s 2 4 wilted HE sate. et 3 4 * as topeaes eae : Se eeres Perr ee’ Cilin Wiepeue a Ss Op GS« LACHEN Lacien wl CuPEKARCS. Zen AL REPORT AND Teme NSACTIONS 7 £2 Sor mun. +/ 5? \ y SOCIETY OF FIELD NATURALISTS. ESTABLISHED 1871. SESSION 1872-78. PRINTED FOR THE SOCIETY BY THOMAS SMITH & CO., 257 ARGYLE STREET, GLASGOW. MDCCCLXXIII. f , >is an Se ner, se ee ay | aes < oh eg he | Anes. - he}, rel, i ‘aoe Tur Guiascow Society or Fretp Natvratists was established in May 1871, for the advancement and cultivation of the study of the various branches of Natural History ; for furthering original biological research among its members; for the elucidation of the Natural History of Clydesdale, more especially of the obscurer branches of Botany and. Zoology ; and for the spreading of a love for the study of Nature among the community. The means adopted for the promotion of these objects are (1), Meetings for the Exhibition of Specimens and the Reading of Papers ; (2), Excursions to places of interest in the vicinity of Glasgow ; and (3), by the formation of Typical Collections of the Smaller forms of life. OFFICE=-BEARERS: FAL is Mian ei aca tes J. Stirton, M.D. AMG-UUPRMENDG, <2. vccesauscaeede ove J. ALLAN and G. Horne, APM Sse os, dsrshbvorien thas .<> IS Wy a WY LALLA : ; Vl Fz. Zo. Ve ely Z NSS Www wy” AN aerete: fe tpg yee ot Ent GLASGOW SOCIETY OF FIELD NATURALISTS. 125 two systems of lenses, the anterior, consisting of a plano-convex front lens and a concavo-convex lens cemented together; the posterior combination consists of a plano-concave and bi-convex, (fig. 4) or of a bi-concave lens with a bi-convex lens above and below (fig. 5). In higher powers these lenses become more numerous, consisting of a triple anterior and posterior and a double middle combination as shown in diagram No. 8. Mr. F. H. Wenham has proved that a pencil of rays exceeding an angle of 40° from a luminous point, cannot be secured with less than three superposed lenses of increasing focus and diameter, but by the use of such a triple combination rays beyond this angle can be transmitted with successive reflections in their course towards the posterior conjugate focus. The first object-glass made by Mr. Ross in 1831, consisted of three bi-convex lenses of crown glass, each of which was achromatized by its own plano-concave lens of flint glass (fig. 6). It was in one of these glasses that Mr. Ross made the anterior combination adjustable in order to correct the errors of spherical aberration consequent upon using covered or uncovered objects. In 1837, Mr. Lister gave Mr. Ross a diagram for a 4-in., having a triple front lens to facilitate the passages of the extreme rays. In 1850, the same gentleman communicated to Mr. Wenham his invention of the triple back combination (fig. 8), which was a considerable improvement on the double one. Mr. Wenham, in the course of his trials, invented the single front of crown glass, the front part being cylindrical and the back convex. The height of the cylindrical part is one of the most essential parts of corrections, as for instance, in a 7; a difference of thickness of only -002 inch determines the quality between a good and an indifferent glass. This single front devised by Mr. Wenham has, after much hesitation on the part of other makers, been adopted by them all. Mr. Wenham constructed an excellent object-glass of five lenses only, and since then he has devised object-glasses of only four lenses as shown in the diagram, fig. 9, thus dispensing with six surfaces as formerly used. It is obvious that these objectives, if properly corrected, must give an increase of brilliancy and definition. Another advantage is, that high powers constructed on this plan can be used both as dry and as wet or immersion lenses, by simply adjusting the screw collar. The system of immersion lenses is due to Professor Amici, who discovered that by placing a small drop of water on the front lens 126 TRANSACTIONS OF THE which will unite with the covering glays, an increase of light and definition is obtained, as the most divergent rays are by. their passage through water, instead of through air, so refracted that they enter the object-glass. The object-glasses last described have been patented by Mr. Wenham, and they are made by the celebrated firm of Ross & Co. in London, at prices considerably below those of their great rival object-glassmakers, Powell & Lealand, whose } and ;,-in. immersion lenses have for a considerable time been regarded as the most perfect glasses made. The latter firm has just brought out a + and a 4 constructed on a new formula, which they say are superior to their former glasses of the same focal length. These new glasses cost, with immersion arrangements, eleven guineas a-plece. The qualities a first-rate object-glass should possess are, 1. Defining power, which depends upon the completeness of its corrections. 2. Penetrating power, or focal depth, which depends upon the degree of distinctness with which part of the objects that are a little out of focus may be seen. 3. Lesolving power, by which minute surface markings are seen and clearly separated from each other. 4, Flatness of field, which depends on the distinctness with which both the central and peripheral portions of an object are seen. An object-glass combining all the preceding attributes in the highest degree in which they are compatible with each other may be pronounced perfect. The optical qualities of an object-glass are examined by means of tests or test-objects, such as sections of spines of echinus, of deal, proboscis of fly, pygideum of flea, muscular fibres, finely marked valves of diatoms, scales of insects, especially the Podura scales, and Nobert’s test-plate having as many as 200,000 parallel lines to the inch ruled on a piece of glass with a diamond. I cannot refrain from mentioning here the marvellous Micro-engray- ings of Mr. W. Webb, whose finest engraving of the Lord’s Prayer is on a scale equal to that of writing the whole Bible fifty times in the space of only one square inch.