1998 issue. H.P.B., ed. When submitting papers, all authors are requested to (1) provide the names of two qualified individuals who have critically reviewed the manuscript before it is submitted and (2) suggest the names and addresses of two qualified authorities in the subject field to whom the manuscript may be referred by the editor for final review. All papers are submitted to recognized authorities for final review before acceptance. Titles should be carefully composed to reflect the true contents of the article, and be kept as brief as possible. 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VOL. 109 QL- MAY & JUNE, 1998 US ISSN 0013-872X NO. 3 ENTOMOLOGICAL NEWS (Plecoptera: Perlidae), a new stonefly species from Alabama A, D. Smith, B. P. Stark 153 Biology & hosts of Procecidochares atra (Diptera: Tephritidae): evidence for cryptic species T.K. Phillips, D.C. Smith 159 Survey of summer tiger beetles on Ohio River beaches in Ohio & eastern Indiana G. Kritsky, AJ. Savage, S. Reidel, J. Smith 165 Ticks of genus Amblyomma (Acari: Ixodidae) from white- lipped peccaries in northeastern Bolivia, with comments on host specificity R.G. Robbins, W.B. Karesh, R.L.E. Painter, S. Rosenberg 172 New records of Japygoidea (Diplura) from Louisiana, with notes on behavior MA. Muegg, C.E. Carlton 111 New records ofAllocapnia (Plecoptera: Capniidae) from Mississippi & Louisiana, with scanning electron micrographs Mac H. Alford 183 First U.S. record ofDyschirius sextoni (Coleoptera: Carabidae) F.F. Purrington, J.A. Maxwell 189 Two new species of Hynesionella (Heteroptera: Gerridae) from South Africa J.T. Polhemus 191 Reassignment of Isotoma louisiana (Collembola: Isotomidae R.D. Waltz 195 New host record for Sphaeropthalma pensylvanica pensyl- vanica (Hymenoptera: Mutellidae) D.G.Manley,T.P.Carithers 198 Odonata of south central Nearctic Region, including northeastern Mexico J.C. Abbott, E.W. Stewart 201 SCIENTIFIC NOTES: New distributions for Raptoheptagenia cruentata & Ametropus neavei (Ephemeroptera) R.D. Waltz, G.F. Edmunds, Jr., G. Lester 213 Cloeodes excogitatus (Ephemeroptera: Baetidae) in northern California R.D. Waltz, Peter Ode, Jon Lee 215 First record ofAleiodes depanochora (Hymenoptera: Braconidae) from Brazil A.M. Penteado-Dias 217 BOOK REVIEW 164 MEMBERSHIP LIST-AMERICAN ENTOMOLOGICAL SOCIETY 219 SOCIETY MEETING OF NOVEMBER 19, 1997 224 ANNOUNCEMENT 200 THE AMERICAN ENTOMOLOGICAL SOCIETY ENTOMOLOGICAL NEWS is published bi-monthly except July-August by The American Entomological Society at the Academy of Natural Sciences, 1900 Benjamin Franklin Parkway, Philadelphia, Pa., 19103-1195, U.S.A. The American Entomological Society holds regular membership meetings on the fourth Wednesday in October, November, February, March, and April. The November, February and April meetings are held at the Academy of Natural Sciences in Philadelphia, Pa. The October and March meetings are held at the Department of Entomology, University of Delaware, Newark, Delaware. Society Members who reside outside the local eastern Pennsylvania, southern New Jersey, and Delaware area are urged to attend society meetings whenever they may be in the vicinity. Guests always are cordially invited and welcomed. 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SECOND CLASS POSTAGE PAID AT VINCENTOWN, NEW JERSEY, 08088, U.S.A. Vol. 109, No. 3, May & June, 1998 153 NEOPERLA COOSA (PLECOPTERA: PERLIDAE), A NEW STONEFLY SPECIES FROM ALABAMA1 Angela D. Smith, Bill P. Stark2 ABSTRACT: Neoperla coosa, a new stonefly species, is described from male, female and egg stages. The species resembles Neoperla osage from the Ozark Mountains, but the male aedeagus is more similar to that of Neoperla clymene. The new species is known only from the Coosa and Cahaba drainage systems of Alabama. During a scanning electron microscopy study of chorionic variability among Alabama and Mississippi Neoperla, several populations from the Coosa and Cahaba systems with distinctive eggs were found. These eggs were quite un- like those of known Neoperla from the southeastern United States (Stark and Baumann 1978; Stark and Lentz 1988; Stark 1995) and subsequently the fe- males were found to be associated at several localities with males misidentified as N. clymene (Newman). Because the eggs closely resemble those of N. osage Stark and Lentz, comparisons were made of the male and female genitalia of these species. Results of this study suggest the Coosa-Cahaba specimens rep- resent a previously unrecognized species of the N. clymene complex. Termi- nology for the description follows Stark and Lentz (1988) and Stark (1995). The holotype is deposited in the United States National Museum of Natural History (USNM) and paratypes are deposited in the University of Alabama (UA) or in the collection of the junior author (BPS). Neoperla coosa, NEW SPECIES Male.- Forewing length 9-11 mm. General color pale brown, head pale except for dark ocellar and mesal clypeal areas. Wing membrane and veins brown. Legs brown, cerci pale. Pro- cess of tergum 7 apically truncate, upturned in lateral aspect, armed ventrally with prominent sensilla basiconica. Mesal sclerite of tergum 8 triangular. Hemiterga rounded apically; finger- like process of hemiterga slender and relatively straight (Fig. 1 ). Tube of aedeagus slightly sinu- ate, ca. four times as long as bulb width (Fig. 2); spicule patch relatively prominent along dorsobasal to dorsomesal sides and margins of tube (Figs. 2, 10). Sac unarmed in basal third, apical sac armature of scattered, large and small spines (Fig. 2). Apex of tube curved slightly ventrad (Fig. 2). Female.- Forewing length 11-13 mm. Color pattern similar to male. Posterior margin of sternum 8 sinuate, mesal portion slightly produced to approximate level of adjacent lateral areas (Fig. 3). Spermathecal stalk robust and irregularly armed with fine brown setae; apex of arma- ture truncate with subapical basally directed extensions (Fig. 4). Egg.- Length ca. 331.0 ± 7.0 |im; equatorial width ca. 181.1 ±3.9 (im. Collar sessile. Received August 22, 1997. Accepted September 29, 1997. Biology Department, Mississippi College, Clinton, MS 39058. ENT. NEWS 109(3) 153-158, May & June, 1998 2 ? 1998 1 54 ENTOMOLOGICAL NEWS surrounded by two irregular rows of reticulation; collar diameter ca. 66.7 ± 1.4 jam (Fig. 7). Striae slender near poles, widening at equator to ca. 12.9 ± 0.6 u.m (Fig. 5). Number of visible striae from anterior aspect ca. 38. Most striae connect directly to posterior follicle cell impres- sion walls (FCIs), some arise from stalks of FCIs (Fig. 8). Sulci punctate with 2-3 rows of aeropyles (Fig. 6); equatorial width of sulci ca. 1.3 ± 0.2 urn. Micropyles form an irregular row displaced slightly from equator toward posterior pole; micropylar orifices simple, without raised lip. Poste- rior pole covered with FCIs containing ca. 21.8 ± 2.5 aeropyles. FCI walls smooth and slightly thinner than adjacent striae (Figs. 5, 8). 'types.- Holotype Cf and 41 9 paratypes from Yellowleaf Creek, Jumbo, Chilton County, Alabama, 5 July 1989, P. O'Neil, S. McGregor (Holotype and 1 9 paratype deposited at the National Museum of Natural History). Additional paratypes, all from Alabama: Cherokee Co. Spring Creek, Hwy 87, 27 June 1989, S. Harris, S. McGregor, 2 9 (UA). Chilton Co. Yellowleaf Creek, 2.5 mi SE Mineral Springs, 6 June 1989, 4 9 (UA). Walnut Creek, N Refuge Church, 5 July 1989, P. O'Neil, S. McGregor, 1 Cf, 17 9 (BPS). Clay Co. Cheaha Creek, abv. Lake Chinnabbee, 3 June 1978, B. Stark, K. W. Stewart, 1 Cf (BPS). DeKalb Co. Little River at Bear Creek, 22 June 1987, 5 C?, 98 9 (BPS). Elmore Co. Fischer Creek, 3.5 mi SW Weako, 24 June 1 987, S.Harris, P. O'Neil, 2 Cf, 3 9 (UA). Jefferson Co. Cahaba River, Trussville, 24 May 1981, S. Harris, P. O'Neil 3 9 (UA). Cahaba River, 1-59, 14 August 1984, S. Harris, P. O'Neil, 1 Cf, 1 9 (UA). Shelly Co. Camp Branch Creek, Hwy 42, 17 June 1984, S. Harris 2 Cf , 5 9 BPS). Etymology.- The species name is based on the Coosa River. Diagnosis.- The aedeagal tube of male N. coosa is generally similar to that of the group of species in which an abrupt bend at tube midlength is lacking. In the southeastern United States, N. clymene (Newman), N. coxi Stark, N. harrisi Stark and Lentz, N. occipitalis (Pictet), and TV. steward Stark and Baumann are included, but only N. clymene and N. steward, of this group, are known to occur with N. coosa. In the most recently available species key (Stark and Lentz 1988), male N. coosa are identified as N. clymene but differences in the tube dimensions and armature permit separation of these species. The follow- ing modification of "couplet 8" from the Stark and Lentz (1988) key is of- fered: 8. Tube apex essentially straight along ventral margin N. occipitalis Tube apex curved ventrad (Fig. 2) 9 9. Dorsobasal margin of tube weakly armed with spicules (Fig. 1 2); tube length ca. 6 times width at bulb N. clymene Dorsobasal margin of tube with prominent spicules (Fig. 10); tube length ca. 4 times width at bulb (Fig. 9) N. coosa Vol. 109, No. 3, May & June, 1998 155 V »\'-Y 1' -I ' "' '. I: •''//• -I Hf* sri'i1 '•''•'•''(• (• i I • t 'i *' '" i ' • I- 'r- •••MB vi& fv';v ' •\'i'//!^yf 1^ 3 ""' '° Figs. 1-4. Neoperla coosa, male and female genitalia. 1. Male terminalia, dorsal. 2. Aedeagal tube, sac partially everted, lateral, ventral side directed to the left. 3. Female stema 7-9. 4. Vagina and spermathecal stalk, dorsal. B - bulb; T - tube; S - sac; T7 - tergum 7; T8 - tergum 8; S8 - sternum 8; Ss - spermathecal stalk; Sp - spicule patch; Ht - hemitergum; Htp - hemitergal process. 156 ENTOMOLOGICAL NEWS 8 Figs. 5-8. SEM micrographs of Neoperla coosa eggs. 5. Egg, lateral. 6. Detail of striae. 7. Detail of collar and anchor. 8. Detail of posterior pole with FCIs. C = collar; FCI = follicle cell impres- sion; St = stria. Vol. 109, No. 3, May & June, 1998 157 Figs. 9-12. SEM micrographs of Neope rla aedeagal tubes. 9. N. coosa. lateral (B = bulb; T = tube). 1 0. N. coosa, detail of dorsolateral spicule patch. 1 1 . N. osage, detail of dorsolateral spi- cule patch. 12. N. clymene, detail of dorsolateral spicule patch. 158 ENTOMOLOGICAL NEWS The egg (Figs. 5-8) and female subgenital plate morphology (Fig. 3) sug- gest N. coosa is most closely related to an Ozark Mountain species, N. osage Stark and Lentz. However, females of these species can usually be distinguished by examination of the spermathecal stalk lining. In N. coosa the lining re- sembles that of N. robisoni Poulton and Stewart (Ernst et al. 1986) in display- ing 2-3 irregular dark longitudinal folds (Fig. 4) but in N. osage, the lining is more uniformly distributed over the apical third of the stalk (Stark and Lentz 1988). The aedeagal tube spicule armature is also similar for N. coosa and N. osage (Figs. 10, 11) however, the tube dimensions differ for these species. Neoperla osage has the aedeagal tube length about three times the bulb width (Stark and Lentz 1988) whereas in N. coosa the tube is about four times the bulb width. Neoperla coosa is presently known from eight sites in the Coosa-Little River basin and two sites in the upper Cahaba River basin. All sites are above the Fall Line in the Piedmont Upland or Alabama Valley and Ridge Physi- ographic Section (Harris et al. 1991). ACKNOWLEDGMENTS We thank S. C. Harris for providing specimens used in this study and R. W. Baumann and B. C. Kondratieff for prepublication reviews. This study was supported in part by the Howard Hughes Medical Institute, Undergraduate Biological Sciences Education Program Grant #71 195-538901 . LITERATURE CITED Ernst, M. R., B. C. Poulton and K. W. Stewart. 1986. Neoperla (Plecoptera: Perlidae) of the Ozark and Ouachi.a Mountain region, and two new species of Neoperla. Ann. Entomol. Soc. Amer. 79:645-661. Harris, S. C., P. E. O'Neil and P. K. Lago. 1991. Caddisflies of Alabama. Geol. Surv. Ala. 142:1-442. Stark, B. P. 1995. A new species of Neoperla (Insecta: Plecoptera: Perlidae) from Mississippi. Proc. Biol. Soc. Wash. 108:45-49. Stark, B. P. and R. W. Baumann. 1 978. New species of nearctic Neoperla (Plecoptera: Perlidae), with notes on the genus. Great Basin Nat. 38:97-1 14. Stark, B. P. and D. L. Lentz. 1988. New species of nearctic Neoperla (Plecoptera: Perlidae). Ann. Entomol. Soc. Amer. 81:371-376. Vol. 1 09, No. 3, May & June, 1 998 1 59 THE BIOLOGY AND HOSTS OF PROCECIDOCHARES ATRA (DIPTERA: TEPHRITIDAE): EVIDENCE FOR CRYPTIC SPECIES?1 T. Keith Philips2, D. Courtney Smith3 ABSTRACT: Galls of Proceci dochare s atra on Solidago in late spring contained an average of 15.3 ± 5.1 gregarious larvae (n = 3). Previous records note only monothalamous galls for this species. Twenty-one larvae were found in one gall whereas the previous maximum number of larvae per gall in the genus was 13. Our spring record indicates that P. atra is bivoltine, uncom- mon for a temperate species of fruit fly. We also note new records of Solidago canadensis and Erigeron canadensis as hosts and a Eurytoma species (Hymenoptera: Eurytomidae) as a parasi- toid of P. atra. The possibility that our collections represent undescribed cryptic species of Procecidochares closely related to P. atra is discussed. Most species of Procecidochares Hendel cause galls on composite plants. Galls form either in the stems or in the flowers of hosts (Benjamin 1934). One known exception is the non-galling P.flavipes Aldrich, which reproduces in flower heads (Goeden et al. 1994). Eleven species have been described in this genus in North America north of Mexico (Foote et al. 1993). Procecidochares atra (Loew) is one of the largest and most commonly collected species in the eastern United States and ranges from Florida to Nova Scotia and West to Utah and Idaho. Only monothalamous galls (i.e., galls which contain single larvae) in late summer are known in this species (Felt 1918, Phillips 1946, Philips and Smith, unpublished). Published hosts include several species of Solidago Linneaus and an undetermined species of Aster Linneaus (Wasbauer 1972) with the latter record questionable (Foote et al. 1993). Here, we report the occurrence of galls where the larvae develop gregariously within a single gall; our observations represent the largest number of larvae per gall reported in the genus. We also discuss the probability of a bivoltine life cycle and note two new hosts for, and a parasitoid of, P. atra. METHODS Galls were collected in Franklin County, Ohio. Two galls on an undeter- mined Solidago species were collected on 8 May, 1 99 1 . One of these galls was dissected. A third gall on Solidago canadensis Linneaus was collected two years later on the 1 4 May, 1 993. A single gall growing on Erigeron canadensis Linneaus was found in August 1 992. All larvae or pupae were allowed to com- Department of Entomology, Museum of Biological Diversity, Ohio State University, 1315 Kinnear Road, Columbus, Ohio 43212. 1 Received July 30, 1997. Accepted October 5, 1997. 2 Department of Entomology, Museum of Biologic* Kinnear Road, Columbus, Ohio 43212. 3 Department of Entomology, Ohio State University, 1735 Neil Avenue, Columbus, Ohio 43210. ENT. NEWS 109(3) 159-164, May & June, 1998 1 60 ENTOMOLOGICAL NEWS plete development to adult stage in the laboratory. Twelve galls on Solidago canadensis were collected in early September, 1996. These galls were dis- sected and number of puparia per gall counted. Pupae were also examined for evidence of parasitism. The single parasitoid discovered was allowed to emerge and then killed for identification. Voucher specimens from both plant hosts, the parasitoid, and Solidago galls from one of the May collections and the September collection are deposited in the Ohio State University Insect Collec- tion and the United States National Museum. Adults which emerged from Sol- idago are also in the authors' collections. RESULTS The three late spring Solidago galls were found two to four inches above the surface of the ground at the apical meristem. The first two galls collected in 1991 contained 14 and 21 pupae. Adults emerged from five to 1 1 days after collection (13-19 May) with the majority (25 of 33 flies) emerging from the 14-16 May. Two flies failed to eclose. The third gall collected in 1993 con- tained 1 1 pupae. All adults successfully eclosed (but dates of emergence were not recorded). This third gall (in a dry state) measured 25-30 mm in length by 9 mm in width. The average number of larvae per spring Solidago gall was 15.3 ±5.1 (n = 3). Larvae develop gregariously in each gall, without separate compartments for each individual. Galls on Solidago canadensis collected in September were all mono- thalamous. Each contained a single, empty puparium except for two of them. One produced a parasitoid in the genus Eurytoma Illiger (Hymenoptera: Eurytomidae) and the other held a partially eclosed, dead P. atra. All late sum- mer Solidago galls were situated approximately midway or higher on the plant stalk on apical meristems. Both the late spring and late summer Solidago galls were composed of swollen tissue surrounded by leaves in a typical rosette growth form. A single gall collected on Erigeron canadensis in August contained only one larva which eclosed in late August or early September. The Erigeron gall was a swollen stem approximately two inches above the ground. DISCUSSION Several species of Procecidochares produce galls containing single larvae. Examples include P. atra (Felt 1918, Phillips 1 946), P. minuta (Snow) (Novak et al. 1967), P. stonei Blanc and Foote (Tauber and Tauber 1968) and various species in the literature without precise identities (Silverman and Goeden 1980, Wangberg 1 980). In other species, one to several larvae develop within a single gall. Wangberg ( 1 980) usually found one to three larvae but recorded up to six in his Procecidochares sp. "B" and from one to seven (recorded in table 1 ) for Procecidochares sp. "C." Stegmaier (1968) found P. australis Aldrich galls Vol. 109, No. 3, May & June, 1998 161 contained two to eight larvae per gall. Similarly, Phillips (1946) found this species to have up to eight, but most often had only a single larva per gall. Bess and Haramoto (1958) record an average of three larvae in each gall of P. utilis Stone. The highest reported number of larvae in a single gall is 13 in P. stonei (Green et al. 1993). But the average for this species was only 2.5 ± 0.1 larvae per gall. By comparison, in spring galls we found up to 21 larvae of P. atra in a single gall and an average of 15.3 ±5.1 (n = 3) larvae or pupae per gall. Published biologies of P. atra are not explicit on the number of larvae per gall or the life cycle. For example, Felt (1918) lists a small rosette gall contain- ing a single larval cell on Solidago altissima and refers to two species, P. polita (Loew) and P. atra (as Oedaspis polita and O. atra). He cites Stebbins (1910), but she only mentions P. polita and not P. atra. Felt (1918) either had his own records or may have listed both species because they are sympatric in Massa- chusetts, where Stebbins (1910) based her study. Phillips (1946) studied two larvae of P. atra taken from goldenrod galls, implying a single larva per gall. Galls we collected on Solidago canadensis in late summer contained only single puparia but, as previously mentioned, galls collected in late spring produced an average of 15.3 ± 5.1 (n = 3) larvae or pupae. The number of larvae per gall can vary within a species depending upon the host. Procecidochares stonei, when living in Virguiera laciniata Gray, pro- duces up to 1 3 larvae per gall (Green et al. 1 993). When this same species uses Virguiera deltoidea Gray var. parishii (Greene) the maximum number of lar- vae per gall drops to three. For unknown reasons, the number of larvae varies seasonally even within the same host in P. atra. The spring generation on Sol- idago develops with large numbers of larvae per gall, whereas the fall genera- tion, whether on Solidago or on Erigeron canadensis, occurs as a single larva per gall. Galls with more than one larva can have two different types of internal gall structure. Polythalamous galls contain separate compartments for each larva while others are without internal divisions. Species of Procecidochares are known to form both types. Wangberg's (1980) Procecidochares sp. "C" forms polythalamous galls. In contrast, P. stonei larvae develop gregariously within a gall (Green et al. 1 993). Our dissections showed that P. atra develops in galls gregariously in the spring, without separate compartments for each larva. Most species of Procecidochares appear to have a limited number of hosts (Foote et al. 1993). For those species with large numbers of reported hosts, such as P. minuta (Snow), it appears as though sibling or cryptic species are involved (Wangberg 1980, Foote et al. 1993). Previously recorded hosts for P. atra are Solidago altissima Linneaus, S. nemoralis Ait., S. odora Ait. and one questionable record from Aster (Wasbauer 1972, Foote et al. 1993). Our col- lections add two additional hosts, Solidago canadensis and Erigeron canadensis. Procecidochares anthracina (Doane) is the only species of this genus previ- 162 ENTOMOLOGICAL NEWS ously recorded from a species of Erigeron. Like P. atra, this species has been recorded from both Solidago and Erigeron. This is evidence that galling by one tephritid species on both of these hosts may be common. The other no- table aspect of our Erigeron record is that the gall was located on the stem near the ground and not on an apical meristem as in Solidago. In light of the differ- ences in both host plant and gall location, we initially thought the Erigeron galler represented a different species. But we are unable to differentiate this specimen morphologically from other specimens of P. atra. Procecidochares atra adult emergence from galls in late spring and late summer indicate a bivoltine life cycle, whereas most temperate species of tephritids have only one generation per year (Bateman 1972, Christenson and Foote 1960). There are other species of Procecidochares that are bivoltine or even multivoltine. Procecidochares utilis, a species native to Mexico, has two generations per year (Hoy 1960). The Procecidochares sp. of Silverman and Goeden (1980) is bivoltine in Southern California, although it is sometimes univoltine and conceivably even biennial if adequate rainfall, which triggers necessary vegetative regrowth, does not occur. Huettel and Bush (1972) men- tion both P. australis and an undescribed Procecidochares species as multi- voltine. These two species emerge in the fall from galls in flower heads. The adults then oviposit in small, overwintering rosette plants. Larvae and their galls develop slowly over the winter and the spring generation emerges from these plants. Our records of Procecidochares atra suggest a similar life cycle, except that adults may overwinter and oviposit on the perenial Solidago as new shoots emerge in the spring. Most temperate species of fruit flies over- winter as diapausing pupae (Bateman 1972). Biological records for some Procecidochares species are questionable because of the need for systematic revision of the genus. Incomplete taxonomy has resulted in species listed by letter designations (Wangberg 1980) or spe- cies listed as near a described taxon (Dodson 1986, 1987). Even with P. atra, which could be considered a well-known species, there is potential for cryptic species. As evidence, Foote et al. (1993) mention that specimens of P. atra from the western part of the range have only one pair of dorsocentral bristles. The more typical pattern is two pairs, with one pair anterior to and the other posterior to the transverse suture. Although two pairs is the more common pattern, we found this character to be extremely variable in our specimens reared from the late spring galls. Almost half of our specimens have three or four setae anterior to the suture (in addition to the posterior pair) and more rarely a single pair anterior and two pairs of setae posterior to the suture. Regardless of the morphological variability in P. atra, it is clear that our May records from Solidago are not for another described species. The only Procecidochares that use Solidago as a host are P. anthracina, P. minuta, and P. polita. Of these, only P. polita (Loew) is an eastern species, and although Vol. 109, No. 3, May & June, 1998 163 about the same size as P. atra, both are relatively easy to differentiate (see Foote et al. 1993). One possibility is that our May records are for a new cryptic species temporally separated from P. atra. Our record on Erigeron may even represent a second new species. Similar to the situation described by Huettel and Bush (1972), the species may have differentiated in their host plant use, but have not diverged morphologically, even though they exist sympatrically. Genetic studies may be useful in determining if our records represent a single bivoltine species with two hosts, two separate species with possibly different hosts, or even three species. ACKNOWLEDGMENTS We foremost thank George Keeney for his collection of the spring galls and his information on the host species and gall location. We also thank John Furlow for his assistance with plant identification and his discussions on Solidago and Andrey Sharkov for the Hymenoptera identi- fication. Our appreciation to two anonymous reviewers whose comments greatly improved the manuscript. LITERATURE CITED Bateman, M. A. 1 972. The ecology of fruit flies. Ann. Rev. of Entomol. 17: 493-518. Benjamin, F. H. 1 934. Descriptions of some native trypetid flies with notes on their habits. U.S. Depart. Agric. Tech. Bull. 401. 95 pp. Bess, H. A. and F. H. Haramoto. 1958. Biological Control of pamakani, Eupatorium adenopho- rum, in Hawaii by a tephritid gall fly, Procecidochares utilis I. The life history of the fly and its effectiveness in the control of the weed. Proc. X Int. Cong. Entom. Montreal 4: 543-548. Christenson L. D. and R. H. Foote. 1960. Biology of fruit flies. Ann. Rev. Entom. 5: 171-192. Dodson, G. 1986. Lek mating system and large male aggressive advantage in a gall-forming tephritid (Diptera: Tephritidae). Ethology 72: 99-108. Dodson, G. 1987. Host plant records and life history notes on New Mexico Tephritidae (Diptera). Proc. Entomol. Soc. Wash. 89: 607-615. Felt, E. P. 1918. Key to American insect galls. N. Y. State Mus. Bull. 200. 310 pp. Foote, R. H., F. L. Blanc, and A. L. Norrbom. 1993. Handbook of the fruit flies (Diptera: Tephritidae) of America north of Mexico. Cornell Univ. Press, Ithaca. 571 pp. Goeden, R.D., D.H. Headrick, and J.A. Teerink. 1994. Life history and descriptions of imma- ture stages of Procecidochares flavipes Aldrich (Diptera: Tephritidae) on Brickellia spp. in southern California. Proc. Entomol. Soc. Wash. 96(2): 288-300. Green, J. F., D. H. Headrick, and R. D. Goeden. 1993. Life history and description of imma- ture stages of Procecidochares stonei Blanc & Foote on Viguiera spp. in southern California (Diptera: Tephritidae). Pan-Pac. Entomol. 69: 18-32. Huettel, M. D. and G. L. Bush. 1972. The genetics of host selection and bearing on sympatric speciation in Procecidochares (Diptera: Tephritidae). Entomol. Exp. Appl. 15:465-480. Novak, J. A., W. B. Stoltzfus, E. J. Allen, and B. A. Foote. 1967. New host records for North American fruit flies. Proc. Entomol. Soc. Wash. 69: 146-148. Phillips, V. T. 1946. The biology and identification of trypetid larvae. Amer. Entomol. Soc. Mem. 12. 161 pp. Silverman, J. and R. D. Goeden. 1980. Life history of a fruit fly, Procecidochares sp., on the ragweed Ambrosia dumosa (Gray) Payne, in southern California (Diptera: Tephritidae). Pan- Pac. Entomol. 56: 283-288. Stebbins, F. A. 1910. Insect galls of Springfield, Massachusetts, and vicinity. Springfield Mus. Nat. Hist. Bull. 2. 138pp. 1 64 ENTOMOLOGICAL NEWS Stegmaier, C. E., Jr. 1968. Erigeron, a host plant genus of tephritids (Diptera). Fla. Entomol. 51:45-50. Tauber, M. J. and C. A. Tauber. 1 968. Biology of the gall-former Procecidochares stonei on a composite. Ann. Entomol. Soc. Amer. 61(2): 553-554. Wangberg, J. K. 1980. Comparative biology of gall-formers in the genus Procecidochares (Diptera: Tephritidae) on rabbitbrush in Idaho. J. Kans. Entomol. Soc. 53(2) 401-420. Wasbauer, M. S. 1972. An annotated host catalog of the fruit flies of America north of Mexico (Diptera: Tephritidae). Occasional Papers No. 19, Bureau of Entomology, Calif. Dept. Agric., Sacramento. 172 pp. BOOK REVIEW WORLD CATALOG OF ODONATA. VOLUME I, ZYGOPTERA; VOLUME II, ANISOPTERA. Henrik Steinmann. 1 997. Walter de Gruyter, Berlin & New York. 1025 plus pp. $870.00 US. These two volumes comprise a straightforward, well-produced, and comprehensive catalog of the worldwide odonate fauna. It attempts to list all known species, arranged according to the taxonomic categories recognized in Davies and Tobin's earlier ( 1 984, 1 985) lists, except that the arrangement of Gomphidae follows Carle ( 1 986). Taxa are listed alphabetically within each higher category (i.e., genera are alphabetical within tribes, etc). Each entry includes a fairly extensive synonomy, although not a complete bibliography of each taxon. This is especially helpful for categories above genus, for which such information often does not come easily to hand. Unfortu- nately, in a number of cases Steinmann cites references in Davies and Tobin as the first entry in a species synonymy rather than citing the actual original description (e.g., - "1985 Gomphurus ozarkensis Westfall, 1975 - loc. cit. Davies & Tobin, ..."). Taxa down to and including tribes are diagnosed briefly, although these descriptions merely repeat those of Davies and Tobin, which sometimes are not, in fact, adequately diagnostic. The type species is listed for each genus as well as the location of the type (if known) and type locality for each species. Species entries also give an indication of the geographic range, although this often is quite general or incomplete. At the end of each volume is a complete index of all names within the corresponding suborder and at the end of Vol. II a selected bibliography totaling about 600 entries (not all synonymic listings appear in the collected bibliography). A certain number of errors have crept in. The type species of Aeshna, e.g., is given as Libellula vulgatissima L. (it is actually L. grandis; L. vulgatissima is later, correctly, cited as the type of Gomphus), and Pseudohagenius is placed as a subgenus of Hagenius rather than of Sieboldius. Warts and all, this work is much as one would expect from a catalog for a medium-sized order of insects and, because it contains substantially more informa- tion than those it is intended to supersede (Davies and Tobin, 1984, 1985;Tsuda, 1 99 1 ), it repre- sents a major improvement on them. Unfortunately, it has appeared about six years too late. I say this because the present work seems to have been done without any reference to (or awareness of?) the excellent catalog of Bridges, first published in 1991, with the third and final edition appearing in 1994, shortly before the author's untimely death. Bridges' work was pri- vately published, but it has been discovered by most serious students of Odonata and has been widely used and cited. It thus seems astonishing that Steinmann's books should take no account of Bridges'. Be that as it may, a comparison is certainly in order, since each work has the same general objectives and each has its strengths and weaknesses. Bridges' catalog organizes and lists family group, genus group, and species group names, each alphabetically within separate sections. A fourth section lists species within genera; genera are again listed alphabetically, but higher classification to the level of subfamily or, when pos- sible, tribe, is indicated in compact form after each genus. Thus Steinmann's work provides a more quickly and easily grasped overview of classification, but the process of finding entries for (continued on page 188) Vol. 109, No. 3, May & June, 1998 165 A SURVEY OF SUMMER TIGER BEETLES ON OHIO RIVER BEACHES IN OHIO AND EASTERN INDIANA1 Gene Kritsky,2 A J. Savage,2 S. Reidel,3 J. Smith2 ABSTRACT: A survey of the riparian tiger beetles of the Ohio River Valley from eastern Ohio to eastern Indiana was conducted during May through August of 1 995 and 1 996. It has provided the first composite analysis of tiger beetle distribution along the river and, combined with the river's recent history, suggested likely causes for declines in riparian tiger beetle populations. The cur- rent distribution of C. repanda and C. cuprascens shows how water management by locks and dams has affected tiger beetle diversity including the extirpation of C. hirticollis from the Ohio River Valley and the reduction of C. marginipennis to one isolated population in eastern Ohio. The survey also found 6 new county records including the easternmost record of C. cuprascens and the first Ohio River record of C. marginipennis. Three species of riparian tiger beetles, Cicindela hirticollis Say, C. cupra- scens LeConte, and C. marginipennis Dejean, are currently listed in the spe- cial interest category by the Ohio Department of Natural Resources Wildlife listings, which means too little is known about their distribution to allow for a true evaluation as to their abundance. These species are sensitive to habitat destruction and in other states have declined or been extirpated (Graves and Brzoska 1991). Previous efforts to map these species' distributions in Ohio (Graves and Brzoska 1991) and in Indiana (Knisley et al 1987) relied prima- rily on museum records and inland collecting efforts. To determine the current status of these riparian species we conducted a beach survey of the Ohio River and its tributaries from eastern Ohio to eastern Indiana. MATERIALS AND METHODS Because the targeted species, C. hirticollis, C. cuprascens, and C. margini- pennis, are summer species, we concentrated our survey during May through early August of 1995 and 1996. The scouting of potential sites was completed by using 1 6 and 1 4 foot speedboats to provide a more accurate coverage of the beaches, bars, and islands along the Ohio River. Once a beach was found, its location was determined using a global positioning system and its tiger beetle fauna was sampled using aerial nets. Voucher specimens were collected and deposited in the Cincinnati Museum of Natural History and Science. 1 Received August 18, 1997. Accepted October 17, 1997. 2 Department of Biology, College of Mount St. Joseph, Cincinnati, OH 45233. 3 Department of Zoology, Miami University, Oxford, OH 45056. ENT. NEWS 109(3) 165-171, May & June, 1998 166 ENTOMOLOGICAL NEWS RESULTS Nearly 300 miles of the Ohio River and its tributaries were surveyed. Tiger beetles were found at 78 sites. The most common species found was C. repanda Dejean (figure 1) which was found throughout the Ohio River Valley. C. cuprascens was less common, occurring in 25 localities (figure 2) primarily in western Ohio and eastern Indiana. C. marginipennis was found in two widely separated locations (figure 3) in Hamilton and Meigs counties in Ohio. C. hirticollis was not found anywhere along the Ohio River. Non-riparian tiger beetles were occasionally collected. C. sexguttata Fabricius was collected on narrow sandy beaches that bordered wooded areas and C. punctulata Oliver was found on beaches that were adjoining farms or other disturbed habitats. Localities and the species collected are provided as an appendix. Indiana West Virginia Figure 1. C. repanda and its Ohio River distribution. Indiana West Virginia Figure 2. C. cuprascens and its Ohio River distribution. Vol. 109, No. 3, May & June, 1998 167 Figure 3. C. marginipennis and its Ohio River distribution. DISCUSSION This two year survey of tiger beetles along the Ohio river marked the first time the Ohio River was so thoroughly surveyed for tiger beetles. C. repanda was collected for the first time in Switzerland and Ohio Counties in Indiana. C. cuprascens was collected for the first time from Switzerland and Ohio Coun- ties in Indiana, Meigs County in Ohio, and from Pleasants County in West Virginia. The West Virginia locality is the farthest east that C. cuprascens has been reported (Acciavatti et al 1992). C. marginipennis was found in Meigs County, Ohio, which was the first time the species has been reported from along the Ohio River. The specific collection information for the new records is presented in bold type in the appendix. C. hirticollis had been collected in Hamilton County, Ohio in 1 9 1 1 (voucher specimen in the Cincinnati Museum of Science and History) but was not found during the two years of our survey. We believe it has been extirpated from southwestern Ohio (Kritsky et al 1996). Graves and Brzoska (1991) warned of declines in populations of C. hirti- collis, C. cuprascens, and C. marginipennis and argued that these species should be better protected. To better understand the specifics of the declines, we stud- ied the history of the Ohio River from eastern Ohio to central Indiana, trying to document changes along the river that would have impacted the abundance of riparian tiger beetles. The shores along the Ohio River have changed a great deal during the past two centuries. The Navigator (Anon 1 8 1 4), a publication of river descriptions created to help boat pilots course the Ohio River, indicated that in the early nineteenth century, the Ohio River ran dry during the summer months. Sandy beaches, sandbars, and willow islands were common along the Ohio River from eastern Ohio through east central Indiana. These locations are ideal 168 ENTOMOLOGICAL NEWS habitats for C. hirticollis, C. cuprascens, and C. repanda. Areas east of Ports- mouth and along what is now Meigs County were apparently more rocky, which is the preferred habitat for C. marginipennis. During the latter part of the Nineteenth Century a series of locks and dams were built on the Ohio River to facilitate river transportation. This construc- tion continued into this century. As new locks were completed, older ones were removed. The most recent locks and dams were completed during the 1990s. The impoundment of water and flood control has had a great effect along the shores of the Ohio. The effect is most severe in eastern Ohio where seven locks and dams are in use. The water impoundment submerged the sandy shores and bars that were present in 1814 and replaced them with high banks. Today, pockets of sandy beaches occur down river of a lock and dam due to the generally lower water levels, and these beaches have dense populations of tiger beetles. The banks five to ten miles up river from a lock and dam are devoid of sandy beaches and thus have no tiger beetles. Figure 4 shows the effects of the locks and dams on tiger beetle distribution. The current locations of locks and dams are indicated by the black lines and the dots show the loca- tions of tiger beetle inhabited beaches. Every lock and dam from eastern Indi- ana to eastern Ohio showed the same effects; a few suitable tiger beetle locali- ties immediately down river from the dam and a large span of up to ten miles up river without suitable tiger beetle habitats. The history of the locks and dams may therefore be the key to understand- ing the recent tiger beetle history in the Ohio River Valley in southwest Ohio. In 1916, there were seven locks and dams in western Ohio and eastern Indiana that have been since removed, and during the time of their operation, western Ohio and Eastern Indiana had higher river levels and fewer beaches for tiger beetles. One victim of this circumstance was C. hirticollis. While the loss of Figure 4. The Ohio River's distribution of the riparian tiger beetles with the locks and dams indicated by black lines. Vol. 109, No. 3, May & June, 1998 169 beaches would have destroyed the tiger beetles' habitat in the area, C. hirticollis was not in a position to recover as were the other species. By comparison, C. repanda is common along the smaller streams and creeks. If its populations along the Ohio River were reduced or obliterated, it could have moved back to the Ohio River from the streams after the removal of the seven locks and dams later in this century. Similarly, C. cuprascens is common on the willow islands found on the Ohio River. While the dams would have caused the submergence of many beaches, the willow islands would have survived and served as refu- gia for C. cuprascens populations to repopulate the reformed beaches after the dams were removed. C. hirticollis was not as fortunate. Unlike C. repanda and C. cuprascens, this species is very sensitive to habitat alterations. In areas along Lake Erie where C. hirticollis is still present, it is only found in areas where human activ- ity is limited. In the late nineteenth century, the streams in southwestern Ohio were heavily polluted, which would have likely destroyed any C. hirticollis populations already present and blocked the dispersal of C. hirticollis upstream as the construction of the many locks and dams in southwestern Ohio pro- ceeded. Therefore, when the Ohio River beaches were lost to high river water, so too was C. hirticollis. Subsequently, when the locks and dams were re- moved, there were no C. hirticollis populations along the streams and creeks that flow into the Ohio to repopulate the beaches of the Ohio River. C. marginipennis presents a completely different scenario. Today, C. marginipennis is found on cobblestone bars and beaches along the Scioto, Great Miami, and Little Miami Rivers. The 1814 Navigator (Anonymous) indicates that it may have had suitable Ohio River habitats east of Portsmouth and in Meigs County. The presence of C. marginipennis in Meigs County may be a remnant of this past habitat. Other cobble beaches along the Ohio River have since been destroyed by the lock and dam systems. CONCLUSIONS The tiger beetle survey of the Ohio River Valley from eastern Ohio to eastern Indiana yielded 6 new county records for three species including the easternmost limit of C. cuprascens and the first Ohio River record of C. marginipennis. The decline in the riparian tiger beetles of the Ohio River is likely linked to the history of the locks and dams used to control flooding and promote river traffic. Suitable tiger beetle habitats are more common immedi- ately down river of locks and dams where river levels are lower. This suggests that river management to protect these sandy beaches would have a positive impact to protect and even promote tiger beetles in the Ohio River Valley. 1 70 ENTOMOLOGICAL NEWS APPENDIX Collection data for the Ohio River riparian tiger beetles. Data is divided by state and presented with county, latitude in decimal degrees, longitude in decimal degrees, species collected, and date of collection. INDIANA: Dearborn, 39.13, 84.8, repanda, 7/2/96; Dearborn, 39.11, 84.83, repanda, 7/2/96; Dearborn, 39. 1 , 84.84, repanda, 7/2/96; Dearborn, 39.07, 84.89, repanda, 7/2/96; Dearborn, 39.34, 84.51, punctulata, 8/5/96; Floyd, 38.28, 85.75, cuprascens, 7/29/95; Jefferson, 38.72, 85.23, repanda, 7/3/96; Jefferson, 38.7, 85.46, repanda, cuprascens, 7/9/96; Jefferson, 38.62, 85.44, repanda, 7/9/96; Ohio, 39.03, 84.88, repanda, 6/27/96; Ohio, 38.99, 84.84, repanda, 6/27/96; Switzerland, 38.87, 84.79, repanda, 6/27/96; Switzerland, 38.78, 85. 0\, repanda, cuprascens, 6/28/96; Switzerland, 38.78, 84.98, cuprascens, 6/28/96; Switzerland, 38.75, 85.04, repanda, 7/3/96. KENTUCKY: Boone, 39.09, 84.66, repanda, cuprascens, 6/9/95; Boone, 39.13, 84.72, repanda, 6/9/95; Boone, 39.13, 84.77, cuprascens, 6/27/95; Boone, 39.14, 84.74, repanda, cuprascens, 6/27/95; Boone, 39.09, 84.66, repanda, punctulata, 7/12/95; Boone, 38.9, 84.86, repanda, 6/27/96; Boone, 38.9, 84.81, repanda, 6/27/96; Boone, 38.96, 84.83, repanda, punctulata, 6/27/96; Boone, 39.1, 84.82, repanda, 7/2/96; Boone, 39.08, 84.85, repanda, cuprascens, 7/2/96; Bracken, 38.8, 84.19, repanda, cuprascens, 6/16/95; Bracken, 38.77, 84.08, sexgutatta, 6/30/95; Bracken, 38.77, 84.02, repanda, 6/30/95; Bracken, 38.77, 84.02, repanda, 6/30/95; Bracken, 38.8, 84.19, cuprascens, 7/25/95; Campbell, 39.05, 84.42, repanda, 6/15/95; Campbell, 39.01, 84.31, repanda, 6/15/95; Campbell, 38.89, 84.24, repanda, 6/16/95; Carroll, 38.7, 85.12, repanda, 7/3/96; Carroll, 38.69, 85.2, repanda, 7/3/96; Lewis, 38.7, 83.55, cuprascens, repanda, 7/13/95; Lewis, 38.65, 83.34, repanda, cuprascens, 7/14/95; Lewis, 38.62, 83.24, repanda, cuprascens, II \ 4/95; Mason, 38.77, 83.9 1 , punctulata, repanda, cuprascens, 7/7/95; Mason, 38.76, 83.87, repanda, 7/7/95; Mason, 38.82, 83.94, repanda, 7/7/95; Timble, 38.6, 85.43, repanda, cuprascens, 7/9/96; Timble, 38.55, 85.41, repanda, 7/9/96; Trimble, 38.73, 85.42, repanda, 7/9/96. OHIO: Adams, 38.69, 83.58, repanda, cuprascens, 7/13/95; Adams, 38.65, 83.64, cuprascens, punctulata, 7/13/95; Adams, 38.63, 83.25, repanda, punctulata, 7/14/95; Adams (Brush Cr. Is.), 38.67, 83.46, repanda, cuprascens, 7/14/95; Brown, 38.73, 83.83, repanda, 7/7/95; Brown, 38.65, 83.72, repanda, cuprascens, punctulata, 7/13/95; Clermont, 39.04, 84.35, repanda, 6/15/95; Clermont, 38.8, 84.2, repanda, cuprascens, 7/25/95; Clermont, 39.1 1, 84.2, repanda, 8/31/96; Hamilton, 39.08, 84.64, repanda, sexgutatta, 6/9/95; Hamilton, 39.08, 84.64, cuprascens, 7/12/95; Hamilton, 39.09, 84.64, repanda, 7/18/95; Hamilton, 39.08, 84.63, repanda, 7/18/95; Hamilton (Great Miami), 39.27, 84.67, repanda, 7/20/95; Hamilton (Great Miami), 39.26, 84.69, repanda, 7/20/95; Hamilton (Great Miami), 39.26, 84.69, repanda, marginipennis, 7/20/95; Hamilton (Great Miami), 39.26, 84.69, repanda, 8/1/95; Hamilton (Whitewater), 39.13, 84.8, repanda, 6/27/95; Hamilton (Whitewater), 39. 1 3, 84.8, repanda, II 1 8/95; Hamilton (Whitewater), 39.13, 84.8, repanda, punctulata, 7/18/95; Hamilton (Whitewater), 39.15, 84.8, repanda, 7/18/95; Hamilton (Whitewater), 39.15, 84.8, repanda, 8/1/95; Meigs, 38.97, 81.92, marginipennis, cuprascens, 7/26/96; Meigs, 38.94, 81 .76, repanda, 7/26/96; Ross, 39.3, 82.94, punctulata, repanda, 7/1 1/96; Scioto, 38.62, 83.22, cuprascens, 7/14/95; Warren (Little Miami), 39.37, 84.15, repanda, 7/27/95. WEST VIRGINIA: Jackson, 38.94, 81.76 cuprascens, 8/1/96; Mason, 38.8, 82.21, repanda, 7/26/96; Pleasants, 39.34, 81.35, cuprascens, 8/1/96; Pleasants, 39.35, 81.32, cuprascens, 8/1/96; Tyler, 39.43, 81.18, repanda, 8/1/96; Wetzel, 39.6, 80.95, repanda, 8/2/96; Wood, 39.27, 81.6, repanda, 8/1/96. Vol. 1 09, No. 3, May & June, 1 998 171 ACKNOWLEDGMENTS We thank Brian Armitage and the Ohio Biological Survey, the Division of Wildlife of the State of Ohio, the College of Mount St. Joseph, and the Indiana Academy of Science for support of this project. This project was also supported with funds donated through the Do Something Wild! state income tax checkoff from the Ohio Division of Wildlife. We also thank Robert Waltz, Indiana Department of Natural Resouces, and two anonymous reviewers for their valuable criti- cism of this paper. LITERATURE CITED Acciavatti, R. E., TJ. Allen, and C. Stuart. 1992. The West Virginia tiger beetles (Coleoptera: Cicindelidae). Cicindela 24 (3-4): 45-78. Anonymous. 1814. The Navigator containing directions for navigating the Monongahela, Allegheny, Ohio and Mississippi Rivers. Pittsburgh; Cramer, Spear, and Eichbaum. Graves, R. C. and D. W. Brzoska. 1991. The tiger beetles of Ohio (Coleoptera: Cicindelidae). Bull. Ohio Biol. Survey (new series) 8(4): vi+42 pp. Knisley, C.B., D. W. Brzoska, and J. R. Shrock. 1987. Distribution, checklist and key to adult tiger beetles (Coleoptera: Cicindelidae) of Indiana. Proc. Indiana Acad. Sci. 97:279-294. Kritsky, G., L. Horner, S. Reidel, and A. J. Savage. 1996. The status of some tiger beetles (Coleoptera: Cicindela spp.) in southern Ohio. Ohio J. Sci. 96:29-30. 1 72 ENTOMOLOGICAL NEWS TICKS OF THE GENUS AMBLYOMMA (ACARI: IXODIDA: IXODIDAE) FROM WHITE-LIPPED PECCARIES, TAYASSU PECARI, IN NORTHEASTERN BOLIVIA, WITH COMMENTS ON HOST SPECIFICITY1 Richard G. Robbins,2 William B. Karesh3 R. Lilian E. Painter^ 5 Susan Rosenberg3 ABSTRACT: Adults of the ixodid ticks Amblyomma cajennense, A. naponense, A. oblongo- guttatum, and A. pecarium are reported from Bolivian populations of the white-lipped peccary, Tayassu pecari. These are the first published records of A. naponense and A. pecarium from Bolivia. Infestations of the three most numerous tick species on T. pecari are shown to be statis- tically independent of host age and sex. It is suggested that in some species of Amblyomma, host specificity may manifest itself chiefly at the preimaginal level, diminishing or disappearing in adults. It has often been noted that ticks of the genus Amblyomma parasitize all classes of terrestrial vertebrates, but that among those specific to mammals, adults are generally more common on large herbivores, while immatures in- fest much smaller mammals (e.g., rodents) or even birds (Hoogstraal 1973, Hoogstraal and Aeschlimann 1982, Hoogstraal 1985). Less often reported are data on the frequency or extent of multi-species assemblages of adult Am- blyomma on large mammal hosts (Fairchild et al. 1966, Matthysse and Colbo 1987, Walker and Olwage 1987). In February of 1996, and again in February of 1997, one of us (WBK) traveled to the Lago Caiman research camp ( 1 3.35S, 60.54W), Noel Kempff Mercado National Park, in the northeastern corner of the Department of Santa Cruz, Bolivia, to assess the health of three herds of 15-40 white-lipped peccaries, Tayassu pecari (Link, 1795), that had been caught as groups in a 0.405 ha capture corral. Tayassu pecari occurs from southern Mexico to northeastern Argentina, but because the large tracts of wilderness on which it depends are rapidly being fragmented, this species has disappeared or become rare in the northern and southern portions of its range and is now listed in appendix 2 (species not necessarily threatened with extinction but that may become so unless trade is subjected to strict regulation) of the Con- vention on International Trade in Endangered Species of Wild Fauna and Flora 1 Received August 7, 1997. Accepted August 30, 1997. 2 Armed Forces Pest Management Board, Walter Reed Army Medical Center, Washington, DC 20307-5001. 3 Field Veterinary Program, Wildlife Conservation Society, 185th Street and Southern Boule- vard, Bronx, NY 10460-1099. 4 Department of Psychology, University of Liverpool, United Kingdom. 5 Proyecto BOLFOR, Santa Cruz, Bolivia. ENT. NEWS 109(3) 172-176, May & June, 1998 Vol. 109, No. 3, May & June, 1998 173 (Mayer and Wetzel 1987, Nowak 1991, Wilson and Reeder 1993). Accord- ingly, we decided to collect all tick specimens found on our Bolivian peccaries in order both to document the diversity of species parasitizing T. pecari at this locale and to determine whether particular host attributes have a bearing on parasitization. Continuing habitat loss will render such analyses impossible within the working lifetime of contemporary investigators. METHODS Over a period of years, one of us (RLEP) developed the Lago Caiman research camp and constructed the peccary capture areas. The lowland forest of Noel Kempff Mercado National Park is broadly classified as subhumid but comprises several forest types; one of these, which includes Lago Caiman, is tall forest with canopy heights of 30-35 m. This part of Bolivia is character- ized by a marked dry season in the austral winter, a mean daily temperature of 25°C, and annual precipitation greater than 1500 mm (Killeen 1996). On 25 February 1996, 13 captured peccaries were randomly targeted for immobilization and tick collection at Lago Caiman. On 17 and 18 February of the following year, an additional 27 peccaries were similarly selected. All pec- caries were sedated using a combination of tiletamine hydrochloride and zolazepam administered by projectile syringe dart. Their pelage was then care- fully searched for ticks, and virtually complete collections were secured from 35 of the 40 animals. Following recovery from anesthesia, all peccaries were released. Ticks were preserved in 70% ethanol and shipped to RGR for identi- fication. By means of contingency tests, it was possible to examine whether tick infestations were dependent on particular attributes of individual peccaries, such as age (adults vs. immatures) and sex. Weight was discounted as a test- able attribute because of its dependence on numerous variables, including sex, health and season. In all cases, the variety of contingency test used was the log likelihood ratio or G-test, with Yates' correction for small sample sizes (Sokal and Rohlf 1 973). Peccaries harboring only preimaginal ticks (larvae, nymphs), which could not be identified to species, were excluded from this analysis. Because all tests were 2x2, computed values of G were compared with a critical value of the chi-square distribution of 3.841 (one degree of freedom) at P = 0.05. RESULTS AND DISCUSSION Adults of four species of Amblyomma -A. cajennense (Fabricius, 1787) (9C?), A. naponense (Packard, 1869) (35Cf , 27 Q ),A. oblongoguttatiim Koch, 1844 (36C\ 409 ). and A. pecarium Dunn, 1933 (359 ) - were found on 31 of 35 parasitized T. pecari at Lago Caiman (four peccaries harbored only Am- blyomma nymphs or larvae). All are relatively common ticks that have previ- 174 ENTOMOLOGICAL NEWS ously been reported from this host (Aragao and Fonseca 1961 , Hoffmann 1962, Fairchild et al. 1966, Jones et al. 1972). Yet, to the best of our knowledge, these are the first published records of A. naponense and A. pecarium from Bolivia. The Field Veterinary Program, Wildlife Conservation Society (formerly New York Zoological Society), has assigned accession numbers WLP 2 through WLP 14 and MED ARKS (Medical Archives) numbers 96-0422 through 96- 0435 to the tick collections made at Lago Caiman in 1996; those made in 1997 have received accession numbers WLP 15 through WLP 40 and MED ARKS numbers 97-0546 through 97-0567. All collections are on long-term loan to RGR. Descriptive statistics for the prevalence and intensity of parasitization by adults of each tick species appear in Table 1 . Typically, ectoparasites are con- tagiously dispersed (overdispersed, clumped) on host populations, a condition in which the zero class is often large (Robbins and Faulkenberry 1982). In the case at hand, of 3 1 sampled peccaries, only 6 were infested by A. cajennense, 1 5 by A. naponense, 23 by A. oblongoguttatum, and 19 by A. pecarium. How- ever, the range of parasitization was broad, as reflected in the disproportion- ately large standard deviations and coefficients of variation of each tick spe- cies. Also, sex ratios (males/females) differed dramatically among the four species of Amblyomma: all male for A. cajennense, 1.29 for A. naponense, 0.90 for A. oblongoguttatum, and all female for A. pecarium, perhaps an indi- cation that these species were sampled at different stages of their life cycles on T. pecari, or that T. pecari is an incidental host for one or more of them. In this regard, it should be noted that while males of A. cajennense and A. pecarium differ markedly in facies, females of the two species are easily confused. There- fore, throughout this study, no female specimen was accepted as A. pecarium unless it met all the differential criteria of Jones et al. (1972): palpal segment II about 2V2 times as long as segment III; festoons ventrally rugose and relatively poorly defined, first 4 on either side of the median festoon each with a well- Table 1 . Descriptive statistics for adults of four species of Amblyomma parasitizing 3 1 individu- als of T. pecari from Lago Caiman, Bolivia, 26 February 1996 and 17-18 February 1997, collec- tor W. B. Karesh. Tick Range Mean with Standard Coefficient of Species (Ticks/Peccary) Standard Error Deviation Variation A. cajennense 0-2 0.3 + 0.1 0.6 200.0 A. naponense 0-34 2.0+ 1.1 6.1 305.0 A. oblongoguttatum 0-13 2.5 + 0.6 3.1 124.0 A. pecarium 0-5 1.1 +0.3 1.4 127.3 Vol. 109, No. 3, May & June, 1998 175 developed tubercle at the posterointernal angle; and internal spur of coxa I broad and blunt. G-test results for the three most numerous tick species on T. pecari appear in Table 2, where the observed statistical independence between tick infesta- tion and host age or sex indicates that factors other than host attributes are responsible for the spectrum of parasitization summarized in Table 1 . Because 53 of the world's approximately 104 Amblyomma species occur in the Neo- tropics, host specificity is almost certainly one such factor. But while adults of A. pecarium seem to be strict parasites of peccaries (Fairchild et al. 1966), adults of A. cajennense, A. naponense and A. oblongoguttatum are known from a variety of large- and medium-sized mammals (Jones et al. 1972). Clearly, our results lend themselves to any number of explanations, yet we suggest that the presence of four species of Amblyomma on but a single species of host may be a sign that host specificity either diminishes or disappears in adults of some amblyommines, manifesting itself instead chiefly at the preimaginal level. However, as has often been stated (Fairchild et al. 1966, Jones et al. 1972, Keirans 1992, Robbins et al. 1997), immature Amblyomma, especially in the Neotropics, remain mostly unidentifiable. Until rearing or molecular genetic studies enable us to associate the immatures found on one set of hosts with the adults found on another, definitive explanations for ostensible instances of host specificity will remain beyond our grasp. Table 2. Tests of association between tick infestation and attributes of Tayassu pecari. Attribute Tick Species Results Age A. naponense Independent; G = 0.370; P » 0.05 A. oblongoguttatum Independent; G = 0.308; P » 0.05 A. pecarium Independent; G = 1 .066; P > 0.05 Sex A. naponense Independent; G = 0.0 1 6; P » 0.05 A. oblongoguttatum Independent; G = 0.086; P » 0.05 A. pecarium Independent; G = 0.348; P » 0.05 ACKNOWLEDGMENTS / We thank the Bolivian National Secretariat for Protected Areas for permission to work in Noel Kempff Mercado National Park, and the National Directorate for the Protection of Biodiversity for help in acquiring the necessary permits. We are extremely grateful to Robert Wallace, Marcela M. Uhart, Nicolas Tagua, Jose Chuvina and Walter "Tirana" Perez, whose dedication and initiative were exemplary throughout this study. Thanks also to Damian Rumiz and the staff of the Bolivian Sustainable Forestry Project (BOLFOR) and to F.A.N. (Fundacion Amigos de la Naturaleza, Santa Cruz, Bolivia) for the logistical support they provided. For their insightful comments on an earlier version of this work, we thank Thomas J. Daniels and Richard 176 ENTOMOLOGICAL NEWS C. Falco of Fordham University's Louis Calder Center in Armonk, New York. And thanks to Lance A. Durden and James E. Keirans, Institute of Arthropodology and Parasitology, Georgia Southern University, Statesboro, for generously providing several important taxonomic refer- ences and for critical assistance in distinguishing A. cajennense from A. pecarium. This study was funded primarily through a grant from the Environmental Program of the Dutch Embassy in Bolivia, by BOLFOR (which is financed by the U.S. Agency for International Development and the Bolivian government), and by the Wildlife Conservation Society. The opinions and asser- tions advanced herein are those of the authors and are not to be construed as official or reflecting the views of the U.S. Departments of the Army or Defense. LITERATURE CITED Aragao, H. de B. and F. O. R. da Fonseca. 1961. Notas de ixodologia. VIII. Lista e chave para os representantes da fauna ixodologica brasileira. Mem. Inst. Oswaldo Cruz 59: 1 15-130. Fairchild, G. B., G. M. Kohls, and V. J. Tipton. 1966. The ticks of Panama (Acarina: Ix- odoidea). pp. 167-219 In: R. L. Wenzel and V. J. Tipton (eds.). Ectoparasites of Panama. Field Mus. Nat. Hist., Chicago. Hoffmann, A. 1962. Monografia de los Ixodoidea de Mexico. I Parte. Rev. Soc. Mex. Hist. Nat. 23: 191-307. Hoogstraal, H. 1973. Acarina (ticks), pp. 89-103 In: A. J. Gibbs (ed.). Viruses and invertebrates. North Holland Publ. Co., Amsterdam. Hoogstraal, H. 1985. Ticks, pp. 347-370 In: S. M. Gaafar, W. E. Howard, and R. E. Marsh (eds.). Parasites, pests and predators. Elsevier Sci. Publ. Co., Amsterdam. Hoogstraal, H. and A. Aeschlimann. 1 982. Tick-host specificity. Mitt. Schweiz. Entomol. Ges./ Bull. Soc. Entomol. Suisse 55: 5-32. Jones, E. K., C. M. Clifford, J. E. Keirans, and G. M. Kohls. 1972. The ticks of Venezuela (Acarina: Ixodoidea) with a key to the species of Amblyomma in the Western Hemisphere. Brigham Young Univ. Sci. Bull. (Biol. Ser.) 17: 1-40. Keirans, J. E. 1992. Systematics of the Ixodida (Argasidae, Ixodidae, Nuttalliellidae): an over- view and some problems, pp. 1-21 In: B. Fivaz, T. Petney, and I. Horak (eds.). Tick vector biology. Medical and veterinary aspects. Springer- Verlag, Berlin. Killeen, T. J. 1 996. Historia natural y biodi versidad de Parque Nacional "Noel Kempff Mercado," Santa Cruz, Bolivia. Plan de manejo - components cientifico. Museo de Historia Natural Noel Kempff Mercado. Matthysse, J. G. and M. H. Colbo. 1987. The ixodid ticks of Uganda. Entomol. Soc. Am., College Park, MD. Mayer, J. J. and R. M. Wetzel. 1987. Tayassu pecari. Mammalian Species (293): 1-7. Nowak, R. M. 1991. Walker's mammals of the world. 5th ed. Johns Hopkins Univ. Press, Balti- more. Robbins, R. G. and G. D. Faulkenberry. 