LIBRARY UNIVERSITY OF CALIFORNIA e/I^i^lon ho BIOLOGY ,, i8g y / . Cla&s No. A TEXT-BOOK OF PHYSIOLOGICAL CHEMISTRY. BY OLOF IJAMMARSTEJST, PROFESSOR OF MEDICAL AND PHYSIOLOGICAL CHEMISTRY IN THE UNIVERSITY OF UPSALA. ^ttt{r0rijt& Cranslatbn FROM THE SECOND SWEDISH EDITION AND FROM THE AUTHOR'S ENLARGED AND REVISED GERMAN EDITION BY JOHN A. MANDEL, ASSISTANT TO THE CHAIR OF CHEMISTRY, ETC., IN THE BELLEVUE HOSPITAL MEDICAL COLLEGE AND IN THE COLLEGE OF THE CITY OF NEW YORK. FIRST EDITION. FIRST THOUSAND. YORK: JOHN WILEY & SONS, 53 EAST TENTH STREET. 1893. ROBERT DRUMMOND, Electrot yper, 444 & 446 Pearl Street, New York. Copyright, 1893, BY JOHN A. MANDEI*. &ttj 7, sj BIOLOGY LIBRARY THIS TRANSLATION 18 RESPECTFULLY DEDICATED TO a, 1L1LJ&., WHO GAVE THE IMPETUS TO THE. STUDY OF PHYSIOLOGICAL CHEMISTRY BY FOUNDING THE FIRST CHEMICAL LABORATORY IN CON- NECTION WITH A MEDICAL COLLEGE IN THIS COUNTRY, BY THE TRANSLATOR. UFI7BRSITY PREFACE TO THE GERMAN EDITION. AFTER the appearance of the first Swedish edition of this text- book, I was asked by several co-laborers abroad to provide a German translation, which was at that time impossible, for several reasons. But I found it very difficult to decline a similar proposal which I received from many colleagues after the second edition appeared. I yielded, therefore, to their expressed wishes ; but I found after a time that it was impossible to obtain a translator in this special province of science, notwithstanding the unwearied exertions of my publisher. Nothing remained for me but to undertake the trans- lation myself ; hence I ask the reader's indulgence for possible idiomatic or orthographic errors. Specialists will at once perceive that the book before us is not a complete or detailed text-book. My intention was merely to supply students and physicians with a condensed and as far as pos- sible objective representation of the principal results of physiologico- chemical research and also with the principal features of physio- logico-chemical methods of work. It seems to me that I have followed a common, practical, even if not strictly correct usage in allowing space in this book to the more important pathologico- chemical facts, although I have given the book the title Text- book of Physiological Chemistry. The arrangement of subject-matter, which deviates considerably from that generally followed in text-books, was caused by the manner in which physiological chemistry is studied in Sweden. Here physiologico- and pathologico-chemical laboratory practice is obligatory for all students of medicine. In the arrangement of such practical work I continually kept in view that it should not VI PREFACE TO THE G SUM AN EDITION. consist of isolated, purely chemical or analytico-chemical problems, but that always, as far as possible, it should go hand in hand with the study of the different chapters of chemical physiology. The study of physiologico-chemical processes within the animal body must precede the study of its component parts, its fluids and tissues; and this latter study, according to my experience, will then only inspire true interest if the study of the physiological signifi- cance of those component parts be closely pursued in connection with that of the transformations which take place in these fluids and tissues. In view of this arrangement of subject-matter, and in order to render my book of greater interest and utility to those who do not wish to take cognizance of its analytico-chemical part, I have dis- tinguished the latter by different setting of the type. With the excep- tion of urinary analysis, which practically is of particular impor- tance and which has been treated somewhat elaborately, this part in general depicts only the main points in the methods of preparation and of analytical methods. The instructor who superintends the laboratory practice and who chooses the problems for work has ample opportunity to give the beginner the necessary advanced directions, and for the more experienced student, as well as for the specialist, the excellent works of HOPPE-SEYLER, NEUBAUER-HUP- PERT, and others render more explicit directions superfluous. HAMMARSTEtf. UPBALA, October, 1890. TRANSLATOR'S PREFACE. KNOWING the demands of the medical student and practising physician for a more extended knowledge of physiological chem- istry, and at the same time knowing the lack of literature on this subject in the English language, I have been led to make a translation of this most admirable work. The subject of physiological chemistry is being more and more advanced in this country, until it will soon become an obligatory study in our medical schools, and the enlargement of the literature on the subject will greatly help its progress. It will be seen at a glance that the work is well suited as a laboratory book, for it contains the best methods for the prepara- tion, detection, and quantitative estimation of most of the substances found in the organism and its excretions and secretions. At the author's request I have made no additions or changes whatsoever in the manuscript, and it may appear that some of the methods de- scribed, especially on the urine, are too lengthy and troublesome for the practising physician; still the quick or clinical methods are well described in smaller hand-books on the subject. In the work of translation I have adhered as closely as possible to the author's enlarged German edition and also the original Swedish edition, and therefore the literary errors will perhaps be pardoned. I must here express my appreciation to Mon. A. BOUKGOUGNON who has kindly gone carefully over the manuscript and read the proof-sheets. NEW YORK, October, 1893. UKIYSRSITT CONTENTS. CHAPTER I. PAGE INTRODUCTION 1 CHAPTER II. THE PROTEIN SUBSTANCES 13 CHAPTER III. THE ANIMAL CELL 41 CHAPTER IV. THE BLOOD 54 CHAPTER V. CHYLE, LYMPH, TRANSUDATIONS AND EXUDATIONS 117 CHAPTER VI. THE LIVER 135 CHAPTER VII. DIGESTION 167 CHAPTER VIII. TISSUES OF THB CONNECTIVE SUBSTANCE .233 CONTENTS. CHAPTER IX. PAGE MUSCLE. 251 CHAPTER, X. BRAIN AND NERVES 273 CHAPTER XI. ORGANS OF GENERATION " . 285 CHAPTER XII. MILK 300 CHAPTER XIII. THE SKIN AND ITS SECRETIONS A 322 CHAPTER XIV. THE URINE 330 CHAPTER XV. THE EXCHANGE OP MATERIAL 435 IWITIRSITr PHYSIOLOGICAL CHEMISTRY. CHAPTER I. INTRODUCTION. IT follows from the law of the conservation of force and matter that living beings, plants and animals, can neither produce new matter nor new force. They are only called upon to appropriate and assimilate already existing material and to transform it into new forms of force. Out of a few relatively simple combinations, especially carbon dioxide and water, together with ammonium compounds or nitrates, and a few mineral substances, which serve as its food, the plant builds up the extremely complicated constituents of its organism, albumins, carbohydrates, fats, resins, organic acids, etc. The chemical work which is performed in the plant must therefore, in the majority of cases, consist in syntheses; but besides these, processes of reduction take place to a great extent. The vis viva of the sunlight induces the green parts of the plant to split off oxy- gen from the carbon dioxide and water, and therefore the chief constituents of the plant contain less oxygen than the material serv- ing as food. The vis viva of the sun, which produces this splitting, is not lost ; it is only transformed into another form of force, into the potential energy or chemical tension of the free oxygen on the one side and the combinations less oxygenated produced by the synthesis, on the other side. These conditions are not the same in animals. They are de- pendent either directly, as the herbivora, or indirectly, as the car- 2 PHYSIOLOGICAL CHEMISTRY. nivora, upon plant-life, from which they derive the three chief groups of organic nutritive matter — proteids, carbohydrates, and fat. These bodies, of which the protein substances and fat form the chief mass of the animal body, undergo within the animal organism a splitting and oxidation, and yield as final products ex- actly the above-mentioned chief components of the nutrition of plants, namely, carbon dioxide, water, and ammonia derivatives, which are rich in oxygen and have a feeble chemical tension. The chemical tension, which is partly combined with the free oxygen and partly stored up in the above-mentioned more complex chemi- cal compounds, is transformed into vis viva, heat, and mechanical work. While in the plant reduction processes and syntheses, which are active in the conversion of living force into potential energy or chemical tension, are the prevailing forces, we find in the animal body the reverse of this, namely, splitting and oxidation processes, which convert chemical tension into living force (vis viva). This difference between animals and plants must not be over- rated, nor must we consider that there exists a sharp boundary-line between the two. This is not the case. There are not only lower plants, free from chlorophyll, which in regard to chemical processes represent intermediate steps between higher plants and animals, but the difference existing between the higher plants and animals is more of a quantitative than a qualitative kind. Plants require oxygen as peremptorily as do animals. Like the animal, the plant also, in the dark and by means of those parts which are free from chlorophyll, takes up oxygen and eliminates carbon dioxide, while in the light the oxidation processes going on in the green parts are overshadowed or hidden beneath the more intense reduction pro- cesses. Like the animal the vegetable ferments transform chemi- cal tension into living energy and heat; and even in a few of the higher plants — as the aroidece when bearing fruit — a considerable development of heat has been observed. The reverse is found in the animal organism, for, besides oxidation and splitting, reduction processes and syntheses also take place. The contrast which seemingly exists between animals and plants consists merely in that in the animal organism the processes of oxidation and split- ting are prevalent, while in the plant those of reduction and syn- thesis have mostly been observed. INTRODUCTION. 3 WOHLER iii 1824 furnished the first example of SYNTHETICAL PROCESSES within the animal organism. He showed that when benzoic acid is introduced into the stomach it reappears as hippuric acid in the airine^ after its coupling with glycocoll (amido-acetic acid). Since the discovery of this synthesis, which may be ex- pressed by the following equation, C6H6.COOH + NH2.CHa.COOH=NH(C6H6.CO).CH9.COOH+HaO, Benzoic acid Glycocoll Hippuric acid and which is ordinarily considered as a type of an entire series of syntheses occurring in the body where water is eliminated, the number of known syntheses in the animal kingdom has increased considerably. Many of these syntheses have also been artificially produced outside of the organism, and numerous examples of ani- mal syntheses of which the history is absolutely clear will be found in the following pages. Besides these well-studied syntheses, there occur in the animal body also similar processes unques- tionably of the greatest importance to animal life, but of which we know nothing with positiveness. We enumerate as examples of this kind of synthesis the new formation of the red blood-corpus- cles (the haemoglobin), the formation of the different albumins from the peptones, the formation of fat from carbohydrates, and others. The chemical processes in the animal body we have mentioned above as consisting chiefly of oxidation and splitting processes. The oxygen of inhaled air, as also that of the blood, is now called neutral, molecular oxygen, and the old assumption that ozone occurs in the organism has now been discarded for several reasons. There are few substances which can be introduced from the outside, such as aldehydic compounds and certain alcohols, for example benzyl- alcohol (SCHMIEDEBERG), which can be oxidized within the animal organism by the neutral oxygen; while, on the contrary, albumin and fat, which form the chief part of the organic constituents of the animal body, are almost indifferent to neutral oxygen. The question arises, how then is the oxidation of these and other bodies possible in the animal organism ? Formerly the view was generally accepted that ANIMAL OXIDA- TION took place in the animal fluids, while to-day we are of the 4 PHYSIOLOGICAL CHEMISTRY. opinion that it is connected with the form-elements and the tis* sues. The question how this oxidation in the form-elements pro- ceeds and how it is induced cannot be answered with certainty. In conformity with the views of PFLUGER and others, it is often asserted that the albumin outside of the organism, and also that which circulates in the blood and fluids, is to be regarded as " dead albumin," as distinguished from that which is converted by the work of the living, active cell into living protoplasm — "living albumin/' The statement has also been made that this living protoplasm albumin is differentiated from the "dead albumin "by a greater mobility of the atoms within the molecule, and it may be char- acterized by a greater inclination towards intramolecular changes of position of these atoms. The reason for this greater inner movement PFLUGER ascribes to the presence of certain groups, such as cyanogen, while LOEW attributes it to the presence of alde- hydic groups in the albumen molecule. In these differences between ordinary albumins and living protoplasm albumin PFLUGER sees the reason for the animal oxi- dation processes which show certain similarity to the oxidation of phosphorus in air containing oxygen. In the last-mentioned process the phosphorus is not only itself oxidized, but, as it splits the oxygen molecule and sets free oxygen atoms (active oxygen), it may also have at the same time an indirect or secondary oxidizing action upon other bodies present. In an analogous way the living protoplasm albumin, which is not, like the dead albu- min, indifferent to neutral oxygen, can disintegrate the oxygen molecule, thus becoming itself oxidized, and at the same time the setting free of oxygen atoms may cause a secondary oxidation of other less oxidizable substances. Another very widely-diffused view exists in regard to the origin of the activity of the oxygen, so that by the decomposition pro- cesses in the tissues reducing substances are formed which split the neutral oxygen molecule, uniting with one oxygen atom and setting the other free. The formation of reducing substances by fermentation and putrefaction is generally known. The butyric fermentation of sugar in which hydrogen is set free — C6H1206 = C,H802 -f- 2C02 -j- 2(H2) — is an example of this kind. Another example is the INTRODUCTION. 6 appearance of nitrates in consequence of an oxidation of nitrogen in cases of putrefaction, which process is ordinarily explained by the statement that, in putrefaction, reducing, easily-oxidizable bodies are formed which split oxygen molecules, liberating oxygen atoms which afterward oxidize the nitrogen. It is assumed also that the cells of the animal tissues and organs, like these lower organ- isms, which cause fermentation and putrefaction, undergo such splitting processes in which easily-oxidizable substances, perhaps also hydrogen in statu nascendi (HOPPE-SEYLER), are produced. The observations of EHRLICH, that certain blue coloring matters — alizarin blue and indophenol blue — are decolorized by the tissues of the living animal and become blue again on exposure to air, seem also to be a proof of the occurrence of easily-oxidizable combina- tions in the tissues. A further proof of this is found in the obser- vations of C. LUDWIG and ALEX. SCHMIDT that in the blood of asphyxiated animals, as well as in the absence of oxygen, an accu- mulation of reducing, easily-oxidizing substances takes place. In accordance with what has been above stated, we may assume that the oxidation in the animal body takes place in the following manner: The forces peculiar to protoplasm, jinknown to us, but acting similarly to heat, increase the intramolecular movements of the atoms in such a. way that a loosening or splitting of the molecule occurs and an aggregation of the oxygen is made possible ("primary oxidation/' NASSE). The new products formed in this manner may perhaps in part be in direct combination with neutral oxygen (" direct oxidation/' NASSE) and gradually burned within the body, but they must probably first undergo a further splitting, and then succumb to consecutive oxidation, until, after repeated splitting and oxidation, the final products of the exchange of material are formed. Finally, the easily-oxidizable products of decomposition, when they split the oxygen molecule and only combine with one of the oxygen atoms, may act on difficultly- oxidizable substances in an indirect or, as NASSE has called it, a "secondary oxidation" by the setting free of the second atom. Thus the oxidation within the animal body is caused by the action of forces acting similarly to heat, which loosens or splits the molecules; and since this oxidation has long been known as com- bustion, this view is easily reconcilable with the mode of action UFITBRSITT 6 PHYSIOLOGICAL CHEMISTRY. just described. In combustion in the ordinary sense, as, for example, the burning of wood or oil, we must not forget that the substances themselves do not combine with oxygen. It is only after the action of heat has decomposed these bodies to a certain degree that the oxidation of the products of such decom- position takes place and is accompanied by the phenomenon of light. The numerous intermediary products of decomposition which we observe in the animal body teach us that the oxidations and splittings of the components of the body do not take place at once and suddenly, but only very gradually, step by step, until the final products of exchange are reached. A very instructive example, of such a gradual decomposition outside of the organism has been shown by DKECHSEL in his in- vestigation on the electrolysis of phenol by an alternating current. By experiments with alternating electric currents we obtain, of course, in the watery solution of the substance, at each electrode alternately, oxygen and hydrogen in great rapidity. Therefore oxidations and reductions must take place alternately, and we ob- tain syntheses as well as splittings with oxidations. If phenol in watery solution is treated with such an alternating current, we produce, by the combined action of reduction and oxidation processes, where all double linking in the benzol ring is broken by the aggregation of hydrogen atoms with simultaneous solution, and followed by an oxidation with the elimination of hydrogen atoms, a new body, hydro-phenoketon, CeH100, or OH, TT P/ \Pf) I 0' 'OH * From *ke hydro-phenoketon a compound of the 1 X fatty series is produced by the fixation of 0 -f- 2H accompanied with the splitting of the benzol ring, namely, normal caproic acid, OH, O.H.A, or H,C| JcH°H- By further electrolysis of the ca- ' CH, proic acid, with the removal of carbon as carbon dioxide and of INTRODUCTION. 7 hydrogen as water, a series of acids with decreasing amounts of carbon are obtained, and in this way we may, by properly directed combination of reductions and oxidations, pass from a body of the aromatic series to a body of the fatty series, and then to substances in which the amount of carbon decreases, until the final products of animal exchange are reached. That reduction processes occur in the organism has already been stated, and in the following pages special examples of these are given. As DRECHSEL has also found that the same electro-syn- theses (of urea and phenol-sulphuric acid) are produced by the continuous as well as by the alternating current, and since the occurrence of galvanic currents in the body has been positively shown, DRECHSEL concludes that not only do syntheses, but also the combustion of foods and constituents of the tissues, take place in the animal body in consequence of a quick succession of reduc- tions and oxidations. Another attempt to explain the animal oxidations has been made by M. TRAUBE. TRAUBE has brought forward powerful arguments against the view that an activity of the oxygen is caused by reducible substances, and he seems to believe that within the organism so-called oxygen transmitters occur which act similarly to nitric oxide in the manufacture of sulphuric acid where oxida- tion is the result of the absorption and liberation of oxygen by other substances which are themselves not directly oxidized by neutral oxygen. The presence of such bodies in the animal organ- ism has not thus far been proved. An important source of the vis viva developed in the body is to be sought for in the oxidation derived from oxygen of strong poten- tial energy, but also in the splitting processes ; where more com- plicated chemical compounds are reduced to simpler ones, and when, therefore, the atoms change from a movable equilibrium to a stabler one and stronger chemical affinities are satisfied, the chem- ical potential energy is transformed into vis viva (or living energy). The best-known example of such a splitting process outside of the animal organism is the ordinary alcoholic fermentation of sugar, C6H1206 = 200, -f 2C2H60, in which process heat is set free. The animal body may also have a source of vis viva in the splitting processes which are not dependent on the presence of free oxygen. 8 PHYSIOLOGICAL CHEMISTRY. The processes taking place in the living muscle yields an example of this kind. A removed muscle, which gives no oxygen when in a vacuum, may, as HERMANN has shown, work, at least for a time, in an atmosphere devoid of oxygen, and give off carbon dioxide at the same time. We call processes of splitting which are accompanied by a de- composition of water and then a taking up of its constituents (H3 and 0) hydrolytic splittings. These splittings, which play an im- portant role within the animal body, and which are most frequently met with in the process of digestion, are, for example, the trans- formation of starch into sugar and the splitting of neutral fats into the corresponding fatty acid and glycerin. (^.(C..^/).). + 3 H,0 = 0,H6(OH), + 3(CISHS60,). Tristearin. Glycerin. Stearic acid. As a rule the hydrolytic splitting processes as they occur in the animal body may be performed outside of it by means of higher temperatures with or without the simultaneous action of acids or alkalies. Considering the two above-mentioned examples, we know that starch is converted into sugar when it is boiled with dilute acids, and also that the fats are split into fatty acids and glycerin on heating them with caustic alkalies or by the action of super- heated steam. The heat of the chemical reagents which are used for the performance of these reactions would cause immediate death if applied to the living system. Consequently the animal organism must have other means at its disposal which will act similarly, but in such a manner that they may work without endangering the life or normal constitution of the tissues. Such means have been recognized in the so-called formless ferments or enzymes. Alcoholic fermentation, as well as other processes of fermenta- tion and putrefaction, is dependent upon the presence of living organisms, ferment fungi and splitting fungi of different kinds; and according to the researches of PASTEUR, these processes are to be considered as phases of life of these organisms. The name orga- nized ferments OT ferments has been given to such micro-organisms of which ordinary yeast is an example. However, the same name has also been given to certain bodies or mixtures of bodies of unknown INTRODUCTION. 9 organic origin which are products of the chemical work within the cell, and which, after they are separated from the cell, are capable in the smallest quantities of causing a decomposition or splitting in very considerable quantities of other substances with- out entering into combination with the decomposed body or with any of its products of splitting or decomposition. Such ferments are, for example, the diastase of malt and the ferments secreted by the different glands participating in the process of digestion. These non-organized or formless ferments are generally called, ac- cording to KUHNE, enzymes. A ferment in a more restricted sense is therefore a living being, while an enzyme is a product of chemical processes in the cell, a product which has an individuality even without the cell, and which may be active when separated from the cell. The splitting of glucose or invert-sugar into carbon dioxide and alcohol by fer- mentation is a fermentative process closely connected with the life of the yeast. The inversion of cane-sugar is, on the contrary, an enzymotic process caused by one of the bodies or mixture of bodies formed by the living ferment, which can be severed from this fer- ment, and still remains active even after the death of the latter. Consequently ferments and enzymes are capable of manifesting a different behavior towards certain chemical reagents. Thus there exist a number of substances, among which we may mention arse- nious acid, phenol, salicylic acid, boracic acid, chloroform, ether, and others, which in certain concentration kill ferments, but which do not noticeably impair the action of the enzymes. It is doubtful, indeed highly improbable, whether it has been possible up to the present time to isolate any enzyme in a pure state. Therefore the nature of the enzymes and their elementary compo- sition is unknown. Such as have been obtained thus far appear to be nitrogenized and to be similar in some degree to albuminous bodies. They may be extracted from the tissues by means of water or glycerin, especially by the latter, which forms very stable solu- tions and which, consequently, serves as a means of extraction of the enzymes. The enzymes, generally speaking, do not appear to be diffusible, they decompose hydrogen peroxide and are precipi- tated with other substances when these are in a finely-divided state. This property has also often been taken advantage of in the prepa- 10 PHYSIOLOGICAL CHEMISTRY. ration of pure enzymes. The continued heating of their solutions above + 80° C. generally destroys most of the enzymes. In the dry state, however, certain enzymes may be heated to 100° or indeed to 150°-160° C. without losing their power. The enzymes are pre- cipitated from their solutions by alcohol. We have no characteristic reactions for the enzymes in general, and each enzyme is characterized by its specific action and by the conditions under which it develops. But it must be stated that, however the different enzymes may vary in action, they all seem to have this in common, that by their presence an impulse is given to split more complicated combinations into simpler ones, whereby the atoms arrange themselves from an unstable equilibrium into a more stable one, chemical tension is transformed into living force, and new products are formed with lower heat of combustion than the original substance. The presence of water seems to be a necessary factor in the perfection of such decompositions, and the chemical process seems to consist in the taking up of the elements of water. The manner in which these enzymes work is still enveloped in darkness, but their action may be considered as very closely related to the so-called catalytic or contact action. As above stated, the enzymes are of great importance for the chemical processes going on in the digestive tract, but we have to add that the results of their action is greatly complicated by pro- cesses of putrefaction which take place in the intestines at the same time, and which are caused by micro-organisms. Micro-organisms are physiological constituents of the contents of the intestinal canal, and it is therefore to be supposed that also lower organisms or their germs are to be encountered in the animal tissues and fluids gen- erally, under normal conditions. The question is still unsettled as to how far this is the case. But as yet no positive or decisive proof for the justification of such a statement has been furnished. The lower organisms, on the contrary, when they enter into the animal fluids or tissues and develop and increase, are of the greatest patho- logical importance, and modern bacteriology, founded by KOCH and PASTEUE, in relation to the doctrine of infectious diseases, gives efficient testimony to these facts. Putrefaction caused within the animal fluids and tissues by the lower organisms may produce, among others, combinations of a INTROD UCTION. 1 1 basic nature* Such bodies were first found by SELMI in human cadavers, and called by him cadaver alkaloids or ptomaines. These ptomaines, which have been studied by a great number of investi- gators, especially by SELMI, BEIEGER, and GAUTIEE, must be con- sidered as products of chemical processes caused by putrefaction microbes. Some of these ptomaines are exceedingly poisonous, and consequently BRIEGER has called them toxines. The formation of such poisonous products in the decompositions caused by putrefactive microbes makes it probable that the lower organisms acting in infectious diseases also produce poisonous sub- stances which may cause by their action the symptoms or compli- cations of the disease. BRIEGER, who has become prominent by his study of this subject, has been able to isolate from typhus cultures a substance called typhotoxin which has a poisonous action on animals; and he has also prepared another substance, tetanin, from the amputated arm of a patient with tetanus, animals- inocu- lated with which die exhibiting symptoms of developed tetanus. Admitting that the results obtained thus far upon this subject are not very numerous, we cannot refrain from stating that the facts al- ready found open a promising field for further labor and research. As above stated, the chemical processes in animals and plants do not stand in opposition to each other; they offer differences indeed, but still they are of the same kind from a qualitative standpoint. PFLUGER says that there exists a blood-relationship between all living cells of the animal and vegetable kingdoms, and that they originate from the same root ; and if the organisms consisting of one cell can decompose protein substances in such a manner as to pro- duce poisonous substances, why should not the animal body, which is only a collection of cells, be able to produce under physiological conditions similar poisonous substances ? The poisonous secretions of toads, serpents, and numerous other animals prove, in fact, that the animal body has this power. Frequent efforts have been made of late to prove that the human organism under physiological con- ditions produces poisonous substances. In the human saliva (GAU- TIER and others), and especially in the urine (POUCHET, BOUCHARD, and others), but also in the expired air (BROWN-SEQUARD and D'ARSONVAL), poisonous organic substances have been proved to exist. The correctness of many of these statements is contradicted 12 PHYSIOLOGICAL CHEMISTRY. by other investigators, and the interesting question as to the poison- ous nature of the human physiological secretions and excretions seems to require further proof. The so-called leucomaines command a special interest. Those substances of basic nature which are incessantly and regularly pro- duced as products of the decomposition of the protein substances of the organism, and which therefore are to be considered as products of the physiological exchange of substance, have been called leuco- maines by GrAUTiER in contradistinction to the ptomaines produced by micro-organisms. These substances, with the exception of a few which were known in animal extractives, were first found by GAUTIER in animal tissues and muscles, and among these he finds some which are poisonous in small doses. The leucomaines of late are considered of special importance as originators of disease. It has been contended that when these bodies accumulate, on ac- count of an incomplete excretion or oxidation in the system, an autointoxication may be produced (BOUCHARD). Though this view is not by any means based upon generally-recognized facts, still it oilers an interesting starting-point for physiological and pathological chemical research. CHAPTER II. THE PROTEIN SUBSTANCES. THE chief mass of the organic constituents of animal tissues consists of amorphous, nitrogenized, complex bodies of high molecu- lar weight. These bodies, which are either albuminous in a special sense or bodies nearly related thereto, take first rank among the organic constituents of the animal body on account of their great abundance. For this reason they are classed together in a special group which has received the name protein group (from TtpGorevco, I am the first or take the first place). The bodies belonging to these several groups are called protein substances, although in a few cases the albuminous bodies in a special sense are designated by the same name. The several protein substances contain carbon, hydrogen, nitro- gen, and oxygen. They generally contain also sulphur, a few phosphorus, and a few also iron. Copper has been found in some few cases. On heating the protein substances they gradually de- compose, producing inflammable gases, ammoniacal compounds, carbon dioxide, water, nitrogenized bases, as well as many other bodies, and at the same time they emit a strong odor of burnt horn or wool. More highly heated they leave a porous, shining mass of carbon, and when this is thoroughly burnt an ash is obtained con- sisting chiefly of calcium and magnesium phosphates. The ques- tion whether the mineral bodies left by burning exist as impurities or whether they are constituents of the protein molecule has not been decided. It is at present impossible to decide on a classification of the protein substances based upon their properties, reactions, and con- stitution, as well as upon their solubilities and precipitations, corre- sponding to the demands of science. The best classification is perhaps the following systematic summary of the better known 13 14 PHYSIOLOGICAL CHEMISTRY. and studied animal protein substances, due mainly to HOPPB- SEYLEE and DEECHSEL. I. Albuminous Bodies. Serum albumin, Albumins •{ Ovalbumin, Lactalbumin. Serum globulin, Fibrinogen, Myosin, Musculin, Vitellin (?). Casein, Ovovitellin (?), Pyin, and others. j Acid albuminate, \ Alkali albuminate. Alb urn oses and Peptones. Fibrin, Albumin coagulated by heat, and others. Globulins Nucleoalbumins Albuminates . . . Albumoses a Coagulated . Mucine II. Proteids. Pure Mucin, Mucoide or Mucinoide. (Hyalogen.) Haemoglobin. *II. Albumoides or Albuminoides. Keratine. Elastin. Collagen. (Amyloid.) (Fibroin, Sericin, Cornein, Spongin, Conchiolin, tfyssus, and others.) To this summary must be added that we often find in the in- vestigations of animal fluids and tissues protein substances which t ' THE PROTEIN SUBSTANCES. do not coincide with the above scheme or do so only with difficulty. At the same time it must be remarked that bodies will be found which seem to rank between the different groups. I. Native Albumins. The albuminous bodies are never-failing constituents of the animal and vegetable organisms. They are especially found in the animal body, where they form the solid constituents of the muscles, glands, and the blood serum, and they are so generally distributed that there are only a few animal secretions and excretions, such as the tears, perspiration, and perhaps urine, in which they are en- tirely absent or only occur as traces. All albuminous bodies contain carbon, hydrogen, nitrogen, oxy- gen, and sulphur; a few contain also phosphorus. Iron is generally found in traces in their ash, and it seems to be a regular constitu- ent of a certain group of the albuminous bodies, namely, the nucleo-albumin group. The composition of the different albu- minous bodies deviates a little, but the variations are within rela- tively close limits. For the better studied animal albuminous bodies the following conposition of the ash-free substance has been given: c . . . , . . 506 — 54 5 per cent H . . . . ... 6.5 — 7.3 " N . . . , . . . 15 0 — 17 6 " S . . . . ... 0.8 — 2.2 " P . 0.42 — 0.85 " 0 21.50 -- 23.50 " A part of the nitrogen of the albumin molecule is loosely com- bined and splits off easily as ammonia by the action of alkalies (NASSE). Sulphur shows the same property in nearly all albu- minous bodies (FLEITMANN, DANILEWSKT, KRUGEE). A part of the sulphur separates as potassium or sodium sulphide on boiling with caustic potash or soda, and may be detected by lead acetate. What remains can only be detected after fusing with nitre and sodium carbonate and testing for sulphates. The albumin molecule therefore contains at least 2 atoms of sulphur. The molecular 16 PHYSIOLOGICAL CHEMISTRY. weight of the albumins has not been determined, therefore it is impossible to give formulae. For the alkali albuminate, in whose formation from native albumins a part of the nitrogen and the loosely-bound sulphur is split off, LIEBERKUHN has given the formula CtlH11QN18SOM. The constitution of the albuminous bodies, notwithstanding numerous investigations, is still unknown. By heating albumin with barium hydrate and water in sealed tubes at 150°-200° C. for several days, SCHUTZENBERGER obtained a number of products among which were ammonia, carbon dioxide, oxalic acid, acetic acid, and, as chief product, a mixture of amido-acids. This mix- ture contained, besides a little tyrosin and a few other bodies, chiefly acids of the series CnH3n+1N02 (leucines) and CnH2n_iNOa (leuceines). The sulphur of the albumins yields sulphites. The three bodies, carbon dioxide, oxalic acid, and ammonia, are formed in the same relative proportion as in the decomposition of urea and oxamid; therefore 'SCHUTZENBERGER suggests that perhaps albumin may be considered as a very complex ureid or oxamid. Such a conclusion cannot be derived from the above decomposition processes for several reasons, and the attempts to prepare urea directly by splitting albumin by means of trypsin, or by oxidation, have given negative, or at least not positive, results. On fusing albumin with caustic alkali, ammonia and other volatile products are generated; among these leucin, from which volatile fatty acids, such as acetic acid, valerianic acid, and also butyric acid, are formed; also tyrosin, from which phenol, indol, and skatol are produced. The majority of these products are found as a result of putrefaction (see Chap. VII). On boiling with mineral acids, or still better by boiling with hydrochloric acid and zinc chloride (HLASIWETZ and HABERMANIJ), the albumins yields amido-acids, such as leucin, aspartic acid, glutamic acid, and tyrosin (and from vegetable albumin SCHULZE and BARBIERI ob- tained tf-phenylamido-propionic acid), also sulphuretted hydrogen, ammonia, and nitrogenized bases (DRECHSEL). As an essential difference between the action of acids and alkalies (barium hydrate) on albumins, DRECHSEL suggests that by the action of acids car- bon dioxide, oxalic and acetic acids are not produced. By the putrefaction of albumins, as well as by decomposition THE PROTEIN SUBSTANCES. 17 by means of acids or alkalies, and also by certain enzymes, among other products amido-acids are produced, and these have a certain significance for the probable formation of the albumins. It is more than likely that in the synthesis of albumin in the plant from the ammonia or the nitric acid of the soil, amido-acids or acid amids, among which asparagin plays an important role, are pro- duced; and from these the albuminous bodies are derived by the influence of glucose or other non-nitrogenized combinations. By the oxidation of albumins in acid solutions, volatile fatty acids, their alde- hydes, nitriles, ketones, also benzoic acid are obtained, also hydrocyanic acid by oxidizing with potassium dichromate and acid. Nitric acid gives various nitro-products, such as xanthoproteic acid (VAN DEE PANTS), trinitroalbumin (LoEW) or oxynitroalbuinin, nitrobenzoic acid, and others. With aqua regia fumaric acid, oxalic acid, chlorazol, and other bodies are produced. By the action of bromine under stronger pressure a large number of derivatives are obtained, such as bromanil and tribromacetic acid, bromoform, leucin, leu- cinimid, oxalic acid, tribromamido-benzoic acid, peptone, and bodies similar to humus. By the dry distillation of albumins we obtain a large number of decompo- sition products of a disagreeable burnt odor, and a porous glistening mass of carbon containing nitrogen is left as a residue. The products of distillation are partly an alkaline liquid which contains ammonium carbonate and acetate, ammonium sulphide, ammonium cyanide, an inflammable oil and other bodies, and a brown oil which contains hydrocarbons, nitrogenized bases belonging to the aniline and pyridine series, and a number of unknown substances. It is impossible here to discuss all the products obtained by the action of different reagents on the albumins, but from the above- described bodies from the decomposition of albumin it is clear that the products belong in part to the fatty and in part to the aro- matic series. Even though the constitution of the albumins has as yet not been successfully demonstrated, it seems to be a fact from the above that in the albumin molecule we have, besides the atomic arrangement belonging to the fatty series, at least one aromatic group present. By the oxidation of albumin by means of potassium permanganate, MALY obtained an acid, the oxyprotosulphonic acid, C 51.21; H 6.89; N 14.59; S 1.77; 0 25.54, which is not a product of splitting, but an oxidation product in which the- group SH is changed into SO2.OH. This acid does not give the proper color reaction with MILLON'S reagent (see below), nor does it yield the ordinary aromatic splitting products of the albumins. Still the aromatic group is not absent, but it seems to be in another binding from that in ordi- nary albumin. On oxidizing with potassium dichromate and acid this group appears as benzoic acid, and on fusing with alkali, benzol is given off. The animal albuminous bodies are odorless and tasteless, ordi- narily amorphous. The crystalloid (DOTTERPLATTCHEN) occurring 18 PHYSIOLOGICAL CHEMISTRY. in the eggs of certain fishes and amphibians does not consist of pure albumin, but of an albumin containing large amounts of lecithin which seems to be combined with mineral substances. Crystalline combinations of albumin with mineral substances have been prepared from seeds of various plants, and lately crystallized animal albumin in combination with salts has been prepared (HOFMEISTEK). In the dry condition the albuminious bodies ap- pear as a white powder, or when in thin layers as yellowish, hard, transparent plates. A few are soluble in water, others only soluble in salty or faintly alkaline, or acid solutions while others are insol- uble in these solvents. All albuminous bodies when burnt leave an ash, and it is therefore questionable whether there exists an al- buminous body which is soluble in water without the aid of mineral substances. Nevertheless it has not been thus far successfully proved that a native albuminous body can be prepared perfectly free from mineral substances without changing its constitution or its properties. The albuminous bodies are in most cases strong colloids. They diffuse, if at all, only very slightly through animal membranes or parchment-paper, and the albumins have generally a very high osmotic equivalent. All albuminous bodies are optically active and turn the ray of polarized light to the left. On heating a solution of albumin to the temperatures depending on the albumin present, and with the proper reactions and in favorable external conditions, — as, for example, in the presence of neutral salts, — most albuminous bodies separate in the solid state as a crude or " coagulated " albumin. The different temperatures at which the various albuminous bodies coagulate in neutral, salty solutions give in many cases a good means for detecting and sepa- rating these bodies. The general reactions for the albuminous bodies are numerous, but only the most important will be given here. To facilitate the study of these they have been divided into the two following groups. A. Precipitation Reactions of the Albuminous Bodies. 1. Coagulation Test. An alkaline albumin solution does not co- agulate on boiling, a neutral solution only partly and incompletely, and the reaction must therefore be acid for coagulation. The THE PROTEIN SUBSTANCES. 19 neutral liquid is first boiled and then the proper amount of acid added carefully. A flocculent precipitate is formed, and if prop- erly done the filtrate should be water-clear. If dilute acetic acid be used for this test, the liquid must first be boiled and then 1, 2, or 3 drops of acid added, depending on the amount of albumin present, and boiled before the addition of each drop. If dilute nitric acid be used, then to 10-15 c.c. of the previously-boiled liquid 15-20 drops of the acid must be added. If too little nitric acid be added a soluble combination of the acid and albumin is formed which is precipitated by more acid. An albumin solution containing a small amount of salts must first be treated with about \% NaCl, since the heating test may fail, especially on using acetic acid, in the presence of only a slight amount of albumin. 2. Behavior towards Mineral Acids at Ordinary Temperatures. The albumins are precipitated by the three ordinary mineral acids and by metaphosphoric acid, but not by orthophosphoric acid. If nitric acid be placed in a reagent glass and the albumin solution be allowed to flow gently thereon, a white, opaque ring of precipi- tated albumin will form where the two liquids meet (HELLER'S albumin test). 3. Precipitation by Metallic Salts. Copper sul- phate, neutral and basic lead acetate (in small amounts), mercuric chloride, and other salts precipitate albumin. On this is based the use of albumins as antidotes in poisoning by metallic salts. 4. Pre- cipitation by Ferro- or Ferricyanide of Potassium in Acetic Acid Solution. In these tests the relative quantities of reagent, albumin, or acid do not interfere with the delicacy of the test. 5. Precipi- tation by Neutral Salts, such as Na2S04 or NaCl, when added to sat- uration to the liquid acidified with acetic acid or hydrochloric acid. 6. Precipitation by Alcohol. The solution must not be alkaline, but must be either neutral or faintly acid. It must, at the same time, contain a sufficient quantity of neutral salts. 7. Precipita- tion by Tannic Acid in acetic-acid solutions. The absence of neu- tral salts or the presence of free mineral acids may not cause the precipitate to appear, but after the addition of a sufficient quan- tity of sodium acetate the precipitate will in both cases appear. 8. Precipitation by Phospho-tungstic or Phospho-molybdic Acids in the presence of free mineral acids. Potassium-mercuric iodide and potassium-bismuth iodide precipitate albumin solutions acidified 20 PHYSIOLOGICAL CHEMISTS Y. with hydrochloric acid. 9. Precipitation by Picric Acid in solu- tions acidified by organic acids. B. Color Reactions for Albuminous Bodies. 1. Millon's reaction.1 A solution of mercuric nitrate in nitric acid containing some nitrous acid gives a precipitate in albumin solutions which at the ordinary temperature is slowly, but at the boiling-point more quickly, colored red ; and the solution may also be colored a feeble or bright red, depending on the amount of albumin. Solid albuminous bodies, when treated by this reagent, give the same coloration. This reaction, which depends on the presence of the aromatic group in the albumin, is also given by tyrosin and other benzol derivatives , with a hydroxyl group in the benzol nucleus. 2. Xanthoproteic reaction. With strong nitric acid the albuminous bodies give, on heating to boiling, yellow flakes or a yellow solution. After saturating with ammonia or alkalies the color becomes orange- yellow. 3. Adamkiewicz' reaction. If a little albumin is added to a mixture of 1 vol. concentrated sulphuric acid and 2 vols. glacial acetic acid a reddish-violet color is obtained slowly at ordinary temperatures, but more quickly on heating. Gelatine does not give this reaction. 4. Biuret test. If an albumin solution be first treated with caustic potash or soda and then a dilute copper sulphate solution be added drop by drop, first a reddish, then a reddish-violet, and, lastly, a violet-blue color is obtained. 5. Al- bumin is soluble on heating with concentrated hydrochloric acid, producing a violet color, and when the albumin is first boiled with alcohol and then washed with ether (LIEBERMANN) it gives a beautiful blue "solution. 6. With concentrated sulphuric acid and sugar (in small quantities) the albuminous bodies give a beautiful red coloration. These color reactions apply to all albuminous bodies. The delicacy of the same albumin reagent differs for the differ- 1 The reagent is obtained in the following way : 1 pt. mercury is dissolved in 2 pts. of nitric acid (of sp. gr. 1.42), first when cold and later by warming. After complete solution of the mercury, add 1 volume of the solution to 2 volumes of water. Allow this to stand a few hours and decant the super natant liquid. THE PROTEIN SUBSTANCES. 21 ent albuminous bodies, and it is, on this account, impossible to give the degree of delicacy for each reaction for all albuminous bodies. Of the precipitation reactions HELLER'S test (if we eliminate the peptones and certain albumoses) is recommended in the first place for its delicacy, though it is not the most delicate reaction, and because it can be performed so easily. Among the precipitation reactions, that with basic lead acetate (when carefully and exactly executed) and the reactions 6, 7, 8, and 9 are the most delicate. The color reactions 1 to 4 show a great delicacy in the order in which they are given. No albumin reaction is in itself characteristic, and, therefore, in testing for albumin one reaction is not sufficient, but a number of precipitation and color reactions must be employed. For the quantitative estimation of coagulable albumin the determination by boiling with acetic acid can be performed with advantage since, by operating carefully, it gives exact results. The precipitation by means of alcohol in the liquid which has first been neutralized may also be used for this purpose. The alcohol must be added so that the liquid contains 70 to 80 vols. per cent. In both cases small amounts of albumin may remain in the filtrate. This last may be determined by concentrating the filtrate sufficiently (in the alcohol method all the alcohol must be expelled), and remov- ing any separated fat by shaking with ether, and then precipitating with tannic acid, with the addition of NaCl if necessary. Ap- proximately 63$ of the tannic acid precipitate washed with cold water and dried may be considered as albumen. The precipi- tation by means of copper sulphate may also be employed as a quantitative method, while the Biuret reaction may be used for the quantitative calorimetric estimation of peptones and albumoses. The quantitative estimation of albumin by means of the polari- scope is not applicable in all cases and does not give sufficiently exact results. The separation of albumins from a solution may, in most cases, be performed by boiling with acetic acid. Small amounts of albu- min which remain in the filtrates may be separated by boiling with freshly-precipitated lead carbonate (HOFMEISTER) or with ferric acetate (HOPPE-SEYLEE), as described in Chapter XIV on the urine. If the liquid cannot be boiled, the albumin may be pre- cipitated by neutral salts and acid, or by the very careful addition of lead acetate, or by the addition of alcohol. If the liquid con- tains substances which are precipitated by alcohol, such as glycogeu, then the albumin may be separated by the alternate addition of potassium-mercuric iodide and hydrochloric acid (BRUCKE). 22 PHYSIOLOGICAL CHEMISTRY. Synopsis 01 the Most Important Properties of the Different Chief Groups of Albuminous Bodies. Albumins. These bodies are insoluble in water and are not precipitated by the addition of a little acid or alkali. They are precipitated by the addition of large quantities of mineral acids or metallic salts. Their solution in water coagulates on boiling in the presence of neutral salts, but a weak saline solution does not. If NaCl or MgS04 is added to saturation to a neutral solution in water at a normal temperature or at -j- 30° C. no precipitate is formed ; but if acetic acid is added to this saturated solution the albumin readily separates. When ammonium sulphate is added in substance to saturation to an albumin solution a complete pre- cipitation occurs at ordinary temperature. Of all the albuminous bodies the albumins are the richest in sulphur, containing from 1.6 to 2.20. Globulins. These albuminous bodies are insoluble in water, but dissolve in dilute neutral salt solutions. The globulins are precipitated unchanged from these solutions by sufficient dilution with water; and on heating they coagulate. The globulins dis- solve in water on the addition of very little acid or alkali, and on neutralizing the solvent they reprecipitate. The solution in a minimum amount of alkali is precipitated by carbon dioxide, but the precipitate may be redissolved by an excess of the precipitant. The neutral solutions of the globulins contain- ing salts are partly or completely precipitated on saturation with NaCl or MgS04 in substance at normal temperatures. The globu- lins contain an average amount of sulphur, not below 10. A sharp line between the globulins on one side and the artificial albu- rn in ates on the other can hardly be drawn. The albuminates are, indeed, as a rule insoluble in dilute common-salt solutions ; but sin albuminate may be prepared by the action of strong alkali which is soluble in common-salt solutions immediately after precipitation. We also have globulins which are insoluble in NaCl after having been in contact with water for some time. Nucleoalbumins. These bodies are found widely diffused in both the animal and vegetable kingdoms. They form one of the chief constituents of protoplasm, while the albumins and in part also the globulins are special constituents of the animal juices. THE PROTEIN SUBSTANCES, 23 The nucleoalbumins are found in organs abounding in cells, but they also occur in secretions and sometimes in other fluids in appa- rent solution as destroyed and altered protoplasm. The nucleo- albumins behave like rather strong acids; they are nearly insoluble in water, but dissolve easily with the aid of a little alkali. Such a solution, neutral or, indeed, a faintly acid one, does not coagulate on boiling. The nucleoalbumins resemble the globulins and the albuminates in solubility and precipitation properties, but differ from them in being hardly soluble in neutral salts. The most important difference between the nucleoalbumins, the globulins, and the albu- minates is that the nucleoalbumins contain phosphorus, and by the action of pepsin hydrochloric acid on nucleoalbumins a phosphor- ized product, nuclein, is split off which, according to LIEBER- MANN, is a combination of albumin with metaphosphoric acid. The nucleoalbumins seem habitually to contain less sulphur than the bodies of the preceding groups. Some iron is found as a con- stant constituent. Alkali and Acid Albuminates. By the action of alkalies all native albuminous bodies are converted, with the elimination of nitrogen or by the action of stronger alkali with the emission of sulphur, into a new modification, called alkali albuminate, whose specific rotation is increased at the same time. If caustic alkali in substance or in strong solution be allowed to act on a concentrated albumin solution, such as blood-serum or egg-albumin, the alkali albuminate may be obtained as a solid which dissolves in water on heating forming a jelly, and which is called " LIEBERKUHN'S solid alkali albuminate." By the action of dilute caustic alkali solutions on dilute albumin solutions we have alkali albuminates, formed slowly at the ordinary temperature, but more rapidly on heating. These solutions may be modified by the source of the albumin acted upon, and also by the extent of the action of the alkali, but it is still always the same reaction. If albumin is dissolved in an excess of concentrated hydro- chloric acid, or if we digest an albumin solution acidified with 1-2 p. m. hydrochloric acid in the warmth, or digest the albumin alone with pepsin hydrochloric acid, we obtain a new modification of albumin which indeed may show somewhat varying properties, but has also certain reactions in common with ordinary albumin. 24 PHYSIOLOGICAL CHEMISTRY. These modifications, which may be obtained in a solid gelatinous condition by sufficient concentration, are called acid albuminates or acid albumin, sometimes also syntonin, though we prefer to call that acid albuminate syntonin which is obtained by extracting muscles with hydrochloric acid of 1 p. m. The alkali and acid albuminates have the following reactions in common : They are nearly insoluble in water and dilute common- salt solutions (see previous page), but they dissolve easily in water after the addition of a very small quantity of acid or alkali. Such a solution or one nearly neutral does not coagulate on boiling, but is precipitated at the normal temperature on neutralizing the sol- vent by an alkali or an acid. A solution of an alkali or acid albu- minate in acid is easily precipitated on saturating with NaCl, but a solution in alkali is precipitated with difficulty or not at all, according to the amount of alkali it contains. The nearly neutral solutions are precipitated by mineral acids in excess, also by many metallic salts. Notwithstanding this agreement in the reactions, the acid and alkali albuminates are essentially different, and by dissolving an alkali albuminate in some acid no acid albuminate solution is obtained, nor is an alkali albuminate formed on dissolving an acid albuminate in water by the aid of a little alkali. The alkali albuminates are relatively strong acids. They may be dissolved in water with the addition of CaC03, with the elimination of C0a, which does not occur with typical acid albuminates, and they show in opposition to the acid albuminates also other variations which stand in connection with their strongly-marked acid nature. Dilute solutions of alkalies act more energetically on albumin than do acids of the same concentration. In the first case a part of the nitrogen, and often also the sulphur, is split off, and from this property we may obtain an alkali albuminate by the action of an alkali upon an acid albuminate; but we cannot obtain an acid albuminate by the reverse reaction. (K. MOEKEK.) The preparation of the albuminates has been given above. By the action of alkalies or acids upon an albumin solution the corre- sponding albuminate may be precipitated by neutralizing with acid or alkali. The washed precipitate is dissolved in water by the aid of a little alkali or acid, and again precipitated by neutralizing the THE PROTEIN SUBSTANCES. 25 solvent. If this precipitate which has been washed in water is treated with alcohol and ether, the albumiuate will be obtained in a pure form. Albumoses and Peptones. Peptones are designated as the final products of the decomposition of albuminous bodies by means of proteolytic enzymes, in so far as these final products are still true albuminous bodies; while we designate as albumoses or propeptones the intermediate products produced in the peptonization of albu- mins in so far as they are substances not similar to albuminates. Albumoses and peptones may also be produced by the hydro- lytic decomposition of the albumins with acids or alkalies, also by the putrefaction of the same. They may also be formed in very small quantities as by-products in the investigations of animal fluids and tissues, and the question to what extent these exist pre- formed under physiological conditions requires very careful inves- tigation. Between the peptones which represent the last splitting prod- ucts and those albumoses which stand closest to the original albumin we have undoubtedly a series of intermediate products. Under such circumstances it is a difficult problem to try to draw a sharp line between the peptone and the albutnose group, and it is just as difficult to define our conception of peptones and albumoses in an exact and satisfactory manner. The albumoses have been considered as those albuminous bodies whose solutions do not coagulate on boiling and which, to dis- tinguish them from peptones, were characterized chiefly by the following properties. The watery solutions are precipitated at the ordinary temperature by nitric acid as well as by acetic acid and potassium ferrocyanide, and this precipitate has the peculiarity of disappearing on heating and reappearing on cooling. If a solution of albumoses is saturated with NaCl in substance, the albumoses are partly precipitated in neutral solutions, but on the addition of acid saturated with the salt they completely precipitate.- This pre- cipitate, which dissolves on warming, is a combination of albumose with the acid. We formerly designated as peptone that albuminous body which was readily soluble in water and which did not coagulate by heat, whose solutions were precipitated neither by nitric acid, nor by 26 PHYSIOLOGICAL CHEMISTRY. acetic acid and potassium ferrocyanide, nor by neutral salts and acid. The reactions and properties which the albumoses and peptones had in common were formerly considered as the following: They give all the color reactions of the albumins, but with the biuret test they give a more beautiful red color than the ordinary albumin. They are precipitated by ammoniacal lead acetate, by mercuric chloride, alcohol, tannic, phospho-tungstic, phospho-molybdic acids, potassium-mercuric iodide and hydrochloric acid, and lastly by picric acid. The albumoses and peptones have also a greater diffusive power than native albuminous bodies, and the diffusive power is greater the nearer the questionable substance stands to the final product, the so-called pure peptone. These old views have undergone an essential change in the last few years. After HEYNSIUS' observation that ammonium sulphate was a general precipitant for albumin, also peptone in the old sense, KUHKE and his pupils proposed this salt as a means of sepa- rating albumoses and peptones. Those products of digestion which separate on saturating their solution with ammonium sulphate are considered by KUHNE and indeed by most of the modern investi- gators as albumoses, while those which remain in solution are called peptones or pure peptone. This pure peptone is formed in rela- tively large amounts in the pancreatic digestion, while in the pepsin digestion it is only small in quantity unless after prolonged diges- tion. According to SCHUTZEN BERGER and KUH^E the albumins yield two chief groups of new albuminous bodies when decomposed by dilute acids or with proteolytic enzymes; of these the anti group shows a greater resistance to further action of the acid and enzyme than the other, namely, the hemi group. Corresponding to these views KUHNE divides the albumoses into two chief groups, the antialburnoses and liemialbumoses, and the peptones into two chief groups, the' antipeptoms and the liemipeptones. In the pepsin digestion we obtain, besides different albumoses, a mixture of anti- and hemipeptone, which mixture KUHNE called ampliopeptone. In the digestion with trypsin (the proteolytic enzyme of the pan- creas) the hemipeptone is further split into leucin, tyrosin, and other substances, while the antipeptone remains unchanged. Only THE PROTEIN SUBSTANCES. 27 by the sufficiently energetic action of trypsin is one peptone at last obtained, the so-called antipeptone. KUHNE and his pupils, who have conducted these complete investigations on the albumoses and peptones, classify the different kinds of albumoses according to their different solubilities and pre- cipitation powers. In the pepsin digestion of fibrin they obtained the following albumoses: 1. Dysalbumose, which is insoluble in water and dilute salt solutions. 2. Heteroalbumose, insoluble in water but soluble in salt solution. 3. Protalbumose, soluble in salt solution and water. These three albumoses are precipitated by NaCl in a neutral solution, while 4, the D enter oalbumose, which is soluble in salt solution or water, is precipitated (partly) on saturat- ing with NaCl, but only after the addition of an acid. This pre- cipitate is a combination of albumose and acid (HEETH). HERTH claims that the relative proportion of acid or alkaki, salt, water, or albumose in a solution essentially changes the solu- bility and precipitation power of the same. He also claims that the occurrence of several different kinds of albumoses cannot be demonstrated, because with one and the same albumose, the above conditions being changed, its solubilities and precipitating powers are changed. HAMBUEGER found the same to be true from his investigations. The albumoses obtained from different albuminous bodies do not seem to be identical. The globulinalbumoses of KUHNE and CHITTENDEN are called globuloses, the vitellin albumoses of NEUMEISTER vitelloses, those of casein caseoses (CHITTENDEN), those of myosin myosinoses (KUHNE and CHITTENDEN), and so on. The different kinds of albumoses are distinguished as proto,- hetero-, and deutero-aiseoses, etc. NEUMEISTER designates as atmidalbumose that body which is obtained by the action of superheated steam on fibrin. At the same time he also obtained a substance called atmidalbumin which stands between the albuminates and the albumoses. Of the soluble albumoses NEUMEISTER designates protoalbumose and heteroalbumose as primary albumoses, while the deuteroalbu- mose, which is nearly related to the peptones, he calls secondary albumose. As essential difference between the primary and sec- ondary albumoses he suggests the following: The secondary albu- moses are not precipitated by nitric acid in liquids free from salt, nor by copper sulphate solution (2 parts in 100), nor by NaCl in substance in a neutral liquid. Pure true peptones are not precipi • 28 PHYSIOLOGICAL CHEMISTRY. tated either by picric acid or by potassium-mercuric iodide and acid. The primary albumoses are completely precipitated by phospho-molybdic or phospho-tungstic acid, while the secondary are not quite completely precipitated, and the true peptones very incompletely. The peptones are also precipitated by mercuric chloride in neutral solutions, and also by tannic acid in liquids con- taining acetic acid. This precipitate may be dissolved in an excess of tannic acid (SEBELIEN"). The study of the albumoses and peptones, as above indicated, has undergone in the last few years an essential transformation. It may still be doubtful whether the behavior of a single salt, the ammonium sulphate, yields sufficient basis for the characterization of two groups of albuminous bodies, the albumoses and peptones ; and this question is warranted since, according to NEUMEISTEE, we have a deuteroalbumose (found in the pepsin digestion) which is not completely precipitated by ammonium sulphate. It seems that the transformation of albumins into peptones takes place through a number of intermediate steps such as starch undergoes in passing from dextrine into glucose. A complete separation of these several intermediate products, as well as their purification, is an extremely difficult task. What relationship do the albumoses and peptones bear to the albumin from which they are formed ? HEBTH has found that fibrin albumose and fibrin have a similar constitution. KUHKE and CHITTTENDEIJ, as also CHITTE^DEN" and his pupils, have ana- lyzed the different albumoses from fibrin, globulin, egg-albumin, myosin, and casein, and found in a few albumoses an increase and in others a decrease in the amount of carbon, nitrogen, and sulphur as compared with the mother-albumin. From the results of their analyses it has been found that, with the probable excep- tion of the peptone standing closest to the albumoses, the difference in the constitution of the original albumins and the corresponding albumose is sometimes in one direction and sometimes in another, and is unessential. According to the analyses of peptones (in the old sense) made by MALT, HERTH, and HEKNINGEB, they seem to have the same constitution as the albumin. According to the analyses by KUHNE and CHITTE^DEK of "true" fibrin peptone, part amphopeptone THE PROTEIN SUBSTANCES. 29 and part antipeptone prepared by pancreas infusion, this peptone was found to contain about the same amount of hydrogen and the same or a greater amount of nitrogen, but less carbon than the albu- moses. In his investigations on casein CHITTEKDEN found, on the other hand, that in antipeptone the amount of carbon was higher than in certain caseoses. As the preparation of true peptones in a pure condition is accompanied with great difficulty, and as the peptones (in the modern sense) analyzed have not always behaved as true peptones towards the peptone reagents as described by NEUMEISTER, it is most difficult to draw any positive conclusion from these analyses. It seems, nevertheless, that generally the so-called true peptones are perhaps somewhat poorer in carbon than the corresponding albumins. The elementary analyses made up to the present time have not given us a positive answer in regard to the relationship existing between the albumins on one side 'and the albumoses and peptones on the other. The view that the peptone formation is a hydrolytic splitting is accepted by HOPPE-SEYLER, KUHKE, HENNINGER, and indeed by recent investigators. In support of this view we have the observations of HENNINGER and HOFMEISTEK, according to which peptones are converted into an albumin similar to albu- minates by the action of acetic acid anhydride, or by heating so that water is expelled. According to other investigators, as MALT, HERTH, LOEW, and others, the formation of peptone is a depoly- merization of the albumins. A third view is that albumins and peptones are isomeric bodies; while a fourth view (GRIESSMAYER) claims that the albumins consist of micell groups which on pepto- nization are first converted into micells and then further into a molecule. Though an ordinary albumin solution contains micells or micell bonds, so also a peptone solution contains an albumin molecule. The preparation of different albumoses in a complete pure form is very troublesome and accompanied with a great many difficulties. For this reason there will be given here only the general methods by which the different albumose precipitates are obtained. If we pro- ceed from a solution of fibrin in pepsin hydrochloric acid, we first neutralize, heat to boiling, filter, concentrate the filtrate, and satu- rate it with common salt in substance. The precipitate is filtered, 30 PHYSIOLOGICAL CHEMISTRY. washed with a saturated salt solution, and then treated with a 10 per cent NaCl solution; what remains is called dysalbumose. The filtered solution is repeatedly and completely dialyzed. A part separates which is heteroalbumin, while the protalbumose remains dissolved in the liquid. The above-mentioned filtrate which has had the primary albumoses removed and saturated with common salt is treated with acetic acid which has previously been saturated with salt. The precipitate consists of deuteroalbumose. The re- sulting filtrate, from which a new precipitate separates on satura- tion with ammonium sulphate, contains the remainder of the deuteroalbumose and a little peptone. For the preparation of true peptone we may use a prolonged pepsin digestion, but much quicker results are obtained by the use of the trypsin digestion. The neutralized liquid is heated to boiling, filtered, sufficiently concentrated, and saturated while boiling hot with ammonium sulphate. The albumoses which sepa- rate are filtered. If any peptone is present in the cold filtrate, a beautiful biuret reaction is obtained by the addition of very strong caustic soda solution and a little' copper sulphate solution, though when the products of pepsin digestion are employed this may depend on non-precipitated deuteroalbumose. The greater part of the ammonium sulphate may be removed from the filtrate by evap- oration and crystallization or by partial precipitation by alcohol. What now remains is separated by the addition of barium hydrate, and lastly by barium carbonate with heat. The filtrate is concen- trated and precipitated by alcohol. In regard to the more detailed accounts of the proposed methods for the preparation of pure pep- tone and the different albumoses the reader is referred to the works of KUHNE, CHTTTENDEN, and NEUMEISTEK. The methods for the detection of albumoses and peptones in fluids and tissue are not very accurate, and therefore, as they can- not be given without criticism which does not enter into the scope and plan of this work, they will be omitted. Coagulated Albuminous Bodies. Albumin may be converted into the coagulated condition by different means : by heating (see page 18), by the action of alcohol, especially in the presence of neutral salts, and in certain cases, as in the conversion of fibrinogen into fibrin (Chapter IV), by the action of an enzyme. The nature of the processes which take place during coagulation is unknown. The coagulated albuminous bodies are insoluble in water, in neutral salt solutions, and in dilute acids or alkalies, at normal temperature, They are dissolved and converted into albuminates by the action of less dilute acids or alkalies, especially on heating. THE PHOTEIN SUBSTANCES. 31 II. Proteid. With this name, as suggested by HOPPE-SEYLER, we designate a class of bodies which are more complex than the albuminous bodies and which yield as nearest splitting products albuminous bodies on one side and non-protein bodies, such as coloring matters and carbohydrates, on the other. The most important substances belonging to this group are the blood - coloring matter, haemoglobin, which will be thoroughly treated in a following chapter (Chapter IV), and mucin substances or allied bodies. Mucin Substances. We designate as mucin colloid substances whose solutions are mucilaginous and thready, and which when treated with acetic acid give a precipitate insoluble in an excess of acid, and on boiling with dilute mineral acids yield a substance capable of reducing copper oxyhydrate. This last-mentioned fact, which was first observed by EICHWALD, differentiates mucin from other bodies which have long been mistaken for it and .which have similar physical properties. On the other hand, bodies whose phy- sical properties differ from it but which give a reducible substance on boiling with dilute mineral acids have also been designated as mucin. The different bodies characterized as mucin substances cor- respond, first, either to true mucin or, second, to mucoid or mucinoid. All mucin substances contain carbon, hydrogen, nitrogen, sulphur, and oxygen. Compared with albuminous bodies they contain less nitrogen and, as a rale, less carbon. As close decom- position products they yield albuminous bodies on one side and carbohydrates or acids related thereto on the other. On boiling with dilute mineral acids they all give a reducible substance. The true mucins are characterized by their natural solution, or one prepared by a trace of alkali, being mucilaginous, thread-like, and giving a precipitate with acetic acid which is insoluble in excess of acid. The mucoids do not show these physical properties and have other solubilities and precipitative properties. As we have intermediate steps between different albuminous bodies, so also we have such between true mucins and mucoids, and a sharp line between these two groups cannot be drawn. 32 PHYSIOLOGICAL CHEMISTRY. True mucins are secreted by the larger mucous glands, by certain mucous membranes, also by the skin of snails and other animals. True mucin also occurs in the connective tissue and navel-cord. Some- times, as in snails and in the membrane of the frog-egg (GiACOSA), a mother - substance of mucin, a mucinogen, has been found, which may be converted into mucin by alkalies. Mucoid substances are found in cartilage, certain cysts, etc. As the mucin question has been very little studied, it is at the present tiij.e impossible to give any positive statements in regard to the occurrence of mucins and mucoids, especially as without doubt in many cases non-mucinous substances have been described as mucins. So much is sure, that mucins or nearly-related bodies occur widely diffused in the organism of certain tissues. From their decomposition prod- ucts we derive a great deal of knowledge in regard to the forma- tion and splitting of carbohydrates or kindred bodies (glycuronic acid) from other complex atoms. True Mucin. Thus far we have been able to obtain only a few mucins in a pure and unchanged condition due to the reagents used. The elementary analyses of these mucins have given the following results : C H N S O Mucin from snail 5032 6.84 13.65 1.75 27.44 (HAMMARSTEN.) Mucin from nerve 48.30 6.44 11.75 0.81 32.70 (LOEBISCH.) Mucin from sub-maxillaris 48.84 6.80 12.32 0.84 31.20 (HAMMARSTEN.) The mucin of the snail-skin, which stands closest to keratin, contains more sulphur than the other mucins. The sulphur is moreover, at least in certain mucins, part in loose and part in strong chemical union. By the action of superheated steam on mucin a carbohydrate, animal gum (LANDWEHR), is split off. On boiling mucin with dilute mineral acids, acid albuminate and bodies similar to albu- mose or peptone are obtained, besides a reducing substance which has not been closely studied. By the action of stronger acids we obtain among other bodies leucin, tyrosin, and laevulinic acid (LAISTDWEHR). Certain mucins, as the submaxillaris mucin, are easily changed by very dilute alkalies, as lime-water, while others, such as nerve-mucin, are not affected (LOEBISCH). If a strong THE PROTEIN SUBSTANCES. 33 caustic alkali solution, as a 5 per cent KOH solution, is allowed to act on sub-maxillaris mucin, we obtain alkali albuminate, a body similar to albumose and peptone, and one or more substances of an acid reaction and with strong reducing powers. In one or the other respect the different mucins act somewhat differently. For example, the snail and nerve mucins are insoluble in dilute hydrochloric acid of 1-2 p. m., while the mucin of the submaxillary gland and the navel cord are soluble. Nerve-mucin becomes flaky with acetic acid, while the other mucins are precipi- tated in more or less fibrous, tough masses. Still all the mucins have certain reactions in common. In the dry state mucin forms a white or yellowish-gray powder. When moist it forms, on the contrary, flakes or yellowish-white tough lumps or masses. The mucins are acid in reaction. They give the color reactions of the albuminous bodies. They are not soluble in water, but may give a neutral solution with water and the smallest quantity of alkali. Such a solution does not coagulate on boiling, while acetic acid gives at the normal temperature a precipitate which is insoluble in an excess of the precipitant. If 5-10$ NaCl be added to a mucin solution, this can now be care- fully acidified with acetic acid without giving a precipitate. Such acidified solutions are copiously precipitated by tannic acid; with potassium ferrocyanide they give no precipitate, but on sufficient concentration they become thick or viscous. A neutral solution of mucinalkali is precipitated by alcohol in the presence of neutral salts; they also give precipitates with several metallic salts. If mucin is heated on the water-bath with dilute hydrochloric acid of about 2$, the liquid gradually becomes a yellowish or dark brown and reduces copper oxhydrate from alkaline solutions. The mucin most easily obtained in large quantities is the sub- maxillary mucin, which may be prepared in the following way: The filtered watery extract of the gland, as colorless as possible, is treated with 25$ hydrochloric acid, so that the liquid contains 1.5 p. m. HC1. On the addition of the acid the mucin is imme- diately precipitated, but dissolves on stirring. If this acid liquid is immediately diluted with 2-3 vols. of water, the mucin separates and may be purified by redissolving in 1-5 p. m. acid and diluting with water and washing therewith. The mucin of the navel-cord 34 PHYSIOLOGICAL CHEMISTRY. may be prepared in the same way.1 The nerve mucin Is prepared from nerves which have first been freed from albumin by common- salt solution and water. They are extracted with lime-water, the filtrate is precipitated with acetic acid, and the precipitate purified by redissolving in dilute alkali or lime-water, precipitating with acid, and washing with water (ROLLETT, LOEBISCH). Lastly, the mucin is treated with alcohol. 2. Mucoids or Mucinoids. To this group belong pseudomucin, which occurs in ovarial liquids, colloid, which is probably related thereto, and chondromucoid, which occurs in cartilage. These bodies will be treated of later in their respective chapters. Hyalogen. Under this name KRUKENBEBG has designated a number of differing protein bodies, which are characterized by the following: By the action of alkalies they change, with the splitting off of sulphur and nitrogen, into a soluble uitrogeuized product called by him hyaline and which yields a pure carbohydrate by further decomposition. Within this group the most widely differing substances may find place, as for instance true mucin and mucoid, the so-called mucin of the holothuria, neossin of the edible bird'snest, the glycoproteid of the vineyard snail, the onuphin and spirographin, and other substances from the lower animals. It is of very little value to collect into one group all these differing substances, which have very little in common, until we have learned with some degree of certainty the nature of the re- ducible substances and other products obtained from them. III. Albumoids or Albuminoids. iJnder this name we collect into a special group all those pro- tein bodies which cannot be placed in either of the other two groups, although they differ essentially among themselves and from a chemical standpoint do not show any prevailing difference from the ordinary albuminous bodies. The most important and abun- dant of the bodies belonging to this group are important con- stituents of the animal skeleton or the animal structure. They occur as a rule in an insoluble state in the organism, and they are marked in most cases by a great resistance to reagents which dis- solve albumins or to chemical reagents in general. The Keratin Group. Keratin is the chief constituent of the horny structure, of the epidermis, of hair, wool, of the nails, hoofs, horns, feathers, of tortoise-shell, etc., etc. Keratin is also found as neurokeratin (KiJHKE) in the brain and nerves. The shell-mem- brane of the hen's egg seems also to contain keratin. 1 The author has not been able to obtain this pure, so the analysis has not been given in the previous table of the mucins. THE PROTEIN SUBSTANCES. 35 It seems that there exist more than one keratin, and these varieties form a special group of bodies. This fact, together with the difficulty in isolating the keratin from the tissues in a pure condition without a partial decomposition, is sufficient explanation for the variation in the elementary constitution given below. As examples the analyses of a few tissues rich in keratin and of kera- tin itself are given as follows : c H N so Human hair .... 50.65 6.36 17.14 5.00 20.85 (v. LAAB.) Nail 51.00 6.94 17.51 2.80 21.75 (MULDER.) Neurokeratin... 56.11-58.45 7.26-8.02 11.46-14.32 1.63-2.24 .... (KUHNE.) Horn (average). 50.86 6.94 .... 3.30 .... (HORBACZEWSKI.) Tortoise-shell... 54.89 6.56 16.77 2.22 19.56 (MULDER.) Shell-membrane 49.78 6.64 16.43 4.25 20.90 (LINDVALL.) Sulphur is at least in part in loose combination, and it is partly removed by the action of alkalies (as sulphides), or indeed by boil- ing with water (CHEVREUL). Combs of lead after long usage become black, and this is due to the action of the sulphur of the hair. On heating keratin with water in sealed tubes at a tempera- ture of 150° to 200° C. it dissolves, with the elimination of sulphu- retted hydrogen, forming a non-gelatinizing liquid which contains albumose (called keratinose by KRUKENBERG) and peptone (?). The keratin may be dissolved by alkalies, especially on heating, forming, besides alkali sulphides, albumoses and peptones (?). That the keratin in the organism is formed from the albumin is not to be denied. DRECHSEL believes that in keratin a part of the oxygen of the albumins is exchanged for sulphur, and a part of the xeucin or any other amido-acid is exchanged for tyrosin. The products of decomposition of keratin and of albumin are similar, except that the former gives proportionally a greater amount of tyrosin (3-5$). Keratin is amorphous or takes the form of the tissues from which it was prepared. On heating it decomposes and generates an odor of burnt horn. It is insoluble in water, alcohol, or ether. On heating with water to 150°-200° C. it dissolves. It also dissolves gradually in caustic alkalies, especially on heating. It is not dissolved by artificial gastric juice or by trypsin solutions. Keratin gives the xanthoproteic acid reaction, as well as the reac- 36 PHYSIOLOGICAL CHEMISTRY. tions with MILLON'S reagent, even though they are not always, typical. In the preparation of keratin a finely-divided horny structure is treated first with boiling water, then consecutively with diluted acid, pepsin-hydrochloric acid, and alkaline trypsin solution, and, lastly, with water, alcohol, and ether. Elastin occurs in the connective tissue of higher animals, pome- times in so large quantities that it forms a special tissue. It occurs most abundantly in the cervical ligament (ligamentum nuchae). It seems that there is more than one kind of elastin. Elastin, according to the general view, is free from sulphur. According to the investigations of CHITTENDEK and HAKT, it is a question whether or not elastin contains sulphur which is removed by the action of the alkali in its preparation. The most trust- worthy analyses of elastin from the cervical ligament have given the following results : C H >T O 54.32 6.99 16.75 21.94 (HORBACZEWSKI.) 54.24 7.27 16.70 21.79 (CHITTBNDEN and HART.) As splitting products we find leucin, tyrosin (in small quantity), glycocoll, amido-valerianic acid, ammonia, and others. No indol or phenol is obtained on putrefaction. On heating with water in closed vessels, on boiling with dilute acids, or by the action of a proteolytic enzym, the elastin dissolves and splits into two chief products, caKed by HOEBACZEWSKI Jiemielastin and elastinpeptone. According to CHITTEKDEN and HAKT, these products correspond to two albumoses designated by them protoelastose and deutero- elastose. The first is soluble in cold water and separates on heating, and its solution is precipitated by mineral acid as well as by acetic acid and potassium ferrocyanide. The watery solution of the other does not become cloudy on heating, and is not precipitated by the above-mentioned reagents. Pure dry elastin is a yellowish-white powder; in the moist state it appears like yellowish-white threads or membranes. It is insol- uble in water, alcohol, or ether, and shows a resistance against the action of chemical reagents. It is not dissolved by strong caustic THE PROTEIN SUBSTANCES. 37 alkalies at the ordinary temperature, and only slowly at the boiling temperature. It is very slowly attacked by cold concentrated sul- phuric acid, and it is relatively easily dissolved on warming with strong nitric acid. It gives MILLON'S reaction. On account of its great resistance to chemical reagents, elastin may be prepared (best from the ligameiitum nuchae) in the fol- lowing way : First boil with water, then with \% caustic potash, then again with water, and lastly with acetic acid. The residue is treated with cold 5$ hydrochloric acid for twenty-four hours, carefully washed with water, boiled again with water, and then treated with alcohol and ether. Collagen, or glue-forming substance, occurs very extensively in the animal kingdom, especially in the vertebrates, seldom in the invertebrates. Collagen is the chief constituent of the fibres of the connective tissue and (as ossein) of the organic substances of the bony structure. It also occurs in the cartilaginous tissues as chief constituent, but it is here mixed with another substance which was formerly called chondrigen. Collagen from different tissues has not quite the same composition, and probably there are several varieties of collagen. By continuously boiling with water (more easily in the presence of a little acid) collagen is converted into gelatine. HOFMEISTER found that gelatine, on being heated to 130° C., is transformed into collagen; and this last may be considered as the anhydride of gela- tine. Collagen and gelatine have the following composition : C H N S+0 Collagen 50.75 6.47 17.86 24.92 (HOFMEISTER.) Gelatine (from hartshorn) . 49.31 6.55 18.37 25.77 (MULDER.) Gelatine (from bones) 50.00 6.50 17.50 26.00 (FREMY.) The gelatine contains about 0.6$ sulphur, which probably be- longs to the gelatine and hardly exists there as an impurity from the albumin. The investigations in regard to the decomposition products of collagen have been made on gelatine. Gelatine yields, under simi- lar conditions as albuminous bodies, amido-acids, but no tyrosin. It yields a large amount of glycocoll, to which, on this account, the name of glue-sugar has been given. On putrefaction gelatine gives 38 PHYSIOLOGICAL CHEMISTRY. neither tyrosin nor indol, in which it deviates from e albumins. Still the aromatic group is not absent in gelatine, and it acts like the oxidized albumin, the oxyprotsulphonic acid giving benzoic acid (MALY). On treating gelatine with hydrochloric acid and alcohol and then acting on this with a nitrite, BUCHNER and CUR- TIUS obtained an ester of a diazo-fatty acid, probably diazo-oxyacry- lic acid ester, and it is therefore also possible that the nucleus of the gelatine is formed of amido-acrolein. Collagen is insoluble in water, salt solutions, dilute acids, and ulkalies, but it swells up in dilute acids. By continuous boiling with water it is converted into gelatine. It is dissolved by the gastric juice and also by the pancreatic juice (trypsin solution) when it has previously been treated with acid or heated with water above -f- 70° C. By the action of ferrous sulphate, corrosive sublimate, or tannic acid, collagen shrinks. Collagen treated by these bodies does not putrefy, and the tannic acid is therefore of great importance in the preparation of leather. Gelatine or glue is colorless, amorphous, and transparent in thin layers. It swells in cold water without dissolving. It dissolves in warm water, forming a sticky liquid, which solidifies on cooling when sufficiently concentrated. The solution is laevogyrate; a] at -j- 30° C. = — 130°. Acetic acid and alkalies diminish the specific rotary power. Gelatine solutions on boiling are not precipitated either by mineral acids, acetic acid, alum, lead acetate, or mineral salts in general. A gelatine solution acidified with acetic acid may be precipitated by potassium ferrocyanide on carefully adding the reagent, but on the addition of too much potassium ferrocyanide the liquid remains clear. Gelatine solutions are precipitated by tannic acid in the presence of salt; by acetic acid and common salt in substance ; mercuric chloride in the presence of HC1 and NaCl ; phosphomolybdic acid in the presence of acid ; and lastly by alcohol, especially when neutral salts are present. Gelatine solu- tions do not diffuse. Gelatine gives the biuret reaction, but not ADAMKIEWICZ'S. It gives MILLON'S reaction and the xanthoproteic acid reaction so faintly that it probably occurs from an impurity consisting of albumin. By continuous boiling with water, — espe- cially in the presence of dilute acid, — also by digesting with gastric THE PROTEIN SUBSTANCES. 39 juice or trypsin solution, gelatine loses the property of gelatin- izing and is transformed into gelatine-peptone. According to HOFMEISTER it splits into two substances, semiglutin and hemicollin. The former is insoluble iu alcohol of 70-80$, and is precipitated by platinum chloride. The latter, which is not precipitated by platinum chloride, dissolves in alcohol. Collagen may be obtained from bones by extracting with hydro- chloric acid (which dissolves the earthy matters) and then carefully removing the acid with water. It may be obtained from tendons treated with lime-water (which dissolves the albumin and mucin), and then thoroughly washing with water. Gelatine is obtained by boiling collagen with water. The finest commercial gelatine con- tains a little albumin, which may be removed by allowing the finely- divided gelatine to swell in cold water and extracting thoroughly with large quantities of fresh water. In regard to the preparation of gelatine from cartilage see Chapter VIII. Chondrin is only a mixture of glue with the specific constituents of cartilage and their transformation products. Spongin forms the great mass of the ordi- nary sponge. It gives no gelatine, and on boiling with acids it yields leucin and glycocoll, but no tyrosiu. Conchiolin is found in the shells of mussels and snails, and also in the egg-shells of these animals. It yields leuciu but no tyrosin. Byssus contains a substance, closely related to conchiolin, which is soluble with difficulty. Cornein forms the axial system of the Antipathes and Gorgonia. It gives leucin and a crystallizable substance, cornicrystallin (KRU- KENBERG). Fibroin and Sericin are the two chief constituents of raw silk. By the action of superheated water the sericin dissolves and gelatinizes on cooling (silk gelatine), while the more difficultly soluble fibroin remains un- dissolved in the shape of the original fibre. On boiling with acid the fibroin yields alanin (WEYI,), glycocoll, and a great deal (5-8$) of tyrosin. Fibroin is dissolved in cold concentrated hydrochloric acid with the expulsion of \% ni- trogen as ammonia, and it is converted into another, nearly-related substance called sericoin (WfiYL). Sericin yields no glycocoll but leucin and a crystal- lizable substance called serin. The composition of the above-mentioned bodies is as follows : C H N S O Conchiolin (from snail-eggs) 50.92 6.88 17.86 0.31 24.34 (KRUKENBERG ) Spongin 46.50 6.30 16.20 0.5 27.50 (CROOCKEWITT.) 48.75 6.35 16.40 (POSSELT.) Cornein 48.96 5.90 1681 .... 28.33 (KRUKENBERG.) Fibroin 48.23 6.27 18.31 .... 27.19 (CRAMER.) Sericin 44.32 6.18 18.30 .... 80.20 (CRAMER.) Amyloid, so called by VIRCHOW, is a protein substance appear- ing under pathological conditions in the internal organs, such as the spleen, liver, and kidneys, as infiltrations ; and in serous mem- branes as granules with concentric layers. It probably also occurs as a constituent of a few prostate calculi. Amyloid has not been 40 PHYSIOLOGICAL CHEMISTRY. obtained pure, therefore its composition cannot be given with cer- tainty. FKIEDREICH and KEKULE found C 53.6; H 7.0; N 15.0; and S + 0 24.4$. KUHNE and RUDNEFF found 1.3$ sulphur. Amyloid is not related to the carbohydrates, and on boiling with acids it gives neither glucose nor any other reducing substance. On the contrary, *it yields leucin and tyrosin. It is insoluble in water, alcohol, ether, dilute hydrochloric acid, and acetic acid. It is dissolved in concentrated hydrochloric acid or caustic alkali, and is converted into acid or alkali albuminates according to the agents employed. According to KOSTJUKIN, amyloid is dissolved by the gastric juice, which is the reverse of older theories. Amyloid gives the xanthoproteic , acid reaction and the reactions of MILLON and ADAMKIEWICZ. Its most important property is its behavior with certain coloring matters. It is colored reddish brown or a dingy violet by iodine; a violet or blue by iodine and sulphuric acid ; red by methylaniline iodide, especially on the addition of acetic acid ; and red by aniline green. Amyloid is prepared by extracting the tissue with cold and then boiling water, afterwards with alcohol and ether. After boil- ing with alcohol containing hydrochloric acid and digesting with gastric juice, that which is insoluble is considered as amyloid. As the amyloid may be dissolved by the gastric juice, the utility of this method seems doubtful. CHAPTER III. THE ANIMAL CELL. THE cell is the unit of the manifold, variable forms of the organ- ism ; it forms the simplest physiological apparatus, and as such is the seat of chemical processes. It is generally admitted that all chemi- cal processes of importance do not take place in the animal fluids, but proceed in the cells, which may be considered as the chemical laboratory of the organism. It is also principally the cells which, through their greater or less activity, regulate or govern the range of the chemical processes and also the intensity of the total ex- change of material. It is natural that the chemical investigation of the animal cell should in most cases coincide with the study of those tissues of which it forms the chief constituent. Only in a few cases can the cells be directly, by relatively simple manipulations, isolated in a rather pure state from the tissues, as, for example, in the investiga- tion of pus or of tissue very rich in cells. But even in these cases the chemical investigation may not lead to any positive results in regard to the constituents of the uninjured living cells. By the process of chemical transformation new substances may be formed at the death of the cell, and at the same time physiological constitu- ents of the cell may be destroyed or transported into the surrounding menstruum and therefore escape investigation. For this and other reasons we possess only a very limited knowledge of the constituents and the constitution of the cell, especially of the living one. While young cells of different origin in the early period of their existence may show a certain similarity in regard to their form and chemical constitution, they may, on further development, not only take the most varied forms, but may also offer from a chemical standpoint the greatest diversity. As a description of the con- stituents and the constitution of the different cells occurring in the animal organism is nearly equivalent to a demonstration of the chemical relations of most animal tissues, and as this exposition 41 42 PHYSIOLOGICAL CHEMISTRY. will be found in their respective chapters, we will here only discuss the chemical constituents of the young cells or the cells in general. We must first differentiate between the protoplasm and the nucleus. The Protoplasm of the • generative cell consists during life of a semi-solid body contractile under certain conditions, very rich in water, and the mass of which consists mainly of albuminous bodies. If the cell be deprived of the physiological conditions of life, or if exposed to destructive exterior influences, such as the action of high temperatures, of chemical agents, or indeed of distilled water, the protoplasm dies. The albuminous bodies which it contains co- agulate at least partially, and other chemical changes are found to take place. The alkaline reaction of the living cell may be converted into an acid by the appearance of paralactic acid, and the carbohy- drate, the glycogen, which habitually occurs in the young genera- tive cell may after its death be quickly changed and consumed. The albuminous bodies of the protoplasm consist, according to the general view, chiefly of globulins, but albumins are also found. The occurrence of globulins in the animal as well as in the vegetable cell has been specially shown by HOPPE-SEYLER, and according to this investigator two globulin substances, vitellin and myosin, occur in all protoplasm. HALLIBURTON has lately closely studied the albuminous bodies of the lymphatic cells, and found two globulins besides an albumin probably identical with serum albu- min (see Chapter IV). Of the globulins, one which occurs only in small quantities coagulates at the temperature of 48-50° C., while the other, which occurs in abundance, is coagulated like serum globulin by a 5$ solution of MgS04 at 75° C. The widespread occurrence of globulins and also of albumins in the protoplasm of the animal cell has been unquestionably demonstrated, but these two groups of albuminous bodies do not, at least in many cases, form the chief mass of the protoplasm. The protoplasm seems to consist in great part of very complex protein substances, the proteids on one side and the nucleoalbumins on the other. Of all these the nucleoalbumins appear to be regular constituents of the protoplasm, and they do not only appear in the pus cells or in the cells of the lymphatic glands (HALLIBURTON), but also in almost all varieties of glandular cells. The chief mass of the albumins found THE ANIMAL CELL. 43 in the protoplasm appear to be phosphorized, a condition which i& of importance as showing the genetic connection between the cell nucleus rich in phosphorus and the protoplasm. The extent to which the proteids occur in the young generative cells has not been sufficiently investigated; nevertheless these substances occur habitually and in significant amounts in certain epithelium and glandular cells. Difficultly soluble protein sub- stances are also found' in dead cells or in organs rich in cells which act like coagulated albuminous bodies when treated with the ordinary reagents. In cases in which the protoplasm is surrounded by an outer, con- densed layer or a cell membrane, this envelope seems to consist of albumoid substances. In a few cases — and these, according to BONDERS, answer for the primary animal cell membrane — these substances seem to be closely related to elastin; in other cases, on the contrary, they seem rather to belong to the keratin group. The chemical processes by which these albumoid substances are formed from the albuminous bodies or proteids of the protoplasm are unknown. The occurrence of phosphorized organic combinations in all protoplasm is without doubt of the greatest importance for the functional task, as also for the development of the cell. Of the phosphorized combinations in the cell there are at least two chief groups. To one belongs lecithin, and to the other nuclein, the latter occurring partly in the nucleoalbumin, and forming a part of the chief constituents of the cell nucleus. Lecithin. This body is, according to the investigations of STRECKER, HUNDESHAGEN, and GILSON, an ether-like combination of glycerophosphoric acid substituted by fatty acid radicals, with a base, cholin. Therefore there may be different lecithins accord- ing to the fatty acid contained in the lecithin molecule. One of these — distearyllecitliin — has been closely studied by HOPPE- SEYLER and DIACONOW : C,,H,0NPO, = HO.(CH,)8N.CaH..O(OH)PO.O.C,H,: (O..H..OJ.. In agreement with this, if lecithin be boiled with baryta-water it yields fatty acids, glycerophosphoric acid, and cholin. It is only slowly decomposed by dilute acids. Besides small quantities of 44 PHYSIOLOGICAL CHEMISTRY. glycerophosphoric acid (perhaps also distearylglycerophosphoric acid) we have large quantities of free phosphoric acid split off. GLYCEROPHOSPHORIC ACID (HO)2P0.0.03H5(OH)2 is a bibasic -acid, which probably only occurs in the animal fluids and tissues as splitting product of lecithin. The CHOLIN, which seems to be identical with the bases SINKALIN (in mustard -seed) and AMANITIN (inagaricus muscarius), has the formula HO. N(CH3)3.C2H4.OH and is therefore considered as trimethylethoxylium hydrate. Cholin ac- cording to BEIEGER is not identical with the base, NEURIN, prepared by LIEBREICH as a decomposition product from the brain, which is considered as trimethylvinylium hydrate, HO.N(CH3)3.C2H3. The •combination of cholin with hydrochloric acid gives with platinum chloride a crystalline double combination which is easily soluble in water, insoluble in alcohol and ether, and which crystallizes in six- sided orange-colored plates. This combination is used in detecting this base. Lecithin occurs, as HOPPE-SEYLER has especially shown, widely diffused in the vegetable and animal kingdoms. According to this investigator, it occurs also in many cases in combination with other bodies, such as albuminous bodies, haemoglobin, and others. Lecithin, according to HOPPE-SEYLER, is found in nearly all animal and vegetable cells thus far studied, and also in nearly all fluids. It is specially abundant in the brain, nerves, fish-eggs, yolk of the -egg, electrical organs of the gymnotus electricus, semen and pus, and also in the muscles and blood-corpuscles, blood-plasma, lymph, milk, and bile, as well as in other animal juices and liquids. Lecithin is also found in pathological tissues or liquids. Lecithin may be obtained in grains or warty masses composed of small crystalline plates by strongly cooling its solution in strong alcohol. In the dry state it has a waxy appearance, is mouldable and soluble in alcohol, especially on heating (to 40°-50° C.); it is less soluble in ether. It is dissolved also by chloroform, carbon disulphide, benzol, and fatty oils. It swells in water to a pasty mass which shows under the microscope slimy, oily drops and threads, so-called myelin forms (see Chap. X). On warming this swollen mass or the concentrated alcoholic solution, decom- position takes place with the production of a brown color. On allowing the solution or the swollen mass to stand, decomposition THE ANIMAL CELL. 45 takes place and the reaction becomes acid. In putrefying lecithin yields glycerophosphoric acid and cholin; the latter further decom- poses with the formation of methylamin, ammonia, carbon dioxide, and marsh-gas (HASEBROEK). If dry lecithin be heated it decom- poses, takes fire and burns, leaving a phosphorized coke. On fusing with caustic alkali and saltpetre it yields alkali phosphates. Lecithin is easily carried down during the precipitation of other compounds such as the albuminous bodies, and may therefore very greatly change the solubilities of the latter. Lecithin combines with acids and bases. The combination with hydrochloric acid gives with platinum chloride a double salt which is insoluble in alcohol, soluble in ether, and which contains 10.2$ platinum. It may be prepared tolerably pure from the yolk of the hen's egg by the following methods, as suggested by HOPPE-SEYLER and DIACONOW: The yolk, deprived of albumin, is extracted with cold ether until all the yellow color is removed. Then the residue is extracted with water at 50-60° C. After the evaporation of the alcoholic extract at 50-60° C., the syrupy matter is treated with ether and the insoluble residue dissolved in as little alcohol as pos- sible. On cooling this filtered alcoholic solution to — 5° to— 20° C. the lecithin gradually separates in small grains. According to GILSON, a new portion of lecithin may be obtained from the ether used in extracting the yolk by dissolving the residue after the evaporation of the ether in petroleum ether and then shaking this solution with alcohol. The petroleum ether takes the fat, while the lecithin remains dissolved in the alcohol and may be obtained therefrom rather easily by using the proper precautions. The detection and the quantitative estimation of lecithin in animal fluids or tissues is based on the solubility of the lecithin (at 50-60° C.) in alcohol-ether, by which the phosphoric acid or glycero- phosphoric acid salts which may be present at the same time are not dissolved. The alcohol-ether extract is evaporated, the residue dried and burnt with soda and saltpetre. Phosphoric acid is formed from the lecithin, and it can be detected and quantitatively esti- mated. The distearyllecithin yields 8.798^ P20B. This method is, however, not exactly correct, for it is possible that other phosphor- ized organic combinations, such as jecorin (see Chapter VI), may have passed into the alcohol-ether extract. For the detection of lecithin, boiling with baryta-water and the preparation of the double platinum salt of cholin is sufficient. The study of the second phosphorized constituents of the cell. the nuclein, is generally pursued with the study of the cell nucleus. 46 PHYSIOLOGICAL CHEMISTRY. The Cell Nucleus, as far as investigated, contains nuclein as chief constituent. Nucleins. By this name HOPPE-SEYLER and MIESCHER desig- nated the chief constituent of the nucleus of the pus cell first isolated by them. Since that time, as by continuous investigations the same body is found very widely diffused in the animal and vegetable kingdoms, especially in organs rich in cells, we now desig- nate as nuclein a number of phosphorized bodies which are partly obtained as splitting products of the nucleoalbumins, and partly the chief constituents of the cell nucleus. According to HOPPE-SEYLER, these bodies may be divided into three groups. The first, to which belong the nuclein of yeast, pus, nucleated red blood-corpuscles, and probably the cell nucleus in general, on boiling with acids yields as splitting products albumin- ous bodies, xanthin bodies, and phosphoric acid. To the second group, which yields as splitting products albumin and phosphoric acid, belong the nuclein of the yolk of the egg and casein — in other words, from the nucleo-albumins in general, and to the third group, which gives as splitting products only phosphoric acid and hypoxan- thin, belongs only the nuclein of the sperm of the salmon. LIEBER- MANN has split off metaphosphoric acid from the nuclein of yeast, and he has also found that the metaphosphoric acid gives a com- bination with albumin which acts like a nuclein of the second group. POHL has also come to the same result in so far as he has been able to prepare a combination of metaphosphoric acid with serum albumin and albumose which is similar to nuclein. LIEBER- MANK therefore considers nuclein as a combination between albumin and metaphosphoric acid. The xanthin bodies, which, according to KOSSEL, are decomposition products of the nucleins, according to LIEBERMANK probably come from admixture. That we find different constitutions for nucleins of different origin is not remarkable. A variation of 3.2-9.6$ in the amount of phosphorus has been found in different nucleins. Under such conditions, and as the nuclein question is at present doubtful, it is hardly of any use to give the results of the elementary analyses of the different nucleins. The nucleins are colorless, amorphous, insoluble, or only slightly soluble in water. They are insoluble in alcohol and ether. Thev THE ANIMAL CELL. 47 are more or less easily dissolved by alkalies ; in dilute mineral acids they are insoluble or dissolve with difficulty. The nucleins are not dissolved by pepsin-hydrochloric acid, or only slightly by its continuous action. The nucleins containing albumin answer to the biuret test and MILLON'S reaction. With dilute mineral acids at ordinary temperature they give off (at least for nuclein from yeast or yolk of egg) metaphosphoric acid. On boiling with caustic alkali they decompose and alkali phosphates are formed. On burning they leave an acid-reacting, difficultly-burnt coke which contains metaphosphoric acid. On fusing with saltpetre and soda they give alkali phosphates. To prepare nucleins from nucleoalbumins casein is the best ma- terial to employ. This is first dissolved in water containing about 2 p.m. HC1, the filtered solution treated with pepsin and digested at the temperature of the body. After a little time a precipitate con- sisting of nuclein appears, which is purified by repeated solution in water with the aid of the smallest quantity of alkali, and by repre- cipitating with acid, washing with water, and extracting with alcohol and ether. From cells or tissues first remove the chief mass of the albumins by the artificial digestion with pepsin-hydrochloric acid, digest the residue with very dilute ammonia, filter, and precipitate with hydrochloric acid. This precipitate is now digested with artificial gastric juice and treated as above described. In detecting nuclein the same method is used, and the last product is fused with soda and saltpetre, and phosphoric acid tested for in the melted mass. Naturally the phosphate lecithin (and jecorin) must first be removed by acid, alcohol, and ether respectively. No exact methods are known for the quantitative estimation of the nucleins in organs and tissues. Among the decomposition products of the nucleins the so- called xanthin bodies are especially of great interest. Although LIEBERMANN, in opposition to the views of KOSSEL, considers these bodies not as real decomposition products of nuclein, but only as admixtures, yet until this question is settled more defi- nitely, and since the xanthin bodies stand in close relationship to the cell nucleus, it is perhaps most proper to speak of these bodies in connection with the cell nucleus and the nucleins. Xanthin Bodies. With this name we designate a group of bodies consisting of carbon, hydrogen, nitrogen, and in most cases 48 PHYSIOLOGICAL CHEMISTRY. also of oxygen, which, by their constitution, show a relationship not only among themselves, but also with uric acid. These bodies are xanthin, hypoxanthin, guanin, adenin, heteroxanthin, para- xanthin, and carnin. The bodies THEOBROMIN and THEOPHYLLIN (both dimethyl xanthin) and CAFFEIK (trimethyl xanthin) occur- ring in the vegetable kingdom also belong to this group. The relation of these bodies to one another is shown in the following list: Uric acid C6H4N4O3 Xanthin C5H4N4O3 Hypozanthin C6H4N4O Guanin C5H5N6O Adenin C5H5N5 Heteroxanthin C6H6N4O2 Paraxanthin C7H8N4Oa Carnin C7H8N4O3 Guanin may be converted into xanthin, and adenin into hypo- xanthin, by nitrous acid, also by putrefaction. Carnin is converted into hydrobromic-acid hypoxanthin by bromine-water. Adenin, as is shown by its formula, is a polymeric substance of hydrocyanic acid, and on its decomposition with alkali yields alkali cyanides. The relationship of these bodies to cyanogen is also shown by GAUTIEE, who prepared xanthin synthetically from hydrocyanic acid. The significance of the xanthin bodies as decomposition prod- ucts of the cell nucleus and of nuclein was first pointed out by KOSSEL, who discovered the two bodies adenin and theophyllin, and his researches have greatly contributed towards the knowledge of xanthin products. In those tissues in which, as in the glands, the cells have kept their original state, the xanthin bodies are not found free, but in combination with other atomic groups (nu- cleins). In such tissue, on the contrary, as in muscles, which are poor in cell nuclei, the xanthin bodies are found in the free state. If the xanthin bodies, as suggested by KOSSEL, stand in close rela- tionship to the cell nucleus, it is easy to understand why the mass of these bodies is so greatly increased when large quantities of nu- cleated cells appear in such places as were before relatively poorly endowed. As an example of this we have in leucaemia blood extremely rich in leucocytes. In such blood KOSSEL found 1.04 THE ANIMAL CELL. 49 p. m. xanthin bodies, against only traces in the normal blood. That the xanthin bodies are also intermediate steps in the forma- tion of urea or uric acid in the animal organism, as will be shown later (see Chapter XIV), is probable. Those xanthin bodies which have thus far been obtained in the decomposition of the nucleins, or generally from cells, or from tissue rich in cell nuclei, and on this account will be described here, are xanthin, hypoxanthin, guanin, and adenin, all of which are found in the vegetable kingdom (SCHULZE and BOSSHAKD, KOSSEL, BAGINSKY). The carnin, which has only been found in meat extracts (WEIDEL) and in the flesh of certain fishes, as also in the bodies paraxanthin and heteroxanthin (SALOMON), which are only found in urine, will be described in their proper chapters, namely, IX and XIV. TVTTT PIT • O ~\TTT Xanthin, O.H.N.O. = ' '/ >0<> (E' Fl8CHEE)> is found in the muscles, liver, spleen, pancreas, kidneys, testicles, carp-sperm, thymus, and brain. It occurs in the smallest amounts as a physiological constituent of urine, and it has been found rarely as a urinary sediment or calculus. It was first observed in such a stone by MAECET. Xanthin is found in larger amounts in a few varieties of guano (Jarvis guano). Xanthin is amorphous, or forms granular masses of crystals. It is very slightly soluble in water, in 14,151-14,600 parts at -f 16° C., and in 1300-1500 parts at 100° C. (ALMEN). It is insoluble in alcohol or ether, but is dissolved by alkalies or acids. With hydrochloric acid it gives a crystalline, difficultly-soluble combination. Xanthin dissolved in ammonia gives with silver nitrate an insoluble, gelatinous precipitate of xanthin silver. This precipitate is dissolved by nitric acid, and by this method an easily- soluble double combination is formed. A watery xanthin solution is precipitated on boiling with copper acetate. At ordinary tem- peratures xanthin is precipitated by mercuric chloride and by am- moniacal lead acetate. When evaporated to dryness in a porcelain dish with nitric acid xanthin gives a yellow residue, which turns, on the addition of caustic soda, first red, and, after heating, purple red. If we add some chloride of lime to some caustic soda in a porcelain dish 50 PHYSIOLOGICAL CHEMISTRY. and add the xanthin to this mixture, at first a dark green and then quickly a brownish halo forms around the xanthin grains and then disappears (HOPPE-SEYLEE). If xanthin be warmed in a small vessel on the water-bath with chlorine-water and a trace of nitric acid and evaporated to dryness, when the residue is exposed under a bell-jar to the vapors of ammonia a red or purple-violet color is produced (WEIDEI/S reaction). It is still a question whether en- tirely pure xanthin will give this reaction. Hypoxanthin or SAEKIN, CBH4N40. This body is found in the tissues containing xanthin. It is especially abundant in the sperm of salmon and carp. It occurs also in the marrow. In normal urine it appears in very small quantities, and it seems also to be found in milk. It is found in rather significant quantities in the blood and urine in leucocythaemia. Hypoxanthin forms very small colorless crystalline needles. It is more soluble than xanthin. It dissolves in 300 parts cold and 78 parts boiling water. It is nearly insoluble in alcohol, but it is dissolved by alkalies and acids. The combination with hydrochloric acid crystallizes and is more soluble than the corresponding xanthin combination. It acts in ammoniacal solution like xanthin with silver nitrate. The silver combination of hypoxanthin dissolves with difficulty in boiling nitric acid of 1.1 sp. gr., and on cooling the double combination separates as crystalline needles. Treated like xanthin with nitric acid, hypoxanthin gives a nearly colorless residuum which does not become red by heating with alkali. Hypo- xanthin does not give WEIDEI/S reaction. After the action of hydrochloric acid and zinc a hypoxanthin solution becomes first ruby-red and then brownish red in color on the addition of an excess of alkali (KOSSEL). NH.CH-.C.NH Guamn, C5H5N60 = NH . ^ NH c-N> Ghtanin is found in organs rich in cells, such as the liver, spleen, pancreas, testi- cles, and in salmon-sperm. It is further found in the muscles (in very small amounts), in the scales and in the air-bladder of certain fishes as iridescent crystals of guanin lime; in the retina epithelium of fishes, in guano, and in the excrement of spiders it is found as chief constituent. Under pathological conditions it has been found in oar leucocythaemic blood, and in the muscles, ligaments, and articula- tions of pigs with guanin gout. Guanin is a colorless, ordinarily amorphous powder which may be obtained as small crystals by allowing its solution in concentrated ammonia to spontaneously evaporate. It is nearly insoluble in water, alcohol, and ether. It is easily dissolved by mineral acids and alkalies, but it dissolves with great difficulty in ammonia. The silver combination dissolves with difficulty in boiling nitric acid, and on cooling the double combination crystallizes. Guanin acts like xanthin in the nitric-acid test, but gives with alkalies on heating a stronger bluish-violet color. A warm solution of guanin hydrochloride gives with a cold saturated solution of picric acid a yellow precipitate consisting of silky needles (CAPRANICA). With a concentrated solution of potassium chromate a guanin solution gives a crystalline, orange-red precipitate, and with a concentrated solution of potassium ferricyanide a yellowish brown, crystalline precipitate (CAPRANICA). Adenin, C6H6N6, was first found by KOSSEL in the pancreas gland. It occurs in greatest quantities in the sperm of the carp and in the thymus (KOSSEL and SCHMIDLER). It also occurs in the liver, spleen, lymphatic glands, and kidneys (not in muscles). It has been observed in leucocythaemic urine. Adenin crystallizes in long needles. It dissolves in cold water with difficulty (1086 parts), but easily in warm. Pure adenin dis- solves slightly in boiling alcohol, in cold not at all ; impure adenin is, however, dissolved by cold alcohol. It is insoluble in ether. Adenin is easily dissolved by acids and alkalies. In dilute ammo- nia it dissolves with more difficulty than hypoxanthin, but more easily than guanin. The silver combination dissolves with difficulty in boiling nitric acid and crystallizes on cooling. The nitric-acid test and WEIDEL'S reaction act the same as with hypoxanthin. The same is true for its behavior with hydrochloric acid and zinc with addition of alkali. The principle for the preparation, detection, and the quantitative estimation of the four above-described xanthin bodies is, according to KOSSEL and KOSSEL and SCHINDLER, as follows : The finely-divided organ or tissue is boiled for three or four hours with sulphuric acid of about 5 p. m. The filtered liquid is freed from albumin by lead 52 PHYSIOLOGICAL CHEMISTRY. acetate, and the new filtrate is treated with sulphuretted hydrogen to remove the lead, again filtered, concentrated, and, after adding an excess of ammonia, precipitated with silver nitrate. The silver combination (with the addition of some urea to prevent nitrification) is dissolved in not too large a quantity of boiling nitric acid of sp. gr. 1.1, and this solution filtered boiling hot. On cooling the silver xanthin remains in the solution, while the double combination of guanin, hypoxanthin, and adenin crystallizes. The xanthin silver may be removed from the filtrate by the addition of ammonia, and the xanthin set free by means of sulphuretted hydrogen. The three above-mentioned silver nitrate combinations are decomposed in water with ammonium sulphide and heat; the silver sulphide is filtered, the filtrate concentrated, saturated with ammonia, and digested on the water-bath. The guanin remains undissolved,. while the other two bases pass into solution. A part of the guanin is still retained by the silver sulphide, and may be liberated by boiling it with dilute hydrochloric acid and then saturating the filtrate with ammonia. When the above filtrate, containing the adenin and hypoxanthin, which has been, if necessary, freed from ammonia by evaporation, is allowed to cool, the adenin separates, while the hypoxanthin remains in solution. The chief points in the above method are used for the quantitative esti- mation of the xanthin bodies. If the solution of adenin and hypoxanthin is evaporated to dryness, the residue weighed, and the amount of nitrogen determined, from this determination and from the amount of nitrogen in hypoxanthin (41.17$) and in adenin (51.87$) the quantity of each of these bodies may be calculated. In the generative animal cells, and especially in those which develop embryonic tissue, CL. BERNARD and HENSEK have dis- covered a carbohydrate, the glycogen. According to HOPPE- SEYLER it seems to be a never-failing constituent of the cells as soon as they show embryonic movements, and he found this carbo- hydrate in the white blood-corpuscles but not in the developed motionless pus-corpuscles. The relationship which exists between the consumption of glycogen and muscular work (see Chapter IX) leads us to suppose that such a consumption takes place in the movements of the animal protoplasm. On the other side the widely-diffused occurrence of glycogen in embryonic tissues, as also its occurrence in pathological swellings and in abundant cell- formations, seems of the greatest importance in the formation and development of the cell. In grown animals the glycogen is found in the muscles and THE ANIMAL CELL. 53 certain other organs, foremost of these being the liver; it will therefore be more thoroughly described in connection with this organ (Chapter VI). Another body or more correctly a group of bodies which occur very widely diffused in the animal and vegetable kingdoms and habitually in the cells are the cholesterines, whose best-known representative is the ordinary cholesterin, specially known as the chief constituent of certain biliary calculi and occurring in large quantities in the brain and nerves. It is hardly to be admitted that this body has any direct importance in the life and the develop- ment of the cell. It is more probable, as HOPPE-SEYLER suggests, that cholesterin is a splitting product appearing in the general life- processes of the cells. Also according to HOPPE-SEYLER the fat, which does not constantly appear in the cell, has nothing to do with the general process of life. Mineral bodies are also never-failing constituents of the cell. These minerals are potassium, sodium, calcium, magnesium, iron, phosphoric acid, and chlorine. In regard to the alkalies we find in general in the animal organism that the sodium combinations are more abundant in the fluids, the potassium combinations occur chiefly in the form-constituents and in the protoplasm. Corre- sponding to this the cell contains potassium, chiefly as phosphate, while the sodium and chlorine combinations occur less abundantly. According to the ordinary views the potassium combinations, espe- cially the potassium phosphate, are of the greatest importance for the life and development of the cell, even though we ^do not know the nature of the importance. At least we must not overlook the fact that a part of the phosphoric acid which is obtained from the cell or tissue rich in cells may originate from the nuclein and leci- thin in the process of ashing. Also the iron, which often occurs in the ash as ferric phosphate, seems, at least in part, to be formed from the nucleo-albumin. The habitual occurrence of earthy phosphates in all cells and tissues, together with the difficulty or almost impossibility of separating these bodies from the protein substances without decomposition, leads us to suppose that these mineral bodies are indeed, though their role is still unknown, of the greatest importance for the life of the cells and the chemical processes which accompany their evolution. CHAPTER IV. THE BLOOD. THE blood is to be considered from a certain standpoint as a fluid tissue, and it consists of a transparent liquid, the blood- plasma, in which an immense number of solid particles, the red and colorless blood-corpuscles (and the blood-tablets) are suspended. Outside of the organism the blood, as is well known, coagulates more or less quickly; but this coagulation is accomplished gener- ally in a few minutes after leaving the body. All varieties of blood do not coagulate with the same degree of rapidity. Some coagulate more quickly, others more slowly. Among the varieties of blood thus far investigated the blood of the horse coagulates most slowly. The coagulation may be more or less retarded by quickly cooling; and if we allow equine blood to flow directly from the vein into a glass cylinder which is not too wide and which has been cooled, and let it stand at 0° C., the blood may be kept fluid for several days. An upper, amber-yellow layer of plasma gradually separates from a lower, red layer composed of blood-corpuscles with only a little plasma. Between these we observe a whitish-gray layer, which consists of white blood-corpuscles. The plasma thus obtained and filtered is a clear amber-yellow alkaline liquid which remains fluid for some time when kept at 0° C., but soon coagulates at the ordinary temperature. The coagulation of the blood may be prevented in other ways. After the injection of peptone or, more correctly, albumose solu- tions into the blood (in the living dog), the blood does not coagulate on leaving the veins (FANO, SCHMIDT-MULHEIM). The plasma obtained from such blood by means of centrifugal force is called "peptone-plasma." The coagulation of the blood of warm- 54 THE BLOOD. 55 blooded animals is prevented by the injection of an effusion of the mouth of the officinal leech into the blood-current (HAYCRAFT). If the blood-circulation of a dog is cut off between the liver and intestines and the blood allowed to flow only through the head and the viscera of the thoracic cavity, the coagulation of the blood is destroyed (PAWLOW, BOHR). If we alloM the blood to flow directly, while we stir it, into a neutral salt solution — best a satu- rated magnesium sulphate solution (1 vol. salt solution and 3 vols. blood) — we obtain a mixture of blood and salt which re- mains uncoagulated for several days. The blood-corpuscles which, because of their adhesiveness and elasticity, would otherwise pass easily through the pores of the filter-paper are made solid and stiff by the salt, so that they may be easily filtered. The plasma thus obtained, which does not coagulate spontaneously, is called " salt- plasma" On coagulation there separates in the previously fluid blood an insoluble or a very difficultly-soluble albuminous substance, fibrin. When this separation takes place without stirring the blood coagu- lates to a solid mass which, when carefully severed from the sides of the vessel, contracts, and a clear, generally yellow-colored liquid, the Uood-serum, exudes. The solid coagulum which incloses the blood-corpuscles is called the blood-clot (placenta sanguinis). If the blood is beaten during coagulation, the fibrin separates in elastic threads or fibrous masses, and the defibrinated blood which separates is sometimes called cruor,1 and consists of blood-cor- puscles and blood-serum. The defibrinated blood consists of blood-corpuscles and serum, while the uncoagulated blood consists of blood-corpuscles and blood-plasma. The essential chemical difference between blood- serum and blood-plasma is that the blood-serum does not contain the mother-substance of fibrin, the fibrinogen, which exists in the blood-plasma, and the serum is proportionally richer in another body, the fibrin ferment (see page 58). 1 The name CRUOR is used in different senses. We sometimes understand thereby only the blood when coagulated to a red solid mass, in other cases the blood-clot after the separation of the serum, and lastly the sediment con- sisting of red blood-corpuscles which is obtained from defibrinated blood by means of centrifugal force or by letting it stand. 56 PHYSIOLOGICAL CHEMISTRY. I. Blood-plasma and Blood-serum. The Blood-plasma. In the coagulation of the blood a chemical transformation takes place in the plasma. A part of the albumins separates as insoluble fibrin. The albuminous bodies of the plasma must therefore be first described. They are fibrinogen, serum globulin, and serum albumin. Fibrinogen occurs in blood-plasma, chyle, lymph, and in certain transudations and exudations.1 It has the general properties of the globulins, but differs from other globulins as follows: In a moist condition it forms white flakes which are soluble in dilute common salt solutions, and which easily conglomerate into tough, elastic masses or lumps. The solution in NaCl of 5-10$ coagulates on heating to -j- 52° to 55° C., and the faintly alkaline or nearly neutral weak salt solution coagulates at -f- 56° C., or at exactly the same tem- perature at which the blood-plasma coagulates. Fibrinogen solu- tions are precipitated by an equal volume of a saturated common salt solution, and are completely precipitated by adding an excess of NaCl in substance (thus differing from serum globulin). It differs from myosin of the muscles, which coagulates at about the same temperature, and from other albuminous bodies, in the prop- erty of being converted into fibrin under certain conditions. Fi- brinogen has a strong decomposing action on hydrogen peroxide. The fibrinogen may be easily separated from the salt-plasma by precipitation with an equal volume of a saturated NaCl solution. For further purification the precipitate is pressed, redissolved in an 8$ salt solution, the filtrate precipitated by a saturated salt solution as above, and after precipitating in this way three times, the precipitate at last obtained is pressed between filter-paper and finely divided in water. The fibrinogen dissolves with the aid of the small amount of NaCl contained in itself, and the solution may be made salt-free by dialysis with very faintly alkaline water. From transudations we ordinarily obtain a fibrinogen which is strongly 1 The question as to the occurrence of other fibrinogens (WOOLDRIDGE) will be spoken of in connection with the complete discussion of the coagula- tion of the blood. (See further on.) THE BLOOD. 67 contaminated with lecithin and which can hardly be purified with- out decomposing. The method for the detection and quantitative estimation of fibrinogen in a liquid is based on its property of yielding fibrin on the addition of a little blood, of serum, or of fibrin ferment. The fibrinogen stands in close relation to its transformation- product, the fibrin. Fibrin is the name of that albuminous body which separates on the so-called spontaneous coagulation of blood, lymph, and trans- udations, as also in the coagulation of a fibrinogen solution after the addition of serum or fibrin ferment (see below). If the blood is beaten during coagulation, the fibrin separates in elastic fibrous masses. The fibrin of the blood-clot may be beaten to small, less elastic, and not particularly fibrous lumps. The typical, fibrous, and elastic white fibrin, after washing, stands in regard to its solubility close to the coagulable albuminous bodies. It is insoluble in water, alcohol, or ether. It expands in hydro- chloric acid of 1 p. m., as also in caustic potash or soda of 1 p. m., to a gelatinous mass, which dissolves at the ordinary temperature only after several days, but at the temperature of the body it dis- solves more readily but still slowly. The fibrin expands in a 5- 10$ solution of common salt or saltpetre, but only dissolves in the presence of contaminating enzymes or by putrefaction. Fibrin decomposes hydrogen peroxide, but this property is destroyed by heating or by the action of alcohol. What has been said of the solubility of fibrin relates only to the typical fibrin obtained from the arterial blood of mammalia or man by whipping and washing first with water and with common-salt solution, and then with water again. The blood of various kinds of animals yields fibrin with somewhat different properties and of varying purity, and likewise blood from different parts of the body may yield fibrim with unlike solubilities ( DENIS). The fibrin obtained by beating the blood and purified as above described is always contaminated by enclosed blood-corpuscles or remains thereof, and also by lymphoid cells. It can only be obtained pure from filtered plasma or filtered trausudations. For the pure preparation, as well as for the quantitative estimation of fibrin, the spontaneously coagulating liquid is at once, or the non-spontaneously coagulating liquid only after the addition of blood-serum or fibrin 58 PHYSIOLOGICAL CHEMISTRY. ferment, thoroughly beaten with a glass rod or whale-bone, and the separated coagulum is washed first in water, and then with a 5$ common-salt solution, and again with water, and lastly extracted with alcohol and ether. A pure fibrinogen solution may be kept at the ordinary tempera- ture until putrefaction begins without showing a trace of fibrin coagulation. But if to this solution we add a water-washed fibrin clot or a little blood-serum, it immediately coagulates and may yield perfectly typical fibrin. The transformation of the fibro- gen into fibrin requires the presence of another body contained in the blood-clot and in the serum. This body, whose importance in the coagulation of fibrin was first observed by BUCHANAN, was later rediscovered by ALEXANDER SCHMIDT, and designated "fibrin ferment." The nature of this enzymotic body has not been ascer- tained. According to the investigations of GAMGEE, LEA, and GREEN and HALLIBURTON, the " fibrin ferment " seems to be a substance of the nature of the globulins. According to HALLI- BURTON, it is a body derived from the lymphoid cells, a special globulin, " cell-globulin" which differs from serum globulin partly by fibrino-plastic properties and partly by having another temperature of coagulation (-J- 60° C., or somewhat higher in a solution contain- ing 10$ JSTaCl). The so-called fibrin ferment corresponds to the enzymes in that only the very smallest amounts of it are required for action, and further that on heating the solution it becomes in- active. The isolation of the fibrin ferment has been tried in several ways. Ordinarily it may be prepared by the following method proposed by ALEX. SCHMIDT : Precipitate the serum or defibrinated blood with 15-20 vols. of alcohol and allow it to stand a few months. The precipitate is then filtered and dried over sulphuric acid. The ferment may be extracted from the dried powder by means of water. If a fibrinogen solution containing salt, as above described, is treated with a solution of " fibrin ferment," it coagulates at the ordinary temperature more or less quickly and yields a typical fibrin. Besides the fibrin ferment the presence of neutral salts is necessary, for without them ALEX. SCHMIDT has shown the coagu- lation of fibrin does not take place. The amount of fibrin obtained THE BLOOD. 