* * These engravings, Nobert’s test-lines, Modller’s typenplatten and test- objects, are supplied by Mr. Edmund Wheeler, 48 Tollington Road, Holloway, London, N. EE SVASAAA RQ W\ J GLASGOW SOCIETY OF FIELD NATURALISTS. Tae The magnified image produced by the rays of light after having passed the object-glass and crossed in the focus of the back combination, are viewed by a simple microscope at the upper end of the body called the Hye-piece. The Huyghenian eye-piece (fig. 10), commonly used, consists of two plano-convex lenses with their convex sides directed towards the object-glass. The lower larger one is called the field-glass, the upper one the eye-glass. These eye-pieces give a field of from 4 to 6 inches diameter. The Huyghenian eye-piece is not achromatic, being under corrected for colour, and to secure achromatism of the microscope all good object-glasses are so constructed that they are over corrected for colour. The Kellner or orthoscopic eye-piece (fig. 11) has a double convex lens placed in the focus of the achromatic eye-glass without the interposition of a diaphragm. It does not give quite as good definition as the ordinary eye-piece, but it yields a large, flat, well- lit-uwp field of from 12 to 14 inches diameter. I can confidently recommend the Kellner eye-piece No. C, as made by Ross, but these Kellner eye-pieces should not be bought in pairs. To every first-class microscope there belong a number of eye- pieces of various powers, which English makers commonly designate by A, B, C, D, E, and F; A being the shallowest and least power- ful, F the deepest and most powerful, magnifying diametrically about eight times as much as A. Although a great increase of magnification is obtained by the use of high eye-pieces, these magnifications are not of great value, as the brilliancy with which an object appears illuminated is reduced in inverse ratio to the superficial magnifying power ; thus by the use of eye-piece F, the object appears only 1-64th as brightly Jit up as under A. In addition to the loss of light, the errors of the object-glass are magnified without gaining any compensation in focal depth. More- over, high eye-pieces, owing to their short foci, are very trying to the eyes. In fact the use of high eye-pieces recommends itself, only when without the trouble of changing the object-glass a large magnification has to be obtained, good definition being only a matter of secondary importance. The magnification of a micro- scope may also be increased without impairing the definition per- ceptibly, especially when shallow eye-pieces are used, by increasing the distance between the object-glass and the eye-piece by means of a draw-tube, which is generally graduated. 128 TRANSACTIONS OF THE A microscope having only one body, and which can only be used with one eye at a time, is called monocular ; a microscope having two tubes and eye-pieces, allowing an object to be viewed simultaneously with both eyes, is called binocular. The advan- tages of a binocular microscope over a monocular one are the following :— 1. It is pleasanter and less trying to the eyes, permitting con- sequently a far more prolonged use. 2. Much greater focal depth is gained. 3. A stereoscopic effect is obtained, giving the observer a more correct idea of the shape of the object under observation; and 4. By its use the mind is relieved of the constructive effort which it has to make in order to realize the shape of the object or its details, when viewed only with one eye. Altogether, I cannot sufficiently advocate the use of the binocular microscope; every one who values his eyesight, and has to make prolonged microscopical observations, should use a binocular. An observer using a monocular microscope can form no better idea of the object he views than a man using one eye alone can judge of distance and form in a strange place. J am aware that many scientific men, especially some old microscopists, still deprecate the use of the binocular, but this arises either simply from prejudice, or because they have only seen instruments with faulty binocular arrangements, or because their eyes have different foci. The best binocular arrangement is undoubtedly that invented by Ur. F. H. Wenham in 1860, and consists of a very small prism of peculiar form (Fig. 12), which is placed in a small frame in the nose-piece or the lower end of the body of the microscope. The lower surface of this prism intercepts exactly half of the cone of light coming from the object-glass, and reflects them up the slanting tube, whilst the other half of the rays passes through the reflected bundle of rays straight in the eye-piece above the object- glass. By the withdrawal of the prism the binocular microscope is instantly converted into a monocular one. The effect of this splendid arrangement is truly stereoscopic ; unfortunately it can ouly be used with low powers, namely, up to the one half-inch object-glass inclusive, and only then when the latter has not more than 40° aperture. For high powers one of these small Wenham prisms would require to be fitted immediately behind the posterior combination of lenses of GLASGOW SOCIETY OF FIELD NATURALISTS, 129 each object-glass, which is both expensive and not very practicable. Various methods for obtaining stereoscopic binocular vision under high and the highest powers have been devised, and the best and the one most in use seems to be the one invented by Mr. Stephen- son. In his apparatus the rays on emerging from the object-glass pass through two small prisms, but owing to their considerable length a great deal of light is abstracted. These binocular arrange- ments for bringing into each eye only one-half of the bundle or cone of rays which have passed through the objective, act stereo- scopically. Other methods of enabling the observer to use both eyes have been devised, as for instance Powell and Lealand’s patent, but as they reflect identical wmages into the two eye-pieces their effect is non- or pseudo-stereoscopic. The stand of the compound microscope consists of the support for the body, the focal adjustment, the stage to carry the object, a mirror for illuminating the latter, and which is attached to the so-called tail-piece, and a diaphragm beneath the stage to regulate the quantity of light reflected from the mirror. The most com- plete microscope stands have an additional sub-stage for holding the various pieces of illumination apparatus, which in second or third-class instruments have to be placed into a short tube-fitting attached to the stage. The stand must be heavy, in order to be quite steady and free from tremor. There is a great variety of models of stands, but I shall only mention a few of the principal ones. There is first a draw-tube stand, which we find in all old microscopes, and which is still retained by most German and French makers, notably in the excellent microscopes of Hartnacks and Nachet, which I cannot sufficiently recommend on account of their portability, cheapness, excellence of workmanship, and high quality of object-glasses. Then there is the so-called Ross model, in which the body is supported by a rectangular arm ; and there is the Jackson model, the steadiest of all, in which the body is supported along the greatest part of its length. Even the great house of Ross & Co, has, at the suggestion of Mr. Wenham, adopted the Jackson model in 1874, and their new stand I consider to be the handsomest