1982. A population model for fleas of the gray-tailed vole, Microtus canicaudus Miller. Entomol. News 93: 70-74. Robbins, R. G., W. B. Karesh, S. Rosenberg, N. Schonwalter, and C. Inthavong. 1997. Two noteworthy collections of ticks (Acari: Ixodida: Ixodidae) from endangered carnivores in the Lao People's Democratic Republic. Entomol. News 108: 60-62. Sokal, R. R. and F. J. Rohlf. 1973. Introduction to biostatistics. W. H. Freeman and Co., San Francisco. Walker, J. B. and A. Olwage. 1987. The tick vectors ofCowdria ruminantium (Ixodoidea, Ixo- didae, genus Amblyomma) and their distribution. Onderstepoort J. Vet. Res. 54: 353-379. Wilson, D. E. and D. M. Reeder. 1993. Mammal species of the world. A taxonomic and geo- graphic reference. 2nd ed. Smithson. Inst. Press, Washington, DC. Vol. 1 09, No. 3, May & June, 1 998 1 77 NEW RECORDS OF JAPYGOIDEA (HEXAPODA: DIPLURA) FROM LOUISIANA, WITH NOTES ON BEHAVIOR1 Mark A. Muegge? Christopher E. Carlton^ ABSTRACT: Previous published records of the hexapod order Diplura from Louisiana have documented one species. We provide collection data for seven species of Japygoidea: five within Japygidae and two within Parajapygidae. Two genera and four species of Louisiana Japygidae are undescribed. Members of Japygidae are saprophagous and predatory in feeding behavior. Cerci were not used during any observations of prey capture, but were employed defensively as pincers. Japygoids are a primitive group of blind, flightless hexapods belonging to the class Diplura. The bionomics of this obscure group of hexapods remain poorly known. Japygoids have been recorded primarily from mesic habitats beneath rocks, rotting logs, leaf litter, humus, and soil. Some species occur in xeric habitats and three species are obligate cavernicoles (Muegge, 1992; Pages, 1972, 1977). Japygids are prone to extreme endemism (Allen, 1988; Muegge, 1992; Muegge and Bernard, 1989; Smith, 1960). Their relatively small size, wing- less and eyeless anatomy, and the occurrence of most species in edaphic habi- tats contribute to this tendency toward endemism. Thus, while Diplura and similar cryptic organisms could be useful in providing insight into biogeo- graphic patterns, particularly in identifying areas of endemism, an almost total lack of information about their distributions and regional diversity is a barrier to accomplishing this goal. The information presented here is part of a con- tinuing effort to document the distributional patterns and systematic status of Japygoidea in North America and provide information about their biology. Voucher specimens of taxa reported here are deposited in the Louisiana State Arthropod Museum and the first author's collection. BEHAVIORAL NOTES Observations of behavior and examination of gut contents by one of us (MAM) indicate that Japygoids are generally saprophagous and predatory, pursuing and consuming live prey opportunistically. Examinations of the gut 1 Received August 18, 1997. Accepted September 22, 1997. 2 Texas A & M University, District 6 Extension Center, P.O. Box 1 298, Ft. Stockton, TX 79735 USA. 3 Christopher E. Carlton, Louisiana State University Agricultural Center, Department of Ento- mology, Baton Rouge, LA 70803 USA. ENT. NEWS 109(3) 177-182, May & June, 1998 1 78 ENTOMOLOGICAL NEWS contents of numerous specimens of several species revealed an abundance of arthropod body parts, primarily from springtails and mites, and a significant amount of undigested, unrecognizable organic matter. The japygid Metajapyx remingtoni Smith and Bolton was observed during prey stalking and capture. The long musculated antennae were used to search for prey. Once a potential prey item was detected, the animal slowly crept to within striking distance, then lunged forward to capture, subdue, and consume the prey. Individuals of M. remingtoni were observed capturing and consum- ing entomobryid springtails. The feeding habits of Japyx sp. were studied by Pages ( 1 95 1 ), who reported predation on an isopod, Platyarthrus hoffmanseggti Brandt and the following gut contents: isopods, mites (mainly oribatids and gamasids), Symphyla, japygids, Diptera larvae, adult beetles, vegetable de- bris, and mycelia. Schaller (1968) described the use of forceps during prey capture and depicted it in a pair of drawings, but provided no observational data or references to support the description. Kuhnelt ( 1 976) published a simi- lar drawing depicting the capture of a campodid dipluran using the forceps. Finally, Conde and Pages (1991) reported that individuals of Heterojapyx sp. were observed buried in soil with cerci exposed, waiting to capture small arthropods that came within reach using the cerci. However, they did not state whether they observed the cerci being used in this manner. Thus, there are numerous reports in the literature suggesting that japygoids use their cereal forceps during prey capture, but these reports are not sup- ported by detailed observational data. We have observed forceps being used in defensive behavior on several occasions, but never during prey capture. The tenth abdominal segment and forceps are heavily sclerotized, musculated, and quite powerful considering the size of the animal. Japygids under duress were observed grasping and completely severing the bodies of other similar sized arthropods using the forceps. These animals were never consumed during our observations. NEW RECORDS FROM LOUISIANA (Fig. 1) Family JAPYGIDAE Mixojapyx tridenticulatUS (Fox). Specimens examined, 21. Avoyelles Parish, near Hamburg, 2 July 1979, H. Lambert, habitat: in soil, 1 female. East Feliciana Parish, Idlewild Research Station, 15 January 1989, M. A. Muegge, habitat: in moist soil at base of Quercus sp., mixed oak/pine forest, 1 male, 1 female. Same data, 3 June 1989, base of Pinus sp. 1 male. Same data, 23 January 1990, 1 male. Same data, 1 March 1990, 1 female. Same data, 13 February 1 99 1 , 2 males, 4 females. Grant Parish, Kisatchie National Forest, 29 March 1 992, M. A. Muegge, habitat: moist soil, mixed beech/magnolia forest, 1 male, 3 females. Natchitoches Parish, Kisatchie National Forest, Red Dirt National Wildlife Management Area, 1 April 1989, M. A. Muegge, habitat: in soil beneath rock, primarily long-leaf pine forest. Same data, 10 June 1991, M. A. Muegge, 1 female. St. Landry Parish, Thistlewaite Wildlife Management Area, 14 January 1989, M. A. Muegge, habitat: in moist sandy soil, pine/oak forest, 1 male, 1 female. Same data, 27 Vol. 109, No. 3, May & June, 1998 179 January 1990, 1 female. Washington Parish, near Southeast Research Station, 25 January 1990, M. A. Muegge, habitat: about 25 cm deep in moist soil near Pinus sp. along roadside, 1 female. Range. Gulf Coast States. Comments. Mixojapyx tridenticulatus is the only species of this primarily Mexican genus found in Louisiana. It has the widest distribution of any Mixojapyx species north of Mexico, occurring in the gulf coastal region from east Texas to Florida. The specimens examined from east of the Mississippi River display slight, but consistent chaetotaxic differences from those west of the Mississippi River. Upon further investigation, these two populations may be found to represent distinct species. Metajapyx undescribed species 1. Specimens examined. 26. East Feliciana Parish, Idlewild Research Station, 1 March 1990, M. A. Muegge, habitat: moist soil, mixed oak/pine forest, 2 males, 5 females. Same data, 27 February 1990, 3 males, 3 females. Same data, 1-3 March 1991, 4 males, 7 females. Same data, 13 February 1991, except habitat, under rotting log near stream bank, 1 male, 1 female. Range. Known only from the above locality. Comments. Metajapyx is a widespread genus reported almost exclusively from locations east of the Mississippi River (Reddell, 1983). Rathman et al. (1988) reported an undescribed Metajapyx species from eastern Washington State, but some characters needed to confirm the identification were not described. Thus, further study will be required to determine its correct generic placement. Fox (1941) and Smith (1960) reported M. subterraneus from Oregon and Stoddard counties, Missouri, respectively, and these are the only reliable records of the genus west of the Mississippi River. In Louisiana, speci- mens of Metajapyx have only been found east of the Mississippi River. Metajapyx undescribed species 2. Specimens examined, 17. East Feliciana Parish, Idlewild Research Station, 3 June 1989, M. A. Muegge, habitat: in moist soil and litter at base of Pinus sp, mixed oak/pine forest, 1 male, 16 females. Range. Known only from the above locality. Comments. This and the preceding species are being described in a revi- sion of Metajapyx currently underway. Undescribed genus and species 1. Specimens examined, 1 . Caddo Parish. Shreve- port, 4634 Dixie Blvd., 12 December 1993, J. T. McBride and V. L. Moseley, habitat: under rotting wood in urban backyard, 1 male. Range. Known only from the above locality. Comments. The discovery of this undescribed genus in an urban habitat was unexpected. Chaetotaxy, structure of the subcoxal organs, and structure of the cerci suggest that this individual represents a distinct and undescribed genus. Undescribed genus and species 2. Specimens examined, 1 . Webster Parish, Kisatchie National Forest, 22 March 1990, M. A. Muegge, habitat: soil at base of Pinus sp., moist mixed oak/pine forest, 1 male. 1 80 ENTOMOLOGICAL NEWS Range. Ouachita Highlands of eastern Oklahoma and Arkansas, south to northwestern Louisiana. Comments. Many specimens representing several species in this uncle- scribed genus have been collected by the authors and others from Arkansas and Oklahoma. The individual collected in northern Louisiana may represent the southernmost limit of the genus' range. Further study is underway to deter- mine the number of species in the genus. Family PARAJAPYGIDAE Parajapyx (Parajapyx) isabellae (Grassi). Specimens examined, 62, not sorted by sex. Acadia Parish, 15 October 1964, W. Sonnier, habitat: soil from sweet potato field. Acadia Parish, LSU Rice Research Station near Crowley, 12 June 1994, M. A. Muegge, habitat: soil sample from rice field. Avoyelles Parish, near Hamburg, 2 July 1979, H. Lambert, habitat: soil, 1 specimen. Bienville Parish, near Lake Bistineau, 1 August 1990, M. A. Muegge, habitat: soil, mixed oak/pine forest, 2 specimens. East Baton Rouge Parish, 11 March 1982, P. J. Barbour, habitat: soil/leaf litter berlesate. East Feliciana Parish, Idlewild Research Station near Clinton, 28 May 1989, M. A. Muegge, habitat: sandy soil near stream, mixed beech/magnolia forest, 6 specimens. Same data, 30 July 1989, 1 1 specimens. Same data, 9 February 1991, 12 specimens from sandy soil near stream, 9 specimens from soil near decaying log. Same data, 30 June 1 991 , soil near Pinus sp. St. Landry Parish, 15 July 1979, C. E. Eastman, habitat: soil. Same, except near Port Barre, 22 June 1 982, 2 specimens. Webster Parish, Kisatchie National Forest, 22 March 1990, M. A. Muegge, habitat: in soil near Pinus sp., mixed forest, 2 specimens. Range. Cosmopolitan. Comments. The only previously published references to Japygoids from Louisiana were made by Ingram (1931), Ingram etal. (1950), and Fox (1957). These specimens were reported only as Japyx sp. Ingram ( 1 93 1 ) and Ingram et al. (1950) reported that specimens of Japyx sp. were commonly found in sug- arcane fields, and suggested that they could be potential pests, causing dam- age by feeding on the root systems of the plant. Although we have not exam- ined any specimens collected by Ingram, we agree with Reddell (1983) that these records represent P. isabellae. This species is widespread and common, and is the only japygoid that is commonly collected in agricultural monocul- tures. Parajapyx (Grassjapyx) grassianus "maiusculella" Silvestri. Specimens ex- amined, 12. East Feliciana Parish, Idlewild Research Station near Clinton, 9 February 1989, M. A. Muegge, habitat: sandy soil near stream, mixed beech/magnolia forest, 2 specimens. Same data, 30 July 1989, 1 specimen. Same data, 30 July 1991, soil at base of Pinus sp., 2 specimens. Same data 9 February 1991, 7 specimens. Range. Coastal Louisiana to Florida. Comments. The taxonomic status of the P. grassianus is uncertain. The type locality for P. grassianus is Cordoba, Veracruz, Mexico (Silvestri, 1911) and it has been recorded only from locations in Mexico. Silvestri (1948) sub- sequently described one form, "forma vel mutans", and two variations of that form, "maiusculella" and "robustior", from Florida These descriptions were based primarily on cereal dentation. Based on cereal dentation, the specimens Vol. 109, No. 3, May & June, 1998 181 O Mixojapyx tridenticulatus . Metajapyx undescribed sp 1 Metajapyx undescribed sp 2 Undescribed genus & sp 1 Undescribed genus & sp 2 Parajapyx isabellae Parajapyx grassianus Figure 1. Collecting localities of Japygoidea recorded from Louisiana. 1 82 ENTOMOLOGICAL NEWS from Louisiana represent the variant "maiusculella". Further study is neces- sary to determine if the Florida and Louisiana specimens represent undescribed species, or geographical variants of P. grassianus. ACKNOWLEDGMENTS Publication of this manuscript was supported in part by a grant to the second author from the Louisiana Education Quality Support Fund. We thank an anonymous reviewer for calling our attention to several pertinent references. LITERATURE CITED Allen, R. T. 1988. A new species of Occasjapyx from the Interior Highlands (Insecta: Diplura: Japygidae). Proc. Ark. Acad. Sci. 42: 22-23. Conde, B., and J. Pages. 1991. Diplura. pp. 269-271. In. CSIRO (ed.), The Insects of Australia: A Textbook for Students and Research Workers (2nd ed.). Vol. 1. Cornell Univ. Press, Ithaca, NY. Fox, I. 1941. New or little known North American Japygidae. Can. Entomol. 73: 28-31. Fox, I. 1957. The insect family Japygidae (order Thysanura) in Puerto Rico. J. Agr. Univ. Puerto Rico 41: 35-37. Ingrain, J. W. 1931. Soil animals attacking sugarcane. J. Econ. Entomol. 24: 866-869. Ingram, J. W., E. K. Bynum, L. J. Charpentier, and W. E. Haley. 1950. Chemical control of soil insects and organisms attacking sugarcane. Sugar Journal 12: 13-14, 20. Kuhnelt, W. 1976. Soil Biology. Faber and Faber, London. 483 pp. Muegge, M. A. 1992. New species of cavernicolous japygid (Japygidae: Diplura) from Texas. Ann. Entomol. Soc. Amer. 85: 40-412. Muegge, M. A., and E. C. Bernard. 1990. Two new species of Me tajapyx (Diplura: Japygidae) from Tennessee. Proc. Entomol. Soc. Wash. 92: 793-801. Pages, J. 195 1 . Contribution a la connaissance des Diploures. Bull. Scientifique de Bourgogne 13, Supplement 9: 1-97, 12 pis. Pages, J. 1972. Les Japygidaes cavernicoles de la francaise. Int. J. Speleol. 4: 61-66. Pages, J. 1977. Dicellurata genavensia III. Japygides du Sud-Est. asiatique n° 1. Rev. Suisse Zool. 84: 687-698. Rath man, R. J., R. D. Akre, and J. F. Brunner. 1988. External morphology of a species of Metajapyx (Diplura: Japygidae) from Washington. Pan-Pacific Entomol. 64: 185-192. Reddell, J. R. 1 983. A checklist and bibliography of the Japygoidea (Insecta: Diplura) of North America, Central America, and the West Indies. Texas Mem. Mus., Pearce-Sellards Ser., no. 37. 41 pp. Schaller, F. 1968. Soil Animals. The University of Michican Press, Ann Arbor. 144 pp. Silvestri, F. 1911. Materiali per lo sstudiodeiTisanuri. XII. Un novo genere e undici specie nove di Japygidae dell 'America settentrionale. Boll. Lob. Zool. Gen. Agr. Protici 5: 72-87. Silvestri, F. 1948. Intorna ad alcune anomalie di Japygidae (Insecta, Diplura). Boll. Lab. Ento. Agr. Portici 8: 209-213. Smith. L. M. 1960. Japygidae of North America 7. A new genus in the Provalljapyginae from Missouri. Proc. Biol. Soc. Wash. 73: 261-266. Vol. 109, No. 3, May & June, 1998 183 NEW RECORDS OF ALLOCAPNIA (PLECOPTERA: CAPNIIDAE) FROM MISSISSIPPI AND LOUISIANA, WITH ACCOMPANYING SCANNING ELECTRON MICROGRAPHS1 Mac H. Alford2 ABSTRACT: Seventy-one sites were surveyed for Alloc apnia (Plecoptera: Capniidae) in south- western Mississippi and three of the Florida parishes of Louisiana. These surveys extended over six major river drainages. New records of A. virginiana, A. recta, and A. aurora are reported, and scanning electron micrographs of the male genitalia are provided for each species. Small winter stoneflies (Capniidae) constitute the largest family of Plecop- tera in North America (Borror, Triplehorn, and Johnson 1 989). They have cap- tivated curious minds at least since the mid-nineteenth century (Prison 1929) because they are generally less than 10 mm in length and, unlike most other insects, emerge as adults during the winter or early spring (Ross and Ricker 1 97 1 ). Most capniids in eastern North America belong to the genus Allocapnia Claassen (Borror, Triplehorn, and Johnson 1989). Allocapnia is typical of the Capniidae and is distinguished from the other genera of the family by the pres- ence of a straight RI of the fore wing just beyond the origin of Rs (Stewart and Harper 1996) and by the presence of a dorsal process on the eighth tergite and a double epiproct in males (Ross and Ricker 1971). Differentiation of species is chiefly based on characteristics of the male genitalia. METHODS Adult Allocapnia were collected from December 1996 to February 1997 by hand from bridges and by rod and beating sheet from streamside plants. Nymphs were collected by browsing through leaf litter in streams. Specimens were killed and stored in 80% ethanol and were examined and identified using a dissecting microscope. The genitalia of representative male samples were then viewed using an AMRAY 1810D scanning electron microscope and pho- tographed. Specimens were deposited in the entomological collection of B. P. Stark at Mississippi College. Forty-eight sites were surveyed in the southwestern Mississippi counties of Amite, Franklin, Lincoln, Pike, Walthall, and Wilkinson. Twenty-three sites were surveyed in three of the Florida parishes of Louisiana, namely, East Feliciana, St. Helena, and West Feliciana. The Florida parishes of Louisiana 1 Received July 18, 1997. Accepted September 8, 1997. 2 Department of Biological Sciences, Mississippi College, Clinton, MS 39058-4045. Current address: Department of Botany, Duke University, Durham, NC 27708-0338. ENT. NEWS 109(3) 183-188, May & June, 1998 184 ENTOMOLOGICAL NEWS are those that occur in the southeastern part of the state east of the Mississippi River. The surveys included collection sites from the Amite River, Bogue Chitto River, Buffalo River, and Homochitto River drainages in Mississippi and the Bayou Sara and Thompson Creek drainages in Louisiana. Also, one small stream in Mississippi and two small streams in Louisiana which flow directly into the Mississippi River were surveyed (Fig. 1). Fig. 1 . Map of the study area: southwest Mississippi and three of the Florida parishes of Louisi- ana. Sites with Allocapnia records in the winter of 1996-1997 are indicated by a H and negative records are indicated by a A. Numbered collection sites are further described in the text. RESULTS AND DISCUSSION Fig. 1 is a summary map of the sample localities. Sites with Allocapnia are numbered as follows: 1 . Wilkinson Co., MS, Clark Cr., Clark Cr. Natural Area, A. recta. 2. West Feliciana P., LA, Kimball Cr. at Pinckneyville Rd., unresolved 9 (likely A. recta). 3. West Feliciana P., LA, Dry Cr. at LA 421, A. recta. 4. West Feliciana P., LA, Mill Cr. at LA 421, A. recta. 5. West Feliciana P., LA, Thorn Cr. at LA 421, A. recta. 6. West Feliciana P., LA, un-named tributary to Middle Fork Thompson Cr. at LA 421, unre- solved 9 (likely A. recta). Vol. 109, No. 3, May & June, 1998 185 7. East Feliciana P., LA, Hurricane Cr. at Thompson Cr. Rd., A. recta, A. virginiana. 8. East Feliciana P., LA, Shady Grove Branch at Thompson Cr. Rd., A. recta, A. virginiana. 9. Wilkinson Co., MS, Buffalo R. at Hiram McGraw Rd., A. recta, A. virginiana. 10. Amite and Wilkinson Co., MS, Foster Cr. at MS 33, nymph. 1 1 . Amite Co., MS, Foster Cr. at MS 33, A. aurora, A. virginiana. 12. Amite Co., MS, Brushy Cr. at Cobb Rd., A. virginiana. 13. Amite Co., MS, Brushy Cr. at New Hope Rd., A. virginiana. 14. Amite Co., MS, un-named tributary to Birdman Branch at Fox Rd., A. recta. 15. Amite Co., MS, Caston Cr. at Oxford-Meadville Rd., A. virginiana. 16. Franklin Co., MS, Middleton Cr. at USFS 100, A. virginiana. 17. Franklin Co., MS, Porter Cr. at USFS 108, A. recta, A. virginiana. 18. Franklin Co., MS, Dry Cr. at USFS 145, A. aurora, A. recta, A. virginiana. 19. Franklin Co., MS, McGehee Cr. at low-water bridge, A. virginiana. 20. Franklin Co., MS, Cane Mill Branch near Little Springs, A. aurora, A. virginiana. 21. Franklin Co., MS, Goober Cr. at Little Springs, A. virginiana. 22. Amite Co., MS, un-named tributary to West Fork Amite River, A. recta. Of the seventy-one surveyed sites, twenty-two (31%) yielded at least one specimen of Allocapnia. The majority of these sites (82%) occur in the Homochitto River drainage of Mississippi and the Thompson Creek drainage of Louisiana. No specimens of Allocapnia were collected from the Bogue Chitto River drainage. Specimens were collected from one site in the Amite River drainage, one site in the Buffalo River drainage, one site in the Bayou Sara drainage, and one site from a stream which flows directly into the Mississippi River. Overall, 188 adults (151 Cf , 37 Q ) and 14 nymphs were collected. Of the identifiable specimens (some dehydrated individuals were collected from spi- der webs and some females could not be resolved), 84 (56%) belonged to Allocapnia virginiana Prison, 62 (41%) to A. recta (Claassen) Prison, and 4 (3%) to A. aurora Ricker. Scanning electron micrographs are provided in Figs. 2-7. A. virginiana (Figs. 2-3) is characterized by a large apical segment of the upper limb of the epiproct and by a wide but short dorsal process with a small anterior process. A. recta (Figs. 4-5) is characterized by a long, thin apical segment and a dorsal process with a semicircular apical ridge. A. aurora (Figs. 6-7) is characterized by a slender apical segment and a dorsal process with widely separated lobes. Although the data imply a paucity of Allocapnia outside of the Homochitto River and Thompson Creek drainages, this is somewhat misleading. Two of the three streams which flow directly into the Mississippi River probably did not produce Allocapnia individuals because occasional inundation from the Mississippi River hinders nymphal establishment. The Bayou Sara drainage experiences similar effects from the Mississippi River. Clark Creek, on the other hand, has several high waterfalls in the loess bluffs region and is pro- tected from periodic flooding and backwater. The Buffalo River probably has more Allocapnia than reported. Of the four sites investigated, three fall within company timberland, where the streams have litter composed primarily of pine straw. The Amite River and Bogue Chitto 186 ENTOMOLOGICAL NEWS Fig. 2. Allocapnia virginiana epiproct, dorsal aspect. Fig. 3. Allocapnia virginiana epiproct, lateral aspect. Fig. 4. Allocapnia recta epiproct, dorsal aspect. Fig. 5. Allocapnia recta epiproct, lateral aspect. Fig. 6. Allocapnia aurora epiproct, dorsal aspect. Fig. 7. Allocapnia aurora epiproct, lateral aspect. Vol. 109, No. 3, May & June, 1998 187 River, however, are somewhat different. Whereas the previous drainages con- sist primarily of fast-flowing, shallow, sandy streams, the Amite River and Bogue Chitto River drainages consist of deep, slow-flowing, mixed gravel-silt streams. The single record from the Amite River drainage indicates that Allo- capnia are present and suggests that they are piobably limited to localized populations where stream habitat is suitable. Further searches in the Bogue Chitto River drainage are necessary to determine the presence or absence of Allocapnia there. These data indicate a wide distribution of both A. recta and A. virginiana. A. recta appears to predominate in Louisiana while A. virginiana appears to predominate in the hilly Homochitto drainage of Mississippi. This apparent discrepancy, however, may be due to collection and emergence timing. B. P. Stark (pers. comm.) has previously collected both species in central Missis- sippi and A. virginiana in the Homochitto drainage. Ross and Ricker (1971) report neither species in Louisiana or southwest Mississippi, but this may be due to little or no sampling in the area. They do, however, report collections of A. virginiana from eastern Mississippi and note the abundance of A. recta in the Coastal Plain (Ross and Ricker 1971). Allocapnia aurora was only found in the Homochitto drainage in a few disjunct locations. Stark (pers. comm.) has not collected A. aurora southwest of Tishomingo Co., MS, despite intensive collecting in central Mississippi, and Ross and Ricker (1971) report it no farther west than southwestern Ala- bama. This interesting anomaly of distribution could be the result of dispersal via the Pliocene Tennessee River entering the retreating Mississippi Embayment near southwest Mississippi during a glacial advance. Stern (1976) provides evidence for such an event based on unionid mussel distributions and sums up the geologic evidence. Why no A. aurora occur between southwest Missis- sippi and the southern Appalachians or southwestern Alabama, nevertheless, remains unclear. Regardless, because distributional information is often inte- grated with phylogeny to construct hypothetical dispersal paths and to specu- late about selection pressures, these new records are valuable to an accurate evolutionary understanding of Allocapnia. ACKNOWLEDGMENTS Mississippi College is thanked for use of the scanning electron microscope facilities, and Dr. Bill Stark at Mississippi College is thanked for verifying species identifications and for help- ful comments. Earl Alford of the Mississippi Forestry Commission is thanked for helping to select appropriate collection sites. LITERATURE CITED Horror, D. J., C. A. Triplehorn, and N. F. Johnson. 1989. An Introduction to the Study of Insects, 6th ed. Harcourt Brace Jovanovich, Fort Worth. 1 88 ENTOMOLOGICAL NEWS Prison, T. 1929. Fall and Winter Stoneflies, or Plecoptera, of Illinois. Bull. 111. Nat. Hist. Surv., 18:345-361,399-403. Ross, H. H. and W. E. Ricker. 1 97 1 . The Classification, Evolution, and Dispersal of the Winter Stonefly Genus Allocapnia. 111. Biol. Monog., 45: 1-166. Stern, E. M. 1976. The Freshwater Mussels (Unionidae) of the Lake Maurepas-Pontchartrain- Borgne Drainage System, Louisiana and Mississippi. Unpubl. Ph.D. Thesis, LA State Univ. Stewart, K W. and P. P. Harper. 1996. Plecoptera. pp. 217-266 In: Merritt, R. W. and K. W. Cummins, eds. An Introduction to the Aquatic Insects of North America, 3rd ed. Kendall/ Hunt, Dubuque, IA. BOOK REVIEW (continued from page 1 64) given genera and species is not so transparent (the indices ameliorate the problem to a consider- able extent, however). It should be noted that the classification of Odonata at the level of sub- families and tribes is currently not very well-founded and is in a state of flux, although this is certainly no fault of either author. In general, the Steinmann volumes use a much more traditional format, so entomologists accustomed to using catalogs for other groups may feel more at home with them, but I have found that, with a little initial effort, Bridges' catalog is extremely easy to use and provides a number of advantages over the common approach. On the other hand, Steinmann certainly is physically easier to use, as it is printed as two compact volumes, while Bridges is a rather un- wieldy tome. Steinmann also provides a slightly more extensive synonymy, and the full refer- ences appear with the species to which they pertain, whereas Bridges' citations are numerically coded and the full references appear only in the bibliography. The latter is by far the more exten- sive and up-to-date, however, with over 6500 entries, and it is accompanied by indices that allow cross-referencing by author and journal. This in itself is an outstandingly useful reference. Bridges provides no indication of range nor does he include diagnoses of any taxa, but the last edition does contain an appendix with figures of wings of most genera. The major failing of Steinmann 's World Catalog, especially compared to Bridges', how- ever, is that it simply is not current. It has used Davies and Tobin (1984, 1985) as its starting point, but, although it has added substantially to their work, it has not moved beyond that as it could and should have. Despite considerable, and accelerating, work on the taxonomy of the Odonata over the last decade, almost no species described after 1990 are included here. Also missed were, e.g., Lohmann's (1992) revision of the Cordulegastridae, and the major reanalysis by Carle and Louton (1994) separating a new family, the Austropetaliidae, from the now mono- typic Neopetaliidae, with a radically new understanding of the position of the latter. Thus, de- spite the 1997 publication date, this catalog lags considerably behind Bridges' 1994 edition. Coupled with its astronomical price (over $850 at current exchange rates), this makes it hard to recommend as a practical tool for the study of Odonata, despite its several useful features. LITERATURE CITED Bridges, C. A. 1 994. Catalogue of the Family-group, Genus-group and Species-group Names of the Odonata of the World (Third Edition). Urbana, IL, privately published. Carle, F. L. 1986. The classification, phylogeny and biogeography of the Gomphidae (Anisop- tera). I. classification. Odonatologica 15: 275-326. Carle, F. L., & J. A. Louton. 1994. The larva of Neopetalia punctata and establishment of Austropetaliidae fam. nov. (Odonata). Proc. Ent. Soc. Wash. 96: 147-155. (concluded on page 194) Vol. 109, No. 3, May & June, 1998 189 FIRST UNITED STATES RECORD OF DYSCHIRIUS SEXTONI (COLEOPTERA: CARABIDAE)1 Foster Forbes Purrington? Judith A. Maxwell^ ABSTRACT: We report the first United States capture of the ground beetle, Dyschirius sextoni, at Fort McCoy Military Reservation, Monroe County, Wisconsin (16 July 1996). In addition we newly record from Wisconsin the ground beetles, Harpalus indianus, H. indigens, Selenophorus planipennis, Cymindis ptanipennis and Helluomorphoides praeustus bicolor. Fort McCoy Military Reservation in northern Monroe County, Wisconsin is characterized by glacial outwash plains, with extensive unconsolidated strati- fied surface gravel and sand. Annual total mean precipitation (1937 to 1959) in Monroe County is 71 cm (Barndt and Langton 1984). This area is located within a circumscribed upper Midwestern drift-less region that remained ice- free during the Wisconsinan glaciation (Curtis 1959). Vegetational development at Fort McCoy is dominated by scrubby oak barrens, a Quercus velutina Lam. and Q. ellipsoidalis E.J. Hill co-climax, with associated forbs and grasses such as Lupinus perennis L., Andropogon gerardi Vitman, Rudbeckia hirta L. and Baptisia leucophaea Nutt. Scars from military tank traffic persist in many sand and gravel areas, and sand borrow pits present colonization opportunities for several open ground specialist carabid species like tiger beetles. The carabid fauna of tallgrass prairie in the physiographically anomalous drift-less zone was studied by Purrington and Larsen (1996) in northeastern Iowa. MATERIALS AND METHODS Water-filled yellow pan traps and automatic blacklight traps (BioQuip, Inc.) were used from May through August in 1995 and 1996 to assay the insect fauna of the unglaciated oak barrens habitat of Fort McCoy. Ground beetles were taken as a consequence of this broad-based synoptic survey. Voucher specimens of all ground beetle species obtained are held in the University of Wisconsin Department of Entomology Research Collection in Madison. RESULTS Sixty-seven species of ground beetles in 15 tribes were obtained in Mon- 1 Received 9 September, 1997. Accepted 29 October, 1997. 2 Department of Entomology, The Ohio State University, 1 735 Neil Avenue, Columbus OH 432 1 0. 3 Department of Entomology, University of Wisconsin, 237 Russell Laboratories, Madison WI 53706. ENT. NEWS 109(3) 189-190, May & June, 1998 1 90 ENTOMOLOGICAL NEWS roe County at Fort McCoy State Natural Area in 1995 and 1996. Six species (Table 1) are unlisted for Wisconsin and one of these, Dyschirius sextoni Bousquet, heretofore unrecorded for the United States (Bousquet and Larochelle 1993), is a rare clivinine described only recently (Bousquet 1987) that until now has been known only from the type locality in Belleville, Ontario, about 1000 km east of Fort McCoy. The four specimens we found were taken on 16 July 1996 by pan trap at a sand quarry in oak savanna. The five other ground beetles we report as new for Wisconsin (Table 1 ) are known from various adjoining states and Canada. They are species whose known habitat preferences are reflected in the sandy scrub biotope where they were taken, characterizing much of the Fort McCoy area. Table 1. Ground beetles new to Wisconsin, collected in Monroe County on the Fort McCoy Military Reservation (1995 and 1996). Dyschirius sextoni is also new to the United States. Clivinini Trap Date Dyschirius sextoni Bousquet 16Jul 1996 Harpalini Harpalus indianus Csiki 24Aug 1996 Harpalus indigens Casey 20 Jul 1 995 Sele nophorus planipennis LeConte 19Jun 1996 Lebiini Cymindis planipennis LeConte 25 Aug 1 996 Helluonini Helluomorphoides praeustus bicolor (Harris) 13 Jun 1996 ACKNOWLEDGMENTS We thank Robert L. Davidson, Carnegie Museum of Natural History, Pittsburgh for his gen- erous help with ground beetle determinations. The effort of Tim Wilder (Endangered Species Section, Environment and Natural Resources Management Division, Fort McCoy Military Res- ervation) in monitoring traps and curating insects is greatly appreciated. LITERATURE CITED Barndt, W.D. and J.E. Langton. 1 984. The soil survey of Monroe County, Wisconsin. U.S.D.A. Soil Conservation Service, Res. Div. Coll. of Agric. and Life Sci., U. Wise., Madison; 206 pp. Bousquet, Y. 1987. The carabid fauna of Canada and Alaska: range extensions, additions and descriptions of two new species of Dyschirius (Coleoptera: Carabidae). Coleopt. Bull. 41 (2): 11 1-135. Bousquet, Y. and A. Larochelle. 1993. Catalogue of the Geadephaga (Coleoptera: Trachy- pachidae, Rhysodidae, Carabidae including Cicindelini) of America north of Mexico. Mem. Entomol. Soc. Canada, No. 167. Curtis, J.T. 1959. The vegetation of Wisconsin: an ordination of plant communities. U. Wise. Press, Madison; 657 pp. Purrington, F.F. and K.J. Larsen. 1997. Records of thirteen ground beetles (Coleoptera: Carabidae) new to Iowa. J. Iowa Acad. Sci. 104(2): 50-51. Vol. 109, No. 3, May & June, 1998 191 TWO NEW SPECIES OF HYNESIONELLA (HETEROPTERA: GERRIDAE) FROM SOUTH AFRICA1 John T. Polhemus2 ABSTRACT: Two new species of Hynesionella, H. karatara, and H. slateri, are described from South Africa. The following new species of Trepobatinae are described to make the names available for completion of a world overview and checklist of the subfamily, and a key to the known species of Hynesionella Poisson is provided. A rede- scription of the genus Hynesionella was given by Polhemus & Polhemus ( 1 994), thus is not repeated here. In that work, the two species described below were listed as "two undescribed species from South Africa." All measurements are in millimeters. Hynesionella differs from the other two genera of Naboandelini, Naboandelus Distant and Calyptobates J. Polhemus & D. Polhemus, in having the posterior margin of the mesonotum definitely carinate medially as well as laterally, the metanotum strongly declivant, the mesonotum excavated behind posterolateral margins in both sexes, the posterolateral mesonotal plates large, almost vertical. Hynesionella also differs in having the male fore femur thick- ened, often distinctly bent, sculptured basally or medially, often with large ventral protuberances, whereas in Naboandelus the male fore femur slightly thickened with at most a slight basal protuberance set with very short setae, and in Calyptobates the fore femur is slender, very slightly thickened basally and unmodified. In most species sexual size dimorphism is pronounced, the males much smaller. The metasternal scent gland orifice (omphalium) is vesti- gial or absent, whereas it is present in the other two genera of Naboandelini, although very poorly developed. Hynesionella karatara NEW SPECIES Figures 1 - 3 Length, apterous male 2.63 (mean, N - 7; min. 2.55, max. 2.77), maximum width 1.30 (mean, N =• 7; min. 1 .28, max. 1 .39). Length, apterous female 3. 1 4 (mean, N - 3; min. 3.05, max. 3.27), maximum width 1.83 (mean, N - 3; min. 1.72, max. 1.89). General color black, sides covered with grayish pruinosity, appearing yellowish in certain light. Small elongate spot medially on pronotum brownish yellow. U-shaped marking on base of head extending forward along inner eye margins, brown. Median quadrate region of mesonotum glabrous; entire body set with short pubescence. 1 Received July 28, 1997. Accepted September 5, 1997. 2 Colorado Entomological Museum, 31 15 S. York St., Englewood, CO. 80110 ENT. NEWS 109(3) 191-194, May & June, 1998 1 92 ENTOMOLOGICAL NEWS Structural characters. Apterous male. Head set with moderate length black setae, length 0.44, width 0.89; eye width (0.22), about half the width of the interocular space (0.43). Pronotum short, lateral margins rounded, length 0.33, width 0.86; mesonotum long, broad, sides almost straight, posterior margin almost straight, length 0.78, width 1 .33 (Fig. 1 ); pronotum, mesonotum set with numerous black stout moderate length setae dorsally and laterally; metanotum fused with first two abdominal tergites, all three moderately long, weakly indicated laterally, combined length 0.50; abdominal tergites III-VI subequal in length (0.08-0.10), VII longer (0.17). Length of antenna! segments I-IV: 0.89; 0.55; 0.33; 0.33; segment I long, weakly fusiform (Fig. 3). Fore femur curved basally, notched, set with a thick brush of very short setae over most of length, forming a pad on distal half (Fig. 2); fore tibia slightly curved, broader on distal half. Measurements of legs as follows: femur, tibia, tarsal 1 , tarsal 2 of fore leg, 1.17, 0.78, 0.06, 0.33; of middle leg, 2.36, 3.50, 1.11, 0.78; of hind leg (tarsal 1 and 2 fused), 2.66, 1 .03, 0.44. Lateral arms of proctiger not prominent, blunt distally, captured between broad lateral mar- gins of tergite VIII and stemite VIII. Apterous female. Structure, dorsal setae, and coloration mostly as in male, except larger and more robust, with unmodified fore legs; antennal segment I distinctly more slender than in male. Length of antennal segments I-IV: 0.72; 0.55; 0.39; 0.42. Middle femur broad on basal 2/3, flattened. Type material. Holotype, apterous male, South Africa, Cape Prov., Karatara Pass, CL 968, 22 Feb. 1979, J. T. Polhemus (USNM). Paratypes (all apterous), 2 males, 2 females, same data as holotype (JTPC); 4 males, 2 females, South Africa, Cape Prov., 15 mi. (24 km) NW of Knysna, Phantom Pass Rd., 10 Feb. 1968, R. T. Schuh, J. & S. Slater, M. Sweet (JTPC, AMNH). Etymology. The name karatara, a noun in apposition, refers to the type locality, Karatara Pass. Comparative notes. The lack of any gray pruinose markings on the mesonotal dorsum separates karatara from the four other known species of Hynesionella. The sculpturing of the fore femur of karatara (Fig. 2) also is diagnostic. Hynesionella karatara sp. n. is closest to H. capensis (Poisson), as both of these species have the median part of the mesonotum shining black; in capensis, however, the median black area is narrow, flanked by gray pruinose, whereas in karatara it is broad, without gray pruinose. In addition, capensis is unique in having the posterior margin of the female mesonotum curved and extended posteriorly, instead of straight as in all other species of the genus. Hynesionella slateri, NEW SPECIES Figures 4-5 Length, apterous male 2.42 (mean, N = 3; min. 2.39, max. 2.44), maximum width 1.28 (mean, N = 3; min. 1 .28, max. 1 .28). Length, apterous female 2.89 (mean, N = 2; min. 2.89, max. 2.89), maximum width 1.58 (mean, N = 2; min. 1.55, max. 1.61). General color gray, sides covered with grayish pruinosity, appearing yellowish in certain light. Broad depressed quadrate areas on pronotum and mesonotum densely clothed with decum- bent, closely appressed whitish gray coarse pubescence underlain with whitish integument, at least on pronotum. Head orange yellow to orange brown, with a dark median wedge on anterior 1/2 to 2/3. Entire body set with short gray pubescence, longer on abdominal tergites. Fore femur orange except extreme base and extreme tip. Vol. 109, No. 3, May & June, 1998 193 Structural characters. Apterous male. Head with moderate length black setae, length 0.33, width 0.83; eye width (0.22), about half the width of interocular space (0.39). Pronotum short, lateral margins rounded, length 0.28, width 0.78; mesonotum long, broad, sides slightly curved, length 0.72, width 1 .27; pronotum, mesonotum with numerous black stout moderate length setae laterally and on pleura; metanotum fused with first two abdominal tergites, all three moderately long, weakly indicated laterally, combined length 0.39; abdominal tergites III-VI subequal in length (0.06-0.10), VII longer (0.14). Length of antennal segments I-IV: 0.68; 0.44; 0.33; 0.33; segment I long, very weakly fusiform (Fig. 4). Fore femur stout, short, curved basally, set with a thick brush of very short setae over most of length, forming a pad on distal half (fig. 5); fore tibia slightly curved, broader on distal half. Measurements of legs as follows: femur, tibia, tarsal 1 , tarsal 2 of fore leg, 0.89, 0.67, 0.06, 0.33; of middle leg, 2.77, 3.11,1.17, 0.72; of hind leg (tarsal 1 and 2 fused), 2.39, 0.94, 0.42. Lateral arms of proctiger not prominent, tapered distally, captured between broad lateral margins of tergite VIII and stemite VIII. Apterous female. Structure, dorsal setae, and coloration mostly as in male, except larger and more robust, median depressed area of mesontotum not clearly differentiated in color, dorsal pubescence shorter; fore legs unmodified; antennal segment I about as thick as in male. Length of antennal segments I-IV: 0.67; 0.39; 0.33; 0.33. Middle femur not broadened nor flattened. Type material. Holotype, apterous male, South Africa, Cape Prov., 18 mi. (28 km) NW of Kimberley, 18 Jan. 1968, R. T. Schuh, J. & S. Slater, M. Sweet (AMNH). Paratypes (all apter- ous), 2 males, 2 females, same data as holotype (JTPC, AMNH). Etymology. The name slateri honors James A. Slater in recognition of his outstanding and voluminous contributions to the study of Heteroptera. Comparative notes. Hynesionella slateri sp. n. is closest to H. aethiopica Hoberlandt, but differs in having the fore femur orange with a less pronounced basal notch. Figures 1 - 3. Hynesionella karatara sp. n. 1 . Female mesonotum, dorsal view. 2. Male fore leg. 3. Antenna. Figures 4 - 5. Hynesionella slateri sp. n. 4. Antenna. 5. Male fore leg. Scale bar = 1 mm. 194 ENTOMOLOGICAL NEWS Key to the known species of Hynesionella 1 . Mesonotal dorsum without gray pruinose markings karatara sp. n. Mesonotal dorsum with gray pruinose markings , 2 2. Fore femur with a prominent basal tubercle in both male and female . . . cobbeni Linnavuori — Male fore femur modified, but without a prominent basal tubercle; female fore femur unmodified 3 3. Fore femur mostly orange in both male and female slateri sp. n. Fore femur mostly dark colored, not orange 4 4. Male fore femur not excavated basally, not abruptly widened near middle; female mesonotum with posterior margin curved posteriorly capensis (Poisson) Male fore femur excavated basally, abruptly widened near middle; female mesonotum with posterior margin almost straight aethiopica Poisson ACKNOWLEDGMENTS I am indebted to R. T. Schuh and D. A. Polhemus for their constructive reviews. The invaluable assistance of Brian Stuckenberg and Dennis Brothers, Pietermaritzburg, South Africa, is gratefully acknowledged. Both aided with guidance and logistics, and without their help the field survey would not have been nearly as successful. J. A. Slater and R. T. Schuh kindly permitted me to study specimens under their care; the specimens, originally at the University of Connecticut, Storrs, have since been transferred to the American Museum of Natural History, New York. This research was sponsored by grant 1 806-77 from the National Geographic Society, Wash- ington, D. C., and by grant DEB-9528025 from the National Science Foundation, Washington, D. C. I thank these organizations for their continued support of research into the systematics and zoogeography of aquatic Heteroptera. This research was carried out as a faculty affiliate of the Entomology Department, Colorado State University, Fort Collins. LITERATURE CITED Polhemus, J. T. and D. A. Polhemus. 1994. The Trepobatinae (Gerridae) of New Guinea and surrounding regions, with a review of the world fauna. Part 2. Tribe Naboandelini. Entomol. Scand. 25: 333-359. BOOK REVIEW (concluded from page 188) Davies, D. A. L., & P. Tobin. 1984. The Dragonflies of the World: A Systematic List of the Extant Species of Odonata, Vol. 1 , Zygoptera, Anisozygoptera. Utrecht, S.I.O. Rapid Comm. (Suppl.)3. 127pp. Davies, D. A. L., & P. Tobin. 1985. The Dragonflies of the World: A Systematic List of the Extant Species of Odonata, Vol. 2, Anisoptera. Utrecht, S.I.O. Rapid Comm. (Suppl.) 5. 151 pp. I ohmunn, H. 1992. Revision der Cordulegastridae. 1. Entwurf einer neuen Klassifizierung der Familie (Odonata: Anisoptera). Opusc. Zool. Flumin. 96: 1-18. Tsuda, S. 1991 . A Distributional List of World Odonata. Osaka, privately published. 362 pp. - Michael L. May Department of Entomology Rutgers University, NJ Vol. 109, No. 3, May & June, 1998 195 REASSIGNMENT OF ISOTOMA LOUISIANA (COLLEMBOLA: ISOTOMIDAE)1 R.D. Waltz2 ABSTRACT: The holotype specimen of Isotoma louisiana was examined and found to be as- signable to the genus Isotomurus. The species is distinct among all Nearctic species based on the tuberculate denies, mucro with a unique ventral tooth, and a unique chaetotaxy. Comparisons and contrasts with other selected isotomid genera are made justifying its inclusion in Isotomurus. The cosmopolitan hydrophilic genus Isotomurus Borner is readily distin- guished among other Isotomidae by the combination of a quadridentate mu- cro, presence of long, specialized sensory setae known as bothriotricha, greater than ten ventral manubrial setae, and a characteristic maxillary outer lobe. The genus in North America is comprised of eight nominal species (Christiansen and Bellinger 1980), including this present species, and several species to be described in forthcoming work. In preparation for a review of Nearctic Iso- tomurus, I reviewed the holotype of Isotoma louisiana Scott and found it to be assignable to Isotomurus. The species is herein redescribed and newly com- bined as Isotomurus louisiana (Scott), n.comb. Some illustrations of the spe- cies were provided by Scott (1962); further comparative illustrations will be presented in the forthcoming revision of the Collembola of North America, north of the Rio Grande, by Christiansen and Bellinger (in press) and a review of Nearctic Isotomurus species (Waltz, MS). This species is unique among all Nearctic species based on its possession of tuberculate dentes and a strongly excavate ventrobasal projection ("ventral tooth" ) of the mucro. Isotomurus louisiana (Scott), NEW COMBINATION Description: Yellow or green body color in most specimens, with or without strongly contrast- ing color patterns. Color pattern, when present, with medial and lateral longitudinal stripes and with or without banded posterior abdominal terga. Prominently patterned individuals may also possess a distinct ventral stripe medially on the ventral abdominal segments extending to Th II. The holotype specimen possesses purple stripes, posterior abdominal bands and a ventral purple stripe medially on a yellow body. Specimens studied from Arizona are light green and without contrasting pattern. Specimens studied from Kansas are very similar to the type material but lack the ventral purple stripe. Head: PAO less than or subequal to nearest eye. Eyes G and H smaller than remaining eyes. Outer lobe of maxilla with palp bifurcate and with four sublobal setae. Thorax: Unguiculus without inner tooth. Unguis elongate, without teeth. Tibiotarsi of metatho- racic legs without long, outstanding, exterior setae. 1 Received October 2, 1997. Accepted October 15, 1997. 2 IDNR, Division of Entomology and Plant Pathology, 402 West Washington, Room W-290, Indianapolis, IN 46204. ENT. NEWS 109(3) 195-197, May & June, 1998 196 ENTOMOLOGICAL NEWS Abdomen: ABD. V-VI without coarse ciliate macrochaetae; common body setae numerous and non-ciliate; bothriotricha of Abd. II-Abd. IV as 3+3+1 pairs; Abd. V S setae (terminology after Deharveng and Lek 1993): accp I is absent, accp 2, accp 3, accp 4, and accp 5 present, as 1 and as 2 are present and a third seta here designated as as X is present anterior to as 1 and 2; ventral tube with 12-22 lateral distal setae; tenaculum quadridentate (not as illustrated by Scott 1962: Fig. 2) with 12-25 setae; denies tuberculate; mucro with mucronal basal seta and lamella, ventral mucronal tooth present, i.e., with excavate ventromucronal base. Known distribution: Arizona, Kansas, Louisiana. Material examined: (Academy of Natural Sciences, Philadelphia) Holotype (#107), on slide, "taken on water, shore of Lake Pontchartrain, approximately 1 5 feet above mean sea level, Norco, St. Charles Parish, Louisiana, 29-iii-1960, J.H. Eslinger." Label affixed ventrally: Isotomurus louisiana (Scott) n. comb. Del: R.D. Waltz 5/97. Additional material examined (Presently in the collection of K.A. Christiansen): AZ: NE of Parker, puddle at edge of Colorado River, 28-x- 1986, P. Bellinger (7051). AZ: Pima Co., Santa Rita Mtns, Florida Saddle, SE Tuscon, 7-iii- 1989, Olson (7193). KS: Leavenworth Co., 1 mile N Eudora, Kansas River, 8-ix-1988, (52-1- 7,12), D.S. Hammer and L.C. Ferrington. KS: Douglas Co., (7072). Remarks: This species, first described by Scott (1962), was deposited at the Collection of the Academy of Natural Sciences of Philadelphia (Scott 1962; Roback 1981). Bellinger (1985) reviewed the holotype specimen and reported the species as similar to "Agrenia (tuberculate denies) and Isotomurus (abdominal bothriotricha)" but commented that it did not fit into any known genus. The location of the remainder of the paratype material cited by Scott (1962) is unknown. This review of this "striking species" (Bellinger 1985) has resulted in reas- signment of this species within Isotomurus rather than Agrenia Borner or an- other genus due to the following symmorphic characters shared with Isotomurus species: 1 ) maxillary outer lobe possesses a bifurcate palp and four sublobal hairs (versus bifurcate palp and no sublobal hairs in Agrenia) (see Fjellberg' 1984); 2) denies lack the distal elongate setae found in all Agrenia species (see Fjellberg 1986, 1988); 3) presence of abdominal bothriotricha (found also in Hydroisotoma Stach, Archisotoma Linnaniemi, and other Isotomidae but not reported in Agrenia); 4) the characteristic Isotomurus quadridentate mucro (quite distinct from the mucro of Agrenia, Archisotoma, and Hydroisotoma); 5) claws that lack the distinct tunica of the ungues (present only in Agrenia); and 6) a manubrium with many ventral setae (few only in Archisotoma). That /. louisiana belongs in Isotomurus is strongly indicated by the above charac- ters which are uniquely symmorphic with Isotomurus and not shared in com- bination with other known genera characterized by the possession of abdomi- nal bothriotricha (especially Archisotoma and Hydroisotoma) or with tubercu- late denies (Agrenia). As noted by Bellinger (op cit) Isotomurus louisiana dif- fers from other previously described species in the genus by the luberculale denies (mosl Isotomurus species bear crenulale denies; some bear only par- lially luberculate denies). Vol. 109, No. 3, May & June, 1998 197 The combination of tuberculate denies, the excavate ventral base of the mucro, and the Abd. V chaetotaxy is unique among European (see Deharveng and Lek 1993) and Nearctic Isotomurus species and provides the basis for diagnosis of this species. Partially tuberculate dentes have been reported in at least one Palearctic species, /. ciliatus Stach. Several new species are pending description in the Nearctic including species with fully tuberculate dentes and partially tubercu- late dentes. Differences in dental structure have been found to be useful historically in justifying generic or subgeneric status (e.g., subgenera ofProisotoma Borner). For the present, elevation of this species and putatively related species bearing tuberculate or partially tuberculate dentes to a subgenus grouping appears to be clearly countered by the existence of intermediate dental types, and an ab- sence of clearly autapomorphic chaetotactic states or other morphology rela- tive to the type species of the genus, /. palustris (Muller). ACKNOWLEDGMENTS I thank D. Azuma, Academy of Natural Sciences, Philadelphia, for loan of the Holotype slide of/. Louisiana. I thank P. Bellinger and K.A. Christiansen for loan of Nearctic collections of this species and preliminary data and Arwin Provonsha, Purdue University, for providing helpful comments on an early draft of this manuscript. LITERATURE CITED Bellinger, P.P. 1985. The identity of H.G. Scott's Collembola in the Academy of Natural Sci- ences, Philadelphia, PA. Entomol. News 96: 78-82. Christiansen, K.A. and P. F. Bellinger. 1980. The Collembola of North America, north of the Rio Grande. Part 2. Families Onychiuridae and Isotomidae. Grinnell College, Grinnell, Iowa, pp. 387-784. Deharveng, L. and S. Lek. 1993. Remarques sur la morphologic et la taxonomie du genre Isotomurus Borner, 1903 et description de deux especes nouvelles de France (Collembola: Isotomidae). Ann. Entomol. Soc. France (N.S.) 29: 245-259. Fjellberg, A. 1984. The maxillary outer lobe, an important systematic tool in Isotomidae (Collembola). Annls. Soc. royale Zool. Belg. 114: 83-88. Fjellberg, A. 1986. Revision of the genus Agrenia Borner, 1906 (Collembola, Isotomidae). Entomol. Scand. 17: 93-106. Fjellberg, A. \98S.Agrenia lamellosa, a new species of Collembola (Isotomidae) from Pennsyl- vania. J. N. Y. Entomol. Soc. 96: 110-112. Roback, S.S. 1981. Collembola described by Dr. H.G. Scott in the collections of the Academy of Natural Sciences of Philadelphia. Entomol. News 92: 209-210. Scott, H.G. 1962. Collembola from Louisiana. Entomol. News 73: 27-28. 198 ENTOMOLOGICAL NEWS A NEW HOST RECORD FOR SPHAEROPTHALMA PENSYLVANICA PENSYLVANICA (HYMENOPTERA: MUTILLIDAE)1'2 Donald G. Manley,3 T. Paige Carithers4 ABSTRACT: Wasps belonging to the family Mutillidae are known to be parasitic on the resting stages of other insects, including other Hymenoptera. However, host records are known for less than five percent of the described species. The mutillid Sphae ropthalma pensylvanica pensylvanica has been reported emerging from at least two different species and genera of sphecid hosts (Hymenoptera: Sphecidae). Here we report the emergence of S. pensylvanica from yet another species and genus of sphecid host, that being Isodontia mexicana. Details are described. Sphaeropthalma (Sphaeropthalma) pensylvanica pensylvanica (Hymenop- tera: Mutillidae) was first described as Mutilla pensylvanica by Lepeletier (1 845), based on a male collected in Philadelphia. It is found from North Caro- lina to central Florida, Louisiana, Missouri, Kansas and Texas. A second sub- species, 5. pensylvanica scaeva, was described as M. scaeva by Blake (1871). The female was described in the same publication asM. balteola (Blake 1871). It is found from North Carolina to Massachusetts, Ohio, Illinois, Missouri, Kansas and Texas. A third subspecies, Sphae rophthalma (!) pennsylvanica (!) floridensis, was described by Schuster (1944). It is found only in southern Florida. The females of all three subspecies are indistinguishable. Members of the family Mutillidae are known to be parasitic on the resting stages (usually the prepupa or pupa) of other insects, including aculeate Hy- menoptera. Of more than 4000 described species of Mutillidae, host records exist for less than five percent (Brothers 1972). Sphaeropthalma pensylvanica is one of the species for which at least some hosts are known. Rau (1922) reported a male specimen of S. pensylvanica scaeva emerging from a cocoon of the mud dauber Sceliphron caementarium (Drury) (Hy- menoptera: Sphecidae) in Missouri. He later (Rau 1928) reported males of 5. pensylvanica scaeva having been reared from the cocoons of both Trypoxylon (Trypargilum) politum Say (Hymenoptera: Sphecidae) and from Sceliphron nests. In 1984, a male mutillid was sent to Manley for identification. The mutillid 1 Received September 29, 1997. Accepted October 20, 1997. 2 Technical contribution no. 4344 of the South Carolina Agricultural Experiment Station, Clem- son University. 3 Department of Entomology, Clemson University, Pee Dee Research and Education Center, 2200 Pocket Rd., Florence, SC 29506-9706. 4 Department of Zoology and Wildlife Science, 101 Cary Hall, Auburn University, Auburn, AL 36849. ENT NEWS 109(3) 198-200, May & June, 1998 Vol. 109, No. 3, May & June, 1998 199 was determined to be S. pensylvanica pensylvanica. It had emerged from the cocoon of a mud dauber (the host cocoon was not sent for determination) on 30 May 1984. The cocoon was collected by J. R. Brushwein in Clemson, South Carolina in March of 1984. Here, we report the emergence of a male S. pensylvanica pensylvanica from a diapaused cocoon of another sphecid wasp, Isodontia mexicana (Saussure). This North American wasp ranges throughout the United States east of the Rocky Mountains, south into Mexico and Central America, and has also become established in Hawaii and France (Bohart & Menke 1 963). Across this wide range, this species has been observed to nest in such above-ground cavities as hollow plant stems and twigs, rolled leaves, abandoned bee bur- rows in logs, artificial trap-nests, and the tubular leaves of pitcher plants (Sar- racenia) (Bequaert 1930). Isodontia mexicana is one of the "grass carrier (or carrying) wasps" because the females are often seen carrying blades of grass in their mandibles (Bohart & Menke 1976). The females will then proceed to use these plant fibers to construct their nests. Nesting by /. mexicana in the leaves of pitcher plants has been well docu- mented (under synonym Isodontia philadelphica St. Farg.) (Hubbard 1 896, Jones 1904, Fish 1976, Rymal & Folkerts 1982). Over 400 Isodontia nests in pitcher leaves were reared for a study on the nesting biology of this wasp (Carithers 1998). The male mutillid reported here was reared from an /. mexicana nest built in the tubular leaf of the pitcher plant Sarraceniaflava. The nest was collected on 9 November 1996 in the Apalachicola National For- est in Liberty County, Florida. One intact Isodontia cocoon was contained in the one-celled nest. Upon holding the cocoon up to a light source, it appeared to contain a flacid Isodontia pre-pupa. A mutillid male emerged from the Isodontia cocoon in an environmental chamber on 13 March 1997. Within the Isodontia cocoon, the mutillid had spun its own cocoon. This is believed to be the first report of a mutillid parasite from /. mexicana, although Bohart and Menke (1976) reported that Sphaeropthalma has been bred from the nest of/. elegans (F. Smith). LITERATURE CITED Bequaert, J. 1930. Nesting habits of Isodontia, a subgenus of Chlorion (Hymenoptera). Bull. Brooklyn Entomol. Soc. 25: 122-3. Blake, C. A. 1 87 1 . Synopsis of the Mutillidae of North America. Trans. Amer. Entomol. Soc. 3: 217-265. Bohart, R. M. and A. S. Menke. 1963. A reclassification of the Sphecinae with a revision of the nearctic species of the tribes Sceliphronini and Sphecini. Univ. Calif. Pub. Entomol. 30: 90- 182. Bohart, R. M. and A. S. Menke. 1976. Sphecid Wasps of the World. Univ. Calif. Press, Berke- ley, Calif. 695 pp. Brothers, D. J. 1972. Biology and immature stages of Pseudomethocaf. frigida, with notes on other species (Hymenoptera: Mutillidae). Univ. Kans. Sci. Bull. 50: 1-38. 200 ENTOMOLOGICAL NEWS Carithers, T. P. 1 998. Nesting biology of the grass-carrier wasp Isodontia mexicana (Sphecidae) in pitcher plants (Sarracenia). M. S. thesis, Auburn University, Auburn, AL. Fish, D. 1976. Insect-plant relationships of the insectivorous pitcher plant Sarracenia minor. Fla. Entomol. 59: 199-203. Hubbard, H. G. 1 896. Some insects which brave the dangers of the pitcher plant. Proc. Entomol. Soc. Wash. 3: 314-318. Jones, F. M. 1904. Pitcher-plant insects. Entomol. News 15: 14-19. Lepeletier, A. L. M. 1845. Histoire Naturelle des Insectes. Hymenopteres. Paris. 1: 14; 3: 516- 517,588-646. Rau, P. 1 922. Ecological and behavior notes on Missouri insects. Trans. Acad. Sci. St. Louis 24: 3-8. Rau, P. 1928. Field studies in the behavior of the non-social wasps. Trans. Acad. Sci. St. Louis 25: 325-489. Rymal, D. E., and G. W. Folkerts. 