59 on coagulation is always smaller than the amount of fibrinogen from which the fibrin is derived, and we always find a small amount of globulin substance in the solution. It is therefore not improbable that the coagulation of fibrin, in accordance with the views of DENIS, is a splitting process in which the soluble fibrinogen is split into an insoluble albuminous body, the fibrin, which forms the chief mass, and a soluble globulin substance, which is only formed in small amounts. The globulin substance, which is called "fibrin globulin " by the author, coagulates at -|- 64° C. and has the following com- position : 0 52.70$ ; H 6.98$ ; N 16.06$. The coagulation of the blood consists chiefly in the conversion of the fibrinogen of the plasma into fibrin. The coagulation of the blood is a much more complicated process than the coagulation of a fibrinogen solution, inasmuch as the first involves other important questions, as, for instance, the reason for the blood remaining fluid in the body, the origin of the fibrin ferment, and the importance of the form-elements in the coagulation. A fuller discussion of the various hypotheses and theories concerning the coagulation of the blood must therefore be given later. Serum Globulin, also called paraglobulin (KiJHNE), fibrino- plastic substance (ALEX. SCHMIDT), serum casein (PANUM), fibrine soluble (DENIS), occurs in the plasma, serum, lymph, transudations and exudations, in the white and red corpuscles, and probably in many animal tissues and form-elements, though in small quantities. It is also found in the urine in many diseases. Serum globulin has the general properties of the globulins. In a moist condition it forms a snow-white flaky mass neither tough nor elastic. The essential differences between serum globulin and fibrinogen are the following: Serum-globulin solutions are only incompletely precipitated by adding NaCl to saturation, and not precipitated at all by an equal volume of a saturated common-salt solution. The coagulation temperature is, with 5-10$ NaCl in solution, -j- 75° C. It is completely precipitated by MgS04 in sub- stance added to saturation, as also by an equal volume of a sat- urated solution of ammonium sulphate. The specific rotary power, according to FREDEEICQ, for serum globulin (from-ox blood) solu- tions containing salt is 7.25 17.70 0.477 0.400 19.543 , 7.38 16.23 0.660 0.430 21.360 7.38 17.43 0.479 0.399 19.602 7.36 16.78 0.580 0.480 20.680 7.39 16.09 0.400 0.590 21.440 7.10 16.21 0.590 0.430 20.690 0 7*7 7.19 16.45 0.857 0.335 22.500 0 19 (HOPPE-SEYLER.) (JAQUET.) . (KoSSEL.) (ZlNOFFSKY.) (HfJFNER.) (OTTO.) (HtJFNER.) (HOPPE-SEYLER. ) 197 (JACQUET.) The question whether the amount of phosphorus in the haemo- globin from birds exists as a contamination or as a constituent has not been decided. In the haemoglobin from the horse (ZINOFFSKY), the pig, and the ox (HuF^ER) we have 1 atom of iron to 2 atoms of sulphur, while in the haemoglobin from the dog (JACQUET) the rela- tion is 1 to 3. From the data of the elementary analysis, as also from the amount of loosely-combined oxygen, HUF^ER has calculated the molecular weight of dog-haemoglobin as 14,129 and the formula CM8H109BN1MFeSa0181. The molecular weight is therefore very high. The haemoglobin from various kinds of blood not only show a diverse constitution, but also a different solubility and crystalline form, and a varying quantity of water of crystallization, from which we infer that there are several kinds of haemoglobin. Oxyhsemoglobin, which has also been called H^MATOGLOBULIN or H^EMATOCRYSTALLIN, is a molecular combination of haemoglobin and oxygen. For each molecule of haemoglobin 1 molecule of oxy- gen exists; and the amount of loosely-combined oxygen which is united to 1 grm. oxyhaemoglobin (of the dog) has been determined by 70 PHYSIOLOGICAL CHEMISTRY. HUFNER as 1.582 c. c. (at 0° C. and 760 m.m. Hg). The ability of haemoglobin to take up oxygen seems to be a function of the iron it contains, and when this is calculated as about 0.33-0.40$, then 1 atom of iron in the haemoglobin corresponds to about 2 atoms = 1 molecule of oxygen. The combination of haemoglobin with oxygen is, as has been stated, loose and dissociatable, and the quantity of oxygen taken up by a haemoglobin solution depends upon the pressure of the oxygen at that temperature. If this latter be decreased by means of a vacuum, especially on gently heating or by passing some indifferent gas through the solution, all of the oxygen may be expelled from an oxyhaemoglobin solution so that only haemoglobin remains. The reverse of this is true of a haemo- globin solution which by its remarkable attraction for oxygen may be converted into oxyhaemoglobin. Oxyhaemoglobin is generally considered as a weak acid. Oxyhaemoglobin has been obtained in crystals from several varieties of blood. These crystals, first observed by REICHERT and FUNKE, are blood-red, transparent, silky, and may be 2-3 m.m. long. The oxyhaemoglobin from squirrel's blood crystallizes in six- sided plates of the hexagonal system; the other varieties of blood yield needles, prisms, tetrahedra, or plates which belong to the rhombic system. The quantity of water of crystallization varies between 3-10$ for the different oxyhaemoglobins. When com- pletely dried at a low temperature over sulphuric acid the crys- tals may be heated to 110°-115° C. without decomposing. At higher temperatures, somewhat above 160° C., they decompose, giving an odor of burnt horn, and leave, after complete combus- tion, an ash consisting of oxide of iron. The oxyhaemoglobin crystals from difncultly-crystallizable kinds of blood, for exam- ple from such as ox's, human, and pig's blood, are easily soluble in water. The oxyhaemoglobin from easily- crystallizable blood, as from that of the horse, dog, squirrel, and guinea-pig, are soluble with difficulty in the order above given. The oxyhaemoglobin dissolves more easily in a very dilute solution of alkali carbonate than in pure water, and this solution may be kept. The presence of a little too much alkali causes the oxyhaemoglobin to quickly decompose. The crystals are insoluble without decolorization in absolute alcohol. According to NENCKI, it is converted THE BLOOD. 71 into an isomeric or polymeric modification-, called by him parahcemo- globin. Oxyhaemoglobin is insoluble in ether, chloroform, benzol, and carbon disulphide. A solution of oxyhaemoglobin in water is not precipitated by many metallic salts, but is precipitated by sugar of lead. On heat- ing the watery solution it decomposes at 60° to 70° C., and it splits off albumin and haematin. It is also decomposed by acids, alkalies, and many metallic salts. It gives the ordinary reactions for albu- min with the albumin reagents which first decompose the oxyhaemo- globin with the splitting off of albumin. The oxyhsemoglobin may, when it is gradually oxidized, act as an " ozone exciter " by the disjoining of neutral oxygen, making the oxygen active (PFLUGER). It may also have another relation to ozone, since it has the property of an " ozone transmitter " in that it causes the reaction of certain reagents (turpentine) containing ozone upon ozone reagents such as tincture of guaiacum (SCHON- BEIN, His). According to KOWALEWSKY, it is not ozone but an oxidation-product of turpentine that we have to deal with; but this question requires further proof. A sufficiently dilute solution of oxyhaemoglobin or arterial blood shows a spectrum with two absorption-bands between the FRATJN- HOFER lines D and E. The one band a, which is narrower but darker and sharper, lies on the line D\ the other, broader, less de- fined and less dark band /?, lies at E. These bands can be detected in a. layer of 1 cm. of a 0.1 p. m. solution of oxyhaemoglobin. In a still weaker dilution the band /? first disappears. By increased con- centration of the solution the two bands become broader, the space between them smaller or entirely obliterated, and at the same time the blue and violet part of the spectrum is darkened. The oxyhaemo- globin may be differentiated from other coloring matters having a similar absorption-spectrum by its behavior towards reducing sub- stances. (See below.) A great many methods have been proposed for the preparation of oxyhaemoglobin crystals, but in their chief features they all agree with the following method as suggested by HOPPE-SEYLER : The washed blood-corpuscles (best those from the dog or the horse) are stirred with 2 vols. water and then shaken with ether. After decanting the ether and allowing the ether which is retained by 72 PHYSIOLOGICAL CHEMISTRY. the blood solution to evaporate in an open dish in the air, cool the filtered blood solution to 0° C., add while stirring £ vol. of alcohol also cooled, and allow to stand a few days at — 5° to — 10° C. The crystals which separate may be repeatedly recrystallized by dis- solving in water of about 35° C, cooling and adding cooled alcohol as above. Lastly, they are washed with cooled water containing alcohol (i vol. alcohol) and dried in vacuum at 0° 0. or a lower temperature. According to GSCHEIDLEN'S investigations, oxy- haemoglobin crystals may be obtained from difficulty crystallizable varieties of blood by allowing the blood first to putrify slightly in sealed tubes. After shaking with air by which the blood is again arterialized, proceed as above. For the preparation of oxyhaemoglobin crystals in small quanti- ties from blood easily crystallized, it is often sufficient to stir a drop of blood with a little water on a microscope slide and allow the mixture to evaporate so that the drop is surrounded by a dried ring. After covering with a thin glass, the crystals gradually appear radiat- ing from the ring. These crystals are formed in a surer manner if the blood is first mixed with some water in a test-tube and shaken with ether and a drop of the lower deep-colored liquid treated as above on the slide. Haemoglobin, also called KEDUCED HAEMOGLOBIN or PURPLE CRUORIN (STOKES), occurs only in very small quantities in arterial blood, in larger quantities in venous blood, and is nearly the only blood-coloring matter after suffocation. Haemoglobin is much more soluble than the oxyhaemoglobin, and it can therefore only be obtained as crystals with difficulty. These crystals are as a rule isomorphous to the corresponding oxyhaemo- globin crystals, but are darker, having a shade towards the blue or purple, and are decidedly more pleochromatic. Its solutions in water are darker and more violet or purplish than solutions of oxy- haemoglobin of the same concentration. They absorb the blue and the violet rays of the spectrum in a less marked degree, but strongly absorb the rays lying between C and D. In proper dilution the solution shows a spectrum with one broad, not sharply-defined band between D and E. This band does not lie in the middle between D and E, but is towards the red end of the spectrum, a little over the line D. A haemoglobin solution actively absorbs oxygen from the air and is converted into an oxyhaemoglobin solution. A solution of oxyhaemoglobin may be easily converted into a haemoglobin solution by means of a vacuum, by passing an indiffer- THE BLOOD 73 ent gas through, or by the addition of a reducing substance, as, for example, an ammoniacal ferrotartrate solution (STOKES' reduction- liquid). If an oxyhaemoglobin solution or arterial blood is kept in a sealed tube, we observe a gradual reduction of the oxyhaemo- globin into haemoglobin. If the solution has a proper concentration, a crystallization of haemoglobin may occur in the tube at lower temperatures (HUFNER). Carbon Monoxide Haemoglobin is the molecular combination be- tween 1 mol. haemoglobin and 1 mol. CO. This combination is stronger than the oxygen combination of haemoglobin. The oxygen is for this reason easily driven off by carbon monoxide, and this ex- plains the poisonous action of carbon monoxide, which kills by the expulsion of the oxygen of the blood. Carbon monoxide haemoglobin is formed by saturating blood or a haemoglobin solution with carbon monoxide, and may be obtained as crystals by the same means as oxyhaemoglobin. These crystals -are isomorphous to the oxyhaemoglobin crystals, but are less soluble, more constant, and their bluish-red color is more marked. For the detection of carbon monoxide haemoglobin its absorption spectrum is of the greatest importance. This spectrum shows two bands which are very similar to those of oxyhaemoglobin, but they occur more towards the violet part of the spectrum. These bands do not change noticeably on the addition of reducing substances; this constitutes an important difference between carbon monoxide and oxyhaemo- globin. If the blood contains oxyhaemoglobin and carbon monoxide haemoglobin at the same time, we will obtain on the addition of a reducing substance (ammoniacal ferrotartrate solution) a mixed spectrum originating from the haemoglobin and carbon monoxide haemoglobin. A great many reactions have been suggested for the testing of carbon-monoxide haemoglobin in medico-legal cases. A simple and at the same time a good one is HOPPE-SEYLER'S soda test. The blood is treated with double its volume of caustic-soda solution of 1.3 sp. gr., by which ordinary blood is converted into a dingy brownish mass, which when spread out on porcelain is brown with a shade of green. Carbon-monoxide blood gives under the same conditions a red mass, which if spread out on porcelain shows a beautiful red color. Several modifications of this test have been proposed. in* 17 ER SIT 71 74 PHYSIOLOGICAL CHEMISTRY. Nitric -oxide Haemoglobin is also a crystalline molecular com- bination which is even stronger than the carbon-monoxide haemo- globin. Its solution shows two absorption-bands which are paler and less sharp than the carbon-monoxide haemoglobin bands, and they disappear on the addition of reducing bodies. Haemoglobin forms also a molecular combination with acetylene. It is also claimed that it forms a combination with hydrocyanic acid. Carbon - dioxide Haemoglobin. Haemoglobin forms a molecular combination with carbon dioxide whose spectrum is similar to that of haemoglobin (ToRtip). By the action of carbon dioxide on haemoglobin part of the latter is decomposed with the separation of albumin (BoHR, TORUP), and the combination seems to be rather carbon-dioxide haemochromogen (see below : Hasmochromogen). Methaemoglobin. This name has been given to a coloring matter which is easily obtained from the oxyhaemoglobin as a transforma- tion product and which has been correspondingly found in transuda- tions and cystin fluids containing blood, in urine, in haematuria or haemoglobinuria, also in urine and blood on poisoning with potas- sium chlorate, amyl nitrite or alkali nitrite, and many other bodies. Methaemoglobin does not contain any oxygen in molecular or dissociable combination, but still the oxygen seems to be of im- portance in the formation of methaemoglobin. If arterial blood be sealed up in a tube, it gradually consumes its oxygen and becomes venous, and by this absorption of oxygen a little methaemoglobin is formed. The same occurs on the addition of a small quantity of acid to the blood. By the spontaneous decomposition of blood some methaemoglobin is formed, and by the action of ozone, potas- sium permanganate, potassium ferricyanide, and certain other bodies on the blood an abundant formation of methaemoglobin takes place. According to a few investigators, among others SORBY and JADERHOLM, the methaemoglobin contains more oxygen than oxyhaemoglobin, while according to others, among whom may be mentioned HOPPE-SEYLER, it contains less. HUFNER and OTTO claim that it contains just as much oxygen, but that it is more strongly combined. JADERHOLM and SAARBACH claim that a THE BLOOD. 75 methaemoglobin is first converted into an oxyhaemoglobin and then into a haemoglobin solution by reducing substances, while HOPPE- SEYLER claims that it is converted directly into a haemoglobin solution. Methaemoglobin has the same constitution as oxyhaemoglobin (HiiFNEE and OTTO). It was first shown by them that it crystal- lizes in brownish-red needles, prisms, or six-sided plates. It dissolves easily in water ; the solution has a brown, color and becomes a beautiful red on the addition of alkali. The solution of the pure substance is not precipitated by lead acetate alone, but by lead acetate and ammonia. The absorption-spectrum of a watery or acidified solution of methaemoglobin is very similar to that of haematin in acid solution, but is easily distinguished from the latter since, on the addition of a little alkali and a reducing substance, the former passes over to the spectrum of reduced haemoglobin, while a haematin solution under the same conditions gives the spectrum of an alkaline haemochromogen solution (see below). Methaemoglobin in alkaline solution shows two absorption- bands which are like the two oxyhaemoglobin bands, but they differ from these in that the band ft is stronger than a. By the side of the band a and united with it by a shadow lies a third, fainter band between C and D, near to D. Crystallized methaemoglobin may be easily obtained by treating a concentrated solution of oxyhaemoglobin with a sufficient quanti- ty of concentrated potassium ferricyanide solution to give the mix- ture a porter-brown color. After cooling to 0° C. add £ vol. cooled alcohol and allow the mixture to stand a few days in the cold. The crystals may be easily purified by recrystallizing from water by the addition of alcohol. Carbon monoxide methaemoglobin has been prepared by WEYL and v. ANSEP by the action of potassium permanganate on carbon monoxide haemo- globin. Sulphur methaemoglobin is the same given by HOPPE-SEYLER to that coloring matter which is formed by the action of sulphuretted hydrogen on oxyhaemoglobin. ^ The solution has a greenish-red, dirty color and shows an absorption-band in the red. This coloring matter is claimed to be the greenish color seen on the surface of putrefying flesh. Decomposition products of the blood coloring matters. By its decomposition haemoglobin yields, as above stated, albumin and a ferruginous coloring matter as chief products. If the de- composition takes place in the absence of oxygen, a coloring matter 76 PHYSIOLOGICAL CHEMISTRY. is obtained which is called by HOPPE-SEYLER hwmochromogen, by other investigators (STOKES) reduced hoematin. In the presence of oxygen, haemochromogen is quickly oxidized to haematin, and we obtain in this case as a colored decomposition product another coloring matter, Jicematin. As haemochromogen is easily converted by oxygen into haematin, so this latter may be reconverted into haemochromogen by reducing substances. Hsemochromogen was discovered by HOPPE-SEYLER to whom of all investigators we are more indebted for our knowledge in regard to the blood-coloring matters and their decomposition products. He has also lately been able to obtain this coloring matter as crystals. Haemochromogen is, according to HOPPE-SEYLER, the colored atomic group of haemoglobin and its combination with gases, and this atomic group is combined with albumin in the coloring matter. The characteristic absorption of light depends on the haemochromogen, and it is also this atomic group which binds in the oxyhaemoglobin 1 mol. oxygen and in the carbon monoxide haemoglobin 1 mol. carbon monoxide with 1 atom iron. HOPPE- SEYLER has observed a combination between haemochromogen and carbon monoxide, and this combination shows the spectral appear- ance of carbon monoxide haemoglobin. An alkaline haemochromogen solution has a beautiful red color. It shows two absorption-bands, first described by STOKES, of which the one is darker and lies between D and E, and the other, broader but not so dark, covers the lines E and b. In acid solution haemo- chromogen shows four bands, which according to JADERHOLM de- pend on a mixture of haemochromogen and haematoporphyrin (see below), this last formed by a partial decomposition resulting from the action of the acid. Haemochromogen may be obtained as crystals by the action of caustic soda on haemoglobin at 100° C. in the absence of oxygen (HOPPE-SEYLER). By the decomposition of haemoglobin by acids (of course in the absence of air) we obtain haemochromogen con- taminated with a little haematoporphyrin. An alkaline haemochro- mogen solution is easily obtained by the action of a reducing sub- stance (STOKES* reduction liquid) in an alkaline haematin solution. Haematin, also called Oxyhaematin, is sometimes found in old transudations. It is formed by the action of gastric or pancreatic THE BLOOD. 77 juices on oxyhaemoglobin, and is therefore also found in the faeces after hemorrhage in the intestinal canal, and also after a meat diet and food rich in blood. It is stated that haematin may occur in urine after poisoning with arseniuretted hydrogen. As shown above, the haematin is formed by the decomposition of oxyhaemo- globin, or at least of haemoglobin, in the presence of oxygen. The constitution of haematin may, according to HOPPE-SEYLEK, be expressed by the formula C^H^N^FeOg. According to NENCKI and SIEBER it has the formula C32Hb2N4re04 , and they claim that - haematin consists of a body not yet isolated, haemin, C^HgoN^FeOs > with 1 mol. H20. Haematin is amorphous, dark brown or bluish black. It may be heated to 180° C. without decomposition; on burning it leaves a residue consisting of iron oxide. It is insoluble in water, dilute acids, alcohol, ether, and chloroform, but it dissolves slightly in warm glacial acetic acid. Haematin dissolves in acidified alcohol or ether. It easily dissolves in alkalies, even when very dilute. The alkaline solutions are dichroitic; in thick layers they appear red by reflected light, and in thin layers greenish. The alkaline solutions are precipitated by lime- and baryta-water, as also by solutions of neutral salts of the alkaline earths. The acid solutions are always brown. An acid haematin solution absorbs the red part of the spectrum less and the violet part more. The solution shows a rather sharply- defined band between C and D whose position may change with the variety of acid used as a solvent. Between D and F a second, much broader, less sharply-defined band occurs which by proper dilution of the liquid is converted into two bands. The one between b and Fy lying near F, is darker and broader, the other, between D and E, lying near E, is lighter and narrower. Also by proper dilution a fourth very faint band is observed between D and E lying near D. Haematin may thus in acid solution show four absorption-bands; ordinarily one sees distinctly only the bands between C and D and the broad, dark band— or the two bands— between D and F. In alkaline solution the haematin shows a broad absorption-band, which lies in greatest part between C and D, but reaches a little over the line D towards the right in the space between D and E. Haematin is dissolved by concentrated sulphuric acid in the 78 PHYSIOLOGICAL CHEMISTRY. presence of air, forming a purple-red liquid. The iron is here split off and the new coloring matter, called licematoporpliyrin by HOPPE-SEYLER, is iron-free. The hgematin yields with concentrat- ed sulphuric acid, in the absence of air, a second iron-free coloring matter called hcsmatolin (HOPPE-SEYLER). Haematoporphyrin may also be prepared by the action of glacial acetic acid saturated with hydrobromic acid on haemin crystals (NENCKI and SIEBER). This coloring matter is, according to NENCKI and SIEBER, an isomer of the bile-coloring matter bilirubin, and the formula is, according to them, C16H18N203. The formation of haematoporphyrin from haematin occurs according to the following equation : CaE.NAFe + 2H30 - Fe = 2(C16H18N303). The combinations of haematoporphyrin with Na and with HC1 have been obtained as crystals by NENCKI and SIEBER. The haematoporphyrin prepared by them does not seem to be identical with that prepared by HOPPE-SEYLER even though they have the same spectrum. A dilute solution with alkali carbonate shows a spectrum with four absorption-bands, namely, a band between 0 and D\ a second, broader, surrounding D and with its broadest part between D and E\ a third, lighter and narrower, between D and E\ and lastly a fourth, broad and dark band between b and F. By the action of reducing agents on haematoporphyrin a coloring matter has been obtained which stands closely related to urobi- lin (HOPPE-SEYLER, NENCKI and SIEBER). Haematoporphyrin occurs also in the animal kingdom preformed (MAcMu^K). Haemin, H^SMI^ CRYSTALS, or TEICHMANK'S CRYSTALS. Hae- min, according to HOPPE-SEYLER, is a combination between hae- matin and hydrochloric acid, having the formula C^Hggl^FeOs.HCl. NEKCKI and SIEBER designate as haemin, on the contrary (see page 77), a body not yet isolated, of the formula C32H30N4Fe03, which may be considered as haematin — H20 or C32H32N4Fe04 — H20. The haemin crystals are, according to the latest views, a combina- tion of this substance, haemin, and HC1, according to the formula According to the same experimenters,the hgemin crystals are a double com- bination with the solvent, amyl alcohol or acetic acid, which is used in their preparation; while HOPPE-SEYLEB claims that the solvent is only held me- THE BLOOD. 79 chanically by the crystals. The formula of the hsemin crystals prepared by means of amyl alcohol is, according to NENCKI and SIEBER, Haemin crystals form in large masses a bluish-black powder, but are so small individually that they can only be seen by the micro- scope, They consist of dark-brown or nearly brownish-black, long, rhombic, or spool-like crystals, isolated, or grouped as crosses, rosettes, or starry forms. They are insoluble in water, dilute acids at the normal temperature, alcohol, ether, and chloroform. They are slightly dissolved by glacial acetic acid and warmth. They dissolve in acidified alcohol, as also in dilute caustic or carbonated alkalies; and in the last case they form, besides alkali chlorides, soluble haematin alkali, from which the haematin may be precipi- tated by an acid. The preparation of haemin crystals is always the starting-point for the preparation of haematin. According to HOPPE-SEYLER, shake the blood-corpuscles which have been washed with common- salt solution (see page 66) with water and ether, then filter the solu- tion of blood-coloring matters, concentrate strongly, mix with 10-20 vols. glacial acetic acid, and heat for 1-2 hours on the water-bath. After diluting with several volumes of water, allow the liquid to stand a few days. The crystals which separate are then washed with water, boiled with acetic acid, and then washed again with water, alcohol, and ether. NENCKI and SIEBEK coagulate the sediment of the blood-corpuscles by alkali, allow the coagulum to dry incompletely in the air, rub it fine, and then boil it with amyl alcohol after the addition of a little hydrochloric acid. The crys- tals which separate from the filtrate after cooling are washed with water, alcohol, and ether. If haemin crystals be dissolved in dilute caustic alkali, haematin may be precipitated from the solution by the addition of acid; and from this haematin pure haemin crystals may be prepared by heating with glacial acetic acid and a little common salt. In preparing haemin crystals in small amounts proceed in the following manner : The blood is dried after the addition of a small quantity of common salt, or the dried blood may be rubbed with a trace of common salt. The dry powder is placed on a microscope- slide, moistened with glacial acetic acid, and then covered with the cover-glass. Add, by means of a glass rod, more glacial acetic acid by applying the drop at the edge of the cover-glass, until the space between the slide and the cover-glass is full. Now warm over a very small flame, with the precaution that the acetic acid does not boil 80 PHYSIOLOGICAL CHEMISTRY. and pass with the powder from under the cover-glass. If no crys- tals appear after the first warming and cooling, warm again, and if necessary add some more acetic acid. After cooling, if the experi- ment has been properly performed, a number of dark-brown or nearly black haemin crystals of varying forms will be seen. Haematoidin, thus called by VIRCHOW, is a coloring matter which crystallizes in orange-colored rhombic plates, and which occurs in old blood extravasations, and whose origin from the blood-coloring matters seems to be established (LANGHANS, CORDUA, QUINCKE, and others). A solution of haematoidin shows no absorption-bands, but only a strong absorption of the violet to the green (EWALD). According to most observers, haema- toidin is identical with the bile-coloring matter bilirubin. It is not identical with the crystallizable lutein from the corpora lutea of the ovaries of the cow ( PICCOLO and LIEBEN, KUHNE and EWALD). In the detection of the above-described blood-coloring matters the spectroscope is the only entirely trustworthy means of investi- gation. If it is only necessary to detect blood in general and not to determine definitely whether the coloring matter is haemoglobin, methaemoglobin, or haematin, then the presence of hasmin crystals is an absolute positive proof. The reader is referred to more extended text-books for exacter methods for the detection of blood in chemico-legal cases, and it is perhaps sufficient to give here the chief points of the investigation. If spots on clothes, linen, wood, etc., are to be tested for the pres- ence of blood, it is best, when possible, to scratch or shave off as much as possible, rub with common salt, and from this prepare the haemin crystals. On obtaining positive results the presence of blood is not to be doubted. If you do not obtain sufficient material by the above means, then soak the spot with a few drops of water in a watch-crystal. If a colored solution is thus obtained, then remove the fibres, wood-shavings, and the like as far as possible, and dry all the solution in a watch-glass. The dried residue may be partly used for the spectroscope test directly, and part may be employed in the preparation of the haemin crystals. It also serves to detect haemochromogen in alkaline solution after previous treat- ment with alkali and the addition of reducing substances. If a colorless solution is obtained after soaking with water, or the spots are on rusty iron, then digest with a little dilute alkali (5 p. m.). In the presence of blood the solution gives, after neu- tralization with hydrochloric acid and drying, a residue which may THE BLOOD. 81 give the haemin crystals with glacial acetic acid. Another part of the alkaline solution shows, after the addition of STOKE'S reduc- tion liquid, the absorption-bands of haemochromogen in alkaline solution. The methods proposed for the quantitative estimation of the blood-coloring matters are partly chemical and partly physical. Among the chemical methods to be mentioned is the ashing of the blood and the determination of the amount of iron contained therein, from which the amount of haemoglobin may be calculated. Another method consists in first saturating the blood completely with oxygen. Now pump out thor- oughly this oxygen, and calculate from the amount of oxygen the amount of haemoglobin present (GREHANT and QUINQUAUD). None of these methods is reliable. The physical methods consist either in a colorimetric or a spec- troscopic investigation. The principle of HOPPE-SEYLER'S colorimetric method is that a measured quantity of blood is diluted with an exactly measured quantity of water until the diluted blood solution has the same color as a pure oxyhaemoglobin solution of a known strength. The amount of coloring matter present in the undiluted blood may be easily calculated from the degree of dilution. In the colorimetric testing we use a glass vessel with parallel sides containing a layer of liquid 1 cm. thick (haematinometer of HOPPE-SEYLER). The method is good, and the inconvenience that the normal solution of oxyhaemoglobin does not keep for any length of time without de- composing may be prevented by preserving the solution in sealed tubes. The oxyhaamoglobin is gradually reduced to a haemoglobin solution which may be kept for years, and when required for use it is converted into an oxyhaemoglobin solution by aerating. A few observers (RAJEWSKY, LESSER" MALLASSEZ) have tried to replace the oxyhaemoglobin solution by a solution of picrocarmin. The quantitative estimation of the blood-coloring matters by means of the spectroscope may be done in different ways, but at the present time the spectrophotometric method is chiefly used, and this seems to be the most reliable. This method is based on the fact that the extinction coefficient of a colored liquid for a certain region of the spectrum is directly proportional to the concentra- tion, so that G : E = Ct : El , when C and Cl represent the differ- ent concentrations and E and E. the corresponding coefficient of C C extinction. From the equation ^ = —it follows that for one and Jii Jtl the same coloring matter this relation, which is called the absorp- tion ratio, must be constant. If the absorption ratio is represented by A, the determined extinction coefficient by E, and the concen- 82 PHYSIOLOGICAL CHEMISTRY. tration (the amount of coloring matter in grams in 1 c. c.) by C, then C = A.E. Different apparatus have been constructed (VIERORDT and HUFNER) for the determination of the extinction coefficient which is equal to the negative logarithm of those rays of light which remain after the passage of the light through a layer 1 cm. thick of an absorbing liquid. In regard to these apparatus the reader is referred to other text-books. As control the extinction coefficients are determined in two different regions of the spectrum, namely, D3&E—D51E and D§ZE—D%±E. The constants or the absorption ratio for these two regions of the spectrum are designated by HEFNER by A and A'. Before the determination the blood must be diluted with water, and if the proportion of dilution of the blood be represented by V, then the concentration or the amount of coloring matter in 100 parts of the undiluted blood is C= 100. V.A.E and 6'= 100. V.A'.E'. The absorption ratio or the constants in the two above-mentioned regions of the spectrum have been determined for oxyhaemoglobiu, haemoglobin, carbon monoxide haemoglobin, and methaemoglobiu. The figures for the above coloring matters obtained from canine blood are as follows : Oxy hemoglobin ............. A0 — 0.001330 and A0' — 0.001000 Hemoglobin ................. Ar = 0.001091 " Ar' = 0.001351 Carbon monoxide hemoglobin Ac — 0.001130 " Ae' = 0.001000 Methsemoglobin .............. Am— 0.003696 " Am'— 0.002798 The quantity of each coloring matter may be determined in a mixture of two blood-coloring matters by this method, which is of special importance in the determination of the quantity of oxyhaemoglobin and haemoglobin present in blood at the same time. If we represent by .Z^and E' the extinction co- efficients of the mixture in the above-mentioned regions of the spectrum, by AO and AO and Ar and Ar' the constants for oxyhaemoglobin and reduced haemoglobin, and by Fthe degree of dilution of the blood, then the percentage of oxyhaemoglobin H0 and of (reduced) haemoglobin Hr is and Hr = 100 V. Among the many apparatus constructed for clinical purposes for the quantitative estimation of haemoglobin the haemometer of FLEISCHL is to be preferred. The determination by this apparatus is made by comparing the color of the blood diluted with water with the color of a wedge-shaped movable prism of red glass. If the blood shows the same color as the glass prism, then the amount of haemoglobin in the blood may be directly read from the scale. THE BLOOD. 83 The amount of haemoglobin is expressed as percentage of the physiological amount of haemoglobin. Many other coloring matters are found besides the often-occurring haemo- globin in the blood of in vertebra. In a few arachnidae, Crustacea, gasteropodae, and cephalopodae a body analogous to haemoglobin containing copper, hcemo- cyanin, has been found. By the taking up of loosely-bound oxygen this body is converted into blue oxyhwmocyaiiin (P. BERT, FREDERICQ, KRUKENBERG, M.\cMuNN), and by the escape of the oxygen becomes colorless again. A coloring matter called chlorocruorin by LANKESTER is found in certain chaetopodae. Hwmerythrin, so called by KRUKENBERG but first observed by SCHWALBE, is a red coloring matter from a few gephyrea. Besides haemo- cyanin we find in the blood of certain Crustacea the red coloring matter tetronerythrin (HALLIBURTON), which is also widely spread in the animal kingdom. EcJiinochrom, so named by MAcMuNN, is a brown coloring matter occurring in the perivisceral fluid of a variety of echinoderms. The quantitative constitution of the red blood-corpuscles is difficult to determine and we have hardly any sufficiently trust- worthy analyses of them. The amount of water varies in different varieties of blood between 570-630 p. m., with a proportional amount, 430-370 p. m., of solids. In the blood of mammalia the chief mass (about nine tenths) of the dried substance consists of haemoglobin. According to the analyses of HOPPE-SEYLER and his pupils, the red corpuscles contain in 1000 parts of the dried substance: Human blood . . Dog " Goose " . . Snake " Haemoglobin. .. 868-943 .. 865 627 467 Albumin. 123-51 126 364 525 Lecithin. 7.2-3.5 6.0 5.0 Cholesterin. 2.5 4.0 .5.0 Of special interest is the varying proportion of the haemo- globin to the albumin in the nucleated and in the non-nucleated blood-corpuscles. These last are much richer in haemoglobin and poorer in albumin than the others. The amount of mineral bodies, as far as they have been determined, in the moist corpuscles is 4.8- 8.9 p. m. The chief mass consists of potassium, phosphoric acid, and chlorine. The blood-corpuscles of ox-blood contain, accord- ing to BUNGE, more sodium and chlorine than phosphoric acid and potassium. The blood-corpuscles of the pig and horse contain no sodium (BUNGE). Human-blood corpuscles contain, according to WANACH, about five times as much potassium as sodium, on an average 3.99 p. m. potassium and 0.75 p. m. sodium. 84 PHYSIOLOGICAL CHEMISTRY. The Colorless Blood-corpuscles and the Blood-tablets. The Colorless Blood - corpuscles, also called LEUCOCYTES or Lymphoid Cells, which occur in the blood in varying forms and sizes, form in a state of rest spherical lumps of a sticky, highly refractive power, capable of motion, non-membranous protoplasm, which show 1-4 nuclei on the addition of water or acetic acid. In human and mammalial blood they are larger than the red blood- corpuscles. They have also a lower specific gravity than the red corpuscles, move in the circulating blood nearer to the walls of the vessel, and have also a slower motion. The number of colorless blood-corpuscles varies not only in the different blood-vessels, but also under different physiological con- ditions. As an average we have only 1 colorless corpuscle for 350- 500 red corpuscles. According to the investigations of ALEX. SCHMIDT and his pupils, the leucocytes are destroyed in great part — about 71$ in the blood of the horse, according to HEYL — on the discharge of the blood before and during coagulation, so that dis- charged blood is much poorer in leucocytes than the circulating blood. The correctness of this statement has been denied by other investigators. From a histological standpoint we generally distinguish the different kinds of colorless blood-corpuscles; chemically considered, however, there is no known essential difference between them. With regard to their importance in the coagulation of fibrin ALEX. SCHMIDT and his pupils distinguish between the leucocytes which are destroyed by the coagulation and those which are not. The last mentioned are colored quickly by carmine and give with alkalies or common-salt solutions a slimy mass; the first do not show such behavior. The protoplasm of the leucocytes has during life amoeboid movements which partly make possible the wandering of the cells and partly the taking up of smaller grains or foreign bodies within the same. On these grounds the occurrence of myosin in them has been admitted even without any special proof thereof. ALEX. SCHMIDT found serum globulin in equine-blood leucocytes which had been washed with ice-cold water. There are also at least certain leucocytes which yield a slimy mass when treated THE BLOOD. 85 with alkalies or NaCl solutions, which seem to be identical with the so-called hyaline substance of ROVIDA found in the pus-cells. On digesting the leucocytes with water a solution of a proteid body is obtained which can be precipitated by acetic acid and which is not soluble in an excess of the acid (SCHMIDT and KAUSCHEN- BACH, WOOLDRIDGE). This proteid substance, designated " lymph - fibrinogen " by WOOLDRIDGE, when obtained from certain leucocytes (but not from others) has a powerful action on the coagulation of fibrin. An important constituent of the colorless corpuscles is, according to ALEX. SCHMIDT, the fibrin ferment or perhaps more correctly a mother-substance of the same, a zymogen. In the destruction of the colorless corpuscles the fibrin ferment is set free and serum globulin also passes into the plasma at the same time. HALLIBURTON has isolated two globulins and one albumin (see page 42) from the leucocytes of the lymphatic glands, besides Rovi- DA'S hyaline substance, and albumoses and peptones as post-mortem products. Among the other constituents of the pale corpuscles we must mention glycogen, which occurs in the living but not in the dead cell, lecithin, prof agon (?), and cholesterin. The nucleus consists without doubt of nuclein. The mineral constituents are probably the same as in the pus-cells (see Chapter V). The above statements in regard to the constituents of the leuco- cytes cover not only the pale blood-corpuscles, but also the leuco- cytes of the lymphatic glands. The leucocytes of the blood are considered as cells pierced from the outside, and it is probably the correct view because we know of no specific chemical properties or constituents in the leucocytes of the blood which differ from those of other leucocytes. The blood-tablets (BIZZOZERO'S), whose nature and physiological importance have been much questioned, are pale, colorless, gummy disks, round or more oval in shape and generally with a diameter two or three times smaller than the red blood-corpuscles. Their number, according to FUSARI, in healthy persons is 180,000-250,000 in 1 c.mm. Certain investigators, for example BIZZOZERO, SCHIMMELBUSCH, and LAKER, claim that the blood-tablets occur preformed in the circulating blood, while others, for instance LOWIT and WOOLDRIDGE, deny this. Other investigators (HLAVA) 86 PHYSIOLOGICAL CHEMISTRY. claim that they may, at least in part, be formed from the colorless blood-corpuscles, while LOWIT claims that they are formed by the withdrawal of globulin substance from the white blood-corpuscles. BIZZOZERO and several other investigators consider the blood -tablets as the starting-point for the coagulation of the blood, while ALEX. SCHMIDT'S pupils deny this. LOWIT claims that the blood-tablets are formed from globulin substance, and has therefore given them the name globulin tablets. The relationship of these blood-tablets to the fibrin coagulation will be spoken of shortly. III. The Blood as a Mixture of Plasma and Blood- corpuscles. The blood in itself is a thick, sticky, lighter or darker red opaque liquid having a salty taste and even in thin layers a faint odor dif- fering in different kinds of animals. On the addition of sulphuric acid to the blood the odor is more pronounced. In adult human beings the specific gravity averages 1.055, ranging between 1.045 and 1.075. According to SCHERREKZISS the foetal blood has a lower specific gravity than the blood of grown persons. LLOYD E. JOKES found that the specific gravity is highest at birth and lowest in children when about two years old and in pregnant women. The reaction of the blood is alkaline. The amount of alkali, calculated as Na2C03, is in the dog about 2 (ZUNTZ), in rabbits about 2.5 (LASSAR), and in man 3.38-3.90 p. m. (v. JAKSCH). The alkaline reaction diminishes outside of the body, and indeed the more quickly the greater the original alkalinity of the blood. This depends on the formation of acid in the blood, in which the red blood-corpuscles seem to take part in some way or another. After excessive muscular activity the alkalinity is diminished on account of the formation of acid in the muscles, and it is also decreased after the continuous use of acids (LASSAR). The color of the blood is red — light scarlet-red in the arteries and dark bluish-red in the veins. Blood free from oxygen is dichroitic, dark red by reflected light, and green by transmitted light. The blood-coloring matters occur in the blood-corpuscles. For this reason blood is opaque in thin layers and acts as a " deckf arbe." If the haemoglobin is removed from the stroma or dissolved from THE BLOOD. 87 the blood-liquid by any of the above-mentioned means (see page 66) the blood becomes transparent and acts then like a "lacfarbe." Less light is now reflected from its interior, and this latter blood is therefore darker in thicker layers. On the addition of salt solutions to the blood-corpuscles they shrink and more light is reflected and the color appears lighter. A great abundance of red corpuscles makes the blood darker, while by diluting with serum or by a greater abundance of white corpuscles the blood becomes lighter in appearance. The different colors of arterial and of ven- ous blood depend on the varying quantity of gas contained in these two varieties of blood, or better on the different amounts of oxy- haemoglobin and haemoglobin they contain. The reason for the different colors of these two varieties of blood has been attributed in part to the unequal forms of the blood-corpuscles. In the arterial blood the blood-corpuscles are considered as more biconcave and therefore reflect the light more than in the venous blood (HAKLESS). These statements do not coincide with later investigations. The most striking property of blood consists in its coagulating within a shorter or longer time, but as a rule very shortly after leaving the vein. Different kinds of blood coagulate with vary- ing rapidity ; in human blood the first marked sign of coagulation is seen in 2-3 minutes, and within 7-8 minutes the blood is thor- oughly converted into a gelatinous mass. If the blood is allowed to coagulate slowly, the red corpuscles have time to settle more or less before the coagulation, and the blood-clot then shows an upper, large, yellowish-gray or reddish -gray layer consisting of fibrin en- closing chiefly colorless corpuscles. This layer has been called crusta inflammatoria or phlogistica, because it has been especially observed in inflammatory processes, and is considered one of the characteristics of them. This crusta or "buffy coat" is not char- acteristic of any special disease, and it occurs chiefly when the blood coagulates slowly or when the blood-corpuscles settle more quickly than usual. A buffy coat has been observed often in the slow coagulation of equine blood. The blood from the capillaries is not supposed to have the power of coagulating. Coagulation is retarded by cooling, by diminishing the oxygen and increasing the amount of carbon dioxide, which is the reason that venous blood and to a much higher degree blood after asphyx- 88 PHYSIOLOGICAL CHEMISTRY. iation coagulates more slowly than arterial blood. The coagulation may be retarded or prevented by the addition of acids, alkalies, or ammonia, even in small quantities ; by concentrated solutions of neutral salts or alkalies and alkaline earths ; also by egg-albumin, solutions of sugar or gum, glycerin, or much water; also by receiv- ing the blood in oil. The coagulation may be prevented by the in- jection of an albumose solution or by an infusion of the leech into the circulating blood, but this infusion of the leech acts in the same way on blood just expelled. The coagulation may be facil- itated by raising the temperature; by contact with foreign bodies, to which the blood adheres ; by stirring or beating it ; by admission of air; by diluting with very small amounts of water; by the ad- dition of platinum-black or finely-powdered carbon ; by the addition of lac-colored blood, which does not act by the presence of dissolved blood-coloring matters, but by the stromata of the blood-corpuscles (WooLDRiDGE, Nucif), and also by the addition of the leucocytes from the lymphatic glands, or a watery saline extract of the lymph- atic glands, testicles, or thymus. The active constituent of such a watery extract is, according to WOOLDRIDGE, an albuminous body containing lecithin, which he calls tissue fibrinog en. An important question to answer is why the blood remains fluid in the circulation while it quickly coagulates when it leaves the circulation. When the blood leaves the vein it comes under new, abnormal conditions. It cools off, it comes in contact with the air, its motion stops, and it is deprived of the influence of the living walls of the vessels. That the cooling is not the reason of the coagulation is proved by the fact that cooling is a good means of retarding coagulation. That the contact with air is not essential is shown by the fact that when blood is collected over mercury, so that it cannot absorb or expel any gas, it likewise coagulates. That the cessation of the motion does not cause the coagulation follows, since blood collected over mercury coagulates whether it is shaken or not, and further from the fact that motion, such as beating the blood, facilitates the coagulation. The reason why blood coagulates on leaving the body is there- fore to be sought for in the influence which the walls of the living THE BLOOD. 89 and entire blood-vessels exert upon it. These views are derived from the observations of many investigators. From the observa- tions of HEWSON, LISTER, and FREDERICQ it is known that when a vein full of blood is tied at the two ends and removed from the body, the blood may remain fluid for a long time. BRUCKE allowed the heart removed from a tortoise to beat at 0°C., and found that the blood remained uncoagulated for some days. The blood from another heart quickly coagulated when collected over mercury. In a dead heart, as also in a dead blood-vessel, the blood soon coagu- lates, and also when the walls of the vessel are changed by pathological processes. What then is the influence which the walls of the vessels exert on the liquidity of the circulating blood ? FREUND has found that the blood remains fluid when collected by means of a greased canula under oil or in a vessel smeared with vaseline. If the blood collected in a greased vessel be beaten with a glass rod previously oiled, it does not coagulate, but it quickly coagulates on beating it with an unoiled glass rod or when it is poured into a vessel not greased. The non-coagulability of blood collected under oil has been confirmed later by HAYCRAFT and CARLIER. FREUND found on further investigating that the evaporation of the upper layers of blood or their contamination with small quantities of dust causes a coagulation even in a vessel treated with vaseline. According to FREUND, it is this adhesion between the blood or between its form-elements and a foreign substance — and the diseased walls of the vessels also act as such — that gives the impulse towards coagulation, while the lack of adhesion prevents the blood from coagulating. This adhesion of the form-elements of the blood to certain foreign substances seems to induce changes which apparently stand in a certain relationship to the coagulation of the blood. That view of the coagulation of the blood which, with a few modifications, is accepted by most investigators is the theory pro- posed by ALEXANDER SCHMIDT and the DORP AT SCHOOL. Accord- ing to ALEX. SCHMIDT, who of all investigators has done most to elucidate this subject, an abundant destruction of the colorless blood- corpuscles takes place in coagulation, and from this not only the fibrin ferment results, but also serum globulin and fibrinogen, which 90 PHYSIOLOGICAL CHEMISTRY. last, however, was previously present in the plasma. Under the action of the fibrin ferment the serum globulin and fibrinogen unite forming fibrin. In the coagulation a reciprocal action takes place between the protoplasm of the leucocytes and the plasma, so that the plasma quickly destroys the leucocytes and is itself destroyed by the setting free of fibrin ferment with the separation of fibrin (SCHMIDT and RAUSCHE^BACH). Such an exchange action not only takes place between the blood-plasma and the leucocytes, but also between blood-plasma and animal protoplasm in general — indeed, even between vegetable protoplasm and blood-plasma (SCHMIDT and GROHMANN). While the blood-serum in general acts as a conser- vator on the cells, the blood-plasma on the contrary has a destruc- tive action, and this action develops the fibrin ferment. This last- mentioned is chiefly a decomposition product of the cells (SCHMIDT, RAUSCHENBACH; FOA and PELLACAKI), and it may therefore be called " protozym" (RAUSCHENBACH). A destruction of the leuco- cytes may also occur in the blood under physiological conditions, and therefore traces of fibrin ferment are habitually forme'd in the blood. Within certain limits the organism may be guarded from a danger- ous increase in these processes. According to SCHMIDT and GROTH, and SCHMIDT and KRUGER, the injection of leucocytes into the circu- lating blood may produce an intravascular coagulation, but the cor- rectness of this statement is denied by WOOLDRIDGE. According to him, the pure, washed leucocytes are inactive and the action ob- served by SCHMIDT and his pupils is caused by contamination with " lymphfibrinogen" The lymphfibrinogen is a representative of an entire group of protein substances which are precipitated by acetic acid, contain lecithin, which occur in many organs and tissues but which have not been closely studied and which WOOLDRIDGE has named " tissue fibrinogen s." According to ALEX. SCHMIDT, the coagulation of the blood is an enzymotic process, produced by the fibrin ferment, in which two protein substances, the serum globulin and the fibrinogen, form the material substrata of the fibrin. There is no basis for the assump- tion that the serum globulin in the newly-formed fibrin exists otherwise than as a mechanical contamination. It is true indeed that the strongly-contaminated serum globulin prepared from serum containing enzymes accelerates coagulation, and the amount THE BLOOD. 91 of separated fibrin under the circumstances may be increased, but pure serum globulin prepared from transudation free from enzymes is inactive. The same action on the amount of fibrin separated which the impure serum globulin shows also occurs by the action of the substance precipitated by acetic acid from a watery extract of the leucocytes of the lymphatic glands. This substance, which is neither identical with serum globulin nor contaminated by it, may, according to SCHMIDT and RAUSCHENBACH, increase the amount of fibrin in filtered plasma about 25$. Finally, a fibrinogen solution free from serum globulin, and a fibrin ferment solution also free from serum globulin, may yield a typical fibrin (AUTHOR). It is therefore hardly possible to accept this part of SCHMIDT'S theory. It is much more probable that the impure serum globulin furthers the separation of the fibrin indirectly, in the same way as CaCl2 (AUTHOR) and the lime salts in general (GREEN, KRUGER). It is a fact that a typical fibrin is formed from fibrinogen alone in the presence of fibrin ferment and mineral bodies (alkali chlorides and lime salts). In regard to the importance of the colorless blood-corpuscles in the coagulation of the blood opinions are somewhat diverse. Ac- cording to BIZZOZERO and others, it is not the colorless blood- corpuscles but the blood-tablets which represent the starting-point for the formation of fibrin, a view against which weighty objec- tions have been urged by LOWIT and others. WOOLDRIDGE also considers the colorless blood-corpuscles as only of secondary im- portance. As found by him, a peptone-plasma which has been freed from all form-constituents by centrifugal force yields large quantities of fibrin when it is not separated from a substance which precipitates by cooling and which, on microscopical exami- nation, is very similar to BIZZOZERO'S blood-tablets. Neverthe- less, as LOWIT has found that homogeneous drops may exude from the white blood-corpuscles before the coagulation which take a form similar to tablets, it is probable that the substance observed by WOOLDRIDGE which, on cooling, separates as a formation similar to tablets originates from the colorless blood-corpuscles. That a coagulation without a destruction of the colorless blood- corpuscles may take place has been clearly demonstrated by LOWIT, who, however, does not dispute the importance of the 92 PHYSIOLOGICAL CHEMISTRY. colorless blood-corpuscles in the coagulation. On the contrary, he has observed that in crab's blood a so-called " plasmoschise" which is the exit of constituents of the cells in the plasma, takes place before the coagulation, and this process, he claims, stands in close relationship to the coagulation of the blood. If we do not give too much weight to the destruction of the leucocytes, — and we admit as most essential this part of SCHMIDT'S theory that the impulse to the coagulation comes from the colorless blood-corpuscles, and that the constituents of the same, passing into the plasma, take part in the coagulation, — then SCHMIDT'S theory is not disproved by the investigations of the last years, but it is even supported by them. In opposition to the view of ALEX. SCHMIDT, who considers the fibrin coagulation as an enzymotic process, WOOLDRIDGE is of the opinion that the fibrin ferment is not the cause of the coagu- lation, but is a product of the chemical processes taking place during coagulation. WOOLDRIDGE claims, on the contrary, that lecithin and proteid substances containing lecithin are of the greatest importance in the coagulation. This product is obtained by cooling the peptone-plasma which has been centrifugated, and the substance which separates has been called by WOOLDRIDGE ./4-fibrinogen. The plasma, according to WOOLDRIDGE, contains in itself all qualities necessary to produce a coagulation, and the form-elements are only of a secondary importance. Peptone-plasma which has been centrifugated and which is entirely free from form- elements, but contains the ^4-fibrinogen, coagulates on diluting with water, by the passage of carbon dioxide through the liquid, or after the addition of a little acetic acid, and the fibrin ferment is thereby formed. WOOLDRIDGE designates as 6-fibrinogen the ordi- nary fibrinogen isolated by the method suggested on page 56. This fibrinogen occurs indeed in transudations, but it only occurs in the peptone-plasma in very small quantities. A third fibrinogen occurs in the greatest amounts in the peptone-plasma, and this is the mother-substance of the (7-fibrinogen, and called i?-fibrinogen by WOOLDRIDGE. The J9-fibrinogen is converted into fibrin by lecithin and leucocytes from the lymphatic glands, but not by fibrin ferment or blood-serum. After the previous action of serum or fibrin ferment the ^-fibrinogen yields fibrin on diluting with water. The one most essential for the fibrin coagulation is, ac- THE BLOOD. 93 cording to WOOLDRIDGE, a reciprocal action between A- and B- fibrinogen. An exchange of lecithin from the .4-fibrinogen to the ^-fibrinogen takes place. HALLIBURTON has opposed weighty arguments to this theory. It is also difficult to find in WOOLDRIDGE'S discussion conclusive proofs for the above views, and the experiments by which they are supported are interpreted with difficulty. The entire theory is chiefly based on experiments with "peptone-plasma;" but such plasma acts differently in certain respects than ordinary plasma. It should be especially mentioned that, while ordinary plasma and an ordinary fibrinogen solution act similarly on heating, the pep- tone-plasma shows quite different behavior. The peptone-plasma when diluted with water, or on passing C02 through it, or on the ad- dition of a little acid, also acts, when the so-called ^4-fibrinogen has not been removed, like a plasma in which the ordinary fibrinogen ((7-fibrinogen) has been partly converted into an intermediate step between fibrinogen and fibrin. Until the differences existing be- tween ordinary plasma and peptone-plasma shall have been more thoroughly tested and generally accepted it will be difficult to give an unambiguous interpretation of observations made on peptone- plasma. FREUND seeks the reason for the coagulation in a separation of calcium phosphate whereby a part ,of the previously-dissolved albuminous bodies becomes insoluble as fibrin. By the adhesion existing before the coagulation the alkali phosphate of the form- elements passes over to the plasma richer in lime salts and forms calcium phosphate. If the amount of this last is so large in the plasma or any other coagulable liquid that it cannot be kept com- pletely in solution, then, according to FREUND'S views, the separa- tion of the excess is the cause of a part of the albuminous bodies becoming insoluble, or, in other words, is the cause of the coagula- tion. It is a well-known -fact that fibrin and fibrinogen yield an ash containing calcium phosphate, and that lime salts facilitate coagulation or cause a coagulation in liquids poor in ferment; and it is also well known that rennet ferment cannot cause a coagula- tion in a casein solution if there is a lack of lime salts. There is no question about the lime salts being of great importance in the fibrin coagulation ; but that a separation of calcium phosphate is 94 PHYSIOLOGICAL CHEMISTRY. the cause of coagulation has been just as little proved as the asser- tion that the coagulation of milk in the preparation of cheese is produced by the calcium phosphate becoming insoluble without the action of the rennet ferment on the casein. The untenability of FREUND'S theory has been shown by LATSCHENBERGER. According to DOGIEL and HOLZMANN, the coagulation of the fibrin is an oxidation of the fibrinogen. The relation of oxygen to the coagulation is not quite clear; a certain influence, however, on the coagulation cannot be denied. But as the coagulation may take place also in the absence of free oxygen, the above statement seems not to be well grounded. The very dark and complicated process of the intravascular coagulation and the relationship of the so-called tissue fibrinogen (WOOLDRIDGE) to the same have been so insufficiently studied that we cannot enter here into a discussion of this interesting question. IV. The Gases of the Blood. Since the pioneer investigations of MAGNUS and LOTHAR MEYER the gases of the blood have been subjects for repeated, care- ful investigations by prominent experimenters, among whom we must mention first C. LUDWIG and his pupils and E. PFLUGER and his school. By these investigations not only has science been enriched by a mass of facts, but also the methods themselves have been made more perfect and accurate. In regard to these methods, as also in regard to the laws of the absorption of gases by liquids, dissociation, and other questions belonging here, the reader is referred to complete text-books on physiology, on physics, and on gasometric analysis. The gases occurring in blood under physiological conditions are oxygen, carbon dioxide, and nitrogen. The last-mentioned gas is only found in very small amounts, on an average of 1.8 vol. per cent. The amount is here, as in all following experiments, calculated for 0° C. and 760 mm. pressure. The nitrogen seems to be simply ab- sorbed into the blood, at least in great part. It appears to play no part in the processes of life, and its amount varies but slightly in the blood of different blood-vessels. The oxygen and carbon dioxide behave otherwise, as their THE BLOOD. 95 amounts have significant variations, not only in the blood from different blood-vessels, but also because many conditions, such as a difference in the circulation, a different temperature, rest and ac- tivity, cause a change. The arterial and venous blood show the greatest difference in regard to the gases they contain. The amount of oxygen in the arterial blood of dogs is on an average 22 vols. per cent (PFLUGER). In human blood SETSCHENOW found about the same amount, namely, 21.6 vols. per cent. Lower figures have been found for rabbit's or bird's blood, respectively 13.2$ and 10-15$ (WALTER, JOLYET). Venous blood has very vari- able amounts of oxygen. LUDWIG and SCZELKOW found 6.8$ oxy- gen in the venous blood of quiet muscles and a still smaller amount in the venous blood of active muscles. Oxygen is entirely absent from blood after asphyxiation, or only occurs as traces. The venous blood of the glands seems, on the contrary, during secretion to be richer in oxygen than ordinary venous blood. By summarizing a great number of analyses by different experimenters, ZUNTZ has calculated that the venous blood of the right side of the heart con- tains on an average 7.15$ less oxygen than the arterial blood. The amount of carbon dioxide in the arterial blood of dogs is 30 to 40 vols. per cent (LUDWIG, SETSCHENOW, PFLUGER, P. BERT, and others), most generally about 40$. SETSCHENOW found 40.3 vols. per cent in human arterial blood. The amount of carbon dioxide in venous blood varies still more (LUDWIG, PFLUGER and their pupils, P. BERT, MATHIEU and URBAHST, and others). Accord- ing to the calculations of ZUNTZ the venous blood of the right side of the heart contains about 8.2$ more carbon dioxide than the arterial. The average amount may be put down as 48 vols. per cent. HOLMGREN found indeed in blood after asphyxiation 69.21 vols. per cent carbon dioxide. Oxygen is absorbed only to a small extent by the plasma or serum, in which PFLUGER found but 0.26$. The greater part or nearly all of the oxygen is loosely combined with the haemo- globin in oxyhaemoglobin. The quantity of oxygen which is con- tained in the blood of the dog corresponds closely to the quantity which we would expect from the activity of the haemoglobin to combine with oxygen, and also the quantity of haemoglobin in Canine blood. It is difficult to ascertain how far the circulating 96 PHYSIOLOGICAL CHEMISTRY. arterial blood is saturated with oxygen, as immediately after bleed- ing a loss of oxygen always takes place. Still it seems to be un- questionable that it is not quite completely saturated with oxygen in life. Arterial canine blood, according to PFLUGER, is saturated to T9^ with oxygen, according to HUFKER to -j-|. The question whether ozone occurs in the blood is to be answered decidedly in the negative. It is not only impossible to detect ozone in the blood, but the possibility of the occurrence of ozone in the fluids and tissues is even a priori to be denied. Ozone acts as nascent oxygen; and as easily-oxidized substances occur in the organism which combine with nascent oxygen, ozone, if such a formation should take place at all, would be destroyed instantly. But such a formation of ozone in the animal body cannot be admitted. Ozone may indeed be formed by slow oxidation, since the nascent oxygen formed in consequence combines with neutral oxygen forming ozone; but in the animal organism the nascent oxygen must be bound by the oxidized substances before it can form ozone. It was formerly believed that the haemoglobin acted as an " ozone-exciter," possessing the property of converting the inactive oxygen of the air into ozone. The red blood-corpuscles can by themselves also give a blue color with tincture of guaiacum, which is markedly seen when this tincture is dried on blotting-paper and a drop of blood previously diluted with 5-10 vols. water is added. According to PFLUGER, we are here dealing with a decomposition and gradual oxidation of haemoglobin, in which processes the neu- tral oxygen is split, setting free oxygen atoms. The carbon dioxide of the blood occurs in part, and indeed, ac- cording to the investigations of ALEX. SCHMIDT and L. FREDERICQ, generally £ in the blood- corpuscles, and it also occurs in part, and in fact the greatest part, in the plasma and serum respectively. Of the carbon dioxide in the form-elements a small part, according to SETSCHENOW, occurs in the colorless corpuscles (probably combined with the globulin alkali), while the chief mass exists in the red blood-corpuscles. The carbon dioxide of the red corpuscles is loosely combined, and the constituent uniting with the C02 of the same seems to be the alkali combined with phosphoric acid, oxyhaemoglobin or haemoglobin and globulin on one side and the haemoglobin itself on the other. THE BLOOD. 97 That in the red corpuscles alkali phosphate occurs in such quanti- ties that it may be of importance in the combination with carbon dioxide is not to be doubted, and we must admit that from the diphosphate, by a greater partial pressure of the carbon dioxide, monophosphate and alkali carbonate are formed,while by a lower par- tial pressure of the carbon dioxide the mass action of the phosphoric acid comes again into play, so that with the carbon dioxide becom- ing free, a re-formation of alkali diphosphate takes place. It is generally admitted that the blood-coloring matters, especially the oxy haemoglobin, which can expel carbon dioxide from sodium car- bonates in vacuo (PREYER), act like an acid; and as the globulin also acts like an acid (see below), this body may also occur in the blood- corpuscles as alkali combination. The alkali of the blood-corpuscles must therefore, according to the law of the action of the mass be- tween the carbon dioxide, phosphoric acid, and the others, be con- sidered as active acid constituents of the blood-corpuscles, and among these especially the blood-coloring matters, as the globulin can hardly be of importance because of its small quantity. By greater mass-action or greater partial pressure of the carbon dioxide, bicar- bonate must be formed at the expense of the diphosphates and the other alkali combinations, while at a diminished partial pressure of the same gas, with the escape of carbon dioxide, the alkali diphos- phate and the other alkali combinations must be re-formed at the cost of the bicarbonate. Haemoglobin must nevertheless, as the investigations of SET- SCHENOW and ZUNTZ and especially those of BOHR and TORUP have shown, be able to hold the carbon dioxide loosely combined even in the absence of alkali. BOHR has also found that the dis- sociation curve of the carbon-dioxide haemoglobin corresponds essentially to the curve of the absorption of carbon-dioxide, from which ground he and TORUP consider the haemoglobin itself as of importance in the binding of the carbon dioxide of the blood and not its alkali combinations. In regard to this question the conditions are not quite clear. If carbon dioxide is allowed to act on haemoglobin, it unites (BoHR, TORUP) with the colored atomic- group of the haemoglobin, splitting off albumin, and from this haemoglpbin, so decomposed, oxyhsemoglobin cannot be formed by the action of oxygen. According to BOHR, for each gramme of 98 PHYSIOLOGICAL CHEMISTRY. haemoglobin at -j- 18.4° C. and a pressure of 30 mm. 2.4 c. cm. carbon dioxide are combined ; and since in the arterial blood nearly all the haemoglobin exists as oxyhaemoglobin, it is difficult to obtain, at least from the arterial blood, an appreciable fraction of the carbon dioxide as carbon-dioxide haemoglobin. It cannot be denied that a not insignificant part of the carbon dioxide of the blood is held by the red corpuscles in loose combination ; but how this combination takes place requires further investigation before it can be answered. The chief part of the carbon dioxide of the blood is found in the blood-plasma or the blood-serum,, which follows from the fact that the serum is richer in carbon dioxide than the correspond- ing blood itself. By experiments with the air-pump on blood- serum it has been found that the chief part of the carbon dioxide contained in the serum is given off in a vacuum, while a smaller part can only be pumped out after the addition of an acid. The red corpuscles also act as an acid, and therefore in blood all the carbon dioxide is expelled in vacua. A part of the carbon dioxide is firmly chemically combined in the serum. Absorption experiments with blood-serum have shown us further that the carbon dioxide which can be pumped out is in great part loosely chemically combined, and from this loose combi- nation of the carbon dioxide it necessarily follows that the serum must also contain simply-absorbed carbon dioxide. For the form of binding of the carbon dioxide contained in the serum or the plasma we find the three following possibilities : 1. A part of the carbon dioxide is simply absorbed; 2. Another part is loosely chemically combined; and 3. A third part is in firm chemical combination. The quantity of simply-absorbed carbon dioxide has not been exactly determined. SETSCHEKOW considers the quantity in dog- serum to be about -fa of the total quantity of carbon dioxide. According to the tension of the carbon dioxide in the blood and its absorption coefficient, the quantity seems to be still smaller. The quantity of firmly chemically combined carbon dioxide in the blood-serum is the same as the quantity of simple alkali carbon- ate in the serum. This quantity is not known, and it cannot be determined either by the alkalinity found by titration, nor can it be calculated from the excess of alkali found in the ash, because the TJ1IY1RSITT THE BLOOD. alkali is not only combined with carbon dioxide but also with other bodies, especially with albumin. The quantity of firmly chemically combined carbon dioxide cannot be ascertained after pumping out in vacuo without the addition of acid, because to all appearances certain active constituents of the serum, as acids, expel carbon dioxide from the simple carbonate. The quantity of carbon dioxide not expelled from dog-serum by vacuum alone without the addition of acid amounts to 4.9 to 9.3 vols. per cent, according to the determinations of PFLUGER. From the occurrence of simple alkali carbonates in the blood- serum it naturally follows that a part of the loosely-combined carbon dioxide of the serum which can be pumped out must occur as bicarbonate. The occurrence of this combination in the blood- serum has been directly shown. In experiments with the pump, as well as in absorption experiments, the serum behaves in other ways as a solution of bicarbonate, or carbonate of a corresponding concentration; and the behavior of the loosely-combined carbon dioxide in the serum can be explained only by the occurrence of bicarbonate in the serum. By means of vacuum the serum always allows much more than one half of the carbon dioxide to be expelled, and it follows from this that in the pumping out not only may a dissociation of the bicarbonate take place, but also a conversion of the double sodium carbonate into a simple salt. As we know of no other carbon-dioxide combination besides the bicarbonate in the serum from which the carbon dioxide can be set free by simple dis- sociation in vacuo, we are obliged to assume that the serum must contain other faint acids in addition to the carbon dioxide, which contend with it for the alkalies and which expel the carbon dioxide from simple carbonates in vacuo. The carbon dioxide which is expelled by means of the pump and which, without regard to the simple absorbed quantity, is generally designated as " loosely chemi- cally combined carbon dioxide," is thus only obtained in part in dissociable loose combination; in part it originates from the sim- ple carbonates, from which it is expelled in vacuo by other faint acids. These faint acids are thought to be in part phosphoric acid and in part globulin. The importance of the alkali phosphates for the carbon dioxide combination (see page 97) has been shown by the 100 PHYSIOLOGICAL CHEMISTRY. investigations of FERNET; but the quantity of these salts in the serum is, at least in certain kinds of blood, for example in ox- serum, so small that it can hardly be of importance. In regard to the globulins SETSCHENOW is of the opinion that they do not act as acids themselves, but form a combination with carbon dioxide, producing carboglobulinic acid which unites with the alkali. Ac- cording to SERTOLI, whose views have lately found a supporter in TORUP, the globulins themselves are the acids combined with the alkali of the blood-serum. In both cases the globulin would form, directly or indirectly, that chief constituent of the plasma or of the blood-serum which, according to the law of the action of masses, contends with the carbon dioxide for the alkalies. By a greater partial pressure of the carbon dioxide the latter deprives the globulin alkali of a part of its alkali, and bicarbonate is formed ; by low partial pressure the carbon dioxide escapes, and the bicar- bonate is taken up by the globulin alkali. In the above statements it has been emphasized that the oxygen in the blood occurs in a dissociable combination with the haemo- globin, and for the formation of this combination, the oxyhsemo- globin, a certain partial pressure of the oxygen is necessary at any temperature. Also the carbon dioxide of the blood, that which is contained in the blood- corpuscles as well as that in the plasma, oc- curs mostly in combinations which are dependent to a great extent upon the partial pressure of the carbon dioxide. For the study of the exchange of gases between the blood and the alveolar air on one side, and the blood and the tissues on the other, special regard must be paid to the question as to how far this exchange of gases is the result of the law of diffusion and how far other forces take part in it; also the tension of the oxygen and the carbon dioxide is of the greatest importance. The law of the dissociation of oxyhaemoglobin has been studied by many investigators. Of the greatest physiological interest are those investigations which relate to the dissociation at the temper- ature of the body. In regard to these, many investigators (P. BERT, HERTER, and HUFNER) have found, partly by experiments on the living animal and partly by experiments with blood or pure haemo- globin solutions, that the tension of the oxygen of the blood at the temperature of the body corresponds to an oxygen partial pressure THE BLOOD. 101 of about 75-80 mm. mercury. BOHR has obtained a somewhat differ- ent result from his investigations. He experimented on dogs which were injected with leech infusion or peptone solution so as to prevent coagulation of the blood, and he allowed the blood to circulate through an apparatus which was inserted between the central and peripheral end of the cut carotid, or between the cen- tral end of the carotid and the central end of the cu t jugular vein, and in this way the exchange of gases between the blood and a gas mixture of known constitution could be determined. As measure of the tension of the oxygen in the arterial blood he obtained a dis- proportionate high value or an average pressure of 136.5 mm. mer- cury. By a simultaneous determination of the oxygen tension in the blood and in the expired air of the same animals he found in several cases a higher value for the former than for the latter. While according to P. BERT'S investigations the taking up of oxy- gen from the air of the lungs by the blood may be explained by the high oxygen partial pressure in the air of the lungs, this is not per- ceptible according to the experiments of BOHR, in which the ten- sion of the oxygen in the blood is greater than in the expired air, and also for evident reasons it is still greater than in the alveolar air. BOHR is also of the opinion that the generally-accepted diffu- sion theory does not give sufficient explanation for the taking up of oxygen from the air, and that we must also admit that the lung- tissue itself plays an active part in the taking up of oxygen. As the chief quantity of the oxygen in the blood simply ab- sorbed, corresponding to the pressure, is contained as a loose chem- ical combination, it is to be supposed that the amount of oxygen in the blood, at least within certain limits, is independent of the amount of oxygen in the air. That the raising of the oxygen pressure, even to a pressure of one atmosphere, has no essential influence on the amount of oxygen taken up, and on the carbon dioxide eliminated, has been known for a long time (LAVOISIER, EEGNAULT, and EEISET). Further experiments in this direction have been made by PAUL BERT. He found that in pure oxygen at a pressure of 3 atmospheres, or in or- dinary air at a pressure of 15 atmospheres, animals quickly died with convulsions. Before and during the spasms a lowering of temperature took place, and the consumption of oxygen, as well as 102 PHYSIOLOGICAL CHEMISTRY. the elimination of carbon dioxide and the combustion of the sugar of the blood, was lowered. By raising the oxygen pressure of the air to 3 atmospheres the amount of oxygen contained in the blood is somewhat increased. It seems that the amount of oxygen which is here taken up corresponds to that quantity which is simply ab- sorbed by the blood at that pressure. It is also of special interest to know to what extent the partial pressure of the oxygen of the air can be lowered without causing any injurious action or danger to life. A great many observations have been made on this subject, partly on man (P. BEET, SIVEL and CROCE-SPINELLI, LEBLAK c, and others) and partly on animals (by W. MULLER, HOPPE-SEYLER, STROGANOW, BERT, FRIEDLANDER and HERTER, FRANKEL and GEPPERT). From these observations it seems that the partial pressure of the oxygen in the atmosphere may sink to one half without causing a disturbance. The respira- tion is hindered by an oxygen tension of 7-8$ of an atmosphere, and at a still lower tension a lowering of the temperature is ob- served, lassitude, inability of muscular movement, and insensibility. At an oxygen tension which corresponds to about 3-3.5# of an atmosphere, death occurs. In regard to the amount of oxygen in the blood at lower air- pressures, the observations of FRANKEL and GEPPERT on dogs must be mentioned. At an air-pressure of 410 mm. mercury the amount of oxygen in the arterial blood was normal; at an air-pressure of 378-365 mm. it was a little diminished, and only at a lowering of the pressure'to 300 mm. was a marked decrease of the oxygen observed. The tension of the carbon dioxide in the blood has been deter- mined in different ways by PFLUGER and his pupils, WOLFBERG, STRASSBURG, and NUSSBAUM. According to the density method, the blood is allowed to flow directly from the artery or vein through a glass tube which contains a gas mixture of a known constitution. If the tension of the carbon dioxide in the blood is greater than the gas mixture, then the blood gives up carbon dioxide; while in the reverse case it takes up carbon dioxide from the gas mixture. The analysis of the gas mixture after passing the blood through it will also decide if the tension of the carbon dioxide in the blood is greater or less than in the gas mixture; and by a sufficiently great number of determinations, especially when THE BLOOD. 103 the amount of carbon dioxide of the gas mixture corresponds as nearly as possible in the beginning to the probable tension of this gas in the blood, we may learn the tension of the carbon dioxide in the blood. According to this method, the carbon-dioxide tension of the arterial blood is on an average 2.8$ of an atmosphere, correspond- ing to a pressure of 21 mm. mercury (STRASSBURG). In the blood from the pulmonary alveoli NUSSBAUM found a carbon-dioxide ten- sion of 3.81$ of an atmosphere, corresponding to a pressure of 28.95 mm. mercury. STRASSBUKG, who experimented on tracheotomized dogs, in which the ventilation of the lungs was less active and therefore the carbon dioxide was removed from the blood with less readiness, found in the venous blood of the heart a carbon-dioxide tension of 5.4$ of an atmosphere, corresponding to a partial pressure of 41.01 mm. mercury. Another method is the catheterization of a flap of the lungs. By the introduction of a catheter of a special construction into a branch of a bronchia the corresponding flap of the lung may be hermetically sealed, while in the other flaps of the same lung and in the other lung the ventilation remains unhindered, so that no stowing of carbon dioxide takes place in the blood. When the cutting off lasts so long that a complete equalization between the gases of the blood and the cut-off air of the lungs is assumed, a sample of this air of the lungs is removed by means of the catheter and analyzed. In the air thus obtained from the lungs NUSSBAUM and WOLFBERG found an average of 3.6$ C02. NUSSBAUM has also determined the carbon-dioxide tension in the blood of the pulmonary alveoli in a case simultaneous with the catheterization of the lungs. He found nearly identical results, namely, a carbon- dioxide tension of 3.84$ and 3.81$ of an atmosphere. While according to the just-mentioned determinations the car- bon-dioxide pressure in the venous blood amounts to about 30 mm. mercury and in the arterial to about 20 mm., BOHR has, on the con- trary, according to the above-described methods (page 101), found strikingly lower figures — 2 to 3 mm., and even lower than 1 mm. The quantity of carbon dioxide in the expired air amounts to about 2.8$ (WOLFBERG, BOHR). The air of the alveolus of the lungs is naturally richer in carbon dioxide, but the amount is not 104 PHYSIOLOGICAL CHEMISTRY. exactly known. If we start from the figures for the carbon-dioxide tension found by PFLUGER and his pupils, and when we recall, fur- ther, that NUSSBAUM found in the cut-off air of the lungs — which is more likely to be richer than poorer in carbon dioxide than the normal alveolar air of the lungs — the same carbon-dioxide tension as in the venous blood of the heart, then these observations easily agree with the view that elimination of the carbon dioxide from the blood in the lungs simply follows the laws of diffusion. According to the experiments of BOHR, in which the blood and the expired air were investigated at the same time, and in which he found the carbon-dioxide tension in the blood significantly lower than the expired air — and also lower than the alveolar air, — such an assumption as the above is impossible. BOHK also wishes to give by his experiments a proof of the view suggested long ago by LUDWIG'S school, namely, that the lungs play a specific secre- tory role in the elimination of carbon dioxide. The necessity of further investigations for the explanation of the reason for these very deviating results obtained by different investigators is ap- parent. As the carbon dioxide is always in part combined chemically, and as this part increases with the amount of alkali in the blood, it is evident that the amount of carbon dioxide and the carbon- dioxide tension must not always be parallel. That this is a fact has been shown by GAULE in experimenting on suffocated dogs. If the amount of alkali in the blood be diminished, then, naturally, the amount of carbon dioxide will be decreased. Such a behavior is found in poisoning by mineral acids. WALTER found only 2-3 vols. per cent carbon dioxide in the blood of a rabbit which had had hydrochloric acid introduced into the stomach. In the coma- tose state of diabetes melitus the alkali of the blood seems to be saturated in great part by an acid combination (ft oxy butyric acid) (STADELMANN, MINKOWSKY), and correspondingly MLFKOWSKY found in the blood in comatose diabetes only 3.3 vols. per cent carbon dioxide. In regard to the carbon-dioxide tension in the tissue, we must assume a priori that it is higher than in the blood. This is found to be true. STRASSBURG found in the urine of dogs and in the bile a carbon-dioxide tension of 9$ and 7$ of an atmosphere, respect- THE BLOOD. 105 ively. The same experimenter has, further, injected atmospheric air into a loosened intestinal knot of a living dog, and analyzed the air taken out after some time. He found a carbon-dioxide tension of 1.1% of an atmosphere. The carbon-dioxide tension in the tissue is strikingly greater than in venous blood, even if we use as basis for our calculation the figures found by PFLUGER and his pupils, as opposed to BOHR'S results of relatively high values, for the carbon- dioxide tension. It is not disputed that the carbon dioxide simply passes from the tissue to the blood according to the laws of diffusion. V. The Quantitative Constitution of the Blood. The quantitative analysis of blood cannot be of value for the blood as an entirety. We must ascertain on one side the relationship of the plasma and blood-corpuscles to each other, and on the other side the constitution of each of these two chief constituents. The diffi- culties which stand in the way of such a task, especially in regard to the living, non-coagulated blood, have not been removed. Since the constitution of the blood may differ not only in different vas- cular regions, but also in the same region under different circum- stances, which renders also number of blood analyses necessary, it can hardly appear remarkable that our knowledge of the con stitution of the blood is still relatively limited. If any substance is found in the blood which belongs exclusively to the plasma and does not occur in the blood-corpuscles, then the amount of plasma contained in the blood may be calculated if we determine the amount of this substance in 100 parts of the plasma and serum, respectively, on one side and in 100 parts of the blood on the other. If we represent the amount of this substance in the plasma by p and in the blood by 5, then the amount x of the plasma in 100 parts of blood is x — — . P Such a substance, which occurs only in the plasma, is fibrin according to HOPPE-SEYLER, sodium according to BUNGE (in cer- tain kinds of blood), and sugar according to OTTO. The experi- menters just named have tried to determine the amount of the plasma and the blood-corpuscles, respectively, in different kinds of blood, starting from the above-mentioned substances. 106 PHYSIOLOGICAL CHEMISTRY. Another method, suggested by HOPPE-SEYLER, is to determine the total amount of haemoglobin and albumin in a portion of blood, and on the other hand the amount of haemoglobin and albumin in the blood-corpuscles of an equal portion of the same blood which have been sufficiently washed with common-salt solution by centrif- ugal force. The figures obtained as a difference between these two determinations correspond to the amount of albumin which was contained in the serum of the first portion of blood. If we now determine the albumin in a special portion of serum of the same blood, then the amount of serum in the blood is easily determined. The usefulness of this method has been confirmed by BUNGE by the control experiments with the sodium determinations. If the amount of serum and blood-corpuscles in the blood is known, and we then determine the amount of the different blood-constituents in the blood-serum on one side and of the total blood on the other, the distribution of these different blood-constituents in the two chief components of the blood, plasma and blood-corpuscles may be ascertained. According to the just-mentioned procedure, the following analyses of pig's blood and ox's blood have been made by BUNGE. Analyses of human blood have been made for some time by C. SCHMIDT according to another method, which perhaps have given rather too high results for the weight of the blood- corpuscles. All figures represent parts in 1000 parts of blood. Pig's Blood. Ox's Blood. Human Blood. Man's. Woman's. Blood- cor- puscles 436.8 Serum 563.2 Blood- cor- puscles 318.7 Serum 681.3 Blood- cor- puscles 513.02 Serum 486.98 Blood- cor- puscles 396.24 Serum 603.76 Water. 276.100 160.700 151.600 5.200 3.900 2.421 517.900 45.300 38.100 2.800 4.300 0.154 2.406 0 072 191.200 127.500 123.600 2.400 1.500 0.238 0.667 622.200 59.100 49.900 3.800 5.400 0.173 2.964 0 070 349.690 163.330 j- 159. 590 3.740 1.586 0.241 439 020 47.690 43.820 4.140 0.153 1.661 272.560 123.680 120.130 3.550 1.412 0.648 551.990 51.770 46.700 5.070 0.200 1.916 Solids Haemoglobin and > Albumin j ' ' Remaining org. bodies . Inorganic bodies KQO NaoO Ca6.... MffO 0.069 '"0.657 0.903 0.021 0.006 2.034 0.106 0.005 0.031 0 007 SSoV.Y ci Y 0.521 0.224 2.532 0.181 0.898 0.695 1.722 0.071 0.362 0.643 0.144 2.202 PoOR HOPPE-SEYLER, SACHARJIX, and OTTO found 584.9-693.5 p. m. plasma and 415.1-306.5 p. m. blood-corpuscles in horse's blood. BUNGE found, on the contrary, in an analysis 468.5 p. m. serum and 531.5 p. m. blood-corpuscles — more blood-corpuscles, therefore, THE BLOOD. 107 than serum. For human blood ARRONET has found 478.8 p. m. blood -corpuscles and 521.2 p. m. serum (in defibrinated blood) as an average of nine determinations. The relative amount of blood-corpuscles and plasma therefore varies. In human blood the plasma is about 50$ of the weight of the blood; but in other cases it seems generally to be somewhat greater. In a few cases it may indeed be f of the weight of the blood. Water occurs in the greatest amount in the plasma or serum, which latter ordinarily contains at least -f$ water, while the blood- corpuscles contains only a little more than £ or about f water. Iron probably occurs only in the blood-corpuscles. Chlorine and eodium prevail generally in the plasma, potassium and phosphoric acid in the blood-corpuscles. In a few varieties of blood (pig's and horse's blood) the sodium is found exclusively in the plasma or serum, the potassium prevailing in the blood-corpuscles (BuNGE). In dog's and ox's blood the blood-corpuscles are, however, richer in sodium than in potassium (Bu^GE). In man the potassium is contained in the largest quantities in the blood-corpuscles and only in very small quantities in the plasma (C. SCHMIDT, WANACH). The alkaline earths occur chiefly in the plasma. Manganese has also been found in the blood (0.06 p. m. according to BURIN DE BUISSON"), as well as traces of lithium, copper, lead, and silver. The blood as a whole contains in ordinary cases 770-820 p. m. water, with 180-230 p. m. solids; of these 173-220 p. m. are organic and 6-10 p. m. inorganic. The organic consist, de- ducting 6-12 p. m. extractive bodies, of albumin and haemoglobin. The amount of these last-mentioned bodies in the blood is about 130-150 p. m. The amount of sugar in the blood is on an average 1-1.5 p. m. The quantity of urea, which amounts to 0.2-0.9 p. m., is greater after partaking of food than during fasting (GREHANT and QUIK- QUAUD). The quantity of uric acid may be 0.1 p. m. in bird's blood (v. SCHRODER), and the quantity of lactic acid may reach 0.71 p. m. in human venous blood (BERLINERBLAU). 108 PHYSIOLOGICAL CHEMISTRY. The Constitution of the Blood in different Vascular Regions and under different Physiological Conditions. Arterial and Venous Blood. The most striking difference be- tween these two kinds of blood is the variation in color caused by their containing different amounts of gas and different amounts of oxyhsemoglobin and haemoglobin. The arterial blood is light red ; the venous blood is dark red, dichroitic, greenish by transmitted light through thin layers. The arterial coagulates more quickly than the venous blood. The latter, on account of the transudation which takes place in the capillaries, is somewhat poorer in water but richer in blood -corpuscles and haemoglobin than the arterial blood (HEIDENHAIN, NASSE, OTTO). The quantity of sugar is somewhat greater in the arterial blood than in the venous (OTTO). Blood from the Portal Vein and the Hepatic Vein. The blood of the hepatic vein is poorer in ordinary red blood-corpuscles but richer in colorless and so-called young red blood-corpuscles. A few investi- gators have decided from this that a formation of red blood-corpus- cles takes place in the liver, while others claim that a destruction takes place. In consideration of the relationship to the simultaneous forma- tion of small quantities of bile and lymph in the unit of time of the large quantities of blood circulating throuhg the liver we can hardly expect to detect a positive difference in the constitution of the blood of the hepatic vein by chemical analysis. The statements in regard to such a difference are in fact contradictory. For example, DROSDOFF has found more haemoglobin in the hepatic than in the portal veins, while OTTO found less. In regard to the different amounts of sugar in these two kinds of blood we have also much contradiction. According to a few experimenters, as OTTO and above all others SEEGEN, the blood of the hepatic vein is richer in sugar, which corresponds with the older statements of CLAUDE BERNARD. Such a difference may, however, be caused by the operative interference in the collection of the blood from the hepatic vein (ABELES), and as a rule investigators nowadays do not seem to agree with BERNARD'S view. During the digestion of food rich in carbohydrates the blood of the portal vein may not only be richer in glucose but also contain other carbohydrates (v. MERINO. TIIE BLOOD. 109 OTTO). The amount of urea in the blood from the hepatic vein is greater than in other blood (GREHANT and QUINQUAUD). Blood of the Splenic Vein is decidedly richer in leucocytes than the blood from the splenic artery. The red blood-corpuscles of the blood from the splenic vein are smaller than the ordinary, less flattened, and show a greater resistance to water. The blood from the splenic vein is also claimed to be richer in water, fibrin, and albumin than the ordinary venous blood (BECLARD). According to v. MIDDENDORFF, it is richer in haemoglobin than arterial blood. It coagulates more slowly. The Blood from the Veins of the Glands. The blood circulates with greater rapidity through a gland during activity (secretion) than when at rest, and the outflowing venous blood has therefore during activity a lighter red color and a greater amount of oxygen. Because of the secretion the venous blood also becomes somewhat poorer in water and richer in solids. The blood from the Muscular Veins shows an opposite behavior, for during activity it is darker and more venous in its properties because of the increased absorption of oxygen by the muscles or still greater production of carbon dioxide than when at rest. Menstrual Blood has, according to an old statement, not the power of coagulating. This statement is nevertheless false, and the apparent uncoagulability depends in part on the womb and the vagina retaining the blood-clot, so that only fluid cruor is at times eliminated, and in part on a contamination with vaginal mucus which disturbs the coagulation. The Blood of the Two Sexes. Woman's blood coagulates somewhat more quickly, has a lower specific gravity, a greater amount of water, and a smaller quantity of solids than the blood of man. The amount of blood-corpuscles and haemoglobin is somewhat smaller in woman's blood. The amount of haemoglobin is, according to OTTO, 146 p. m. for man's blood and 133 p. m. for woman's. During pregnancy NASSE has observed a decrease in the specific gravity, with an increase in the amount of water towards the end of the eighth month. From then the specific gravity increases, and at delivery it is normal again. The amount of fibrin is somewhat increased (BECQUEREL and RODIER, NASSE). The number of 110 PHYSIOLOGICAL CHEMISTRY. blood-corpuscles seems to decrease. In regard to the amount of haemoglobin the statements are somewhat contradictory. The Blood at Different Periods of Life. Foetal blood is strikingly poorer in blood-corpuscles and haemoglobin than the blood of the adult. The foetal blood at the moment of birth has, according to SCHERRENZISS, a lower specific gravity, a mark- edly lower amount of haemoglobin, and a little less fibrin, but a greater amount of mineral bodies, especially proportionally more sodium (but less potassium) than the blood of adults. A few hours after birth the blood of the child has the same quantity of haemoglobin as the blood of the mother (COHNSTEIN, ZUNTZ, OTTO). The amount of haemoglobin and of blood-corpuscles then quickly increases; still they do not both increase at the same rate, as the amount of haemoglobin increases much faster. Two to three days after birth the haemoglobin reaches a maximum (20-21$), which is greater than at any other period of life. This is the cause of the great abundance of solids in the blood of new-born infants as observed by DENIS, PANUM, and other investigators. The quan- tity of haemoglobin and blood-corpuscles sinks gradually from this first maximum to a minimum of about 11$ haemoglobin, which minimum appears in human beings between the fourth and eighth year. The quantity of haemoglobin then increases again until about the twentieth year, when a second maximum of 13.7-15$ is reached. The haemoglobin remains at this point only towards the forty-fifth year, and then gradually and slowly decreases (LEICHTENSTERN, OTTO). According to older statements, the blood at old age is poorer in blood-corpuscles and albuminous bodies but richer in water and salts. The Influence of Food on the Blood. In complete starvation a decrease in the amount of solid blood constituents is found to take place (PANUM and others). The amount of haemoglobin is a little increased (SUBBOTIN, OTTO), and also the number of red blood-cor- puscles is greater (WORM MULLER, BUNTZEN), which probably depends on the fact that the blood-corpuscles are not so quickly transformed as the serum. As after-effect the inanition causes an anaemic condition (WORM MULLER, OTTO, BUNTZEN). After a rich meal the relative number of blood-corpuscles, especially after secretion of digestive juices or absorption of THE BLOOD. Ill nutritive liquids, may be increased or diminished LEICHTENSTERN). The number of colorless blood-corpuscles may be increased to such an extent, after a diet rich in albumin, that a true digestion leucocytose appears (HOFMEISTER and POHL). After a diet rich in fat the plasma becomes, even after a short time, more or less milky-white, like an emulsion. The constitution of the food acts essentially on the amount of haemoglobin in the blood. The blood of herbivora is generally poorer in haemoglobin than that from carnivora, and SUBBOTI^ has observed in dogs after a partial feeding with food rich in carbohydrates that the amount of haemoglobin sank from the physiological average of 137.5 p. m. to 103.2-93.7 p. m. According to LEICHTENSTERN, a gradual increase in the amount of haemoglobin is found to take place in the blood of human beings on enriching the food, and according to the same investigator the blood of lean persons is generally somewhat richer in haemoglobin than blood from fat ones of the same age. The addition of iron salts to the food greatly influences the number of blood-corpuscles and also the amount of haemoglobin they con- tain, so that, according to NASSE, the iron, especially in combination with fat, is active. According to the investigations of HAYEM and MALLASSEZ, the iron preparations increase the amount of haemo- globin contained in the blood in anaemia to a higher degree than the number of blood-corpuscles. The Constitution of the Blood under Physiological Conditions may be changed either by the appearance of a foreign substance or by the quantities of any one or more of the blood constituents being abnormally increased or diminished. Changes of this last kind occur frequently. An increase in the number of red corpuscles, a true " PLETHORA POLYCYTH^EMICA," takes place after transfusion of blood of the same species of animal. According to the observations of PAKUM and WORM MULLER, the blood-liquid is quickly eliminated and transformed in this case, — the water being eliminated principally by the kidneys, and the albumin burned into urea, etc., — while the blood-corpuscles are preserved longer and cause a "POLYCYTH^- MIA." A relative increase in the number of red corpuscles is found after abundant transudations from the blood, as in cholera and heart-failure, with considerable accumulation. 112 PHYSIOLOGICAL CHEMISTRY. A decrease in the number of red corpuscles occurs in anaemia from different causes. Each hemorrhage causes an acute anaemia or oligaemia. Even during the bleeding the remaining blood be- comes richer in water by diminished secretion and excretion, as also by an abundant absorption of parenchymous fluid somewhat poorer in albumin and strikingly poorer in red blood-corpuscles. The oligaemia passes soon into a hydraemia. The amount of albumin then gradually increases again; but the re-formation of the red blood-corpuscles is slower, and after the hydraemia follows also an oligocythaemia. After a little time the number of blood-corpuscles rises to normal; but the re-formation of haemoglobin does not keep pace with the re-formation of the corpuscles, and a chlorotic con- dition may appear (LAACHE, BUNTZEN, OTTO). A considerable decrease in the number of red corpuscles occurs also in chronic anaemia and chlorosis; still in such cases an essential decrease in the amount of haemoglobin occurs without an essential decrease in the number of blood-corpuscles. The decrease in the amount of haemoglobin is more characteristic of chlorosis than a decrease in the number of red corpuscles. A very considerable decrease in the number of red corpuscles (from 300,000 to 400,000 in 1 c. mm.) and diminishing in the amount of haemoglobin (from J to ^) occurs in malignant anaemia (HAYEM, LEPINE, LAACHE, and others). On the contrary, the individual red corpuscles are larger and richer in haemoglobin than they ordi- narily are, and the number stands in an inverse relationship to the amount of haemoglobin (HAYEM). The Constitution of the Red Corpuscles. Irrespective of the changes in the amount of haemoglobin, as just mentioned, the con- stitution of the blood-corpuscles may be changed in other ways. By abundant transudation, as in cholera, the blood-corpuscles may give up water, potassium, and phosphoric acid to the concentrated plasma and become correspondingly richer in organic substances (0. SCHMIDT). By a few other transudations processes, as in dys- entery and dropsy with albuminuria, a considerable amount of albumin passes from the blood, the plasma becomes richer in water, and the blood-corpuscles may take up water and so become poorer in organic substance (0. SCHMIDT). The number of colorless blood-corpuscles is found to be in- THE BLOOD. 113 creased by suppuration in puerperal fever, pyaemia, and many other diseases, but especially in leucaemia, which disease is char- acterized by a great abundance of leucocytes in the blood. The number of leucocytes is not only absolutely increased in this disease, but also in proportion to the number of red blood-cor- puscles, which is considerably diminished in leucaemia. The blood from a leucaemic patient has a lower specific gravity than the ordi- nary (1.035-1.040) and a lighter color, as if it were mixed with pus. The reaction after death is often acid, probably due to a decompo- sition of the considerably-increased lecithin. In leucaemic blood, volatile fatty acids, lactic acid, glycero-phosphoric acid, large amounts of xanthin bodies (SALOMON, KOSSEL) and the so-called CHARCOT'S crystals (see Chapter XI) have been found. The quantity of water in the blood is increased by general dropsy, with or without kidney disease, by the different forms of anaemia, by scurvy, and by febrile diseases. The amount of water is diminished by abundant transudations, by powerful laxatives, by diarrhoea, and especially by cholera. The amount of albumins in the blood may be relatively in- creased (HYPERALBUMINOSE) in cholera and after the action of laxatives. A decrease in the amount of albumin (HYPALBUMINOSE) occurs after direct loss of albumin from the blood, as in bleeding albuminuria, dysentery, abundant formation of pus, etc., etc. The amount of fibrin is increased (HYPERINOSE) in inflammatory dis- eases, pneumonia, acute muscular rheumatism, and erysipelas, in which the blood yields a " CRUSTA PHLOGISTICA " because it coagu- lates more slowly. The statements in regard to the occurrence of a hyperinose in scurvy and hydraemia seems to require further investigation. A decrease in the amount of fibrin (HYPINOSE) has been observed in malaria, pyaemia, and malignant anaemia. These statements also require further corroboration. The amount of fat in the blood (LIP^MIA) increases, irrespect- ive of the increase after a diet rich in fat, in drunkards, in corpu- lent individuals, after fracture of the bones, and also in diabetes. In the last-mentioned case the increase in fat depends, according to PAVY and HOPPE-SEYLER, upon defective digestion. An increase in the amount of fat in the blood has also been ob- served in diseases of the liver, Bright's disease, tuberculosis, mala- 114 PHYSIOLOGICAL CHEMISTRY. xia, and cholera, v. JAKSCH has observed volatile fatty acids in the blood (LIPACID^EMIA) in febrile diseases and sometimes in dia- betes. The amount of salts in the blood is increased in dropsy, dysen- tery, and in cholera immediately after the first violent attack, but diminishes later after the attack in cholera, in scurvy, and in inflammatory diseases. The decrease of alkali salts, especially com- mon salt, is only trifling, but in pneumonia the salt disappears almost entirely from the urine. A decrease in the alkalinity of the blood has been observed in many cases, as in fevers, uraemia, car- bon-monoxide poisoning, diseases of the liver, leucaemia, malignant anaemia, and diabetes. The above-mentioned (page 104) decrease in the alkalinity of the blood in diabetes mellitus is of special in- terest. The quantity of glucose is increased in diabetes (mellitaemia). HOPPE-SEYLER found in one case 9 p. m. glucose in the blood. According to CLAUDE BERNARD, when the quantity of glucose in the blood amounts to 3 p. m. it passes into the urine. The quantity of urea is augmented in fevers, also in increased exchange of albumin, and by an increased formation of urea caused thereby. A further increase in the amount of urea in the blood occurs in a retarded micturition, as in cholera as well as in cholera infantum (K. MORNER), and in affections of the kidneys and the urinary passages. After a ligature of the ureters or after extirpation of the kidneys of animals an accumulation of urea takes place in the blood. In uraemia, ammonia may occur in the blood, which origi- nates from a decomposition of the urea. Uric acid is found in- creased in the blood in gout (G-ARROD, SALOMON) ; oxalic acid was also found in the blood in the same disease by GARROD. Among the foreign bodies which are found in the blood the fol- lowing must be mentioned here: BILIARY ACIDS and BILIARY COL- ORING MATTERS (which latter may occur under physiological condi- tions in a few varieties of blood) in iceterus; LEUCIN and TYROSIN in acute atrophy of the liver; ACETON specially in fevers (v. JAKSCH). In melanaemia, especially after continuous malarial fever, black, less often light brown or yellowish, grains of pigment occur in the blood, which, according to the generally-received opinion, come into the blood from the spleen. After poisoning with potassium chlo- THE BLOOD. 115 rate, metahaemoglobin is observed in human and in canine blood (MARCHAXD and KAHN ) ; but, on the contrary, no formation of metahaemoglobin takes place in the blood of rabbits (STOKVIS and KIMMYSER). A formation of metahaemoglobin may be caused at the expense of the haemoglobin by the inhalation of amyl nitrite, as also by the action of a number of other medicinal bodies (HAYEM and others). The quantity of blood is indeed somewhat variable in different species of animals and in different conditions of the body; in gen- eral we consider the entire quantity of blood in adults as about iV~iV °^ the weight of the body, and in new-born infants about T^. Fat individuals are relatively poorer in blood than lean ones. During inanition the quantity of blood decreases less quickly than the weight of the body (PANUM), and it may therefore be also pro- portionally greater in starving individuals than in well-fed ones. By careful bleeding the quantity of blood may be considerably diminished without any dangerous symptoms. The loss of blood to J of the normal quantity has as sequence no durable sinking of the blood-pressure in the arteries; while the smaller arteries ac- commodate themselves to the small quantities of blood by contract- ing (WORM MULLER). A loss of blood to \ of the quantity reduces the blood-pressure considerably, and a loss of £ of the blood in adults is dangerous to life. The faster the bleeding the more dan- gerous it is. New-born infants are very sensitive to loss of blood, and likewise fat, old, and weak persons cannot stand much loss of blood. Women can stand loss of blood better than men. The quantity of blood may be considerably increased by the in- jection of blood from the same species of animal (PANUM, LANDOIS, WORM MULLER, PONFICK). According to WORM MULLER, the nor- mal quantity of blood may indeed be increased to 83$ without pro- ducing any abnormal conditions or continuing high blood-pressure. An increase of the quantity of blood to 150$ may be directly dan- gerous to life (WORM MULLER). If the quantity of blood of an animal is increased by transfusion with blood of the same kind of animal, an abundant formation of lymph takes place. The water in excess is eliminated by the urine; and as the albumin of the blood serum is quickly decomposed, while the red blood-corpuscles are 116 PHYSIOLOGICAL CHEMISTRY. destroyed much more slowly (TSCHIRJEW, FORSTER, PAKUM, WORM MULLER), a polycythaemia is gradually produced. If blood of another kind is transfused, then under certain con- ditions, according to the quantity of blood introduced, more or less menacing symptoms appear. These appear, for instance, when the blood-corpuscles of the receiver are dissolved easily by the serum of the introduced blood, as, for example, the blood-corpuscles of rab- bits on transfusion with a different kind of blood, or the reverse,, when the blood-corpuscles of the transfused blood are dissolved by the blood of the receiver; for instance, when the blood of a dog is transfused with rabbit's or lamb's blood, or the blood of a man with lamb's blood (LANDOIS). Before dissolving, the blood-corpuscles may unite in tough agglomerated heaps, which clog up the smaller vessels (LAKDOIS). On the other hand, the stromata of the dissolved blood-corpuscles may also give rise to an extensive intravascular coagulation causing death. The transfusion should therefore when possible be made with the blood of the same kind of animal, and for the resuscitating action of the blood it is immaterial whether or not it contains the fibrin or the mother-substance of the same. The action of trans- fused blood depends on its blood-corpuscles, and therefore defibri- nated blood acts just like non-defibrinated (PANUM, LANDOIS). The quantity of blood in the different organs depends essentially on the activity of the same. During work the exchange of mate- rial in an organ is more active than when at rest, and the increased exchange of material is combined with a more abundant flow of blood. Although the total quantity of blood in the body remains constant, the distribution of the blood in the various organs may be different at different times. As a rule, the quantity of blood in an organ can be an approximate measure of the more or less active exchange of material going on in the same, and from this point of view the distribution of the blood in the different organs and groups of organs is of interest. According to RAISTKE, to whom we are especially indebted for our knowledge of the relationship of the activity of the organs to the quantity of blood contained therein, of the total quantity of blood (in the rabbit) about £ comes to the muscles in rest, £ to the heart and the large blood-vessels, J to the liver, and \ to the other organs. CHAPTER V. CHYLE, LYMPH, TRANSUDATIONS AND EXUDATIONS. I. Chyle and Lymph. FROM the close relationship which exists between blood and lymph, and the dependence which the formation of lymph has upon the blood-circulation and the blood-pressure, it is to be expected that a close correspondence in the chemical constitution between blood- plasma and lymph should exist. The lymph is generally considered as transudated plasma. Qualitatively the lymph contains the same substances as the plasma. The essential difference is of a quantita- tive nature and consists in that the lymph is poorer in albumin. No essential chemical difference has been found between the lymph and the chyle of starving animals. After the assimilation of fatty food the chyle differs from the lymph in its wealth of minutely- divided fat-globules which give it a milky appearance; hence the old name " milk-juice." Chyle and lymph, as well as the plasma, contain serum albumin, serum globulin, fibrinogen, and fibrin ferment. The two last-men- tioned bodies occur only in very small amounts; therefore the chyle and lymph coagulate slowly (but spontaneously) and yield but little fibrin. Like other liquids poor in fibrin ferment, chyle and lymph do not at once coagulate completely, but repeated coagula- tions take place. The extractive bodies seem to be the same as in plasma. Glucose is found in about the same quantity as in the blood-serum (v. MERING), but in larger quantities than in the blood (PoiSEU- ILLE and LEFORT, GINSBERG) ; this depends on the fact that the blood-corpuscles contain no glucose. The amount of urea has been 117 118 PHYSIOLOGICAL CHEMISTRY. determined by WUETZ as 0.12-0.28 p. m. The mineral bodies appear to be the same as in plasma. As form -elements leucocytes and red blood-corpuscles are com- mon to both chyle and lymph. When it has not left the connivent valves chyle contains very few leucocytes, but in the vessels mov- ing on the peritoneal side of the intestines it is richer in leuco- cytes. The greatest quantity of leucocytes is found in the chyle between the great mesenteric gland and the cisterna chyli. The chyle is poorer in leucocytes in the thoracic duct, probably because a mixing takes place here with lymph, from other parts of the body that is poorer in form-constituents. The red blood-corpuscles occur in the chyle and lymph in very small quantities. In these liquids, which seem to be free from oxygen, the blood-corpuscles are darker-colored, and only after they have come in contact with the air do they have the light-red color of oxyhaemoglobin and give the surface of the fibrin clot a beautiful light-red appearance. It has been suggested that this red color originates from the transition forms between red and white blood-corpuscles, in which blood-coloring matters are first formed by the action of the oxygen. The chyle of starving animals has the appearance of lymph. After partaking of fat or food rich in fat it is milky, and this is partly due to the presence of large fat-globules, as in milk, or partly, and indeed chiefly, the finely-divided fat. The nature of the fats occurring in the chyle depends on the variety of fat in the food. The disproportionally greater part consists of neutral fats, and even after feeding with abundant amounts of free fatty acids MIHSTK and LEBEDEFF found in the chyle chiefly neutral fats with a small quantity of fatty acids or soaps. The gases of the chyle have not been studied, and it seems that the gases of an entirely normal human lymph have not thus far been investigated. The gases from dog-lymph contain only traces of oxygen and consist of 37.4-53.1$ C02 and 1.6$ N (AUTHOK) calcu- lated at 0° C. and 760 mm. mercury. The chief mass of the carbon dioxide of the lymph seems to be firmly chemically combined. Comparative analyses of blood and lymph have shown that the lymph contains more carbon dioxide than arterial but less than venous blood. The tension of the carbon dioxide of lymph is, CHYLE, LYMPH, TRANSUDATIONS AND EXUDATIONS. 119 according to PFLUGER and STRASSBURG, smaller than in venous but greater than in arterial blood. The quantitative constitution of the chyle must naturally be very variable. The analyses thus far made refer only to that mixture of chyle and lymph which is obtained from the thoracic duct. The specific gravity varies between 1.007 and 1.043. As example of the constitution of human chyle we will here give two analyses. The first is by OWEN- BEES, of the chyle of an executed person, and the second by HOPPE-SEYLER, of the chyle in a case of rupture of the thoracic duct. In the latter case the fibrin had previously separated. The results are in 1000 parts. No. 1. No. 2. Water 904.8 940.72 water Solids 95.1 59.28 solids Fibrin traces Albumin 70.8 36.67 albumin Fat 9.2 7.23 fat 2.35 soaps fO.83 lecithin Remaining organic bodies 10.8 ^ £jg ^hofextractives ^0.57 water extractives A A \ 6.80 soluble salts 8alts \ 0.35 insoluble salts The quantity of fat is very variable and may be considerably in- creased by partaking food rich in fats. A great many analyses of chyle from animals have been made, and they chiefly show the fact that the chyle is a liquid with a very changeable composition which stands closely related to blood- plasma, but with the chief difference that it contains more fat and less solids. The reader is referred to special works for these analyses, as, for example, to v. GORTJP-BESANEZ'S " Lehrbuch der ' physiologischen Chemie," 4th edition. The composition of the lymph is also very changeable, and its specific gravity shows about the same variation as the chyle. In the following analyses, 1 and 2, made by GUBLER and QUEVESTNE, are the results obtained from lymph from the upper part of the thigh of a woman aged 39 ; and 3, made by v. SCHERER, is an analysis of lymph from the sac-like dilated vessels of the spermatic cord. No. 120 PHYSIOLOGICAL CHEMISTRY. 4 was made by C. SCHMIDT, the data being obtained from lymph from the neck of a colt. The results are in parts per 1000. 1234 Water 939.9 934.8 957.6 955.4 Solids 60.1 65.2 42.4 44.6 Fibrin 0.5 0.6 0.4 2.2 Albumin 42.7 42.8 34.7 ) Fat, cholesterin, lecithin 3.8 9.2 L 34.9 Extractive bodies 5.7 4.4 ....) Salts 7.3 8.2 7.3 7.5 The mixture of salts found by C. SCHMIDT in the lymph of the horse has the following composition, calculated in parts per 1000 parts of the lymph : Sodium chloride 5.67 Soda 1.27 Potash '. 0.16 Sulphuric acid 0.09 Phosporic acid united with alkalies 0.02 Earthy phosphates 0.26 Under pathological conditions the lymph may be so rich in finely-divided fat that it appears like chyle. Such lymph has been investigated by HENSBN in a case of lymph fistula in a ten-year-old boy, and by LANG in a case of lymph fistula in the left upper part of the thigh of a girl of seventeen. The lymph investigated by HENSEK contained as an average of nineteen analyses 19 p. m. of fat and 0.6 p. m. of cholesterin, while that investigated by LA^G contained 24.8 p. m. of fat. The quantity of chyle and lymph must naturally change con- siderably, therefore, for this and other reasons, the calculations of the quantity in 24 hours are not to be depended upon. The food t plays a very important role in the quantity of chyle and lymph. NASSE has observed in dogs that the formation of lymph is 36$ more after feeding with meat than after feeding with potatoes, and about 54$ more than after 24 hours' deprivation of food. The amount of lymph is increased by the following influences, namely, by increasing the total quantity of blood, as by transfusion of blood (WORM MULLEK), raising the blood-pressure (LuDwiG and TOMSA), increased influx of the arterial blood (LuowiG, RAGOWICZ. GIANUZZI), and, above all, by preventing the discharge of the blood CHYLE, LYMPH, TRANSUDATIONS AND EXUDATIONS. 121 by tying the veins (BIDDER, EMMINGHAUS, WEISS). The quantity of lymph is also increased by strong active or passive muscular movements (LESSER). On poisoning with curara the secretion of lymph is increased (PuscnuTiN, LESSER), and the solids of the lymph are also increased at the same time. II. Transudations and Exudations. The serous membranes are normally kept moistened by liquids whose quantity is only sufficient in a few instances, as in the pericardial cavity and the arachnoid membrane, for a complete chemical analysis to be made of them. Under diseased conditions an abundant transudation may take place from the blood into the serous cavities, into the subcutaneous tissues, or under the epi- dermis; and in this way pathological transudations are formed. Such true transudations, which are similar to lymph, are generally poor in form-elements and leucocytes, and yield only very little or almost no fibrin, while the inflammatory transudations, the so-called exudations, are generally rich in leucocytes and yield proportionally more fibrin. As a rule, the richer a transudation is in leucocytes the closer it stands to pus, while when it has a diminished quantity of leucocytes it is more nearly like real transudations or lymph. It is ordinarily accepted that filtration is of the greatest im- portance in the formation of transudations and exudations. The facts coincide with this view, namely, that all these fluids contain the salts and extractive bodies occurring in the blood-plasma in about the same quantity as the blood-plasma, while the amount of albumin is habitually smaller. While the different fluids belong- ing to this group have about the same quantities of salts and extractive bodies, they differ from each other chiefly in containing differing quantities of albumin and form -elements, as well as varying quantities of transformation and decomposition products of these latter — changed blood-coloring matters, cholesterin, etc., etc. The largest quantity of albumin habitually occurs in in- flammatory processes with changed permeability of the walls of the vessels. The condition of the capillaries in the different vascular regions affects the amount of albumin. For example, the 122 PHYSIOLOGICAL CHEMISTRY. amount of albumin in the PERICARDIAL, PLEURAL, and PERITONEAL FLUIDS is considerably greater than in those fluids which are found in the ARACHNOID MEMBRANE, in the SUB-CUTANEOUS TISSUES, or in the AQUEOUS HUMOR. The condition of the blood also greatly affects the transudations, for in hydraemia the amount of albumin in the transudation is very small. With the increase of the age of a transudation, of a hydrocele fluid for instance, the quantity of albumin is increased, probably by resorption of water, and indeed exceptional cases may occur in which the amount of albumin, with- out any previous bleeding, is greater than in the blood-serum. It is natural to suppose that the state of the circulation and the pressure must have an influence on the quantity and composition of the transudation even though their action is little known. By increasing the vein-pressure SENATOR caused an increase in the quantity of transudation and the amount of albumin contained therein, while the amount of salts was not essentially changed. Of the variation in the amount of albumin by simple arterial hyperaemia nothing is positively known. The gases of the transudations consist of carbon dioxide besides small amounts of nitrogen and traces of oxygen. The tension of the carbon dioxide is greater in the transudations than in the blood (EwALD). On mixing with pus the amount of carbon dioxide is decreased. The extractives are, as above stated, the same as in the blood- plasma; but sometimes extractive bodies occur, such as allantoin in dropsical fluids (MOSCATELLI), which have not been detected in the blood. Urea seems to occur in very variable amounts. Glucose, or at least a substance which reduces copper oxide in alkaline liquids, occurs in most transudations. Succinic acid has been found in a few cases in hydrocele fluids, while in other cases it is entirely absent. Leucin and tyrosin have been found in transuda- tions from diseased livers and in pus-like transudations which have decomposed. Among other extractives found in transudations we must mention uric acid, allantoin, xanthin, creatin, inosit, and pyrocatechin. As above stated, irrespective of the varying number of form- elements contained in, the different transudations, the quantity of albumin is the most characteristic chemical distinction in their CHYLE, LYMPH, TRANSUDATIONS AND EXUDATIONS. 123 composition; therefore a quantitative analysis is of little impor- tance except in determining the quantity of albumin. Pericardial Fluid. The quantity of this fluid is also, under certain physiological conditions, so large that a sufficient quantity for chemical investigation was obtained from a person who had been executed. This fluid is lemon-yellow in color, somewhat sticky, and yields more fibrin than other transudations (6-8 p. m.). The amount of solids, according to the analyses performed by v. GORUP- BESANEZ, WACHSMUTH, and HOPPE-SEYLER, is 37.5-44.9 p. m.,and the amount of albumin is 22.8-24.7 p. m. In a case of chyloperi- cardium, which was probably due to the rupture of a chylus vessel or caused by a capillary exudation of chyle because of stowing, HASEBROEK found in 1000 parts of the analyzed fluid 103.61 parts Bolids, 73.79 albuminous bodies, 10.77 fat, 3.34 cholesterin, 1.77 lecithin, and 9.34 salts. The pleural fluid occurs under physiological conditions in such small quantities that a chemical analysis of the same cannot be made. Under pathological conditions this fluid may show very variable properties. In a few cases it is nearly serous, in others again sero-fibrinous, and in others similar to pus. There is a corresponding variation in the specific gravity and the properties in general. If a pus-like exudation is kept closed for a long time in the pleural cavity, a more or less complete maceration and solution of the pus-corpuscles is found to take place. The ejected yellowish- brown or greenish fluid may then be as rich in solids as the blood- serum; and an abundant flocculent precipitate of a neucleo-albumin (the pyin of early writers) may be obtained on the addition of acetic acid. This precipitate is soluble with difficulty by adding an excess of acetic acid. According to MEHU, who has investigated a great number of pleural fluids, the specific gravity is generally higher than 1.020 in acute pleurisy, the amount of solids is 6.5 p. m., and the quantity of fibrin not higher than 1.2 p. m. In chronic pleurisy with gath- ering of pus the specific gravity is higher than 1.018 and may rise to 1.024 (according to the observations of the author it may rise indeed to 1.030). The quantity of solids may in these cases be 60-70 p. m. or even more — 90-100 p. m. (AUTHOR). Fibrin is absent. In dis- turbed circulation, as in cirrhosis of the liver or in heart troubles, 124 PHYSIOLOGICAL CHEMISTRY. the specific gravity is usually lower than 1.015 and the quantity of solids averages 20-30 p. m. The quantity of peritoneal fluid is very small under physiologi- cal conditions. The investigations refer only to the fluid under diseased conditions (dropsical or ascites fluid.) The color, trans- parency, and consistency of these may vary greatly. In cachectic conditions the fluid is nearly colorless, milky-opal- escent, watery, does not coagulate spontaneously, is of a very low specific gravity, 1.005-1.015, and nearly free from form-constituents. In carcinomatous peritonitis it may have a cloudy, dirty-gray appear- ance due to its richness in form-elements of various kinds. The specific gravity is then higher, the quantity of solids greater, and it often coagulates spontaneously. In inflammatory processes it is straw- or lemon-yellow in color, somewhat cloudy or reddish, due to leucocytes and red blood-corpuscles, and from great richness in leu- cocytes it may appear more like pus. It coagulates spontaneously, may be relatively richer in solids, and may have a specific gravity of 1.030 or more. By rupture of a chylous vessel the dropsical fluid may be rich in very finely-emulsified fat (CHYLOUS ASCITES). In such cases 3.86-10.30 p. m. fat has been found in the dropsical fluid (GUINOCHET, HAY). By admixture of this fluid with the fluid from an ovarian cyst it may sometimes contain pseudomucin (see Chapter XI). We also have cases in which the ascitical fluid contains mucoids which may be precipitated by alcohol after removal of the albumins by coagulation at boiling temperature. Such substances, which yield a reducible substance on boiling with acids, have been found by the author in tuberculous peritonitis and in cirrhosis hepatis syphilitica. In order to show the amount of albumin in ascitic fluids we give below the results of RUNEBERG'S analyses. They are in parts per 1000 parts of the fluid. Max. Mia. Average. Ascitic fluid in hydraemia 4.1 0.2 2.1 " " portal obstruction 26.8 3.7 9.7 " " heart disease .......23.0 8.4 16.7 " " " carciuomatous peritonitis. 54.2 27.0 35.1 Urea has also been found in ascitical fluids, sometimes only as traces, some- times in larger quantities (4 p. m. in albuminuria), also uric acid, allantoin in cirrhosis of the liver (MOSCATELLI), xanthin, creatin, cholesterin, and glucose. CHYLE, LYMPH, TRANSUDATIONS AND EXUDATIONS. 125 Hydrocele and Spermatocele Fluids. These fluids differ from each other in various ways. The hydrocele fluids are generally colored light or darker yellow, sometimes brownish with a shade of green. They have a relatively higher specific gravity, 1.016-1.026, with a variable but generally higher amount of solids, an average of 60 p. m. They sometimes coagulate spontaneously, sometimes only after the addition of fibrin ferment or blood. They contain as form-elements chiefly leucocytes. Sometimes they contain smaller or larger amounts of cliolesterin crystals. The spermatocele fluids, on the contrary, are as a rule colorless, thin, cloudy like water mixed with milk. They sometimes have an acid reaction. They have a lower specific gravity, 1.006 to 1.010, a lower amount of solids — an average of about 13 p. m., — and do not coagulate either spontaneously or after the addition of blood. They are, as a rule, poor in albumin and contain spermatozoa, cell-detri- tus, and fat-globules as form-constituents. To show the unequal composition of these two kinds of fluids we will give the average results (calculated in parts per 1000 parts of the fluid) of 17 analy- ses of hydrocele fluids and 4 of spermatocele fluids made by the author : Hydrocele. Spermatocele. Water 938.35 986.33 Solids 61.15 13.17 Fibrin 0.59 Globulin 13.52 0.59 Serum albumin 35.94 1.82 Ether extractive bodies 4.02 ) Soluble salts 8.60V 10.76 Insoluble salts 0.66 ) In the hydrocele fluids traces of urea and a reducing substance have been found, and in a few cases also succinic acid and inosit. Cerebrospinal Fluid. This fluid, which in certain respects is more of a secretion than a transudation (C. SCHMIDT, HALLIBUR- TON), is thin, water-clear, and has a low specific gravity (1.005). It is very poor in solids, 10-15 p. m., and ordinarily contains about 10 p. m. albumin. This albumin is generally a mixture of globu- lin and albumose / occasionally some peptone occurs, and more rarely, in special cases, serum albumin appears (HALLIBURTON). An optically inactive, non-fermentable, reducible substance, seem- ingly pyrocatechin (HALLIBURTON), has been observed in this fluid. 