and best of all. The coarse adjustment consists in simple microscopes of a draw- tube, and in the better English models of a rack and pinion move- ment by means of large milled heads. The fine adjustment which in Ross’ new model is placed behind the body to protect it from S 130 TRANSACTIONS OF THE injury, consists of a fine micrometer screw, having in good micro- scopes from 100 to 200 turns to the inch. This screw acts by means of a lever upon the so-called nogse-piece in the lower end of the body into which the object-glass screws, and which is pressed downwards by means of a spring. I must not forget to state that the Royal Microscopical Society in London has introduced a stand- ard gauge of screw, with which the nose-pieces of all good English microscopes and all good English object-glasses are now-a-days provided. By means of one of the best fine adjustments we can easily focus through a distance of only the one five-thousandth part of an inch, and it serves at the same time to measure roughly the height or depth of small objects. The stage may either be plain or mechanical, and a good plain stage is infinitely preferable to a bad mechanical one. The stage should be large, have a large opening in the centre, and ought to be as thin as possible, to allow of the most oblique illumination. There are various contrivances in use to assist In moving the slide, but the ordinary mechanical stage consists of rectangular rack and pinion and screw movements. In addition to these rectangular movements there is a circular movement which is seldom mechani- cal. The latest perfection of the stage is the concentric rotating movement, which allows of an object being kept in the field of view when the stage is rotated. In order to obtain this concentric rotating movement, the rectangular movements are placed on the rotating movement, and not the rotating stage on the rectangular movement, as in the ordinary mechanical stage. To some simple instrument without rectangular movements a concentric stage is sometimes added, which is generally made of glass. In the larger instruments the concentric rotating stage is graduated into 360°. The diaphragm is placed under the stage or in the sub-stage. It consists in its simplest form of a disc of metal having various sized apertures for admitting more or less light on the object. The graduating diaphragm is a superior contrivance for gradually reducing or increasing the aperture of the diaphragm, which is of great importance for obtaining the best effects. The sub-stage is only added to first-class and to some of the second-class microscopes. It has generally a rotating movement by means of a wheel and pinion and two centring screws. A rack and pinion raises or lowers the sub-stage, which is also generally graduated into 360°. Ordinary microscopes have, as mentioned GLASGOW SOCIETY OF FIELD NATURALISTS. 131 already, a tube-fitting attached to the stage into which the sub-stage illumination apparatus is fixed by means of a bayonet catch, but the length of this tube prevents oblique illumination by means of the mirror or prisms. On the tail-piece of the microscope a large murror held by a jointed, often doubly jointed, arm can be made to slide up and down. One side of the mirror is generally plane and the other concave. Having described the essential parts of the microscope itself, I will now enumerate and explain those accessories which the microscopist will find useful and often indispensable. 1. The stage or mineral forceps for holding unmounted objects, such as insects, parts of plants, minerals, ce. 2. The stage plate, which is made of glass and has a glass ledge, serves for unmounted objects, especially fluids, which might injure the stage if they came in contact with it. 3. The Polyp or Zoophyte trough of ground glass, for viewing Polyps, &c., in water. 4, The live-box, of which it is well to have various sizes. It consists of a circular box fixed on a plate ail of brass and having a glass bottom and a thin glass cap to slide over it. A convenient form for condenser illumination is the live-box with flush glass bottom. ‘These live-boxes are used for the examination of live objects such as insects, but especially for Infusorie. 5. The compresser is an instrument consisting of two plane parallel glass surfaces, which, by means of levers, screws, or wedges, may be more or less approached to each other. These compressers serve to apply a gradual pressure to objects whose structure can be better, or can only then be, seen when they are flattened out. Such pressure may be applied to crush small shells, to flatten a cuticle, to hold larger animalcules in the same place so that they are unable to move out of the field of vision, &c. Strict parallel- ism of the surfaces is most essential, especially for the examination of fluids, which otherwise would be at once squeezed out in one direction instead of being spread out. 6. The frog and fish plate for examining the circulation of the blood in the web of a frog’s foot, or in the tail or fins of a fish, the animal being wrapped in a little piece of wetted linen and tied by means of threads to the plate which is then fixed on the stage. 7. The double, triple, or quadruple object-glass carrier, or Brooke's arm or double nose-piece, which screws into the nose-piece of the 132 TRANSACTIONS OF THE microscope body and can be swung round for the purpose of bring- ing rapidly another object-glass in position. The double nose-piece is handier than the triple or quadruple one, but only the bent form as devised by Powell and Lealand should be bought, as it is the safest, carrying the objectives which are not in use entirely off the stage. 8. The camera lucida, a small instrument fitting over the eye- piece and reflecting the image on a sheet of paper on the table when the microscope is placed horizontally, so that the outlines of an image can easily be traced and measured. The observer must look with the upper half of the pupil of one of his eyes on the prism or on the steel or glass disc, and with the lower half of the pupil he requires to follow the pencil point. The illumination must not be too bright, else the pencil point is hardly seen. The best form is Wollaston’s prism camera-lucida. To measure the magnification a micrometer, 7.e., a slip of glass, divided into hun- dredths, thousandths, or ten-thousandths of an inch, must be placed on the stage and focussed, and the magnified image of these divisions traced by means of the camera and measured. 