1982. Insects associated with pitcher plants (Sarracenia: Sarraceniaceae), and their relationship to pitcher plant conservation: a review. J. Ala. Acad. Sci. 53: 131-151. Schuster, R. M. 1944. Notes and records of the Eastern representatives of the Photopsidine genera of Mutillidae with descriptions of new forms. Bull. Brooklyn Entomol. Soc. 39: 139- 155. ANNOUNCEMENT 1999-2000 FULBRIGHT AWARDS FOR U.S. FACULTY AND PROFESSIONALS Opportunities for lecturing or advanced research in over 125 countries are available to col- lege and university faculty and professionals outside academe. U.S. citizenship and the Ph.D. or comparable professional qualifications required. For lecturing awards, university or college teach- ing experience is expected. Foreign language skills are needed for some countries, but most lecturing assignments are in English. Deadlines: •August 1, 1998, for lecturing and research grants in academic year 1999-2000 • May 1, 1998, for distinguished Fulbright chairs in Western Europe and Canada • November 1, 1998, for international education and academic administrator seminars Contact the USIA Fulbright Senior Scholar Program, Council for International Exchange of Scholars, 3007 Tilden Street, NW, Suite SL, Box GNEWS, Washington, DC 20008-3009. Tele- phone: 202-686-7877. Web page (on-line materials): http://www.cies.org. E-mail: apprequest(2i).cies. iie.org (requests for application materials only). Vol. 109, No. 3, May & June, 1998 201 ODONATA OF THE SOUTH CENTRAL NEARCTIC REGION, INCLUDING NORTHEASTERN MEXICO1 John C. Abbott, Kenneth W. Stewart2 ABSTRACT: There has not been a concerted effort to document the extent of biodiversity, distri- bution and geographic affinities of the Odonata of the south central United States and northeast- ern Mexico. The area is an important boundary for some species representing eastern Nearctic and subtropical faunas, and a mixing zone or dispersal corridor for other species. Since 1993, we have done extensive collecting of all life stages in the seven biotic provinces of Texas, and com- piled published and extensive unpublished records from the portions of these provinces of U.S. and Mexican states that join Texas. Here we list 228 species for this region (196 in Texas), indi- cate their distributions by biotic province, and discuss the regional biogeography and importance of rare species. Current emphasis on inventorying aquatic insects of the neotropics Paulson 1982; (Gonzalez and Novelo 1991; Novelo and Gonzalez 1991; Quintero and Aiello 1992; Baumann and Kondratieff 1996; Gonzalez and Novelo 1996; McCafferty & Lugo-Ortiz 1996) and realization of the great risk to aquatic invertebrate biodiversity in temperate regions (Franklin 1988; Haffernik 1989, 1992), prompted us to investigate the status of the odonate fauna of the biotic provinces of Texas and adjoining states of the United States and northeastern Mexico that border the Rio Grande River. The odonate fauna of this region is poorly known except from scattered publication records, un- published reports of collectors, the general documentation and discussion of Mexico's fauna by Gonzalez and Novelo (1996) and Johnson's (1972) work on Texas Zygoptera. There has never been a concerted effort to document the biodiversity and geographic affinities of the Odonata of this area. Over half of the species of Odonata still unknown as larvae are from this region (McCafferty etal. 1990). The earliest major documentation of Odonata in Texas was by Hagen ( 1 861 ). A number of species accounts from the state then appeared in Calvert (1901- 1 908) and Muttkowski (1910). Williamson (1914) listed numerous records for Texas and Oklahoma. Several localized studies within Texas have supplemented these works: Tinkham (1934) and Gloyd (1958) from West Texas and Tucker (1908), Ferguson (1940, 1942), Harwell (1951), Donnelly (1978), Williams (1982), and Laswell and Mitchell (1997) from north central and eastern parts of the state. Abbott (1996) reported the following new species records for Texas: Aeshnapsilus Calvert, Gomphus exilis (Selys), Somatochlora georgiana Walker, Dythemis maya Calvert, Micrathyria didyma (Selys), Sympetrum illotum 1 Received July 18, 1997, Accepted September 20, 1997. 2 Department of Biological Sciences, University of North Texas, Denton, Texas 76203 USA. ENT. NEWS 109(3) 201-212 , May & June, 1998 202 ENTOMOLOGICAL NEWS (Hagen), Tauriphilia azteca Calvert, and Tramea insularis Hagen. Johnson's (1972) treatise on Texas Zygoptera remains the only comprehensive faunal analysis of that group for the state. Young and Bayer (1979) compiled a list of the dragonfly larvae in the Guadalupe River basin in Texas. Only Kennedy (1921) has made a major effort to describe larvae of several species in the region. Species lists have been published for the peripheral states of Louisiana (101 spp.; Bick 1957), Arkansas (133 spp.; Harp and Rickett 1977; Harp 1983a,b; Harp 1985; Harp and Harp 1996), Oklahoma (126 spp.; Bick and Bick 1957) and New Mexico (97 spp.; Evans 1995). These lists have been helpful additions to the knowledge of dragonfly and damselfly distributions in this region, but generally have not involved the systematic sampling of stream systems or vegetational or physiographic subregions (except Bick 1957), nor association with abiotic and biotic parameters of occurrence, necessary for biogeographic analysis. STUDY AREA AND METHODS The south central Nearctic Region, as we are defining it (Fig. 1), covers approximately 560,000 km2, of which 412,000 km2 are in Texas. It includes the seven biotic provinces of Texas and the portions of those provinces from Arkansas, Oklahoma, Louisiana, New Mexico and northeastern Mexico that immediately join Texas. The Mississippi River forms the eastern boundary, and the Navahonian biotic province bounds the western edge of the region. Mean annual precipitation ranges from 25-147 cm/yr, most falling in March- May. Major vegetation types include eastern pines and hardwoods, central prai- ries and grasslands and western deserts. The seven distinct regional biotic prov- inces differ in topography, temperature, vegetation, soil type, geology and cli- mate as outlined by Blair (1950), Dice (1943) and Blair and Hubbell (1938). They are: 1) Chihuahuan, 2) Navahonian, 3) Kansan, 4) Balconian, 5) Tamaulipan, 6) Texan, and 7) Austroriparian (Fig. 1). Elevation ranges from sea level to 2,667 meters (Guadalupe Peak, Culberson Co., TX) in the Guadalupe Mountains National Park. Intensive sampling, with emphasis on Texas, began in September, 1993 and included more than 30 expeditions transecting the biotic provinces or con- centrating in such areas as the Guadalupe Mountains National Park of the Navahonian Province. Collection of adults and larvae involved sampling all traversed lotic habitats and selected lentic habitats. Documentation of the fauna of states adjacent to Texas was based primarily on both published and unpub- lished records of acknowledged collectors and museum holdings, and limited sampling. Adults were placed in glassine envelopes and submerged in 99% acetone for overnight, then removed and allowed to dry completely before being per- Vol. 109, No. 3, May & June, 1998 203 Oklahoma NAVAHONIAN BALCONIAN f,'~ AMAULIPA Mexico Figure 1. The six natural biotic provinces of the south central Nearctic Region (modified from Blair 1950, Dice 1943 and Blair and Hubbell 1938). manently stored in polyethylene envelopes with data cards. Larvae were col- lected using a Wildco-type 46 \ 22.5 cm net with a 1 mm mesh size. Exuviae were hand collected from emergence sites. Both exuviae and larvae were placed in Kahle's solution and then rinsed and permanently preserved in 80% ethanol upon returning to the laboratory. Numerous distribution records were based on reared species; mature larvae were transported alive to the laboratory in por- table styrofoam rearing chambers as described by Szczytko and Stewart ( 1 979), and reared in a conditioned Frigid Units Living Stream™ maintained at collec- tion or slightly raised temperatures. Collections Examined We examined all regional material from the extensive and previously undocumented Beatty collection, housed at the Frost Entomological Museum of Penn State University (PSU) and from 204 ENTOMOLOGICAL NEWS the Arkansas State University Museum of Zoology (ASUMZ), Florida State Collection of Arthropods (FSCA), International Odonata Research Institute (IORI), Sul Ross State University (SRSU) and the Texas A&M Insect Collection (TAMU). Individuals and institutions who do- nated study material include T.W. Donnelly (Binghamton, New York), S.W. Dunkle (Collin County Community College), J. Gelhaus (Academy of Natural Sciences of Philadelphia), S. Jasper (Texas A&M University), B.C. Kondratieff (Colorado State University), D.R. Paulson (University of Puget Sound) and C.R. Nelson (University of Texas, Austin). RESULTS Table 1 lists the 228 species of damselflies and dragonflies currently known from the south central Nearctic biotic provinces (Fig. 1). The classification used here follows that of Garrison (1991), except that in our view Epicordulia and Tetragoneuria are considered subgenera of Epitheca (K.J. Tennessen pers. comm.). The following four species represent new Texas species records, and one, Neoneura amelia, is a new U.S. species record: Neoneura amelia Calvert. TEXAS: Hidalgo Co., Rio Grande River nr. La Lomita Mission, W of Granjeno, 12 May 1997, 3Cf , 29, Univ. of North TX Collection. New for the US. N eoerythromma cultellatum (Hagen in Selys). TEXAS: Hidalgo Co., Rio Grande River nr. La Lomita Mission, W of Granjeno, 12 May 1997, 2Cf , Univ. of North TX Collection. New for Texas. Aphylla williamsoni (Gloyd). TEXAS: Jefferson Co., 1 2 August 1989, 1 Cf , photograph taken by R.A. Honig. TEXAS: Montgomery Co., pond @ E side of IH 45 and S of FM 1488, 11 August 1997, 1 9 , photograph taken by R.A. Behrstock. New forTX. Somatochlorafilosa (Hagen). TEXAS: Trinity Co., Rt. 94, 1 .3 mi W of Angelina County line, 23 August 1995, 1 9 , B. Mauffray Collection. New for TX. DISCUSSION The south central Nearctic Region (Fig. 1) is important as a boundary (Paulson 1982) for some species of the largely eastern fauna of central and east Texas (Texan and Austroriparian Provinces) that represent a temperate element, and those of south Texas and northeastern Mexico (Tamaulipan Prov- ince), representing a subtropical element. However, for other species these provinces are actually a mixing zone, and at least a short distance dispersal corridor; for example, our records indicate that of the 177 species occurring in the Austroriparian and Texan Provinces, 81 species (46%) also occur in the Tamaulipan Province and 59 species (33%) cross the Rio Grande to the south, into northeastern Mexico. Conversely, of the 108 species in the northeastern Mexico states of Tamaulipas and Nuevo Leon, 80 species (74%) cross the Rio Grande, entering Texas and 61 species (57%) occur in the Austroriparian and Texan Provinces. A similar boundary or mixing phenomenon exists near the Rio Grande River between the more northern Kansan/Navahonian/Balconian and the more southern Chihuahuan Province. Of the 142 species occurring in the Kansan, Vol. 109, No. 3, May & June, 1998 205 Navahonian and Balconian Provinces, 84 species (59%) also occur in the Chihuahuan Province and many of these cross the Rio Grande farther south, into northeastern Mexico. Conversely, of the 50 species in the northern Mexico states of Coahuila and Chihuahua, 41 species (82%) cross the Rio Grande, entering Texas with 39 (46%) of them occurring in the Kansan, Navahonian and Balconian Provinces. The Balconian Province represents diverse species assemblages from the northern and southern provinces bordering or near it. Fifty percent (99) of the 199 species occurring in the northern Navahonian/Kansan/Texan/Austroriparian Provinces occur in the southern Chihuahuan/Tamaulipan Provinces. Conversely, 93 (69%) of the 1 35 species occurring in the southern Chihuahuan/Tamaulipan Provinces occur in the northern Kansan/Texan/Austroriparian Provinces. These examples and recent discoveries of dispersals across the Rio Grande (Abbott 1996), including Neoneura amelia Calvert, Neoerythromma cultellatum Hagen in Selys, Aeshna psilus Calvert, Dythemis maya Calvert, Micrathyria didyma (Selys), Tauriphilia azteca Calvert and Tramea insularis Hagen, from Mexico into Texas suggest that the area is indeed a dispersal corridor and that future dispersals across it may occur. These distinct species assemblages and mixing zones undoubtedly relate to the climate and vegetational characteristics defin- ing these provinces. The Rio Grande border is a more effective barrier among certain groups than in others. The cordulegastrids and corduliids are poorly represented in Mexico, Central and South America, while groups such as the coenagrionids, aeshnids and libellulids are much more widely distributed and are well represented in these areas (Paulson 1982). The large number of Odonata larvae still unknown to science from the southwestern U.S. is attributed in part to relatively less collecting effort (McCafferty et al. 1990) and low species densities in combination with sparse and patchy habitats (Provonsha and McCafferty 1973). The larval descriptions of many of these regional species are insufficient for identification purposes (McCafferty et al. 1990). The rarity and/or local distributions of many species in the eastern part of this region account for the large number of unknown larvae there. Three species of Odonata in the south central Nearctic Region are listed as "species of concern" by the United States Fish and Wildlife Service. This fed- eral listing identifies species "for which information now in the possession of the Service indicates that proposing to list as endangered or threatened is pos- sibly appropriate, but for which persuasive data on biological vulnerability and threat are not currently available to support proposed rules" (USFWS 1 996). Two species, Somatochlora margarita Donnelly and Argia leonorae Garrison have locally restricted distributions. Argia leonorae is known from only five counties in south and west Texas and in northern Mexico. The larva of this species, in its natural habitat, remains undiscovered. 206 ENTOMOLOGICAL NEWS A status survey of Somatochlora margarita conducted by the Texas Parks and Wildlife Department (Price et al. 1989) expanded its initial range, a five mile radius in the Sam Houston National Forest, to a now estimated 10,000 square mile area in east Texas and western Louisiana. Somatochlora margarita is endemic to the longleaf and loblolly pine forests of southeastern Texas. We are in the process of describing its larva from the exuviae of reared material (donated by S.W. Dunkle). We believe that rigorous sampling in the sandy bottom streams of the Big Thicket of East Texas will lead to the discovery of this larva in its natural habitat, and provide potential insights into its life history. A third species, Macromia wabashensis Williamson, is also listed as Cat- egory 2 by the USFWS. Dubious records and the validity of M. wabashensis as a distinct species are factors leading to its Category 2 status. Several speci- mens referred to as M. wabashensis have been collected in McLennan and Falls Counties, Texas (Williams 1982). These records are currently thought to be exceptionally yellow forms of M. taeniolata Rambur or a hybrid between M. taeniolata and M. pacifica Hagen (Dunkle pers. comm.; Garrison 1995). It has thus been omitted from our list. There is considerable taxonomic confusion concerning the Tetragoneuria group of this region. We provisionally list Epitheca (T.) semiaquea (Burmeister) from Texas and Oklahoma based on determinations made by K.J. Tennessen. Further study including DNA analysis is needed on this group. Bick (1983) reported 32 species of North American Odonata (8% of that fauna) to be at risk, citing the loss of high-quality, undisturbed streams as the most significant factor endangering odonates in North America. Four of these (Neoneura aaroni Calvert, Gomphus ozarkensis Westfall, Somatochlora mar- garita Donnelly and 5. ozarkensis Bird) occur in the south central U.S. and are considered rare (Bick 1983). Effective conservation efforts depend on accu- rate knowledge of the current distribution of each species (Moore 1991). Continued effort is needed to indicate which strategies should be taken to conserve particular species and which breeding sites should be given priority for protection. ACKNOWLEDGMENTS We wish to thank all who provided data and support, especially S.W. Dunkle, T.W. Donnelly, R.W. Garrison, B. Mauffray and G.L. Harp. K.J. Tennessen provided valuable input and verifica- tion of specimens of Epitheca. We would like to thank S.R. Moulton, II and G.H. Beatty for allowing us to examine the Beatty collection, Frost Entomological Museum at Penn State. R.A. Behrstock and R.A. Honig generously furnished us with photographic records. Valuable field help was provided by J.W. Chirhart, J.O. Martinez, K.V. Moore and M.V. Passanante. We also thank S.W. Dunkle, C.R. Nelson and two anonymous reviewers for comments on earlier drafts of this manuscript. Vol. 109, No. 3, May & June, 1998 207 Table 1 . Distribution of the 227 species of Odonata currently known from the south central Nearctic Region. Arkansas (AR), Austroriparian (AUST), Balconian (BALC), Chihuahua (CHI), Chihuahuan (CHIH), Coahuila (COA), Kansan (KANS), Louisiana (LA), Navahonian (NAVA), New Mexico (NM), Nuevo Leon (NLN), Oklahoma (OK), Tamaulipan (TAMA), Tamaulipas (TAM), Texan (TEXA) and Texas (TX). Distributional annotations are noted by: (*) = new state record, (**) = new U.S. record. United States Mexico Biotic Provinces A L N O T R A M K X C C N T H O L A I A N M A B C K N T T U A H A A A E S L I N V M X T C H S A A A ZYGOPTERA (73) Calopterygidae (5) Calopteryx dimidiata Burmeister X X X C. maculate (Beauvois) XX XX XXX Hetaerina americana (Fabricius) X X X X X X X X X X X X X X X X H. litia (Drury) XX XX X X X X X X X X X H. vulneraia Hagen in Selys X X X X X XXX Lestidae (9) Archilestes grandis (Rambur) X XXX X XX X X X X X X Lestes alacer Hagen XXX XXX X X X X X X X L. disjunctus australis Walker X X X X X X X X X X X X L.forficula Rambur X X X XX L. inaequalis Walsh XX XX X L. rectangularis Say X X XXX L. sigma Calvert X X X X X XX L. unguiculatus Hagen X X XX X L. vigilax Hagen XX XX X Protoneuridae (3) Neoneura aaroni Calvert X X X X N. amelia Calven** * X Protoneura cara Calvert X X X X Coenagrionidae (56) Acanthagrion quadratum Selys X X X X X Amphiagrion abbreviatum (Selys) X X XXX Argia alberta Kennedy X X X X A. apicalis (Say) X X X X X X XXX XXX A. barretti Calvert X X X XX XX A. bipunctulata (Hagen) XX XX X X A. cuprea (Hagen) X X X X A.fumipennis Burmeister X X X X X X X X X X X X X A. hinei Kennedy X X X XXX A. immunda (Hagen) X XXX X XX X X X X XX A. leonorae Garrison X X XX XX A. lugens (Hagen) XXX X XX X A. moesta (Hagen) X X X X X X X X X X X X X X X X A. munda Calvert X X X X A. nahuana Calvert XXX X X X X X X X X X A. plana Calvert X XXX XXX X X X X X X X A. rhoadsi Calvert X X X X A. sedula (Hagen) X X X X X X X X X X X X X X X X A. tibialis (Rambur) XX XX XXX A. translata Hagen in Selys X XXX X XX X X X X X X X Chromagrion conditum (Selys) X X Enallagma antennatum (Say) X X £. aspersum (Hagen) X XX X X E. basidens Calvert X X X X X X XX X X X X X X X £. boreale Selys X XXX E. civile (Hagen) X X X X X X X X X X X X X X X X £. concisum Williamson X X £. cyathigerum (Charpentier) X XXX £. daeckii (Calvert) XX XX X £. divagans S61ys XX XX X X £. doubledayi (Selys) X X £. dubium Root X XX X 208 ENTOMOLOGICAL NEWS Table 1 (Continued) United States Mexico Biotic Provinces A L N O T R A M K X C C N T H O L A I A N M A B C K N T T U A H A A A E S L I N V M X T C H S A A A £. durum (Hagen) X X X X XX £. exsulans (Hagen) XX XX X X X X X X XX £. geminatum Kellicott XX XX X X E. ncvaehispaniae Calvert X X X XX X £. praevarum (Hagen) XXX XXX X X X X X £. signatum (Hagen) XX XX XX X XX £. traviatum westfalli Donnelly XX XX X X E. vesperum Calvert XX XX X X Hesperagrion heterodoxum (Selys) X X XXX X X X X Ischnura barberi Cmrie XXX X X X X X /. damula Calvert XXX XXX /. demorsa (Hagen) XXX X X XXX /. denticollis (Burmeister) XXX X XXX X /. hastata (Say) X X X X X X X X X X X X X X /. kellicotti Williamson XX XX X /. perparva McLachlan in Selys X X XXX /. posila posila (Hagen) XX XX X X X X XX /. prognata (Hagen) X X X /. ramburii (Selys) XX XX X XX X X X X XX /. verticalis (Say) X XXX X XXX Nehalennia integricollis Calvert X XX X Neoerythromma cultellatum (Selys) * X X Telebasis byersi Westfall XX X X T. salva (Hagen) XXX X XX X X X X X X X ANISOPTERAU55) Petaluridae (1) Tachopteryx thoreyi (Hagen in Selys) XX XX X X Aeshnidae (16) Aeshna constricta Say X X A. dugesi Calven X X X XX X A. multicolor Hagen XXX X X X X X X A. psilus Calvert X X X X A. umbrosa umbrosa Walker X XX X XXX Anax amazili (Burmeister) X X X A. junius (Drury) X X X X X XX X X X X X X X X A. longipes (Hagen) XX XX XX X A. walsinghatni McLachlan X X X X X X X Basiaeschna Janata (Say) XX XX XXX X Boyeria vinosa (Say) XX XX XX X Coryphaeschna ingens (Rambur) XX X X XX Epiaeschna hems (Fabricius) XX XX XX X XX Gomphaeschnafurcillata (Say) XX X X Gynacantha nen'osa Rambur X X X X Nasiaeschna pentacantha (Rambur) XX XX XX X XX Gomphidae (38) Aphylla angustifolia Garrison X X X X XX XX A. protracta (Hagen) X X X XX XX A. williamsoni (Gloyd) X * X Arigomphus lentulus (Needham) X XX X X A. mamelli (Ferguson) XX X X A. submedianus (Hagen) XX XX XX X A. villosipes (Selys) X X Dromogomphus armatus Selys X X D. spinosus Selys XX XX XX X D. spoliatus (Hagen in Selys) XX XX X X XX X XX Erpeiogomphus compositus Hag. in Selys X X X X X X X X £. crotalinus (Hagen in Selys) X X XXX £. designates Hagen in Selys X X X X X XXX X X X X X X X £. eutainia Calvert X X X X £. heterodon Garrison X X X X X £. lampropeltis Sampropeltis Kennedy X X X X X Gomphus (Gomphurus) externus Hagen X XXX X X X X X X X Vol. 109, No. 3, May & June, 1998 209 Table 1 (Continued) United States Mexico Biotic Provinces A R L A N M o K T X C C N T H O L A I A N M A U S T B A L C C H I H K N T A A A N V M S A A T E X A G. (G.) gomalezi Dunkle X X G. (G.) hybridus Williamson X X X X G. (G.) modestus Needham X X X X X G. (G.) ozarkensis Westfall X X X G. (G.) vastus Walsh X X X X X X X Gomphus (Gomphus) apomyius Donnelly X X X X G. exilis Selys X X X X G. graslinellus (Walsh) X X X X X X G. lividus (Selys) X X X X X G. militaris Hagen X X X X X X X XXX X G. oklahomensis (Pritchard) X X X X X X Hagenius brevistylus Selys X X X X X X X Ophiogomphus westfalli Cook & Daigle X X Phyilogomphoides albrighti (Needham) X X X X X X X X X X P. stigmatus (Say) X X X X X X X X X Progomphus borealis McLachlan X X X X X X X X P. obscurus (Rambur) X X X X X X X XXX X Stylogomphus albistylus (Hagen in Selys) X X X X Stylurus intricatus (Hagen) X X X X X 5. laurae (Williamson) X X X X S. plagiatus (Selys) X X X X X X X X X Cordulegastridae (3) Cordulegaster erronea Hagen in Selys X X X C. maculata Selys X X X X C. obliqua obliqua (Say) X X X X X X Corduliidae (24) Macromiinae (6) Didymops transversa (Say) X X X X X X X X Macromia alleghaniensis Williamson X X M. annulata Hagen X X X X X X X X X X M. illinoiensis georgina (Selys) X X X X X X X X X M. pacifica Hagen X X X X X X X M. taeniolaia Rambur X X X X X X Corduliinae(lS) Epitheca (Epicordulia) princeps Hagen X X X X X X X X X E. (Tetragoneuria) costalis (Selys) X X X X X X X E. (T.) cynosura (Say) X X X X X X X E. (T.) petechialis (Muttkowski) X X X X X X X X E. (T.) semiaquea (Burmeister) X X X X E. (T.) spinosa Hagen X X X X Helocordulia selysii (Hagen) X X X X H. uhleri Selys X X X Neurocordulia alabamensis Hodges X X X N. molesta (WaJsh) X X X X X X N. virginiensis Davis X X X X N. xanihosoma (Williamson) X X X X X X Somatochlora filosa (Hagen) X X * X 5. georgiana Walker X X X 5. linearis (Hagen) X X X X X X 5. margarita Donnelly X X X 5. ozarkensis Bird X X X S. lenebrosa (Say) X X X Libellulidae (73) Brachymesia furcata (Hagen) X XXX X X X X X B. gravida (Calvert) X X X X X X X X X B. herbida (Gundlach) X X X X X Brechmorhoga mendax (Hagen) X X X X X X X X X XXX X Cannaphila insularis/unerea (Carpenter) X X X X Celithemis amanda (Hagen) X X X X C. elisa (Hagen) X X X X X X X C. eponina (Drury) X X X X X X X X XXX X C.fasciata Kirby X X X X X X X C. ornata (Rambur) X X X C. verna Pritchard X X X X X 210 ENTOMOLOGICAL NEWS Table 1 (Continued) United States Mexico Biotic Provinces A L N 0 T R A M K X C C N T H O L A I A N M A B C K N T T U A H A A A E S L I N V M X T C H S A A A Dythemis fugax Hagen X XXX X X X X X X X X D. maya Calvert X X XX X X D. nigrescens Calvert X X X XX XX D. velox Hagen X X X X X X XX X X X X XX Erythemis collocata (Hagen) X X X X XXX E. plebeja (Burmeister) X X X XX E. simplicicollis (Say) X X X X X X X X X X X X X X E. vesiculosa (Fabricius) X X X XX X X X X Erythrodiplax berenice berenice (Drury) XX X X X X X X X E. connata (Burmeister) X X E.funerea (Hagen) X X X X E.fusca (Rambur) X X X X E. minuscula (Rambur) XX XX XX X E. umbrata (Linnaeus) XX XX X X X X X X XX Libellula auripennis Burmeister XX XX X X XX L. axilena West wood X X X L. comanche Calvert XXX X X X X X X L. composite (Hagen) X X XXX X L. croceipennis Selys XXX X X X X X X XX L. cyanea Fabricius X XX XXX X L. deplanata Rambur XX XX X X L. flavida Rambur XX XX XX X L. forensis Hagen X XXX L. incesta Hagen XX XX XXX X L. luctuosa Burmeister X X X X X X X X X X X X X L. lydia Drury X X X X X X X X X X X X X L. needhami Westfall XX X X X XX L. pulchella Drury X X X X X X X X X X X X L. saturata Uhler XXX XXX X X X X X X X L. semifasciata Burmeister XX XX X X L. subornata (Hagen) XXX X XXX L. vibrans Fabricius XX XX X X Macrodiplax balieata (Hagen) XX X X X X X XX Macrothemis imitans leucoiona Ris X X X X XX M. inacuta Calvert X X X X X M. inequiunguis Calvert X X X Miathyria marcella (Selys) XX X X X XX XX Micrathyria aequalis (Hagen) X X X X M. didyma (Selys) X X X M. hagenii Kirby X X X X XXX XX Orthemis ferruginea (Fabricius) X X X X X X X X X X X X X X X X Pachydiplax longipennis ( Burm.) X X X X X X X X X X X X X X Paltothemis lineatipes Karsch X X X X X X X X X X Pantalaflavescens (Fabricius) X X X X X X X X X X X X X X X X P. hymenaea (Say) X X X X X XXX X X X X X X X Perithemis domitia (Drury) X X X X P. lenera (Say) X X X X X XXX X X X X X X X Pseudoleon superbus (Hagen) X X X X XX XX Sympetrum ambiguum (Rambur) XX XX XXX S. corrupt urn (Hagen) X X X X X XX X X X X X X X X 5. costiferum (Hagen) X X 5. illotum (Hagen) X X X X 5. internum Montgomery X X X X S. occidentale fasciatum Walker X X XXX S. vicinum (Hagen) X XXX XX XX X Tauriphila azteca Calvert X X Tholymis citrina Hagen X X Tramea calverti Muttkowski X X XX T. Carolina (Linnaeus) XX XX XX X T. insularis Hagen X X T. lacerata Hagen X X X X X X X X X X X X X T. onusta Hagen X X X X X X X X X X X X X X Totals 127 112 82 132 1% 37 24 66 62 157 109 92 95 76 106 139 Vol. 1 09, No. 3, May & June, 1 998 211 LITERATURE CITED Abbott, J.C. 1996. New and interesting records from Texas and Oklahoma. Argia 8: 1 4- 1 5. Baumann, R.W. and B.C. Kondratieff. 1996. Ephemeroptera. In: Biodiversidad, taxonomia y biogeografia de artropodos de Mexico: Hacia una sintesis de su conocimiento, B.J. Llorente, A.A.N. Garcia and S.E. Gonzalez, eds. Univ. Nac. Autonoma de Mexico, Mexico 660 pp. Bick, G.H. 1983. Odonata at risk in conterminous United States and Canada. Odonatologica. 12:209-226. Bick, G.H. 1 957. The Odonata of Louisiana. Tulane Stud. Zool. 5:71-135. Bick, G.H. and J.G. Bick. 1957. The Odonata of Oklahoma. Southwest. Natur. 2:1-18. Blair, W.F. 1950. The biotic provinces of Texas. Texas J. Sci. 2:93-1 17. Blair, W.F. and T.H. Hubbell. 1 938. The biotic districts of Oklahoma. Amer. Mid. Natur. 20:425- 454. Calvert, P.P. 1901-1908. Odonata. In: Biologia Centrali-Americana, vol. 50: Neuroptera. 420 pp. Dice, L.R. 1943. The biotic provinces of North America. Univ. Mich. Press. Ann Arbor, MI. 78pp. Donnelly, T.W. 1 978. Odonata of the Sam Houston national forest and vicinity, east Texas, United States, 1960-1966. Notul. Odonatol. 1:1-16. Evans, M.A. 1995. Checklist of the Odonata of New Mexico with additions to the Colorado checklist. Proc. Denver Mus. Natur. Hist. 3: 1-6. Ferguson, A. 1940. A preliminary list of the Odonata of Dallas County, Texas. Field and Lab. 8:1-10. Ferguson, A. 1942. Scattered records of Texas and Louisiana Odonata with additional notes on the Odonata of Dallas County. Field and Lab. 1 0: 1 45- 1 49. Franklin, J.F. 1988. Structural and functional diversity in temperate forests, p. 166-175. In: Biodiversity, eds. E.G. Wilson and F.M. Peter. Natl. Acad. Press, DC. 521pp. Garrison, R.W. 1 99 1 . A synonymic list of the new world Odonata. Argia. 3: 1 -30. Garrison, R.W. 1995. The taxonomic status of twenty-five taxa of Odonata of the continental United States. Submitted to Nat. Biol. Surv. under Contract 84069-4-2738. 38 pp. Gloyd, L.K. 1958. The dragonfly fauna of the Big Bend region of trans-pecos Texas. Occ. Pap. Mus. Zool. Univ. Mich. 593:1-29. Gonzalez, S.E. and G.R. Novelo. 1991. Odonata de lareservade labiosfera laMichilia, Durango, Mexico. Parte I. Images. Folia Entomol. Mex. 81:67-105. Gonzalez, S.E. and G.R. Novelo. 1996. Odonata. In: Biodiversidad, taxonomia y biogeografia de artropodos de Mexico: Hacia una sintesis de su conocimiento, B.J. Llorente, A.A.N. Garcia and S.E. Gonzalez, eds. Univ. Nac. Autonoma de Mexico, Mexico 660 pp. HafTernik, J.E., Jr. 1989. Surveys of potentially threateneed bay area water beetles and the San Francisco forktail damselfly. Tech. Rep. U.S. Fish Wildl. Ag. Haffernik, J.E., Jr. 1992. Threats to invertebrate biodiversity: Implications for conservation strategies, pp. 171-195. In: Conservation biology: the theory and practice of nature conserva- tion preservation and management. P.L. Fiedler and S.K. Jain, eds. Chapman and Hall. N.Y. 507pp. Hagen, H. 1 86 1 . Synopsis of the Neuroptera of North America, with a list of South American species. Smithson. Misc. Coll. 4:1-347. Harp, G.L. and J.D. Rickett. 1977. The dragonflies (Anisoptera) of Arkansas. Ark. Acad. Sci. Proc. 31:50-54. Harp, G.L. 1983a. A preliminary report on the zygoptera (damselflies) of Arkansas. Arkansas Acad. Sci. Proc. 37:87-89. Harp, G.L. 1983b. New and unusual records of Arkansas Anisoptera, United States. Notul. Odonatol. 2:17-32. Harp, G.L. 1985. Further distributional records for Arkansas Anisoptera. Ark. Acad. Sci. Proc. 39:131-135. 2 1 2 ENTOMOLOGICAL NEWS Harp, G.L. and P.A. Harp. 1996. Previously unpublished Odonata records for Arkansas, Ken- tucky and Texas. Notul. Odonatol. 4: 127-130. Harwell, J.E. 1 95 1 . Notes on the Odonata of northeastern Texas. Texas J. Sci. 3:204-207. Johnson, C. 1972. The damselflies (Zygoptera) of Texas. Bull. Fla. State Mus., Biol. Sci. 16:55- 128. Kennedy, C.H. 1 92 1 . Some interesting dragon-fly naiads from Texas. Proc. U.S. Nat. Mus. 59:595- 598. Laswell, J.L. and F.L. Mitchell. 1997. Survey of dragonflies (Odonata: Anisoptera) in ponds of central Texas. J. Kans. Entomol. Soc. 70:52-63. McCafferty, W.P. and C.R. Lugo-Ortiz. 1996. Ephemeroptera. In: Biodiversidad, taxonomia y biogeografia de artropodos de Mexico: Hacia una sintesis de su conocimiento, B.J. Llorente, A.A.N. Garcia and S.E. Gonzalez, eds. Univ. Nac. Autonoma de Mexico, Mexico 660 pp. McCafferty, W.P., B.P. Stark, and A.V. Provonsha. 1990. Ephemeroptera, Plecoptera and Odonata, p.43-58. In: Systematics of the North American insects and arachnids: status and needs. M. Kosztarab and C.W. Schaefer, eds. Va. Polytech. Inst. State Univ., Blacksburg, VA. 247 pp. Moore, N.W. 1991 . Recent developments in the conservation of Odonata in Great Britain. Adv. Odonatol. 5:103-108. M ultkowski, R.A. 1910. Catalogue of the Odonata of North America. Bull. Pub. Mus. City Milwaukee. 1: 1-207. Novelo, G.R. and E.S. Gonzalez. 1 99 1 . Odonata de la reserva de la biosfera la Michilia, Durango, Mexico. Parte II. Nayades. Folia Entomol. Mex. 81:107-164. Paulson, D.R. 1982. Odonata. pp. 249-277. In: Aquatic biota of Mexico, Central America and the West Indies, S.H. Hurlbert and A. Villalobos-Figueroa, eds. S. Diego St. Univ., S. Diego, CA. Price, A.H., R.L. Orr, R. Honig, M. Vidrine, and S.L. Orzell. 1989. Status survey for the Big Thicket Emerald Dragonfly (Somatochlora margarita). Draft Report. Texas Parks Wild. Dept. Coop. Agreement No. 14-16-0002-86-925, Amndt. No. 7. Pronvonsha, A.V. and W.P. McCafferty. 1 973. Previously unkown nymphs of western Odonata (Zygoptera: Calopterygidae, Coenagrionidae). Proc. Entomol. Soc. Wash. 75:449- 454. Quintero, D. and A. Aiello, eds. 1992. Insects of Panama and Mesoamerica.. Oxford Univ. Press, Oxford, England. 720 pp. Szczytko, S.W. and K.W. Stewart. 1979. The genus Isoperla (Plecoptera) of western North America: holomorphology, systematics and a new stonefly genus Cascadoperla. Mem. Amer. Entomol. Soc. 32:1-120. Tinkham, E.R. 1934. The dragonfly fauna of Presidio and Jeff Davis Counties of the Big Bend Region of trans-pecos, Texas. Can. Entomol. 66:213-218. Thicker, E.S. 1908. Incidental captures of neuropterous insects at Piano, Texas. Psyche. 15:97- 100. USFWS, Department of Interior. 1996. Endangered and threatened wildlife and plants; review of plant and animal taxa that are candidates for listing as endangered or threatened. Federal Register 6 1:7596-76 13. Williams, C.E. 1 982. The dragonflies of McClennan County, central Texas, United States. Notul. Odonatologica 1:157-168. Williamson, E.B. 1914. Dragonflies collected in Texas and Oklahoma. Entomol. News. 25:41 1- 415,444-455. Young, W.C. and C.W. Bayer. 1979. The dragonfly nymphs (Odonata: Anisoptera) of the Guadelupe River basin, Texas. Texas J. Sci. 31:86-97. Vol. 109, No. 3, May & June, 1998 213 SCIENTIFIC NOTE: NEW DISTRIBUTIONS FOR RAPTOHEPTAGENIA CRUENTATA AND AMETROPUS NEAVEI (EPHEMEROPTERA: HEPTAGENIIDAE, AMETROPODIDAE)! R.D. Waltz,2 G. F. Edmunds, Jr?, Gary Lester4 Large river habitats possess some of the least known mayfly species in North America (McCafferty et al. 1990). Difficulty in sampling such habitats has undoubtedly contributed to the report of widely disjunct distributions of large river species. Decline in the quality of large river habitat has also possibly contributed to localized extirpations and further increased the apparent disjunction of reported distributions (see Whiting and Lehmkuhl 1987, McCafferty et al. 1990). Herein, two large river species, which are rarely collected, are newly reported from Montana. One of these two species is also newly reported from Minnesota. Raptoheptagenia cruentata (Walsh) has been reported previously from nine states or prov- inces in North America based on available literature (see Whiting and Lehmkuhl 1987, Edmunds and Waltz 1995). Reports of larval collections cited in the preceding papers include Arkansas, Illinois, Indiana, Montana, Ohio, and Saskatchewan. McCafferty (1988) designated the neotype of R. cruentata based on a larva in Indiana, which is housed in the Purdue Entomological Re- search Collection (PERC), West Lafayette, IN. Adult collections have been reported from Illi- nois, Indiana, Nebraska, Tennessee, and Manitoba. Two R. cruentata larvae taken in the Powder River, by G. Romero, with the following col- lection data: MT: Custer Co., Powder R., 11 -XI- 1976(1 larva), and same locale, 11 -VIII -1976 (2 larvae) were the source of the previously unpublished Montana record reported by Edmunds and Waltz (1995). In addition a single, nearly mature larva of R. cruentata was recently taken in collections made at the Montana state line in the Little Missouri River at MT: Carter Co., Little Missouri River, Sec 1 2, T 6S, R 62E, 1 7- VII- 1 996. An additional new state distribution report for R. cruentata is herein given for Minnesota as MN: Sibley Co., Minnesota R. (drift net), 30- VI- 1974, C.M. Haynes (1 young larva). The more rarely reported species Ametropus neavei McDunnough, was also collected in the Powder River from MT: Custer Co., Powder River, 1 1 -XI- 1976, G. Romero ( 1 larva). A. neavei was previously known from the type locality in Alberta and from Saskatchewan, Canada (Allen and Edmunds 1976) and Michigan (Steven and Hilsenhoff 1979). The recording of distributions of rare or rarely collected species is important 1 ) in order to provide data to adequately assess species rarity (e.g., see McCafferty and Edmunds 1997), and 2) to provide local and regional regulatory personnel and ecologists with data potentially critical to large stream management decision making. The above habitats in Montana are large streams with firm sand substrates. We do not have data characterizing the Minnesota habitat. However, detailed data from the Little Missouri River site is available through monitoring activities reported to us by Warren Kellogg, Watershed Spe- cialist, USDA NRCS, Helena, MT. Kellogg's data characterizes the Little Missouri at this site as 1 Received October 2, 1997. Accepted October 23, 1997. 2 IDNR, Division of Entomology and Plant Pathology, 402 West Washington, Room W-290, Indianapolis, IN 46204. 3 University of Utah, Department of Biology, Salt Lake City, UT84112. 4 EcoAnalysts, Inc., P.O. Box 3103, Moscow, ID 83843-1906. ENT. NEWS 109(3) 213-214, May & June, 1998 2 1 4 ENTOMOLOGICAL NEWS an intermittent, warm water stream. Channel catfish and carp were present in the immediate area. Physical characters available include: pH 8.6; temp 27° C; nitrates < 0.01 mg/1; TKN - 0.2 mg/1; OP-0.014 mg/1; TP-0.03mg/l; TSS-26mg/l. The specimen of R. cruentata taken was collected by means of a kick screen. The riparian vegetation includes patches of large cottonwoods (Populus deltoides) and willows (Salix spp.) encroaching on the sand/silt bars. There was little or no shade at the collection site. We thank Warren Kellogg for providing this data characterizing the collection site. Vouchers originating from the personal collection of George F. Edmunds, Jr., and the larva from Little Missouri River are deposited at the Purdue University Entomological Research Col- lection (PERC), West Lafayette, Indiana. LITERATURE CITED Allen, R.K. and G.F. Edmunds, Jr. 1976. A revision of the genus Ametropus in North America (Ephemeroptera: Ametropodidae). J. Kansas Entomol. Soc. 49: 625-635. Edmunds, G.F., Jr. and R.D. Waltz. 1995. Chapter 1 1 . Ephemeroptera. pp. 126-163. In: R.W. Merritt and K. W. Cummins (Eds.), An Introduction to the aquatic insects of North America, 3rd Edition. Kendall/Hunt, Dubuque, IA. McCafferty, W.P. 1988. Neotype designation for Raptoheptagenia cruentata (Walsh) (Ephe- meroptera: Heptageniidae). Proc. Wash. Entomol. Soc. 90: 97. McCafferty, W.P. and G.F. Edmunds, Jr. 1997. Critical commentary on the genus Siphlonisca (Ephemeroptera: Siphlonuridae). Entomol. News 108: 141-147. McCafferty, W.P., B.P. Stark, and A.V. Provonsha. 1 990. Ephemeroptera, Plecoptera, and Odonata, p. 43-58. In: Systematics of the North American Insects and Arachnids: Status and Needs, ed. M. Kosztarab and C.W. Schaefer. Virginia Agricultural Experiment Station Infor- mation Series 90-1. Blacksburg: Virginia Polytechnic Institute and State University. 247pp. Steven, J.C. and W.L. Hilsenhoff. 1 979. Ametropus neavei (Ephemeroptera: Ametropodidae) in the Upper Peninsula of Michigan. Great Lakes Entomol. 12:226. Whiting, E.R. and D. M. Lehmkuhl. 1987. Raptoheptagenia cruentata, gen. nov. (Ephe- meroptera: Heptageniidae), new association of the larva previously thought to be Anepeorus with the adult of Heptagenia cruentata Walsh. Can. Entomol. 119: 405-407. Vol. 109, No. 3, May & June, 1998 215 SCIENTIFIC NOTE: CLOEODES EXCOGITATUS (EPHEMEROPTERA: BAETIDAE) IN NORTHERN CALIFORNIA1 R.D. Waltz2 , Peter Ode3, Jon Lee4 The small minnow mayfly genus Cloeodes Traver is found in many different freshwater habitats throughout the Neotropics, Afrotropics, Asia and southern temperate areas of North America. Various revisionary works (Waltz and McCafferty 1987) and systematic works (Kluge 1991; Waltz 1993; Waltz and McCafferty 1994; McCafferty and Lugo-Ortiz 1995) have pro- vided taxonomic data for the genus and added greatly to our knowledge of the world distribution of the genus. The genus was recognized as having tropical affinities by Waltz and McCafferty (1987), McCafferty and Waltz (1990), and McCafferty et al. (1992). McCafferty and Lugo-Ortiz (1995) and Lugo-Ortiz and McCafferty (1993, 1994, 1995) further added to the known distribution of the genus in the Western Hemisphere, viz., southwestern North America, Mesoamerica, and South America. Herein, we report a remarkable, although possibly not unexpected, distribution record for this primarily subtropical and tropical genus from northern California. Larvae of C. excogitatus Waltz and McCafferty were taken in the following site in northern California: CA: Mendocino Co., Williams Crk near Covelo, 29-IX-1996. The above site is characterized by fine silt bottoms mixed with cobble. Stream temperatures are warm in the summer months. The above specimens were taken in low flow conditions near the end of the summer dry season. Lugo-Ortiz and McCafferty (1994, 1995) extended the known range of C. excogitatus from its type locality in Oak Creek Canyon, Arizona, southward to Yavapai County (Arizona) and Mexico. The type locale of C. excogitatus was previously the most northern report of the genus in North America. This report extends the known range of this species northward into North America by at least 600 miles reaching the Coastal Biotic Province of California (Usinger 1953) at approximately 40 degrees north latitude. Other northward extensions of primarily tropical genera into North America are also known in other mayfly genera, e.g., the baetid genera Camelobaetidius Demoulin and Paracloeodes Day (see McCafferty et al. 1992). Voucher specimens are deposited at the Purdue Entomological Research Collection, Purdue University, West Lafayette, IN and the California Academy of Science, San Francisco. LITERATURE CITED Kluge, N. 1991 . Cuban mayflies of the family Baetidae (Ephemeroptera). 1 . Genera Callibaetis, Cloeodes, and Paracloeodes. Zool. Zh. 12: 128-136. [in Russian]. Lugo-Ortiz, C.R and W.P. McCafferty. 1993. Genera of Baetidae (Ephemeroptera) from Cen- tral America. Entomol. News 104: 191-195. 1 Received October 31, 1997. Accepted December 17, 1997. 2 1DNR, Division of Entomology and Plant Pathology, 402 West Washington, Room W-290, Indianapolis, IN 46204. 3 Aquatic Biological Assessment Laboratory, California Department of Fish and Game, 2005 Nimbus Road, Rancho Cordova, CA 95670. 4 2250 Wilson Street, Arcata, CA 9552 1 . ENT NEWS 109(3) 215-216, May & June, 1998 216 ENTOMOLOGICAL NEWS Lugo-Ortiz, C. R. and W.P. McCafferty. 1994. New records of Ephemeroptera from Mexico. Entomol. News 105: 17-26. Lugo-Ortiz, C.R. and W.P. McCafferty. 1995. Annotated inventory of the mayflies (Ephemeroptera) of Arizona. Entomol. News 106: 131-140. McCafferty, W.P. and C.R. Lugo-Ortiz. 1995. Cloeodes hydation, n.sp. (Ephemeroptera: Baetidae): An extraordinary, drought tolerant mayfly from Brazil. Entomol News 106: 29- 35. McCafferty, W.P. , R.W. Flowers, and R.D. Waltz. 1992. The biogeography of Mesoamerican mayflies, pp. 173-193. In: S.P. Darwin and A.L. Weldon (eds.). Biogeography of Mesoamerica: proceedings of a symposium. Tulane Univ. Stud. Zool. Bot., Suppl. Publ. 1. McCafferty, W.P. and R. D. Waltz. 1990. Revisionary synopsis of the Baetidae (Ephemeroptera) of North and Middle America. Trans. Am. Entomol. Soc. 1 16: 769-799. Usinger, R.L. 1953. Introduction to Aquatic Entomology. Stream and Lake Classifications, pp 7-8 in Aquatic Insects of California, with keys to North American genera and California species. Univ. California Press, Berkeley. 508 pp. Waltz, R.D. 1993. Cloeodes binocularis (Ephemeroptera: Baetidae) a new combination for a Neotropical species of Pseudodoeon s. auctt. Entomol. News 104: 233-234. Waltz, R.D. and W.P. McCafferty. 1 987. Revision of the genus Cloeodes Traver (Ephemeroptera: Baetidae). Ann. Entomol. Soc. Am. 80: 191-207. Waltz, R.D. and W.P. McCafferty. 1 994. Cloeodes (Ephemeroptera: Baetidae) in Africa. Aquat. Insects 16: 165-169. Vol. 109, No. 3, May & June, 1998 217 SCIENTIFIC NOTE FIRST RECORD OF ALEIODES DEPANOCHORA (HYMENOPTERA: BRACONIDAE) FROM BRAZIL A. M. Penteado-Dias2 ABSTRACT: Aleiodes depanochora is recorded for the first time from Manaus, Descalvado and Dourado, Brazil. Aleiodes depanochora van Achterberg belongs to the Aleiodes dispar group in which males have peculiar depressions on the second and third metasomal tergites that are absent in females (van Achterberg, 1985). The Aleiodes dispar group includes eight species: three from the Old World, A. excavatus (Telenga, 1941 ), A. takasuae van Achterberg, 1 985 and A. yasirae van Ach- terberg, 1995, and five from the Neotropical region: A. elliptidepressus Penteado-Dias & van Achterberg, 1995, from Brazil, A. longipendulatus van Achterberg, 1985, from Costa Rica, A. rugosicostalis van Achterberg, 1995, from Peru, A. brevipendalatus van Achterberg, 1995, from Ecuador and A. depanochora, van Achterberg, 1995, from Peru. The localities listed below are the first records for A. depanochora from Brazil. Diagnosis: Aleiodes depanochora can be recognized by the following characters: head (ex- cept stemmaticum) yellowish-brown dorsally; side of pronotum dark brown or infuscate, paler parts not strongly contrasting with darker parts; metasomal tergites 1-3 partly infuscate; pterostigma evenly dark brown; hind tarsus infuscate or dark brown; length of malar space of male 1.1-1.2 times basal width of mandible and 0.3-0.35 times height of eye; face transversely rugose, dorsal face of pronotum in lateral view distinctly protruding; hind coxa densely trans- versely striate dorsally; first metasomal tergite more coarsely rugose laterally than medially and somewhat widened posteriorly; fourth tergite of male partly retracted and smooth; depression of second metasomal tergite of male without median carina and slender ovoid; median carina of second tergite of male 0.2-0.4 times length of depression; ratio of forewing veins 1-CUI: 2-CUI = 1:8-12, vein 3-SR of fore wing 1 .0 1.3 times as long as vein 2-SR. Specimens examined: 4 males, BRAZIL, Amazonas, Manaus, B. Klein, November 8,25,29, 1 984 (Instituto Nacional de Pesquisas da Amazonia); 1 male, BRAZIL, Sao Paulo, Descalvado, Fazenda Escaramucas, M. M. Dias, 9.XI, 1985 (light trap); 1 male, BRAZIL, Sao Paulo, Dou- rado, Fazenda Morro Chato, L. A. Joaquim, 26. V. 1994 (Departamento de Ecologia e Biologia Evolutiva da Universidade Federal de Sao Carlos, SP). The specimens from Manaus were collected in the Amazonian rainforest and those from Descalvado and Dourado were collected in Atlantic forest areas (Fig. 1 ). The discovery of the same species in the primary rain forest (Peru and Manaus) and in the secondary Atlantic forest (Dourado and Descalvado) is specially interesting. The biology of the Aleiodes dispar group species is unknown; the sparse data available on them indicate that they are parasites of lepidopterous larvae living in low vegetation (van Achterberg, & Penteado-Dias, 1 995). Same as Aleiodes yasirae van Achterberg, 1 995 and A. elliptedepressus van Achterberg & Penteado-Dias, 1995, we found A. depanochora in lowland vegetation, in a gallery forest close to a river. Probably that is the distribution pattern for Brazilian Aleiodes dispar group species and there we can find their hosts. 1 Received April 19, 1 996. Accepted September 8, 1997. 2 Departamento de Ecologia e Biologia Evolutiva, Universidade Federal de Sao Carlos. CP 676. CEP 13 565-905, Sao Carlos, SP, Brasil. ENT. NEWS 109(3) 217- 218, May & June, 1998 218 ENTOMOLOGICAL NEWS 80 70 60 10 10 20 30 Figure 1. Collection records of Aleiodes depanochora van Achterberg. ACKNOWLEDGMENTS I wish to thank the Institute Nacional de Pesquisas da Amazonia for the loan of the listed specimens. This work was supported by Conselho Nacional de Desenvolvimento Cientifico e Tecnologico and Funda9ao de Amparo a Pesquisa do Estado de Sao Paulo from Brazil. I wish to thank also C. van Achterberg, R. A. Wharton and P. Marsh who provided sugges- tions for improvements to the manuscripts. LITERATURE CITED Achterberg, C. van. 1985. The Aleiodes dispar group of the Palaearctic region (Hymenoptera: Braconidae: Rogadinae) Zool. Med. Leiden 59: 178-187, figs. 1-20. Achterberg, C. van & A.M. Penteado-Dias. 1 995. Six new species of the Aleiodes dispar group (Hymenoptera: Braconidae: Rogadinae). Zool. Med. Leiden (1):1-18, fig. 1-48. Vol. 109, No. 3, May & June, 1998 219 MEMBERS OF THE AMERICAN ENTOMOLOGICAL SOCIETY December 18, 1997 The previous list of 3 1 3 members was published in the September-October 1984 issue of Entomological News. That issue celebrated the 125th Anniver- sary of the Society. This present list contains 412 members from every state in the union (and the District of Columbia), except Alaska, Montana, Oregon and Rhode Island. The largest representation is from Pennsylvania (43). Interna- tional members represent 16 countries on the five continents. The format used in this list differs from the previous list in that all types of members appear in a single alphabetical list. Names of both Honorary and Life members are in bold face type. Names of Life members are further identified with an asterisk (*). The date shown is the year of record in our office when each member first joined the Society. I thank Suzanne McElroy for her assistance in the preparation of this list. Any corrections to this list can be sent to the Corresponding Secretary at the A.E.S. address on the inside front cover of this issue. Corrections will be pub- lished in a future issue of Entomological News. William J. Cromartie, Corresponding Secretary State or State or Surname Name Yr. Country Surname Name Yr. Country Abbott John C. 1991 TX Bilby Peter J. 1993 NJ Ahlstrom Kenneth R. 1996 NC Bilyj Bohdan 1995 Canada Ahn Kee-Jeong 1996 KS Bohart Richard M. 1967 CA Aime Cathie 1996 VA Bolton Michael J. 1987 OH Alexander Kevin D. 1993 NE Boobar Lewis Allen Robert T. 1988 DE Reginald 1996 ME Allred Miriam L. 1987 MS Bouseman John K. 1984 IL Amrine, Jr. James W. 1983 WV Bowles David E. 1985 TX Anderson Robert S. 1968 MD Boyd Howard P. 1939 NJ Armitage Brian J. 1980 OH Boys Frank E. 1970 DE Baker Charles W. 1980 ID Brigham, Jr. John A. 1986 CA Ball George E. 1963 Canada Brown John W. 1989 CA Balogh George J. 1987 MI Brown Kirby W. 1973 CA Barry Donald W. 1986 ME Brown Harley P. 1970 OK Bartlert Charles R. 1992 DE Brown Wendy S. 1993 CO Bartow Dennis H. 1970 PA Brown MarkW. 1997 WV Baumann Richard W. 1970 UT Brown, Jr. Keith S. 1970 Brazil Baumgardner David E. 1992 MD Brzoska David W. 1987 KS Beal, Jr. Richard S. 1970 AZ Burbutis Paul P. 1970 FL Beaman Carol A. 1996 TX Burdick Donald J. 1993 CA Beckemeyer RoyJ. 1979 KS Burger John F. 1980 Ntt Belish Timberley A. 1997 WY Burian Steven K. 1997 CT Bell Ross T. 1985 VT Burrows Weldon L. 1983 WV Berk Mary 1989 NJ Bushey Sonja Marie 1994 NY ENT. NEWS 109(3) 219-223, May & June, 1998 220 ENTOMOLOGICAL NEWS State or State or Surname Name Yr. Country Surname Name Yr. Country Butler Linda 1979 WV Dunkle Sidney W. 1983 TX Bystrak Paul G. 1991 MD Durfee Richard 1994 CO Cancellare Joseph A. 1977 TX Earle Jane 1993 PA Canterbury Lawrence E. 1975 OH Edmunds George F. 1948 UT Canton Steven P. 1978 CO Edwards Robert L. 1990 MA Carlton Chris E. 1996 LA Elliott Douglas E. 1984 CA Caron Dewey M. 1983 DE Elmali Meryem 1996 Turkey Carter Janet L. Stein 1986 OH Enns Wilbur R. 1971 MO Cauble Ronald L. 1993 CA Epler John H. 1976 FL Chandler Donald S. 1985 NH Erikson JeffS. 1994 PA Chandler, Jr. Jack H. 1996 GA Estes William J. 1985 PA Chiba Hideyuki 1988 Japan Ettinger William H. 1986 IL Chordas III Steve W. 1997 OH Ettinger William S. 1979 PA Ciborowski Jan J. H. 1978 Canada Evans Howard E. 1973 CO Ciegler Janet C. 1996 SC Fall Louise H. 1985 CA Ciurlino Randolph A. 1994 DE Fava Jane F. 1997 PA Clausen Philip J. 1983 MN Fee Frank D. 1979 PA Coher Edward I. 1994 NY Ferreira Raul N. 1994 CT Conklin, Jr. DonJ. 1988 CO Fisher Stephen C. 1994 PA Conn D. Bruce 1991 GA Fisher Elizabeth 1945 MD Contreras- Flemyng E. Penryn 1986 CA Ramos Atilano 1986 Mexico Flint, Jr. Oliver S. 1982 DC Cook Jerry L. 1995 TX Floyd Michael A. 1991 KY Cooper Kenneth W. 1946 CA Forbes Gregory S. 1977 NM Coovert Gary A. 1997 OH Foye Laurene 1994 SD Courtney Gregory W. 1991 IA Franchine Michael J. 1997 PA Covell, Jr. Charles V. 1969 KY Frank Kenneth D. 1979 PA Cromartie William J. 1991 NJ Frank Susan E. 1979 PA Currie Charles E. 1992 CA Fredrickson Richard W. 1983 PA Cuthrell David L. 1996 Ml Froeschner Richard C. 1970 DC Cutler Bruce 1991 KS Fuester Roger W. 1965 DE Dakin, Jr. Matt E. 1971 AL Fullerton Stuart M. 1990 FL Darlington Mark B. 1994 NJ Funk David H. 1987 PA Davis Jack R. 1991 TX Gage Ed 1986 TX Davis Link M. 1995 PA Galford Jim 1980 OH Day William H. 1966 DE Ganeo de Francisco de De Souza Paulo Mello Assis 1991 Brazil Moutinho Roberto 1995 Brazil Gayubo Severiano F. 1993 Spain DeWalt R. Edward 1991 IL Gelhaus JonK. 1987 PA Deyrup Mark A. 1978 FL Giesecke Martiin 1979 TX Dolan Michael F. 1997 MA Gomez-Arias Luis M. 1992 FL Donelan Laurence W. 1995 FL Goodrich Michael A. 1989 IL Dougherty Veronica M. 1984 MD Gordon Robert D. 1969 ND Dozier Herbert L. 1958 SC Gottschalk Steven C. 1996 IA Drecktrah H. Gene 1971 WI Graham Alan C. 1993 VT DuBois MarkB. 1988 IL Grant Peter M. 1984 OK Duffield Richard M. 1995 DC Graves Robert C. 1967 OH Duncan Paul M. 1963 PA Greathouse Zane B. 1997 FL Vol. 109, No. 3, May & June, 1998 221 State or State or Surname Name Yr. Country Surname Name Yr. Country Griffith Michael B. 1992 PA Kingsolver John M. 1969 FL Grubbs Scott A. 1991 PA Kirchner Ralph F. 1976 WV Gusten Robert 1995 Germany Kirchenstein Barbara B. 1989 PA Haack Robert A. 1996 Ml Kistner David H. 1985 CA Halstead Jeffrey A. 1985 CA Klubertanz TomH. 1997 NE Hamilton Robert W. 1989 IL Knight JeffB. 1963 NV Hamilton Steven W. 1987 TN Knisley C.Barry 1983 VA Hanley Rodney S. 1992 KS Knizeski, Jr. Henry M. 1985 NY Hansen Dean 1990 MN Koehn Leroy C. 1996 MS Harrington DonG. 1989 TX Kondratieff Boris C. 1982 CO Harris Steven C. 1982 PA Koski Joseph T. 1962 MA Hastriter Michael W. 1996 UT Krinsky William L. 1987 CT Heck Mary L. 1995 OH Kritsky Gene 1978 OH Henry, Jr. Brad C. 1984 TX Krombein Karl V. 1972 DC Heppner John B. 1997 FL Krotzer Steve 1991 AL Herbst David B. 1996 CA Krysan James L. 1981 KY Herrmann Scott J. 1985 CO LaBerge Wallace E. 1961 IL Heth Robert K. 1996 OK Lacey MarkS. 1994 DE Hildebrandt Drew A 1984 MS Lago Paul K. 1981 MS Hill Richard E. 1993 CA Lanteri AnaliaA. 1991 Argentina Hill Russell E. 1987 FL Lantsov Vladimir I. 1996 Russia * Hilton Donald F. J. 1986 Canada Larsen Eric 1995 PA Hodges R.W. 1960 DC Larson Omer R. 1982 ND Hoebeke E. Richard 1988 NY Lasalle MarkW. 1979 MS Hoffman Kevin M. 1987 CA Ledin Kathryn E. 1993 SC Holdeman Steven J. 1996 TN Lee Sharon D. 1995 PA Holzbach John E. 1975 OH Leschen Richard A. B 1986 Australia Homer Norman 1987 TX Lester Gary T. 1993 ID Houghton David C. 1997 TX Levesque Claire 1985 Canada Huckstep E. Elgin 1990 CA Levy Stephen D. 1992 PA Hudson Patrick 1985 MI Lewis Robert E. 1956 IA lanni Charles 1985 OH Lewis Carolyn N. 1997 AR Iftner David C. 1993 NJ Lillie Richard A. 1997 WI Insley Sandra Lingafelter Steven W. 1997 DC Yawetz 1995 OH Linsley E. Gorton 1962 CA Jackson John K. 1990 PA Lipinski Daniel R. 1989 NJ Jasper Sharon Livingston Margot 1987 NJ Knight 1995 TX Livingston CarlF. 1993 NJ Jennings Daniel T. 1969 ME Livingston Rosemary A. 1993 NJ Johnson Zane B. 1997 TX Lowe Graeme 1988 PA Katovich Kerry 1994 WI Mac Neill C.Don 1995 CA Kavanaugh David H. 1995 CA Mackay William 1996 TX Kearns Ruth S. 1994 DE Mackenzie Allen H. 1987 SC Kedanis Richard J. 1997 PA Majerowicz Eugene I. 1991 CA Keiper JoeB. 1996 OH Manley GaryV. 1980 MI Kelley Richard I. 1988 PA Marque/, Luna Juan 1994 Mexico * Kennedy James H. 1980 TX Marshall Brett D. 1997 PA Kidd Kathleen A. 1992 NC 222 ENTOMOLOGICAL NEWS State or State or Surname Name Yr. Country Surname Name Yr. Country Martinez Humberto Quiroz 1989 Mexico Penteado-Dias Angelica Marx Rainer Peter 1996 Germany Maria 1994 Brazil Mason Charles E. 1976 DE Perez Juan Mathis Wayne N. 1977 DC Dominguez Francisco 1988 Mexico Matsunaga Wallace 0. 1985 IL Peters William L 1998 FL Matta James F. 1974 PA Pfadt Robert E. 1988 WY May Michael L. 1992 NJ Philtower Ramona 1993 DE McCabe Tim L. 1997 NY Philips James R. 1982 MA McCafferty W. Patrick 1987 IN Pinter Lawrence J. 1980 HI McCaleb John E. 1996 AL Plan Austin P. 1988 MD McCauley Luana M. M. 1995 ID Plotnikoff Robert W. 1995 WA McHugh Joseph V. 1989 GA Plummer John A. 1990 DE Michener Charles D. 1963 KS Polhemus John T. 1996 CO Miller LeD. 1967 FL Porter Charles H. 1968 GA Miller William B. 1993 TX Preston Floyd W. 1980 KS Miller Jacqueline Y. 1989 FL Price Michele B. 1997 IL Molnar Steven A. 1997 NJ Purrington Foster F. 1997 OH Morrison Michael W. 1993 ME Ranger Christopher Morse John C. 1980 SC M. 1997 NJ Moulton 11 Stephen R. 1987 CO Rayburn Brian S. 1997 OH Muchmore William B. 1989 NY Rebollar-Tellez Eduardo A. 1993 United Muniz Velez M.enC. Raul 1992 Mexico Kingdom Munro James B. 1995 PA Rentz David C.F. 1965 Australia Murphy Clint 1991 MO Rhodes Howard A. 1996 CO Murvosh Chad M. 1991 NE Richards Austin Brady 1996 CO Myles Timothy G. 1986 Canada Rider David A. 1987 ND Naczi Robert F.C. 1983 KY Riley Edward G. 1986 TX Namkaidorj B. 1996 Mongolia Ringgier Theodore G. 1992 MD Navarrete- Risley Lance S. 1989 NJ Heredia Jose Luis 1992 Mexico Ritter Karla S. 1979 Wl Neff Stuart E. 1988 PA Robbins Richard G. 1988 MD Nelson Charles H. 1969 TN Roberts Richard H. 1971 FL Nelson S.Mark 1991 CO Robinson Jeffrey M. 1995 KY Nelson C. Riley 1984 TX Robison Henry W. 1990 AR Nelson Harry G. 1982 IL Romig Ronald F. 1979 PA Nielson M.W. 1992 UT Roth Louis M. 1957 MA Novinger James S. 1991 CO Rothschild MarkJ. 1987 MD O'Brien Charles W. 1993 FL Ruesink William G. 1988 IL O'Donnell Sean 1992 WA Ruffin JaneM. 1988 PA O'Keefe Sean T. 1991 CA Ruiter David 1976 CO Ocus Warren G. 1992 MD Russell DanaC. 1992 CA Omer John R. 1990 WV Rust Richard W. 1974 NV Otte Daniel 1976 PA Sabourin Michael 1993 WI Parrott Rod 1977 Canada Salmon J.T. 1960 New Payne Randall G. 1993 FL Zealand Pennington Wendell L 1985 TN Sandridge Paul T. 1991 DE Vol. 109, No. 3, May & June, 1998 223 State or State or Surname Name Yr. Country Surname Name Yr. Country Santiago de Tennessen Kenneth J. 1996 AL Bueno Silvia 1994 Mexico Thompson F.C. 1969 VA Sarver Randy J. 1996 MO Tinerella Paul P. 1996 ND Saunders Robert D. 1990 Canada Torres Felix 1993 Spain Scarbrough Aubrey G. 1987 MD Torres-Miller Laura 1997 WV Schaefer Paul W. 1985 MD Trond Andersen 1997 Norway Schesser, Jr. James F. 1978 KS Turnbow, Jr. Robert H. 1983 AL Schiefer Terence L. 1984 MS Turner Alan R. 1994 N.C. Schmidt Justin 0. 1987 AZ Valenti Michael A. 1985 DE Schmude KurtL. 1992 Wl Ventre Vincent 1985 PA Schroder Robert F. W. 1993 MD Vogtsberger RoyC. 1989 TX Schweitzer Dale 1989 NJ Wagner Michael R. 1980 AZ Seaborg Norman G. 1963 IL Wahl David B. 1984 FL Seltmann Katja Chantre 1996 GA Walls Jerry G. 1984 NJ Shapiro Arthur M. 1967 CA Waltz Robert D. 1983 IN Sheldon Joseph K. 1973 PA Webb David K. 1996 MI Shepard William D. 1979 CA Weber Neal A. 1961 FL Short Andrew E. 1996 DE Weber Richard G. 1986 DE Shubeck Paul P. 1971 NJ * Weisse Theodore H. 1987 NY Sibley Paul K. 1989 MN Weissmann Michael J. 1988 CO Sikes Derek S. 1996 CT Wesson Laurence G. 1995 PA Sissom William Wheatley John B. 1995 PA David 1995 TX Wheeler Jeanette N. 1970 FL Skelley Paul E. 1991 FL White Harold B. 1975 DE Slayback Scot E. 1997 MD White David S. 1976 KY Sleeper Elbert L. 1971 CA White Timothy M. 1996 SC Smith David R. 1966 D.C. Whitehead V.B. 1976 S. Africa Smith Stephen M. 1993 Canada Whitfield James B. 1993 AR Sneen Martin E. 1993 IL Whitney Susan P. 1990 DE Snider Richard J. 1985 MI Wiersema Nick 1997 TX Sobat Thomas 1996 IN Wiker James R. 1990 IL Spangler Hay ward G. 1990 AZ Willemse Per 1968 Netherlands Spokony Harvey 1997 NY Williams David W. 1988 PA Spooner John D. 1986 SC Williams Roger N. 1991 OH Springer Charles A. 1992 NE Williams J. Logan 1995 NC Staines Charles L. 1986 MD Willink Abraham 1963 Argentina Stallings Viola N. 1988 KS Wilson Stephen W. 1992 MO Stein Raymond J. 1988 NJ Wimmer H. Peter 1979 VT Stewart Kenneth W. 1994 TX Wojtowicz John A. 1983 TN Stidham John A. 1982 TX Wolinski Jeffrey A. 1988 MD Strassmann JoanE. 1983 TX Wright David M. 1995 PA Strazanac John S. 1984 wv Young Daniel K. 1980 WI Sublette James E. 1990 AZ Young OrreyP. 