126 PHYSIOLOGICAL CHEMISTRY. The older statement that the cerebro-spinal fluid differs from the transudations in a greater wealth of potassium salts has not been confirmed by recent investigations. Aqueous Humor. This fluid is clear, alkaline, and has a specific gravity of 1.003-1.009. The amount of solids is on an average 13 p. m. and the amount of proteids only 0.8-1.2 p. m. The proteids consists of about equal parts serum globulin and globulin (KAHN). According to GRUENHAGEN, it contains paralactic acid, another dextro-gyrate substance, and a reducible body which is not similar to glucose or dextrine. Blister-fluid. The content of blisters caused -by burns, and of yesicator blisters and the blisters of the pemphigus chronicus, is generally a fluid rich in solids and albumin (40-65 p. m.). This is especially true of the contents of vesicatory blisters, which also contain a substance that reduces copper oxide. The fluid of the pemphigus is slimy and alkaline in reaction. The fluid of subcutaneous 03dema. This is, as a rule, very poor in solids, purely serous, does not contain fibrinogen, and has a spe- cific gravity of 1.005 to 1.010. The quantity of proteids is in most cases lower than 10 p.m., — according to HOFFMANN 1-8 p.m., — and in serious affections of the kidneys, generally with amyloid degen- eration, less than 1 p. m. has been shown (HOFFMANN). The oadema fluid also habitually contains urea, 1-2 p. m., and also a reducible substance. The FLUID OP THE TAPEWORM cyst is related to the transudations. It is poor in proteids, thin and colorless, and has a specific gravity of 1.005- 1.015. The quantity of solids is 14-20 p. m. The chemical constituents are glucose (2.5 p. m.), inosit, traces of urea, creatin, succinic acid, and sails (8.3- 9.7 p. m.). Albumin is only found in traces, and then only after an inflam- matory irritation. Jn the last-mentioned case 7 p. m. albumin has been found in the fluid. The Synovial Fluid and Fluid in Synovial Cavities around Joints, etc. The synovia is hardly a transudation, but it is often treated as an appendix to the transudations. The synovia is an alkaline, sticky, fibrous, yellowish fluid which is cloudy, from the presence of cell-nuclei and remains of destroyed cells, but is sometimes clear. It contains also, besides albumin and salts, a mucin-like nucleoalbumin. The presence of pure mucin has not been shown. The composition of synovia is CHYLE, LYMPH, TRANS UDA TION8 AND EXUDATIONS. 127 not constant, but varies in rest and in motion. In the last- mentioned case the quantity of fluid is less, but the amount of the mucin-like body, albumin, and of the extractive bodies is greater, while the quantity of salts is diminished. This may be seen from the following analyses by FRERICHS. The figures represent parts V per 1000. I. Synovia from II. Synovia from a btall-fed Ox. a Field-fed Ox. Water 969.9 948.5 Solids 80. 1 51.5 Mucin-like body 2.4 5.6 Albumin and extractives 15.7 35.1 Fat 0.6 0.7 Salts 11.3 9.9 The synovia of new-born babes corresponds to that of resting animals. The fluid of the brusae mucosae, as also the fluid in the synovial cavities around joints, etc., is similar to synovia from a qualitative standpoint. III. The Pus. Pus is a yellowish-gray or yellowish-green, creamy mass of a faint odor and an unsavory, sweetish taste. It consists of a fluid, the pus-serum, in which solid particles, the pus-cells, swim. The number of these cells varies so considerably that the pus may at one time be thin and at another time so thick that it scarcely con- tains a drop of serum. The specific gravity, therefore, may also greatly vary, namely, between 1.020 and 1.040, but ordinarily it is 1.031-1.033. The reaction of fresh pus is generally alkaline, but it may become neutral or acid from a decomposition in which fatty acids, glycero-phosphoric acid, and also lactic acid are formed. It may become strongly alkaline when putrefaction occurs with the formation of ammonia. In the chemical investigation of pus the pus-serum and the pus-corpuscles must be studied separately.. Pus-serum. Pus does not coagulate spontaneously nor after the addition of defibrinated blood. The fluid in which the pus-corpuscles are suspended is not to be compared with the plasma, but rather with the serum. The pus-serum is pale yellow, yellowish green, or brownish yellow, and has an alkaline reaction. It contains, for the most part, the same constituents as the blood- 128 PHYSIOLOGICAL CHEMISTRY. serum; but sometimes besides these — when, for instance, the pus has remained in the body for a long time, — it contains a nucleo- albumin which is precipitated by acetic acid and soluble with great difficulty in an excess of the acid (pyin of the older authors). This nucleoalbumin seems to be formed from the hyaline substance of the pus-cells by maceration. The pus-serum contains, moreover, at least in many cases, no fibrin ferment. According to the analy- ses of HOPPE-SEYLEB, the pus-serum contains in 1000 parts: I. II. Water 913.7 905.65 Solids , 86.3 94.35 Albuminous bodies 63 23 77.21 Lecithin 1.50 056 Fat 0.26 0.29 Cholesterin 0.53 0.87 Alcohol extractives 1.52 0.73 Water extractives 11.53 6.92 Inorganic salts 7.73 7.77 The ash of pus-serum has the following composition, calculated to 1000 parts of the serum : I. II. NaCl 3.22 5.39 Na2S04 0.40 031 Na2HP04 0.98 0.46 Na2CO3 0.49 1.13 Ca3(PO4)2 . . . . 0.49 0.31 Mg3(PO4)2 0.19 0.12 PO4 (in excess) .05 The pus-corpuscles are generally thought to consist in great part of emigrated colorless blood-corpuscles (emigration hypothesis), and their chemical properties have therefore been given above. We consider the molecular grains, fat-globules, and red blood-corpuscles rather as casual form-elements. The pus-cells may be separated from the serum by centrifugal force or by decantation directly or after dilution with a solution of sodium sulphate in water (1 vol. saturated sodium-sulphate solution and 9 vols. water), and then washed by this same solution in the same manner as the blood-corpuscles. The chief constituents of the pus-corpuscles are albuminous bodies of which the largest proportion seems to be a nucleoalbumi- ous substance which is insoluble in water and which expands into a tough, slimy mass when treated with a 10# common-salt solution. This protein substance, which is soluble in alkali but quickly CHYLE, LYMPH, TRAN8UDATION8 AND EXUDATIONS. 129 changed thereby, is called ROVIDAS'S hyaline substance, and the property of the pus of being converted into a slime-like mass by a solution of common salt depends on this substance. Besides this substance we find in the pus-cells also an albuminous body which coagulates at 48-49° C., as well as serum-globulin (?), serum- albumin, a substance similar to coagulated albumin (MiESCHER), and lastly peptone (HOFMEISTER). We also find in the protoplasm of the pus-cells, besides the proteids, lecithin, cholesterin, xanthin bodies, fat, soaps, and cerebrin (see Chapter X). HOPPE-SEYLER claims that glycogen appears only in the living, contractile white blood-cells and not in the dead pus-corpuscles. SALOMON has nevertheless found glycogeu in pus. The cell-nucleus contains nuclein and some lecithin. The mineral constituents of the pus-corpuscles are potassium, sodium, calcium, magnesium, and iron. A part of the alkalies is found as chlorides, and the remainder, as well as the other bases, ex- ists as phosphates. The quantitative composition of the pus-cells from the analyses of HOPPE-SEYLER is as follows, in parts per 1000 of the dried substance : I. II. Albuminous bodies ................... 137.62 ) Nuclein ........................... 342.57 V 685.85 673.69 Insoluble bodies ...................... 205.66 ) Cholesterin ........................... 74.00 72.83 Cerebri ............................ 51.99) 1Q1 84 Extractive bodies ..................... 44.33 \ MINERAL SUBSTANCES. NaCI .............. .................................... 4.35 205 traces (?) MIESCHER has obtained other results for the alkali combinations, name- ly : potassium phosphate 12, sodium phosphate 6.1. earthy phosphate and iron phosphate 4.2, sodium chloride 1.4, and phosphonc acid combined organic substances 3.14-2.03 p. m. In pus from congested abscesses which have stagnated for some time we find peptone, leucin and tyrosin, free fatty acids and 130 PHYSIOLOGICAL CHEMISTRY. volatile fatty acids, such as formic acid, butyric acid, valerianic acid. We also sometimes find chondrin (?) and glutin (?), urea, glucose (in diabetes), biliary coloring matters, and bile acids (in catarrhal icterus). As more specific but not constant constituents of the pus we must mention the following : pyin, which seems to be a nucleo- albumin precipitable by acetic acid, and also pyinic acid and chlor- rhodinic acid, which have been so little studied that they cannot be more fully treated here. In many cases a blue, more rarely a green color, has been observed in the pus. This depends on the presence of a variety of vibrios (LUCKE) from which FORDOS and LUCKE have isolated a crystalliz- able coloring matter partly blue and partly yellow, pyocyanin and pyoxanthose. Appendix. Lymphatic Glands, Spleen, etc. The Lymphatic Glands. According to FOSTER and LANKESTER and HALLIBURTON, we find in the cells of the lymphatic glands the four albuminous bodies previously mentioned (Chapter III, page 42). Albumoses and peptones may also occur as products of a post-mortem decomposition. Besides the other ordinary tissue-constituents, such as collagen, elastin, and nuclein, we find in the lymphatic glands also cholesterin, fat, glycogen, xanthin bodies and adenin (KRONECKER), and leucin. In the inguinal glands of an old woman OIDTMAKK found 713.84 p. m. water, 285 p. m. organic and 1.16 p. m. inorganic substances. The Spleen. The pulp of the spleen cannot be freed from blood. The mass which is separated from the spleen capsule and the structural tissue by pressure and which ordinarily serves as material for chemical investigations is therefore a mixture of blood and spleen constituents. For this reason the albuminous bodies of the spleen are little known. As characteristic constituents we have albuminates containing iron and especially a protein substance which does not coagulate on boiling and which is precipitated by acetic acid and yields an ash containing much phosphoric acid and iron oxide (SOBERER). CHYLE, LYMPH, TRANSUDATIONS AND EXUDATIONS. 131 The pulp of the spleen, when fresh, has an alkaline reaction but quickly turns acid, due partly to the formation of free paralac- tic'acid and partly perhaps to glycero-pliosphoric acid. Besides these two acids there have been found in the spleen also volatile fatty acids, as formic, acetic, and butyric acids, as well as succinic acid, neutral fats, cholesterin, traces of leucin, inosit (in ox-spleen), scyllit, a body related to inosit (in the spleen of plagiostoma), glycogen (in dog-spleen), uric acid, yuanin, hypoxanthin, xanthin, adenin (KRONECKER), smdjecorin (BALDI). Among the constituents of the spleen the deposit rich in iron, which consists of ferruginous granules or conglomerate masses of them, and closely studied by NASSE, is of special interest. These iron grains developed by the changing of the red corpuscles, and which also occur in old thrombi, are chiefly produced when stag- nant blood-corpuscles are not dissolved, and they may be formed either extra-cellular or intracellular when the blood-corpuscles of the colorless cells are taken up. This deposit does not occur to the same extent in the spleen of all animals. It is found especially abundant in the spleen of the horse. NASSE on analyzing the grains (from the spleen of a horse) obtained 839.2 p. m. organic and 160.8 p. m. inorganic substances. These last consisted of 566-726 p. m. Fe203, 205-388 p. m. P206 , and 57 p. m. earths. The organic sub- stances consisted chiefly of proteids (660-800 p. m.), nuclein, 52 p. m. (maximum), a yellow coloring matter, extractive bodies, fat, cholesterin, and lecithin. In regard to the mineral constituents it is to be observed that the amount of iron is strikingly large, and further that the amount of sodium and phosphoric acid is smaller than that of potassium and chlorine. The amount of iron in new-born and young ani- mals is small (LAPICQUE), in adults more appreciable, and in old animals sometimes considerable. NASSE found nearly 50 p. m. in the dried pulp of the spleen of an old horse. The quantitative analyses of the human spleen by OIDTMANN give the following results : In men he found 750-694 p. m. water and 250-306 p. m. solids. In that of a woman he found 774.8 p. m. water and 225.2 p. m. solids. The quantity of inorganic bodies was in men 4.9-7.4 p. m., and in women 9.5 p. m. In regard to the pathological processes going on in the spleen 132 PHYSIOLOGICAL CHEMISTRY. we must specially recall the abundant re-formation of leucocytes in leucaemia and the appearance of amyloid substance (see page 39). The physiological functions of the spleen are little known. Some consider the spleen as a melting organ o£ the red blood-cor- puscles (KOLLIKER, ECKER), and the occurrence of the above-men- tioned iron deposit seems to confirm this view. Some (GERLACH, FUNKE, and others) regard the spleen as a blood-forming organ. Other investigators consider that steps in the modelling of the red blood-corpuscles occur in the spleen or that young red blood-cor- puscles occur in the blood of the splenic vein. The spleen has also been claimed to play an important part in digestion. The organ is known to enlarge after a meal, and this enlargement is thought by SCHIFF and HERZEK to be dependent upon the filling of the pancreas with enzymes. According to the above-mentioned investigators, after the extirpation of the spleen the pancreas does not produce any enzyme, which digests albumin, but HEIDENHEIM and EWALD have not been able to confirm this fact. According to later investigations of HERZEN, an enzyme which digests albumin is produced in the spleen during its enlarge- ment. An increase in the quantity of uric acid eliminated occurs in leu- caemia (RANKE, SALKOWSKI, FLEISCHER and PENZOLDT, and STADT- HAGEN)", while the reverse ol this takes place under the influence of quinine, which produces an enlargement of the spleen. We have here a rather positive proof that there is a close relationship between the spleen and the formation, of uric acid. If we assume that the xanthin bodies are steps to the formation of uric acid, then the in - crease in the uric acid in leucaemia may perhaps depend on the in- creased amount of xanthin bodies (hypoxanthin) in the spleen in this disease.* The spleen has the same property as the liver of retaining foreign bodies, metals and metalloids. The Thymus has been little studied. Besides proteids and sub- stances belonging to the connective group, we find small quantities * HOKBACZEWSKI has lately found in the spleen the first steps in the for- mation of uric acid, and he has also shown that when the spleen and blood of a calf are allowed to act on each other at the temperature of the blood and in the presence of air, large quantities of uric acid are formed. CHYLE, LYMPH, TRAN8UDATION8 AND EXUDATIONS. 133 of fat, leucin, succinic acid, lactic acid, and glucose. The large quantity of xanthin bodies, chiefly adenin, is remarkable — 179 p. m. in the fresh gland, or 19.19 p. m. in the dried substance (KossEL and SCHINDLER). Potassium and phosphoric acid are the promi- nent mineral constituents. OIDTMANN found 807.06 p. m. water, 192.74 p. m. organic and 0.2 p. m. inorganic substances in the gland of a 14-days-old child. The Thyroid Gland. The chemical constituents of this gland are little known. BUB^OW has obtained a protein substance called by him " thyreoproteine," by extracting the gland with common-salt solution or by very dilute caustic potash. This body has about the same amount of nitrogen but smaller amounts of carbon and hydrogen than the proteids in general. The fluid found in the vesicle sometimes contains a mucin-like substance which is pre- cipitated by an excess of acetic acid. Besides these, other sub- stances have been found in the extract of the glands, such as leucin, xanthin, hyper xanthin, lactic and succinic acids. OIDTMANN found in the thyroid gland of an old woman 822.4 p. m. water, 176.7 p. m. organic and 0.9. p. m. inorganic substances. He found 772.1 p. m. water, 223.4 p. m. organic and 4.5 p. m. inorganic substances in an infant 14 days old. In " STRUMA CYSTICA " HoFPE-SEYiER f ound hardly any albu- min in the smaller glandular vessels, but an excess of mucin, while in the larger he found a great deal of albumin, 70-80 p. m. Cholesterin is regularly found in such cysts, sometimes in such large quantities that the entire contents form a thick mass of cholesterin plates. Crystals of calcium oxalate also occur frequently. The contents of the struma cysts are sometimes of a brown color due to decomposed coloring matter, methaemoglobin (and haematin ?). Bile-coloring matters have also been found in such cysts. (In regard to the paralbumins and colloids which have been found in struma cysts and colloid degeneration, see Chapter XL) Little is known in regard to the functions of the thyroid gland. From a chemical standpoint the view is worth suggesting that the so-called myxoedema, which is a slimy infiltration of the subcuta- neous cell-tissue of the head and throat (besides other disturbances) stands in connection with the failing of the activity of the thyroid gland. HORSLEY and HALLIBURTON found in monkeys, but not in 134 PHYSIOLOGICAL CHEMISTRY. pigs, that the amount of mucin in the tissue was increased after extirpating the thyroid gland. The Suprarenal Body. Besides proteids, substances of the connective tissue and salts, we have found in the suprarenal body palmitin, lecithin, neurin, and glycero-phosphoric acid, which last gives the poisonous properties of the watery extract of the gland (MABiNO-Zuco and GUARKIERI), and some leucin, which is probably a decomposition product. The statement that benzoic acid, hippuric acid, biliary acids, and taurin occur in this gland requires further confirmation. In the medulla there have been found one or more chromogens which are converted into a red coloring mat- ter by the action of the air, light, warmth, haloid or metallic salts (VULPIAN, KRUKENBEKG). Pyrocatechin also probably occurs therein. Because of the amount of chromogen contained in the suprarenal body, a connection is claimed between the abnormal deposition of pigment in the skin, which is characteristic of ADDI- so^'s disease, and the diseased changes which often occur in the suprarenal body. CHAPTER VI. THE LIVER. THE liver, which is the largest organ of the body, stands in close relationship to the blood-forming organs. The importance of this organ in the physiological composition of the blood is evi- dent from the fact that the blood coming from the digestive tract, laden with absorbed bodies, must circulate through the liver before it is driven by the heart through the different organs and tissues. It has been proved, at least for the carbohydrates, that an assimila- tion of the absorbed nutritive bodies which are brought to the liver by the blood of the portal vein takes place in this organ. The occurrence of synthetical processes in the liver has been posi- tively proved by special observations. It is possible that in the liver certain ammonia combinations are converted into urea or uric acid (in birds), while certain products of putrefaction in the intestines, such as phenol, may be converted by synthesis into ethereal sul- phuric acids by the liver (PFLUGER and KOCHS). The liver has also the property of removing and retaining heterogeneous bodies from the blood, and this is not only true of metallic salts, which are often retained by this organ, but also, as SCHIFF, LAUTENBERGER, JACQUES, HEGER, and ROGER have shown, the alkaloids are re- tained and are probably partially decomposed in the liver. Even though the liver is of assimilatory importance and purifies the blood coming from the digestive tract, it is at the same time a secretory organ which eliminates a specific secretion, the bile, in the production of which the red blood-corpuscles are destroyed, or at least one of their constituents, the haemoglobin, is transformed. It is generally admitted that the liver acts contrariwise during foatal life, at that time forming the red blood-corpuscles. 185 136 PHYSIOLOGICAL CHEMISTRY. There is no doubt that the chemical operations going on in this organ are manifold and must be of the greatest importance for the organism; but unfortunately we know very little about the kind and extent of these processes. Among them are two principal ones which will be fully treated in this chapter, after we have first described the constituents and the chemical composition of the liver. One of these processes seems to be of an assimilatory nature and refers to the formation of glycogen, while the other refers to the production and secretion of the bile. The reaction of the liver-cell is alkaline during life, but becomes acid after death. This change is probably due to the formation of lactic acid, causing a coagulation of the albumins of the protoplasm of the cell. A positive difference between the albuminous bodies of the dead and the living, non-coagulated protoplasm has not been observed. The albuminous bodies of the liver were first carefully investi- gated by PLOS'Z. He found in the watery extract of the liver an albuminous substance which coagulates at -f- 45° C., also a globulin which coagulates at + 75° C., a nucleo-albumin (?) which coagu- lates at + 70° C., and lastly an albuminous body which is nearly related to coagulated albumin and which is insoluble in dilute acids at the ordinary temperature, but dissolves on the application of heat, being converted into an albuminate.1 ST. ZALESKI found in the liver an albuminous body containing iron, in which the iron is more or less strongly combined, but it is unknown what relation this bears to the albuminous bodies isolated by PLOS'Z. The fat of the liver occurs partly as very small globules and partly, especially in nursing children and sucking animals, as also after food rich in fat, as rather large fat-drops. This infiltration of fat, which may be made so abundant by proper food that it ap- pears similar in the highest degree to a pathological fatty liver, begins in the periphery of the acini and extends towards the cen- tre. If the amount of fat in the liver is increased by an infiltra- tion, the water decreases correspondingly, while the quantity of the other solids remains little changed. In fatty degeneration this is different. In this process the fat is formed from the protoplasm of the cell, and the quantity of the other solids is therefore dimin- ished while the amount of water is only slightly changed. To THE LIVER. 137 illustrate this, we give below the results from a normal liver, and also the results obtained by PERLS in fatty degeneration and fatty infiltration. The results are in 1000 parts. Normalliver Fatty degeneration 810 87 97 Fatty infiltration 620 190-240 184-145 Among the extractive substances besides glycogen, which will be treated of later, we find rather large quantities of xanthin bodies. KOSSEL found in 1000 parts of the dried substance 1.97 p. m. guanin, 1.34 p. m. hypoxanthin, and 1.21 p. m. xanthin. Adenin is also contained in the liver. In addition there have been found urea and uric acid (especially in birds), and indeed in larger quantities than in the blood, paralactic acid, leucin, jecorin, and, in pathological cases, inosit, tyrosin, and cystin. The occurrence of bile-coloring matters in the liver-cell under normal conditions is doubtful ; but in retention of the bile the cells may absorb the coloring matter and become colored thereby. Jecorin was first found by DRECHSEL in the liver of a horse, and later by BALDI in the liver and spleen of other animals, in the muscles and blood of the horse, and in the human brain. It contains sulphur and phosphorus, but its constitution is not positively known. Jecorin dissolves in ether, but is precipitated from this solution by alcohol. It reduces copper oxide, and it solidities after boiling with alkalies to a gelatinous mass. It may lead to errors in the investigations of organs or tissues, for it can easily be mistaken for lecithin on account of its solubilities and because it contains phosphorus. The mineral bodies of the liver consist of phosphoric acid, potassium, sodium, alkaline earths, and chlorine. The potassium is in excess of the sodium. Iron is a regular constituent of the liver, but in very variable amounts, 0.3-11.8 p. m. calculated for the dried substance of the liver (ST. ZALESKI). A part of the iron exists as phosphate, and the greater part in combination with the protein bodies (ST. ZALESKI). Copper seems to be a physio- logical constituent. Foreign metals, such as lead, zinc, and others, are easily taken up and retained for a long time by the liver. v. BIBEA found in 1000 parts of the liver of a young man who had suddenly died 762 p. m. water and 238 p. m. solids, consist- ing of 25 p. m. fat, 152 p. m. albumin and gelatine-forming sub- stance, and 61 p. m. extractive substances. 138 PHYSIOLOGICAL CHEMISTRY. Glycogen and the Glycogen Formation. Glycogen was discovered in 1857 by BERNARD and HENSEN in- dependently of each other. It is a carbohydrate closely related to the starches or dextrins with the general formula C6H1005, perhaps 6(C6H1006) + H20 (KuLZ and BOENTEAGEE). The largest quanti- ties are found in the liver of full-grown animals (BERNARD, HEN- SEN, and others), and smaller quantities in the muscles (NASSE, BRUCKE, and others). It is found in very small quantities in many organs, such as the lungs, skin, the sheath of the roots of the hair (WIERSMA, BARFUTH), the middle coat of the arteries, and also in certain epithelial cells (SCHIELE, WIERSMA). Its occurrence in lymphoid cells and in pus has been mentioned in the previous chapter. Glycogen has been shown by BERNARD and KUHNE to be very widely diffused in the embryonic tissue, and it seems habitually to be a constituent of tissues in which a rapid cell-formation and cell-development is taking place (HOPPE-SEYLER). It is also present in rapidly-formed pathological swellings (HOPPE-SEYLER). It has been found in several organs in diabetes mellitus. Glycogen is also found in the plant kingdom in the myxomycetae. The quantity of glycogen in the liver, as also in the muscles, de- pends essentially upon the food. In starvation it disappears after a short time, but more rapidly in small than in large animals. Ac- cording to the old views (LUCHSINGER), it disappears earlier from the muscles than from the liver; but according to more modern views (ALDEHOFF), the reverse of this takes place. After partaking of food especially rich in carbohydrates, the liver becomes rich again in glycogen, the greatest increment occurring 14 to 16 hours after eating (KuLz). The quantity of glycogen in the liver may be 100-120 p. in. or indeed even more after the consumption of food rich in carbohydrates. Ordinarily it is considerably less or 12-30 to 40 p. m. Glycogen forms an amorphous, white, tasteless, and inodorous powder. It gives an opalescent solution with water which, when al- lowed to evaporate in the water-bath, forms a pellicle over the surface that disappears again on cooling. The solution is dextro-gyrate, (a] D = -{- 211° (KuLz). The specific rotary power is given some- what differently by various investigators. A solution of glycogen THE LIVER. 139 is colored wine-red by iodine. It may hold copper oxyhydrate in solution in alkaline liquids, but does not reduce it. A solution of glycogen in water is not precipitated by potassium-mercuric iodide and hydrochloric acid, but is precipitated by alcohol or ammoniacal lead acetate. Glycogen seems to be changed somewhat by prolonged boiling with dilute caustic potash (VINTSCHGAU and DIETL). It is converted into glucose by diastatic enzymes and also by being boiled with dilute mineral acids. The preparation of pure glycogen (simplest from the liver) is generally performed by the method suggested by BRUCKE, of which the main points are the following: Immediately after the death of the animal the liver is thrown into boiling water, then finely divided and boiled several times with fresh water. The filtered extract is now sufficiently concentrated, allowed to cool, and the albumin removed by alternately adding potassium-mercuric iodide and hydrochloric acid. The glycogen is precipitated from the filtered liquid by the addition of alcohol until the liquid contains 60 vols. per cent. The glycogen is first washed on the filter with 60$ and then with 95$ alcohol, then treated with ether and dried over sulphuric acid. It is always contaminated with mineral substances. To be able to extract the glycogen from the liver or especially from muscles and other tissues completely, which is essential in a quanti- tative estimation, these parts must first be boiled for a few hours with a dilute solution of caustic potash, say 4 gms. KOH to 100 gms. liver (KtJLz). The quantitative determination is ordinarily performed accord- ing to the above method of BRUCKE, fyit care must be taken that the precipitate obtained by the potassium-mercuric iodide and hydro- chloric acid is removed at least four times from the filter and stirred with water to which has been added a few drops of hydrochloric acid and potassium-mercuric chloride, and refiltered so as to be cer- tain that all the glycogen is obtained in the filtrate (KiJLz). The quantity of glycogen may also be determined by the polariscope or by titration after first converting the glycogen into glucose by boil- ing with an acid. Numerous investigators have endeavored to determine the origin of glycogen in the body. The quantity of glycogen in the liver is increased after partaking of many substances, in the first place by the varieties of sugar and several other carbohydrates (PAVY and others), also by glycerin (VAN BEEN, WEISS, LUCHSINGER), gelatin (WOROSCHILOFF), and the glucoside arbutin. Inosit (KuLz) and mannit (LUCHSINGER) have, on the contrary, no action. Fat is 140 PHYSIOLOGICAL CHEMISTRY. claimed by most investigators to have no influence. The views in regard to the importance of the proteids in the glycogen formation are somewhat divided. From many observations, particularly certain nutrition experiments with boiled meat (NAUNYN) or blood-fibrin (v. MERIKGS), there is no doubt that the proteids are concerned in the formation of glycogen. WOLFFBERG has also found that he obtained a larger glycogen production from a diet of proteids and carbohydrates in proper proportions than from a diet consisting only of carbohydrates with very little proteids. It has been shown by many observers, and lately also by MOSZEIK, that a diet of proteids with carbohydrates caused a greater increase in the glycogen than a diet of carbohydrates alone. The great importance of the carbohydrates in the formation of glycogen has given rise to the opinion that the glycogen in the liver is produced from other carbohydrates (glucose) by a synthesis with the separation of water with a formation of anhydride (LucHSLNT- GER and others). This theory (anhydride theory) has found oppo- nents because it neither explains the formation of glycogen from such different bodies as albumin, carbohydrates, glycerine, and others, nor the circumstance that the glycogen is always the same, independent of the properties of the carbohydrate introduced, whether it is dex- tro- or laevo-gyrate. It is therefore the opinion of many investi- gators that all glycogen is formed from proteid, and that this splits into two parts, one containing nitrogen and the other free from nitrogen: the latter is the glycogen. According to these views, the carbohydrates act only in that they spare the proteid and the gly- cogen produced therefrom (WEISS, WOLFFBERG, and others). In many animal tissues we have proteids from which carbohy- drates or closely-related bodies may be split off. The occurrence in the liver of such proteids, from which carbohydrates can be split off, is from certain observations not at all improbable, but still it has not been fully proved. Nor has the view of certain investigators, that in the ordinary sense we have a carbohydrate group preformed in the albuminous body, been conclusively established. Under such circumstances it is not easy to explain the formation of glycogen in the animal body from proteids. But as it seems to be certain that glycogen can be produced either from proteids or from carbo- hydrates, which has lately been further demonstrated by E. VOIT, UITITBRSITT the opinion expressed by PFLUGER has unqueSniEftjQnSeen mis- leading. As the fat may be formed partly from proteids and partly from carbohydrates by a synthesis after previous splitting, PFLUGEB claims that the glycogen in the liver may also be produced from dif- ferent substances by a complex splitting and synthesis. There is no doubt that the glycogen of the liver, which surrounds the nu- cleus of the liver-cells as amorphous masses, is formed in these cells. Where does the glycogen occurring in the other organs, such as the muscles, originate ? Is the glycogen of the muscles formed on the spot or is it transmitted from the liver to the muscles by means of the blood? These questions cannot yet be answered with positive- ness, and the investigations on this subject by different experimen- ters (on frogs by KULZ and on birds by LAVES and MINKOWSKY) have given contradictory results. Glycogen is considered as a reserve nutritive substance deposited in the liver, and, according to the ordinary view, it is transported by the blood from the liver to the other organs, especially to the muscles, where it serves as a source of material for work. The im- portance of glycogen in the formation of heat follows from the fact that on cooling the animal body the glycogen is quickly exhausted. The possibility that fats may be formed from glycogen, as well as from other carbohydrates, cannot be denied. The relationship of glycogen to the formation of sugar is of spe- cial interest. In a dead liver the glycogen is rapidly converted into sugar, and this fact naturally leads to the supposition that we have a sugar formation from glycogen in the liver during life under nor- mal conditions, a vitale Glykogenie (AUTHOR). As proof of this, CL. BERNARD has found that the liver, under physiological conditions, always contains some sugar, and also that the blood from the hepatic vein is always somewhat richer in sugar than the blood from the portal vein. The correctness of either or both of these statements has been disputed by many investigators, such as PAVY, HITTER, SCHIFF, EULESTBERG, LussANA, ABELES and others. It is not denied that the blood from the hepatic vein may not contain some- what more sugar under certain circumstances, but it is probably due to the result of the experiment. It is impossible to discuss more completely the numerous works which treat of this question, and it is perhaps sufficient to say here 142 PHYSIOLOGICAL CHEMISTRY. that the two above-mentioned opinions stand to-day in opposition to each other. The existence of a vital formation of sugar from the glycogen of the liver is denied by certain investigators, but ad- mitted by others. Those who admit this formation claim that it is produced by the action of an enzyme which is formed in the blood, especially on the destruction of the red blood-corpuscles (TIEGEL). Other investigators, such as FOESTEE, EVES, DASTEE and others, deny the action of an enzyme, and are of the opinion that the for- mation of sugar is produced by a vital action of the protoplasm of the living cell. SEEGEIT claims that the sugar formation in the liver occurs on a very large scale under physiological conditions, and that the blood of the hepatic vein is considerably richer in sugar than the blood of the portal vein. He also claims that the sugar is not formed from the glycogen, but from the peptones and fat. The observa- tions which form the basis of this theory have not been confirmed by other investigators (CHITTENDEJT and LAMBEET). The question as to a physiological formation of sugar in the liver is disputable and not settled. There is no doubt that in cer- tain lesions of the nervous system, by poisoning, etc., an abundant formation of sugar may appear, which, at least in certain cases, is derived from the glycogen of the liver, and several investigators consider with CL. BEE^AED this sugar formation as well as the elimination sugar in diabetes mellitus, as an increase in the normal formation of sugar from the glycogen. A discussion of the different views in regard to glycosuria and diabetes mellitus is beyond the plan and scope of this book. The appearance of glucose in the urine is a symptom which under different conditions may have essentially different causes. Under all circumstances it is necessary to carefully differ- entiate between those diseased conditions on the one side which are grouped under the name diabetes mellitus and the experi- mental production of glycosuria on the other side. In diabetes, at least in most cases, we are more probably dealing with a decreased burning-up of sugar in the organism than an increased production of sugar from the glycogen of the liver, or a disturbed storing-up of glycogen in this organ. On the contrary, in experimental gly- cosuria in certain cases we have undoubtedly a formation of sugar THE LIVER. 143 from the glycogen of the liver. As proof thereof, in the so-called " Zuckerstich " (lesion of a certain part of the fourth ventricle of the brain) no glycosuria is produced in animals with glycogen-free livers (fasting animals), while in livers containing glycogen it is found rapidly disappearing with the formation of sugar after this operation (HERMANN and DOCK, LUCHSINGER). VON MERINGS has shown by experiments that we may have a glycosuria formed independently of the glycogen of the liver. This investigator has shown by experiments on dogs that in animals which have been starving for some time, so that the liver, as well as the muscles, is free from glycogen, a very considerable glycosuria is produced on administering the glucoside phloridzin. The elimination of sugar is considerably greater by this means than that produced by the de- composition of the glucoside itself. VON MERINGS has been able to produce diabetes by this glucoside in geese which have had their livers removed ; and LANGENDORFF has also produced the same in frogs whose livers had been extirpated. In the so-called phloridzin diabetes the sugar is not formed from the glycogen of the liver, but to all appearances from the albumin (or proteids). The Bile and its Formation. By the employment of a biliary fistula, an operation which was first performed in 1844 by SCHWANN, it is possible to study the secretion of the bile. This secretion takes place at a very low pressure; therefore an apparently unimportant hindrance in the outflow of the bile, namely, a stoppage of mucus in the exit or the secretion of large quantities of viscous bile, may cause stagnation and absorption of the bile by means of the lymphatic vessels (ab. sorption icterus). The quantity of bile secreted during a specified time, say 24 hours, is rather difficult to determine with accuracy. The approxi- mate amount for the dog, as determined by BIDDER and SCHMIDT, is about 20 grms., with, in round numbers, 1 grm. of solids per kilo of the weight of the body. For human beings KANKE has calculated an average of 14 grms., with 0.44 grms. solids. The amount is de- pendent upon the nutrition. In fasting the quantity decreases, but increases after taking food. The statements are contradictory in 144 PHYSIOLOGICAL CHEMISTRY. regard to the time necessary after partaking of food before the secretion reaches its maximum. Formerly it was held that the secre- tion of bile is increased by food rich in albumin ; in later investi- gations, by ROSENBERG, it was found, on the contrary, that the fats give a greater stimulus to the secretion of bile than do the other nutritive substances. The drinking of water increases the secretion of bile. The statements of different investigators vary so much in regard to the action of different medicinal bodies in the secretion of bile that it is impossible to reach any conclusion on the subject. All investigators who have worked on this subject seem to agree that sodium salicylate is a true cathartic (RUTHERFORD, VIGNAL, LEWASCHEW, PREVOST and BINET, ROSENBERG). Also turpentine, which is a component of the so-called DURAND'S remedy, seems to increase the secretion (LEWASCHEW, PREVOST and BINET, ROSEN- BERG). Olive-oil is a very active cathartic (ROSENBERG). By the increased secretion of bile the amount of solids does not, as a rule, increase at the same rate as the water, and the concentration of the bile decreases. An exception to this is found only in the influence exerted by the bile itself, acting as it does as a powerful cathartic by which also the concentration of the secreted bile is increased. The bile is a mixture of the secretion of the liver-cells and the so-called mucus which is secreted by the glands of the biliary passages and by the mucous membrane of the gall-bladder. The secretion of the liver, which is generally poorer in solids than the bile from the gall-bladder, is thin and clear, while the bile collected in the gall-bladder is more ropy and viscous on account of the ab- sorption of water and the admixture of " mucus," and cloudy because of the admixture of cells, pigments, and the like. The specific gravity of the bile from the gall-bladder varies consider- ably, in man between 1.010 and 1.040. Its reaction is alkaline. The color changes in different animals: golden yellow, yellowish brown, olive-brown, brownish green, grass-green, or bluish green. Bile obtained from an executed person immediately after death is golden yellow or yellow with a shade of brown. Still cases occur in which fresh human bile has a green color. The ordinary post-mortem bile has a variable color. The bile of certain animals has a peculiar odor; as example, ox-bile has an odor of musk, espe- cially on warming. The taste of bile is also different in different THE LIVER. 145 animals. Human as well as ox bile has a bitter taste with a sweetish after-taste. The bile of the pig and rabbit has an intense persistent bitter taste. On heating bile to boiling it does not coagulate. It contains (in the ox) only traces of true mucin, and its ropy proper- ties depend, it seerns, chiefly on the presence of a nucleoalbumin similar to mucin (PAIJKULL). The specific constituents of the bile are bile-acids combined with alkalies, bile-pigments, and besides small quantities of lecithin, cholesterin, soaps, neutral fats, urea,&ud. mineral substances (sodium chloride, calcium and magnesium phos- phate, and iron). Bile-salts. All bile-acids can be divided into two groups, the glycocholic- and the taurocholic-acid groups. All glycocholic acids contain nitrogen, but are free from sulphur and can be split with the addition of water into glycocoll (amido-acetic acid) and an acid free from nitrogen, cholalic acid. All taurocholic acids con- tain nitrogen and sulphur and are split, with the addition of water, into taurin (amido-isethionic acid) containing sulphur and cholalic acid. The reason of the existence of different glycocholic and tau- rocholic acids depends on the fact that there are several cholalic acids. The different bile-acids occur in the bile as alkali salts, generally in combination with sodium, but in sea-fishes as potassium salts. In the bile of certain animals we find almost solely glycocholic acid, in others only taurocholic acid, and in other animals a mixture of both (see below). All alkali salts of the biliary acids are soluble in water and alco- hol, but insoluble in ether. Their solution in alcohol is therefore precipitated by ether, and this precipitate, with the proper care in manipulation, gives, for nearly all kinds of bile thus far investigated, rosettes or balls of fine needles or 4-6-sided prisms (PLATTNER'S crystallized bile). Fresh human bile also crystallizes readily. The bile-acids and their salts are optically active and dextro-rotary. The former are dissolved by concentrated sulphuric acid at the ordi- nary temperature, forming a reddish-yellow liquid which has a beautiful green fluorescence. On carefully warming with concen- trated sulphuric acid and a little cane-sugar, the bile-acids give a beautiful cherry-red or reddish-violet liquid. PETTENKOFER'S reac- tion for bile-acids is based on these facts. 146 PHYSIOLOGICAL CHEMISTRY. PETTENKOFER'S lest for bile-acids is performed as follows. A small quantity of bile in substance is dissolved in a small porcelain dish in concentrated sulphuric acid and warmed, or some of the liquid containing the bile-acids is mixed with concentrated sulphuric acid, taking special care in both- cases that the temper- ature does not rise higher than 60-70° C. Then a 10$ solution of cane-sugar is added, drop by drop, continually stirring with a glass rod. The presence of bile is indicated by the production of a beau- tiful red liquid^ whose color does not disappear at the ordinary temperature, but becomes more bluish violet in the course of a day. This red liquid shows a spectrum with two absorption-bands, the one at F and the other between D and E, near E. This extremely delicate test fails, however, when the solution is heated too high or if an improper quantity — generally too much — of the sugar is added. In the last-mentioned case the sugar easily carbonizes and the test becomes brown or dark brown. The reac- tion readily fails if the sulphuric acid contains sulphurous acid or the lower oxides of nitrogen. Many other substances, such as al- bumin, oleic acid, amylacohol, morphin, and others, give a similar reaction, and therefore in doubtful cases the spectroscopic exami- nation of the red solution must not be forgotten. PETTEJTKOFER'S test for the bile-acids depends essentially on the fact that furfurol is formed from the sugar by the sulphuric acid, and this body can therefore be substituted for the sugar in this test (MYLIUS). According to MYLIUS and v. UDRANSZKY a 1 p. m. solu- tion of furfurol should be used. Dissolve the bile, which must first be purified by animal charcoal, in alcohol. To each c. c. of the alcoholic solution of bile in a glass add 1 drop of the furfurol solu- tion and 1 c. c. cone, sulphuric acid, and cool when necessary so that the test does not become too warm. This reaction, when per- formed as described, will detect ^5— ?V milligram cholalic acid (v. UDRANSZKY). Other modifications of PETTEKKOFER'S test have been proposed. Glycocholic Acid. The constitution of that glycocholic acid occurring in human and ox bile, which has been most studied and which is identical with the cholic acid of STRECKER and GMELIN, is represented by the formula C^II^NOe. Glycocholic acid is absent or nearly so in the bile .of carnivora. On boiling with acids or THE LIVER. 147 alkalies this acid, which is analogous to hippuric acid, is converted into cholalic acid and glycocoll. Glycocholic acid crystallizes in fine, colorless needles or prisms. It is soluble with difficulty in water (in about 300 parts cold and 120 parts boiling water), and is easily precipitated from its alkali- salt solution by the addition of dilute mineral acids. It is readily soluble in strong alcohol, but with great difficulty in ether. The solutions have a bitter but at the same time sweetish taste. The salts of the alkalies and alkaline earths are soluble in alcohol and water. The salts of the heavy metals are mostly insoluble or soluble with difficulty in water. The solution of the alkali salts in water is precipitated by sugar of lead, copper-oxide and ferric salts, and silver nitrate. The preparation of pure glycocholic acid may be performed in several ways. We may precipitate the bile, which has been freed from mucus by means of alcohol and the alcohol removed by evaporation, by a solution of lead acetate. The precipitate is then decomposed by a soda solution and heat, evaporated to dryness, and the residue extracted with alcohol, which dissolves the alkali glycocholate. The alcohol is distilled from the filtered solution and the residue dissolved in water; this solution is now decolorized by animal charcoal, and the glycocholic acid precipitated from the solution by the addition of a dilute mineral acid. The acid may be obtained in crystals either from boiling water, on cooling, or from strong alcohol by the addition of ether. The reader is re- ferred to more exhaustive works for other methods of preparation. Hyo-glycocholic Acid, CaTl^sNOs, is the crystalline glycocholic acid obtained from the bile of the pig. It is very insoluble in water. The alkali salts, whose solutions have an intense bitter taste without any sweetish after-taste, are precipitated by CaCl2 , BaCla , and MgCl3 , and may be salted out like a soap by Na2SO4 when added in sufficient quantity. Besides this acid there occurs in the bile of the pig still another glycocholic acid (JOLIN). The glycocholate in the bile of the rodent is also precipitated by the above- mentioned salts, but cannot, like the corresponding salt in the human or ox bile, be precipitated on saturating with a neutral salt (Na3SO4). Guano bile- acid possibly belongs to the glycocholic-acid group, and is found in Peruvian guano but has not been thoroughly studied. Taurocholic Acid. This acid, which is found in the bile of man, carnivora, oxen and a few other herbivora, such as sheep and goats, and which is identical with the choleic of STRECKEB and DEMARCAY, has the constitution C^H^NSOy. On boiling with acids and alka- lies it splits into cholalic acid and taurin. 148 PHYSIOLOGICAL CHEMISTRY. Taurocholic acid may be obtained, though only with difficulty, in fine needles which deliquesce in the air (PARKE). It is very soluble in water, and can hold the difficultly-soluble glycocholic acid in solution. This is the reason why a mixture of glycocholate with a sufficient quantity of taurocholate, which often occurs in ox- bile, is not precipitated by a dilute acid. Taurocholic acid is readily soluble in alcohol but insoluble in ether. Its solutions have a bitter- sweet taste. Its salts are, as a rule, readily soluble in water, and the solutions of the alkali salts are not precipitated by copper sul- phate, silver nitrate, or sugar of lead. Basic lead acetate gives, on the contrary, a precipitate which is soluble in boiling alcohol. Taurocholic acid is best prepared from decolorized, crystallized dog-bile, which contains only taurocholate. The solution of this bile is precipitated by basic lead acetate and ammonia, and the washed precipitate dissolved in boiling alcohol. The filtrate is now treated with H2S, and this filtrate is evaporated at a gentle heat to a small volume, and treated with an excess of water-free ether. The acid sometimes partially crystallizes. Cheno-taurocholic Acid. This is the most essential acid of goose-bile and has the formula CagH^gNSOe. This acid, though little studied, is known to be amorphous and soluble in water and alcohol. As repeatedly mentioned above, the two bile-acids split on boiling with acids or alkalies into non-nitrogenized cholalic acid and glycocoll or taurin. Therefore we will now describe the prod- ucts of this splitting. Cholalic Acid. The ordinary cholalic acid obtained as a decom- position product of human and ox bile, which occurs regularly in the contents of the intestines and in the urine in icterus, and which is identical with DEMAR9AY's cholic acid, has, according to STRECKER and nearly all recent investigators, the constitution C^H^Og ; but others give as the formula C^H^Os (LATSCHINOFF). According to MYLIUS, cholalic acid is a monobasic alcohol-acid with a secondary and two primary alcohol groups. Its formula may there- ( CHOH fore be written C^H^ \ (CH2OH)2. On oxidation it first yields (COOH deliydrocholalic acid (AUTHOR), and then bilianic acid (CLEVE). The formulae of these acids (when we take C^ for the cholalic acid) are C^H^Oa and C^H^Og. On reduction (in putrefaction) cholalic acid may yield desoxy cholalic acid, C24H4004 (MYLIUS). THE LIVER. 149 Cholalic acid crystallizes partly with one molecule of water, in rhombic plates or prisms, and partly in larger rhombic tetrahedra or octahedra with 1 mol. of alcohol of crystallization (MYLIUS). These crystals become quickly opaque and porcelain- white in the air. They are quite insoluble in water (in 4000 parts cold and 750 parts boiling), rather soluble in alcohol, but soluble with difficulty in ether. The amorphous cholalic acid is less insoluble. The solutions have a sweetish-bitter taste. The crystals lose their alcohol of crystallization only after a lengthy heating to 100-120° C. The acid free from water and alcohol melts at -j- 195°C. The alkali salts are readily soluble in water, but when treated with a concentrated caustic or carbonated alkali solution may be separated as an oily mass which becomes crystalline on cooling. The alkali salts are not readily soluble in alcohol, and on the evaporation of the alcohol they may crystallize. The specific rotary power of the sodium salt is (<*)D = -f 31.4°. The watery solution of the alkali salts, when not too dilute, is precipitated immediately or after some time by sugar of lead or by barium chloride. The barium salt crystallizes in fine, silky needles, and it is rather insoluble in cold, but somewhat easily soluble in warm water. The barium salt, as well as the lead salt which is in- soluble in water, is soluble in warm alcohol. To prepare cholalic acid we boil ox-bile for 18-36 hours with strong caustic alkali, or, better, with as much barium hydrate as the boiling liquid will dissolve. During the boiling add when necessary more barium hydrate. The boiling-hot liquid is strained and concentrated until a crystalline mass separates in large quantity. This mass is separated from the mother-liquor and strongly pressed and recrystallized a few times from boiling water. The recrystal- lized barium salt is now dissolved, and the solution decomposed by hydrochloric acid. The acid which separates is dissolved in boiling alcohol, and the acid generally separates as crystals immediately on cooling. By recrystallization from ethyl alcohol and finally from methyl alcohol the acid may be readily obtained in pure white crys- tals the size of a pea. Choleic Acid, C^H^A, is another cholalic acid named by LATSCHI^OFF, which is obtained from ox-bile with ordinary cholalic acid, though in very, small amounts (hardly ^ the quan- tity of the latter). The barium salt of this cholalic acid is 150 PHYSIOLOGICAL CHEMISTRY. more readily soluble than the barium salt of the ordinary cholulic acid. Choleic acid yields on oxidation, first, deUydroclioleic acid, C^HggO*, and then cholanic acid, C^HggOg-f J H20 (LATSCHINOFF). Fellic Acid, C^H^O^, is a cholalic acid, so called by SCHOT- TEN, and which he obtained from human bile, along with the ordi- nary acid. This acid is crystalline, is insoluble in water, and yields barium and magnesium salts which are very insoluble. It does not give PETTENKOFER'S reaction easily and gives a more reddish- blue color. The hyo-glycocholic and the cheno-taurocholic as well as the glycochplic acid of the bile of rodents yield corresponding cholalic acids. On boiling cholalic acids with acids, by putrefaction in the intestines, or by heating, they lose water and are converted into an anhydride, the so-called dy sly sin. The dyslysin, €24113603, corre- sponding to ordinary cholalic acid, and which occurs in faeces, is amorphous, insoluble in water and alkalies. Choloidic acid is ^called the first anhydride, or an intermediate product in the forma- tion of dyslysin. According to HOPPE-SEYLER, choloidic acid is perhaps only a mixture of cholalic acid and dyslysin. On boiling dyslysin with caustic alkali it is reconverted into the corresponding cholalic acid. Glycocoll, C2H6N02 , or amido-acetic acid, NH2.CH2.COOH, also called glycine, or sugar of gelatine, has been found in the muscles of pecten irradians, but it is of special interest as a decomposi- tion product of certain protein substances — gelatine and spongin — as also of hippuric acid or glycocholic acid on splitting them by boiling with acids. Glycocoll forms colorless, often large, hard rhombic crystals or four-sided prisms. The crystals taste sweet and dissolve easily in cold (4.3 parts) water. They are insoluble in alcohol and ether; in warm spirits of wine they dissolve, but with difficulty. Glycocoll combines with acids and bases. Under the last-mentioned combi- nations we must mention those with copper and silver. Glycocoll dissolves copper hydroxide in alkaline liquids, but does not reduce it at the boiling temperature. A boiling-hot solution of glycocoll dissolves freshly-precipitated copper hydroxide, forming a blue liquid from which dark-blue needles crystallize on cooling, if THE LIVER. 151 the liquid is sufficiently concentrated. The combination of gly- cocoll with HC1 is soluble in water and alcohol. Glycocoll is best prepared from hippuric acid by coiling it 10- 12 hours with 4 parts of dilute sulphuric acid, 1 : 6. After cooling separate the beuzoic acid, concentrate the filtrate, remove the re- mainder of the benzoic acid by shaking with ether, remove the sul- phuric acid by BaC03, and evaporate the filtrate to crystallization. Taurin, C2H7NS03, or amido-ethylsulphonic acid, NH2.C21I4. SOaOH. This body is well known as a splitting product of taurocholic acid, and may occur in small quantities 'in the contents of the intestines. It has also been found in the lungs and kidneys of oxen and in the blood and muscles of cold-blooded animals. Taurin crystallizes in colorless, often in large, shining, 4-6 sided prisms. It dissolves in 15-16 parts of water at ordinary tempera- tures, but rather more easily in warm water. It is insoluble in abso- lute alcohol and ether; in cold spirits of wine it dissolves slightly, but more when warm. Taurin yields acetic and sulphurous acids, but no alkali sulphides, on boiling with strong caustic alkali. The amount of sulphur can be determined as sulphuric acid after fusing with saltpetre and soda. Taurin combines with metallic ox- ides. The combination with mercuric oxide is white, insoluble, and is formed when a solution of taurin is boiled with freshly-pre- cipitated mercuric oxide (J. LANG). This combination may be used in detecting the presence of taurin. Tauriu is not precipi- tated by metallic salts. The preparation of taurin from bile is very simple. The bile is boiled a few hours with hydrochloric acid. The filtrate from the dyslysin and choloidic acid is concentrated well in the water-bath, and filtered so as to remove the common salt and other substances which have separated. Then evaporate to dryness, and treat the residue with strong alcohol, which dissolves the hydrochlorate of glycocoll, while the taurin remains. (The alcoholic solution of hydrochlorate of glycocoll may be used in the preparation of gly- cocoli by evaporating the alcohol and dissolving the residue m water, decomposing the solution with lead hydroxide, filtering,, and freeing the solution from lead by H,S, and strongly concen- trating this filtrate. The crystals which separate are dissolved and decolorized by animal charcoal, and the solution evaporated to crys- tallization.) The above-obtained residue containing the taurin is dissolved in as little water as possible, filtered warm, and treated 152 PHYSIOLOGICAL CHEMISTRY. with an excess of alcohol. The crystalline precipitate which, im- mediately forms is filtered as soon as possible, and the taurin now separates, on cooling, into very long needles or prisms. These crystals may be purified by recrystallization from a little warm water. Though the taurin shows no positive reactions, it is chiefly identified by its crystalline form, by its solubility in water and in- solubility in alcohol, by its combination with mercuric oxide, by its non-precipitability by metallic salts, and above all by its containing sulphur. THE DETECTION OF BILE-ACIDS IN ANIMAL FLUIDS. To obtain the bile-acids pure so that PETTENKOFER'S test can be ap- plied to them, the albumins and fat must first be removed. The albumin is removed by making the liquid first neutral and then adding a great excess of alcohol, so that the mixture contains at least 85 vols. per cent of water-free alcohol. Now filter, extract' the pre- cipitated albumin with fresh alcohol, unite all filtrates, distil the alcohol, and evaporate to dryness. The residue is completely ex- hausted with strong alcohol, filtered, and the alcohol entirely evapo- rated from the filtrate. The new residue is dissolved in water, and filtered if necessary, and the solution precipitated by basic lead acetate and ammonia. The washed precipitate is dissolved in boiling alcohol, filtered while warm, and a few drops of soda solution added. Then evaporate to dryness, extract the residue with absolute alcohol, filter, and add an excess of ether. The precipitate now formed may be used for PETTENKOFER'S test. It is not necessary to wait for a crystallization ; but one must not consider the crystals which form in the liquid as being positively-crystallized bile. It is also possible for needles of alkali acetate to'be formed. For the detection of bile- acids in urine see Chapter XIV. Bile-pigments. The bile-coloring matters known thus far are relatively numerous, and in all probability there are still more. Most of the known bile-pigments are not found in the normal bile, but occur either in post-mortem bile or, principally, in the bile con- crements. The pigments which occur under physiological condi- tions are the reddish-yellow bilirubin, the green biliverdin, and some- times there is also observed in fresh human bile a pigment closely related to hydrobilirubin. The pigments found in gall-stones are (besides the bilirubin and biliverdin) bilifuscin, biliprasin, bilihumin, bilicyanin (and choletelin ?). Besides these, others have been observed in human and animal bile. The two above-mentioned physiological pigments, bilirubin and biliverdin, are those which serve to give the golden-yellow or orange-yellow or sometimes THE LIVER. 153 greenish color to the bile, or when, as is most frequently the case in ox-bile, the two pigments are present in the bile at the same time, producing the different shades between reddish brown and green. Bilirubin. This pigment, according to the common accepta- tion, has the formula C16H18N203 (MALT) and is designated by the names CHOLEPYRRHI^, BILIPHJEIN, BILIFULVIN and H^EMATOIDIN. It occurs chiefly in the gall-stones as bilirubin-calcium. It is further found in the bile, especially in man and carnivora ; sometimes, however, the latter when fasting or in a starving condition may have a green bile in the gall-bladder. It occurs also in the contents of the small intestines, in blood-serum of the horse, in old blood extravasations (as haematoidin), and in the urine and the yellow- colored tissue in icterus. The bilirubin is in all probability a forma- tion from the haematin which it closely resembles. It is converted into hydrobilirubin, Cg.}!!^^ (MALY), by hydrogen in a nascent state. It is claimed by several investigators to be identical with the urinary pigment urobilin, as well as with stercobilin (MASIUS and VANLAIR), which is found in the contents of the intestines. There is no doubt that a great similarity exists between these pigments, but their identity is emphatically denied by MAcMuNN. On oxida- tion bilirubin yields biliverdin and other coloring matters (see below). Bilirubin is partly amorphous and partly crystalline. The amorphous bilirubin is a reddish-yellow powder of nearly the same color as amorphous antimony sulphide; the crystalline bilirubin has nearly the same color as crystallized chromic acid. The crystals, which can easily be obtained by allowing a solution of bilirubin in chloroform to spontaneously evaporate, are reddish-yellow, rhombic plates, whose obtuse angles are often rounded. Bilirubin is insoluble in water, slightly soluble in ether, some- what more soluble in alcohol, easily soluble in chloroform, especial- ly in the warmth, and less soluble in benzol, carbon disulphide, amyl alcohol, fatty oils, and glycerin. Its solutions show no absorption- bands, but only a continuous absorption from the red to the violet end of the spectrum, and they have, even on diluting greatly, (1 : 500000) a decided green color. The combinations of bilirubin with alkalies are insoluble in chloroform, and bilirubin may be separated from its solution in chloroform by shaking with dilute caustic alkali (differing from lutein). Solutions of bilirubin-alkali 154 PHYSIOLOGICAL CHEMISTRY. in water are precipitated by the soluble salts of the alkaline earths and also by metallic salts. If an alkaline solution of bilirubin be allowed to stand in contact with the air, it gradually absorbs oxygen and green biliverdin is formed. Biliverdin is also formed from bilirubin by 'oxidation under other conditions. A green coloring matter similar in appearance is formed by the action of other reagents such as 01, Br, and I. In these cases it does not seem to be biliverdin, but a substitution product of bilirubin (TnuDiCHUM, MALY) which is obtained. GMELIN'S Reaction for Bile-pigments. If we carefully pour under a solution of bilirubin-alkali in water nitric acid containing some nitrous acid, we obtain a series of colored layers at the juncture of the two liquids, in the following order from above downwards: green, blue, violet, red, and reddish yellow. This color reaction, GMELIN'S test, is very delicate and serves to detect the presence of one part bilirubin in 80,000 parts liquid. The green ring must never be absent; and also the reddish violet must be present at the same time, otherwise the reaction may be confused with that for lutein, which gives a blue or greenish ring. The nitric acid must not contain too much nitrous acid, for then the reaction takes place too quickly and it does not become typical. Alcohol must not be present in the liquid, because, as is well known, it gives a play of colors, in green or blue, with the acid. HUPPERT'S Reaction. If a solution of bilirubin-alkali is treated with milk of lime or with calcium chloride and ammonia, a precipi- tate is produced consisting of bilirubin-calcium. If this moist pre- cipitate, which has been washed with water, is placed in a test- tube and the tube half filled with alcohol which has been acidified with sulphuric acid, and heated to boiling for some time, the liquid becomes emerald -green or bluish green in color. This reaction is a good and easily-performed test for bile-pigments. In regard to the modifications of GMELIN'S test and certain other reactions for bile-pigments, see Chapter XIV (Urine). That the characteristic play of colors in GMELIN'S test is the result of an oxidation is generally admitted. The first oxidation step is the green biliverdin. Then follows a blue coloring matter which HEINSIUS and CAMPBELL call bilicyanin and STOKVIS calls cliolecyanin, and which shows a characteristic absorption-spectrum. TI1K LI V Eli. ir>f; The neutral solutions of these coloring matters are, according to STOKVIS, bluish green or steel-blue with a beautiful blue fluores- cence. The alkaline solutions are green and have no marked fluorescence. The neutral and alkaline solutions show three ab- sorption-bands, one sharp and dark in the red between G and D, nearer to (7; a second, less defined, covering D\ and a third, form- ing only a faint shadow, in the green, exactly in the middle, between D and E. The strongly-acid solutions are violet-blue and show two bands, described by JAFEE, between the lines G and E, separated from each other by a narrow space near D. The next oxidation step after these blue coloring matters gives a red pig- ment, and lastly a yellowish-brown pigment, called choletelin by MALY, which shows no absorption-spectrum. Bilirubin is best prepared from gall-stones of oxen, these con- cretions being very rich in bilirubin-calcium. The finely-powdered concrement is first exhausted with ether and then with boiling water, so as to remove the cholesterin and bile-acids. The powder is then treated with hydrochloric acid, which sets free the pigment. Wash thoroughly with water and alcohol, dry, and extract continuously with boiling chloroform. After distilling the chloroform from the solution, treat the powdered residue with absolute alcohol to remove the bilifuscin ; dissolve the remaining bilirubin in a little chloroform ; precipitate it from this solution by alcohol, and do this several times if necessary. The bilirubin is finally dissolved in boiling chloroform and allowed to crystallize on cooling. The quantitative estimation of bilirubin may be made by the spectro-photometrical method, according to the steps suggested for the blood-coloring matters, Biliverdin, 08H9N02. This body, which is formed by the oxida- tion of bilirubin, occurs in the bile of many animals, in vomited matter, in the placenta of the bitch (?), in the shells of birds' eggs, in the urine in icterus, and sometimes in gall-stones, although in very small quantities. Biliverdin is amorphous, or at least it has not been obtained in well-defined crystals. It is insoluble in water, ether, and chloro- form (this is true at least for the artificially-prepared biliverdin, while the green pigment of ox-bile is soluble in chloroform, accord- ing to MACMUNN), but is soluble in alcohol or glacial acetic acid, showing a beautiful green color. It is dissolved by alkalies giving a brownish-green color, and this solution is precipitated by 156 PHYSIOLOGICAL CHEMISTRY. acids, as well as by calcium, barium, and lead-salts. Biliverdin gives HUPPERT'S and GMELLN^S reactions, commencing with the blue color. It is converted into hydrobilirubin by nascent hydro- gen. On allowing the green bile to stand, also by the action of ammonium sulphide, the biliverdin may be reduced to bilirubin (HAYCEAFT and SCOFIELD). Biliverdin is most simply prepared by allowing a thin layer of an alkaline solution of bilirubin to stand exposed to the air in a dish until the color is brownish green. The solution is then pre- cipitated by hydrochloric acid, the precipitate washed with water until no HC1 reaction is obtained, then dissolved in alcohol and the pigment again separated by the addition of water. Any biliru- bin present may be removed by means of chloroform. •• tr>ri Bilifuscin, so named by STADELER, is an amorphous brown pigment, soluble in alcohol and alkalies, nearly insoluble in water and ether, and soluble with great difficulty in chloroform (when bilirubin is not present at the same time). It is found in post-mortem bile and gall-stones. Biliprasin is a green pigment prepared by STADELER from gall-stones, which perhaps is only a mixture of biliverdin and bilirubin. Bilihumin is the name given by STADELEK to that brownish amorphous residue which is left after extracting gall-stones with chloroform, alcohol, and ether. It does not give GMELIN'S test. Bilicyanin is also found in human gall-stones (HEINSIUS and CAMP- BELL). Cholo Iwmatin, so called by MACMUNN, is a pigment often occurring in sheep- and ox-bile and characterized by four absorption- bands, and which is formed from haematin by the action of sodium amalgam. In the dried con- dition, obtained by the evaporation of the chloroform solution, it is green, and in alcoholic solution olive-brown. GMELIN'S and HUPPERT'S reactions are generally used to detect the presence of bile-pigments in animal fluids or tissues. The first, as a rule, can be performed directly, and the presence of albumin does not interfere with it, but, on the contrary, it brings out the play of colors more strikingly. If blood-coloring matters are present at the same time, the bile-coloring matters are first precipitated by the addition of sodium phosphate and milk of lime. This precipi- tate containing the bile-pigments may be used directly in HUPPERT'S reaction, or may be treated with water and some hydrochloric acid, and then shaken with chloroform free from alcohol, and this chloroform solution used in testing for the bile-pigments. Besides the bile-acids and bile-pigments we also have in the bile cholesterin, lecithin, palmitin, stearin, olein, and soaps of the corresponding fatty acids. In some animals the bile contains a diastatic enzyme. Cliolin and glycero- phosphoric acid, when they are present, may be considered as decomposition products of lecithin. Urea occurs, though only as traces, as a physiological constituent of human, ox, and dog bile. The mineral constituents THE LIVER. 167 of the bile are, besides the alkalies, to which the bile acids are united, sodium and potassium chloride, calcium and magnesium phosphate, and iron — 0.04-0.11 p. m. in human bile, chiefly com- bined with phosphoric acid (YOUNG). Traces of copper are habitu* ally present, and traces of zinc are often found. Sulphates are entirely absent or only occur in very small amounts. Quantitative Composition of the Bile. Complete analyses of human bile have been made by HOPPE-SEYLER and his pupils. The bile was removed as fresh as possible from the gall-bladder of cadavers whose livers showed no remarkable change. The follow- ing figures of SOCOLOFF are the average of six analyses, and those of HOPPE-SEYLER of five analyses. The relationship between the glycocholate and taurocholate was found by fusing the precipitate consisting of biliary alkalies obtained by ether from the alcoholic extract with saltpetre and soda. On determining the amount of sulphur in the fused mass the taurocholic acid can be calculated from this. 100 parts BaS04 correspond to 220. 86 parts taurocholic acid. The figures are parts per 1000. TRIFANOWSKI. SOCOLOFF. HOPPE-SEYLER. I. II. Mucin 24.8 13.0 Remaining bodies iusol. m alcohol. . 4.5 14.6 Taurocholate 7.5 19.2 Glycocholate 21.0 44 Soaps 8.1 16.3 Cholesteriu 25 3.3 Lecithin ) * o 0.2 Fat f 53 3.6 Ferric phosphate 37.20 15.67 4904 14.60 12.9 1.4 8.7 30.3 13.9 3.5 3.3 7.3 0.166 Older and less complete analyses of human bile have been made by FRERICHS and v. GORUP-BESANEZ. The bile analyzed by them was from perfectly healthy persons who had been executed or acci- dentally killed. The two analyses of FRERICHS are, respectively, of (I) an 18-year-old and (II) a 32-year-old male. The analyses of v. GORUP-BESANEZ are of (I) a man of 49 and (II) a woman of 29. The results are, as usual, in parts per 1000. FRERICHS. V. GORUP-BKSANKZ. I. Water 860.0 Solids 140.0 Biliarysalts 72.2 Mucus and pigments 26.6 Cholesterin 1-6 Fat 3.2 Inorganic substances 6.5 II. 859.2 140.8 91.4 29.8 2.6 ) 9.2 | 7.7 822.7 177.3 107.9 22.1 47.8 10.8 II. 898.1 101.9 56.5 14.5 30.9 6.3 158 PHYSIOLOGICAL CHEMISTRY. The bile of the gall-bladder is, as above stated, richer in solids than the bile of the liver. Human bile obtained by means of a fistula contained 22.4-22.8 p. m. solids, according to JACOBSEN, who determined the mineral substances in the same bile and found the following, calculated from the dry residue: KC1 12.85, NaCl 245.1, Na3P04 59.8, Ca3(P04)2 16.7, and Na2C03 41.8 p. m. The relationship between the amounts of glycocholates and taurocholates in the human bile seems to be quite variable. According to the analyses and observations of the majority of in- vestigators, the human bile is, in most cases, relatively richer in glycocholic acid and correspondingly poor in taurocholic acid. In animals the relative proportion of the two acids varies very much. It has been found, on determining the amount of sulphur, that, so far as the experiments have gone, taurocholic acid is the prevailing acid in carnivorous mammalia, birds, snakes, and fishes. Among the herbivora sheep and goats have a predominance of taurocholic acid in the bile. Ox-bile sometimes contains tauro- cholic acid in excess, in other cases glycocholic acid predominates, and in a few cases the latter occurs almost alone. The bile of the rabbit, hare, and kangaroo contains, like the bile of the pig, almost exclusively glycocholic acid. A distinct influence on the relative amounts of the two bile-acids by different foods has not been de- tected. HITTER claims to have found a decrease in the quantity of taurocholic acid in calves when they pass from the milk to the plant diet. The gases of the bile consist of a large quantity of carbon dioxide, which increases with the amount of alkalies, only traces of oxygen, and a very small quantity of nitrogen. . Little is known in regard to the properties of the bile in disease. The quan- tity of urea is found to be considerably increased in uraemia. Leucin and tyrosin are observed in acute yellow atrophy of the liver and in typhus. Traces of albumin (without regard to nucleoalbumin) have several times been found in the human bile. The so-called pigmentary acholia, or the secretion of a bile containing bile-acids but no bile-pigments, has also been repeatedly noticed. In all such cases observed by HITTER he found a fatty degeneration of the liver-cells, in return for which, even in excessive fat infiltration, a normal bile containing pigments was secreted. The secre- tion of a bile nearly free from bile-acids has been observed by HOPPE-SEYLER in amylaceous degeneration of the liver, and also by K. MORNER. A number of substances, such as turpentine, salicylic acid, potassium bromide and iodide, arsenic, iron, lead, and mercury (PREVOST and BINET), are eliminated by the bile. In animals, dogs, and especially rabbits it has been observed THE LIVER. 159 that the blood-coloring matters pass into the bile in poisoning and in other cases, causing a destruction of the blood -corpuscles (WERTHEIMER and -M.EYER, s ILEHNE). Chemical Formation of the Bile. The first question to be answered is the following : Do the specific constituents of the bile, the bile-acids and bile-pigments, originate in the liver; and if this is the case, do they come from this organ only, or are they also formed elsewhere? The investigations of the blood, and especially the comparative investigations of the blood of the portal and hepatic veins under normal conditions, have not given any answer to this question. To decide this, therefore, it is necessary to extirpate the liver of animals or isolate it from the circulation. If the bile constituents are not formed in the liver or at least not alone in this organ, but only eliminated from the blood, then, after the extirpation or re- moval of the liver from the circulation, an accumulation of the bik constituents is to be expected in the blood and tissues. If the bile- constituents, on the contrary, are formed exclusively in the liver, then the above operation naturally would give no such result. It the choledochus duct is tied, then the bile constituents will be collected in the blood or tissues whether they are formed in the liver or elsewhere. From these principles KEENER has tried to demonstrate by ex- periments on frogs that the bile-acids are produced exclusively in the liver. While he was unable to detect any bile-acids in the blood and tissues of these animals after extirpation of the liver, still hs was able to discover them on tying the choledochus duct. The investigations of LUDWIG and FLEISCHL show that in the dog the bile-acids originate in the liver alone. After tying the chole- dochus duct they observed that the bile constituents were absorbed by the lymphatic vessels and passed into the blood through the thoracic duct. - Bile-acids could be detected in the blood after such an operation, while they could not be detected in the normal blood. But when the choledochus and thoracic ducts were both tied at the same time, then not the least trace of bile-acids could be detected in the blood, while if they are also formed in other organs and tissues they should have been present. The general opinion is that the bile-acids are formed onlv in the liver; still there are investigators 160 PHYSIOLOGICAL CHEMISTRY. who have other views. BALDI claims that the formation of bile- acids does not take place only in the liver, but in the entire organism, and he claims to have detected bile-acids in the normal, circulating blood of different organs. It has been indubitably proved that the bile-pigments may be formed in other organs besides the liver, for, as is generally ad- mitted, the coloring matter haematoidin, which occurs in old blood extravasations, is identical with the bile-pigment bilirubin (see page 80). LATSCHENBERGER has also observed in horses, under physio- logical conditions, a formation of bile-pigments from the blood- coloring matters in the tissues. Also the occurrence of bile- pigments in the placenta seems to depend on their formation in that organ, while the occurrence of small quantities of bile-pigments in the blood-serum of certain animals probably depends on an ab- sorption of the same. Though the bile-pigments may be formed in other organs, it still seems that their formation under physiological conditions occurs mainly in the liver. By experimenting on pigeons STERN was able to detect bile-pigments in the blood-serum five hours after tying the biliary passages alone, while after tying all the vessels of the liver and also the biliary passages no bile-pigments could be detected either in the blood or the tissues of the animal, which was killed 10-12 hours after the operation. MINKOWSKI and NAUNYN have also found that poisoning with arseniuretted hydrogen pro- duces a liberal formation of bile-pigments and the secretion, after a short time, of a urine rich in biliverdin in previously healthy geese. In geese with extirpated livers this does not occur. The great im- portance of the liver in the formation of bile-pigments seems to be settled, even though these bodies may be formed also in other organs. In regard to the materials from which the bile-acids are pro- duced, it may be said with certainty that the two components, glycocoll and taurin, which are both nitrogenized, are formed from the protein bodies. In regard to the origin of the non-nitro- genized cholalic acid, which was formerly considered as originat- ing from the fats, we know nothing positively. The blood -coloring matters are considered as the mother- substance of the bile-pigments. If the identity of hsematoidin and bilirubin were beyond doubt, then this view might be consid- THE LIVER. 161 ered as proved. Independently, however, of this identity, which is not admitted by all investigators, the view that the bile-pigments are derived from the blood -coloring matters has strong arguments in its favor. It has been shown by several experimenters (lately by LATSCHENBERGER) that a yellow or yellowish-red coloring matter can be formed from the blood-coloring matters which gives GMELIN'S test, and which, though it may not form a complete bile-pigment, is at least a step in its formation (LATSCHENBERGER). A further proof of the formation of the bile-pigments from the blood-color- ing matters consists in the fact that haematin yields urobilin, which is identical with hydrobilirubin, on reduction (HoFPE-SEYLER). Other investigators (NENCKI and SIEBER and LE NOBEL) claim that the substance thus obtained is not true urobilin, but, all things considered, it seems to be very nearly related. But even though the identity of urobilin with the hydro- bilirubin obtained by the reduction of bilirubin is disputed by certain investigators (MAcMuNK), still the substances may be so closely related that the relationship will serve as a proof of the for- mation of bilirubin from the blood-coloring matters. Further, hsematoporphyrin (see page 78) and bilirubin are isomers, accord- ing to NENCKI and SIEBER, and nearly allied. The formation of bilirubin from the blood-coloring matters is shown, according to the observations of several investigators, by the appearance of free haemoglobin in the plasma — by the destruction of the red corpuscles by widely-differing influences (see below) or by the in- jection of haemoglobin solution — causing an increased formation of bile-pigments. The amount of pigments in the bile is not only considerably increased (TARCHANOFF), but the bile-pigments may even pass into the urine under certain circumstances (icterus). After the injection of haemoglobin solution into a dog either sub- cutaneously or in the peritoneal cavity, GORODECKI observed in the secretion' of pigments by the bile an increase of 60# which lasted for twenty hours. If, then, iron-free bilirubin is derived from the haematin con- taining iron, then iron must be split off. This process may be represented by the following formula, according to NENCKI SIEBER, 04Fe + 2H20 - Fe = 2CWH18N,0,, 162 PHYSIOLOGICAL CHEMISTRY. though in reality it is probably more complicated. The question in what form or combination the iron is split off is of special inter- est, and also whether it is eliminated by the bile. This latter does not seem to be the case. In 100 parts of bilirubin which are elimi- nated by the bile there are only 1.4-1.5 parts iron, according to KUNKEL ; while 100 parts haematin contain about 9 parts iron. MINKOWSKI and BASERIN have also found that the abundant formation of bile-pigments occurring in poisoning by arseniuretted hydrogen does not increase the quantity of iron in the bile. The quantity apparently does not correspond with that in the decom- posed blood-coloring matters. On the contrary, it seems as if the iron, at least for a time, is retained by the liver as a pigment rich in iron. Such a pigment containing iron, which was formed by the decomposition of haemoglobin, was observed by NAUNYN and MINKOWSKI in the livers of birds, in arseniuretted hydrogen icterus. LATSCHEN- BERGER claims that a yellow or yellowish red pigment, " choleglobin" is derived from the blood-coloring matters, and acts as a step in the formation of the bile-pigments; and besides this he mentions an- other body consisting of dark grains and containing iron, which he designates as melanin. NEUMANN has observed in blood extrav- asations, besides hsematoidin, a pigment containing iron, for which he has proposed the name lic&matosiderin. An absorption of bile from the liver by the lymphatic vessels and the passage of the bile-constituents into the blood and urine occurs in retarded discharge of the bile, and usually in the differ- ent forms of hepatogenic icterus. But bile-pigments may also pass into the urine under other circumstances, especially in animals where a solution or destruction of the red blood-corpuscles takes place through injection of water or a solution of biliary salts, through poisoning by ether, chloroform, arseniuretted hydrogen, or toluylen- diamin; and in other cases. This occurs also in man in grave infectious diseases. We have therefore a second form of icterus, in which the blood-coloring matters are transformed into bile- pigments elsewhere than in the liver, blood, and tissues — a hcematogenic, chemical, or arihepatogenic icterus. Only bile-pig- ments occur in the urine in these cases, while in hepatogenic icterus the urine contains the bile-pigments and bile-acids at the THE LIVER. 163 same time (LEYDEN). This distinction, however, cannot be main- tained. It is certainly true that the presence of more than traces of bile-acids in the urine indicates the existence of hepatogenic icterus; but cases of absorption icterus undoubtedly occur in which no bile-acids can be detected in the urine. It has been clearly demonstrated by the above-mentioned obser- vations of MI^KOWSKI and NAUNYN on geese with extirpated livers that the bile-pigments are formed in the liver in arseni- uretted hydrogen icterus. It has also been demonstrated by STADEL- MANN and AFAXASSIEW that the icterus, after poisoning by arseni- uretted hydrogen or by toluylendiamin, must be considered as an absorption icterus. Perhaps the icterus depends in these cases upon the fact that the viscosity of the abundantly-secreted bile may form a check to its outflow, which counteracts the low secretion pressure, so that a stowing and absorption occurs (polycholic icterus). It is also possible that other cases of so-called haematogenic icterus may be of an analogous kind, and that every icterus is consequently hepatogenic. The occurrence of haematogenic icterus cannot be considered as proved beyond doubt, and it is questioned by several recent investigators. Appendix to the Bile. Bile Concretions. The concrements which occur in the gall-bladder vary consider- ably in size, form, and number, and are of three kinds, depending upon the kind and nature of the bodies forming their chief mass. One group of gall-stones contains lime-pigment as chief constituent, the other cholesterin, and the third calcium carbonate and phos- phate. The concrements of the last-mentioned group occur very seldom in man. The so-called cholesterin stones are those which occur most frequently in man, while the lime-pigment stones are not found very often in man, but often in oxen. The pigment-stones are generally not large in man, but in oxen and pigs they are sometimes found the size of a walnut or even larger. In most cases they consist chiefly of bilirubin-calcium with little or no biliverdin. Sometimes also small black or greenish black, metallic-looking stones are found, which consist chiefly of bilifuscin along with biliverdin. Iron and copper seem to be reg- 164 PHYSIOLOGICAL CHEMISTRY. ular constituents of pigment-stones. Manganese and zinc have also been found a few times. The pigment-stones are generally heavier than water. The cholesterin-stones, whose size, form, color, and structure may vary greatly, are often lighter than water. The fractured sur- face is radiated, crystalline, and frequently shows crystalline, con- centric layers. The cleavage fracture is waxy in appearance, and the fractured surface when rubbed by the nail also becomes like wax. By rubbing against each other in the gall-bladder they often become faceted or take other remarkable shapes. Their surface is sometimes nearly white and waxlike, but generally their color is variable. The quantity of cholestrin in the stones varies from 642 to 981 p. m. (RITTEE). The cholesterin-stones also sometimes con- tain variable amounts of lime-pigments which give them a very changeable appearance. Cholesterin, C^H^O, or, according to REINITZER, C27H470. Cholesterin is generally considered as a monatomic alcohol of the formula C^H^.OH. It yields a colored hydrocarbon (cholesteriline or cliolesterone), of the formula C^H^, with sulphuric acid, and this hydrocarbon is claimed by WEYL to be nearly related to the terpene group It has also been claimed that it is closely allied to cholalic acid. Cholesterin occurs in small amounts in nearly all animal fluids and juices. It occurs only rarely in the urine, and then in very small quantities. It is also found in the different tissues and organs — especially abundant in the brain and the nervous system, — further in the yolk of the egg, in semen, and in wool-fat. It appears (to- gether with isocholesterin) in the contents of the intestines, in excrements, and in the meconium. It occurs pathologically espe- cially in gall-stones, as well as in atheromatous cysts, in pus, in tu- berculous masses, old transudations, cystin fluids, excrements, and tumors. Several kinds of cholesterih seem to occur in the plant world Cholesterin which crystallizes from warm alcohol on cooling, and that which is present in old transudations, contains 1 mol. of water of crystallization, melts at 137° C., and forms colorless, trans- parent plates whose sides and angles frequently appear broken and whose acute angle is often 76° 30' or 87° 30'. In large quantities THE LIVER. 165 it appears as a mass of white plates which shine like mother-of- pearl and have a greasy feel. Cholesterin is insoluble in water, dilute acids and alkalies. It is neither dissolved nor changed by boiling caustic alkali. It is easily soluble in boiling alcohol and crystallizes on cooling. It dissolves readily in ether, chloroform, and benzol, and also in the volatile or fatty oils. It is dissolved to a slight extent by alkali salts of the bile, acids. For the detection of cholesterin we make use of its action with concentrated sulphuric acid, which, as above stated, gives a •colored hydrocarbon with this acid. If a mixture of five parts sulphuric acid and one part water acts on a cholesterin crystal, this crystal will show colored rings, first a bright carmine-red and then violet. This fact is made use of in the microscopic detection of cholesterin. Another test, and one very good for the microscopical detection of cholesterin, consists in treat- ing the crystal first with the above dilute acid and then with some iodine solution. The crystals will be gradually colored violet, bluish green, and a beautiful blue. SALKOWSKI'S Reaction. The cholesterin is dissolved in chloro- form and then treated with an equal volume of concentrated sulphuric acid. The cholesterin solution becomes first bluish red, then gradually more violet-red, while the sulphuric acid ap- pears dark red with a greenish fluorescence. If the chloroform solution is poured into a porcelain dish it becomes violet, then green, and finally yellow. SCHIFF'S Reaction. If a little cholesterin is placed in a porcelain dish with the addition of a few drops of a mixture of two to three vols. coiic. hydrochloric acid or sulphuric acid and one vol. of a medium solution of ferric chloride, and carefully evaporated to dryness over a small flame, a red- dish-violet residue is first obtained and then a bluish violet. If a small quantity of cholesterin is evaporated to dryness with a drop of concentrated nitric acid, we obtain a yellow spot which becomes deep orange- red with ammonia or caustic soda (not a characteristic reaction). Isocholesterin. This body, so called by SCHULTZE, is isomeric with the ordinary cholesterin and occurs in wool-fat, and is therefore found in abun- dant quantities in so-called lanolin. It does not give SALKOWSKI'S or SCHIFF'S reactions. We make use of the so-called cholesterin-stones in the prepara- tion of cholesterin. The powder is first boiled with water and then repeatedly boiled with alcohol. The cholesterin which on cooling separates from the warm filtered solution is boiled with 166 PHYSIOLOGICAL CHEMISTRY. a solution of caustic potash in alcohol so as to saponify any fat. After the evaporation of the alcohol we extract the cholesterin from the residue with ether, by which the soaps are not dissolved, filter, evaporate the ether, and purify the cholesterin by recrystallization from alcohol-ether. The cholesterin may be extracted from tissues and fluids by first extracting with ether and then purifying as above. It is detected and determined quantitatively in tissue, etc., by this same method. It is ordinarily easily detected in transudations and pathological formations by means of the microscope. CHAPTER VII. DIGESTION. THE purpose of the digestion is to separate those constituents of the food which serve as the nutriment of the body from those which constitute the waste, and to separate each in such a form that it may be easily taken up by the blood from the alimentary canal and conveyed to its proper organism. This demands not only mechanical but also chemical action. The first action which essentially depends on the physical properties of the food consists in a tearing, cutting, crushing, or grinding of the food, and serves chiefly to convert the nutritive bodies into a soluble and easily- absorbed form, or in the splitting of the same into simpler combi- nations for use in the animal synthesis. The solution of the nutritive bodies may take place in certain cases by the aid of water alone, but in most cases a chemical metamorphosis or splitting is necessary, and is effected by means of the acids or alkalies, or by the fluids secreted by the glands. The study of the processes of digestion from a chemical standpoint must therefore begin with the digestive fluids, their qualitative and quantitative composition, as well as their action on the nutriments and foods. I. The Salivary Glands and the Saliva. The salivary glands are partly albuminous- glands (as the parotid in man and mammalia and the submaxillary in rabbits), partly mucous-glands (as some of the small glands in the buccal cavity and the sublingual and submaxillary glands of many animals), and partly mixed glands (as the submaxillary gland in man). The alveoli of the albumin glands contain cells which are rich in albumin, but contain no mucin. The alveoli of thejnucin -glands U1U7BRSIT7 168 PHYSIOLOGICAL CHEMISTRY. contain cells rich in mucin but poor in albumin. A cell rich in albumin may also occur in the submaxillary and sublingual glands between the mucus-cells and the membrana propria, which in a few cases akes the form of a half-moon (lunula, according to GIANUZZI), and in other cases the cells rich in mucin are sur- rounded as by a ring, and sometimes certain alveoli may be com pletely filled. By continuous secretion the mucin-cells seem to give up all their mucin (EwALD, STOHR), so that only albumin-cells are to be seen (HEIDEJSTHAIN). During rest the mucin-cells are re-formed. According to the analyses of OIDTMAN, the salivary glands of a dog contain 790 p. m. water, 200 p. m. organic and 10 p. m. inorganic solids. Among the solids, in addition to albumin, we find, nucleoalbumin and mucin, diastatic enzyme, in certain animals nuclein, extractive bodies, leucin, traces of xanthin bodies, and mineral substances. The saliva is a mixture of the secretion of the above-mentioned groups of glands; therefore it is proper that we first study each of the different secretions by itself, and then the mixed saliva. The submaxillary saliva in man may be easily collected by introducing a canula into the Wharton's duct. The submaxillary saliva has not always the same composition or properties; these depend essentially upon the conditions under which the secretion takes place. That is to say, the secretion is partly dependent on the cerebral, partly on the sympathetic, nervous system. In consequence of this dependence the two dis- tinct varieties of submaxillary secretion are distinguished as cliorda- and sympathetic saliva. A third kind of saliva, the so-called para- lytic saliva, is secreted after poisoning with curara or after the severing of the glandular nerves. The difference between chordal and sympathetic saliva (in dogs) consists chiefly in their quantitative constitution, namely, the less abundant sympathetic saliva is more viscous and richer in solids, especially in mucin, than the more abundant chordal saliva. The specific gravity of the chordal saliva of the dog is 1.0039-1.0046 and contains from 12 to 14 p. m. solids (ECKHARD). The sympathetic has a specific gravity of 1.0075-1.018, with 16-28 p. m. solids. The gases of the chordal saliva have been investigated by PFLUGER. He found 0.5-0.8$ oxygen, 0.9-1$ nitrogen, and 64.73-85.13$ carbon DIGESTION. 109 dioxide— all results calculated at 0° C. and 760 mm. pressure. The greater part of the carbon dioxide was chemically combined. The two kinds of submaxillary secretion just named have not thus far been separately studied in man. The secretion may be excited by a psychological conception, by mastication, and by irri- tating the mucous membrane of the mouth, especially with acid- tasting substances. The submaxillary saliva in man is ordinarily clear, rather thin, a little ropy, and froths easily. Its reaction is alkaline. The specific gravity is 1.002-1.003, and it contains 3.6-4.5 p. m. solids. We find as organic constituents mucin, traces of albumin and diastatic enzyme is absent in several species of animals. The inorganic bodies are alkali chlorides, sodium and magnesium phosphates, besides bicarbonates of the alkalies and calcium. OEHL finds 0.036 p. m. potassium sulphocyanide in this saliva. The Sublingual Saliva. The secretion of this saliva is also influenced by the cerebral and the sympathetic nervous system. The chord al saliva, which is secreted only to a small extent, contains numerous salivary corpuscles, but is otherwise transparent and very ropy. Its reaction is alkaline and contains, according to HEIDENHAI^, 27.5 p. m. solids (in dogs). The sublingual secretion in man has been investigated by OEHL. It was clear, mucilaginous, more alkaline than the submaxillary saliva, and contained mucin, diastatic enzymes, and potassium sulphocyanide. Buccal mucus can only be obtained pure from animals by the method of BIDDER and SCHMIDT, which consists in tying the exit to all the large salivary glands and cutting off their secretion from the mouth. The quantity of liquid secreted under these circum- stances (in dogs) was so very small that the investigators named were able to collect only 2 grins, buccal mucus in the course .of twenty-four hours. It is a thick, ropy, sticky liquid containing mucin; it is rich in form -elements, above all in flat epithelium- cells, mucous cells, and salivary corpuscles. The quantity of solids in the buccal mucus of the dog is, according to BIDDER and SCHMIDT, 9.98 p. m. Parotid Saliva. The secretion of this saliva is also partly de- pendent on the cerebral nervous system (n. glossopharyngeus) and partly on the sympathetic. The secretion may be excited by 170 PHYSIOLOGICAL CHEMISTRY. mental emotions and by irritation of the glandular nerves, either di- rectly (in animals) or reflexly, by mechanical or chemical irritation of the mucous membrane of the mouth. Among the chemical irri- tants the acids take first place, while alkalies and pungent substances have little action. Sweet-tasting bodies, such as honey, are said to have no effect. Mastication has great influence in the secretion of parotid saliva, which is especially marked in certain herbivora. Human parotid saliva may be collected by the introduction of a canula into the parotid duct. This saliva is thin, less alkaline than the submaxillary saliva (the first drops are sometimes neutral or acid), without special odor or taste. It contains a little albumin but no mucin, which is to be expected from the construction of the gland. It also contains a diastatic enzyme, which, however, is ab- sent in many animals. The quantity of solids varies between 5 and 16 p. m. The specific gravity is 1.003-1.012. Potassium sulpho- cyanide seems to be present, though it is not a constant constituent. KULZ found 1.46$ oxygen, 3.2$ nitrogen, and in all 66.7$ carbon dioxide in human parotid saliva. The quantity of closely-com- bined carbon dioxide was 62$. The mixed buccal saliva in man is a colorless, faintly opales- cent, slightly ropy, easily frothing liquid without special odor or taste. It is made turbid by epithelium cells, mucous and salivary corpuscles, and often by food residues. Like the submaxillary and parotid saliva, on exposure to the air it becomes covered with an incrustation consisting of calcium carbonate and a small quantity of an organic substance, or it gradually becomes cloudy. Its reaction is alkaline, but occasionally also acid. According to STICKER, fresh saliva may be acid a few hours after a meal. Two or three hours after breakfast and four to five hours after dinner the maximum of acidity occurs, and it may also be faintly acid from midnight to morning. The specific gravity varies between 1.002 and 1.009, and the quantity of solids between 5 and 10p.m. The solids, irrespective of the form-constituents mentioned, consist of albumin, mucin., ptyalin, and mineral bodies. It is also claimed that urea is a normal constituent of the saliva. The mineral bodies are alkali chlorides, bicarbonates of the alkalies and calcium, phos- phates, and traces of sulphates and sulphocyanides. DIGESTION. 171 Sulphocyanides, which, although not constant, occur in the saliva of man and certain animals, may be easily detected by first acidifying the saliva with hydrochloric acid and treating with a very dilute solution of ferric chloride. To make the test more conclusive it is best, as control, to take an equal quantity of acidified water and then add ferric chloride. Another, simpler method, proposed by GSCHEIDLEN, consists in putting in a drop or two of the saliva on filter-paper which has previously been dipped in an amber- colored solution of ferric chloride containing hydrochloric acid, and then dried. Each drop of saliva containing sulphocyanide will give a reddish spot. If the quantity of sulphocyanide is so small that it cannot be detected directly, concentrate the saliva after the addition of a little alkali, acidify strongly with hydrochloric acid, and shake repeatedly with ether, evaporating the latter after the addition .of water containing alkali over a gentle heat; then test the remaining liquid. Ptyalin, or salivary diastase, is the amylolytic ferment of the saliva. This ferment is found in human saliva, but not in that of animals. It occurs not only in adults, but also in new-born infants. ZWEIFEL claims that the ptyalin in new-born infants occurs only in the parotid gland, but not in the submaxillary. In the latter it appears only two months after birth. Ptyalin has not been isolated in a pure form up to the present time. It was obtained purest by precipitation with calcium phos- phate (COHNHEIM). For the study or the demonstration of the action of ptyalin we may use a watery or glycerin extract of the salivary glands, or, more simply, the saliva itself. Ptyalin, like other enzymes, is characterized by its action. This consists in converting starch into dextrin and sugar. According to the common acceptation, soluble starch is first formed by this action and then erythro-dextrin, which is further changed so that we obtain at the end achroo-dextrin and maltose with a small admix- ture of glucose. Like starch, glycogen is also split by ptyalin into dextrin and sugar (apparently maltose) upon the addition of water. Ptyalin is not identical with malt diastase; while the first acts with greatest energy at about -f 40° 0., diastase acts best at + 50° to 55° 0. (CHITTENDEN and MARTIN). Ptyalin acts in faintly alkaline, in neutral, and in extremely weak acid solutions. It seems to act most energetically in neutral or, in a few cases, in very faint acid reaction (CHITTENDEN and SCHMIDT). 172 PHYSIOLOGICAL CHEMISTRY. The amount of action in the latter case is dependent upon several circumstances, such as the degree of dilution and the presence of proteids or peptone (CHITTE^DEN). Of the greatest physiological importance, however, is the fact that even very small quantities of free acids — not only a degree of acidity of about 1 p. m. HC1, which often occurs in the gastric juice, but even a smaller quantity of hydrochloric acid (organic acids are less active) — at once pre- vents the action of the ptyalin and destroys the enzyme. It is also of interest that boiled starch (starch-paste) is more quickly con- verted into sugar than unboiled. The time required to change unboiled starch varies with the kind. The rapidity with which ptyalin acts increases, at least tinder conditions otherwise favorable, with the amount of enzyme and a temperature a little above -\- 40° C. Foreign substances such as metallic salts, have a different effect. Certain salts even in small quantities completely arrest the action; for example, HgCl2 accom- plishes this result by the presence of only 0.05 p. m. Other salts, such as magnesium sulphate, in small quantities (0.25 p. m.) acceler- ate, and in larger quantities (5 p. m.) check the action. The accu- mulation of the products of the amylolytic decomposition also checks the action of the saliva. To show the action of saliva or ptyalin on starch the three ordinary tests for glucose may be used, namely, MOORE'S or TROMMER'S test or the Bismuth test (see Chap. XIV). It is also necessary, as a control, to first test the starch-paste and the saliva for the presence of glucose. The quantitative composition of the mixed saliva must vary con- siderably, not only because of individual differences, but also because under varying conditions there may be an unequal division of the secretion from the different glands. Analyses of the composition of human saliva are given in the table on the opposite page. The figures are parts per 1000. The quantity of saliva secreted during 24 hours cannot be exact- ly determined, but has been calculated by BIDDER and SCHMIDT to be 1500 grms. The most abundant secretion occurs during meal-times. According to the calculations and determinations of TUCZEK in man, 1 grm. of gland should yield 13 grms. secretion in the course of one hour during mastication. These figures DIGESTION. 173 03 B M H JACUBOWITSCH FRERICHS. TIEDEHANN and GMELIN. HERTER. LKHMANM. ii Water 992.9 995 10 994 1 988 3 994 7 004 2 Solids 7.1 4.84 59 11 7 5 3 35-84 5 ft JVEucus and epithelium. . . . 1 4 1 62 2 13 in filtered saliva 0 0 Soluble organic substances.. (Ptyalin of early investigators) Sulphocyanides ..... 3.8 1.34 0 06 1.42 0 10 3.27 0 064 1.4 0 04 Salts 1 9 1 82 2 19 1 OS to 0.09 2 2 HAMMERBACHER found in 1000 parts of the ash from human saliva (potash 457.2, soda 95 9, iron oxide 50.11, magnesia 1.55, sulphuric anhydride SO3) 63.8, phosphoric anhydride (PaOB) 188.48, and chlorine 183.52. correspond fairly well with those representing the average secre- tion from 1 grin, of gland in animals, namely, 14.2 grms. in the horse and 8 grms. in oxen. The quantity of secretion per hour may be 8 to 14 times greater than the entire mass of glands, and there is probably no gland in the entire body, as far as we know at present — the kidneys not excepted — whose ability of secretion under physiological conditions equals that of the salivary glands. A re- markably abundant secretion of saliva is induced by pilocarpin, while atropin, on the contrary, prevents it. Though an abundant secretion of saliva is produced, as a rule, by an increased supply of blood, still it is not a simple nitration process, as seen from the following circumstances. The secretion- pressure is greater than the blood -pressure m the carotid, and in poisoning by atropin, which paralyzes the secretory nerves, an in- creased supply of blood is produced by irritation of the chorda, but no secretion (HEIDENHAIN). The salivary glands have moreover a specific property of eliminating certain substances, such as potas- sium salts (SALKOWSKI), iodine and bromine combinations, but not others, such as iron combinations. It is also noticeable that the saliva is richer in solids when it is eliminated quickly by gradually- increased irritation, and in larger quantities than when the secre 174 PHYSIOLOGICAL CHEMISTRY. tion is slower and less abundant (HEIDENHAIN). The amount of salts increases also to a certain degree by an increasing rapidity of elimination (HEIDENHAIN, WERTHER, LANGLEY and FLETCHER, NOVY). The chemical changes taking place during secretion are un- known, but it is probable that, like the secretion processes in gen- eral, the secretion of saliva is closely connected with the processes in the cells. HEIDEKHAIN claims that the mucin-cells of the sub- maxillary gland are destroyed (while EWALD and STOHR claim that they only lose mucin), and in the period of rest the mucin reap- pears in these cells. These observations still do not throw any light upon the chemical processes going on. The Physiological Importance of the Saliva. The quantity of water in the saliva renders possible the effects of certain bodies on the organs of taste, and it also serves as a solvent for a part of the nutritive substances. The importance of the saliva in mastication is especially marked in herbivora, and there is no question of its importance in facilitating the act of swallowing. The power of converting starch into sugar does not belong to the saliva of all animals, and even when it possesses this property the intensity varies in different animals. In man, whose saliva forms sugar ra- pidly, a formation of sugar from (boiled) starch undoubtedly takes place in the mouth, but how far this action goes on after the morsel has entered the stomach depends upon the rapidity with which the acid gastric juice mixes with the swallowed food, and also upon the relative amounts of the gastric juice and food in the stomach. The large quantity of water which is swallowed with the saliva must be absorbed and pass into the blood, and it must go through an intermediate circulation in the organism. Thus the organism pos- sesses in the saliva an active medium by which a constant stream, conveying the dissolved and finely-divided bodies, passes into the blood from the intestinal canal during digestion. Salivari Calculi. The so-called tartar is yellow, gray, yellowish gray, brown or black, and has a stratified structure. It may contain more than 200 p. m. organic substances, which consist of mucin, epithelium, and LEPTO- THKIX. The chief part of the inorganic constituents consists of calcium car- bonate and phosphate. The salivary stone may vary in size from the size of a small grain to that of a pea or still larger (a salivary stone has been found weighing 18.6 grms.), and it contains a variable quantity of organic substances, 50-380 p. m., which remain on extracting the stone with hydrochloric acid. The chief inorganic constituent is calcium phosphate. DIGESTION. 175 II. The Glands of the Mucous Membrane of the Stomach, and the Gastric Juice. Since of old, the glands of the mucous coat of the stomach have been divided into two distinct kinds. The kind occurring in the greatest abundance, and the most important in size, especially in the fundus, has been named fundus glands, also RENNET or PEPTONE glands. The other, which is found in the neigh- borhood of the pylorus, has received the name of pyloric glands, sometimes also, though incorrectly, mucous glands. The mucous coating of the stomach is covered to its entire extent with a layer of cylindrical epithelium, which is considered as consisting exclu- sively of follicles, small cup-shaped cavities which by a mucus-like metamorphosis produce the protoplasm. The fundus glands contain two kinds of cells: ADELOMOR- PHIC or principal cells, and DELOMORPHIC or PARIETAL cells, the latter formerly called RENNET or pepsin cells. Both kinds consist of protoplasm rich in proteids; but their relationship to coloring matters seems to show that the two albuminous bodies are not identical. The nucleus must consist chiefly of nuclein. Besides the above-mentioned constituents the fundus glands con- tain as more specific constituents two zymogens, which are the mother-substances of the pepsin and the rennet, besides a small quantity of fat and cholesterin. The pyloric glands contain cells which are generally considered as related to the above-mentioned chief cells of the fundus glands. As these glands were formerly thought to contain a larger quantity of mucin, they were also called mucous glands. According to HEIDENHAIN, independent of the cylindrical epithelium of the excretory passages, they take no part worthy of mention in the formation of mucus, which, according to his views, is effected by the epithelium covering the mucous membrane. The pyloric glands also seem to contain the zymogens referred to above. Alkali chlorides, alkali phosphates, and calcium phosphates are found in the mucous coating of the stomach. The Gastric Juice. The experiments of HELM and BEAUMONT on persons with gastric fistula led to the suggestion that gastric fistulas be made on animals, and this operation was first performed 176 PHYSIOLOGICAL CHEMISTRY. by BASSOW in 1842 on a dog. VERNEUIL performed the same on a man in 1877 with successful results. These fistulas in animals afford an excellent means of studying the secretion of gastric juice and also the stomachic digestion. In a fasting condition the mucous coat is often nearly dry; sometimes, especially on certain herbivora, it is covered with a layer of viscid so-called mucus. If food is introduced into the stomach, or if the mucous membrane is irritated in some way, then a secre- tion of a thin, acid fluid, the real gastric juice, takes place. The secretion may be produced by mechanical or thermal irritation (in- troduction of cold water or pieces of ice into the stomach), or by chemical irritants. Among the latter, alcohol and ether when in too great concentration do not produce a physiological secretion, but a transudation of a neutral or faintly alkaline fluid con- taining albumin (CL. BERNARD). To this class of irritants belong carbon dioxide and hydrochloric acid; the last especially increases the secretion of pepsin (JAWORSKY), spices, meat extracts, neutral salts, such as NaCl (which acts like alcohol in too great concentration), and alkali carbonates. The alkali carbonates are supposed by certain investigators to first neutralize, and then produce a continuous secretion of, acid gastric juice. The statements in regard to the action of different bodies in the secretion of gastric juice are still rather uncertain and often contradictory. Several investigators state that the secretion of gastric juice may be stimulated by reflex means. After the introduction of water into the stomach a proportionally poor and scanty secretion takes place, while on the contrary the introduction of digestible foods causes a more abundant and continuous secretion (SCHIFF, HEIDENHAIN). But in these cases the secretion does not take place immediately, but only after the absorption of the soluble bodies has commenced. This fact justifies the usual custom of commencing a meal with fluid nutritives such, as soup. The Qualitative and Quantitative Composition of the Gastric Juice. The gastric juice, which can hardly be obtained pure and free from residues of the food or from mucus and saliva, is a clear, or only very faintly cloudy, and in man nearly colorless fluid of an insipid, acid taste and strong acid reaction. It contains, as form- DIGESTION. 177 elements, glandular cells or their nuclei, mucus-corpuscles, and more or less changed cylindrical epithelium. The acid reaction of the gastric juice depends on the presence of free acid, which, as we have learned from the investigations of C. SCHMIDT, EICHET, and others, seems, under physiological con- ditions, to consist only of hydrochloric acid. Under special con- ditions, as after a meal rich in carbohydrates, lactic acid occurs in the contents of the stomach, and even acetic and butyric acid. The quantity of free hydrochloric acid in the gastric juice of sheep is about 1.2 p. m., and in dogs 3 p. m. KICHET found as average for 80 determinations of human gastric juice 1.7 p. m. free hydrochloric acid, with a variation between 0.5 and 3 p. m. According to SZABO and EWALD and BOAS, the human gastric juice contains usually about 2-3 p. m. HC1. The gastric juice seems to contain no coagulable albumin, but contains traces of peptone or albumose (?). Among the organic bodies a little mucin is found, and besides, especially in man, two enzymes, pepsin and rennet. The specific gravity of gastric juice is low, 1.001-1.010. It is therefore correspondingly poor in solids. As examples of the composition of different kinds of gastric juice the analyses of C. SCHMIDT are here given. It must be remarked that the human gastric juice analyzed was diluted by saliva and water and should therefore not be considered as normal. The figures are parts per 1000. Human Gas- tric Juice mixed with Saliva. Gastric Juice from Dog free from Saliva. Gastric Juice from Dog contain- ing Saliva. Gastric Juice of Sheep. Water 994.40 973.0 971.2 986.15 Solids 5.60 27.0 28.8 13.85 3.19 17.1 17.3 4.05 NaCl 1.46 2.5 3.1 4.36 CaCla 0.06 0.6 1.7 0.11 KC1 0.55 1.1 1.1 1.52 NH4C1 0.5 0.5 0.47 Free hydrochloric acid (HC1). . . Cas(PO4)2 0.20 ) 3.1 1.7 2.3 2.8 1.23 1.18 MO-JPO^O )• 0.12 0.2 0.3 0.57 FePO4 ) 0.1 0.1 0.33 178 PHYSIOLOGICAL CHEMISTRY. The physiologically important constituents, besides free Hydro- chloric acid, are pepsin and rennet. Pepsin. This enzyme is found, with the exception of certain fishes, in all vertebrata thus far investigated. Pepsin occurs in adults and in new-born infants. This condi- tion is different in new-born animals. While in a few herbivora, such as the rabbit, pepsin occurs in the mucous coat before birth, this enzyme is entirely absent at the birth of those carnivora which have thus far been examined, such as the dog and cat. In various invertebrates a fermeut has also been found which has a prote- olytic action in acid solutions. KRUKENBERG has shown that this enzyme, nevertheless is not in all animals, identical with ordinary pepsin. DARWIN has further found that certain plants which feed upon insects secrete an acid juice which dissolves albumin, but it is still doubtful whether these plants contain any pepsin, v. GORUP BESANEZ has isolated from vetch-seed an enzyme which acts like pepsin, but whose identity with pepsin is doubtful. Pepsin is as difficult to isolate in a pure condition as other enzymes. The purest pepsin was that prepared by BRUCKE and SUNDBERG ; this gave negative results with most reagents for albumin. Pepsin, therefore, does not seem to be a true albuminous substance. It is, at least in the impure condition, soluble in water and glycerin. It is precipitated by alcohol, but only slowly de- stroyed. It is quickly destroyed by heating its watery solution to boiling ; in the dry state it can, on the contrary, be heated to over 100° C. without losing its physiological action. The only property which is characteristic of pepsin is that it dissolves albuminous bodies in acid, but not in neutral or alkaline, solutions with the formation of albumoses and peptones. The methods for the preparation of relatively pure pepsin de- pend, as a rule, upon its property of being thrown down with finely-divided precipitates of other bodies, such as calcium phos- phate or cholesterin. The rather complicated method of BRUCKE and SUNDBERG is based upon this property. A relatively pure pepsin solution intended for digestion tests and of effective action may be prepared by the following method as suggested by MALY. The mucous membrane (of the pig's stomach) is treated with water containing phosphoric acid, and the filtrate precipitated by lime- water; the precipitate, which contains the pepsin, is then dissolved in water by the addition of hydrochloric acid, and the salts removed by dialysis, by which means the pepsin which does not diffuse re- mains in the dialyzer. A pepsin solution somewhat impure but DIGESTION. 179 active and not liable to spoil may be obtained if, as suggested by v. WITTICHS, we extract the finely-divided mucous membrane with glycerin, or better with glycerin which contains 1 p. m. HC1. To each part by weight of the mucous coat add 10-20 parts glycerin. This is filtered after 8-14 days. The pepsin (together with mucli albumin) may be precipitated by alcohol from this extract. If this extract is to be used directly for digestion tests, then to 100 c. c. of water which has been acidified with 1-4 p. m. HC1 add 2-3 c. c. of the extract. For digestion tests an infusion of the mucous membrane of the stomach may be used directly in many cases. The mucous coat is carefully washed with water (if a pig's stomach is used) and finely cut; if a calfs stomach is employed, only the upper layer of the mucous coat is scraped off with a watch-glass or the back of a knife. The pieces of mucous membrane or the slimy masses obtained by scraping are rubbed with pure quartz-sand, treated with acidified water, and allowed to stand for 24 hours in a cool place and then filtered. In the preparation of artificial gastric juice that part only of the mucous coat richest in pepsin is used, the pyloric is of little value. A strong, impure infusion may generally be obtained from the pig's stomach, while a relatively pure and powerful infusion is ob- tained from the stomach of birds (hens). The stomachs of fish (pike) also yield a tolerably pure and active infusion. An active and rather pure artificial gastric juice may be prepared by scraping the inner layers of a calfs stomach from which the pyloric end has been removed. For a medium-sized calfs stomach 1000 c. c. of acidified water must be used. The degree of acidity required in the infusion depends upon the use to which the gastric juice is to be put. If it is to be em- ployed in the digestion of fibrin, an acidity of 1 p. m. HC1 must be selected, while, on the contrary, if it is to be used for the diges- tion of hard -boiled -egg albumin, an acidity of 2-3 p. m. HC1 is preferable. This last-mentioned degree of acidity is generally the better, because the infusion is preserved thereby, and at all events it is so rich in pepsin that it may be diluted with water until it has an acidity of 1 p. m. HC1 without losing any of its solvent action on unboiled fibrin. The Action of Pepsin on Proteids. Pepsin is inactive in neutral or alkaline reactions, but in acid liquids it dissolves coagulated albuminous bodies. The proteid always swells and becomes trans- parent before it dissolves. Unboiled fibrin swells up in a solution containing 1 p. m. HC1, forming a gelatinous mass, and does not dissolve at ordinary temperature within a couple of days. Upon 180 PHYSIOLOGICAL CHEMISTRY. the addition of a little pepsin, however, this swollen mass dissolves, quickly at an ordinary temperature. Hard-boiled-egg albumin, cut in thin pieces with sharp edges, is not perceptibly changed by dilute acid (2-4 p. m. HC1) at the temperature of the body in the course of several hours. But the simultaneous presence of pepsin causes the edges to become clear and transparent, blunt and swollen, and the albumin gradually dissolves. From what has been said above in regard to pepsin, it follows that albumin may be employed as a means of detecting pepsin in liquids. ' Fibrin may be employed as well as hard-boiled-egg albu- min, which latter is used in the form of slices with sharp edges. As the fibrin is easily digested at the normal temperature, while the pepsin test with egg-albumin requires the temperature of the body, and as the test with fibrin is somewhat more delicate, it is often preferred to that with egg-albumin. When we speak of the "pepsin test" without further explanation, we ordinarily under- stand it as the test with fibrin. This test nevertheless requires care. The unboiled fibrin may be dissolved by acid alone without pepsin, but this generally requires more time. In testing with unboiled fibrin at normal temperature, it is advisable to make a control test with another portion of the same fibrin with acid alone. Since at the tempera- ture of the body unboiled fibrin is easier dissolved by acid alone, it is best always to work with boiled fibrin. As pepsin has not, thus far, been prepared in a positively pure condition, it is impossible to determine the absolute quantity of pepsin in a liquid. It is only possible to compare the relative amounts of pepsin in two or more liquids, which may be done in several ways. As the best of these we give the following method as suggested by BRUCKE. If two pepsin solutions A and B are to be compared with each other relatively to the amounts of pepsin they contain, they must first be brought to the proper degree of acidity, about 1 p. m. HC1, care being taken that one is not more diluted than the other. Then prepare a large number of specimens of each solution by diluting with hydrochloric acid of 1 p. m. HC1, so that they contain respectively ^, £, £, ^, •£$, and so on, the amount of pepsin in the original liquid being 1. If the original quantity of pepsin in the two liquids is designated by p and p', we then have the two series of liquids : A lp DIGESTION. 187 Then a small piece of boiled-egg albumin, obtained by cutting thin slices with a cork-cutter, is placed in each test, or a small flake of fibrin is added. Of course care must be taken to add the same-sized slice of egg-albumin or flake of fibrin. Now observe and note exactly the time when each test of the two series begins to digest and when it ends, and it will be found that certain tests of one series make about the same progress as certain tests of the other series. It may be inferred from this that they contain about the same quantity of pepsin. As example, it is found in one series of tests that the digestive rapidity of the tests p £, p ^ , p -fa is about the same as the tests p' 4, p |, p' \\ therefore we conclude that the liquid A is about four times as rich in pepsin as the liquid B. The rapidity of the pepsin digestion depends on several circum- stances. Thus different acids are unequal in their action; hydro- chloric acid shows a more powerful action than any other, whether an organic or an inorganic acid. The degree of acidity is also of the greatest importance. The most effective degree of acidity is not the same with hydrochloric acid for different albuminous bodies. For fibrin it is 0.8-1 p. m., for myosin, casein, and vege- table albumin about 1 p. m., for hard-boiled-egg albumin, on the contrary, about 2.5 p. m. The rapidity of the digestion increases, at least to a certain point, with the quantity of pepsin present, un- less the pepsin added is contaminated by a large quantity of pro- ducts of digestion, which may prevent its action. The accumulation of products of digestion disturb the digestion. At low temperatures the pepsin acts slowly in warm-blooded animals, and it is nearly without action at a temperature below -f 3° C. With increasing temperature the rapidity of digestion increases, and at about 40° C. it is greatest. The pepsin of cold-blooded animals also acts in the neighborhood of 0° C. If the swelling up of the proteid is pre- vented, as by the addition of neutral salts, such as NaCl in sufficient amounts, or by the addition of bile to the acid liquid, the digestion can be prevented. Foreign bodies of different kinds can produce different actions, in which naturally the variable quantities in which they are added are of the greatest importance. Salicylic acid and carbolic acid hinder the digestion, while arsenious acid promotes it (CHITTENDEN), and hydrocyanic acid is relatively indifferent. Alcohol in large quantities (10$ and above) disturbs the digestion, while small quantities act indifferently. Metallic salts in very small quantities may indeed sometimes accelerate digestion, but otherwise they tend to retard it. The action of metallic salts in different cases can be explained in different ways, but they often seem to form 182 PHYSIOLOGICAL CHEMISTRY. with proteids insoluble or difficultly-soluble combinations. The alkaloid compounds may also decrease the pepsin digestion (CHIT- TENDED and ALLEN). A very large number of observations have been made in regard to the action of foreign substances on artificial pepsin digestion, but as these observations have not given any direct result in regard to the action of these same substances on natural digestion, we will not here further discuss them. The Products of the Digestion of Proteids by Means of Pepsin and Acid. In the digestion of nucleo-albumin an insoluble residue of nuclein always remains. Fibrin also yields an insoluble residue, which consists, at least in great part, of nuclein, derived from the form-elements enclosed in the blood-clot. This residue which remains in the digestion of certain albuminous bodies is called dyspeptone by MEISSNER. If the solution is filtered after a com- plete digestion and neutralized, it gives in different cases a more or less abundant precipitate of acid albuminate, or a mixture of albuminates called parapeptone by MEISSKER. After filtering this precipitate and concentrating the filtrate again, some proteid often separates. If this precipitate be filtered, the filtrate will now con- tain albumoses and peptones in the ordinary sense, while the so- called true peptone of KUHNE may sometimes be entirely absent, and in general is obtained in quantity worth mentioning only after a more continuous and intensive digestion. The relationship between the albumoses and peptones in the ordinary sense changes very much in different cases and in the digestion of various albuminous bodies. For instance, a larger quantity of primary albumoses is obtained from fibrin than from hard-boiled-egg albu- min or from the proteids of meat. In the digestion of unboiled fibrin an intermediate product may be obtained in the earlier stages of the digestion — a globulin which coagulates at -j- 55° C. (HASE- BROEK). For information in regard to the different albumoses and peptones which are formed in pepsin digestion, the reader is referred to previous pages (25-28.). KUHNE claims that the albumoses and peptones are the final products in the pepsin digestion. HOPPE-SEYLER claims, on the contrary, that amido-acids, leucin and tyrosin, are also found. HIRSCHLER has tried to confirm this view by his investigations. The methods used by him do not seem to be quite trustworthy DIGESTION. 133 (NEUMEISTER), and according to KUHNE and NEUMEISTER the amido-acids found in the specified cases as products of digestion were derived from the contamination of the gastric juice employed. Action of Pepsin Hydrochloric Acid on other Bodies. The gela- tine-forming substance of the connective tissue, of the cartilage and of the bones, from which last the acid only dissolves the inorganic substances, is converted into gelatine by digesting with gastric juice, The gelatine is further changed so that it loses its property of gelatinizing and is converted into a so-called gelatine peptone (see page 38). True mucin (from the submaxillary) is dissolved by the gastric juice and yields a substance similar to peptone and a reducible substance similar to that obtained by boiling with a mineral acid. Elastin is dissolved more slowly and yields the above-mentioned substance (page 36). Keratin and the epidermis formation are insoluble. Nuclein is not dissolved and the cell- nucleus is therefore insoluble in gastric juice. The animal cell- membrane is, as a rule, more easily dissolved the nearer it stands to elastin, and it dissolves with greater difficulty the more closely it is related to keratin. The membrane of the plant-cell is not dissolved. The oxyhcemoglobin is changed into haematin and acid albuminate, the latter undergoing further digestion. It is for this reason that blood is changed into a dark-brown mass in. the stomach. The gastric juice does not act on fat, but, on the contrary, on fatty tissue in which it dissolves the cell-membrane, setting the fat free. Human gastric juice, according to LEUBE, can convert cane-sugar into grape-sugar. Pepsin hydrochloric acid does not seem to take any part in the decompositions and cleavages, which the carbo- hydrates may undergo in the stomach. Pepsin alone, as above stated, has no action on proteids, and an acid of the intensity of the gastric juice can only very slowly, if at all, dissolve coagulated albumin at the temperature of the body. Pepsin and hydrochloric acid together not only act more quickly, but qualitatively they act otherwise than the acid alone. If liquid proteid is digested with hydrochloric acid of 2 p. m., it is con- verted into acid albuminates; but if the acid is replaced by pepsin, the formation of syntonin takes place essentially slower under the same conditions (MEISSNER). From this it is inferred that a part of the hydrochloric acid is combined with the pepsin, and we have 184 PHYSIOLOGICAL CHEMISTRY. here a proof of the existence of a paired acid, called by C. SCHMIDT pepsin hydrochloric acid. It has been further suggested that this hypothetical acid is possibly decom- posed into pepsin and a free acid, which in statu nascendi dissolves proteids to a certain degree. The pepsin set free reunites with a new portion of acid, forming pepsin hydrochloric acid, and in contact with proteids is further decomposed as above described. It is hardly necessary to mention that this statement is only an unproved hypothesis. Rennet or CHYMOSIN (DESCHAMPS) is the second enzyme of the gastric juice. According to BOAS, it is found in human gastric under physiological conditions, but may be absent under special pathological conditions, such as carcinoma, atrophy of the mucous membrane, and certain chronic catarrhs (BOAS, JOHNSON, KLEM- PEKER). It is habitually found in the neutral, watery infusion of the fourth stomach of the calf and sheep, especially in an infusion of the fundus part. In other mammalia and in birds it is seldom found, and in fishes hardly ever in the neutral infusion. Instead of this rennet-forming substance a rennet zymogen, from which the rennet is formed by the action of an acid is always found. Kennet is just as difficult to prepare in a pure state as the other enzymes. The purest rennet enzyme thus far obtained did not give the ordinary albumin reactions. On heating its solutions it is de- stroyed, and indeed more easily in acid than in neutral solutions. If an active and strong infusion of a mucous coat in water contain- ing 3 p. m. HC1 is heated to 37-40° C. for 48 hours, the rennet is destroyed, while the pepsin remains. A pepsin solution free from rennet can be obtained in this way. Rennet is characterized by its physiological action, which consists in coagulating milk or a casein solution containing lime, if neutral or faintly alkaline. Kennet may be carried down by other precipitates like other enzymes, and thus may be obtained relatively pure. It may also be obtained, contaminated with a great deal of proteids, by extracting the mucous coat of the stomach with glycerin. A comparatively pure solution of rennet may be obtained in the following way. An infusion of the mucous coat of the stomach in hydrocholoric acid is prepared and then neutralized, after which it is repeatedly shaken with new quantities of magnesium carbonate until the pepsin is precipitated. The filtrate, which should act strongly on milk, is precipitated by basic lead acetate, the preci- pitate decomposed with very dilute sulphuric acid, the acid liquid filtered and treated with a solution of stearin soap. The rennet is DIGESTION. 