9. The eye-piece micrometer for measuring objects in the micre- scope consists of a micrometer either permanently or temporarily fixed between the field and the eye-glass of the eye-piece, and after the value of its divisions has been ascertained by means of a stage micrometer, the sizes of objects under observation may easily be read off. Jackson’s micrometer is a scale on glass in a brass frame which can be inserted into the eye-piece, and which is further adjustable by means of a very finely divided screw. Aamsden’s screw micrometer is the most perfect apparatus for measuring the size of objects in the microscope, and consists of an eye-piece containing two parallel thin wires which can be separated or approached by means of a screw. Up to the 1-75,000th of an inch can be measured by means of this instrument. The simplest way of measuring roughly the size of the magnified image is to look down the microscope with one eye and with the other eye to look on a scale held close to the body of the instru- ment at a distance of 10 inches from the eye. In this position the observer sees both object and scale simultaneously, and can read off the size with tolerable accuracy. 10. The eye-piece goniometer serves for measuring the angles of crystals, &e., by means of a scale in the eye-piece, which can be GLASGOW SOCIETY OF FIELD NATURALISTS. eve made to revolve with it if a milled head is turned. The size of the angle is read off on a brass plate which is divided into 360°. 11. The erector or erecting glass, for showing the inverted image in the same position as the object, is useful when a dissection is to be made under the microscope. It consists of a tube carrying three lenses, which is fixed into the lower end of the draw-tube, and by its means the image can be more or less magnified. 12. The centring glass, fitting over the eye-piece, serves for testing the centricity of the illuminating apparatus and of the sub-stage. It has a minute aperture, and by looking through it both the diaphragms of the object-glass and of the condenser are seen, which must appear concentric if the sub-stage is properly centred. 13. The object-finder, for finding objects on slides previously seen, and the position of which has been registered. I will only explain Mr. Maltwood’s finder, which consists of a small plate of glass on which a field of one square inch is photographed. This field is divided by horizontal and vertical lines into 2500 small squares, each of which bears a different number. Jf the position of an object under the microscope is to be registered, the Maltwood finder is placed in exactly the same position as the slide, which is resting against the ledge of the stage, and against a pin fixed on it. The number of the corresponding field is then read off and either marked on the label of the slide-or in the catalogue. By reversing this proceeding the registered object is easily found again. I have now arrived at the last chapter of my lecture, in which I shall speak of mucroscopic ilunuination and wdluminating apparatus. Illumination in microscopy is the principal thing, and successful observation, especially under high powers, is mainly dependent upon proper illumination, for by its means inferior object-glasses may be made to show more than the best objectives will show if the object under examination is not properly illumined. The first thing requisite is good light, and the light best suited for magnifi- cation, up to 2000 diameters, is daylight reflected by a white cloud, against which the sun shines. Such light is, at the same time, least detrimental to the eye-sight. For the highest powers, and for the resolution of the most difficult tests, sunlight may be employed, but, owing to its great brilliancy, it should be modified by the interpolation of a light modifier of roughly ground white glass, or it 134 TRANSACTIONS OF THE should not be used from the mirror, but should be reflected from a piece of plate glass, under which fine black silk velvet has been laid. As the ordinary white light is often dispersed into prismatic colours by the nature and form of the objects themselves, resulting in impaired definition and resolution, this difficulty may be overcome by the use of monochromatic light or light of one colour. Such light may be obtained from the spectrum, or by passing the white light through coloured glass or through a flat glass cell containing an ammoniacal solution of sulphate of copper, the blue and violet rays being the most suitable owing to the greater length of their waves. Such coloured glasses or cells containing coloured solutions are called light modifiers. As daylight, and especially sunlight, are scarce commodities in this country, at least in our large cities, and as the amateur microscopist has generally to pursue his observations in the evening after his day’s work is done, recourse must be had to artificial light, which indeed, especially for high powers, is the handiest, and suffices for all but the resolution of a few of the most difficult tests. Gaslight is not very suitable owing to the ever varying pressure in the main, in consequence of which the flame does not burn steadily enough. An ordinary paraffin or camphine lamp for one shilling will do very well as far as light is concerned, but I recommend to you How's Microscope Lamp and also the so-called Bocket Lamp. How’s lamp consists of a glass reservoir holding sufficient oil to last for four to five hours, and is adjustable on a brass rod fixed in a large circular foot. A ring of stout wire holds one of Hale’s porcelain shades, which has only one opening in front to allow of the passage of the rays; this shade keeps in the heat, protects the eyes from the glare, and reflects a white light. Every one using a microscope regularly, even if it be only a small student’s microscope, should provide himself with one of these lamps; he will find that the few shillings it costs are well invested. A handsomer lamp is Swift's, which packs in little space and has an additional reflector and bull’s-eye condenser with blue light modifier turning on the same arm. ‘The glass stopper in the reservoir for introducing a further supply of oil when necessary is, in my opinion, no great improve- ment, as the oil oozes out when the lamp gets hot. Middzan’s Microscope Lamp has a metallic chimney which is internally coated with plaster of Paris, and has a white cloud reflector, neutral tint-shade, &c. It is the most complete and best lamp made, but GLASGOW SOCIETY OF FIELD NATURALISTS. 