1980 MD Summerville Keith S. 1996 MI Young Wayne L. 1986 PA Surman Michael A. 1976 LA Zeigler David D. 1990 NC Tarter Donald C. 1972 WV Zoidis John 1996 Greece Taylor Steven J. 1988 IL Zuccaro, Jr. Anthony E. 1981 MS 224 ENTOMOLOGICAL NEWS SOCIETY MEETING OF NOVEMBER 19, 1997 Dr. Jim Marden Dept. of Biology Perm State University Dr. Marden began by pointing out the evolution of flight is a classic challenge for Darwinian natural selection, for it is difficult to envision how gradual, incremental selection can result in complex traits that function only in their fully developed form. The fossil record offers little help in determining the origin of insect wings and flight, for at the time winged insects first appear in fossils (325mya), they had already radiated and diversified into stem groups of all of the major lineages present today. Thus, we are left to decipher the evolutionary history of insect flight from our judgment of which traits of fossils and extant insects most closely represent the ancestral con- dition. The emergent view from this body of research is that insect wings arose from moveable, articulated gills of aquatic ancestors, and that the orders Ephemeroptera (mayflies) and Plecoptera (stoneflies) have the most primitive morphology among extant winged insects. Dr. Marden 's laboratory has recently proposed that surface-skimming, a form of nonflying aerodynamic locomotion used by certain modern stoneflies, is a feasible intermediate stage be- tween swimming and flying, and might be a remnant of the ancestral condition for all winged insects. This hypothesis has been lauded as the most reasonable model for a transitional process offered to date, but it has also been criticized for failing to consider the phylogenetic position of skimming stoneflies. In his presentation, he gave an overview of work examining i) functional aspects of various forms of surface skimming, ii) preliminary results of a molecular phylogeny of stoneflies using 18S rDNA sequence data, and iii) the phylogenetic distribution of skimming be- havior across the Plecoptera. These results suggest that surface skimming is a primitive trait among stoneflies, and thus a likely candidate for a transitional stage in the evolution of insect flight. The talk was illustrated with many striking slides and a video of surface-skimming flight. In insect-related news, Jon Gelhaus circulated a specimen of Dermatobia hominis larva (Diptera) recently excised from a member of the ANS staff forty-five days after a trip to Guyana. The adult of this parasite catches a mosquito, to which it attaches its offspring, and the mosquito passes it on to a human. Mention was also made of impending trials of two Lyme disease vaccines by Philadelphia area pharmaceutical firms. - W. J. Cromartie Corresponding Secretary ENT. NEWS 109(3) 224, May & June, 1998 When submitting papers, all authors are requested to (1) provide the names of two qualified individuals who have critically reviewed the manuscript before it is submitted and (2) suggest the names and addresses of two qualified authorities in the subject field to whom the manuscript may be referred by the editor for final review. All papers are submitted to recognized authorities for final review before acceptance. Titles should be carefully composed to reflect the true contents of the article, and be kept as brief as possible. Classification as to order and family should be included in the title, except where not pertinent. Following the title there should be a short informative abstract (not a descriptive abstract) of not over 150 words. The abstract is the key to how an article is cited in abstracting journals and should be carefully written. It should be an informative digest of the significant contents and of the main conclusions of the research. The author's com- plete mailing address, including zip code number, should be given as a footnote to the arti- cle. All papers describing new taxa should include enough information to make them useful to the nonspecialist. Generally this requires a key and a short review or discussion of the group, plus references to existing revisions or monographs. Authors must include the name(s) of recognized institution(s) where their voucher specimens have been deposited. Illustrations nearly always are needed. All measurements shall be given using the metric system or, if in the standard system, comparable equivalent metric values shall be included. 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The following books are available from Kendall/Hunt Publishing: An Introduction to the Aquatic Insects of North America edited by Richard W. Merritt and Kenneth W. Cummins (1995/880 pages/wire coil/$69.95*/lSBN 0-7872-3241-6 or 1995/880 pages/otabind/S78.69*/ ISBN 0-7872-3240-8). Aquatic Insects, with readings written by 41 experts, will quickly become your standard reference book. It includes features such as: com- prehensive coverage of behavior, collecting, biomonitoring, and taxonomy; well-illustrated keys to major life stages of North American aquatic insects; and tables at the end of every identification chapter with summaries at the generic level of the ecology, habits, and distribu- tion of the order or family of aquatic insects. Immature Insects, Volumes I and II, edited by Frederick W. Stehr (Volume I: 1 987/768 pages/ casebound/$136.44*/ISBN 0-84034639-5 and Volume II: 1991/992 pages/casebound/ 241.44*/ISBN 0-8403-4639-5). Immature Insects provides information on the biology and ecology of the families and selected important species. The two volume set also gives you a means to identify insects ranging from the most common to the extremely rare through use of the abundant illustrations, descriptions, and/or keys to selected species. Immature Insects is the only reference that extensively covers updated information necessary to identify imma- ture insects. These books also describe techniques necessary for the collecting, rearing, kill- ing, preserving, storing, and studying of insects. Both books also include an introduction defining how terms are used in the book, a complete glossary, and an extensive index. For more information or to place an order, call Jill Crow at 1-800-228-0564. *A11 prices are subject to change. FOR SALE: Quality insect pins, black enamelled, stainless steel. Best prices guaranteed. Call for free samples. Phone: 1 (800) 484-7347 Ext. 1324. Fax: (352) 37 1-69 18. E-mail: morpho@afn.org or write to Morpho Ventures, P.O.BOX 12454, Gainesville, Florida 32604. FOR SALE: Baltic amber pieces with insects, spiders, plants. Material for scientific work, as well as better pieces for display and teaching. O. Holden, Junkerg. 37, S-126 53 Hegersten, Sweden, fax: 01146-8-7268522. FOR SALE: Light traps, 12 volt DC or 1 10 volt AC with 15 watt or 20 watt black lights. Traps are portable and easy to use. Rain drains and beetle screens protect specimens from damage. For a free brochure and price list, contact Leroy C. Koehn, 207 Quail Trail, Greenwood, MS 38930-7315. Telephone 601-455-5498. VOL. 109 SEPTEMBER & OCTOBER, 1998 US ISSN 0013-872X NO. 4 I ENTOMOLOGICAL NEWS n Mexican species ofGastrisus (Coleoptera: taphylinidae) J.L. Navarrete-Heredia, J. Marquez 225 £_ Aj'T'"" aism in Neogerris hesione (Heteroptera: Jerridae) in southern Illinois S.J. Taylor, J.E. McPherson 233 A new species of Paruroctonus (Scorpiones: Vaejovi- dae) from Big Bend National Park, Texas W.D. Sissom, R. N. Henson 240 Comparison of sand nesting wasps (Hymenoptera) from Frank E. Kurczewski 247 two pine barrens areas in upstate New York The identity of Tachysphex acutus (Hymenoptera: Sphecidae), an unsolved mystery Character variability & a new synonym of Acerpenna pygmaea (Ephemeroptera: Baetidae) R.D. Waltz, D.E. Baumgardner, J.H. Kennedy Reared association & equivalency of Baetis adonis & B. caelestis (Ephemeroptera: Baetidae) W. P. McCafferty, E. L. Silldorff Additions & corrections to Ephemeroptera species of North America & index to their complete nomenclature W.P. McCafferty Taxonomic notes on Evaniodini (Hymenoptera: Braconidae), with redescription of Evaniodes spathiiformis & description of a new species SM. Barbalho, A.M. Penteado-Dias Zorcadium Bergroth, an objective junior synonym of Pseudobebaeus Fallou (Heteroptera: Pentatomidae) DA. Rider, C. Fischer Mass appearance of lady beetles (Coleoptera: Coccinellidae) on North Carolina beaches CA. Nalepa, K.R. Ahlstrom, BA. Nault, J.L. Williams Biological & morphological notes on Dasyhelea pseudo- incisurata (Diptera: Ceratopogonidae) Lawrence J. Hribar Eastward range extension in Canada of the alderfly Sialis velata (Megaloptera: Sialidae), & the potential of the genus as a contaminant monitor /. Roy, L. Hare Gynandromorph of Helicoverpa armigera (Lepidoptera: Noctuidae) A. Josephrajkumar, B. Subrahmanyam, V.V. Ramamurthy A new species of Sweltsa (Plecoptera: Chloro- perlidae) from eastern No. America B.C. Kondratieff, R.F. Kirchner SCIENTIFIC NOTES Incorporation of Batrachospermum gelatinosum (Rhodophyta) into cases of Ochrotrichia wojcickyi (Trichoptera: Hydroptilidae) J.B. Keiper, DA. Casamatta, BA. Foote Additions to Iowa mayflies (Ephemeroptera) W.P. McCafferty, T. Hubbard BOOK REVIEW BOOKS RECEIVED & BRIEFLY NOTED ANNOUNCEMENT SOCIETY MEETING of March 25, 1998 SOCIETY MEETING of April 22, 1998 Frank E. Kurczewski 252 257 261 269 274 277 282 285 288 293 NOV 0 9 1998 THE AMERICAN ENTOMOLOG ENTOMOLOGICAL NEWS is published bi-monthly except July-August by The American Entomological Society at the Academy of Natural Sciences, 1900 Benjamin Franklin Park\vay, Philadelphia. Pa.. 19103-1195. U.S.A. The American Entomological Society holds regular membership meetings on the fourth Wednesday in October, November, February, March, and April. The November, February and April meetings are held at the Academy of Natural Sciences in Philadelphia, Pa. The October and March meetings are held at the Department of Entomology, University of Delaware, Newark, Delaware. Society Members who reside outside the local eastern Pennsylvania, southern New Jersey, and Delaware area are urged to attend society meetings whenever they may be in the vicinity. 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Papers on applied, economic and regulatory entomology and on toxicology and related subjects will be considered only if they also make a major contribution in one of the aforementioned fields. (Continued on inside of back cover) Postmaster: // undeliverable, please send form 3579 to Howard P. Boyd, 232 Oak Shade Road, Tabernacle Twp., Vincentown, New Jersey 08088, U.SA. SECOND CLASS POSTAGE PAID AT VINCENTOWN, NEW JERSEY, 08088, U.S.A. Vol. 109, No. 4, September & October, 1998 225 A NEW MEXICAN SPECIES OF GASTRISUS (COLEOPTERA: STAPHYLINIDAE)1 Jose Luis Navarrete-Heredia^, Juan Marquez^ ABSTRACT: Gastrisus newtonorum, new species, is described based on specimens from the states of Mexico, Guerrero, Jalisco and Morelos. One specimen was examined by Bernhauer, however it was misidentified as G. mimetes Sharp. The Mexican record of G. mimetes in Bemhauer and Schubert's and Blackwelder's catalogs was probably based on this misidentification. The two species are compared, and the aedeagi are illustrated. Distributional and biological data are provided. Gastrisus Sharp, 1876 is an American genus,with most species in South America. At present, 19 species are recognized: Blackwelder (1944) cited 12 species; Scheerpeltz (1972) moved six species from Trigonopselaphus to this genus; and one more is described here. Gastrisus mimetes Sharp was described based on a single specimen from Costa Rica (Sharp, 1884: 360). Years later, Bernhauer and Schubert (1914) and Blackwelder (1944) recorded this species from Mexico. However, we do not know if the Mexican record was ever published elsewhere. Bernhauer (1912: 39) in his description of G. venezolanus mentioned that he had identified the holotype earlier as G. mimetes, and that he was comparing it to specimens of G. mimetes from Colombia and Peru (country records not included in the cata- logs cited above). One specimen from Guerrero (at FMNH), identified by Bernhauer as G. mimetes belongs to an undescribed species. This situation was first recognized by A.F. Newton, Jr. who compared one specimen from Guerrero with the holo- type of G. mimetes at BMNH (see material examined). Later, he received speci- mens from G. Ruiz-Lizarraga and L. E. Rivera collected in Guerrero and Jalisco respectively, that he identified as Gastrisus n. sp. Ruiz-Lizarraga (1993), in her excellent contribution on carrion staphylin- ids, provided descriptions of the species that she collected, and included one for Gastrisus n. sp. From that date until now this species has awaited formal description. Upon request, Dr. Newton kindly sent us specimens of the undescribed species from FMNH and four G. mimetes from Costa Rica to support this work. Also, Miss G. Ruiz-Lizarraga allowed us to describe this species. The goal of this paper is to describe and provide biological data on this new species. 1 Received September 12, 1997. Accepted November 19, 1997. Entomologia, Centro de Estudios en Zoologia, CUCBA, Universidad de Guadalajara, Apdo. Postal 234, 45100 Zapopan, Jalisco, Mexico. Lab. de Morfofisiologia Animal, Fac. de Ciencias, UNAM, 04510, Mexico, D.F. ENT. NEWS 109(4) 225-232, September & October, 1998 226 ENTOMOLOGICAL NEWS MATERIALS AND METHODS Specimens for this study were borrowed from: Field Museum of Natural History, Chicago (FMNH); Coleccion Entomologica, Escuela Nacional de Estudios Profesionales, Iztacala (ENEPI); and Coleccion Entomologica, Universidad de Costa Rica (UCR); others were collected by the authors during their research. Acronyms for collections where type material will be deposited are: American Museum of Natural History, New York ( AMNH); British Mu- seum, London (BMNH); Canadian National Collection, Ottawa (CNC); Entomologia, Centre de Estudios en Zoologia, Universidad de Guadalajara, Zapopan (CZUG); Institute de Biologia, UNAM, D.F. (IBUNAM); Snow En- tomological Museum, Lawrence, Kansas (SEM); Laboratorio Especializado de Morfofisiologia Animal, Fac. de Ciencias, UNAM, D.F. (LEMA); Juan Marquez Luna Collection, D.F. (JML); Jose Luis Navarrete Collection, Zapopan (JLN); and Museo de Historia Natural Ciudad de Mexico, D.F. (MHNCM). Throughout this paper we refer to abdominal segments by their morpho- logically comparable names and use roman numerals for these. The first fully visible segment is segment III. Tergum II is usually narrowly visible. Total length was measured from the anterior margin of the head to the apex of ab- dominal segment IX. Gastrisus newtonorum Navarrete and Marquez, NEW SPECIES Figs. 1,2,4,5,8. HOLOTYPE MALE: Length 1 5.6 mm. Black, except abdominal segments VII-IX, last seg- ment of maxillary and labial palpi, and tarsi rufotestaceous. Surface covered with microsculpture consisting of isodiametric meshes, mixed with scattered micropunctures; tempora with irregular waves; neck and abdominal segments with dual microsculpture: isodiametrical meshes at base and irregular waves on apical portion (more distinct on segments V1I-VIII). Head subquadrate; setiferous punctures denser at posterior angles and along medial borders of eyes, dorsal surface without setiferous punctures (Fig. 2). With subocular ridge. First antennal segment slightly shorter than next two segments combined, second segment shorter than third segment; fifth to eleventh transverse, large setae decreasing in number but short setae more conspicuous. Second and third segments of maxillary palpi broader at apex, last segment elon- gate, subcylindrical, as long as second segment. Last segment of labial palpi as broad as penultimate segment and as long as last segment of maxillary palpi. Mandibles subequal in length to head along midline. Right mandible with a tooth opposite an emargination of left mandible. Gular sutures confluent at middle. Neck with oblique longitudinal line dorsolaterally on each side. Pronotum slightly larger than head; narrowed toward base; anterior angles rectangular, basal angles obtuse; setiferous punctures scattered, denser at sides, dorsal punctures 3:3, asymmetri- cal; postcoxal process of the hypomeron translucent. Elytra opaque, with numerous setiferous punctures, with two humeral macrosetae and one near scutellum. Two macrosetae on anterior middle of prosternum. Tibiae with spines, denser on mesotibiae. First four segments of anterior tarsi dilated, as broad as anterior tibiae, with modified pale setae ventrally; last segment as long as previous three segments combined. Middle and hind tarsi similar: first segment as long as following three segments combined, last segment as long as previous two segments combined. Abdominal segments as shining as head and pronotum; tergites II1-IV with impressed line Vol. 109, No. 4, September & October, 1998 227 1 mm Figure I . Dorsal view of Gastrisus newlonorum Navarrete and Marquez, new species. 228 ENTOMOLOGICAL NEWS 1 mm Imm 7 0.1mm Figures 2-9. Morphology ofGastrisus spp. G. newtonorum: 2. Head and pronotum, 4. Aedeagus (parameres removed), 5. Paramere, 8. Distribution of peg setae on paramere. G. mimetes. 3. Head and pronotum, 6. Aedeagus (paramere removed), 7. Paramere, 9. Distribution of peg setae on paramere. Vol. 109, No. 4, September & October, 1998 229 on basal portion. One black macroseta on each side of posterior border of terga III-VI. Sternite VII slightly emarginate at middle; VIII with conspicuous triangular emargination; sternites VII- VIII with three black macrosetae on each side. Sternite IX emarginate, with two black macrosetae. Aedoeagus as in Figs. 4-5, 8. Paramere with apex almost reaching apex of medial lobe. ALLOTYPE FEMALE: Length 14.1 mm. Similar to holotype, except for: head narrower; first four segments of anterior tarsi slightly less dilated; dorsal setiferous punctures on pronotum 4:4, asymmetrical; abdominal sternites VII-VIII not emarginate, sternite IX with two rufous macrosetae on apex, in addition to two black ones. Variation: Length 11.9-15.6 mm. Specimens from Guerrero are mostly paler, primarily on prothorax where borders are reddish brown, and with abdominal segments VII-VIII yellow. Also, one specimen examined by Ruiz-Lizarraga (1993) and five examined by us have abdominal segments VII-VIII dark reddish brown. Aedeagi of these specimens are black, their dark color pattern is likely caused by the mixture of liquids used as killing agents. A few specimens have the pronotum and elytra apparently green in dorsal view. One specimen from Morelos has the last segment of the right maxillary palpus black. Dorsal punctures on the pronotum are highly vari- able: 2:3, 3:3, 3:4, 3:5, 4:2, 4:3, 4:4, 4:5; in some specimens, the last puncture is on the posterior half of the pronotum. The peg setae on the paramere are slightly variable in number. MATERIAL EXAMINED: Holotype: MEXICO: MORELOS, Yautepec, Carretera Mexico- Cuautla, km. 19. Selva baja caducifolia. ex excremento. 16-Jun-1996. J. Marquez col. Allotype: Tlayacapan, camino a Sta. Catarina. Selva baja caducifolia. Zona 4, 1534 msnm. 1 al 30- VI- 1996. ex NTP-80 (calamar). J. Marquez col. Paratypes: MEXICO: MORELOS, Tlayacapan. 31-X-1992. ex excremento vacuno. J. Marquez col. (ICf). Same data except for: 27-VIII-1995. I. Sanchez y J. Marquez cols. (2Cf). Same data except for: zona 5, cultivo de temporal y selva baja caducifolia. 1 al 30-VII-1995, ex NTP-80. J. Marquez col. (2Cf, 19). Same data except for: 1 al 30-IX-1996 (19). Same data except for: 1 al 30-VI-1996 (8Cf). Tlayacapan, camino a Sta. Catarina, zona 4, selva baja caducifolia. 1 -VI- 1 996, ex excremento vacuno (1 9 , ICf ). Same data except for: 9- VI- 1 996 (29, 4Cf). Same data except for: ex frutos podridos (2Cf). Same data except for: 10-VI-1996, ex excremento vacuno (ICf ). Same data except for: 1 al 30-VII-1995, ex NTP-80 (ICf). Same data exceptfor: 1 al 30-VIII- 1995 (ICf, 29 ). Same data except for: 1 al 30-IX- 1995 (29). Same data except for: 1 al 30-VI-1996 (2Cf, 29 )• Tlayacapan, San Jose de los Laureles. Bosque mesofilo de montana perturbado, zona 3. 1 al 30-X-1996, ex NTP-80. K. Villavicencio y J. Marquez cols. (19). Same data except for: 1 al 30-XI-1995 (ICf). Cuemavaca, Col. del bosque. Bosque de Pino-Encino. 23-VII-1995, ex excremento vacuno, J. Marquez col (1C?). Yautepec. Carretera Mexico-Cuautla, km. 19. Selva baja caducifolia. 16- VI- 1996, ex excremento. J. Marquez col. (ICf, 19). Tlayacapan, San Jose de los Laureles, BMM, 1751 m, 21.VII-24.VIII. 1991, J.L. Navarrete y G.A. Quiroz, # 941 D, NTP-80, (ICf, 19); same data except for: 29. VI. 1991, J.L. Navarrete, #677, ex Boletus edulis IV (ICf). GUERRERO, 2900 ft. 6 mi El Ocotito. VIII.30- IX.5-1971/ human dung trap 380. A. Newton/ yellow card. Gastrisus cf mimetes Sharp, comp. Holotype A. Newton, 1989/ Gastrisus n. sp. det. Newton 1 992 (1 Cf ). Same data: (1 9 ). 9 mi NE Iguala. 1340 m. VIII. 29-IX.4- 1971 A. Newton coll., human dung trap 378. (3Cf, ! should be consulted. Additions and corrections are given below both for the main numerically sequenced checklist of species and for the alphabetical in- dex. In the index, species names appear in italics only for the current valid form of the name as per the McCafferty ( 1 996) format. Received November 17, 1997. Accepted December 28, 1997. Department of Entomology, Purdue University, West Lafayette, IN 47907. ENT. NEWS 109(4) 266-268, September & October, 1998 Vol. 109, No. 4, September & October, 1998 267 MODIFICATIONS TO THE NUMERICAL CHECKLIST Delete 003. Choroterpes ferruginea Traver, 1934 004. Choroterpes fusca Spieth, 1938 005. Choroterpes hubbelli Berner, 1946 132. Brachyce rcus floridicola Soldan, 1986 137. Brachycercus pini Soldan, 1986 Corrections 016. Leptophlebia bradleyi Needham, 1932 363. Baetis moffatti Dodds, 1923 574. Heptagenia adaequata McDunnough, 1924 636. Rhithrogena notialis Allen & Cohen, 1977 671. Stenonema terminatum terminatum (Walsh), 1862 MODIFICATIONS TO THE ALPHABETICAL INDEX Additions Baetis curiosus (McDunnough), 1923 - 432 Baetis moffati Dodds, 1923 - 363 Baetis pallidula McDunnough, 1924 - 357 Baetis persecuta McDunnough, 1939 - 367 Blasturus cupidus (Say), 1823 - 018 Blasrurus grandis Traver, 1932 - 019 Brachycercus pallidus (Ide), 1930 - 133 Callibaetis tessellatus (Hagen), 1861 - 399 Campsurus manitobensis Ide, 1941 - 092 Cloeon chlorops McDunnough, 1923 - 355 Cloeon punctiventris McDunnough, 1923 - 371 Cloeon virilis McDunnough, 1923 - 378 Ecdyonurus fuscus (Clemens), 1913 - 672 Ecdyonurus peterseni Lestage, 1930 - 542 Ecdyonurus werestschagini (Tshernova), 1952-619 Ecdyurus hyalinus Esben-Petersen, 1916 - 542 Epeorus undulatus Banks, 1924 - 642 Ephemerella fuscata (Walker), 1853 - 179 Eurycaenis pallida Ide, 1930 - 133 Heptagenia abnorme Tshernova, 1949 - 542 Heptagenia abnormis Tshernova, 1949 - 542 Heptagenia adequata McDunnough, 1924 - 574 Heptagenia luridipennis (Burmeister), 1839 - misidentification of 667 Heptagenia verticis (Say), 1839 - 085 Heptagenia werestschagini Tshernova, 1952-619 Iron petulans Seemann, 1927 - 635 Isonychia annulata Traver, 1932 - 515 Isonychia aurea Traver, 1932-524 Isonychia thalia Traver, 1934 - 515 Leptophlebia concinna (Walker), 1853 - 018 Leptophlebia hebes (Walker), 1853 - 018 Leptophlebia ignava (Hagen), 1861 - 018 Leptophlebia pallipes (Walker), 1853 - 018 Oreianthus sp. 1 Traver, 1937 - 126 Paraleptophlebia compar Traver, 1934 - 033 Paraleptophlebia pallipes (Hagen), 1875 - 049 Potamanthus rufus Argo, 1927 - 082 Pseudocloeon virilis (McDunnough), 1923 - 378 Rhithrogena fusca (Walker), 1853 - 634 Rhithrogena imanica Bajkova, 1972 - 619 Siphlonurus bernice McDunnough, 1923 - 509 Siphloplecton basalis (Walker), 1853 - 477 Stenonema Carolina (Banks), 1914 - 646 Stenonema tessellata (Walker), 1853 - 672 Corrections Ameletus mantis Mayo, 1952 - 313 Baetis moffatti Dodds, 1923 - 363 Brachycercus floridicola Soldan, 1986 - 129 Brachycercus pini Solda"n, 1986 - 134 Brachycercus sp. A Berner, 1950 - 129 Choroterpes ferruginea Traver, 1934 - 002 Choroterpes fusca Spieth, 1938 - 002 Choroterpes hubbelli Berner, 1946 - 002 Cinygma deceptiva McDunnough, 1924 - 555 Cloe mollis Hagen, 1861 - 051 Cloeon vicinum Hagen, 1861 - 471 Epeorus humeralis Morgan, 1911 - 573 Heptagenia adaequata McDunnough, 1924 - 574 Leptophlebia bradleyi Needham, 1932 - 016 Paraleptophlebia bradleyi (Needham), 1932 - 016 Pseudocloeon Carolina (Banks), 1924 - 335 Pseudocloeon cingulatum McDunnough, 1931 - 353 Rhithrogena hespera Banks, 1924 - 561 Rhithrogena notialis Allen & Cohen, 1977-636 Rhithrogena petulans (Seemann), 1927 - 635 Thraulus albertanus McDunnough, 1931 - 077 268 ENTOMOLOGICAL NEWS ACKNOWLEDGMENTS I would like to thank Manny Pescador (Tallahassee, Florida), Bob Waltz (Indianapolis, Indi- ana), and Boris Kondratieff (Fort Collins, Colorado) for calling my attention to certain informa- tion. This paper has been assigned Purdue University ARP No. 15537. LITERATURE CITED McCafferty, W. P. 1996. The Ephemeroptera species of North America and index to their com- plete nomenclature. Trans. Am. Entomol. Soc. 122: 1-54. McCafferty, W. P. 1997a. Ephemeroptera. Pages 89-117. In: R. E. Poole and P. Gentili (eds.). Nomina Insecta Nearctica, a checklist of the insects of North America, Volume 4: Non-ho- lometabolous orders. Entomological Information Services, Rockville, Maryland. McCafferty, W. P. 1997b. Name adjustments and a new synonym for North American Ephe- meroptera species. Entomol. News 108: 318, 320. Zloty, J. 1996. A revision of the Nearctic Ameletus mayflies based on adult males, with descrip- tions of seven new species (Ephemeroptera: Ameletidae). Can. Entomol. 128: 293-346. SCIENTIFIC NOTE ADDITIONS TO IOWA MAYFLIES (EPHEMEROPTERA)1'2 W. P. McCafferty3, Todd Hubbard4 Klubertanz (1995) recorded 66 nominal species of mayflies from the state of Iowa. Thirty-nine of those records represented new state records. Herein we add 10 new Iowa records. Newly reported materials are deposited at the Purdue Entomological Research Collection, West Lafayette, IN (PERC) or the University of Iowa Hygienic Laboratory, Des Moines, I A (IHL). New state records include Baetisca obesa (Say) [larvae, Black Hawk Co, falls access, VI-9-1973 (PERC)]; Homoeoneuria ammophila (Spieth) [larvae, Ida Co, Maple R, VIII-5-1997 (IHL)]; Labiobaelis dardanus (McDunnough) [larvae, Ida Co, Odebolt Cr, VIII-5-1997 (IHL)]; Leucrocuta juno (McDunnough) [adults, Story Co, Ames, VIII-1-1991 (PERC)]; Procloeon irrubrum Lowen and Flannagan [all larvae, all IHL: Dickinson Co, Little Sioux R, 1X-4-1996; Webster Co, Lizard Cr, IX- 1 1 - 1 996; Kossuth Co, Black Cat Cr, VIII- 1 3- 1 997; Shelby Co, West Nishnabotna R, VIII- 15-1 997; Warren Co, Whitebreast Cr, VIII- 1 - 1 997]; Procloeon rufostrigatum (McDunnough) [larvae, Webster Co, Lizard Cr, IX-1 1-1996 (IHL)]; Procloeon viridoculare (Berner) [all larvae: Buchanan Co, Bear Cr, VIII-8-1996; Winnishiek Co, Canoe Cr, IX-9-1997 (IHL)]; Rhithrogena jejuna Eaton [larvae, Winnishiek Co, Upper Iowa R nr Kendallville, IX-22-1991 (PERC)]; Stenacron candidum (Traver) [adults, Black Hawk Co, Cedar Falls, VII-1-1973 (PERC)]; and Stenacron Carolina (Banks) [adults, Black Hawk Co, Cedar Falls, VI-10- 1973 (PERC)]. LITERATURE CITED Klubertanz, T. H. 1995. Survey of Iowa mayflies (Ephemeroptera). J. Kans. Entomol. Soc. 68: 20-26. 1 Received April 3, 1998. Accepted April 4, 1998. 2 Purdue Agricultural Research Program Journal No. 15699. 3 Department of Entomology, Purdue University, West Lafayette, IN 47907. 4 Hygienic Laboratory, University of Iowa, H. A. Wallace Bldg., Des Moines, I A 50319. ENT. NEWS 109(4) 268, September & October, 1998 Vol. 109, No. 4, September & October, 1998 269 TAXONOMIC NOTES ON EVANIODINI (HYMENOPTERA: BRACONIDAE), WITH REDESCRIPTION OF EVANIODES SPATHIIFORMIS AND DESCRIPTION OF A NEW SPECIES1 S. M. Barbalho2, A. M. Penteado-Dias3 ABSTRACT: .The monobasic Pariodes (Hymenoptera: Braconidae, Doryctinae, Evaniodini) is synonymized with Evaniodes Szepligeti, 1901, thus P. spathiiformis Szepligeti, 1901 is returned to Evaniodes. Redescription and illustrations of male and female Evaniodes spathiiformis are provided as well as a description of a new species, Evaniodes marshi. The tribe Evaniodini (Hymenoptera: Braconidae, Doryctinae) was created by Fischer (1981) for two species described by Szepligeti (1901) from Brazil, and characterized by the elevation of the metasoma above the hind coxa. One of these species, Evaniodes areolatus Szepligeti, is known only from the fe- male while the second species, E. spathiiformis Szepligeti, is known from the male and female. Szepligeti (1901 ) differentiated the two species primarily by the shape of the first metasomal tergite (Tl) (4 times longer than wide in spathiiformis; 3 times longer than wide in spathiiformis) and by color (spathiiformis reddish-yellow with black head and antenna and bicolored wings; areolatus more extensively black with wings almost hyaline). Roman (1924) noted that both sexes of E. spathiiformis had a closed 1st subdiscal cell in the fore wing and lighter body color; the female was larger and the male had a more compact hind wing venation illustrated by him as from spathiipennis. He also described areolatus as being smaller than spathiiformis; with body color black with reddish-brown areas and the fore wing brachial cell more or less open. Because of the pronounxced differences between the two species, Fischer (1981) described a new genus, Pariodes, with spathiiformis (male) as its type and only included species. He did not mention the female used by Roman (1924). His redescription of E. areolatus indicates that the propodeum has a pentagonal cell and the hind wing has more complete venation compared to P. spathiiformis. Differences between the two include eye height in comparison to temporal height; clypeus height; notauli (rounded or straight, deep or not deep); venation of hind wing; fore wing 1st subdiscal cell open in E. areolatus and closed in P. spathiiformis; size of fore wing; Tl height versus apical width and size of the body. 1 Received June 27, 1997. Accepted December 2, 1997. 2 Programa de Pos-Graduacao em Genetica e Evoluc.ao, Universidade Federal de Sao Carlos, CP 676, CEP 13 565-905, Sao Carlos, SP, Brasil. 3 Departamento de Ecologia e Biologia Evolutiva, Universidade Federal de Sao Carlos, CP676, CEP 13 565-905, Sao Carlos.SP, Brasil. ENT. NEWS 109(4) 269-273, September & October, 1998 270 ENTOMOLOGICAL NEWS Belokobyl'skiy (1993) also treated these two species as being valid for the tribe Evaniodini and shows the following characters for the tribe: elevated abdomen; thorax short and swollen; notauli complete; sternauli not developed; propodeum with or without areola; fore wing 1st subdiscal cell closed; hind coxa with an antero-ventral basal tubercle; Tl pedicellate and T2 and T3 smooth. Marsh (1993) incorrectly included both E. areolatus and P. spathiiformis in the group of Braconidae with no antero-ventral basal tubercle on the hind coxa. Based on the study of male and female specimens of E. spathiiformis and the male of a new species from Brazil, we propose to synonymize Pariodes Fischer with Evaniodes Szepligeti, thus returning spathiiformis to the genus Evaniodes. We compared male and female Evaniodes specimens with the de- scriptions in the literature and found the only difference between them was in the hind wing venation (Figs 1 ,2) with M+CU and cu-a meeting at junction of SC+R1, Rl, SR and 2-M, with 1-M and r-m absent in the male and veins M+CU, 1-m and Ir-m distinct, SC+R1 reaching Rl before the middle of the wing in the female. For the morphological terminology used in this paper, see van Achterberg (1993).The following abbreviations are used to indicate deposition of speci- mens: (INPA), Institute Nacional de Pesquisas da Amazonia, Brasil, (INPA), Institute Nacional de Pesquisas da Amazonia, Brasil, (MPEG), Museu Paraense Emelio Goeldi, Brasil, ( DCBU) Departamento de Ecologia e BiologiaEvolutiva da Universidade Federal de Sao Carlos, Brasil. Evaniodes spathiiformis Szepligeti, 1901 (Figs 1-4) Female Head.- 2.3 times wider than long, 1 .5 times wider than mesonotum; occipital carina present; gena smooth, face height and width equal, possessing transverse striation; clypeus transversely striated, 2.5 times broader than height, rounded ventrally; face as broad as basal width of man- dible; very large eyes occupying most of head; in lateral view, eye height 1 .35 times greater than length and 3.6 times longer than gena; antenna length shorter than body length; antennae with 46 antennomeres; first flagellomere length 1 .7 times longer than second, succeeding antennomeres gradually shorter and thinner, placodes evident. Mesosoma.- Length 1.2 times longer than width; mesonotum 1.3 times wider than long; tegula trapezoidal; deep and light-crenulate notauli touching each other in rugose area at base of mesonotum, with accompanying sparse hairs; scutellar sulcus with 5 cross carinae; scutellum smooth with setae in apical area; propodeum smooth with no pentagonal areola and sparsely setose; pronotum smooth with posterior sulcus; mesopleuron smooth; sternaulus not deep, epicnemial carina present extending to medium area of mesopleuron; metapleuron with sparse hairs; metasternum large and long; hind coxa with weakly distinct antero-ventral basal tubercle; first tarsomere about 2 times longer than second, second 1 .2 times longer than third plus fourth, fourth 2 times longer than third, fifth 2.5 times longer than fourth; apical tibial spurs short ( 1/6 of basitarsus) (Fig. 1). Fore wings.- Not reaching end of metasoma; 6.4 mm in length; stigma moderately broad , 6.7 times longer than vein r; r almost as long as stigma width; 3-SR length 1 .4 times longer than 2- SR vein; SRI straight and 2.5 times longer than 3-SR; SRI vein reaching end of wing; m-cu Vol. 109, No. 4, September & October, 1998 271 straight and interstitial; 2b cell square-shaped; 2-CU1 length 2.8 times longer than m-cu; cu-a vein postfurcal; 4a cell closed at apex, vein CUlb present and distinctly meeting 2-1 A; CUla vein arising below middle of 4a cell (Fig. 2). Hind wings.- Veins M+CU, 1-M and Ir-m distinct; SC+R1 reaching Rl before middle of wing; M+CU as long as 1 -M; 1 -M 2 times longer than m-cu; m-cu almost reaching posterior side of the wing (Fig. 3). Metasoma.- Inserted high on the propodeum, distance between insertion of metasoma and hind coxa about equal in length to hind coxa; Tl long and thin, 3.3 times longer than apical width, apical width slightly longer than basal one; Tl striated only at apex; T2 striated and wider than long as well as remaining tergite; T3 striated at base and rugose at apex; remaining tergites smooth. Ovipositor.-Much longer than body length. Color.- Head black; mandibles yellow with apical area black; yellow palps; antennae brown; thorax fully yellowish; legs brown (only mesocoxa is yellow); metasoma with basal third light brown and apical area black; wing membrane infuscated, stigma and veins yellow. Variation in female. -Head black with dark-brown gena and occiput; eye height greater (varying between 3.4 to 3.6) than gena; notauli very deep; 2 sulci in pronotum, dorsal smooth, ventral crenulated; apical width of Tl varying between 3.0 to 3.2 times longer than basal width; T2 and T3 fully striated with no rugose area; body length between 7.0 and 8.9 mm. Variation in male.- Clypeus 1.7 to 2.25 broader than its height; eye height 2.3 to 3.1 longer than gena, 2.3 to 3. 1 ; wings (Fig. 4) slightly longer than body, between 6.4 to 7. 1 mm; in hind wing veins 1 -M and r-m absent, M+CU and cu-a meeting at junction of SC+R 1 , R 1 , SR and 2-M; T 1 3.8 to 4.3 times longer than its apical width; body length 6.0 to 7.4 mm. Distribution.- Known only from Brazil : Belem (Para State), Manaus (Amazonas State), and Mato Grosso State. Material examined. 1 female (INPA), "Est. Cemat. R. Humb. MT, 26.IX.1975"; 1 female (MPEG), "Amazonas, Manaus, 1 Km W Taruma, Falls, 14.11.1981, 100 m G. Okis, primary for- est"; 1 female ( DCBU), "Reserva Ducke, Manaus, Amazonas, E.V.Silva & A. Faustino, 3. V. 1968", 1 male (INPA), "Est. Am 1, Km 101, Mn.Am., Brasil, V. 1968, col. several"; 1 male (MPEG) "Brasil, Pa, Belem, Mocambo, 31. VIII. 1986"; 1 male (DCBU) , "F. Esteio, Res. 1112, Manaus, ZF3, Km 23, B. Klein, col., 3. 1. 1986". Evaniodes marshi Barbalho & Penteado-Dias SPEC. NOV. (Fig. 5) Male Head. - with many hairs; 1.5 times longer than wide, 1.5 times wider than mesonotum; occipital carina present; gena smooth, face height and width equal, possessing transverse striation; clypeus transversely striated, as broad as long , straight ventrally; face as broad as mandibular base width; very large eyes occupying most of head; in lateral view, eye height 1.2 times greater than length and 3.6 times longer than gena; first flagellomere length 1.9 times longer than second, succeed- ing antennomeres gradually shorter and thinner. Mesosoma.- Length 1.6 times longer than wide; tegula elliptical; shallow and light-crenulate notauli not touching each other at base of mesonotum, with accompanying sparse hairs; mesoscutum smooth and shining; scutellum smooth with setae in the apical area; propodeum rugose with no pentagonal areola and with many hairs; mesopleuron smooth , with many hairs, as well as middle coxae; sternaulus absent, epicnemial carina present extending to medium area of mesopleuron; metapleuron with many hairs; hind coxa without an antero-ventral basal tu- bercle; first tarsomere about 2 times longer than second, second as long as third plus fourth, fourth 2.4 times shorter than third, fifth 2.1 times longer than fourth; apical tibial spurs short (I/ 6 of basitarsus); hind femora with a large blister like swelling anteriorly near base (Fig. 5). 272 ENTOMOLOGICAL NEWS .2-SC+R Mi-CU 1r-,m( fi 1^+R_Z_1X51 cu-a Figures 1- 4. Evaniodes spathiiformis .1, female, hind tarsus; 2, female, fore wing; 3, female, hind wing; 4, male, hind wing. Figure 5. Evaniodes marshi spec. nov. .male, hind leg. Vol. 109, No. 4, September & October, 1998 273 Fore wings.-Not reaching end of metasoma; 5.7 mm in length; stigma moderately broad , 4.6 times longer than vein r; r shorter than stigma width; 3-SR as long as 2-SR vein; SR 1 straight and 2.5 times longer than 3-SR; SR 1 vein reaching end of wing; m-cu straight and joining 2M before 2 RS; 2b cell square-shaped; 2-CU 1 length 2.6 times longer than m-cu; cu-a vein postfurcal; first subdiscoidal cell closed at apex, vein CUlb present and distinctly meeting 2-1 A; CUla vein arising below middle of 4a cell . Hind wings.- Veins 1 -M and r-m absent, M+CU and cu-a meeting at junction of SC+R 1 , R 1 ,SR and 2-M. Metasoma.- Inserted high on propodeum, distance between insertion of metasoma and middle coxa about equal in length to middle coxa; Tl long and thin, 2.7 times longer than apical width, apical width slightly longer than basal width; Tl striated only at apex; T2 striated and wider than long ; T3 striated; remaining tergites smooth. Color.- Head black; mandibles yellow with apical area black; yellow palps; antennae black; thorax fully black; fore and middle legs yellow (except middle coxa brown), hind legs dark brown; metasoma brown; wing membrane lightly infuscated, stigma and veins brown. Material examined.- Holotype, Brazil, Amazonas, Jurua, Mineruazinho. 1 male , 25/1/96 (depos- ited in INPA). Female unknown. Etymology.- Named for Paul M. Marsh because of his contributions to the knowledge of Doryctinae wasps . Key to species of Evaniodes. la- Propodeum with a pentagonal cell; 1st subdiscal cell open E. areolatus Szepligeti Ib- Propodeum without a pentagonal cell; 1st subdiscal cell closed 2. 2a- Body fully black and with many hairs; hind femora with a blister like swelling anteriorly near base (known only from male) E. marshi spec, nov 2b- Body fully yellow and with sparse hairs; hind femora without a blister anteriorly near base E. spathiiformis Szepligeti LITERATURE CITED Achterberg, C. van 1993. Illustrated key to the subfamilies of the Braconidae (Hymenoptera: Ichneumonoidea). Zool. Verb. Leiden 283: 1-189, figs 1-66, photos 1-140, plates 1-102. Belokobyl'skiy, S. A. 1993. On the classification and phylogeny of braconid wasps of the sub- family Doryctinae and Exothecinae (Hymenoptera, Braconidae). Part 1. On the Classifica- tion, l.Entomol. Rev, 77: 109-137. Fischer, M. 1981.Versuch einer systematischen Gliederung der Doryctinae, insbesondere der Doryctini, und Redeskriptionen nach Material aus dem Naturwissenschaftlichen Museum in Budapest (Hymenoptera, Braconidae). Polskie Pismo Entomol., 51: 41-99. Marsh, P. M. 1993. Description of new Western Hemisphere genera of the subfamily Doryctinae (Hymenoptera, Braconidae). Contrib. Amer. Entomol. Instil., 28 (1): 1-58. Roman, A. 1 924. Wissenshaftliche Ergebnisse der schwedischen entomologishen Reise des Herrn Dr. A. Roman in Amazonas. 1914-1915. Hymenoptera: Braconidae, Cyclostomi pro p. Arkiv Fur Zool., 20(16): 1-40. Szepligeti, G. V. 1901. Tropische Cenocoelioniden und Braconiden aus der Sammlung des Ungarischen National-Museums. Term. Fiiz., 24: 353-405. 274 ENTOMOLOGICAL NEWS ZORCADWM BERGROTH, AN OBJECTIVE JUNIOR SYNONYM OF PSEUDOBEBAEUS FALLOU (HETEROPTERA: PENTATOMIDAE)1 D. A. Rider2, Christian Fischer' ABSTRACT: Zorcadium Bergroth, 1918, is placed as an objective junior synonym of Pseudobebaeus Distant, 1911 based on study of their respective type species, Pseudobebaeus goyazensis Distant, 1911, a junior synonym of Euschistus truncatus Fallou, 1 888. While working on a catalog of the Pentatomidae of the world, a number of nomenclatural problems have been discovered. Many of these problems have already been corrected (Rider & Rolston 1995, Rider 1997). The problem dealt with in this paper concerns two monotypic genera that were at one time placed in separate families, but their respective type species are here shown to be conspecific. Pseudobebaeus Distant, 1911 Pseudobebaeus Distant, 1911: 254; Kumar, 1974: 20; Fischer, 1996: 83-87. Type species: Pseudobebaeus goyazensis Distant, 191 1, by monotypy. Zorcadium Bergroth, 1918: 307-308; Rolston & McDonald, 1981: 259, 269; Rider, 1994: 194, 217-218. Type species: Euschistus truncatus Fallou, 1888, by monotypy. New Synonymy. Description: See Rolston & McDonald (1981) and Rider (1994) for de- tailed descriptions of this taxon under the name Zorcadium. Also, see Fischer (1996) for descriptive notes on Pseudobebaeus. Pseudobebaeus truncatus (Fallou, 1888), NEW COMBINATION Euschistus truncatus Fallou, 1888: 36. Euschistus trancatus [sic]: Bergroth, 1892: 263. Thoreyella truncata: Lethierry & Severin, 1893: 176; Kirkaldy, 1909: 137. Pseudobebaeus goyazensis Distant, 1911: 255; Fischer, 1996: 83-87, figs. 1 -6. New Synonymy. Zorcadium truncatum: Bergroth, 1918: 308; Rolston & McDonald, 1981: 269, 270, figs. 32-39; Rider, 1994: 218, figs. 136-148. Description: See Rolston & McDonald (1981) for detailed description under the name Zorcadium truncatum, and Fischer ( 1 996), for descriptive notes under the name Pseudobebaeus goyazensis. 1 Received September 23, 1997. Accepted February 9, 1998. 2 Department of Entomology, Box 5346, University Station, North Dakota State University, Fargo, North Dakota 58105, U.S.A. 3 Freie Universitat Berlin, Institut fur Zoologie, AG Entomologie, Konigin-Luise-Str. 1-3, D- 14195 Berlin, Germany. ENT. NEWS 109(4) 274-276, September & October, 1998 Vol. 109, No. 4, September & October, 1998 275 Distribution: Brazil, Peru. Specimens Examined: 20*0", 39 9. 1 O", Brazil, UZMH; ICf, Brazil: MatoGrosso: 10°25' S, 59°28' W, 1 7-22-III, DAR; 1 9 , Brazil: Goias [lectotype of Pseudobebaeus goyazensis], BMNH; 1 9 PERU: Andres AvelinoCaceres: Satipo, 10-VIII, USNM; 1 9 , Peru: Andres AvelinoCaceres: Tingo Maria 1km E of town, forested eastern foothills of the Andes, 5- VIII, BMNH. DISCUSSION Fallou (1888) described Euschistus truncatus from Minas Gerais, Brazil. Although this species is brown and superficially resembles other species of Euschistus Dallas, Bergroth (1892) suggested that it probably belonged in a new genus related to Thoreyella Spinola. He later (1918) described Zorcadium to hold this single species. This is the position in which the senior author (DAR) treated this taxon in his (Rider 1994) conspectus of the tribe Procleticini. Distant (1911) described Pseudobebaeus goyazensis apparently from a single female specimen from Goias, Brazil. Although Distant did not specifi- cally state that this species belonged with the acanthosomatids, it is obvious that this was his intended placement. His paper does not contain any subhead- ings below Pentatomidae; it does treat (in order) scutellerids, pentatomids (pentatomines), pentatomids (asopines), and acanthosomatids. He placed Pseudobebaeus between the asopines and the acanthosomatids and stated that it was "allied to Bebaeus, Dall., in general appearance and structure of head and lateral pronotal angles; ..." Bebaeus Dallas is properly a member of the Acanthosomatidae. Kumar (1974) followed Distant in placing this species in the family Acanthosomatidae and further placed it in the Blaudusinae: Lanopini. The junior author (CF), after examining the female lectotype, presented con- vincing evidence that this species did not belong in the Acanthosomatidae (Fischer 1996), but its specific placement within the Pentatomidae remained a mystery. DAR, while reviewing the above paper by CF, noticed that the illustrations of Pseudobebaeus goyazensis were similar to his own illustrations of Zorcadium truncatum presented in the procleticine conspectus. Further discussion, and a re-examination by DAR of the lectotype of Pseudobebaeus goyazensis con- firmed that the two species were conspecific. Although the holotype of Euschistus truncatus is apparently lost (Rolston and McDonald 1981 ), its iden- tity has not been in question; a voucher specimen designated by Rolston and McDonald ( 1 98 1 ) was examined. Pseudobebaeus has priority over Zorcadium, but P. goyazensis will fall as a junior synonym of Z. truncatum. Thus the proper combination is Pseudobebaeus truncatus (Fallou, 1888). Its proper placement is within the tribe Procleticini in the Pentatomidae. Note: The first line on p. 217 of the diagnosis for Zorcadium in Rider (1994) should read "Juga contiguous anteriorly; ..." with the "not" omitted. 276 ENTOMOLOGICAL NEWS ACKNOWLEDGMENTS We thank the following individuals for their help in lending specimens pertinent to this project (acronyms are those used in the text; DAR is senior author's collection): Janet Margerison- Knight, British Museum (Natural History), BMNH; T. J. Henry, Systematic Entomology Labora- tory, USDA, c/oUnited States National Museum of Natural History, USNM; A. Jansson, Zoo- logical Museum, University of Helsinki, UZMH. We also thank J. E. Eger, DowElanco, Tampa, FL, and L. H. Rolston, Baton Rouge, LA, for their reviews of an early draft of the manuscript. LITERATURE CITED Bergroth, E. 1892. Notes synonymiques. Revue d'Entomol. 1 1:262-264. Bergroth, E. 1918. Hendecas generum Hemipterorum novorum vel subnovorum. Ann. Musei Nationalis Hungarici 16:298-314. Distant, W. L. 1911. Rhynchotal notes. 53 Neotropical Pentatomidae. Ann. Mag. Nat. Hist. (8)7:242-258. Fallou, J. 1888. Hemipteres nouveaux recueilles a Minas Geraes. Le Naturaliste (2)1:36. Fischer, C. 1996. On the systematic position of Pseudobebaeus goyazensis Distant, 1911 within the Pentatomoidea (Heteroptera, Pentatomoidea). Deutsche Entomol. Zeitschrift 43(1): 83- 87. Kirkaldy, G. W. 1909. Catalogue of the Hemiptera (Heteroptera) with biological and anatomi- cal references, lists of foodplants and parasites, etc. Vol. I. Cimicidae. Berlin, xl + 392 pp. Kumar, R. 1974. A revision of world Acanthosomatidae (Heteroptera: Pentatomidae): Keys to and descriptions of subfamilies, tribes, and genera, with the designation of types. Austr. J. Zool., supplement 34: 1-60. Lethierry, L. and G. Severin. 1 893. Catalogue general des Hemipteres. Bruxelles, Pentatomidae, 1: x + 286 pp. Rider, D. A. 1994. A generic conspectus of the tribe Procleticini Pennington (Heteroptera, Pentatomidae), with the description of Parodmalea rubella, new genus and species. J. N. Y. Entomol. Soc. 102(2): 193-221. Rider, D. A. 1997. Rolstoniellini, replacement name proposed for Compastini Distant, 1902, a tribal name based on a generic junior homonym (Heteroptera: Pentatomidae: Pentatominae). J. N. Y. Entomol. Soc. 103(4):40 1-403. Rider, D. A. and L. H. Rolston. 1995. Nomenclatural changes in the Pentatomidae (Hemi- ptera-Heteroptera). Proc. Entomol. Soc. Wash. 97(4):845-855. Rolston, L. H. and F. J. D. McDonald. 1981. Conspectus of Pentatomini genera of the western hemisphere - part 2 (Hemiptera: Pentatomidae). J. N. Y. Entomol. Soc. 88(4)[1980]:257- 272. ANNOUNCEMENT After a number of years without an editor, the Memoirs of the American Entomological Society is now under the stewardship of Norman E. Woodley. The Society is therefore eager to continue publication of this long-standing monographic series. Potential manuscripts or propos- als for submissions are welcome at any time. Correspondence about manuscripts should be sent to: Norman E. Woodley Systematic Entomology Lab-USDA c/o Smithsonian Institution NHB-168 Washington, DC 20560-0168 Memoirs are normally taxonomic or faunistic in subject matter. Manuscripts and plates should be substantial enough to produce a minimum of 75 printed pages. Authors will be expected to have funding to pay for a substantial portion of the cost of publication. Vol. 109, No. 4, September & October, 1998 277 MASS APPEARANCE OF LADY BEETLES (COLEOPTERA: COCCINELLIDAE) ON NORTH CAROLINA BEACHES1 C. A. Nalepa2, K. R. Ahlstrom2, B. A. Nault3, J. L. Williams4 ABSTRACT: A mass appearance of lady beetles on the North Carolina coast was investigated in May of 1996. Six species of lady beetles were identified, with Hippodamia convergens and Coccinella septempunctata predominating. It is suggested that the insects were first-generation adults dispersing from senescing grain fields. Several publications document the sudden appearance of hordes of lady beetles (Coccinellidae) on the beaches of oceans and large lakes (Oliver, 1943; Hagen, 1962; Rothschild, 1971;Yanet al., 1983; Majerus and Majerus, 1996). These sporadic mass appearances are not associated with dormancy or aggre- gation and are usually attributed to the weather. Wind patterns concentrate masses of flying beetles and drop them into bodies of water; large numbers of beetles subsequently wash up on beaches as the result of wind and tides. The number of beetles involved can be staggering. Oliver (1943), for example, described a drift line of dead Coccinella undecimpunctata L. at least 13 miles long with 70,000 beetles per linear foot. In the United States the phenomenon has been reported by Lee (1980) in the Great Lakes of the upper midwest, and by Schaefer et al. (1987) along the coast of Delaware. Hagen (1962) reported that masses of Hippodamia convergens Guerin-Meneville are occasionally deposited in the Pacific Ocean. We had the opportunity to investigate reports of a large number of coccinellids washed up on a beach in the city of Kitty Hawk (36.07°N, 75.72°W) on one of North Carolina's barrier islands. Local residents reported that the lady beetles arrived in large numbers on 18 May 1996. On 25 May 1996 we collected and identified 919 insects from debris east of the primary dune. Of these, 96% were predaceous coccinellids, 3% were other Coleoptera, and 1% were assorted Hemiptera and Diptera. Seven percent of the insects were alive when collected, and all but the Diptera were identified to species (Table 1 ). Six species of lady beetles were collected, with Hippodamia convergens and 1 Received November 8, 1997. Accepted January 22, 1998. 2 Beneficial Insects Laboratory, North Carolina Department of Agriculture, P.O. Box 27647, Raleigh, NC 27611. 3 Eastern Shore Agricultural Research and Extension Center, Virginia Polytechnic Institute and State University, 33446 Research Drive, Painter, VA 23420-2827. 4 Planning and Environmental Branch, North Carolina Department of Transportation, P.O. Box 25201, Raleigh, NC 27611. ENT. NEWS 109(4) 277-28 1 , September & October, 1998 278 ENTOMOLOGICAL NEWS Coccinella septempunctata L. dominating (55.8% and 41 .5% of Coccinellidae collected, respectively). To determine if this mass appearance was a localized anomaly, one week later (1 June 1996) we visited Wrightsville Beach, NC (34.2 1°N, 77.80°W), 281 km south of Kitty Hawk. Seventy-two dead beetles were collected in de- bris at this location: H. convergens (51.4%), C. septempunctata (44.4%), C. munda (2.8%), and C. maculata lengi (1.4%). The three non-coccinellids col- lected were identified as Chrysomela scripta (Fabricius). These beetles were all dead and infrequently encountered. We suspect that the insects collected at both Kitty Hawk and Wrightsville Beach were remnants of the same phenom- enon, but most of the beetles at the latter location had washed or blown away by the time we visited. The species collected at Kitty Hawk are a common assemblage of lady beetles in North Carolina and are reported in varying proportions from crops and ornamental plantings (Kidd, 1996; Nault, unpublished data; Nalepa, un- published data). All are aphidophagous to varying degrees, conforming to the prevailing hypothesis that mass appearances of lady beetles on beaches are related to the nature of their aphid diet (Hodek, 1973; Hodek et al., 1993). Aphids rapidly increase in number under favorable conditions, but this abun- dance is sporadic and ephemeral in most habitats (Hodek, 1973). Aphido- phagous lady beetles, in turn, have evolved two traits that predispose them to tracking prey of this nature. First, they are able to respond to an abundance of prey with spectacular increases in population size (Hagen, 1962; Hodek, 1973; Hodek and Honek, 1996; Majerus and Majerus, 1996). Dickson et al. (1955), for example, estimated that nearly 54,000 adult coccinellids emerged from one acre of alfalfa heavily infested by aphids. Second, aphidophagous lady beetles are more nomadic than species that use other food sources, and may switch among several habitats with suitable prey during one vegetational sea- son. They are especially prone to fly when hungry (Ewert and Chiang, 1 966a,b; Hodek et al., 1993; Hodek and Honek, 1996; Majerus and Majerus, 1996). In explaining the mass shoreline appearances of aphidophagous coccinellids, Hagen (1962) proposed a plausible chain of events subsequently echoed and endorsed by other authors (Hodek, 1973; Hodek and Honek, 1996; Majerus and Majerus, 1996). Favorable environmental conditions, i.e., massive aphid populations and optimal weather, allow for a high fecundity of female coccinellids and a low mortality of larvae and pupae. Juvenile popula- tions build quickly, and when the young adult beetles of this generation emerge, there is stiff competition for remaining prey. Hunger increases their mobility, and a hot day may bring them into the air by the millions; beetles in flight may be further concentrated by thermals and prevailing winds. The insects are brought back to earth en masse by air currents at the coast and perhaps a reluc- tance to cross expanses of water. Those that land in water are washed back onto the coast by wave action and tides. Vol. 109, No. 4, September & October, 1998 279 Hodek and Honek (1996) consider the species composition of these mass appearances purely accidental "pseudo-communities" that may not resemble coccinellid communities of any habitat in the vicinity; they cite Klausnitzer's (1989, 1992) work on the German coast of the Baltic Sea. This researcher compared relative abundance of coccinellid species from seashore collections with those present in nearby pine forests and found little correlation. The tim- ing and species composition of the mass appearance of coccinellids on North Carolina beaches in 1996, however, suggests the possibility that these origi- nated in grain fields prevalent in the eastern half of the state. First, nearly 700,000 acres of small grains were harvested in this area of North Carolina during 1995; harvest typically begins in late May and early June (Meadows, 1996). Second, adults of the first generation of coccinellids emerge in late May, at about the same time grain is senescing (Kidd, 1996; Nault, unpub- lished data). Third, over most of North Carolina prevailing winds near the earth's surface blow from the southwest. The direction may be interrupted and reversed due to offshore storms or diurnal fluctuations (Hardy et al., 1967). Fourth, two of the major species comprising the beach population were also abundant in nearby grain fields. Lady beetles swept from wheat at the Tidewa- ter Research Station near Plymouth in Washington County on 3 May 1996 consisted of 38.3% C. septempunctata, 30.4% H. convergent, and 31.3% C. maculata (n = 240) (Kidd, 1996). The presence of C. septempunctata and H. convergent at the beach is not difficult to explain. C. septempunctata is primarily an aphid predator (Gordon, 1985) prone to population explosions (Hodek and Hon£k, 1996; Majerus and Majerus, 1996), is a strong flier (Marriner, 1939), and is the dominant species collected from mass aggregations on coastlines (Rothchild, 1971; Van et al., 1983; Schaefer et al., 1987). During the breeding period, the most important movements of C. septempunctata in Europe occur after aphids disappear from cereal stands, when the emergence of new adults more or less coincides with a decline in aphid populations in the fields (Hodek and Honek, 1996). H. convergent represented a higher proportion (55.8%) of our beach collection than has been reported in the past. In the coastal collection described by Schaefer et al. (1987), for example, just 5.3% were identified as H. convergent. This coccinellid is strictly aphidophagous, and can be the most abundant species present in cereals (Gordon, 1985; Hodek and Hon£k, 1996: Table 5.16). If the lady beetles that appeared on the North Carolina coast in 1996 indeed origi- nated from small grain, then Coleomegilla maculata is conspicuous by its rela- tive absence from the beach. Although its scarcity might be due to variation in demographic parameters (i.e., adults of the first generations of C. septem- punctata and H. convergent may have emerged and flown while C. maculata were still pupae), we think a better explanation lies in host range differences among species. While the primary food source of C. septempunctata and H. convergent is aphids, C. maculata is perhaps the most polyphagous lady beetle 280 ENTOMOLOGICAL NEWS known, feeding on aphids, other insect prey, insect eggs, fungi, and pollen (Hodek, 1973; Hilbeck and Kennedy, 1996). Up to 50% of the diet of C. maculata can be composed of pollen from various plants (Forbes, 1883). As such, the life history of this species is not strongly tied to aphid demographics (Ewert and Chiang, 1966b), and it is less prone to long distance movements (Hodek and Honek, 1996). After the collapse of aphid populations in small grain, new adults of C. maculata can support themselves on nearby alternative food instead of undertaking a risky dispersal flight in search of aphid prey. Voucher specimens have been deposited in the North Carolina Department of Agriculture Insect Collection, Raleigh. Table 1. Insect species collected from beach debris at Kitty Hawk, North Carolina, on 25 May 1996 (n = 919 insects; 7 Diptera were not identified). Family Species No. Coccinellidae Hippodamia convergens Guerin-Meneville 493 Coccinella seplempunctata L. 367 Cycloneda nninda (Say) 15 Harmonia axyridis (Pallas) 6 Coleomegilla maculata lengi Timberlake 2 Anatis labiculata (Say) 1 Scarabaeidae Macrodactylus anguslatus (Beauvois) 3 Chrysomelidae Diabrotica undecimpunctata howardi Barber 8 Chrysomela (Microdera) scripla (Fabricius) 7 Leptinotarsa decemlineata (Say) 5 Calligrapha (Coreopsomela) californica 2 coreopsivora Brown Saldidae Saldula major (Provancher) 1 Pentatomidae Neottiglossa (Texas) cavifrons Stal 1 Cydnidae Sehirus cinctus (Beauvois) 1 ACKNOWLEDGMENTS We thank Robin Goodson and George Kennedy for commenting on the manuscript, and alert citizen Jenny Rand for reporting the coastal coccinellid convergence. LITERATURE CITED Dickson, R. C., E. F. Laird, Jr., and G. R. Pesho. 1955. The spotted alfalfa aphid (yellow clover aphid on alfalfa). Hilgardia 24: 93- 1 1 8. Ewert, M. A. and H. C. Chiang. 1966a. Dispersal of three species of coccinellids in corn fields. Can. Entomol. 98: 999-1003. Ewert, M. A. and H. C. Chiang. 1 966b. Effects of some environmental factors on the distribu- tion of three species of Coccinellidae in their microhabitat. pp. 195-219. In: I. Hodek [ed.]. Ecology of Aphidophagous Insects. Dr. W. Junk, Publishers, The Hague. 360 pp. Vol. 109, No. 4, September & October, 1998 281 Forbes, S. A. 1883. The food relations of the Carabidae and Coccinellidae. Bull. 111. State Lab. Nat. Hist. 1: 33-64. Gordon, R. D. 1985. The Coccinellidae (Coleoptera) of America north of Mexico. J. N.Y. Entomol. Soc. 93: 1-912. Hagen, K. S. 1962. Biology and ecology of predaceous Coccinellidae. Ann. Rev. Entomol. 7: 289-326. Hardy, A.V., C. B. Carney, and H. V. Marshall, Jr. 1967. Climate of North Carolina research stations. NC Agric. Exp. Stn. Bull. 443, 75 pp. Hilbeck, A. and G.G. Kennedy. 1996. Predators feeding on the Colorado potato beetle in insec- ticide-free plots and insecticide treated commercial potato fields in eastern North Carolina. Biol. Control 6: 273-292. Hodek, I. 1973. Biology of Coccinellidae. Dr. W. Junk Publishers, The Hague. 260 pp. Hodek, I. and A. Honek. 1996. Ecology of Coccinellidae. Kluwer Academic Publishers, Dordrecht. 464 pp. Hodek, I., G. Iperti, and M. Hodkova. 1993. Long distance flights in Coccinellidae (Co- leoptera). Eur. J. Entomol. 90: 403-414. Kidd, K. A. 1996. Coccinellids in wheat, 1996. Annual Report of Activities, Beneficial Insects Laboratory, NC Dep. Agric., Raleigh, p. 17. Klausnitzer, B. 1989. Marienkaferansammlungen am Ostseestrand. Entomol. Nachr. Ber. 33: 189-194. Klausnitzer, B. 1992. Coccinelliden als Pradatoren der Holunderblattlaus (Aphis sambuci L.) im Warmefruhjahr 1992. Entomol. Nachr. Ber. 36: 185-190. Lee, R. E., Jr. 1980. Aggregation of lady beetles on the shores of lakes (Coleoptera: Coccinellidae). Am. Midi. Nat. 104: 295-304. Majerus, M. E. N and T. M. O. Majerus. 