186 thrown down by the fatty acids set free, and when these last are placed in water and removed by shaking with ether, the rennet re* mains in the watery solution. A fasting animal cannot secrete a strongly-acid gastric juice The acid of the gastric juice then cannot be derived from the foods, but must originate in the mucous coat. As the pyloric glands, which contain no delomorphic cells, secrete an alkaline secretion, while the fundus glands, in which such cells occur, yield an acid secretion, it is generally assumed with HEIDENHAIN that the delo- morphic cells are of special importance in the secretion of free hydrochloric acid — a statement which other observations tend to confirm. That the hydrochloric acid must originate from the chlo- rides of the blood is evident; and these latter must therefore un- dergo a decomposition with the setting free of hydrochloric acid. This decomposition has been considered as an electrolysis, but the opinion has also been held that it may be caused by some organic acid formed in the mucous membrane (BRUCKB). MALT has called attention to the fact that, on account of the presence of a large quantity of free carbon dioxide in the blood and the avidity of the same, there must be present among the numerous combinations of acids and bases which exist in the serum traces of free hydrochloric acid in addition to acid salts. As these traces of hydrochloric acid are separated from the blood by means of rapid diffusion by the glands, the action of the carbon dioxide must set free new traces of hydrochloric acid in the blood, and in this way may be explained the secretion in the blood of large quantities of hydrochloric acid. But though the occurrence of traces of free hy- drochloric acid in the alkaline blood is not denied, it does not fol. low that the hydrochloric acid passes by diffusion from the blood to the gastric juice. Similar processes in other animal glands render it probable that here, as in other cases of secretion, we have to deal with a yet unexplained specific secretory action of the glandular cells. The process going on in the secretion of hydro- chloric acid is not yet understood. After an abundant meal, when the store of pepsin in the stom- ach is completely exhausted, SCHIFF claims that certain bodies, especially dextrin, have the property of causing a supply of pepsin in the mucous membrane. This " charge theory/' though experi- 186 PHYSIOLOGICAL CHEMISTRY. mentally proved by several investigators, has nevertheless not yet been confirmed. On the contrary, the statement of SCHIFF that a substance forming pepsin, a " pepsinogen" or "propepsin" occurs in the ventricle has been proved. LANGLEY has shown positively the existence of such a substance in the mucous coat. This substance, propepsin, shows a comparatively strong resistance to dilute alkalies (a soda solution of 5 p. m.) which easily destroys pepsin (LANGLEY). Pepsin, on the other hand, withstands better than propepsin the action of carbon dioxide, which quickly destroys the latter (LANG- LEY). The occurrence of a rennet zymogen in the mucous coat has been mentioned above, The question in which cells the two zymogens, especially the propepsin, are produced has been extensively discussed for several years. Formerly it was the general opinion that the delomorphic cells were pepsin-cells, but at the present time the theory univer- sally prevails, based chiefly on the experiments of HEIDENHAIN" and his pupils, supported by LANGLEY and others, that the forma- tion of pepsin goes on in the adelomorphic or principal cells. The Pyloric Secretion. That part of the pyloric end of the dog's stomach which contains no fundus glands was dissected by KLEMENSIEWICZ, one end being sewed together in the shape of a blind sack and the other sewed into the stomach. From the fistula thus created he was able to obtain the pyloric secretion of a living animal. This secretion is alkaline, viscous, jelly-like, rich in mucin, of a specific gravity of 1.009-1.010, and containing 16.5-20.5 p. m. solids. It has no effect on fat, but acts, though very slowly, on starch, converting it into sugar, and habitually contains pepsin in rather large amounts. HEIDENHAIN has observed the same in permanent pyloric fistula. The secretion of the juices of the stomach is dependent to a great extent upon the excitement acting on the mucous coat of the stomach, and it follows from this that the quantity of secretion under different conditions must vary considerably. The statements of the quantity of gastric juice secreted in a certain time are there- fore so unreliable that they need not be taken into account. The Chyme and the Digestion in the Stomach. By the move- ments of the walls of the stomach the contents are kneaded and the food-particles pressed against each other and divided. By means of DIGESTION. 187 this mechanical irritation of the mucous coat of the stomach, as well as by the chemical irritation caused by the food and saliva, an increased secretion of gastric juice occurs. The food is thereby freely mixed with liquid and is gradually converted into a pulpy mass, the chyme. This mass is acid in reaction, and with the ex- ception of the interior of large pieces of meat or other solid foods, the chyme is acid throughout. The metabolism products in the digestion of proteids and carbohydrates may always be detected in the chyme ; likewise more or less changed undigested residues of swallowed food, which indeed form the chief mass of the consti- tuents of the chyme. In the chyme morsels of FLESH more or less changed are found which, when unboiled flesh is partaken of, may be much swollen and slippery. MUSCLE and CARTILAGE are also often swollen and slippery, while pieces of BONE sometimes show a rough and uneven surface after the digestion has continued for some time, which de- pends upon the fact that the gelatinous substances of the bone are attacked more quickly by the gastric juice than the earthy parts. MILK coagulates in the stomach by the combined action of the rennet enzyme and the acid, but in certain cases by the action of the acid alone. From the relative quantities of the swallowed milk to the other food either large and solid lumps of cheese are formed or smaller lumps or grains which are divided in the pulpy mass. Cow's milk regularly yields large, solid masses or lumps; human milk gives, on the contrary, a fine, loose coagulum or a fine precipitate which is immediately dissolved in part by the acid liquid. The milk-sugar may pass into lactic-acid fermentation, and this accord- ing to RICHET, is the reason why the acid reaction of the contents of the stomach is greater at the end of the digestion of a meal consist- ing mainly of milk. BREAD, especially when not too fresh, is converted rather easily into a pulpy mass in the stomach. Other vegetable foods, such as POTATOES, may, if not sufficiently masticated, often be found in the contents of the stomach, very little changed, several hours after a meal. STARCH is not converted into sugar by the gastric juice, but in the first phases of the digestion, before a large quantity of hydro- chloric acid has accumulated, it seems that the action of the saliva 188 PHYSIOLOGICAL CHEMISTRY. continues, and therefore the presence of dextrin and sugar can bt detected in the contents of the stomach. Besides this the car- bohydrates in the stomach may in part undergo a lactic-acid fer- mentation, probably caused by the micro-organisms present. According to the investigations of ELLENBERGER and HOFF- MEISTER on horses and pigs, after a meal rich in amylaceous bodies in the first phase of the digestion, an AMYLOLTSE takes place with the formation of lactic acid; then gastric juice containing hydro- chloric acid is secreted, then follows a second phase in which the proteolyse takes place. As a rule, the formation of lactic acid de- creases as the secretion of hydrochloric acid increases. EWALD and BOAS claim that a similar condition also exists in human beings. They claim that there is in the first stage of digestion a predominance of lactic acid in the stomach, in the second a simul- taneous occurrence of lactic and hydrochloric acids, and in the third stage almost exclusively hydrochloric acids. KJAERGAARD has lately formed the same conclusions from his investigations on children and robust persons. In persons with altered blood-vessels due to senility the contents of the stomach show chiefly the presence of lactic acid. Such persons digest large amounts of carbohydrates, while the digestion of albuminous bodies is decreased. The FATS which are not fluid at the ordinary temperature melt in the stomach at the temperature of the body and become fluid. In the same way the fat of the fatty structure is set free in the stomach by the gastric juice which digests the cell-membrane. The gastric juice itself seems to have no action on fats, but, according to recent statements, a splitting of the neutral fats into fatty acids and glycerin takes place, though not to a great extent. This splitting is not dependent on the bacteria of the contents of the stomach, or at least only to a small extent (KLEMPERER and SCHEURLEN"). The soluble salts of the food naturally are found dissolved in the liquids of the contents of the stomach ; but the insoluble salts may also be dissolved by the acid of the gastric juice. Since the hydrochloric acid of the gastric juice prevents the contents of the stomach from fermenting with the generation of gas, those gases which occur in the stomach probably depend, at least in great measure, upon the swallowed air and saliva, and upon those gases generated in the intestines and returned through DIGESTION. 180 the pyloric valve. PLANER found in the stomach-gases of a dog 66-68$ N, 25-33$ C08, and only a small quantity, 0.8-6.1$, of oxygen. According as the food is finely or coarsely divided it passes sooner or later through the pylorus into the intestines. From BUSCH'S observations on a human intestinal fistula, it required generally 15-30 minutes after eating for undigested food, such as pieces of meat, to pass into the upper part of the intestines. Among the cases of duodenal fistula in human beings observed by KUHNE, one is mentioned in which he saw, ten minutes after eat- ing, uncurdled but still coagulable milk and small pieces of meat pass out of the fistula. The time in which the stomach unburdens itself of its contents depends, however, upon the rapidity with which the quantity of hydrochloric acid increases, for it seems to act as a sort of irritant and causes the opening of the pylorus (EwALD and others). Many other conditions also come into play, namely, the activity of the gastric juice, the quantity and character of the food, etc., etc., and therefore the time required to empty the stomach must be variable. BICHET observed in a case of stomach fistula that in man the quantity of food which is in the stomach the first three hours is not essentially changed, but that in the course of a quarter of an hour nearly all is driven out, so that only a small residue remains. KUHNE has made about the same obser- vations on dogs and human beings. He found, indeed, in dogs that in the first hour small quantities of meat passed into the intestines every ten minutes ; but he also observed that in dogs, on an ave- rage, about five hours after eating, in man somewhat earlier, a free emptying into the intestines takes place. According to other investigators (EwALD and BOAS), the emptying of the human stomach does not take place suddenly, but gradually. BEAUMONT found in his extensive observations on the Canadian hunter, ST. MARTIN, that the stomach, as a rule, is emptied li-5£ hours after a meal, depending upon the character of the food. The time in which different foods leave the stomach depends upon their digestibility. In regard to the unequal digestibility in the stomach of foods rich in proteids, which really form the object of the action of the gastric juice, a distinction must be made be- tween the rapidity with which the proteids are converted into 190 PHYSIOLOGICAL CHEMISTRY. albumoses and peptones and the rapidity with which the food is converted into chyme, or at least so prepared that it may easily pass into the intestines. This distinction is especially important from a practical standpoint. When a proper food is to be decided upon in cases of diminished stomachic digestion, it is important to select such foods as, independent of the difficulty or ease with which their proteid is peptonized, leave the stomach easily and quickly, and which require as little action as possible on the part of this organ. From this point of view those foods, as a rule, are most digestible which are fluid from the start or may be easily liquefied in the stomach; but these foods are not always the most digestible in the sense that their proteid is most easily peptonized. As an example, hard-boiled white of egg is more easily peptonized than fluid white of egg at a degree of acidity of 1-2 p. m. HC1 ; nevertheless we consider, and justly, that an unboiled or soft-boiled egg is easier to digest than a hard-boiled one. Likewise uncooked meat, when it is not chopped very fine, is not more quickly but more slowly peptonized by the gastric juice than the cooked, but if it is divided sufficiently fine it is often more quickly peptonized than the cooked. The greater or less facility with which the different albuminous foods are peptonized by the gastric juice has been comparatively little studied, and as the conditions in the stomach are more com- plicated, results obtained with artificial gastric juice are often of no value for the practising physician and should in any case be used only with the greatest caution. Under these circumstances we cannot enter more deeply into this subject, but the reader is referred to text-books on dietetics and the theory of foods. As our knowledge of the digestibility of the different foods in the stomach is slight and dubious so also our knowledge of the action of other bodies, such as alcoholic drinks, bitter principles, spices, etc., on the natural digestion is very uncertain and imper- fect. The difficulties which stand in the way of this kind of in- vestigation are very great, and therefore the results obtained thus far are often ambiguous or conflict with each other. For example, one investigator has seen that small quantities of alcohol or alcoholic drinks do not prevent but rather facilitate digestion ; others observe only a preventive action ; while other investigators believe DIGESTION. 191 to have found that the alcohol first acts somewhat as a preventive, but afterwards, as a rule, it is absorbed, producing an abundant secretion of gastric juice, and thereby facilitating digestion (CL. BERNARD, GLUZINSKI, CHITTENDEN). The digestion of sundry foods is not dependent on one organ alone, but divided among several. For this reason it is to be ex- pected that the various digestive organs can act for one another to a certain point, and that therefore the work of the stomach could be taken up more or less by the intestines. This in fact is the case. Thus the stomach of a dog has been almost completely extirpated (CZERNY), arid even that part necessary in the digestive process has been eliminated by plugging the pyloric opening (LuD- WIG and OGATA), and in both cases it was possible to keep the animal alive, well fed, and strong. In these cases it is evident that the digestive work of the stomach was taken up by the intestines. That the stomach nevertheless, during normal conditions, bears an essential part of the process of digestion may be inferred from the fact that the products of the proteolyse can generally be detected in the contents of the human stomach even shortly after a meal. By tests on dogs that had been given meat-powder, CAHN" found large quantities of peptone in the stomach, and this absorption, as shown by SCHMIDT-MULHEIM, requires about the same steps as digestion. It is meanwhile quite generally assumed that no peptonization of the proteids worth mentioning occurs in the stomach, and that the albuminous foods are only prepared in the stomach for the real digestive processes in the intestines. That the stomach serves in the first place as a storeroom follows from its shape, and this func- tion is of special value in certain new-born animals, for instance in dogs and cats. In these animals the secretion of the stomach con- tains only hydrochloric acid but no pepsin, and the casein of the milk is converted by the acid alone into solid lumps or a solid coagulum which fills the stomach. Small portions of this coagulum pass into the intestines only little by little and an overburdening of the intestines is thus prevented. In other animals, such as the snake and certain fishes, which swallow their food entire, it is certain that the major part of the process of digestion takes place in the stomach. The importance of the stomach in digestion cannot at once be de- cided. It varies for different animals, and it may vary in the same animal, depending upon the division of the food, the rapidity 192 PHYSIOLOGICAL CHEMISTRY. with which the peptonization takes place, the more or less rapid increase in the amount of hydrochloric acid, and so on. It is a well-known fact that the contents of the stomach may be kept without decomposing for some time by means of hydro- chloric acid, while, on the contrary, when the acid is neutralized a fermentation commences by which lactic acid and other organic acids are formed. The hydrochloric acid of the gastric juice has unquestionably an anti-fermentive action, and also, like dilute mineral acids, an antiseptic action. This action is of importance, as many disease micro-organisms may be destroyed by the gastric juice. The bacillus of cholera is killed by the normal acid gastric juice, while if it is introduced into the stomach after an injection of a soda solution it may remain active (KocH, NICATI and KIETSCH). Also varieties of streptococcus infecting wounds and the staphylococcus pyog. aureus are killed by the acid gastric juice (ALAPY). Still the gastric juice does not act on all micro-organisms, and especially in the state of spores they can withstand its action. As an example, the tubercle-virus is not destroyed by the gastric juice (FALK), and the spores of the anthrax bacteria do not seem to be always destroyed by the hydrochloric acid of the gastric juice. After death, if the stomach still contains food, digestion goes on of itself not only in the stomach but also in the neighboring organs during the slow cooling of the body. This leads to the question, why does the stomach not digest itself during life? Ever since PAVY has shown that after tying the smaller blood- vessels of the stomach of dogs the corresponding part of the mucous membrane was digested, efforts have been made to find the cause in the neutralization of the acid of the gastric juice by the alkali of the blood. That the reason for the non-digestion during life is to be sought for in the normal circulation of the blood cannot be contradicted; it is more probably found in the fact that the living mucous coat nourished by the alkaline blood shows quite different absorption, diffusion, and filtration properties than the dead mucous coat. This last was shown long ago by RANKE and HALENKE. Under pathological conditions irregularities in the secretion as well as in the absorption and in the mechanical work of the stomach may occur. Pepsin is almost always present, but the DIGESTION. 193 absence of the rennet enzyme, as above stated, may occur in many cases (BOAS, JOHNSON, KLEMPERER). In regard to the acid, it should be mentioned that sometimes this secretion may be in- creased so that an abnormally acid gastric juice is secreted, and sometimes may be decreased so that little or hardly any hydro- chloric acid is secreted. A hypersecretion of acid gastric juice sometimes occurs. In the secretion of too little hydrochloric acid the same conditions appear as after the neutralization of the acid contents of the stomach outside of the organism. Fermentation processes now appear in which, besides lactic acid, there appear also volatile fatty acids, such as butyric and acetic acids, etc., and gases like hydrogen. These fermentation products are therefore often found in the stomach in cases of chronic catarrh of the stomach, which may give rise to belching, heart-burn, and other symptoms. Among the foreign substances found in the contents of the stomach we have UREA, or ammonium carbonate derived there- from in uraemia; BLOOD, which generally forms a dark-brown mass through the presence of haematin, due to the action of the gastric juice ; BILE, which, especially during vomiting, easily finds its way through the pylorus into the stomach, but whose presence seems to be without importance. If it is desired to test the gastric juice or the contents of the stomach for pepsin, fibrin may be employed. If this is thoroughly washed immediately after beating the blood, well pressed and placed in glycerin, it may be kept in serviceable condition an inde- finitely long time. The gastric juice or the matter contained in the stomach — the latter, if necessary, having been previously diluted with 1 p. m. hydrochloric acid, is filtered and tested with fibrin at ordinary temperature. (It must not be forgotten that a control test must be made with acid alone and another porton of the same fibrin.) If the fibrin is not noticeably digested within one or two hours, no pepsin is present, or at most there are only slight traces. In testing for rennet enzyme the liquid must be first carefully neutralized. To 10 cc. unboiled amphoteric (not acid) reacting cow's milk add 1-2 cc. of the filtered neutralized liquid; but care must be taken not to add too much of the liquid from the stomach, for the coagulation may be retarded or prevented by diluting the milk. In the presence of rennet the milk should coagulate to a solid mass at the temperature of the body in the course of 10-20 minutes without changing its reaction. If the milk is diluted too much by the addition of the liquid of the stomach, only coarse 194 PHYSIOLOGICAL CHEMISTRY. flakes are obtained and no solid coagulum. Addition of lime salts is to be avoided, as they in great excess may produce a partial coagulation even in the absence of rennet. In many cases it is especially important to determine the degree of acidity of the gastric juice. This may be done by the ordinary titration methods. Phenol phthalein must not be used as an indicator, for we get too high results in the presence of large quantities of albumin. Good results may be obtained, on the con- trary, by using very delicate litmus-paper. As the acid reaction of the contents of the stomach may be caused simultaneously by several acids, still the degree of acidity is here, as in other cases expressed in only one acid, e. g., HC1. Generally the acidity is ~\t expressed by the number of cc. of — caustic soda which is required to neutralize the several acids in 100 cc. of the liquid of the stomach. An acidity of 43$ means that 100 cc. of the liquid of the stomach required 43 cc. of — caustic soda to neutralize it. It is also important to be able to ascertain the nature of the acid or acids occurring in the contents of the stomach. For this pur- pose, and especially for the detection of free hydrochloric acid, a great number of color reactions have been proposed, which are all based upon the fact that the coloring substance gives a characteristic color with very small quantities of hydrochloric acid, while lactic acid and the other organic acids do not give these colorations, or only in a certain concentration, which can hardly' exist in the con- tents of the stomach. These reagents are a mixture of FERRIC ACETATE and POTASSIUM SULPHOCYANIDE solution (MoHR's re- agent is modified by several investigators), METHYL, ANILIN- VIOLET, TROP^OLIU 00, CONGO RED, MALACHITE-GREEN, PHLO- ROGLUCIN-YANILLIN, BENZOPURPURIN 6 B, and others. As reagents for free lactic acid UFFELMANN suggests a strongly diluted, amethyst-blue solution of FERRIC CHLORIDE and CARBOLIC ACID or a strongly diluted, nearly colorless solution of FERRIC CHLORIDE. These give a yellow with lactic acid, but not with hydrochloric acid or with volatile fatty acids. The value of these reagents in testing for free hydrochloric acid or lactic acid is still disputed. Among the reagents for free hydro- chloric acid, MOHR'S test (even though not very delicate), GUNZ- BURG'S test with phloroglucin-vanillin, and the test with tro- paeolin 00, performed in moderate heat as suggested by BOAS, seem to be the most valuable. If these tests give positive results, then the presence of hydrochloric acid may be considered as proved. A negative result does not eliminate the presence of hydrochloric acid, as the delicacy of these reactions has a limit, and also the simul- DIGESTION. 195 taneous presence of albumin, peptones and other bodies influence the reactions more or less. The reactions for lactic acid may also give negative results in the presence of comparatively large quantities of hydrochloric acid in the liquid to be tested. Sugar and other bodies (FR. MULLER) may act with these reagents similarly to lactic acid. As the above-mentioned reactions for hydrochloric acid and organic acids are not claimed to be sufficient in exact investigations, while they may serve in many cases for clinical purposes, it will suffice to refer the reader to other text-books and especially to " Klinische Diagnostik innerer Krankheiten," by R. v. JAKSCH, 2d edition, 1889, for the performance and the relative value of these tests. The method suggested by CAHN and v. MEHRING for the detection and simultaneous quantitative estimation of hydrochloric acid in the presence of lactic acid and volatile fatty acids in the contents of the stomach will be here given. The chief points are the following: First the volatile acids are distilled and their quantity determined in the distillate by titration. The lactic acid is removed from the liquid remaining in the retort by repeated shaking with large quantities of ether, and after the evaporation of the ether the quantity of lactic acid in the residue is deter- mined by titration. The liquid which has been shaken out with the ether is either directly titrated for hydrochloric acid, or, as suggested by RABUTEAU, combines the hydrochloric acid with cin- chonin by digestion therewith at a gentle heat until the reaction is neutral. The cinchonin combination is then agitated with chloroform and the chloroform evaporated, and from the amount of chlorine in this residue we may calculate the quantity of pre- viously-free hydrochloric acid. This somewhat expensive and lengthy method is still not free from errors, and it is made useless by that following. The method of K. MORITER and SJOQVIST depends on the fol- lowing principle : When the gastric juice is evaporated to dryness with barium carbonate and then calcined the organic acids burn up and give insoluble barium carbonate, while the hydrochloric acid forms soluble barium chloride. From the quantity of this the original amount of hydrochloric acid can be calculated. 10 cc. of the filtered contents of the stomach is mixed in a small platinum or silver dish, by means of a clean, sharp knife-point, witli the barium carbonate free from chlorides, and evaporated to dryness. The residue is burnt and allowed to glow for a few minutes. The cooled carbon is gently rubbed with water and completely extracted with boiling water, and the filtrate (about 50 cc.) treated with an equal volume of alcohol and 3-4 cc. sodium acetate solution (10.^ acetic acid and 10# acetate). The amount of barium in the filtrate is determined by titration with a solution of potassium bichromate, 196 PHYSIOLOGICAL CHEMISTRY: in which the alcohol facilitates the precipitation of the barium chromate, while the acetate prevents in part the precipitation of the calcium carbonate and in part the setting free of hydrochloric acid. The potassium-bichromate solution should contain about 8.5 grms. potassium dichromate to the litre. Its titre must exactly JSJ correspond with an — barium-chloride solution, and the procedure is the same as in the titration of the BaCl2 solution obtained from the contents of the stomach. A paper moistened with tetramethyl- paraphenylendiamin is used as indicator; this is colored blue by a bichromate in acetic-acid solution. In titrating we add chromate solution as long as the barium chromate precipitated does not ap- parently increase, then test with the indicator paper after each addi- tion until it gives a decided blue coloration within one minute, and stop adding chromate solution. As the titre of the chromate solution has been determined by an — BaCl2 solution, it is easy to calculate the quantity of HC1 in 10 cc. of the gastric juice corresponding to the number of cc. of the chromate solution used. If the total acidity is determined in a second portion of the gastric juice, then the quantity of lactic acid or other organic acids represented as HOI may be calculated. This method, which is the most exact known at present, gives not only the amount of free hydrochloric acid, but also the hydrochloric acid combined with the albumin and peptone. In testing for volatile fatty acids the contents of the stomach should not be directly distilled, as volatile fatty acids may be formed by the decomposition of other bodies, such as albumin and haemoglobin. The neutralized contents of the stomach are there- fore precipitated with alcohol at ordinary temperature, filtered quickly, pressed, and repeatedly extracted by alcohol. The alcoholic extracts are made faintly alkaline by soda, and the alcohol distilled. The residue is now acidified by sulphuric or phosphoric acid and distilled. The distillate is neutralized by soda and evapo- rated to dryness on the water-bath. The residue is extracted witji absolute alcohol, filtered, the alcohol distilled off, and this residue dissolved in a little water. This solution may be directly tested for acetic acid with sulphuric acid and alcohol or with ferric chlo- ride. Formic acid may be tested for by silver nitrate, which quickly gives a black precipitate; and butyric acid is detected by the odor after the addition of an acid. In regard to the methods for more fully investigating the different volatile fatty acids, the reader is referred to other text-books. DIGESTION. 197 III. The Glands of the Mucous Membrane of the Intes- tines and their Secretions. The Secretion of Brunner's Glands. These glands are partly considered as small pancreas glands and partly as mucous or salivary glands, but according to GRUTZNER they are related to the pyloric iglands. They are assumed to contain pepsin (GRUTZNER and diastatic enzymes (COSTA, BUDGE and KROLOW). The diffi- culty of collecting the secretions of these glands free from contami- nation, however, makes these assumptions somewhat unreliable. The Secretion of Lieberkuhn's Glands. The secretion of these glands has been studied by the aid of a fistula in the intestines according to the method of THIRY and VELLA. Very little if any secretion takes peace in fasting animals (dog) when the mucous membrane is not irritated. The secretion begins in the first hour .after partaking of food, but the maximum varies with the quantity and character of the food (HEIDEKHAI^). Mechanical, chemical, or electrical irritation excites a secretion or increases that already begun (THIRY). Laxatives do not increase the secretion, while pilocarpin produces a very abundant one (MASLOFF and VELLA). The quantity of this secretion in the course of 24 hours has not been exactly determined. In the upper part of the small intestines of the dog this secre- tion is scanty, slimy, and gelatinous; in the lower part it is more fluid, with gelatinous lumps or flakes (ROHMANN). Intestinal juice has a strong alkaline reaction, generates carbon dioxide on the addition of an acid, and contains (in dogs) nearly a constant quantity of NaCl and Na2C03, 4.8-5 and 4.5 p. m. respectively (GuMiLEWSKi, ROHMAITN). It contains albumina (THIRY found 8.01 p. m.), the quantity decreasing with the duration of the elimi- nation. The quantity of solids varies. In dogs the quantity of solids is 12.2-24. 1 p. m., and in sheep 46-47 p. m. The specific gravity of the intestinal juice of the dog, according to the observa- tions of THIRY, is 1.010-1.0107. The action of intestinal juice has not been exhaustively studied and the opinions concerning it are somewhat at variance. According to most investigators, starch is converted into sugar, a statement 198 PHYSIOLOGICAL CHEMISTRY. which has lately been confirmed by BASTIANELLI. Intestinal juice or an infusion of the mucous coat inverts cane-sugar (PASCHUTLN, BASTIANELLI), and this inversion is, according to BEOW^ and HERON, produced even more quickly by the mucous membrane itself. Maltose seems to be quickly changed into glucose, and this action depends especially on PETER'S group of glands. The action on carbohydrates takes place more quickly and in greater quantity in the upper part of the intestine, and correspondingly the absorp- tion of starch and sugar occurs more quickly in the upper part than in the lower section of the intestine (LANHOIS and LEPINE, KOHMAFN). Intestinal juice does not split neutral fats, but it has the property, like other alkaline fluids, of emulsifying the fats. In regard to its action on albuminous bodies most investigators agree that the intestinal juice has no action on boiled albumin or flesh, while it dissolves fibrin according to THIBY. Albumoses are not converted into peptones (WENZ, BASTIANELLI). Contrary to other investigators, SCHIFF claims that the juice from a successful fistula, operation digests not only coagulated albumin and lumps of casein, but also unboiled and boiled flesh. The lack of action on albumins, which was observed by other investigators, SCHIFF attributes to the abnormal juice with which they experimented. SCHIFF also ob- tained from an operation not entirely successful a juice whose action on albumin and meat was no greater than that studied by THIRY and other investigators. Human intestinal juice in a case of anus prceternaturalis has been investigated by DEMANT. This juice showed itself entirely inactive on albuminous bodies, even on fibrin and on fats. It only showed a very faint action on boiled starch. Those tests on the action of the intestinal juice which are made, in isolated stoppage of the intestine in animals or human intestine in cases of anus prceternaturalis, on the food which has been introduced into the in- testine, generally give no positive results, because of the putrefac- tion processes going on in the intestine. The secretion of the glands in the large intestine seems to con- sist chiefly of mucus. Fistulas have also been introduced into these parts of the intestine, which are chiefly if not entirely to be considered as absorption organs. The investigations on the action of DIGESTION. 199 this secretion on nutritive bodies have not as yet yielded any positive results. IV. Pancreas Gland and Pancreatic Juice. In invertebrata, which have no pepsin digestion and which also have no formation of bile, the pancreas, or at least an analogous organ, seems to be the essential digestion gland. On the contrary, an anatomically characteristic pancreas is absent in certain vertebrata and in certain fishes. Those functions which should be performed by this organ seem to be performed in these animals by the liver, which may be rightly called HEPATOPANCREAS. In man and in most vertebrata the formation of bile and of certain secretions containing enzymes important for digestion is divided between the two organs, the liver and the pancreas. The pancreas gland is similar in certain respects to the parotid gland. The elements secreted by the former consist of nucleated cells whose basis forms a mass rich in albumin, expand in water and in which two distinct zones exist. The outer zone is more homogeneous, the inner cloudy due to a quantity of granular, substance. The nucleus lies about midway between the two zones, but this position may change with the varying relative size of the two zones. According to HEIDENHAIN, the inner part of the cells diminishes in size during the first stages of digestion, in which the secretion is active, while at the same time the outer zone enlarges owing to the absorption of new material. In a later stage, when the secretion has decreased and the absorption of the nutritive bodies has taken place, the inner zone enlarges at the expense of the outer, the substance of the latter having been converted into that of the former. Under physiological conditions the glands are undergoing a constant change, at one time consuming from the inner part and at another time growing from the outer part. The inner granular zone is converted into the secretion, and the outer, more homogeneous zone, which contains the repairing material, is then converted into the granular substance. Besides considerable quantities of proteids, globulin, nucleo- albumin, and albumin, we find in this gland three enzymes, or, 200 PHYSIOLOGICAL CHEMISTRY. more correctly, three zymogens, which will be described later. We also find in this gland nuclei n, leucin (butalanin), tyrosin (not in the perfectly fresh gland), xanthin, 1-8 p. m., hypoxanthin, 3-4 p. m., guanin, 2-7.5 p. m. (all figures are calculated for the dried substance, KOSSEL), adenin, inosit, lactic acid, volatile fatty acids, fats, and mineral substances. According to the investigations of OIDTMAN, the human pancreas contains 750-760 p. m. water, 240- 250 p. m. organic and 3.7-9.5 p. m. inorganic substances. Pancreatic Juice. This secretion may be obtained by adjusting a fistula in the excretory duct, according to the methods suggested by BERNARD, LUDWIG, and HEIDE^HAIN. If the operation is performed with sufficient rapidity and dexterity on an animal which has been well fed a few hours before, there is obtained from the fistula, as a rule, immediately after the operation (temporary fistula) a secretion rich in solids, viscid, very active, and which may be considered as normal pancreatic juice. Ordinarily, however, the gland becomes diseased in a few hours or days after the operation, and the secretion which then flows out of the fistula (permanent fistula) is more liquid, deficient in solids, and in certain other respects different from the secretion obtained immediately after the operation. Still a permanent fistula may also sometimes yield a normal secretion for a long time (HEIDENHAIK), while the tem- porary fistula in careless operations may give no secretion or only an abnormal juice. In herbivora, such as rabbits, whose digestion is uninterrupted, the secretion of the pancreatic juice is continuous. In carnivora it seems, on the contrary, to be intermittent and dependent on the digestion. During starvation the secretion almost stops, but com- mences again after partaking of food. Food seems to act in a two- fold manner. First, it may, with the more abundant flow of blood during the digestion, which is seen by the red color of the gland, convey a larger quantity of nutritive material to the gland, and thereby cause the secretion of a juice rich in solid nutritive bodies. In another way the food may also, by the irritation which it pro- duces on the mucous coat of the stomach and the duodenum, cause an increased secretion. That the food indeed acts in these two ways follows from the fact that other substances, such as ether, may also cause a secretion of pancreatic juice, but by this means DIGESTION. 201 in starvation a thin fluid is secreted, and after partaking of food a thick fluid is produced. According to the observations of BERNSTEIN, HEIDENHAIN, and others, the secretion increases rapidly after eating, and it reaches its maximum in the cours^pf the first three hours. From this time the secretion diminishes, but may again increase from the 5th-7th hour, when generally large quantities of food pass from the stomach to the intestine. Then it again decreases continuously from the 9th-llth hour, and stops entirely at the 15th-16th hour. The statements as to the amount of pancreatic juice secreted in the course of 24 hours are variable and not trustworthy. It seems positively proved that the permanent fistula yields a con- siderably larger quantity of secretion than the temporary. While KEFERSTEIN and HALLWACHS, and SCHMIDT and KROGER, find that the quantity of juice secreted from the first is 45-100 grms. per kilo during 24 hours, BIDDER and SCHMIDT and BIDDER and SKREBITZKY claim that the quantity from the temporary fistula is 2.5-5 grms. per kilo in the same time. In regard to the constituents and composition of the pancreatic juice, a distinction must be made between the secretion of a tem- porary and of a permanent fistula. The secretion flowing from the former is in dogs a clear, colorless, n early-si rupy, odorless fluid of an alkaline reaction which is very rich in albumin and sometimes containing so large a quantity that it coagulates like white of egg when heated. Besides albumin the juice contains also three- en- zymes— one diastatic, one fat-splitting, and one which dissolves proteids. The last-mentioned has been called trypsin by KUHNE. Besides the above-mentioned bodies the pancreatic juice habitually contains small quantities of leucin, fat, and soaps. As mineral constituents it contains chiefly alkali chlorides, also alkali carbo- nates, and some phosphoric acid, lime, magnesia, and iron. The secretion from the permanent fistula always contains less solids, especially albumin and enzymes, than that from a tempo- rary fistula. A long time after the operation it is more fluid, more strongly alkaline, and the property which the juice from the tem- porary fistula has of dissolving albumin is often absent, or the secretion shows it in only a slight degree. As an example of the different composition of the juice from a temporary and from a 202 PHYSIOLOGICAL CHEMISTRY. permanent fistula, we give below the analyses of C. SCHMIDT. The figures represent parts per 1000. Juice from a Temporary Juice from a Permanent Fistula. Fistula. A . a. b. a. b. c. Water 900.8 884.4 976.8 979.9 984.6 Solids 99.2 115.6 23.2 20.1 15.4 Organic substance ... 90.4 16.4 12.4 9.2 Ash 8.8 6.8 7.5 6.1 The mineral constituents of the secretion from the temporary fistula con- sisted chiefly of NaCl, 7.4 p. m. In the pancreatic juice of rabbits 11-26 p. m. solids have been found, and in that from sheep 14.3-36.9 p. m. In the pancreatic juice of the horse 9-17.5 p. m. solids have been found ; in that of the pigeon, 12-14 p. m. The human pancreatic juice has been analyzed by HERTER in a case of stoppage of the exit of the juice by the pressure of a cancer. This juice, which could hardly be considered as normal, was clear, alkaliue, without odor, and contained the three enzymes. It contained peptone, but no other pro- teid. The quantity of solids was 24.1 p. m. Of these 6.4 p. m. were soluble in alcohol. It contained 11.5 p. m. peptones (and enzymes) and 6.2 p. m. mineral substances. Among the constituents of the pancreatic juice, the three enzymes are the most important. The pancreatic diastase, which according to KOROWIN and ZWEIFEL is not found in new-born infants and does not appear until more than one month after birth, seems, although not identi- cal with ptyalin, to be nearly related to it. The pancreatic diastase acts very energetically upon boiled starch, especially at -j- 37° to 40° C., and besides dextrin yields maltose with only a small quantity of glucose (MUSCULUS and v. MERING). If the natural pancreatic juice is not to be obtained, then the gland, best after it has been exposed a certain time (24 hours) in the air, may be treated with water or glycerin. This infusion or the glycerin extract diluted with water (when a glycerin has been used which has no reducing action) may be tested directly with starch-paste. It is safer, however, to first precipitate the enzyme from the glycerin extract by alcohol, and wash with this liquid, dry the precipitate over sulphuric acid, and extract with water. The enzyme is dissolved by the water. The detection of sugar may be made in the same manner as in the saliva. The fat-splitting enzyme. The action of the pancreatic juice on fats is twofold. First, the neutral fats are split into fatty acids and glycerin, which is an enzymotic process; and secondly, it has also the property of emulsifying the fats. DIGESTION. 203 The action of the pancreatic juice in splitting the fats may be shown in the following way. Shake olive-oil with caustic soda and ether, siphon off the ether and filter if necessary, then shake the ether repeatedly with water and evaporate at a gentle heat. In this way we obtain a residue of fat free from fatty acids which is com- pletely neutral, and which dissolves in acid-free alcohol and is not colored red by alkanet tincture. If such fat is mixed with quite fresh alkaline pancreatic juice or with a freshly-prepared infusion of the fresh gland and treated with a little alkali or with a faintly alkaline glycerin extract of the fresh gland (9 parts glycerin and 1 part soda solution of \% for each gramme of the gland) and some litmus tincture added and the mixture warmed to -|- 37° C., the alkaline reaction will gradually disappear and an acid one takes its place. This acid reaction depends upon the conversion of the neutral fats by the enzyme into glycerin and free fatty acids. The splitting of the neutral fats may also be shown more exactly by the following method. The mixture of neutral fats (absolutely free from fatty acids) and pancreatic juice or pancreas infusion is digested at the temperature of the body and treated with some soda and repeatedly shaken with fresh quantities of ether until all the unsplit neutral fats are removed. Then it is made acid with sulphuric acid, after which shake the acid liquid with ether, evapo- rate the ether, and test the residue for fatty acids. Another simple process for the demonstration of the fat-splitting action of the pancreas glands is the following (CL. BERNARD). A small portion of the perfectly-fresh, finely-divided gland substance is first soaked in alcohol (of 90$). Then the alcohol is removed as far as possible by pressing between blotting-paper, after which the pieces of gland are covered with an ethereal solution of neutral butter-fat (which may be obtained by shaking milk with caustic soda and ether). After the evaporation of the ether the pieces of gland covered with butter-fat are pressed between two watch-glasses and then gently heated to 37° to 40° C. in this position. After a certain time a marked odor of butyric acid appears. The action of the pancreatic juice in splitting fats is a process analogous to that of saponification, the neutral fats being decom- posed, by the addition of the elements of water, into fatty acids and glycerin according to the following formula: CsH^Os-Rs (neutral fat) + 3H20 = C3H5.03.H3 (glycerin) + 3(H.O.R) (fatty acid). This depends upon a hydrolytic splitting, which was first positively proved by BERNARD and BERTHELOT. The pancreas enzyme alsa decomposes other esters just as it does the neutral fats (NENCKi). The pancreas-enzyme which decomposes fats has been less studied 204 PHYSIOLOGICAL CHEMISTRY. than the other pancreas-enzymes, and it has indeed been questioned whether or not the decomposition of the neutral fats in the intestine may not be effected through lower organisms. According to the investigations of NENCKI, it seems that the pancreas actually con- tains an enzyme which decomposes fats. This enzyme, which is still little known, appears to be very sensitive to acids, and it is often absent in acid glands not perfectly fresh. If a watery in- fusion of the gland prepared cold be treated with calcined magnesia, then the enzyme in question will, according to DANILEWSKI, be retained by the magnesia precipitate. The fatty acids which are split off by the action of the pan- creatic juice combine in the intestine with the alkalies, forming soaps which have a strong emulsifying action on the fats, and thus the pancreatic juice becomes of great importance in the emul- gtification and the absorption of the fats. Trypsin. The action of the pancreatic juice in digesting pro- teids was first observed by BERNARD, but first proved by CORVISART. It depends upon a special enzyme called by KUHNE trypsin. Strictly speaking, this enzyme does not occur in the gland itself. In the gland, more probably, a zymogen occurs from which the enzyme is split off or formed by the secretion, also by the action of water, acids, alcohol, and other substances. According to ALBER- TONI, this zymogen is found in the gland in the last third of the intra-uterine life. The purest trypsin thus far prepared, isolated by KUHNE, is soluble in water but insoluble in alcohol or glycerin. The less pure enzyme, on the contrary, is soluble in glycerin. If the solution of the enzyme in water is heated to the boiling point with the addi- tion of a little acid, it decomposes into coagulated albumin and peptone (KiJHNE). It is destroyed by gastric juice. Like other enzymes, trypsin is characterized by its physiological action. This action consists in its power of dissolving albumin, and especially easily fibrin, in alkaline, neutral, and even in faintly acid-reacting solutions. The production of pure trypsin has been tried by various ex- perimenters, DANILEWSKI, HUFNER, KUHNE, LOEW, and others. The purest seems to have been prepared according to the rather com- plicated method of KUHNE. In studying the action of trypsin a 205 Jess pure preparation may often answer, and various methods of preparing such have been proposed, but we cannot describe all of them. For the production of a glycerin extract the gland should be rubbed with glass powder or pure quartz-sand, this mass care- fully mixed with acetic acid of \% (1 cc. to each grm. of gland), then for each part of the gland-mass add 10 parts of glycerin, and filter after about three days. By precipitating the glycerin extract with alcohol and redissolving the precipitate in water, we obtain a solution which has a powerful digestive action (HEIDENHAIN). A watery infusion of the gland may be made only after it has been exposed to the air for 24 hours, and 5-10 parts of water for each part by weight of the gland should be used. The simplest method is to cut the gland fine and place it in a flask and allow it to digest with water to which 5—10 cc. of chloroform (SALKOWSKI) or ether has been added for each litre. After a few days we obtain in this way a powerful infusion which keeps. The action of trypsin on proteids is best demonstrated by the use of fibrin. Very considerable quantities of this albuminous body are dissolved by a small amount of trypsin at 37°-40° C. It is always necessary to make a control test with fibrin alone, with or without the addition of alkali. Fibrin is dissolved by trypsin without any putrefaction; the liquid has a pleasant odor somewhat like bouillon. To completely exclude putrefaction a little thymol, chloroform, or ether should be added to the liquid. Trypsin digestion differs essentially from pepsin digestion in that the first takes place in neutral or alkaline reaction and not, as is necessary for pepsin di- gestion, in an acidity of 1-2 p. m. HOI, and further by the fact that the proteids dissolve in the trypsin digestion without previous ex- pansion. Many circumstances exert a marked influence on the rapidity of the trypsin digestion. With an increase in the quantity of enzyme present the digestion is hastened at least to a certain point, and the same is also true of an increase in temperature at least to about -f 40° C., at which temperature the albumin is very rapidly dissolved by the trypsin. The reaction is also of the greatest im- portance. Trypsin acts energetically in neutral, or still better in alkaline, solutions, and best in an alkalinity of 3-4 p. m. Na^O^ Free mineral acids, even in very small quantities, completely prevent the digestion. If the acid is not actually free, but combined with albuminous bodies, then the digestion may take place quickly when 206 PHYSIOLOGICAL CHEMISTRY. the acid combination is not in too great excess (CHITTENDEN and CUMMINS). Organic acids act less disturbingly, and in the presence of 0.2 p. m. lactic acid and the simultaneous presence of proteid, bile, and common salt the digestion may indeed proceed more quickly than in a faintly-alkaline liquid (LINDBERGER). Foreign bodies, such as borax and potassium cyanide, may promote digestion, while bodies such as mercuric, fenic and other salts (CHITTENDEN and CUMMINS), or salicylic acid in large quantities (KUHNE), may act preventively. The nature of the proteids is also of importance. Unboiled fibrin is, relatively to most other albuminous bodies, dis- solved so very quickly that the digestion test with raw fibrin gives an incorrect idea of the power of trypsin to dissolve coagulated albuminous bodies in general. An accumulation of digestion pro- ducts tends to hinder the trypsin digestion. The Products of the Trypsin Digestion. In the digestion of un- boiled fibrin a globulin which coagulates at -f- 55°-56° C. may be obtained as an intermediate product (HERRMANN). Moreover from fibrin, as well as from other albuminous bodies, albumoses and peptones, leucin, tyrosin and aspartic acid, a little ammonia (HIRSCHLER), and a substance whose nature is not known and which gives a beautiful purple-red liquid with chlorine or bromine water in acid solution, have been obtained. When putrefaction has not been entirely prevented numerous other bodies appear which will be spoken of later in connection with the putrefaction process going on in the intestine. In the trypsin digestion, in contrast to the pepsin digestion, pure peptones, not precipitated by ammonium sul- phate, are relatively easily and quickly formed. The peptone, according to KUHNE consists entirely of antipeptone, and the above- mentioned decomposition products, leucin and the others, are formed by the decomposition of the hemipeptone. We will now consider leucin and tyrosin decomposition products formed by the trypsin digestion of proteids. Leucin, C6H13M)2, or AMIDO-CAPROIC ACID, C5H10(NH2)COOH, besides occurring in the trypsin digestion of proteids, is derived from the protein substances by their decomposition on boiling with diluted acids or alkalies, by melting with alkali hydrates, and by putrefaction. Because of the ease with which leucin and tyrosin are formed in the decomposition of protein substances, it is difficult DIGESTION. 207 to positively decide whether these bodies when found in the tissues are constituents of the living body or are only to be considered as decomposition products formed after death. Leucin has been found in the pancreas and its secretion, in the spleen, thymus and lymph- glands, in the thyroid gland, in the salivary glands, in the kidneys, brain, and liver (but mostly in disease). It also occurs in the wool of sheep, in dirt from the skin (inactive epidermis) and between the toes, and its decomposition products have the disagreeable odor of the perspiration of the feet. It is found pathologically in atheromatous ulcers, ichthyosis scales, pus, blood, and urine (in dis- eases of the liver). Leucin also occurs in the vegetable kingdom. Leucin may be prepared synthetically most simply by the action of ammonia on monobrom-caproic acid (HUFNER). On heating with fuming hydriodic acid to 140° C. it splits into ammonia and caproic acid. On heating leucin alone it decomposes with the for- mation of carbon dioxide, ammonia, and amylamin. On fusing with caustic alkali, as also on putrefaction, it yields valerianic acid and ammonia. Leucin crystallizes when pure in shining, white, very thin plates, usually forming round knobs or balls, either appearing like hyalin or alternating light or dark concentric layers which consist of radial groups of crystals. Leucin as obtained from the animal fluids and tissues is very easily soluble in water and rather easily in alcohol. Pure leucin is soluble with difficulty; it dissolves in 27 parts cold water, in 1040 parts cold and in 800 parts boiling alco- hol. It is easily dissolved by alkalies and acids. On slowly heating to 170° C. it melts and sublimes in white, woolly flakes which are similar to sublimed zinc oxide. A marked odor of amylamin is generated at the same time. The solution of leucin in water is not, as a rule, precipitated by metallic salts. The boiling-hot solution may, however, be precipi- tated by a boiling-hot solution of copper acetate. If the solution of leucin is boiled with sugar of lead and then ammonia be added to the cooled solution, shining crystalline leaves of leucin-lead oxide separate. "When boiled with leucin, copper oxyhydrate is dissolved without reduction. Leucin is recognized by the appearance of the balls or knobs under the microscope, by its action when heated (sublimation test), 208 PHYSIOLOGICAL CHEMISTRY. and by SCHERER'S test. This last consists in the leucin yielding a colorless residue when carefully evaporated with nitric acid on platinum-foil, and this residue when warmed with a few drops of caustic soda gives a color varying from a pale yellow to brown (de- pending on the purity of the leucin), and on further concentrating over the flame it agglomerates into an oily drop which rolls about on the foil. Tyrosin, C9HUN03 , or p. OXYPHENYL-AMIDOPROPRIONIC ACID, HO.C6H4.C2H3(NH2).COOH, is derived from most protein sub- stances (not gelatin) under the same conditions as leucin, which it habitually accompanies. It is especially found with leucin in large quantities in old cheese (Tvpos), from which it derives its name. Tyrosin has not with certainty been found in perfectly fresh organs, with the exception, perhaps, of the spleen and pancreas of cattle. It occurs in the intestine in the digestion of albuminous sub- stances, and it has about the same physiological and pathological importance as leucin. Tyrosin was prepared by ERLENMEYER and LIPP from p. amido- phenylalanin by the action of nitrous acid. . On fusing with caustic alkali it yields p. oxybenzoic acid, acetic acid, and ammonia. By putrefaction it may yield p. hydrocoumaric acid, oxyphenyl-acetic acid, and p. cresol. Tyrosin in a very impure state may be in the form of balls similar to leucin. The purified tyrosin, on the contrary, appears as colorless, silky, fine needles which are often grouped into tufts or balls. It is soluble with difficulty in water, being dissolved by 2454 parts water at -j- 20° C. and 154 parts boiling water, separating, however, as tufts of needles on cooling. It dissolves more easily in the presence of alkalies, ammonia, or a mineral acid. It is difficultly soluble in acetic acid. Crystals of tyrosin separate from an ammoniacal solution on the spontaneous evaporation of the ammonia. It is not soluble in alcohol or ether. Tyrosin is known by its crystalline form and by the following reactions : PIRIA'S Test. Tyrosin is dissolved in concentrated sulphuric acid by the aid of heat, by which tyrosin sulphuric acid is formed; it is allowed to cool, diluted with water, neutralized by BaC03, and filtered. On the addition of a solution of ferric chloride, the filtrate gives a beautiful violet color. This reaction is impeded by DIGESTION. 209 the presence of free mineral acids and by the addition of too much ferric chloride. HOFMANN'S Test. If some water is poured on a small quantity of tyrosin in a test-tube and a few drops of MILLON'S reagent added and then the mixture boiled for a certain time, the liquid becomes a beautiful red and then yields a red precipitate. Or mercuric nitrate may first be added, then, after this has boiled, nitric acid which contains some nitrous acid. SCHERER'S Test. If tyrosin is carefully evaporated to dryness with nitric acid on platinum-foil, a beautiful yellow residue (nitro- tyrosin nitrate) is obtained, which gives a deep reddish-yellow color with caustic soda. This test is not characteristic, as other bodies give a similar reaction. Leucin and tyrosin may be prepared in large quantities by boiling albuminous bodies or albuminoids with dilute mineral acids. Ordinarily we boil hoof-shavings (2 parts) with dilute sul- phuric acid (5 parts concentrated acid and 13 parts water) for 24 hours. After boiling the solution it is diluted with water and neu- tralized while still warm with milk of lime and then filtered. The calcium sulphate is repeatedly boiled with water, and the several filtrates are united and concentrated. The lime is precipitated from the concentrated liquid by oxalic acid and the precipitate filtered off, repeatedly boiled with water, all filtrates united and evaporated to crystallization. What first crystallizes consists chiefly of tyrosin with only a little leucin. By concentration a new crystallization may be produced in the mother-liquor, which consists of leucin with some tyrosin. To separate leucin and tyrosin from each other their different solubilities in water may be taken advan- tage of in preparing them on a large scale, but surer and better results are obtained by the following method of HLASIWETZ and HABERMANIST. The crystalline mass is boiled with a large quantity of water and enough ammonia to dissolve it. To this boiling-hot solution enough basic lead acetate is added until the precipitate formed is nearly white; now filter, heat the light yellow filtrate to boiling, neutralize with sulphuric acid, and filter while boiling hot. After cooling, nearly all the tyrosin is precipitated, while the leuciu remains in the solution. The tyrosin may be purified by recrys- tallizing from boiling water or from ammoniacal water. The above-mentioned mother-liquor rich in leucin is treated with HgS, the filtrate concentrated and boiled with an excess of freshly-pre- cipitated copper oxyhydrate. A part of the leucin is precipitated, and the residue remains in the solution and partly crystallizes as a cuprous compound on cooling. The copper is removed from the 210 PHYSIOLOGICAL CHEMISTRY. precipitate and solution by means of H2S, the filtrate decolorized when necessary with animal charcoal, strongly concentrated and allowed to crystallize. The leucin obtained from the precipitate is quite pure, while that from the solution is somewhat contaminated. If one is working with small quantities, the crystals, which consist of a mixture of the two bodies, may be dissolved in water and this solution precipitated with basic lead acetate. The filtrate is treated with H2S, the new filtrate evaporated to dryness, and the residue treated with warm alcohol which dissolves the leucin but not the tyrosin. The remaining tyrosin is purified by recrystalliza- tion from ammoniacal alcohol. Leucin may be purified by recrys- tallization from boiling alcohol or by precipitating it as leucin lead oxide, treating the precipitate suspended in water with H2S and evaporating the filtered solution to crystallization. To detect the presence of leucin and tyrosin in animal fluids or tissues the albumin must first be removed by coagulation with the addition of acetic acid and then precipitated by basic lead acetate. The filtrate is treated with H2S, this filtrate evaporated to a syrup or to dryness, and the two bodies in the residue are separated from each other by boiling alcohol and then purified as above stated. Aspartic Acid, C4H7N04, or AMIDO-SUCCINIC ACID, C2H3(NH2). (COOH)2. This acid has been obtained in the trypsin digestion of fibrin (RADZIEJEWSKI and SALKOWSKI) and of glutin (v. KNIE- KIEM). It may also be obtained by the decomposition of albumin- ous bodies or albuminoids with acids (see Chap. II). It has also , been found in beet-root molasses, and lastly it is very widely dif- fused in the vegetable kingdom as the amid ASPARAGINE (amido- succinic-acid amid), which seems to be of the greatest importance in the development and formation of the albuminous bodies. Aspartic acid dissolves in boiling water and crystallizes on cool- ing into rhombic prisms. It is optically active, and is dextrogyrate in a solution strongly acid with nitric acid. It forms with copper oxide a crystalline combination which is soluble in boiling-hot water and nearly insoluble in cold water, and which may be used in the preparation of the pure acid from a mixture with other bodies. The action of trypsin on other bodies has not been thoroughly studied. An enzyme has been found in the pancreas-gland of the pig and certain herbivora which causes a coagulation of neutral or alkaline milk (KtiHNE and ROBERTS). Gelatin is dissolved by the pancreatic juice and is converted into gelatin peptone. In tests with a very impure infusion (self -digestion of the gland in the pres- DIGESTION. ence of gelatine), besides gelatin peptone NENCKI obtained leucin, glycocoll, ammonia, a base, C8HUN, and other products. The pure enzyme, according to KUHITE, gives neither glycocoll nor leucin with gelatin. The gelatin-giving substance of the connective tis- sues is not directly dissolved by trypsin, but only after it has been treated with acid or after soaking in water at -}- 70° C. By the action of trypsin on hyalin cartilage the cells dissolve, leaving the nucleus. The basis is softened and shows an indistinctly-con- structed network of collagenous substance (Kunins and EWALD). The elastic substance, the structureless membrane, and the mem- brane of the fat-cells are also dissolved. Parenchymatous organs, such as the liver and the muscles, are dissolved to the nucleus, and also connective tissue, fat-corpuscles, and the remainder of the nervous tissue. If the muscles are boiled, then the connective tissue is also dissolved. Trypsin seems to be without action on chitin and horny substance. Oxyhcemoglobin is decomposed by trypsin with the splitting off of haematin. Hcemoglobin, on the contrary, wh6n the access of oxygen is completely prevented, is not decom- posed by trypsin (HoppE-SEYLEE). Trypsin does not act on fats or carbohydrates. It was already brought out above that trypsin does not exist ready formed in the gland, but more likely, as HEIDENHAIN has shown, the gland contains a corresponding zymogen. The maxi- mum quantity of such zymogen in the gland occurs 14-16-18 hours after an abundant meal, and the minimum 6-10 hours after. This zymogen is not changed by glycerin so that it forms trypsin, but is easily changed by water and acids. A soda solution of 1-1.50, on the contrary, prevents almost entirely the changing of the zymogen. If we allow the gland to lie in the air it gradually becomes acid, and this leads to the formation of an enzyme in which the oxygen seems to be active, as is usual in the conversion of the zymogen into trypsin. It is very probable also that the two other enzymes are formed from corresponding zymogens, and this has been shown to be plausible in regard to the diastatic enzymes by LIVERSIDGE. After a plentiful meal HEIDENHAIN found in dogs in the first stages of digestion, when the secretion of pancreatic juice was most active, that the glandular cells became smaller owing to the 212 PHYSIOLOGICAL CHEMISTRY. consumption of the inner granular zone, while the outer zone at the same time took up new material and became larger. In these stages the quantity of zymogen is smallest. At a later period, 12-20 hours after a meal, the inner zone is re-formed from the outer, and the larger this zone is the larger the quantity of zymogen in the gland seems to be. The zymogen consequently belongs to the inner zone, and the secretion consists therefore, at least in part, in a destruc- tion or dissolution of this zone whereby the substance of the gland itself is changed into the secretion (HEIDENHAIN). This view, however, is in opposition to that of LEWASCHEW, who observed that in animals which have starved and whose pancreas-glands are nearly free from zymogen, the inner granular zone is just as much developed as under normal conditions and contains abundant quan- tities of zymogen. We are still completely in the dark regarding the nature of the chemical processes which take place in the con- version of the zymogen into the enzyme. V. The Chemical Processes in the Intestine. The action which belongs to each digestive secretion may be essentially changed by mixing with other digestive fluids; and since the digestive fluids which flow into the intestine are mixed with still another fluid, the bile, it will be readily understood that the combined action of all these fluids in the intestine makes the chemical processes going on therein very complicated. As the acid of the gastric juice acts destructively on ptyalin, this enzyme has no further diastatic action, even after the acid of the gastric juice has been neutralized in the intestine. The bile has, at least in certain animals, a faint diastatic action which in itself can hardly be of any great importance, but which shows that the bile has not a preventive but rather a beneficial influence on the energetic diastatic action of the pancreatic juice and the faint diastatic action of the intestinal juices. MAKTIK and WILLIAMS observed, in experiments made recently, a beneficial action of the bile on the diastatic action of the pancreas infusion. To this may be added that the organized ferments which occur habitually in the intestine and sometimes in the food have partly a diastatic DIGESTION. 213 action and partly produce a lactic-acid and butyric-acid fermen- tation. The maltose which is formed from the starch seems to be con- verted into glucose in the intestine. The cellulose, especially the finer and more tender, is undoubtedly partly dissolved in the intes- tine ; the products formed hereby are not very well known. It has been shown by TAPPENIER that cellulose may produce a marsh-gas fermentation in the intestines caused by the action of a micro- organism; but we do not know to what extent the cellulose is de- stroyed and what part is valueless for the organism. Bile possesses the power of dissolving fats in so slight a degree that it is scarcely worthy of mention. It is, however, without doubt of greater importance that the bile, as NENCKI has shown, facilitates the fat-splitting action of the pancreatic juice. This splitting of the fats into fatty acids and glycerin is an important factor in the absorption of the fats. The fatty acids combine with the alkalies of the bile and most readily with the alkalies of the intestinal and pancreatic juices, producing soaps which may be partly absorbed as such and partly exercise a powerful action on the absorption of the fats. There is no doubt that the chief part of the fats in the foods is absorbed as a fine emulsion, and for this reason the soaps are of such importance in the formation of this emulsion. If to a soda solution of about 2 p. m. N^COg we add pure, actually neutral olive-oil in not too large quantity, we obtain, after vigorous shaking, a transient emulsion. If, on the contrary, we add to the same quantity of soda solution an equal amount of com- mercial olive-oil (which always contains free fatty acids), we need only turn the vessel over for the two liquids to mix and immediately we have a very finely-divided and permanent emulsion making the liquid appear like milk. The free fatty acids of the always some- what rancid commercial oil combine with the alkali forming soaps, which act to emulsify the fats (BRUCKE, GAD). This emulsifying action of the fatty acids split off by the pancreatic juice is undoubt edly assisted by the habitual occurrence of free fatty acids in the food, and also by the splitting off of fatty acids from the neutral fats by the putrefaction in the intestine. These fatty acids must also combine with the alkalies in the intestine and form soaps. This emulsification of fats by means of the action of the pan- 214 PHYSIOLOGICAL CHEMISTRY. creatic juice or by soaps formed in other ways can only take place in an alkaline solution. In the contents of the intestine, as long as they are acid, such an emulsion can hardly occur. On the con- trary, it undoubtedly occurs at the point where the fat comes in contact with an alkaline secretion under a mucous membrane, or in general where it meets with sufficient alkali to form an emul- sion. In the acid contents of the intestines of dogs, which had been kept on food rich in fat, LUDWIG and CASH observed no emulsion. After tying the two pancreas excretory ducts they found a remarkably fine emulsion in the chylous vessels, though the fat in the contents of the intestine was not emulsified. In this case it is possible that the free fatty acid which is hardly ever absent in the fat of the food, and which may be produced also by putrefaction in the intestine, forms soaps with the alkali of the mucous coat of the intestine and produces the emulsion in the chylous vessels. CLAUDE BERNARD found long ago in his experiments on rab- bits, in which animals the choledochus duct to the small intestine was inosculated above the pancreas passages, that when their food contained a large proportion of fat the chylous vessels of the intes- tine above the pancreas passages were transparent, but below the same they were milky-white, and from this concluded that the bile alone, without the pancreatic juice, does not emulsify fats. DASTRE tried the reverse experiment in dogs, namely, tying the choledo- chus duct and producing a biliary fistula, through which the bile would flow into the intestine below the mouth of the pancreatic passages. When the animals were killed after a meal rich in fat, the chylous vessels were first milky-white below the opening of the biliary fistula. DASTRE draws the following conclusion from this : that combined action of the bile and the pancreatic juice is neces- sary for the absorption of the fats — a deduction which coincides with the above-mentioned observations of NENCKI. Bile has no solvent action on proteids, but still it may have an influence on their digestion. The acid contents of the stomach, containing an abundance of proteids, give with the bile a precipi- tate of proteids and bile-acids. This precipitate carries a part of the pepsin with it, and for this reason and on account of the partial or complete neutralization of the acid of the gastric juice by the alkali of the bile and the pancreatic juice the pepsin digestion can- DIGESTION. not proceed further in the intestine. On the contrary, the bile does not disturb the digestion of albumin by means of the pancre- atic juice in the intestine. The action of these digestive secre- tions, as above stated, is not disturbed by the bile, especially not by the faintly-acid reaction due to organic acids which are habitually found in the upper parts of the intestine. In a dog killed while digestion is going on, the faintly -acid, bile-containing matter of the intestine shows a strong digestive action on albumin. The precipitate formed on the mixing of the acid contents of the stomach and the bile dissolves easily — partly by the acid reac- tion— in an excess of the bile, also in the NaCl produced by the neutralization of the hydrochloric acid of the gastric juice. Since in man the exit passages of the bile and the pancreatic juice open near one another, and therefore the acid contents of the stomach are probably immediately neutralized by the bile as soon as it enters, it is doubtful whether a precipitation of albumin by the bile occurs in the intestine. Besides the previously-mentioned processes caused by enzymes, there are others of a different nature going on in the intestine, namely, the putrefaction processes caused by micro-organisms. These are less intense in the upper parts of the intestine, but increase in intensity towards the lower part of the same, and decrease in the large intestine because of the absorption of water. A positive proof that the micro-organisms are active in these processes lies in the fact that they occur very abundantly in the contents of the intestine; and it is to be remarked that these organisms occur in largest quantities in the lower parts of the intestine, where the contents have the most disagreeable odor. No putrefaction occurs, on the contrary, in the intestinal canal of the foetus, which follows from the fact, proved by ZWEIFEL, HOPPE-SEYLER, and SENATOR, that in the contents of the same only undecomposed bile-acids and bile-pigments occur, while the otherwise regularly-occurring products of putrefaction in the intestinal canal are absent. The putrefaction processes in the intestine are somewhat differ- ent from those of the pancreas digestion ; and these two processes are essentially different from each other in the products which they yield. In the pancreatic digestion there are formed, so far as is known, besides albumoses and peptones, amido-acids and ammonia. 216 PHYSIOLOGICAL CHEMISTRY. In the putrefaction of the proteids we have, indeed, the same pro- ducts formed at the beginning, but the decomposition proceeds considerably further and a number of products are developed, which have become known through the labors of numerous inves- tigators, NENCKI, BAUMANN, BRIEGEE, H. and E. SALKOWSKI. The products which are formed in the putrefaction of proteids are (in addition to albumoses, peptones, amido-acids, and ammonia) indol, skatol, paracresol, phenol, plienyl-propionic acid, &n&'phenyl- acetic acid, also paraoxyphenyl-acetic acid and hydroparacoumaric acid (besides paracresol, produced in the putrefaction of tyrosin), volatile fatty acids, carbon dioxide, hydrogen, marsh-gas, and sul- phuretted hydrogen. In the putrefaction of gelatin neither tyrosin nor indol is formed, while glycocoll is produced. Among these products of decomposition a few are of special interest because of their behavior within the organism, and because after their absorption they pass into the urine. A few, such as the oxyacids, pass unchanged into the urine, while others, such as phenol, are transformed into ethereal sulphuric acids by synthesis, and are eliminated by the urine ; others, on the contrary, such as indol and skatol, are only converted into ethereal sulphuric acids after oxidation (for details see Chapter XIV). The quantity of these bodies in the urine varies also with the extent of the putre- faction processes in the intestine ; at least this is true for the ethe- real sulphuric acids. Their quantity increases with a stronger pu- trefaction, and the reverse takes place, as BAUMAKN" has shown by experiments on dogs, when the intestine has been disinfected by calomel, as then they disappear from the urine. Among the products of putrefaction developed in the intestine, indol and skatol must be carefully discussed. CH / % Indol, C8H7N = C6H4 CH, and Skatol, or METHYL- C.C.Hs C9H9N = C6H^ CH, are two bodies which stand DIGESTION. 217 in close relationship to the indigo substances, and which are formed from the albuminous bodies by their putrefaction, or by fusion with caustic alkali. Hence they occur habitually in the human intestinal canal and after oxidation into indoxyl and skatoxyl respectively, pass, at least partly, into the urine as the corresponding ethereal sulphuric acids, but also as glycuronic acids. These two bodies have been prepared synthetically in many ways. Both may be obtained from indigo by reducing it with tin and hydrochloric acid and heating this reduction product with zinc-dust (BAEYER). Indol may be formed from skatol by passing it through a red-hot tube. Indol suspended in water is in part oxidized into indigo-blue by ozone (NENCKI). Indol and skatol crystallize in shining leaves, and their melting points are -j- 52° and 95° respectively. Indol has a peculiar excre- mentitious odor, while skatol has an intense fetid odor (skatol ob- tained from indigo should be odorless). Both bodies are easily volatilized by steam, skatol more easily than indol. They may both be removed from the watery distillate by ether. Skatol is the more insoluble of the two in boiling water. Both are easily soluble in alcohol, and give with picric acid a combination consisting of red crystalline needles. If a mixture of the two picrates be distilled with ammonia, they both pass over without decomposition ; but if they are distilled with caustic soda, the indol is decomposed but not the skatol. The watery solution of indol gives with fuming nitric acid a red liquid, and then a red precipitate of nitroso-indol nitrate (BAEYER). It is better to first add two or three drops of nitric acid, and then a 2$ solution of potassium nitrite, drop by drop (SALKOWSKI). Skatol does not give this reaction. An alcoholic solution of indol treated with hydrochloric acid colors a pine chip cherry-red. Skatol does not give this reaction. Skatol dissolves in concentrated hydrochloric acid with a violet coloration. For the detection of indol and skatol in, and their preparation from, excrement and putrefying masses, the main points of the usual method are as follows : The mass is distilled after acidifying with acetic acid; the distillate is then treated with alkali (to com- bine with any phenol which may be present at the same time) and again distilled. From this second distillate the two bodies, after 218 PHYSIOLOGICAL CHEMIS1RY. the addition of hydrolichloric acid, are precipitated by picric acid. The picrate precipitate is then distilled with ammonia. The two bodies are obtained from the distillate by repeated shaking with ether and evaporation of the several ethereal extracts. The residue, containing indol and skatol, is dissolved in a very small quantity of absolute alcohol and treated with 8-10 vols. of water. Skatol is precipitated, but not the indol. The further treatment necessary for their separation and purification will be found in other works. The gases which are produced by the putrefactive processes are mixed in the intestinal tract with the atmospheric air swallowed with the saliva, and as the gas generated by different foods vary, so the mixture of gases after various foods should have a dissimilar composition. This is found to be true. Oxygen is only found in very faint traces in the intestine; this may be accounted for in part by the formation of reducing substances in the fermentation processes which combine with the oxygen, and partly, perhaps chiefly, to a diffusion of the oxygen through the tissues of the walls of the intestine. To show that these processes take place mainly in the stomach the reader is referred to page 189, on the composi- tion of the gases of the stomach. Nitrogen is habitually found in the intestine, and it is probably due chiefly to the swallowed air, or perhaps in part, as BUNGE claims, to a diffusion of nitrogen from the tissues of the intestinal walls to the intestine. The * carbon dioxide originates partly from the putrefaction of the pro- teids, partly from the lactic-acid and butyric-acid fermentation of carbohydrates, and partly from the setting free of carbon dioxide from the alkali carbonates of the pancreatic and intestinal juices by their neutralization by the hydrochloric acid of the gastric juice and by organic acids formed in the fermentation. Hydrogen occurs in largest quantities after a milk diet and in smallest quantities after a purely meat diet. This gas seems to be formed chiefly from the butyric-acid fermentation of carbohydrates, although it may occur in large quantities in the putrefaction of proteids under cer- tain circumstances. There is no doubt that the sulphuretted hydrogen which occurs normally in the intestine originates from the proteids. The marsh-gas undoubtedly originates in the putre- faction of proteids. As proof of this RUGE found 26.45$ marsh- gas in the human intestine after a meat diet. He found a still o DIGESTION. 219 greater quantity of this gas after a diet consisting of leguminous plants; this coinsides with the observation that marsh-gas may be produced by a fermentation of carbohydrates, but especially of cel- lulose (HOPPE-SEYLER, TAPPENIER). Such an origin of marsh- gas, especially in herbivora, is to be expected. A small part of the marsh-gas and carbon dioxide may also depend on the decomposition of lecithin (HASEBROEK). Putrefaction in the intestine not only depends upon the com- position of the food, but also upon the albuminous secretions and the bile. Among the constituents of bile which are changed or decomposed, we have not only the pigments — produced from the bilirubin, as is generally assumed, hydrobilirubin and brown pig- ments— but also the bile-acids, especially taurocholic acid. Glyco- cholic acid is more stable and a part is found unchanged in the ex- crement of certain animals, while taurocholic acid is so completely decomposed that it is entirely absent in the faeces. In the foetus, in whose intestinal tract no putrefaction processes occur, we find, on the contrary, undecomposed bile-acids and bile-pigments in the contents of the intestine. That the secretion rich in albumin is an important element in putrefaction in the intestine follows from the fact that putrefaction may also continue during fasting. From the observations of MULLER on CETTI it was found that the elimi- nation of indican during hunger rapidly decreased and after the third day of starvation it had entirely disappeared, while the phenol elimination, which at first decreased so that it was nearly minimum, increased again from the fifth day of starvation and on the eighth or ninth day it was three to seven times as much as in man under ordinary circumstances. In dogs, on the contrary, the elimi- nation of indican during starvation is considerable, but the phenol elimination is minimum. Among the secretions which undergo putrefaction in the intestine, the pancreatic juice, which putrifies most readily, takes first place. PISENTI found, in his experiments on dogs, that the elimination of indican by the urine greatly diminished after tying the pancreatic passages, but that it increased again when the animal was given pancreas peptones or pancreatic juice. From the foregoing facts we conclude that the products formed by the putrefaction in the intestine are in part the same as those 220 PHYSIOLOGICAL CHEMISTRY. formed by digestion. The putrefaction may be of benefit to the organism so far as the formation of such products as albumoses, peptones, and perhaps also certain amido-acids is concerned. On the contrary, the formation of further splitting products is to be considered as a loss of valuable material, and it is therefore impor- tant that putrefaction in the intestine is kept within certain limits. If an animal is killed while digestion in the intestine is going on, the contents of the small intestine give out a peculiar but not putrescent odor. Also the odor from the contents of the large intestine is far less offensive than a putrefying pancreas in- fusion or a putrefying mixture rich in albumin. From this we may conclude that putrefaction in the intestine is ordinarily not nearly so intense as outside of the organism. It seems thus to be provided, under physiological conditions, that putrefaction shall not proceed too far, and the factors which here come under consideration are probably of different kinds. Absorption is one of the more important of them, and it has been proved by actual observation that the putrefaction increases, as a rule, as the absorption is checked and fluid masses accumulate in the intestine. The character of the food also has an unmistakable influence, and it seems as if a large quantity of carbohydrates in the food acts against putrefaction (HIRSCHLER). A specially strong action tending to prevent putrefaction is observed in the bile. This anti-putrid action does not occur in neutral or faintly -alkaline bile, which itself easily putrefies, but in the free bile-acids, especially in taurocholic acid (MALY and EMICH, LINDBERG^ER). There is no question that the free bile-acids have a strong preventive action on putrefaction outside of the organism, and it is therefore difficult to deny such an action in the intestine. Notwithstanding this the anti -putrid action of the bile in the intestine is contradicted by certain investigators (Vorr, ROHMANN). Biliary fistulas have been introduced so as to study the impor- tance of the bile in digestion (ScHWANisr, BLONDLOT, BIDDER and SCHMIDT, and others). As a result it has been observed that from fatty foods an imperfect absorption of fat regularly takes place, and the excrements contain, therefore, an excess of fat and have a light-gray or pale color. How long after the operation the devia- tion from the normal appears depends essentially upon the char- , DIGESTION. 221 acter of the food. If an animal is fed on meat and fat, then the quantity of food must be considerably increased after the operation, otherwise the animal will become very thin, and indeed die with symptoms of starvation. In these cases the excrements have the odor of carrion, and this was considered a proof of the action of the bile in checking putrefaction. The emaciation and the increased want of food depend, naturally, upon the imperfect absorption of the fats, whose high calorific value is reduced and must be replaced by the taking up of larger quantities of other nutritive bodies. If the quantity of proteids and fats be increased, then this last, which can be only very incompletely absorbed, accumulates in the intes- tine. This accumulation of the fats in the intestine only renders the action of the digestive juices on proteids more difficult, and these last increase the amount of putrefaction. This explains the appearance of stinking faeces, whose pale color is not due to a lack of bile-pigments, but to a surplus of fat (ROHMANN, VOIT). If the animal is, on the contrary, fed on meat and carbohydrates, it may remain quite normal, and the leading off of the bile does not cause any increased putrefaction. The carbohydrates may be absorbed unprevented in such large quantities that they replace the fat of the food, and this is the reason why the animal on such a diet does not become emaciated. If with this diet the putrefaction in the intestine is no greater than under normal conditions even though the bile is absent, it would seem that the bile in the intes- tine exercise no preventive action on putrefaction. We must remember, however, that the presence of free acids counteracts putrefaction, and further that the carbohydrates yield free acids by acid fermentation within the intestine. It is there- fore conceivable that to the carbohydrates, which, according to HIRSCHLER, are capable of checking putrefaction without entering into an acid fermentation, the antiseptic action of the bile is due. It cannot be denied that the bile under ordinary conditions, with a mixed diet deficient in carbohydrates, has a preventive action on the putrefaction in the intestine. LIMBOURG has shown that it acts in an antiseptic sense, so that the decay of the proteids, giv- ing rise to simpler products less valuable, or perhaps even injurious, in the organism, are checked. Although the question how the putrefactive processes in the 222 PHYSIOLOGICAL CHEMISTKY. intestine under physiological conditions are kept within certain limits cannot be answered positively, still it may be asserted that the acid reaction of the upper parts of the intestine and the absorption of water in the lower parts are important factors. Excrements. It is evident that the residue which remains after completed digestion and absorption in the intestine must be differ- ent, both qualitatively and quantitatively, according to the variety and quantity of the food. In man the quantity of excrement from a mixed diet is 120-150 grms., with 30-37 grms. solids, per 24 hours, while the quantity from a vegetable diet, according to VOIT, was 333 grms., with 75 grms. solids. With a strictly meat diet the excrements are scanty, pitch-like, and colored nearly black by haematin and iron sulphide. The scanty excrements in starvation have a similar appearance. A large quantity of coarse bread yields a great amount of light-colored excrement. If there is a large proportion of fat, it takes a lighter, clayey appearance. The decom- position products of the bile-pigments seem to play only a small part in the normal color of the faeces. The constituents of the faeces are of different kinds. We find in the excrements digestible or absorbable constituents of the food, such as muscular fibres, connective tissues, lumps of casein, grains of starch, and fat which have not had sufficient time to be com- pletely digested or absorbed in the intestinal tract. In addition the excrements contain indigestible bodies, such as remains of plants, keratin substances, nuclein, and others; also form-elements originating from the mucous coat and the glands ; constituents of the different secretions, such as mucin, cholalic acid, dyslysin, and cholesterin; mineral bodies of the food and the secretions; and lastly, products of putrefaction or of the digestion, such as skatol, indol, volatile fatty acids, lime, and magnesia soaps. Occasionally, also, parasites of different kinds occur ; and lastly, the excrements contain micro-organisms, fungi of different kinds, sometimes in such large quantities that the chief mass of the excrements seems to consist of micro-organisms (v. JAKSCH). The reaction of the excrements is very changeable. It is often #cid in the inner part, while the outer layers in contact with the mucous coat have an alkaline reaction. In nursing infants it is DIGESTION. 223 habitually acid (WEGSCHEIDER). The odor is perhaps chiefly due to skatol, which was first found in the excrements by BRIEGER, and so named by him. Indol and other substances also take part in the production of odor. The color is ordinarily lighter or darker brown, and depends above all upon the nature of the food. Medicinal bodies may give the faeces an abnormal color. The ex- crements are colored black by iron and bismuth, yellow by rhubarb, and green by calomel. This last-mentioned color was formerly accounted for by the formation of a little mercury sulphide, but now it is said that calomel checks the putrefaction and the decom- position of the bile-pigments, so that a part of the bile-pigments pass into the faeces as biliverdin. According to LESAGE, a green color of the excrements in children is caused partly by biliverdin and partly by a pigment produced from a bacillus. In the yolk-yellow or greenish-yellow excrements of nursing infants we can detect bilirubin. Neither bilirubin nor biliverdin seems to exist in the excrements of mature persons under normal conditions. On the contrary, we find STERCOBILIN' (MASIUS and VANLAIR), which, ac- cording to certain investigators, is identical with hydrobilirubin (MALY), which is obtained from bilirubin by a reduction process, and urobilin (JAFFE) — a view contested by MAcMuNN. Bilirubin may occur in pathological cases in the faeces of mature persons. It has been observed in a crystallized state (as haematoidin) in the faeces of children as well as of grown persons (UFFELMANN, v. JAKSCH). The absence of bile (acholic faeces) causes the excrements to have, as above stated, a gray color, due to large quantities of fat; this may, however, be partly attributed to the absence of bile-pig- ments. In these cases a large quantity of crystals has been ob- served (GERHARDT, v. JAKSCH) which consist chiefly of magnesia soaps (OESTERLEN) or sodium soaps (STADELMANN). Hemorrhage in the upper parts of the digestive tract yields, when it is not very abundant, a dark-brown excrement, due to haematin. EXCRETIN, so named by MARCET, is a crystalline bodv occurring in human excrement, but which, according to HOPPE-SEYLER, is perhaps only impure cholesterin. EXCRETOLIC ACID is the name given by MARCET to a body similar to oil and with an excrementiteous odor. In consideration of the very variable composition of excrements their quantitative analyses are of little value and therefore will be omitted. 224 PHYSIOLOGICAL CHEMISTRY. Meconium is a dark brownish-green, pitchy, mostly acid mass without any strong odor. It contains greenish-colored epithelium cells, cell-detritus, numerous fat-globules, and cholesterin plates. The amount of water and solids is respectively 720-800 and 280- 200 p. m. Among the solids we find mucin, bile-pigments and bile-acids, cholesterin, fats, soaps, calcium and magnesium phos- phates. Sugar and lactic acid, albuminous bodies (?) and peptones, also leucin and tyrosin and the other products of putrefaction occurring in the intestine are absent. Meconium may contain undecomposed taurocholic acid, bilirubin and biliverdin, but it does not contain any hydrobilirubin, which is considered as proof of the non-existence of putrefactive processes in the digestive tract of the foetus. In medico-legal cases it is sometimes necessary to decide whether spots on linen or other substances are caused by meconium. In such cases we have the following conditions. The spot caused by meconium has a brownish-green color and can be easily separated from the material because, on account of the ropy property of the meconium, it is difficult to wet through. When moistened with water it does not develop any special odor, but on warming with dilute sulphuric acid it has a somewhat fetid odor. It forms with water a slimy, greenish-yellow liquid containing brown flakes. The solution gives with an excess of acetic acid an insoluble precipitate of mucin ; on boiling it does not coagulate. The filtered, watery extract gives GMELIN'S, but still better HUPPERT'S, reaction for bile-pigments. The liquid precipitated by an excess of milk of lime gives a nearly colorless filtrate, which after concentration gives PETTENKOFER'S reaction. The contents of the intestine under abnormal conditions are per- haps less the subject of chemical analysis than of an inspection or microscopical investigation. On this account the question as to the properties of the contents of the intestine in different diseases cannot be thoroughly treated here. The question as to the differ- ent processes which, so far as they are dependent on secretion and absorption, cause an abnormal consistency, a thinning of the excre- ments, possesses a certain interest. Such excrements may in part be produced by arrested absorption of liquid from the intestine for some reason or other, and in part caused by an increased secre- tion or a transudation of liquids into the intestine. DIGESTION. A diminished absorption (of water) may be caused by a more active movement of the intestine, which causes their contents to pass quickly, and in this way the action of laxatives is often ex- plained. A diminished absorption may also be due to a decreased activity of the absorbing cells. In absorption, which is generally accepted to-day, the cells of the mucous coat take an active part, and anything which acts disturbingly on the protoplasm of these cells must also exercise an influence on the absorption. This con- dition with regard to the action of laxatives has been especially noted by HOPPE-SEYLER. According to him, it is also probable that such laxatives, of which only traces are required for absorption, by a direct action on the intestinal epithelium — whether the absorp- tion is made more difficult, or a transudation made possible, or whether the action of these two is simultaneous — cause watery evacuations. According to ROHMANK, concentrated salt solutions act by a decreased absorption activity. A thin evacuation may be produced by an increased elimination of fluid into the intestine, and there are many investigators who consider it positively proved that a transudation of liquid into the intestine is caused by the action of saline laxatives. The character of the intestinal epithelium is undoubtedly an important factor in the production of such a transudation, and when this is caused by the saline laxatives it probably is produced by action on the epithelium. We must admit with HOPPE-SEYLER and other investigators that the most important regulator of the flow of liquid through the intestinal mucous membrane is the intestinal epithelium. It is the epithelium which renders possible the stream of fluid contrary to the laws of osmose, and which under normal conditions prevents a trausudation into the intes- tines. Bodies which affect the epithelium may therefore cause a transudation, and this is found to be especially abundant after ejection of the intestinal epithelium. The most striking example of this is observed in Asiatic cholera, in which the epithelium is largely expulsed and an extraordinarily abundant transudation takes place. 226 PHYSIOLOGICAL CHEMISTRY. Appendix. Intestinal Conor ements. Calculi occur very seldom in human intestine or in the intestine of carnivora, but they are quite common in herbivora. Foreign bodies or indigested residues of food may, when for some reason or other they are retained in the intestine for some time, become incrusted with salts, especially ammonium-magnesium phosphate or magnesium phosphate, and these salts form usually the chief constituent of the concrements. In man they are sometimes oval or round, yellow, yellowish-gray, or brownish-gray, of variable size, consisting of concentric layers and containing chiefly ammonium- magnesium phosphate, calcium phosphate, besides a small quantity of fat or pigment. The nucleus ordinarily consists of some foreign body, such as the stone of a fruit, a fragment of bone, or something similar. In those countries where bread made from oat-bran is an important food, we often find in the large intestine balls similar to the so-called hair-balls (see below). Such calculi contain calcium and magnesium phosphate (about 70$), oat-bran (15-18$), soaps and fat (about 10$). Concretions which contain very much (about 74$) fat occur seldom, and those consisting of fibrin clots, sinews, or pieces of meat incrusted with phosphates are also rare. Intestinal calculi occur often in animals, especially in horses fed on bran. These calculi, which attain a very large size, are hard and heavy (as much as 8 kilos) and consist in great part of concen- tric layers of ammonium-magnesium phosphate. Another variety of concrements which occurs in horses and cattle consists of gray colored, often very large, but relatively light stones which contain plant-remains and earthy phosphates. Stones of a third variety are sometimes cylindrical, sometimes spherical, smooth, shining, brownish on the surface, consisting of matted hairs and plant- fibres, and termed hair-balls. The so-called " .EGAGROPILA," which probably originate from the ANTILOPUS RUPICAPEA, belong to this group, and they are generally considered as nothing else than the hair-balls of cattle. The so-called oriental bezoar-stone belongs also to the intestinal concrements, and probably originates from the CAPEA ^GAGRUS and ANTILOPE DORCAS. We may have two varieties of bezoar- DIGESTION. 227 etones. One is olive-green, faintly shining, formed of concentric layers. On heating it melts with the development of an aromatic odor. It contains as chief constituent LITHOFELLIC acid, C^H^Ot , which is related to cholalb acids, and besides this a bile-acid, LITHOBILIC ACID. The others are nearly blackish brown or dark green, very glossy, consisting of concentric layers, and do not melt on heating. They contain as chief constituent ELLAGIC ACID, a de- rivative of tannic acid, of the formula CUH608, which gives a deep blue color with an alcoholic solution of ferric chloride. This last- mentioned bezoar-stone originates, to all appearances, from the food of the animal. Ambergris is generally considered an intestinal concrement of the sperm- whale. Its chief constituent is AMBBAIN, which is a non-nitrogenious sub- stance perhaps related to cholesterin. Ambrain is insoluble in Vater and is not changed by boiling alkalies. It dissolves in alcohol, ether, and oils. VI. Absorption. In discussing the absorption processes we must treat of the form into which the different foods are transformed before absorp- tion, of the manner in which this is accomplished, and, lastly, of the force which acts in these processes. Starch and the other carbohydrates are chiefly absorbed as sugar, in part also as organic acids (lactic acid), and perhaps in small quantities as dextrin. Fats may indeed be partly absorbed as soaps; still the quantity absorbed in this way is very small compared with that absorbed as an emulsion. Emulsion is undoubtedly the most important form under which fat is absorbed, and the neutral fats, also the free fatty acids, are emulsified when they occur in large quantities in the intestines. Peptone, as above stated, is the final product of the digestion of albuminous bodies. Now as peptone is a very soluble and a rela- tively easily diffusible modification of proteids, it is not difficult to admit the deduction that proteids must be changed into peptone in order that it may be readily absorbed. Certain observations of FUNKES on animals confirm this view. He found in an untied intestinal knot of a living animal that the peptone (in the old sense of the word) was absorbed considerably faster than other proteids. There is also no doubt that a part of the proteids is 228 PHYSIOLOGICAL CHEMISTRY. invariably absorbed from the intestinal canal as peptones, or more correctly perhaps as albumoses and peptones. But it has been positively settled by the investigations of BRUCKE, BAUER and VOIT, EICHHORST, CzERNY and LATSCHENBERGER, that peptonized albumin, casein, myosin, and alkali albuminates cannot be ab- sored from the intestines — a matter which is of practical import- ance, especially with regard to the nutritive clysters. If the albumin can be absorbed partly as such and partly as peptone, then the question arises, how much more can it be absorbed in one form than in the other ? This question cannot be decisively answered. The investigations by SCHMIDT-MULHEIM of the contents of the stomach and intestine of dogs show that the stomach contains a considerably larger amount of peptone than of simple dissolved proteids, which seems to indicate that the greatest part of the pro- teids is absorbed as peptones (or albumoses). The soluble salts are also absorbed with the water. The albu- min and peptone which can dissolve a considerable quantity of salts insoluble in alkaline water are of great importance in the ab- sorption of such salts. The soluble constituents of the digestive secretions may, like other dissolved bodies, be absorbed, as is demonstrated by the pas- sage of peptone into urine ; the enzymes may also be absorbed. The occurrence of urobilin in urine attests to the absorption of the bile-constituents under physiological conditions notwithstanding that, according to certain investigators (MAcMu^N), it is not identical with hydrobilirubin, and contrariwise, according to the observations of COPEMAN and WINSTON on a woman with a biliary fistula, it also occurs in the urine when no bile comes into the intestine. The question as to the occurrence of very faint traces of bile-acids in normal urine is also contradicted, and from the behavior of the urine it is therefore difficult to draw any positive conclusion as to a possible absorption of the bile-constituents from the intestine. On the other hand, the absorption of bile-acids from the intestine seems to be established by other observations. TAPPEINER introduced a solution of bile-acid salts of a known concentration into an untied intestinal knot, and after a time investigated the contents. He found that in the jejunum and the ileum, but not in the duodenum, an absorption of bile-acids took DIGESTION. 229 place, and further that of the two bile-acids only the glycocholic acid was absorbed in the jejunum. Further, SCHIFF long ago expressed the opinion that bile undergoes an intermediate circula- tion, in such wise that it is absorbed from the intestine, then carried to the liver by the blood, and lastly eliminated from the blood by this organ. Although this view has met with some oppo- sition, still its correctness seems to be established by the researches of various investigators, and more recently by PREVOST and BINET, at least to the extent that after the introduction of foreign bile into the intestine of an animal the foreign bile-acids appear again in the secreted bile. There are two possible ways in which the absorbed bodies may enter the blood-stream. They may pass into the blood through the chylous vessels and the thoracic duct indirectly, or they may, after they have passed the intestinal epithelium, pass into the blood-capillaries of the villous membranes and so directly into the blood. By the investigations of LUDWIG and his pupils, ROHRIG, ZAWILSKY, v. MERING and SCHMIDT-MULHEIM, as well as by those of HEIDENHAIN and* his pupils, it has now been proved that the fat is driven through the chylous vessels and the thoracic duct to the blood, while on the contrary the bodies soluble in water, such as sugar and salts, are taken up by the blood of the capillaries of the villous membrane and in this way pass into the blood. The reason why the dissolved bodies do not pass into the chylous vessels in larger quantities is explained by HEIDENHAIN to lie in the anatomi- cal situation, — the arrangement of the capillaries close under the layer of epithelium. Ordinarily these capillaries find the necessary time for the taking up of the water and the solids dissolved in it. But when a large quantity of liquid, such as a stagar solution, is introduced into the intestine at once, this is not possible, and in these cases a part of the dissolved bodies pass into the chylous vessels and the thoracic duct (GINSBERG and ROHMANN). The question of the absorption of the peptones, albeit in most cases these have not been differentiated from albumoses, has been the subject of numerous investigations. LUDWIG and SCHMIDT- MULHEIM tied the jugular and humeral arteries and lymphatic vessels of both sides of a dog so that the section showed later a complete cutting off of the chyle from the blood-circulation. They 230 PHYSIOLOGICAL CHEMISTRY. found that the absorption of proteids from the intestine was not influenced thereby, and from this concluded that the proteids as well as the other nutritive bodies soluble in water pass directly into the blood through the walls of the intestinal capillaries. If this were the case, then we might expect to find peptone in solution in the blood during or after digestion. This, however, is not the case. ScHMiDT-MtJLHEiM and HOFMEISTER found only traces of peptone in serum or blood, and according to NEUMEISTER it does not occur even as traces. Chyle does not contain any peptone. Then where does the peptone which is absorbed from the intestine remain ? If peptone in solution is introduced into the circulating blood, it is quickly eliminated therefrom with the urine (PLOS'Z and GYERGYAI, HOFMEISTER, SCHMIDT-MULHEIM). The same happens also after injecting peptone subcutaneously. Nor- mal urine does not contain any peptone, and the absence of this body in the blood after digestion cannot be explained by assuming that it is eliminated by the kidneys, Since the peptone introduced into the blood is quickly eliminated by the kidneys, while the peptone formed in the intestine does not pass into the urine, it may perhaps be thought that this peptone is retained normally by the 'liver and is absorbed, and only that peptone which finds its way into the circulating blood by evasion from this organ passes into the urine. This supposition, however, is untenable. NEU- MEISTER has investigated the portal blood of rabbits in whose stomachs large quantities of albumoses and peptones had been introduced, and found therein only traces of the body in question. He has also shown that when we supply the liver of a dog with the portal-blood peptone (ampho-peptone), this is not retained by the liver but is eliminated with the urine. Peptone seems to pass neither into the blood nor the chylous vessels, but to be changed in some way by the walls of the intestine. HOFMEISTER, according to whom the walls of the stomach and the intestine are the only parts of the body in which peptones occur constantly during diges- tion, has also made the observation that peptone (at the tempera- ture of the body) after a time disappeared from the excised but apparently still living mucous coat of the stomach. Peptone seems to undergo a change in the mucous membrane of the digestive canal, and the following observation of LUDWIG and SALVIOLI DIGESTION. 231 bears out that assumption. These investigators introduced a peptone solution into a doubly-tied, removed knot of the small intestine which was kept active by passing through defibrinated blood, and observed that the peptone disappeared from the intes- tine, but that the blood passing through did not contain any pep- tone. If, then, peptone already disappears in the mucous coat, or at least in the walls of the digestive tract, the question naturally arises, what becomes of the peptone in the mucous membrane ? The experiments of MALY, PLOS'Z and GYERGYAI, ADAMKIEWICZ, ZUKTZ and POLLITZER have established that the . albumoses and peptones may be substituted for albumin in the food, and may also be converted into ordinary albumin. We must then assume that peptone is already converted into albumin in the mucous mem- brane of the digestive canal. According to HOFMEISTER, a considerable increase of leucocytes occurs in the adenoid tissues during digestion, an observation which is in close accord with that of POHL, who found that in dogs kept on an albuminous diet the venous blood of the intestine contains more leucocytes than the arterial blood. According to HOFMEISTER, leucocytes play an important part in the absorption and assimilation of the peptones. They may take up the peptones and be the means of transporting them to the blood, and secondly by their growth, regeneration, and increase may stand in close rela- tion to the transformation and assimilation of the peptones. HEIDENHAIN, who considers that the transformation of peptone into albumin in the mucous membrane is positively settled, does not, attribute so great an importance to these last in the absorption of the peptones as HOFMEISTER, chiefly on the ground of equaliza- tion of the quantity of absorbed peptones and leucocytes. He con- siders it most probable that the reconversion of the peptones into albumin takes place in the epithelium layers. Little is known concerning the forces taking part in absorption. Osmose and filtration were formerly considered as the most impor- tant factors. • But as in regard to the peptones, whose formation in the digestion was considered as taking place especially in the interest of a facilitated osmosis and filtration, but whose conditions have been found quite different and much more complicated, so in 232 PHYSIOLOGICAL CHEMISTRY. the absorption theory there is a still greater contrast between former and present views, the latter inclining to the theory that absorption is a process connected with the vital properties of the cells. Investigations in this direction have been made by HEIDEN- HAIK and his pupils, KOHMANN and GUMILEWSKY; and these investigations have shown that the cells take an active part in the absorption, and that this action is independent of the processes caused by an unequal diffusibility of the different bodies. For example, in a solution which contains equal quantities of grape- sugar and sodium sulphate the sugar will be almost completely absorbed in a certain time, while the salt, which has the greater diffusibility, still remains in considerable amounts in the intestine. Certain coloring matters are not absorbed, and the cells seem to have the property of discriminating between the different sub- stances. The absorption of dissolved bodies seems to be connected with a specific activity of the living cell, the living protoplasm. In the absorption of bodies not dissolved, of the emulsified fats forces take part which are not known. That the bile performs the most important part in the absorption of fats is very generally admitted, but how the bile acts in this process is not yet deter- mined, v. WISTINGHAUSEN has found that fat rises higher in a capillary tube when it has been moistened with bile than when with water, and further that fluid fat filters more easily through a dead membrane dipped in bile than when dipped in water. From these observations, whose correctness has lately been disputed by GAD and GROPER, the inference has been drawn that bile facilitates the capillary attraction and thereby accelerates the absorption of the fats. The epithelium layer of the intestinal mucous membrane cannot be compared with a dead membrane soaked in water, and it is therefore doubtful if the above-mentioned action of bile can have any influence on the absorption of fats in the intestine. That the absorption of fats is caused by the lymphoidal migratory cells (ZAWARYKIN, SCH^FFER) is disputed by GRUENHAGEK and HEIDENHAIN. According to them, the fat takes its way chiefly through the epithelium cells. How these last act is, like the nature of the action in absorption, enveloped in darkness. CHAPTER VIII. TISSUES OF THE CONNECTIVE SUBSTANCE. I. The Connective Tissues. THE form-elements of the typical connective tissues are cells of various kinds, of a not very well known chemical composition, and gelatine-yielding fibrils. Besides these, elastic formations are often found in variable amounts, though sometimes in such predominant quantities that the connective tissue passes nearly into an elastic tissue. Mucin is also found in the connective tissue, serum globulin and serum albumin occurring in the parenchymatous fluid (LOEBISCH). If finely-divided tendons are extracted in cold water, the albu- minous bodies soluble in the nutritive fluid in addition to a little mncin are dissolved. If the residue is extracted with half-satu- rated lime-water, then the mucin is dissolved (ROLLETT, LOEBISCH) and may be precipitated from the filtered extract by saturating with acetic acid. The digested residue contains the fibrils of the connective tissue together with the cells and the elastic substance. The fibrils of the connective-tissue consist of collagen. They are elastic, swell slightly in water, somewhat more in diluted alkalies or acetic acid, but on the other hand are shrivelled by the action of certain metallic salts, such as ferrous sulphate or mercuric chloride, and by the action of tannic acid, which forms with collagen an insoluble combination. Among these combina- tions, which prevent the putrefaction of collagen, that with tannic acid has been found of great value in the preparation of leather. In regard to tendon mucin see page 32, and in regard to collagen, glutin, and elastin see pages 36-38. 234 PHYSIOLOGICAL CHEMISTRY. The tissues described under the names mucous or gelatinous tissues are characterized more by their physical than their chemical properties and have been but little studied. So much, however, is known, that the mucous or gelatinous tissues contain, at least in certain cases, as in the acalephae, no mucin. The nmbilical cord is the most accessible material for the inves- tigation of the chemical constituents of the gelatinous tissues. The mucin occurring therein has been described on page 32. C. TH. MoRtfER has found a mucoid in the vitreous humor which contains 12.20$ nitrogen and 1.19$ sulphur. II. Cartilage. Cartilaginous tissue consists of cells and of a basic substance originally hyaline, which, however, may become changed in such wise that there appears in it a network of elastic fibres or connec- tive-tissue fibrils. Those cells that offer great resistance to the action of alkalies and acids have not been carefully studied. According to former views, the basic substance was considered as consisting of a body analogous to collagen, the so-called cJiondrigen, which under similar conditions passes, like collagen, into a corresponding gelatine called chondrin or cartilage-gelatine. The more recent investigations of MOROCHOWETZ and others, but especially those of C. TH. MORNER, have shown that the basic substance of the cartilage consists of a mixture of collagen with other bodies. The tracheal, th^roideal, cricoidal, and arytenoidal cartilages of full-grown cattle contain, according to MORNER, four consti- tuents in the basic substance, namely, chondromucoid, cliondroitic acid, collagen, and albumoid. Chondromucoid. This body, according to MORNER, has the composition 047.30, H 6.42, N 12.58, S 2.42, 0 31.28 per cent. Sulphur is in part loosely combined and may be split off by the action of alkalies, and a part separates as sulphuric acid when boiled with hydrochloric acid. Chondromucoid is decomposed by dilute alkalies and yields alkali albuminate, peptone substance, chondroitic acid, alkali sulphides, and some alkali sulphates. On TISSUES OF THE CONNECTIVE SUBSTANCE. 235 boiling with acids it yields acid albuminate, peptone substance, chondroitic acid, and on account of the further decomposition of this last body sulphuric acid and a reducing substance are formed. Chondromucoid is a white, amorphous, acid-reacting powder which is insoluble in water, but dissolves easily on the addition of a little alkali. This solution is precipitated by acetic acid in great excess and by small quantities of mineral acids. The precipitation may be retarded by neutral salts or by chondroitic acid. The solution containing NaCl and acidified with HC1 is not precipitated by potassium ferrocyanide. Precipitants for chondromucoid are alum, ferric chloride, sugar of lead or basic lead acetate. Chon- dromucoid is not precipitated by tannic acid, and it may by its presence prevent the precipitation of gelatine by this acid. It gives the usual color reactions for albuminous bodies; namely, with nitric acid, with copper sulphate and alkali, with MILLON'S and ADAMKIEWICZ'S reagents. Chondroitic Acid. This acid, which thus far has not been ob- tained free, but as acid salts, has, according to MORNEB. the follow- ing composition : C 35.28, H 4.68, N 3.15, S 6.33, 0 50.56 per cent. On boiling with dilute hydrochloric acid all the sulphur splits off as H2S04 , and a reducing substance is formed at the same time whose nature is not known. This acid (more correctly the acid alkali salts) appears as a white amorphous powder which dissolves very easily in water, forming an acid solution and when sufficiently concentrated, a sticky liquid similar to a solution of gum arabic. The neutralized solution is precipitated by tin chloride, basic lead acetate, neutral ferric chloride, and by alcohol in the presence of a little neutral salt. The solution, on the other hand is not precipitated by acetic acid, tannic acid, potassium ferrocyanide and acid, sugar of lead, mercuric chloride, or silver nitrate. Chondroitic acid does not give the color reactions for albuminous bodies. Preparation of chondromucoid and chondroitic acid. If very finely-cut cartilage is extracted with water, the preformed chon- droitic acid, as well as some chondromucoid, is dissolved. In this watery extract the chondroitic acid prevents the precipitation of this chrodromucoid by means of an acid. If 2-4 p. m. 1 added to this watery extract and warmed on the water-bath, the 236 PHYSIOLOGICAL CHEMISTRY. chondromucoid gradually separates, while the chondroitic acid and the rest of the chondromucoid remain in the filtrate. If the car- tilage, which has been lixiviated, at the temperature of the body, with water, is extracted with hydrochloric acid of 2-3 p. m. until the collagen is converted into soluble gelatine, the remaining chon- dromucoid may be removed from the insoluble residue by dilute alkali and precipitated from the alkaline extract by an acid. It may be purified by repeatedly redissolving in water with the aid of a little alkali, precipitating by an acid and then treating with alcohol and ether. The chondroitic acid originally formed, or that formed by the decomposition of chondromucoid, is obtained by lixiviating the cartilage with a 5$ caustic-alkali solution. The alkali albuminate formed by the decomposition of the chondromucoid can be re- moved from the solution by neutralization, then the peptone precipitated by tannic acid, the excess of this acid removed with sugar of lead, and the lead separated from the filtrate by H?S. If further purification is necessary, the acid is precipitated with alcohol, the precipitate dissolved in water, this solution dialyzed and precipitated again with alcohol, this dissolving in water and precipitating with alcohol being repeated a few times, and lastly the acid is treated with alcohol and ether. The collagen of the cartilage gives, according to MORNER, a gelatine which contains only 16.4$ N and which can hardly be considered identical with ordinary glutin. On the above-mentioned cartilages of full-grown animals the chondroitic acid and chondromucoid, perhaps also the collagen, are found surrounding the cells as round balls or lumps. These balls (MORNER'S chondrin-balls), which give a blue color with methyl- violet, lie in the meshes of an albumoid structure, which is colored when brought in contact with tropaeolin. This albumoid is a nitrogenized body which contains loosely- combined sulphur. It is soluble with difficulty in acids and alka- lies, and resembles keratin in many respects, but differs from it bv being soluble in gastric juice. In other respects it is more similar to elastin, but differs from this substance by containing sulphur. This albumoid gives the color reactions of the albuminous bodies. The preparation of cartilage-gelatine and albumoid may be per- formed according to the following method of MORJ^ER. First re- move the chondromucoid and chondroitic acid by extraction with dilute caustic potash (0.2-0.5$), remove the alkali from the re- TISSUES OF THE CONNECTIVE SUBSTANCE. 237 muiuing cartilage by water, and then boil with water in a PAPIN'S digester. The collagen passes into solution as gelatine, while the albumoid remains undissolved (contaminated by the cartilage-cells). The gelatine may be purified by precipitating with sodium sul- phate, which must be added to saturation in the faintly-acidified solution, redissolving the precipitate in water, dialyzing well, and precipitating with alcohol. According to MORNER, no albumoid is found in young car- tilage, but only the three first-mentioned constituents. Neverthe- less the young cartilage contains about the same amounts of nitrogen and mineral substances as the old. HOPPE-SEYLER found in fresh rib cartilage 676.7 p. m. water, 301.3 p. m. organic and 22 p. m. inorganic substance. In the cartilage of the knee-joint 735.9 p. m. water, 248.7 p. m. organic and 15.4 p. m. inorganic substance have been found. The ash of cartilage contains considerable amounts (even 800 p. m. ) of alkali sulphate, which probably did not exist originally as such, but is produced in great part by the calcining of the chondroitic acid and the chondromucoid. The analyses of the ash of cartilage therefore cannot give a correct idea of the quantity of mineral bodies exist- ing in this substance. PETERSEN and SOXHLET have found 940 p. m. NaCl in the ash from the cartilage of a shark, and such cartilage can scarcely contain quantities of chondromucoid or chon- droitic acid worth mentioning. The cartilage of the ray (Raja batis LIN".), which has been investigated by LONNBERG, contains no albumoid and only a little chondromucoid, but a large propor- tion of chondroitic acid and collagen. The Cornea. The corneal tissue, which is considered by many investigators to be related to cartilage in a chemical sense, contains traces of albumin and a collagen as chief constituent, which C. TH. MORNER claims contains 16.94$ N. According to him it contains, a mucoid which has the composition C 50.16, H 6.98, N 12.8, and S 2.05 per cent. On boiling with dilute acid this mucoid yields a reducing substance. In the cornea of oxen His found 758.3 p. m. water, 203.8 p. m. gelatin forming substance, 28.4 p. m. other organic substance, besides 8.1 p. m. soluble and 1.1 p. m. insoluble salts. 238 PHYSIOLOGICAL CHEMISTRY. III. Bone. The bony structure proper, when free from other formations occurring in bones, such as marrow, nerves, and blood-vessels, con- sists of cells and a basic substance. The cells have not been closely studied in regard to their chemi- cal constitution. On boiling with water they yield no gelatine. They contain no keratin, which is not usually present in the bony structure (HERBERT SMITH), but they may contain a substance which is similar to elastin. The basic substance of the bony structure contains two chief constituents, namely, an organic substance, ossein, and the so-called bone-earths enclosed in or combined with it. If bones are treated with dilute hydrochloric acid at the ordinary temperature, the lime- salts are dissolved and the ossein remains as an elastic mass, pre- serving the shape of the bone. This ossein is generally considered identical with the collagen of the connective tissue. The ossein in the bones of certain aquatic fowls and fishes can hardly be consid- ered identical with this collagen (FREMY). The inorganic constituents of the bony structure, the so-called bone-earths, which remain after the complete calcination of the organic substance as a white, brittle mass, consist chiefly of calcium and phosphoric acid, but also include carbon dioxide and, in smaller amounts, magnesium, chlorine, and fluorine. Alkali sul- phate and iron, which are found in the bone-ash, do not seem to belong exactly to the bony substance, but to the nutritive fluids or to the other constituents of bones. The opinions of investigators differ somewhat as to the manner in which the mineral bodies of the bony structure are combined with each other. Chlorine and fluorine are present in the same form as in apatite (CaFl2 , 3Ca3P208). Proceeding from the chlorine and fluorine, it is possible that the other mineral bodies form the combination 3(Ca3P208)CaC03. Analyses of bone-earths have shown that the mineral constit- uents are related to each other in nearly constant proportions, and this applies not only to man but also to the different animals. As TISSUES OF THE CONNECTIVE SUBSTANCE. 239 x example of the constitution of bone-earth we give here the analyses of ZALEWSKY. The figures represent parts per thousand. Man. Ox. Tortoise. Guinea-pig. Calcium phosphate, Ca8P,O8 838.9 860.9 859.8 873.8 Magnesium phosphate, Mg3PaO8 10.4 10.2 18.6 10.5 Calcium combined with COa, Fl, and Cl. 76.5 73.6 63.2 70.3 Co, 57.3 62.0 52.7 Chlorine 1.8 2.0 1.8 Fluorine 2.8 8.0 2.0 Some of the CO, is always lost on calcining, so that the bone-ash does not contain the entire CO, of the bony substance. The quantity of organic substance in the bones, calculated from the loss of weight in burning, varies somewhat between 300 and 520 p. m. This variation may in part be explained by the difficulty in obtaining the bony substance entirely free from water, and partly by the very variable amount of blood-vessels, nerves, marrow, and the like in different bones. The unequal amounts of organic sub- stance found in the compact and in the spongy parts of the same bone, as well as in bones at different periods of development in the same animal, depend probably upon the varying quantities of these above-mentioned formations. Dentin, which is comparatively pure bony structure, contains only 260 to 280 p. m. organic sub- stance, and HOPPE-SEYLER therefore thinks it probable that en- tirely pure bony substance has a constant com posit ion and contains only about 250 p. m. organic substance. The question whether these substances are chemically combined with the bone-earths or only intimately mixed has not been decided. The nutritive fluids which circulate through the bones have not been iso- lated, and we only know that they contain some albumin and some NaCl and alkali sulphate. The yellow marrow contains chiefly fat. which consists of olein, palmitin, and stearin. Albumin has been found especially in the so- called red marrow of the spongy bones. Besides these substances, the marrow contains so-called extractive bodies, such as lactic acid, hypoxanthin, and cholesterin, but mostly bodies of an unknown character. The diverse quantitative composition of the various bones of the skeleton depends probably on the varying quantities of other formations, such as marrow, blood-vessels, etc., they contain. The same reason explains, to all appearances, the larger quantity of organic substance in the spongy parts of the bones as compared with the more compact parts. SCHRODT has made comparative analyses of different parts of the skeleton of the same animal (dog) 240 PHYSIOLOGICAL CHEMISTRY. and has found an essential difference. The quantity of water in the fresh bones varies between 138 and 443 p. m. The bones of the extremities and the skull contain 138-222, the vertebrae 168- 443, and the ribs 324-356 p. m. water. The quantity of fat varies between 13 and 269 p. m. The largest amount of fat, 256-269 p. m., is found in the long tubular bones, while only 13-175 p. m. fat is found in the small short bones. The quantity of organic substance, calculated from fresh bones, was 150-300 p. m., and the quantity of mineral substances 290-563 p. m. Contrary to the general supposition the greatest amount of bone-earths was not found in the femur, but in the three first cervical vertebrae. In geese the largest amount of bone-earth was found in the humerus (HlLLER). We do not possess trustworthy statements in regard to the com- position of bones at different ages. There is no question, however, that the mineral constituents increase until a certain age is reached, at which time the bones attain their necessary solidity. On the other hand, it is not certainly known whether this increase stops at a certain point or whether, as was formerly thought, it con- tinues, even if slowly, uninterrupted from childhood to old age. The composition of bones of animals of different species is but little known. The bones of birds contain, as a rule, somewhat more water than those of mammalia, and the bones of fishes contain the largest quantity of water. The bones of fishes and amphibians contain a greater amount of organic substance. The bones of pachydermata and cetaceae contain a large proportion of calcium carbonate ; those of granivorous birds always contain silicic acid. The bone-ash of amphibians and fishes contains sodium sulphate. The bones of fishes seem to contain more soluble salts than the bones of other animals. A great many tests have been made to determine the exchange of material in the bones — for instance, with food rich in lime and with food deficient in lime — but the results have always been doubtful or contradictory. The attempts, also, to substitute other alkaline earths or clay for the lime of the bones have given contra- dictory results. KEAPP found that the bones of the animals under experimentation were tinged with red after a few days or weeks ; but these tests have not led to any positive conclusion in regard to the growth or exchange of material in the bones. Under pathological conditions, as in rachitis and softening of the bones, an ossein has been found which does not give any WYIISITY TISSUES OF THE typical gelatine on boiling with water. Otherwise the pathological conditions seem to affect chiefly their quantitative composition, and especially the relationship between the organic and the inorganic substance. In exostosis and osteomalacosis the quantity of organic substance is generally increased. Attempts have been made to produce rachitis in animals by the use of food deficient in lime. From experiments on fully-developed animals contradictory results have been obtained. In young, undeveloped animals ERWIN VOIT produced, by lack of lime-salts, a change similar to rachitis. In full-grown animals the bones were changed after a long time because of the lack of lime-salts in the food, but did not become soft, only thinner, and atrophied. The experiment of removing the lime-salts from the bones by the addition of lactic acid to the food has led to no positive results (HEITZMANN", HEISS, BAGINSKY). A few investigators are of the opinion that in rachitis, as in osteomalacosis, a solution of the lime-salts by means of lactic acid takes place. This was suggested by the fact that 0. WEBER and C. SCHMIDT found lactic acid in the cyst-like, altered bony sub- stance in osteomalacia. Well-known investigators have disputed the possibility of the lime-salts being washed from the bones in osteomalacosis by means of lactic acid. They have given special prominence to the fact that the lime-salts held in solution by the lactic acid must be deposited on neutralization of the acid by the alkaline blood. This objection is not very important, as the alkaline stream of blood has the prop- erty to a high degree of holding earthy phosphates in solution, which can be easily proved. In rachitis the amount of organic substance has been found to vary between 664 and 811 p. m. The quantity of inorganic substance was 189-336 p. in. In opposition to rachitis, osteomalacosis is often characterized by a consider- able amount of fat in the bones, 230-290 p. m. ; but as a rule the composition varies so much that the analyses are of little value. The tooth-structure is nearly related, from a chemical stand- point, to the bony structure. Of the three chief constituents of the teeth, dentin, enamel, and cement, the last-mentioned, the cement, is to be considered as true bony structure, and as such has been spoken of to a certain extent. Dentin has the same composition as the bony structure, 242 PHYSIOLOGICAL CHEMISTRY. but contains somewhat less water. The organic substance yields gelatine on boiling; but the dental tubes are not dissolved, there- fore they cannot consist of collagen. In dentin 260-280 p. m. organic substance has been found. Enamel is an epithelium for mation containing a large proportion of lime-salts. The fact that the organic substance of enamel does not yield any gelatine corre- sponds to its nature and origin. Completely-developed enamel contains the least water, the greatest quantity of mineral sub- stances, and is the hardest of all the tissues of the body. In full- grown animals it contains hardly any water, and the amount of organic substance amounts to only 20-40 p. m. The relative amounts of calcium and phosphoric acid are, according to the analyses of HOPPE-SEYLER, about the same as in bone-earths. According to the determination of BERZELIUS, the enamel may contain 40 p. m. calcium fluoride. IV. The Fatty Tissue. The membranes of the fat-cells withstand the action of alcohol and ether. They are not dissolved by acetic acid nor by dilute mineral acids, but are dissolved by artificial gastric juice. They may possibly consist of a substance closely related to elastin. The contents of the fat-cells are fluid during life, but solidify after death and become more or less solid, depending upon the charac- ter of the fats. Besides fat, the fat-cells contain a yellow pigment which in emaciation does not disappear so rapidly as the fat; and this is the reason that the subcutaneous cellular tissue of an ema- ciated corpse has a dark orange-red color. The cells deficient in or nearly free from fat, which remain after the complete disappear- ance of the latter, seem to have an albuminous protoplasm rich in water. The less water the fatty tissue contains, the richer it is in fat. SCHULTZE and KEINECKE found in 1000 parts : Water. Membrane. Fat. Fatty tissue of oxen 99.6 11.6 888.8 « " "sheep 104.8 16.4 878,8 " "pigs 64.4 13.5 922.1 TISSUES OF THE CONNECTIVE SUBSTANCE. 