135 costs about six pounds. A microscope lamp must have its burner adjustable in vertical directions and allow of its being revolved, or it does not deserve the name, although various patterns not having these properties are made and sold as microscope lamps. For bringing out markings and for obtaining very intense illumin- ation the narrow side of the wick should be used : indeed, various sides of the flame should always be tried, as by this means the best intensity of light can easily be ascertained. In the illumination of objects the rays ought to be parallel in nearly all cases. By paying attention to this, most structures of transparent objects can be made out by the mirror or rectangular prism alone. The angle of illumination should not be excessive, and should not exceed 90 degs. Objects are either opaque or transparent, and the latter may be viewed by transmitted or by reflected light. The simplest means of eluminating opaque objects is by means of the so-called bull’s-eye condenser, which is a plano-convex lens mounted on a separate stand, or fitting into the stage or the stand of the microscope ; the latter form is called a stage condenser, and isnot very useful. The convex side should always be turned towards the source of light in order to obtain parallel rays, else by the crossing and recrossing of an infinite number of rays all the shadows will be destroyed which are absolutely requisite to bring out the shape and nature of the surface of the object under examination. Greater brilliancy is obtained by passing the rays first through a bull’s-eye condenser on to a silvered side reflector of parabolic surface, often called a speculum, which in its turn reflects the rays on the object. Objects which are mounted on an opaque ground, about 2-inch or less diameter, may be illuminated by means of the Lreberkuhn (figure 13), which resembles the mouth-piece of a wind instrument, and is silvered on its hollow parabolic surface. It is slid over the object-glass, and the rays are reflected on it from the plane mirror. The Lieberkuhn’s parabolic surface reflects in its turn the light on the object, which is placed in its focus. A separate Lieberkuhn is required for each object- glass, and the highest power under which an opaque object can be illuminated with a Lieberkuhn, a parabolic side reflector, or the bull’s-eye condenser, is the }-inch, or a very narrow-angled finch glass, provided they have a long working distance. Many microscopists condemn the Lieberkuhn as throwing light from all 136 TRANSACTIONS OF THE sides on the object, thus allowing of no shadows; but this holds only good so long as the mirror throws up rays parallel to the axis of the microscope. Altogether, I consider the Lieberkuhn the easiest, and an excellent piece of apparatus for the illumination of opaque objects. Lister's dark wells are little blackened cups supported at the end of wires, and if one is placed by means of an adapter in the sub-stage, it intercepts the central rays from the mirror, and the transparent object having now a dark ground, may be viewed by means of the Lieberkuhn. The apparatus for viewing opaque objects, especially covered ones under high powers, are not very satisfactory. 2. & J. Beck's patent illuminator is one of the best, and consists in an adapter which has to be screwed between the nose-piece and the object- glass. It has a circular aperture, and a disc of very thin glass placed under an angle of 45 degs. By its means the object-glass is made its own illuminator, the light being reflected through the aperture on the glass disc and from thence on the uncovered object, which in its turn reflects the light back again through the object-glass in the eye-piece. Transparent objects may be viewed either by transimitted or by reflected light; the latter is called dark ground illumination. The simplest way of viewing transparent objects is by means of the plane mirror, on which parallel rays are thrown by the bull’s-eye condenser. For thick objects direct rays are necessary, but for thin and lined objects oblique rays are essential ; they are easily obtained by throwing the mirror out of the axis of the microscope. When the mirror is so much out of the centre that the parallel rays reflected from it pass so obliquely over the object that they do not enter the object-glass, we gain dark ground wdlumination, the object then appearing self-luminous and brilliantly lit up on a black ground. As the mirror gives a double reflection, namely, one from the outer surface, and one from the inner or silvered side, and as these two sets of rays are not parallel, those having passed through the glass being refracted, a rectangular prism is often substituted for the mirror. Such a prism reflects the light totally, and gives therefore more, and only parallel, rays; it is generally fitted to the tail-piece of the microscope, but it may also be mounted on a stand similar to that of the bull’s-eye condenser. Two such prisms enable the microscopist, who has good object- glasses but only a small and simple stand, to resolve the most GLASGOW SOCIETY OF FIELD NATURALISTS. 137 difficult lined tests, provided the stage be very thin to allow of very oblique illumination. This arrangement is also cheaper than an expensive condenser, but two lamps are required for it. Modifications of the rectangular prism are the Nachet and the Amici Prism (Fig. 14), which serve both to reflect and to condense the light. I can highly recommend the Amici prism as an excel- lent piece of apparatus for the resolution of lined objects, as it gives rays of great obliquity if thrown out of the axis, or intense direct light if placed in the axis, of the microscope. This prism, although small, gives sufficient light for magnifications of thousands of diameters, and is very easily used, but it is not achromatic. The best appliances for obtaining various quantities and qualities of light for the illumination of transparent objects are the achro- matic condensers. Any achromatic object-glass of medium power placed under the stage with its anterior lens nearest the object, will act as a good condenser. Opticians supply special achromatic combinations for this purpose, having close to the lower lens one or two diaphragms with various apertures and stops to regulate both the quantity and quality of light. The stops are generally six in number, namely, three central dark ground spots of various sizes for excluding the central rays, one lateral stop, one having two slots forming a right angle, and one stop having two slots forming an obtuse angle. In Ross’ condenser the pencil of light may be reduced from 110° to 20° by the circular holes in the upper diaphragm. It is considered the most useful condenser, and suffices for the resolution of the most difficult tests. Powell and Lealand’s condenser is similar, but has 170° angular aperture, and consequently a shorter working distance, but it is the best for the highest powers. Guillet’s condenser has the diaphragm in the shape of a flat ring which stands nearly perpendicular, and whose various apertures can be brought successively under the field lens. If parallel markings are to be resolved, it is