1 996. Ladybird population explosions. Br. J. Entomol. Nat. Hist. 9: 65-76. Marriner, T. F. 1939. Movements of Coccinellidae. Entomol. Rec. 51: 104-106. Meadows, B. C. 1996. North Carolina Agricultural Statistics, 1996. NC Dept. Agric., Raleigh, 132pp. Oliver, F. W. 1943. A swarm of ladybirds (Coleoptera) on the Libyan desert coast of Egypt between Hammam and Abusir. Proc. R. Ent. Soc. Lond. (A) 18:87-88. Rothschild, M. 1971. A large migration of the seven-spot ladybird (Coccinella septempunctata L.) at Deauville, France. Entomologist (London) 104: 45-46. Schaefer, P. W., R. J. Dysart and H. B. Specht. 1 987. North American distribution of Coccinella septempunctata (Coleoptera: Coccinellidae) and its mass appearance in coastal Delaware. Environ. Entomol. 16: 368-373. Yan, J. J., Y. C. Chang and X. M. Cat. 1983. Observations on the aggregation of Coccinella septempunctata L. (Col.: Coccinellidae) in different coastal areas. Natural Enemies of In- sects (KunchongTiandi) 5: 100-103 [Rev. Appl. Entomol. Ser. A. 72: 2771]. PLEASE SEE ANNOUNCEMENT ON PAGE 276 282 ENTOMOLOGICAL NEWS BIOLOGICAL AND MORPHOLOGICAL NOTES ON DASYHELEA PSEUDOINCISURATA (DIPTERA: CERATOPOGONIDAE)1 Lawrence J. Hribar^ ABSTRACT: Dasyhelea pseudoincisurata larvae were collected from a waste tire and a bucket. Larvae presumably fed in algae in these containers, but after collection they fed only on dead insects. Larval thoracic pigmentation is described. Pupae move rapidly across the substrate with the aid of abdominal spicules. The mean pupal period was 2.1 days. Attempts to recover eggs from reared adults were unsuccessful. Little is known about the immature stages of many biting midges (Cerato- pogonidae), and even basic details of life history, including larval habitat and feeding behavior, are poorly understood for most species. During a survey of containers for Aedes albopictus (Skuse), a number of biting midge larvae were collected from an old tire and from a plastic bucket. These larvae were placed into a plastic petri dish along with water from the larval habitat. Pupae were collected and placed into separate dishes to await adult emergence. The adults reared from these larvae were identified as Dasyhelea pseudoincisurata Waugh &Wirth(1976). Dasyhelea spp. larvae may be found in a number of different habitats, in- cluding natural cavities. Dasyhelea pseudoincisurata has been collected from treeholes and cavities in rocks (Waugh & Wirth 1 976). Wirth & Waugh ( 1 976) found other Dasyhelea spp. larvae in cavities in tree stumps. Collections of Dasyhelea larvae from artificial containers are not often reported, but they are by no means unknown. For example, Remmert (1953) described Dasyhelea tecticola from specimens collected in rain gutters. When D. pseudoincisurata larvae were collected, their alimentary tracts were filled with a green material. This material was believed to be algae, however, microscopic examination of one larva's alimentary tract revealed mostly unidentifiable debris, although fungal spores were present. During rearing, larvae were fed dead mosquito, chironomid, and psychodid larvae, mosquito pupae, and a gravid adult female bibionid. These were first killed by crushing them with forceps, after which all were consumed by the Dasyhelea larvae. The Dasyhelea larvae began feeding at the wound site, and one Dasyhelea larva pulled a mosquito's alimentary tract outside of its body and began feeding on its contents. Other Dasyhelea larvae ignored the prey's ali- mentary canal and instead fed on fat body and muscle tissue of the mosquito. The feeding behavior of the Dasyhelea larvae resembled that of some Beizia 1 Received September 12, 1997. Accepted October 29, 1997. 2 Indian River Mosquito Control District, 5655 41st. Street, Vero Beach, FL 32967. Present ad- dress: Monroe County Mosquito Control District, 506 106th St., Gulf, Marathon, FL 33050. ENT. NEWS 109(4) 282-284, September & October, 1998 Vol. 109, No. 4, September & October, 1998 283 larvae (Hribar & Mullen 1 99 1 ). Some Dasyhelea larvae used their anal crochets to hold themselves in place while feeding. It appeared that only the third and fourth instar Dasyhelea larvae fed on mosquitoes. Twice, younger larvae were seen attempting to feed, but these were pushed aside by the vigorous feeding of the older larvae. Younger larvae are probably not strong enough to compete against older larvae. Dasyhelea larvae most often are said to feed on algae and fungi (Mullen & Hribar 1988). However, Lee & Chan (1985) reported that Dasyhelea ampullariae Macfie larvae fed on mosquito larvae in the same habitat. Mosquitoes and Dasyhelea grisea (Coquillett) can coexist in the same habitat with no apparent harm to either species (Baumgartner 1986). Thoracic pigmentation of the larvae was a brown coloration present on the dorsal aspect which extended toward the ventral surface on the prothoracic and mesothoracic segments. On these segments the pigment was distributed in an easily recognizable pattern (Fig. 1 ), but the prothorax was more strongly Ms Mt Fig. 1. Thoracic pigmentation of Dasyhelea larvae collected in waste tire; P - prothorax, Ms - mesothorax, Mt - metathorax. 284 ENTOMOLOGICAL NEWS pigmented than were other thoracic segments, with less pigmentation present on the abdominal segments. The crochets on the anal segment were large and arranged in a starburst pattern. The larva of D. pseudoincisurata is undescribed, and these characters may permit larvae to be distinguished from those of other species. Pupae were placed into separate plastic petri dishes lined with moist filter paper at ambient room temperature, which varied from 23° C to 26° C (74° F to 78° F). Pupae were active and used their large distinctive spicules to move rapidly across filter paper. Average length of the pupal stage was 2.1 days; 23 adults were reared. Adult males used their foretarsi to groom their antennae. There are long spines at the base of the tarsus, and perhaps these also are used for grooming. Adults were provided with 10% sucrose solution as an energy source for possible mating and oviposition, but these attempts to collect eggs were not successful. While laboratory conditions do not duplicate natural conditions, D. pseudoincisurata larvae will feed on a range of offered prey. The distinctive pattern of the larvae may be useful for species identification. I thank W.L. Grogan, Jr., Salisbury State University, for identifying the midges. D.A. Shroyer, Indian River Mosquito Control District, and G.L. Miller, USDA, com- mented on the manuscript LITERATURE CITED Baumgartner, D.L. 1986. Failure of mosquitoes to colonize teasel axils in Illinois. J. Amer. Mosq. Control. Assoc. 2: 371-373. Hribar, L.J. & G.R. Mullen. 1991. Predation by Bezzia larvae (Diptera: Ceratopogonidae) on mosquito larvae (Diptera: Culicidae). Entomol. News. 102: 183-186. Lee, K.M. & K.L. Chan. 1985. The biology of Dasyhe lea ampullariae in monkey cups at Kent Ridge (Diptera: Ceratopogonidae). J. Singapore Nat. Acad. Sci. 14: 6-14. Mullen, G.R. & L.J. Hribar. 1988. Biology and feeding behavior of ceratopogonid larvae (Diptera: Ceratopogonidae) in North America. Bull. Soc. Vector Ecol. 13: 60-81. Remmert, H. 1953. Dasyhelea tecticola n.sp., eine Ceratopogonidae aus Regenrinnen (Diptera: Ceratopogonidae). Beitr. z. Entomol. 3: 333-336. Waugh, W.T. & W.W. Wirth. 1976. A revision of the genus Dasyhelea Kieffer of the eastern United States north of Florida. Ann. Entomol. Soc. Amer. 69: 219-247. Wirth, W.W. & W.T. Waugh. 1976. Five new Neotropical Dasyhelea midges (Diptera: Ceratopogonidae) associated with culture of cocoa. Studia Entomol. 19: 223-236. PLEASE SEE ANNOUNCEMENT ON PAGE 276 Vol. 109, No. 4, September & October, 1998 285 EASTWARD RANGE EXTENSION IN CANADA OF THE ALDERFLY SIALIS VELATA (MEGALOPTERA: SIALIDAE), AND THE POTENTIAL OF THE GENUS AS A CONTAMINANT MONITOR1 Isabelle Roy2, Landis Hare^ ABSTRACT: We report on a range extension for Sialis velata (Megaloptera: Sialidae) in eastern Canada and assess the potential of the genus as a metal biomonitor. Sialis velata Ross is reported from the province of Quebec for the first time since its original collections (Ross 1937). Ours is the first record of any Sialis species from the province since 1937. All previous collections of the five Sialis species known from the province (S. velata, S. iola, S. itasca, S. mohri and S. vagans) were from the extreme southwestern corner near the city of Montreal (» 44°N, 73°W) (Ross 1937; Whiting 1991). It is now possible to extend the range of 5. velata, north to the 47th parallel, and east to the Quebec City region (71°W). Ours is the most easterly record for S. velata in Canada. Only two Sialis species are known to occur in Canada east of Quebec City, i.e., 5. mohri (New Brunswick) and S. vagans (New Brunswick and Nova Scotia) (Ross 1 937, Whiting 1 99 1 ). Because we have found Sialis larvae in the major- ity of the Quebec lakes that we have visited, the lack of collection records in eastern Canada is likely a consequence of a lack of effort in collecting adults and rearing larvae rather than the rarity of the genus in eastern Canada. Adults of S. velata (five males, three females) were reared from a large number of larvae collected on May 1 8 1 997, from soft mud at a depth of 5 m in a Shield lake (Lake St- Joseph; 46°55'N, 71°40'W). Sialis larvae for rearing were held individually in small plastic containers, in 50 ml of water that was renewed weekly, and fed live chironomid larvae. Gut content analysis indi- cated that larvae in the field fed largely on chironomids and oligochaetes, as has been reported by previous investigators (Azam and Anderson 1969, Pritchard and Leischner 1973, Canterbury 1978). When individual larvae ex- hibited agitated swimming behavior at the water surface they were transferred to a small plastic container filled with a mixture of sand and soil for pupation. In the laboratory, pupation and adult emergence were highly successful at room temperature (10% mortality), but not at 10°C (100% mortality). Our results are consistent with those of Elliott (1996), who found that pupation success could be described by a quadratic equation with a threshold at approximately C and an optimum at 15°. 7° 1 Received October 20, 1997. Accepted January 21, 1988. 2 INRS-Eau, Universite du Quebec, C./P. 7500, Sainte-Foy, Quebec, Canada GIV 4C7. 3 Author to whom correspondence should be addressed. ENT. NEWS 109(4) 285-287, September & October, 1998 286 ENTOMOLOGICAL NEWS Our interest in the larvae of Sialis relates to their potential as a contami- nant biomonitor by virtue of the following properties (with quotations from Phillips and Rainbow 1993): 1 . Widespread and abundant «Biomonitors should be abundant throughout the study area.» The genus occurs throughout the Holarctic region. In our studies, larvae were collected in 2 1 of 34 Quebec and Ontario lakes sampled in a cursory manner. This ease of collection suggests that larvae are abun- dant when present. 2. Easily kept in the laboratory «The organism used should be easy to sample and hardy enough to survive under laboratory conditions. » We have cap- tured Sialis larvae using both grabs and diver-operated benthic nets. We can keep Sialis larvae for periods of up to 3 months at 10°C in the labora- tory in water alone (no sediment) with weekly feeding of live chironomid larvae. Given this ease of maintenance in the laboratory, Sialis larvae could be used to probe mechanisms of contaminant accumulation and toxicity. 3. Large size«The organism should provide sufficient tissue for contami- nant analysis. » We have successfully measured several trace metals in whole individual larvae as well as in various larval tissues (pooled samples of several individuals; Hare et al. 1991). 4. Contaminant tolerant «Contaminants should be accumulated without le- thal impacts to the species employed. » An effective biomonitor should be found along the full range of contaminant concentrations encountered in nature. Sialis species are found in lakes influenced by acid mine drainage (e.g., Gatewood and Tarter 1983), or metal smelters (Hare and Tessier, un- published), both of which tend to be characterized by low pH's (down to pH 4) and high concentrations of trace metals (e.g., up to 14 nM total dis- solved Cd). This is a clear indication of metal and hydrogen ion resistance in Sialis. 5. Relation between animal and contaminant concentrations «A simple correlation should exist between the contaminant content of a biomonitor and the average contaminant concentration in its ambient environment. » Initial analysis of data from 17 lakes suggests that Cd concentrations in Sialis are directly related to those of the free metal ion, Cd2+, when the competitive effect of hydrogen ions on biological uptake sites is taken into account (e.g., Hare and Tessier 1996). Given the above-described characteristics of Sialis larvae, their potential as a contaminant biomonitor seems high. However, the effective use of these Vol. 109, No. 4, September & October, 1998 287 larvae as metal biomonitors could be improved by acquiring more information on their: (i) Feeding: we do not know how Sialis larvae obtain trace metals, i.e. from the water in contact with their gills, or from the food they eat. This infor- mation will help us to determine if food-related variables such as prey type, trophic position and metal assimilation rates (at present largely unknown) should be included in the development of predictive bioaccumulation models; (ii) Burrowing: because Sialis larvae are burrowers (Charbonneau et al. 1997), more information on the depth, form and rate of burrowing could be useful in understanding their exposure to sedimentary metals. ACKNOWLEDGMENTS We thank Michael F. Whiting for the identification of S. velata. For their technical assis- tance, we thank M.-R. Doyon, J.P. Baillargeon, B. Patry, and R. Rodrigue. Funding is acknowl- edged from Fonds pour la formation de Chercheurs et 1'aide a la Recherche (F.C.A.R.) and Hu- man Resources Development Canada. LITERATURE CITED Azam, K.M. and N.H. Anderson. 1969. Life history and habits of Sialis rotunda and S. californica in Western Oregon. Ann. Ent. Soc. Am. 62:549-558. Canterbury, L.E. 1978. Studies on the genus Sialis (Sialidae :Megaloptera) in Eastern North America. Unpubl. Ph. D. thesis, Univ. of Louisville, Louisville, KY. Charbonneau, P., L. Hare and R. Carignan. 1997. Use of X-ray images and a contrasting agent to study the behavior of animals in soft sediments. Limnol. Oceanogr. 42: 1823-1828. Elliott, J.M. 1996. Temperature-related fluctuations in the timing of emergence and pupation of Windermere alder-flies over 30 years. Ecol. Ent. 21:241-247. Gatewood, R.W. and D.C. Tarter. 1983. Life history and ecology of the alderfly, Sialis aequalis Banks, from Flatfoot Creek, Mason County, West Virginia. Proc. W. Va. Acad. Sci. 2: 102- 113. Hare, L., A. Tessier and P.G.C. Campbell. 1 991 . Trace element distributions in aquatic insects: variations among genera, elements and lakes. Can. J. Fish. Aquat. Sci. 48: 1481-1491. Hare, L. and A. Tessier. 1996. Predicting animal cadmium concentrations in lakes. Nature 380:430-432. Phillips, DJ.H. and P.S. Rainbow. 1993. Biomonitoring of trace aquatic contaminants. Elsevier Applied Science, NY. Pritchard, G. and T.G. Leischner. 1973. The life history and feeding habits of Sialis cornuta Ross in a series of abandoned beaver ponds (Insecta;Megaloptera). Can. J. Zool. 51:121-131. Ross, H.H. 1937. Studies of Nearctic aquatic insects I. Nearctic alderflies of the genus Sialis (Megaloptera: Sialidae), Bull. 111. Nat. History Surv. 21(3):57-78. Whiting, M.F. 1991. A distributional study of Sialis (Megaloptera : Sialidae) in North America. Ent. News 102:50-57. 288 ENTOMOLOGICAL NEWS GYNANDROMORPH OF HELICOVERPA ARMIGERA [LEPIDOPTERA : NOCTUIDAE]1 A. Josephrajkumar, B. Subrahmanyam, V.V. Ramamurt h \ ~ ABSTRACT: A gynandromorphic moth was observed in a laboratory culture of the American bollworm, Helicoverpa armigera, with external characters of female on the left and of male on the right side. Dissection revealed the presence of a testis and an ovary on the trans-lateral posi- tions of the moth, respectively. A gynandromorph is an individual in which one part of the body is mascu- line and the other is feminine. Among insects, bilateral gynandromorphs are most frequent, in which the left and right halves are of different sexes (Mayr and Ashlock, 1991). However, anteroposterior gynandromorphs and forms with irregular mosaic-like distributions of sexual characters also are known (Richards andDavies, 1977). In Drosophila melanogaster of XX chromosomal constitution, a gynan- dromorph arises through the loss of one X chromosome in one of the early cleavage nuclei of the embryo, so that deficient (XO) cells form male tissue while those with a full complement of sex chromosomes yield female tissues (Wilbert, 1953). Gynandromorph also can result from the "double fertiliza- tion" of abnormal eggs possessing two nuclei, one of which gives rise to male and the other to female tissues (White, 1968). Some parasitic Hymenoptera yield many gynandromorphs at unusually high temperatures (Bowen and Stern, 1966). Lepidopteran gynandromorphs have been recognised in species with sexual dimorphism where the male has coloration and/or pattern elements that typi- cally differ from those of the female. Hence, notable sexual differences in phenotype appear on the two halves of the adult. An extraordinary hybrid gy- nandromorph containing wing-pattern genes from at least three subspecies of Heliconius melpomene (Lepidoptera : Nymphalidae) was reported by Emmel and Boender (1990). A rare gynandromorph of Nacophora quernaria (Lepi- doptera : Geometridae) from Florida showed a perfectly bilateral division be- tween the male and female with different antennae, the thorax, and the poste- rior anal tufts of the abdomen (Kutis and Heppner, 1990). At the Division of Entomology, Indian Agricultural Research Institute, New Delhi, a gynandromorph of Helicoverpa armigera arose for the first time in a laboratory colony reared on the artificial diet of Singh and Rembold (1992). Incidentally, this has been observed in a routine experiment with plumbagin, a napthaquinone of plant origin having insect growth regulatory activity, where the larva received a dose of 100p,g g-' applied topically. 1 Received August 25, (1997). Accepted December 17, 1997. 2 Division of Entomology, Indian Agricultural Research Institute, New Delhi - 1 1 0 0 1 2 India. ENT. NEWS 109(4) 288-292, September & October, 1998 Vol. 109, No. 4, September & October, 1998 289 Over 20 generations, the normal coloration of the moths in the laboratory culture is unaffected by the diet and they perfectly resembled those collected at light or those emerged from larvae collected from chickpea, pigeonpea and cotton fields. The female moth of//, armigera is dull orange-brown with a ' V shaped marking on forewing and dull black border on the hindwing. Forewing of male is greyish-green and poorly marked beyond the transverse posterior line (Hardwick, 1965). The single gynandromorph specimen observed had an orange-brown col- ored forewing on the left side and greyish-green colored forewing on the right. The color pattern fits perfectly with that of a female on the left and male on the right. The posterior anal tufts of the abdomen were prominent on both sides [Fig. 1 ]. Dissection of the moth revealed the presence of a testis and accessory glands on the left side and an ovary and colleterial glands on the right side [Fig. 2]. The placement of sex organs is therefore opposite to that of the sexual differences in the external color pattern. The specimen is unique in this regard. Though the moth survived for over one week on 10% honey solution, it is not known if the moth might have been reproductively viable. Examination of the moths of this species from the National Pusa Collection of our Division has not revealed the occurrence of any specimen with such a mosaic of external coloration. Fig. 1 Gynandromorph of Helicove rpa armigera (Hubner) Left side forewing : female pattern, right side : male pattern ( Scale line = 0.5 cm ). 290 ENTOMOLOGICAL NEWS Fig. 2 Reproductive organs of the gynandromorph ( Note the testis (T) on the left side and the ovary (O) on the right side, the opposite placement of the wing pattern) (Scale line = 2.0 mm). DISCUSSION Females of Lepidoptera are heterogametic (ZW) whereas males are ho- mogametic (ZZ). The chromosomal constitution of eggs, rather than the sperm cells, determine the sex of the progeny. Gynandromorphs may arise due to loss of the Z chromosome during early zygotic divisions or due to double fertiliza- tion of binucleate (ZW) eggs. The loss of a Z chromosome in ZZ zygotes is a major cause of gynandromorphism (Robinson, 1971). This results in an em- bryo that is both female (ZO) and male (ZZ). The gynandromorph noticed in this study is an interesting case and deserves careful analysis. We found no reference to such a condition in the literature on gynandromorphs. Mitotic spindle orientation at the first zygotic division is always random. In D. melanogaster subsequent mitotic products of the first two daughter nu- clei do not intermingle before they migrate to the cellular blastoderm. Conse- quently, a half male/female gynandromorph shows large contiguous patches of male/female tissue, whose pattern of distribution is governed by the orien- tation of the first zygotic spindle. A left versus right symmetrical distribution of male versus female cuticular tissue in this H. armigera gynandromorph Vol. 109, No. 4, September & October, 1998 291 specimen demonstrates that early blastoderm cells in H. armigera do not inter- mingle as in D. melanogaster. Gynandromorphs are powerful tools for following the clonal history of cells and producing fate maps of the blastoderm (Hotta and Benzer, 1972). The adult wings are derived from wing imaginal discs while the male and female somatic reproductive structures are mesodermal in origin. Ferrus and Kankel (1981), in a mosaic analysis of cuticular and muscular tissues in D. melanogaster, showed that wing epithelial cells often attach to clonally unre- lated muscle cells. Even though the converse was not stated by Ferrus and Kankel (1981), it implies that clonally unrelated cells come to occupy extrin- sically-related positions due to extensive cell movement in Drosophila during embryogenesis. Comparatively little is known about embryogenesis and mor- phogenetic movements in Helicoverpa. Considering that male and female re- productive cells of mesodermal origin occupy positions trans-lateral to clonally- related epidermal cells in our//, armigera gynandromorph specimen, we con- clude that similar extensive cell movements occur in H. armigera during me- sodermal differentiation also. The influence of pesticidal molecules, including plumbagin, on epistatic sex determination has not been well-studied. Intersexes arise due to distur- bances of the epistatic relationship between male and female determining genes during development. Such forms are common in Aedes sp. exposed to higher temperatures and in Lymantria dispar when genetically distinct strains are crossed (Richards and Davies, 1977). Therefore, the insect under study can at the best be regarded as a natural gynandromorph occurring at an extremely low frequency (0.000125%, i.e., one out of 8000 insects reared so far), as also observed in N. quernaria by Kutis and Heppner ( 1 990) and in //. melpomene by Emmel and Boender ( 1 990). ACKNOWLEDGMENTS The authors are extremely grateful to N. Ramakrishnan, Division of Entomology and Shanti Chandrasekharan, Division of Genetics, I.A.R.I., New Delhi for reviewing the manuscript. LITERATURE CITED Bowen, W. R. and V. M. Stern. 1966. Effect of temperature on the production of males and sexual mosaics in an uniparental race of Trichogramma semifumatum (Hymenoptera : Trichogrammatidae). Ann. Entomol. Soc. Am. 59: 823 - 834. Emmel, T. C. and R. Boender. 1990. An extraordinary hybrid gynandromorph of He liconius melpomene subspecies (Lepidoptera : Nymphalidae). Trop. Lepid. 1: 33 - 34. Ferrus, A. and D.R. Kankel. 1981. Cell lineage restrictions in Drosophila melanogaster. The relationship of cuticular to internal tissue. Dev. Biol. 85 : 485 - 504. Hardwick, D.F. 1965. The corn earworm complex. Memoirs of the Entomological Society of Canada. No.40. 246pp. Hotta, Y. and S. Benzer. 1972 . Mapping of behaviour in Drosophila mosaics. Nature 240 : 527- 535. 292 ENTOMOLOGICAL NEWS Kutis, J. S. and J. B. Heppner. 1 990. Gynandromorph of Nacophora quernaria in Florida (Lepi- doptera : Geometridae). Trop. Lepid. 1: 42. Mayr, E. and P. Ashlock. 1991. Principles of Systematic Zoology. 2nd ed. McGraw Hill Inc. London. 475 pp. Richards, O. W. and R. G. Davies. 1977. Imms' General Textbook of Entomology. 10th ed. Vol.1, Chapman and Hall Ltd., London. 418 pp. Robinson, R. 1971. Lepidoptera Genetics. Pergamon press. Oxford 687 pp. Singh, A. K. and H. Rembold. 1992. Maintenance of the cotton bollworm Heliothis armigera (Hubner) (Lepidoptera : Noctuidae) in laboratory culture - I. Rearing on semisynthetic diet. Insect Sci. Applic. 13 : 333 - 338. White, M. J. D. 1968. A gynandromorphic grasshopper produced by double fertilization. Aust. J. Zool. 16: 101 - 109. Wilbert, H. 1953. Normales und experimental beeinflusstes Auftreten von Mannchen und Gynandromorphen der Stabheuschrecke. Zool. Jb. (Allg. Zool.) 64: 470 - 495. SOCIETY MEETING OF APRIL 22, 1998 Andrew Short Glasgow High School/Univesity of Delaware ENGINEERED STREAM SYSTEMS AS TOOLS FOR MACROINVERTEBRATE RESEARCH Mr. Short has done research on the effects of elevated nutrient levels on benthic macroinvertebrates. He has designed and used artificial stream systems that are capable of maintaining extended pristine conditions. He received the Society's Calvert Award in 1996 for his work. In the first part of his talk, Short discusssed the uses of artificial streams in dealing with riparian ecosystems as well as their applications in macroinvertebrate studies. Designs of his own stream systems were shown along with designs from Stroud Water Research Center and other engi- neered environments. Construction materials and methods for engineered environment construc- tion were presented. Procedures for the collection of sediments and organisms, such as commu- nity block injection, and system operation were also given. Short described the function and uses of algal turf scrubber technology as an advisable alternative to other water filters and purifiers. During the second part of this talk, Short presented his current research on the effects of elevated nutrient levels on benthic macroinvertebrates in artificial streams. Short outlined the procedures for data collection in the engineered systems. He discussed his results, in which certain popula- tions of macroinvertebrates, primarily Trichoptera: Hypopsychidae, experienced significant de- clines over control populations, immediately after nutrient elevation. The nutrient factors used in the initial trials were nitrate nitrogen and soluble phosphorus, in concentrations consistent with poultry manure runoff that might be experienced regionally as a consequence of current agricul- tural practices. These population declines took place without the effects of eutrophication taking place. In subsequent trials, nitrate nitrogen elevation alone showed no negative effect on similar macroinvertebrate populations. - W. J. Cromartie, Correspnding Secretary ENT. NEWS 109(4) 292, September & October, 1998 Vol. 109, No. 4, September & October, 1998 293 A NEW SPECIES OF SWELTSA (PLECOPTERA: CHLOROPERLIDAE) FROM EASTERN NORTH AMERICA1 Boris C. Kondratieff2, Ralph F. Kirchner3'4 ABSTRACT: Sweltsa holstonensis, new species, is described from southwestern Virginia. The new species is most similar to Sweltsa urticae. The male epiproct of both species are illustrated for comparison. The genus Sweltsa in the Eastern Nearctic is represented by eight species (Kondratieff and Kirchner 1991). These species, with the exception of S. naica (Provancher), form a monophyletic lineage based on male epiproct shape. Two species of this group, 5. urticae (Ricker) and S. voshelli Kondratieff and Kirchner, are related and constitute a distinctive clade within the group. Both species have the epiproct broadly dorsolaterally flanged and occur in small streams of the Southern Appalachian Mountains. A third member belonging to this clade was recently discovered in southwest Virginia by Bill P. Stark and Ralph F. Kirchner. Sweltsa holstonensis NEW SPECIES (Figs. 1-2) Male.- Body length 7.5-8.0 mm. Macropterous, length of forewing 8.5-9.0 mm. General color bright yellow in life, yellow-white in alcohol. Head with three dark ocellar rings. Pronotum with black margins, no median stripe. Middorsal region of abdominal terga 1-9 with black dashes or stripes. Terga 9 with transverse ridge (Figs. 1 -2). Epiproct erectile, inflated in dorsal view (Fig. 1 ), forming a flange laterally for most of its length, covered with appressed golden hairs; in lateral view deeply incurved on distal fourth forming a dorsally directed hook, apex projecting above dorsal plane and not transversely compressed; ventral aspect convex in lateral view (Fig. 2). Female.- Body length 9.5-10.5 mm. Macropterous, length of forewing 10-10.5 mm. General color and pattern as male. Subgenital plate about as long as wide, lateral margins slightly incurved; apex acutely rounded, about one-fifth width of plate. Types.- Holotype male, Washington County, Virginia, Little Moccasin Creek, County Rt. 690, 15 May 1997, B. P. Stark and R. F. Kirchner. Paratypes, same data as holotype, 2 males; same data as holotype but 19 May 1998, R. F. Kirchner, 3 males, 3 females. The holotype will be deposited in the United States Museum of Natural History, Smithsonian Institution, Washington D.C., and paratypes in the collection of R. F. Kirchner and the C.P. Gillette Museum of Arthro- pod Diversity, Colorado State University. 1 Received May 4, 1998. Accepted August 13, 1998. 2 Department of Bioagricultural Sciences and Pest Management, Colorado State University, Fort Collins, CO 80523. - Department of the Army, Huntington District Corps of Engineers, Water Quality Section, P.O. Box 9, Apple Grove, WV 25502. 4 The views of this author do not purport to reflect the position of the Department of the Army or the Department of Defense. ENT. NEWS 109(4) 293-295, September & October, 1998 294 ENTOMOLOGICAL NEWS Etymology.- The specific epithet refers to the Holston Valley of the Great Appalachian Valley of Virginia. In Virginia, the Great Valley is divided into the Shenandoah, Roanoke, New, and Holston Valleys based on drainage systems. Little Moccasin Creek flows into the North Fork of the Holston River at Holston. Diagnosis.- Males of S. holstonensis can be distinguished from S. urticae by the hook of the epiproct lacking the transversely compressed apex (Fig. 3), epiproct ventrally convex, hook of epiproct usually exceeding the level of the dorsal plane, and flange of epiproct covered with appressed golden hairs. Additionally, the male of S. holstonensis can be separated from the only other similar species, S. voshelli, by an epiproct only 2 to 3 times as long as the greatest width and not gradually tapering to the apex (Figs. 2 and 3, Kondratieff and Kirchner 1991). The female is similar to both S. urticae and S. voshelli, but can be distinguished from S. voshelli by the acutely rounded apex of the subgenital plate (S. voshelli, the apex is broadly rounded), and from S. urticae by the longer and narrower apex, about one-fifth width of subgenital plate. Remarks.- Little Moccasin Creek is a high gradient mountain stream originating at Low Gap (1 150.3 m) between the Clinch and Brumley Mountains. It flows Fig. 1 .-3. Sweltsa holstonensis. 1 . male terminalia, dorsal. 2. epiproct, lateral view. Sweltsa urticae. 3. epiproct, lateral view; inset, tip. Vol. 109, No. 4, September & October, 1998 295 south 8.04 km to its confluence with the North Fork of the Holston River (438.9 m) at Holston, just northwest of Abingdon. Most of the original forest was cleared for upland pasture. Virginia's threatened peltoperlid, Tallaperla lobata Stark, was also collected from Little Moccasin Creek near Low Gap (Kondratieff and Kirchner 1991). Other species collected with 5. holstonensis included Alloperla usa Ricker, 5. lateralis (Banks), S. onkos (Ricker), and Yugus n. sp. ACKNOWLEDGMENTS We thank Bill P. Stark, Mississippi College for making the material available. Lynn C. Bjork produced the illustrations. LITERATURE CITED Kondratieff, B. C. and R. F. Kirchner. 1991. New Nearctic Chloroperlidae (Plecoptera). J. N.Y. Entomol. Soc. 99: 199-203. Kondratieff, B. C. and R. F. Kirchner. 1991. Stoneflies. Pp. 214-225. In: Virginia's Endan- gered Species. K. Terwilliger (ed.). McDonald & Woodward Publ. Co., Blacksburg, VA. BOOKS RECEIVED AND BRIEFLY NOTED RECENT ADVANCES IN ARTHROPOD ENDOCRINOLOGY. G.M. Coast & S.G.Webster, eds. 1998. Cambridge Univ. Press. 406 pp. $1 10.00 (hardcover). Nearly fifty contributors describe current work in selected areas of arthropod endocrinology and highlight directions future studies may take. Endocrine mechanisms are dealt with in the first sixteen chapters, while the final two chapters are concerned with peptide processing and the development of stable lipophilic peptidomimetics. THE BUTTERFLIES OF WEST VIRGINIA AND THEIR CATERPILLARS. Thomas J. Allen. 1997. Univ. of Pittsburgh Press. 388 pp. 50 plates in color. $37.50 (cloth); $22.95 (trade paper). Descriptions of 1 28 species of butterflies, along with their caterpillars and pupae, found in West Virginia. Each species account provides a description and information on distribution, habitat, life history, nectar sources, and larval host plants. Twenty of the fifty colored plates depict larvae and pupae, many not published elsewhere. Included are chapters on studying butterflies and butterfly gardening. INSECT HORMONES. H. E. Frederick Nijhout. A 1998 paperback edition of a 1994 edition. Princeton Univ. Press. 267 pp. ($19.95 (paper). The emphasis in this book on insect endocrinology is on the biology of the organism and the ways in which physiological and developmental regulatory mechanisms are integrated into the insect's life cycle. 296 ENTOMOLOGICAL NEWS SOCIETY MEETING OF MARCH 25, 1998 Dr. Joseph K. Sheldon and Mr. Jeff Erikson Department of Natural Science, Messiah College SERPENTINE BARRENS OF PENNSYLVANIA AND MARYLAND Dr. Sheldon began by explaining the nature of serpentine barrens, where they are located, and why they are important ecologically. It was pointed out that the unique soil conditions have resulted in an unusual plant community. The goal of the research currently being conducted by Dr. Sheldon and Mr. Erikson is to determine whether an equally unusual community of msects is associated with the unique plant community in the Nottingham and Goat Hill Serpentine Bar- rens. Dr. Sheldon briefly described the survey work already completed on the Lepidoptera by The Nature Conservancy: five species of butterflies and 1 1 species of moths from Nottingham are listed as rare, threatened, or endangered. Seven endangered Lepidoptera have been recorded from Goat Hill. Dr. Sheldon's work on the Acrididae has found 18 species within the two bar- rens. Grasshoppers are seasonally active within the barrens from April until the first killing frosts of October or November. No species of special concern (as monitored by The Nature Conser- vancy) have been encountered to date in the study. It was pointed out that this is not surprising since our current knowledge of Acrididae is not sufficient for most species to be evaluated in terms of their rarity. Additional natural history work on most species across their ranges will be required. The purpose of Mr. Erikson 's study was to gather baseline macroinvertebrate community data and to determine if a proposed trout hatchery could utilize Black Run's water. Black Run is a three mile long tributary which flows over serpentine geology in Nottingham Barrens on its way to Ocotaro Creek (a tributary to the Susquehanna River). Jeff reported on two unique chemical characteristics at Black Run: a low calcium hardness and a high pH. These parameters did not negatively affect the macroinvertebrate community richness. Over the past nine months he collected more than 100 genera of macroinvertebrates among four sites along this stream. He outlined the different macroinvertebrate orders found and showed their spatial and temporal distributions. Jeff reported a phoretic relationship between Chironomidae: Plecopteracoluthus and Corydalidae: Anchytarsus at Black Run. He concluded that Black Run has a diverse macroinvertebrate community, which could be utilized by trout as food, but he has yet to determine water temperatures and pH levels during the summer season to see if these parameters are too high for trout survival. In notes of entomological interest. Dale Schweitzer reported that the past winter moth sea- son was the worst ever in southern New Jersey, but that butterflies were present unusually early: two or three species in January, as many as five species in an hour of searching in February. Included were numerous male Celastrina "Azures." Butterfly numbers went down in March, following a cold spell. - W. J. Cromartie, Correspnding Secretary ENT. NEWS 109(4) 296, September & October, 1998 When submitting papers, all authors are requested to (1) provide the names of two qualified individuals who have critically reviewed the manuscript before it is submitted and (2) suggest the names and addresses of two qualified authorities in the subject field to whom the manuscript may be referred by the editor for final review. All papers are submitted to recognized authorities for final review before acceptance. Titles should be carefully composed to reflect the true contents of the article, and be kept as brief as possible. Classification as to order and family should be included in the title, except where not pertinent. Following the title there should be a short informative abstract (not a descriptive abstract) of not over 150 words. 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The following books are available from Kendall/Hunt Publishing: An Introduction to the Aquatic Insects of North America edited by Richard W. Merrill and Kennelh W. Cummins (1995/880 pages/wire coil7$69.95*/lSBN 0-7872-3241-6 or 1995/880 pages/olabind/S78.69*/ ISBN 0-7872-3240-8). Aquatic Insects, wilh readings wrilten by 41 experts, will quickly become your slandard reference book, ll includes features such as: com- prehensive coverage of behavior, collecting, biomoniloring, and taxonomy; well-illuslraled keys lo major life stages of Ncrth American aquatic insects; and tables at the end of every identification chapter with summaries at the generic level of the ecology, habits, and distribu- tion of the order or family of aquatic insects. Immature Insects, Volumes I and II, edited by Frederick W. Stehr (Volume I: 1987/768 pages/ casebound/$136.44*/ISBN 0-84034639-5 and Volume II: 1991/992 pages/casebound/ 241.44*/ISBN 0-8403-4639-5). Immature Insects provides information on the biology and ecology of the families and selected important species. The two volume set also gives you a means to identify insects ranging from the most common to the extremely rare through use of the abundant illustrations, descriptions, and/or keys to selected species. Immature Insects is the only reference that extensively covers updated information necessary to identify imma- ture insects. These books also describe techniques necessary for the collecting, rearing, kill- ing, preserving, storing, and studying of insects. Both books also include an introduction defining how terms are used in the book, a complete glossary, and an extensive index. For more information or to place an order, call Jill Crow at 1-800-228-0564. *A11 prices are subject to change. FOR SALE: Quality insect pins, black enamelled, stainless steel. Best prices guaranteed. Call for free samples. Phone: 1(800) 484-7347 Ext. 1324. Fax: (352) 37 1-69 18. E-mail: morpho@afn.org or write to Morpho Ventures, P.O.BOX 12454, Gainesville, Florida 32604. FOR SALE: Baltic amber pieces with insects, spiders, plants. Material for scientific work, as well as better pieces for display and teaching. O. Holden, Junkerg. 37, S-126 53 Hegersten, Sweden, fax: 01146-8-7268522. FOR SALE: Light traps, 12 volt DC or 1 10 volt AC with 15 watt or 20 watt black lights. Traps are portable and easy to use. Rain drains and beetle screens protect specimens from damage. For a free brochure and price list, contact Leroy C. Koehn, 207 Quail Trail, Greenwood, MS 38930-7315. Telephone 601-1-455-5498. VOL. !()«) NOVI;MBI;K & m.c I:MIU:K, I'S ISSN 0013-X72X NO. 5 ENTOMOLOGICAL NEWS Cerococcus michaeli (Homoptera: Cerococcidae): a new species of false pit scale from New Zealand Paris Lambdin 291 Larva of Macrothemis inacuta (Odonata: Libellulidae) R. Novelo-Gutierrez, A. Ramirez 301 A new species of Neotropical genus Bythonia (Homoptera: Cicadellidae) and the female ofB.consensa M. Felix, G. Mejdalani 307 Perlesta golconda (Plecoptera: Perlidae), a new stonefly species from Illinois R.E. DeWalt, B.P. Stark, MA. Harris 315 Two new species ofChorebus (Hymenoptera: Braconidae) from Spain /. Docavo, J. Tormos 318 Dipterous parasitoids from adults of moths (Lepidoptera) Timothy L. McCabe 325 Biology of Tanychela pilosa (Hymenoptera: Ichneumon- idae), a parasitoid of aquatic moth Petrophila confusalis (Lepidoptera: Pyralidae) W. Jamie son, V.H. Resh 329 New Ohio records of Corixidae (Hemiptera) S.W. Chordas, III, B.J. Armitage 339 Two corrections: Aradidae, Tingidae (Heteroptera) Richard C. Froeschner 343 New name for a generic homonym in Teloga- nodidae (Ephemeroptera) W.P. McCafferty, T.-Q. Wang 344 A new North American genus of Baetidae (Ephemeroptera) and key to Baetis complex genera C.R. Lugo-Ortiz, W.P. McCafferty 345 A new species of the small minnow mayfly genus Plauditus (Ephemeroptera: Baetidae) from South Carolina W.P. McCafferty, R.D. Waltz 354 New species of Cloeon and Demoulinia (Ephemeroptera: Baetidae) from Madagascar C.R. Lugo-Ortiz, W.P. McCafferty 357 Aedes albopictus (Diptera: Culicidae) occurrence throughout Tennessee, with biological notes James P. Moore 363 Distribution and habitat ofCurictapronotata (Hemiptera: Nepidae) in southeastern Arizona J.D. Hockstra, R.L. Smith 366 Hexamethyldisilazane, a chemical alternative for drying insects BOOKS RECEIVED & BRIEFLY PUBLISHER'S STATEMENT MAILING DATES - VOLUME 1 INDEX - VOLUME 109 aty , D. Hawks 369 314, 324 375 375 376 TIIK AMERICAN ENTOMOLOGICAL SOCIETY ENTOMOLOGICAL NEWS is published bi-monthly except July-August by The American Entomological Society at the Academy of Natural Sciences, 1900 Benjamin Franklin Parkway, Philadelphia, Pa., 191 03-1195, U.SA. The American Entomological Society holds regular membership meetings on the fourth Wednesday in October, November, February, March, and April. The November, February and April meetings are held at the Academy of Natural Sciences in Philadelphia, Pa. The October and March meetings are held at the Department of Entomology, University of Delaware, Newark, Delaware. Society Members who reside outside the local eastern Pennsylvania, southern New Jersey, and Delaware area are urged to attend society meetings whenever they may be in the vicinity. Guests always are cordially invited and welcomed. Officers for 1997-1998: President: Jon K. Gelhaus; Vice-President: Susan P. Whitney; Recording Secretary: Jeffrey S. Erikson; Corresponding Secretary: William J. Cromartie; Treasurer: Howard P. Boyd. Publications and Editorial Committee: Howard P. Boyd, Chr., D. Otte, and Norman E. Woodley. Previous editors: 1890-1920 Henry Skinner (1861-1926); 1921-1944 Philip P. Calvert (1871- 1961); 1945-1967 R.G. 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Boyd, 232 Oak Shade Road, Tabernacle Twp., Vincentown, New Jersey 08088, U.S.A. Manuscripts will be considered from any authors, but papers from members of the American Entomological Society are given priority. It is suggested that all prospective authors join the society. All manuscripts should follow the format recom- mended in Scientific Style and Format: The CBE Manual for Authors, Editors, and Publish- ers, Sixth Edition, and should follow the style used in recent issues of ENTOMOLOGICAL NEWS. Three doublespaced, typed copies of each manuscript are needed on 8'/2 x 1 1 paper. The receipt of all papers will be acknowledged and, if accepted, they will be published as soon as possible. Articles longer than eight printed pages may be published in two or more installments, unless the author is willing to pay the entire costs of a sufficient number of addi- tional pages in any one issue to enable such an article to appear without division. Editorial Policy: Manuscripts on taxonomy, systematics, morphology, physiology, ecology, behavior and similar aspects of insect life and related terrestrial arthropods are appropriate for submission to ENTOMOLOGICAL NEWS. Papers on applied, economic and regulatory entomology and on toxicology and related subjects will be considered only if they also make a major contribution in one of the aforementioned fields. (Continued on inside of back cover) Postmaster: // undeli verable, please send form 3579 to Howard P. Boyd, 232 Oak Shade Road, Tabernacle Twp., Vincentown, New Jersey 08088, U.SA. SECOND CLASS POSTAGE PAID AT VINCENTOWN, NEW JERSEY, 08088, U.S.A. Vol. 109, No. 5, November & December, 1998 297 CEROCOCCUS MICHAELI (HEMIPTERA: CEROCOCCIDAE): A NEW SPECIES OF FALSE PIT SCALE FROM NEW ZEALAND1 Paris Lambdin^ ABSTRACT: A new species of pit scale insect, Cerococcus michaeli, from New Zealand is de- scribed and illustrated. Cerococcus michaeli represents the second indigenous species to New Zealand and the eighth species from the Australian Region in the genus. This species is distin- guished from other species in the genus by the presence of an anal shield with a dorsal opening, spine-like apical setae on anal lobes, the occurrence of asteroform tubular ducts, and the absence of multilocular pores in transverse abdominal rows on the ventrum. A modified key is provided to separate C. michaeli from other known species in this taxon. Species of false pit scales (Cerococcidae) are represented in all major zoo- geographical regions, however, most are found in the tropical and subtropical areas. Several species are considered important pests of agricultural crops and ornamentals (Lambdin and Kosztarab 1977). Of the 56 species assigned to the genus Cerococcus, only one species, C. corokiae (Maskell), has been described from New Zealand. Recently, a new species was found while examining a series of unidentified scale insect material from the coccoidea collection of the National Museum of Natural History, Washington, D.C., USA. This species represents the eighth species known from the Australian Region in this genus. This new species is placed in the genus Cerococcus based on measure- ments and observations on 37 morphological characteristics of the adult fe- male. The structures evaluated are considered common to species in this genus (Borchsenius 1959, Danzig 1980, Lambdin & Kosztarab 1977). Specifically, the presence of dorsal 8-shaped pores, prominent anal lobes, a triangular anal shield, and cribriform plates are consistent structures for this taxon. In addi- tion, this species has a three-segmented labium, one segmented antennae, bilocu- lar pores, submarginal spiracles and associated spiracular furrows lined with quinquelocular pores on the ventrum. All measurements are rounded to the nearest micrometer. Where possible, 10 measurements of each structure were taken and are presented as an average followed by the range in parentheses. Terminology follows Lambdin and Kosztarab (1977). Cerococcus michaeli, NEW SPECIES (Fig. la-k) Type Locality. Coromandel, New Zealand. Type-Material. Holotype adult (encircled, lower center) and 4 paratype on 1 slide (No. 1651), on Dysoxylum spectabile, Coromandel, New Zealand, 1 Received: July 2, 1998. Accepted July 27, 1998. 2 Department of Entomology and Plant Pathology, University of Tennessee, Knoxville, TN. 3790 1 , ENT. NEWS 109(5) 297-300, November & December, 1998 298 ENTOMOLOGICAL NEWS Description of Slide-mounted Adult Females Body (Fig. la) pear shaped, membranous, 732 (650-820) long, 464 (410-540) wide; with distinct anal cleft; anal lobes (Fig. Ib) elongate, with scale-like overlapping cells, 88 (78-90), long, 49 (48-50) wide, each lobe with an outer (conical) and inner (needle-like) subapical seta, each 7 (6-12), and an stout apical seta (needle-like) 11 (10- 12) long. Dorsal Surface (Left half) Anal shield (Fig. la,b) triangular, with enclosed anterior anal opening, plate 69 (65-75) long, 54(52-55) wide. Anal ring (Fig. la,b) minute, 12(12-13) indiam., with 6 fleshy setae, each 18 (14-21 ) long. Cribriform plates (Fig. Ic) unevenly aerolated, 2 pairs, 1 pair each located on segments 7-8 anterior to anal lobes and triangular plate; each 5 (4-7) in diam. 8-shaped pores (Fig. Id) sparse, appearing to form a swirled pattern on derm with subcircular areas devoid of pores, each 4 (3-5) long, 2 (2-3) wide. Setae (Fig. le) sparse, few submedial tack-like setae in longitudinal rows, especially on 6th - 9th abdominal segments, each 2 (2-3) long, other setae rare. Simple disc pores absent. Tubular ducts (Fig. 1 0 asteroform, inner ductule reduced, invagi- nated inner end with 1 -3 minute teeth, outer ductule long and slender; scattered throughout derm, more numerous in marginal areas, especially in posterior abdominal region; each 21 (18-25) long, 1 (1-2) wide. Ventral Surface (Right Half) Antennae (Fig. Ig) one segmented, 10 (8-12) long, 9 (6-15) wide; with 6 (4-7) setae. No associated quinquelocular pores (Fig. Ih) at base. Bilocular pores (Fig. li) subcircular, irregu- larly spaced, most numerous in submarginal area of cephalothorax, especially around mouth- parts, antennae, and spiracles; few occasionally on margin of abdominal segments, each 4 (3-5) in diameter. Clypeolabral shield rectangular, 1 38 ( 1 34- 1 40) long, 115(112-118) wide. Marginal band of 8-shaped pores (Fig. Id) extending around body to apex of anal lobes and in transverse rows, one pore wide, on abdominal segments; each 4 (3-5) long, 2 (2) wide. Labium three-seg- mented, triangular, 54 (5 1 -58) long, 55 (5 1 -58) wide; with 5 (5-6) pairs setae, each 5 (3-6) long. Legs absent. Spiracles (Fig. Ij) located in submarginal area, 29 (28-31) long, 11 (11-12) wide, atrial diameter 4 (3-5); spiracles and spiracular furrows with associated quinquelocular pores, few 4-7 locular pores; anterior spiracle with lateral cluster of 9 (7- 1 1 ) pores, spiracular furrow with 2 1 ( 1 5-30) pores extending from spiracle to margin; posterior furrows bifid, cluster of 8 (6- 10) pores associated with spiracle, 7 (3-1 1 ) pores in anterior branch of spiracular furrow and 3 (2-5) pores in posterior branch, each pore 4 (4-5) in diam. Multilocular pores absent. Setae (Fig. Ik) sparse, tack-like, in segmental transverse rows, 3 pairs associated with vulva, 3 medial pairs between antennae, and a seta associated with each spiracle, each 2 (2-3) long. Tubular ducts (Fig. If) similar in shape and size to those on dorsum, but fewer on ventrum. Vulva large, ca. 50 in diam., largest among known cerococcids. Etymology. This species is named for my son, Michael, in tribute to his interests in insects. DISCUSSION Morphological similarities of C. michaeli to other species in the genus Cerococcus include: a similar body shape (pyriform), a long anal cleft and triangular anal shield, cribriform plates, and 8-shaped pores on the dorsum. Ventrally, this species has a three segmented labium, one segmented antennae, and a pair of thoracic spiracles with a bifid posterior furrow lined with quinquelocular pores. Also, the type of pores (bilocular, quinquelocular, and 8-shaped) and their arrangement on the ventrum are also similar to those of other species in the genus. This species is easily distinguished from other species by the presence of an asterolecaniid type of tubular duct. This is the first cerococcid known to have such tubular ducts. The long outer ductile has Vol. 109, No. 5, November & December, 1998 299 Fig. 1 . Cerococcus michaeli Lambdin, n. sp.: (a) dorsoventral view; (b) anal lobes and anal shield; (c) cribriform plate; (d) 8-shaped pore; (e) dorsal seta; (0 tubular duct; (g) antenna; (h) quinquelocular pore, (i) bilocular pore; (j) spiracle; (k) ventral seta. 300 ENTOMOLOGICAL NEWS been reduced, but there are one to three teeth within the cup. Also, the anal shield differs somewhat from those typically exhibited by other species of false pit scales. There exists an opening near the anterior margin of the anal shield for waste elimination and the shield curves ventrad, but does not join medially. The minute anal ring is enclosed by the anal shield and has six slen- der anal ring setae. Of the 56 known species in the taxa, only one other spe- cies, C. gallicolus Mamet, from Madagascar is known to have six setae. Also, there is a lack of quinquelocular pores at the base of the antennae and mul- tilocular pores on the ventral abdominal segments. In addition, these segments are distinguished by a transverse row of 8-shaped pores. The other endemic species to New Zealand, C. corokiae (Maskell), is dis- tinguished from this species by the presence of more numerous cribriform plates, an anal ring with eight setae, an anal shield without an opening, two sizes of 8-shaped pores, presence of a submarginal row of quinquelocular pores extending from the antennae to the posterior spiracles, and multilocular pores in transverse abdominal rows. C. michaeli possess some of the more primitive traits observed in species assigned to this genus (scale-like anal lobes, the un- evenly aerolated cribriform plates, and perhaps the tubular ducts). This new species may be identified using a modified key (Lambdin and Kosztarab 1977) to the adult females of Cerococcus as follows: 32. Without quinquelocular pores at base of each antenna 32a With quinquelocular pores at base of each antenna 33 32a. Posterior spiracular furrows absent; anal shield without dorsal opening; 1 -segmented leg stubs present; with multilocular pores in transverse abdominal rows .... indonesiensis Posterior spiracular furrows present; anal shield with dorsal opening; 1 -segmented leg stubs absent; without multilocular pores in transverse abdominal rows michaeli ACKNOWLEDGMENTS Grateful appreciation is extended to D. R. Miller, SELXARS USDA, Bldg. 046, BARC-W, Beltsville, MD 20705, for the loan of the material studied. I also thank R. Pereira, D. Paulsen, and Adrian Mayor, University of Tennessee, Knoxville, TN, for their reviews and comments on the manuscript. LITERATURE CITED Borchsenius, N. 1959. Notes on coccid fauna of China. 7. A new family of soft scales Lecaniodiaspididae. fam. n. (Homoptera: Coccoidea). (In Russian). Entomol. Obozr. 38: 840- 846. Danzig, E. M. 1980. Coccoids of the Far East of USSR (Homoptera, Coccinea) with a phyloge- netic analysis of the coccoid fauna of the world. (In Russian). Entomol. Obozr. 54:51-64. Lambdin, P. L. and M. Kosztarab. 1977. Morphology and systematics of the adult females of the genus Cerococcus (Homoptera: Coccoidea: Cerococcidae). Va. Polytech. Inst. State Univ. Res. Div. Bull. 128. 252pp. Vol. 109, No. 5, November & December, 1998 301 THE LARVA OF MACROTHEMIS INACUTA (ODONATA: LIBELLULIDAE)1 Rodolfo Novelo-Gutierrez^ , Alonso Ramirez^ ABSTRACT: A detailed description and illustrations of the larva of Macrothemis macula are provided. Larva is similar to M. celeno but can be distinguished by stouter movable hook, and larger lateral spines and dorsal portuberances on abdominal segments 8-9. Larvae were found living in lentic environments, in muddy areas close to the shore, where emerging and floating vegetation was present. The Neotropical genus Macrothemis comprises 37 species described to date (Garrison, in litt.) of which only nine occur in Mexico and Central America (Paulson, 1982; Gonzalez-Soriano & Novelo-Gutierrez, 1996). Despite the fact that it is a very speciose genus, its immature stages are poorly known; larvae of only three species have been described: M. celeno (Selys) (Klots, 1932), M. musiva Calvert (Santos, 1970), and M. pseudimitans Calvert (Limongi, 1989). Here, we describe and illustrate the larva of M. inacuta Calvert. Terminology of the labium follows Corbet (1953). Macrothemis inacuta Calvert (Figs. 1-9) Description: Exuviae yellowish-brown, larvae brown; body short and robust; integument covered with small spiniform setae. Head: Two times wider than long, narrowed posteriorly, occipital margin slightly con- cave, cephalic lobes poorly developed, not bulging (Fig. 1), covered with minute spiniform setae and long delicate setae; compound eyes rather small, slightly more dorsal than lateral. Antennae 7-segmented (Fig. 2), the third the longest, relative length of antennomeres: 0.5, 0.7, 1 .0, 0.6, 0.7, 0.8, 0.8; scape mostly pale with a dark dorsobasal spot, pedicel pale with a dark ring on distal end; third antennomere reddish-brown, antennomeres 4-6 dark in basal half, pale in apical half; last antennomere mostly pale with apex dark. Labrum bare, setose on distal border; clypeus bare. Frons and vertex with abundant spiniform setae and long and delicate setae. Mandibles biramous (Fig. 3), external branch with four cusps in both mandibles and a small cusp at base of ventral cusp on right mandible, internal branch vestigial, represented only by three, low, blunt protuberances slightly more developed on right mandible. Maxillae: Gale- olaciniae with seven acute teeth (Fig. 4), four large and three smaller, palp with numerous, long, stiff setae, ending in a robust spine. Labium: Prementum-postmentum articulation reach- ing posterior margin of mesocoxae; prementum subrhomboid with 7+3 and 7+2 setae (Fig. 5a), lateral margins with small spiniform setae, ligula prominent with distal margin very slightly serrate and with a row of stout setae on dorsal surface, but very close to distal margin, its tip . 1 Received December 4, 1997. Accepted February 25, 1998. 2 Institute de Ecologia, A.C. Departamento de Entomologia. Apartado Postal 63, 91000 Xalapa, Veracruz, MEXICO. E-mail: novelor@ecologia.edu. mx -* Institute of Ecology, University of Georgia, Athens, GA 30602, U.S.A. E-mail: aramirez@ arches.uga.cc.uga.edu ENT. NEWS 109(5) 301-306, November & December, 1998 302 ENTOMOLOGICAL NEWS PLATE I. Figs. 1-4, Macrothemis inacuta larva. 1) Dorsal habitus of last instar larva (left legs omitted); 2) Left antenna, dorsal view; 3) Mandibles, ventrointernal view: a, left mandible, b, right mandible; 4) Ventral view of right maxilla (a), ventrointernal aspect of the dorsal teeth (b). Vol. 109, No. 5, November & December, 1998 303 Labial palp with six long setae and a row of short, robust, spiniform setae on basal 0.70 of outer margin (Fig. 5c); movable hook robust, incurved, suddenly sharply-pointed, as long as palpal setae (Fig. 5a); distal margin of palp with seven crenations, notches between crenations deep, diminishing in depth from outer margin to internal one, each crenation finely serrate and bearing three stout setae, one large and two small, except the two dorsal crenations which bear only two (Fig. 5c); inner margin of palp with a single row of stout and stiff setae; a group of 7-9 setellae at base of palp close to articulation with prementum; surface of palp sprinkled with dark spots of different sizes in an irregular pattern. Thorax light brown; lateral and posterior margins of pronotum rounded; pronotal disk yellow with an inverted, dark V-shaped spot on middle part (Fig. 1 ); anterior margin of proepisternum with a tuft of long stiff setae; proepimeron with a longitudinal reddish-brown stripe on its upper margin. Synthorax mostly dark, pale on sutures. Anterior and posterior wing pads dark, their tips darker brown, reaching and surpassing posterior margin of abdominal segment five respectively. Legs long (e.g. hind legs when fully extended reaching beyond the level of anal pyramid); meso- and metacoxae with a distal, ventrolateral, digitiform process (Fig. 6a); femora slightly compressed laterally, dorsal and external surfaces covered with long, stiff setae intermingled with spiniform ones; femora and tibiae pale, with a clear pattern of three transverse bands at basal, middle and distal portions; tarsi yellow, dark on apical 0.50 of third tarsomere; claws simple with a pulvilliform empodium. Abdomen reddish-brown, with a complex color pattern shown on Fig. 1; tergites 3-9 with spine-like protuberances well developed, that on 3 almost vertical, remainder gradually di- rected rearward (Fig. 7a); lateral margins of 2-3 slightly convex, straight on 4-7 and slightly concave on 8-9; those of 2-7 covered with minute spiniform setae which increase in length and robustness on 8-9; lateral spine on 8 0.65 as long as dorsal length of 8; that on 9 as long as or longer than dorsal length of 9, reaching level of tips of cerci (Fig. 8). Gonapophyses indistin- guishable, just a minute gonopore visible in male. Epiproct and paraprocts pyramidal, acutely pointed, with small spiniform setae on margins; epiproct little longer than its basal width (ratio 1:0.8), in lateral view (Fig. 9), the basal 0.50 of its dorsal margin slightly concave then becom- ing straight but slightly slanting. Cerci sharply pointed, shorter than remaining appendages. Epiproct and basal 0.35 of cerci dark, distal 0.65 of cerci and all of paraprocts pale. Size pro- portions: Epiproct 1 .0, paraprocts 1 .0, cerci 0.80. Dimensions (mm): Total length including appendages 17-17.6; abdomen 10.2-10.6; hind femur 4.2-4.4; maximum width of head 4.5-4.6; lateral spines on abdominal segment 8, 0.5; on 9, 0.6-0.7. Material examined: 2 exuviae (Cf Cf ), reared, 5 last instar larvae (4Cf Cf, 1 9 )• MEXICO: Veracruz; Emiliano Zapata, Miradores (Lagoon), 1000 meters above sea level (masl), 8-IX-1996, R. Arce leg., (5Cf Cf , 1 9 ) as last instar larvae. Deposited at Institute de Ecologfa, A.C. Xalapa, Mexico. COSTA RICA: Provincia de San Jose; Zona Protectora El Rodeo, 16-XI-1990, A. Ramirez leg., (ICf ) as last instar larva. Deposited at Museo de Zoologia, Universidad de Costa Rica. DISCUSSION The larva of M. inacuta closely resembles that of M. celeno in the number of palpal setae (6), size proportions on caudal appendages, and general stat- ure. It differs by the larger and stouter movable hook on the labial palp, coxal processes more developed, larger lateral spines on abdominal segments 8-9, and larger dorsal protuberances on 3-9 (mainly those on 8-9). The larva of M. inacuta differs from that of M . pseudinritans by the stouter movable hook on the labial palp, the larger metacoxal digitiform process (cf. Fig. 6), and the shape of the dorsal protuberances on 7-9 (cf. Fig. 7). The larva of M. musiva 304 ENTOMOLOGICAL NEWS o> 00 LATE II. Figs. 5-9. Morphology of Macrothemis larvae. 