243 The fat contained in the fat-cells consists chiefly of triglycerides of stearic, palmitic, and oleic acids. Besides these, especially in the less solid kinds of fat, there are glycerides of caproic, valerianic, and other fatty acids which have not been so closely investigated! In all animal fats there are besides these, as HOFMANX has shown,' free, non-volatile fatty acids, although in very small amounts! Fats from different species of animals, and even from different parts of the same animal, have an essentially different consistency, depending upon the relative amounts of the different fats. In solid fats— as tallow— tristearin and tripalmitin are in excess, while the less solid fats are characterized by a greater abundance of palmitin and triolein. This last-mentioned fat is found in greater quantities proportionally in cold-blooded animals, and this accounts for the fat of these animals remaining fluid at temperatures at which the fat of warm-blooded animals solidifies. Human fat from different organs and tissues contains, in round numbers, 670- 800 p. m. olein. The melting-point of different fats depends upon the composition of the mixtures, and it not only varies for fat from different tissues of the same animal, but also for the fat from the same tissues in various kinds of animals. Fat occurs in all organs and tissues of the animal organism, though the quantity may be so variable that a tabular exhibit of the amount of fat in different organs is of little interest. The marrow contains the largest quantity proportionally, having over 960 p. m. The three most important deposits of fat in the animal organism are the intermuscular connective tissue, the fatty tissue in the abdominal cavity, and the subcutaneous connective tissues. The average composition of animal fat is as follows : C 76.5, H 12.0, and 0 11.5 per cent. Neutral fats are colorless or yel- lowish and, when perfectly pure, odorless and tasteless. They are lighter than water, on which they float when in a molten condi- tion. They are insoluble in water, dissolve in boiling alcohol, but separate on cooling, — often in crystals. They are easily soluble in ether, benzol, and chloroform. The fluid neutral fats give an emulsion when shaken with a solution of gum or albumin. With water alone they give an emulsion only after vigorous and pro- longed shaking, but the emulsion is not persistent. The presence of some soap causes a very fine and permanent emulsion to form 244 PHYSIOLOGICAL CHEMISTRY. easily. Fat produces spots on paper which do not disappear ; it is not volatile ; it boils at about 300° C. with partial decomposi- tion, and burns with a luminous and smoky flame. The fatty acids have most of the above-mentioned properties in common with the neutral fats, but differ from them in being soluble in alcohol-ether,, in having an acid reaction, and by not giving the acrolein test. The neutral fats generate a strong irritating vapor of acrolein, due to the decomposition of glycerine, C3H5(OH)3 — 2H20 = C3H40, when heated alone, or more easily when heated with potassium bisulphate or with other substances removing water. The neutral fats may be split by the addition of the constitu- ents of water according to the following equation : C3H5(OR)3 -}- 3H20 = C3H5(OH)3 + 3HOR. This splitting may be produced by the pancreatic enzyme or by superheated steam. We most fre- quently decompose the neutral fats by boiling them with caustic alkali not too concentrated, or, still better (in zoochemical re- searches), with an alcoholic potash solution. By this procedure, which is called saponification, the alkali salts of the fatty acids (soaps) are formed. If the saponification is made with lead oxide, then lead-plaster, lead-salts of the fatty acids, is produced. Stearin, or TEISTEARIK, C3H5(C18H3ft02)3, occurs especially in the solid varieties of tallow, but also in the vegetable fats. Stearic acid, C^H^Ca, is found in the free state in decomposed pus, in the expectorations in gangrene of the lungs, and in cheesy tuberculous masses. It occurs as lime-soap in excrements and adi- pocere, and in this last product also as an ammonia soap. It per- haps exists as sodium soap in the blood, transudations, and pus. Stearin is the hardest and most insoluble of the three ordinary neutral fats. It is nearly insoluble in cold alcohol and soluble with great difficulty in cold ether (225 parts). It separates from warm alcohol on cooling as rectangular, less frequently as rhombical plates. In regard to the melting-point, which may be changed by alternately warming and cooling, opinions are somewhat divergent; for stearin from the fatty tissues it is often stated as 63° C. Stearic acid crystallizes (on cooling from boiling alcohol) in large, shining, long- rhombical scales or plates. It is less soluble than the other fatty acids and melts at 69.2° C. Its barium salt contains 19.49$ barium. TISSUES OF THE CONNECTIVE SUBSTANCE. 245 Palmitin, TRIPALMITIN, 03H6(OH3i08)3. Of the two solid varieties of fats, palmitin is the one which occurs in predominant quantities in human fat (LANGER). Palmitin is present in all animal fats and in several kinds of vegetable fats. A mixture of sterin and palmitin was formerly called MARGARIN.* Palmitic acid, C16H3802. As to occurrence about the same remarks apply as to stearic acid. The mixture of these two acids has been called margaric acid, and this mixture occurs — often as very long, thin, crystalline plates — in old pus, in expectorations from gang- rene of the lungs, etc. Palmitin crystallizes, on cooling from a warm saturated solution in ether or alcohol, into starry rosettes of fine needles. The mix- ture of palmitin and stearin, called margarin, crystallizes, on cool- ing from a solution, as balls or round masses which consist of short or long, thin plates or needles which often appear like blades of grass. Palmitin, like stearin, has a variable melting and solidify- ing point, depending upon the way it has been previously treated. The melting-point is often given as-f 62° and the solidifying-point as + 45° C. Palmitic acid crystallizes from an alcoholic solution in tufts of fine needles. It melts at -f- 62° C.; still the admixture with stearic acid, as HEINTZ has shown, essentially changes the melting and solidifying points according to the relative amounts of the two acids. Palmitic is somewhat more soluble in cold alcoliol than stearic acid ; but they have about the same solubility in boiling alcohol, ether, chloroform, and benzol. Olein, TRIOLEIN, CsH^CwHasO.^ , is present in all animal fats and in greater quantities in plant fats. It is a solvent for stearin and palmitin. Oleic acid, ELAIC ACID, C^HaA, occurs probably as soaps in the intestinal canal during digestion and in the chyle. Olein is, at ordinary temperatures, a nearly colorless oil of a specific gravity of 0.914, without odor or marked taste. It solidifies in crystalline needles at — 5° C. It becomes rancid quickly if exposed to the air. It dissolves with difficulty in cold alcohol, but more easily in warm alcohol or in ether. It is converted into its isomer, ELAIDI^, by nitrous acid. i This mixture must not be confounded with the synthetically-prepared neutral fat called trimargarin. 246 PHYSIOLOGICAL CHEMISTRY. Oleic acid forms at ordinary temperature a colorless, tasteless, and odorless oily liquid which crystallizes at about + 4° C. On being heated it yields, besides volatile fatty acids, SEBACIC ACID, C10H180±, which crystallizes in shining plates and melts at -J- 127° C. Oleic acid is converted by nitrous acid into its isomer, ELAIDIC ACID, which is a solid, melting at +45° C. Oleic acid is insoluble in water but dissolves in alcohol, ether, and chloroform. With con- centrated sulphuric acid and some cane-sugar it gives a beautiful red or red dish- violet liquid whose color is similar to that produced in PETTENKOFER'S test for bile-acids. If the watery solution of the alkali combinations of oleic acid is precipitated with lead acetate, a white, tough, sticky mass of oleate of lead is obtained which is not soluble in water and only slightly in alcohol, but is soluble in ether (differing from the lead-salts of the other two fatty acids). An acid related to oleic acid, DOGLINGIC ACID, which is solid at 0° C., liquid at -\- 16°, and soluble in alcohol, is found in the blubber of the BAL^ENA ROSTRATA. To detect the presence of fat in an animal fluid or tissue the fat must first be extracted with ether. After the evaporation of the ether the residue is tested for fat and the acrolein test must not be neglected. If this test gives positive results, then neutral fats are present; if the results are negative, then only fatty acids are present. If the above residue after evaporation gives the acrolein test, then a small portion is dissolved in alcohol-ether free from acid and which has been colored bluish violet by tincture of alkanet. If the color becomes red, a mixture of neutral fat and fatty acids is present. In this case the fat is treated in the warmth with a soda solution and evaporated on the water-bath, constantly stirring until all the water is removed The fatty acids are hereby combined with the alkali as soaps, while the neutral fats are not saponified under these con- ditions. If this mixture of soaps and neutral fats is treated with water and then shaken with pure ether, the neutral fats are dis- solved, while the soaps remain in the watery solution. The fatty acids may be separated from this solution by the addition of a mineral acid which sets the acid free. The neutral fats separated from the soaps by means of ether are contaminated with some cholesterin, which must be separated in quantitative determinations by saponification with alcoholic caustic potash. The cholesterin is not attacked by the caustic alkali while the neutral fats are saponified. After the evaporation of the alcohol TISSUES OF THE CONNECTIVE SUBSTANCE. 247 the residue is dissolved in water and shaken with ether, which dis- solves the cholesterin. The fatty acids are separated from the watery solution of the soaps by the addition of a mineral acid. If a mixture of soaps, neutral fats, and fatty acids is originally present, it is treated first with water, then agitated with ether free from alcohol, which dissolves the fat and fatty acids, while the soaps remain in the solution, with the exception of a very small amount which is dissolved by the ether. To detect and to separate the different varieties of neutral fats from each other it is best first to saponify them with alcoholic potash. After the evaporation of the alcohol they are dissolved in water and precipitated with sugar of lead. The oleate of lead is then separated from the other two lead-salts by repeated extraction with ether. The residue insoluble in ether is decomposed on the water- bath with an excess of soda solution, evaporated to dryness, finely pulverized, and extracted with boiling alcohol. The alcoholic solution is then fractionally precipitated by barium acetate or barium chloride. In one fraction the amount of barium is deter- mined, and in the other the melting-point of the fatty acid set free by a mineral acid. The fatty acids occurring originally in the ani- mal tissues or fluids as free acids or as soaps are converted into barium salts and investigated as above. The derivation of the fats in the organism may occur in various ways. The fat of the animal body may consist partly of absorbed fat of the food deposited in the tissues and partly of fat formed in the organism from other bodies, such as albuminous bodies or carbohydrates. That the fat of the food which is absorbed in the intestinal canal may be retained by the tissues has been shown in several ways. LEBEDEFF and MUNK have fed dogs with various fats, such as linseed-oil, mutton-tallow, and rape-seed-oil, and have afterwards found the administered fat in the tissues. HOFMANN starved dogs until they appeared to have lost their fat, and then fed them upon large quantities of fat and only little proteids. When the animals were killed he found so large a quantity of fat that it could not have been formed from the administered proteids alone, but the greatest part must have been derived from the fat of the food. PETTENKOFER and VOIT arrived at similar results in regard to the behavior of the absorbed fats in the organism, though their experi- ments were of another kind. MUNK has found that on feeding with free fatty acids, these are deposited in the tissues, not, how- 248 PHYSIOLOGICAL CHEMISTRY. ever, as such; but they are transformed by synthesis with glycerin into neutral fats on their passage from the intestines to the thoracic duct. According to EWALD, such a synthesis may be pro- duced by the surviving mucous membrane of the intestine. Albuminous bodies and carbohydrates may be considered as the mother-substance of the fats formed in the organism. The formation of the so-called COBPSE-WAX, ADIPOCEBE, into which parts of the corpse rich in proteids are sometimes converted, and which consists of abundant quantities of fatty acids, ammonia, and lime-soaps, is often given as a proof of the formation of fats from proteids. The provableness of this observation has, however, been disputed, and many other explanations of the formation of this substance have been offered. According to the recent experi- ments of KBATTEB and K. B. LEHMANN, it seems as if it were possible by experimental means to convert animal tissue rich in proteids (muscles) into adipocere by the continuous action of water. That the formation of fatty acids in this case actually takes place at the expense of the proteids may be inferred from the investiga- tions of LEHMANI*. Another proof of the formation of fat from proteids, taken from pathological chemistry, is fatty degeneration. On this point also all investigators are not united; but the investigations of BAUEB seem to show that at least in acute poisoning by phosphorus the fatty degeneration actually consists of a formation of fat from proteids. As a more direct proof of fat-formation from proteids the inves- tigations of PETTE^KOFEB and VOIT are often quoted. These investigators fed dogs with large quantities of meat containing the least possible proportion of fat, and found all of the nitrogen in the excreta but only a part of the carbon. As an explanation of these conditions it has been assumed that the proteid of the organism splits into a nitrogenized and a non-nitrogenized part, the former changing into the nitrogenized final product, urea, the other, on the contrary, being retained in the organism as fat (PET- TENKOFEB and VOIT). Another more direct proof of a fat formation from proteid has been given by HOFMANN. He experimented with fly-maggots. A number of these were killed and the quantity of fat determined. TISSUES OF THE CONNECTIVE SUBSTANCE. 249 The remainder were allowed to develop in blood whose proportion of fat had been previously determined, and after a certain time they were killed and analyzed. He found in them from 7 to 19 times as much fat as the maggots first analyzed and the blood together contained. While, then, in view of this experiment, the question of the formation of fat from proteids can hardly be disputed, we do not yet know the maximum amount of fat formed from the proteids nor the chemical processes concerned in its formation. DRECHSEL, mindful of the products which are formed by the decomposition of albumin with barium hydrate, has called attention to the fact that the albumin molecule probably originally contains no radical with more than six or nine carbon atoms. If fat is formed from albu- min in the animal body, then, according to DRECHSEL, such forma- tion is not a splitting off from the albumin, but rather a synthesis from primarily-formed splitting products of albumin which are deficient in carbon. The theory of the formation o fat from carbohydrates in the animal body was first adopted by LIEBIG. This was combated for some time, however, and until lately it was the general opinion that a direct formation of fat from carbohydrates had not been proved, and also that it was improbable. The undoubtedly great influence which LIEBIG has shown the carbohydrates to exert on the forma- tion of fat has been explained by C. Y. VOIT upon the assumption that these carbohydrates are consumed instead of the fat absorbed or formed from the albumins, and therefore have an action tending to economize the fat. By means of a series of nutrition tests with foods especially rich in carbohydrates, LA WES and GILBERT, SOXHLET, TSCHERWINSKY, MEissL and STROMER (on pigs), B. SCHULTZE, CHANIEWSKI, E. VOIT and C. LEHMANN (on geese), J. MUNK and M. RUBBER (on dogs) apparently prove that a direct formation of fat from carbohydrates does actually occur. The processes by which this formation takes place are still unknown. As the carbohydrates do not contain as complicated a molecule as the fats, the formation of fat from carbohydrates must consist of a synthesis, in which, the group CHOH is converted into CH2, a reduction must also take place. When food contains an excess of fat, the superfluous amount is 250 PHYSIOLOGICAL CHEMISTRY. stored np in the fatty tissue, and on partaking of food deficient in fat this accumulation is quickly exhausted. There is perhaps no one of the various tissues that decreases so much in starvation as the fatty tissue. The organism, then, possesses in this tissue a depot where there is stored during proper alimentation a substance of great importance in the development of heat and vital force, which substance, on insufficient nutrition, is given off as may be needed. The fatty tissues, on account of their low conducting power, become of great importance in regulating the loss of heat from the body. They also serve to fill cavities, and as a protection and sup- port to certain internal organs. Appendix to the Fatty Tissue. Spermaceti. In the living spermaceti or white whale there is found in a large cavity in the skull an oily liquid called spermaceti, which on cooling after death separates into a solid crystalline part, ordinarily called SPERMACETI, and into a liquid, SPERMACETI-OIL. This last is separated by pressure. Sper- maceti is also found in other whales and in certain species of dolphin. The purified, solid spermaceti, which is called CETIN, is a mixture of esters of fatty acids. The chief constituent is the cetyl palmitic ester mixed with small quantities of compound ethers of lauric, myrisitic, and stearic acids with radicals of the alcohols, LETHAL, Cj2Ha6.OH, METHAL, Ci4H29.OH, and STETHAL, Ci8H37.OH. Cetin is a snow-white mass shining like mother-of-pearl, crystallizing in plates, brittle, fatty to the touch, and which has a varying melting-point of _|_ 30° to 50° C., depending upon its purity. Cetin is insoluble in water, but dissolves easily in cold ether or volatile and fatty oils. It dissolves in boiling 'alcohol, but crystallizes on cooling. It is saponified with difficulty by a solu- tion of caustic potash in water, but with an alcoholic solution it saponifies readily and the above-mentioned alcohols are set free. Ethal, or cetyl alcohol, Ci6H33. OH, which also occurs in the coccygeal gland of ducks and geese (DE JONGE) and in smaller quantities in beeswax, forms white, transparent, odorless, and tasteless crystals which are insoluble in water but dissolve easily in alcohol and ether. ^ Ethal melts at 49.5° C. SPERMACETI-OIL yields on saponification valerianic acid, small amounts of solid fatty acids, and PHYSETOLEIC ACID. This acid forms colorless and odor- less, needle-shaped crystals which easily dissolve in alcohol and ether and melt at + 34° C. BEESWAX may be treated here as concluding the subject of fats. It con- tains three chief constituents. 1. CEROTIC ACID, C27H64O2 , which occurs as cetyl ether in Chinese wax and as free acid in ordinary wax. It dissolves in boiling alcohol and separates as crystals on cooling. The cooled alcoholic extract of wax contains (2) CEROLEIN, which is probably a mixture of several bodies, and (3) MYRISIN, which forms the chief constituent of that part of wax which is insoluble in warm or cold alcohol. Myrisin consists chiefly of palmitic-acid ether of melissyl (myricyl) alcohol, C«efi«.OH. This alcohol is a silky, shining, crystalline body melting at -f 85° C. CHAPTER IX. MUSCLE. Striated Muscles. IN the study of the muscles the chief problem for physiological chemistry is to isolate their different morphological elements and to investigate each element separately. By reason of the compli- cated structure of the muscles this has been thus far almost impos- sible, and we must be satisfied at the present time with the investi- gation of the chemical composition of the muscular fibres and with a few micro-chemical reactions. Each muscle-tube or muscle-fibre consists of a sheath, the SARCOLEMMA, which apparently is composed of a substance similar to elastin, and the contents containing a large proportion of ALBUMINS. This last-mentioned substance, which in life is- possessed of contractile force, has in the inactive muscle an alkaline reaction, or, more correctly speaking, an amphoteric reaction with a predomi- nating action on red litmus-paper. This reaction depends on a mixture of mono- and di-potassium phosphate with a preponderance of diphosphate. The dead muscle has, on the contrary, an acid reaction depending on the presence of potassium monophosphate and free lactic acid. If we disregard the disputed statements relative to the finer structure of the muscles, we can differentiate in the striated muscles between the two chief components, the doubly refracting — aniso- tropous — and the singly refracting — isotropous — substance. If the muscular fibres are treated with reagents which dissolve albumins, such as dilute hydrochloric acid, soda solution, or gastric juice, they swell greatly and break up into " BOWMAN'S disks." By the action of alcohol, chromic acid, boiling water, or in general such 251 252 PHYSIOLOGICAL CHEMISTRY. reagents as cause a shrinking, the fibres split longitudinally into fibrils ; and this behavior shows that several chemically different substances of various solubilities enter into the construction of the muscular fibres. The albuminous body, myosin, is generally considered as the principal constituent of the diagonal disks, consisting of a doubly- refracting substance, while the chief mass of the remaining albumin- ous bodies found in the muscles, also most of the extractive matter, is contained in the isotropous substance. According to the obser- vations of DAJTILEWSKY, myosin may be completely extracted from the muscle without changing its structure, by the use of a 5$ solu- tion of ammonium chloride. This is contrary to the above state- ments. DANILEWSKY claims that in the structure of the muscles another substance enters, similar to albumin, which has not been closely studied and which does not dissolve in ammonium chloride solution, but only expands. Albuminous bodies constitute an important part of the muscular structure, of which it forms the chief mass of its solids. Albuminous Bodies of the Muscles. Like the blood which contains a fluid, the blood-plasma, which spontaneously coagulates, separating fibrin and yielding blood -serum, so also the living muscle contains, as first shown by KUHNE, a spontaneously coagulating liquid, the muscle-plasma, which coagu- lates quickly, separating an albuminous body, myosin, and yielding also a serum. That liquid which is obtained by pressing the living muscle is called muscle-plasma, while that obtained from the dead muscle is called muscle-serum. These two fluids also con- tain different albuminous bodies. The muscle-plasma was first prepared by KimtfE from frog- muscles, and lately HALLIBURTON has prepared it according to the same method from the muscles of warm-blooded animals, especially rabbits. The principle of this method is as follows : The blood is removed from the muscles immediately after the death of the animal by passing through them a strongly-cooled common-salt solution of 5 to 6 p. m. Then the quickly-cut muscles are immediately thoroughly frozen so that they can be ground in this state to a fine mass — " muscle-snow." This pulp is strongly pressed in the cold, and MUSCLE. 253 the liquid which drips off, the muscle-plasma, is faintly yellowish in color, alkaline, and spontaneously but slowly coagulates at a little above 0° C., but very quickly at the temperature of the body. In the muscle-plasma of the frog the reaction does not change imme- diately with the coagulation, but the alkaline reaction is gradually changed into an acid one. The liquid which is pressed from tfte clot, the muscle-serum, is always acid. The albuminous body which forms the clot has been called myosin. Besides this, another albuminous body, musculin or paramyosin (HALLIBURTON), is found in the clot. Myosin was first discovered by KUHNE, and constitutes the principal mass of the albuminous bodies of the dead muscle, and according to a few investigators it forms the greatest part of the contractile protoplasma. The statements as to the occurrence of myosin in other organs besides the muscles require further proof. The amount of myosin in the muscles of different animals varies, according to DANILEWSKY, between 30-110 p. m. Myosin is a globulin whose elementary composition, according to CHITTENDEN and CUMMINS, is, on an average, the following : C 52.82, H 7.11, N 16.17, Sl.27, 022.03 per cent. If the myosin separates as fibres, or if a myosin solution with a minimum quantity of alkali is allowed to evaporate on a microscope-slide to a gelatinous mass, doubly-refracting myosin may be obtained. Myosin has the general properties of the globulins. It is completely pre- cipitated by saturating with NaCl, also by MgSO*, in a solution containing 94$ of the salt with its water of crystallization (HALLI- BURTON). Like fibrinogen it coagulates at -}- 56° C. in a solution containing common salt, but differs from it since under no circum- stances can it be converted into fibrin. The coagulation tempera- ture, according to CHITTENDEN and CUMMINS, not only varies for myosin of different origin, but also for the same myosin in different salt solutions. Myosin may be prepared in the following way, as suggested by HALLIBURTON. The muscle is first extracted by a 5$ magnesium sulphate solution. The filtered extract is then treated with mag- nesium sulphate fa substance until 100 cc. of the liquid contains about 50 grms. of the salt. The so-called paramyosin or musculin separates. The filtered liquid is then treated with magnesium 254 PHYSIOLOGICAL CHEMISTRY. sulphate until each 100 cc. of the liquid holds 94 grms. of the salt in solution. The rnyosin which now separates is filtered, dissolved in water by aid of the retained salt, precipitated by diluting with water, and, when necessary, purified by redissolving in dilute-salt solution and precipitating with water. The older and perhaps the usual method of preparation consists, according to DANILEWSKY, in extracting the muscle with a 5-10$ ammonium-chloride solution, precipitating the myosin from the filtrate by strongly diluting with water, redissolving the precipitate in ammonium-chloride solution, and the myosin obtained from this solution is either reprecipitated by diluting with water or by remov- ing the salt by dialysis. As the coagulation of the blood-plasma is considered by most investigators as an enzymotic process, so certain observations seem to show that the coagulation of the muscle-plasma is an analogous process. From muscles which had been kept for a long time in alcohol HALLIBURTON obtained, by extracting the mass with water, a soluble substance contaminated with albumose which, although not identical with fibrin ferment, had the property of accelerating the coagulation of the muscle-plasma. This sub- stance was called by him " myosin-ferment." As in the blood-plasma we have a mother-substance of fibrin, fibrinogen, so also it is considered that in the muscle-plasma we have a mother-substance of myosin, myosinogen. This body has not thus far been isolated with certainty. HALLIBURTON' found that a solution of purified myosin in dilute-salt solution (5$ MgS04), and sufficiently diluted with water, coagulates after a certain time, and at the same time becomes acid and a typical myosin-clot separates. This coagulation, which is accelerated by warming or by the addition of myosin-ferment, is, according to HALLIBURTON, a process analogous to the coagulation of the muscle-plasma. Ac- cording to this same investigator, myosin when dissolved in water by the aid of a neutral salt is reconverted into myosinogen, while after diluting with water myosin is again produced from the myosinogen. These observations may, however, be explained in other ways. In these cases the separation of the myosin is evi- dently closely connected with the liquid becoming acid, while the separation of myosin from the muscle-plasma, at least from the muscle-plasma of the frog, is independent of this acidity, for it may MUSCLE. 255 take place before the liquid becomes acid. The mother-substance of myosin and the chemical processes of the myosin coagulation are questions which must not be considered as settled. Musculin, called PARAMYOSINOGEN by HALLIBURTON, is a glob- ulin which is characterized by its low coagulation temperature, about -f- 47° C., which may vary in different species of animals (-f- 45° in frogs, + 51° C. in birds). It is more easily dissolved than myosin by NaOl or MgSO± (salt containing 50$ water of crystallization). Musculin is separated simultaneously with myosin in the coagulation of the muscle-plasma, and it is therefore found in the clot. A solution which contains musculin and no myosin does not coagulate on the addition of the myosin ferment (HALLI- BURTON). If the dead muscle is extracted with water, the mus- culin passes in part into solution. The musculin may be isolated by fractional precipitation with magnesium sulphate (50 grms. to each 100 cc. liquid), and may be identified by its low coagulation temperature. Myoglobulin. After the separation of the musculin and the myosin from the salt extract of the muscle by means of MgSO± the myoglobulin may be precipitated by saturating the filtrate with the salt. It is similar to serum globulin, but coagulates at -|- 63° C. (HALLIBURTON). Myoalbumin, or muscle-albumin, seems to be identical with serum-albumin (serum-albumin a, according to HAL- LIBURTON), and is .prepared according to the same method. Myo- albumose (a deuteroalbumose) is found in small quantities in the muscles, and may be obtained by extracting with water the finely- divided mass of muscle which has previously been coagulated by keeping in alcohol for a long time (HALLIBURTON). After the complete removal from the muscle of all albuminous bodies which are soluble in water and ammonium chloride, DANI- LEWSKY claims that an insoluble albuminons body remains which only swells in ammonium-chloride solution and which forms with the other insoluble constituents of the muscular fibre the " muscle- stroma." According to DANILEWSKY, the amount of such stroma substance is connected with the muscle activity. He maintains that the muscles contain a greater amount of this substance, com- pared with the myosin present, when the muscles are quickly con- tracted and relaxed. 256 PHYSIOLOGICAL CHEMISTRY. Muscle-syntonin, which may be obtained by extracting the muscles with hydrochloric acid of 1 p. m., and which, according to K. MORNER, is less soluble and has a greater aptitude to precipitate than other acid albumins, seems not to occur pre-existent in the muscles. Muscle-coloring Matters. There is no question that the red color of the muscles even when completely freed from blood de- pends in part on haemoglobin, though it is contested by many. MAcMuNtf claims that the muscles contain also another coloring substance which is nearly related to the blood-coloring matters and whose spectrum is very similar to that of haemochromogen. This coloring matter has been called myoJicematin. According to LEVY and HOPPE-SEYLER, this myohaematin is nothing but haemochromo- gen, which is produced from oxyhaemoglobin by decomposition and reduction. Nevertheless MAcMuNN still adheres to his view that myohsematin is an independent coloring substance, and in sup- port of his opinion he adduces the fact that inyohaematin is found also in the muscles of insects in which no haemoglobin occurs. The reddish-yellow coloring matter of the muscles of the salmon has been little studied. Traces of enzymes, such as pepsin and diastatic enzymes, have been found in them. The so-called " myosin-ferment," and probably an enzyme producing lactic-acid fermentation, are also found in these muscles. Extractive Bodies of the Muscles. The nitrogenized extractives consist chiefly of creatin, in the proportion of 2-4 p. m., in the fresh muscles containing water, alj/o the xanthin bodies, hypoxanthin and xanthin, besides guanin and carnin. The average quantity of hypoxanthin, xanthin, and guanin in 1000 parts of the dried substance of the muscles of oxen is, according to KOSSEL, respectively 2.30, 0.53, and 0.20 grms., and in the embryonic ox-muscles respectively 3.59, 1.11, and 4.12 grms. Besides these we must also consider as an extractive body the syrupy inosic acid (CioHi4N4Oii), of which only traces are found in certain animals. This acid was first prepared by LIEBIG, but not closely studied. LIMPRICHT has found another in the flesh of certain cyprindea, namely, the nitrogenized pro** acid. Uric acid, urea, taurin, and leucin are found as traces in the muscles in certain cases only, in a few species of animals. In regard to the amount of these different extractives in the muscles, KRUKENBERG and WAGNER have shown that it varies greatly in different animals. A large amount of urea is found in the muscles of the shark and ray ; uric acid is found in alligators ; MUSCLE. 257 taurin in cephalopoda ; glycocoll in mollusks, pecteii irradians ; and creatinin in luvarus imperialis, etc., etc. The xanthin bodies, with the exception of carnin, have been treated on pages 47-52, and therefore among the extractive bodies we will first consider the discussion of creatin. Creatin, C4H9N302 -\- H20 or METHYLGUANIDIN-ACETIC ACID, NH:C(NH2).N(CH3).CH2.COOH + H20, occurs in the muscles of vertebrate animals in variable amounts in different species ; the largest amount is found in birds. It is also found in the braia, blood, transudations, and the amniotic fluid. Creatin may be pre- pared synthetically from cyanamid and sarcosine (methylglycocoll). On boiling with baryta-water it decomposes, with the addition of water, and yields urea, sarcosine, and certain other products. Be- cause of this behavior several investigators consider creatin as a step in the formation of urea in the organism. On boiling with acids creatin is easily converted, with the elimination of water, into creatinin, whicli occurs in urine, and which has also been found in the muscles of the dog by MOKARI (see Chapter XIV). Creatm crystallizes in hard, colorless, monoclinic prisms which lose their water of crystallization at 100° C. It dissolves in 74 parts of water at the ordinary temperature and 9410 parts abso- lute alcohol. It dissolves more easily with the aid of heat. Its watery solution has a neutral reaction. Creatin is not dissolved by ether. If a creatin solution is boiled with precipitated mercuric oxide, this is reduced to mercury and oxalic acid, and the disgust- ing-smelling methyluramin (methylguanidin) is developed. A solution of creatin in water is not precipitated by basic-lead acetate but gives a white, flaky precipitate with mercurous nitrate. When boiled for an hour with dilute hydrochloric acid creatin is con- verted into creatinin, and may be identified by its reactions. The preparation and detection of creatin is best performed by the following method of NEUBAUER, which was first used in the preparation of creatin from muscles. Finely-cut flesh is extracted with an equal weight of water at -f- 55° to 60° C. for 10-15 minutes, Eressed and extracted again with water. The albumin is removed rom the united extracts as far as possible by coagulation at boiling heat, the filtrate precipitated by the careful addition of basic-lead acetate, the lead removed from this filtrate by H2S and carefully concentrated to a small volume. The creatin, which crystallizes in 258 PHYSIOLOGICAL CHEMISTRY. a few days, is collected on a filter, washed with alcohol of 88$, and purified, when necessary, by recrystallization. The quantitative estimation of creatin is performed according to the same method. Carnin, CvHsN^Os + H2O, is one of the substances found by WEIDEL in American meat extract. It has also been found by KRTJKENBERG and WAG- NER in frog-muscles and in the flesh of fish, and by POUCHET in urine. As previously stated (page 48), carnin may be converted into hypoxanthin by means of oxidation. Carnin has been obtained as a white crystalline mass. It dissolves with difficulty in cold water, but dissolves easily in warm. It is insoluble in alco- hol and ether. It dissolves in warm hydrochloric acid and yields a salt crys- tallizing in shining needles, which gives a double combination with platinum. chloride. Its watery solution is precipitated by silver nitrate, but this precipi- tate is neither dissolved by ammonia nor by warm nitric acid. Carnin does not give the so-called WEIDEL 's xanthin reaction. Its watery solution is precipitated by basic- lead acetate ; still the lead combinations may be dissolved on boiling. We must also include among the nitrogenized extractives those bodies which were discovered by GAUTIER, and which occur in very small quan- tities, namely, the leucomaines : xanthocreatinin, C6HioN4O, crusocreatinin, , amphicreatin, C9Hi9N7O4, and pseudoxanthin, The non-nitrogenized extractive bodies of the muscles are inosit, glycogen, sugar, and lactic acid. Inosit, C6H1206 + H20. This body, discovered by SCHERER, is not a carbohydrate, but belongs to the aromatic series and seems to be hexahydroxybenzol (MAQUEK^E). With hydroiodic acid it yields benzol and tri-iodophenol, and on oxidation with nitric acid, tetra-oxychinon. Inosit is found in the muscles, liver, spleen, kidneys, super-renal cavity, lungs, brain, testicles, and in the urine in pathological cases. It is found very widely distributed in the vegetable kingdom, especially in unripe fruits, in green beans, (phaseolus vulgaris), and therefore it is also called PHASEOMAKKIT. Inosit crystallizes in large, colorless, rhombical crystals of the monoclinic system, or, if not pure and if only a small quantity crystallizes, it forms groups of fine crystals similar to cauliflower. It looses its water of crystallization at 110° C., also if exposed to the air for a long time. Such exposed crystals are non-transparent and milk-white. The crystals melt at 217° 0. Inosit dissolves in six parts of water at ordinary temperature, and the solution has a sweetish taste. It is insoluble in strong alcohol and in ether. Inosit does not ferment with beer-yeast; it dissolves copper oxyhy- drate in alkaline solutions, but does not reduce on boiling. It gives negative results with MOORE'S or BOTTGER-ALMEST'S bismuth tests. MUSCLE. 259 If inosit is evaporated to dryness on a platinum foil with nitric acid and the residue treated with ammonia and a drop of calcium- chloride solution and carefully re-evaporated to dryness, a beautiful rose-red residue is obtained (SCHERER'S inosit test). If we evapo- rate an inosit solution to incipient dryness and moisten the residue with a little mercuric-nitrate solution, we obtain a yellowish residue on drying, which becomes a beautiful red on strongly heating. The coloration disappears on cooling, but it reappears on gently warming (GALLOIS'S inosit test). To prepare inosit from a liquid or from a watery extract of a tissue, the albumin is first removed by coagulating at boiling heat. The filtrate is precipitated by sugar of lead, this filtrate boiled with basic-lead acetate and allowed to stand 24-48 hours. The precipi- tate thus obtained, which contains all the inosit, is decomposed in water by H2S. The filtrate is strongly concentrated, treated with 2-4 vols. hot alcohol, and the liquid removed as soon as possible from the tough or flaky masses which ordinarily separate. If no crystals separate from the liquid within 24 hours, then treat with ether until the liquid has a milky appearance and allow it to stand. In the presence of a sufficient quantity of ether, crystals of inosit separate within 24 hours. The crystals thus obtained, as also those which are obtained from the alcoholic solution directly, are recrystallized by redissolving in very little boiling water and the addition of 3-4 vols. alcohol. Glycogen is a constant constituent of the living muscle, while it may be absent in the dead muscle. The amount of glycogen varies in the different muscles of the same animal. BOHM found 10 p. m. glycogen in the muscles of cats, and moreover he found a greater amount in the muscles of the extremities than in those of the rump. GRUTZNER obtained more glycogen from the white muscles of mammalia than from the red ones. The food also has a great influ- ence. BOHM found 1-4 p. m. glycogen in the muscles of fasting animals and 7-10 p. m. after partaking of food. LUCHSINGER maintains an opinion, formerly generally accepted, that in starvation, or if there is a lack of carbohydrates in the food, glycogen disap- pears more quickly from the muscles than from the liver; but ac- cording to ALDEHOFF, exactly the reverse takes place. The glyco- gen disappears more quickly in starvation from the liver than from the muscles, not only in hens, as observed by WEISS, but also in other animals, such as the pigeon, rabbit, cat, and horse. 260 PHYSIOLOGICAL CHEMISTRY. Muscle-sugar, of which traces only occur in the living muscle and which is probably formed after the death of the muscle from the muscle-glycogen, is perhaps grape-sugar (glucose). As an inter- mediate step in this sugar formation we must mention dextrin, which is sometimes found in the muscles. Perhaps this dextrin has been confounded with glycogen. Lactic Acids. Of the four acids of the formula C3H603 there is one, hydracrylic acid, which is not found in the animal organism. The statements as to the occurrence of another, ethylen lactic acid (in meat extract), are disputed. EtUidene lactic acid, CH3 . CH(OH).COOH, is of physiologico-chemical importance. Two modifications occur — the optically inactive FERMENTATION" LACTIC ACID, and the optically active, dextro-gyrate PABALACTIC ACID, Or SARCOLACTIC ACID. The fermentation lactic acid, which is formed from milk-sugar by allowing milk to sour and by the acid fermentation of other carbohydrates, is considered to exist in small quantities in the muscles (HEINTZ), in the gray matter of the brain (GSCHEIDLEN), and in diabetic urine. During digestion this acid is also found in the contents of the stomach and intestines, and as alkali lactate in the chyle. The paralactic acid is, at all events, the true acid of meat extracts, and this alone has been found with certainty in dead muscle. That lactic acid which is found in the spleen, lymphatic glands, thymus, thyroid gland, blood (traces), bile, pathological transudations, osteomalicous bones, in perspiration in puerperal fever, and in the urine after excessive marches, in acute yellow atrophy of the liver, in poisoning by phosporus, especially after extirpation of the liver (in geese, according to MINKOWSKI, in frogs by MARCUSE and WERTHER), seems to be paralactic acid. The lactic acids are amorphous. They have the appearance of colorless or faintly yellowish, acid-reacting syrups which mix in all proportions with water, alcohol, or ether. The salts are soluble in. water, and most of them in alcohol. The two acids are differenti- ated from each other by their different optical properties — paralactic acid being dextro-gryate, while fermentation lactic acid is optically inactive — also by their different solubilities and the different amounts of water of crystallization of the calcium and zinc salts. The zinc salt of fermentation lactic acid dissolves in 58-63 parts of MUSCLE. 261 water at 14°-15° C. and contains 18.18$ water of crystallization, cor- responding to the formula Zn(C3H603)2 -f 3H20. The zinc salt of paralactic acid dissolves in 17.5 parts of water at the above temper- ature and contains ordinarily 12.9$ water, corresponding to the formula Zn(C3H503)2 -f- 2H20. The calcium salt of fermentation lactic acid dissolves in 9.5 parts water and contains 29.22$ (=5 rnol.) water of crystallization, while the calcium paralactate dissolves in 12.4 parts water and contains 24.83 or 26.21$ (=4or4| mol.) water of crystallization. Both calcium salts crystallize, not unlike tyrosin in spheres or tufts of very fine microscopic needles. Lactic acids may be detected in organs and tissues in tlie follow- ing manner: After complete extraction with water the albumin is removed by coagulation at boiling temperature and the addition of & small quantity of sulphuric acid. The liquid is then exactly neutralized while boiling with caustic baryta, and then evaporated to a syrup after filtration. The residue is precipitated with absolute alcohol, and the precipitate completely extracted with alcohol. The alcohol is entirely distilled from the united alcoholic extracts, and the neutral residue is shaken with ether to remove the fat. The residue is taken up by water and phosphoric acid added, and repeatedly shaken with fresh quantities of ether, which dissolve the lactic acid. The ether is now distilled from the several ethereal extracts, the residue dissolved in water, and this solution carefully warmed on the water-bath to remove the last traces of ether and volatile acids. A solution of zinc lactate is prepared from this filtered solution by boiling with zinc carbonate, and this is evapo- rated until crystallization commences and then allowed to stand over sulphuric acid. Fat is never absent in the muscles. Some fat is always found in the inter-muscular connective tissue; but the muscle-fibres them- selves also contain fat. The amount of fat in the real muscle sub- stance is always small, usually amounting to about 10 p. m. or somewhat more. A considerable amount of fat in the muscle-fibres is only found in fatty degeneration. Lecithin is also habitually found in the muscles. The Mineral Bodies of the Muscles. "We have no complete analyses of the mineral substances of the pure, blood-free muscle substance. The ash remaining after burning the muscle, which amounts to about 10-15 p. m., calculated on the moist muscle, is acid in reaction. The largest constituents are potassium and phos- 262 PHYSIOLOGICAL CHEMISTRY. phoric acid. Next in amount we have sodium and magnesium, and lastly calcium, chlorine, and iron oxide. Sulphates only exist as traces in the muscles, but are formed by the burning of the proteids of the muscles, and therefore occur in abundant quantities in the ash. The muscles contain such a large quantity of potassium and phosphoric acid that potassium phosphate seems to be unquestion- ably the predominating salt. Chlorine is found in such insignifi- cant quantities that it is perhaps derived from a contamination with blood or lymph. The quantity of magnesium is about double that of calcium. These two bodies, as well as iron, occur only in very small amounts. The gases of the muscles consist of large quantities of carbon dioxide, besides traces of nitrogen. Rigor Mortis of the Muscles. If the influence of the circulating oxygenated "blood is removed from the muscles, as after death of the animal or by tying the aorta or the muscle-arteries (STEP- SON'S test), rigor mortis, sooner or later takes place. The ordinary rigor appearing under these circumstances is called the spontaneous or the fermentive rigor, because it seems to depend in part on the action of an enzyme. A muscle may also become stiff for other reasons. The muscles may become momentarily stiff by warming, in the case of frogs to 40°, in mammalia to 48°-50°, and in birds to 53° C. (heat-rigor). Distilled water may also produce a rigor in the muscles (water-rigor). Acids, even when very weak, such as carbon dioxide, may quickly produce a rigor (acid-rigor), or hasten its appearance. A number of chemically different substances, such as chloroform, ether, alcohol, ethereal oils, caff em, and many alka- loids, produce a similar effect. That rigor which is produced by means of acids or other agents which, like alcohol, coagulate albumin must be considered as produced by entirely different pro- cesses than the spontaneous rigor. The time within which the spontaneous rigor occurs depends upon the temperature; a low temperature retarding and a high temperature hastening its appearance. Muscular activity also exer- cises an appreciable influence on the rigor of the muscles, for a previous active contraction accelerates the rigor of the muscles; the mechanical abuse. of the muscles acts in the same way. The appear- ance of spontaneous rigor is under the influence of the central nerv- MUSCLE. 263 ous system, and a muscle whose nerve has been severed stiffens more slowly than one whose continuity with the central nervous system has not been destroyed (HERMANN and his pupils v. EISEL- BERG, v. GENDER and BIERFREUND). The nervous system seems also to have a similar influence on the post-mortem acidification of the muscles (GROSS). HERMANN and his pupils consider the rigor mortis as a slowly-proceeding muscular contraction, identical with the ordinary muscular contraction, but it is difficult at this time to determine as to the correctness of this view from a chemical standpoint. When the muscle passes into rigor mortis it becomes shorter and thicker, harder and non-transparent, less ductile and acid. The chemical processes which take place in this step are the following : By the coagulation of the plasma a myosin-clot is produced which is the cause of the hardening and of the diminished transparency of the muscle. The appearance of this clot may be hastened by the simultaneous occurrence of lactic acid. Carbon dioxide is also formed, which does not seem to be a direct oxidation product. HERMANN claims that carbon dioxide is produced in the removed muscle, even in the absence of oxygen, when it passes into rigor mortis. The quantity of carbon dioxide and lactic acid produced on the acidification of the muscle is not dependent upon the quick- ness or slowness of the rigor mortis (RANKE, HERMANN). It is not known from what mother-substance these acids are formed. The most probable explanation is that carbon dioxide and lactic acid are produced from glycogen, and the fact that the glycogen in the muscle decreases on stiffening has been asserted positively (NASSE, WERTHER). On the other side, BOHM has shown that cases are found in which the glycogen is not diminished on stiffening, and he has also found that the quantity of lactic acid produced is not pro- portional to the quantity of glycogen. Under these circumstances, as the muscles of starving pigeons, which are free from glycogen, yield, according to DAM A NT, lactic acid after death, it is hardly possible that the two above acids are formed from glycogen. The only remaining explanation is that they originate from the proteids or certain other not well-known constituents of the muscles. After the muscles have been stiff for some time they relax again and become softer. This may partly depend on their becom- 264 PHYSIOLOGICAL CHEMISTRY. ing strongly acid and the myosin-clot dissolving in the acid, and partly, and in all probability mainly, upon a putrefaction. Exchange of Material in the Inactive and Active Muscles. It is admitted by a number of prominent investigators, PFLUGER and COLASANTI, ZUNTZ and ROHRIG, and others, that the exchange of material in the muscles is regulated by the nervous system. When at rest, when there is no mechanical exertion, we have a condition which ZUNTZ and ROHRIG have designated "chemical tonus" This tonus seems to be a reflex tonus, for it may be re- duced by discontinuing the connection between the muscles and the central organ of the nervous system by cutting through the spinal marrow or the muscle -nerves, or by paralyzing the same by means of curara poison. It may also be reduced or checked by adjusting the temperature between the skin and the surrounding medium; or it maybe increased by the reverse, by irritating the nerves of the skin by cooling. The possibility of reducing the chemical tonus of the muscles by any of the above-mentioned means, but especially by the action of curara, offers an important means of deciding the extent and kind of chemical processes going on in the muscles when at rest. In comparative chemical investi- gation of the processes in the active and in the inactive muscles several ways of procedure have been adopted. The removed homologous, active and inactive muscles have been compared, also the arterial and venous muscle-blood in rest and in activity, and lastly the total exchange of material, the receipts and expenditures of the organism, have been investigated under these two conditions. By investigations according to these several methods, it has been found that the active muscle takes up oxygen from the blood and returns to it carbon dioxide, and also that the quantity of oxygen taken up is greater than the oxygen contained in the car- bon dioxide eliminated at the same time. The muscle, therefore, holds in some form of combination a part of the oxygen taken up while at rest. During activity the exchange of material in the muscle, and therewith the exchange of gas, is increased. The animal organism takes up considerably more oxygen in activity than when at rest, and eliminates also considerably more carbon dioxide (REGNAULT and REISET and others). The quantity of oxygen which leaves the body as carbon dioxide is, during activity, MUSCLE. 265 considerably larger than the quantity of oxygen taken up at the same time; and the venous muscle-blood is poorer in oxygen and richer in carbon dioxide during activity than during rest (LUDWIG and SCZELKOW and others). The exchange of gases in the muscles during activity is the reverse to when at rest, for the active muscle gives up a quantity of carbon dioxide which does not correspond to the quantity of oxygen taken up, but is considerably greater. It follows from this that in muscular activity not only oxidation takes place, but also splitting processes. This follows also from the fact that removed blood-free muscles, when placed in an atmos- phere devoid of oxygen, can labor for some time and also yield car- bon dioxide (HERMANN). During muscular inactivity a consumption of glycogen takes place. This is inferred from the observations of several investiga- tors that the quantity of glycogen is increased and its correspond- ing consumption reduced in those muscles whose chemical tonus is lowered either by cutting through the nerve or for other reasons (BERNARD, MCDONNEL, CHANDELON, and others. After cutting the nerve MANCHE obtained no doubtful results). In activity this consumption of glycogen is increased, and it has been positively proved by the researches of several investigators (NASSE, BRUCKE and WEISS, KULZ, MARCUSE, MANCHE) that the quantity of gly- cogen in the muscles in activity quickly and abundantly decreased. By investigating with the muscles in situ, especially on the levator labii superioris of a horse, CHAUVEAU and KAUFMANS have not only confirmed the above facts in regard to the exchange of gas during rest and activity, but they also found that the muscles remove sugar from the blood, and indeed considerably more during activity than when at rest. They found (calculating the amount found in 1 gramme of muscle per minute to 1 kilo per hour) that 1 kilo of muscle removes 2.186 grms. sugar from the blood per hour during rest, while it removes 8.416 grms. per hour in activity. QUINQUAUD has also observed a consumption of sugar from the blood during activity. On comparing the amount of carbon which the muscles remove from the blood as sugar with the amount they give off as carbon dioxide, GHAUVEAU and KAUFMANN found that in rest more carbon was taken up than given off, while during activity this condition was reversed. From these facts it is claimed 266 PHYSIOLOGICAL CHEMISTRY. by them that during rest glycogen is formed from the sugar, and also that in activity other bodies besides sugar are transformed in the muscles. The faintly alkaline or amphoteric reaction of the inactive muscles is changed during activity to an acid reaction (DuBois- KEYMOKD and others), and the acid reaction to a certain point increases with the work (HEIDENHAIN). The quickly-contracting pale muscles produce, according to GLEISS, more acid during ac- tivity than the more slowly-contracting red muscles. The acid reaction appearing during activity was formerly considered due to the formation of lactic acid, a view which has been contradicted by ASTASCHEWSKY, PFLUGER and WARREN", who found less lactic acid in the tetanized muscle than when at rest. According to the recent and more carefully conducted investigations of MARCUSE, which have since been confirmed by WERTHER, there is no doubt that free lactic acid is actually produced in the muscle during activity. According to WEYL and ZEITLER, the active muscle con- tains a larger amount of phosphoric acid (formed at least in part from the decomposition of lecithin) than the resting muscle; and the acid reaction of the active muscle may, therefore, in part be due to the acid phosphate. The amount of albumin in the removed muscles is, according to KANKE and NAWROCKI, decreased by work. The correctness of this statement is, however, disputed by other investigators. Also the older statements in regard to the nitrogenized extractive bodies of the muscle in rest and inactivity are uncertain. According to the recent researches of MOKARI, the total quantity of creatin and creatinin is increased by work ; and indeed by an excess of muscular activity, the amount of creatinin is especially augmented. The creatinin is formed essentially from the creatin. In excessive activity MOKARI also found xantho-creatinin in the muscle, and the quantity was one tenth of that of the creatinin. The quantity of xanthin bodies is, according to MOUARI, decreased under the influence of work. It seems to have been positively shown that the active muscle contains a smaller quantity of bodies soluble in water and a larger quantity of bodies soluble in alcohol than the resting muscle (HELMHOLTZ). An attempt has been made to solve the question relative to the MUSCLE. 267 behavior of the nitrogenized constituents of the muscle at rest and during activity, by determining the total quantity of nitrogen eliminated under these different conditions of the body. While we in the past agreed with the views of LIEBIG that the elimination of nitrogen by the urine was increased by muscular work, the researches of several experimenters, especially those of VOIT on dogs and VOIT and PETTENKOFER on man, have led to quite differ- ent results. They have shown that during work no increase or only a very insignificant increase in the elimination of nitrogen takes place. We must not conceal the fact that we have a series of experiments which show a significant increase in the exchange of proteids during or after work. We have as example the observations of FLINT * and PAVY on a pedestrian, v. WOLFF, v. FUNKE, KRETJZHAGE and KELLNER on a horse, and lately those of ARGUTINSKY on him- self, which show an undoubted increase in the elimination of nitrogen during or after work. The elimination of nitrogen is mainly dependent upon causes which will be spoken of later (Chapter XV), such as the quantity and composition of the food, the condition of the adipose tissue, the action of work on the respiratory mechanism, etc., etc., all of which can hardly have received sufficient consideration in the last- mentioned experiments. The strong proof which the very careful experiments of VOIT and of PETTENKOFER and VOIT furnish in support of this theory is hardly destroyed by these investigations; though we must admit that this question is still somewhat unsettled. Even if we consider that muscular work does not cause any increase in the elimination of nitrogen, which has been quite posi- tively proved, still we do not exclude the possibility of an increased exchange of proteids in the muscle. It is possible on account of the functional exchange action of the organs, of which RANKE has made a special study, that an increased exchange of proteid in the muscles may be compensated by a simultaneous decreased exchange 1 The results obtained by Prof. A. FLINT were derived from experi- ments made upon the pedestrian WESTON, who in 1870, in the American Insti- tute Building (New York), travelled a distance of 317£ miles in five days. The chemical part of the work was performed under the immediate supervision of Prof. R. O. DOREMUS. For fifteen days, five before, five during, and five after the walk, all ingesta and excreta were weighed or measured and analyzed. The experiments of Dr. PAVY were made in London in 1876.— TRANSLATOB. 268 PHYSIOLOGICAL CHEMISTRY. of proteid in other organs. But however this may be, the modern view is, notwithstanding, that the exchange of proteid in the muscle is not increased by activity. The investigations on the amount of fat in removed muscles during activity and at rest have not led to any definite results. The experiments of VOIT on a starving dog, and those of PETTE^- KOFEB and VOIT on a man, offer strong proofs to show that an increased decomposition of the fat takes place during activity. If the results from the investigations thus far made of the chemical processes going on in the active and inactive muscle were collected together, we should find the following characteristics for the active muscle. The active muscle takes up more oxygen and gives off more carbon dioxide than the inactive muscle; still the elimination of carbon dioxide is increased considerably more than the absorption of oxygen. In work a consumption of carbohydrates, glycogen, and sugar takes place. A consumption of sugar seems only to have been shown in muscle with blood circulation, while a consumption of glycogen also has been observed in removed muscle. Lactic acid is formed in activity, which is carried off by the blood and taken up and consumed by the liver. Acid-alkali phosphates also seem to be produced by work. In regard to the behavior of fats in removed muscles nothing is known with certainty, though an increase in the consumption of fat in the organism has been ob- served during activity. An increase in the nitrogenized extractive bodies of the creatin group seems also to occur. In regard to the albuminous bodies the views are contradictory; but an increased elimination of nitrogen as a direct consequence of muscular exer- tion has thus far not been positively proved. In close connection with the above-mentioned facts we have the question as to the origin of muscular activity so far as it has its origin in chemical processes. In the past the generally-accepted opinion was that of LIEBIG, that the source of muscular action con- sisted of a metabolism of the albuminous bodies; to-day another view prevails. PICK and WISLICENTJS climbed the Faulhorn and calculated the amount of mechanical force expended in the attempt. With this they compared the mechanical equivalent transformed in the same time, from the proteids, calculated from the nitrogen eliminated with the urine, and found that the work really performed MUSCLE. 269 was not by any means compensated by the consumption of proteid. It was therefore proved by this that albumin alone cannot be the source of muscular activity, and that this depends in great measure on the metabolism of non-nitrogenized substances. Many other ob- servations have led to the same result, especially the experiments of VOIT, of PETTENKOFER and VOIT, and of other investigators, whose experiments show that while the elimination of nitrogen remains un- changed, the elimination of carbon dioxide during work is very con- siderably increased. It is also generally considered as positively proved, that muscular work is produced, at least the greatest part, by the metabolism of non-nitrogenized substances. Nevertheless we are not warranted in the statement that muscular activity is produced entirely at the cost of the non-uitrogenized substance, and that the albuminous bodies are without importance as a source of force. Among the non-nitrogenized bodies we must accord to carbo- hydrates, glycogen, and sugar the first place as sources of force. The fact that the carbohydrates are consumed during exertion has been previously discussed, and their great importance as a source of activity is generally admitted. The most important is glycogen, — not only that which pre-exists in the muscles, but also that which is contained by the liver and conveyed by the blood to the muscles as sugar to be used in muscular work. If the consumption of carbohydrates in activity is positively proved, then the question arises whether the quantity of glycogen or carbohydrates which is at the disposition of the muscles is sufficient for the development of the living force produced within them. Though it is difficult to give a positive answer to this question, it still seems certain that, at least in some particular cases, the carbohydrates are not sufficient for the development of this force. According to statements whose correctness cannot be doubted, muscles free from glycogen can per- form work (BERNARD, LUCHSINGER). Even though no direct investigations on removed muscles have shown any increase in the consumption of fat during activity, still, from the above-mentioned experiments on the exchange of material, we conclude that the fats are also undoubtedly sources of muscu- lar force. In regard to the albuminous bodies we have no positive direct observations which tend to establish the importance attrib- 270 PHYSIOLOGICAL CHEMISTRY. uted to them by LIEBIG in muscular activity; but when we con- sider that from the albuminous bodies not only the fats proceed but also the carbohydrates, glycogen, it is difficult to see why the source of muscular force is not in part due to a metabolism of the proteids. Though it is probable that in work the carbohydrates in the first place, and then the fats, are consumed by an increased exchange, it is therefore not improbable that the albuminous bodies also take part, and that all three chief groups of organic foods or muscle-constituents undergo an increased exchange during activity Quantitative Composition of the Muscle. A large number of analyses have been made of the flesh of various animals for purely practical purposes, in order to determine the nutritive value of different varieties of meat; but we have no exact scientific analyses with sufficient regard to the quantity of different albuminous bodies and the remaining muscle-constituents; or these analyses being incomplete are of little value. To give the reader some idea of the variable composition of muscle-substance we give the following summary, chiefly obtained from K. B. HOFMANK'S book. The figures are parts per 1000. Muscles from Mammalia. Birds. Cold-blooded Solids ............................. 217-255 227-282 200 Water ........................ ... 745-783 717-773 800 Organic bodies ................... 208-245 217-263 180-190 Inorganic bodies ................... 9-10 10-19 10-20 Myosin .......................... 35-106 29.8-111 29.7-87 Stroma substance (DANILEWSKY) ---- 78-161 88.0-184 70.0-121 Alkali albuminate ................. 29-30 Creatin ........................... 2 3.4 2.3 Xanthin bodies .................... 0.4-0.7 0.7-1.3 Inosinic acid (barium salt) .......... 0.1 0.1-0.3 Prottc acid ........................ — 7.0 Taurin ..................... ....... 0.7 (horse) 1.1 Inosit ............................. 0.03 — — Glycogen ......................... 4-5 — 3-5 Lactic acid ....................... 0.4-0.7 — — Phosphoric acid ................... 3.4-4.8 Potash ........................... 3.0-4.0 Soda ............................. 0.4 Lime ......... ................... 0.2 Magnesia ......................... 0.4 Sodium chloride .................. 0.04-0.1 Iron oxide ........................ 0.03-0.1 MUSCLE. 271 In these tables, which have little value because of the variation in the composition of the muscles, we have no results as to the estimates of fat. Because of the variable quantity of fat in meat it is hardly possible to quote a positive average for this body. After most careful efforts to remove the fat from the muscles with- out chemical means, it has been found that a variable amount of inter-muscular fat, which does not really belong to the muscular tissue, always remains. The smallest quantity of fat in the mus- cles from lean oxen is, according to GROUVEN, 6.1 p. m., and accord- ing to PETERSEN, 7.6 p. m. This last observer also found habitually a smaller amount of fat, 7.6-8.6 p. m., in the fore quarter of oxen, and a greater amount, 30.1-34.6 p. m., in the hind quarter of the animal. A lower amount of fat has also been found in the muscles of wild animals. B. KOKIG and FARWICK found in the muscles of the extremities of the hare 10.7 p. m. fat, and 14.3 p. m. in the muscles of the partridge. The muscles of pigs and fattened ani- mals are, when all the appending fat is removed, very rich in fat, amounting to 40-90 p. m. The muscles of certain fishes also con- tain a large amount of fat. According to ALMEN, the flesh of the salmon, mackerel, and eel contain respectively 100, 164, and 329 p. m. fat. The quantity of WATER in the muscle is liable to considerable variation. The amount of fat has a special influence on the quan- tity of water, and we find, as a rule, that the flesh which is deficient in water is correspondingly rich in fat. The quantity of water does not depend alone upon the amount of fat, but upon many other circumstances, among which we must mention the age of the animal. In young animals the organs in general, and therefore also the muscles, are poorer in solids and richer in water. In man the amount of water decreases until mature age, but increases again towards old age. Work and rest also influence the amount of water, for the active muscle contains more water than the inactive. The uninterruptedly active heart should therefore be the muscle richest in water. That the amount of water may vary independ- ently of the amount of fat is strikingly shown by comparing the muscles of different species of animals. In cold-blooded animals the muscles generally have a greater amount of water, in birds a lower. The comparison of the flesh of cattle and fish shows very 272 PHYSIOLOGICAL CHEMISTRY. strikingly the different amounts of water (independent of the amount of fat) in the flesh of different animals. According to the analyses of ALMEN, the muscles of lean oxen contain 15 p. m. fat and 767 p. m. water; the flesh of the pike contains only 1.5 fat and 839 p. m. water. For certain purposes, as, for example, for the tests of the exchange of material, it is important to know the amount of nitrogen in the flesh. In general, it may be considered on an average as about 3.4$ of the fresh lean flesh (Vorr). According to NOWAK and HUPPERT this quantity may vary about 0.6$, and in more exact investigations it is therefore necessary to determine the nitrogen. Non-striated Muscles. The smooth muscles have a neutral or alkaline reaction (Du- BOIS-KEYMOND) when at rest. During activity they are acid, which is inferred from the observations of BERNSTEIN, who found that the nearly continually contracting sphincter muscle of the Anodonta is acid during life. The smooth muscles may also, according to HEIDENHAIN and KUHNE, pass into rigor mortis and thereby become acid. Because of this behavior it is believed that among the albuminous bodies of the smooth muscles there is also a substance forming myosin. A spontaneously coagulating plasma has not thus far been obtained, but it may be considered as the juice obtained by pressing the muscles of the Anodonta and which coagulates immediately at + 45° C. or within 24 hours at the ordinary temperature. Myosin has not been found in the smooth muscles. HEIDENHAIN and HELLWIG have obtained from the smooth muscles of a dog an albuminous body which coagulates at -j- 45°- 49° C. and which is analogous to musculin. The smooth muscles contain large amounts of alkali albuminates besides an albumin coagulating at -{- 75° C. HcemogloUn occurs in the smooth muscles of certain animals, but is absent in others. Creatin has been found by LEHMANN. Accord- ing to FREMY and VALENCIENNES, the muscles of the Cepha- lopods contain taurin besides creatinin (creatinf). Of the non- nitrogenized substances, glycogen and lactic acid have been found without doubt. The mineral constituents show the remarkable fact that the sodium combinations exceed the potassium combinations. CHAPTER X. BRAIN AND NERVES. ON account of the difficulty of making a mechanical separation and isolation of the different tissue elements of the nervous central organ and the nerves, we must resort to a few micro- chemical re- actions, chiefly to qualitative and quantitative investigations of the different parts of the brain, in order to study the different chemical composition of the cells and the nerve-tubes. The chemical investi- gation of this part is accompanied with the greatest difficulty; and although our knowledge of the chemical composition of the brain and nerves has been somewhat extended by the investigations of modern times, still we must admit that this chapter is yet to-day one of the most obscure and complicated in physiological chemistry. Albuminous bodies of different kinds have been shown to be chemical constituents of the brain and nerves. A part of these are insoluble in water and dilute neutral-salt solutions, and part are soluble therein. Among the latter we find albumin and globulin. Nudeoalbumin, which is often considered as an alkali albnminate, also occurs. It seems unquestionable that the albuminous bodies belong chiefly to the gray substance of the brain and to the axis- cylinders. The same remarks apply to nuclein which v. JAKSCH found in large quantities in the nerve subtances. Neurokeratin (see page 35), which was first detected by KiJHNE,and which partly forms the neuroglia, and which as a double sheath envelops the outside of the nerve medulla under SCHWANN'S sheath and the inner axis-cylinders, chiefly occurs in the white substance (KuHJjE and CHITTENDEN, BAUMSTARK). The phosphorized substance protagon must be considered as one of the chief constituents, perhaps the only constituent (BAUM- STARK), of the white substance. This last-mentioned substance 273 274 PHYSIOLOGICAL CHEMISTRY. yields easily, as decomposition products, lecithin, fatty acids, and a nitrogenized substance, cerebrin; this last probably does not occur preformed in the brain, but is more likely a product of transforma- tion (BAUMSTABK). That lecithin also is pre-existent in the brain and nerves can hardly be doubted. The investigations thus far made have not shown decidedly whether ifc is more abundant in the gray or white substance. Fatty acids and neutral fats may be prepared from the brain and nerves; but as these may be readily derived from a decomposition of lecithin and protagon, which exist in the fatty tissue between the nerve-tubes, it is difficult to decide what part the fatty acids and neutral fats play as constituents of the real nerve-substance. Cholesterin is also found in the brain and nerves, a part free and a part in chemical combination of which we know nothing about (BAUMSTARK). Cholesterin seems to occur in greater abundance in the white substance. Besides these substances the nerve tissue, especially the white substance, contains doubtless a number of other constituents not well known, and among which are several containing phosphorus. THUDICHUM asserted that he had isolated a number of phosphorized substances from the brain which he divided into three principal groups: kepalines, myelines, and lecithines. But thus far this assertion has not been confirmed by other investigators. By allowing water to act on the contents of the medulla, round, or long double-contoured drops or fibres, not unlike double-con- toured nerves, are formed. This remarkable formation, which can also be seen in the medulla of the dead nerve, has been called " myeline forms" and they were formerly considered as produced from a special body, " myeline." Myeline forms may, however, be obtained from other bodies, such as protagon, lecithin, .fat, and im- pure cholesterin, and they depend on a decomposition of the con- stituents of the medulla, chiefly the protagon. The extractive bodies seem to be almost the same as in the mus- cles. We find : creatin, which may, however, be absent (BAUM- STARK), xanthin bodies, inosit, lactic acid (also fermentation lactic acid), uric acid, jecorin (according to BALDI, in the human brain), and neuridin, discovered by BRIEGER and which is most interest- ing because of its appearance in the putrefaction of animal tissues. Under pathological conditions leucin and urea have been found in BRAIN AND NERVES. 275 the brain. The latter is also a physiological constituent of the brain of cartilaginous fishes. Of the above-mentioned constituents of the nerve-substance, protagon and its decomposition product, cerebrin, must be specially described. Protagon. This body, which was discovered by LIEBREICH, is a nitrogenized and phosphorized substance whose elementary compo- sition, according to GAMGEE and BLANKENHOKN", is C 66.39, H 10.69, N 2.39, and P 1.068 per cent, and whose empirical formula is CieoHsogNgPOsg. On boiling with baryta-water, protagon yields the decomposition products of lecithin, namely, fatty acids, glycero- phosphoric acid, and cholin (neurin?), and also cerebrin. On boil- ing with dilute mineral acids, it yields among other products a substance which is laevo-gyrate, reducible, and fermentable. Protagon appears, when dry, as a white loose powder. It dis- solves in alcohol of 85 vols. per cent at -f- 45° C., but separates on cooling as a snow-white, flaky precipitate, consisting of balls or groups of fine crystalline needles. It decomposes on heating even below 100° C. It is hardly soluble in cold alcohol or ether, but dissolves on warming. It swells in little water, decomposes partly, and gives myaline forms. With more water it swells to a gelatinous or pasty mass, which with much water yields an opalescent liquid. On fusing with saltpetre and soda, alkali phosphates are obtained. Protagon is prepared in the following way : An ox-brain as fresh as possible, with the blood and membranes carefully removed, is ground fine and then extracted for several hours with alcohol of 85 vols. per cent at -f- 45° C., filtered at the same temperature, and the residue extracted with warm alcohol until the filtrate does not yield a precipitate at 0° C. The several alcoholic extracts are cooled to 0° C. and the precipitates united and completely extracted with cold ether, which dissolves the cholesterin and lecithin-like bodies. The residue is now strongly pressed between filter-paper and allowed to dry over sulphuric acid or phosphoric anhydride. It is now pulverized, digested with alcohol at -}- 45° C., filtered and slowly cooled to 0° C. The crystals which separate may be purified when necessary by recrystallization. The same steps are taken when we wish to detect the presence of protagon. Cerebrin. Under this name W. MULLER first described a ni- trogenized substance, free from phosphorus, which he obtained by 276 PHYSIOLOGICAL CHEMISTRY. extracting a brain-mass which had been previously boiled with baryta- water, with boiling alcohol. Following a method essentially the same, but differing somewhat, GEOGHEGAN prepared from the brain a cerebrin with the same properties as MULLER'S, but con- taining less nitrogen. According to GAMGEE, this cerebrin of GEOGHEGAN is a mixture of the cerebrin produced by the decom- position of the protagon and a substance called by GAMGEE " psendo- cerebrin." Furthermore, THUDICHUM has collected together under the name "cerebrine" several nitrogenized bodies free from phosphorus, such as MULLER'S cerebrin and the newer bodies phrenosine and kerasene. Lastly, PAKCUS has endeavored to prove that the cerebrin described by both MULLEE and GEOGHEGAN" is a mixture of three bodies, "cerebrin," " homocerebrin," and " en- cephalin." From the above statements it is seen that the composition of the cerebrins is still unsettled, so that we cannot admit the numerical results of any inves- tigator as authoritative, nor can we even take an average as correct, and we therefore give below a summary of the results thus far obtained : C H N Mailer's cerebrin 68.45 11.20 4.50 per cent Geoghegan's cerebrin 68.74 10.91 1.44 Parcus's cerebrin 69.08 11.47 2.13 Parcus's homocerebrin 70.06 11.60 2.23 Parcus's encephalin 68.40 11.60 3.09 Gamgee's pseudocerebrin 68.89 11.87 1.83 Thudichum's kerasene 68.90 11.36 1.74 As GEOGHEGAN'S cerebrin has the lowest amount of nitrogen, and as the analyses of this investigator agree well with each other, it is difficult to under- stand how this cerebrin can be, as PARCUS' claims, a mixture of bodies richer in nitrogen which were prepared by this last-named investigator. On the other hand, PAKCUS has found a constant composition for the cerebrin irrespective whether it was recrystallized two, five, or eight times, and it i& therefore quite as unjustifiable to question his results. Future researches are required to elucidate this question. GAMGEE'S pseudocerebriu and THUDI- CHUM'S kerasene have, with the exception of the amount of hydrogen, so sim- ilar a composition that they may be considered as identical. The products of decomposition of the cerebrins possess a certain interest. By the action of concentrated sulphuric acid GEOGHEGAN obtained a lasvo- gyrate, reducible substance, which is not sugar but an acid. As chief product a substance is obtained which he called "cetylid," C^H^O5 , and which on fusing with caustic potash yields marsh-gas, hydrogen, and palmitic acid. According to him, this cetylid is probably a derivative of cetyl-alcohol. Of special interest is the proof, as first shown by THUDICHUM and lately substantiated by THIERFELDER, that on heating the so- called cerebrin with dilute sulphuric acid a glucose is split off. BRAIN AND NERVES. 277 This glucose is, as THIERFELDEK has proved, identical with GALAC- TOSE. If we put aside the above differences in the composition and certain somewhat differing statements in regard to the qualitative reactions of cerebrin, there are still a few general reactions for all cerebrin preparations which may be employed in their detection. From these lately-described properties it has been claimed that, besides in the brain and nerves, cerebriu occurs in the pus-cells, and in the electric organ of the ray. GEOGHEGAN has also found it in a cancer of the liver. Cerebrin, as generally described, is in the dry state a loose, purely while, odorless and tasteless powder. On heating it becomes brown at about 80° C., puffs up, on continuing the heat melts and gradually decomposes. It is insoluble in water, cold alcohol, or ether, also in dilute caustic alkali or baryta-water. It swells in boiling water, forming a pasty mass. It dissolves in boiling alcohol (also ether), but on cooling a flocculent precipitate separates, which on microscopic examination is found to consist of a series of balls or grains. Cerebrin is chiefly characterized by these properties, and by its yielding a reducible substance on boiling with dilute mineral acids. The PARCUS'S cerebrin differs from the ordinary in the follow- ing particulars : it is not soluble in boiling ether ; on warming it melts without decomposing (which takes place at 145°-160° C.); it gives a light yellow solution with concentrated sulphuric acid, and it swells very little in cold water. The HOMocEREBRiN and ENCEPHALIN of PARCUS remain iu the mother liquor after the precipitation of the impure cerebriu from the alcohol. These bodies have a tendency to separate as gelatinous mass. Homocerebrin, which, according to PARCUS, is homologous to cerebrin, is similar to it, but dissolves more easily in warm alcohol and also in^ warm ether. It may also be obtained as extremely fine needles. Encephalin is claimed by PARCUS to be a product of the metamorphosis of cerebriu. In the perfectly pure state it crystallizes in small plates. It swells in warm water, forming a pasty mass. Like the cerebrin and the homocerebin, it yields a reducible substance on boiling with dilute acids. GAMGEE'S PSEUDOCEREBRIN, which has thus far not been closely studied, is obtained as a by-product in the recrystallization of protagon. The cerebrins are generally prepared according to MULLER'S method. The brain is first stirred with baryta-water until it ap- pears like thin milk, and then it is boiled. The insoluble parts 278 PHYSIOLOGICAL CHEMISTRY. are removed, pressed, and repeatedly boiled with alcohol, which is filtered while boiling hot. The impure cerebrin which separates on cooling is freed from cholesterin and fat by means of ether, and then purified by repeated solution in warm alcohol. According to PARCUS, this repeated solution in alcohol is continued until no gelatinous separation of homocerebrin or encephalin takes place. According to GEOGHEGAN'S method, the brain is first extracted with cold alcohol and ether and then boiled with alcohol. The precipitate which separates on the cooling of the alcoholic filtrate is treated with ether and then boiled with baryta-water. The in- soluble residue is purified by repeated solution in boiling alcohol. The cerebrin may also be obtained from other organs by em- ploying the above methods. The quantitative estimation, when such is desired, may be performed in the same way. Neuridin, C5Hi4N2 , is a non-poisonous diamin discovered byBniEGER, and which was obtained by him in the putrefaction of meat and gelatine. It also occurs under physiological conditions in the brain, and as traces in the yolk of the egg. Neuridin dissolves in water, and yields on boiling with alkalies a mixture of dimethylamin and trimethylamin. It dissolves with difficulty in amyl-alcohol. It is insoluble in ether or absolute alcohol. In the free state neuridin has a peculiar odor, suggesting semen. With hydrochloric acid it gives a combi- nation crystallizing in long needles. With platinic chloride or gold chloride it gives crystallizable double combinations which are valuable in its prepara- tion and detection. The so-called CORPUSCULA AMYLACEA, which occur on the upper surface of the brain and in the pituitary gland, are colored more or less pure violet by iodine and more blue by sulphuric acid and iodine. They consist, perhaps, of the same substance as certain prostatic calculi, but they have not been, closely investigated. Quantitative Composition of the Brain. The quantity of water is greater in the gray than in the white substance, and greater in new- born or young individuals than in grown ones. The brain of the foetus contains 879-926 p. m. water. According to the observations of WEISBACH, the amount of water in the several parts of the brain (and in the medulla) varies at different ages. The following fig- ures are in 1000 parts ; A for men and B for women. White substance of the brain Gray ditto Gyri Cerebellum Pons varoli Medulla oblongata . 20-30 Years. A. #? 30-50 Years. 50-70 Years. 70-94 Years, 695.6 833.6 784.7 788.3 734.6 744.3 682.9 826.2 792.0 794.9 740.3 740.7 6831 83fi.l 795.9 778.7 7255 732.5 703.1 830.6 772.9 789.0 722.0 729.8 A. 701.9 838.0 796.1 787.9 720.1 722.4 B. 689.6 838.4 796.9 784.5 714.0 730.6 726.1 847.8 802.3 803.4 727.4 736.2 722.0 889.5 801.7 797.9 724.4 736.7 BRAIN AND NERVES. 279 Quantitative analyses of the brain have also been made by PETROWSKY on an ox-brain and by BAUMSTARK on the brain of a horse. In the analyses of PETROWSKY the protagon has not been considered, and all organic, phosphorized substances were calcu- lated as lecithin. On these grounds these analyses are not of much value from a certain standpoint. In BAUMSTARK'S analyses the gray and the white substance could not be sufficiently separated, and these analyses, on this account, show partly an excess of white and partly an excess of gray substance ; nearly one half of the organic -bodies, chiefly consisting of bodies soluble in ether, could not be exactly analyzed. Neither do these analyses give sufficient explanation of the quantitative composition of the brain. The analyses made up to the present time give, as above stated, an equal division of the organic constituents in the gray and white substance. In the analyses of PETROWSKY the quantity of albumin and gelatine-forming substances in the gray matter was somewhat more than one half, and in the white about one quarter of the solid organic substances. The quantity of cholesterin in the white was about one half, and in the gray substance about one fifth, of the solid bodies. A greater quantity of soluble salts and extractive bodies was found in the gray substance than in the white (BAUM- STARK). The following analyses of BAUMSTARK give the most im- portant known constituents of the brain calculated in 1000 parts of the fresh, moist brain. A represents chiefly the white, and B chiefly the gray, substance. A. B. Water 605.35 769.97 Solids 304.65 230.03 Protagon 25.11 10.80 Insoluble albumin and connective tissue 50.02 60.79 Cholesterin, free 18.19 6.30 combined 26.96 17.51 Nuclein 2.94 1.99 Neurokeratin 18.93 10.43 Mineral bodies 5.23 5. 62 The remainder of the solids probably consists chiefly of lecithin and other phosphorized bodies. Of the total amount of phos- phorus, 15-20 p. m. belongs to the nuclein, 50-60 p. m. to the protagon, 150-160 p. m. to the ash, and 770 p. m. to the lecithin and the other phosphorized organic substances. 280 PHYSIOLOGICAL CHEMISTRY. The quantity of neurokeratin in the nerves and in the different parts of the brain has been carefully determined by KUHNE and CHITTENDEKT. They found 3.16 p. m. in the plexus brachialis, 3.12 p. m. in the edge of the cerebellum, 22.434 p. m. in the white substance of the large brain, 25.72-29.02 p. m. in the white sub- stance of the corpus callosum, and 3.27 p. m. in the gray substance of the edge of the large brain (when free as possible from white substance). The quantity of mineral constituents in the brain amounts to 2.95-7.08 p. m., according to GEOGHEGAN. He found in 1000 parts of the fresh, moist brain 0.43-1.32 01, 0.956-2.016 P04, 0.244-0.796 C03, 0.102-0.220 S04 , 0.01-0.098 Fe2(P04)2, 0.005-0.022 Ca, 0.016- 0.072 Mg, 0.58 to 1.778 K, 0.450-1.114 Na. The gray substance yields an alkaline ash, the white an acid ash. Appendix. The Tissue and Fluids of the Eye. The retina contains in all 816-880 p. m. water, 72-102 p. m. proteid bodies — myosin, albumin, and mucin (?), 9-32 p. m. lecithin, and 2.7-10.6 p. m. salts (HOPPE-SEYLER and KAHN). The mineral bodies consist of 420 p. m. NagHPO^ and 350 p. m. NaCl. Those bodies which form the different segments of the rods have not been closely studied, and the greatest interest is therefore connected with the coloring matters of the retina. Visual purple, also called rhodopsin, erythropsin, or VISUAL RED, is the pigment of the rods. BOLL observed in 1876 that the layer of rods in the retina during life had a purplish-red color which was bleached by the action of light. KUHNE showed later that this red color might remain for a long time after the death of the ani- mal if the eye was protected from daylight or investigated by a sodium light. By these conditions it was also possible to isolate and closely study this substance. Visual red (BOLL) or visual purple (KUHNE) has become known mainly by the investigations of KUHNE. The pigment occurs chiefly in the rods and only in their outer parts. In animals whose retina has no rods the visual purple is absent, and is also necessarily BRAIN AND NERVES. 281 absent in the macula lutea. In a variety of bat (rliinoloplius hip- posideros), in hens, pigeons, and new-born rabbits, no visual purple has been found in the rods. A solution of visual purple in water which contains 2-5# crystallized bile, the best solvent for it, is purple-red in color, quite clear, and not fluorescent. On evaporating this solution in vacua, we obtain a residue similar to ammonium carminate which contains violet or black grains. If the above solution is dialyzed with water, the bile is diffused and the visual purple separates as a violet mass. Under all circumstances, even when still in the retina, the visual purple is quickly bleached by direct sunlight, and with diffused light with a rapidity corresponding to the intensity of the light. It passes from red and orange to yellow. Ked light bleaches the visual purple slowly; the ultra-red light does not bleach it at all. A solution of visual purple shows no special absorption-bands, but only a general absorption which extends from the red side, beginning at Z>, to the line G. The strongest absorption is found at K Visual purple when heated to 52°-53° C. is destroyed after several hours, and almost instantly when heated to +76° C. It is also destroyed by alkalies, acids, alcohol, ether, and chloroform. On the contrary, it resists the action of ammonia or alum solution. As the visual purple is easily destroyed by light, it must there- fore also be regenerated during life. KUHISTE has also found that the retina of the eye of the frog becomes bleached when exposed for a long time to strong sunlight, and that its color gradually returns when the animal is placed in the dark. This regeneration of the visual purple is a function of the living cells in the layer of the pigment epithelium of the retina. This may be inferred from the fact that a detached piece of the retina which has been bleached by light may have its visual purple restored if the detached piece of the retina be carefully laid on the chorioi'dea having layers of the pigment epithelium attached. The regeneration has, it seems, nothing to do with the dark pigment, the melanin or fuscin, in the epithelium cells. A partial regeneration seems, according to KUHNE, to be possible in the completely-removed retina. On ac- count of this property of the visual purple of being bleached by light during life, we may, as KUHNE has shown, under special con- ditions and by observing special precautions, obtain after death by 282 PHYSIOLOGICAL CHEMISTRY. the action of intense light or more continuous light the picture of bright objects, such as windows and the like, so-called -optograms. The physiological importance of visual purple is unknown. It follows that the visual purple is not essential to sight, since it is ab- sent in certain animals and also in the cones. HOLMGREK has further shown that in the eye of a frog or rabbit, whose retina has been previously bleached by continuous light, a now of nega- tive electricity is continually observed in the optic nerve when the eye is exposed to the action of light. Visual purple must always be prepared exclusively in a sodium light. It is extracted from the net membrane by means of a watery solution of crystallized bile. The filtered solution is evapo- rated in vacuo or dialyzed until the visual purple is separated. The pigments of the cones. In the inner segments of the cones of birds, reptiles, and fishes a small fat-globule of varying color is found. KUHNE has isolated from this fat a green, a yellow, and a red pigment called respectively chlorophan, xanthopJtan, and rhodophan. The dark pigment of the epithelium cells of the net membrane, which was formerly called melanin, but since named fuscin by KUHNE, dissolves iu concentrated caustic alkalies or concentrated sulphuric acid on warming, but, like melanine in general (see Chapter XIII), has been little studied. The pig- ment occurring in the pigment-cells of the chorioi'dea seems to be identical with the fuscin of the retina. The vitreous humor is often considered as a variety of gelatinous tissue. It contains albumin and, as C. TH. MORNER has shown, a mucoid (see page 234). Among the extractives we find a little urea — according to PICARD, 5. p. m., and according to ROHMABW, 0.64 p. m. The reaction of the vitreous humor is alkaline, and the quantity of solids amounts to about 11 p. m. The quantity of mineral bodies is about 9 p. m., and the albuminous bodies 0.7 p. m. In regard to the aqueous humor see page 126. The crystalline lens. That substance which forms the capsule of the lens has not had its chemical nature closely investigated, but gives a reducible body when boiled with dilute acids (0. TH. MORNER). The real substance of the lens, the lens-fibres, contains as chief constituent a globulin which is nearly related to vitellin arid to which BERZELIUS gave the name crystallin. This globulin may be partially precipitated from a watery extract of the lens by means of C02 , and it may be completely separated by satu- rating with MgS04. The statements as to the occurrence of an albumin besides the globulin in the lens are disputed; that at least BRAIN AND NERVES. 283 in the lens of cattle an albumin occurs is nevertheless easy to de- monstrate. A. BECHAMP distinguishes the two following albuminous bodies in the watery extract of the crystalline lens. Phacozymase, which coagulates at -f 55° C., contains a diastatic enzyme and has a specific rotary power of (a); = — 41°, and the crystalbumin, with a specific rotary power of (a#"= —80.3°. BECHAMP extracted from the residue of the lens, which was insoluble in water, by means of hydrocoloric acid, an albuminous body which had a specific rotary power of (a)j = — 80.2° which he called crystal- fibrin. The lens does not seem to contain any albuminous body which coagulates spontaneously like fibrinogen. That cloudiness which appears after death depends, according to KUHNE, upon the un- equal changing of the concentration of the contents of the lens- tubes. This change is produced by the altered ratio of diffusion. A cloudiness of the lens may also be produced in life by a rapid removal of water, as, for example, when a frog is plunged into a salt or sugar solution (KtnsrDE). The appearance of cloudiness in diabetes has been attributed by some to the removal of water. The views on this subject, however, do not accord. The average results of four analyses made by LAPTSCHINSKY of the lens of oxen are here given, calculated in parts per 1000: Albuminous bodies 349. 3 Lecithin 2.3 Cholesterin 2.2 Fat 2.9 Soluble salts 5.3 Insoluble salts &8 In cataract the amount of albumin is diminished and the amount of cholesterin increased. The corneal tissue has been previously treated of (page 237). The sclerotica has not been closely investigated, and the chlorioidea is chiefly of interest because of the coloring matter, melanine, it contains (see Chap. XIII). TEARS consist of a water-clear, alkaline fluid of a saltish taste. According to the analyses of LEKCH, they contain 982 p. m. water, 18 p. m. solids, with 5 p. m. albumin and 13 p. m. NaCl. 284 PHYSIOLOGICAL CHEMISTRY. The Fluids of the Inner Ear. The perilymph and endolymph are alkaline fluids which, besides salts, contain — in the same amounts as in transudations — traces of albumin, and in certain animals (torsk) also mucin. The quantity of mucin is greater in the perilymph than in the endolymph. Otoliths contain 745-795 p. m. inorganic substance which con- sists chiefly of crystallized calcium carbonate. The organic sub- stance is very like mucin. CHAPTER XL ORGANS OF GENERATION". (a) Male Generative Secretions. THE testes have been little investigated chemically. We find in the testes of animals albuminous bodies of different kinds, serum albumin, alkali albuminate (?), and an albuminous body related to ROVIDAS' hyaline substance, also leucin, tyrosin, creatin, xanthin bodies, cholesterin, lecithin, inosit, and/#£. In regard to the occur- rence of glycogen the statements are somewhat contradictory. DARESTE found in the testes of birds granules similar to starch, which were colored blue with difficulty by iodine. The semen as ejected is a white or whitish -yellow, viscous, sticky fluid of a milky appearance, with whitish, non- transparent lumps. The milky appearance is due to semen-fibres. Semen is heavier than water, contains albumin, has a neutral or faintly- alkaline reaction, and a peculiar specific odor. The semen of bulls has an acid reaction. Soon after ejection semen becomes gelati- nous, as if it were coagulated, but afterwards becomes more fluid. When diluted with water white flakes or shreds separate (HENLE'S fibrin). According to the analyses of VAUQUELIN", the human semen contains 900 p. m. water and 100 p. m. solids, with 60 p. m. organic and 40 p. m. inorganic substance, of which 30 p. m. is cal- cium phosphate. Among the albuminous bodies POSNER claims that propeptone occurs even in the absence of the semen-fibres. The semen in the vas deferens differs chiefly from the ejected in that it is without the peculiar odor. This last depends on the admixture with the secretion of the prostate. This secretion, ac- cording to IVERSEK, has a milky appearance and ordinarily an alkaline reaction, very rarely a neutral one, contains small amounts 285 286 PHYSIOLOGICAL CHEMISTRY. of proteids and mineral bodies, especially NaCl. Besides these it contains a crystalline combination of phosphoric acid with a base, C2H6N. This combination has been called BOTTCHER'S spermine crystals, and it is claimed that the specific odor of the semen is due to a partial decomposition of these crystals. The crystals which appear on slowly-evaporating the semen, and which are also observed in anatomical preparations kept in alcohol, and in desiccated egg-albumin, are identical, according to Sen REINER, with CiTARCOT's crystals found in the blood, and in the lymphatic glands in leucaemia. They are, according to SCHREI- NER, a combination of phosphoric acid with a base, C2H6N, which he discovered, and this base is, according to LADENBURG and ABEL, probably ethylenimin. The semen-fibres (spermatozoa) show a great resistance to chemical reagents in general. They do not dissolve completely in concentrated sulphuric acid, nitric acid, acetic acid, nor in boiling- hot soda solutions. They are dissolved by a boiling-hot caustic- potash solution. They resist putrefaction, and after drying they may be obtained again in their original form by moistening them with a \ common-salt solution. By careful heating and burning to an ash the shape of the spermatozoa maybe seen in the ash. The quantity of ash is about 50 p. m. and consists mainly (f ) of potas- sium phosphate. The spermatozoa show well-known movements, but the cause of this is not known. This movement may continue for a very long time, as under some conditions it may be observed for several days in the body after death, and in the secretion of the uterus longer than a week. Acid liquids stop these movements immediately; they are also destroyed by strong alkalies, especially ammoniacal liquids, also by distilled water, alcohol, ether, etc.- The movements continue for a longer time in faintly-alkaline liquids, especially in alkaline animal secretions, and also in properly-diluted neutral salt-solutions. According to the investigations of MIESCHER, there are lecithin and nuclein but no cerebrin in the SPERMATOZOA of BULLS. The head of the spermatozoa contains nuclein, which forms probably the outer part of the head: albumin, which forms the contents of the head; and lastly a substance rich in sulphur which has not been studied. The tail dissolves in gastric juice after continuous ORGANS OF GENERATION. 287 digestion, find seems to consist of albuminous bodies, or bodies closely related, which show a variable resistance towards pepsin- hydrochloric acid. The semen of bulls does not contain any adenin, but the other three xanthin bodies treated of on pages 49-51 (KossEL and SCHINDLER) are present. The SPERMATOZOA of the RHINE SALMON show, according to MIESCHER, a great resistance. With caustic-potash and soda solu- tions they give a cloudy, gelatinous mass which is precipitated as shreds by acids ; but these shreds do not dissolve in an excess of the acid. They are strongly attacked by a 10-15$ solution of NaCl or NaN03, and the semen is converted by such a solution into a stiff gelatin. The head is attacked, but not the tail or the middle part. This last-mentioned part, like the tail, contains albu- min, which is dissolved by hydrochloric acid of 1 p. m., but not in Nad. MIESCHER also found lecithin, fat, cliolesterin, guanin, and sarkin in relatively large amounts in the salmon-semen. The organic constituent occurring in the largest amount in the salmon- semen is, according to MIESCHER, a combination of nuclein with the base protamin, CgHaoNgO^OH, which is soluble in water but insoluble in alcohol or ether. According to MIESCHER and PICCARD, the spermatozoa of sal- mon contain 487 p. m. nuclein-protamin (268 p. m. protamin), 103 p. m. albuminous bodies, 75 p. m. lecithin, 60-80 p. m. guanin and sarkin, 22 p. m. cholesterin, and 45 p. m. fat. KOSSEL and SCHINDLER found no guanin, but xantJiin and large amounts of adenin and hypoxanthin, in the SEMEN of the CARP. Spermatin is a name which has been given to a constituent similar to alkali album inate, but it has not been closely studied. Prostatic concrements are of too kinds. One is very small, generally oval in shape with concentric layers. In young but not in older persons they are colored blue by iodine (!VERSEN). According to PAULICKY, they yield sugar when warmed with dilute sulphuric acid ; but this statement has not been substantiated by IVERSEN. The other kind is larger, sometimes the size of the head of a pin, and consisting chiefly of calcium phosphate (about 700 p. m.) with only a very small amount, about 160 p. m., organic substance. (b) Female Generative Organs. The stroma of the ovaries are of little interest from a physio- logico-chemical standpoint, and the most important constituent of the ovaries, the Graaffian vesicles with the ovum, have thus far not 288 PHYSIOLOGICAL CHEMISTRY. been the subject of a careful chemical investigation. The fluid in the vesicles (of the cow) do not contain, as has been stated, the peculiar bodies, paralbumin or metalbumin, which are found in certain pathological ovarial fluids, but seems to be a serous liquid. The corpora lutea are colored yellow by an amorphous coloring- mat- ter called lutein. Besides this another coloring-matter sometimes occurs which is not soluble in alkali ; it is crystalline, but not iden- tical with bilirubin or haematoidin ; but it may be identified as a lutein by its spectroscopic behavior (PICCOLO and LIEBEK, KtiH^E and EWALD). The cysts often occurring in the ovaries are of special patho- logical interest, and these may have essentially different contents, depending upon their variety and origin. The serous cysts (HYDROPS FOLLICULORUM GRAAFII), which are formed by a dilation of the Graafian vesicles, contain a serous liquid which has a specific gravity of 1.005-1.022. A specific gravity of 1.020 is less frequent. Generally the specific gravity is lower, 1.005-1.014, with 10-40 p. m. solids. As far as is known, the contents of these cysts do not essentially differ from other serous liquids. The proliferous cysts (MYXOID CYSTS, COLLOID CYSTS). The contents of these cysts have variable properties. We sometimes find in small cysts a semi-solid, transparent, or somewhat cloudy or opalescent mass which appears like solidified glue or quivering jelly and which has been called colloid because of its physical properties. In other cases the cysts contain a thick, tough mass which can be drawn out into long threads, and as this mass in the different cysts is more or less diluted with serous liquids their contents may have a variable consistency. The color of the contents is also variable. In certain cases they are bluish white, opalescent, and in others yellow, yellowish brown, or yellowish with a shade of green. They are often colored more or less choc- olate-brown or red-brownp due to the decomposed blood-coloring matters. The reaction is alkaline or nearly neutral. The specific gravity, which may vary considerably, is generally 1.015-1.030, but may in few cases be 1.005-1.010 or 1.050-1.055. The amount of solids is variable. In rare cases they amount to only 10-20 p. m. ; ORGANS OF GENERATION. 289 ordinarily they vary between 50-70-100 p. m. In a few cases 150-200 p. m. solids have been found. As form-elements we find red and colorless blood-corpuscles, granular cells, partly fat-degenerated epithelium and partly large so-called GLUGE'S corpuscles, fine granular masses, epithelium-cells, cliolesterin-crystals, and colloid corpuscles — large, circular, refractive formations. Though the contents of the proliferous cyst may have a variable composition, still it may be characterized in typical cases by its slimy or ropy consistency; by its grayish-yellow, chocolate-brown, sometimes whitish-gray color, and by its relatively high specific gravity, 1.015-1.025. Such a liquid does not ordinarily show a spon- taneous fibrin-coagulation. We consider colloid, metalbumin and paralburnin as character- istic constituents of these liquids. Colloid. This name does not designate any particular chemical substance, but is given to the contents of tumors with certain physical properties similar to gelatinous glue. Colloid is found as a diseased product in several organs. Colloid is a gelatinous mass, insoluble in water and acetic acid; it is dissolved by alkalies and gives a liquid which is not precipi- tated by acetic acid or by acetic acid and potassium ferrocyanide. Sometimes a colloid is found which, when treated with a very dilute alkali, gives a solution similar to a mucin solution. On boiling with acids colloid gives a reducible substance. It is related to mucin, and it is considered by certain investigators as a trans- formed mucin. A colloid found by WURTZ in the lungs contains C 48.09, H 7.47, N 7.00, and 0 37.44^. Colloids of different origin seem to have an unequal composition. Metalbumin. This name SCHERER gave to a proteid substance found by him in an ovarial fluid. The metalbumin was considered by SCHERER to be an albuminous body, but it belongs to the mucin group and it is therefore called by the author pseudomucin. Pseudomucin. This body, which, like mucin, gives a reducible substance when boiled with acids, is a mucoid of the following composition: C 49.75, H6.98, N 10.28, 81.25, 0 31.74# (AUTHOR). With water pseudomucin gives a slimy, ropy solution, and it is this substance which gives the fluid contents of the ovarial cysts their 290 PHYSIOLOGICAL CHEMISTRY. typical ropy property. Its solutions do not coagulate on boiling but only become milky-opalescent. Unlike mucin solutions, pseudomucin solutions are not precipitated by acetic acid. With alcohol they give a coarse flocculent or thready precipitate which only dissolves in water or alcohol after having been kept in these liquids for a long time. Paralbumin is another substance discovered by SCHERER and which occurs in ovarial liquids and also in ascites fluids with the simultaneous presence of ovarial cysts and rupture of the same. It is therefore only a mixture of pseudomucin with variable amounts of albumin, and the reactions of paralbumin are correspondingly variable. The detection of metalbuinin and paralbumin is naturally con- nected with the detection of pseudomucin. A typical ovarial fluid containing pseudomucin is, as a rule, sufficiently characterized by its physical properties, and a special chemical investiga- tion is only necessary in cases where a serous fluid contains very small amounts of pseudomucin. We proceed in the following way: The albumin is removed by heating to boiling with the addition of acetic acid; the filtrate is strongly concentrated and precipitated by alcohol. The precipitate is carefully washed with alcohol and then dissolved in water. A part of this solution is digested with saliva at the temperature of the body and then tested for glu- cose (derived from glycogen or dextrin). If glycogen is present, it will be converted into glucose by the saliva; precipitate again with alcohol and then proceed as in the absence of glycogen. In this last-mentioned case, first add acetic acid to the solution of the alcohol precipitate in water so as to precipitate any existing mucin. The precipitate produced is filtered, the filtrate treated with 2# HC1 and warmed on "the water-bath until the liquid is deep brown in color. In the presence of pseudomucin this solution gives TROMMER'S test. The other proteid bodies which have been found in cystic fluids are serum-globulin and serum-albumin) peptone (?), mucin, and mucin-peptone (?). Fibrin only occurs in exceptional cases. The quantity of mineral bodies on an average amount to about 10 p. m. The amount of extractive bodies (cholesterin and urea) and fat is ordinarily 2-4 p. m. The remaining solids, which con- stitute the chief mass, are albuminous bodies and pseudomucin. The intraligamentary, papillary cysts contain a yellow, yellowish- ORGANS OF GENERATION. 291 green, or brownish-green liquid which either contains no pseudo- mucin or — with the simultaneous degeneration of colloid — very little. The specific gravity is generally rather high, 1.033-1.036 with 90-100 p. m. solids. The principal constituents are the albuminous bodies of blood-serum. The rare tubo-ovarial cysts contain as a rule a. watery, serous fluid containing no pseudomucin. The parovarial cysts or the CYSTS of the LIGAMENTA LATA may attain a considerable size. In general the contents are watery, mostly very pale yellow-colered, water-clear or only slightly opales- cent liquids. The specific gravity is low, 1.002-1.009 ; and the solids only amount to 10-20 p. m. Pseudomucin does not occur as a typical constituent ; albumin is sometimes absent, and when it does occur the quantity is very small. The principal part of the solids consist of salts and extractive bodies. In exceptional cases the fluid may be rich in albumin, and may show a higher specific gravity. * The Egg. The small ova of man and mammalia cannot, for evident rea- sons, be the subject of a searching chemical investigation. Up to the present time the eggs of birds, amphibians, and fishes have been investigated, but above all the hen's egg. We will here occupy ourselves with the constituents of this last. The yolk of the hen's egg. In the so-called white yolk, which forms the germ with a continuation reaching to the centre of the yolk (latebra) and also a layer found between the yolk and yolk- membrane, we find albumin, nuclein, lecithin, and potassium (LIEBERMANN). The occurrence of glycogen is doubtful. The yolk-membrane consists of an albumoid similar in certain respects to keratin (LiEBERMAtftf). % , The principal part of the yolk — the nutritive yolk or yellow — is a viscous, non-transparent, pale- or orange-yellow alkaline emulsion of a mild taste. The yolk contains vitellin, lecithin, cholesterin, fat, coloring matters, traces of neuridin (BRIEGER), glucose in very small quantities, and mineral bodies. The occurrence of cerebrin 292 PHYSIOLOGICAL CHEM1STR7. and of granules similar to starch (DABESTE) has not been positively proved. Ovovitellin. This body is generally considered as a globulin, but it resembles a nucleoalburnin more. The question as to what relationship other proteid substances, such as the aleurone crystals of certain semen, and the so-called " dotterpldttchen " in the eggs of certain fishes and amphibians which are related to ovovitellm, bear to this substance, is a question which requires further investi- gation. The ovovitellin which has been prepared from the yolk of eggs is not a pure albuminous body, but always contains lecithin. HOPPE-SEYLEB found 25$ lecithin in vitellin and also some nuclein. The lecithin may be removed by boiling alcohol, but the vitellin is changed thereby and it is therefore probable that the lecithin is chemically united with the vitellin (HOPPE-SEYLEB). BUNGE prepared a nuclein by digesting the yolk with gastric juice, and this nuclein, according to him, is of great importance in the formation of the blood, and on these grounds he called it hcematogen. This haematogen — whose composition is as follows: 0 42.11, H 6.08, N 14.73, S 0.55, P 5.19, Fe 0.29 and 0 31.05$ seems to be a product of the decomposition of vitellin. Vitellin is similar to the globulins in that it is insoluble in water ; but on the contrary soluble in dilute neutral-salt solutions (although the solution is not quite transparent). It is also soluble in hydrochloric acid of 1 p. m. and in very dilute solutions of alka- lies or alkali carbonates. It is precipitated from its salt solution by diluting with water, and when allowed to stand some time iu contact with water the vitellin is gradually changed, forming a sub- stance more like the albuminates. The coagulation temperature for the solution containing salt (NaCl) lies between -|- 70° and 75° C. or, when heated very rapidly, at about + 80° C. Vitellin differs from the globulins in yielding nuclein by pepsin digestion. On this account vitellin, as previously stated (page 14), is considered as a nucleoalbumm even though it differs from them in general by the coagulation of its neutral solutions containing salt, at a tem- perature below 100° C. ; also by certain solubilities and precipita- tions. Because of the difficulty of removing lecithin without changing the properties of vitellin it is necessary, since lecithin may ORGANS OF GENERATION. 293 essentially change the solubility and precipitating capacity of the albuminous body, to wait for further investigations before we place vitellin definitely in the globulin or nucleoalbumin group. The chief points in the preparation of ovo vitellin are as follows: The yolk is thoroughly agitated with ether; the residue is dissolved in a 10$ common-salt solution, filtered, and the vitellin precipitated by adding an abundance of water. The vitellin is now purified by repeatedly redissolving in dilute common-salt solutions and precipi- tating by water. The yolk also contains, besides vitellin, alkali-albuminate and albumin. The fat of the yolk of the egg is, according to LIEBERMANN, a mixture of a solid and a liquid fat. The solid fat consists chiefly of tripalmitin with some stearin. On the saponification of the egg-oil LIEBERMANN obtained 40$ oleic acid, 38.04$ palmitic acid and 15.21$ stearic acid. The fat of the yolk of the egg contains less carbon than other fats, which may depend on the presence of mono and diglycerides or on a quantity of fatty acid deficient in carbon (LIEBERMANN). Lutein. Yellow or orange-red amorphous coloring matters occur in the yellow of the egg and in several other places in the animal organism, for instance in the blood-serum and serous fluids, fatty tissues, milk-fat, corpora lutea, and in the fat-globules of the retina. These coloring matters, which also occur in the vegetable kingdom (THUDICHUM), have been called luteines or lipocfiromes. The luteines, which among themselves show somewhat different properties, are all soluble in alcohol, ether, and chloroform. They differ from the bile-coloring matter, bilirubin, in that they are not separated from their solution in chloroform by water containing alkali, and also in that they do not give the characteristic play of colors with nitric acid containing a little nitrous acid, but give a transient blue color, and lastly they give an absorption-spectrum of ordinarily two bands, of which one covers the line F and the other lies between the lines F and G. The luteines withstand the action of alkalies so that they are not changed when we remove the fats present by means of saponification. The lutein from the yolk of the egg has not been prepared in a pure state. According to CHEVREUL and GOBLET, the yolk contains a partly red and part- ly yellow pigment. MALY has found two pigments free from iron in the eggs 294 PHYSIOLOGICAL CHEMISTRY. of the water-spider (maja squinado), one a red, mtellorubin, and the other a yel- low pigment, mtettolutein. Both of these pigments are colored blue by nitric acid containing nitrous acid, and beautifully green by concentrated sulphuric acid. The absorption-bands, especially of the vitellolutein, correspond very nearly with those of ovolutein. The mineral bodies of the yolk of the egg consist, according to POLECK, of 51.2-65.7 parts soda, 89.3-80.5 potash, 122.1-132.8 lime, 20.7-21.1 magnesia, 14.5-11.90 iron oxide, 638.1-667.0 phos- phoric acid, and 5.5-14.0 parts silicic acid in 1000 parts of the ash. We find phosphoric acid and lime the most abundant, and then potash, which is somewhat greater in quantity than the soda. These results are not, however, quite correct, first, because no dissolved phosphate occurs in the yolk (LIEBERMANN), and secondly, in burning, phosphoric and sulphuric acids are produced and these drive away the chlorine, which is not accounted for in the pre- ceding analyses. The yolk of the hen's egg weighs about 12-18 grms. The quantity of water and solids amounts, according to PARKES, to 471.9 p. m. and 528.1 p. m. respectively. Among the solids he found 156.3 p. m. albumin, 3.53 p. m. soluble and 6.12 p. m. in- soluble salts. The quantity of fat, according to PARKES, is 228.4 p. m,, the lecithin, calculated from the amount of phosphorus in the organic substance in the alcohol-ether extract, was 107.2 p. m., and the cholesterin 17.5 p. m. The white of the egg is a faint-yellowish albuminous fluid en- closed in a framework of thin membranes ; and this fluid is in it- self very liquid, but seems viscous because of the presence of these fine membranes. That substance which forms the membranes, and of which the chalaza consists, seems to be a body nearly related to horn substances (LIEBERMANN"). The white of the egg has a specific gravity of 1.045 and always nas an alkaline reaction. It contains 850-880 p. m. water, 100-130 p. m. albuminous bodies, and 7 p. m. salts. Among the extractive bodies LEHMAN^ found a fermentable kind of sugar which amounted to 5 p. m., or according to MEISSNER, 80 p. m. Besides these, we find in the white of the egg traces of fats, soaps, lecithin, and cholesterin. The white of the egg during incubation becomes transparent on boiling and acts in many respects like alkali-albuminate. This albumin TARCHANOFF called "tatalbumin." ORGANS OF GENERATION. 295 The albuminous bodies of the white of the egg belong partly to the globulin and partly to the albumin group. The egg-globulin is, according to DILLNER, closely related to serum-globulin. On diluting the white of the egg with water it partly separates. It is also precipitated by magnesium sulphate. The quantity of globulins in the white of the egg is on an average 6.67 p. m., or about 67 p. m. of the total proteids. According to CORIK and BERAKD, we have two globulins in the white of the egg, one coagulating at -f 57.5° C., and the other at -J- 67° C. Ovalbumin, or the albumin of the white of the egg. The com- position of this albumin is C 52.25, H 6.9, N 15.25, and S 1.93#. The ovalbumin has the properties of the albumins in general, but differs from serum-albumin in the following : The specific rotary power is lower a(D) = — 38°. Solutions containing medium amounts of the albumin coagulate at -\- 56° C., irrespective whether the amount of salt present is large or small. On the contrary, the temperature of coagulation changes with a constant amount of salt, but with variable amounts of albumin in the solution. Ovalbumin is quickly rendered insoluble by alcohol. It is precipitated by a sufficient quantity of nitric or hydrochloric acid, but it dissolves with more difficulty in an excess of these acids than the serum -al- bumin. Ovalbumin in solution, when introduced into the blood- circulation, passes into the urine, which is not the case with serum- albumin. According to GAUTIER and BECHAMP, ovalbumin is a mixture of two albumins with a coagulation temperature 60°-63° and 71°-74° C. respectively. According to CORIN and BERARD, it is a mixture of three albumins with a coagulation-temperature respectively 67°, 72°, and 82° C. Perhaps the fact has been overlooked that the coagulation -temperature of ovalbumiu is changed by variable amounts of albumin in solution. Ovalbumin is obtained by precipitating the globulins with MgS04 at + 20° C. and saturating the filtrate with Na2S04 at the same temperature. The ovalbumin which separates is filtered, pressed, dissolved in water, and freed from salts by dialysis. The dialyzed solution is then evaporated in a vacuum or at 40°-50° C. If precipitated with alcohol, albumin becomes quickly insoluble. The mineral bodies of the white of the egg have been analyzed by POLECK and WEBER. They found in 1000 parts of the ash : 276.6-284.5 grms. potash, 235.6-329.3 soda, 17.4-29 lime, 16-31.7 296 , PHYSIOLOGICAL CHEMISTRY. magnesia, 4.4-5.5 iron oxide, 238.4-285.6 chlorine, 31.6-48.3 phos- phoric acid (P205), 13.2-26.3 sulphuric acid, 2.8-20.4 silicic acid and 96.7-116 grms. carbon dioxide. Traces of fluorine have also been found (NICKLES). The ash of the white of the egg contains, as compared with the yolk, a greater amount of chlorine and alka- lies, and a smaller amount of lime, phosphoric acid, and iron. The Shell-membrane and the Egg-shell. The shell-membrane consists, as above stated (page 35), of a keratin substance. The shell contains very little organic substance, 36-65 p. m. The chief mass, more than 900 p. m., consists of calcium carbonate; besides this there are very small amounts of magnesium carbon- ate and earthy phosphates. The different coloring of birds' eggs depends, as WICKE, SORBY, LIEBER- MANN and KRUKENBERG have shown, upon several coloring matters. Among these we find a red or reddish-brown pigment called "oorodein" by SORBY, which is perhaps identical with haematoporphyrin (SORBY, KRUKENBERG). The green or blue coloring matter, SORBY'S oocyan, seems, according to LIEBERMANN, and KRUKENBERG, to be partly biliverdin and partly a blue derivative of the bile-pigments. The eggs of birds have a space at their blunt end filled with gas ; this gas contains on an average 23.3 vols. per cent oxygen (BISCHOFF). The weight of a hen's egg varies between 40-60 grammes and may weigh sometimes 70 grms. The shell and shell-membrane to- gether when carefully cleaned, but still in the moist state, weigh 5-8 grms. The yolk weighs 12-18 and the white 23-34, or about double. The white of the egg of cartilaginous and bone fishes contains only traces of true albumin, and the cover of the frog's egg consists, according to GTACOSA, of mucin. The crystalline formations (yolk-spherules or dotterpldttchen) which have been observed in the egg of the tortoise, frog, ray, shark, and other fishes, and which are described by VALENCIENNES and FREMY under the names, emydin, ichthin, icfithidin, and ichthulin, contain lecithin, nuclein, and albumin. They probably correspond to the yellow yolk-globule in the nutri- tive yolk of the hen's egg. The egg of the river crab and the lobster contain the same pigment as the shell of the animal. This pigment, called cyano- erystallin, becomes red on boiling in water. In fossil eggs (of APTENODYTES, PELECANUS, and HALI^EUS) in old guano deposits a yellowish-white, silky, lamiated combination, has been found •which is called guanoyulit, (NH4)2SO4 -f 2K2SO4 + 3KHSO4 + 4HaO, and which is easily soluble in water but is insoluble in alcohol and ether. Those eggs which develop outside of the mother-organism must contain all the elements necessary for the young animals. One OKGANS OF GENERATION. 