evident that the unilateral stop has to be used, and that the condenser has to be rotated until the light falls perpendicularly against the markings or ribs; for cross markings the right or obtuse angle stops have to be used, for obtaining dark ground illumination under low powers or for great obliquity of light under high powers the central dark ground spots have to be employed. Mr. Reade’s hemispherical condenser consists of two plano-convex lenses with the plane surfaces directed upwards, and yields very oblique and i 138 TRANSACTIONS OF THE even monochromatic light. The Kellner eye-piece C, if placed in the sub-stage and provided with a number of stops fitting over the eye-lens instead of the cap, makes a very good condenser and yields abundant light up to 10,000 diameter magnification. The Webster and Swift condensers are each of them a multum in parvo, wniting in an efficient and not expensive way all the various pieces of sub-stage illumination apparatus, and will be found quite satisfactory in nearly all cases. I have obtained excellent results with the paraboloid for light ground illumination under the highest powers, as it gives plenty of oblique and white light. In fact I have, by its aid, resolved certain markings which some first-class achromatic condensers refused to bring out. The diffusion condenser, invented by Mr. Wenham, gives splendid effects on transparent objects viewed under the stereoscopic binocular microscope. It consists of an achromatic combination of 170°, having a white cloud cap, 2.¢, a cap of two thin glass discs between which some powdered glass is placed, which breaks up the pencils of light in all directions; it yields a soft white light, and by its means the two fields of the binocular are easily illuminated. This is sometimes a difficulty under medium powers, which may be overcome by placing a piece of roughened glass under the object. The object should be well lit up, but not too much so, else the binocular effect will be destroyed. Beginners generally think that the best effects are obtained by flooding the field with light, which is a very great mistake. The next class of condensers are those for dark ground wlumin- ation of transparent objects, the simplest is the spot-lens. This is a large plano-convex or bi-convex lens, the upper and lower surfaces of which with the exception of a narrow border are ground flat and blackened, so that if the light from the mirror is thrown on it, the marginal rays alone pass through it, and these being much refracted pass over the object without entering the object-glass ; the result is brilliant illumination of the objects on a black ground. The spot-lens is suitable for object-glasses up to half-inch focus. A more perfect instrument for the same purpose is the Paraboloid (Fig. 15) of Mr. Wenham. A parabola is a curve produced by the section of a cone and of a plane under certain conditions, and it has the property of reflecting all rays, which fall on it parallel to its axis, to one point in the same, called the focus. By rotating the parabola round its axis, we get a paraboloid which has the ZLN | | | ] | | | ! YNAS | \py N . * a ~ 5 . - ’ a 2 / ' a . 3 ~— ‘ < ’ : + a . ' ‘ 4 ' ' eG . - 7 ml . , ‘ ret / " w - i ey ¢ ; f t 2 / ! J P = ¥ ra / / ry 4 - . ‘ pail ’ . 1 f =~ 34 ~ A t = : ‘ ? . fi i ’ < . “ # - p < , > U! . . ¥ > r 4 ‘ . r ae s, GLASGOW SOCIETY OF FIELD NATURALISTS. 139 identical property with the parabola. Mr. Wenham’s paraboloid has its apex ground off down to }-inch below the focus, and a dark- ground stop in the centre. If the light is reflected from the plane mirror or rectangular prism on the paraboloid, an object placed in its focus will appear brilliantly lit up on dark ground. The para- boloid yields a blacker ground than the spot-lens, and the rays passing out of it in a more horizontal direction, it can be employed with the 1-5th object-glass. Wenham’s Reflex Illuminator (Fig. 16) is another most ingenious contrivance for obtaining oblique dark-ground illumination under the highest powers, provided the objects to be examined by its aid are suitable ones, attached to the slide and not to the cover, and mounted dry, that is, not in balsam or any other medium. This reflex illuminator is composed of a glass cylinder half-an-inch long and 4-10ths inch diameter, the lower convex surface of which is polished to a radius of 4-10ths inch. The top is flat. Starting from the bottom edge the cylinder is worked off to a polished face at an angle of 64°. Close beneath the cylinder is set a plain con- vex lens of 1}-inch focus. Parallel rays thrown up through the apparatus are reflected from the polished off plane of the cylinder on to the glass-slide, at an angle of total reflection; but if a suit- able object adheres to the surface of the slide, the light reaches it on an angle that admits of its passage. In order to ensure the continuous passage of the rays to the object, the top of the glass cylinder and the lower surface of the slide must be united by a film of water. The object appears then brilliantly lit up upon a dark ground, and when the apparatus is rotated splendid effects and an exquisite unfolding of structure is obtained. Nothing remains now but to speak of microscopical illumination with polarized light. I mentioned at the outset that light consists of the undulations of the luminiferous ether, and that these undu- lations take place in two planes at right angles to the ray. The object of the polarization is to bring these two sets of undulations into one plane, and this is effected by double reflection. An apparatus which polarizes light is called a Polariscope, and the phenomenon of polarization is one of the most gorgeous in nature, and serves at the same time the practical purpose or enabling the microscopist to form conclusions as to the nature of the object under observation by polarized light. The dfcro- polariscope consists of two prisms made of Iceland spar, or 140 TRANSACTIONS OF THE carbonate of lime, which crystallizes in rhombs and gives a double reflection. Mr. Nicol first succeeded in making single image prisms of Iceland spar, which reflect only the ordinary or the extraordinary ray at the time. A polariscope consists of two such prisms, one of which is placed under the stage below the object and is called the Polarizer, and the other is either placed behind the object-glass or above the eye-piece and is called the Analyzer ; both Polarizer and Analyzer are made to turn, If then an object such as doubly refractive crystals are placed on the stage, and either of the prisms is turned, they will exhibit the polarization of light in gorgeous colours. If objects are only simply refractive they require the interposition of a film