5) Dorsal view of prementum (a), detail of ligula (b), and dorsointemal view of right palp (c) of M. inacuta, 6) Left metacoxae of M. inacuta (a) and M . pseudimitans (b), showing metacoxal process (mcp), lateroventral view; 7) Profile view of abdominal dorsal line of M. inacuta (a) and M. pseudimitans (b); 8) Partial figure of abdominal segments 8-10 showing lateral spines on 8-9, dorsal view; 9) Left lateral aspect of Vol. 109, No. 5, November & December, 1 998 305 differs in having only 8 premental setae on each side of middle (5+3) and only 4 palpal setae. The genus Macrothemis has not been clearly defined based upon larvae; this is important in order to differentiate Macrothemis from the closely related genus, Brechmorhoga. Needham and Westfall ( 1 955) tabulated some features based exclusively on M. celeno, but this does not constitute a generic characterization. At present, these genera can be differentiated by the following combination of features (those of Brechmorhoga [cf. Novelo- Gutierrez, 1995] in parentheses): Integument mainly setose (granulose), cepha- lic lobes widely rounded, not bulging (bulging); pronotum with an inverted V-- shaped dark mark (without such a mark); inferior margin of proepimeron with long and delicate setae (with short and robust setae); sides of abdomen convex throughout (parallel on segments 5-7); abdominal segment 10 and anal pyra- mid hidden laterally by the lateral spines of segment 9 (segment 10 and anal pyramid not hidden [partially hidden in B. rapax] by spines of 9); posterior margin of sternite 10 usually visible in dorsal view (not visible from above); dorsal protuberances well developed on abdominal segments 3-9, often acutely- pointed (well developed on 2-5 or 2-6, vestigial or lacking on posterior seg- ments, often bluntly-tipped); cerci 0.75-0.80 as long as epiproct (cerci 0.50- 0.66 as long as epiproct). Ecological notes.- Larvae of M. inacuta were found in a lagoon in open pasture land, on the muddy bottom near shore where scattered aquatic vegeta- tion grew. Mature larvae were caught on September 8, 1996. The lagoon was visited one year later (September 21, 1997) but no M. inacuta larvae were found, although teneral adults were captured in surrounding areas. These pre- liminary observations suggest potential synchrony (or seasonality?) in emer- gence of the larvae, probably starting at the end of August or beginning of September. Available published information indicates that all Macrothemis species dwell in lotic habitats (Garcia-Diaz, 1938; Limongi, 1989; Gonzalez- Soriano, 1992). However, Santos (1970) mentioned that Macrothemis musiva larvae probably inhabit intermediate lotic and lentic waters. M. inacuta is an- other exception since larvae were collected in lagoons, and adults are seen at streams and rivers. It is likely that when living in a lotic system the larvae favor still waters in pools and areas near the margins. ACKNOWLEDGMENTS We thank Roberto Arce-Perez, M. Sc. for his invaluable help collecting and maintaining alive larvae of M. inacuta. We also thank Sidney Dunkle (Piano, TX) and Michael May (New Brunswick, NJ) for their invaluable criticism to improve the final manuscript. A. Ramirez was supported by the National Science Foundation grant DEB95-28434 during preparation of the manuscript. LITERATURE CITED Corbet, P.P. 1953. A terminology for the labium of larval Odonata. The Entomologist 86: 191- 196. 306 ENTOMOLOGICAL NEWS Garcia-Diaz, J. 1938. An ecological survey of the fresh water insects of Puerto Rico. 1. The Odonata: with new life-histories. J. Agric. Univ. Puerto Rico 22(1 ): 43-97. Gonzalez-Soriano, E. 1992. Macrothemis ultima spec, nov., a new dragonfly from the state of Jalisco, Mexico (Anisoptera: Libellulidae). Odonatologica 21(l):91-95. Gonzalez-Soriano, E. y R. Novelo-Gutierrez, 1996. Odonata, pp. 147-167. In: J. Llorente- Bousquets, A.N. Garcia-Aldrete y E. Gonzalez-Soriano (eds.). Biodiversidad, taxonomia y biogeografia de artropodos de Mexico: Hacia una sintesis de su conocimiento. UNAM, Mexico. Klots, E.B. 1932. Insects of Porto Rico and the Virgin Islands. Odonata or Dragon Flies. Scien- tific Survey of Porto Rico and the Virgin Islands 14: 1-107. Limongi, J.E. 1989. Estudio morfo-taxonomico de nayades de algunas especies de Odonata (Insecta) en Venezuela (II). Memoria 49 ( 1 3 1 - 1 32):405-41 9. Novelo-Gutierrez, R. 1995. La nayade de Brechmorhoga praecox (Hagen, 1 861 ), y notas sobre las nayades de B. rapax Calvert, 1898, B. vivax Calvert, 1906 y B. mendax (Hagen, 1861)(Odonata: Libellulidae). Folia Entomol. Mex. 94:33-40. Paulson, D.R. 1982. Odonata, pp. 249-277. In: S.H. Hurlbert & A. Villalobos-Figueroa (eds.), Aquatic Biota of Mexico, Central America and the West Indies. San Diego State University, San Diego, California. Santos, N.D. 1970. Descricao da ninfa de Macrothemis musiva (Hagen, 1861) Calvert, 1898 (Odonata: Libellulidae). Atas Soc. Biol. Rio de Janeiro 13:157-158. Vol. 109, No. 5, November & December, 1998 307 A NEW SPECIES OF THE NEOTROPICAL GENUS BYTHONIA (HOMOPTERA: CICADELLIDAE) AND THE FEMALE OF B. CON SENS A1 Marcio Felix^, Gabriel MejdalanP ABSTRACT: Bythonia ferruginea, new species, is described from the states of Bahia and Minas Gerais, Brazil. The female of B. consensa is described for the first time based on specimens from the states of Rio de Janeiro (new record) and Espirito Santo, Brazil. The presence of two sclero- tized plates from the sternum VIII is reported in females of Bythonia for the first time. Notes on specimens of B. kalypso from the Brazilian states of Santa Catarina (new record) and Minas Gerais are added. A map showing the known distribution of the species of Bythonia is also pre- sented. Three species of the Neotropical genus Bythonia Oman were recorded by Blocker and Webb (1990). Only a few specimens of this genus were cited in the literature. B. rugosa (Osborn, 1923), the type-species, is known only from the female holotype from Bolivia and a male from Peru (Linnavuori 1959). This species was originally described in the genus Nionia Ball. B. kalypso Linnavuori, 1959 and B. consensa Blocker and Webb, 1990 were, until the present paper, known only from their male holotypes from Brazil. The affini- ties of Bythonia to other leafhopper genera were briefly discussed by Blocker and Webb (1990), who assigned this genus to the subfamily lassinae. The origi- nal description by Oman (1936) and subsequent descriptions by Linnavuori (1959) and Blocker and Webb (1990) should be consulted for characteristics of the genus. A new species of Bythonia from the states of Bahia and Minas Gerais, Brazil, is herein described. The previously unknown female of B. consensa is described for the first time. This species is newly recorded from the state of Rio de Janeiro, Brazil. Notes on six additional male specimens of B. kalypso Linnavuori are added and the species is newly recorded from the state of Santa Catarina, Brazil. Acronyms for collections in which the specimens herein studied are de- posited are as follows: DZRJ (Departamento de Zoologia, Universidade Fe- deral do Rio de Janeiro, Rio de Janeiro, Brazil), MNRJ (Museu Nacional, Rio de Janeiro), and NHM (The Natural History Museum, London). In quotations of label data, a virgule (/) separates lines on a label and a semicolon separates information on different labels. Morphological terminology follows mainly 1 Received January 22, 1998. Accepted February 25, 1998. 2 Departamento de Zoologia, Institute de Biologia, Universidade Federal do Rio de Janeiro (UFRJ), Caixa Postal 68044, 21944-970 Rio de Janeiro RJ, Brasil. 3 Departamento de Entomologia, Museu Nacional, UFRJ, Quinta da Boa Vista, Sao Cristovao, 20940-040, Rio de Janeiro RJ, Brasil. E-mail: mejdalan@acd.ufrj.br. ENT. NEWS 109(5): 307-314, November & December, 1998 308 ENTOMOLOGICAL NEWS Young (1977). The software package FishMap (Buckup 1995) was used for producing a distribution map of the species of Bythonia. Bythoniaferruginea, NEW SPECIES (Figs. 1-7) Diagnosis. - Males of B. ferruginea can be distinguished from the other known species of the genus by the following features: pronotum with arrow-shaped group of irregular black spots; sternum VIII with anterior and posterior acute processes on lateral margins; pygofer without processes, vent- rolateral portion with longitudinal fold; basal half of styles with blunt dorsal process; aedeagus with pair of lateral processes on apical third and without median spine on apodeme. Description of male. - Length including forewings, 6.3-6.8 mm. Head (Fig. !) short, me- dian length of crown approximately one-eighth interocular width, anterior margin rounded; sur- face of crown with small punctures; face with pubescence; clypeus with inconspicuous muscle impressions; clypellus in lateral view forming angle at transition with clypeus; antennal ledges slightly protuberant; lora broad, slightly striate; genae with deep excavation below eyes. Thorax (Fig. 1 ) with pronotal width approximately equal to transocular width of head; disc of pronotum with pubescence, transversely striate; dorsopleural carinae complete; mesonotum transversely striate. Forewings with clavus and adjacent regions of corium punctate. Legs pubescent. Ab- dominal sternum VIII (Fig. 2) broad in ventral view, lateral margins with anterior and posterior small acute processes, posterior margin broadly concave, ventral surface with microsetae uni- formly dispersed. Remaining morphological characteristics of head and thorax as in the original generic description of Oman (1936: 358) and the subsequent description of Linnavuori (1959: 13). Color. - Reddish-brown marked with black. Crown (Fig. 1) almost entirely black; ocelli yellowish. Pronotum (Fig. 1 ) with arrow-shaped group of irregular black spots; mesonotum (Fig. 1 ) with pair of subtriangular black maculae on anterior portion and median black stripe continu- ous with pronotal arrow-shaped mark. Forewings hyaline, with irregular amber areas. Hindwings hyaline. Clypeus and clypellus with median black stripe narrowing toward inferior portion; lora with blackish spot. Legs with blackish markings. Male genitalia. - Pygofer (Fig. 3) elongate in lateral view, with apex rounded, ventrolateral portion with longitudinal fold, apical half of disc with numerous microsetae, apical margin with macrosetae extending anteriorly along one-third of ventral and dorsal margins. Subgenital plates (Fig. 4) in lateral view elongate, extending posteriorly beyond apex of pygofer, median portion slightly enlarged, apex rounded, plates with dispersed microsetae. Styles (Fig. 5) in lateral view with apical portion curved dorsally, bifurcate, C-shaped, with short triangular process and small group of long microsetae, dorsal margin with long microsetae, basal half with dorsal blunt pro- cess. Connective (Fig. 6) in dorsal view with very short, dorsally curved arms, dorsal area of stalk with median keel. Aedeagus (Fig. 7) curved dorsally in lateral view, with apical digitiform process, distal third with pair of thin lateral processes; aedeagal apodeme well developed, apex bifurcate. Female unknown. Known distribution. - Brazilian states of Bahia and Minas Gerais (Fig. 1 4). The two known records of B. ferruginea are included in areas originally covered by the Brazilian Atlantic Forest (see map in Warren 1996). Vol. 109, No. 5, November & December, 1998 309 Figs. 1-7. Bythonia ferruginea, new species, male. 1, Head and thorax, dorsal view. 2, Abdomi- nal sternum VIII, ventral view. 3, Pygofer, lateral view. 4, Subgenital plate, lateral view. 5, Style, lateral view. 6, Connective, dorsal view. 7, Aedeagus, lateral view. Etymology. - The species epithet ferruginea, is of Latin derivation and refers to the reddish-brown color of its anterior dorsum. Type material. - Holotype: Male, Brazil, "Encruzilhada-B A [state of Bahia, 1 5° 3 11 S, 40° 54' W]/ XI-1972/ Alvarenga/ 960 m", MNRJ. Paratypes: Two males, same data as holotype, MNRJ and DZRJ; three males, Brazil, "Pedra Azul/ Minas [state of Minas Gerais, 16° 00' S, 41° 17' W], Brasil; Seabra &/ Oliveira/ XI-72", MNRJ. Notes. - The color pattern in B. ferruginea is very similar to that of B. consensa (see description below). The anterior dorsum (Fig. 1) in both species is reddish-brown with black markings and the face has a median black stripe. In terms of morphology, the sternum VIII and male genitalia of B. ferruginea 3 1 0 ENTOMOLOGICAL NEWS are also similar to those of B. consensa. The sternum VIII (Fig. 2) in the new species presents an anterior and a posterior pair of lateral processes, while in B. consensa only a single median pair of processes is present. The pygofer (Fig. 3) in B.ferruginea has a ventrolateral longitudinal fold, a feature that is not observed in B. consensa. On the other hand, the ventral margin of the pygofer in the latter species has a bifurcate process which is not present in the former. The apical portion of the styles (Fig. 5) in both species is curved dor- sally and bifurcate, but the basal half of this structure in B. ferruginea has a blunt projection which does not occur in B. consensa. The aedeagus (Fig. 7) in these species is curved dorsally and bears a pair of lateral processes. These processes are longer in B. ferruginea. The aedeagal apodeme in B. consensa has a spine that is not present in B. ferruginea. Bythonia consensa Blocker and Webb (Figs. 8-13) Description of female.- Length including forewings, 7.6 mm. Morphological characteris- tics of head and thorax as in B.ferruginea, new species, and also as in the generic descriptions of Oman (1936: 358) and Linnavuori (1959: 13) and the original specific description of Blocker and Webb (1990: 294). Color. - Reddish-brown marked with black, ocelli yellowish. Pronotum (Fig. 8) with small blackish areas and faint, longitudinal blackish-brown stripe on median portion; mesonotum (Fig. 8) with pair of black maculae on anterior portion and faint, longitudinal blackish-brown stripe on median portion continuous with pronotum stripe, extending to transverse sulcus. Forewings red- dish-brown with apex amber. Hindwings hyaline. Clypellus with small black maculae on central portion. Legs with blackish areas. Female genitalia.- Abdominal sternum VII (Fig. 9) narrowed posteriorly, ventral surface strongly convex, with microsetae uniformly distributed, lateral margins without processes, pos- terior margin shallowly emarginate on each side of median quadrangular concavity, lateral mar- gins of emarginations with spiniform processes. Sternum VIII (Fig. 10) well developed, sclero- tized, formed in dorsal view by pair of plates, each with deep posterior concavity. Pygofer (Fig. 1 1 ) elongate in lateral view, with apex truncate, apical half with microsetae uniformly distrib- uted, ventroapical portion with small group of macrosetae. Ovipositor with first valvulae long and narrow, apex blunt, dorsal and ventral sculptured areas with sinuate and almost vertically aligned striae; second valvulae (Fig. 12) slightly expanded apically, without preapical promi- nence, apex blunt, apical portion with broad, rectangular, sloping teeth, median portion (Fig. 13) with small irregular denticles. Known distribution. - B. consensa was originally described by Blocker and Webb ( 1 990) from the state of Espirito Santo. This species is herein newly recorded from the state of Rio de Janeiro (Fig. 14). The known records of B. consensa are included in areas originally covered by the Atlantic Forest. Material examined. - One female, two males, Brazil, "Angra dos Reis [state of Rio de Janeiro, 23° 00' S, 44° 19' W]/ Japuhyba/ 2-1944/ Wygodzinsky L.; MNRJ", MNRJ. Two fe- males, Brazil, "Corrego Ita [Corrego Ita]/ E. Santo, [state of Espirito Santo] Br./ X-1954/ W. Zikan", MNRJ. Male holotype, "Tijuco Preto/ Esp. Santo [state of Espirito Santo, 20° 17' S, 40° 53' W]; Holotype/ Bythonia consensa/ Blocker + Webb/ 1990", NHM. Notes. - The two above-mentioned males of B. consensa from the state of Vol. 109, No. 5, November & December, 1998 311 Figs. 8-13. Bythonia consensa, female. 8, Head and thorax, dorsal view. 9, Sternum VII, ventral view. 10, Sternum VIII, dorsal view. 1 1, Pygofer, lateral view. 12, Second valvula of ovipositor, lateral view. 13, Area between arrows in figure 12 at a higher magnification, lateral view. Rio de Janeiro were identified using the original description of Blocker and Webb (1990) and through the examination of the species' male holotype in the NHM. The reddish-brown color of the anterior dorsum of the females of B. consensa is similar to that of the males. However, the latter have a longitudinal black stripe on clypeus and blackish areas on crown and anterior portion of pronotum that are not observed in the females. B. rugosa is the only other species in the genus Bythonia for which the female has been described. B. consensa can be distinguished from B. rugosa by the form of the sternum VII. In the former species this sternum (Fig. 9) does not have processes on lateral margins and a quadrangular concavity is present on posterior margin, while in the latter species it has a pair of lateral spiniform processes and a posterior trapezoidal process (see Blocker and Webb 1990). 3 1 2 ENTOMOLOGICAL NEWS Females of B. consensa present two sclerotized plates at the base of the ovipositor dorsad of the sternum VII (Fig. 10). These plates, which are here reported in the genus Bythonia for the first time, are homologous to those described by Nielson (1965) in the proconiine genus Cuerna Melichar and to the sclerites of the genital chamber described by Young (1977) in several cicadelline genera. They are derived from the sternum VIII, which is greatly reduced in females of cicadellids (Nielson 1965, Mejdalani in press). Nielson (1965) demonstrated that this modified sternum can provide useful character- istics for distinguishing species in Cuerna. Comparative studies on females of Bythonia are necessary in order to know if the sternum VIII is also of taxo- nomic value in this genus. The sternum VIII in B. consensa is similar to the derived type of modified sternum described by Nielson (1965) in Cuerna, which is characterized by the presence of two distinct plates. The first and second valvulae of the ovipositor of B. consensa are very similar to those of B. rugosa. The valvulae of the latter species were described by Hill (1970). The dorsal sculptured area of the first valvulae presents in both species sinuate, almost vertically aligned striae. These striae differ greatly from the alveolate sculpturing found in other lassinae (see Hill 1 970, Dietrich 1 993). The shaft of the second valvulae in B. consensa (Fig. 1 2) and B. rugosa, unlike those of other lassinae (see Hill 1970), does not present dorsal tooth-like promi- nences. The apical portion of these valvulae is slightly expanded and bears teeth in both species. Bythonia kalypso Linnavuori B. kalypso was described from a single male from the state of Minas Gerais (Linnavuori 1959: 15). We have identified five additional male specimens of this species from Minas Gerais in the MNRJ collection; one male specimen from the Brazilian state of Santa Catarina was identified in the NHM collec- tion (Fig. 14). This is the first record of B. kalypso from Santa Catarina. This species also occurs in areas of Atlantic Forest. In terms of color and morphol- ogy, these specimens agree fairly well with the original description of the spe- cies. However, they are slightly smaller (7.5-8 mm) than the holotype (9 mm). Males of this species can be recognized by the following characteristics: pygofer with a triangular projection on median portion of ventral margin; apex of styles acute; apex of aedeagus with an acute process directed anteriorly and a blunt, weekly sclerotized process directed dorsally. The holotype of B. kalypso is reportedly deposited in the Hungarian Natural History Museum but could not be located in that collection (Blocker and Webb 1990). Material examined. - Five males, Brazil, "Pedra Azul/ Minas [state of Minas Gerais, 16° 00' S, 41 ° 1 T W], Brasil; Seabra &/ Oliveira/ XI-72; MNRJ", MNRJ. One male, Brazil, "Colesao/ Campos Seabra; CorupaV S. Catarina [state of Santa Catarina, 26° 25' S, 49° 14' W] Brasil/ I-1954/A. Mailer", NHM. Vol. 109, No. 5, November & December, 1998 313 Bytfionja rugosa • Bytnonja consensa ^ Bytfionja tcaJypso • Bytfionja ferruginea Fig. 14. Known geographic distribution of the species of the South American genus Bythonia. B. rugosa was also recorded from an unknown locality in Peru by Linnavuori (1959). ACKNOWLEDGMENTS The manuscript benefited from the useful comments of A. L. Carvalho, L. B. N. Coelho, L. F. M. Dorville, M. W. Nielson, S. A. Vanin, M. D. Webb, and two anonymous reviewers. This work was finished during a visit of GM to the NHM. Working facilities in that museum arranged by M. D. Webb, J. Margerison-Knight, and J. Martin are greatly acknowledged. Fellowships from Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES, Brazil) to MF and from Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP, Brazil) to GM are acknowledged. LITERATURE CITED Blocker, H. D. and M. D. Webb. 1990. The leafhopper genus Bythonia (Homoptera: Cicadellidae). Entomol. News 101: 293-296. Buckup, P. A. 1995. FishMap, a software package for producing species distribution maps. Version 1.8. User's guide. Published by the author, Rio de Janeiro. Dietrich, C. H. 1993. A new genus of lassinae from southeastern Brazil (Homoptera: Cicadellidae). Proc. Entomol. Soc. Wash. 95: 475-480. Hill, B. 1970. Comparative morphological study of selected higher categories of leafhoppers (Homoptera: Cicadellidae). Unpublished Ph. D. dissertation, North Carolina State Univer- sity, Raleigh. 3 1 4 ENTOMOLOGICAL NEWS Linnavuori, R. 1959. Revision of the Neotropical Deltocephalinae and some related subfami- lies (Homoptera). Ann. zool. Soc. Zool. Bot. Fenn. 'Vanamo' 20: 1-370. Mejdalani, G. In press. Morfologia externa dos Cicadellinae (Homoptera: Cicadellidae): comparacao entre Versigonatia ruficauda (Walker) (Cicadellini) e Tretogonia cribrata Melichar (Proconiini), com notas sobre outras especies e analise da terminologia. Revta bras. Zool. Nielson, M. W. 1965. A revision of the genus Cuerna (Homoptera, Cicadellidae). Tech. Bull. U. S. Dep. Agric. 1318: 1-48. Oman, P. W. 1936. A generic revision of American Bythoscopinae and South American Jassinae. Univ. Kans. Sci. Bull. 24: 343-420. Osborn, H. 1923. Neotropical Homoptera of the Carnegie Museum. Part 2. Records and descrip- tions of five new genera and sixty-five new species of the subfamily Jassinae. Ann. Cam. Mus. 15: 27-79. Warren, D. 1996. A ferro e fogo. A historia e a devastacao da Mata Atlantica brasileira. Com- panhia das Letras, Sao Paulo. Young, D. A. 1977. Taxonomic study of the Cicadellinae (Homoptera: Cicadellidae). Part 2. New World Cicadellini and the genus Cicadella. Bull. N. Carol. Agric. Exp. Stn. 239: 1-1 135. BOOKS RECEIVED AND BRIEFLY NOTED BUTTERFLY CONSERVATION. (Second ed.) T.R. New. 1998. Oxford Univ. Press. 248 pp. $27.95 paperback). This book provides a broad survey of the science of insect and butterfly conservation and the rationale for conserving both. Most of the book explores butterfly conservation and international efforts to safeguard species, including steps individuals can take to encourage and document but- terfly conservation. TERMITES. BIOLOGY AND PEST MANAGEMENT. M.J. Pearce. 1998. Oxford Univ. Press. 172 pp. 32 plates. $65.00 (cloth). This book provides a general scientific introduction to termites, including their biology, behavior, pest status, and control. It is directed to advanced students in entomology and pest man- agement, as well as to professionals. A FIELD GUIDE TO COMMON TEXAS INSECTS. B.M. Drees & J.A. Jackman. 1998. Gulf Publishing Co., Houston, TX. 359 pp. 381 color photos on 64 plates. $18.95 (paper). A regional field guide describing insects, mainly by order and family, and, in the larger orders, by genus, with specific examples. Vol. 1 09, No. 5, November & December, 1 998 3 1 5 PERLESTA GOLCONDA (PLECOPTERA: PERLIDAE), A NEW STONEFLY SPECIES FROM ILLINOIS1 R. E. DeWalt2, Bill P. Stark3, M. A. Harris4 ABSTRACT: The male, female, and egg of a new species of perlid stonefly, Perlesta golconda are described. Most specimens were captured from the banks of the Ohio River at Golconda, Illinois. This species is most similar to P. lagoi Stark, but may be differentiated by wing and body coloration, the male internal genitalia, and egg morphology. The addition of P. golconda brings to 16 the total number of known Perlesta species. Stark (1989) recognized Perlesta placida (Hagen) as a species complex nearly a decade ago. He described seven new species and revised and keyed another five species from eastern North America. Shortly thereafter, Poulton and Stewart (1991) added P. fusca from Arkansas. Recently, Kirchner and Kondratieff (1997) described P. teaysia from Virginia, and Stark and Rhodes (1997) added P. xube from Nebraska. During statewide monitoring of sensi- tive aquatic insects in Illinois, this new species was collected from the banks of the Ohio River near Golconda, Illinois. Perlesta golconda DeWalt and Stark, NEW SPECIES Male. Forewing length from 10.5 to 11 mm. General color pale yellow-brown. Ocellar patch diffuse mesally, but with dark bars almost connecting lateral ocelli to anterior ocellus (Fig. 1). Wing membrane pale amber with intercostal margin paler, veins light brown. Femora pale yellow on anterior face, but dark brown on dorsum (Fig. 3). Paraprocts short, subapical spine prominent (Figs. 2, 5). Tergum 10 sensilla basiconica patch sparse (Fig. 2). Penis tube and sac moderately long and sinuate (Fig. 6); dorsal patch broad and with narrow lateral bands of larger seta-like spines giving a distinctly darker aspect (Fig. 7). Caecum poorly developed; ventral base of sac with a small nipple (Fig. 6). Ventral extension of aedeagus was only par- tially everted (Fig. 6), but appeared to be long and narrow. Female. Forewing length from 13 to 14 mm. Body and wing coloration similar to the male. Subgenital plate lobes rounded, separated by a V-shaped notch; lobes with about 10 scattered large bristles each (Fig. 4). Egg. The two females produced only poorly sclerotized eggs, but they were sufficiently developed to demonstrate a smooth, to slightly granular chorion (Fig. 8). The egg collar was sessile and the anchor fibrous. Nymph. Unknown. Types. The holotype male and paratypes consisting of one male and two females were deposited at the Illinois Natural History Survey (INHS), Champaign, Illinois. Type locality. The holotype male and a male and female paratype were collected from the Ohio River at Golconda, Pope County, Illinois, on 25 June 1997. Universal transverse mercator coordinates are zone 16, 368,710 m easting, and 3,600,000 m northing (1927 North American 1 Received January 22, 1998. Accepted February 25, 1998. 2 Center for Biodiversity, Illinois Natural History Survey, 607 E. Peabody Dr., Champaign, IL 61820. 3 Biology Department, Mississippi College, Clinton, MS 39058 4 United States Geological Survey, 221 N. Broadway Ave., Urbana IL 61801 ENT. NEWS 109(5): 315-317, November & December, 1998 316 ENTOMOLOGICAL NEWS Scale, see legend 8 Fig. 1-8. Perlesta golconda : 1. Male head and pronotum. 2. Male tergum 10, sb - sensilla basiconica, p=paraproct. 3. Male right fore femur. 4. Female sternum 8. 5. Male paraproct, lat- eral, s=spine. 6. Aedeagus, lateral, c=caecum, dp=dorsal patch, n=nipple, v-ventral extension. 7. Aedeagus, dorsal. 8. Egg. Scales: 0.6 mm (1), 0.3 mm (2-4), 0.15 mm (5-8). Vol. 1 09, No. 5, November & December, 1 998 317 Datum). They were collected from a black light trap (2030-21 15 CST, 26.1°C air temperature) by R. E. DeWalt and L. J. Peraino. Another paratype female was taken from along the Ohio River in Elizabethtown, Hardin County, Illinois, 30 June 1993, by M. A. Harris and H. E. Kitchel. Etymology. Perlesta golconda is named for the town along the Ohio River where the majority of specimens were collected. DISCUSSION Perlesta golconda most closely resembles P. lagoi Stark. It may be differ- entiated from the latter by its lighter wing and body coloration, by the dorsal setal patch of the aedeagus forming two prominent, lateral bands (Fig. 6, 7), by the absence of egg follicular cell impressions, and by a sessile egg collar (Fig. 8). Perlesta lagoi has the typical dark brown wings of the genus, a dorsal patch composed of a single, wide row of spines, and the egg has a button-like collar and wide, circular, follicular cell impressions (Stark 1989). Additional specimens of P. golconda may demonstrate the usefulness of the pigment pat- terns of the ocellar patch and fore femora for identification. No nymphs of P. golconda were available for comparison to its congeners. Associated species in the light trap collection were the stonefly Isoperla bilineata (Say), the mayflies Hexagenia bilineata (Say), Stenacron interpunctatum (Say), Leucrocuta sp., Stenonema sp., the caddisflies Hydroptila waubesiana Betten, Potamyia flava (Hagen), Neureclipsis crepuscularis (Walker), Hydropsyche orris Ross, Nectopsyche Candida (Hagen), Ceraclea sp., and the megalopteran Neohermes sp. Two species of Perlesta are now known from Illinois. The other species, P. decipiens (Walsh), occurs statewide and was reported by Harris and Webb (1995). ACKNOWLEDGMENTS We thank D. W. Webb, S. J. Taylor, G. A. Levin, all of the 1NHS, for reviewing earlier drafts. Appreciation is extended to C. E. Warwick for scanning the original drawing and adding labels. Travel expenses and publication costs were provided by the Illinois Department of Natural Resources, Critical Trends and Assessment Project, a statewide monitoring program. LITERATURE CITED Harris, M. A., and D. W. Webb. 1995. The stoneflies (Plecoptera) of Illinois revisited. J. Kan. Entomol. Soc. 67: 340-346. Kirchner, R. F., and B. C. Kondratieff. 1997. A new species of nearctic Perlesta (Plecoptera: Perlidae) from Virginia. Proc. Entomol. Soc. Wash. 99: 290-293. Poulton, B. C., and K. W. Stewart. 1991. The stoneflies of the Ozarks and Ouachita Mountains (Plecoptera). Mem. Am. Entomol. Soc. 38: 1-116. Stark, B. P. 1989. Perlesta placida (Hagen), an eastern nearctic species complex (Plecoptera: Perlidae). Entomol. Scand. 20: 263-286. Stark, B. P., and H. A. Rhodes. 1997. Perlesta xube, a new stonefly species from Nebraska (Plecoptera: Perlidae). Entomol. News 108: 92-96. 3 1 8 ENTOMOLOGICAL NEWS TWO NEW SPECIES OF CHOREBUS (HYMENOPTERA: BRACONIDAE) FROM SPAIN1 I. Docavo , J. Tonnes-* ABSTRACT: Chorebus pseudometallicus and C. pseudoasini, two new species from Spain, are described and compared with allied species of the genus. The subfamily Alysiinae is one of the most distinctive subfamilies of the Braconidae because all members possess the exodont condition and are endoparasitoids of cyclorrhaphous Diptera. This subfamily is subdivided traditionally into two tribes: Alysiini and Dacnusini. Chorebus Haliday, whose species are endoparasitoids of Agro- myzidae and Ephydridae Diptera (there exists only one exception, a species that attacks Psila rosae [F.]), is the largest genus of Dacnusini with approxi- mately 215 Holarctic species. Many of its species are characterized morpho- logically by having a densely setose metapleuron (metapleural rosette) and usually a sculptured sternaulus. We have discovered two new species, described below, which were obtained netting on Papilionaceae, in Alcira (province of Valencia), Spain. The Dacnusini have been dealt with, both at the morphologi- cal and biological levels, by Griffiths (1964, 1966, 1968, 1984) and Tobias (1986, summary of the Palearctic taxa with keys to genera and species, trans- lated into English 1995). Terms for body morphology and wing venation follow Griffiths (1964) and Wharton( 1977, 1986). Chorebus pseudometallicus NEW SPECIES Female: Head (Figs. 1, 2, 3) - Transverse, 1.87 times wider than long, 1.25 times higher than long; occiput bare; vertex with scattered pubescence; base of the mandibles with tenuous pu- bescence; eyes in lateral view 0.87 times as long as the temples; temples bulging beyond eyes in dorsal view; eyes strongly converging below; face 1.4 times as wide as high; antennae with 23 antennomeres, apical flagellomeres ca 2.5-3.2 times as long as wide; mandibles 3-tooth, 1st relatively small, weakly expanded, blunt, 2nd tooth very long and pointed, 3rd tooth short, expanded, slightly pointed; maxillary palpi long. Mesosoma (Figs. 1, 2, 4) - 1.28 times as long as high, 1.71 times as long as width between tegulae; pronotum bare and shining, only setose along anterior oblique suture; mesonotal disc extensively bare, with only scattered setae on its anterior half and along each notaular line; midpit shallow, narrow, extending from about posterior 1/3 of disc nearly to posterior margin; notauli scarcely visible, represented by smooth fine line that seems reach midpit; sternaulus extending to posterior border of mesopleuron, narrow, shiny, practically smooth; posterior 1 Received October 21, 1997. Accepted February 25, 1998. 2 Departamento de Biologia Animal, Biologia Celular y Parasitologia. Facultad de Biologia. Universidad de Valencia. C/Dr. Moliner, 50. Burjasot (Valencia). Spain. 3 Unidad de Zoologia. Facultad de Biologia. Universidad de Salamanca. 37071 -Salamanca. Spain. ENT. NEWS 109(5): 318-324, November & December, 1998 Vol. 109, No. 5, November & December, 1998 319 1 mm 3 0.4 mm 0.4 mm 1 mm 0.4 mm 6 02 mm FIGURES 1-6. Chorebus pseudometallicus sp. nov. (female).- 1, Body (except legs and wings) in dorsal view; 2, in side view; 3, Head in lateral view; 4, Propodeum in lateral view; 5, Ante- rior right wing; 6, Petiole in dorsal view. 320 ENTOMOLOGICAL NEWS mesopleural furrow smooth; mesopleuron smooth, shiny, bare, only with a few long setae near the ventral border; metapleuron and propodeum with pubescence only moderately densely se- tose, which allows its rugose sculpture to be clearly seen; posterior coxae with a setae tuft little differentiated on its posterior margin. Wings (Fig. 5) - Pterostigma quite narrow and little darkened, 1.2 times longer than the metacarpus; 1 st radial segment shorter than the length between its insertion and the parastigma, and about as long as the pterostigma wide; remainder of radius evenly curved; n. rec. antefurcal; 3rd discoidal segment represented only by a shadow, so that cell B is open at its lower distal corner. Metasoma (Figs. 1, 2, 6) - Petiole 1.4 times longer than wide apically, glabrous, grooved, with a pronounced central ridge; ovipositor sheath setose, robust, extending slightly beyond last tergite in resting position. Color and size - Head, mesosoma and metasoma black; face black, clypeus and labrum darkish; maxillary and labial palpi dark brown - the labial palpi a little lighter-; antennae black, with yellowish brown pedicel and base of the scape; centre of mandibles reddish-yellow; mesopleuron black shiny; legs reddish-brown, with middle and posterior coxae, tibiae and tarsi more infuscated (darker); wings hyaline, with dark pterostigma. Body length: 2.1 mm. Male: unknown. Host: unknown. Material examined: [deposited in the Fundacion Entomologica "Torres-Sala" (Docavo Col- lection) (Valencia, Spain)]: Holotype: female, SPAIN: Valencia: Alcira, 30-11-1963 (leg. I. Docavo). Paratype: female, SPAIN: Valencia: Alcira, 30-11-1963 (leg. I. Docavo). Etymology: The specific name of this species makes reference to C. metallicus Griffiths [Griffiths, 1968], to which the new species is very similar. Chorebus pseudoasini NEW SPECIES This new species appears very close to C. pseudometallicus, but can be distinguished by the following characters: Female: Head - Weakly transverse, 1 .6 times wider than long, 1 .4 times higher than long; base of the mandibles with well differentiated pubescence (Fig. 7). Mesosoma - Sternaulus pointed in its anterior part; posterior coxae with a tuft of setae well differentiated on its posterior margin (Fig. 8). Wings (Fig. 9) - Pterostigma narrow, imperceptibly joining the metacarpus; cell R nar- rower and shorter, finishing before the tip of the wing; 3rd discoidal segment decolored; cell B incompletely closed. Metasoma - Petiole 2.1 times longer than wide apically, with the tubercules of the spiracles very pronounced (Fig. 10); last tergite narrow and prolonged covering the ovipositor of which only its apex can be appreciated. Color and size - anterior and middle coxae a very yellowish reddish brown; posterior very dark. Body length: 1.9 mm. Male: unknown. Host: unknown. Material examined: [deposited in the Fundacion Entomologica "Torres-Sala" (Docavo Col- lection) (Valencia, Spain)]: Holotype: female, SPAIN: Valencia: Alcira, 28-11-1960 (leg. I. Docavo). Paratypes: 2 females, Valencia: Alcira, 28-11-1960 (leg. I. Docavo). Vol. 109, No. 5, November & December, 1998 321 0.4 mm 0.4 mm 8 0.5 mm 10 ' 0.5 mm FIGURES 7-10. Chorebus pseudoasini sp. nov. (female). - 7, Mandible in lateral view; 8, Sternaulus and lateral view of the right posterior coxae; 9, Anterior right wing; 10, Petiole in dorsal view. Etymology: The specific name makes reference to the closely related C. asini Docavo [Docavo, 1 965]. Notes: These new species belong to a group which Griffiths ( 1 964) has described as the "affinis complex". They are similar to C. metallicus and C. asini, from which they differ in the following respects: a) number of antennomeres; b) pubescence from the base of the mandibles, sides of pronotum, mesonotal disc, metapleuron (rosette), posterior coxae (setae tuft) and propodeum; c) coloring of the legs (particularly of the coxae); d) morphology of the pterostigma, cell B (bra- chial cell), sternaulus and metasoma (specially petiole morphology); e) body length. 322 ENTOMOLOGICAL NEWS C. pseudometallicus can be differentiated from: C. metallicus: a) By the clearly differentiated metapleuron pubescence (ro- sette) (Figs. 2, 4) [less differentiated in C. metallicus]; b) coxae reddish-brown [in C. metallicus gold yellow]; c) anterior oblique suture of the pronotum pu- bescent (Fig. 2) [in C. metallicus the sides of the pronotum are bare and shiny]; d) mesonotal disc slightly pubescent (Fig. 1 ) [C. metallicus has only a few setae along the notauli]; e) pubescence on propodeum more dense than in C. metallicus (Figs. 1, 2, 4). C. asini: a) Antennae with 23 antennomeres [C. as//?/ has 25 antennomeres]; b) mesonotal disc more shiny and less pubescent (Fig. 1 ) [in C. asini dull and much more setose]; c) legs lighter; d) sides of pronotum pubescent (Fig. 2) [in C. asini they are practically glabrous]; e) sternaulus narrow, pointed anteriorly (Fig. 2) [in C. asini completely smooth]; f) pterostigma narrower (Fig. 5). C. pseudoasini: Due to the morphological characters previously explained in the description of this species. The differences between this species and C. asini are the same as those of C. pseudometallicus except the number of antennomeres which is 23. The most important characteristic for recognizing these species lie in well differentiated metapleural rosette for C. pseudometallicus (Figs. 2, 4), and in well differentiated setae tuft in the posterior coxae for C. pseudoasini (Fig. 8). The four species: C. pseudometallicus, C. metallicus, C. pseudoasini and C. asini, can be distinguished in table I. Table I - Morphological characterization of C. pseudometallicus, C. metallicus, C. pseudoasini and C. asini [differentiated character (*); scarcely differentiated or missing (-)]. Number of antennomeres (1). Pubescence well differentiated in: base of mandibles (2), sides of pronotum (3), mesonotal disc (4), metapleuron (rosette) (5), propodeum (6); coxae dark (7), pterostigma narrow (8); cell B completely open (9); sternaulus completely smooth (10); posterior coxae (setae tuft) (11), Tubercles of the spiracles greatly differentiated (12). Species 1 2 3 4 5 6 7 8 9 10 11 12 C. pseudometallicus 23 * ***.*_... C. metallicus 21/22 - .-*-*-- C. asini 25 * ***__*__ C. pseudoasini 23 * * ***_ **_ * # Vol. 109, No. 5, November & December, 1998 323 These species can be inserted in the keys of Tobias (1995: 340) as follows: Females (475) (476) (475a) (475b) Antennae with 23 antennomeres. Mesonotal disc more or less shiny, with a few setae along the notauli and central anterior part. Pronotum shiny, with a few setae, fine and scattered, along the anterior oblique suture. Petiole 1.6 to 2.1 times longer than wide, shiny, practically bare, with a well differentiated central ridge and longitudinal grooving. Propodeum scarcely pubes- cent, more differentiated at its sides, although allowing the sculpture situated underneath to be perceived. Pubescence of the metapleuron (rosette) and propodeum well differentiated and characteristic of the genus Chorebus. (475b) (475c) Anterior coxae reddish brown, middle and posterior a darker brown with reddish over- tones. Petiole almost bare, grooved, with a well differentiated central ridge. Metasoma oval, elongated, less rounded on the apex than in C. pseudoasini, without the last tergite being elon- gated and narrow; ovipositor, therefore, more protruding than in mentioned species. Pterostigma wider than in C. pseudoasini, not so imperceptibly joining the metacarpus. Cell B somewhat open in its lower distal corner, with just a trace of the 3rd discoidal segment. Length of body: 2 mm. Host: unknown. C. pseudometallicus sp. nov. (475c)(475) Anterior and middle coxae, a more yellowish reddish brown, but the posterior, partly, darker. Petiole shorter, but with extremely protruding stigmatipherous tubercules. Metasoma rounder, with the last tergite narrow and prolonged, covering the ovopositor, of which only the apex can be seen from above, while in lateral view the ovopositor appears curved upwards. Pterostigma narrow, imperceptibly joining the metacarpus. Cell R narrower and shorter, finish- ing beyond the extremity of the wing. Cell B completely open, as 3rd discoidal segment is completely missing. Length of body: 1 .9 mm. Host: unknown. C. pseudoasini sp. nov. (475) (476) ACKNOWLEDGMENTS We are much indebted to Cees van Achterberg (Nationaal Natuurhistorisch Museum, The Netherlands) and Max Fischer (Naturhistorisches Museum Wien, Osterreich), for their com- ments on the manuscript. Financial support for this paper was provided from the Junta de Castilla y Leon, project SA 18/96. LITERATURE CITED Docavo, I. 1965. Nuevas aportaciones al conocimiento de los Dacnusini de Espana (Hym., Braconidae). Graellsia, XXI: 25-39. Griffiths, G.C.D. 1964. The Alysiinae (Hym., Braconidae) parasites of the Agromyzidae (Diptera). I. General questions of taxonomy, biology and evolution. Beitr. Entomol., 1 4: 823-914. Griffiths, G.C.D. 1966. The Alysiinae (Hym., Braconidae) parasites of the Agromyzidae (Diptera). II. The parasites of Agromyza Fallen. Beitr. Entomol., 16: 551-605. 324 ENTOMOLOGICAL NEWS Griffiths, G.C.D. 1968. The Alysiinae (Hym., Braconidae) parasites of the Agromyzidae (Diptera). VII. The parasites of Cerodontha Rondani s.l. Beitr. Entomol., 18: 63-152. Griffiths, G.C.D. 1984. The Alysiinae (Hym., Braconidae) parasites of the Agromyzidae (Diptera). VII. Supplement. Beitr. Entomol., 34: 343-362. Tobias, W.I. 1986. Identification key for the insects of the European part of the URSS. Vol. III. Part V. Hymenoptera, Braconidae. pp. 100- 105 (key for genera of Alysiinae), 163-221 (Dacnusini). Akademia Nauk: Leningrad (in Russian, transl. 1995 in English). Wharton, R.A. 1977. New World Aphaerela species (Hymenoptera: Braconidae) with a dis- cussion of terminology used in the tribe Alysiini. Ann. Ent. Soc. Am., 70: 782-803. Wharton, R.A. 1986. The braconid genus Alysia (Hymenoptera): a description of the subgen- era and a revision of the subgenus Alysia. Syst. Ent., 1 1 : 453-504. BOOKS RECEIVED AND BRIEFLY NOTED INVERTEBRATE SURVEYS FOR CONSERVATION. T.R. New. 1998. Oxford Univ. Press. 240 pp. $35.00 (paperback). A comprehensive guide to the ecological methods used to survey invertebrate animals in ter- restrial, freshwater, and marine environments. It describes how to select taxonomic groups for study, how to collect and analyse samples, and how to set priorities for protection. MELANISM. EVOLUTION IN ACTION. M.E.N. Majerus. 1998. Oxford Univ. Press. 338 pp. $105.00 (cloth); $45.00 (paperback). Placing melanism into its historical and scientific context, the author considers the diversity of melanism in the animal and plant worlds, and its physical and genetic properties. Examining mela- nism in moths and ladybeetles in detail, he explores the diversity of evolutionary reasons for mela- nism and the complexities underlying this phenomenon. THE BIRDER'S BUG BOOK. G. Waldbauer. 1998. Harvard Univ. Press. 290 pp. $27.95 (hardcover). This book is an interesting introduction into the many fascinating relationships between birds and insects. As past eons have come and gone, birds and insects have become increasingly en- meshed in a complex web of interrelationships: birds eating insects, bloodsucking insects feeding on birds, parasitic insects infesting birds, and birds struggling to rid themselves of the parasites. In this book, the author describes these and many other interactions between birds and insects. Vol. 109, No. 5, November & December, 1998 325 DIPTEROUS PARASITOIDS FROM ADULTS OF MOTHS (LEPIDOPTERA)1 Timothy L. McCabe2 ABSTRACT: An adult moth, Cucullia lucifuga (Lepidoptera: Noctuidae), was parasitized by the maggot of Sarcophaga aldrichi (Diptera: Sarcophagidae). Circumstances suggest that the fly, which is a scavenger and a larval and pupal parasitoid of Lepidoptera, also attacks the adults of moths. This is the first report of a sarcophagid fly from the adult of a moth. The maggot of a phorid fly, Megaselia rufipes, is reported from the adult of another noctuid moth, Amphipyra glabella. It is unusual to have the adult stage of a moth or butterfly parasitized. Adult lepidopterans with dipterous parasitoids of the families Tachinidae and Phoridae are known. Tachinid observations were presumed to be examples of larval or pupal parasitoids that carried over into the adult (Cockayne, 1911; De Vries, 1 979; Smith, 1 98 1 ). Flemyng (1918) reported an adult sphingid para- sitized by a phorid fly, Megaselia rufipes (Meigen). Flemyng 's original obser- vation was too casual to ascertain whether the living moth or the carcass had been attacked. Borgmeier (1965) considers M. rufipes maggots to be polypha- gous; Robinson (1971) documents a wide range of hosts from lepidopteran pupae to bat guano. Flemying's report, however, may have some credence. In July of 1997, I held a captive female Amphipyra glabella (Morrison) (Lepidoptera: Noctuidae), collected near Albany, New York, in a tightly sealed container for a week. Soon after the moth's death maggots could be observed. Two female flies emerged after ten days. These proved to be M. rufipes. De- spite frequently having held moths for eggs, this was my first observation of phorid parasitoids killing a moth. Whole abdomens of moths are treated chemi- cally when lepidopterists prepare specimens for dissection, and I have only twice encountered unidentified phorid maggot exoskeletons in the course of three thousand dissections. The following represents the first report of a sarcophagid parasitoid of an adult lepidopteran. Sarcophaga aldrichi Parker is an important internal parasitoid of the pupa of tent caterpillars. Hodson (1939) proved unequivocally that tent caterpillar larvae were not attacked, but that the pupae were. The fly has been reported as a parasitoid of the larva of the satin moth (Lejeune and Silver, 1961) and the gypsy moth (Hodson, 1939). Gypsy moth, satin moth, and tent caterpillars all make an exposed cocoon. Sarcophaga aldrichi is viviparous. A female lays its first instar maggot on the silk of the host's cocoon. The maggot finds its way through the silk to the pupa and feeds internally for 3 to 5 days. After this 1 Received January 22, 1 998. Accepted March 13, 1998. 2 Biological Survey, New York State Museum, Albany, New York 12230. ENT. NEWS 109(5): 325-328, November & December, 1998 326 ENTOMOLOGICAL NEWS period the maggot may stay within the pupal cadaver for more than a month, eventually dropping to the ground to pupate (Hodson, 1939). Sarcophaga aldrichi also will breed in decaying organic material and carrion (Hodson, 1939) as do most Sarcophaga species (Sanjean, 1957). On July 22, 1987, an adult female of Cucullia lucifuga [Denis and Schiffermiiller] was captured nectaring at milkweed blossoms on the pine bar- rens near Albany, New York. The moth was held for oviposition in a plastic cup with a tight fitting lid. The moth died without ovipositing on July 24th. The following day the moth carcass had the legs, wings, and head detached. No internal organs remained. A third (ultimate) instar Sarcophaga (Diptera: Sarcophagidae) maggot was present. It had been an internal parasitoid of the moth. The maggot was offered additional fresh, decapitated, live moths and fresh cadavers of moths, but refused to feed. The maggot was kept in a moist container. It lived 51 days, until September, but died. Its carcass was preserved and identified as Sarcophaga aldrichi. The caterpillar of C. lucifuga is a flower feeder. When fully grown, the caterpillar moves to the ground and enters the soil. It pupates in an under- ground cocoon. My pupation boxes have 15 centimeters of peat and C. lucifuga typically pupates at the bottom. The species is triple brooded in the north and the summer brood has a pupal stage that lasts for 10 days or more. Therefore, the sarcophagid loses its opportunity to parasitize C. lucifuga once the cater- pillar enters the soil. The fly is probably not a pupal parasitoid here, although it would have had the opportunity to parasitize the larva. Hodson (1939) did not observe larval parasitism by S. aldrichi in 100 field collected last-instar larvae of tent caterpillars. Sixty percent of the tent caterpillar pupae from Hodson 's experi- mental site had been parasitized by 5. aldrichi. The sarcophagid maggot completes its development in 3 to 5 days (Hodson, 1939). Sarcophaga aldrichi is a large fly, larger than a house fly, and there is no chance of a parasitized host the size of C. lucifuga surviving. A large satyrine caterpillar has been reported as surviving despite having been parasitized by a tachinid maggot (DeVries, 1984); the caterpillar survived the exiting of the maggot and ultimately produced an adult. The maggot was not carried over to the butterfly's adult molt. In the present case the adult may have been the stage initially parasitized. The reasoning for this is as follows: 1 ) I estimate the moth was 4 -7 days of age at death (age determination based on my observations of many captive and bred lepidopterans); 2) maggot development time is 3-5 days; 3) the maggot was large enough to kill an organism the size of C. lucifuga at any stage; 4) the pupa was probably not parasitized because pupation takes place deep under- ground; 5) the larva was probably not parasitized because 5. aldrichi seldom attacks larvae and the maggot development time of 3-5 days is too quick; 6) Vol. 109, No. 5, November & December, 1998 327 the moth is active at early dusk, while there is still light, and both fly and moth can be found visiting milkweed blossoms simultaneously. Given the relatively constant development time for the maggot, one would expect a small to mid-size imago to be killed within 3 to 5 days of attack. When I collected the moth, which I determined to be mated based on clasper marks, I estimated it to be 2-5 days of age. When the parasitoid emerged the moth would have been 4-7 days of age, sufficient time for a first stadium mag- got to have entered the imago and completely matured. The preserved maggot agrees well with the description and illustration of 5. aldrichi given in Greene (1925). The maggot has anterior spiracles with multiple rows of lobes. This is a rare condition in Sarcophaga. The lack of spines around the posterior pocket and the prominent anal tubercles are all concordant with S. aldrichi morphology. The mouthparts of the preserved 5. aldrichi maggot are illustrated (Fig. 1). Mouthparts were not illustrated in Greene's (1925) paper and prove to be valuable for species recognition. Sub- sequent captures of adult Cucullia in the last ten years have not rediscovered the parasitoid and it may prove to be a rare event. Fig. 1 . Sarcophaga aldrichi. Cephalo-pharyngeal skeleton, showing mouthhooks and associated structures, third instar. Scale line = 0.25 mm. ACKNOWLEDGMENTS I thank Christine Weber and Charles Sheviak for reviewing the paper and for helpful com- ments. Specimens are vouchered in the New York State Museum. This is published as Contri- bution number 738 of the New York State Science Service. LITERATURE CITED Cockayne, G. A. 191 1. A dipterous parasite bred from the imago of Nyxsia lapponaria. Ento- mologist 44: 253. 328 ENTOMOLOGICAL NEWS Borgmeier, T. 1965. Revision of the North American Phorid flies. Part III. The species of the genus Megaselia, subgenus Megaselia (Diptera: Phoridae). Stud. Entomol. 8: 1-160. DeVries, P. J. 1979. Occurrence of fly maggots in adult Morpho theseus (Lep.: Morphinae) females from Costa Rica. Brenesia 16: 223. DeVries, P. J. 1984. Butterflies and Tachinidae: does the parasite always kill its host? J. Nat. Hist. 18: 323-326. Flemyng, W. W. 1918. Sphinx convolvuli attacked by larvae of Diptera. Irish Nat. 27: 13. Greene, C.T. 1925. The puparia and larvae of sarchophagid flies. Proc. U. S. Natl. Mus., Vol. 66, Art. 29: 1-26. Hodson, A. C. 1939. Sarcophaga aldrichi Parker as a parasite of Malacosoma disstria Hbn. J. Econ. Entomol. 32(3): 396-401 Lejeune, R. R. and G. T. Silver. 1961 . Parasites and hyperparasites of the Satin Moth, Stilpnotia salicis Linnaeus, (Lymantriidae) in British Columbia. Can. Entomol. 93: 456-467. Robinson, W. H. 1971. Old and new biologies of Megaselia species (Diptera, Phoridae). Stud. Entomol. 14:321-348. Sanjean, J. 1957. Taxonomic studies of Sarcophaga larvae of New York with notes on the adults. Cornell Univ. Agric. Exp. Stat. Mem. 349: 1-115. Smith, K. G. V. 1981. Atachinid (Diptera) larva in the abdomen of an adult moth (Geometridae). Entomol. Gaz. 32: 174-176. Vol. 109, No. 5, November & December, 1998 329 BIOLOGY OF TANYCHELA PILOSA (HYMEMOPTERA: ICHNEUMONIDAE), A PARASITOID OF THE AQUATIC MOTH PETROPH1LA CONFUSALIS (LEPIDOPTERA: PYRALIDAE)1 Wanda Jamieson^, Vincent H. ABSTRACT: Tanychela pilosa, a solitary endoparasite of the aquatic moth Petrophila confusalis, has hymenopteriform eggs that are oviposited in late summer or early fall, in the Clearwater River drainage of western Montana, USA. The first instar is a caudate-mandibulate type. Su- perparasitism may result from polyembryony but only a single wasp larva survives. Larvae undergo heteromorphosis as a third and final instar. Pupation occurs in the dry cocoon of the moth. Adult sex ratios approach 1:1. 72.2% of sites examined in the Clearwater River drainage had P. confusalis and 61.5% of these contained T. pilosa. Emergent rocks may enable oviposit- ing females to enter streams and search for caterpillars. Parasitization of P. confusalis reported from Idaho, Washington, and California may be by T. pilosa. Hymenoptera parasitizing aquatic insects are rarely encountered in collec- tions of stream insects. In an earlier study, we discovered that a population of the aquatic lepidopteran Petrophila confusalis (Walker) was parasitized by an aquatic wasp (Resh and Jamieson 1988) in Owl Creek, Missoula County, Montana (Fig. 1). The rate of parasitism in the moth population ranged from 55% at the outlet of Placid Lake into Owl Creek to 0% less than 5 km down- stream from the lake outlet. Wasp pupae were identified as Tanychela pilosa Dasch, the type specimen of which is from Mexico (Dasch 1979). In our original study, we indicated that parasitism occurred during the pu- pal stage of the aquatic moth, as has been reported for the agriotypid (now generally considered a subfamily of the Ichneumonidae) wasp Agriotypus armatus Curtis that parasitizes the caddisfly Silo pallipes (Fabricius) in Eu- rope (Elliott 1982). In retrospect, if this supposition were correct we would have expected to see some type of activity indicating that moth pupae were being parasitized, such as adult wasps near the pupal patches that occurred on rocks in the streambed, or wasp eggs or larvae inside the moth pupae or moth cocoons. Because of the lack of detailed information on hymenopteran parasites of aquatic insects (Hagen 1996), the present study was initiated to (1) describe the biology of the parasitoid T. pilosa and (2) document the distribution of T. pilosa beyond Owl Creek through surveys conducted in the Clearwater River drainage system of western Montana. 1 Received October 11, 1997. Accepted January 12, 1998. ^ z Rathead Lake Biological Station, University of Montana, 31 1 Biostation Lane, Poison Mon- tana 59860. * Division of Insect Biology, 201 Wellman Hall, University of California, Berkeley, California 94720-31 12. Please send reprint requests to this author. ENT NEWS 109(5): 329-338, November & December, 1998 330 ENTOMOLOGICAL NEWS Fig. 1 . Petrophila confusalis and its endoparasite Tanychela pilosa. METHODS AND MATERIALS In May 1989, lepidopteran larvae were collected from Owl Creek near the outlet of Placid Lake (Fig. 2). During the summers of 1993, 1994 and 1995, samples of approximately 100 P. confusalis larvae and pupae were collected randomly from various sites along the Clearwater River and its tributaries (Fig. 2). Head widths of the moth larvae and prepupae were measured to determine instar classification; the caterpillars were then dissected to determine rates of parasitism. Wasp cocoons were dissected to determine stage of development and sex of the wasps. Life cycle observations were based on examinations of more than 1 400 aquatic wasp larvae and pupae, the dissections of more than 1900 aquatic moth larvae and prepupae, and the examination of more than 2000 empty wasp and moth cocoons. BIOLOGY OF TANYCHELA PILOSA Egg stage: T. pilosa wasps are solitary endoparasites of P. confusalis cat- erpillars. The biology of the host (sometimes referred to as Paragyractis confusalis) is described by Tuskes ( 1 977, 1 98 1 ), Me Auliffe & Williams ( 1 983), Bergey (1995), and summarized by Lange (1996). In late summer or early fall, the female wasp deposits an egg within the abdominal cavity of the moth larva. The pale yellow, jellybean-shaped eggs, about 0.5 mm long and 0.2 mm wide, are the hymenopteriform type (Hagen 1964, Fig. 14a). Larval stage: Parasitism could occur during the moth's free-living first instar or during its other larval stages that are spent beneath silken retreats that Vol. 109, No. 5, November & December, 1998 331 Swan R. Swan Rs«' Cygnet L. 