297 finds, therefore, in the yolk and white of the egg an abundant quan- tity of albuminous bodies of different kinds, and especially a phos- phorized albumin in the yolk. Further, we also find lecithin in the yolk, which seems to habitually occur in the developing cell. The occurrence of glycogen is doubtful, and the carbohydrates, which have no direct value as tissue-builders, are perhaps represented by a very small amount of glucose. On the contrary, the egg contains a large proportion of fat, which doubtless is an important source of nutrition and respiration for the embryo. The cholesterin and the lutein can hardly have a direct influence on the development of the embryo. The egg also seems to contain the mineral bodies neces- sary for the development of the young animal. The lack of phos- phoric acid is compensated by an abundant amount of phosphorized organic substance, and the nucleoalbumin containing iron, from which the haematogen (see page 292) is formed, is doubtless, as BUNGE claims, of great importance in the formation of the haemo- globin containing iron. The silicic acid necessary for the develop- ment of the feathers is also found in the egg. During the period of incubation the egg loses weight, chiefly due to loss of water. The quantity of solids, especially the fat and the albumins, diminishes and the egg gives off not only carbon dioxide, but also, as LIEBERMANN has shown, nitrogen or a nitrogenized substance. This loss is compensated by the absorption of oxygen, and it is found that during incubation a respiratory exchange of gas takes place. While the quantity of dry substance in the egg during this period always decreases, the quantity of mineral bodies, ulbumin, and fat always increases in the embryo. The increase in the amount of fat in the embryo depends, according to LIEBER- MANN, in great part upon a taking up of the nutritive yolk in the abdominal cavity. The weight of the shell and the quantity of lime salts contained therein remains unchanged during incubation. The yolk and white together contain the necessary quantity of lime for development. The most complete and careful chemical investigation on the development of the embryo of the hen has been made by LIEBER- MANN. From his researches we may quote the following: In the earlier stages of the development, tissues very rich in water are formed, but on the continuation of the development the quantity of 298 PHYSIOLOGICAL CHEMISTRY. water decreases. The absolute quantity of bodies soluble in water increases with the development, while their relative quantities, as compared to the other solids, continually decreases. The quantity of bodies soluble in alcohol quickly increases. A specially impor- tant increase is noticed in the fat, whose quantity is not very great even on the fourteenth day, but after that it becomes considerable. The quantity of albuminous bodies and albuminoid insoluble in water grows continually and regularly in such a way that their absolute quantity increases while their relative quantity remains nearly un- changed. LIEBEBMANN found no glutin in the embryo of the hen. The embryo does not contain any gelatin-forming substance until the tenth day, and from the fourteenth day on it contains a body which when boiled with water gives a substance similar to chondrin. A body similar to mucin occurs in the embryo when about six days old, but then disappears. The quantity of haemoglobin shows a continual increase compared to the weight of the body. LIEBER- MANN found that the relationship of the haemoglobin to the bodily weight was 1: 728 on the eleventh day and 1: 421 on the twenty- first day. The tissue of the placenta has not thus far been the subject of detailed chemical investigations. In the edges of the placenta of bitches and of cats a crystallizable orange-colored pigment (bilirubin ?) has been found, and also a green amorphous pigment, MECKEL'S Tuxmatochlorin, which is considered as biliverdin by ETTI. PREYER questions the identity of these pigments with biliverdin. From the cotyledons of the placenta in ruminants a white or faint rose- colored creamy fluid, the uterine milk, can be obtained by pressure. It is al kaline in reaction, but becomes acid quickly. Its specific gravity is 1.033- 1.040. It contains as form-elements fat-globules, small granules, and epithe- lium-cells. We have found 81.2-120.9 p. m. solids, 61.2-105.6 p. m. albumin, about 10 p. m. fat, and 3.7-8.2 p. m. ash in the uterine milk. The fluid occurring in the so-called GRAPE-MOLE (mola racemosa) has a low specific gravity, 1.009-1.012, and 19.4-26.3 p. m. solids with 9-10 p. m. protein bodies and 6-7 p. m. ash. The amniotic fluid is in women thin, whitish, or pale yellow ; sometimes it is somewhat yellowish brown and cloudy. White flakes separate. The form-elements are mucus-corpuscles, epithe- lium-cells, fat-drops, and lanugo hair. The odor is stale, the reaction neutral or faintly alkaline. The specific gravity is 1.002-1.028. The amniotic fluid contains the constituents of ordinary transu- dations. The amount of solids at birth is hardly 20 p. m. In the earlier stages of pregnancy the fluid contains more solids, especially ORGANS OF GENERATION, f^ 299 proteids. Among the albuminous bodies WEYL found one sub- stance similar to vitellin and with great probability also serum albumin, besides small quantities of mucin. Glucose is regularly found in the amniotic fluid of cows, but not in human beings. On the contrary, the human amniotic fluid contains some urea and allantoin. The quantity of these may be increased in hydramnion (PROCHOWNICK, HARNACK), which depends on an increased secre- tion by the kidneys and skin of the foetus. Creatin and lactates are questionable constituents of the amniotic fluid. The quantity of urea in the amniotic fluid is, according to PROCHOWNICK, 0.16 p. m. In the fluid in hydramnion PROCHOWNICK and HARNACK found respectively 0.34 and 0.48 p. m. urea. The chief mass of the solids consists of salts. The quantity of chlorides (NaCl) is 5.7-6.6 p. m. « 300 PHYSIOLOGICAL CHEMISTRY. CHAPTER XII. MILK. THE chemical constituents of the mammary glands have beei* little studied. The protoplasm of the cells is rich in proteids and, as is ^generally admitted, consists in great part of casein or a sub- stance nearly related, l^all the milk is removed from the mam- mary gland by thorough washing, the cells still contain a large quantity of proteids which swell up to a slimy, ropy, or fibrous mass when very dilute alkali (1-2 p. m. KOH) is added. These proteids consists mainly of nucleoalbumin, which is gradually changed by the action of the alkali. If the mammary gland is boiled with water, the protoplasm of the cell is decomposed and a nucleoalbumin passes into solution, which may be precipitated by the addition of acetic acid, and which is characterized by its greater insolubility in acetic acid, compared with casein. This nucleo- albumiu, which may well be considered as a protoplasm-nucleo- albumin changed by heat, also gives on boiling with dilute mineral acids a reducible substance whose nature is not known. The relation the above-mentioned nucleoalbumin, which is more cor- rectly designated as a proteid when the mother-substance of the reducible body does not occur as an impurity, bears to the sugar of milk or the mother-substance of the same, has not been determined. According to BERT, the secreting glands contain a body which on boiling with dilute mineral acids yields a reducible substance. Such a substance, which acts as a step towards the formation of milk-sugar, has also been observed by THIERFELDER. Fat seems to be a never-failing constituent of the cell, at least in the secreting gland, and this fat may be observed in the protoplasm as large or small globules similar to milk-globules. The extractive bodies of MILK. 301 the mammary glands have been little investigated, but among them we find considerable amounts of xanthin bodies. As human milk and milk of animals are essentially of the same constitution, it seems best to speak first of the one most thoroughly investigated, namely, cow's milk, and then of the essential proper- ties of the remaining important varieties of milk. Cow's Milk. Cow's milk forms, as all milks do, an emulsion which consists of very finely-divided fat suspended in a solution consisting chiefly of albuminous bodies, milk-sugar, and salts. Milk is non-trans- parent, white, whitish yellow, or in thin layers somewhat bluish white, of a faint, insipid odor and mild, faintly-sweetish taste. The reaction is regularly amphoteric, sometimes with a stronger action on the red and sometimes on the blue litmus-paper. The specific gravity is 1.028 to 1.0345 at + 15° C. Milk gradually changes when exposed to the air, and its reaction becomes more acid. This depends on a transformation of the milk-sugar into lactic acid, which is produced partly by the pres- ence of a special enzyme originating in the glands but not yet positively detected, but which is chiefly produced by micro- organisms. Entirely fresh, amphoteric milk does not coagulate on boiling, but forms a skin consisting of coagulated casein and lime-salts, which rapidly re-forms after being removed. Even after passing a current of carbon dioxide through the fresh milk it does not coagu- late on boiling. In proportion as the formation of lactic acid ad- vances this behavior changes, and soon a stage is reached when the milk, which has previously had carbon dioxide passed through it, coagulates on boiling. At a second stage it coagulates alone on heating ; then it coagulates by passing carbon dioxide alone with- out boiling; and lastly, when the formation of lactic acid is suffi- cient, it coagulates spontaneously at the ordinary temperature, forming a solid mass. It may also happen, especially in the warmth, that the casein-clot contracts and a yellowish or yellowish-green acid liquid (acid whey) is separated. If the milk is sterilized by heating and contact with micro- 302 PHYSIOLOGICAL CHEMISTRY. organisms prevented, the formation of lactic acid may be entirely stopped. The formation of acid may also be prevented, at least for some time, by many antiseptics, such as salicylic acid (1:5000), thymol, boracic acid, and other oodles. If milk is allowed to stand for a long time at a temperature of 0° C. it re- mains fluid for several weeks, but coagulates at last. In this case the coagula- tion is not caused by the formation of lactic acid, but is more likely due to the formation of fatty acids caused by an oxidation (HOPPB-SEYLER). If freshly-milked amphoteric milk is treated with rennet, it coagulates quickly, especially at the temperature of the body, to a solid mass (cheese), from which a yellowish fluid (sweet whey) is gradually pressed out. This coagulation of milk occurs without any change in its reaction ; it may also take place with the very faintest alkaline reaction; therefore it is distinct from the acid coagulation. Milk sometimes undergoes a peculiar kind of coagulation, being converted into a thick, ropy, slimy mass (thick milk). This conversion depends, accord- ing to SCHMIDT-MULHEIM, upon a peculiar change of the milk-sugar in which this last is converted into a slimy product. This conversion is caused by a special organized ferment. In cow's milk we find as form-elements a few colostrum cor- puscles (see Colostrum) and a few pale nucleated cells. The number of these form-elements is very small compared with the immense amount of the most essential form-constituents, the milk- globules. The Milk-globules. These consist of extremely small drops of fat whose number is, according to BOHR, 2.6-11.4 or an average of 5.6 million in 1 c. mm. and whose diameter is 0.00014-0.0063 mm. (BOHR). It is unquestionable that milk-globules contain fat, and we consider it as positive that all the milk-fat is found in them. Another and disputed question is whether milk-globules consist entirely of fat or whether they also contain albumin. According to an observation of ASCHERSON, the drops of fat are covered with a fine albuminous coat, a so-called hapto gen-membrane, when placed in an alkaline albumin solution. As milk on shaking with ether does not give up its fat, or only very slowly by a great excess of ether, and as this takes place very easily after the addition of acids or alkalies which dissolve albumin, it was formerly thought that the fat-globules of the milk were enveloped in an albuminous MILK. coat. A true membrane has not been detected ; and since, when no means of dissolving the albumin is resorted to — for example, when the milk is precipitated by carbon dioxide after the addition of very little acetic acid (SOXHLET), or when it is coagulated by rennet — the fat can be very easily extracted by ether, the theory of a special albuminous membrane for the fat-globules has been gener- ally abandoned. The observations of QUINCKES on the behavior of the fat-globules in an emulsion prepared with gum have led, at the present time, to the conclusion that each fat-globule in the milk is surrounded by a stratum of casein solution by means of molecular attraction, and this prevents the globules from uniting with each other. Everything that changes the physical property of the casein in the milk or precipitates it must necessarily help the solution of the fat in ether, and it is in this way that the alka- lies, acids, and rennet work. If we accept this view, which requires further proof, we must not over- look the fact that the fat-globules remain unchanged when the milk under agitation is coagulated with rennet. In this case we find an immense amount of unchanged milk-globules in the whey, and if we wish to admit of a stratum of proteids around the fat-globules proceeding from the molecular attraction, we must not consider that it is entirely due to casein, but also to albumin. If the fat-globules are filtered off and washed on a filter, we always obtain (RADENHAUSEN and DANILEWSKY) after their treatment with ether a residue consisting of albumin. From this behavior the deduction has been made that the fat-globules, even though they have no real membrane, consist, neverthe- less, of fat and albumin. The extreme difficulty of completely removing the albuminous bodies of the milk by washing the fat on the filter renders it necessary to exercise great caution in drawing a conclusion. The question as to the composition of the milk-globules, and especially as to the possible amount of albumin, cannot be decided at present. The milk-fat has a rather variable specific gravity, which accord- ing to BOHR, is 0.949-0. 996 at — 15° C. The milk-fat, which is ob- tained under the name of butter, consists in great part of the neutral fats palmitin, olein, and stearin. Besides these it contains, as tri-glycerides, small quantities of butyric acid and caproic acid, traces of caprylic and capric acids (lauric acid probably also occurs), myristic and arachidic acids. Butter which has been exposed to the action of sunlight contains also formic acid (DucLAUX). Milk-fat also contains a small quantity of lecithin and cholesterin, also a yellow coloring matter. The quantity of volatile fa£ty acids in butter is, according to DUCLAUX, on an average about 70 p. m., of which 37-51 p. in. is butyric acid and 20-33 p. m. is caproic 304 PHYSIOLOGICAL CHEMISTRY. acid. The non-volatile fat consists of -f^ to -fy olein, and the re- mainder of a mixture of palmitin and stearin. The quantity of volatile fatty acids in butter-fat is of great practical im- portance in the methods for detecting the presence of foreign fats in butter. This detection is performed generally according to REICHEKT'S process based on HEHNER and ANGELL'S method. The fat is saponified with alcoholic potash and the alcohol evaporated. The soaps are dissolved in water, and then distilled with an excess of phosphoric acid. The quantity of volatile fatty acids in the distillate is determined by titration with deci-normal alkali. With butter of proper composition 2.5 grms. should yield a distillate requiring 14-13 cc. for neutralization, and at least not less than 12 cc. of the deci-normal alkali. In proportion as the butter contains a greater quantity of foreign fats the quantity of alkali required becomes smaller. The milk-plasma, or that fluid in which the fat-globules are sus- pended, contains at least three different albuminous bodies, casein, lactoglobulin, and lactalbumin, and two carbohydrates, of which only one, the milk-sugar, is of great importance. The milk-plasma also contains extractive bodies, traces of urea, creatin, creatinin, liypo- xantliin (?), lecithin, cholesterin, about 1 p. m. citric acid (SOXHLET and HENKEL) ; a still greater amount according to SOLDNER, and lastly also mineral bodies and gases. Casein. This protein substance, which thus far has been de- tected positively in milk, belongs to the nucleoalbumins, and differs from the albuminates by its containing phosphorus and by its be- havior with the rennet enzyme. Casein from cow's milk has the following composition: C 53.0, H 7.0, N 15.7, S 0.8, P 0.85, and 0 22.65 per cent. Its specific rotation is, according to HOPPE- SEYLER somewhat variable ; in neutral solution it is a(D) = — 80°. The question whether the casein from different varieties of milk is identical or if there are several different caseins has not been posi- tively determined. Casein, when dry, appears like a fine white powder which, after heating to 100° C. or somewhat above, shows the properties and solubilities of freshly-precipitated, still-moist casein. Casein is only slightly soluble in water or in neutral-salt solutions. It acts like a rather strong acid, dissolves readily in water on the addition of very little alkali, forming a neutral or acid liquid, and lastly it dissolves in water in the presence of calcium carbonate, from which it expels the carbon dioxide. If casein is dissolved in lime-water and this solution treated with very dilute phosphoric acid until it is neutral MILK. 305 in reaction, the casein appears to remain in solution, but- is prob- ably only swollen as in milk, and the liquid contains at the same time a large quantity of calcium phosphate without any precipitate or any visible suspended particles. The casein solutions containing lime are opalescent and have on warming the appearance of milk deficient in fat. Therefore it is not impossible that the white color of the milk is due partly to the casein and calcium phosphate. Casein solutions do not coagulate on boiling, but are covered, as milk, with a skin. It is precipitated by very little acid, but the presence of neutral salts retards the precipitation. A casein solu- tion containing salt, or ordinary milk, requires, therefore, more acid for precipitation than a salt-free solution of casein of the same con- centration. The precipitated casein dissolves very easily again in a small excess of the acid, but less easily in an excess of acetic acid. The acid solutions are precipitated by mineral acids in excess. Casein is precipitated from neutral solutions or from milk by common salt or magnesium sulphate in substance without changing its properties. Metallic salts, such as copper sulphate, completely precipitate the casein from neutral solutions. The property which is the most characteristic of casein is that it coagulates with rennet in the presence of a sufficiently great amount of lime-salts. In solutions free from lime-salts the casein does not coagulate. According to SOXHLET and SOLDNER, the soluble lime- salts are only of essential importance in coagulation, while the cal- cium phosphate is without importance. The chemical processes which take place in the rennet coagulation have not been thorough- ly investigated ; still several observations seem to show that casein splits partly into a difficultly soluble body paracasein or cheese, whose composition closely resembles that of casein and which forms the chief product, and partly into an easily-soluble substance, similar to albumose, whey-albumin, which is deficient in carbon and nitrogen (50. 3# C and 13.2$ N, KOSTNEB) and which is produced in very small quantities. Paracasein is not further changed by the rennet enzyme, and it has not the same property of holding calcium phosphate in solution as casein has. If rennet be added to a casein solution free from lime, the solution does not coagulate, but the casein is changed so that the solution, after the enzyme has been destroyed by rapid heating, acts like a paracasein solution, on the 306 PHYSIOLOGICAL CHEMISTRY. addition of lime-salts after cooling. The action of the rennet enzyme on casein takes place also in the absence of lime-salts, and these last are only necessary for the coagulation or the precipitation of the paracasein. Casein may be prepared in the following way : The milk is di- luted with 4 vols. water and the mixture treated with acetic acid to 0.75 to 1 p. m. Casein thus obtained is purified by repeated solu- tions in water with the aid of the smallest quantity of alkali pos- sible, by filtrating and reprecipitating with acetic acid, and thoroughly washing with water. Most of the milk-fat is retained by the filter on the first filtration, and the casein contaminated with traces of fat is purified by treating with alcohol and ether. Lactoglobulin was obtained by SEBELIEX from cow's milk by saturating it with NaCl in substance (which precipitated the casein), and saturating the filtrate with magnesium sulphate. As far as it has been investigated it had the properties of serum-glob- ulin, with which it is perhaps identical. Lactalbumin was first prepared in a pure state from milk by SEBELIEN. Its composition is, according to SEBELIEN, C 52.19, H 7.18, N 15.77, S 1.73, 0 23.13 per cent. Lactalbumin has the properties of the albumins. It coagulates, according to the con- centration and the amount of salt in solution, at -f 72° to 84° C. It is similar to serum albumin, but differs from it in having a con- siderably lower specific rotary power; a (D) = — 37°. The principle of the preparation of lactalbumin is the same as for the preparation of serum-albumin from serum. The casein and the globulin are removed by MgS04 in substance and the filtrate treated as previously stated (page 61). The occurrence of other albuminous bodies, such as albumoses and peptones, in milk has not been positively proved. These bodies are easily produced as laboration products from the other albuminous bodies of the milk. Such a laboration product is MILLON'S and COMAILLE'S lactoprotein, which is a mix- ture of a little casein with changed albumin, and which is formed by the chemical operations. Milk-sugar, LACTOSE, CttHyf>u + H20. This sugar with the absorption of water can be split into two glucoses, dextrose and galactose. It yields by the action of dilute nitric acid, besides carbon dioxide, oxalic acid, tartaric acid, saccharic acid, and racemic acid, a crystallizable mucic acid, which is nearly insoluble in cold water and in alcohol, and which may also be obtained from dulcite, MILK. 307 gum, and vegetable mucus. By the action of sodium amalgam on milk-sugar we obtain dulcite, mannite, lactic acid, and other pro- ducts. By the action of alkalies we obtain lactic acid among other products. Milk-sugar occurs in all milks. It has also been found in the urine of pregnant women. According to BOUCHARDAT, it also occurs in the ripe fruit of the achras sapota. Milk-sugar occurs ordinarily as colorless rhombic crystals with 1 mol. of water of crystallization, which is driven off by slowly heat- ing to 100° C., but more easily at 130°-140° C. At 170° to 180° C. it is converted into a brown amorphous mass, lactocaramel, C6H1005. Milk-sugar dissolves in 6 parts cold and in 2.5 parts boiling water; it has a faint sweetish taste. It does not dissolve in ether or abso- lute alcohol. Its solutions are dextro-gyrate. The rotary power, which, on heating the solution to 100° C. becomes constant, is a (D) = -\- 52.5°. Milk-sugar combines with bases ; the alkali combina- tions are insoluble in alcohol. Milk-sugar is not fermentable with pure yeast. It undergoes, on the contrary, alcoholic fermentation by the action of certain schizomycetes, and lactic acid is then produced thereby. The prep- aration of milk-wine, " kumyss," from mare's milk and "kephir" from cow's milk is based upon this fact. Micro-organisms pro- duce a lactic fermentation in lactose, and this explains the ordinary souring of milk. Lactose responds to the reactions of grape-sugar, such as MOORE'S or TROMMER'S and the bismuth test, which will all be described in Chapter XIV on the urine. It also reduces mercuric oxide in alkaline solutions. After warming with phenylhydrazin acetate it gives on cooling a yellow crystalline precipitate of phenyl- lactosazon, C^H^N^Og (see Chap. XIV on sugar in the urine). It differs from cane-sugar by giving positive reactions with MOORE'S and the bismuth test, and also that it does not darken when heated with anhydrous oxalic acid to 100° C. It differs from grape-sugar and maltose by its solubility and crystalline form ; but especially by its not fermenting with yeast and by yielding mucic acid with nitric acid. For the preparation of milk-sugar we make use of the by-product in the preparation of cheese, the sweet whey. The albumin is re- 308 PHYSIOLOGICAL CHEMISTRY. moved by coagulation with heat and the nitrate evaporated to a syrup. The crystals which separate after a certain time are recrystal- lized from water after decolorizing with animal charcoal. A pure preparation may be obtained from the commercial milk-sugar by repeated recrystallization. The quantitative estimation of milk- sugar may in part be performed by the polaristrobometer and partly by means of titration with FEHLIN G'S solution. 10 cc. of FEELING'S solution corresponds to 0.067 grm. milk-sugar (in regard to FEH- solution and the titration of sugar, see Chapter XIV). RITTHATJSEN has found another carbohydrate in milk which is soluble in water, non-crystallizable, which has a faint reducing action, and which yields on boiling with an acid a body having a greater reducing power. LAND- WEHR considers this as an animal gum. The mineral bodies of milk will be treated in connection with its quantitative composition. The methods for the quantitative analysis of milk are numerous, and as they all cannot be treated of here, we will give the chief points of a few of the most trustworthy and most frequently- employed methods. In determining the solids a carefully- weighed quantity of milk is mixed with an equal weight of heated sand, fine glass powder, or asbestos. The evaporation is first done on the water-bath and finished in a current of carbon dioxide or hydrogen not above 100° C. The mineral bodies are determined by ashing the milk, using the precautions suggested in the text-books. The results obtained for the phosphoric acid are incorrect on account of the burning of phosphorized bodies, such as casein and lecithin. We must there- fore, according to SOLDNER, subtract 25$ from the total phosphoric acid found in the milk. The quantity of sulphate in the ash also depends on the burning of the albumins. In the determination of the total amount of albuminous bodies we make use of RITTHAUSEN'S method, namely, precipitate the milk with copper sulphate. This method gives incorrect results because the copper hydroxide does not give up all its water of hydration on drying the precipitate, but only after ashing the same. The results for the proteids are therefore somewhat too high. The method of PULS and STENBERG consists in first diluting the neutralized milk with some water and then treating with alco- hol until the mixture contains 70-85 vols. per cent alcohol. The precipitate is collected on a filter, washed with warm 70$ alcohol, extracted with ether, dried, weighed, burnt, and the residue re- weighed. The traces of albumin which remain in the filtrate and MILK. 309 wash-liquor are precipitated by tannic acid (see page 21). 63# of the tannic acid precipitate is considered as albumin, and this must be added to the albumin found directly. This method gives exact and good results. According to the method of SEBELIEN, 3-5 grms. of milk are diluted with an equal volume of water, a little common-salt solution added, and precipitated with an excess of tannic acid. The precipi- tate is washed with cold water, and then the quantity of nitrogen determined by KJELDAHI/S method. The total nitrogen found when multiplied by 6.37 (casein and lactalbumin contain both 15.7$ nitrogen) gives the total quantity of albuminous bodies. This easily -executed method gives very good results, but until the quan- tity of nitrogen in the albuminous bodies of other varieties of milk has been exactly determined this method cannot be used except for the analysis of cow's milk. To determine the casein and albumins separately we may make use of the method first suggested by HOPPE-SEYLER and TOLMAT- SCHEFF, in which the casein is precipitated by magnesium sulphate. According to SEBELIEN, the milk is diluted with its own volume of a saturated magnesium-sulphate solution, then saturated with the salt in substance, the precipitate filtered and washed with a saturated magnesium-sulphate solution. The nitrogen is deter- mined in the precipitate by KJELDAHI/S method, and the quantity of casein determined by multiplying the result by 6.37. The quan- tity of lactalbumin may be calculated as the difference between the casein and the total albumin found. The lactalbumin may also be precipitated by tannic acid from the filtrate containing MgSO* from the casein precipitate, diluted with water, and the nitrogen deter- mined by KJELDAHI/S method and the result multiplied by 6.37. SEBELIEN'S method is only suited for cow's milk. The quantity of globulins in milk cannot be exactly determined. A minimum result can be obtained by first precipitating the casein completely by NaCl in substance, and then precipitating the glob- ulins in the filtrate by magnesium sulphate (SEBELIEN). The casein may also be precipitated from the diluted milk, and the globulin precipitated after neutralization by means of MgS04. In these cases we obtain somewhat high results, because of the pres- ence of traces of casein which remain behind. The fat is determined directly by thoroughly extracting the dried milk with- ether, evaporating the ether from the extract, and weighing the residue. The fat may be determined by aerometric means by adding alkali to the milk, shaking with ether, and deter- mining the specific gravity of the fat solution by means of SOXH- LET/S apparatus. In determining the amount of fat in a larsre number of samples the lactokrit of DE LAVAL may be used with success. The milk is first mixed with an equal volume of a mix- 310 PHYSIOLOGICAL CHEMISTRY. ture of acetic acid and concentrated sulphuric acid, warmed 7-8 minutes on the water-bath, the mixture placed in graduated tubes, and these in the centrifugal machine at -j- 50° C. The height of the layer of fat gives its quantity. In determining the milk-sugar first the albumin is removed. For this purpose we precipitate either with alcohol, which must be evaporated from the filtrate, or by diluting with water, and re- moving the casein by the addition of little acid, and the lactalbumin by coagulation at a boiling heat. The sugar is determined by titration with FEHLING'S or KNAPP'S solution (see Chap. XIV). The principle of titration is the same as for the titration of sugar in urine: 10 c.c. of FEHLI^G'S solution corresponds to 0.067 grm. milk-sugar; 10 c.c. of KNAPP'S solution corresponds to 0.0311-0.0310 grm. milk-sugar, when the saccharine liquid con- tains about ^-1$ sugar. In regard to the modus operandi of the titration we must refer the reader to more complete works and to Chapter XIV. Instead of the volumetric determinations the following steps may be taken: A measured quantity of the milk-sugar solution is treated with an excess of FEELING'S solution, boiled, the copper suboxide filtered and reduced in a current of hydrogen, and the metallic copper weighed. SOXHLET has given a table (Journal fiir praktische Chemie, 1880) which simplifies the calculations in such cases. The sugar may also be determined by the polariscope, and with ease, because the filtrates containing milk-sugar are generally color- less. The determination is quickly performed but does not give exact results. The quantitative composition of cow's milk is variable, The average obtained by KONIG is as follows in 1000 parts : Water. Solids. Casein. Albumin. Pats. Sugar. Salt. 874.2 125.8 28.8 5.3 36.5 48.1 7.1 34.1 The quantity of mineral bodies in 1000 parts of cow's milk is, according to the analyses of SOLDNEE, as follows : K20 1.72, Na20 0.51, CaO 1.98, MgO 0.20, P205 1.82 (after correction for the nucleins), Cl 0.98 grms. BUNGE found 0.0035 grm. Fe203. Ac- cording to SOLDNER, the K, Na and Cl are found in the same quan- tities in whole milk as in milk-serum. Of the total phosphoric acid 36-56$ is not dissolved and also 53-72$ of the lime. A part of this lime is combined with the casein; the remainder is found MILK. 311 united with the phosphoric acid as a mixture of di- and tri-calcium phosphate, which is kept dissolved or suspended by the casein. The bases are in excess of the mineral acids in the milk-serum. The excess of the first is combined with organic acids which correspond to 2.5 p. m. citric acid (SOLDNER). The gases of the milk consist chiefly of C08 , besides a little N and traces of 0. PFLUGER found 10 vols. per cent C02 and 0.6 vol. per cent N, calculated at 0° C. and 760 mm. pressure. The variation in the composition of cow's milk depends on several circumstances. The colostrum, or the milk which is secreted before calving and in the first few days after, is yellowish, sometimes alkaline, but often acid, of high specific gravity, 1.046-1.080, and richer in solids than ordinary milk. The colostrum contains, besides fat-globules, an abundance of colostrum-corpuscles — nucleated granular cells 0.005- 0.025 mm. in diameter with abundant fat-grains and fat-globules. The fat of colostrum has a somewhat higher-melting point and is poorer in volatile fatty acids than the fat from ordinary milk (NiLSON"). The quantity of cholesterin and lecithin is generally greater. The most apparent difference between it and ordinary milk is that colostrum coagulates on heating to boiling be- cause of the absolute and relatively greater quantities of globulin and albumin it contains. The quantity of the first of these two albuminous bodies may indeed amount to several per cent (SEBE- LIEN). The composition of colostrum is very variable. KONIG gives as average the following figures in 1000 parts : Water. Solids. Casein. Albumin and Globulin. Fat. Sugar. Salts. 740.5 259.5 46.6 136.2 34.3 26.6 15.8 The properties of milk are changed during lactation and it becomes richer in casein. A change in the amount of fat is also sometimes observed (EMMERLING). Otherwise we often say that the rnilk becomes poorer in fat during lactation and the fat defi- cient in volatile fatty acids. The evening milk is richer in fat than the morning milk (ALEX. MULLER and ETSENSTUCK ; NILSON). The race of the animal also has a great influence on the milk. The influence food exercises upon milk will be discussed in con- nection with the chemistry of the milk secretion. 312 PHYSIOLOGICAL CHEMISTRY. In the following we give the average composition of skimmed milk and certain other preparations of milk (KoNiG). Water. Proteids. Fat. Sugar. Lactic Acid. Salts. Skimmed milk. ... 906.6 81.1 7.4 47.5 ____ 74 Cream ............ 655.1 36.1 267.5 '65.2 ____ 6.1" Buttermilk ...... 902.7 40.6 9.3 37.3 3.4 6.7 Whey ............ 932.4 8.5 2.3 47.0 3.3 6.5 KUMYSS and KEPHIR are obtained as above stated, by the alcoholic and lactic acid fermentation of the milk-sugar, the first from mare's milk and the last from cow's milk. Large quantities of carbon dioxide are formed hereby, and also the albuminous bodies of the milk are partly converted into albumoses and peptones, which increases the digestibility. The quantity of lactic acid in these preparations may be about 10-20 p. m. The quantity of alcohol varies from 10 to 35 p. m. Milk from other Animals. GOAT'S milk has a more yellowish color and another, more specific, odor than cow's milk. The coagulation obtained by acid or rennet is more solid and is harder than that from cow's milk. SHEEP'S milk is similar to goat's milk, but has a higher specific gravity and contains a greater amount of solids. MARE'S milk is alkaline and contains a casein which is not precipitated by acids in lumps or solid masses, but, like the casein from woman's milk, in fine flakes. This casein is only incompletely precipitated by rennet, and it is very similar also in other respects to the casein of human rnilk. According to BIEL, the casein from mare's and cow's milk is the same, and the different behavior of the two varieties of milk is due to different amounts of salts and to a dif- ferent relation between the casein and the albumin. The milk of the ASS is similar to human milk. The milk of CARNIVORA, the bitch and cat, are acid in reaction and very rich in solids. The composition of the milk of these animals varies very much with the composition of the food. To illustrate the composition of the milk of other animals the following figures, the compilation of KONIG, will be given. As the milk of each variety of animal may have a variable composition, these figures may only be con- sidered as examples of the composition of milk of different kinds. Milk of the Water. Solids. Proteids. Fat. Su^ar. Salts. Dog... 754.4 245.6 99.1 95.7 31.9 7.3 Cat.... Goat... Cow.... Horse... Ass Pig.... 816.3 183.7 90.8 33.3 49.1 58 869.1 130.9 36.9 40.9 44.5 8.6 835.0 165.0 57.4 61.4 39.6 6.6 874.2 125.8 34.1 36.5 48.1 7.1 900.6 99.4 18.9 10.9 66.5 3.1 900.0 100.0 21.0 13.0 630 30 823.7 167.3 60.9 64.4 40.4 1P.6 Human Milk. Woman's milk generally differs from cow's milk in having an alkaline reaction and larger fat-globules. Their number, according to BOUCHUT, is in most cases 1-2 millions in 1 c.mm. The specific MILK. 313 gravity of woman's milk is between 1.026 and 1.035, but varies generally between 1.028 and 1.034. This milk in general has a less inclination to turn acid, and it therefore does not coagulate dis- tinctly. The fat from human milk has not been thoroughly investigated. According to HOPPE-SEYLER, it is richer in fluid fats than the fat from cow's milk. The essential qualitative difference between woman's and cow's milk seems to lie in the proteids, or in the more accurately deter- mined casein. A number of investigators, such as BERZELIUS, SIMON, BIEDERT, LANGGARD, MAKRIS, and others, claim that the casein from woman's milk has other properties than that from cow's milk. The essential differences are the following : The casein from woman's milk is precipitated with greater difficulty with acids or salts ; it does not coagulate regularly in the milk after the addition of rennet ; it may be precipitated by gastric juice, but dissolves completely and easily in an excess of the same; the casein precipitate produced by an acid is more easily soluble in an excess of the acid ; and lastly, the clot formed from the casein does not appear in such large and coarse masses as the casein from cow's milk, but is more loose and flocculent. This last-mentioned fact is of great importance, since it explains the generally-admitted easy digestibility of the casein from woman's milk. The question as to whether the above-mentioned differences depend on a decided difference in the two caseins or only on an unequal relationship be- tween the casein and the salts in the two varieties of milk, or upon other circumstances, has not been sufficiently investigated, and doubtless further experiments will be of great value. We have not, up to the present time, any quite trustworthy analyses of the casein from woman's milk, but it seems probable that the caseins from woman's and cow's milk are not identical albuminous bodies. Besides casein, woman's milk contains lad albumin, and certain investigators maintain that they have found relatively large amounts of albumoses and peptones. According to other state- ments (DOGIEL and HOFMEISTER), no peptones occur in woman's milk, and the methods employed for detecting albumoses seem to have given no positive results. The albuminous bodies of woman's milk require more thorough investigation. The total quantity of 314 PHYSIOLOGICAL CHEMISTRY. milk secreted by both mammary glands amounts to 500-1500 grms. in two hours. The quantitative composition of woman's milk is, even after those differences are eliminated which depend on the imperfect analytical methods employed, variable to such an extent that it is impossible to give any average results. Eliminating certain of the older, incorrect analyses, we here give only examples from the aver- age results of a few modern investigators, taken from a very large number of analyses (PFEIFFEE, LEEDS). The following figures are parts per 1000. Water. Solids. Proteids. Fat, Choles- terin. Sugar. Salts. 876.0 124.0 22.10 23 60 38.10 25 60 0 32 60.90 55 60 2.90 BlEL TOLMATSCHEFF 891 0 109 0 17 90 33 00 53 90 4 20 GERBER 872 4 127 6 19 00 43.20 59.80 2.60 CHRISTENN 892 0 108 0 16 13 32 28 57 94 1 65 20-30 vears old ) -^ 890 6 109 4 17 24 29 15 59 92 2 09 30-40 " " f^FEIFFER 877 90 25 30 38.90 55.40 2 50 MENDES DE LEON 867.32 132.68 19.95 41.31 69.36 2.00 LEEDS Although the composition of woman's milk is very variable, and notwithstanding that in a few cases higher results (about 40 p. m.) have been obtained, by later analyses, for albuminous bodies, still it seems that woman's milk. in general contains less proteids and more sugar than cow's milk. The quantity of casein is not only absolutely but also relatively smaller in proportion to the quantity of albumin in woman's than in cow's milk. A further difference between woman's and cow's milk is that the first is richer in lecithin but poorer in mineral bodies, especially OaO and P206 (it contains onty -J- and J, respectively, of the corre- sponding quantity of these mineral bodies in cow's milk). In regard to the quantity of mineral bodies in woman's milk the analyses of BUNGE are most reliable. He analyzed the milk of a woman, fourteen days after delivery, whose diet contained very little common salt for four days previous to the analysis (A), and again three days later after a daily addition of 30 grms. NaCl to the MILK. 315 food (B). BUNGE found the following figures in 1000 parts of the milk : A B K30 0.780 0.703 NaaO 0.232 0.257 CaO 0.328 0.343 MgO 0.064 0.065 Fe3O8 0.004 0.006 Pa06 0.473 0.469 Cl 0.438 0.445 The relationship of the two bodies, potassium and sodium, to each other may, according to BUNGE, vary considerably (1.3-4.4 equivalents potash to 1 of soda). By the addition of salt to the food the quantity of sodium and chlorine in the milk increases, while the quantity of potassium decreases. The gases of woman's milk have not been investigated. The proper treatment of cow's milk by diluting with water and by certain additions in order to render it a proper substitute for woman's milk in the nourishment of babes cannot be determined before the difference in the albuminous bodies of these two kinds of milk has been completely studied. The period of lactation acts essentially the same on woman's as on cow's milk. The colustrum has a higher specific gravity, 1.040-1.060, a greater quantity of coagulable proteids, and a deeper yellow color than ordinary woman's milk. Even a few days after delivery the color becomes less yellow, the quantity of albumin less, and the number of colostrum-corpuscles diminishes. CLEMM has analyzed the colostrum at different periods before and after delivery, and the following are his results in parts per 1000 : Four Weeks before Delivery. Seventeen Days before Delivery. Nine Days before Delivery. Twenty- four Hours after Delivery. Two Days after Delivery. 1 2 Water 945.2 54.8 852.0 148.0 851.7 148.3 858.8 141.2 843.0 157.0 867.9 132.1 21.8 Solids Albumin 28.8 7.3 17.3 4.4 69.0 41.3 39.5 4.4 74.8 30.2 43.7 4.5 80.7 23.5 36.4 5.4 Fat 48.6 61.0 ^tilk-siiffar . Salts 51 316 PHYSIOLOGICAL CHEMISTRY. The total quantity of albumins seems to decrease with the dura- tion of lactation. PFEIFFER found the average figures for the total proteids for the two first days, the first week, the second week, the second month, and the seventh month to be 86.04, 34.42, 22.88, 18.43, and 15.21 p. m., respectively. SIMON claims that the amount of casein is smaller in the first stages of lactation and then increases considerably; but according to PFEIFFER, just the reverse takes place. The amount of fat shows no regular and constant variation during lactation. According to VERKOIS and BECQUEREL, the quantity of milk-sugar decreases in the first months, but increases in the eighth to the tenth month. According to PFEIFFER, the quantity of sugar increases from the delivery to the third to fourth month, and then it is somewhat variable. The two mammary glands of the same woman may yield somewhat different milk, as shown by SOURDAT and later by BRUNNER. Also the different portions of milk from the same milking may have different composi- tions. The first portions are always poorer in fat (PARMENTIER, PELIGOT, and others). According to L'HERITIER, VERNOIS, and BECQUEREL, the milk of blonds contains less casein than that of brunettes, a difference which TOLMATSCHEFF could not substantiate. Women of weak constitutions yield a milk richer in solids, especially in casein, than women with strong constitutions (V. and B.). According to VERNOIS and BECQUEREL, the age of the woman has an effect on the composition of the milk, so that we find a greater quantity of proteids and fat in women 15-20 years old and a smaller quantity of sugar. The smallest quantity of proteids and the greatest quantity of sugar are found at 20 or from 25-30 years of age. According to V. and B., the milk with the first-born is richer in water — with a proportionate diminution of the quantity of casein, sugar, and fat — than after several deliveries. The influence of menstruation seems to slightly diminish the milk-sugar and to considerably increase the fat and casein (V. and B.). Witch's Milk is the secretion of the mammary glands of new-born children of both sexes immediately after birth. This secretion has from a^qualitative standpoint the same constitution as milk, but may show important differences and variations from a quantitative point of view. SCHLOSSBERGER and HAUFF, GUBLER and QUEVENNE, and v. GESNER have made analyses of this milk and give the following results : 10.5-28 p. m. proteids, 8.2-14.6 p. m. fat, and 9-60 p. m. sugar. As milk is the only form of nourishment during a certain period of the life of man and mammalia, it must contain all the nutritious bodies necessary for life. This fact is shown by the milk-contain- ing representives of the three chief groups of organic-nutritive substances, proteids, carbohydrates, and fat; and all milk seems to contain also some lecithin. The mineral bodies in milk must MILK. 317 also occur in proper proportion, and on this point the observations of BUNGE on dogs are of special interest. He found that the mineral bodies of the milk occur in about the same relative propor- tion as they do in the body of the sucking animal. BUNGE found in 1000 parts of the ash the following results (A represents results from the new-born dog and B the milk from the bitch) : A B KaO 114.2 149.8 Na2O 106.4 88.0 CaO 295.2 272.4 MgO 18.2 15.4 FeaO3 7.2 1.2 P3O6 394.2 342.2 Cl 83.5 169.0 BUNGE explains the fact that the milk-ash is richer in potash and poorer in soda than the new-born animal by saying that in the growing animal the growing muscles rich in potash relatively increase find the cartilage rich in soda relatively decreases. BUNGE seeks to explain the high amount of chlorine in the milk-ash also teleologically by the statement that the chlorides not only serve to build up the tissues, but are indispensable in the secretions of the kidneys. In regard to the amount of iron we find an unexpected condition, the ash of the new-born animal containing six times as much as the milk-ash. This condition BUNGE explains by the fact founded on his and ZALESKY'S experiments, that the quantity of iron in the total organism is highest at birth. The new-born animal has therefore a storage of iron for the growth of its organs even at its birth. The influence of the food on the composition of the milk is of interest from many points of view and has been the subject of many investigations. From these investigations we learn that in human beings as well as in animals an insufficient diet decreases the quantity of milk, and the quantity of solids in the same, while abundant food increases both. From the observations of DECAISITE on nursing women during the siege of Paris in 1871, the quantity of casein, fat, sugar, and salts, but especially the fat, was found to decrease with insufficient food, while the quantity of lactalbumin was found to be somewhat increased. Food rich in proteids in- creases the quantity of milk, and also the solids contained, especially 318 PHYSIOLOGICAL CHEMISTRY. the fat (as shown in women by ZALESKY, in sheep by STOLZMANN, WEISKE, SCHRODT and DEHMEL and MUNK, in dogs by POGGIALE and SSUBBOTIN, in cows — at least in most cases — by KUHN and his pupils). An increase in the quantity of casein, and a decrease in the albumin and the sugar in cow's milk after food containing an excess of proteids has been observed by KUHIT and his pupils. The quan- tity of sugar in woman's milk is found by certain investigators to be increased after food rich in proteids, while others claim it is dimin- ished. Food rich in fat may in sheep, as observed by STOLZMANK WEISKE, SCHRODT and DEHMEL, cause an increase in the quantity of fat in the milk. An increase in the quantity of fat in cow's milk be- cause of an addition of fat to the fodder has only been observed after a previous insufficient diet, but not after a sufficient and rich diet (KiiHtf and FLEISCHMAFK). After feeding with palm-oil cake a one-sided increase in the fat of cow's milk was observed by KUHK. The presence of large quantities of carbohydrates in the food seems to cause no constant, direct action on the quantity of the milk-con- stituents. In carnivora the secretion of milk-sugar proceeds unin- terrupted on a diet consisting exclusively of lean meat. Watery food gives a milk containing an excess of water of little value. In the milk from cows which were fed on distillers' grains COMMAILLE found 906.5 p.m. water, 26.4 p.m. casein, 4.3 p.m. albumin, 18.2 p. m. fat, and 33.8 p.m. sugar. Such milk has a peculiar sour, sharp after-taste. Chemistry of the Milk- Secretion. That the actually-dissolved constituents occurring in milk pass into the secretion, not alone by filtration or diffusion, but more likely are secreted by a specific secretory activity of the glandular elements, is shown by the fact that milk sugar, which is not found in the blood, is to all appear- ances formed in the glands themselves. A further proof lies in the fact that the lactalbumin is not identical with serum-albumin (SEBELIE^ ) ; and lastly, as BUKGE has shown, the mineral bodies secreted by the milk are in quite different proportions than to those in the blood-serum. Little is known in regard to the formation and secretion of the specific constituents of milk. The older theory, that the casein was produced from the lactalbumin by the action of an enzyme, is incor- rect and originated probably from mistaking an alkali-albuminate MILK. 319 for casein. Better founded is the statement that the casein origi- nates from the protoplasm of the gland cells, which seem to consist of casein or a substance related to it. The previously (page 300) mentioned nucleoalbumin of the gland-cells appears to be related to casein, and it may possibly form its mother substance. There does not seem to be any doubt that the protoplasm of the cells takes part in the secretion in such a manner that it becomes itself a constituent of the secretion. According to HEIDENHAIN, the alveoles contain a simple layer of cells, which, in the inactive gland, are flat, polyhedrons, and with single nucleus, while in the active gland they often have several neuclei, are rich in albumin, and are high and cylindrical in form. In the inner part of the cell turned towards the cavity of the acinus, single fat-granules are formed during the secretion which are broken off with the edge of the cells. The broken-off or destroyed cell-substance in the secretion dissolves in the milk, filling the lumen of the acinus, while the cells take up nutrition by their outer parts, and grow, and replace the inner parts used in the secretion. This reminds us of the action of the pan- creas-cells in the secretion of the pancreatic juice. The colostrum- corpuscles are not, according to HEIDENHAIN, degenerated fat- cells, but are contractile elements originating from the epithelium, which take up finely-divided fat and thereby obtain their quantity of fat-globules. That the milk-fat is produced by a formation of fat in the proto- plasm, and that the fat-globules are set free by their destruction, is a generally-admitted opinion which, however, does not exclude the possibility that the fat is in part taken up by the glands from the blood and eliminated with its secretion. A formation of fat from carbohydrates in the animal organism is at the present day consid- ered as positively proved, and it is also possible that the milk-glands also produce fats from the carbohydrates brought to them by the blood. It is a well-known fact that an animal gives off for a long time, daily, considerably more fat in the milk than it receives as food, and this proves that at least a part of the fat secreted by the milk is produced from proteids or carbohydrates, or perhaps from both. The question as to how far this fat is produced directly in the milk-glands, or from other organs and tissues, and brought to the gland by means of the blood, cannot be decided. 320 PHYSIOLOGICAL CHEMISTRY. The origin of the milk-sugar is not known. MUNTZ calls atten- tion to the fact that a quantity of very widely-diffused bodies in the vegetable kingdom — vegetable mucus, gums, pectin bodies — yield galactose as products of decomposition, and he believes, there- fore, that the milk-sugar may be formed in herbivora by a synthesis from dextrose and galactose. This origin of milk-sugar does not answer for carnivora, as they produce milk-sugar when fed on food consisting entirely of lean meat. The observations of BERT and THIERFELDER that a mother-substance of the milk-sugar, a saccha- rogen, occurs in the glands cannot, as the nature of this mother-sub- stance is still unknown, give further explanation as to the formation of milk-sugar. The question whether the above (page 300) mentioned proteid, which yields a reducible substance when boiled with dilute acids, has anything to do with the formation of milk-sugar cannot be answered until further thorough investigations have been made on this subject. The passage of foreign substances into the milk stands in close connection with the chemical processes of the milk-secretion. It is a well-known fact that milk acquires a foreign taste from the food of the animal, which is in itself a proof that foreign bodies pass into the milk. This fact becomes of special importance in reference to such injurious substances as may be introduced into the organism of the nursing child by means of the milk. Among these substances may be mentioned opium and mor- phine, which after large doses pass into the milk and act on the child. Alcohol may pass into the milk in such large quantities that it produces a stupefying effect on the child. Milk from cattle fed on distiller's grains may also contain alcohol. Among the inorganic bodies we find iodine, arsenic, bismuth, antimony, zinc, lead, mercury, and iron in the milk. After inunc- tion-cures PASCHKIS and VAJDA detected mercury in the milk. In icterus neither the bile-acids nor bile-pigments pass into the milk. Under diseased conditions no constant change has been found in woman's milk. In isolated cases SCHLOSSBERGBR, JOLY and FILHOL have observed indeed an essential declining composition, but no positive conclusion can be derived therefrom. The changes in cow's milk have also been little studied. In tuberculosis of the udder STORCH found tubercule bacilli in the milk, and he also found that the milk became more and more diluted during the disease with a serous liquid MILK 321 similar to blood-serum, so that the glands, instead of yielding milk, only gave b cod-serum or a serous fluid. HUSSON found the milk from cows sick with murrain contained more proteids but considerably less fat and (in difficult cases) less sugar than normal milk. The milk may be blue or red in color, due to the development of micro- organisms. The formation of concrements in the exit-passages of the cow's udder are often observed. They consist chiefly of calcium carbonate, or of carbonate and phosphate with only a small amount of organic substances. CHAPTER XIII. THE SKIN AND ITS SECRETIONS. IN" the sliructure of the skin of man and vertebrates many different kinds of substances occur which have already been treated of, such as the constituents of the epidermis formation, the connective and fatty tissues, the nerves, muscles, etc. Among these the different horn-formations, the hair, nails, etc., whose chief constituent, keratin, has been spoken of in another chapter (Chap. II), are of special interest. The cells of the horny formation show, in proportion to their age, a different resistance to chemical reagents, especially fixed alkalies. The younger the horn-cell the less resistance it has to the action of alkalies; with advancing age the resistance becomes greater, and the cell-membranes of many horn-formations are nearly insoluble in caustic alkalies. Keratin occurs in the horn formation mixed with other bodies, from which it is isolated with difficulty. Among these bodies the mineral constituents in many cases occupy a prominent place because of their quantity. Hair leaves on burning 5-70 p. m. ash which contains in 1000 parts 230 parts alkali sulphates, 140 parts calcium sulphate, 100 parts iron oxide, and 400 parts silicic acid. Dark hair seems generally, but not always, to yield more iron oxide on burning than blond. The nails are rich in calcium phosphate, and the feathers rich in silicic acid. The skin of invertebrates has been the subject, in a few cases, of chemical investigation, and in these animals several substances have been found, of which a couple, though little studied, are worth discussing. Among these bodies tumcin, which is found especially in the tunic of the tunicata, and the widely-diffused THE SKIN AND ITS SECRETIONS. 323 chitin, found in the cuticle-formation of invertebrates, are of interest. Tunicin, or animal cellulose, occurs, as above mentioned, in the tunicata. According to BERTHELOT, tunicin differs from ordinary cellulose, being colored yellow by iodine, and by its slower conversion into sugar on boiling with acids. Otherwise it is similar to ordinary cellulose. The sugar obtained by boiling tunicin with acids is grape sugar, according to FRANCHI- MONT. Chitin is not found in vertebrates. The horny layer which covers the inner side of the gizzard of birds, may perhaps consist of a substance related to chitin. In invertebrates the chitin occurs in many classes of animals ; it cannot be positively asserted that true, typical chitin is found elsewhere than in articu- lated animals ; in these it forms the chief organic constituent of the shell, etc. According to SUNDWIK, the composition of chitin is probably CaoHiooN8OS8 4- w(H3O), where n may vary from 1 to 4. On boiling with mineral acids it decomposes and yields, as LEDDERHOSE has shown, glucosamine. According to SUNDWIK, a glucose is also probably hereby produced. He also claims that chitiu is an aniido derivative of a carbohydrate of the formula C8oHi0oO6o. In the dry state chitin forms a white, brittle mass retaining the form of the original tissue. It is insoluble in boiling water, alcohol, ether, acetic acid, dilute mineral acids, and dilute alkalies. It is soluble in concentrated acids. It is dissolved without decomposing in cold concentrated hydrochloric acid, but is decomposed by, boiling hydrochloric acid. When chitin is dissolved in concentrated sulphuric acid and the solution dropped into boiling water and then boiled, we obtain a, substance (glucosamine or glucose) which reduces copper suboxide in alkaline solutions. Chitin may be easily prepared from the wings of insects or from the shells of the lobster or the crab, the last mentioned having first been extracted by an acid so as to remove the lime-salts. The wings or shells are boiled with caustic alkali until they are white, afterward washed with water, then with dilute acid and water, and lastly extracted with alcohol and ether. Hyalin is the chief organic constituent of the walls of hydatid cysts. From a chemical point of view it stands close to chitin, or between it and the albumins. In old and more transparent sacks it is tolerably free from mineral bodies, but in younger sacks it contains a great quantity (16#) of lime-salts (carbonate, phosphate, and sulphate). According to LUCRE, its composition is : C H N O From old cysts 45.3 6.5 5.2 43.0 From young cysts 44.1 6.7 4.5 44.7 It differs from keratin on the one hand and from albumin on the other by the absence of sulphur, also by its yielding a variety of sugar in large quantities (50$), which is reducible, fermentable, and dextro-gyrate when boiled with dilute sulphuric acid. It differs from chitin by the property of being gradually dissolved by caustic potash or soda, or by dilute acids ; also by its solubility on heating with water to 150° C. The coloring matters of the skin and horn-formations are of different kinds, but have been but little studied. Those occurring in the Malpighian layer of the skin, especially of the negro, and 324 PHYSIOLOGICAL CHEMISTRY. the black or brown pigment occurring in the hair oelong to the group of coloring matters which have received the name melanin. Melanins. This group includes several different varieties of amorphous black or brown pigments which are insoluble in water, alcohol, ether, chloroform, and dilute acids, and which occur in the skin, hair, epithelium -cells of the retina, in sepia, in certain pathological formations, and in the blood and urine in disease. Of these pigments there are a few, such as the melanin of the eye and that from the melanotic tumor of horses, the hippomelanin (NENCKI and BERDEZ), which are soluble with difficulty in alkalies, while others, such as the pigment of the hair and the coloring matter of certain pathological swellings in man, the pliymatorusin (NENCKI and BERDEZ), are easily soluble in alkalies. Among the melanins there are a few, for example the choroid pigment, which are free from sulphur; others, on the contrary, as the pigment of the hair and of horse-hair, are rather rich in sul- phur (2-4$), while the phymatorusin found in certain swellings and in the hair (NENCKI and BERDEZ, K. MORNER) is very rich in sulphur (8-10$). If any of these pigments, especially the phyma- torusin, contains any iron or not is an important though disputed point, for it leads to the question whether these pigments are formed from the blood-coloring matters (NENCKI and SIEBER, K. MOR- NER). The difficulties which attend the isolation and purification of the melanins have not been overcome in certain cases, while in others it is questionable whether the final product obtained has not another composition than the original coloring matter, owing to the energetic chemical processes resorted to in its purification. Under such circumstances it seems that a tabulation of the analy- ses of different melanin preparations made up to the present time are of secondary importance. Among the above-mentioned bodies belonging to the melanin group, phymatorosin prepared by NENCKI and SIEBER from mel- anotic tumors, and by K. MORNER from the tumors and" the urine of a patient, seems to be of special interest. Phymatorusin is an amorphous dark-brown coloring matter soluble in alkalies or alkali carbonates, but insoluble in warm 50-75$ acetic acid. In alkaline solution it shows no absorption-bands. According to NENCKI and SIEBER, it is free from iron, but MORJJER, on the contrary, claims THE SKIN AND ITS SECRETIONS. 325 that it does contain iron. MORNER found for this coloring mat- ter from tumors (A) and from urine (B) the following composition calculated on the substance considered as ash-free : A B C 55.32—56.13 55.76 H 5.65— 6.33 5.95 N 12.30 12.27 S 7.97 9.01 Fe 0.063-0.081 0.20 NENCKI and SIEBER have also shown that other melanins, not identical with phymatorusin, occur in melanotic sarcoma of man. The coloring matter or matters of human hair contain a low amount of nitrogen, 8.5$ (SIEBER), and a variable but high amount of sulphur, 2.71-4.10$. The considerable quantity of iron oxide found in the ash does not seem to belong to the coloring matters. In addition to the coloring matters of the human skin we may also here treat of the pigments found in the skin or epidermis-formation of animals. The beautiful color of the feathers of many birds depends in certain cases on purely physical causes (interference-phenomena), but hi other cases on col- oring matters of various kinds. Such a coloring matter is the amorphous red- dish-violet turacin, which contains copper, and whose spectrum is very similar to that of oxyhsemoglobin. KRUKENBERG found a large number of coloring matters in birds' feathers, namely, zooerythrin, zoofulvin, turacoverdin, zoom- bin, psittacofulmn, and others which cannot be enumerated here. Tetronerythrin, so named by WURM, is a red amorphous coloring matter which is soluble in alcohol and ether, and which occurs in the red warty spots over the eyes of the heath-cock and the grouse, and which is very widely spread among the invertebrates (HALLIBURTON, DE MEREJKOWSKI, MACMUNN). Besides tetronerythrin MACMUNN found in the shells of crabs and lobsters a blue coloring matter, cyanocrystallin, which turns red with acids and by boiling water. Hcematoporphyrin, according to MACMUNN, also occurs in the integu- ments of certain lower animals. In addition to the coloring matters thus far mentioned a few others found in certain animals (though not in the skin) will be spoken of. Carminic acid, or the red coloring matter of cochineal, has the composition CnHjeOio. It gives sugar on boiling with acids, but this does not correspond -with the recent statements of LIEBERMANN. The beautiful purple solution of ammonium carmiuate has two absorption-bands between D and E which are similar to those of oxyhaemoglobin. These bands lie nearer to E and closer together and are less sharply defined. Purple is the evaporated residue from the purple- violet secretion, caused by the action of the sunlight, from the so- called " purple gland " of the tunic of certain species of murex and purpura. Its chemical nature has not been investigated. Among the remaining coloring matters found in invertebrata we may men- tion blue stentorin, actiniodirom, bonellin, polyperythrin, peniacrinin, ante- donin, cru8taceorubin,janthinin, and chlorophyll. 326 PHYSIOLOGICAL CHEMISTRY. Sebum, when freshly secreted, is an oily semi-fluid mass which solidifies on the upper surface of the skin, forming a greasy coating. The quantity is very different in different persons. HOPPE-SEYLER has found a body similar to casein, besides albumin and fat, in the sebum. Cholesterin is also found in this fat, and in especially large quantities in the vernix caseosa. The solids of the sebum consist chiefly of fat epithelium-cells and protein bodies; the vernix case- osa consists chiefly of fat. Cerumen is a mixture of the secretion of the sebaceous and sweat glands of the cartilaginous part of the outer organs of hear- ing. It contains chiefly soaps and fat, and besides these a red sub- stance easily soluble in alcohol and with a sweetish-bitter taste. The preputial secretion, smegma praputii, contains chiefly fat, also cholesterin and ammonium soaps, which probably are produced from decomposed urine. The hippuric acid, benzoic acid, and cal- cium oxalate found in the smegma of the horse have probably the same origin. We may also consider as a preputial secretion the castoreum, which is secreted by two peculiar glandular sacks in the prepuce of the beaver. Thi& castoreum is a mixture of proteids, fat, resins, traces of phenol (volatile oil), and a non-nitrogenized body, castorin, crystallizing in four-sided needles from alcohol, insoluble in cold water, but somewhat soluble in boiling water, and whose composition is little known. Wool-fat, or the so-called fat-sweat of sheep, is a mixture of the secretion of the sudoriparous and sebaceous glands. We find in the watery extract a large quantity of potassium which is combined with organic acid, volatile and non- volatile fatty acids, benzoic acid, phenol-sulphuric acid, lactic acid, malic acid, succiuic acid, and others (BuisiNE). The fat contains among other bodies abundant quantitiesof ethers of fatty acids with cholesterin and isocholesteriu. WEBER found that from the glands of the skin of a kangaroo and a dwarf- antelope a colorless secretion was eliminated which in the first case, when ex- posed to the air, formed a red and in the second case a blue coloring matter. The secretion of the coccygeal glands of ducks and geese contains a body sim- ilar to casein, besides albumin, nuclein, lecithin, and fat, but no sugar CDs JONGE). Poisonous bodies have been found in the secretion of the skin of the salamander and the toad, respectively, samandarin (ZALESKY) and bufidin(Jon~ NARA and CASALI). The Sweat. A disproportionally large part of the secretions of the skin whose quantity amounts to about -fa of the weight of the body consists of water. Next to the kidneys the skin is the most important means for the elimination of water. As the glands of the skin and the kidneys stand near to each other in regard to THE SKIN AND ITS SECRETIONS. 327 their functions, they may to a certain extent act vicariously for one another. Various conditions affect both the character and quantity of the sweat secreted. The secretion differs for different parts of the skin, and it has been stated that the perspirations of the cheek the palm of the hand, and under the arm stand to each other as 100 : 90 : 45. From the unequal secretion on different parts of the body it follows that no results as to the amount of secretion for the en- tire surface of the body can be calculated from the amount secreted by a small part of the skin in a given time. In determining the total amount a stronger secretion is as a rule produced, and as the glands can with difficulty work for a long time with the same energy, it is hardly correct to estimate the quantity of secretion per 24 hours from a strong secretion enduring only a short time. FAVRE obtained 2560 grms. sweat in 1£ hours from the entire surface of the body in a steam-bath and with abundant drinking of water. The perspiration obtained for investigation is never quite pure, but contains cast-off epidermis-cells, also cells and fat-globules from the sebaceous glands. Filtered sweat is a clear, colorless fluid with a salty taste and of different odors from different parts of the body. The physiological reaction is acid, according to most statements ; still after continuous secretion the sweat may be alka- line (FAVRE and GILLIBERT, TRUMP Y and LUCHSINGER). An alkaline reaction may also depend on a decomposition with the formation of ammonia. According to a few investigators, the phy- siological reaction is alkaline, and an acid reaction depends, accord- ing to these investigators, upon an admixture of fatty acids from the sebum. MORIGGIA found that the sweat from herbivora was ordinarily alkaline, while that from carnivora was generally acid. The specific gravity of sweat is 1.003-1.005. Perspiration contains 977.4-995.6 p. m., average 988.2 p. m., water, and 4.4-22.6 p. m., average 11.80 p. m., solids. The organic bodies are neutral fats, cholesterin, volatile fatty acids, traces of albumin (according to LECLERC habitually in horses ; sometimes in man after hot baths, in BRIGHT'S disease, and after the use of pilocarpin), also creatinin (CAPRANICA), aromatic oxyacids, ethereal sulphuric acids of phenol and skatoxyl (KAST), but not of indoxyl, and lastly urea. The amount of urea, which according to FUNKE 328 PHYSIOLOGICAL CHEMISTRY. is 1.99 p. m., has been more exactly determined in later times by ARGUTINSKY. In two steam-bath experiments, in which in the course of -J and f hour he obtained respectively 225 and 330 c. c. sweat, he found 1.61 and 1.24 p. m. urea. In uraemia, and in ischuria in cholera, urea may be secreted in such quantities by the sweat-glands that crystals deposit upon the skin. The mineral bodies consist chiefly of sodium chloride with some potassium chloride, alkali sulphate, and phosphate. The relative amounts of these in perspiration differ materially from the amounts in the urine (FAYEE, KAST). The relationship, according to KAST, is as follows : Chlorine : Phosphate : Sulphate In perspiration 1 : 0.0015 : 0.009 In urine 1 : 0.1320 : 0.397 KAST found that the proportion of ethereal sulphuric acid to the sulphate sulphuric in sweat was 1 : 12. After the administra- tion of aromatic substances the ethereal sulphuric acid does not increase to the same extent in the sweat as in the urine (see Chap. XIV). Sugar may pass into the sweat in diabetes, but the passage of the bile-col- oring matters has not been positively shown in this secretion. Benzoic acid, succinic acid, tartaric acid, iodine, arsenic, mercuric chloride, and quinine pass into the sweat. Uric acid has also been found iii the sweat in gout, and cystin in cystinura. Chromhidrosis has been called the secretion of colored sweat. Sometimes sweat has been observed to be colored blue by indigo (Bizio), by pyocyanin (FoRDOs), or by ferro-phpsphate (KOLLMANN). True blood-sweat, in which blood- corpuscles exude from the openings of the glands, has also been ob- served. The exchange of gas through the skin in man is of very little importance compared to the exchange of gas by the lungs. The absorption of oxygen by the skin, which was first shown by REG- NAULT and REISET, is very small. The quantity of carbon dioxide eliminated by the skin increases with the rise of temperature (AUBERT, ROHRIG, FuBiia, and RONCHI). It is also greater in light than in darkne'ss. It is greater during digestion than when fasting, and greater after a vegetable than after an animal diet (FuBiia and RotfCHi). According to SCHARLING it is 10 grins., and according to AUBEKT 3.9 grms., in 24 hours. In certain ani- THE SKIN AND ITS SECRETIONS. 329 mals, as in frogs, the exchange of gas through the skin is of great importance. As the exchange of gas through the skin in man and mammalia is very small, it follows that the injurious and dangerous effects caused by covering the skin with varnish, oil, or the like, can hardly depend on a prevented exchange of gas. After varnishing the skin there is a considerable loss of heat, and the animal quickly dies. If the animal, on the contrary, be guarded from this loss of heat, it may be saved, or kept at least alive, for a longer time. This effect was supposed to be due to a poisoning caused by a retention of one or more substances of the perspiration (perspirabile retentum), ac- companied by fever and increased loss of heat through the skin; but this statement has not been substantiated. This phenomenon seems to be due to other causes, and at least in certain animals (rabbits) death seems to ensue from the paralyzation of the vaso- motor nerves. In anastomosis the loss of heat through the skin seems to be increased to such an extent that the animal dies from the lowered temperature. TJHITBBSITY CHAPTER XIV. THE URINE. THE urine is the most important excretion of the animal or- ganism; it is the means of eliminating the nitrogenized products of exchange, also the water and the soluble mineral substances; and in many cases it furnishes important data relative to the exchange of material ; quantitatively, by its variation, and qualita- tively by the appearance of foreign bodies in the excretion. Also in many cases we are able from the chemical or morphological constituents which the urine abstracts from the kidneys, ureters, bladder, and urethra to judge of the condition of these organs; and lastly, urinary analysis affords an excellent means of deciding the question how certain medicines or other foreign substances introduced into the organism are absorbed and chemically changed. Urinary analysis has furnished very important particulars espe- cially relative to the last-mentioned question in regard to the nature of the chemical processes taking place within the organism, and it is therefore not only an important aid in diagnosis to the physician, but it is also of the greatest importance to the toxicol- ogist and the physiological chemist. In studying the secretions and excretions the relationship must be sought between the chemical structure of the secreting organ and the chemical composition of its secreted products. Investiga- tions with respect to the kidneys and the urine have led to very few results from this standpoint. Although the anatomical rela- tion of the kidneys has been carefully studied, their chemical com- position has not been the subject of thorough analytical research. In cases in which a chemical investigation of the kidneys has been undertaken, it has only been in general on the organ as such, and not on the different anatomical parts. An enumeration of the 330 THE URINE. 331 chemical constituents of the kidneys known at the present time can, therefore, only have a secondary value. In the kidneys we find albuminous bodies of different kinds, namely, globulin, albumin, and nucleoalbumin, also a gelatine- forming and elastic substance, and lastly a body similar to mucin. The question as to whether pure mucin really exists in the kidneys has not been decided. The body similar to mucin, which is a nucleoalbumin and which gives no reducible substance when boiled with acids (LONNBERG), belongs chiefly to the papillae, while the nucleoalbumin dissimilar to mucin belongs to the cortical sub- stance. Fat only occurs in very small amounts in the cells of the tortuous urinary passages. Among the extractive bodies of the kidneys we find xanthin bodies, also adenin (KRONECKER), urea, and traces of uric acid, glycogen, leucin, inosit, taurin, and cystin (in ox-kidneys). The quantitative analyses of the kidneys thus far made possess little interest. The quantity of water in human kid- neys is 828.4 p. m. according to FRERICHS, and 834.5 p. m. according tO VOLKMANN. The fluid collected under pathological conditions, as in hydrouephrosis, is thin with a variable but generally low specific gravity. Usually it is straw- yellow or paler in color, and sometimes colorless. Most frequently it is clear, or only faintly cloudy from white blood-corpuscles and epithelium-cells ; in a few cases it is so rich in form-elements that it appears like pus. Albumin occurs generally only in small amounts ; sometimes it is entirely absent, and in a few rare cases the amount is nearly as large as in the blood-serum. Urea occurs sometimes in considerable amounts when the parenchyma of the kidneys is only in part atrophied ; in complete atrophy the urea may be entirely absent. I. Physical Properties of Urine. Consistency, transparency, odor, and taste of urine. Urine is under physiological conditions a thin liquid and gives, when shaken with air, a froth which quickly subsides. Human urine or urine from carnivora, which is habitually acid, appears clear and transparent, often faintly fluorescent immediately after voiding. When allowed to stand for a little while human urine shows a light cloud (nubeculd) which consists of the so-called "mucus" and generally also contains a few epithelium-cells, mucus-corpuscles, and urate-granules. The presence of a larger quantity of urates (uric-acid salts) renders the urine cloudy, and a clay-yellow, 332 PHYSIOLOGICAL CHEMISTRY. yellowish-brown, rose-colored, or often brick-red precipitate (sedi- mentum latentium) settles on cooling because of the greater insolu- bility of the urates at the ordinary temperature than at the temper- ature of the body. This cloudiness disappears on gently warming. In new-born infants the cloudiness of the urine during the first 4-5 days is due to epithelium, mucus-corpuscles, uric acid, and urates. The urine of herbivora, which is habitually neutral or alkaline in reaction, is very cloudy on account of the carbonates of the alkaline earths present. Human urine may sometimes be alkaline under physiological conditions. In this case it is made cloudy by the earthy phosphates, and this cloudiness does not dis- appear on warming, differing in this respect from the sedimvntum lateritium. Urine has a salty and faintly-bitter taste produced by sodium chloride and urea. The odor of urine is peculiarly aro- matic; the bodies which produce this odor are unknown. The color of urine is normally pale yellow when the specific gravity is 1.020. The color otherwise depends on the concentration of the urine and varies from pale straw-yellow, when the urine contains small amounts of solids, to a dark reddish yellow or reddish brown in stronger concentration. As a rule, the intensity of the color corresponds to the concentration, but under pathological conditions exceptions occur, and such an exception is found in diabetic urine, which contains a large amount of solids and has a high specific gravity and a pale yellow color. The reaction of urine depends essentially upon the composition of the food. The carnivora void an acid, the herbivora a neutral or alkaline, urine. -If a carnivora is put on a vegetable diet, its urine may become less acid or neutral, while the reverse occurs when an herbivora is starved, that is, when it lives upon its own flesh, as then the urine voided is acid. The urine of a healthy man on a mixed diet has an acid reaction, and the sum of the acid equivalents is greater than the sum of the base equivalents. This depends on the fact that in the physiolog- ical burning of neutral substances (proteids and others) within the organism acids are produced, chiefly sulphuric acid, but also phosphoric and organic acids, such as hippuric, uric, and oxalic acid, also aromatic oxyacids and others. From this it follows that the acid reaction is not due to one acid alone. We do not know to THE URINE. 333 what extent any one acid takes part in the acid reaction; but as the .sum of the base equivalents is greater than, or at least the same as, the sum of the inorganic acid equivalents, the acid reaction must be due in greatest part to organic acids or acid salts. It is generally considered that the acid reaction of human urine is caused by double-acid alkali-phosphate (monophosphate). The amount of acid-reacting bodies or combinations eliminated by the urine in 24 hours, when calculated as oxalic acid or hydrochloric acid, is re- spectively 2-4 and 1.15-2.3 grms. (VOGEL). The composition of the food is not the only influence which affects the degree of acidity of human urine. For example, after taking food, at the beginning of digestion, when a larger amount of gastric juice containing hydrochloric acid is secreted, the urine may be neutral or even alkaline. The greatest amount of acid or acid- salts per hour is secreted, according to VOGEL, during the night, while according to HOFFMANN, it is in the afternoon. The smallest amount is voided, according to HOFFMANN during the night, but QUINCKE claims that it is in the morning. It has not infrequently been observed that perfectly healthy persons in the morning void a neutral or alkaline urine which is cloudy from earthy phosphates. The effect of muscular activity on the acidity of urine is yet undetermined. According to HOFFMANN and EINGSTEDT, mus- cular work raises the degree of acidity, but ADUCCO claims that it decreases it. Abundant perspiration reduces the acidity (HOFF- MANN). In man and carnivora it seems that the degree of acidity of the urine cannot be increased above a certain point, even though mineral acids or organic acids which are burnt up with difficulty are taken in large quantities. When the supply of carbonates of the fixed alkalies stored up in the organism for this purpose is not sufficient to combine with the excess of acid, then ammonia is split from the proteids or their decomposition products, and the excess of acid combines therewith, forming ammonium-salts which pass into the urine. In herbivora this splitting of ammonia and forma- tion of ammonia-salts does not seem to take place, and the herbiv- ora therefore soon die when acids are given. Nevertheless, the degree of acidity of human urine may be easily diminished so that the reaction is neutral or alkaline. This occurs after the taking of 334 PHYSIOLOGICAL CHEMISTRY. carbonates of the fixed alkalies or of such salts of vegetable acids — tartaric acid, citric-acid, and malic-acid salts — as are easily burnt into carbonates in the organism. Under pathological conditions, as in the absorption of alkaline transudations, the urine may become alkaline The degree of acidity cannot be determined by the ordinary acidi- metric process, since the urine contains di-hydrogen phosphate, MH2P04, besides hydrogen di-phosphate M2HP04. In the titra- tion the di-hydrogen phosphate is changed gradually into MgHPO^, and we obtain at a certain point a mixture of the two phosphates in variable proportions, which mixture is not neutral but amphoteric. Since it is generally admitted that the acid reaction of urine is due to the di-hydrogen phosphate, it is therefore best to express the degree of acidity by the amount of di-hydrogen phosphate present. If we wish to calculate the degree of acidity of the urine as di-hydrogen phosphate or, still more simply, as phosphoric anhydride, P205, contained in this salt, the titration is performed according to the method of MALY and HOFFMANN, which is as fol- lows : The urine (100-200 c. c.) is treated with an exactly-measured quantity of £ normal caustic-soda solution, which is more than suf- ficient to convert all the phosphate into basic phosphate, or, in other words, enough to make the urine strongly alkaline. Then an approximate f normal Ba012 solution (142.8 grms. BaCl2,2H03in a litre) is added until no further precipitate is formed. By this means all the phosphoric acid is precipitated from the urine. Filter through a dry filter, measure a quantity corresponding to 50 or 100 c. c. of the original urine from the filtrate, and titrate with J normal sulphuric acid until a neutral reaction is obtained, using litmus-paper as an indicator. If the amount found by this titration be subtracted from the original amount of caustic soda added to this volume of urine, the difference is the amount of caustic soda necessary to convert the existing di-hydrogen and hydrogen di-phos- phates into normal phosphate. If we designate this by a, and the quantity of total P206 in milligrammes in the same quantity of urine, as determined by a method which will be described later, by g, then we obtain the quantity of P205 in milligrammes in the di-hy- drogen phosphate s by the following formula : s = 17.75 a — g (HUPPERT). If, for example, in a case in which the conversion of both phos- phates into normal phosphate in 100 c.c. of the urine required 20 c.c. caustic soda, while the total quantity of P205 in 100 c.c. urine was 275 milligrammes, then : s = 17.75 X 20 — 275 = 80 milligrammes. The quantity of P205 as simple acid phosphate was therefore 195 milligrammes. THE URINE. 335 A urine with an alkaline reaction caused by fixed alkalies has a very different diagnostic value than one whose alkaline reaction is caused by the presence of ammonium carbonate. In the latter case we have to deal with a decomposition of the urea of the urine by the action of micro-organisms. If we wish to determine whether the alkaline reaction of the urine is due to ammonia or fixed alkalies, we dip a piece of red litmus-paper into the urine and allow it to dry exposed to the air or to a gentle heat. If the alkaline reaction is due to ammonia, the paper becomes red again ; but if it is caused by fixed alkalies, it remains blue. The specific gravity of urine, which is dependent upon the relationship existing between the quantity of water secreted and the solid urinary constituents, especially the urea and sodium chloride, may vary considerably, but is generally 1.017-1.020. After drinking large quantities of water it may fall to 1.002, while after profuse perspiration or after drinking very little water it may rise to 1.035-1.040. In new-born infants the specific gravity is low, 1.007-1.005. The determination of the specific gravity is an im- portant means of learning the average amount of solids elimi- nated from the organism with the urine, and on this account the determination becomes of true value only when at the same time the quantity of urine voided in a given time is determined. The dif- ferent portions of urine voided in the course of the 24 hours are collected, mixed together, the total quantity measured, and then the specific gravity taken. The determination of the specific gravity is most accurately ob- tained with the pyknometer. For ordinary cases the specific grav- ity may be determined with sufficient accuracy by means of areometers. The areometers found in the trade, or urinometers, are graduated from 1.000 to 1.040; for exact observations it is better to use two urinometers, one graduated from 1.000 to 1.020, and the other from 1.020 to 1.040. A special urinometer is that of HELLER, which is graduated according to Baume's scale, from 0 to 8. Each degree corresponds to 7 degrees of the ordinary urinometer, and as the zero-point of HELLER'S urinometer corresponds to the figure 1000, then the 1, 1.5, 2, 2.5, 3, etc., degrees of HELLER'S urinometer correspond to 1.007, 1.0105, 1.014, 1.0175, 1.024, etc., of the ordi- nary specific gravity. 336 PHYSIOLOGICAL CHEMISTRY. To determine the specific gravity of urine, if necessary filter the urine, or if it contains a urate sediment, first dissolve it by gentle heat, then pour the clear urine into a dry cylinder, avoiding the formation of froth. Air-bubbles or froth, when present, must be removed with a glass rod or filter-paper. The cylinder, which must be about £ full, must be wide enough to allow the urinometer to swim freely in the liquid without touching the sides. The cylinder and urinometer should both be dry or previously washed with the urine. On reading, the eye is brought on a level with the lower meniscus — which occurs when the surface ,of the liquid and the lower limb of the meniscus coincide ; the* 'reading is then made from the point where this curved line cuts the scale of the urinometer. If the eye is not in the same horizontal plane with the convex line of the meniscus, but is too high or too low, the surface of the liquid assumes the shape of an ellipse, and the reading in this position is incorrect. Before reading press the urinometer gently down into the liquid and then allow it to rise, and wait until it is at rest. If the quantity of urine at disposal is not sufficient to fill the cylinder to the proper height it may be diluted, according to circum- stances, with an equal volume or several volumes of water. This does not give quite accurate results, and with small quantities of urine it is best to determine the specific gravity by means of the pyk- nometer. Each urinometer is graduated for a certain temperature, which is marked on the instrument, or at least on the best. If the urine is not at the proper temperature, the following corrections must be made : For every three degrees above the normal temperature one unit of the last order is added to the reading, and for every three degrees below the normal temperature one unit (as above) is sub- tracted from the specific gravity observed. For example, when a urinometer graduated for -f 15° C. shows a specific gravity of 1.017 at -f 24° C., then the specific gravity at -f 15° 0. = 1.017 + 0.003 = 1.020. II. Organic Physiological Constituents of the Urine. t Urea, Ur, which is ordinarily considered as carbamid, CO(NH2)2, may be synthetically prepared in several different ways, namely, from carbonyl-chloride, or carbonic-acid ethyl-ether and ammonia, COC12 + 2NH3 = CO(NH2)2 + 2HC1, or (C2H5)2.02.CO + 2NH3 = 2(C2H6.OH) -f CO(NH2)2; by the metameric decomposition of ammonium cyanate, (NH,).O.CN = CO(NH2)2 (WOHLER, 1828); and in many other ways. It is also formed by the decomposition THE URINE. 337 or oxidation of certain bodies found in the animal organism, such as creatin and uric acid. Urea is found most abundant in the urine of carnivora and man, but in smaller quantities in that of herbivora. The quantity in human urine is ordinarily 20-30 p. m. It has also been found in the urine of certain birds and amphibians. Urea occurs in the per- spiration in small quantities, about 2 p. m., and as traces in the blood and in most of the animal fluids. It is also found in certain tissues and organs, especially in the liver (in mammalia) and the spleen, but not in the muscles. Under pathological conditions, as in obstructed secretion of urine, the urea in the animal fluids and tissues may increase to a considerable amount. Under these cir- cumstances it may also occur in the muscles. The quantity of urea which is voided in 24 hours on a mixed diet is in a grown man about 30 grammes, for women somewhat less. Children void absolutely less, but relative to their body- weight the excretion is larger than in grown persons. The physio- logical significance of urea lies in the fact that this body forms in man and carnivora, from a quantitative standpoint the most im- portant nitrogenized final product of the metabolism of proteid bodies. On this account the elimination of urea varies to a great extent with the amount of proteid transformed, and above all with the quantity of absorbable proteids in the food taken. The elimina- tion of urea is greatest after an exclusive meat diet, and lowest, indeed less than during starvation, after the consumption of bodies free from nitrogen, for these diminish the metabolization of the proteids of the body. If the consumption of the proteids of the body is increased, then the elimination of urea is correspondingly increased. This is found to a rather high degree in certain diseases with fever; also, though to a less extent, after taking common salt or after abundant drink- ing of water (Vorr). The elimination of urea is also somewhat increased by several medicines; and lastly it is also increased after poisoning with arsenic, antimony, and phosphorus, by the diminished supply of oxygen — as in severe and continuous dyspnoea — poisoning with carbon monoxide, hemorrhage, etc. In these cases an exact difference has not always been made between the urea and the total quantity of nitrogen in the urine, and it is just this last which is 338 PHYSIOLOGICAL CHEMISTRY. often the cause of the increase found in these cases. The amount of nitrogen contained in the urea does not correspond with the total amount of nitrogen in the urine, since, as PFLUGER has shown, the amount of nitrogen which under physiological conditions occurs combined with other combinations than urea is, on an average, 13.4$, sometimes indeed 16$, of the total nitrogen. In disease this relationship may be essentially changed and, as an example, in a case of phosphorus poisoning FRAENKEL observed that the amount of nitrogen contained in the urea was less than one half of the total amount of nitrogen in the urine. A diminished elimination of urea occurs in a reduced consump- tion of proteids, and also — a fact which is of interest in regard to the importance of the liver in the formation of urea — in certain diseases of the liver, as in acute yellow atrophy and sometimes in interstitial cirrhosis. Under these conditions the amount of ammo- nia in the urine as compared to the urea may be increased (HAL- LERVORDEN, STADELMA^N). In diseases of the kidneys which disturb or destroy the integrity of the epithelium of the tortuous urinary passage the elimination of urea is considerably diminished. Formation of urea in the organism. The experiments to produce urea directly from the proteids by oxidation have not led to any positive results. We often obtain amido-acids as decomposition products of albumin (see page 17), and we therefore conclude that the amido-acids are intermediate steps in the formation of urea from albumin. It has also been shown that leucin and glycocoll (SCHULTZEK and NENCKI, SALKOWSKI), and asparaginic acid (v. KNIERIEM) may be transformed into urea within the organism. The nature of the chemical processes by which these transforma- tions are effected is unknown. Many other ways for the formation of urea have been suggested, but the only one which has been demonstrated is the formation from ammonium carbonate in the liver. After the researches of v. KNIERIEM, SALKOWSKI, FEDER, MUKCK, SCHMIEDEBERG and WALTER, and HALLERVORDE^ had shown that ammonium carbonate, or such ammonium-salts as are burnt up in the system into carbonate, is converted into urea in the body of carnivora and herbivora, v. SCHRODER furnished a decisive proof of the formation of urea from ammonium carbonate in the liver of mammalia. By passing blood which had been treated with THE URINE. 339 ammonium carbonate or ammonium formate through a dog's liver, he found that a considerable formation of urea took place. The very careful observations of SALOMON confirm this, and also the above-mentioned decrease of urea and increase of ammonia in the urine in certain diseases of the liver. It cannot be denied that the formation of urea in the liver is of great physiological importance ; but still it does not follow that all the urea has its origin in this organ. The possibility of a formation of urea from creatin or xanthin bodies is not to be rejected without further inquiry, and other possibilities as to its formation are con- ceivable. The question in which organ urea is formed has been the subject of much discussion. From the researches of numerous investigators, PREVOST and DUMAS, MEISSNER, VOIT, GREHANT, GSCHEIDLEN, SALKOWSKI, and v. SCHRODER, it has been found that the extirpation of the kidneys causes a considerable increase in the quantity of urea in the blood. The kidneys, therefore, although they may produce urea generally, are not the only organs which produce it. By experiments performed on the removed kidneys, which were analogous to the above-mentioned experiments on the removed liver, v. SCHRODER has shown that neither the kidneys nor the muscles nor the remaining tissues of the lower extremities of the dog have the property of forming urea from ammonium car- bonate. The only organ in which a formation of urea has been proved with certainty, thus far, is the liver, but this does not ex- clude the possibility that urea may also be formed in other organs, perhaps from other material than ammonium carbonate and in other ways. Properties and Reactions of Urea. Urea crystallizes in needles or in long, colorless, four-sided, often hollow, anhydrous rhombic prisms. It has a neutral reaction and produces a cooling sensation on the tongue like saltpetre. It melts at 130°-132° C., but decom- poses already at about 100° C. At ordinary temperatures it dis- solves in equal weight of water and in five parts alcohol; it requires one part boiling alcohol for solution; it is sparingly soluble in ether. If urea in substance is heated in a test-tube it melts, de- composes, gives off ammonia, and leaves finally a non-transparent white residue which, among other substances, contains biuret and 340 PHYSIOLOGICAL CHEMISTRY. which dissolves in water, giving a beautiful reddish-violet liquid with copper sulphate and alkali (biuret reaction). On heating with baryta-water or caustic alkali, urea splits into carbon dioxide and ammonia with the addition of water ; this also takes place by the action of micro-organisms, which produces the so-called alkaline fermentation of urine. The same decomposition is produced when urea is heated with concentrated sulphuric acid. An alkaline solu- tion of sodium hypobromite decomposes urea into nitrogen, carbon dioxide, and water according to the equation COIST2H4 + SNaOBr = 3NaBr + C02 +2H20 + N2. Urea forms with many acids crystalline combinations. Among these the one with nitric acid and the one with oxalic acid are the most important. UEEA NITRATE, CO(NH2)2.HN03. This combination on crys- tallizing quickly forms thin rhombic or six-sided, overlapping tiles, colorless plates, whose point has an angle of 82°. On slowly crystallizing, larger and thicker rhombic pillars or plates are ob- tained. This combination is rather easily soluble in pure water,, but is considerably less soluble in water containing nitric acid; it may be obtained by treating a concentrated solution of urea with an excess of strong nitric acid free from nitrous acid. On heating this combination it volatilizes without leaving a residue. This compound may be employed with advantage in detecting small amounts of urea. A drop of the concentrated solution is placed on a micro- scope-slide and the cover-glass placed upon it ; a drop of nitric acid is then placed on the side of the cover-glass and allowed to flow under. The forma- tion of crystals begins where the solution and the nitric acid meet. Alkali nitrates may crystallize very similarly to urea nitrate when they are con- taminated with other bodies ; therefore, in testing for urea, the crystals must be identified as urea nitrate by heating and by other means. UREA OXALATE, 2.CO(NH2)2.H2C204. This compound is more sparingly soluble in water than the nitric-acid compound. It is- obtained in rhombic or six-sided prisms or plates on adding a saturated oxalic-acid solution to a concentrated solution of urea. Urea also forms combinations with mercuric nitrate in variable proportions. If a very faintly-acid mercuric-nitrate solution is added to a two-per-cent solution of urea, and the mixture carefully neutralized, a combination is obtained of a constant composition THE URINE. 341 which contains for every 10 parts of urea 7.2 parts mercuric oxide. This compound serves as the basis of LIEBIG'S titration method. Urea combines also with salts, forming mostly crystal lizable combi- nations, as, for instance, with sodium chloride, with the chlorides of the heavy metals, etc. An alkaline but not a neutral solution of urea is precipitated with mercuric chloride. The method of preparing urea from urine is chiefly as follows : Concentrate the urine, which has been faintly acidified with sul- phuric acid, at a low temperature, add an excess of nitric acid, at the same time keeping the mixture cool, press the precipitate well, decompose it in water with freshly-precipitated barium carbonate, dry on the water-bath, extract the residue with strong alcohol, decolorize when necessary with animal charcoal, and filter while warm. The urea which crystallizes on cooling is purified by recrystallization from warm alcohol. A further quantity of urea may be obtained from the mother-liquor by concentration. The urea is purified from contaminating mineral bodies by redissolving in alcohol-ether. If it is only necessary to detect the presence of urea in urine, it is sufficient to concentrate a little of the urine on a watch-glass and after cooling treat with an excess of nitric acid. In this way we obtain crystals of urea nitrate. Quantitative Estimation of Urea in urine. The methods suggested for this purpose are those of LIEBIG, by titration, of HEINTZ and EAGSKY, also that of KJELDAHL, by which the total nitrogen is determined, and those of BUNSEN and KNOP-HUFNER, where urea is intended to be determined as such. Among these methods, that of LIEBIG, which is perhaps the one most frequently employed by physicians, will here be carefully explained. In regard to the others, whose chief points only will be spoken of here, the student is referred to other text-books. LIEBIG'S METHOD is based upon the fact that a dilute solution of mercuric nitrate under proper conditions precipitates all the urea forming a compound of constant composition. As indicator, a soda solution or a thin paste of sodium bicarbonate is used. An excess of mercuric nitrate produces herewith a yellow or yellowish- brown combination, while the combination of urea and mercury is white. PFLUGER has given full particulars of this method; there- fore we will describe PFLUGER'S modification of LIEBIG'S method. As phosphoric acid is also precipitated by the mercuric- nitrate solution, this must be removed from the urine by the addi- 342 PHYSIOLOGICAL CHEMISTRY. tion of a baryta solution before titration. PFLUGEE also suggested that the acidity produced by the mercury solution be neutralized with a soda solution. The liquids necessary for the titration are the following: 1. Mercuric Nitrate Solution. This solution is calculated for a 2$ urea solution, and 20 c. c. of the first should correspond to 10 c. c. of the latter. Each c. c. of the mercury solution corresponds to 0.01 grm. urea. As a small excess of HgO is necessary in the urine to make the final reaction (with alkali carbonate or bicarbon- ate) appear, each c. c. of the mercury solution must contain 0.0772 instead of 0.0720 grm. HgO. The mercury solution contains there- fore 77.2 grms. HgO in one litre. The solution may be prepared from pure mercury or mercuric oxide by dissolving in nitric acid. The solution, freed as completely as possible from an excess of acid, is diluted by the careful addition of water, stirring meanwhile, until it has a specific gravity of 1.10 or a little higher at -|- 20° C. The solu- tion is standardized with a 2% solution of pure urea which has been dried over sulphuric acid, and the operation conducted as will be described later. If the solution is too concentrated, it is corrected by the careful addition of the necessary amount of water, avoiding basic salt, and titrated again. The solu- tion is correct if 19.8 c. c. of it is added at ouce to 10 c. c. of the urea solu- tion and the necessary quantity of normal soda solution (11-1 2 c. c. or more) to completely neutralize the liquid, gives the final reaction when 20 c. c. of the mercury solution have been employed. 2. Baryta Solution. This consists of 1 vol. barium-nitrate and 2 vols. barium-hydrate solution, both saturated at the ordinary temperature. 3. Normal Soda Solution. This solution contains 53 grms. pure anhydrous carbonate in 1 litre of water. According to PFLUGEB, a solution having a specific gravity of 1.053 is sufficient. The amount of this soda solution necessary to completely neutralize the acid set free during the titration is determined by titrating with ;i pure 2$ urea solution. To facilitate operations a table can be made showing the quantity of soda solution necessary when from 10 to 35 c. c. of the mercury solution is used. Before the titration the following must be considered. The chlorides of the urine interfere with the titration in that a part of the mercuric nitrate is transformed into mercuric chloride, which does not precipitate the urea. The chlorides of the urine are there- fore removed by a silver-nitrate solution, which also removes any bromine or iodine combinations which may exist in the urine. If the urine contains albumin in noticeable amounts, it must be removed by coagulation and the addition of acetic acid, but care must be taken that the concentration and the volume of the urine is not Changed during these operations. If the urine contains ammonium carbonate in notable quantities, caused by alkaline THE URINE. 343 fermentation, this titration method cannot be applied. The same is true of urine containing leucin, tyrosin or medicinal preparations, precipitated by mercuric nitrate. In cases where the urine is free from albumin or sugar and not specially poor in chlorides, the quantity of urea, and also the approximate quantity of mercuric nitrate necessary for the titration, may be learned from the specific gravity. A specific gravity of 1.010 corresponds to about 10 p. m., the specific gravity 1.015 fenerally somewhat less than 15 p. m., and the specific gravity .015-1.020 about 15-20 p. m. urea. With a specific gravity higher than 1.020 the urine generally contains more than 20 p. m. of urea, and above this point the amount of urea increases much more rapidly than the specific gravity, so that at a specific gravity of 1.030 it contains over 40 p. m. urea. In fevers, urine with a specific gravity above 1.020 sometimes contains 30-40 p. m. urea, or even more. PREPARATION FOR THE TITRATION. If a large amount of urea is suspected from a high specific gravity, the urine must first be diluted with a carefully-measured quantity of water, so that the amount of urea is reduced below 30 p. m. In a special portion of the same urine the amount of chlorides is determined by one of the methods which will be given later, and the number of c. c. of silver- nitrate solution necessary is noted. Then a larger quantity of urine, say 100 c. c., is mixed with one half or, if this is not sufficient to precipitate all the sulphuric and phosphoric acids, with an equal vol- ume of the baryta solution, it is then allowed to stand a little while, and the precipitate is filtered through a dried filter. From the fil- trate containing the urine diluted with water a proper quantity, corresponding to about 60 c. c. of the original urine, is measured, and exactly neutralized with nitric acid added from a burette, so that the exact quantity employed is known. The neutralized mix- ture of urine and baryta is treated with the proper quantity of silver-nitrate solution necessary to completely precipitate the chlo- rides, which was ascertained by a previous determination. The mix- ture containing a known volume of urine is now filtered through a dried filter into a flask, and from the filtrate an amount is measured corresponding to 10 c. c. of the original urine. EXECUTION OF THE TITRATION. Nearly the total quantity of mercuric-nitrate solution to be used, and which is known from the specific gravity of the urine, is added at once, and immediately afterwards the quantity of soda solution necessary, as indicated by the table. If the mixture becomes yellowish in color, then too much mercury solution has been added and another determination must be made. If the test remains white, and if a drop taken out and placed on a glass plate with a dark background and stirred with a drop of a thin paste of sodium bicarbonate does not give a yellow 344 PHYSIOLOGICAL CHEMISTRY. color, the addition of mercury solution is continued by adding ^ and then -f^ c. c., and testing after each addition in the following way : A drop of the mixture is placed on a glass plate with a dark background beside a small drop of the bicarbonate paste. If the color after stirring the two drops together is still white after a few seconds, then more mercury solution must be added; if, on the con* trary, it is yellowish, then — if not too much mercury solution has been added by inattention — the result to TV c. c. has been found. By this approximate determination, which is sufficient in many cases, we have learned the minimum amount of mercury solution necessary to add to the quantity of urine in question, and we now proceed to the final determination. A second quantity of the filtrate, corresponding to 10 c. c. of the original urine, is filtered, and the same quantity of mercury solution added at one time as was found necessary to produce the final reaction, and immediately after the corresponding amount of soda solution, which must not indicate the end of the reaction. Then add the mercury solution in -^ c. c. without neutralizing witli soda, until a drop taken out and mixed with the soda solution gives a yellow coloration. If this final reaction is obtained after the addition of 0.1-0.2 c. c., then the titration may be considered as finished. If, on the contrary, a larger quantity is necessary, the addition of the mercury solution must be continued until a final reaction is ob- tained with simple carbonate, and the titration repeated again, add- ing the quantity of mercury solution used in the previous test at one time, and also adding the corresponding amount of soda solu- tion. If we obtain the end reaction by the addition of ^ c. c., we may consider the titration as finished. If in each titration a quantity of filtrate containing urine and baryta corresponding to 10 c. c. of the primitive urine is used, then the calculations are very simple, since 1 c. c. of mercuric-nitrate solution corresponds to 0.01 grm. of urea. As the mercury solu- tion is made for a 2$ urea solution, the filtrate of urine and baryta being generally deficient in urea (if the quantity of urea is above 2$, it is easy to avoid any mistake by diluting the urine at the beginning of the operation), a mistake occurs here which can be cor- rected in the following way, according to PFLUGER : To the measured volume of the filtrate from the urine (the filtrate with baryta after neutralization with nitric acid, precipitation with silver nitrate and filtration) the quantity of normal soda solution employed is added, and from this sum the volume of mercury solution used is sub- tracted. The remainder is then multiplied by 0.08, and the prod- uct subtracted from the number of c. c. of mercury solution used. For example, if the filtrate (urine and baryta + nitric acid -j- silver nitrate) measured 25.8 c. c., and the number of c. c. of soda solu- tion used in the titration, 13.8 c. c., and the mercury solution, 20.5 THE URINE. 345 c. c., we have then 20.5 - {(39.6 - 20.5)x0.08( = 20.5 - 1.53 = 18.97, and the corrected quantity of mercury solution is therefore '18.97 c. c. If the measured c. c. of the filtrate (in this case 25.8 c. c.) corresponds to 10 c. c. of the original urine, then the amount of urea is 18.97 X 0.01 = 0.1897 = 18.97 p. m. urea. Besides the urea other nitrogenized constituents of the urine are precipitated by the mercury solution. By the titration we really do not obtain the quantity of urea, but, as PFLUGER has shown, the total quantity of nitrogen in the urine expressed ad urea. As the urea contains 46.67 p. c. N, the total quantity of nitrogen in the urine may be calculated from the quantity of urea found. The results obtained by LIEBIG-PFLUGER'S titration method for the total nitrogen, PFLUGER has shown, correspond well with the results obtained by KJELDAHI/S method, which was first (1861) used by ALMEN for1 urea determinations, and modified by PFLUGER and BORLAND. This method consists in heating the urine a cer- tain time with an excess of concentrated or fuming sulphuric acid (5 c. c. urine and 40 c. c. sulphuric acid) until all the nitrogen has been converted into ammonia, and after the addition of an excess of caustic soda the ammonia is distilled into ^ sulphuric acid and the amount of ammonia distilled over is determined by titration. BUNSEN'S UREA DETERMINATION. The principle of this method consists in heating the urine or urea solution in a sealed glass tube to a high temperature with an alkaline barium-chloride solution. The urea splits into carbon dioxide and ammonia, which may be determined separately. This method has been very care- fully tested by PFLUGER and his pupils BORLAND and BLEIBTREU, and essentially improved. They found that very accurate results can be obtained by this method if the other nitrogenized constit- uents of the urine are first precipitated by a mixture of hydro- chloric acid and phospho-tungstic acid, and then the filtrate made faintly alkaline with milk of lime, and lastly heated with alkaline barium-chloride solution in a sealed tube. The carbon dioxide and the ammonia can be determined (by distilling with magnesia and receiving the distillate in — acid and titrating). In the last 346 PHYSIOLOGICAL CHEMISTRY. case a correction must be made (according to SCHLOSING'S method) for the ammonia pre-existing in the urine. PFLUGER and BLEIBTREU have essentially changed this method in the fol- lowing way : They precipitate the other nitrogenized urinary con- stituents with hydrochloric acid and phospho-tungstic acid, make the filtrate faintly alkaline with milk of lime, determine the pre-existing ammonia in a part of this filtrate according to SCHLOSING'S method (observing certain precautions), and then placing the other part of the filtrate (about 15 c. c.) in a large flask which contains 10 grms. crystallized phosphoric acid, heat fo 230°-260° C. for about three hours. All the urea is decomposed, and the ammonia split off combines with the phosphoric acid. After cooling, an excess of caustic soda is added and the ammonia distilled into a titrated acid, which must then be retitrated. After subtracting the quantity of pre-existing ammonia very accurate results are obtained for the ammonia originating from the urea (and perhaps from an unknown ureid present in the urine). KNOP-HUF^ER'S METHOD is based on the fact that urea by the action of sodium hypobromite splits into water, carbon dioxide (which dissolves in the alkali), and nitrogen, whose volume is measured (see page 340). This method is less accurate than the preceding ones, and therefore in scientific work it is discarded. It is of value to the physician and for practical purposes because of the ease and rapidity with which it may be performed, even though it may not give very accurate results. For practical purposes a series of different apparatus have been constructed to facilitate the use of this method. Among these the ureometer of ESBACH deserves to be especially mentioned.1 In regard to the reagents 1 Dr. CHAS. A. DOREMUS has constructed a ureometer of the very simplest kind. It consists of two parts. First, a vertical glass tube closed at the top and bent sharply below, where it expands into a bulb having an orifice at its upper part. Secondly, a pipette with an elastic rubber nipple. Both parts are graduated ; the vertical tube so as to show the quantity of urea (as indi- cated by the volume of nitrogen) in each cubic centimetre of urine tested, while the pipette is graduated so as to show one cubic centimetre. The instrument is used thus: First the vertical tube is filled with the alkaline sodium-hypobromite solution in the following manner : holding it vertically, the operator pours the solution into the bulb, and when it is rather more than half full he inclines the apparatus horizontally until the entire tube is filled and a little left in the bulb— say one third or thereabouts. Then he restores it to the vertical position. He now draws into the pipette, by means of the THE URINE. 347 necessary for the determination of urea, and also for instructions in •the use of this instrument, we must refer the reader to the direc- tions accompanying the apparatus. For pure urea solutions Es- BACH'S apparatus gives quite exact results. The determination of urea in urine by this method always gives results somewhat too low, and as a rule a result is obtained which on an average is about 0.1$ lower than that obtained with LIEBIG'S titration method. Creatinin, C4H,N30, or NH : tX^oiijIjH,' *s generally con- sidered as the anhydride of creatin (see page 257) found in the muscles. It occurs in human urine and in that of certain mam- malia. It has also been found in ox-blood (VoiT), milk, though in very small amounts (WEYL), and in the flesh of certain fishes. The quantity of creatinin in human urine is for a grown man, voiding a normal quantity of urine in the 24 hours, 0.6-1.3 grms. (NEUBAUER), or on an average 1 grm. The quantity is dependent on the food, and decreases in starvation. Sucklings do not gener- ally eliminate any creatinin, and it only appears in the urine when the milk is replaced by other food. The quantity of creatinin in urine varies as a rule with the quantity of urea, although it- is in- creased, more by flesh (because the flesh contains creatin) than by albumin. GROCCO claims that the elimination of creatinin is in- creased by muscular activity, which is contrary to the statements of HOFMANN and others. The behavior of% creatinin in disease is little known. By increased exchange of material the amount is increased, while by decreased exchange of material, as in anaemia and cachexia, it is diminished. Creatinin crystallizes in colorless, shining monoclinic prisms which differ from creatin crystals in not becoming white with loss of water when heated to 100° C. It dissolves in 11.5 parts cold water, but more easily in warm water. It requires nearly 100 parts rubber nipple, a cubic centimetre of the urine to be tested, which should be taken from the total excretion of the twenty four hours, the exact quantity of which should be noted. The end of the pipette is passed into the bulb until the point is exactly under the vertical tube, and slowly compress the nipple. The urine being lighter than the hypobromile solution rises through it, and on its way the contained urea is decomposed and its nitrogen set free. The quantity of urea contained in the one cubic centimetre of urine used is rend off on the instrument. — TRANSLATOR. 348 PHYSIOLOGICAL CHEMISTRY. cold absolute alcohol for solution, but it is more soluble in warm alcohol. It is nearly insoluble in ether. In alkaline solution crea- tinin is converted into creatin very easily on warming. Creatinin gives an easily-soluble crystalline combination with hydrochloric acid. A solution of creatinin acidified with mineral acids gives crystalline precipitates with phospho-tungstic or phos- pho-molybdic acids, even in very dilute solutions (1 : 10,000) (KER- KEE). It is precipitated, like urea, with mercuric-nitrate solution. Among the compounds of creatinin, that with zinc chloride, crea- tinin zinc chloride (C4H7N30)2ZnCl2 , is of special interest. This combination is obtained when a sufficiently concentrated solution of creatinin in alcohol is treated with a concentrated, faintly-acid solution of zinc chloride. Free mineral acids dissolve the combina- tion, but this, however, is prevented, when they are present, by an addition of sodium acetate. In the impure state, as ordinarily ob- tained from urine, creatinin zinc chloride forms a sandy, yellowish powder which under the microscope appears as fine needles 'form- ing concentric groups, mostly complete rosettes or yellow balls or tufts, or grouped as brushes. On slowly crystallizing, or when very pure, more sharply-defined prismatic crystals are obtained. This combination is sparingly soluble in water. Creatinin acts as a reducing agent. Mercuric oxide is reduced to metallic mercury, and oxalic acid and methylguanidin (methyl- uramin) are formed. Creatinin also reduces copper hydroxide in alkaline solution, forming a colorless soluble combination, and only after continuous boiling with an excess of copper salts is free sub- oxide of copper formed. Creatinin interferes with TROMMER'S test for sugar, partly because it has a reducing action and partly by retaining the copper suboxide in solution. The combination with copper suboxide is not soluble in a saturated-soda solution and if a little creatinin is dissolved in a cold, saturated-soda solution and then a few drops of FEHLIISTG'S reagent added, a white flocculent combination separates after heating to 50°-60° C. and then cooling (v. MASCHKE'S reaction). An alkaline bismuth solution (see Sugar •Tests) is not reduced by creatinin. If we add a few drops of a freshly-prepared very dilute sodium nitroprusside (sp. gr. 1.003) to a dilute creatinin solution (or to the urine) and then a few drops of caustic soda, a ruby-red liquid THE URINE. 349 is obtained which quickly turns yellow again (WEYI/S reaction). If we use ammonia instead of caustic soda in this reaction, the red color is not obtained (differing from aceton and ethyl-diacetic acid, LE NOBEL). If the above solution, which has become yellow, is treated with an excess of acetic acid and heated, the solution be- comes first green and then blue (SALKOWSKI) ; finally a precipitate of Prussian blue is obtained. If a solution of creatinin in water (or urine) is treated with a watery solution of picric acid and a few drops of a dilute soda solution, a red coloration lasting several hours occurs immediately at the ordinary temperature, and which turns yellow on the addition of acid (JAFFE'S reaction). Acetone gives a more reddish-yellow color. Grape-sugar gives with this reagent a red coloration only after heating. In preparing creatinin from urine the creatinin zinc chloride is first prepared according to NEUBAUER'S method, and this method is also employed for the quantitative estimation of creatinin. In making a quantitative estimation 200-300 c. c. of urine freed from albumin (by boiling with acid) and from sugar (by fermentation with yeast) are measured, alkalized with milk of lime, and treated with CaCl2 solution until all the phosphoric acid is precipitated ; it is filtered and washed with water, the filtrate and the wash-water united, and evaporated to a syrup after acidifying with acetic acid. This syrup is mixed while hot with 50 c. c. of 95$-97$ alcohol. This mixture is transferred to a beaker, and the residue in the evaporating-dish is completely and carefully removed and added. The liquid is allowed to stand covered for at least eight hours in the cold. Then it is filtered through a small filter, the precipitate washed with alcohol, the filtrate evaporated if necessary until the volume is 50-60 c. c., then allowed to cool and £ c. c..of an acid- free zinc-chloride solution of an sp. gr, of 1.20 is added ; it is stirred, and the covered beaker is left standing in a cool place for two or three days. The precipitate is collected on a small dried and weighed filter, using the filtrate to wash the crystals from the beaker. After allowing the crystals to completely drain off, they are washed with a little alcohol until the filtrate gives no reaction for chlorine, and dried at 100° C. 100 parts creatinin zinc chloride contain 62.42 parts creatinin. As the precipitate is never quite pure, the quantity of zinc must be carefully determined, in exact- experiments, by evaporating with nitric acid, heating, washing the oxide of zinc with water (to remove any NaCl), drying, heating, and weighing. 22.4 parts zinc oxide correspond to 100 parts crea- tinin zinc chloride. 350 PHYSIOLOGICAL CHEMISTRY. The preparation of creatinin zinc chloride on a large scale from urine is done in the same way. The creatinin is obtained from the creatinin zinc chloride by boiling with lead hydroxide, filtering, decolorizing the filtrate with animal charcoal, evaporating, treating the residue with strong alcohol (which leaves the creatin undis- solved), evaporating to crystallization, redissolving in water, and recrystallizing. Xanthocreatinin. This body, which has been spoken of in a previous chap- ter on the muscles, has been found in dog's urine after the injection of crea- tinin into the abdominal cavity (MoNAKi), and in human urine after several hours of continuous marching. The correctness of these observations is disputed by STADTHAGEN. Uric Acid, Ur, CsH^Os. The structural formula of this acid, according to MEDICUS, is CO/ C.NH/CO, and this acid \NH.CO may therefore be considered, from its constitution as a derivate of acrylic acid, as acrylic acid diureid. Uric acid has been synthetically prepared by HORBACZEWSKI in several ways. On fusing urea and glycocoll, uric acid is formed according to the formula 3CON2H4 + C2H6N02 = C6H,N~A + 2H20 + 3NH3, and in this reaction hydantoin and biuret are formed as intermediate products. On melting methylhydantoin with urea or methylhydantoin with biuret or with allophanic-acid amyl-ester HORBACZEWSKI obtained methyl-uric acid. He also obtained uric acid on heating trichlor-lactic acid, or still better trichlor-lactic acid- amid, with an excess of urea. If we eliminate from the reaction the numerous by-products (cyanuric acid, carbon dioxide, etc.), then this process may be expressed by the formula CsClgH^OaN -f- 2CON2H, = C.H4N40, + H20 + NH4C1 -f 2-H01. On strongly heating uric acid it decomposes with the formation of UREA, HYDROCYANIC ACID, CYANURIC ACID, and AMMONIA. On heating with concentrated hydrochloric acid in sealed tubes to 170° C. it splits into GLYCOCOLL, CARBON DIOXIDE, and AMMONIA. By the action of oxidizing agents a splitting and oxidation takes place, and either monoureid or diureid is produced. By oxidation with lead peroxide, CARBON DIOXIDE, OXALIC ACID, UREA, and ALLANTOIN, which last is glyoxyl diureid, are produced (see below). By oxidation with nitric acid in the cold UREA and a monoureid, the THE URINE. 351 mesoxalyl urea or ALLOXAN, are obtained, C5H4N403 + 0 -f H20 = , C4H2N204 -+- (NH2)200. On warming with nitric acid, uric acid yields alloxan, carbon dioxide, and oxalyl urea or PARABANIC ACID, C3H2N203. By the addition of water the parabanic acid passes into OXALURIC ACID, CaHJ^O^ , traces of which are found in the urine and which easily split into oxalic acid and urea. Uric acid occurs most abundantly in the urine of birds and of scaly amphibians, in which animals the greater part of the nitrogen of the urine appears in this form. Uric acid occurs frequently in the urine of carnivorous mammalia, but is sometimes absent; in urine of herbivora it is habitually present, though only as traces; in human urine it occurs in greater but still small and variable amounts. Traces of uric acid are also found in several organs and tissues, as in the spleen, lungs, heart, pancreas, liver (especially in birds), and in the brain. It habitually occurs in the blood of birds (MEISSNER). Traces have been found in human blood under normal conditions (ABELES), but especially in gout (GARROD). Uric acid also occurs in large quantities in "chalk-stones," certain urinary calculi, and in guano. It has also been detected in the urine of insects and certain snails. The amount of uric acid eliminated with the human urine is subject to considerable variation, but amounts on an average to 0.7 grm. during 24 hours on a mixed diet. On a vegetable diet the amount is smaller, and on an abundant meat diet it may rise to 2 grms. and over. The relationship of the uric acid to the urea on a mixed diet is on an average 1 : 50-1 : 70. In new-born infants and in the first days of life the elimination of uric acid is increased (MARES), and the relation between the uric acid and urea is about 1 : 13-14. After taking glycerin the amount of uric acid is in- creased (HORBACZEWSKI and KANERA), while it is not increased by sodium acrylate (HORBACZEWSKI). Uric acid when introduced into the organism of a dog is in great part converted into urea, and as urea is also formed by the action of oxidizing agents on uric acid outside of the body, uric acid has been often considered as a step towards the formation of urea in the organism. Such a view is not, however, well founded, and the statement that an incomplete supply of oxygen and diminished oxidation cause an increased formation of uric acid has not been 352 PHYSIOLOGICAL CHEMISTRY. proved. With regard to the pathological relations we really only know two conditions in which the elimination of uric acid is increased, namely, in fever and leucaemia. In fevers the elimina- tion of uric acid is increased because of an increased waste of the organism. In leucaemia the elimination is increased absolutely as well as relatively to the urea (RANKE, SALKOWSKI, FLEISCHER and PENZOLDT, STADTHAGEN, and others), and the relationship of the elimination of uric acid to the urea may be 1 : 16-1 : 12. The elimination of uric acid may be diminished in gout shortly before and during the attack, because the uric acid is retained in the body. A decrease in the elimination of uric acid is observed also in a diminished exchange of material, also after the use of quinine, caffeine, and certain other medicines. Formation of Uric Acid in the organism. The formation of uric acid in birds is increased by the administration of ammonia-salts (v. SCHRODER). Urea acts in the same way (MEYER and JAFFE), while in the organism of mammalia uric acid is more or less com- pletely converted into urea. MLFKOWSKI observed in geese with their livers extirpated a very significant decrease in the elimination of uric acid, while the elimination of ammonia is increased to a corresponding degree. This indicates a participation of ammonia in the formation of uric acid in the organism of birds ; and as MIKKOWSKI has also found after the extirpation of the liver that considerable amounts of lactic acid occur in the urine, it is prob- able that the uric acid in birds is produced in the liver, perhaps from lactic acid and ammonia by synthesis. Amido-acids — leucin, glycocoll, and aspartic acid — increase the elimination of uric acid in birds (v. KNTERIEM), but whether the amido-acids are first decom- posed with the splitting off of ammonia is still unknown, v. MACH has shown that a small part of the uric acid in birds originates from hypoxanthin, and a similar origin for the uric acid of mammalia is also very probable (MINKOWSKI). After the extirpation of the kidneys of snakes and birds V. SCHRODER has observed an accumulation of uric acid in the blood and tissues. . This shows that the kidneys of these animals are not the only source of uric acid, and any direct proof of the formation of this acid in the kidneys has not to the present time been demon- strated. A direct relationship between the spleen and the forma- THE URINE. 353 tion of uric acid, also in man, has been sought by many investigators (RAKKE, KUHNE), and in support of this view they claim that the elimination of uric acid is increased in diseases in which the spleen is enlarged, also that the elimination of uric acid by the urine is decreased when the volume of the spleen is diminished by the administration of large quantities of quinine. That uric acid is formed in the spleen receives additional proof from the investiga- tions of HORBACZEWSKI. He found a considerable new formation of uric acid vjhen he allowed the pulp of the spleen and blood of calves to act upon each other at the ordinary temperature, at the same time passing a stream of air through the mass. He also obtained extracts from the pulp of the spleen with boiling water, which yielded uric acid after the action of the blood. This produc- tion must depend chiefly upon decomposition products of nuclein (xanthin bodies). HORBACZEWSKI claims that probably the lymphatic elements are here concerned, a statement which coincides with the increased elimination of uric acid in lineal leucaemia, also with the parallelism existing between the digestion-leucocytes and the increased elimination of uric acid occurring after taking food. We have no positive basis for the statement that uric acid is formed in the liver of man and mammalia, but the formation of uric acid in the liver of birds is shown to be highly probable by the researches of MINKOWSKI. Properties and Reactions of Uric Acid. Pure uric acid is a white, odorless, and tasteless powder consisting of very small rhombical prisms or plates. Impure uric acid is easily obtained as somewhat larger, colored crystals. In quick crystallization, small, apparently colorless, thin, four- sided rhombic prisms, which can only be seen by the aid of the microscope, are formed, and these sometimes appear as spools because of the rounding of their obtuse angles. The plates are sometimes six-sided, irregularly developed; in other cases they are rectangular with partly straight and partly jagged sides; and in other cases they show still more irregular forms, the so-called dumb- bells, etc. In slow crystallization, as when the urine deposits a sediment or when treated with acid, large, always colored crystals separate. Examined with the microscope these crystals appear always yellow or yellowish .brown in color. The most ordinary 354 PHYSIOLOGICAL CHEMISTRY. form is the whetstone shape formed by the rounding off of the obtuse angles of the rhombic plate. The whetstones are numerous, connected together, two or more crossing each other. Besides these forms, rosettes of prismatic crystals, irregular crosses, brown-colored rough masses of crystals which assume the shape of needles and prisms occur, also other different forms. Uric acid is insoluble in alcohol and ether; it is rather easily dissolved in boiling glycerin, very difficultly soluble in cold water (14,000-15,000 parts); and difficultly soluble in boiling water (in 1800-1900 parts), it is soluble in a warm solution of sodium diphosphate, and in the presence of an excess of uric acid mono- phosphate and acid urate are produced. Sodium phosphate is considered as a solvent for the uric acid in the urine. Uric acid is dissolved without decomposing in concentrated sulphuric acid. It is completely precipitated from the urine by picric acid (JAFFE). Uric acid forms with bases two series of salts, neutral and acid. Of the alkali urates the neutral potassium and lithium salts dis- solve the most easily, and the ammonium salt dissolves with the most difficulty. The acid-alkali urates are very insoluble and separate as a sediment (sedimentum lateritium) from concentrated urine on cooling. The salts with alkaline earths are very insoluble. If a little uric acid in substance is treated with a few drops of nitric acid, the uric acid dissolves on warming with a strong development of gas, and after thoroughly drying on the water-bath a beautiful red residue is obtained, which turns a purple-red (pur- purate of ammonium) on the addition of a little ammonia. If, instead of the ammonia, we add a little caustic soda (after cooling), the color becomes more blue or bluish violet. This color disappears quickly on warming, differing from certain xanthin bodies. This reaction is called the murexide test. Uric acid does not reduce an alkaline solution of bismuth, but does, on the contrary, an alkaline copper-hydroxide solution. In the presence of only a little copper salt we obtain a white precip- itate consisting of copper urate. In the presence of more copper salt red suboxide separates. If a drop of uric acid dissolved in sodium carbonate is placed on a piece of filter-paper which has been previously treated with silver-nitrate solution, a reduction of silver oxide occurs producing THE URINE. 355 a brownish-black or, in the presence of only 0.002 milligram uric acid, a yellow spot (SCHIFF'S test). Preparation of Uric Acid from Urine. Filtered normal urine is treated with 20-30 c. c. of 25$ hydrochloric acid for each litre of urine. After forty-eight hours collect the crystals and purify them by redissolving in dilute alkali, decolorizing with animal char- coal and reprecipitating with hydrochloric acid. Large quantities of uric acid are easily obtained from the excrements of serpents by boiling them with dilute caustic potash until no more ammonia is developed. A current of carbon dioxide is passed through the filtrate until it barely has an alkaline reaction; dissolve the separated and washed acid potassium urate in caustic potash, and precipitate the uric acid by addition of an excess of hydrochloric acid to the filtrate. Quantitative Estimation of Uric Acid in the urine. The older method of HEINTZ, somewhat modified by SCHWANERT, is in its- main points as follows : The filtered urine free from albumin and any sediment of urates (dissolved by warming) is concentrated when too dilute to a sp. gr. of 1.020, 200 c. c. measured off and this treated with 10-20 c. c. hydrochloric acid of a sp. gr. of 1.12. After allowing this mixture to stand forty-eight hours in a cool place the precipitated uric acid is collected on a small weighed filter (5-6 cm. diameter), and the crystals which have adhered to the sides of the glass are removed by means of a glass rod tipped with a piece of rubber tubing, using the filtrate to wash with. After all the liquid has passed through, fill the filter with water and allow it to run through completely before adding more water; continue until the wash-water does not give a chlorine reaction, then dry and weigh. A part of the uric acid always remains dis- solved in the filtrate. The filtrate, including the wash-water, must therefore be measured, and for each 10 c. c. of filtrate (and wash- water) 0.00048 grm. uric acid must be added. With this correc- tion this determination gives the same results as the following more complicated method. In SALKOWSKI and LTJDWIG'S method the uric acid is precip- itated from the urine by silver-nitrate solution, treated with magnesium mixture, and the uric acid removed from the silver precipitate and weighed. Uric-acid determinations according to this method are often performed according to the treatment sug- gested by E. LUDWIG, which requires the following solutions : 1. An AMMONIACAL SILVER-NITRATE solution which contains in one litre 26 grms. silver nitrate and a quantity of ammonia sufficient to completely reklissolve the precipitate produced by the first addition of ammonia. 2. MAGNESIUM MIXTURE. Dissolve 100 grms. crystallized magnesium chlo- ride in water and add enough ammonia so^that the liquid smells strongly of it, 356 PHYSIOLOGICAL CHEMISTRY. and then add sufficient ammonium chloride to dissolve the precipitate and lastly dilute to 1 litre. 3. SODIUM- SULPHIDE SOLUTION. Dissolve 10 grms. caustic soda which is free from nitric and nitrous acids in 1 litre of water. One half of this solution is completely saturated with hydrogen sulphide and then mixed with the other half. The solutions are such that 10 c. c. of each is sufficient for 100 c. c. of the urine. 