of. selenite, which according to its thickness will exhibit two com- plementary colours such as red and green, blue and yellow, &c. By combining several selenites a large variety of colours and tints may be obtained during half a revolution. Selenite is the native crystallized hydrated sulphate of lime. The best arrangement of selenites is Darker’s, who places three selenite films of various thicknesses in a box in which they can be rotated both singly and in combination with each other. The variety of tints of a selenite may be greatly increased by interpolating a rotating film of nuca, and if one such mica film is combined with two selenites, the one blue and yellow and the other red and green, the entire series of tints which are produced by any number of selenites combined can be obtained. ‘The polariscope may be combined with the spot-lens or paraboloid for a dark-ground polariscope. If the analyzer consists of a double image prism or an original rhomb of Iceland spar, and if a brass plate with a small hole be placed on the stage, two magni- fied images of the latter will be seen which exhibit complementary colours, and if the hole be large enough the two images will partly overlap each other, and that portion where they overlap will look white, thus proving that the complementary colours produce white light. The objects especially suited for the polariscope are chemical crystals, hairs, fish scales, sections of horns and hoof, starch grains, vegetable and muscular fibres, the palates or lingual bands of molluscs, &c. Crystals are thus seen as they cannot be seen otherwise, and the chemically distinct constituents of vegetable and animal tissues become apparent in a way which no mere magnifying power could resolve. By the addition of a polariscope a great increase of power is thus gained. GLASGOW SOCIETY OF FIELD NATURALISTS. 141 In conclusion I will only mention that the spectroscope has also been combined with the microscope, and that such a combination is termed a Micro-spectroscope. 7tH DrcEeMBER, 1875. Mr. Alex. Noble in the chair. Mr. John M‘Glashan, 114 Candleriggs, and Mr. George Sturrock, were elected members of the Society. SPECIMENS EXHIBITED. By Mr. R. H. Paterson.—Specimens of various edible fungi from Cadder Wilderness. Among them were fine specimens of the “ Chantarelle” of the French (Cantharellus cibarius); two of the Milky Fungi (Lactarius piperatus and L. insulsus); the Hedge- hog Mushroom (Hydnum repandum); Clavaria flava, and C. ame- thystina,—the latter rare in Scotland. He also exhibited three of the British sponges from the Dornoch Frith, and a specimen of Lycopodium squamatum. This plant grows in the arid deserts of Central South America, and is possessed of remarkable hygro- metric properties. In the dry season it curls up its leaves and contracts its roots so as to form a ball, which lies on the surface of the sand and is blown about from place to place. As soon how- ever as it meets with wet ground, or on return of wet weather, it sends its roots down into the sand and unfolds its leaves, thus exchanging death for life. PAPER READ. By Mr. Thomas King.—On the “ Classification of the Mam- malia.” Beginning with the highest order, Bimana, in which we had man, he went over the lower orders, describing the distin- guishing characteristics of each, making some important remarks on the habits and distribution of the more important animals included under each order. Next to the Bimana we have the Quadrumana, which includes the monkeys of the Old and New Worlds. Some of the monkeys of the Old World had no tail, and none of them had a prehensile one; whereas all those of the New World had a long tail, generally prehensile. In the former we had the Orang-outang and Blue-faced Baboon, and in the latter such 142 TRANSACTIONS OF THE animals as the Spider-monkeys and the Howlers. In the Jnsectivora we had no very marked characters. It includes such animals as the mole, shrew, and hedgehog. To this group belongs the smallest of ali mammals, Sorex Htruscus, which is only two and a half inches in length including the tail. Passing on we come to the Chetroptera or bats. In this order the bones of the hands are greatly elongated, and have a membrane stretched between the separate fingers which is attached to the sides of the anima] and to the feet. It is this large membrane which gives to the animals their power of flight. In Britain there are several distinct species of bats, all of them being very small compared with those of other countries. The Rodentia, or gnawing animals, are characterized by the possession of two long incisor teeth in each jaw separated by a gap from the molars. These teeth are continually growing, and if from any cause one be broken the corresponding one in the other jaw elongates to a great extent. To this group belong such animals as the rat, hare, and beaver. The Carnivora are divided into three groups—the Digitigrada, or animals that walk on their toes, such as the lion and cat; the Plantigrada, animals that walk on the soles of their feet, as in the bears ; the Pinnigrada, in which the legs form paddles, like what we find in the seals of our shores, The Proboscidea are characterised by the absence of canine teeth; the incisor teeth are enormously developed and are termed tusks, as in the elephant and mammoth. The Ungulata include the Rumin- antia, such as the oxen, sheep, and deer; the Pachydermata, or thick-skinned animals; and the Solidungula, where the foot is terminated by a single toe, as in the horse. The next group, Cetacea, includes those animals which have a more or less fish-like body, as in the whales and porpoises. The order Marsupiala includes such animals as the kangaroo, and is characterised by the possession of a pouch in which the young are reared. The animals are chiefly inhabitants of Australia. In the last and lowest group, Monotremata, the jaws are destitute of teeth, and present the char- acters of the bills of birds. It includes the duck-billed animal and the echidna. The paper was illustrated with numerous diagrams and specimens. 21st Drecemper, 1875. Mr. James Allan, Vice-President, in the chair. GLASGOW SOCIETY OF FIELD NATURALISTS. 143 Mr. Robert Walker, 6 Nelson Terrace, Hillhead, was elected a member of the Society. SPECIMENS EXHIBITED. By Mr. R. H. Paterson.