'Clearwater L. I Clearwater R. Fig. 2. Sampling locations in the Clearwater Drainage, western Montana, USA. 332 ENTOMOLOGICAL NEWS provide shelter (Lange 1996, Fig. 19.1). The free living, first-instar caterpillar is more vulnerable to predation and to dislocation (i.e., drift), and the duration of this stage is brief, e.g. first instars of P. confusalis occur in Owl Creek (Fig. 2) during a brief two-week period (McAuliffe & Williams 1983). In contrast, second, third, and fourth instars occur from mid-August to mid-October (McAuliffe & Williams 1983), and the female wasp could penetrate the silken covering of these instars with her ovipositor, laying her eggs within the host caterpillar. The newly hatched wasp larva is less than 2 mm long. This primary or first instar is of the caudate-mandibulate larval type described by Hagen (1964, Fig. 18a, b, c). It has a brownish sclerotized head capsule with a black man- dible. Its long, slender, segmented body is enclosed in a colorless, transparent integument; the internal body mass is bright yellow. A long "tail" that is al- most equal in length to the rest of its body extends posteriorly from the dorsal portion of the wasp larva. Ullyett (1944) suggests the long tail may serve as one and/or all of the three following functions: an egg burster, a balancing organ to compensate for its large head, or for food absorption. Although the first instar wasp has mandibles, we found no evidence in any of the host larvae that internal tissues or organs had been damaged, nor did we ever observe any tissue attached to the mandibles of the wasp larva that would indicate it had been feeding. Apparently, the wasp larva absorbs food through its very thin cuticle from its host's hemolymph. Strands of fat bodies were noticeably re- duced or absent in the hemocoels of parasitized caterpillars. Because there are no spiracles on the first instar larva, respiration is probably cutaneous. T. pilosa overwinters in the first instar within its host. The wasp larva grows very slowly from October to June and growth appears to be relatively constant from year to year. For example, wasp larvae in samples collected from Owl Creek and the Lake Inez outlet (Fig. 2) on 23 Oct 1993 were very similar in size to the wasp larvae in samples collected during June 1994 and June 1995 from the same streams. In areas where the rate of parasitism is high, two or more parasitoids may occur in one host (i.e., superparasitism). For example, of 44 moth larvae that were collected 23 Oct 1993 from Lake Inez (Fig. 2), 32 were parasitized. Of these, six (19%) caterpillars had been parasitized more than once; three of them had two larval parasites and each of the other three had three, four, and five parasitic larvae, respectively. Several genera of Ichneumonidae that parasitize the eggs and larvae of Lepidoptera exhibit polyembryony (Hagen 1964, Chapman 1982) and this could account for the multiple T. pilosa parasitoids within the host P. confusalis cat- erpillar. We have not determined how long the different larvae within a single host live, but we have observed that eventually all but one die. For example, in a sample taken 15 Aug 1995 from the Lake Inez outlet (Fig. 2), one late-instar moth larva had 10 wasp larvae within its hemocoel. All wasp larvae were the same size, and had sclerotized heads and transparent body coverings, but only Vol. 109, No. 5, November & December, 1998 333 one was wiggling; apparently nine of the wasp larvae had just died. Thus, this moth larva had been host to 10 wasp larvae for 9 -10 months. The dead wasp larvae can be found in the last abdominal segment; per- haps, the wiggling motion of the caterpillar forces these dead larvae to the posterior end of the moth's abdominal cavity where they remain (and subse- quently can be counted). We noted that when host caterpillars contained only one wasp larva, that larva was usually alive. The bodies of the dead wasp lar- vae become white and opaque; their sclerotized head capsules enabled us to distinguish them from the fatty tissues of the caterpillar. Hagen ( 1 964) reported that the number of larval instars among hymenopter- ous parasites is variable. However, there appears to be a tendency for ecto- parasitic larvae to have five instars and endoparasitic forms to have fewer than five (and often three). During the fifth instar of the caterpillar, the wasp larva grows rapidly and undergoes heteromorphosis (sensu Chapman 1982; Hagen 1964 refers to this as hypermetamorphosis). The sclerotized head capsule splits along the dorsal line and the wasp larva emerges from its exuvium with a new body form. The new segmented body is covered with a loose transparent membrane. The body internally is bright yellow. During this metamorphosis, the wasp larva abruptly changes from a slender 2-mm long caudate-mandibulate type larva to a fat, globular, grub-like larva that eventually grows to fill the thorax and abdomen of the 10-mm long caterpillar within four weeks. This grub-like larva may represent the third instar. We examined the head-capsule width of approximately 100 caterpillars from sites D in 1993, and N in 1993 and 1995 (Fig. 2). No size-class differ- ences were observed in parasitized and unparasitized caterpillars. Pupal stage: Prior to its pupation, the P. confusalis caterpillar modifies its silken retreat on a rock surface to form a thicker, oval patch. This patch is referred to as the external pupal case in Resh and Jamieson (1988, Fig. 1 ). The patch has semicircular openings at the edge [as described by Lloyd 1 9 1 4, 1 9 1 9 for Petrophilafulicalis (Schaus)] and the caterpillar scores a C-shaped slit at the upstream end of the patch. Beneath the patch, the caterpillar spins a water- proof cocoon that provides a dry environment for the pupal stage. When it has completely enclosed itself within a cocoon, the caterpillar becomes an immo- bile prepupa. When examined externally, there is no indication whether or not the caterpillar prepupa is host to a parasite. If parasitization has been successful (i.e. sometimes the wasp larva dies before heteromorphosis occurs), the wasp larva emerges from its host during the caterpillar's prepupal stage. During emergence, the thoracic and abdomi- nal skin of the caterpillar appears to disintegrate within the cocoon. The head sclerites and other tissues of the moth fall to the posterior end of the cocoon as the wasp larva emerges from its host within the dry environment of the host's cocoon. At this time, spiracles are very prominent on the wasp larva. The wasp larva immediately begins to spin its own cocoon within the 334 ENTOMOLOGICAL NEWS caterpillar's cocoon and the wasp's cocoon is completed within a few hours. Of the 5,000+ specimens examined for this study, <0. 1 % had wasp larvae that were outside the host but not yet enclosed in their own pupal cocoons. The structure of the wasp cocoon indicates that it is spun in a circular (or sideways) manner back and forth from one end of the cocoon to the other, until it is about three layers thick. During this process the wasp larva is visible be- tween the strands of the cocoon. When the cocoon is completed, the wasp larva is no longer visible. The cocoon is dark brown, leathery, oval-shaped, and about 8 mm long. When the cocoon is turned inside out and placed under- water, it takes on the metallic sheen characteristic of an unwettable surface. During the construction of the cocoon, the wasp larva alternately faces anteri- orly and posteriorly within the cocoon. But in the final phase of its prepupal stage, the wasp larva always faces anteriorly within the cocoon, i.e., it points its head in the same upstream direction that the caterpillar would have pointed its head. Because the stage of development cannot be determined until the cocoon is dissected, all specimens within wasp cocoons have been described as pupae. However, within this category, we have identified four phases of development from what we found when the anterior end of the cocoon was opened. First, in the "early" phase, which is actually still part of the larval stage, the larva is still mobile within the cocoon and responds to touch when the cocoon is opened. Morris (1937) referred to this as the eonymphal phase. As development pro- ceeds, the wasp larva begins to transform into the pupal form. The colorless, transparent membranous skin becomes white and opaque. Another change in the larva is the formation of red spots where the compound eyes will form. Differentiation of body regions begins. Morris (1937) referred to this final, immobile, phase as the pronymphal stage. During the second (or white) phase, three distinct body regions have formed; the head is white and the compound eyes are red. Long legs and long antennae have formed and in females the ovipositor is very well developed. In the later part of this phase, although the head is still white, the thorax becomes black, the eyes dark brown, and wing pads start to form. The third (or black) phase is characterized by the pupa having a black head, black antennae, black thorax, black legs, a brown and white striped ab- domen, and very prominent wing pads. Both the white phase and the black phase are enclosed within a transparent membrane. A meconium is attached posteriorly to the developing pupa (Resh and Jamieson 1988, Fig. 10). These three phases each require about one week. During the fourth (or pre-adult) phase, the wasp pupa has shed the transparent membrane within the cocoon, the meconium has become detached, and the wings are fully expanded. Adult stage: To emerge from its cocoon, the adult wasp chews a hole dor- sally through its cocoon and through the lepidopteran's external pupal case; it does not use the C-slit made by the caterpillar for its own emergence. When the newly emerged wasp comes in contact with the water, an air bubble from Vol. 109, No. 5, November & December, 1998 335 the cocoon surrounds its hairy body. We observed that when an adult female wasp emerged underwater (e.g. in a dish that contained moss and pebbles; we have not observed this in nature), she grasped a sprig of moss with her hind leg and groped in the dish with her other five legs. In another cocoon that was opened underwater, we observed that after a female emerged from the water she gripped the wooden handle of the teasing needle; then, with her hind legs, she wiped down her abdomen and ovipositor and, with her front legs, wiped her head and antennae. A few minutes after drying off, she flew away. Based on the observations of 575 wasp pupae, the sex ratio of the wasps approaches 1 : 1 (290 females: 285 males). Some streams, however, had collec- tions favoring females (e.g. Fig. 2, site B, 43:11) or males (e.g. Fig. 2, site F, 8:22; site L, 7:22). Emergence of wasps begins about two weeks after the unparasitized moths have emerged. Once the moths have emerged from their cocoons, decomposi- tion of their pupal patches begins and lasts for approximately one additional month. The extended duration of the lepidopteran silken patch until the wasps emerge is crucial to the wasps' existence and survival. Parasitization of the moth larvae varies even on a single rock. For ex- ample, 18 lepidopteran patches were collected 23 Jul 1995 from one rock at the mouth of the Clearwater River (Fig. 2, site I). Among these patches: five contained cocoons from which moths had emerged; three held moth larvae, one of which was decomposing and another was parasitized; three were moth pupae, one in an early stage of pupal development and the other two in a later stage with scaled wings; seven specimens were wasp pupae, with three in the early phase, three pre-adults (one male and two females), and one in a dam- aged condition. DISTRIBUTION AND PARASITISM Of 18 sites sampled in the Clearwater River Drainage, P. confusalis was found at 1 3 sites: B, C, D, F, G, H, I, J, K, L, M, N and Q (Fig. 2). T. pilosa parasitized P. confusalis at eight of those sites: B (55% parasitism 29 Jul 1987, 44% 26 Jun 1988, 5% 3 Jul 1988, 41% 10 Jul 1988, 36% 22 Jul 1993, 45% 14 Jul 1993, 44% 28 Jul 1993, 50% 3 Aug 1993, 35% 11 Aug 1993, 13% 23 Oct 1993, 13% 25 Jul 1994, 29% 5 Jul 1995); C (24% 9 Aug 1987, 5% 26 Jun 1988,4% 16 Jul 1993); F (58% 22 Jul 1993, 43% 27 Jul 1994); G (41% 28 Jul 1993, 41% 3 Aug 1993, 48% 27 Jul 1994, 47% 23 Jul 1995, 74% 15 Aug 1995, 80% 22 Oct 1995); H (29% 3 Aug 1993, 20% 27 Jul 1994, 50% 5 Jul 1995); I (73% 3 Aug 1993, 64% 27 Jul 1994, 54% 23 Jul 1995); L (35% 22 Jul 1993, 46% 25 Jul 1994); N (75% 22 Jul 1993, 71% 11 Aug 1993, 67% 23 Oct 1993, 48% 12 Jul 1994, 47% 19 Jun 1995, 57% 15 Aug 1995). Variations in rates of parasitism at individual sites may have been caused by sampling of different microhabitats at a site. What were the characteristics of the sites where either P. confusalis or T. pilosa were absent? No lepidopterans were found in the cold water (10°C) of the West Fork of the Clearwater River on 28 Jul 1993. Algal patches on the rocks of the warm Marshall Lake outflow (Fig. 2, site O) suggested a lepi- dopteran population may have been present earlier in the season but had 336 ENTOMOLOGICAL NEWS emerged. The Cleanvater River at Highway 83 (R) was sampled on 2 Aug 1993; the water was very cold (11°C) and no moths were found. On 3 Aug 1 993 the Blackfoot River at the Russell Gates Fishing Access (J) was sampled. The river was wide, shallow, and swift, and the rocks in the streambed were large, round, smooth and very slippery. The lepidopteran population was very sparse and only 1 1 specimens could be collected; none were parasitized. The Blackfoot River at the Roundup Fishing Access (K) also had no parasitism in the 49 moths examined. The river here was very deep, wide, and swift. Sam- pling was limited to a rocky area near the shore. Samples outside the Clearwater Drainage were also collected during sum- mer 1993. No parasitism was found in a sample taken on 26 Jul 1993 from the outflow of Loon Lake on the Fisher River in Lincoln County, Montana, nor in a sample of 145 specimens taken on 27 Jul 1993 from the Lower Crow Creek above the Lower Crow Reservoir near Pablo in Lake County. Although moth pupae and larvae were abundant in Lower Crow Creek, the water was deep and swift. Ashley Creek in Flathead County was sampled 30 Jul 1993 at its outflow from Ashley Lake and near the bridge between Lake Monroe and Lone Lake; no parasitism of the moth larvae or pupae present at either site was evident. The patches were extremely calcified and this may have prevented wasps from chewing their way out of their cocoons even if parasitism had occurred. Three sites (S, T, U) in the Swan River drainage system adjacent to the headwaters of the Clearwater River (Fig. 2) sampled on 2 Aug 1 993 did not have lepidopteran populations. Algae mats were thick on rocks in the streambed and the water was very slow moving in Holland Lake Creek (T). Although the water was warm at the Lake Lindbergh outlet (U), it was very deep and swift. At site S, the Swan River was deep and wide. There was no parasitism in a sample of 65 specimens collected from Swan River below the dam near Bigfork on 10 Aug 1993. The water there was also deep and very swift. FACTORS AFFECTING T. PILOSA DISTRIBUTION From the above information, it is apparent that parasitization by T. pilosa does not occur in all streams that had P. confusalis populations. Reasons un- derlying this distribution are not readily evident. For example, Lake Inez and Lake Alva (Fig. 2, sites N and Q) are two very similar lakes in the Clearwater River system, with Lake Alva just to the north of Lake Inez in the upper por- tion of the drainage. Both are warm-water lakes and are surrounded by sum- mer homes. Wooden plank structures dam the outlets of each lake, and the outflow streams have rocky bottoms and banks lined with vegetation. Each stream supports a population of P. confusalis. However, parasitization at the Lake Alva outflow is 0% while parasitization at the Lake Inez outflow is 75%. Why is there such a difference in parasitism when these lake outlets are less than 5 km apart? Water in the Lake Alva outlet flows smoothly (i.e. no Vol. 109, No. 5, November & December, 1 998 337 emergent rocks or riffles) over a streambed of uniformly sized rocks that are densely covered with lepidopteran pupal patches. When the water level goes down in late summer, the stream still flows smoothly. However, at Lake Inez, the outflow stream does not have a streambed of uniform composition and the water does not flow smoothly; instead, riffles form from emergent rocks and boulders interspersed among sand and gravel bars. Lepidopteran patches can be found on the large rocks and boulders as well as on small rocks and pebbles. In late summer, the larger rocks project above the water; if the female wasp requires a landing place before she enters the water for oviposition, then the Lake Inez outflow would be a possible habitat whereas Lake Alva would not. Given that the above-described habitats differ in terms of emergent rocks or riffles, and that this was often a feature lacking in sites where moths occurred but parasitism was absent, we suggest that this may be a key feature in deter- mining the local distribution of T. pilosa. Perhaps the wasp adult requires emerg- ing rocks to enter the water and remain attached while it searches for a poten- tial host. Our laboratory observations of adults holding on to objects suggest that this is the case. Owl Creek has characteristics of both Lake Inez and Lake Alva outflows. Riffle regions alternate with pool regions. No parasitism was found in 5 1 speci- mens collected 23 Jul 1995 at Owl Creek (Fig. 2, site B) from a pool area located behind a line of rocks across the stream. However, in a riffle area at this site, 22 of the 57 specimens collected were parasitized. The rocks in this riffle area project above water in late summer. Gustin (personal communication) found a species of Tanychela parasitiz- ing populations of P. confusalis in the Potlatch River and several of its tribu- taries in Latah Co, Idaho and in the Palouse River system in Latah County, Idaho, and Whitman County, Washington. The immature ichneumonids found in his study and the unnamed immatures previously reported by Tuskes ( 1 977) in California may also be T. pilosa. If the distribution of T. pilosa follows that of P. confusalis, it would include central California, north to British Columbia and east to Nevada, Idaho, and Montana (Monroe 1972). However, because the type specimen of T. pilosa is from Mexico, other species of moths may also be parasitized. Local factors may also affect distribution. Cool summers may favor the growth and development of the wasp larvae whereas warm summer tempera- tures may favor the rapid growth of fifth instar caterpillars and, consequently, earlier pupation and emergence. If T. pilosa cannot follow the rapid transfor- mation of its host, then it will not be ready for its own pupation. Because the larval wasp occurs within the caterpillar's body and the moth occurs within an air-filled cocoon (Lloyd 1919), the only aquatic portions of T. pilosa s life cycle are when the wasp adult emerges underwater and then returns to water to lay its eggs. This life history fits even the most narrow definition of an aquatic insect (Hagen 1996). However, like many other para- 338 ENTOMOLOGICAL NEWS sitic insects that occur in aquatic environments, once inside its host the biol- ogy of T. pilosa more resembles that of a terrestrial insect than an aquatic one. ACKNOWLEDGMENTS We thank: Stuart Neff, Temple University, and Brett Merritt, Michigan State University, for assistance in collecting specimens; Jack Stanford and the staff of the Flathead Lake Bio- logical Station of The University of Montana for their assistance and use of facilities; Powder River High School (Broadus, Montana) for use of facilities; the students at Powder River High School for their encouragement to continue this project; Kenneth Hagen and Leo Caltagirone, University of California, Berkeley, for their comments on the manuscript; and Jim Gustin for permission to use his observations. We dedicate our efforts on this study to the late Professor Kenneth Hagen. LITERATURE CITED Bergey, E. A. 1995. Local effects of a sedentary grazer on stream algae. Freshwat. Biol. 33:401- 409. Chapman, R. F. 1982. The insects - structure and function. 3rd ed. Harvard University Press, Cambridge. Dasch, C. E. 1979. Ichneumon-flies of America North of Mexico: 8. Subfamily Cremastinae. Mem. Amer. Entomol. Inst. 29: 1 -702. Elliott, J. M. 1982. The life cycle and spatial distribution of the aquatic parasitoid Agriotypus armatus (Hymenoptera: Agriotypidae) and its caddis host Silopallipes (Trichoptera: Goeridae). J.Anim.Ecol. 51:923-941. Hagen, K. S. 1964. Developmental stages of parasites p. 168-246. In: DeBach, P. (ed.). Biologi- cal control of insect pests and weeds. Reinhold Publ. Corp., N.Y. Hagen, K. S. 1996. Aquatic Hymenoptera. p. 474-483. In: R. W. Merritt and K. W. Cummins (eds.). An introduction to the aquatic insects of North America. 3rd ed. Kendall/Hunt Publ. Co., Dubuque, IA. Lange, W. H. Aquatic and semiaquatic Lepidoptera. p. 387-398. In: R. W. Merritt and K. W. Cummins (eds.). An introduction to the aquatic insects of North America. 3rd ed. Kendall/ Hunt Publ. Co., Dubuque, IA. Lloyd, J. T. 1914. Lepidopterous larvae from rapid streams. J. N. Y. Entomol. Soc. 2: 145-152. Lloyd, J. T. 1919. An aquatic dipterous parasite, Ginglymyia acrirostris Towns, and additional notes on its lepidopterous host, Elophilafulicalis. J. N. Y. Entomol. Soc. 27:263-265. McAuliffe, J. R. and N. E. Williams. 1983. Univoltine life cycle of Paragyractis confusalis Walker (Lepidoptera: Pyralidae) in the northern part of its range. Am. Midi. Nat. 110:440- 443. Morris, K. R. S. 1937. The prepupal stage in Ichneumonidae, illustrated by the life history of Exenterus abruptoriusThb. Bull. Entomol. Res. 28:525-534. Munroe, E. G. 1972-1973. Pyraloidea. Pyralidae (in part), p. 1-134, Fasc. 13.1, A-C. In: R. B. Dominick (ed.). The moths of America north of Mexico. E. W. Classey Ltd., London. Resh, V. H. and W. Jamie-son 1 988. Parasitism of the aquatic moth Petrophila confusalis (Lepi- doptera: Pyralidae) by the aquatic wasp Tanychela pilosa (Hymenoptera: Ichneumonidae). Entomol. News 99: 185- 188. Tuskes, P. M. 1977. Observations on the biology of Paragyractis confusalis, an aquatic pyralid. Can. Entomol. 109:695-699. Tuskes, P. M. 1981. Factors influencing the abundance and distribution of the aquatic moths of the genus Paragyractis (Pyralidae). J. Lepid. Soc. 35: 161-168. Ullyett, G. C. 1944. On the function of the caudal appendage in primary larvae of parasitic Hymenoptera. J. Entomol. Soc. S. Afr. 7:30-37. Vol. 109, No. 5, November & December, 1998 339 NEW OHIO RECORDS OF CORIXIDAE (HEMIPTERA)1 Stephen W. Chordas, III2, Brian J. Armitage2 ABSTRACT: One new genus and five new species of corixids have recently been recorded from Ohio as part of a comprehensive survey of adult aquatic insects. The total number of corixid taxa now known from Ohio is 25. All five taxa were collected using black light traps. The newly reported genus, Corisella, is known mainly from western North America. Corisella inscripta has heretofore only been found west of the Mississippi River. The other four new species records for Ohio represent three genera and include Palmacorixa buenoi, Sigara dejecta, S. hubbelli, and Trichocorixa kanza. All five new taxa were collected on one or more of the following State Wildlife Areas: Big Island, Killdeer Plains, and Resthaven. There are almost 80 State Wildlife Areas in Ohio. These are managed by the Ohio Division of Wildlife (the Division) for a variety of hunting, fishing, recreational, and watchable wildlife functions. However, few of these wildlife areas have been surveyed for their aquatic insect fauna. Recently the Division funded the Ohio Biological Survey (the Survey) to conduct a three year study of the state-listed, special category species for Killdeer Plains Wildlife Area. The Survey is also conducting a multi-year comprehensive survey of the adult aquatic insects in Ohio co-funded by the Division, the Partnerships for Wild- life Foundation of the U.S. Fish and Wildlife Service, and the Procter and Gamble Co., Inc. Finally, the Wisconsin Department of Natural Resources, with funding from Partnerships for Wildlife, has contracted with the Survey to conduct a six-state prairie Lepidoptera study. All of these projects generated aquatic insect collections from state wildlife areas, especially Big Island (Marion County), Killdeer Plains (Wyandot County), and Resthaven (Erie County). The aquatic hemipteran fauna within Ohio, including the family Corixidae, is poorly known. There have been no previous studies pertaining specifically to the aquatic members of this order in Ohio. This void is evident when com- prehensive aquatic Hemiptera works, such as Truxal's (1953) revision of the genus Buenoa (Hemiptera; Notonectidae), contained no Ohio material. How- ever, efforts such as that of Williams et al. (1996), Chordas (in review), this paper, and the current survey of adult aquatic insects in Ohio, are addressing this deficiency. Until recently, only 20 species of the family Corixidae (In- secta: Hemiptera) were known from Ohio. These included the genera (# spe- cies): Hesperocorixa (9); Palmacorixa (3); Rhamphocorixa (1); Sigara (5); and, Trichocorixa (2). In contrast, there have been 49 species, representing 9 genera, of water boatmen reported from Wisconsin (Hilsenhoff, 1984). 1 Received February 6, 1998. Accepted May 10, 1998. 2 Ohio Biological Survey and Environmental Science Program, 1735 Neil Avenue Room 103, Columbus, OH 432 10. ENT. NEWS 109(5): 339-342, November & December, 1998 340 ENTOMOLOGICAL NEWS In this paper we report five new Ohio records of water boatmen, represent- ing one new genus and five new species. This is one of a series of papers about this family and order which will culminate in a comprehensive diagnostic at- las for aquatic Hemiptera in Ohio. METHODS Adult corixids were collected from May through October using black light traps. Specimens from Killdeer Plains and Resthaven Wildlife Areas were col- lected during a survey of prairie moths. Specimens from Big Island Wildlife Area were collected during a study of aquatic insect dispersal to proposed reconstructed wetland areas. Identifications were made using keys and descrip- tions in Hungerford ( 1 948) and Sailer ( 1 948). Polhemus et al. ( 1 988) was used as the reference for known distributional records. Specimens are preserved in 75% ethanol and deposited in the Ohio Biological Survey's Aquatic Insect Collection. RESULTS All species collected on Big Island, Killdeer Plains, and Resthaven Wild- life Areas are presented in Table 1 . The new genus and species records for Ohio are indicated by symbols. Additional county records from sites outside of the three wildlife areas are provided in table footnotes. Table 1. Corixids found on three state wildlife areas. Wildlife Areas Species Big Island Killdeer Plains Resthaven T* Corisella inscripta (\Jh\er), 1894a XXX Hesperocorixa atopodonta (Hungerford), 1927 X H. obliqua (Hungerford), 1925 XXX * Palmacorixa buenoi Abbott, 191 3b X Ramphocorixa acuminata (Uhler), 1897 X X Sigara alternate (Say), 1825 XXX * S. dejecta Hungerford, 1948C X X 5. grossolineata Hungerford, 1 948 X * 5. hubbelli (Hungerford), 1928d X 5. modesta (Abbott), 1916 X Trichocorixa calva (Say), 1832 XXX * T.kanza Sailer, 1948e XXX T. sexcincta (Champion), 1901 X X t = Denotes a previously unreported genus for Ohio * = Denotes a new state record for Ohio a Also identified from Madison County " Also identified from Clermont County c Also identified from Athens, Clermont, and Greene counties d Also identifed from Franklin and Jackson counties (kicknet samples) e Also identified from Ashtabula, Clermont, Morgan, Pickaway, Richland, Tuscarawas, Washington, and Williams counties. Vol. 109, No. 5, November & December, 1998 341 DISCUSSION The five new state records increase the total state list of corixids by 25% to 25 species. This relatively large increase in the faunal list, from a small num- ber of sites, supports the notion that Ohio is undercollected. Given the distri- butions published by Polhemus et al. ( 1 988), approximately 1 0 additional taxa, primarily in the genus Sigara, could possibly be found in Ohio. Furthermore, Chordas (in review) specifically discusses potentially resident species belong- ing to the genus Hesperocorixa that may ultimately be found in Ohio. The Till Plains physiographic province in western Ohio contains remnants of midwestem prairies which once were common. Killdeer Plains and Big Island Wildlife Areas contain some of these relict areas. Additional collecting in these and other similar areas could reveal other taxa whose distribution is normally con- sidered more typical of the prairie states west of the Mississippi River. Ohio has portions of four other physiographic provinces (Lake Plains, Bluegrass, Glaciated Allegheny Plateau, and Unglaciated Allegheny Plateau) which also could contain taxa typical to these regions in other states but heretofore not collected or identified from Ohio. Sigara defecta: Ohio lies along the very southern edge of this species' midwestern range (Hungerford, 1 948; Polhemus et al. , 1 988). This species has been previously reported only from two states bordering Ohio (Michigan and Pennsylvania). However, its occurrence in two widely spaced wildlife areas suggests that it is likely to be found in additional Ohio locations. Sigara hubbelli: This species has previously been reported for all of the states bordering Ohio (Indiana, Kentucky, Pennsylvania, and West Virginia) except Michigan to the north. Based on the distributional data presented by both Hungerford (1948) and Polhemus et al. (1988), Ohio lies well within its known range and it is no surprise that it has been found within the State. A single male specimen was taken from Killdeer Plains Wildlife Area in Wyandot County. Although the single male specimen was taken by black light sam- pling, several specimens taken by dipnet sampling, from two additional locali- ties within Ohio (Table 1 ), are in the first author's private collection. This fur- ther serves to establish the presence of this species within Ohio. Trichocorixa kanza: The report of this species in Ohio extends the north- em distribution of this primarily southern species. Of the five new species reported from Ohio, this species was taken in the largest numbers and was one of the least anticipated. It has been taken from only one state, Pennsylvania, bordering Ohio. In addition to its historical distribution in the southeastern United States (Polhemus et al., 1988), the northern records of this species in- clude the District of Columbia, Delaware, Maryland, Pennsylvania, and Wis- consin (Hilsenhoff, 1984; Polhemus et al, 1988). Palmacorixa buenoi: This species has previously been reported for all states bordering Ohio (Indiana, Michigan, Pennsylvania, and West Virginia), except 342 ENTOMOLOGICAL NEWS Kentucky to the south. Its reported distribution extends from Ontario south to Florida and west to Iowa (Polhemus et al., 1988). This vast distribution, and records for bordering states, clearly place Ohio within its range. Corisella inscripta: Historical records for the genus Corisella are prima- rily from western North America (Hungerford, 1948). Two species of this ge- nus have been recorded from states east of the Mississippi River: Corisella edulis (Champion), 1901 and Corisella tarsalis (Fieber), 1851 (Hilsenhoff, 1984; Hungerford, 1948; Polhemus et al., 1988). Prior to this paper, the east- ernmost records of Corisella inscripta were from Missouri (Polhemus et al., 1988) and a single male specimen from Arkansas (Cochran and Harp, 1990). Corisella inscripta represents not only a new genus for Ohio, but also a con- siderable eastern geographical range extension. ACKNOWLEDGMENTS Funding for projects from which this work is derived came from the Ohio Division of Wildife's "Do Something Wild!" Income Tax Check-off Program, U.S. Fish & Wildlife Service's Partner- ships for Wildlife Program, and the Procter & Gamble Co., Inc. Special thanks to George L. Harp, Arkansas State University and Benjamin A. Foote, Kent State University for preliminary review of this manuscript. Suggestions by two anonymous reviewers further improved the paper. LITERATURE CITED Chordas, S.W. III. 1998. First report ofHesperocorixa semilucida (Hemiptera: Corixidae) from Ohio withnotes on distribution, habitat, and morphology. Ohio Biol. Survey Notes Series (in review). Cochran, B.C. and G.L. Harp. 1990. The aquatic macroinvertebrates of the St. Francis Sunken Lands in northeast Arkansas. Proc. Ark. Acad. Sci. 44:23-27. Hilsenhoff, W. L. 1984. Aquatic Hemiptera of Wisconsin. Great Lakes Entomol. 17( 1 ):29-50. Hungerford, H.B. 1948. The Corixidae of the Western Hemisphere (Hemiptera). Univ. Kans.Sci. Bull. 32:1-827. Polhemus, J.T., R.C. Froeschner, and D.A. Polhemus. 1988. Family Corixidae Leach, 1815 the water boatman. Pages 93-120. In: T.J. Henry and R.C. Froeschner (eds.), Catalog of the Heteroptera, or true bugs, of Canada and the continental United States. E.J. Brill. New York. 958 pp. Sailer, R.I. 1948. The genus Trichocorixa (Corixidae, Hemiptera). Pages 289-407. In: H.B. Hungerford (ed.), The Corixidae of the Western Hemisphere (Hemiptera). Univ. Kans. Sci. Bull. 32:1-827. Truxal, F.S. 1953. A revision of the genus Buenoa. Univ. Kans. Sci. Bull. 35:1351-1523. Williams, R.N., M.S. Ellis, and D.S. Fickle. 1996. Insects in the Killbuck Marsh Wildlife Area, Ohio: 1994 survey. Ohio J. Sci. 96(3):34-40. Vol. 109, No. 5, November & December, 1998 343 TWO CORRECTIONS: ARADIDAE, TINGIDAE (HETEROPTERA)1 Richard C. Froeschner^ ABSTRACT: (1) Makes modification of captions and text to correct transposed captions for Chelonoderus stylatus and Isodermus gayi in world list of flat bugs. (2) Rewrites couplets 1 9-22 of key to world genera of lace bug tribe Phatnomatini to properly place genus Minitingis. ( \ ) Kormilev and Froeschner 's ( 1 987) "Flat Bugs of the World" [Entomogr., 5:1-246] contains a transposition of names of the figures on pages 220 and 221. The illustration on page 220 should be labeled "Fig. 2. Chelonoderus stylatus Usinger. SEE p. 122."; and the illustration on page 221 should be labeled "Fig. 3. Isodermus gayi (Spinola). SEE p. 94." This necessitates the following changes in the text: on p. 94 the reference to the figure for Isodermus gayi should read "Fig. 3 (p. 221 )"; and on p. 1 22 the reference for the figure of Chelonoderus stylatus should read "Fig. 2 (p. 220)." (2) In Froeschner,s (1996) "Lace Bug Genera of the World" [Smithson. Contr. Zool, 574:i-iv, 1 -44] the genus Minitingis is misplaced within the key to the world genera of the tribe Phatnomatini (pages 17-19). Couplets 1 9-22 must be modified to read as follows: 19. Abdomen ventrally on basal half or more with a distinctly impressed groove along midline 20 Abdomen ventrally not distinctly impressed along midline 21 20. Paranotum narrowest opposite humerus, thence widened cephalad to 4 or more row of cells Phatnoma Fieber Paranotum anteriorly not or only slightly (1-2 cells) widened Minitingis Barber 2 1 . Head with a clypeal spine 22 Head without a clypeal spine 25 22. Occipital spines nearly or quite as long as horizontal diameter of an eye Gonycentrum Bergroth Occipital spines absent or much shorter than horizontal diameter of an eye 23 Couplets 23-25 as published. ACKNOWLEDGMENTS Thanks are extended to T. J. Henry (U.S.D.A. -Systematic Entomology Laboratory) and P. J. Spangler, Smithsonian Institution for reviews of this paper. 1 Received May 29, 1998. Accepted June 13, 1998. 2 Department of Entomology, MRC- 105, National Museum of Natural History, Washington, D.C., 20560. ENT. NEWS 109(5): 343, November & December, 1998 344 ENTOMOLOGICAL NEWS NEW NAME FOR A GENERIC HOMONYM IN TELOGANODIDAE (EPHEMEROPTERA)1*2 W. P. McCafferty3, T.-Q. Wang4 McCafferty and Wang ( 1 997) proposed the name Nadinella McCafferty and Wang for two species of South African mayflies (family Teloganodidae) that represented a new distinctive genus with numerous diagnostic features, including, in the larvae, possession of two rows of denticles on the claws and lamellate gills on abdominal segments 2-5. These mayflies had previously been considered in the genus Ephemerellina Lestage. John Page of Great Britain has kindly informed us by letter, however, that the name Nadinella is preoccu- pied by a fossil protozoan described in 1 899 by Eugene Penard (Penard 1 899). That name was more recently listed in Moore (1964), but we had missed the citation in the Zoological Record and are not schooled in general invertebrate paleontology. We therefore propose Nadinetella McCafferty and Wang, nomen novum for Nadinella McCafferty and Wang, 1 997: 399, nee Nadinella Penard, 1 899: 82. The name Nadinetella incorporates the given name of Nadine McCafferty, for whom it is named and who was of considerable aid in collecting specimens of the new genus in South Africa. LITERATURE CITED McCafferty, W. P. and T.-Q. Wang. 1 997. Phylogenetic systematics of the family Teloganodidae (Ephemeroptera: Pannota). Ann. Cape Provincial. Mus., Natural Hist. 19: 387-437. Moore, R. C. (ed.). 1964. Treatise on invertebrate paleontology. Part C Protista 2. Sarcodinia, chiefly 'thecamoebians' and Foraminifera. Volumes 1 & 2. Geol. Soc. Am., Lawrence, Kan- sas. Penard, E. 1899. Les Rhizopodes de faune profonde dans le Lac Leman. Rev. Suisse Zool. 7: 1-142. ' Received and accepted August 10, 1998. 2 Purdue Agr. Res. Prog. J. No. 16784. 3 Dept. of Entomology, Purdue University, West Lafayette, IN 47907. 4 23 Marsh Creek Dr., Mauldin, SC 29662. ENT. NEWS 109(5): 344, November & December, 1998 Vol. 109, No. 5, November & December, 1998 345 A NEW NORTH AMERICAN GENUS OF BAETIDAE (EPHEMEROPTERA) AND KEY TO BAETIS COMPLEX GENERA1'2 C. R. Lugo-Ortiz, W. P. McCafferty3 ABSTRACT: Plauditus, n. gen. (Ephemeroptera: Baetidae) is established for certain North American Baetis complex species that lack hindwingpads and a developed median caudal fila- ment in the larval stage and that were recently transferred from Pseudodoeon to Baetis or Barbaetis. Thus, P. alachua, n. comb., P. armillatus, n. comb., P. bimaculatus, n. comb., P. cestus, n. comb., P. cinctutus, n. comb., P. dubius, n. comb., P. elliotti, n. comb., P.futilis, n. comb., P. punctiventris, n. comb., P. rubrolateralis, n. comb. P. veteris, n. comb., and P. virilis, n. comb, are contained in the new genus. Plauditus cestus is the type species. The larval stage of Plauditus is differentiated from other genera of the Baetis complex by numerous character- istics, and the adult stage is differentiated from other North American baetids also having double marginal intercalaries in the forewings and lacking hindwings (certain Acentrella, Apobaetis, and Paracloeodes). A new, simplified, and illustrated generic key to the North American Baetis complex larvae is provided. Small minnow mayflies (Ephemeroptera: Baetidae) are relatively well known in North America north of Mexico, with 143 species among 20 genera currently reported (McCafferty 1996, 1997a; see also McCafferty and Silldorff 1998, Wiersema 1998, Wiersema and McCafferty 1998). Larvae are distin- guished by having the initial lateral branches of the epicranial suture located anterior to (below) the lateral ocelli (Wang and McCafferty 1996: Figs. 1-6) and the femoral apices with a ventrally oriented dorsal lobe (Wang and McCafferty 1996: Figs. 13-16). Adults are distinguished by having forewing veins IMA, MA2, IMP, and MP2 basally detached, and three-segmented mid- and hindtarsi. Male adults are further distinguished by the presence of mem- branous penes and turbinate compound eyes (the South American genus Aturbina Lugo-Ortiz and McCafferty [1996] lacks turbinate compound eyes). Despite being easily recognized at the family level, several North American baetid species have been difficult to assign to genera. This situation is prima- rily due to the fact that reductive trends and relative high frequency of ho- moplasy within the family have limited the number of reliable diagnostic char- acteristics of use at the genus level. North American species previously assigned to Pseudodoeon Klapalek are examples that have required revised generic placement as our knowledge 1 Received January 22, 1998. Accepted March 13, 1998. 2 Purdue Agricultural Research Program Journal No. 15576. •* Department of Entomology, Purdue University, West Lafayette, IN 47907. ENT. NEWS 109(5): 345-353, November & December, 1998 346 ENTOMOLOGICAL NEWS of genus level systematics has improved. North American species once as- signed to Pseudocloeon have the following combination of characteristics: absence of hindwings (and hindwingpads), presence of double marginal intercalaries in the forewings, and presence of a highly reduced medial caudal filament in the larval stage. As a result of the restriction of the concept of Pseudocloeon to its Oriental type (Waltz and McCafferty 1985, 1987), all North American species once considered to belong to Pseudocloeon were transferred toApobaetis Day (Waltz and McCafferty 1986), Acentrella Bengtsson (Waltz and McCafferty 1987), Baetis Leach (McCafferty and Waltz 1990), and Barbaetis Waltz and McCafferty (McCafferty and Waltz 1990). Our research indicates that all 1 1 species of Pseudocloeon that were provi- sionally assigned to Baetis by McCafferty and Waltz (1990) and also Barbaetis cestus (Provonsha and McCafferty) (originally described in Pseudocloeon) represent a new Baetis-comp\c\ genus distinguishable by numerous charac- teristics. We herein describe the new genus and provide a revised generic key to the North American larvae of the Baetis complex. Plauditus Lugo-Ortiz and McCafferty, NEW GENUS Larva. Head: Antennae as long as head capsule or longer; scapes without distal notch, subequal in length in length to pedicels (Fig. 1 ). Labrum (Fig. 2) broadly rounded anteriorly, with anteromedial notch. Hypopharynx (Fig. 3) with lingua and superlinguae broadly rounded apically. Left mandible (Fig. 4) with incisors fused; prostheca apically broad and denticulate. Right mandible with incisors apically fused (Fig. 5) or slightly cleft; prostheca slender, apically denticulate. Maxillae (Fig. 6) with palps two segmented. Labium (Fig. 7) compact; glossae slightly shorter than paraglossae, apically narrower than base; paraglossae broad, broadly rounded apically; palps three segmented; palp segment 1 slightly longer than segments 2 and 3 com- bined; palps segment 2 as long as or slightly longer than segment 3; segment 3 relatively broad apically, approaching truncate, sometimes appearing slightly concave, medial margin straight to slightly convex distally (never convergent apically from base). Thorax: Hindwingpads ab- sent. Legs (Fig. 8) with femora with villopore present (Fig. 9) and dorsal row of long, robust setae. Tarsal claws (Fig. 10) somewhat elongate and with only weak curvature, with one row of long, sharp denticles. Abdomen: Gills on abdominal segments 1 -7, broadly rounded, untracheated or poorly tracheated, marginally smooth. Terga (Fig. 1 1 ) creased, with minute, sharp, triangular spines, or with minute, fine, simple setae scattered over surface. Cerci with abundant fine, simple setae medially. Medial caudal filament subequal in length to abdominal segment 10. Adult. Head: Male compound eyes circular, somewhat enlarged. Male foretibiae 1.3-1.5x length of femora. Mesoscutum with small, rounded anterior process in lateral view (Fig. 12). Forewings with paired marginal intercalaries. Hindwings absent. Genital forceps (Fig. 13) three segmented; segment 1 distomedially produced; segment 2 slender, strongly arched; segment 3 slender, ellipsoidal. Type species. Pseudocloeon cestum Provonsha and McCafferty. Vol. 109, No. 5, November & December, 1998 347 Figs, l-l I. Plauditus punctiventris. 1. Antennal scape and pedicel. 2. Labrum (dorsal). 3. Hy- popharynx. 4. Left mandible. 5. Right mandible. 6. Left maxilla. 7. Labium (left-ventral; right- dorsal). 8. Right foreleg (posterior face). 9. Villopore. 10. Tarsal claw. 1 1. Detail of tergum 4. 12. Adult mesoscutum (lateral). 13. Male genitalia. 348 ENTOMOLOGICAL NEWS Included species. (In addition to the recombined species listed below, a new species is presently being described by McCafferty and Waltz [1998], and one other is in manuscript [R. D. Waltz, pers. comm.]). Plauditus alachua (Berner), n. comb. Pseudocloeon alachua Berner 1940:58 (larva; male, female adults). Baetis alachua (Berner): McCafferty and Waltz 1990:775. Plauditus armillatus (McCafferty and Waltz), n. comb. Pseudocloeon parvulum McDunnough 1932:210 (larva; male, female adults), [secondary homonym]. Baetis armillatus McCafferty and Waltz 1990:775. [renamed]. Plauditus bimaculatus (Bemer), n. comb. Pseudocloeon bimaculatum Berner 1946:79 (larva; male, female adults). Baetis bimaculatus (Bemer): McCafferty and Waltz 1990:775. Plauditus cestus (Provonsha and McCafferty), n. comb. Pseudocloeon cesium Provonsha and McCafferty 1982:28 (larva; male, female adults). Barbaetis cestus (Provonsha and McCafferty): McCafferty and Waltz 1990:777. Plauditus cinctutus (McCafferty and Waltz), n. comb. Pseudocloeon cingulatum McDunnough 1931:85 (male, female adults); Ide 1937:236 (larva), [secondary homonym]. Baetis cinctutus McCafferty and Waltz 1990:776. '[renamed]. Plauditus dubius (Walsh), n. comb. Cloeon dubium Walsh 1 862:380 (male, female adults). Pseudocloeon dubium (Walsh): McDunnough 1924b: 115; Ide 1937:237 (larva). Pseudocloeon chlorops McDunnough 1923:45. Baetis dubius (Walsh): McCafferty and Waltz 1990:775. Plauditus elliotti (Daggy), n. comb. Pseudocloeon elliotti Daggy 1945:392 (male, female adults). Baetis elliotti (Daggy): McCafferty and Waltz 1990:775. Plauditus futilis (McDunnough), n. comb. Pseudocloeon futile McDunnough 1931 :86 (male, female adults). Baetis futile (McDunnough): McCafferty and Waltz 1990:775. Baetis futilis (McDunnough): McCafferty 1997b:318. Plauditus punctiventris (McDunnough), n. comb. Pseudocloeon punctiventris McDunnough 1923:45 (male, female adults); Ide 1937:237 (larva). Pseudocloeon anoka Daggy 1945:391. Pseudocloeon edmundsi Jensen 1969: 14. Pseudocloeon myrsum Burks 1953: 139. Baetis punctiventris (McDunnough): McCafferty and Walt/. 1990:776. Plauditus rubrolateralis (McDunnough), n. comb. Pseudocloeon rubrolaterale McDunnough 1931:86 (male, female adults). Baetis rubrolaterale (McDunnough): McCafferty and Waltz 1990:776. Baetis rubrolateralis (McDunnough): McCafferty 1997a:318. Plauditus veteris (McDunnough), n. comb. Pseudocloeon veteris McDunnough I924a:8 (male, female adults). Baetis veteris (McDunnough): McCafferty and Waltz 1990: 776. Plauditus virilis (McDunnough), n. comb. Cloeon virile McDunnough 1923:46 (male, female adults). Pseudocloeon virile (McDunnough): McDunnough I924b: 1 16; Ide 1937:239 (larva). Baetis virile (McDunnough): McCafferty and Waltz 1990:776. Baetis virilis (McDunnough): McCafferty 1997b:3l2. Vol. 109, No. 5, November & December, 1998 349 Distribution. Species of Plauditus are presently known from the conter- minous USA and the lower tier of provinces of Canada. There remains the possibility that the genus may eventually be found in Mexico because it is presently well represented in Texas (Lugo-Ortiz and McCafferty 1995, Wiersema and McCafferty 1998). It is also possible that Plauditus is repre- sented in the eastern Palearctic by certain species that otherwise may have been variously regarded as Acentrella, Baetis, or Pseudocloeon. We have no evidence of this at the present. Etymology. The generic name is an arbitrary combination of Latin letters having a transliteral meaning of "little applause." Diagnosis. The presence of the larval femoral villopore indicates that Plauditus is a member of the Baetis complex of genera, represented in North America north of Mexico by Acentrella, Baetis, Barbaetis, Heterocloeon McDunnough, and Labiobaetis Novikova and Kluge. Within that complex, larvae of Plauditus are distinguished by the following combination of charac- teristics: lacking a medial field of setae dorsally on the labrum (Fig. 2); having the right mandible with an apically denticulate prostheca (Fig. 5); lacking a protuberance on the apex of maxillary palp segment 3 (Fig. 6); having glossae shorter than paraglossae, relatively broad paraglossae, and a subquadrate palp segment 3 (Fig. 7); lacking dorsal setae on the apical half of the glossae (viz. well-developed medial aspect) (Fig. 7); lacking hindwingpads; lacking procoxal osmobranchia; lacking clavate setae on the dorsal margin of the tibiae and tarsi (Fig. 8); and having a highly reduced medial caudal filament (Provonsha and McCafferty 1 982: Fig. 8). A more detailed diagnosis of Plauditus larvae in relation to those of other Baetis-comp\e\ genera can be performed using the identification key provided below. Adults of Plauditus are similar to hindwingless Acentrella, Apobaetis Day, and Paracloeodes Day. Adults of Plauditus differ from those of Acentrella in having a small, rounded anterior process on the mesoscutum (Fig. 12), and from those of Apobaetis by lacking a subconical process between the male genital forceps (Fig. 1 3). Adults of Plauditus differ from those of Paracloeodes in that segment 2 of the male genital forceps is slightly produced distomedially and tends to be more slender and strongly arched (Fig. 13). 350 ENTOMOLOGICAL NEWS KEY TO LARVAE OF NORTH AMERICAN BAETIS-COMPLEX GENERA 1 . Both mandibles lacking tuft of setae between incisors and prostheca (Figs. 4, 5); villopore usually apparent on femora (Fig. 9); claws never approaching or exceeding length of tarsi (Figs. 8, 10) Baetis complex, 2 One or both mandibles with tuft of setae between incisors and prostheca (Fig. 14); villopore absent from femora; claw length variable other Baetidae 2. Antennal scapes with distal notch (Fig. 15); maxillary palp segment 2 with subapical excavation (Fig. 16); hindwingpads present; median caudal filament developed Labiobaetis Antennal scapes without distal notch (Fig. 1); maxillary palp segment 2 without subapical excavation (Fig. 6); hindwingpads present or absent; median caudal filament developed or highly reduced 3 3. Median caudal filament developed; hindwingpads present 4 Median caudal filament reduced; hindwingpads present or absent 5 4. Antennae approximately twice length of head capsule Baetis Antennae subequal in length to head capsule Barbaetis 5. Procoxae with (Fig. 19) or without gills; claws with two rows of denticles (second row minute and sometimes conspicous only under high magnification) Heterocloeon Procoxae without gills; claws with one row of denticles 6 6. Hindwingpads absent 7 Hindwingpads present, sometimes minute 8 7. Femora, tibiae, and tarsi without row of long setae (Fig. 8); labial palp segment 3 subquadrate, with medial margin almost straight (not receding from base) and sometimes appearing slightly convex apically (Fig. 7) Plauditus Femora, tibiae, and tarsi with row of long setae (Fig. 18); labial palp segment 3 apically rounded, with medial margin receding from base (Fig. 17) Acentrella 8. Labial palp segment 2 with well-developed distomedial thumb (Fig. 20); abdominal tergal scales present (Fig. 21) Baetis Labial palp without well-developed distomedial thumb (Fig. 17); abdominal tergal scales absent Acentrella Vol. 109, No. 5, November & December, 1998 351 14 15 16 17 19 21 20 Figs. 1 4-2 1 . 1 4. Fallceon quilleri, left mandible (pointer towards setal tuft) (modified from Lugo- Ortiz et al. [1994]). 15-16. Labiobaetis propinquus 15. Antennal scape and pedicel (pointer to- wards notch) (modified from McCafferty and Waltz [1995]). 16. Left maxilla (pointer towards excavation) (modified from McCafferty and Waltz [1995]). 17-18. Acentrella turbida. 17. La- bium (modified from McCafferty et al. [1994]). 18. Right foreleg (modified from McCafferty et al. [1994]). 19. Heterocloeon berneri, forecoxal gill (pointer towards gill) [modified from Miiller- Liebenau [1974]). 20-21. Baetis bicaudatus, labial palp (pointer towards thumb) (modified from Morihara and McCafferty [1979]). 21. Detail of tergum 4. 352 ENTOMOLOGICAL NEWS ACKNOWLEDGMENTS We thank R. D. Waltz (Department of Natural Resources, Indianapolis, Indiana) for discuss- ing this paper, and A. V. Provonsha and R. P. Randolph (Purdue University, West Lafayette, Indiana) for preliminary review of the manuscript. LITERATURE CITED Berner, L. 1940. Baetine mayflies from Florida (Ephemeroptera). Fla. Entomol. 23: 33-45, 49- 62. Berner, L. 1946. New species of Florida mayflies (Ephemeroptera). Fla. Entomol. 28: 60-82. Burks, B. D. 1953. The mayflies, or Ephemeroptera, of Illinois. Bull. 111. Nat. Hist. Surv. 26: 1 - 216. Daggy, R. H. 1945. New species and previously undescribed naiads of some Minnesota may- flies (Ephemeroptera). Ann. Entomol. Soc. Am. 38: 373-396. Ide, F. P. 1937. Descriptions of eastern North American species of baetine mayflies with particu- lar reference to the nymphal stages. Can. Entomol. 69: 219-231, 235-243. Jensen, S. L. 1969. A new species of Pseudocloe on from Idaho. Pan-Pac. Entomol. 45: 14-15. Lugo-Ortiz, C. R. and W. P. McCafferty. 1995. The mayflies (Ephemeroptera) of Texas and their biogeographic affinities. Pp. 151-169. In: L. D. Corkum and J. J. H. Ciborowski (eds.), Current directions in research on Ephemeroptera. Canadian Scholars' Press, Toronto. Lugo-Ortiz, C. R. and W. P. McCafferty. 1996. Aturbina georgei gen. et sp. n.: a small minnow mayfly (Ephemeroptera: Baetidae) without turbinate eyes. Aq. Insects 18: 175-183. Lugo-Ortiz, C. R., W. P. McCafferty, and R. D. Waltz. 1994. Contribution to the taxonomy of the Panamerican genus Fallceon (Ephemeroptera: Baetidae). J. N. Y. Entomol. Soc. 102: 460-475. McCafferty, W. P. 1996. The Ephemeroptera species of North America and index to their com- plete nomenclature. Trans. Entomol. Soc. Am. 122: 1-54. McCafferty, W. P. 1997a. Ephemeroptera. Pp. 89-117. In: R. W. Poole and P. Gentili (eds.), Nomina Insecta Nearctica: a checklist of the insects of North America, Vol. 4: Non-holom- etabolous orders. Entomol. Info. Services, Rockville, Maryland. McCafferty, W. P. 1997b. Name adjustments and a new synonym for North American Ephemeroptera species. Entomol. News 108: 318-319. McCafferty, W. P. and E. L. Silldorff. 1998. Reared association and equivalency of Baetis adonis and caelestis (Ephemeroptera: Baetidae). Entomol. News 109: 261-265. McCafferty, W. P. and R. D. Waltz. 1990. Revisionary synopsis of the Baetidae (Ephemeroptera) of North and Middle America. Trans. Am. Entomol. Soc. 1 16: 769-799. McCafferty, W. P. and R. D. Waltz. 1995. Labiobaetis (Ephemeroptera: Baetidae): new status, new North American species, and related new genus. Entomol. News 106: 19-28. McCafferty, W. P. and R. D. Waltz. 1998. New species of the small minnow mayfly genus Plauditus (Ephemeroptera: Baetidae) from South Carolina. Entomol. News 109: 354-356. McCafferty, W. P., M. J. Wiggle and R. D. Waltz. 1994. Systematics and biology ofAcentrelta turbida (McDunnough) (Ephemeroptera: Baetidae). Pan-Pac. Entomol. 70: 301-308. McDunnough, J. 1923. New Canadian Ephemeridae with notes. Can. Entomol. 55: 39-50. McDunnough, J. 1924a. New Ephemerida from Illinois. Can. Entomol. 56: 7-9. McDunnough, J. 19245. New Canadian Ephemeridae with notes II. Can. Entomol. 56: 90-98, 113-122, 128, 133. McDunnough, J. 1931. New species of Canadian Ephemeroptera. Can. Entomol. 63: 82-93. McDunnough, J. 1932. New species of North American Ephemeroptera, II. Can. Entomol. 64: 209-215. Morihara, D. K. and W. P. McCafferty. 1979. The Baetis larvae of North America (Ephemeroptera: Baetidae). Trans. Am. Entomol. Soc. 105: 139-221. Vol. 109, No. 5, November & December, 1998 353 Miiller-Liebenau, I. 1974. Rheobaetis: a new genus from Georgia (Ephemeroptera: Baetidae). Ann. Entomol. Soc. Am. 67: 555-567. Provonsha, A. V. and W. P. McCafferty. 1982. New species and previously undescribed larvae of North American Ephemeroptera. J. Kansas Entomol. Soc. 55: 23-33. Walsh, B. D. 1862. List of the Pseudoneuroptera of Illinois contained in the cabinet of the writer, with descriptions of over 40 new species, and notes on their structural affinities. Proc. Acad. Nat. Sci. Phila. 2nd ser., pp. 361-402. Waltz, R. D. and W. P. McCafferty. 1985. Redescription and new lectotype designation for the type species of Pseudocloeon, P. kraepelini Klapalek (Ephemeroptera: Baetidae). Proc. Entomol. Soc. Wash. 87: 800-804. Waltz, R. D. and W. P. McCafferty. 1986. Apobaetis etowah (Traver), a new combination in Nearctic Baetidae (Ephemeroptera). Proc. Entomol. Soc. Wash. 88: 191. Waltz, R. D. and W. P. McCafferty. 1987. Systematics of Pseudocloeon, Acentrella, Baetiella, and Liebebiella, new genus (Ephemeroptera: Baetidae). J. N. Y. Entomol. Soc. 95: 553-568. Wang, T.-Q. and W. P. McCafferty. 1996. New diagnostic characters for the mayfly family Baetidae (Ephemeroptera). Entomol. News 107: 207-212. Wiersema, N. A. 1998. Camelobaetidius variabilis (Ephemeroptera: Baetidae), a new species from Texas, Oklahoma and Mexico. Entomol. News 109: 21-26. Wiersema, N. A. and W. P. McCafferty. 1998. A new species of Pseudocentroptiloides (Ephemeroptera: Baetidae), with revisions of other previously unnamed baetid species from Texas. Entomol. News 109: 110-116. 354 ENTOMOLOGICAL NEWS A NEW SPECIES OF THE SMALL MINNOW MAYFLY GENUS PLAUDITUS (EPHEMEROPTERA: BAETIDAE) FROM SOUTH CAROLINA1 W. P. McCafferty2, R. D. Waltz3 ABSTRACT: A newly discovered species of Baetidae (Ephemeroptera), Plauditus gloveri, n. sp., is described from larvae taken in South Carolina. Characterization or combinations thereof involving color pattern, antennal length, labral setation, maxillary palp shape and size, distal shape of the labial palps, claw curvature, and tergal spines distinguish the new species. Antennal and claw characterization suggests a possible close relationship with P. cestus. Our examination of larval material of small minnow mayflies recently taken from streams in South Carolina revealed a distinctive new species. Generic characterization of the new species place it within the recently erected genus Plauditus Lugo-Ortiz and McCafferty (1998). We are honored to name the new species after James B. Glover (Columbia, South Carolina), who collected the original material. Plauditus gloveri NEW SPECIES (Figs. 1-10) Larva. Body length, 4.5-5.0 mm; antennae length, ca. 1.0 mm; cerci length 1.8-2.0 mm. Base color pale yellow; markings light to medium brown. Head: Head capsule with submedian rows of short, irregular, transverse dashes on either side of medial trunk of epicranial suture, with pair of short transverse dashes below each compound eye, and with oblique pair of spots above each antennal base. Antennae not marked. Dorsal chetotaxy of labrum as in Fig. 1 , with pair of long, simple, hairlike, submarginal setae located approximately half way between lat- eral margin and median line of labrum, and extending beyond distal labral margin for about half length of seta (in some, extending setae slightly more proximate to each other than shown in Fig. 1). Maxillae as in Fig. 2, with maxillary palp narrow and extending beyond apex of galealacinia. Mandibles as in Figs. 3 and 4. Terminal segment of labial palp (Fig. 5) slightly broadening apically, with very slight distolateral point; apical margin slightly concave in lat- eral half, and rounded and somewhat bulbous in medial half. Thorax: Pronotum with three prominent spots (one medial, two submedial) near anterior margin. Mesonotum with mostly scattered, lateral light brown spots. Metanotum with pair of prominent submedial spots. Hindwingpads absent. Legs not generally marked; anterior face of femora with pair of dorsal and ventral dashes as in Fig. 6, dorsal dash sometimes appearing as two partially connected elongate spots. Claws (Fig. 7) relatively straight. Abdomen: Abdominal segment 7 slightly darker than other segments (possibly indicating darker segment band in other individuals), more noticeable ventrally, and perceptible on specimens only when using black background and low magnification; abdominal terga 1 -9 (Fig. 8) each with pair of dark submedian spots (lateral spots also usually present); tergum 2 with conspicuous medial V-shaped mark at ante- rior margin; tergal surfaces with weak creases and minute, simple setae; posterior margin of 1 Received January 22, 1998. Accepted March 13, 1998. 2 Department of Entomology, Purdue University, West Lafayette, IN 47907, USA. 3 IDNR, Division of Entomology and Plant Pathology, 402 West Washington, Rm. W-290, Indianapolis, IN 46204, USA. ENT. NEWS 109(5): 354-356, November & December, 1998 Vol. 109, No. 5, November & December, 1998 355 terga with slightly separated, triangular shaped spines slightly longer that basal width. Ab- dominal sterna (Fig. 9) with submedian pair of diffuse brown spots; darker medial maculation present on sterna 2-8 (prominent and larger on stema 3-5 and overlapping sterna somewhat at anterior and posterior sternal margins); lateral subdermal striations evident on sterna 1-6. Gills (Fig. 10) with margins rounded apically, smooth, and with only very few scattered, short, simple setae; gill tracheal trunk not extending much beyond midlength of gill and with lateral branches only weakly developed (details of tracheation evident only under high magnification). Cerci with three light brown bands (not always perceptible). Median caudal filament highly reduced, subequal in length to mid-dorsal length of tergum 10. Adult. Unknown. Material examined. Holotype: Larva, SOUTH CAROLINA, Cherokee County, Kings Creek @ S-l 1-209, 3 miles west of Smyrna, VI-25-1995, J. Glover (deposited in the Purdue Entomologi- cal Research Collection, West Lafayette, Indiana). Paratypes: 1 larva (broken, some pans miss- "^ 10 Figs. I -10. Plauditus gloveri. 1. Labrum (dorsal). 2. Maxilla. 3. Right mandible. 4. Left man- dible. 5. Labial palp. 6. Midfemora (anterior face). 7. Claw. 8. Abdomen (dorsal). 9. Abdomen (ventral). 10. Gill 5. 