100-200 c. c., according to concentration, of the filtered urine freed from albumin (by boiling after the addition of a few drops of acetic acid) are poured into a beaker. In another vessel mix 10-20 c. c. of the silver solution with 10-20 c. c. of the magnesium mix- ture, and add ammonia, and when necessary also some ammonium chloride, until the mixture is clear. This solution is added to the urine while stirring, and the mixture allowed to stand quietly for J- hour. The precipitate is collected on a filter, washed with am- moniacal water, and then returned to the same beaker by the aid of a glass rod and a spirit-bottle without destroying the filter. Now heat to boiling-point 10-20 c. c. of the alkaline sulphide solution, which has previously been diluted with an equal amount of water, and allow this solution to flow through the above filter into the beaker containing the silver precipitate, wash with boiling water, and warm the contents of the beaker on the water-bath for a time, stirring constantly. After cooling filter into a porcelain dish, wash with boiling water, acidify the filtrate with hydrochloric acid, evap- orate to about 15 c. c., add a few drops more of hydrochloric acid, and allow it to stand for 24 hours. The uric acid which has crys- tallized is collected on a small weighed filter, washed with water, alcohol, ether, and carbon disulphide, dried at 100°-110° C., and weighed. For each 10 c. c. of the watery filtrate we must add 0.00048 grm. uric acid to the amount found directly. Instead of the weighed filter-paper a glass tube filled with glass wool may be substituted. This tube was constructed by LUDWIG, and is' de- scribed in other hand books. HAYCRAFT'S METHOD: 25 c. c. of the urine are first treated with 1 grm. bicarbonate, then made strongly alkaline by ammonia, and lastly precipitated by an ammoniacal silver solution. The carefully washed precipitate is dissolved in 20-30$ nitric acid and n this solution titrated with a j^ sulphocyanide solution according to VOLHARD'S method. Each c. c. of this solution corresponds to 0.00168 grm. uric acid. This method has been modified by CZA- PEK. After the addition of a known amount of silver solution of known strength, the amount of silver salts remaining in the urine after all the uric acid has been precipitated is titrated with alkali sulphide. The advantage of HAYCRAFT'S method is the ease and rapidity with which it can be performed, and it is therefore recom- THE URINE. 357 mended for clinical purposes. For exact determinations it is not quite reliable, because the amount of silver in the precipitate of 'silver urate is not constant (SALKOWSKI). Since the value of this method has been the subject of much adverse criticism, we will not give further particulars. OXALURIC ACID, C3H4N2O4=(CONaH3).CO.COOH. This acid, whose relation to uric acid and urea has been spoken of above, occurs only as traces in the urine as ammonium salts. This salt is not directly precipitated by CaCls and NHS , but after boiling, when it is decomposed into urea and oxalate. In preparing oxaluric acid from urine the latter is filtered through animal char- coal. The oxalurate retained by the charcoal may be obtained by boiling with alcohol. POOTT Oxalic Acid, C8H804, or QQQTT> occurs under physiological conditions in very small amounts in the urine, about 0.02 grm. in 24 hours (FuRB RINGER). According to the generally-received opinion it is found in the urine as calcium oxalate, which is kept in solution by the acid phosphates present. Calcium oxalate is a frequent constituent of the urinary sediments, and occurs also in certain urinary calculi. The origin of the oxalic acid in the urine is not well known. Oxalic acid, when administered, is eliminated by the urine un- changed, and as many vegetables and fruits, such as cabbage, spin- ach, asparagus, sorrel, apples, grapes, etc., contain oxalic acid, it is possible that a part of the oxalic acid of the urine originates directly from the food. Another part is certainly formed in the body from the proteids and fat or by the incomplete combustion of the car- bohydrates. The formation of oxalic acid from proteids (or fat) is inferred from the fact that oxalic acid is eliminated by the urine after food consisting entirely of flesh and fat, as also in starvation. It has also been considered, but without sufficient reason, that the oxalic acid of the urine is an oxidation product of uric acid. An increased elimination of oxalic acid may occur in diabetes. The question whether it may occur as an independent disease (oxaluria, oxalic-acid diathesis) has not been positively decided. The properties and reactions of oxalic acid and calcium oxalate are well known. The calcium oxalate as a constituent of urinary sediments will be described later. Defection and quantitative estimation of oxalic acid in urine. The presence of oxalic acid in solution in urine is determined 358 PHYSIOLOGICAL CHEMISTBY. according to the method suggested by NEUBAUER, who treats 500-600 c. c. of the urine with CaCl2 solution, makes alkaline with ammonia and then faintly acid with acetic acid. After 24 hours the precipitate is collected on a small filter, washed with water, treated with hydrochloric acid (which leaves the uric acid undis- solved on the filter), and washed again with water. The filtrate, including the wash- water, is treated with an excess of ammonia and allowed to stand 24 hours. Calcium oxalate separates as quadratic octahedra. The quantitative estimation is performed after the same principle. The oxalate is converted into quick-lime by heat, and weighed as such. Allantoin or GLYOXYLDIUEEID, C4H6N403 or ,^/NH.CH.NH.CO.NH, C0< ^TT pr\ > occurs in the urine of children within the first eight days after birth, and in very small amounts also in the urine of grown persons (GussEROW, ZIEGLEB and HERMANN). It is found in rather abundant quantities in the urine of pregnant women (GUSSEROW). Allantoin has also been found in the urine of sucking calves (WOHLER), and sometimes in the urine of other animals (MEISSKER). It is also found in the amniotic fluid and allantoic fluid of the cow (hence the name). Allantoin is formed, as above stated, by the oxidation of uric acid. The increased elim- ination of allantoin which SALKOWSKI observed in dogs after the administration of uric acid shows that the formation of allantoin from uric acid in the organism is not improbable. Allantoin is a colorless substance often crystallizing in prisms, difficultly soluble in cold water, easily soluble in boiling water and also in warm alcohol, but not soluble in cold alcohol or ether. It combines with acids, forming salts. A watery allantoin solution gives no precipitate with silver nitrate alone, but by the careful addition of ammonia a white flocculent precipitate is formed, C4H5AgN403, which is soluble in an excess of ammonia and which consists after a certain time of very small, transparent microscopic globules. The dried precipitate contains 40.75$ silver. A watery allantoin solution is precipitated by mercuric nitrate. Allantoin is most easily prepared by the oxidation of uric acid with lead peroxide. In preparing allantoin from calves' urine, con- centrate the urine on the water-bath to a syrup and allow it to stand in the cold for several days. The crystals which are sepa- THE URINE. 359 rated from the precipitate by washing are dissolved in boiling water with the addition of some animal charcoal, and filtered while hot ; then acidify the filtrate faintly with hydrochloric acid (so as to keep the phosphates in solution) and allow it to crystallize. Al- lantoin is detected in human urine by the method first suggested by MEISSNER. It consists chiefly of the following points: Pre- cipitate the urine with baryta- water, filter, remove the baryta with sulphuric acid, filter, precipitate the allantoin with Hg012 in alka- line solution, decompose the precipitate with sulphuretted hydro- gen, concentrate strongly, purify the crystals which separate by recrystallization, and lastly prepare the silver combination. Xanthin Bodies. The xanthin bodies which habitually occur in human urine are xantliin, hypoxanthin (SALOMON), guanin (POUCHET), carnin (POUCHET), and the newly-discovered bodies paraxanthin (THUDICHUM, SALOMON) and heteroxanthin (SALO- MON). The quantity of these bodies in the urine is very small. The quantity of xanthin bodies in the urine is increased especially in leucaemia, in which disease adenin is also found in the urine (STADTHAGEN). Xanthin also occurs as a constituent of a variety of rare calculi (MARCET). It is also sometimes found as a constitu- ent of urinary sediments (BENCE JONES). Paraxanthin, GMIp^Oa (dimethylxanthin), and heteroxanthin, C8H«N4O2 (methylxanthin), do not give the xanthin reaction with nitric acid and alkali, but give WEIDEL'S reaction (see page 50). They differ from other xanthin bodies by forming crystalline combinations with alkalies, which are diffi- cultly soluble. Amorphous heteroxanthin separates on neutralizing the so- dium combination, but paraxanthin, on the contrary, separates in a crystalline condition. Paraxanthin gives an easily-soluble combination with hydro- chloric acid, while heteroxanthin forms an insoluble, beautiful crystalline combination. In preparing xanthin bodies from the urine, it is supersaturated with ammonia and precipitated by a silver-nitrate solution. The precipitate is then decomposed with sulphuretted hydrogen. The boiling-hot filtrate is evaporated to dryness and the dried residue treated with 3# sulphuric acid. The xanthin bodies are dissolved, while the uric acid remains uudissolved. This filtrate is saturated with ammonia and precipitated by silver-nitrate solu- tion. The different xanthin bodies may be separated from each other by treating the silver precipitate with boiling-hot nitric acid of a sp. gr. of 1.1 (see page 51). Hippuric acid, or BENZOYL-AMIDO ACETIC ACID, C9H9N03 or C6H5.CO.NH.CH2.COOH. This acid decomposes into benzoic acid and glycocoll on boiling the urine with mineral acids or alkalies, this also takes place in putrefaction. The reverse of this occurs if these two components are heated in a sealed tube, according 360 PHYSIOLOGICAL CHEMISTRY. to the following equation: C6H5COOH + NH2.CH2.COOH = C6H5.CO.NH.CH2.COOH + H20. This acid may be synthetically prepared from benzamid and monochlor-acetic acid, C6H5.CO.NH2 + CH2C1.COOH = C6H6.CO.NH.CH2.COOH + HC1, also in other different ways. Hippuric acid occurs in large amounts in the urine of herbivora, but only in small quantities in that of carnivora. The quantity of hippuric acid eliminated in human urine on a mixed diet is usually less than 1 grm. per 24 hours; as an average it is 0.7 grm. After eating freely of vegetables and fruit, especially such fruit as plums, the quantity may be more than 2 grms. Hippuric acid is also found in the perspiration, blood, suprarenal capsule of oxen, and in the ichthyosis scales. Nothing is positively known in regard to the quantity of hippuric acid in the urine in disease. The Formation of Hippuric Acid in the organism. Benzoic acid and also the substituted benzoic acids are converted into hippuric acid and substituted hippuric acids within the body. Also, those bodies are transformed into hippuric acid which by oxidation (toluol, cinnamic acid, hydrocinnamic acid) or by reduction (quinic acid) are converted into benzoic acid. The question of the origin of hippuric acid is therefore connected with the question of the origin of benozic acid; for the formation of the second component, glycocoll, from the protein substances in the body is without ques- tion. Hippuric acid is found in the urine of starving human beings (SCHULTZEN) and dogs (SALKOWSKI), also in dog's urine after a diet consisting entirely of meat (MEISSI^ER and SHEPARD, SALKOW- SKI, and others). It is evident that the benzoic acid originates in these cases from the proteids. Benzoic acid may indeed be pro- duced outside of the body by the oxidation of the albumins; the benzoic acid produced on a diet consisting entirely of meat seems to be derived from the putrefaction of the proteids in the intestines. Among the products of the putrefaction of albumin outside of the body SALKOWSKI has found phenylpropionic acid, C6H5.CH2.CH2. COOH, which is oxidized in the organism to benzoic acid and eliminated as hippuric acid after combining with glycocoll. Phenyl- propionic acid seems to be formed from the amidophenylpropionic acid, which is prepared only from the plant proteids, and the sup- THE URINE. position that the phenylpropionic acid is produced from tyrosin by' putrefaction in the intestines has not been substantiated by the researches of BAUMANN, SCHOTTEN and BAAS. The importance of putrefaction in the intestines in producing hippuric acid is evi- dent from the fact that after thoroughly disinfecting the intestines of dogs with calomel the hippuric acid disappears from the urine The large quantity of hippuric acid present in the urine of her- bivora is partly explained by the fact that vegetable proteids yield perhaps larger amounts of amidophenylpropionic acid, and partly by the specially active processes of putrefaction going on in the intestines of herbivora. These circumstances do not entirely ex- plain this excess of hippuric acid (SALKOWSKI). The abundant elimination of hippuric acid by herbivora may in part depend on the great amount of aromatic substances in the food of these ani- mals which is converted into benzoic acid. There is hardly any doubt that the hippuric acid in human urine after a mixed diet, and especially after a diet of vegetables and fruits, has in part a similar origin. The kidneys may be considered in dogs as special organs for the synthesis of hippuric acid (SCHMIEDEBERQ and BUNGE). In other animals, as in rabbits, the formation of hippuric acid seems to take place in other organs, such as the liver and muscles. The synthesis of hippuric acid is therefore not exclusively limited to any special organ, though perhaps in some species of animals it may be more abundant in one organ than in another. Properties and reactions of hippuric acid. This acid crystallizes in semi-transparent, milk-white, long, four-sided rhombic prisms or columns, or in needles by rapid crystallization. They dissolve in 600 parts cold water, but more easily in hot water. They are easily soluble in alcohol, but with difficulty in ether. They are more easily soluble (about 12 times) in acetic ether than in ethyl ether. Petroleum ether does not dissolve them. On heating hippuric acid it first melts to an oily liquid which crystallizes on cooling. By continuing the heat it decomposes, pro- ducing a red mass and a sublimate of benzoic acid, with the genera- tion, first, of a peculiar pleasant odor of hay, and then an odor of hydrocyanic acid. Hippuric acid is easily differentiated from ben- 362 PHYSIOLOGICAL CHEMISTRY. zoic acid by this behavior, also by its crystalline form and its in- solubility in petroleum ether. Hippuric acid and benzoic acid both give LUCRE'S reaction, namely, they generate an intense odor of nitrobenzol when evaporated with nitric acid to dryness and when the residue is heated. Hippuric acid forms crystallizable salts, in most cases, with bases. The combinations with alkalies and alkaline earths are soluble in water and alcohol. The silver, copper, and lead salts are soluble with difficulty in water; the iron- oxide salt is insoluble. Hippuric acid is best prepared from the fresh urine of a horse or cow. The urine is boiled a few minutes with an excess of milk of lime. The liquid is filtered while hot, concentrated and then cooled, and the hippuric acid precipitated by the addition of an excess of hydrochloric acid. The crystals are pressed, dissolved in milk of lime by boiling, and treated as above; the hippuric acid is precipitated again from the concentrated filtrate by hydrochloric acid. The crystals are purified by recrystallization and decolorized, when necessary, by animal charcoal. The quantitative estimation of hippuric acid in the urine may be performed by the following method (BuNGE and SCHMIEDE- BERG) : The urine is first made faintly alkaline with soda, evapo- rated nearly to dryness, and the residue thoroughly extracted with strong alcohol. After the evaporation of the alcohol, dissolve in water, acidify with sulphuric acid, and completely extract by agitat- ing (at least five times) with fresh portions of acetic ether. The acetic ether is then repeatedly washed with water, which is removed by means of a separatory funnel, then evaporated at a medium temperature, and the dry residue treated repeatedly with petroleum ether, which dissolves the benzoic acid, oxyacids, fat and phenol, while the hippuric acid remains undissolved. This residue is now dissolved in a little warm water and evaporated at 50°-60° C. to crystallization. The crystals are collected on a small weighed filter. The mother-liquor is repeatedly shaken with acetic ether. This last is removed and evaporated; the residue is added to the above crystals on the filter, dried and weighed. Phenaceturic Acid, C,oHnN03 = C6H5.CH2.CO.NH.CH2.COOH. This acid, which is produced in the animal body by a grouping of the phenyl- acetic acid, C6H5.CH2.COOH, formed by the putrefaction of the proteids with glycocoll, has been prepared from horse's urine by SALKOWSKI, but it probably also occurs in human urine. Benzoic Acid, C7H6O2 or C8HB.COOH, is found in rabbit's urine and some- times, though in small amounts, in dog's urine (WEYL and y. ANBEP). Ac- cording to JAARSVELD and STOKVIS and to KRONECKEK, it is also found in human urine in diseases of the kidneys. The occurrence of benzoic acid in THE URINE. 363 the uriue seems to be due to a fermentative decomposition of hippuric acid. Such a decomposition may very easily occur in an alkaline urine or one con- . taming albumin (VAN DE VELDE and STOKVIB). In certain animals — pigs and dogs— the kidneys, according to SCHMIEDEBERG and MINKOWSKI, contain a special enzyme, SCHMIEDEBERG'S histozym, which splits the hippuric acid with the separation of benzoic acid. Ethereal Sulphuric Acids. Phenol, whose mother-substance is considered to be tyrosin, is produced by the putrefaction of albumin in the intestines ; indol and skatol are also produced. The phenol and the two last-mentioned bodies, after they have been oxidized into indoxyl and skatoxyl, pass into the urine as ethereal sulphuric acids after uniting with sulphuric acid. The most important of these ethereal acids are phenol- and cresol-sulpliuric acid — which were formerly called phenol-forming substance — indoxyl- and skatoxyl-sulplmric acid. To this group belong also the pyrocate- chin-sulphuric acid, which only occurs in very small amounts in human urine, and hydrochinon-sulpUuric acid, which appears in the urine after poisoning with phenol, and perhaps under physio- logical conditions other ethereal acids occur which have not been isolated. The ethereal sulphuric acids of the urine were discovered and specially studied by BATJMANN. The quantity of these acids in the urine is small. The quantity of grouped sulphuric acid per twenty-four hours is on an average 0.25 grm., but varies between 0.094 and 0.620 grm. The relationship of the sulphate-sulphuric acid A to the grouped sulphuric acid B in health is on an average as 10 : 1, but varies from 6 : 1 and 15 : 1. After taking phenol, also after increased putrefaction within the organism, this relation- ship may be essentially changed by an increased elimination of ethereal sulphuric acids. The urine of the horse is considerably richer in ethereal sulphuric acids than human urine. Phenol- and p-Cresol-sulphuric Acid, C6HB.O.S02.OH and C7H,.O.S02.OH. These acids are found as alkali salts in human urine, in which also orthocresol has been detected. The quantity of cresol-sulphuric acid is considerably greater than phenol-sul- phuric acid. In the quantitative estimation the phenols set free from the two ethereal acids are determined together as tribrom- phenol. The quantity of phenol which is separated from the ethereal sulphuric acids of the urine amounts to 17-51 milligrammes in the twenty-four hours (MuNK). After a vegetable diet the 364 PHYSIOLOGICAL CHEMISTRY. amount is greater than after a meat diet. After taking carbolic acid, which is in great part converted by synthesis within the or- ganism into phenol-ethereal sulphuric acid, pyrocatechin- and hydrochinon-sulphuric acid, and also when the amount of sulphuric acid is not sufficient to combine with the phenol forming phenyl- glycuronic acid, the amount of phenol and ethereal sulphuric acids in the urine is considerably increased at the expense of the sulphate- sulphuric acid. An increased elimination of phenol-sulphuric acid occurs in active putrefaction in the intestines with stoppage of the con- tents of the intestine, as in ileus, diffused peritonitis with atonia of the intestine, or tuberculous enteritis, but not in simple ob- struction. The elimination is also increased by the absorption of the products of putrefaction from purulent wounds or abscesses. An increased elimination of phenol has been observed in a few other cases of diseased conditions of the body. The alkali salts of phenol- and cresol-sulphuric acids crystallize in white plates, similar to mother-of-pearl, which are rather freely soluble in water. They are soluble in boiling alcohol, but only slightly soluble in cold. On boiling with dilute mineral acids they are decomposed into sulphuric acid and the corresponding phenol. Phenol-sulphuric acids have been synthetically prepared by BAUMANN from potassium pyrosulphate and phenol- or p-cresol-po- tassium. For the method of their preparation from urine, which is rather complicated, the reader is referred to other text-books. The quantitative estimation of these ethereal sulphuric acids is done by BAUMANN and BRIEGER by determining the amount of phenol which may be separated from the urine as tribromphenol. In this determination, when the urine is not specially rich in phenol, about one fourth of the total quantity in the twenty-four hours is used; it is acidified with concentrated hydrochloric acid — 5 c. c. for every 100 c. c. of urine — and distilled until a portion of the distillate does not give the slighest reaction for phenol with MILLON'S re- agent or with bromine-water. The distillate is now carefully neu- tralized with soda solution (which combines with the benzoic acid, etc.) and again distilled until a portion of the distillate is free from phenol, as shown by the above-mentioned reagents. This distillate is treated with bromine-water until a permanent yellow color is produced, and then allowed to stand for about twenty-four hours in the cold; the crystalline precipitate then is collected on a small THE URINE. 365 weighed filter, washed with dilute bromine-water, dried over sul- phuric acid without the use of a vacuum and weighed (331 parts tribromphenol correspond to 94 parts phenol). Paracresol is gradu- ally converted into tribromphenol by this treatment with bromine- water. The methods for the separate determination of the coupled sulphuric acid and the sulphate-sulphuric acid will be spoken of later in connection with the determination of the sulphuric acid of the urine. Pyrocatechin-sulphuric Acid (and PYROCATECHIN). This acid was first found in horses' urine iii rather large quantities by BAUMANN. It only occurs in human urine in the very smallest quantities and perhaps not constantly, but it occurs abundantly in the urine after taking phenol, pyrocatechin, or protocatechinic acid. On an exclusive meat diet this acid does not occur in the urine, and it therefore originates from the vegetable food. It probably originates from the protocatechuic acid, which, according to PREUSSE, passes in part into the urine as pyrocatechin-sulphuric acid. This acid may also perhaps depend on oxidation of phenol within the organism (BAUMANN and PREUSSE). Pyrocatechin, or o-DioxYBENZOL, C6H4(OH)a, was first observed in the urine of a child (EPSTEIN and J. MULLER). The reducible body ALCAPTON, first found by BODEKER in human urine and which was considered for a long time as identical with pyrocatechin, is UROLEUCIC ACID, according to KIRK. Pyrocatechiu crystallizes in prisms which are soluble in alcohol, ether, and water. It melts at 102°-104° 0. and sublimes in shining plates. The watery solution becomes green, brown, and ultimately black in the presence of alkali and the oxygen of the air. If very dilute ferric chloride is treated with tartaric acid and then made alkaline with ammonia and this added to a watery solution of pyrocatechin, we obtain a violet or cherry-red liquid which becomes green by saturating with acetic acid. Pyrocatechin is precipitated by lead acetate. It reduces an ammoniacal silver solution at the ordinary temperature and reduces alkaline copper-oxide solutions with heat, but does not reduce bismuth oxide. A urine containing pyrocatechin, if exposed to the air, especially when alkaline, quickly becomes dark and reduces alkaline copper solutions with heat. In detecting pyrocatechin in the urine it is concentrated when necessary, filtered, boiled with the addition of sulphuric acid to remove the phenols, and repeatedly shaken after cooling with ether. The ether is distilled from the several ethereal extracts, the residue neutralized with barium carbonate and shaken again with ether. The pyrocatechin which remains after evaporating the ether may be purified by recrystallization from benzol. Hydroctonon, or P-DIOXYBENZOL, CeH^OEDa, often occurs in the urine after the use of phenol (BAUMANN and PREUSSE). The dark color which cer- tain urines, so-called " carbolic urines," take in the air is due to decomposition products. Hydrochinon does not occur as a normal constituent of urine, but after the administration of hydrochinon ; according to v. MERINO and LEWIN, it passes into the urine of rabbits as ethereal sulphuric acid, as a decomposition product of arbutin. Hydrochinon forms rhombical crystals which are readily soluble in water, alcohol, and ether. It melts at 169° C. Like pyrocatechin. it easily reduces metallic oxides. It acts like pyrocatechin with alkalies, but is not precipitated with lead acetate. It is oxidized into chinon by ferric chloride and other oxidizing agents, and chinon is detected by its peculiar odor. Hydrochinon- sulphuric acid is detected in the urine by the same methods as pyrocatechin- sulphuric acid. 366 PHYSIOLOGICAL CHEMISTRY. Indoxyl-sulphuric acid, C^NSO^ or C8H6N.O.S02.OH, also called URIKE IXDICAN, formerly called UROXANTHIN (HELLER), occurs as alkali-salt in the urine. This compound is the mother- substance of a great part of the indigo of the urine. The quantity of indigo which can be separated from the urine is considered as a measure of the quantity of indoxyl-sulphuric acid (and indoxyl- glycuronic acid) contained in the urine. This amount, according to JAFFE, for man is 5-20 milligrammes per 24 hours. Horse's urine contains about 25 times as much indigo-forming substance as human urine. Indoxyl-sulphuric acid is derived, as above mentioned (page 21 6), from indol, which is first oxidized in the body into indoxyl and then is coupled with sulphuric acid. After subcutaneous injection of indol the elimination of indican is considerably increased (JAFFE, BAUMANN and BRIEGER). It is also increased by the introduction of orthonitrophenylpropiolic acid in the organism of animals (G-. HOPPE-SEYLER). Indol is formed by the putrefaction of proteids, and it is therefore easy to understand why the quantity of indoxyl-sulphuric acid is greater with a meat than with a vege- table diet. The putrefaction of secretions rich in albumin in the intestine explains also the occurrence of indican in the urine during starvation. Gelatine, on the contrary, does not increase the elimination of indican. An abnormally-increased elimination of indican occurs in such diseases as obstruct the small intestine, causing an increased putrefaction, thus producing an abundant formation of indol. Such an increased elimination of indican occurs on tying the small intestine of a dog, but not the large intestine (JAFFE). The simple obstruction of the human colon does not increase the indican in the urine. The obstruction of the large intestine may, when it causes a considerable disturbance in the motion of the contents of the upper ileum, produce an increased elimination of indican. Like the putrefaction in the intestine, the putrefaction of proteids in other organs and tissues of the body may cause an increase in the indican of the urine. An increased elimination of indican has been observed in many diseases, such as in ileus, cholera, acute general peritonitis, abscess, and carcinoma of the stomach, intestinal catarrh, multiple lym- THE URINE. 367 pnoma, fetid bronchitis, ichorous pleural exudations, diabetes mellitus, and others. The increase of indican in the urine observed in consumption and inanition depends probably upon the disturbed digestion. In increased elimination of indican the elimination of phenol is also increased ; a urine rich in phenol is, on the contrary, not always rich in indican. The potassium-salt of indoxyl-sulphuric acid which was pre- pared by BAUMANN and BRIEGER from the urine of a dog fed on indol, crystallizes in colorless, shining plates or leaves which are easily soluble in water but less readily in alcohol. It is split by mineral acids into sulphuric acid and indoxyl. The latter without access of air passes into a red compound, indoxyl-red, but in the presence of oxidizing reagents is converted into indigo-blue : 2C8H7NO + 20 = C16H10N202 -f 2H80. The detection of indican is based on this last fact. For the rather complicated preparation of indoxyl-sulphuric acid as potassium-salt from urine the reader is referred to other text-books. For the detection of indican in urine in ordinary cases the following method of JAFFE, which also serve as an approximate test for the quantity of indican, is sufficient. JAFFE'S Indican Test. 20 c. c. of urine are treated in a glass with 2-3 c. c. chloroform and mixed with an equal volume of concentrated hydrochloric acid. Immediately after a concentrated chloride-of-lime solution or a \% potassium-permanganate solution is added drop by drop, and after each drop the mixture is thoroughly shaken. The chloroform is gradually colored faintly or strongly blue. An excess of oxidizing reagent, especially chloride of lime, interferes with the reaction and must therefore be avoided. The test is repeated with somewhat varying amounts of oxidizing material until a point is found at which the maximum coloration of the chloroform takes place. From the intensity of the color the quantity of indigo is determined. An exact determination of the amount of indigo in urine is very rarely made. The methods suggested for this purpose are very complicated, and even then they are not quite accurate; therefore the reader is referred to other text-books for their description. Indol seems also to pass into the urine as a glycuronic acid, indoxyl- gly cur onic acid (SCHMIEDEBERG). Such an acid has been found in the urine of animals after the administration of the sodium-salt of o-nitrophenylpropiolic acid (,G. HOPPE-SEYLER). 368 PHYSIOLOGICAL CHEMISTRY. Skatoxyl-sulphuric Acid, C9H9lSrS04 or 09H8.N.O.S02.OH. The potassium-salt of this acid seems to occur generally in human urine as a chromogen, which yields a red or violet coloring matter on de- composing with strong acids, and an oxidizing reagent. This salt has been prepared by OTTO from diabetic human urine. Little is known of the quantity of this skatolchromogen, to which probably also the skatoxyl-glycuronic acid must be counted, under physiol- ogical and pathological conditions. Skatoxyl-sulphuric acid originates from skatol formed by putre- faction in the intestine, which is coupled with sulphuric acid after oxidation into skatoxyl. That skatol introduced into the body passes partly as an ethereal sulphuric acid into the urine has been shown by BRIEGER. Indol and skatol act differently, at least in dogs; indol producing a considerable amount of ethereal sulphuric acid, while skatol only gives a small quantity (MESTER). Skatol seems partly to pass into the urine as a skatoxyl-glycuronic acid. The potassium-salt of skatoxyl-sulphuric acid is crystalline; it dissolves in water, but with difficulty in alcohol. A watery solution becomes deep violet with ferric chloride, and red with concentrated nitric acid. The salt is decomposed by concentrated hydrochloric acid with the separation of a red precipitate. The nature of this red coloring matter produced by the decomposition of skatoxyl- sulphuric acid is not well known; neither is the relationship exist- ing between this and other red coloring matters in the urine known. On distillation with zinc-dust the skatol coloring matter yields skatol. Urines containing skatoxyl are colored dark red to violet by JAFFE'S indican test even after the addition of hydrochloric acid; with nitric acid they are colored cherry-red, and on warming with ferric chloride and hydrochloric acid red. The coloring matter which yields skatol with zinc-dust may be removed from the urine by ether. Urines rich in skatoxyl darken when allowed to stand, and may become reddish, violet, or nearly black. SALKOWSKI has shown that the occurrence of skatol-carbonic acid, C9H8. N.COOH, in normal urine is probable. This is also a putrefaction product. Aromatic Oxyacids. In the putrefaction of proteids in the intestine, paraoxyplienyl-acetic acid, C6H4(OH).CH2COOH, and THE URINE. 369 paraoxyphenyi-propionic acid, C6H4(OH).C2H4.COOH, are formed from tyrosin as intermediate steps, and these pass unchanged into the urine. They were first detected by BAUMANN. The quantity of these acids is usually very small. They are increased by the same circumstances as phenol, especially in acute phosphorus-poi- soning, in which the increase is considerable. In acute atrophy of the liver another oxyacid, oxytnandel-acid, has been found in the urine (SCHULTZEN and RIESS). The above two acids are soluble in ether. On warming with MILLON'S reagent they give a beautiful red color. To detect the presence of these oxy- acids proceed in the following way (BAUMANN) : Warm the urine for a while on the water-bath with hydrochloric acid, in order to drive off the volatile phenols. After cooling shake three times with ether, and then shake the ethereal extracts with dilute soda solution, which dissolves the oxyacids, while the residue of the phenols soluble in ether remains. The alkaline solution of the oxyacids is now faintly acidified with sulphuric acid, shaken again with ether, the ether removed and allow to evaporate, the residue dissolved in a little water, and the solution tested with MILLON'S reagent. The two oxyacids are best differentiated by their different melting-points. The reader is referred to other works for the method of isolating and separating these two oxyacids. Uroleucic Acid, C9H,oO6. This acid in a pure state was first prepared by MARSHALL from urine, but named and specially studied by KIRK. In an im- pure state it forms the reducible substance ALCAPTON discovered by BOEDE- KER. This acid is especially found in children's urine. Such urine reduces FEHLING'S reagent, but not an alkaline bismuth or picric-acid solution. It is fermentable, optically inactive, and is colored deep brown in the air, espe- cially in an alkaline solution. In these respects it differs from a urine con- taining sugar. Urinary Coloring Matters and Chromogens. The yellow color of normal urine depends apparently upon several coloring matters (VIERORDT) which have not been isolated and studied. Besides these bodies, UROBILIN sometimes occurs in fresh normal urine, but by no means always. Instead of urobilin, normal urine often con- tains a mother-substance of the same, a chromogen or UROBILINOGEN, from which the urobilin is gradually formed by oxidation on allow- ing the urine to stand exposed to the air (JAFFE, STOKVIS, DISQUE, and others). Besides this chromogen, urine contains various other bodies from which coloring matters may be produced by the action of chemical agents. Humous substances (perhaps in part from the carbohydrates of the urine) may be formed by the action of acids (v. UDRANSZKY and HOPPE-SEYLER) without regard to the fact that such substances may sometimes originate from the reagents used, as from impure amyl-alcohol (v. UDRANSZKY and HOPPE- 370 PHYSIOLOGICAL CHEMISTRY. SEYLER). To these humin bodies developed by the action of acid in normal urine when exposed to the air must be added the UROPHAIN of HELLER, the various UROMELANINS, and other bodies described by different investigators (PLOS'Z, THUDICHUM, SCHUNCK). Indigo-blue (UROGLAUCIN of HELLER, UROCYANIN, CYANURIN, and other coloring matters of older investigators) is split off from the indoxyl-sulphuric acid or indoxyl-glycuronic acid. Red coloring matters may be formed from the coupled indoxyl and skatoxyl acids, and UROHODIN (HELLER), URORUBIN (PLOS'Z), UROH^IMATIN (HARLEY), and perhaps also UROROSEIN (NENCKI and SIEBER) probably have such an origin. We cannot enter into too many details of the different coloring matters obtained as decomposition products from normal urine; and as the preformed physiological coloring matters of urine have not been closely studied, we can only discuss the most carefully- investigated urinary pigment, urobilin. TTrobilin was first prepared from urine by JAFFE. This color- ing matter occurs in urine especially in fevers, and it is therefore designated FEBRILE UROBILIN by MACMUNN. The urobilin occurring in normal urine is somewhat different from an optical standpoint from the above, and is called NORMAL UROBILIN by MAcMuNN. As above stated, a mother-substance of urobilin, a UROBILINOGEN, occurs in the urine, from which urobilin is pro- duced by the action of the air. Many investigators claim that urobilin is identical with hydro- bilirubin (MALY) and corresponds to the composition C32H40lSr407. Also, that urobilin is formed by a reduction- of bilirubin in the intestine. The correctness of this view is disputed by others (MAcMuNN, LE NOBEL). According to MAcMuNN, hydrobiliru- bin and the urinary urobilin are not identical bodies, because he obtained normal urobilin by the action of peroxide of hydrogen upon a solution of haematin in alcohol containing sulphuric acid. Coloring matters similar to urobilin, though not identical, have been obtained from the biliary and from the blood coloring mat- ters. Besides the hydrobilirubin prepared by MALY from bilirubin, STOKVIS obtained a choletelin from a biliary pigment, cholecyanin, by the action of zinc chloride and tincture of iodine, or by boiling THE URINE. 371 with a little lead peroxide. This choletelin acts like urobiliu, but that obtained from bilirubiu by the action of nitric acid does not. 'Bodies similar to urobilin have also been obtained by HOPPE- SEYLER, by the reduction of haematin and haemoglobin with zinc and hydrochloric acid; by LE NOBEL, by treating an acid-alcoholic or alkaline solution of haematoporphyrin with tin or zinc; and lastly by NENCKI and SIEBEE, by treating haematoporphyrin with zinc and hydrochloric acid. From the observations of LE NOBEL and NEDTCKI and SIEBER it follows that these coloring matters arti- ficially prepared from the blood-coloring matters are not identical, even though they are closely related from an optical standpoint. It must be left undecided whether these bodies are identical with each other or with the urinary urobilin, or if the observed difference is only due to a contamination with other bodies. Because of our imperfect knowledge of the urobilin of the urine and the UROBILINOIDIN (this name has been given by LE NOBEL to the substance artificially prepared by him) it is difficult to say anything positive in regard to the occurrence of urobilin in the urine in disease. During the absorption of large blood extravasa- tions, as also in diseases connected with destruction of the blood- corpuscles or of the appearance of methaemoglobin in the blood- plasma, the urine becomes dark in color, which generally depends upon an increased elimination of urobilin. The question whether it depends on an increased elimination of urinary urobilin or, as is more probable, upon the urobilinoidin produced from the blood- coloring matters is still doubtful. In icterus the elimination of urobilin is often increased, and indeed cases occur in which the urobilin is almost the only coloring matter which can be detected in icteric urines (UROBILINICTERUS). In these cases we are probably dealing with a urobilinoid substance produced from the bile-coloring matters. The urobilin obtained from a fever urine is, according to JAFFE, amorphous, red, dingy-red, or reddish yellow, according to the method of preparation. It dissolves easily in alcohol, amyl- alcohol, and chloroform, but less readily in ether. It is less soluble in water, but the solubility is augmented in the presence of a neutral salt. It may be precipitated from a solution saturated 372 PHYSIOLOGICAL CHEMISTRY. with ammonium sulphate by the addition of sulphuric acid (MEHY). It is soluble in alkalies and is incompletely precipitated from the alkaline solution by the addition of acid. It is partly dissolved by chloroform from an acid (watery-alcoholic) solution ; alkali solutions remove the urobilin from the chloroform. The alkaline solutions of urobilin give insoluble combinations with salts of the heavy metals, such as zinc and lead. Urobilin does not give GMELIN'S test for bile-pigments. Neutral alcoholic urobilin solutions are in strong concentration brownish yellow, in great dilution yellow or rose-colored. They have a strong green fluorescence. The acid-alcoholic solutions are, according to concentration, brown, reddish yellow, or rose-red. They are not fluorescent, but show a faint absorption-band, y, between b and F, which borders on F, or in greater concentration extends over F. The alkaline solutions are, according to concen- tration, brownish yellow, yellow, or (the ammoniacal) yellowish green. If some zinc-chloride solution is added to an ammoniacal solution, it becomes red and shows a beautiful green fluorescence. This solution, as also that made alkaline with fixed alkalies, shows a darker and more sharply-defined band, tf, almost midway between b and F. The urobilin obtained by MAcMuNN according to other methods, and that obtained by JAFFE, differ from each other mainly in the following: A solution of normal urobilin becomes deeper red with soda, while the febrile urobilin becomes yellow. The band y of the normal urobilin disappears on the addition of alkali, while the corresponding band of the febrile moves towards the left. The ethereal solution of febrile urobilin shows two faint absorption-bands on each side of D which are not to be seen in the watery solution nor in the urine. Febrile urobilin is a brownish- red and the normal a yellowish-brown powder. Febrile urobilin is, according to MAcMuNK, converted into normal urobilin by po- tassium permanganate. In preparing urobilin from normal urine, precipitate the urine with basic lead acetate (JAFFE), wash the precipitate with water, dry at the ordinary temperature, then boil it with alcohol, and decompose it when cold with alcohol containing sulphuric acid. The filtered alcoholic solution is diluted with water, saturated with THE URINE. 373 ammonia, and then treated with zinc-chloride solution. This new .precipitate is washed free from chlorine with water, boiled with alcohol, dried, dissolved in ammonia, and this solution precipitated with sugar of lead. This precipitate, which is washed with water and boiled with alcohol, is decomposed by alcohol containing sul- phuric acid, the filtered alcoholic solution is mixed with f vol. chloroform, diluted with water, and shaken repeatedly, but not too energetically. The urobilin is taken up by the chloroform. This last is washed once or twice with a little water and then filtered, leaving the urobilin, which is purified from a contaminating red coloring matter by means of ether. According to JAFFE, the coloring matter can be directly precipitated from a fever urine rich in urobiliu by ammonia and zinc chloride, and this precipi- tate treated as above. MEHY faintly acidities the urine with sulphuric acid (1-2 grms. per litre), then saturates with ammonium sulphate, washes the pre- cipitate on a filler with an acidified ammonium-sulphate solution, presses the filter, and extracts the coloring matter with absolute alcohol at a gentle heat after the addition of a few drops of ammonia. MACMUNN precipitates the urine with sugar of lead and basic lead acetate, decomposes the precipitate with acidified alcohol, dilutes the solution with water, shakes with chloroform, evaporates this last, and dissolves the residue repeatedly with chloroform. The method of preparation, according to MACMUNN, is the same for both urobilins, the normal and the febrile. The color of the acid or alkaline solution, the beautiful fluores- cence of the ammoniacal solution treated with zinc chloride, and the absorption-bands of the spectrum, all serve as means of detect- ing urobilin. In fever urines the urobilin may be detected directly or after the addition of ammonia and zinc chloride by its spectrum. It may also be detected sometimes in normal urine directly or after the urine has stood exposed to the air until the chromogen has been converted into urobilin. If it cannot be detected by means of the spectroscope, then the urine may be treated with a mineral acid and shaken with ether. The ethereal solution may be, directly or after concentration, tested with the spectroscope. It is often bet- ter to dissolve the residue, after the evaporation of the ether, in absolute alcohol, and use this for the spectroscopic investigation. According to SALKOWSKI, the urobilin may be directly extracted by gently shaking with ether free from alcohol. If the urobilin cannot be detected by the above-described methods, then precipi- tate the urine with basic lead acetate, decompose the precipitate with acidified alcohol, test this solution or extract the coloring matter by diluting with water and shaking with chloroform. The urochrom (THUDICHUM) seems to be a mixture of several bodies. Uroerythrin is that coloring matter which often colors the urinary sediment (sedimentum lateritium) beautifully red. It occurs especially in fevers and other diseases, but it is not found in the urine of perfectly healthy persons. 374 PHYSIOLOGICAL CHEMISTRY. Volatile fatty acids, such as formic acid, acetic acid, and perhaps also butyric acid, occur under normal conditions in human urine (v. JAKSCH), also in that of dogs and herbivora (SCHOTTEN). The acids poorest in carbon, formic acid, and acetic acid are more constant in the body than those richer in carbon, and therefore the relatively greater part pass unchanged into the urine (SCHOTTEN). Normal human urine contains besides these bodies others which yield acetic acid when oxidized by potassium dichromate and sulphuric acid (V. JAKSCH). The quantity of volatile fatty acids in normal urine is, according to v. JAKSCH, 0.008-0.009 grm. per 24 hours, and according to v. ROBITANSKY, 0.054 grm. The quantity is increased by exclusive farinaceous food, also in fever and in certain diseases of the liver (v. JAKSCH). It is also increased in leucaemia and in many cases of diabetes (v. JAKSCH). Large amounts of volatile fatty acids are produced in alkaline fermentation of the urine, and the quantity is 15-16 times as large as in normal urine (SALKOWSKI). Paralactic Acid. It is claimed that this acid occurs in the urine of healthy persons after very fatiguing marches (COLASANTI and MOSCATELLI). It is found in larger amounts in the urine in acute phosphorus poisoning or acute yellow atrophy of the liver (Scnui/rzEN and RIESS), also in osteomalacia (MoRS and MUCK). After the extirpation of the liver of birds it is found in large quantities in their urine (MINKOWSKI). Glycero-pJiosphoric acid occurs as traces in the urine, and it is probably a decomposition product of lecithin. The occurrence of succinic acid in normal urine is the subject of discussion. Carbohydrates and Reducing Substances in the Urine. The occurrence of grape-sugar as traces in normal urine is highly prob- able, as the investigations of BRUCKE, ABELES and v. UDRANSZKI show. The last has also shown the habitual occurrence of carbo- hydrates in the urine, and their presence has been positively proved by the investigations of BAUMANN and WEDENSKI. Besides this, the urine contains traces of a carbohydrate similar to dextrin (animal gum) (LANDWEHR, WEDESTSKI). Besides traces of sugar and the previously-mentioned reducing substances, uric acid and creatinin, the urine contains still other reducing substances. These last are probably (FLUCKIGER) coupled combinations of glycuronic acid, C6H1007, which closely resembles sugar. The reducing power of normal urine corresponds according to FLUCKIGER to 1.5-2.5 p. m. grape-sugar, according to SALKOWSKI 4.08, according to MUNK an average of 3.0, and according to WORM MULLER about 4.0 p. m. Glycuronic Acid, C6H1007 or CHO.(CH.OH),.COOH. This acid may be converted into saccharic acid, C6H1008, by the action of bromine (THIERFELDER), and it seems to occupy an intermediate position between this acid and gluconic acid, C6H1207 , obtained by the oxidation of glucose or cane-sugar with chlorine or bromine. Glycuronic acid probably only occurs normally in very small quanti- THE URINE. 375 ties in human urine as coupled combinations with indoxyl, skatoxyl, and phenols. It is also found in the artists' color " jaune indien," which contains the magnesium -salt of euxanthonic acid. On heat- ing this acid with water to 120°-125° C. it splits into euxanthin and glycuronic acid. This acid may pass into the urine in larger quantities as coupled glycuronic acids after the administration of various medicines or other substances (see below). Thus after the administration of chloral hydrate, naphthalin, camphor, and turpentine, respectively, urochloralic acid, naphthol-glycuronic acid, campho-glycuronic acid, and terpen-glycuroriic acid appear in the urine. The coupled glycuronic acids turn the plane of polarization to the left, while glycuronic acid itself is dextro-gyrate. With the absorption of water they may split into glycuronic acid and other coupled bodies. A few of the coupled glycuronic acids, such as the urochloralic acid, reduce copper oxide and certain other metallic oxides in al- kaline solution, and therefore they may interfere with the detection of sugar in the urine. Glycuronic acid is not crystalline, but is obtained only as a syrup. It dissolves in alcohol and is easily soluble in water. If the watery solution is boiled for an hour, the acid is in part (20$) converted into the anhydride GLYCUROtf,C6H806, which is crystalline, soluble in water but insoluble in alcohol. The potassium-salt of this acid crystallizes in fine needles. The neutral barium-salt is amorphous, soluble in water, but is precipitated by alcohol. If a concentrated solution of the acid is saturated with barium hydrate, the basic ba- rium-salt separates. The neutral lead-salt is soluble in water, but the basic salt is, on the contrary, insoluble. The acid is dextro- gyrate, reduces copper, silver, and bismuth salts. It gives a crys- talline combination with phenylhydrazin. Glycuronic acid may be prepared from urochloralic acid or cam- pho-glycuronic acid by boiling with a mineral acid. It may be prepared more easily by heating euxanthonic acid with water in PAPIN'S digestor to 120b-125° C. for an hour and evaporating the watery solution at+40° C. The anhydride which crystallizes grad- ually is removed, the mother-liquor diluted with water and boiled for a time to convert a second portion of acid into anhydride, and then evaporated at about -j- 40° C. This is continued until nearly 376 PHYSIOLOGICAL CHEMISTRY. all the acid is converted into anhydride. The anhydride may then be further purified. Organic combinations containing sulphur of unknown kind, which may in small part consist of sulphocyanides, 0.04 (GSCHEIDLEN) — 0.11 p. m. (J. MUNK), cystin, or bodies related to it, and protein bodies, are found in human as well as in animal urines. The sulphur of these mostly unknown combina- tions has been called " neutral," to differentiate it from the "acid " sulphur of the sulphate and ethereal-sulphuric acids (SALKOWSEJ). The neutral sul- phur in normal urine as determined by SALKOWSKI is 15$, by STADTHAGEN 13.3-14.5$, and by LEPINE 20$ of the total sulphur. An increase in the quan- tity of neutral sulphur has been observed in icterus (LEPINE), and in cystin- uria (STADTHAGEN). The total quantity of sulphur in the urine is determined by fusing the solid urinary residue with saltpetre and caustic alkali. The quantity of neutral sulphur is determined as the difference between the total sulphur and the sul- phur of the sulphate and ethereal sulphuric acids. Sulphuretted hydrogen occurs in urine only under abnormal conditions or as a decomposition product. Sulphuretted hydrogen may be produced from the neutral sulphur of the organic substances of the urine by the action of certain bacteria (FR. MULLER, SALKOWSKI). Other investigators (ROSENHEIM and GUTZMANN) have given hyposulphites as the source of the sulphuretted hydrogen. The occurrence of hyposulphites in normal human urine, which is asserted by HEFFTER, is disputed by SALKOWSKI. In cat's and dog's urine the hyposulphites are, on the contrary, constant. Organic combinations containing phosphorus (glycero-phosphoric acid, etc.), which yield phosphoric acid on fusing with saltpetre and caustic alkali, are also found in urine (ZULZER, LEPINE, EYRONNET, and AUBERT). Enzymes of various kinds have been isolated from the urine. Among these we may mention pepsin (BRUCKE and others), diastatic enzyme (COHNHEIM and others), and rennet (GRUTZNER, HOLOVTSCHINER, HELWES). The occurrence of trypsin in the urine is doubtful. Substances similar to mucin (nucleoalbumin ?) from the urinary passages and the bladder are generally present in the urine, though in very small amounts. According to several investigators (LEUBE, HOFMEISTER, POSNER), normal human urine also contains traces of albumin. Ptomaines and leucomaines or poisonous substances of an unknown kind, which are often described as alkaloidal substances, occur in normal urine (PoucHET, BOUCHARD, ADUCCO, and others). Under pathological conditions the quantity of these substances may be increased (BOUCHARD, LEPINE and GUERIN, VILLIERS, and others). Within the last few years the poisonous properties of urine have been the subject of more thorough investigation, especially by BOUCHARD. He found that the night urine is less poisonous than the day urine, and that the poisonous constituents of the day and night urines have not the same action. BAUMANN and v. UDRANSZKY have shown that ptomaines may occur in the urine under pathological conditions. They demonstrated the presence of the two ptomaines discovered and first isolated by BRIEGER— putrescine, GMImNa (tetramethylendiamin), and cadaverin, CeHiJSj (pentamethylendiamin)— in the urine of a patient suffering from cj^stinuria and catarrh of the bladder. BRIEGER, v. UDRANSZKY and BAUMANN and STADTHAGEN have shown that not only these but other diarnins occur under physiological conditions. The occurrence in normal urine of any "urine poison" is denied by certain inves- tigators, such as FELTZ and RITTER and STADTHAGEN. The poisonous action of the urine, according to them, is due in great part to the potassium THE URINE. 377 Many substances have been observed in animal urine which are not found in human urine. To these belong : cynurenic acid, Ci0H7NO3 , occurring in dog's urine and which is an oxychinolin carbonic acid ; urocanic acid (JAPPB), first found in dog's urine ; damaluric acid and damolic acid (according to SCHOTTEN, probably a mixture of benzoic acid with volatile fatty acids), obtained by the distillation of cow's urine ; and lastly the lithuric acid, found in the urinary concrements of certain animals. III. Inorganic Constituents of Urine. Chlorides. The chlorine occurring in urine is undoubtedly com- bined with the bases contained in this excretion; the chief part is combined with sodium. In accordance with this, the amount of chlorine in the urine is generally expressed as NaCl. The amount of chlorine combinations in the urine is subject to considerable variation. In general the quantity for a healthy grown person on a mixed diet is 10-15 grms. NaCl per 24 hours. The quantity of common salt in the urine depends chiefly upon the quantity of salt in the food, with which the elimination of chlorine increases and decreases. Abundant drinking of water also increases the elimination of chlorine, which is greater during activity than during rest (during night). Certain organic chlorine combinations, such as chloroform, may increase the elimination of inorganic chlo- rides by the urine (ZELLEB, MYLITJS, KAST). In diarrhoea, in quick formation of large transudations and exudations, also in specially-marked cases of acute febrile diseases, at the time of the crisis, the elimination of common salt is signifi- cantly decreased. The elimination is abnormally increased in the first days after the crisis and during the absorption of extensive exudations. A diminished elimination of chlorine is found in acute and chronic diseases of the kidneys accompanied with albu- minuria. In chronic diseases the elimination of chlorine in general keeps pace with the nutritive condition of the body and the activity of the secretion of the urine. As a rule the chlorine is diminished in chronic diseases. The quantitative estimation of chlorine in urine is most simply performed by titration with silver-nitrate solution. The urine must not contain either albumin (which if present must be re- moved by coagulation) or iodine or bromine compounds. In the presence of bromides or iodides evaporate a measured quantity of the urine to dryuess, fuse the residue with saltpetre and soda, dissolve the 378 PHYSIOLOGICAL CHEMISTRY. fused mass in water, and remove the iodine or bromide by the addition of dilute sulphuric acid and some nitrite, and thoroughly shake with carbon disulphide. The liquid thus obtained may now be titrated with silver nitrate according to VOLHARD'S method. The quantity of bromide or iodide is cal- culated as the difference between the quantity of silver-nitrate solution used for the titration of the solution of the fused mass and the quantity used for the corresponding volume of the original urine. The otherwise beautiful titration method of MOHR, according to which we titrate with silver nitrate in neutral liquids, using neutral potassium chromate as an indicator, cannot be used directly on the urine in careful work. Organic urinary constituents are also precipitated by the silver-salt, and the results are therefore somewhat high for the chlorine. If we wish to use this method, the organic urinary constituents must first be destroyed. For this purpose evaporate to dryness 5-10 c.c. of the urine, after the addi- tion of 1 grm. of chlorine-free soda and 1-2 grms. chlorine-free saltpetre, and carefully fuse. The mass is dissolved in water, acidified faintly with nitric acid, and then neutralized exactly with pure lime carbonate. This neutral solution is used for the titration. The silver-nitrate solution may be a — solution. It is often made of such a strength that each c. c. corresponds to 0.006 grm. Cl or 0.01 grm. NaCl. This last-mentioned solution contains 29.075 grms. AgN03 in 1 litre. VOLHARD'S METHOD. Instead of the preceding determination, VOLHARD'S method, which can be performed directly on the urine, may be employed. The principle is as follows : All the chlorine from the urine acidified with nitric acid is precipitated by an excess of silver nitrate, filtered, and in a measured part the quantity of silver added in excess is determined by means of a sulphocyanide solution. This excess of silver is completely precipitated by the sulphocyanide, and a solution of some ferric salt, which, as is well known, gives a blood-red reaction with the smallest quantity of sulphocyanide, is used as an indicator. We require the following solutions for this titration : 1. A silver- nitrate solution which contains 29.075 grms. AgN03 per litre and of which each c. c. corresponds to 0.01 grm. NaCl or 0.00607 grm. Cl ; 2. A saturated solution at the ordinary temperature of chlorine-free iron alum or ferric sulphate; 3. Chlorine-free nitric acid of a specific gravity of 1.2 ; 4. A potassium sulphocyanide solution which contains 8.3 grms. KCNS per litre, and of which 2 c. c. corresponds to 1 c. c. of the silver-nitrate solution. About 9 grms. of potassium sulphocyanide are dissolved in water and diluted to one litre. The amount of KCNS contained in this solution is determined by the silver-nitrate solution in the following way : Measure exactly 10 c. c. of the silver solution and treat with 5 c. c. of nitric acid and THE URINE. 379 1-2 c. c. of the ferric-salt solution, and dilute with water to about 100 c. c. Now the sulphocyanide solution is added from a burette, constantly stirring, uiitil a permanent faint red coloration of the liquid takes place. The amount of sulphocyanide found in the solution by this means indicates how much it must be diluted to be of the proper strength. Titrate once more with 10 c. c. AgNO3 solution and correct the sulphocyanide solution by the careful addition of water until 20 c. c. exactly correspond to 10 c. c. of the silver solution. The determination of the chlorine in the urine is performed by this method in the following way : Exactly 10 c. c. of the urine are placed in a flask which has a mark corresponding to 100 c. c. ; 5 c. c. nitric acid are added ; dilute with about 50 c. c. water, and then allow exactly 20 c. c. of the silver-nitrate solution to flow in. Close the flask with the thumb and shake well, slide off the thumb and wash it with distilled water into the flask, and fill the flask to the 100-c. c. mark with distilled water. Close again with the thumb, carefully mix by shaking, and filter through a dry filter. Measure off 50 c. c. of the filtrate by means of a pipette, add 3 c. c. ferric-salt solution, and allow the sulphocyanide solution to flow in until the liquid above the precipitate has a permanent red color. The calculation is very simple. For example, if 4.6 c. c. of the sulphocyanide solution were necessary to produce the final reaction, then for 100 c. c. of the filtrate ( = 10 c. c. urine) 9.2 c. c. of this solution are necessary. 9.2 c. c. of the sulphocyanide solution corresponds to 4.6 c. c. of the silver solution, and since 20 — 4.6 = 15.4 c. c. of the silver solution were necessary to completely precipitate the chlorides in 10 c. c. of the urine, then 10 c. c. con- tain 0.154 grm. NaCl. The amount of sodium chloride in the urine is therefore 1.54$ or 15.4 °/w. If we always use 10 c. c. for the determination, and always 20 c. c. AgN03 , and dilute with water to 100 c. c,, we find the amount of NaCl in 1000 parts of the urine by subtracting the number of c. c. of sulphocyanide (R) required with 50 c. c. of the filtrate from 20. The quantity of NaCl p. m. is therefore under these circumstances = 20 — R, and the percentage . ,T ™ 20 — R of NaCl — — — — . The approximate estimation of chlorine in the urine (which must be free from albumin) is made by strongly acidifying with nitric acid and then adding to it, drop by drop, a concentrated silver-nitrate solution (1 : 8). In a normal amount of chlorides the drop sinks to the bottom as a rather compact cheesy lump. In diminished amounts of chlorides the precipitate is less compact and coherent, and in the presence of very little chlorine a fine white precipitate or only a cloudiness or opalescence is obtained. Phosphates. Phosphoric acid occurs in acid urines partly as double-, MH8P04, and partly as simple-acid, M2HP04 , phosphates, 360 PHYSIOLOGICAL CHEMISTRY. both of which are found in acid urines at the same time. OTT found that on an average 60$ of the total phosphoric acid was double- and 40$ was simple-acid phosphate. The total quantity of phosphoric acid is very variable and depends on the kind and the quantity of food. The average amount of P205 is in round num- bers 2.5 grms., with a variation of 1-5 grms., per 24 hours. The phosphoric acid of the urine originates to a small extent from the burning of organic compounds, nuclein, protagon and lecithin, within the organism. The greater part originates from the phos- phates of the food, and the quantity of eliminated phosphoric acid is greater when the food is rich in alkali phosphates in proportion to the quantity of lime and magnesia phosphates. If the food con- tains much lime and magnesia, large amounts of earthy phosphates are eliminated by the excrements ; and even though the food con- tains considerable amounts of phosphoric acid in these cases, the quantity of phosphoric acid in the urine is small. Such a condition is found in the herbivora, whose urine is habitually poor in phos- phates. The extent of the elimination of phosphoric acid by the urine depends not only upon the total quantity of phosphoric acid in the food, but also upon the relative amounts of alkaline earths and the alkali salts in the food. From the transformation of tissues rich in proteid or of phos- phorized nerve-substance in the body we might perhaps expect an equal relation between the nitrogen and the phosphoric acid in the urine. Many investigations have been made upon this subject by ZTJELZER, STRUBING, and EDLEFSSE^; but as all the conditions which affect the elimination of phosphoric acid are not yet suffi- ciently known, it is difficult to draw any definite conclusions from the observations thus far made. Little is known in regard to the elimination of phosphoric acid in disease. In febrile diseases the amount of phosphoric acid is considerably decreased as compared with the urea (ZUELZER). In diseases of the kidneys the activity of these organs in eliminating the phosphates is considerably diminished (FLEISCHER). In men- ingitis, on the contrary, a marked increase in the phosphates is observed in the urine. TEISSIER has described a special form of polyuria, in which abundant quantities of earthy phosphates, 10-20-30 grms. per 24 hours, were eliminated. This polyuria was THE URINE. 381 called PHOSPHATE DIABETES by TEISSIER. The statements in regard to the amount of phosphate in the urine in rachitis and in osteomalacia are somewhat contradictory. A diminished elimina- tion of phosphoric acid has been observed by STOKVIS in arthritis. Quantitative estimation of phosphoric acid in the urine. This estimation is most simply performed by titrating with a solution of uranium acetate. The principle of the titration is as follows : A warm solution of phosphates containing free acetic acid gives a whitish-yellow precipitate of uranium phosphate with a solution of a uranium salt. This precipitate is insoluble in acetic acid, but dissolves in mineral acids, and on this account we always add in titrating a certain quantity of sodium acetate solution. Potassium ferrocyanide is used as the indicator, which does not act on the uranium-phosphate precipitate, but gives a reddish-brown precipi- tate or coloration in the presence of the smallest amount of soluble uranium salt. The solutions necessary for the titration are : 1. A solution of a uranium salt of which each c. c. corresponds to 0.005 grm. P205 and which contains 20.3 grms. uranium oxide per litre. 20 c. c. of this solution corresponds to 0.100 grm. P205. 2. A solu- tion of sodium acetate ; 3. A freshly-prepared solution of potas- sium ferrocyanide. The uranium solution is prepared from uranium nitrate or acetate. Dis- solve about 35 grms. uranium acetate in water, add some acetic acid to facilitate solution, and dilute to one litre. The strength of this solution is determined by titrating with a solution of sodium phosphate of known strength (10.085 grms. crystallized salt in 1 litre, which corresponds to 0.100 grm. P2O5 in 50 c. c.). Proceed in the same way as in the titration of the urine (see below) and correct the solution by diluting with water, and titrate again until 20 c.c. of the uranium solution correspond exactly to 50 c. c. of the above phosphate solution. The sodium-acetate solution should contain 10 grms. sodium acetate and 10 grms. cone, acetic acid in 100 c. c. For each titration 5 c. c. of this solution is used with 50 c. c. of the urine. In performing the titration, mix 50 c. c. of filtered urine in a beaker with 5 c. c. of the sodium acetate, cover the beaker with a watch-glass, and warm over the water-bath. Then allow the ura- nium solution to flow in from a burette, and, when the precipitate does not seem to increase, place a drop of the mixture on a porce- lain plate with a drop of the potassium-ferrocyanide solution. If the amount of uranium solution employed is not sufficient; the color remains pale yellow and more uranium solution must be added; but as soon as the slightest excess of uranium has been used, the color becomes faint reddish brown. When this point has been obtained, warm the solution again and add another drop. If the color remains of the same intensity, the titration is ended ; but if 382 PHYSIOLOGICAL CHEMISTRY. the color varies, add more uranium solution, drop by drop, until a permanent coloration is obtained after warming, and now repeat the test with another 50 c. c. of the urine. The calculation is so simple that it is unnecessary to give an example. In the above manner we determine the total quantity of phos- phoric acid in the urine. If we wish to know the phosphoric acid combined with alkaline earths or with alkalies, we first determine the total phosphoric acid in a portion of the urine and then remove the earthy phosphates in another portion by ammonia. The precip- itate is collected on a filter, washed, transferred in a beaker with water, treated with acetic acid, and dissolved by warming. This solution is now diluted to 50 c. c. with water, and 5 c. c. sodium- acetate solution added, and titrated with uranium solution. The difference between the two determinations gives the quantity of phosphoric acid combined with the alkalies. Sulphates. The sulphuric acid of the urine originates only to a very small extent from the. sulphates of the food. A dispropor- tionally greater part is formed by the burning of the proteids con- taining sulphur within the body, and it is chiefly this formation of sulphuric acid from the proteids which gives rise to the previously- mentioned excess of acids over the bases in the urine. The quan- tity of sulphuric acid eliminated by the urine amounts to about 2.5 grms. H2S04 per twenty-four hours. As the sulphuric acid chiefly originates from the proteids, it follows that the elimination of sulphuric acid and the elimination of nitrogen are nearly parallel, and the relationship N : H2S04 is about 5 : 1. Sulphuric acid occurs in the urine partly preformed (sulphate-sulphuric acid) and partly as ethereal sulphuric acid. The quantity of total sulphuric acid is determined in the fol- lowing way, but at the same time the precautions described in other works must be observed: 100 c. c. of filtered urine are treated with 5 c. c. concentrated hydrochloric acid and boiled for fifteen minutes. While boiling precipitate with 2 c. c. of a satu- rated BaCl2 solution and warm for a little while until the barium sulphate has completely settled. The precipitate must then be washed with water or with alcohol and ether (to remove resinous substances) and then treated according to the usual method. The separate determination of the sulphate-sulphuric acid and the ethereal sulphuric acid may be done, according to BAUMASTN'S method, by first precipitating the sulphate-sulphuric acid from the urine acidified with acetic acid, by BaCla , and then decomposing the ethereal sulphuric acid by boiling after the addition of hydro- THE URINE. 383 chloric acid, and then determining the sulphuric acid set free as barium sulphate. A still better method is the following suggested ,by SALKOWSKI: 200 c. c. of urine are precipitated by an equal volume of a barium solution which consists of 2 vols. barium hydrate and 1 vol. barium-chloride solution, both saturated at the ordinary temper- ature. Filter through a dry filter, measure off 100 c. c. of the filtrate which contains only the ethereal sulphuric acid, treat with 10 c. c. hydrochloric acid of a specific gravity 1.12, boil for fifteen minutes, and then warm on the water-bath until the precipitate has completely settled and the supernatant liquid is entirely clear. Wash with warm water and with alcohol and ether and proceed according to the generally-prescribed method. The difference between the ethereal sulphuric acid found and the total quantity of sulphuric acid as determined in a special portion of urine is considered as the quantity of sulphate-sulphuric acid. Nitrates occur in small quantities in human urine (SCHONBEIN), and they probably originate from the drinking-water and the food. According to WEYL and CITRON, the quantity of nitrates is smallest with a meat diet and greatest with vegetable food. The average amount is about 42.5 milligrammes per litre. Potassium and Sodium. The quantity of these bodies eliminated by the urine by a healthy full-grown person on a mixed diet is, according to SALKOWSKI, 3-4 grms. K20 and 5-7.5 grms. NaaO. The proportion of K to Na is ordinarily as 3:5. The amount depends above all upon the food. In starvation the urine may become richer in potassium than in sodium, which results from the lack of common salt and the destruction of tissue rich in po- tassium. The quantity of potassium may be relatively increased during fever, while after the crisis the reverse is the case. The quantitative estimation of these bodies is performed by the gravimetric methods as described in analytical works. Ammonia. Some ammonia is habitually found in human urine and in that of carnivora. This ammonia may represent, as above stated (page 338), on the formation of urea from ammonia, the small amount of ammonia which, because of the excess of acids formed by the combustion, as compared to the fixed alkalies, is united with such acids and in this way excluded from the synthesis to urea. These views are confirmed by the observations of Co- RANDA, who found that the elimination of ammonia was smaller on a vegetable diet and larger on a rich meat diet than when on a 384 PHYSIOLOGICAL CHEMISTRY. mixed diet. On a mixed diet the average amount of ammonia eliminated by the urine is about 0.7 grm. NH3 per twenty-four hours (NEUBAUER). The quantity of ammonia in human urine and that of carnivora is increased by the introduction of mineral salts and also in diseases in which an increased formation of acid takes place due to an in- creased metabolism of proteids. This is the case in fevers and diabetes. In the last-mentioned disease an organic acid, /?-oxybu- tyric acid, is produced (MINKOWSKI, KULZ, STADELMAKN) which passes into the urine combined with ammonia. In diseases of the liver, as in acute yellow atrophy and interstitial hepatitis (HALLER- VORDEH, STADELMANN), the formation of urea may decrease and the elimination of ammonia increase. In these cases the propor- tion of NH3 : Ur, which, according to STADELMAKX, is normally •2.8 : 100, may be changed. The same may also be observed in acute phosphorus-poisoning. In such a case K. MORNER found the relation 5.2 : 100. The detection and quantitative estimation of ammonia is per- formed according to the method suggested by SCHLOSING. The principle of this method is that the ammonia from a measured amount of urine is set free by lime-water in a closed vessel and N absorbed by a measured amount of JA sulphuric acid. After the absorption of the ammonia the quantity is determined by titrating N the remaining free sulphuric acid with a JQ caustic alkali. This method gives low results, and in exact work we must proceed as suggested by BOHLAND (PFLUGER'S Archiv, vol 43, page 32). Other methods have been suggested by SCHMIEDEBERG and by LATSCHEN- BERGER. Calcium and magnesium occur in the urine for the most part as phosphates. The quantity of earthy phosphates eliminated daily is somewhat more than 1 gr., and of this amount f is magnesium and | calcium phosphate. In acid urines the simple- as well as the double-acid earthy phosphates are found, and the solubility of the first, among which the calcium-salt, CaHP04, is especially insol- uble, is particularly augmented by the psesence of double-acid al- kali phosphate and sodium chloride in the urine (OTT). The quan- THE URINE. 385 tity of alkaline earths in the urine depends on the composition of the food. Nothing is known with positiveness in regard to the con- stant and regular change in the elimination of these substances in disease. The quantity of calcium and magnesium is determined accord- ing to the ordinary well-known methods. Iron occurs in the urine only in small amounts, and, as it seems, not as a salt, but as an organic combination — part perhaps as pigment or chromogen (KuNKEL, GIACOSA) and part in other forms. According to MAGNIER, the quantity of iron in 1 litre of urine is 3-11 inilligrms. According to GOTTLIEB, the elimination of iron by the healthy human urine amounts to 2.59 milligrms. per day. Iron-salts introduced into the intestine do not pass into the urine at all, or only in very small amounts. The quantity of silicic acid, according to the ordinary statements, amounts to about 0.03 p. m. Traces of hydrogen per- oxide also occur in the urine. The gases of the urine are carbon dioxide, nitrogen, and traces of oxygen. The quantity of nitrogen is not quite 1 vol. per cent. The carbon dioxide varies considerably. In acid urines it is hardly one half as great as in neutral or alkaline urines. IV. The Amount and Quantitative Composition of Urine. A direct participation of the kidney substance in the formation of the urinary constituents is proved at least for hippuric acid. It is hardly to be doubted that the kidneys as well as the tissues gen- erally have a certain part to play in the formation of other urinary constituents, but their chief task consists in separating and remov- ing urinary constituents dissolved in the blood which have been taken up by it from other organs and tissues. It has been shown by the experiments of numerous investiga- tors, HEIDEtfHAItf, V. WlTTICH, NlJSSBAUM, 1STEISSEE, USTIMO- WITSCH, J. MTOK, and others, that the elimination of water and the remaining urinary constituents is not alone produced by simple diffusion and filtration. It is generally conceded that the processes of urinary secretion depend essentially upon a specific activity of the cells of the epithelium of the urinary passages, besides which also processes of filtration and diffusion take part. The process of the secretion of urine in man and the higher animals is generally considered to proceed chiefly as follows: The water together with 386 PHYSIOLOGICAL CHEMISTRY. a small amount of the salts passes through, the glomeruli Mal- pighii, while the chief part of the solids is secreted by the epithe- lium of the urinary passages. A secretion of solids without a simul- taneous secretion of water is not possible, and therefore a part of the water must be secreted by the epithelium-cells of the urinary passages. The passage of the chief part of the water through the glomeruli is rather generally considered as a filtration due to blood- pressure. According to HEIDEJ^HAIN", the thin cell-layers of the glomeruli have a secretory action. The amount and the composition of urine is liable to great variation. Those circumstances which under physiological condi- tions exercise a great influence are the following: the blood-pres- sure, and the rapidity of the blood-current in the glomeruli; the quantity of urinary constituents, especially water in the blood; and lastly, the condition of the secretory glandular elements. Above all, the amount and concentration of the urine depend on the elimination of water. That this last may vary with the amount of water in the blood, with changed blood-pressure, and with cir- culatory conditions is evident; but under ordinary circumstances the amount of water eliminated by the kidneys depends essentially upon the quantity of water which is brought to them by the blood, or which leaves the body by other exits. The elimination of urine is increased by abundant drinking or if the amount of water re- moved in other ways is lessened; but it is decreased by a dimin- ished introduction of water, or by a greater loss of water in other ways. Ordinarily in man just as much water is eliminated by the kidneys as by the skin, lungs, and intestines together. At lower temperatures and in moist air, since under these conditions the elimination of water by the skin is diminished, the elimination of urine may be considerably increased. Diminished introduction of water or diminished secretion of water — as in violent diarrhoea, vio- lent vomiting, or abundant perspiration — greatly diminishes the elimination of urine. For example, the urine may sink as low as 500-400 c. c. per day in intense summer-heat, while after copious draughts of water the elimination of 3000 c. c. of urine has been observed during the same time. The average quantity of urine secreted in the course of 24 hours for healthy grown men is 1500 c. c., and for women 1200 c. c. The minimum secretion occurs THE URINE. 387 during the night between 2-4 o'clock; the maximum, in the first hours after awakening and from 1-2 hours after a meal. The quantity of solids secreted in the course of 24 hours is rather constant even though the quantity of urine may vary, and it is more constant when the manner of living is regular. There- fore the percentage of solids in the urine is naturally in an inverse proportion to the quantity of urine. The average quantity of solids per 24 hours is calculated as 60 grins. The quantity may be calculated with approximate accuracy by means of the specific gravity, if the second and third decimals of the specific gravity be multiplied by HASER'S coefficient 2.33. The product gives the amount of solids in 1000 c.c. of urine, and if the quantity of urine eliminated in the 24 hours be measured, the quantity of solids in the 24 hours may be easily calculated. For example, 1050 c.c. of urine of a sp. gr. 1.021 was eliminated in the 24 hours; therefore the quantity of solids eliminated is 21 X 2.33 = 48.9, and - ' = 51.35 grms. The urine in this case contained 48.9 p. m. solids and 51.35 grms. in the daily secretion. Those bodies which, under physiological conditions, affect the density of the urine are common salt and urea. The specific gravity of the first is 2.15 and the last only 1.32, so it is easy to understand, when the relative proportion of these two bodies essentially de- viates from the normal, why the above calculation from the specific gravity is not exact. The same is the case when a urine poor in a normal constituent contains large amounts of foreign bodies, such as albumin or sugar. As above stated, the percentage of solids in the urine generally decreases with a greater elimination, and an abundant secretion (polyuria) has therefore, as a rule, a lower specific gravity. An important exception to this rule is observed in urine containing sugar (diabetes mellitus), in which there is a very abundant secre- tion of a very high specific gravity due to the sugar. In cases where very little urine is secreted (oligurid), as when the perspira- tion is profuse, in diarrhoea, and in fevers, the specific gravity is as a rule high, the percentage of solids high, and the color dark. Sometimes, as, for example, in certain cases of albuminuria, the 388 PHYSIOLOGICAL CHEMISTRY. reverse of the above may be observed even though the urine has a low specific gravity, a pale color, and is poor in solids. It is difficult to give a tabular view of the composition of urine, on account of its variation. For certain purposes the following table may be of some value, but it must not be overlooked that the results are not given for 1000 parts of urine, but only approximate figures for the amounts of the most important constituents which are eliminated in the course of 24 hours in a quantity of 1500 c.c. Daily amount of solids = 60 grms. Organic constituents = 35 grms. Urea 30 Uricacid 0.7 Creatinin 1.0 Hippuric acid 0.7 Remaining organic bodies 2.6 Inorganic constituents. . = 25 grms. Sodium chloride (NaCl). 15.0 Sulphuric acid (H2SO4). 2.5 Phosphoric acid (PaO»).. 2.5 Potash (K2O) 3.3 Ammonia (NH8) 0.7 Magnesia (MgO) 0.5 Lime(CaO) 0.3 Remaining inorg. bodies 0.2 Urine contains on an average 40 p. m. solids. The amount of urea is about 20 p. m. and common salt about 10 p. m. V. Casual Urinary Constituents. The casual appearance in the urine of medicines or of urinary constituents resulting from the introduction of foreign substances into the organism is of practical importance, 'because such constit- uents may interfere in certain urinary investigations and also because thev afford a good means of determining whether certain substances have been introduced into the organism or not. From this point of view a few of these bodies will be spoken of in a following section (on the pathological urinary constituents). The presence of these foreign bodies in the urine is of special interest in those cases in which they serve to elucidate the chemical trans- formations certain substances undergo within the body. As inor- ganic substances generally leave the body unchanged, they are of very little interest from this standpoint, but the changes which certain organic substances undergo may be studied by this means so far as these transformations are shown by the urine. The bodies belonging to the fatty series, though not without exceptions, fall mostly into a combustion leading towards the end- THE URINE. 389 products of the exchange of material; still, often a smaller or greater part of the body in question withdraws itself from oxidation •and appears unchanged in the urine. A part of the organic acids, which are otherwise burnt into1 water and carbonates and render the urine neutral or alkaline act in this way. The volatile fatty acids poor in carbon are less easily burnt than those rich in carbon, and they therefore pass in large amounts unchanged into the urine. This is especially true of formic and acetic acids (SCHOTTEN, GREHAKT and QUINQUAND). Oxalic acid passes completely or almost completely unchanged into the urine (GAGLIO). The acid amides appear not to be changed in the body (SCHULTZEN and NENCKI). A small part of the amido-acids seem indeed to be eliminated unchanged, but otherwise they are, as stated above (page 338) for leucin, glycocoll and aspartic acid, decomposed within the body, and they may therefore cause an increased elimi- nation of urea. Sarcosin (methylglycocoll), NH(CH3).CH2.COOH, also perhaps passes in small part into the corresponding uramido- acid, methylhydantoinic acid, NPI2.CO.N(CH3).CH2.COOH. Also taurin, amido-ethylsulphonic acid, which acts somewhat differently in different animals (SALKOWSKI), passes in human beings, at least in part, into the corresponding uramido-acid, taurocarbaminic acid, NH2.CO.NH.C2H4.S02.OH. A part of the taurin appears as such in the urine. In rabbits, when taurin is introduced into the stomach, nearly all its sulphur appears in the urine as sulphuric and sulphurous acids. After subcutaneous injection the taurin appears again in great part unchanged in the urine. Creatin passes, at least in part, into creatinin. A part may perhaps appear as urea (see page 257). Hypoxanthin passes into uric acid. A coupling with glycocoll may also occur. Furfur ol or the anhydride of pyromucic acid, C5H402, passes to a great extent coupled with glycocoll as pyromucuric acid, C7H7N40, into the urine of rabbits and dogs and to a less extent as furfuracrylic acid coupled with glycocoll as furfuracryluric acid, CsH^O (JAFFE and COHN). In birds (hens) this condition is different. In these animals furfurol gives pyromucic acid and a coupled acid, pyromucinornithuric acid, C15H16N206 , which decomposes on warm- ing with concentrated hydrochloric acid into pyromucic acid and ornithin, C6H12N202 (JAFFE and COHN). 390 PHYSIOLOGICAL CHEMISTRY. Coupling with glycuronic acid occurs in certain substituted alcohols, aldehydes and ketones (?), which probably first pass over into alcohol (SUNDVIK). Chloral hydrate, C2C]3OH4-H20, passes, after it has been converted into trichlorethyl-alcohol by a reduc- tion, into a laevo-gyrate reducible acid, itrochloralic acid or trichlorethyl-glycuronic acid, CgClaHg.CeHjO; (MuscuLUS and v. MEEING). Trichlorbutyl-alcohol and butyl-chloral hydrate also pass into trichlorbutyl-glycuronic acid. Tertiary amyl- and butyl- alcohol also undergo (in rabbits but not in man) a coupling with glycuronic acid. In animals which have starved until the glycogen has disappeared from the muscles and liver and which are given chloral hydrate or dimethyl carbinol, coupled glycuronic acids appear in the urine (THIEEFELDEE). On account of these facts the albuminous bodies are considered the origin of the glycuronic acid.. It may perhaps originate from such bodies as the proteids which are found widely diffused in the body and from which carbohydrates or near-related acids may be split. The aromatic combinations pass as a rule, so far as we know, into the urine after a previous partial oxidation or after a synthesis with other bodies. The question whether the benzol ring is destroyed in the body is still undecided, but at least in certain cases such a destruction is very probable. The fact that benzol may be oxidized outside of the body into carbon dioxide, oxalic acid, and volatile fatty acids has been known for a long time, and we may refer the reader to the investigations of DEECHSEL, mentioned in the first chapter, in which this experimenter obtained, by the electrolysis of phenol, normal caproic acid and afterward substances in which the amount of carbon decreased constantly until he obtained the end-products of the exchange of material. As in these experiments a splitting of the benzol ring must take place before the formation of the body of the fatty series, also when aromatic bodies are burnt in the animal body, we must admit that first a rupture of the benzol ring takes place with the formation of fatty bodies. If this does not take place, then the benzol nucleus is eliminated with the urine as an aromatic combination of one kind or another. As the difficultly - burnt benzol nucleus can protect from destruction a substance belonging to the fatty, series and coupled with it, which is the case THE URINE. 391 with the glycocoll of hippuric acid, it seems also that the aromatic nucleus itself may be protected from destruction in the organism by syntheses with other bodies. The aromatic ethereal sulphuric acids are examples of this kind. The difficulty in deciding whether the benzol ring itself is destroyed in the body lies in the fact that we do not know all the different aromatic transformation products which may be pro- duced by the introduction of any aromatic substance in the organism and which we must seek for in the urine. On this account it is also impossible to learn by exact quantitative estima- tions whether or not an aromatic substance introduced or absorbed appears again in its entirety in the urine. Certain observations render it probable that the benzol ring, as above mentioned, is at least in certain cases destroyed in the body. SCHOTTEN and BAU- MANN" have found that certain amido-acids, such as tyrosin, plienylamido-proinonic acid and amido-cinnamic acid, when intro- duced into the body cause no increase in the quantity of known aromatic substances in the urine; this makes a destruction of these amido-acids in the animal body seem probable. JUVALTA also made an experiment on dogs with phthalic acid and found that 57.5-68.76$ of the acid introduced into the body disappeared, or more correctly was not found again. According to JUVALTA, this acid does not undergo any synthesis, nor does it yield any aromatic transformation products ; and if this supposition be correct, we have here a proof of the destruction of the benzol nucleus of a part of the phthalic acid introduced into the organism of the dor, An oxidation in the side chain of aromatic compounds is often found and may also occur in the nucleus itself. As an example, benzol is first oxidized to oxybenzol (ScHULTZEN" and NAUNYN), and this is then in part converted into dyoxybenzols (BAUMAiO" and PREUSSE). Naphthalin appears to be converted into oxynaphthalin and probably a part also into dioxynaphthalin (LESLIE: and M. NENCKI). Anilin, C6H5.NH2, passes into paramidophenol, which passes into the urine as ethereal sulphuric acid, H2N.C6H4.O.S02.OH (F. MTJLLEE). If the aromatic substance has a side chain belonging to the fatty series, this last is generally oxidized. For example, toluol, C6H6.CH3 (SCHULTZEN and NAUNYN), ethyl-benzol, C6H5.C2H6, and propyl- 392 PHYSIOLOGICAL CHEMISTRY. benzol, C6H5.C3H7 (NENCKI and GIACOSA), also many other bodies are oxidized into benzoic acid. If the side chain has several mem- bers, the behavior is somewhat different. Phenol-acetic acid, C6H5. CH2.COOH, in which only one carbon atom exists between the benzol nucleus and the carboxyl, is not oxidized, but is eliminated after coupling with glycocoll as plienaceturic acid (SALKOWSKI). Phenyl-propionic acid, C6H5.CH2.CH2.COOH, with two carbon atoms between the benzol nucleus and the carboxyl is, on the contrary, oxidized into benzoic acid. Aromatic amido-acids with three car- bon atoms in the side chain, and where the NH2 group is bound to the middle one, as in ty rosin, ar-oxyphenylamido-propionic acid, C6Hi(OH).CH2.OH(NH2).COOH, and a-plienylamido-propionic acid, C6H5.CH2.CH(NH2).COOH, seem to be in great part burnt within the body (ScHOTTEN" and BAUMAKN"). Phenylamido-acetic acid, which has only two carbon atoms in the side chain, C6H5.CH(NH2). COOH, acts otherwise, passing into mandelic acid, phenyl-glycolic acid, C6H5.CH(OH).COOH (SCHOTTEN). If several side chains are present in the benzol nucleus, then only one is always oxidized into carboxyl. Thus xylol, C6H4(CH3)2 , is oxidized into toluic acid, C6H4(CH3)COOH (SCHULTZEN* and NAUKYH), mesitylen, C6H3(CH3)3 , into mesitylenic acid, C6H3(CH3)2. COOH (L. NENCKI), and cymol into cumic acid (M. NENCKI and ZIEGLER). Syntheses of aromatic substances with other atomic groups occur frequently. To these syntheses belongs, in the first rank, the coupling of benzoic acid with glycocoll to form Jiippuric acid, first discovered by WOHLER. All the numerous aromatic sub- stances which are converted into benzoic acid in the body are voided as hippuric acid. This statement is not true for all classes of animals. According to the observations of JAFFE, benzoic acid does not pass into hippuric acid in birds, but into another more nitrogenized acid, ornithuric acid, C19H20N204. This acid yields as splitting products, besides benzoic acid, a basic body, ornithin (see page 389). Oxybenzoic acids (salicylic acid passes partly into sali- cyluric acid) and the substituted benzoic acids form pairs with glycocoll corresponding to hippuric acid, but also with the above- mentioned acids, toluic, mesitylenic, cumic, and phenyl-acetic acid. THE UEINE. 393 These acids are voided as toluric, mesitylenuric, cuminuric, and phenaceturic acid. Another synthesis of aromatic substances is that of the ethereal 'sulphuric acids. Phenol and chiefly the hydroxylated aromatic hydrocarbons are voided as ethereal sulphuric acids, according to BAUMANIT, HEETEE, and others. A pairing of aromatic substances with glycuronic acid, which last is protected from burning, occurs rather often. Camphor, C10H160, when given to a dog, is first converted by oxidation into camphorol, C10H15(OH)0, and by coupling with glycuronic acid, campho- glycuronic acid is produced (SCHMIEDEBEEG). Borneol and menthol give directly, with the elimination of water, the corre- sponding glycuronic acids (PELLACA:NT). Phenol may also be partly voided directly as a coupled glycuronic acid (SCHMIEDEBEEG). Naphthol appears in great part to pass into the urine as coupled glycuronic acids (LESLIE and M. NENCKI). Orthonitrotoluol in dogs passes first into orthonitrobenzyl-alcohol and then into a coupled glycuronic acid (JAFFE). Indol (SCHMIEDEBEEG) and skatol (MESTEE) seem, as above mentioned, to be partly voided by the urine as coupled glycuronic acids. The same is true also for many other aromatic substances. A synthesis in which a compound containing sulphur, mercap- turic acid, occurs is produced by the introduction of chlorine or bromine derivatives of benzol into the organism of dogs (BAUMANN" and PEEUSSE, JAFFE). Ghlorbenzol combines with cy stein, a body which seems to be a decomposition product of the albumins, and which is nearly related to cystin (see below), forming chlor- phenylmercapturic acid, CnHjaClSNOg. On boiling with a mineral acid this compound is decomposed into acetic acid and chlorphenyl- cystein, C6H4C1. C3H6lSrS02. Pyridin, C5H5N, which does not combine either with glycuronic acid or with sulphuric acid after previous oxidation, shows a special behavior. It takes up a methyl group and forms an ammonium combination, methylpyridyl-ammonium hydroxyl (His). Several alkaloids, such as quinin, morphin, and strychnin, may pass into the urine. After taking turpentine, balsam of copaiva, and resins these may pass into the urine as resin acids (MALT). Different kinds of coloring matters, such as alizarin, crysophanic acid, after 394 PHYSIOLOGICAL CHEMISTRY. the use of rhubarb or senna, and the coloring matter of the blue- berry, may pass into the urine. After taking rhubarb, senna or santonin the urine takes a yellow or greenish-yellow color, which is transformed into a beautiful red color by the addition of alkali. Phenol produces, as above mentioned, a dark-brown or dark-green color which depends mainly on the decomposition products of hydrochinon or humin substances (v. UDRAJSTSZKY). After the use of naphthalin the urine has a dark color, and several other medi- cines produce a special coloration. Thus cairin gives often a yel- lowish-green hue, or the urine darkens when exposed to the air; thallin gives a greenish-brown color which is marked green in thin layers, and antipyrin gives a yellow to blood-red. After the admin- istration of balsam of copaiva the urine becomes, when strongly acidified with hydrochloric acid, gradually rose and purple-red (QuiNCKE). After the use of naphthalin or naphthol the urine gives with concentrated sulphuric acid (1 c. c. concentrated acid and a few drops of urine) a beautiful emerald-green color (PEN- ZOLDT), which is probably due to naphthol-glycuronic acid. Odor- iferous bodies also pass into the urine. After eating asparagus the urine acquires a sickly disagreeable odor. After taking turpentine the urine may have a peculiar odor similar to that of violets. VI. Pathological Constituents of Urine. Albumin. The appearance of slight traces of albumin in the urine of apparently healthy persons has been observed in many cases by several investigators (LEUBE, HOFMEISTER, POSTER, and others), but still we must not conceal the fact that other investiga- tors consider these traces of albumin as the first symptoms, though very mild, of a diseased condition of the urinary apparatus, or as a symptom of a transitory disturbance in the circulation. Frequently traces are found in the urine of a substance similar to nucleoalbu- min which can easily be mistaken for mucin and which is probably identical with nucleoalbumin. This substance has been isolated from the pupillary part of the kidneys and from the mucous mem- brane of the bladder by LONNBERG. In diseased conditions albu- min occurs in the urine in a variety of cases. The albuminous bodies which most often occur are serum -globulin and serum- THE URINE. 395 albumin. Albumoses and peptones also sometimes occur. The amount of albumin in the urine is in most cases less than 5 p. m., rarely 10 p. m., and only very rarely does it amount to 50 p. m. or over. Among the many reactions proposed for the detection of albu- min in urine, the following are to be recommended : The Heat Test. Filter the urine and test its reaction. An acid urine may, as a rule, be boiled without further treatment, and only in especially acid urines is it necessary to first treat with a little alkali. An alkaline urine is made neutral or faintly acid before heating. If the urine is poor in salts, add -fa vol. of a saturated common-salt solution before boiling; then heat to boiling point, and if no precipitation, cloudiness, or opalescence appears, the urine in question contains no coagulable albumin, but it may con- tain albumoses or peptones. If a precipitate is produced on boil- ing, this may consist of albumin, or of earthy phosphates, or of both. The simple-acid calcium phosphate decomposes on boiling, and normal phosphate may separate (STOKVIS, SALKOWSKI, OTT). The proper amount of acid is now added to the urine, so as to pre- vent any mistake caused by the presence of earthy phosphates, and to give a better and more flocculent precipitate of the albumin. If acetic acid is used for this, then add 1-2-3 drops of a 25$ acid to each 10 c. c. of the urine, and boil after the addition of each drop. On using nitric acid, add 1-2 drops of the 25$ acid to each c. c. of the boiling-hot urine. On using acetic acid, when the amount of albumin is very small, and especially when the urine was originally alkaline, the albumin may sometimes remain in solution on the addition of the above quantity of acetic acid. If, on the contrary, less acid is added, the precipitate of calcium phosphate, which forms in amphoteric or faintly-acid urines, is liable not to dissolve completely, and this may cause it to be mistaken for an albumin precipitate. If nitric acid is used for the heat test, the fact must not be overlooked that after the addition of only a little acid a combination between it and the albumin is formed which is soluble while boiling and which is only precipitated by an excess of the acid. On this account the large amount of nitric acid as suggested above must be added, but in this case a small part of the albumin is liable to be dissolved by the excess of the nitric acid. When the acid is added after boil- ing, which is absolutely necessary, the liability of a mistake is not so great. It is on these grounds that the heat test, although it gives very good results in the hands of experts, is not recommended to physicians as a positive test for albumin. A confounding with mucin, when this body occurs in the urine, 396 PHYSIOLOGICAL CHEMISTRY. is easily prevented in the heat test with acetic acid, by acidifying another portion with acetic acid at the ordinary temperature. Mucin and nucleoalbumin substances similar to mucin are hereby precipi- tated. If in the performance of the heat and nitric acid test a precipitate first appears on cooling or is strikingly increased, then this shows the presence of albumoses in the urine, either alone or mixed with coagulable albumin. In this case a further investiga- tion is necessary (see below). In a urine rich in urates a precipi- tate consisting of uric acid separates on cooling. This precipitate is colored, sandy, and hardly to be mistaken for an albumose or albumin precipitate. HELLER'S test is performed as follows (see page 19): The urine is very carefully floated on the surface of nitric acid in a test-glass. The presence of albumin is shown by a white ring between the two liquids. With this test a red or reddish-violet ring is always obtained with normal urine; it depends on the indigo coloring mat- ters and can hardly be mistaken for the white or whitish albumin ring, and this last must not be mistaken for the ring produced by bile-pigments. In a urine rich in urates another complication may occur, due to the formation of a ring produced by the precipitated nric acid. The uric-acid ring does not lie, like the albumin ring, between the two liquids, but somewhat higher. For this reason we may often have two simultaneous rings with urines rich in urates and yet not containing very much albumin. The disturbance caused by uric acid is easily prevented by diluting the urine with 1-2 vol. water before performing the test. The uric acid now remains in solution, and the delicacy of HELLER'S test is so great that after dilution only in the presence of insignificant traces of albumin does this test give negative results. In a urine very rich in urea a ring-like separation of urea nitrate may also appear. This ring consists of shining crystals, and it does not appear in the pre- viously-diluted urine. A confusfon with resinous acids, which also give a whitish ring with this test, is easily prevented, since these acids are soluble on the addition of alcohol. A liquid which con- tains pure mucin does not give a precipitate with this test, but it gives a more or less strongly opalescent ring, which disappears on stirring. The liquid does not contain any precipitate after stirring, but is clear or somewhat opalescent. If we bear in mind the above-mentioned possible mistakes and the means by which they may be prevented, there is hardly another test for albumin in the urine which is at the same time so easily performed, so delicate, and so positive as HELLER'S. With this test even 0.02 p. m. albumin may be detected without difficulty. In performing this test the (primary) albumoses are also precipitated. The reaction with metaphosphoric acid (see page 19) is very convenient and easily performed. It is not quite so delicate and THE URINE. 397 positive as HELLER'S test. The albumoses are also precipitated by this reageut. Reaction with Acetic Acid and Potassium Ferrocyanide. Treat the urine first with acetic acid of about 2$ and then add drop by drop a potassium ferrocyanide solution (1 : 20), carefully avoiding an excess. This test is very good, and in the hands of experts it is even more delicate than HELLER'S. In the presence of very small amounts of albumin it requires more practice and dexterity than HELLER'S, as the relative quantities of reagent to the albumin act on the result of the test. The amount of salts in the urine also seems to have an influence. This reagent also precipitates albumoses. The different color reactions cannot be directly used, especially in deep-colored urines which only contain little albumin. The common salt of the urine has a disturbing action on MILLON'S reagent. To prove more positively the presence of albumin, the precipitate obtained in the boiling test may be filtered, washed, and then tested with MILLON'S reagent. The precipitate may also be dissolved in dilute alkali and the biuret test applied to the solu- tion. The presence of albumoses or peptones in the urine is directly tested for by this last-mentioned test. In testing the urine for albumin one must never be satisfied with one test alone, but one must at least apply the heat test and HELLER'S test or the potassium-ferrocyanide test. In using the heat test alone the albumoses may be easily overlooked, but these are detected, on the contrary, by HELLER'S test. If we are satisfied with this last test or the potassium-ferrocyanide test alone, we have no sufficient inti- mation of the kind of albumin present, whether it consists of albu- moses or coagulable albumin. For practical purposes several dry reagents for albumin have been recom- mended. Besides the metaphosphoric acid may be mentioned : PAVY'S re- agent, which consists of small disks or plates of citric acid and sodium ferrocy- anide ; STUTZ'S or FURBRINGER'S gelatine capsules, which contain mercuric chloride, sodium chloride, and citric acid; and GEISSLEB'S albumin-test papers, which consist of strips of filter-paper which have been dipped in a solution of citric acid and also mercuric chloride and potassium-iodide solution and then dried. If the presence of albumin has been positively proved in the urine by the above tests, it then remains necessary to determine the variety. The detection of globulin and albumin. In detecting serum- globulin the urine is exactly neutralized, filtered, and treated with magnesium sulphate in substance until it is completely saturated at the ordinary temperature, or with an equal volume of a saturated neutral solution of ammonium sulphate. In both cases a white, flocculent precipitate is formed in the presence of globulin. In 398 PHYSIOLOGICAL CHEMISTRY. using ammonium sulphate with a urine rich in urates a precipitate consisting of ammonium urate may separate. This precipitate does not appear immediately, but only after a certain time, and it must not be mistaken for the globulin precipitate. In detecing serum albumin heat the filtrate from the globulin precipitate to boiling- point or add about \ acetic acid to it at the ordinary temperature. In detecting albumoses, whose occurrence in the urine was first shown by BENCE JOI^ES and by KUHKE and later observed by many investigators in different diseased conditions, first remove all coagulable albumins, if any are present, by boiling with the addi- tion of acetic acid. The filtrate is then tested by the biuret test, and when this gives positive results apply the three above-men- tioned albumose reagents (page 26), nitric acid, acetic acid, and potassium ferrocyanide, and saturate with common salt with the addition of acid. The album oses may also be precipitated by sat- urating with ammonium sulphate in substance. Peptones, as shown by the experiments of several investigators, among whom should be especially mentioned HOFMEISTEE, v. JAKSCH, MAIXNEE, and FISCHEL, may appear in the urine as soon as it occurs dissolved in the blood. This is especially the case in an abundant destruction of the pus-cells with absorption of the peptones originating therefrom, as also in pneumonia, in purulent pleuritis, etc. (PYOGEKE PEPTONUEIA, HOFMEISTEE, MAIX^EE, v. JAKSCH). Peptone also passes into the urine when the normal absorption and assimilation of the peptones is disturbed so that the peptones pass directly through the destroyed part of the intes- tine into the blood, as in ulcerous processes in the intestine (EKTEE- OGENIC PEPTOKUEIA, MlXNEE), also in PUEEPEEAL PEPTOITUEIA (FISCHEL), in acute phosphorus-poisoning and certain diseases of the liver (HEPATOGENIC PEPTONUEIA), in effusion of blood in difficult cases of scurvy (H^EMATOGENIC PEPTOKUEIA, v. JAKSCH), etc. etc. These statements refer only to the peptone in the old sense, and the question as to whether we are dealing with "secondary albu- moses " or with " pure peptones" or a mixture of both is still un- decided. Until we are agreed as to the importance of the peptones and albumoses it is hardly possible to give positive statements in regard to the occurrence of peptones in the urine, or to give ac- curate methods for their detection and quantitative estimation. The urine to he tested for peptone in the old sense must be free from mucin and from albumin so that it does not give either the potassium-ferro- THE URINE. 399 cyanide reaction or HELLER'S test. Such a urine may be tested directly ; if, on the contrary, it contains albumin, this must first be removed. This is ordinarily done according to HOFMEISTEK'S method. At least half a litre of , the urine is treated with an excess of a lead-acetate solution, or with only a quantity sufficient to produce a dense flocculent precipitate in order to separate some so-called inucin present as well as a part of the albumin and coloring matters. If the operation has been well conducted, the filtrate, which gives a precipitate with more lead-acetate solution, is tested for albumin. In the absence of this it is directly tested for peptone ; but if albumin be present, it must first be removed by boiling with ferric-acetate solution. For this pur- pose treat the filtrate with a concentrated sodium-acetate solution (about 10 c. c. for ^ litre urine) and then with ferric-chloride solution until it has a blood-red color. The acid-reacting liquid is then rendered neutral or faintly acid by means of the addition of alkali, boiled strongly, and filtered after cooling. The filtrate should be free from albumin ; but if that is not the case, it must be repeatedly treated with sodium acetate and ferric chloride. If the urine to be tested for peptones is rather rich in albumin at first, the albumin must be removed as far as possible by boiling with the addition of acetic acid before it is treated with lead-acetate solution as above described. A small portion of the filtrate entirely free from albumin is taken, made strongly acid with acetic acid and then treated with an acetic-acid solution of phospho-tungstic acid. If the test remains clear for some time, the urine con- tains no peptones ; but if a milky cloudiness occurs, peptones may be present and the filtrate must be further treated. For this purpose add ^-^ vol. concentrated hydrochloric acid and then add a phospho-tungstic acid solution treated with acid as long as a precipitate is produced. This is quickly filtered and treated with water which contains 3-5$ concentrated sulphuric acid, washed, until the filtrate is colorless. The still moist precipitate is thoroughly rubbed with an excess of solid barium hydrate, some water added, gently warmed for a time, and filtered. The peptones (and secondary albumoses) are detected in the filtrate by applying the previously-mentioned reactions. Special stress must be laid on the biuret reaction, which is used also as a colorimetric quantitative test for peptones. In detecting pure peptones the solution must be saturated with ammonium sulphate while boiling, and filtered at the same temperature. After cooling remove the liquid from the deposited crystals, dilute strongly, precipitate the peptone by the careful addition of tannic acid, treat the precipitate with an excess of barium hydrate, and use the filtrate in which the excess of dissolved barium hydrate has been removed by COa for the biuret test. Still by this procedure the peptones may be contaminated by some albumoses. We have no very exact method for the quantitative estimation of albumose or peptones in the urine. Quantitative Estimation of Albumin in Urine. Of all the methods proposed thus far, the COAGULATION METHOD (boiling with the addition of acetic acid) when performed with sufficient care gives the best results. The average errors need never amount to more than 0.01$, and it is generally smaller. In using this method it is best to first find how much acetic acid must be added to a small portion of urine, which has been previously heated on the water-bath, to completely separate the albumin, so that the filtrate does not respond to HELLER'S test. Then coagulate 20-50-100 c. c. of the urine. Pour the urine into a beaker and heat on the water-bath, add the required quantity of acetic acid slowly, stirring 400 PHYSIOLOGICAL CHEMISTRY. constantly and heating at the same time. Filter while warm, wash first with water, then with alcohol and ether, dry and weigh, ash. and weigh again. In exact determinations the filtrate must not give HELLER'S test. The separate estimation of GLOBULINS and ALBUMINS is done by carefully neutralizing the urine and precipitating nvith MgS04 added to saturation (AUTHOR), or simply by adding an equal volume of a saturated neutral solution of ammonium sulphate (HOFMEISTER and POHL). The precipitate consisting of globulin is thoroughly washed with a saturated magnesium sulphate or half- saturated ammonium-sulphate solution, dried continuously at 110° 0., boiled with water, extracted with alcohol and ether, then dried, weighed, ashed, and weighed again. The quantity of albumin is calculated as the difference between the quantity of globulins and the total proteids. Approximate Estimation of Albumin in Urine. Of the methods suggested for this purpose none has been more extensively employed than ESBACH'S. ESBACH'S method. The acidified urine (acidified with acetic acid) is poured into a specially-graduated tube to a certain mark and then the reagent (a 2$ citric-acid and \% picric-acid solution •in water) is added to a second mark, the tube is closed with a rubber stopper and carefully shaken, avoiding the production of froth. The tube is allowed to stand twenty-four hours, and then the height of the precipitate in the graduated tube is read off. The reading gives directly the quantity of albumin in 1000 parts of the urine. Urines rich in albumin must first be diluted with water. The results obtained by this method are, however, dependent upon jthe temperature; and a difference in temperature of 5° to 6.5° C. may in urines containing a medium quantity of albumin cause an error of 0.2-0.3$ deficiency or excess (CHRISTENSEN and MYGGE). This method is only to be used in a room in which the temperature may be kept nearly constant. The directions for the use of the apparatus accompany it. CHRISTENSEN'S and MYGGE'S method. 5 c. c. of urine, after being acidified with 2 drops of acetie acid, are poured into a some- what modified burette and precipitated with a certain quantity of a \% tannic-acid solution and then treated with 1 c. c. of mucilage. After the addition of water to a certain mark and after inverting the tube several times a uniform emulsion is produced. A cylin- drical glass filled one half or one third with water is now placed on a white surface having a number of close black lines traced upon it, and the contents of the burette are gradually added to the water with constant stirring, until by close observation the black lines cannot even be distinguished from the white spaces. The reading of the amount of urine emulsion employed gives directly the THE URINE. 401 amount of albumin in the urine; This method is claimed to give very good results. A special description accompanies each ap- paratus. The method proposed by ROBERTS and STOLNIKOW and further developed by BRANDBERG, though somewhat more difficult to perform, also gives satis- factory results. The density methods of LANG, HUPPERT, and ZAHOR are also very good. The last consists in determining the specific gravity before and after the coagulation of the albumins. Mucin occurs in the urine under normal conditions partly dissolved and partly in a strongly-distended, finely-divided state. It appears in greatest amounts in catarrhal affections of the urinary passages. The occurrence of pure mucin in the urine has thus far not been positively shown. To detect mucin in urine first dilute with water, partly to prevent the pre- cipitation of uric acid on the subsequent addition of acid, and partly to diminish the solubility of the mucin in the common salt of the urine. Now add an excess of acetic acid. The precipitate formed is purified by dissolving in water with the addition of a little alkali and reprecipitated with acetic acid. The precipitate is tested with the ordinary mucin reagents. To avoid mistak- ing mucin for nucleoalbumin, which is similar to mucin, the precipitate must be tested in regard to its behavior on boiling with dilute mineral acids. If no reducible substance is formed by this treatment, it contains no mucin. Blood and Blood-coloring Matters. The urine may contain blood from hemorrhage in the kidneys or other parts of the urinary passages (HJEMATUKIA). In these cases, when the quantity of blood is not very small, the urine is more or less cloudy and colored reddish, yellowish red, dirty red, brownish red, or dark brown. In recent hemorrhages, in which the blood has not decomposed, the color is nearer blood-red. Blood-corpuscles may be found in the sediment, sometimes also blood-cylinders and smaller or larger blood -clots. In certain cases the urine contains no blood-corpuscles, but only dissolved blood-coloring matters, haemoglobin, or, and indeed quite often, methaemoglobin (H^MOGLOBINTTRIA). The blood-coloring matters appear in the urine under different conditions, as in dissolu- tion of blood in poisoning with arseniuretted hydrogen, chlorates, etc., after serious burns, after transfusion of blood, and also in the periodic appearance of haemoglobinuria with fever. The urine may in haemoglobinuria also have an abundant grayish-brown sediment rich in albumin which contains the remains of the stromata of the red blood-corpuscles. In animals haemoglobinuria may be produced by many causes which force free haemoglobin into the plasma. To detect blood in the urine we make use of the microscope, 402 PHYSIOLOGICAL CUEMISTRT. spectroscope, the guaiacum test, and HELLER'S or HELLER- TEICHMANN'S test. Microscopic Investigation. The blood-corpuscles may remain undissolved for a long time in acid urine ; in alkaline urine, on the contrary, they are easily changed and dissolved. They often appear entirely unchanged in the sediment ; in some cases they are distended, and in others unequally pointed or jagged like a thorn- apple. In hemorrhage of the kidneys a cylindrical clot is some- times found in the sediment which is covered with numerous red blood-corpuscles, forming casts of the urinary passages. These formations are called BLOOD-CYLINDERS. The spectroscopic investigation is naturally of very great value; and if it be necessary to determine not only the presence but also the kind of coloring matter, this method is indispensable. In regard to the optical behavior of the various blood-coloring matters we must refer to Chapter IV. ALMEN'S Guaiacum Test. Mix in a test-tube equal volumes of tincture of guaiacum and old turpentine which has become strongly ozonized by the action of air under the influence of light. To this mixture, which must not have the slightest blue color, add the urine to be tested. In the presence of blood or blood-coloring matters, first a bluish-green and then a beautiful blue ring appears where the two liquids meet. On shaking the mixture it becomes more or less blue. Normal urine or one containing albumin does not give this reaction. For the reason of this we must refer the reader to Chapter IV, page 71. Urine containing pus, although no blood is present, gives a blue color with these reagents ; but in this case the tincture of guaiacum alone, without turpentine, is colored blue by the urine (VITALLI). This is at least true for a tincture that has been exposed for some time to the action of air and sunlight. The blue color produced by pus differs from that produced by blood -coloring matters by disappearing on heating the urine to boiling. A urine alkaline by decomposition must first be made faintly acid before performing the reaction. The turpentine should be kept exposed to sunlight, while the tincture of guaiacum must be kept in a dark glass bottle. These reagents to be of use must be controlled by a liquid containing blood. This test, it is true, in positive results is not absolutely decisive, because other bodies may THE URINE. 403 give a blue reaction ; but when properly performed it is so extremely delicate that when it gives negative results any other test for blood 'is superfluous. HELLEK-TEICHMANN'S Test. If a neutral or faintly-acid urine containing blood is heated to boiling, we always obtain a mottled precipitate consisting of albumin and haematin. If caustic soda is added to the boiling-hot test, the liquid becomes clear and turns green when examined in thin layers (due to haematin alkali), and a red precipitate, appearing green by reflected light, re-forms which consists of earthy phosphates and haBmatin. This reaction is called HELLER'S blood-test. If this precipitate is collected after a time on a small filter, it may be used for the haemin test (see page 79). If the precipitate contains only a little blood-coloring matter with a larger quantity of earthy phosphates, then wash it with dilute acetic acid, which dissolves the earthy phosphates, and use the residue for the preparation of TEICHMANN'S haemin crystals. If, on the contrary, the amount of phosphates is very small, then first add a little CaCl2 solution to the urine, heat to boiling, and add simultaneously with the caustic potash some sodium- phosphate solution. In the presence of only very small amounts of blood, first make the urine very faintly alkaline with ammonia, add tannic acid, acidify with acetic acid, and use the precipitate in the preparation of the haemin crystals (STKUVE). Melanin. In the presence of melanotic cancers sometimes dark coloring matters are eliminated with the urine. K. MORNER has isolated two coloring matters from such a urine, of which one was soluble in warm 50-75$ acetic acid and the other, on the contrary, was insoluble. The one seemed to be phymatorusin (see page 324). Usually the urine does not contain any melanin, but a chromogen of melanin, melanogen. In such cases the urine gives EISELT'S reaction, becoming dark-colored with oxidizing agents such as cone, nitric- acid, potassium bichromate, and sulphuric acid, as well as with free sulphuric acid. Urine containing melanin or melanogen is colored black by ferric- chloride solution (JAKSCH). BAUMSTARK found in a case of leprosy two characteristic coloring matters in the urine, ' ' urorubrohaematin " and " urofnscohaematin," which, as their names indicate, seem to stand in close relationship to the blood-coloring matters. Urorubrohwmatin, CssHa^tNsFesOae , contains iron and shows an absorption- band in front of D and one broader back of D. ID alkaline solution it shows four bands, behind D, at E, beyond Ft and behind G. It is not soluble either in water, alcohol, ether, or chloroform. It gives a beautiful brownish-red non-dichroitic liquid with alkalies. Urofuscoliamatin, CesHiosNsOaa , which is free from iron shows no characteristic spectrum ; it dissolves in alkalies, pro- ducing a brown color. Urorosein, so named by NENCKI. is a urinary coloring matter, occurring in various diseases, which appears on the acidification of the urine with a mineral acid, and which is taken up by shaking with amyl-alcohol. The solution shows an absorption-band between D and E. Uroerythrin, which gives a rose-red color to the urinary sediments especially in fevers, seems to occur also in urine under physiological conditions. It has not been thoroughly studied. A 404 PHYSIOLOGICAL CHEMISTRY. coloring matter nearly related to Ticematoporphyrin has been found by NEUSSER in two pathological urines. MACMUNN has found a coloring matter, uroTicema- tin, in rheumatism and ADDISON'S disease ; he also prepared it artificially from haematin. This coloring matter seems also to stand in close relationship to hsematoporphyrin. Pus occurs in the urine in different inflammatory affections, especially in catarrh of the bladder and in inflammation of the mem- brane of the kidneys or the urethra. Pus is best detected by means of the microscope. The pus-cells are rather easily destroyed in alkaline urines. In detecting pus we make use of DONNE'S pus-test, which is performed in the following way: Pour off the urine from the sediment as carefully as possible, place a small piece of caustic alkali on the sediment, and stir. If the pus-cells have not been previously changed, the sediment is converted by this means into a slimy tough mass. The pus-corpuscles swell up in alkaline urines, dissolve, or at least are so changed that they cannot be recognized under the mi- croscope. The urine in these cases is more or less slimy or fibrous, and it is precipitated in large flakes by acetic acid, so that it may possibly be mistaken for mucin. The closer investigation of the precipitate produced by acetic acid, and especially the appearance or non-appearance of a reducible substance after boiling it with a mineral acid, demonstrates the nature of the precipitated substance. Urine containing pus always contains albumin. Bile-acids. The statements in regard to the occurrence of bile- acids in the urine under physiological conditions do not agree. According to VOGEL and DRAGENDORFF and HONE, traces of bile- acids occur in the urine; according to HOPPE-SEYLER and v. UDRANSZKY, they do not. Pathologically they are present in the urine in hepatogenic icterus, although not always. Detection of Bile-acids in the urine. PETTENKOFER'S test gives always the most decisive reaction ; but as it gives similar color re- actions with other bodies, it must be supplemented by the spectro- scopic investigation. The direct test for bile-acids is easy after the addition of traces of bile to a normal urine. But the direct detec- tion in a colored icteric urine is more difficult and gives very mis- leading results; the bile-acid must therefore always be isolated from the urine. This may be done by the following method of HOPPE- SEYLER, which is slightly modified in non-essential points. HOPPE-SEYLER'S METHOD. Strongly concentrate the urine, and extract the residue with strong alcohol. The filtrate is freed from alcohol by evaporation and then precipitated by basic lead acetate and ammonia. The washed precipitate is treated with boiling al- cohol, filtered hot, the filtrate treated with a few drops of soda solu- -THE URINE. 405 tion, and evaporated to dryness. The dry residue is extracted with absolute alcohol, filtered, and an excess of ether added. The amor- phous or, after a longer time, crystalline precipitate consisting of alkali-salts of the biliary acids is used in performing PETTEN- KOFER'S test. Bile-coloring matters occur in the urine in different forms of icterus. A urine containing bile-coloring matters is always abnor- mally colored — yellow, yellowish brown, deep brown, greenish yellow, greenish brown, or nearly pure green. On shaking it froths, and the bubbles are yellow or yellowish green in color. As a rule icteric urine is somewhat cloudy, and the sediment is frequently, especially when it contains epithelium-cells, rather strongly colored by the bile-coloring matters. In regard to the occurrence of urobilin in icteric urine see page 371. Detection of Bile-coloring Matters in urine. Many tests have been proposed for the detection of bile-coloring matters. Ordina- rily we obtain the best results either with GMELIN'S or with HUP- PERT'S test. GMELIN'S test may be applied directly to the urine; but it is better to use ROSENBACH'S modification. Filter the urine, which is deep-colored from the retained epithelium-cells and bodies of that kind, through a very small filter. After the liquid has entirely passed through apply to the inside of the filter a drop of nitric acid which contains only very little nitrous acid. A pale-yellow spot will be formed which is surrounded by colored rings which appear yellow- ish red, violet, blue, and green. This modification is very delicate, and* it is hardly possible to mistake indican and other coloring mat- ters for the bile-pigments. Several other modifications of GMELIN'S test on the urine directly, as with concentrated sulphuric acid and nitrate, etc., have been proposed, but they are neither simpler nor more accurate than ROSENBACH'S modification. HUPPERT'S Reaction. In a dark-colored urine or one rich in indican we do not always obtain good results with GMELIN'S test. In such cases, as also in urines containing blood-coloring matters at the same time, the urine is treated with lime-water, or first with some CaCl2 solution, and then with a solution of soda or ammonium carbonate. The precipitate which contains the bile-coloring mat- ters is filtered and used for HUPPERT'S test (see page 154). The precipitate consisting of lime-pigments may also be shaken out with chloroform after washing in water and after being acidified with acetic acid. The bilirubin is taken up by the chloroform, which is colored yellow thereby, while the acetic acid solution is colored green by the bilirubin. Both solutions may then be used 406 PHYSIOLOGICAL CHEMISTRY. for GMELIN'S test (HoppE-SEYLER),and small amounts of bile-color- ing matters may be detected in this way. The lime-pigments may, according to HILGER, also be used directly for GMELIN'S test in the following way : Spread them on a porcelain dish in a thin layer, and add carefully a drop of nitric acid. The reaction generally appears very beautiful. STOKVIS'S reaction is especially valuable in those cases in which the urine contains only very little bile-coloring matter together with larger amounts of other coloring matters. The test is performed as follows : 20-30 c. c. urine are treated with 5-10 c. c. of a solu- tion of zinc acetate (1 : 5). The precipitate is washed on a small filter with water and then dissolved in a little ammonia. The new filtrate gives, directly or after it has stood a short time in the air until it has a peculiar brownish-green color, the absorption-bands of bilicyanin (see page 155). Many other reactions for bile-coloring matters in the urine have been proposed; but as the above-mentioned are sufficient, it is per- haps only necessary to give here a few of the other reactions with- out entering into details. ULTZMANN'S reaction consists in treating about 10 c. c. of the urine with 3-4 c. c. concentrated caustic-potash solution and then acidifying with hydro- chloric acid. The urine will become a beautiful green. SMITH'S Reaction. Pour carefully over the urine tincture of iodine, whereby a green ring appears between the two liquids. You may also shake the urine with tincture of iodine until it has a green color. EHRLICH'S Test. First mix the urine with an equal volume of dilute acetic acid and then add drop by drop a solution of sulpho-diazobenzol. The acid mixture becomes dark red in the presence of bilirubin, and this color becomes bluish violet on the addition of glacial acetic acid. The sulpho-diazobenzol is prepared with 1 grm. sulphanilic acid, 15 c. c. hydrochloric acid, and* 0.1 grm. sodium nitrite ; this solution is diluted to 1 litre with water. MEDICINAL COLORING MATTERS produced from santonin, rhubarb, senua, etc. , may give an abnormal color to the urine, which may be mistaken for the bile-coloring matters, or in alkaline urines perhaps for blood-coloring matters. If hydrochloric acid is added to such a urine, it becomes yellow or pale yellow, while on the addition of an excess of alkali it becomes more or less beautifully red. Sugar in Urine. Grape-sugar, C6H1206, also called GLUCOSE, DEXTROSE, and DIABETIC sugar, occurs chiefly in the vegetable kingdom; but it is found in very small amounts in the blood, on an average of 1.5 p. m., and also as traces in other animal fluids and organs. The occurrence of traces of grape-sugar in the urine of perfectly healthy persons can hardly be disputed. If sugar appears in the urine in constant THE URINE. 407 and in specially large amounts, it must be considered as an abnor- mal constituent. Small amounts of glucose may pass into the urine from an ex- cessive supply of sugar when the body, by absorption from the intestines, takes up more sugar than it can assimilate (WORM MULLER; F. HOFMEISTER, DE JONG). Also after starchy food HOFMEISTER observed in dogs, whose power of assimilating sugar had been greatly reduced by the almost complete withdrawal of food for several days, that glycosuria appeared (STARVATION-DIA- BETES according to HOFMEISTER). In man the appearance of glucose in the urine has been observed in numerous and various pathological conditions, such as lesions of the brain and especially of the medulla oblongata, abnormal circulation in the abdomen, heart and lung diseases, cirrhosis of the liver, cholera, etc., etc. Glycosuria has also been produced in animals in various ways, as by puncture of the fourth ventricle (piqure), cutting through the spinal marrow, irritation of the pneumogastric nerve, poisoning with carbon monoxide, curare, amyl-nitrite, o-nitro-phenyl-propiolic acid, phloridzin, and many other substances, also by the injection of dilute common-salt solution into the blood-vessels, and in many other ways. The observation of v. MERING and MINKOWSKI that in dogs after total extirpation of the pancreas a very copious and continuous secretion of sugar, a true diabetes, appears is of special interest. The continuous appearance of sugar in human urine, sometimes in very considerable amounts, occurs in DIABETES MELLITUS. In this disease there may be an elimination of 1 kilogramme of grape- sugar during the 24 hours, or even more. In the beginning of the disease, when the quantity of sugar is still very small, the urine often does not appear abnormal. In more developed, typical cases the quantity of urine voided increases considerably to 3-6-10 litres per 24 hours. The percentage of the physiological constituents is as a rule very low, while their absolute daily quantity is increased. The urine is pale, but of a high specific gravity, 1.030-1.040 or even higher. The high specific gravity depends upon the sugar present, which varies in different cases, but may even amount to The urine is therefore characterized in typical cases of dia- 408 PHYSIOLOGICAL CHEMISTRY. betes by the very large quantity voided, by the pale color and high specific gravity, and by its containing sugar. That the urine after the introduction of certain medicines or poisonous bodies into the system contains reducing bodies, paired glycuronic acids, which may be mistaken for sugar, has been men- tioned in the previous pages. Properties of Grape-sugar. Grape-sugar crystallizes sometimes with 1 mol. water of crystallization in warty masses or small leaves or plates, and sometimes when free from water in needles. The sugar containing water of crystallization melts even below 100° C. and loses its water of crystallization at 110° C. The anhydrous sugar melts at 146° C. and is converted into glucosan, C6H1006, at 170° C. with the elimination of water. On strongly heating it is converted into caramel and then decomposed. Grape-sugar is readily soluble in water. This solution, which is not as sweet as a cane-sugar solution of the same strength, is dextro-gyrate. The specific rotary power of a watery solution of 1-15$ anhydrous glucose at -|- 20° C. is indeed somewhat variable, 52.52°-52.9° (LANDOLT), but the average may be considered as -f- 52.6°. Anhydrous glucose dissolves sparingly in cold, but more freely in boiling, alcohol. 