—A collection of lichens: Scyphophorus alicornis, S. bellidiflora, S. deformis, S. fimbriatus, S. coccifera, S. gracilis, Cenomyce pigridate. Also the fungus Polyporus gigan- teus. The last from the Cameroons, Africa. PAPER READ. Mr. J. Galbraith, M.A., read a paper on “ Volcanism,” or, as he said he would prefer to have it called, “‘ Vulcanology ”—the science which treats of voleanoes and earthquakes, and the causes which produce them. After reviewing the various theories of Sir Humphry Davy, Mallet, Hopkins, and others, he went on to pro- pound a theory which might, to some extent at least, harmonize the whole ofthem. He afterwards gave an account of the various manners in which changes of igneous origin are produced on the earth’s crust, of the best known volcanic eruptions which had occurred, and of the active volcanoes which are best known to science. lltH January, 1876. Mr. Richard M‘Kay, Vice-President, in the chair. PAPERS READ. Mr. James Allan read a paper on the “ Migration of Birds.” He began by noticing the wonder excited by the disappearance of birds, and the legends connected therewith, and the omens drawn from their flight by the ancients. Partial migration occurred in the case of the lapwing, which leaves the bare moorlands in winter and proceeds to the sheltered valleys. He laid before the meeting the following series of tables, in which the arrivals and departures of various birds were registered, extending over a series of years. He found that the corncrake seemed to be the most regular in its return, and the swallow the most irregular. But taking the average of any one year, it cannot be said that in such a season all birds appeared later or earlier. The chiff-chaff is 144 generally the first to arrive. TRANSACTIONS OF THE He considered that the return of birds was not connected so much with the state of weather of the country to which they come, as with the meteorological conditions of the country which they leave. He proposes to continue his registry of bird migrations over a wider area and embracing a longer period, and hopes to obtain more reliable results therefrom. In the following tables the double figures indicate the day and month, as 25/4, the twenty-fifth of April or 4th month. Lanpraliu (Gallinula Crea), 1864/1865 Bradford-on- Avon, . Uttoxeter, . Barnsleyee.ce sx Mansfield, bes Masham, York- shire, Oe cer ecenee Fochabers, ....... Kent, Middlesex, . Somerset, ........ INIOHUSM aes seneaees Derby, Cumberland, . ) 10/5 6/5 12/5 eee eee tonnes 1866 1867/1868 27/4| 26/4) 28/4| 26/4 6/5 | 5/5 | 9/5 | 5/5 2/5 |3/5 | 1/5 | 6/5 10/5] 11/5] 11/5]... oleae... 12a 180911870 1871)1872|1873|1874/1875 23/4 Bee i es 15/4 29/4| 30/4) 26/4 2/4 26/4| 2/5 Pata lies il sate . | 6/5 * és 15 2/5 27/4 r |. [2/5 19/5 | 6/5 | 2/5 27/4| 3/5 | 1/5 | 5/5 | 2/5 | 3/5 14/5 2 “6 me . 11/5 PAYED le ae ... | 12/5) 5/5 Waite TuHroat (Curruca cinerea). 1865 Mansfield, Masham, York, Ipswich, Barnsley, sas Bromley, 14/4 in 1846] ... Corwen, . ; Fochabers, Kent, Oe sewer eee Owe ee ree tweens ee a | 1866|1867 25/4] 25/4| 25/4) 25/4 Middlesex, Somerset, ........... Notts, Derbyshire, Westmoreland, .... Cumberland, Bee eee wer ee Peewee ewe ree tweens 7a .. |16/4 ... | 21/4 nga aud QUAL 1868|1869) 23/4) 16/4] ... 1870|1871)1872|1873)1874)1875 | ——_$f << — ]———_—_| ————. ] 24/4 25/4 25/4 25/4| 25/4] 25/4| 25/4 26/4| 10/5] 26/4) 29/4 3/5 | 4/5 | 5/5 | 4/5 29/4| 15/4| 24/4] 21/4 19/4 18/4 ‘ o a ea 22/4 GLASGOW SOCIETY OF FIELD NATURALISTS. NIGHTINGALE (Philomela luscinia). 145 1864|1865}1866|1867|1868/1869|1870|1871)1872|1873)1874)1875 Ipswich,.. SE a lees ae Pree een See 19/4. Bromiley, ......... iy! ae sea get es | acc Plates | gece to cehee I Goan Neccmen inact 20) Barnsley, ......... w. |... | 29/4! 28/4) 30/4) 26/4) 1/5 | 27/4] 26/4| 29/4) 1/5 | 9/5 Bradford-on-Avon! ... | . Arca Ges leet atoeee lohaiaat iI cera Seana IPE Sta lear AO Bagshot, .......... | a. |... | 20/4} 18/4] 13/4] 15/4| 15/4! 15/4] 17/4] 17/4] 19/4 Kent, . |... | 20/4] 18/4] 17/4] 23/41 14/4] ....| ... | 12/4] 15/4] 24/4) 21/4 Middlesex, . Bede 20/4 18/4| 21/4) ... saab tevaiafmadeleties BuackeaP (Curruea atricapilla). 1865|1866|1867/1868|1869|1870)1871)1872 1873|1874)1875 Miamsfield,. ....2:0.0.00 sie sis 6/5 Masham,"......... .. AS Se Al eee etl aden 2/5 IBEUSIEY © las ccen . 21/4| 16/4) 15 WHINCHAT (Saxicola rubetra). | 1863 Middlesex, IKenbaetec vucvetce Somerset, .. INObtS hate vena Derbyshire, Wales, . Westmoreland,.. Cumberland, . | 2/4) 16/4 22/4 26/4) aja Se 1864|1865|1866)1867|1868|1869|1870|1871/1872|1873|1874| 1875 18/4 ba. 17/4 ‘a 15/4 24/4) 21/4| 21/4 iad un 31/3 26/4| 26/4| 26/4) 19/4 .. |26/4| 19/4 pee le Dep 24/4| 23/4] 25/4| 19/4 = 11/4 ae 17/4 ek 14/4 Es 30/4 a 14/4 i 2/5 5 27/4 14/4] 14/4] 14/4) 19/4 44) 23/4 19/4 a 28/4 26/4| 28/4] 25/4| 23/4 29/4) 29/4| 29/4| 29/4 1 |1/5 |1/5 | 26/4 4) 25/4) 26/4). ". (27/4 5/5 |2/5 |... ee 21/4 1864|1866|1867|1868|1869|1870 187 1|1872'1873|1874|1875 27/4 ie a. 20/4) . 23/4 + 2/4 n/a | 29/4 25/4) ... | 25/4] 23/4| ... | 21/4 Soh. DjAlicaeh as 3/5 | 23/4) ... 3/5 | 4/5 GLASGOW SOCIETY OF FIELD NATURALISTS. 147 WiLLtow WReEN (Sylvia trochilus). 1866|1867|1868|1869| 1870 1871|1872)1873|1874|1875 Bromley, Kent, .............. Ee DEE AG Meets opts ares A512 as Mansfield, Notts,............ PAM aah face heat [age MAREN aA tare all com stuf LO] Ci Soe een vee | vee | cee Vane [eee | 28/4] 4/5 | 26/4) 2/5 Fochabers, ...........00.0-.-. ... |... | 25/4) 25/4| 25/4| 25/4] 25/4) 25/4] 25/4. LUISE Rear Ane nae Bed ute al lee ee Nii beae We .. | 19/4 OS ee eee eee ee 12/4) 9/4 | 16/4) 11/4) ... 5/4 1/4 a 21/4 BMAGHOROX, 05nd eccene accsie- BAG) OM NERY be lea ap ae Proc lie POMICESCD, Vas se.es...ceeeceoces a 11/4) 12/4 12/4 3 Derby, ae Nae 3/4 Me: ero) ss< Swedes col vas eee ted Beat ie cal ee LC Cumberland, .... 5.000. ss00- ; 23/4) 11/4) ... Curr Cuarr (Sylvia rufa). 1869|1870}1871)1872)1873 18741875 1864/1865)1866|1867/1868 PMeMOU VIMO | ccc. Bes | aa, | voor Loe ... | 20/3 Barnsley, ........ 2/4 | 2/4 | 2/4 | 2/4 2/4 2/4 a4 2/4 zt 2/4. | 15/4 Worcestershire, meal |Meat »| a. [31/3 Mansfield, Notts,| ... nae Hasse diode He CS 9/4 | ... | 31/3) 30/3) 10/4} ... 27/3] 1 5/3 Hovingham, ..... 30/3) 30/3] 30/3] 30/3 30/8 ee le 30/3 30/3 30/3 fd Mocham,:..:...... Boulter y| | eacalieres Icey 19/4 Corwen... ae 9/4 13/4 16/4 te Somerset, . hs 7/4 2/4 23/3 ine Bromley, 27/3/62 si sae Mg bs an | 7/4 25/3| 8/4 | ... | 11/4] ... |23/3 188 21/8 25/8 20/3 Middlesex, antes ey eel dye Ala see Wo aoe ; BEANUS,(. soccee sense |S/4 |... Lys ia ase foe BERD) once. snscs “a 9/4 Roatan TRG, is cis ac as as aaa ... | 6/4 Cumberland,..... ae 11/4) ... SEDGE WARBLER (Salicaria phragmitis). 1865] 1866|1867|1868/1869|1870) 1871|1872|1873|1874,1875 BARNSLEY... .ci.c ss ens 24/4) 24/4) 24/4) 24/4) 24/4) 24/4) 24/4) 24/4| 24/4 22/4 WEMSteld,. c..c.sevecres See leet Ret | os CEN ct av I Pactra eect lees roel DH MESA, Salssasce cases arene bial licts cree RP nal ete | a ae 26/4| 17/4] ... | ... | 19/4 10/5] 10/5] ... |... Middlesex, ............. BE Ne sR Ud A cere biicioe an nee ses PIS oles oc vce cigdsoce ever | 23/4 vs ERCIDES) Vales sos mreieieet! ‘ ‘ « Ny . . . gieieterr eel tae 4