356 ENTOMOLOGICAL NEWS ing), same data and deposition as holotype; 2 larvae (mounted on slides, medium Euparol), SOUTH CAROLINA, Fairfield County, Little River® S-20-60, 3.1 miles southwest of Jenkinsville, VI- 28-1995, J. Glover (same deposition as holotype). Remarks. Larvae of Plauditus gloveri are easily distinguished from all other known larvae of Plauditus on the basis of morphological and color pat- tern characterization given above. Seven other species of Plauditus have been reported from the southeast: P. alachua (Berner), P. armillatus (McCafferty and Waltz), P. bimaculatus (Berner), P. cinctutus (McCafferty and Waltz), P. dubius (Walsh), P. punctiventris ((McDunnough), and P. rubrolateralis (McDunnough). On the basis of its relatively straight tarsal claw, P. gloveri may be closely related to P. cestus (Provonsha and McCafferty). Plauditus gloveri and P. cestus have antennae that are clearly shorter than other known species of Plauditus, although those of P. gloveri are not nearly as short as those off. cestus (Provonsha and McCafferty 1982). Plauditus gloveri, how- ever, differs from P. cestus in numerous other characteristics, including the position of the pair of extending dorsal labral setae, the relatively much longer maxillary palps, femoral markings, the longer and somewhat more pointed tergal spines, and patterning of the abdomen and cerci. Abdominal segment banding on segment 7 is evident but not well devel- oped on the specimens of P. gloveri that we have examined. It is probable that segment 7 banding will be more pronounced in larger series. All of our speci- mens were female larvae, and it is also possible that the banding as well as other patterning will be more developed in males. This possibility is based on the fact that such sexual dimorphism has been documented for certain other species in the genus Plauditus (e.g., P. dubius and P. virilis [Ide 1937]). Ab- dominal segment banding occurs on segment 5 in most specimens of P. cestus, but has never been seen on segment 7. Although all material of P. gloveri has been taken from the Broad River Basin in South Carolina, no specific ecological data are yet associated with this new species. ACKNOWLEDGMENTS We thank Jim Glover (Columbia, South Carolina) for collecting the new species and ini- tially recognizing that it did not match other known species. We especially thank Arwin Provonsha (West Lafayette, Indiana) for the illustrations used herein. This paper has been as- signed Purdue Agricultural Research Program Journal No. 15602. LITERATURE CITED Ide, F. P. 1937. Descriptions of eastern North American species of baetine mayflies with particu- lar reference to the nymphal stages. Can. Entomol. 69: 219-231, 235-243. Lugo-Ortiz, C. R. and W. P. McCafferty. 1998. A new North American genus of Baetidae (Ephemeroptera) and key to Baetis complex genera. Entomol. News 109: 345-353. Provonsha, A. V. and W. P. McCafferty. 1982. New species and previously undescribed larvae of North American Ephemeroptera. J. Kans. Entomol. Soc. 55: 23-33. Vol. 109, No. 5, November & December, 1998 357 NEW SPECIES OF CLOEON AND DEMOULINIA (EPHEMEROPTERA: BAETIDAE) FROM MADAGASCAR1'2 C. R. Lugo-Ortiz, W. P. McCafferty3 ABSTRACT: Cloeon emmanueli, new species, and Demoulinia insularis, new species, are de- scribed from larvae from Madagascar. Cloeon emmanueli represents the first bonafide report of Cloeon from the island. The species is distinguished by the setation of the labrum, abdomi- nal color pattern, tergal armature, and irregular paraproctal spines. Cloeon cambouei, C. durani, and C. irretitum are considered nomina dubia because they were described from subimagos only. Demoulinia insularis is the first species of Demoulinia to be reported from the island. The species is distinguished by the relatively wide anteromedial emargination of the labrum, eden- tate tarsal claws, and numerous paraproctal spines. The faunal composition of the small minnow mayflies (Ephemeroptera: Baetidae) of Madagascar has recently received considerable attention (Lugo- Ortiz and McCafferty 1997abef, 1998a), but requires additional study. Baetid genera that were shown in those works to occur in Madagascar include Afroptilum Gillies, Cheleocloeon Wuillot and Gillies, Dabulaman-ia Lugo- Ortiz and McCafferty, Dicentroptilum Wuillot and Gillies, Edmulmeatus Lugo- Ortiz and McCafferty, Herbrossus McCafferty and Lugo-Ortiz, Labiobaetis Novikova and Kluge, Mulelocloeon Gillies and Elouard, and Xyrodromeus Lugo-Ortiz and McCafferty. Lugo-Ortiz and McCafferty ( 1 998a) confirmed that Nesoptiloides, first described from Madagascar by Demoulin (1973), was a valid genus. Reports of adults of Centroptilum Eaton from Madagascar are, however, highly tenuous because species previously assigned to that genus in Africa have been shown to represent diverse evolutionary lineages not includ- ing Centroptilum (Gillies 1990, Wuillot and Gillies 1994, Lugo-Ortiz and McCafferty 1996abc, 1997cd, 1998ab, McCafferty et al. 1997). We expect such adults in Madagascar to be members of the Centroptiloides complex (see Lugo-Ortiz and McCafferty 1998a). Madagascar species described as Cloeon Leach are based on subimagos (Navas 1 926, 1 930, 1 936) that cannot be placed to genus with any reliability. In this paper, we describe one species of Cloeon and one species of Demoulinia Gillies based on larvae collected from Madagascar. The new spe- cies of Cloeon represents the first substantiated report of the genus from Mada- gascar, and the new species of Demoulinia is the first of that genus to be de- scribed from the island. Examined specimens are housed in the Purdue Ento- mological Research Collection, West Lafayette, Indiana. 1 Received March 11, 1998. Accepted April 4, 1998. 2 Purdue Agricultural Research Program Journal No. 15659. - Department of Entomology, Purdue University, West Lafayette, IN 47907. ENT. NEWS 109(5): 357-362, November & December, 1998 358 ENTOMOLOGICAL NEWS Cloeon emmanueli Lugo-Ortiz and McCafferty, NEW SPECIES Larva. Body length: 4.3-5.2 mm. Caudal filaments length: 3.0-4.7 mm. Head: Coloration medium brown to medium yellow-brown, with no distinct markings. Antennae approximately 3.0x length of head capsule. Labrum (Fig. 1) with numerous long, fine, simple setae scattered over surface. Hypopharynx as in Figure 2. Left mandible (Fig. 3) with seven denticles; prostheca apically denticulate; tuft of long, fine, simple setae between prostheca and mola. Right man- dible (Fig. 4) with outer set of incisors with four denticles and inner set with two denticles; tuft of long, fine, simple setae between prostheca and mola. Maxillae (Fig. 5) with irregular row of long, fine, simple setae submedially in midregion; palps three segmented; palp segment 1 ap- proximately 0.63x length of segment 2 and 3 combined; segment 2 approximately 1.45x length of segment 3; segment 3 poorly defined. Labium (Fig. 6) with glossae slightly longer than paraglossae; glossae basally broad, apically narrow, with minute, fine, simple setae ventrally near base; paraglossae broadly rounded apically, with three rows of long, fine, simple setae ventrally and few minute, fine, simple setae scattered dorsally near base; palp segment 1 ap- proximately 0.82x length of segments 2 and 3 combined; segment 2 subequal in length to segment 3, with row of five to six minute, fine, simple setae dorsally; segment 3 with numerous long, somewhat robust, simple setae scattered over surface. Thorax: Coloration medium yel- low-brown, with no distinct markings. Hindwingpads absent. Legs (Fig. 7) pale yellow-brown; femora with two rows of 10-12 robust, apically pointed setae dorsally and numerous short, stout, simple seta ventrally; tibiae with few long, fine, simple setae and one long, robust, simple setae dorsally near apex, and two rows of 15-17 robust, apically pointed, simple setae ventrally; tarsi with few, long, fine, simple setae dorsally and two rows of robust, apically pointed, simple and pectinate setae ventrally; tarsal claws (Fig. 8) with two rows of 15-20 minute to small denticles each. Abdomen: Coloration medium yellow-brown and medium brown; segments 1 and 10 medium yellow-brown; segments 2 and 3 medium yellow-brown, medium brown sublaterally; segments 4-9 medium yellow-brown, with small medium brown markings anterolaterally. Sterna medium yellow-brown; sterna 4-9 with faint medium brown broad band medially. Terga (Fig. 9) with numerous scale bases and few minute, fine, simple setae scattered over surface; posterior triangular spination irregular. Gills 1-6 with two lamel- lae, gill 7 single. Paraproct (Fig. 10) with 10-12 marginal spines and numerous minute, fine, simple setae scattered over surface. Caudal filaments pale yellow-brown, with medium brown annulations every three to four segments; terminal filament approximately 0.60x length of cerci. Adult. Unknown. Material examined. Holotype: Larva, MADAGASCAR, Antsiranana Prov., Djabala R., 1 1 km N W of Hell- Ville, Nosy Be, 25-X- 1 97 1 , G. F., C. H. Edmunds, and F. Emmanuel. Paratype: Larva, same data as holotype [mouthparts, left foreleg, tergum 4, gills 4, and paraproct of one larva mounted on slide (medium: Euparal)]. Additional material: Four larvae, same data as holotype. Etymology. We name this species after F. Emmanuel (Madagascar), who assisted in its collection. Discussion. Although no other species of Cloeon from Madagascar are known from the larval stage, we expect that the setation of the labrum (Fig. \), abdominal color pattern, tergal armature (Fig. 9), and paraproctal spination (Fig. 10) will be diagnostic features of C. emmanueli. The genus Cloeon has been reported from much of the world, although Vol. 109, No. 5, November & December, 1998 359 reports of its presence in South America are considered incorrect (McCafferty 1998), and it is only adventive in North America (McCafferty 1996). Its pres- ence in southern Africa and the Orient suggested that it could be present in Madagascar. However, species previously assigned to Cloeon in Madagascar include only the highly dubious C. durani Navas (1926), C. cambouei Navas (1930), and C. irretitum Navas (1936). Cloeon durani and C. cambouei were originally described from female subimagos, and C. irretitum was originally 8 9 10 Figs. 1-10. Cloeon emmanueli, larva. 1. Labrum (dorsal). 2. Hypopharynx. 3. Left mandible. 4. Right mandible. 5. Right maxilla. 6. Labium (right- ventral; left-dorsal). 7. Right foreleg. 8. Tarsal claw. 9. Tergum 4 (detail). 10. Paraproct. 360 ENTOMOLOGICAL NEWS described from male and female subimagos. Because the three species are so poorly known and because their taxonomic status cannot be corroborated, we place the three names as nomina dubia. Thus, C. emmanueli is the only bona fide species of Cloeon known from Madagascar at this time. Demoulinia insularis Lugo-Ortiz and McCafferty, NEW SPECIES Larva. Body length: 7.3 mm. Caudal filaments length: 3.5 mm. Head: Coloration me- dium yellow-brown, with no distinct pattern. Antennal length unknown. Labrum (Fig. 1 1 ) with numerous long, fine, simple setae scattered over surface. Hypopharynx as in Figure 12. Left mandible (Fig. 13) with outer set of incisors with three denticles and inner set with two den- ticles. Right mandible (Fig. 14) with outer set of incisors with three denticles and inner set with two denticles. Maxillae (Fig. 15) with row of 10-12 long, fine, simple setae near crown of galealaciniae and row of six to seven long, fine, simple setae submedially in midregion; palps two segmented; palp segment 1 approximately 0.70x length of segment 2. Labium (Fig. 16) with glossae subequal in length to paraglossae; glossae broadly rounded apically, with minute, stout, simple setae dorsally; paraglossae acute apically, with numerous long, fine, simple setae ventrally and three rows of long, fine, simple setae dorsally; palp segment 1 approximately O.SOx length of segments 2 and 3 combined, with numerous long, fine, simple setae basomedially; segment 2 approximately 2.25x length of segment 3, with numerous long, robust, simple setae on distomedial process; segment 3 slender and elongate, with numerous long, robust, simple setae scattered over surface. Thorax: Coloration medium yellow-brown, with no distinct mark- ings. Hindwingpads absent. Legs (Fig. 17) pale yellow-brown; femora with numerous minute, fine, simple setae and minute, stout, simple setae dorsally and ventrally, setae more abundant ventrally; tibiae and tarsi with numerous minute, fine, simple setae dorsally and numerous minute, stout, simple setae ventrally; tarsal claws approximately 0.63x length of tarsi, eden- tate. Abdomen: Coloration medium brown to yellow-brown; tergum 1 medium brown, with no markings; tergum 2 medium brown, with anteromedial pair of round, medium brown spots; terga 3-9 medium brown anteriorly, yellow-brown posteriorly, with anteromedial pair of me- dium brown dashes; terga 7-9 with submedial pair of round, medium brown spots in midregion; tergum 10 yellow-brown. Sterna pale yellow brown, with no distinct pattern. Terga (Fig. 18) with numerous scale bases; posterior triangular spines approximately 1.2x basal width. Gills subtriangular, poorly tracheated, marginally smooth. Paraproct (Fig. 19) with numerous mar- ginal spines, increasing in size apically. Caudal filaments pale yellow-brown; terminal fila- ment subequal in length to cerci. Adult. Unknown. Material examined. Holotype: Larva, MADAGASCAR, Antananarivo (= Tananarive) Prov., Ankeniheny R., 28°C, 4 km S of Manjakatompo Forest Station, 1 -XI- 1 97 1 , G.F., C. H. Edmunds, and F. Emmanuel [mouthparts, forelegs, tergum 4, and paraproct on slide (medium: Euparal)]. Etymology. The specific epithet is a Latin word meaning "from an is- land." Discussion. Demoulinia has been known previously only from South Af- rica (Demoulin 1 970, Gillies 1 990). Demoulinia insularis is distinguished from the southern African species D. crassi (Demoulin) by the relatively wide anteromedial emargination of the labrum (Fig. 11), edentate tarsal claws (Fig. 17), and numerous small spines of the paraproct (Fig. 19). Vol. 109, No. 5, November & December, 1998 361 The presence of D. insularis in Madagascar is significant because it indi- cates that Demoulinia was well established in at least West Gondwanaland before the island began to separate from the African landmass approximately 100 million years ago. 11 12 13 15 16 17 \rwv~www 18 19 Figs. 1 1 -19. Demoulinia insularis, larva. 1 1. Labrum (dorsal). 12. Hypopharynx. 13. Left man- dible. 14. Right mandible. 15. Right maxilla. 16. Labium (left-ventral; right-dorsal). 17. Right foreleg. 18. Tergum 4 (detail). 19. Paraproct. ACKNOWLEDGMENTS We thank G. F. Edmunds, Jr. (Salt Lake City, Utah) for the donation of the material used in this study. 362 ENTOMOLOGICAL NEWS LITERATURE CITED Demoulin, G. 1970. Ephemeroptera des faunes ethiopienne et malgache. S. Afr. Anim. Life 14: 24-170. Demoulin, G. 1973. Ephemeropteres de Madagascar. III. Bull. Inst. R. Sci. Nat. Belg. 49: 1- 20. Gillies, M. T. 1990. A revision of the African species of Centroptilum Eaton (Baetidae, Ephemeroptera). Aq. Insects 12: 97-128. Lugo-Ortiz, C. R. and W. P. McCafferty. 1996a. The Bugilliesia complex of African Baetidae (Ephemeroptera). Trans. Am. Entomol. Soc. 122: 175-198. Lugo-Ortiz, C. R. and W. P. McCafferty. 1996b. Crassabwa: a new genus of small minnow mayflies (Ephemeroptera: Baetidae) from Africa. Ann. Limnol. 32: 235-240. Lugo-Ortiz, C. R. and W. P. McCafferty. 1996c. The composition of Dabulamanzia, a new genus of Afrotropical Baetidae (Ephemeroptera), with descriptions of two new species. Bull. Soc. Hist. Nat. Toulouse 132: 7-13. Lugo-Ortiz, C. R. and W. P. McCafferty. 1997a. Edmulmeatus grandis: an extraordinary new genus and species of Baetidae (Insecta: Ephemeroptera) from Madagascar. Ann. Limnol. 33: 191-195. Lugo-Ortiz, C. R. and W. P. McCafferty. 1997b. Labiobaetis Novikova & Kluge (Ephemeroptera: Baetidae) from the Afrotropical region. Afr. Entomol. 5: 241-260. Lugo-Ortiz, C. R. and W. P. McCafferty. 1 997c. Contribution to the systematics of the genus Cheleocloeon (Ephemeroptera: Baetidae). Entomol. News 108: 283-289. Lugo-Ortiz, C. R. and W. P. McCafferty. 1997d. Maliqua: a new genus of Baetidae (Ephemeroptera) for a species previously assigned to Afroptilum. Entomol. News 108: 367- 371. Lugo-Ortiz, C. R. and W. P. McCafferty. 1997e. New Afrotropical genus of Baetidae (In- secta: Ephemeroptera) with bladelike mandibles. Bull. Soc. Hist. Nat. Toulouse 133: 41- 46. Lugo-Ortiz, C. R. and W. P. McCafferty. 1997f. New species and first reports of the genera Cheleocloeon, Dabulamanzia, and Mutelocloeon (Insecta: Ephemeroptera: Baetidae) from Madagascar. Bull. Soc. Hist. Nat. Toulouse 133: 147-53. Lugo-Ortiz, C. R. and W. P. McCafferty. 1998a. The Centroptiloides complex of Afrotropical small minnow mayflies (Ephemeroptera: Baetidae). Ann. Entomol. Soc. Am. 91: 1-26. Lugo-Ortiz, C. R. and W. P. McCafferty. 1998b. Cheleocloeon falcatum (Crass): a new combination for a southern African species previously assigned to Afroptilum Gillies (Ephemeroptera: Baetidae). Afr. Entomol., in press. McCafferty, W. P. 1996. The Ephemeroptera species of North America and index to their complete nomenclature. Trans. Am. Entomol. Soc. 122: 1-54. McCafferty, W. P. 1 998. Ephemeroptera and the great American interchange. J. N. Am. Benthol. Soc., 17: 1-20. McCafferty, W. P., C. R. Lugo-Ortiz, and H. M. Barber-James. 1997. Micksiops, a new genus of small minnow mayflies (Ephemeroptera: Baetidae) from Africa. Entomol. News 108: 362-366. Navas, L. 1926. Algunos insectos del Museo de Paris (3a serie). Broteria Zool. 23: 95-1 15. Navas, L. 1930. Insectos del Museo de Paris (5a serie). Broteria Zool. 24: 5-24. Navas, L. 1936. Comunicaciones entomologicas. 19. Insectos de Madagascar. Rev. Acad. Ci. Exac. Fisquim. Nat. Zaragoza 19: 100-1 10. Wuillot, J. and M. T. Gillies. 1994. Dicentroptilum, a new genus of mayflies (Baetidae, Ephemeroptera) from Africa. Aq. Insects 16: 133-140. Vol. 109, No. 5, November & December, 1998 363 AEDES ALBOPICTUS (DIPTERA: CULICIDAE) OCCURRENCE THROUGHOUT TENNESSEE, WITH BIOLOGICAL NOTES1 James P. Moore^ ABSTRACT: Aedes albopictus, the Asian tiger mosquito, was recorded for the first time in 87 of Tennessee's 95 counties. Continued occurrence of this species in the remaining 8 Tennessee counties was confirmed. Notes are provided on other mosquito species sharing larval habitats with Ae. albopictus, as well as the observed photoperiod- induced egg diapause of Ae. albopictus in Montgomery County, Tennessee. A survey of Tennessee's 95 counties was conducted during 1997 to deter- mine the presence of Aedes albopictus (Skuse), the Asian tiger mosquito. This survey, primarily of water-containing tire habitats, established the first record of Ae. albopictus in 87 Tennessee counties and confirmed the continued pres- ence of the species in the remaining 8 counties with previously reported infes- tations (Chet Moore, CDC, personal communication). These 8 counties are Anderson, Coffee, Davidson, Gibson, Henderson, Lawrence, Montgomery, and Shelby. Mosquito larvae were collected from 108 sites in 86 counties from July to October 1997. These sites consisted of new and waste automotive tires located at roadside dumps (12), county waste collection points (2), and commercial tire businesses (91). Other sites included outdoor plastic and masonry contain- ers at residences (2) and roadside dumps (1). Larvae were collected directly from the tire or other container using a siphon, transported to the laboratory, and identified using standard light microscopy and the taxonomic references of Darsie and Ward ( 1 981), Darsie (1986), and Reinertetal. (1997). At 51% of the larval collection sites, Ae. albopictus was the only culicid species collected (Table 1 ). Aedes albopictus shared the habitat with other mosquito species at an additional 31% of the sites, making this species the predominant culicid resident of tire habitats in Tennessee. Adult Ae. albopictus were collected from 50 sites in 50 counties during this survey. An aspirator was used to collect the adults (both sexes) resting at 26 sites and feeding on man at 24 sites. Adult specimens were examined using a dissecting microscope and identified using the taxonomic references of Darsie and Ward ( 1 98 1 ) and Darsie ( 1 986). Collection data for each collection site is preserved and available for anyone wishing to visit the collection sites for control purposes. Aedes triseriatus (Say) was found as the sole inhabitant at 5% of the sites. 1 Received February 6, 1998. Accepted April 19. 1998. 2 The Center for Field Biology, Austin Peay State University, Clarksville, TN 37044. ENT. NEWS 109(5): 363-365, November & December, 1998 364 ENTOMOLOGICAL NEWS Aedes triseriatus was found with other culicid species at an additional 6% of the sites; Ae. albopictus was always one of its cohabitants. This indicates that the Asian tiger mosquito has not totally excluded Ae. triseriatus from tire habi- tats in Tennessee, as had been predicted by Livdahl and Willey (1991). No specimens of Aedes aegypti (Linnaeus) were collected during this sur- vey. However, Ae. aegypti larvae were collected previously by the author from tire habitats in Montgomery County, TN, during July 1996 and April 1997. This may indicate that Ae. albopictus is competitively displacing Ae. aegypti from tire habitats in Tennessee. However, most of Tennessee is in the region identified by Darsie and Ward (1981) as the extreme range for Ae. aegypti. Some populations of Ae. albopictus exhibit a photoperiod-induced egg diapause (Hawley et al. 1989, Estrada-Franco and Craig 1995). As the species adapts to new locations, the photoperiod response may be altered, providing researchers with a measure of the length of time the species has inhabited a region (Chet Moore, CDC, personal communication). There are no historical data on the photoperiod ism of Ae. albopictus in Tennessee. In Montgomery County, TN, during 1997, the hatching of Ae. albopictus eggs in outdoor arti- ficial containers was observed as early as 1 7 April (13.2 hours daylight) and as late as 2 October (1 1.8 hours daylight). Table 1. Species composition of larval mosquito collections from Tennessee (July - October 1997), showing percentage of 108 collection sites with a species alone (Solo) and percentage of collection sites with cohabitant culicid species (With others). Percentage of 108 larval collection sites Mosquito species Solo With others Total % Aedes albopictus (Skuse) 51 31 82 Aedes atropalpus (Coquillett) 2 24 Aedes triseriatus (Say) 5 6 11 Anopheles punctipennis (Say) / perplexens Ludlow 0 1 1 Anopheles quadrimaculatus sensu stricto Say 0 1 1 Culex pipiens Linnaeus/ quinquefasciatus Say 6 11 17 Culex restuans Theobald 5 7 12 Culex territans Walker 0 22 Toxorhynchites rutilus septentrionalis (Dyar & Knab) 3 47 Vol. 109, No. 5, November & December, 1998 365 ACKNOWLEDGMENTS Grateful appreciation is extended to The Center for Field Biology and the Department of Biology, Austin Peay State University, Clarksville, TN, for their support and laboratory facili- ties, and to Chet Moore, Division of Vector-Borne Infectious Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Fort Collins, CO, for his ad- vice and encouragement; and to Steve Hamilton, Department of Biology, Austin Peay State University, Clarksville, TN, and C. Steven Murphree, Department of Biology, Belmont Univer- sity, Nashville, TN, for providing helpful suggestions with the manuscript. LITERATURE CITED Darsie, R.F., Jr. 1986. The identification of Aedes albopicius in the Nearctic Region. J. Am. Mosq. Control Assoc. 2(3): 336-40. Darsie, R.F., Jr., and R.A. Ward. 1981. Identification and geographical distribution of the mosquitoes of North America, north of Mexico. Mosq. Syst. Suppl. 1, 313 pp. Estrada-Franco, J.G., and G.B. Craig, Jr. 1995. Biology, disease relationships, and control of Aedes albopictus; Pan Amer. Health Org. Tech. Paper No. 42. Wash., DC, 49 pp. Hawley, W.A., C.B. Pumpuni, R.H. Brady, and G.B. Craig, Jr. 1989. Overwintering survival of Aedes albopictus (Diptera: Culicidae) eggs in Indiana. J. Med. Entomol. 26(2): 122-9. Livdahl, T.P., and M.S. Willey. 1991. Prospects for an invasion: competition between Aedes albopictus and native Aedes triseriatus. Science 253: 189-91. Reinert, J.F., P.E. Kaiser, and J.A. Seawright. 1997. Analysis of the Anopheles (Anopheles) quadrimaculatus complex of sibling species (Diptera: Culicidae) using morphological, cy- tological, molecular, genetic, biochemical, and ecological techniques in an integrated ap- proach. J. Am Mosq. Control Assoc. 13(Suppl.): 1-102. 366 ENTOMOLOGICAL NEWS DISTRIBUTION AND HABITAT OF CURICTA PRONOTATA (HEMIPTERA: NEPIDAE) IN SOUTHEASTERN ARIZONA1 Jon D. Hoekstra2, Robert L. Smith3 ABSTRACT: Curicta pronotata is known from western Mexico and southeastern Arizona. Pre- vious accounts of the species' Arizona distribution draw from only a few collections and locali- ties. Distributional records clarifying the range and habitat of C. pronotata in Arizona are re- ported here. The genus Curicta (Hemiptera: Nepidae) is primarily Neotropical with spe- cies entering the United States in Arizona, Texas, and Louisiana (Menke, 1 979). Curicta pronotata 's principal range is in Mexico along the Sierra Madre Occi- dental; it extends from Nayarit north to eastern Sonora and western Chihuahua (Keffer 1996). C. pronotata has previously been reported from two different canyons in the Huachuca Mts. in southern Arizona (Dubois 1 978, Keffer 1 996), and Sites and Polhemus (1994) reported the species from Sabino Canyon near Tucson on the basis of an individual collected in 1937 (full record given here). This report extends the range of the species in Arizona. Curicta pronotata has now been recorded from the Huachuca, Santa Catalina, and Galiuro moun- tain ranges in southern Arizona. Based on our own findings and habitat infor- mation from previous records, the habitat of C. pronotata in Arizona appears to be small shaded streams with woody debris above about 1200m. NEW RECORDS Hemiptera: Nepidae: Curicta pronotata Kuitert 1949. Arizona: Pima County: 1 male, Santa Catalina Mts., Sabino Canyon, 29- VII- 1937, E.D. Ball, UAIC; Pima County: 1 nymph, Santa Catalina Mts., Sabino Canyon, 3660' (11 15 m), 32°22'00" N, 110°47'10" W, 6-VI-1997, J.D. Hoekstra, UAIC; Pima County: Santa Catalina Mts., Bear Canyon, 5530' (1685 m), 32°2r45" N, 110°42'30" W, 19- VII- 1997, J.D. Hoekstra and C. Creighton, 1 male, 1 female, 12 nymphs, UAIC, 1 female, 5 nymphs, JTPC; Cochise County: 1 female, Galiuro Mts., Wildcat Canyon, 4120' (1255 m), 32°22'00" N, 110°15'30" W, 20-VIII- 1996, J.D. Hoekstra and D.A. Lytle, JTPC; Cochise County, 1 male, same locality data as preceding, 21 -IX- 1996, J.D. Hoekstra, JTPC. Abbreviations: JTPC = J. T. Polhemus Collection, Englewood, CO. UAIC = University of Ari- zona Insect Collection, Tucson, AZ. DISCUSSION In Sabino Canyon, a single nymph was collected from mud under a rock 1 Received December 29, 1997. Accepted February 8, 1998. •^ L Center for Aquatic Ecology, Illinois Natural History Survey, 607 E. Peabody Dr., Champaign, IL61820. -* Department of Entomology, University of Arizona, Tucson, AZ 85721. ENT. NEWS 109(5): 366-368, November & December, 1998 Vol. 109, No. 5, November & December, 1998 367 along the margin of a drying pool. The nymph was inactive and presumed dead until it was placed in ethanol, whereupon it revived for a short time. The nymph's behavior may have been an example of "death-feigning" (thanatosis) as reported in Nepa and Ranatra, or it may have indicated the initiation of estivation, which has been documented in Nepa (Sites and Polhemus 1994). This nymph is the first evidence of C. pronotata in Sabino Canyon since it was collected in 1937. The authors have sampled the habitat in which it was found extensively over two seasons and have found only this single nymph. We sus- pect that it was a "stray" which had drifted down from an upstream source population in Sabino Canyon or a tributary. In Bear Canyon, Santa Catalina Mts., adults and especially nymphs were very abundant among woody detritus in shallow bedrock-lined pools. The stream was narrow (less than 1 m wide) and shallow (average maximum depth 30 - 40 cm). Curicta pronotata shared this habitat with Abedus herberti (Hemi- ptera: Belostomatidae), which was also abundant. Of the twenty C. pronotata collected on July 19, 3 were adults, 10 were F-l instar nymphs, and 7 were F- 2 instar nymphs. In Curicta scorpio, the fourth and fifth stadia lasted an aver- age of 18.56 and 18.87 days for artificially reared individuals from two popu- lations in Texas (Keffer et al. 1 994). If C. pronotata has a similar developmen- tal rate, most of the individuals in the Bear Canyon population probably eclosed to the adult stage by September. Arizona collections of Curicta pronotata have been very few, despite the species' fairly wide range as indicated by the records reported here. The spe- cies probably has been overlooked because of its cryptic appearance and ten- dency to feign death when captured (Sites and Polhemus 1994). In addition, spatial and temporal components of the species' occurrence in Arizona may have contributed to its rarity in collections. Arizona populations of C. pronotata appear to be highly localized. Such local populations could be relicts of a previously continuous distribution. The Sonoran Desert region has aridified over the past 11,000 yr., with attendant restriction of previously widespread mesic biotic communities to high eleva- tions (Hall et al., 1989). This process may have reduced the number and ex- tent of suitable habitats for C. pronotata, such that it currently persists in only a few favorable habitat refuges. Densities of C. pronotata probably fluctuate seasonally with changes in streamflow, as noted by Keffer ( 1 996) for Texas populations of Curicta scorpio. Local populations of C. pronotata may also be unstable from year to year in Arizona. The species is probably capable of dispersal by flight, which has been reported for C. scorpio (Sites and Polhemus 1994). Thus the disjunct Arizona populations could be transient "sink" populations in a metapopulation with a "core" to the south in the Sierra Madre Occidental. Such a metapopulation dynamic has been reported for several southeastern Arizona butterfly species 368 ENTOMOLOGICAL NEWS (Bailowitz and Brock 1991). Additional surveys and long term monitoring of the Arizona populations will be required to evaluate these alternative hypotheses about the population dynamics and biogeography of C. pronotata at the northern limits of its range. ACKNOWLEDGMENTS We thank J.T. Polhemus for making the specific determinations on individuals from the populations reported. J.D. Hoekstra also thanks the UA Department of Entomology and the ARCS (Acheivement Rewards for College Scientists) Foundation for support during the time in which this research was conducted. LITERATURE CITED Bailowitz, R.A. and J.P. Brock. 1991. Butterflies of Southeastern Arizona. Sonoran Arthropod Studies, Inc., Tucson, Arizona. DuBois, Mark B. 1978. A recent record of Curicla pronotata Kuitert (Hemiptera:Nepidae) from its type locality. Entomol. News 89: 207. Hall, W.E., C.A. Olson, and T.R. VanDevender. 1 989. Late Quaternary and Modern Arthropods from the Ajo Mountains of southwestern Arizona. Pan-Pacific Ent. 65: 322-347. Keffer, S.L. 1996. Systematics of the New World waterscorpion genus Curicla Stal (Heteroptera: Nepidae). J. New York Entomol. Soc. 104(3-4): 117-215. Keffer, S.L. 1991. Taxonomic revision of the neotropical genus Curicta Stal (Insecta: Heteroptera: Nepidae). Ph.D. dissertation, S. I. U., Carbondale. Keffer, S.L., SJ. Taylor and J.E. McPherson. 1994. Laboratory rearing and descriptions of immature stages of Curicta scorpio (Heteroptera: Nepidae). Ann. Entomol. Soc. Am. 87( 1 ): 17-26. Kuitert, Louis C. 1949. Some new species of Nepidae (Hemiptera). J. Kansas Entomol. Soc. 22: 60 - 68. Menke, A.S. 1979. Family Nepidae. Pp. 70-75 in A.S. Menke [ed.],The semiaquatic and aquatic Hemiptera of California (Heteroptera: Hemiptera). Bull. Calif. Ins. Surv. 21: 1-166. Polhemus, J.T. 1976. Notes on the North American Nepidae. Pan-Pacific Ent. 52: 204-208. Sites, R.W. and J.T. Polhemus. 1994. Nepidae (Hemiptera) of the United States and Canada. Ann. Entomol. Soc. Am. 87( 1 ): 27-42. Vol. 109, No. 5, November & December, 1998 369 HEXAMETH YLDISILAZANE - A CHEMICAL ALTERNATIVE FOR DRYING INSECTS1 John Heraty, David Hawks^ ABSTRACT: Two methods of chemically drying softbodied Chalcidoidea (Hymenoptera) are compared: critical-point drying (CPD) and hexamethyldisilazane (HMDS). For three groups of Eulophidae, Encyrtidae and miscellaneous Chalcidoidea, the CPD specimens were of consis- tently higher quality for all groups, although the overall differences between CPD and HMDS specimens were marginal. Soft-bodied insect specimens have long been the bane of systematics. Freshly killed and air-dried specimens (Fig. 1 ) undergo partial to complete collapse of body parts, whereas specimens initially preserved in EtOH fare even worse when subsequently removed from the liquid and air dried (Fig. 2). This is not only a problem of obtaining quality museum specimens but in the past has deterred some systematists from bothering with samples preserved in alcohol, such as those taken in malaise or pan traps. Critical-point drying (CPD) of specimens through a liquid CO2 intermediate (Gordh & Hall 1979) pro- vides a means of retrieving large numbers of soft-bodied specimens from EtOH and is being widely used for some taxa, especially Chalcidoidea. The primary advantage of using CPD is little or no collapse of soft body parts, including internal muscles and nerves. Secondarily, the structure of muscles, nerve tis- sue and other internal body parts is maintained, allowing for later survey of these structures from museum specimens (Heraty et al. 1997). The disadvan- tages with the CPD are that it 1 ) is relatively expensive to buy the initial equip- ment ($2,000-8,000), 2) is necessary to obtain specialized CO2 tanks that must be maintained above 900 psi, 3) is labor intensive, 4) can cause abnormal swelling or occasional bursting of some body parts, and 5) may leave surface residues on specimens. Several alternatives to air drying or CPD have been proposed, some of which are freeze drying, Peldri II (Brown 1990), acetone vapor (van Noort 1995), xylene (R. Carlson pers. comm.), and hexane (D. Hawks, pers. comm.). A new chemical method involving hexamethyldisilazane (HMDS) has been proposed as a simple and cost-effective means of retrieving high-quality speci- mens from collections preserved in EtOH (Nation 1983, Brown 1993). Only the CPD and HMDS methods are regularly applied for the retrieval of large collections of Chalcidoidea initially preserved in alcohol, and here we com- pare the two methods. METHODS All specimens were initially killed and preserved in 70-75% EtOH at 4°C. 1 Received October 22, 1997. Accepted March 31, 1998. 2 Department of Entomology, University of California, Riverside, CA 92521. ENT. NEWS 109(5): 369-374, November & December, 1998 370 ENTOMOLOGICAL NEWS Evaluations were of separate collections made from 1990 to 1996 in southeast Asia, the Galapagos Islands and California. Lots that had a high proportion of soft-bodied Chalcidoidea were chosen, and all specimens were scored from each lot. Fourteen separate collections (362 specimens) were evaluated for the CPD method and 5 collections (347 specimens) for HMDS. Overlap in collec- tion time and country for each method occurred only for the southeast Asian collections. Additional specimens of a new species ofdrrospilus (Eulophidae) from California were examined as representatives of very soft-bodied Chal- cidoidea. Specimens were scored on a scale of 1 to 5, with 5 being a nearly perfect specimen suitable for scanning electron microscopy (SEM). Scores were based only on the softer body parts. Cirrospilus are almost entirely soft-bodied and represent an extreme; in other taxa, for example pteromalids, the head and mesosoma are well-sclerotized and do not collapse under any treatment, but the antennae and gaster will partially or completely collapse. A score of 1 would be typical of air-dried eulophids taken from alcohol: completely shriv- elled and collapsed (Fig. 2). A score of 2 was assigned to specimens that had extensive collapse of the softer body structures (head, antennae and gaster) (Fig. 1 ). A score of 3 was given to specimens with partial collapse of all softer body parts (Figs 4, 5). Freshly killed and air-dried specimens would usually be given a score between 1 and 3, with a score of 3 bordering on acceptable for museum collections or SEM (at least partly shrivelled or collapsed). A score of 4 was given for very minimal collapse of not more than one body part or a slight distortion (wrinkling or bloating) of the gaster (Figs 4, 5). The Cirrospilus were not scored for comparative analysis. All material is deposited in the En- tomology Research Museum, University of California, Riverside. CPD method. The liquid vapor interface is the primary destructive force in air-drying specimens, and if not about equal, results in the breakdown of cell walls and collapse of tissue. For CO2 the identical vapor pressure as a liquid or gas, the critical point, is reached at 3 1 .0°C and 1093 psi (Burstyn and Bartlett 1975). Specimens were dried as outlined by Gordh & Hall (1979) by 1) dehydrating the specimens to 100% EtOH, 2) exchanging fluids through liquid CO2 under high pressure (900 psi) and low temperature (11-15°C) through a series of soaks and purges until the exhausted dry CO2 did not leave a liquid residue (complete sublimation), 3) drying under high pressure (1 100- 1200 psi) until the chamber temperature reached 41-43°C, and then 4) slowly exhausting the gaseous CO2 to room atmospheric pressure. HMDS method. HMDS ([(CH3)3Si]2NH) reacts with water to produce hexamethyldisiloxane ([(CH3)3Si]2O) and ammonia (NH3), both of which evaporate from the specimen (Dave Jordon, Polysciences Inc., pers. comm.). Specimens were dried in the manner outlined by Brown (1993) by 1) dehy- drating the specimens to 100% EtOH, 2) replacing the alcohol with HMDS for two soaks of 1/2 hour each in a covered glass vial or dish, and 3) after the Vol. 109, No. 5, November & December, 1998 371 second soak, pouring off most of the HMDS and allowing the remaining HMDS to evaporate in a fume hood (or outdoors in a well-ventilated area). Samples can be soaked and dried in glass vials or dishes. Gas buildup in the vials may cause the release of liquid HMDS while being uncapped, but this can be avoided by using smaller volumes of HMDS (less than half of vial) or slowly unscrew- ing the vial top. We prefer to line the bottom of a glass dish with a fine brass screen and cover each sample with a screen lid during evaporation, thus pre- venting dried specimens from disappearing into the exhaust. HMDS is a skin irritant, and gloves and eye protection are recommended. All specimens were card-mounted for examination following Noyes ( 1 982). RESULTS Cirrospilus (Eulophidae) was used as an example of a very soft-bodied species that does not fare well under any of the drying methods (Figs 1 -6). Air- drying (Fig. 1) resulted in collapse of the antennae, femora, and gaster dor- Figures 1-6. Cirrospilus sp. (Eulophidae): 1, freezer killed and air dried. 2-6, killed and pre- served in 70% EtOH and then: 2, air-dried; 3&5, CPD dried; 4&6, HMDS dried. 372 ENTOMOLOGICAL NEWS sally and laterally; the mesosoma was relatively undistorted. The specimen illustrated would receive a score of 2, which would be marginally acceptable for use in collections. Air-drying from alcohol (Fig. 2) was disastrous, with general collapse of all body parts (score 1). CPD Cirrospilus (Figs 3, 5) showed slight collapse of the scape and scrobes, and distortion but not collapse of the gastral tergites. Such a specimen (Fig. 3) would be scored as a 4 (less than perfect). HMDS Cirrospilus (Fig. 4, 6) exhibited a greater degree of collapse of the scape, head and metasoma, with the specimen receiving a score of 3. For extremely soft-bodied specimens, the CPD method was consistently bet- ter than the HMDS method, and both were better than air-drying. Seven families of Chalcidoidea were encountered in the 19 collections evaluated (Table 1). Each family presents a different problem with respect to how they were affected by improper drying. Even when CPD- or HMDS- treated, soft-bodied Eulophidae generally had some collapse or distortion of all body parts (cf. Figs. 3-5). Using either CPD or HMDS, 51% of the Table 1. Quality of soft-bodied Chalcidoidea dried using critical-point drying (CPD) or hexamethyldisilazane (HMDS). Ranking based on a scale of 1 -5, with 5 indicating a near-perfect specimen. Mean values were significantly higher for CPD specimens for all groups (Chi-square, P=0.01). Data were pooled for Aphelinidae, Mymaridae, Pteromalidae, Torymidae and Trichogrammatidae. rank 5 4 3 2 1 n mean rank Eulophidae CPD 126 114 7 0 0 247 4.48 HMDS 87 60 22 0 0 169 4.38 Encyrtidae CPD 46 19 6 0 0 71 4.56 HMDS 29 32 0 0 0 61 4.48 Aphelinidae CPD 7 2 0 0 0 """ 44 4.82 HMDS 56 2 2 2 0 117 4.51 Pteromalidae CPD 7 4 1 0 0 HMDS 13 9 9 1 0 Mymaridae CPD 4 4 1 0 0 HMDS 6 6 0 0 1 Trichogrammatidae CPD 11 1 1 0 0 HMDS 8 2 0 0 0 Torymidae CPD 1 0 0 0 0 HMDS — — — — — ^ Vol. 109, No. 5, November & December, 1998 373 Eulophidae treated had a score of 5, and, although a much higher proportion than the CPD method, only 13.0% of the specimens received a score of 3, and none received a 1 or 2. Many Eulophidae are reasonably well-sclerotized and do not have problems similar to those of Cirrospilus. Often the most notice- able artifact was a slight wrinkling of the gastral tergites (score of 4), which was common in both treatments. Pteromalidae generally have a well-sclero- tized head and mesosoma, but the gaster of males is particularly susceptible to collapse. Both Trichogrammatidae and Aphelinidae are soft-bodied but re- sponded well to either technique except for some collapse of the antennae, which occurred with use of either method. Other than Eulophidae, all of the chalcidoid groups responded well to either technique, with consistent scores of 4 or 5, both of which are acceptable for museum collections. For statistical comparisons, Eulophidae and Encyrtidae were common in all samples and were treated separately; results for Aphelinidae, Pteromalidae, Mymaridae, Torymidae and Trichogrammatidae were pooled. In all three com- parisons, the CPD specimens were of significantly higher quality (rank) than the HMDS specimens (Chi Square, P=0.01), although the differences in the mean rank scores for each treatment were marginal (Table 1 ). The CPD method after ethanol fixation also ranked better than HMDS in a study of pre- and post-fixation techniques in four taxa (Swearingen et al. 1997). In contrast to the techniques used by Swearingen et al. (1997), we have not found fixation in osmium tetroxide to be a necessary step in preparation for either museum or SEM specimens. CPD and HMDS methods left little or no residue on the specimens, as noted by Swearingen et al. (1997). Specimens treated by HMDS appeared to be slightly cleaner, but we could see no way to quantify this characteristic accurately. HMDS also works as a good degreasing agent for some insects such as tiger beetles and robber flies. We also found various labels and ink types (including laser-printed labels) to be unaffected by HMDS, allowing their inclusion during processing. The same is possible for the CPD method, although processing is usually in small capsules making inclusion of larger labels impossible. At $30 U.S. per 400 ml of HMDS and 5 ml per large lot of about 100 chalcidoids, we estimate a cost of about 37.5 cents per run, or 0.4 cents per specimen. We have tried HMDS on a variety of insects, including Collembola, flies, beetles and other Hymenoptera (Perdita and Bombus), with generally excellent results. Heavily sclerotized individuals processed using HMDS are as good as CPD specimens. Internally, muscles and nerve tissue are preserved in the same manner as using the CPD process. For larger speci- mens, wings are often crumpled in smaller CPD capsules, but this was not a factor with HMDS. In addition to improved specimen quality, it is also note- worthy that mitochondrial DNA was successfully extracted from dried CPD and HMDS specimens of Ichneumonidae and Encyrtidae (Austin & Dillon 1997). 374 ENTOMOLOGICAL NEWS In summary, the use of HMDS is a viable alternative to use of CPD for retrieving soft-bodied insects from alcohol. CPD specimens are marginally better in quality than those treated with HMDS, but HMDS is cost-effective and less labor intensive than CPD. If the equipment is not available, HMDS may be the preferred technique. ACKNOWLEDGMENTS John Pinto, Gary Plainer and Serguei Triapitsyn (University of California, Riverside) re- viewed an earlier draft of this manuscript. LITERATURE CITED Austin, A. D. and N. Dillon. 1 997. Extraction and PCR of DNA from parasitoid wasps that have been chemically dried. Aust. J. Entomol. 36: 241-244. Brown, B.V. 1990. Using Peldri II as an alternative to critical point drying for small flies. Fly Times 4: 6. Burstyn, H.P. and A.A. Bartlett. 1975. Critical point drying: application of the physics of the PVT surface to electron microscopy. Am. J. Physics 43: 414-419. Brown, B.V. 1993. A further chemical alternative to critical-point-drying for preparing small (or large) flies. Fly Times 7: 10. Cowan, D. 1995. Another method of drying chalcidoids. Chalcid Forum 18: 4-5. Gordh, G. and J. Hall. 1979. A critical point drier used as a method of mounting insects from alcohol. Entomol. News 90: 57-59. Noyes, J.S. 1 982. Collecting and preserving chalcid wasps (Hymenoptera: Chalcidoidea). J. Nat. Hist. 16: 315-334. Swearingen, M., D. Headrick and T. Bellows 1997. Comparison of fixation and drying proce- dures for scanning electron microscopy among insect body types. Proc. Entomol. Soc. Wash. 99: 513-522. van Noort, S. 1995. A simple yet effective method for drying alcohol preserved specimens. Chalcid Forum (newsletter) 18: 3-4. Vol. 109, No. 5, November & December, 1998 375 STATEMENT OF OWNERSHIP, MANAGEMENT & CIRCULATION 1. Title of publication: ENTOMOLOGICAL NEWS 2. Date of filing October 1, 1998. 3. Frequency of issue: Bimonthly (every other month) except July and August 4. Location of known office of publication: 232 Oak Shade Rd., Tabernacle Twp., Vincentown PO, New Jersey 08088 5. Location of the headquarters or general business offices of the publishers: 1900 Benjamin Franklin Parkway, Philadelphia, PA 19103-1 195 6. Name and address of publisher, editor and managing editor: Publisher: American Entomological Society, 1900 Benjamin Franklin Parkway, Philadelphia. PA. 19103-1 195. Editor; Howard P. Boyd, 232 Oak Shade Rd Tabernacle Twp, Vincentown PO, New Jersey, 08088 7. Owner: American Entomological Society, 1900 Benjamin Franklin Parkway, Philadelphia. PA 19103-1 195 8. Known bondholders, mortgagees and other security holders owning or holding one percent or more of total amount of bonds, mortgages and other securities: None 9. For optional completion by publishers mailing at the regular rates: signed 10. For completion by nonprofit organizations authorized to mail at special rates: The purpose, function and nonprofit status of this organization and the exempt status for Federal income tax purposes: Have not changed during preceding 12 months (checked) Average No. 1 1. EXTENT AND NATURE OF CIRCULATION A TOTAL NO. COPIES PRINTED B. PAID CIRCULATION 1 . SALES THROUGH DEALERS AND CARRIERS. STREET VENDORS AND COUNTER SALES 2. MAIL SUBSCRIPTIONS C. TOTAL PAID CIRCULATION D. FREE DISTRIBUTION BY MAIL, CARRIER OR OTHER MEANS. SAMPLES, COMPLI- MENTARY, AND OTHER COPIES E. TOTAL DISTRIBUTION F. OFFICE USE. LEFTOVER. UNACCOUNTED, SPOILED AFTER PRINTING. G. TOTAL Copies Each Issue During Preceding 12 Months 800 0 631 631 0 631 169 800 Actual Number of Copies of Single Issue Published Nearest to Filing Date 800 0 629 629 0 629 171 800 12. I certify that the statements by me above are correct and complete. Signed: Howard P. Boyd, editor. No. 1 2 3 4 5 Date of issue Jan. & Feb. Mar.& Apr. May & June Sept. & Oct. Nov. & Dec. MAILING DATES VOLUME 109. 1998 Pages 1-80 81-152 153-224 225-296 297-380 Mailing Date December 1, 1997 April 14, 1998 June 22, 1998 October 22, 1998 November 30, 1998 376 ENTOMOLOGICAL NEWS Abbot 3tt, J.C., K.W. Stewart Odonata of so. central Nearctic region, incl. northeastern Mexico Acari 172 Acerpenna pygmaea, character varia- 257 bility & new synonym of Aedes albopictus occurrence through- 363 outTenn., with biol. notes Ahlstrom, K.R. 277 Aleiodes depanochora, first record of 213 from Brazil Alford, M.H. 183 New records of Allocapnia from Miss. & La., with electron micrographs Allocapnia, new records of, from Miss. 183 & La., with electron micrographs Amblyomma, ticks of genus, from 172 white-lipped peccaries in north- eastern Bolivia, with comments Amer. Entomol. Soc. membership 219 Amer. Entomol. Soc., 152,224,246 meeting of Ametropus neavei, new distrib. for 213 Andersen, T., J. Huisman 37 A n.sp. of Symphitoneuria from Sabah, Malaysia Announcement 1 52, 200, 276 Aphaenogaster, a n.sp. of, from upland 88 Florida habitats Aradidae 343 Araneida 66 Armitage, B.J. 339 INDEX: Volume 109 201 121, 128,292,295 B aetidae 21,110,117,122,215,257, 261,345,354,357 Baetis adonis & B. caelestis, reared 261 assoc. & equivalency of Baetis complex, key to 345 Barbalho, S.M., A.M. P.-Dias 269 Tax. notes on Evaniodini, with re- descrip. of Evaniodes spathiiformis, & descrip. of a n.sp. Barren, J.A. 20 Baumgardner, D.E. 257 Bickel, D.J. 61 Synonymic notes on some of Thomson's New World Dolichopodidae Book review 164, 254 Books rec'd. & briefly noted Braconidae 217,269,318 Brown, M.W., S.S. Miller 1 43 Coccinellidae in apple orchards in eastern W.VA., & impact of invasion by Harmonia axyridis Bythonia, a n.sp. of Neotropical genus 307 Bythonia consensa, female of 307 ^/ amelobaetidius variabilis, a n.sp. 21 from Tex., Okla, & Mexico Capniidae 183 Carabidae 1 89 Carithers, T.P. 1 98 Carlton, C.E. 1 77 Casamatta, D.A. 256 Cekalovic, K.T. 33 Cerambycidae 75 Ceratopogonidae 282 Cerococcus michaeli, a n.sp. of false 297 pit scale from New Zealand Chen, J.-X., K.A. Christiansen 5 1 Tomocerus spinulus, a n.sp. of Chinese springtail Chloroperlidae 293 Chordas, S.W., III, B.J. Armitage 339 New Ohio records for Corixidae Chorebus, two n.sp. of genus from 3 1 8 Spain Christiansen, K. A. 47,51 Cicadellidae 307 Cicindelidae 165 Cloeodes excogitatus in no. Calif. 215 Cloeodes, first report & n.sp. of 1 22 genus from Australia Cloeon, n.sp. of from Madagascar 357 Coccinellidae 15,143,277 Coleoptera 1 5 , 20, 75 , 1 43 , 1 65 , 1 89 225, 277 Collembola 47,51,195 Corixidae, new Ohio records of 339 Culicidae 363 Curicta pronotata, distrib. & habitat 366 in southeastern Ariz. Vol. 109, No. 5, November & December, 1998 377 D, "asyhelea pseudoincisurata, biol. & 282 morph. notes on Davis, L. 88 Demoulinia, n.sp. of, from Madagascar 357 Dermestidae 20 DeWalt, R.E., B.P. Stark, M. A. Harris 3 1 5 Perlesta golconda, a n.stonefly sp. from Illinois Deyrup, M. 81 Smithistruma memorialis, a n.sp. of ant from Kentucky Cumberland Plateau Deyrup, L. Davis 88 A n.sp. of Aphaenogaster from upland Florida habitats Diplura 177 Diptera 56, 6 1 , 1 59, 282, 325, 363 Docavo, I., J. Tormos 3 1 8 Two n.sp. of Chorebus from Spain Dolichopodidae 61 Dorylinae, first fossil of, with notes 136 Drosophila melanogaster, distinction 56 in abdominal pigmentation patterns, in females, from D. simulans DuBois, M.B. 136 First fossil Dorylinae, with notes on fossil Ecitoninae Duffield, R.M. 36 Dyschirius, first U.S. record of 189 EC 136 213 66 rfcitoninae, notes on fossil Edmunds, G.F., Jr. Edwards, R.L., W.L. Gabriel Dry weight of fresh & preserved spiders Eisses, K.T., M. Santos 56 Species distinction in abdominal pigmentation patterns between females of Drosophila melanogaster & D. simulans from a Spanish population Entomobryidae 51 Ephemeroptera 2 1 , 27, 1 1 0, 1 1 7, 1 22, 129,213,215,257,261,266,268, 344, 345, 354, 357 Evaniodes spathiiformis, redescrip. of 269 Telix, M., G. Mejdalani 307 A n.sp. of Neotropical genus Bythonia, & female of B. consensa Fischer, C. 274 Foote, B.A. 256 Formicidae 81,88,136 Froeschner, R.C. 343 Two corrections: Aradidae, Tingidae VJabriel, W.L. 66 Grastrisus, a new Mexican sp. of 225 Gelastocoridae 33 Gerridae 191,233 Glossosomatidae 103 Cough, F, B.L. Haase 129 Diel emergence patterns of Tricory- thodes stygiatus on Little Lehigh Creek, Allentown, PA Haz lase, B.L. 129 Hamilton, IV, R., R.L. Petersen, R.M. 36 Duffield Unusual occurrence of caddisflies in a Penn. population of pitcher plants Hare, L. 285 Harmonia axyridis, impact of invasion 1 43 Harris, M.A. 315 Harris, S.C. 99 Hawks, D. 369 Helicoverpa armigera, gynandromorph 288 of Hemiptera 339, 366 Henson, R.N. 240 Heptageniidae 213 Heraty, J., D. Hawks 369 Hexamethyldisilazane, a chemical alternative for drying insects Heteroptera 33, 95, 1 9 1 , 253, 274, 343 Hexamethyldisilazane, a chemical 369 alternative for drying insects Hoekstra, J.D., R.L. Smith 366 Distrib. & habitat of Curicla pro- noiata in southeastern Ariz. Homoptera 297, 307 Houghton, D.C., K.W. Stewart 103 Seasonal flight periodicities of six microcaddisflies in Brazos River, Tex., with notes Houp, K.H. 99 Houp, R.E., K.H. Houp, S.C. Harris 99 Two n.sp. of microcaddisflies from Kentucky Hribar, L, J. 282 Biol. & morph. notes on Dasyhelea pseudoincisurata 378 ENTOMOLOGICAL NEWS Hubbard, T. 268 Lee, J. 215 Huisman, J. 37 Lepidoptera 288, 325, 329 Hydroptilidae 99, 103,256 Leptoceridae 37 Hymenoptera 1,7,81,88, 136, 198, Leptohyphidae 129 217,247,252,269,318,329 Lester, G. 213 Hynesionella, two n.sp. from South 191 Libellulidae 301 Africa Achneumonidae 329 Isotoma louisiana, reassignment of 195 Ixodidae 172 J amieson, W., V.H. Resh 329 Japygoidea 177 Josephrajkumar, B. Subrahmanyam, 288 V.V. Ramamurthy Gynandromorph of Helicoverpa armigera iVar 172 256 iresh, W.S. Keiper, J.B., D.A. Casamatta, B.A. Foote Incorporation of algae into cases of Ochrotrichia woicickyi Kennedy, J.H. 257 Kirchner, R.F. 293 Kondratieff, B.C., R. F. Kirchner 293 A n.sp. of Sweltsa from eastern No. Amer. Kritsky, G., A.J. Savage, S. Reidel, 165 J. Smith Survey of summer tiger beetles on Ohio River beaches in Ohio & Indiana Kurczewski, F.E. Comparison of two sand nesting 247 wasps from two pine barrens in upstate NY. Dispersal & range expansion of 1 introduced sand wasp, Oxybelus bipunctatus in northeastern No. Amer. Identity of Tachysphex acutus, 252 an unsolved mystery Kurczewski, F.E., D.L. Wochadlo 7 Relationship of cell depth & soil moisture in Oxybelus bipunctatus J-^abidognatha 66 Lambdin, P. 297 Cerococcus michaeli, a n.sp. of false pit scale from New Zealand Lingafelter, S.W. 75 Observations of interactive behavior in Parandra glabra Lugo-Ortiz, C.R. 117 Lugo-Ortiz, W.P. McCafferty A new No. Amer. genus of Baetidae 345 & key to Baetis complex genera First report & n.sp. of genus 1 22 Cloeodus from Australia N.sp. of Cloeon & Demoulinia from Madagascar M, 357 47 La, Y-T., K.A. Christiansen A n.sp. of Tomocerus from China Macrothemis inacuta, larva of 301 Mailing dates, Volume 109 375 Manley, D.G., T.P. Carithers 198 New host record for Sphaeropthalma pensylvanica Marquez, J. 225 Maxwell, J.A. 189 McCabe, T.L. 325 McCafferty, W.P. 1 1 0, 1 22, 345, 357 McCafferty, W.P. 266 Adds. & corrections to Ephemeroptera of No. Amer., & index to nomenclature McCafferty, W.P., T. Hubbard 268 Adds, to Iowa mayflies McCafferty, W.P., C.R. Lugo-Qrtiz 1 1 7 Adult of Moribae tis macaferti McCafferty, W.P., E.L. Silldorff 26 1 Reared assoc. & equivalency of Baetis adonis & B, caelestis McCafferty, W.P., R.D. Waltz 354 A n.sp. of small minnow mayfly genus Plauditus from So. Carolina McCafferty, W.P., T.-Q. Wang 344 New name change for a generic homonym in Teloganodidae McCorquodale, D.B. 15 Adventive lady beetles in eastern Nova Scotia McPherson, J.E. 95, 233 Megaloptera 285 Vol. 109, No. 5, November & December, 1998 379 Mejdalani, G. 307 Membership, Amer. Entomol. Soc. 219 Mesovelia cryptophila, lab. rearing of 95 Miller, S.S. 143 Moore, J.P. 363 Aedes albouictus occurrence throughout Tenn., with biol. notes Moribaetis macaferti, adult of 117 Muegge, M.A., C.E. Carlton 1 77 New records of Japygoidea from Louisiana, with behavior notes Mutillidae 198 N alepa, C.A., K.R. Ahlstrom, B.A. 277 Nault, J.L.Williams Mass appearance of lady beetles on No. Carolina beaches Niault, B.A. 277 Navarrete-Heredia, J.L., J. Marquez 225 A new Mexican species of Gastrisus Neogerris hesione, voltinism in, in 233 so. Illinois Neoperla coosa, a new stonefly sp. 1 53 from Alabama Nepidae Nerthra praecipua, redescrip. of, from Chile Noctuidae Novelo-Gutierrez, R., A. Ramirez Larva of Macrothemis inacuta O 366 33 288 301 256 'chrotrichia woicickyi, incorpo- ration of algae into cases of Ode, P. 215 Odonata 201,301 Odonata of so. central Nearctic 201 region, incl. northeastern Mexico Ownership statement 375 Oxybelus bipunctatus, Dispersal & range expansion of in 1 northeastern No. Amer. Relationship of cell depth & soil 7 moisture in Jointer, R.L.E. 172 Parandra glabra, Obs. on interactive 75 behavior Paruroctonus, a n.sp. of, from Big 240 BendNat'l. PL, TX Pentatomidae 274 Penteado-Dias, A.M. 269 Penteado-Dias, A.M. 217 First record of Aleiodes depanochora from Brazil Perlesta golconda, a new stonefly 315 sp. from Illinois Perlidae 153,315 Petersen, R.L. 36 Philips, T.K..D.C. Smith 159 Biol. & hosts of Procecidochares atra. evidence for cryptic species Phryganeidae 36 Plauditus, n.sp. of, from So. 354 Carolina Plecoptera 1 53, 1 83, 293, 3 1 5 Polhemus, J.T. 191 Two n.sp. Hynesionella from So. Africa Polhemus, J.T, T Cekalovic K. 33 Redescrip. of Nerthra praecipua from Chile Procecidochares atra, biol. & hosts 159 of: evidence for cryptic species Pseudocentropfeloides, a n.sp. of, 110 with revisions to other baetid sp., from Texas Publisher's statement 375 Purrington, F.F., J.A. Maxwell 1 89 First U.S. record of Dyschirius sextoni Pyralidae 329 Lamamurthy, V.V. 288 Ramirez, A. 301 Raptoheptagenia cruentata, new dis- 2 1 3 tribution of Reidel, S. 165 Resh, V.H. 329 Rider, D.A., C. Fischer 274 Zorcadium, an objective junior synonym of Pseudobebaeus Robbins, R.G., W.B. Karesh, R.L.E. 1 72 Painter, S. Rosenberg Ticks of genus Amblyomma from white-lipped peccaries in northeastern Bolivia, with comments Rosenberg, S. 172 380 ENTOMOLOGICAL NEWS Roy, I., L. Hare 285 Eastward range extension, in Canada, of alderfly Sialis velata, & potential of genus as contaminant monitor Sabrosky, C.W. 60 Santos, M. 56 Savage, A. J. 165 Scorpiones 240 Sialis velata, eastward range exten- 285 sion, in Canada, & potential of genus as contaminant monitor Silldorff, E.L. 261 Sissom, W.D., R.N. Henson 240 A n.sp. of Paruroctonus from Big BendNafl. Pk.,TX Smith, A.D.,B.P. Stark 153 Neoperla coosa, a new stonefly sp. from Alabama Smith, D.C. 159 Smith, J. 165 Smith, R.L. 366 Smithistruma memorialis, a n.sp. of 81 ant from Kentucky Cumberland Plateau Society meeting of 152,224,296 Sphaeropthalma pensylvanica, new 1 98 host record for Sphecidae 1,7,247,252 Staphlinidae 225 Stark, B.P. 153,315 Stewart, K.W. 103,211 Subrahmanyam, B. 288 Sweltsa, a n.sp. of, from eastern 293 No. Amer. Symphitoneuria, a n.sp. of, from 37 Sabah, Malaysia J. achyophex acutus, identity of, 252 an unsolved mystery Tanychela pilosa, biol. of, parasi- 329 toid of aquatic moth Petrophila confusalis Taylor, S.J., J.E. McPherson Lab. rearing of Mesovelia 95 cryptophila Voltinism in Neogerris hesione 233 in so. Illinois Teloganodidae 344 Tephritidae 159 Tingidae 343 Tomocerus, a n.sp. of, from Chile 47 Tomocerus spinulus, a n.sp. of 51 Chinese springtail Tormos, J. 318 Trichoptera 36, 37, 99, 103, 256 Tricorythodes stygiatus, diel emer- 1 29 gence patterns of, in Little Lehigh Creek, Allentown, PA Trogoderma granarium, recent inter- 20 ception of at port of Baltimore raejovidae 240 Wa faltz,R.D. 354 Waltz, R.D. 195 Reassignment of Isotoma louisiana Waltz, R.D., D.E. Baumgardner, J.H. 257 Kennedy Character variability & a new synonym of Acerpenna pygmaea Waltz, R.D., G.F. Edmunds, Jr., G. 213 Lester New distributions for Raptoheptagenia cruentata & Ametropus neavei Waltz, R.D., P. Ode, J. Lee 215 Cloeodes excogitalus in no. Calif. Wang, T.-Q. 344 Wiersema, N.A. Camelobaetidius variabilis, a n.sp. 7 from Tex., Okla., & Mexico Newly-reported & little known 27 mayflies of Texas Wiersema, N.A., W.P. McCafferty 1 1 0 A n.sp. of Pseudocentroptiloides, with revisions to other baetid sp. from TX Williams, J.L. 277 Wochadlo, D.L. 7 , M.L., J.A. Barron 20 Recent interception of live khapra beetle, Trogoderma granarium, at port of Baltimore Zorcadium, an objective junior 274 synonym of Pseudobebaeus When submitting papers, all authors are requested to (1) provide the names of two qualified individuals who have critically reviewed the manuscript before it is submitted and (2) suggest the names and addresses of two qualified authorities in the subject field to whom the manuscript may be referred by the editor for final review. All papers are submitted to recognized authorities for final review before acceptance. Titles should be carefully composed to reflect the true contents of the article, and be kept as brief as possible. Classification as to order and family should be included in the title, except where not pertinent. 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