100 parts 85$ alcohol dissolves 1.94 parts glucose at -f- 17.5° C. and 21.7 parts at the boiling tempera- ture (ANTHON). Glucose is insoluble in ether. If an alcoholic caustic-alkali solution is added to an alcoholic solution of glucose, an amorphous precipitate of insoluble saccharate is formed. On warming the saccharate decomposes easily with the formation of a yellow or brownish color which is the basis of the following re- action. MOORE'S Test. If a glucose solution is treated with about £ of its volume of caustic potash or soda and warmed, the solution be- comes first yellow, then orange, yellowish brown, and lastly dark brown. It has at the same time a faint odor of caramel, and this odor is more pronounced on acidification. Glucose forms many crystallizable combinations with NaCl, of which the easiest to obtain is (06H1206)8.NaCl + H20, which forms large colorless six-sided double pyramids or rhomboids with 13.40$ NaOl. Glucose in neutral or very faintly-acid (by an organic acid) THE URINE. 409 solution passes into alcoholic fermentation with beer -yeast, C'6H1206 = 2C2H5.OH + 2C02. The most favorable temperature for this fermentation is about 25° C. Besides the alcohol and 'carbon dioxide there are formed, especially at higher temperatures, small quantities of homologous alcohols (amyl-alcohol), glycerin, and succinic acid. In the presence of acid milk or cheese the grape- sugar passes, especially in the presence of a base such as ZnO or CaC03, into lactic-acid fermentation: C6H1206 = 2C3H603. (This process is even more complicated and C02 is formed thereby, Bou- TROtf, HUEPPE.) The lactic acid may then pass into butyric acid- fermentation : 2C3H603 = C4H802 + 2C02 + 4H. Grape-sugar reduces several metallic oxides, such as copper oxide, bismuth oxide, mercuric oxide, in alkaline solutions, and the most important reactions for sugar are based on this fact. TROMMER'S test is based on the property that glucose possesses of reducing copper-hydrated oxide in alkaline solution into sub- oxide. Treat the glucose solution with about £-J vol. caustic soda and then carefully add a dilute copper-sulphate solution. The copper-hydrated oxide is thereby dissolved, forming a beautiful blue solution, and the addition of copper sulphate is continued until a very small amount of hydrate remains undissolved in the liquid. This is now warmed and a yellow hydrated suboxide or red suboxide separates even below the boiling-point. If too little copper salt has been added, the test will be yellowish brown in color as in MOORE'S test; but if an excess of copper-salt has been added, the excess of hydrate is converted on boiling into a dark-brown hydrate poor in water which interferes with the test. To prevent these difficulties the so-called FEELING'S solution may be em- ployed. This reagent is obtained by mixing before use equal volumes of an alkaline solution of Rochelle salts and a copper- sul- phate solution (see Quantitative Estimation of Sugar in the Urine in regard to concentration). This solution is not reduced or noticeably changed by boiling. The tartrate holds the excess of copper- hydrate oxide in solution, and an excess of the reagent does not interfere in the performance of the test. In the presence of sugar this solution is reduced. BOTTGER-ALMEN'S test is based on the property glucose possesses of reducing bismuth oxide in alkaline solution. The reagent best 410 PHYSIOLOGICAL CHEMISTRY. adapted for this purpose is obtained, according to NYLANDER'S modification of ALMEN'S original test, by dissolving 4 grms. Rochelle salt in 100 parts caustic soda of 10$ NaHO and adding 2 grms. bismuth subnitrate and digesting on the water-bath until as much of the bismuth salt is dissolved as possible. If a glucose solution is treated with about -fa vol., or with a larger quantity of the solution when large quantities of sugar are present, and boiled for a few minutes, the solution becomes first yellow, then yellowish brown, and lastly nearly black, and after a time a black deposit of bismuth (?) settles. On heating with PHENYLHYDRAZIN a grape-sugar solution gives a precipitate consisting of fine yellow crystalline needles which are nearly insoluble in water but soluble in boiling alcohol, and which separate again on treating the alcoholic solution with water. The crystalline precipitate consists of plienylglucosazom (E. FISCHER) : C6H1206 + 2C6H6.N2H3 = O^H^NA + 2H20 + 2H. This compound melts when pure at 204°-205° C. Glucose is not precipitated by a lead-acetate solution, but is almost completely precipitated by an ammoniacal basic lead-acetate solution, On warming the precipitate becomes flesh-color or rose- red. If a watery solution of grape-sugar is treated with BENZOYL- CHLORIDE and an excess of caustic soda and shaken until the odor of benzoylchloride has disappeared, a precipitate of benzoic-acid ester of glycose will be produced which is insoluble in water or alkali (BAUMANtf). If -J-l c. c. of a dilute watery solution of glucose is treated with a few drops of a 15$ alcoholic solution of a-naphthol, the liquid is colored a beautiful violet on the addition of 1-2 c. c. concentrated sulphuric acid (MOLISCH). This reaction depends on the formation of furfurol from the sugar by the action of the sulphuric acid. DIAZOBENZOL-SULPHONIC ACID gives with a sugar solution made alkaline with a fixed alkali a red color gradually changing after 10-15 minutes to violet (PENZOLDT and FISCHER). ORTHONITROPHENYL-PROPIOLIC ACID yields indigo when boiled with a small quantity of sugar and sodium car- bonate, and this is converted into indigo-white by an excess of sugar (BAEYER). An alkaline solution of grape-sugar is colored deep red on being warmed with a dilute solution of PICRIC ACID (BRANN). The detection of sugar in urine is ordinarily, in the presence of not too little sugar, a very simple opperation. The presence of THE URINE. 411 only very small amounts may make its detection sometimes very difficult and laborious. A urine containing albumin must first have the albumin removed by coagulation with acetic acid and heat before it can be tested for sugar. The tests which are most frequently employed and are especially recommended are as follows: TROMMER'S test. In a typical diabetic urine or one rich in sugar this test succeeds well, and it may be performed in the manner suggested on page 409. This test may lead to very great mistakes in urines poor in sugar, especially when they have at the same time normal or increased amounts of physiological constitu- ents, and therefore it cannot be recommended to physicians or to persons little practised in such work. Normal urine contains reducible substances, such as uric acid, creatinin, and others, and therefore a reduction takes place with all urine on using this test. We generally do not have a separation of copper suboxide, but still if we vary the proportion of the alkali to the copper sulphate and boil, we often have an actual separation of suboxide in normal urines, or we obtain a peculiar yellowish-red liquid due to finely- divided hydrated suboxide. This occurs especially on the addition of much alkali or too much copper sulphate, and by careless ma- nipulation the inexperienced worker may therefore sometimes obtain apparently positive results in a normal urine. Also as urine contains substances such as creatinin and ammonia (from the urea), which in the presence of only little sugar may keep the cop- per suboxide in solution, therefore in inexperienced hands small amounts of sugar may be overlooked. TROMMER'S test may indeed be made positive and useful, even in the presence of very small amounts of sugar, by using the modifica- tion suggested by WORM MULLER. As this modification is rather complicated, and besides this requires much practice and exactness, it is probably rarely employed by the busy physician. The follow- ing test is to be preferred : ALMEN'S bismuth test, which recently has been incorrectly called NYLANDER'S test, is performed with the alkaline bismuth solution prepared as above described (page 410). For each test 10 c. c. of urine are taken and treated with 1 c. c. of the bismuth solution and boiled for a few minutes. In the presence of sugar the urine becomes darker yellow or yellowish brown. Then it grows darker^ cloudy, dark brown, or nearly black, and non-transparent. After a shorter or longer time a black deposit appears, the supernatant liquid gradually clears, but still remains colored. In the presence of only very little sugar the test is not black or dark brown, but is only deeper-colored, and after a longer time we only see on the upper layer of the phosphate precipitate a dark or black edge (of bismuth ?). In the presence of much sugar a larger amount of 412 PHYSIOLOGICAL CHEMISTRY. reagent may be used without disadvantage. In a urine poor in sugar we must only use 1 c. c. of the reagent for every 10 c. c. of the urine. This test shows the presence of 1-0. 5 p. m. sugar in the urine. The sources of error which interfere in TROMMER'S test, such as the presence of uric acid and creatinin, entirely disappear in this test. The bismuth test is, besides this, more easily performed, and it is therefore to be recommended to the physician. Small amounts of albumen do not interfere with this test ; large amounts may give rise to an error by forming bismuth sulphide, and therefore must be removed by coagulation. In using this method it must not be overlooked that it is, like TROMMER'S test, a reduction test, and it consequently may show, besides sugar, certain other reducing substances. Such bodies are certain coupled glycuronic acids which may appear in the urine. SALKOWSKI obtained a bluish-black precipitate with this reagent in a urine after the use of rhubarb, and black precipitates have been obtained with the bismuth test after the use of turpentine and cer- tain other medicines. From this it follows that we should, espe- cially when the reduction is not very great, never be satisfied with this test alone. As a control at least one of the following tests must be performed. Among these the fermentation test is of special value. Fermentation Test. On using this test we must proceed in vari- ous ways, according as the bismuth test gives small or large results. If a rather strong reduction is obtained, the urine may be treated with yeast and the presence of sugar determined by the generation of carbon dioxide. In this case the acid urine, or otherwise faintly acidified with tartaric acid, is treated with yeast which has previ- ously been washed by decantation with water. Pour this urine to which the yeast has been added into a SCHROTTER'S gas-burette, or glass tube with the open end ground, close with the thumb, and open under the surface of mercury contained in a dish. As the fermentation proceeds, the carbon dioxide collects in the upper part of the tube/while a corresponding quantity of liquid is expelled below. As a control in this case, two other similar tests must be made, one with normal urine and yeast to learn the amount of gas usually developed, and the other with a sugar solution and yeast to determine the activity of the yeast. If, on the contrary, we find only a faint reduction with the bis- muth test, no positive conclusion can be drawn from the absence of any carbon dioxide or the appearance of a very insignificant amount. In this case proceed in the following way : Treat the acid urine, or the urine which has been faintly acidified with tar- taric acid, with yeast whose activity has been tested by a special test on a sugar solution, and allow it to stand 24-48 hours at the THE URINE. 413 temperature of the room, or, better, at a little higher temperature. After this time test again with the bismuth test, and if the reac- tion now gives negative results, then sugar was previously present. But if the reaction continues to give positive results, then it shows — if the yeast is active — the presence of other reducing, ferment- able bodies. There may indeed be a possibility that the urine also contains some sugar besides these bodies. This possibility is decided by the following test : Phenylhydrazin Test. According to v. JAKSCH, this test is per- formed in the following way : Add in a test-tube containing 8-10 c. c. of the urine two knife-points of phenylhydrazin hydrochloride and three knife-points sodium acetate, and when the added salts do not dissolve on warming add more water. The mixture is heated in boiling water and kept there for one hour to avoid a confusion with phenylhydrazin-glycuronic acid (v. JAKSCH and HIRSCHL). It is then poured into a beaker of cold water. If the quantity of sugar present is not too small, a yellow crystalline precipitate is now obtained. If the precipitate appears amorphous, then on looking at it under the microscope it appears partly as single and partly as groups of yellow needles. If very little sugar is present, pour the test into a conical glass and examine the sediment. In this case at least a few phenylglucosazone crystals are found, while the occurrence of smaller and larger yellow plates or highly-refrac- tive brown globules do not show the presence of sugar. Accord- ing to v. JAKSCH, this reaction is very reliable, and by it the presence of 0.3 p. m. sugar can be detected (ROSENBERG, G-EYER). A confounding with glycuronic acid is, according to HIKSCHL, not to be apprehended when it is not heated in the water-bath for too short a time (one hour). In doubtful cases where we wish to be quite sure, prepare the crystals from a large quantity of urine, dis- solve them on the filter by pouring over them hot alcohol, treat the filtrate with water, and boil off the alcohol. If the characteristic yellow crystalline needles, whose melting-point (204°-205° C.) is also determined, are now obtained, then this test is quite decisive. Polarization. The polariscopic investigation is of great value, especially as in many cases it quickly differentiates between sugar and other reducible, often laevo-gyrate substances. In the presence of only very little sugar the value of this test depends on the delicacy of the instrument and the dexterity of the observer; therefore this method is perhaps inferior in most cases to the bismuth test or to the phenylhydrazin test. If small quantities of sugar are to be isolated from the urine, precipitate the urine first with sugar of lead, filter, precipitate the filtrate with ammoniacal lead acetate, wash this precipitate with water, decompose it with H2S when suspended in water, concentrate the filtrate, treat it with strong alcohol until it is 80 vol. per cent, filter 414 PHYSIOLOGICAL CHEMISTRY. when necessary, and add an alcoholic caustic-alkali solution. Dis- solve the precipitate consisting of saccharates in a little water, pre- cipitate the potash by an excess of tartaric acid, neutralize the fil- trate with calcium carbonate in the cold, and filter. The filtrate may be used for testing with the polariscope as well as in the fer- mentation, bismuth, and phenylhydrazin tests. The presence of grape-sugar may be detected by this same process in animal fluids or tissues, from which the albumins have first been removed by coagulation or by the addition of alcohol. For the physician, who naturally wants specially simple and quick methods, the bismuth test must be especially recommended, as this may be controlled when necessary by the fermentation or phenylhydrazin test. Other tests for sugar, as, for example, the reaction with orthonitrophenyl- propiolic acid, picric acid, diazobeuzol-sulphonic acid, are superfluous. The reaction with a-naphthol, which is a reaction for carbohydrates in general, for glycuronic acid and uiucin, may, because of its extreme delicacy, give rise to mistakes, and is therefore not to be recommended to physicians. Quantitative Estimation of Sugar in the urine. The urine for such an estimation must first be tested for albumin, and if any be present it must be removed by coagulation and the addition of acetic acid, care being taken not to increase or diminish the original volume of urine. The quantity of sugar may be determined by TITRATION with FEHLING'S or KNAPP'S solution, by FERMENTA- TION or by POLARIZATION. The titration liquids not only react with sugar, but also with certain other reducing substances, and on this account the titration methods give rather high results. When large quantities of sugar are present, as in typical diabetic urine, which generally contains a lower percentage of normal reducing constituents, this is indeed of little account ; but when small amounts of sugar are present in an otherwise normal urine, the mistake may, on the contrary, be • important, as the reducing power of normal urine may correspond to 4 p. m. grape-sugar (see page 374). In such cases the titration method must be employed in connection with the fermentation method, which will be described later. It is to be remarked that in typical diabetic urines with considerable amounts of sugar the titration with FEELING'S solution is just as reliable as with KNAPP'S solution. When the urine, on the contrary, contains only little sugar with normal amounts of physiological constituents, then the titration with FEHLING'S solution is more difficult, indeed in cer- tain cases almost impossible, the results being very uncertain. In such cases KNAPP'S method gives good results, according to WORM MULLER and his pupils. The TITRATION with FEHLING'S SOLUTION depends on the power of sugar to reduce copper oxide in alkaline solutions. For this we THE formerly employed a solution which contained a mixture of Copper sulphate, Rochelle salt, and sodium or potassium hydrate (FEH- LING'S solution) ; but as such a solution readily changes, we now prepare a copper-sulphate solution and an alkaline Kochelle-salt solution separately, and mix equal volumes of the two solutions before using. The concentration of the copper-sulphate solution is such that 10 c. c. of this solution is reduced by 0.05 grm. grape-sugar. The copper-sulphate solution contains 34.65 grms. pure, crystallized, non- efflorescent copper sulphate in 1 litre. The sulphate is crystallized from a hot saturated solution by cooling and stirring; and the crystals are separated from the mother-liquor and pressed between blotting-paper until dry. The Rochelle-salt solution is prepared by dissolving 173 grms. of the salt in 350 c. c. water, adding 600 c. c. of a caustic-soda solution of a specific gravity of 1.12, and dilut- ing with water to 1 litre. According to WORM MULLER, these three liquids — Rochelle-salt solution, caustic soda, and water — should be separately boiled before mixing together. For each titration mix in a small flask or porcelain dish exactly 10 c. c. of the copper-sulphate solution and 10 c. c. of the alkaline Rochelle- salt solution and add 30 c. c. water. The urine free from albumin is diluted before the titration with water so that 10 c. c. of the copper solution requires between 5 and 10 c. c. of the diluted urine, which corresponds to between 1 and \% sugar. A urine of a specific gravity of 1.030 may be diluted five times; one more concentrated, ten times. The urine so diluted is poured into a burette and allowed to flow into the boiling copper-sulphate and Rochelle-salt solution until the copper oxide is completely reduced. This has taken place when, after boiling, the blue color of the solution disappears. It is very dif- ficult and requires some practice to exactly determine this point, especially when the copper suboxide settles with difficulty. To determine whether the color has disappeared, allow the copper suboxide to settle a little below the meniscus formed by the surface of the liquid. If this layer is not blue, the operation is repeated, adding 0.1 c. c. less of urine; and if, after the copper suboxide has settled, the liquid has a blue color, the titration may be considered as completed. Because of the difficulty in obtaining this point exactly another end-reaction has been suggested. This consists in filtering immediately after boiling a small portion of the treated urine through a small filter into a test-tube which contains a little acetic acid and a few drops of potassium-ferrocyanide solution and water. The smallest quantity of copper is shown by a red colora- tion. If the operation is quickly conducted so that no oxidation of the suboxide into oxide takes place, this end-reaction is of value for urines which are rich in sugar and poor in urea and which 416 PHYSIOLOGICAL CHEMISTRY. have been ' strongly diluted with water. In urines poor in sugar which contain the normal amount of urea and which have not been strongly diluted, a rather abundant formation of ammonia from the urea may take place on boiling the alkaline liquid. This am- monia dissolves the suboxide in part, which easily passes into oxide thereby, and besides this the dissolved suboxide gives a red color with potassium ferrocyanide. In just those cases in which the titration is most difficult this end-reaction is the least reliable. Practice also renders it unnecessary, and it is therefore best to depend simply upon the appearance of the liquid. To facilitate the settling of the copper suboxide and thereby clearing the liquid, MUNK has lately suggested the addition of a little calcium-chloride solution and boiling again. A precipitate of calcium tartrate is produced which carries down the suspended copper suboxide with it, and the color of the liquid can then be better seen. This artifice succeeds in many cases, but unfortunately there are urines in which the titration with FEHLING'S solution in no way gives exact results. The necessary conditions for the success of the titration under all circumstances are, according to SOXHLET, the following: The copper-sulphate and Rochelle-salt solution must, as above, be diluted to 50 c. c. with water; the urine must only contain between 0.5$ and \% sugar, and the total quantity of urine required for the re- duction must be added to the titration liquid at once and boiled with it. From this last condition it follows that the titration is dependent upon minute details, and several titrations are required for each determination. It is best to give here directions for the titration. The proper amount of copper-sulphate and Rochelle-salt solution and water (total volume = 50 c. c.) is heated to boiling in a flask: the color must remain blue. The urine diluted five times is now added to the boiling-hot liquid, 1 c. c. at a time; after each addition of urine boil for a few seconds, and look for the appearance of the end-reaction. If you find, for example, that 3 c. c. is too little but that 4 c. c. is too much (the liquid becoming yellowish), then the urine has not been sufficiently diluted, for it should require be- tween 5 and 10 c. c. of the urine to produce the complete reduc- tion. The urine is now diluted ten times, and it should now require between 6 and 8 c. c. for a total reduction. Now prepare four new tests, which are boiled simultaneously to save time, and add at one time respectively 6, 6£, 7, and 7J- c. c. of urine. If it is found that between 6J and 7 c. c. are necessary to produce the end-reaction, then make four other tests, to which add respectively 6.6, 6.7,6.8, and 6.9 c. c. of urine. If in this case the liquid is still somewhat bluish with 6.7 c. c. and completely decolorized with 6.8 c. c., we then consider the average figure 6.75 c. c. as correct. THE URINE. 417 The calculation is simple. The 6.75 c. c. used contain 0.05 grm. sugar, and the percentage of sugar in the dilute urine is therefore (6.75 : 0.05 = 100 : x) = ^— = 0.74. But as the urine was 0. i O diluted with ten times its volume of water, the undiluted urine contained —£-=•£- = 7.4$. The general formula on using 10 c. c. o. i o copper-sulphate solution is therefore — r — , in which n represents the number of times the urine has been diluted and k the number of c. c. used for the titration of the diluted urine. The TITRATION ACCORDING TO KN APP depends on the fact that mercuric cyanide is reduced into metallic mercury by grape-sugar. The titration liquid should contain 10 grms. chemically pure dry mercuric cyanide and 100 c. c. caustic-soda solution of a specific gravity of 1.145 per litre. When the titration is performed as described below (according to WORM MULLER and OTTO), 20 c. c. of this solution should correspond to exactly 0.05 grm. grape-sugar. Also in this titration the quantity of sugar in the urine should be between \% and 1%, and here also the extent of dilution necessary must be determined by a preliminary test. To determine the end- reaction as described below, the test for excess of mercury is made with sulphuretted hydrogen. In performing the titration allow 20 c. c. of KNAPP'S solution to flow into a flask and dilute with 80 c. c. water, or when you have reason to think that the urine contains less than 0.5$ of sugar, then only with 40-60 c. c. After this heat to boiling and allow the dilute urine to flow gradually into the hot solution, at first 2 c. c., then 1 c. c., then 0.5 c. c., then 0.2 c. c., and lastly 0.1 c. c. After each addition let it boil J minute. When the end-reaction is approaching, the liquid begins to clarify and the mercury separates with the phosphates. The end-reaction is determined by taking a drop of the upper layer of the liquid into a capillary tube and then blowing it out on pure white filter-paper. The moist spot is first held over a bottle containing fuming hydrochloric acid and then over strong sulphuretted hydrogen. The presence of a minimum quantity of mercury-salt in the liquid is shown by the spot becom- ing yellowish, which is seen best when it is compared with a second spot which has not been exposed to sulphuretted hydrogen. The end-reaction is still clearer when a small part of the liquid is filtered, acidified with acetic acid, and tested with sulphuretted hydrogen (OTTO). The calculations are just as simple as for the previous method. This titration, unlike the previous one, may be performed not only in daylight, but also in artificial light. KNAPP'S method has 418 PHYSIOLOGICAL CHEM18THY. the following advantages over FEELING'S method. It is applicable even when the quantity of sugar in the urine is very small and the amount of the other urinary constituents is normal. It is more easily performed, and the titration liquids may be kept without decomposing for a long time (WORM MULLER and his pupils). The views of different investigators on the value of this titration method are still somewhat contradictory. ESTIMATION OF THE QUANTITY OF SUGAR BY FERMENTATION. This may be done in various ways ; the simplest, and at the same time sufficiently exact for ordinary cases, is ROBERTS' method. This method consists in determining the specific gravity of the urine before and after fermentation. In the fermentation of sugar, carbon dioxide and alcohol are formed as chief products and the specific gravity is lowered, partly on account of the disappearance of the sugar and partly on account of the production of alcohol. EGBERTS found, and this has been substantiated later by several other investigators (WORM MULLER and others), that a decrease of 0.001 in the specific gravity corresponded to 0.23$ sugar. If the urine, for example, had a specific gravity of 1.030 before fermenta- tion and 1.008 after, then the quantity of sugar contained therein was 22 x 0.23 = 5.06$. In performing this test the specific gravity must be taken at the same temperature before and after the fermentation. The urine must be faintly acid, and when necessary it should be acidified with a little tartaric-acid solution. The activity of th'e yeast must, when necessary, be controlled by a special test. Place 200 c. c. of the urine in a 400-c. c. flask and add a piece of compressed yeast the size of a pea, and subdivide the yeast through the liquid by shak- ing, close the flask with a stopper provided with a finely drawn-out open glass tube, and allow the test to stand at the temperature of the room, or still better at + 20-25° C. After 24-48 hours the fermentation is. ordinarily ended, but this must be verified by the bismuth test. After complete fermentation filter through a dry filter, bring the filtrate to the proper temperature, and determine the specific gravity. If the specific gravity be determined with a good pyknometer supplied with a thermometer and an expansion-tube, this method, when the quantity of sugar is not less than 4-5 p. m., gives, accord- ing to WORM MULLER, very exact results, but this has been disputed by BUDDE. For the physician this method in this form is not serviceable. Even if the specific gravity is determined by a delicate urinometer which can give the density to the fourth decimal, we do not obtain quite exact results because of the principal errors of the method (BUDDE) ; but the error is usually smaller than those which occur in ti'trations made by unpractised hands. Among the methods proposed and closely tested for the quantitative esti- THE URINE. 419 mation of sugar, we have none which are at the same time easily performed and which give positive results in other than practised hands. When the quantity of sugar is less than 5 p. m. these methods cannot be used. Such a small amount of sugar cannot, as above mentioned, be determined by titration directly, because the reduc- tion power of normal urine corresponds to 4-5 p. m. In such cases, according to WORM MULLER, first determine the reduction power of the urine by titration with K^APP'S solution, then ferment the urine with the addition of yeast, and titrate again with KNAPP'S solution. The difference found between the two titrations calcu- lated as sugar gives the true amount of sugar. ESTIMATION OF SUGAR BY POLARIZATION. In this method the urine must be clear, not too deeply colored, and, above all, must not contain any other optically-acting substances besides glucose. By using a delicate instrument and with sufficient practice very exact results can be obtained by this method (K. MORNER, H. HUPPERT). For the physician, ROBERTS' fermentation test, which requires no expensive apparatus and no special practice, is to be pre- ferred. Under such circumstances, and as the estimation by means of polarization can be performed with exactitude only by specially- instructed chemists, it is hardly necessary to give this method in detail, and the reader is referred to special works for instructions in the use of the apparatus. Levulose. Lsevo-gyrate urines containing sugar have been observed by VENTZKE, ZIMMER and CZAPEK, SEEGEN, and others. The nature of the sub- stance causing this action is difficult to exactly describe, but there is hardly any doubt that the urine, at least in certain cases, as in those observed by SEEGEN, contains levulose. LEO once found in a diabetic urine a laevo-gyrate, reduci- ble, non-fermentable, and non-crystallizable substance which was considered by him as a peculiar variety of sugar. The presence of levulose in a urine containing sugar is only probable when the urine is laevo-gyrate or optically inactive, or when it shows a reduction power not corresponding (less) to the dextro-rotary power, or when it contains no other Isevo-gyrate substances (/?-oxybutyric acid, coupled glycuronic acids, protein bodies, or cystin). MILK-SUGAR. The appearance of milk-sugar in the urine of nursing mothers has been made known especially by the investiga- tions of DE SINETY and F. HOFMEISTER. After taking large quan- tities of milk-sugar some lactose was found in the urine by WORM MULLER, and DE JONG found also glucose under the same circum- stances. The positive detection of milk-sugar in the urine is difficult, be- cause this sugar is, like glucose, dextro-gyrate and also gives the usual reduction tests. If urine contains a dextro-gyrate, non-fer- 420 PHYSIOLOGICAL CHEMISTRY. men table sugar which reduces bismuth solutions, then it is very probable that it contains milk-sugar. The most certain means for its detection is to isolate the sugar from the urine. This may be done by the following method, suggested by F. HOFMEISTEE. Precipitate the urine with sugar of lead, filter, wash with water, unite the fitrate and wash-water, and precipitate with ammonia. The liquid filtered from the precipitate is again precipitated by sugar of lead and ammonia, until the last filtrate is optically inactive. The several precipitates with the excep- tion of the first, which contains no sugar, are united and washed with water. The washed precipitate is decomposed in the cold with sulphuretted hydro- gen and filtered. The excess of sulphuretted hydrogen is driven off by a current of air ; the acids set free are removed by shaking with silver oxide. Now filter, remove the dissolved silver by sulphuretted hydrogen, treat with barium carbonate to unite with any free acetic acid present, aud concentrate. Before the evaporated residue is syrupy it is treated with 90$ alcohol until a flocculent precipitate is formed which settles quickly. The filtrate from this when placed in a desiccator deposits crystals of milk-sugar, which are purified by recrystallization, decolorizing with animal charcoal and boiling with 60- 70$ alcohol. INOSIT occurs only rarely, and only in small quantities, in the urine in albuminuria and in diabetes mellitus. After excessive drinking of water inosit is found in the urine. According to HOPPE-SEYLER, traces of inosit occur in all normal urines. In detecting inosit the albumin is first removed from the urine. Then concentrate the urine on the water-bath to £ and precipitate with sugar of lead. The filtrate is warmed and treated with lead acetate as long as a precipitate is formed. The precipitate formed after 24 hours is washed with water, suspended in water, and de- composed with sulphuretted hydrogen. A little uric acid may separate from the filtrate after a short time. The liquid is filtered, concentrated to a syrupy consistency, and treated while boiling with 3-4 vols. alcohol. The precipitate is quickly separated. After the addition of ether to the cooled filtrate, crystals separate after a time, and these are purified by decolorization and 'recrystallization. with these crystals perform the tests mentioned on page 258. Aceton and Aceto-acetic Acid. These bodies were first observed in urine in diabetes mellitus (PETERS, KAULICH, v. JAKSCH, GER- HARDT). Both of these bodies occur sometimes simultaneously in diabetic urine, sometimes only one of them. Acetone may give the diabetic urine as well as the expired air the odor of apples or fruit. According to V. JAKSCH, acetone is a normal urinary con- stituent, though it may only occur in very small amounts (0.1 grm. THE URINE. 421 in the 24 hours). LE NOBEL claims that it only appears in healthy urine after taking alcohol or after food rich in proteids. Acetone as well as aceto-acetic acid seems to be a decomposition product of the albumins, and acetonuria may be caused by food very rich in proteids. In regard to the occurrence of these bodies under diseased con- ditions, a great many observations have been made, especially by v. JAKSCH, KAULICH, CANTANI, DEICHMULLER, FRERICHS, PEN- ZOLDT, LE NOBEL, SEIFERT, GERHARDT, and others. Acetonuria occurs in certain cases of diabetes, and especially in such patho- logical processes as are accompanied by an increased destruction of the tissues. It also occurs in fevers, in cachectic diseases, some- times in cancer of the organs of digestion, also in inanition and in psychosis. Acetonuria occurs especially often in children. Aceto-acetic acid never occurs in the urine as a physiological constituent, but appears under the same circumstances as acetone. It occurs frequently in children, in high fevers, acute exanthema, etc. Acetone, dimethyl ketone, C3H60 or CO.(OH3)2, is a thin water- olear liquid boiling at 56.5° C. and with a pleasant odor of fruit. It is lighter than water, with which it mixes in all proportions, also with alcohol and ether. The most important reactions for acetone are the following : LIEBEN'S lodoform Test. When a watery solution of acetone is treated with alkali and then with some iodine-potassium-iodide solution and gently warmed a yellow precipitate of iodoform is formed, which is known by its odor and by the appearance of the crystals (six-sided plates or stars) under the microscope. This reaction is very delicate, but it is not characteristic of acetone. GUNNING'S modification of the iodoform test consists in using an alcoholic solution of iodine and ammonia instead of the iodine dis- solved in potassium iodide and alkali hydrate. In this case, besides iodoform, a black precipitate of iodide of nitrogen is formed, but this gradually disappears on standing, leaving the iodoform visible. This modification has the advantage that it does not give any iodo- form with alcohol. On the other hand, it is not quite so delicate, but still it detects 0.01 milligramme acetone in 1 cc. REYNOLD'S mercuric-oxide test is based on the power of acetone 422 PHYSIOLOGICAL CHEMISTRY. to dissolve freshly-precipitated HgO. A mercuric-chloride solution is precipitated by alcoholic caustic potash. To this add the liquid to be tested for acetone, shake well and filter. In the presence of acetone the filtrate contains mercury, which may be detected by ammonium sulphide. This test has about the same delicacy as GUNNING'S test. LEGAI/S Sodium-nitroprusside Test. If an acetone solution is treated with a few drops of a freshly-prepared sodium-nitroprusside solution and then with caustic-potash or soda solution, the liquid is colored ruby-red. Creatinin gives the same color ; but if we sat- urate with acetic acid, the color becomes carmine or purplish red in the presence of acetone, but yellow and then gradually green and blue in the presence of creatinin. If we use ammonia instead of the caustic alkali (LE NOBEL), the reaction takes place with acetone but not with creatinin. LEGAI/S test indicates even 0.1 milligrm. acetone. PENZOLDT'S indigo test depends on the fact that orthonitroben- zaldehyde in alkaline solution with acetone yields indigo. A warm saturated and then cooled solution of the aldehyde is treated with the liquid to be tested for acetone and then with caustic soda. In the presence of acetone the liquid first becomes yellow, then green, and lastly indigo separates; and this may be dissolved with a blue color by shaking with chloroform. 1.6 milligrms. acetone can be detected by this test. Aceto-acetic Acid, or DIACETIC ACID, 04H603 or C2H3O.CH2. COOH. This acid is a colorless, strongly-acid liquid which mixes with water, alcohol, and ether in all proportions. On heating to boiling with water, and especially with acids, this acid decomposes into carbon dioxide and acetone, and therefore gives the above- mentioned reactions for acetone. It differs from acetone in that it gives a violet-red or brownish-red color with a dilute ferric-chloride solution. This color decreases even at the ordinary temperature within 24 hours, and more quickly on boiling. It differs in this from phenol, salicylic acid, acetic acid, or sulphocyanides. Detection of Acetone and Aceto-acetic Acid in the urine. Before testing for aceto-acetic acid test for acetone, and as this acid gradu- ally decomposes on allowing the urine to stand, the urine must be as fresh as possible. In the presence of aceto-acetic acid the urine THE URINE. 423 gives the so-called GERHARDT'S reaction, showing a wine-red color on the addition of a dilute, not too acid, ferric-chloride solution. Treat 10-50 c. c. of the urine with ferric chloride as long as it gives a precipitate, filter the precipitate of ferric phosphate, and add some more ferric chloride to the filtrate. In the presence of the acid a claret-red color is produced. After this heat a second, similar portion of the urine to boiling until faint acid reaction, and repeat the test on cooling, which should now give negative results. A third portion of urine is acidified with sulphuric acid and shaken with ether (which takes up the acid). Now shake the removed ether with a very dilute watery solution of ferric chloride, and the watery layer becomes violet-red or claret-red. The color disappears on warming. In the absence of aceto-acetic acid the acetone may be tested for directly. This may be done directly on the urine by LEGAI/S and PENZOLDT'S tests. These tests, which are only approximate tests, are only of value when the urine contains a considerable amount of acetone. For a more accurate test we distil at least 250 c. c. of the urine faintly acidified with sulphuric acid, care being taken to have a good condensation. Most of the acetone is contained in the first 10-20 c. c. of the distillate. This distillate is tested for acetone by the above tests. In testing for acetone in the simultaneous pres- ence of aceto-acetic acid, first make the urine faintly alkaline, and shake it carefully with ether free from alcohol and acetone in a separatory funnel. The removed ether is then shaken with water, which takes up the actone, and then the watery liquid is tested. • /?-Oxybutyric Acid, C4H803 or CH3.CH(OH).CH2.COOH. The appearance of this acid in the urine was first positively shown by MINKOWSKI, KiJLZ and STADELMANIST. It occurs especially in all difficult cases of diabetes, but it has also been observed in scarlet fever and in measles, in scurvy, and in diseases of the brain. /?-oxybutyric acid is accompanied by aceto-acetic acid in the urine. y#-oxybutyric acid forms an odorless syrup which mixes readily with water, alcohol, and ether. This acid is optically active and indeed laevo-gyrate, and it therefore interferes with the estimation of sugar in the urine by means of polarization. It is not precip- itated either by lead acetate or by ammoniacal basic lead acetate. On boiling with water, especially in the presence of a mineral acid, this acid decomposes into ^-CROTONIC ACID, which melts at 71°-72° C., and water: CH3.CH(OH).CH2.COOH =H20 + CH3.CH: 424 PHYSIOLOGICAL CHEMISTRY. CH.COOH. It yields acetone on oxidation with a chromate mix- ture. Detection of fi-Oxybutyric Acid in the urine. If a urine is still Jsevo-gyrate after fermentation with yeast, the presence of oxybu- tyric acid is probable. A further test may be made, according to KULZ, by evaporating the fermented urine to a syrup, and, after the addition of an equal volume of concentrated sulphuric acid, distil- ling directly without cooling. #-crotonic acid is produced which is distilled, and after strongly cooling the distillate is collected in a glass; crystals, which melt at -f 72° C., separate. If no crystals ' are obtained, then shake the distillate with ether, and test the melting-point with the residue, which has been washed with the water obtained after evaporating the ether. According to MIN- KOWSKI, the acid may be isolated as a silver-salt (see SCHMIEDE- BERG'S Archiv, 18, 35, or FRESENTUS' Zeitschrift, 24, 153). EHRLICH'S Urine Test. Mix 250 c. c. of a solution which contains 50 c. c. HC1 and 1 grm. sulphanilic acid in one litre with 5 c. c. of a \% solution of sodium nitrite (which produces very little of the active body, sulphodiazoben- zol). In performing this test treat the urine with an equal volume of this mixture, and then supersaturate with ammonia. Normal urine will become yellow thereby, or orange after the addition of ammonia (aromatic oxyacids may sometimes give after a certain time red azo bodies which color the upper layer of phosphate sediment). In pathological urines we sometimes have (and this is the characteristic diazo reaction) a primary yellow coloration, with a very marked secondary red coloration on the addition of ammonia, and the froth is also tinged with red. The upper layer of the sediment becomes green- ish. The body which gives this reaction is unknown, but it occurs especially in the urine of typhus patients (EHRLICH). Views are divided in regard to the significance of this reaction (EHRLICH, PENZOLDT, PETRI, ESCHERIK). FAT in the urine. The elimination of a urine which in appearance and richness in fat resembles chyle is called chyluria,. It contains habitually al- bumin, and often fibrin. Chyluria occurs mostly 'in the inhabitants of the tropics. Lipuria, or the elimination of fat with the urine, may appear in ap- parently healthy persons, sometimes with and sometimes without albumiuuria, in pregnancy, and also in certain diseases, as in diabetes, poisoning with phos- phorus, and fatty degeneration of the kidneys. Fat is usually detected by the microscope. It may also be dissolved with ether, and it may always be detected by evaporating the urine to dryness and extracting the residue with ether. CHOLESTERIN is also sometimes found in the urine in chyluria and in a few other cases. LEUCIN AND TYROSIN . These bodies are found in the urine, especially in acute yellow atrophy of the liver, in acute phosphorus- poisoning, and in difficult cases of typhus and smallpox. Detection of leucin and tyrosin. Tyrosin occurring as sediment may be identified by means of the microscope ; but if a positive proof is desired, a jrecrystallization of the same from ammonia or ammoniacal alcohol is necessary. To detect both these bodies when they occur in solution in the urine, pro- THE URINE. 425 ceed in the following manner : The urine free from albumin is precipitated by basic lead acetate, the lead removed from the filtrate by H8S, and concen- trated as much as possible. The residue is extracted with a small quantity of absolute alcohol to remove the urea. The residue is then boiled with faintly- ammoniacal alcohol, filtered, the filtrate evaporated to a small volume and allowed to crystallize. If no tyrosin crystals are obtained then dilute with water, precipitate again with lead acetate, and proceed as before. If tyrosin crystals now separate, they are filtered and the filtrate still further concen- trated to obtain the leucin crystals. Cystin, (C3H6NS02)2. This body is, according to BAUMANK, to be considered as disulphideH^>C<^^>C<^H of the previously-mentioned cystein, C3H7NS02 (page 393). This last substance, containing sulphur, is considered by BAUMANN as pyro- tartaric acid, whose ketone-oxygen atom is replaced by both the monatomic groups NH2 and SH or ^i|>S— • E.s> P f?i •r t& t SB aw-" 1 s'5 O e^ •S"? §•'2 r-B Is g-o < CD II b c o> II 2.5" ii s ii! •o o ° 111 p B a; o p 5* §.§ HI P 2. -8 pg tV B &,» PVS K «*• 00 2" 51 II 5g P* £-< ss- S5 1^ ptS B 3 » ^ l« B P The powder gives the murexid test The powder when treated with KHO gives S ere P B o o p- CHAPTER XV. THE EXCHANGE OF MATERIAL » WITH VARIOUS FOODS AND THE DEMAND FOR FOOD IN MAN. THE conversion of chemical tension into living energy, which characterizes animal life, leads, as previously stated in Chapter I, to the formation of the relatively simple compounds, carbon dioxide, urea, etc., which leave the organism and which moreover, being very poor in chemical tension, are for this reason of no or very little value for the body. It is therefore absolutely necessary for the continuance of life and the normal course of the functions of the body, that the organism and its different tissues should be supplied with new material to replace that which has been exhausted. This is accomplished by means of food. Those bodies are de- signated as food which have no injurious action upon the organism and which replace those constituents of the body that have been consumed in the exchange of material or that can prevent or diminish the consumption of such constituents. . Among the numerous dissimilar substances which man and animals take with the food all cannot be equally necessary or have the same value. Some perhaps are unnecessary, while others may be indispensable. We have learned by direct observation and a wide experience that besides the oxygen, which is necessary for oxidation, the essential foods for animals in general, and for man especially, are water, mine-mi bodies, proteid bodies, carbohydrates, and fats. It is also apparent that the various groups of food-stuffs neces- 1 The translator will use in the following pages for the German word " Stoffwechsel" Dr. Burdon-Sanderson's (Syllabus of Lectures, 1879) transla- tion, exchange of material, and at the same time the more general term "metabolism." 435 436 PHYSIOLOGICAL CHEMISTRY. Fary for the tissues and organs must be of different importance; thus, for instance, water and the mineral bodies have another task than the organic foods, and these again must vary in importance among themselves. The knowledge of the action of various nutri- tive bodies on the exchange of material from a qualitative as well as a quantitative point of view must be of fundamental importance in determining the value of different nutritive substances relative to the demands of the body for food under various conditions and also in deciding many other questions, for instance the proper nutrition for an individual in health and in disease. Such knowledge can only be attained by a series of systematic and thorough observations, in which the quantity of nutritive material, relative to the weight of the body, taken and absorbed in a given time is compared with the quantity of final products of the exchange of material which leave the organism at the same time. Researches of this kind have been made by several investigators, but above all should be mentioned those made by BISCHOFF and Von, by PETTENKOFEE and VOIT, and by VOIT and his school. It is absolutely necessary in researches on the exchange of material to be able to collect, analyze, and quantitatively estimate the excretions of the organism so that they may be compared with the quantity and composition of the nutritive bodies taken up. In the first place, one must know what the habitual excretions of the body are and in what way these bodies leave the organism. One must also have trustworthy methods for the quantitative estimation of the same. The organism may, under physiological conditions, be exposed to accidental or periodic losses of valuable material — such losses as only occur in certain individuals, or in the same individual only at a certain period; for instance, the secretion of milk, the produc- tion of pus, the ejection of semen, or menstrul blood. It is therefore apparent that these losses can only be the subject of investigation and estimation in special cases. The regular and constant excretions of the organism are of the very greatest importance in the study of the exchange of material. To these belong, in the first place, the true final products of the exchange — CARBON DIOXIDE, UREA (uric acid, hippuric acid, creatinin, and other urinary constituents), and a part of the WATER, EXCHANGE OF MATERIAL. 437 The remainder of the water, the mineral bodies, and those secretions or tissue-constituents — MUCUS, DIGESTIVE FLUIDS, SEBUM, SWEAT, and EPIDERMIS FORMATIONS — which are either poured into the intestinal tract, or secreted from the surface of the body, or broken off and thereby lost for the body, also belong to the constant excreta. The remains of food, sometimes indigestible, sometimes digest- ible but not acted upon, contained in the faeces, which vary in quantity and composition with the nature of the food, also belong to the excreta of the organism. Even though these remains, which are never absorbed and therefore are never constituents of the animal fluids or tissues, cannot be considered as excreta of the body in a strict sense ; still their quantitative estimation is absolutely necessary in experiments on the exchange of material. The determination of the constant loss is in some cases accom- panied with the greatest difficulties. The loss from the detached epidermis, from the secretion of the sebaceous glands, etc., cannot be determined with exactness without difficulty, and therefore — as they do not occasion any mentionable loss because of their small quantity — they need not be considered in quantitative experiments on the exchange of material. This also applies to the constituents of the mucus, bile, pancreatic and intestinal juices, etc., occurring in the contents of the intestines and which, leaving the body with the faeces, cannot be separated from, the other contents of the intestines and therefore cannot be quantitatively determined sepa- rately. The uncertainty which, because of the intimated difficulties, attaches itself to the results of the experiments is very small as compared to the variation which is caused by different individuali- ties, different modes of living, different foods, etc. No general but only approximate value can therefore be given for the constant excretions of the human body. The following figures represent the quantity of excreta for 24 hours, with a mixed diet, of a grown man weighing 60-70 kilos. The numbers are compiled from the results of different investi- gators. Grammes. Water 2500-3500 Salts (with the urine) 20-30 Carbon dioxide 750-900 Urea 20-40 Other nitrogenized urinary constituents 2-5 Solids in the excrements . . 30-50 438 PHYSIOLOGICAL CHEMISTRY. These total excreta are approximately divided among the various excretions in the following way — but still it must not be forgotten that this division may vary to a great extent under various external circumstances: by RESPIRATION about 32$, by the EVAPORATION FROM THE SKIN 17$, with the URINE 46-47$, and with the EXCRE- MENTS 5-9$. The elimination by the skin and lungs, which is sometimes differentiated by the name " PERSPIRATIO INSENSIBILIS" from the visible elimination by the kidneys and intestine, is on an average about 50$ of the total elimination. This proportion, only quoted relatively, is subject to considerable variation, because of the great difference, in the loss of water through the skin and kidneys under different circumstances. About 90$ of the water in carnivora is excreted through the kidneys. In herbivora the excrements, which are 30-50$ of the total excreta, may indeed eliminate 60$ of the water. In man only a smaller fraction of the water (about 5$) is eliminated with the faeces, and the great mass of the water is divided between the kid- neys, lungs, and skin. The riitrogenized constituents of the excretions consist chiefly of urea, or uric acid in certain animals, and the other nitrogenized urinary constituents. A disproportionally large part of the nitrogen leaves the body with the urine; and as the nitrogenized constitu- ents of this excretion are final products of the transformation or metabolism of proteids in the organism, the quantity of proteids transformed in the body may be easily calculated by multiply- ing the quantity of nitrogen in the urine by the coefficient 6.25 (Vr — 6.25), if we admit that the proteids contain in round numbers 16$ nitrogen. Still another question is whether the. nitrogen leaves the body only with the urine or by other channels. This last is habitually the case. The discharges from the intestine always contain some nitrogen which has a twofold origin. A part of this nitrogen de- pends upon undigested or non-absorbed remnants of food, and an- other part on the non-absorbed remains of digestive secretions — bile, pancreatic juice, intestinal mucus — and of epithelium-cells of the mucous membrane. It follows that a part of the nitrogen of faeces has this last-mentioned origin from the fact that discharges from the intestine occurs also in complete inanition. EXCHANGE OF MATERIAL. 439 If the question to be decided is, how much of the nitrogenized bodies is absorbed in certain modes of nutrition or after taking a certain quantity of food, then naturally the quantity of nitrogen originating from the food and leaving the body with the excre- ments must be subtracted from the total quantity of nitrogen taken up with the food. To obtain the quantity of nitrogen leaving the body with the excrements it is necessary to subtract from the total quantity of nitrogen of the excrements the quantity of nitrogen coming from the digestive tract itself and its secretions, and the amount of this last must be known. It is obvious that exact results which answer for all times can- not be given for that part of the nitrogen which has its origin in the digestive canal and fluids. It may not only vary in different individuals, but also in the same individual after more or less active secretion and absorption. In the attempts made to determine this part of the nitrogen of the excrements it has been found that in man, on non-nitrogenized or nearly nitrogen-free food, it amounts in round numbers to about 1 grm. per 24 hours (RiEDER; RUBNER). During starvation, in which a smaller quantity of digestive secre- tions is eliminated, it is less. MULLER found in his observations on the faster CETTI that only 0.2 grm. nitrogen was derived from the intestinal canal. Nitrogen also leaves the body through the horn formation. The quantity which is lost in this manner is, though it cannot be ex- actly determined, insignificant. Man loses only about 0.3 grm. nitrogen daily by means of the hair and nails (MOLESCHOTT) and about 0.3-0.5 grm. by the scaling-off of the skin. The quantity of nitrogen which leaves the body under ordinary circumstances by the perspiration must be so small that, like the loss by the horny structure, it need not be considered in experiments on the exchange of material. The elimination of nitrogen by the perspiration need only be considered in cases of profuse sweating. The view was formerly held that in man and carnivora an elim- ination of gaseous nitrogen took place through the skin and lungs, and because of this, on comparing the nitrogen of the food with that of the urine and faeces, a nitrogen deficit occurred in the vis- ible elimination. This question has been the subject of much discussion and of 440 PHYSIOLOGICAL CHEMISTRY. numerous investigations. The conclusion has been drawn from the researches of REGNAULT and REISET on respiration that also an exhalation of nitrogen takes place. SEEGEN and NOWAK espe- cially have recently endeavored to prove the correctness of this con- clusion. Such an experiment is, however, accompanied with so many difficulties, and there are so many sources of error, that it can scarcely be considered as conclusive. In fact, PETTENKOFER and VOIT have demonstrated the existence of errors in the experiments of SEEGEN and NOWAK. On the other hand, PFLUGER and LEO have found no appreciable exhalation of nitrogen in rabbits. Also many investigators, especially VOIT, PETTENKOFER and VOIT and RANKE, have shown by experiments on man that with the proper amount and quality of food we can bring the body into NITROGE- NOUS EQUILIBRIUM, in which the quantity of nitrogen voided with the urine and faeces is equal to the quantity contained in the food. The experiments made by GRUBER in VOIT'S institute seem to be especially conclusive on this point. GRUBER fed a dog seventeen days on meat which in all contained 368.53 grms. nitrogen, and he found in the same time 368.28 grms. nitrogen in the urine and faeces. From this and other experiments we may conclude with VOIT that a deficit of nitrogen does not exist; or if we consider the above-mentioned very small loss of nitrogen through the horny structure, etc., it is so insignificant that in experiments upon the exchange of material it need not be considered. In investigations on the metabolism of proteids in the body, ordinarily, it is only necessary to consider the nitrogen of the urine and faeces, but it must be remarked that the nitrogen of the urine is a measure of the extent of the metabolism of the proteids in the body, while the nitrogen of the faeces (after deducting somewhat less than 1 grm. on mixed diet) is a measure of the non-absorbed part of the nitrogen of the food. In the oxidation of the proteids in the organism their sulphur is oxidized into sulphuric acid, and on this depends the fact that the elimination of sulphuric acid by the urine, which in man is only to a small extent derived from the sulphates of the food, makes nearly equal variations as the elimination of nitrogen by the urine. If we consider the amount of nitrogen and sulphur in the proteids as 16$ and 1$ respectively, then the proportion between the nitrogen EXCHANGE OF MATERIAL. 441 of the proteids and the sulphuric acid, H2S00 produced by their burning, is in the ratio 5.2:1, or about the same as in the urine (see page 382). The determination of the quantity of sulphuric acid eliminated with the urine gives us an important means of con- trolling the extent of the transformation of proteids, and such a control is especially important in cases in which we wish to study the action of certain nitrogenized non-albuminous bodies on the metabolism of proteids. A determination of the nitrogen alone is not in such cases sufficient. If it is found, on comparing the nitrogen of the food with that of the urine and faeces, that there is an excess of the first, this means that the body has increased its stock of nitrogenized substances — proteids. If, on the contrary, the urine and faeces contain more nitrogen than the food taken at the same time, this denotes that the body is giving up part of its nitrogen — that is, a part of its own proteids. We can from the quantity of nitrogen, as above stated, calculate the corresponding quantity of proteids by multiplying by 6.25. Usually, according to VOIT'S proposition, the nitrogen of the urine is not calculated as decomposed proteids, but as decom- posed muscle-substance. Flesh contains on an average about 3.4$ nitrogen, and each gramme of nitrogen of the urine corresponds in round numbers to about 30 grms. flesh. A disproportionally large part of the carbon leaves the body as carbon dioxide, which escapes chiefly through the lungs and skin. The remainder of the carbon is eliminated under the form of organic combinations by the urine and faeces, in which the quantity of carbon can be determined by elementary analysis. The amount of gaseous carbon dioxide eliminated is determined by means of PETTENKOFER'S respiration apparatus, which is described in special works. By multiplying the quantity of carbon dioxide found by 0.273 we obtain the quantity of carbon eliminated as C02. If we compare the total quantity of carbon. eliminated in various ways with the carbon contained in the food, we obtain some idea as to the transformation of the carbon compounds. If the quantity of carbon in the food is greater than in the excretion, then the excess is deposited; while if the reverse be the case, it shows a correspond- ing loss of bodily substance. The nature of the substances here deposited or lost, whether 442 PHYSIOLOGICAL CHEMISTRY. they consist of proteids, fats, or carbohydrates, is learned from the total quantity of nitrogen of the excretions. The corresponding quantity of proteids may be calculated from the quantity of nitro- gen, and as the average quantity of carbon in the proteids is known, the quantity of carbon which corresponds to the decomposed pro- teid may be easily ascertained. If the quantity of carbon found is smaller than the quantity of the total carbon in the excretions, it is then obvious that some other nitrogen-free substance has been con- sumed besides the proteids. If the amount of carbon in the pro- teids is considered in round numbers as 54$, then the relation between carbon (54) and nitrogen (16) is as 3.4:1. Multiply the total quantity of nitrogen eliminated by 3.4, and the excess of carbon in the eliminations over the product found represents the carbon of the transformed non-nitrogenized compounds. For instance, in the case of a person experimented upon, 10 grms. nitro- gen and 200 grms. carbon were eliminated in the course of 24 hours; then these 62.5 grms. proteid correspond to 34 grms. carbon, and the difference 200 — (3.4x10) = 166, which represents the quantity of carbon in the decomposed non-nitrogenized compounds. If we start from the simplest case, starvation, where the body lives at the expense of its own substance, then, since the quantity of carbohy- drates as compared to the fats of the body is extremely small, in such cases in order to avoid mistakes the assumption must be made that the person experimented upon has only taken fat and proteids. As animal fat contains on an average 76. 5$ carbon, the quantity of transformed fat may be calculated by multiplying the carbon by -i r\r\ j— — = 1.3. In the case of the above example the person 7o.o experimented upon would have used 62.5 grms. proteids and 166 x 1.3 = 216 grms. fat of his own bodily weight in the course of the 24 hours. Starting from the balance of the nitrogen, we can calculate in the same way whether an excess of carbon in the food as compared with the quantity of carbon in the excretions is retained by the body as proteids or fat or as both. On the other hand, with an excess of carbon in the excretions we can calculate how much of the loss of the substance of the body is due to a consumption of the proteid or of fat or of both. EXCHANGE OF MATERIAL.. 443 The quantity of water and mineral bodies void eel with the urine and faeces can easily be determined. The quantity of water elimi- nated by the skin and lungs may be directly determined by means of PETTENROFER'S apparatus. The quantity of oxygen taken up is calculated as the difference between the weight of the individual before the experiment plus all the directly-determined substances taken in, and the final weight of the individual plus all his excreta. I. Exchange of Material in Starvation. In starvation the decomposition in the body continues uninter- ruptedly, though with decreased intensity; but as it takes place at the expense of the substance of the body, it can only continue for a limited time. When an animal has lost a certain fraction of the mass of the body death is the result. This fraction varies with the condi- tion of the body at the beginning of the starvation period. Fat ani- mals succumb when the weight of the body has sunk to J of the orig- inal weight. Otherwise, according to CHOSSAT, animals die as a rule when the weight of the body has sunk to f of the original weight. The period when death occurs from starvation not only varies with the different nutritive condition at the beginning of starvation, but also with the more or less active exchange of material. This is more active in small and young animals than in large and older ones, but different classes of animals show an unequal activity. Children succumb in starvation in three to five days after having lost J of their bodily mass. Grown persons, according to ordinary state- ments, can live for three weeks if they have water ; we also have statements of much longer periods of starvation. For instance, a person suffering from melancholia, who drank water but took no food, died after 41 days, and the Italian MERLATTI withstood a starvation period of 50 days, during which it is stated he only took water. - Dogs can live without food from four to eight weeks, birds five to twenty days, snakes more than half a year, and frogs more than a year. In starvation the weight of the body decreases. The loss of weight is greatest in the first few days, and then decreases rather uniformly. In small animals the absolute loss of weight per day is naturally smaller than in larger animals. The relative loss of weight, 444 PHYSIOLOGICAL CHEMISTRY. that is, the loss of weight of the unit of the weight of the body, namely 1 kilo, is, on the contrary, greater in small animals than in larger ones. The reason for this is that the smaller animals have a greater surface of body in proportion to their mass than larger animals, and the greater loss of heat caused hereby must be replaced by a more active consumption of material (RUBBER). Exact observations for a long time are necessary for a thorough study of the exchange of material in starvation. As these have seldom been made on man our knowledge of the exchange of material in starvation has been gained by observations on animals, especially on carnivora. As the exchange of material in starvation takes place at the expense of the constituents of the body, it must take place in essentially the same way in both carnivora and herbivora. As the food of the herbivora is ordinarily richer in carbohydrates and non- nitrogenized nutritive bodies than that of the carnivora, so in star- vation the body of the herbivora becomes relatively richer in proteids. On this account the elimination of urea is increased in herbivora in the first part of the period of starvation. In carniv- ora the elimination of urea decreases, as a rule, immediately at the beginning of the starvation, and in the later periods only small amounts of urea are voided by herbivora as well as by carnivora. The extent of the metabolism of proteids and the elimination of urea, which is a measure for the same, does not show in carnivora any uniform decrease during the entire period of starvation. During the first few days the elimination of nitrogen is greatest and the quantity of the same depends essentially upon the amount of proteids in the organism and the nature of the food previously taken. The richer the body is in proteids from the food previously taken the greater is the metabolism of proteids, or, in other words, the elimination of nitrogen during the first days of starvation is greater. The rapidity with which the elimination of nitrogen decreases in the first days depends also, according to VOIT, upon the proteid condition of the body. It decreases more quickly, that is, the curve of the decrease is more sudden, the first days of starvation, as a rule, the richer the food was in proteids which was taken before starvation. This condition is apparent from the fol- lowing table. This table contains three different starvation experi- EXCHANGE OF MATERIAL. 445 ments made by VOIT on the same dog. This dog received 2500 grms. flesh daily before the first series of experiments, 1500 grms. flesh daily before the second series, and a mixed food relatively poor in nitrogen before the third series. TABLE I. Day of Starvation. 1 ................ 60.1 26.5 13.8 2 .............. 24.9 18.6 11.5 3 ............... 19.1 15.7 10.2 4 ........... .... 17.3 14.9 12.2 5 ................ 12.3 14.8 12.1 6 ................ 13.3 12.8 12.6 7 ................ 12.5 12.9 11.3 8.... ............ 10.1 12.1 10.7 Other conditions, such as varying amounts of fat in the bodyf have an influence on the rapidity with which the nitrogen is eliminated during the first days of starvation. After the first few days the elimination of nitrogen, as is seen in the above table, is more uniform, and as the starvation proceeds it decreases as a rule very slowly and uniformly. Cases also occur in which the elimina- tion of nitrogen becomes constant in these stages, and in which indeed the elimination of nitrogen increases towards the end. This so-called premortal increase always occurs as soon as the adipose tissue in the body has sunk to a certain point, and it also depends on the fact that as soon as the fat is consumed a corre- sponding increase in the decomposition of proteids is necessary for the generation of heat as well as of other forces. If fat occurs in the body besides proteids, it is also decomposed in starvation. Since fat has a diminishing influence on the de- struction of proteids (see further on), the elimination of nitrogen in starvation is less in fat than in lean individuals. For instance, only 9 grms. of urea were voided in twenty-four hours during the later stages of starvation by a well-nourished and fat person suffer- ing from disease of the brain, while J. MUNK found that 20-29 grms. urea were voided daily by CETTI, who had been poorly fed. Like the destruction of proteids during starvation, the decom- position of fat proceeds uninterruptedly. The decomposition of fat does not show so great and rapid a decrease in the first days of 446 PHYSIOLOGICAL CHEMISTRY. starvation as the destruction of proteids. PETTENKOFER and VOIT found, for instance, in a starving dog the following losses of proteids and fat from the body on different days of starvation: TABLE II. naTr Weight of Body Loss in Oxygen Dfty- in Kilos. Flesh. Fat, absorbed. 2 32.9 341 86 5 31.7 167 103 358 8 30.5 138 99 333 The consumption of fat on the second day, when the decom- position of proteids was considerable, was indeed less than in the following days. The conditions for the destruction of proteids in the animal body seem, as VOIT has suggested, to be different from those for the consumption of fat. The constant decrease in the consumption of the adipose tissue and proteids during starvation must also cause a decrease in the extent of the exchange of gas / for instance, a diminished taking up of oxygen and a diminished elimination of carbon dioxide. This is found to be true. In starvation experiments made on a cat, SCHMIDT found that the results for the carbon dioxide and oxygen fell in the course of eighteen days from 50.96 grms. carbon dioxide and 46.20 grms. oxygen on the first day of starvation to 22.26 grms. carbon dioxide and 22.12 grms. oxygen on the last day. Investigations on the extent of exchange of gas in human beings in the starving condition were made by LEHMANN and ZUNTZ on CETTI, who only partook of water for ten days. These investi- gators found that the absolute extent of exchange of gas during hunger decreases, but that when the oxygen consumed and the carbon dioxide eliminated were calculated on the unit of the weight of the body — 1 kilo — its amount quickly sinks to a minimum, but then remains unchanged or may perhaps rise during the course of the fast. They found a consumption of 4.65 c. c. oxygen per minute for 1 kilo during the third to sixth day and 4.73 c. c. during the ninth to eleventh day. It is also a well-known fact that the temperature of the body of starving animals remains tolerably con- stant, without showing a mentionable decrease, during the greater EXCHANGE OF MATERIAL. 447 part of the starvation period. The heat of the animal sinks only a few days before death, and at about 33° to 30° C. death results. If the carbon is burnt with oxygen into carbon dioxide, the carbon dioxide produced occupies the same volume as the oxygen OO consumed, and the quotient —^ is therefore 1. The same is true of the burning of the carbohydrates, which contain in themselves the necessary quantity of oxygen to oxidize the hydrogen, and only the quantity of oxygen required to oxidize the carbon is necessary to be taken up for the burning of the carbohydrates into C02 and H20. In the burning of fats and proteids this is different. In these cases an absorption of oxygen is necessary not only for the burning of the carbon but also for the hydrogen, and the volume of the carbon dioxide formed is therefore smaller than the oxygen PO consumed. The quotient — ~-^ must therefore in these cases be smaller than 1. The conditions are still more complicated for the proteids, because these bodies contain sulphur which is oxidised into sulphuric acid, and also because they are not completely burnt in the organism, but yield nitrogenized decomposition products which contain hydrogen and oxygen as well as carbon. From the above it follows that in man, on a mixed diet, the proportion between the inhaled oxygen and the expired carbon dioxide, or the so-called respiratory quotient, must be smaller than 1 . As a rule, it is 0.73-0.86 on a mixed diet. On feeding with an exclusively vegetable food rich in carbohydrates it is closer to 1 ; with a strictly meat diet it is lowest, about 0.7. In starvation, when the person or animal lives entirely upon his own body, it must be about the same as when fed entirely upon meat and fat. As the quotient for the burning of proteids is 0.81-0.75, and for the burning of fats 0.7, the respiratory quotient in starvation must be in the neighborhood of 0.7. In the above-mentioned starvation experiments made by C. SCHMIDT on a cat it was 0.765, while in his observations on CETTI it was still lower, or 0.68-0.65. Water passes uninterruptedly from the body in starvation even when none is given. If the amount of water in the tissues rich in proteids is considered as 70-80$, and the amount of proteids in the same 20$, then for each gramme of destroyed proteids about 448 PHYSIOLOGICAL CHEMISTS T. 4 grammes of water are set free. A special increase in the demand for water does not seem to occur in starving animals. The mineral substances leave the body uninterruptedly in starva- tion until death, and the influence of the destruction of tissues is plainly perceptible by their elimination. Because of the destruction of tissues rich in potassium, the proportion between potassium and sodium in the urine changes in starvation so that, contrary to the normal conditions, the potassium is eliminated in proportion- ally greater quantities. MUNK also observed in CETTI'S case a relative increase in the phosphoric acid and calcium in the urine during starvation, which was due to an increased exchange of bone- substance. The question as to the participation of the different organs in the loss of weight of the body during starvation is of special interest. To illustrate this question we will give below the results of CHOSSAT'S experiments on pigeons and those of VOIT on a male cat. The results are percentages of weight lost from the original weight of the organ. TABLE III. Pigeon (CHOSSAT). Male Cat (VoiT). Fat 93 per cent. 97 per cent. Spleen 71 67 Pancreas 64 17 Liver 52 54 Heart 45 3 Intestines 42 18 Muscles 42 31 Testicles 40 Skin 33 21 Kidneys 32 26 Lungs 22 ' 18 Bones 17 ' 14 Nervous system . . 2 3 The total quantity of blood, as well as the amount of solids con- tained therein, decreases, as PANUM has shown, in the same pro- portion as the weight of the body. The statements in regard to the loss of water by different organs is somewhat contradictory ; according to LUKJANOW, it seems that the various organs act some- what differently in this respect. The above-tabulated results cannot serve as a measure of the EXCHANGE OF MATERIAL. 449 exchange of material in the various organs during starvation. For instance, the nervous system only shows a small loss of weight as compared to the other organs, but from this it must not be con- cluded that the exchange of material in this system of organs is not active. The condition may be quite different ; for one organ may derive its nutriment during starvation from some other organ and exist at its expense. A positive conclusion cannot be drawn in regard to the activity of the exchange of material in an organ from the loss of weight of that organ in starvation. II. Exchange of Material with Inadequate Nutrition. The food may be quantitatively insufficient and the final result is absolute inanition. The food may also be qualitatively insuffi- cient, or as we say, inadequate. This occurs when any of the necessary nutritive bodies are absent in the food, while the others occur in sufficient or perhaps indeed in excessive amounts. Lack of water in the food. The quantity of water in the organism is greatest during foetal life, and then decreases with increasing age (v. BEZOLD). Naturally, the amount differs in various organs. Enamel, being almost free, contains only 2 p. m. water, the teeth about 100 p. m., the fatty tissues and bones 60-120-150 p. m. The cartilage with 540-740 p. m. is somewhat richer in water, while the muscles, blood, and glands with 750 to more than 800 p. m. are still richer. The quantity of water is even greater in the animal fluids (see preceding chapter), and the grown body contains in all about 600 p. m. water (BISCHOFF). If we bear in mind that two thirds of the animal organism consists of water ; that water is of the very greatest importance in the normal, phy- sical composition of the tissues ; moreover that all flow of juices, all exchange of substance, all supply of nutrition, all increase or destruction, and all discharge of the products of destruction are dependent upon the presence of water; besides this, that by its evaporation it is an important regulator of the temperature of the body, — we perceive that water must be necessary for life. If the loss of water be not replaced by fresh supplies sooner or later, the organism succumbs. Lack of mineral substances in the food. We are chiefly in- 450 PHYSIOLOGICAL CHEMISTRY. debted to Liebig for showing that the mineral substances are just as necessary for the normal composition of the tissues and organs, and for the normal course of the processes of life, as the organic constituents of the body. The importance of the mineral constit- uents is evident from the fact that there is no animal tissue or animal fluid which does not contain mineral substance, and also from the fact that certain tissues or elements of tissues contain habitually certain mineral substances and not others, which ex- plains the unequal division of the potassium and sodium compounds in the tissues and fluids. With the exception of the skeleton, which contains about 220 p. m. mineral bodies (VoLKMANtf), the animal fluids or tissues are poor in inorganic constituents, and the quan- tity of such only amounts, as a rule, to about 10 p. m. Of the total quantity of mineral substances in the organism, the greatest part occurs in the skeleton, 830 p. m., and the next greatest in the mus- cles, about 100 p. m. (VoLKMAtftf). The mineral bodies seem to be partly dissolved in the fluids and partly combined with organic substances. In accordance with this the organism persistently retains, with food poor in salts, a part of the mineral substances, also such as are soluble, as the chlorides. On the burning of the organic substances the mineral bodies com- bined therewith are set free and are eliminated. It is also ad- mitted that they in part combine with the new products of the burning, and also that they in part are attached to organic nutri- tive bodies absorbed from the intestinal canal which are poor in salts, or nearly salt-free, and are thus retained (VoiT, FORSTER). If this statement be correct, it is possible that a constant supply of mineral substances with the food is not absolutely necessary, and that the amount of inorganic bodies which must be administered is insignificant. The question whether this be so or not has not, especially in man, been sufficiently investigated ; but generally we consider the need of mineral substances by man as very small. It •may, however, be assumed that man usually takes with his food a ^considerable excess of mineral substances. Investigations on animals in regard to the action of an insufficient supply of mineral substances with the food have been made by sev- eral investigators, especially FORSTER. He observed, on experiment- ing on dogs and pigeons, with food as poor as possible in mineral sub- EXCHANGE OF MATERIAL. 451 stances, a very suggestive disturbance of the functions of the organs, especially the muscles and the nervous system, and death resulted after a time, indeed earlier than in complete starvation. In opposi- tion to these observations BUNGE has suggested that the early death in these cases was not caused by the lack of mineral salts, but more likely by the lack of bases necessary to neutralize the sulphuric acid formed in the burning of the proteids in the organism, which must be then taken from the tissues. In accordance with this view, BUXGE and LUNIN" also found on experimenting on mice that ani- mals which received nearly ash-free food with the addition of sodium carbonate were kept alive twice as long as animals which had the same food without the addition of sodium carbonate. Spe- cial experiments also show that the carbonate cannot be replaced by an equivalent amount of sodium chloride, and that to all appear- ances it acts by combining with the acids formed in the body. The addition of alkali carbonate to the otherwise nearly ash-free food may indeed delay death, but cannot prevent it, and even in the presence of the necessary amount of bases death results for lack of mineral substances in the food. In the above series of experiments made by BUNGE the food of the animal consisted of casein, milk-fat, and cane-sugar. While milk alone was an adequate and sufficient food for the animal, BUNGE found that the animal could not be kept alive longer by food consisting of the above constituents of milt and cane-sugar, with the addition of all the mineral substances of milk, than with the food mentioned in the above experiments with the addition of alkali carbonate. The question whether this result is to be ex- plained by the fact that the mineral bodies of milk are chemically combined with the organic constituents of the same and can be assimilated only in such combinations, or whether it depends on other conditions, BUNGE leaves undecided. These observations, however, show how difficult it is to draw positive conclusions from experiments made thus far with food poor in salts. Further investi- gations on this subject seem to be necessary. With an insufficient supply of chlorides with the food the elimi- nation of chlorine by the urine decreases constantly, and at last it may stop entirely while the tissues still persistenly retain the chlo- rides. These last are, at least in part, combined in the body with 452 PHYSIOLOGICAL CHEMISTRY. the organic substances which retain them. The great importance of such a retention of chlorides *by the tissues is apparent if we bear in mind that the NaCl is not only a solvent for certain albuminous bodies, or a material for the elaboration of the gastric juice, but that it is also of the greatest importance as a so-called indifferent salt for the preservation of the normal consistency and the physio- logical imbibition relation of the tissues. If there be a lack of sodium as compared to potassium, also if there be an excess of potassium compounds in any other form than KC1, the potassium combinations are replaced in the organism by NaCl, so that new potassium and sodium compounds are produced which are voided with the urine. The organism becomes poorer in NaCl, which therefore must be taken in greater amounts from the outside (BUNGE). This occurs habitually in herbivora, and in man with vegetable food rich in potash. For human beings, and especially for the poorer classes of people who live chiefly on pota- toes and foods rich in potash, common salt is, under these circum- stances, not only a condiment, but a necessary addition to the food (BUKGE). Lack of alkali carbonates or bases in the food. The chemical processes in the organism are dependent upon the presence of alkaline-reacting tissue-fluids, whose alkaline reaction is due to alkali carbonates. The alkali carbonates are also of great impor- tance not only as a solvent for certain albuminous bodies and as constituents of certain secretions, as of the pancreatic and intes- tinal juices, but they are also a means of transportation of the carbon dioxide in the blood. It is therefore easy to understand that a decrease below a certain point in the quantity of alkali car- bonate must endanger life. Such a decrease not only occurs with lack of bases in the food which accelerates death by a relatively too great production of acids by the burning of the proteids (see above: BUNGE and LUKIN), but it also occurs when an animal is given dilute mineral acids for a certain time. In herbivora the fixed alkalies of the tissues combine with the mineral acids, and the animal succumbs after a time. In carnivora the bases of the tis- sues are obstinately retained; the mineral acids unite with the ammonia produced by the decomposition of the proteids or their EXCHANGE OF MATERIAL. 453 splitting products, and carnivora can therefore be kept alive for a longer time. Lack of earthy phosphates. With the exception of the impor- tance of the alkaline earths as carbonates and principally as phos- phates in the physical composition of certain structures, such as the bones and teeth, their physiological importance is nearly un- known. The action which an insufficient supply of alkali-earths with the food causes is connected with the interesting question as to the eifect of this lack upon the bony structure. This action, as well as the various results obtained by experiments on young and old animals, has already been spoken of in Chap. VIII, to which we refer the reader. Lack of iron. As iron is an integral constituent of haemoglobin, indispensable for the introduction of oxygen, so also it is an indis- pensable constituent of the food. In iron starvation iron is con- tinually eliminated, even though in diminished amounts (HAM- BUBGER, DIETL, v. HossLiN). From the observations of v. HOSSLIN on dogs it also seems that an inadequate supply of iron with the food causes an insufficient formation of haemoglobin. A special result of the lack of iron is chlorosis, which the physician has often to contend with and whose origin is not really a lack of iron in the food, but more likely an incomplete absorption of the foods containing iron (BuNGE). The iron-salts seem not to be ab- sorbed at all in the intestinal canal, or only to a very small extent, so that it is questionable whether their absorption has any men- tionable importance. It seems more probable that the absorption of iron from the food takes place in the form of protein bodies (nucleoalbumin) containing iron (BUNGE); and the importance of the iron-salts in preventing the lack of haemoglobin consists chiefly, according to BTOGE, in that these salts counteract the decomposi- tion in the intestines of the protein bodies containing iron, which split off iron as iron sulphide. In the absence of proteid bodies in the food the organism must nourish itself by its own proteid substances, and on such nutrition it must earlier or later succumb. By the exclusive administration of fat and carbohydrates the consumption of proteids in these cases is reduced, for by an exclusive fat and carbohydrate diet the metabolism of proteids may indeed be smaller than in complete 454 PHYSIOLOGICAL CHEMISTRY. starvation (HIRSOHFELD). In conformity with this the animal may be kept alive longer by food containing only non-nitrogenized bodies than in complete starvation. The absence of fats and carbohydrates in the food affect car- nivora and herbivora somewhat differently. It is unknown whether carnivora can be kept alive for any length of time by food en- tirely free from fat and carbohydrates. But it has been positively demonstrated that they cannot only be kept alive by feeding entirely with meat freed as much as possible from visible fat, but that flesh, and perhaps also fat, is deposited. Men or herbivora, on the con- trary, cannot live for any length of time on such food. On one side they lose the property of digesting and assimilating the neces- sarily large amounts of meat, and on the other a distaste for large quantities of meat or proteids soon appears. III. Exchange of Material with Various Foods. For the carnivora, as above stated, meat as poor as possible in fat may be a complete and sufficient food. As the proteids more- over take a special place among the organic nutritive bodies by the quantity of nitrogen they contain, it is proper that we first describe the exchange of material with an entirely meat diet. Exchange of material with food rich in proteids, or feeding only with meat as poor in fat as possible. By an increased supply of proteids the metabolization of pro- teids and the elimination of nitrogen is increased and indeed in proportion to the supply of proteids. If a certain quantity of meat is given as food daily to carnivora and the ration of meat is suddenly increased, an increased metab- olism of proteids or an increase in the quantity of nitrogen elim- inated is the result. If we feed the animal daily for a certain time with larger quantities of the same meat, we find that a part of the proteids accumulates in the body, but this part decreases from day to day, while there is a corresponding daily increase in the elimina- tion of nitrogen. In this way a nitrogenous equilibrium is estab- lished, that is, the total quantity of nitrogen eliminated is equal to the quantity of nitrogen in the absorbed proteids or meat. If, on the contrary, an animal which is in nitrogenous equilibrium having EXCHANGE OF MATERIAL. 455 been fed on large quantities of meat is suddenly fed with a small quantity of meat per day, then the animal gives up its own bodily proteids, the amount decreasing from day to day. The elimination of nitrogen and the metabolism of proteids decrease constantly, and the animal may in this case also pass into nitrogenous equilibrium or nearly into this condition. These conditions are illustrated by the following table (Vorr) : TABLE IV. Grms. of Flesh in the Food per Day. Before the Test. During the Test' 1 500 1500 2. . ... 1500 1000 Grms. of Flesh metabolized in Body per Day. '1 2 3 ~4~~ 5 6 T" 1222 1310 1390 1410 1440 1450 1500 1153 1086 1088 1080 1027 In the first case (1) the metabolism of flesh before the beginning of the actual experiment on feeding with 500 grms. meat was 447 grms., and it increased considerably on the first day of the experi- ment, after feeding on 1500 grms. meat. In the second case (2),. in which the animal was previously in nitrogenous equilibrium with 1500 grms. meat, the metabolism of flesh on the first day of the experiment, with only 1000 grms. meat, decreased considerably, and on the fifth day a nearly nitrogenous equilibrium was obtained. During this time the animal gave up daily some of its own proteids. Between that point below which the animal loses from its own weight and the maximum, which seems to be dependent upon the digestive and assimilative capacity of the intestinal canal, car- nivora may be kept in a nitrogenous equilibrium with a varying quantity of proteids in the food. The supply of proteids, as well as the condition of the body, affects the extent of the proteid metabolism. A body which has become rich in proteids by a previous abundant meat diet must, to prevent a loss of proteids, take up more proteid with the food than a body poor in proteids. The quantity of fat in the body also has a great influence. As the fat of the body lowers the disintegration 456 PHYSIOLOGICAL CHEMISTRY. of proteids in starvation, so also it diminishes the disintegration of the proteids taken with the food. PETTENKOFER and VOIT have made investigations on the metab- olism of fat with an exclusively albuminous diet. These investi- gations have shown that by increasing the quantity of proteids in the food the daily metabolism of fat decreases, so that indeed a formation of fat from proteids, or more correctly an excess of car- bon in the food as compared to the excretions, is produced, and this excess is considered as indicating a formation of fat from pro- teids. To illustrate this the following series of experiments were made on dogs, which were fed during seven different periods with increasing quantities of meat. Here as well as in the following pages the signs + and — represent an increase or a loss of the sub- stances under consideration. TABLE V. Flesh Flesh Fat Given. Decomposed. on the Body. 165 - 165 - 95 500 599 - 99 -47 1000 1079 - 79 - 19 1500 1500 .... +4 1800 1757 -f-43 -f 1 2000 2044 - 44 +58 2500 2512 - 12 +57 In these cases, according to the generally-received opinion, a formation of fat from proteids has taken place, even though it is small as compared to the quantity of decomposed proteid. In this formation of fat the proteids of the organism split into a nitrogen- ized part which ultimately yields urea, uric acid, etc., and a non- nitrogenized part which passes into fat or fat-forming substances. This non-nitrogenized part first diminishes the metabolism of fat and then, when it is formed in greater quantities, it is stored up as adipose tissue. The accumulation of fat in the body is therefore only small with an exclusively meat diet. The same is true also of the storing up of proteids, which decreases from day to day and which only lasts for a short time, because nitrogenous equilibrium soon occurs. This is also the reason why with an exclusively meat diet a well- EXCHANGE OF MATERIAL. 457 nourished body can be kept in its ordinary condition, while a poorly -nourished or diseased organism cannot be made fat. The very considerable elimination of nitrogen and the peculiar- ities which it presents in an exclusively meat diet have, together with other circumstances, among which is the unequal behavior of the metabolism of proteids on the first and the following days of starvation, led to the view (Vorr) that all proteids in the body are not decomposed with the same ease. VOTT differentiates the proteids fixed in the tissue-elements, so-called organized proteids, tissue- proteids, from those proteids which circulate with the fluids in the body and its tissues and which are taken up by the living cells of the tissues from the interstial fluids washing them. These circu- lating proteids are, according to VOIT, more easily and quickly destroyed than the tissue-proteids. Although in a fasting animal which has been previously fed with meat an abundant and quickly- decreasing decomposition of proteids takes place, while in the fur- ther course of starvation this proteid metabolism becomes less and more uniform, still this depends upon the fact that the supply of circulating proteids is diminished chiefly in the first days of starva- tion and the tissue-proteids in the last days. The tissue-elements constitute an apparatus of a relatively stable nature, which has the power of taking proteids from the fluids washing the tissues and digesting them, while a few proteids, the tissue-proteids, are ordinarily only disorganized to a small extent, about \% daily (Voix). By an increased supply of proteids the activity of the cells and their ability to decompose nutritive pro- teids is also increased to a certain degree. When nitrogenous equilibrium is obtained after increased supply of proteids, it denotes that the decomposing power of the cells for proteids has increased so that the same amount of proteids is metabolized as is supplied to the body. If the proteid metabolism is decreased by the simul- taneous administration of other non-nitrogenized foods (see below), a part of the circulating proteids may have time to become fixed and organized by the tissues, and in this way the mass of the flesh of the body increases. During starvation or with lack of proteids in the food the reverse takes place, for a part of the tissue proteids is converted into circulating proteids which are metabolized, and in this case the flesh of the body decreases. 458 PHYSIOLOGICAL CHEMISTRY. VOIT'S doctrine of the circulating and tissue proteids is indeed a hypothesis, but it explains in a satisfactory manner a number of otherwise very intricate relations and it agrees best with the facts. This theory has received further confirmation from the investiga- tions of several others, as PANTJM, FALCK and FEDER, on the tem- porary secretion of urea after food rich in proteids. From the investigations on a dog it was found that the secretion of urea increases almost immediately after a meal rich in proteids, and that it reaches its maximum in about six hours, when about one half of the administered proteids is secreted as the corresponding quantity of urea. If we also recollect that, according to an observation of SCHMIDT-MULHEIM on a dog, about 33$ of the given proteids are absorbed in the first two hours after the meal and about 56$ in the course of the first six hours, we may then infer that the increased elimination of nitrogen after a meal is due to a metabolization of the digested and absorbed proteids of the food. If we admit that the destroyed proteid must have been organized, then the greatly increased elimination of nitrogen after a meal rich in proteids sup- poses a far more rapid and comprehensive destruction and recon- struction of the tissues than has been generally admitted. VOIT'S theory on the different behavior of the tissue proteids and the circulating proteids in the animal body seems to have found support in the investigations of LUDWIG and TSCHIRJEW and of FORSTER. In TSCHIRJEW'S experiments a dog was given at one time boiled dog's blood, and at another time the same quantity of defibrinated dog's blood was injected into a vein. In the latter case the secretion of urea was very little increased, while in the former case it increased proportionally to the food consumed. In FORS- TER'S experiments, on the contrary, a dog was transfused with defibrinated dog's blood and also with horse- or dog-blood serum. After the transfusion of the blood the secretion of nitrogen was somewhat greater than in starvation, but the increase was only inconsiderable. In two experiments, in which 395 and 611 grms. of blood were transfused, the increase was only 3.6 and 3.4 grms. urea, while the proteids contained in the transfused blood corre- sponded to 32 and 42 grms. urea. After the tranf usion of 522 grms. of dog's-blood serum, in which the quantity of proteids corre- sponded to 10.6 grms. urea, the elimination of urea was only 6.4 EXCHANGE OF MATERIAL. 459 grins, greater on the day of the transfusion than on the following day. According to these experiments, the proteids of the blood - corpuscles, which are considered as tissue-proteids, are less easily metabolized than digested blood-proteids or the proteids of the blood-serum, and therefore these experiments confirm VOIT'S views. It has been stated above that other foods may decrease the destruction of proteids. Gelatine is such a food. Gelatine and the gelatine-formers do not seem to be converted into proteid in the body, and this last cannot be entirely replaced by gelatine in the food. For example, if a dog is fed on gelatine and fat, its body sustains a loss of proteids even when the quantity of gelatine is so large that the animal, with an amount of fat and meat containing just the same quantity of nitrogen as the gelatine in question, may remain in nitrogenous equilibrium. On the contrary, gelatine, as VOIT, PAJ^UM and OEBUM have shown, has a great value as a means of sparing the proteids, and, according to VOIT, it may decrease the decomposition of proteids to a still greater extent than fats and carbohydrates. This is apparent from the following summary of VOIT'S experiments on a dog : TABLE VI. Food per Day. Flesh. Meat. Gelatine. Fat. Sugar. Decomposed. On the Body. 400 0 200 0 450 - 50 400 0 0 250 439 - 39 400 200 0 0 256 -f 44 This ability of gelatine to spare the proteids is explained by VOIT by the statement that the gelatine is decomposed instead of a part of the circulating proteids, whereby a part of this last may be organized. Gelatine may also decrease somewhat the consumption of fat, although it is of less value in this respect than the carbohydrates. The question of nutritive value of peptones stands in close rela- tion to the nutritive value of the proteids and gelatine. The early investigations made by MALY, PLOS'Z and GYERGYAY, and ADAM- KIEWIC'Z have led to the conclusion that an animal with food which 460 PHYSIOLOGICAL CHEMISTRY. contains no proteids besides peptones may not only preserve its nitrogenous equilibrium, but indeed its proteid condition may in- crease. In opposition to this VOIT believes, basing his^opinion upon some recent experiments conducted by FEDER, that the peptones are completely destroyed in the body; that indeed by their ability of sparing the proteids they entirely, or almost entirely, prevent the consumption of proteids, but cannot pass into proteid. The gen- eral view is still that (see page 231) peptones are reconverted into proteids in the body. In the above-mentioned experiments with peptones a mixture of albumoses and peptones in the modern sense was used. Recently ZUNTZ and POLLITZER have made experiments on dogs partly with meat and partly with pure peptones and albumoses of various kinds. In these experiments a deposit of proteids (retention of a part of the nitrogen) seems to have taken place in the body, and if a cor- rection be made for that nitrogen which is contained in the extrac- tive bodies of the meat, then the investigated digestive products seem to have about the same nutritive value for the body as the corresponding quantity of proteids of the meat. From experiments made by WEISKE on herbivora it appears that asparagin may spare albumin in such animals. In carnivora (J. MUNK) and in mice (VoiT and POLITIS) it was found that asparagin does not seem to have any sparing action on the proteids. It is not known how it acts in man. Exchange of Material on a Diet consisting of Proteid and Fat. Fat cannot arrest or prevent the destruction of proteids; but it can decrease it, and so spare the proteids. This is apparent from the following table of VOIT. A is the average for three days, and B for six days. TABLE VII. Food. Flesh. Meat. Fat. Metabolized. On the Body. A.... 1500 0 1512 - 12 B. . . . 1500 150 1474 + 26 The adipose tissue of the body acts like the food-fat, and the proteid-sparing eifect of the former may be added to that of the latter, so that a body rich in fat may not only remain in nitrogenous EXCHANGE OF MATERIAL. 461 equilibrium, but may even add to the store of bodily proteids, while in a lean body with food containing the same amount of proteids and fat there would be a loss of proteids. In a body rich in fat a greater amount of proteids is protected from metabolism by a cer- tain quantity of fat than in a lean body. Because of the sparing action of fats an animal by the addition of fat to its food may, as is apparent from the tables, increase its proteid condition with a quantity of meat which is insufficient to preserve nitrogenous equilibrium. The proportion of proteids and fats in the food is of the greatest importance in regard to in- creasing the proteids of the body. With an exclusively proteid diet the metabolism of proteids increases with the increased amount of proteids in the food, and this behavior is not prevented by the addition of fat to the food, even though the absolute extent of the metabolism of proteids is somewhat diminished. In the presence of a great deal of proteid in proportion to the fat of the food, very large quantities of proteid are necessary for the maintenance of nitrogenous equilibrium, as well as for an increase of proteid, while by the addition of a relatively large amount of fat to the proteids such a result is reached even with comparatively small amounts of proteids. The following table will elucidate this point: TABLE VIII. Food. Flesh. Meat. Fat. Metabolized. On the Body. 450 250 344 -f- 106 1000 250 875 +125 1500 250 1381 +119 The table shows that also in the presence of fat the proteid de- struction decreases with increased amounts of proteids in the food, and that, because of this, in the given examples no essentially in- creased metabolism of proteid is obtained with a diet of 1500 grms. meat and 250 grms. fat than with 450 grms. meat and the same quantity of fat. The importance of this fact will be spoken of later. If the quantity of meat in the food is kept constant by feeding with meat and fat, while the fat varies, then the destruction of pro- 462 PHYSIOLOGICAL CHEMISTRY. teids may gradually decrease with increased quantity of fat. This does not always take place, but on feeding with increased amounts of carbohydrates it does (Vorr). The same sparing action on proteids which the neutral fats have occurs, according to J. MUNK, also with the fatty acids, while the second chief constituent of the neutral fats, glycerine, when it is taken in quantities equal to 1-2 grms. per kilo of the body does not seem to have any influence on proteid metabolism (J. MUKK). In regard to the metabolism of fat, it has been found that with a constant quantity of proteid in the food the metabolism of fat in- creases with variable amounts of absorbed fats. The following table seems to show this : TABLE IX. Food. Fat. Meat. Fat. Metabolized. On the Body. 500 0 47 -47 500 100 66 +34 500 200 109 +91 The fat of the body acts like the fat of the food. A body rich in fat decomposes a greater fraction of fat than a lean body, and the same quantity of absorbed fat of the food, which in a fat body is completely decomposed, may in a lean body cause a deposit of fat. If the body takes greater amounts of fat and proteids than it can decompose in the same time, then with increasing amounts of absorbed fats the fraction of the same which is deposited in the body also increases (see Table IX). The greatest deposit of pro- teids and fat occurs after taking medium quantities of both in proper proportion to each other (see below). Exchange of Material with a Diet consisting of Proteids and Carbohydrates. That which has been said above in regard to the action of fats on the metabolism of proteids answers essentially also for the carbohydrates. The carbohydrates may also spare the proteids. By the addition of carbohydrates to the food the carnivora not only remains in nitrogenous equilibrium, but the same quantity of meat which in itself is insufficient and which without carbo- hydrates would cause a loss of weight in the body may with the addition of carbohydrates produce a deposit of proteids. The EXCHANGE OF MATERIAL. 463 carbohydrates have a stronger sparing action on proteids than fats (VoiT). This is apparent from the following table : TABLE X. Food. Flesh. Meat. Fat. Sugar. Starch. Metabolized . On the Body. 500 250 558 - 58 500 . 300 466 + 34 500 . . 200 , 505 - 5 800 250 745 -1- 55 800 200 773 + 27 2000 . . . . 200-300 1792 + 208 2000 250 1883 + 117 Because of the great sparing action of carbohydrates on the proteids, the herbivora, whose food generally contains large quanti- ties of carbohydrates, easily increase in proteids (VoiT). While with the same amount of flesh increased amounts of fat in the food do not continuously decrease the destruction of proteids, according to VOIT the carbohydrates habitually decrease the metabolism of proteids. This is seen from the previous table, but is more apparent from the following : TABLE XL Food. Flesh . Meat. Carbohydrates. Metabolized. On th'e Body. 500 100 537 - 37 500 200 505 - 5 500 300 466 + 34 2000 100 1847 + 153 2000 200 1778 + 222 2000 200 1780 +220 With the addition of carbohydrates to the food the destruction of proteids increases with an increased amount of proteids. In the presence of only small amounts of carbohydrates very large amounts of proteids are necessary in the food to produce an increase in the proteids of the body, while the result is more simply and advantageously attained by considerably less proteids and propor- tionally more carbohydrates. The action of the carbohydrates on the accumulation of fat has been demonstrated by the investigations of VOIT and PETTEN- KOFER showing that the carbohydrates not only decrease the metabolism of fat and prevent its loss, but also cause its accumu- 464 PHYSIOLOGICAL CHEMISTRY, lation. The various views in regard to the importance of the carbohydrates in the formation of fat which have been suggested from time to time have been given on page 249, and it is there stated that, according to the present view, the carbohydrates not only spare the fat, but may also be converted into fat in the body. The very important question as to the conditions for the depo- sition of fat and flesh in the body, stands in close relation to what has just been said in regard to food consisting of proteids and carbohydrates. In the previous pages it was repeatedly stated that an exclusive diet rich in proteids causes an increased destruction of proteids, and that such a diet soon produces a nitrogenous equilibrium. By the exclusive increased administration of proteids the proteid condition of the body may be increased only for a short time and to a small extent, and this only when the body was previously proportionally well fed. This does not occur in bodies poor in fat from disease or from some other cause. If we wish to cause a deposition of pro- teids in the body, then we must administer in sufficient amounts with the food, besides proteids, also other bodies which will spare the proteids, such as gelatine, fat, or carbohydrates^ and indeed, for several reasons, chiefly fat and carbohydrates. It has also been previously stated that, because of the property of the proteids to raise the proteid metabolism, an accumulation of proteids may be caused more economically and better with a medium amount of proteids and proportionally more non-nitrogen ized sub- stances than with a greater amount of proteids and proportionally less non-nitrogenized bodies. Above all, such a proper relation between proteids and non-nitrogenized bodies is important when we aim at keeping the deposit of flesh for a longer time. The follow- ing extracts from VOIT'S tables are instructive in this regard : TABLE XII. Number of Days F°od' Total Deposit of Nitrogenous of Experimentation. 'Meat> grms< Fat> grm"^ Flesh, grms. Equilibrium. 32 500 250 1794 not yet 3 750 250 271 near 3 1000 250 375 4 1500 250 476 7 1800 250 854 nitrogenous equilibrium 3 2000 250 352 near EXCHANGE OF MATERIAL. 465 The greatest absolute deposition of flesh in the body was obtained in these cases with only 500 grms. flesh and 250 grms. fat, and even after 32 days the nitrogenous equilibrium had not occurred. On feeding with 1800 grms. meat and 250 grms. fat the nitrogenous equilibrium occurred after 7 days ; and though the deposition of flesh per day was greater, still the absolute deposit was not one half as great as in the former case. Inasmuch as the quantity of pro- teids does not decrease below a certain amount, it seems that the most abundant and most lasting deposition of flesh is obtained with a food which does not contain too much proteids in proportion to the fat. The same is also true of a diet consisting of proteids and carbohydrates. From the above conditions concerning a deposit of fat in the body it follows that such a deposit may indeed occur with an exclusively flesh diet, but that in this case it is only very small even when large amounts of proteids are taken. For the production of an abundant deposit of fat the body must take with the food, besides proteids, either fats or carbohydrates or, which is best for human beings, fat and carbohydrates simultaneously. The carbohydrates are of special importance because they are generally cheaper in comparison with the fats. As the non-nitrogenized bodies are, according to all appearances, the most important source of muscular activity, then diminished bodily work or rest must be a favorable condition for the deposition of fat in the body. Best, with a proper combination of the three chief groups of organic food, is therefore of great importance in fattening, the object being to cause as great an increase as possible in the mass of the proteids and fats in the body in the cheapest way. Action of certain other Bodies on the Exchange of Material. Water. If a quantity in excess of that which is necessary is intro- duced into the organism, the excess is quickly and principally eliminated with the urine.. This increased elimination of urine causes in fasting animals (VoiT, FORSTER), but not to any mention- able degree in animals taking food (SEEGEN*, MUNK, MAYER), an increased elimination of urea. The reason for this increased elimi- nation is sought for in the fact that the abundant drinking of water causes a complete washing out of the urea from the tissues. An- other view, which is defended by VOIT, is that because of the more 466 PHYSIOLOGICAL CHEMISTRY. active current of fluids after taking large quantities of water an increased metabolism of proteids takes place. VOIT considers this explanation is the correct one, although he does not deny that by the abundant- administration of water a more complete washing out of the urea from the tissues takes place. In regard to the action of water on the formation of fat and its metabolism, the view that abundant drinking of water is favorable for the deposition of fat seems to be generally admitted, while taking only very little water acts against its formation. Salts. The excretion of urine, even when no great quantities of water are taken, is increased by common salt, and the elimina- tion of urea is also increased at the same time. The same two possibilities may be considered for this last as in the action of water on the excretion of urea. The experiments continued for a long time by VOIT, in which the absolute increase of the elimination of urea was considerable (106 grms. in 49 days), render the conclusion probable that common salt somewhat increases the metabolism of the proteids. Certain other salts, such as potassium chloride, sodium sulphate, sodium phosphate, acetate, saltpetre, and ammonium chloride, also seem to act like common salt. Sodium borate and the sodium salts of salicylic and benzoic acids also seem to have an increased action on the metabolism of proteids. Alcohol. The question as to how far the alcohol absorbed in the intestinal canal is burnt in the body, or whether it leaves the body unchanged by various channels, has been the subject of much dis- cussion. To all appearances the greatest part of the alcohol is burnt. According to BODLANDER, 1.18$ of the alcohol taken is eliminated with the urine, 0.14$ by the evaporation from the skin, and 1.6$ with the expired air. The remainder, or about 97$, is burnt in the body. As the alcohol is in greatest part burnt in the body, then the question arises whether it acts sparingly on other bodies, and whether it is to be considered as a nutritive body. The investiga- tions made to decide this question have led to no decisive result. In the experiments on the elimination of nitrogen in human beings sometimes a diminished (HAMMOND, E. SMITH, OBERNIER), some- times an unchanged (PARKES and WOLLOWICZ), and in other cases an increased (FORSTER and ROMEYN) elimination of nitrogen was observed after the administration of small amounts of alcohol. EXCHANGE OF MATERIAL. 467 FOKKER and J. MUNK after the administration of small amounts of alcohol to dogs found a diminished, and after large amounts an increased, metabolism of proteids. Many observations have been made in regard to the extent of exchange of gas after taking alcohol. BOECK and BAUER observed in dogs after giving small amounts of alcohol that the consumption of oxygen as well as the elimination of carbon dioxide was increased. BODLANDER found in rabbits and dogs a decrease in the consump- tion of oxygen and the elimination of carbon dioxide, while WOL- FERS found an increased consumption of oxygen in rabbits. In an investigation on the human body ZUNTZ and BERDEZ, and also GEPPERT, observed no essential change in the respiratory exchange of gas after small, non-intoxicating doses of alcohol. As alcohol is in greatest part burnt up in the body and the exchange of gas is nevertheless not essentially raised, it seems as if the alcohol dimin- ishes the combustion of other bodies and thereby has a sparing value. This value may still, if it really has such an action, be of essential importance only in certain cases, as large quantities of alcohol taken at once or the continued use of smaller quantities has injurious action on the organism. Alcohol may therefore be con- sidered as a nutritive body in exceptional cases only, and it other- wise must be considered as an article of luxury. Coffee and tea have no positively proved action on the exchange of material, and their importance lies chiefly in their action upon the nervous system. IV. The Dependence of the Exchange of Material on Other Conditions. Rest and Work. According to LIEBIG, muscular activity is con- nected with an increased metabolism of proteids, but, as has been mentioned on page 267, the investigations of others, especially VOIT on dogs, and of PETTENKOFER and VOIT on man, show that the total elimination of nitrogen during activity or as a result of the same is not mentionably increased. It is indeed true that certain investigators have observed an increased elimination of nitrogen in special cases; but this increase has been explained in other ways. Work may, for instance, when it is connected with violent 468 PHYSIOLOGICAL CHEMISTRY. movements of the body, easily cause dyspnoea, and this last may, as FRANKEL has shown, since diminution of the oxygen supply in- creases the proteid metabolism, cause an increase in the elimination of nitrogen. In other series of experiments the quantity of carbo- hydrates and fats in the food was not sufficient; the supply of fat in the body was decreased thereby, and the destruction of proteid s was also correspondingly increased. Work may also increase the appetite, and an increase in the elimination of nitrogen may be caused by the greater quantity of proteids taken. According to the generally-accepted views, muscular activity has hardly any in- fluence on the metabolism of proteids. On the contrary, it has a very considerable influence on the metabolism of non-nitrogenized bodies and — as a measure of the extent of the metabolism — on the elimination of carbon dioxide and the consumption of oxygen. This action, which was first observed by LAVOISIER, has recently been confirmed by many in- vestigators. PETTENKOFER and VOIT have made investigations as to the metabolism of the nitrogen ized as well as of the non- nitrogenized bodies during rest and work, partly while fasting and partly on a mixed diet. The experiments were made on a full-grown man weighing 70 kilos. The results are contained in the following table : TABLE XIII. Consumption of , ---- • ----- , Water Proteids. Fat. Carbohydrates. CO2 eliminated. O consumed, expired. •tfoot^r j Rest.. 78 215 ... 716 761 889 casting... - Work 75 380 1187 TIT j ,,- ,j Rest.. 137 65 352 912 831 828 et \ Work 137 173 352 1209 980 1412 The work in this case had no influence on the destruction of proteids, while the consumption of non-nitrogenized bodies and the elimination of water by the skin and lungs was considerably increased. The question of the exchange of material in sleep and waking stands in close relationship to the exchange of material in rest and activity. According to PETTENKOFER and VOIT, the metabolism of proteids is not constantly influenced by these two different condi- tions; on the other hand, the production of carbon dioxide is habitually greater during the day than in the night. If excessive EXCHANGE OF MATERIAL. 469 work has been done during the day, the elimination of carbon dioxide may be decreased during the following night. In sleep the metabolism of non-nitrogenized substance is indeed smaller than in rest without sleep (LEVIN), and it is less the more sound the sleep. The reason for the less abundant elimination of carbon dioxide in sleep does not only depend upon muscular rest but also on several other conditions, among which are the absence of light arid other excitants which act in the day and which, as it seems, cause a reflex of the chemical tonus of the muscles and thereby produce an exchange of material. Such a regulation of the exchange of material and the production of heat brought about by the nerves of the skin which is produced by the influence of the chemical tonus of the muscles shows that the external temperature is of the greatest importance in the exchange of material. Action of the temperature of the surrounding air. In cold- blooded animals the production of carbon dioxide increases and decreases with the rise and fall of the surrounding temperature. In warm-blooded animals this condition is the reverse. By the investigations of LUDWIG and SANDERS-EZN, PFLUGER, DUKE CHARLES THEODORE OF BAVARIA, and others, it has been demon- strated that in warm-blooded animals the change in the external temperature has different results, according as the animal's own heat remains the same or changes. If the temperature of the animal sinks, the elimination of carbon dioxide also sinks; if the temperature rises, the elimination of C02 also rises. If, on the contrary, the temperature of the body remains unchanged, then the elimination of carbon dioxide increases with a lower and decreases with a higher external temperature. This condition may be ex- plained, according to PFLUGER and ZUNTZ, by the statement that the low temperature, by exciting a reflex action in the sensitive nerves of the skin, causes an increased metabolism of the muscles with an increased production of heat affecting the temperature of the body. The increased exchange of material produced at a low external temperature only applies, as far as is known, to the non- nitrogenized substances, but not to the proteids. Weight of Body and Age. The greater the mass of the body the greater the absolute consumption of material; while, other things 470 PHYSIOLOGICAL CHEMISTRY. being equal, on the contrary, as above stated in speaking of the exchange of material in starvation, a small individual of the same species of animal, because of its relatively greater surface of body and therefore its relatively greater surface of heat, decomposes relatively more substance. With about the same bodily weight the destruction of proteids is smaller with a greater amount of fat. In women, who generally have less bodily weight and greater amount of fat than men, the consumption of proteids and the metabolism of material in general is therefore smaller, and the latter is ordinarily about ^ of that of men. Otherwise the sex does not seem to hrve any special influence on the exchange of material. Young animals have, for the reason above mentioned (page 444), a greater exchange of material than older ones, and they decompose a greater quantity of substance per kilo. In regard to the exchange of material in children the investigations of SCHARLING and FOR- STER on the elimination of carbon dioxide, and of CAMERER on the elimination of urea, are important. FORSTER found in children at the ages given and at rest the following elimination of carbon dioxide per kilo in one hour: 3-5 vears 1.17 grms. CO3 6-7" " 1.17 " 9-13 " , . . . 0.90 " In a grown man at rest PETTENKOFER and VOIT found the elimination of carbon dioxide with a mixed diet was 0.55 grm. per kilo in one hour. In children of 3 to 7 years the elimination of C02 per kilo is more than double that of grown persons. At the age of 16 years the elimination of carbon dioxide per kilo is about the same as in grown persons. CAMERER has found the following results as to the elimination of urea in children : TABLE XIV. Age. Weight of Body in Kilos. Urea in grms. Per Day. Per Kilo. 7 months 6.50 5.0 0.75 H years 895 12.1 1.85 3 4 5 7 9 12* 15 1261 11.1 090 17.43 14.6 0.84 16.20 12.3 0.76 18 80 13.9 0.74 25.10 17.3 0.69 32 60 17.6 0.54 35.70 17.9 0.50 EXCHANGE OF MATERIAL. 471 In adults weighing about 70 kilos about 30 to 35 grms. urea per day are eliminated, or 0.5 gnu. per kilo. At about 15 years of age the destruction of proteids per kilo is about the same as in adults. The relatively greater metabolism of proteids in young individuals is explained partly by the fact that the metabolism of material in general is more active in young animals, and partly by the fact that the young animal is as a rule poorer in fat than the grown ones. V. Potential Energy and the Relative Nutritive Value of Various Organic Foods. With the organic foods the organism receives a supply of poten tial energy which is converted into living force in the body. This potential energy of the various foods may be represented by the amount of heat which is set free in their combustion. This quan- tity of heat, expressed in calories, if we consider the calorie as that quantity of heat which is necessary to raise 1 grm. of water from 0° C. to 1° 0., is the following for 1 grin, of the substance. TABLE XV. Calories in Calories in Dry Substance. Ash-free Substance. Proteids (in meat) 5754 5778 ) Muscle 5345 5656 [• RUBNER. Fat (pig-fat, melt, pt. -f 43°) 9423 ) Grape-sugar 36921 Milk-sugar 3877 I STOHMANN Cane-sugar 3959 ^TOHMANN. Starch 4116 J Fat and carbohydrates are completely burnt in the body, and we can therefore consider their combustion equivalent as a measure of the living force developed by them within the organism. The pro- teids act differently. They are only incompletely burnt, and they yield certain products of decomposition which, leaving the body with the excreta, still represent a certain amount of potential energy which is lost for the body. The heat of combustion of the proteids is smaller within the organism than outside of it, and they must therefore be specially determined. For this purpose RUBNER fed a dog on washed meat. He subtracted from the heat of com- 472 PHYSIOLOGICAL CHEMISTRY. bustion of the food the heat of combustion of the urine and faeces, which corresponded to the food taken plus the quantity of heat necessary for the swelling up of the albuminous bodies and the solution of the urea. RUBBER has also tried to determine the heat of combustion of the proteids (inuscle-proteids) decomposed in the body of rabbits in starvation. According to these investigations, the physiological heat of combustion in calories for each gramme of substance is as follows : TABLE XYI. 1 grm. of the Dry Substance. Calories. Proteids from meat 4424 Muscle 4000 Proteids in starvation B842 Fat (average for various fats) 9300 Carbohydrates (calculated average) 4100 The physiological combustion value of the various foods belong- ing to the same group is not quite the same. It is, for instance, 3969 calories for a vegetable albuminous body, conglutin, and 4424 calories for an animal albuminous body, syntonin. According to BUSIER, we may consider the normal heat value per 1 grm. of ani- mal proteid as 4233 calories, and of vegetable proteid as 3960 calo- ries. When a person on mixed diet takes about 60$ of the proteids from animal foods and about 40$ from vegetable foods, then we may consider as the value of 1 grm. of the proteid of the food as about 4100 calories. The physiological value for each of the three chief groups of organic foods, by their decomposition in the body, is in round numbers as follows : TABLE XVII. Calories. 1 grm. proteid = 4100 1 " fat =9300 1 " carbohydrate = 4100 As above stated several times, the fats and carbohydrates may decrease the metabolism of proteids in the body, while, on the other hand, the quantity of proteids in the body or in the food acts on the metabolism of fat in the body. In the physiological combustion the various foods may replace one another to a certain extent, and it is therefore important to know in what proportion they replace one another. The investigations made by RUBHER have taught us FOOD NEEDED BT MAN UNDER VARIOUS CONDITIONS. 473 that when we wish to diminish the loss of fat or to accumulate fat it takes place in proportions that correspond to the figures of the heat value of the same. This is apparent from the following table. In this we find the weight of the various foods equal to 100 grms. fat, a part determined from experiments on animals and a part calculated from figures of the heat values. TABLE XVIII. 100 grms. fat are equal, to or isodynamic with : From Experiments From the Difference, on animals. Heat Value. per cent. Syntonin 225 213 +5.6 Muscle-flesh (dried).. 243 235 -j-4.3 Starch 232 229 -f 1.8 Cane-sugar 234 235 — 0 Grape-sugar 256 253 - 0 From the given isodynamic value of the various foods, it follows that these substances replace one another in the body almost in exact ratio to the potential energy contained in them. Thus in round numbers 240 grms. carbohydrate are equal to or isody- namic with 100 grms. fat, but only in regard to its ability to pre- vent the loss of fat. In regard to the sparing of proteids the carbohydrates accomplish more than the same quantity of fat (page 463). The knowledge of these isodynamic values, as well as of the potential energy in the various foods, is of fundamental impor- tance in the calculation of the diet of human beings under various conditions. VI. The Need of Man for Food under Various Conditions. Various attempts have been made to determine the daily amount of organic food needed by man. Certain investigators, such as PLATFAIB, MOLESCHOTT, and others, have, from the total consump- tion of food by a large number of similarly-fed individuals, soldiers, sailors, laborers, etc., calculated the average quantity of food required per head. Others, such as PARKES, SMITH, and VOIT, have calculated the daily demand of food from the quantity of carbon and nitrogen in the excreta. Others again, as PETTEK- KOFER and VOIT, have calculated the quantity of nutritive material in a diet by which an equilibrium was maintained in the individual for one or several days between the consumption and elimination of carbon and nitrogen. Lastly, others, especially FORSTER, have 474 PHYSIOLOGICAL CHEMISTRY. quantitatively determined during a period of several days the organic nutritive substances consumed daily by persons choosing their own food, who were employed in various industries, and on which they felt well and fully capable of labor. Among these methods a few are not quite free from reproach and others have not as yet been tried on a sufficiently large scale. Nevertheless the experiments collected thus far serve, partly be- cause of their number and partly because of the methods, to correct and control one another, and also serve as a good starting-point in determining the diet of various classes and similar questions. If we convert the quantity of nutritive substance daily taken into calories, produced during physiological combustion, we then obtain some idea of the sum of the chemical tension which under different conditions is introduced into the body. It must not be forgotten that the food is never completely absorbed and that undigested or unabsorbed residues are always expelled from the body with the faeces. The gross results of calories calculated from the food taken must therefore, according to RUBNER, be dimin- ished at least 8#. The following summary contains certain examples of the quantity of food which is consumed by individuals of various classes under different conditions. In the last column we also find the amount of living force, calculated as calories, which corresponds to the quantity of food in question with the above-stated correction. The calories are therefore net results, while the figures for the nutritive bodies are gross results. TABLE XIX. Proteids. Fat. Carbohydrates. Calories. Authority. Soldier during peace... 119 40 529 2784 PLAYPAIR. light service... 117 35 447 2424 HILDESHEIM. " infield 146 44 504 2852 Laborer 130 40 550 2903 MOLESCHOTT. at rest 137 72 352 2458 PETTENKCFEK & Vorr. Cabinet-maker (40 years) 131 68 494 2835 FORSTER. Young physician 127 89 362 2602 134 102 292 2476 Laborer 133 95 422 2902 English smith 176 71 6H6 3780 PLAYPAIR. " pugilist 288 88 93 2189 Bavarian wood -chop per 135 208 876 5589 LIEBIG. Laborer in Silesia 80 16 552 2518 MEINERT. Seamstress in London. . 54 29 292 1688 PLAYFAIR. FOOD NEEDED BY MAN UNDER VARIOUS CONDITIONS. 475 It is evident that persons of essentially different weight of body who live under unequal external conditions must need essentially different food. It is also to be expected (and this is confirmed by the table) that not only the absolute quantity of food consumed by various persons, but also the relative proportion of the various organic nutritive substances, shows considerable variation. Results for the daily need of human beings in general cannot be given. For certain classes of human beings, such as soldiers, laborers, etc., results may be given which are valuable for the calculation of the daily rations. Based on extensive investigations and a very wide experience, VOIT has proposed the following average quantities for the daily diet of adults : Proteids. Fat. Carbohydrates. Calories. For men 118 grms. 56 grms. 500 grms. 2810 But we must here remark that these statements relate to a man weighing 70 to 75 kilos and who was engaged daily for ten hours with not too fatiguing labor. The amount of food required by a woman engaged in moderate labor is about £ that of a laboring man, and we may consider the ^ollowing as a daily diet with moderate work. Proteids. Fat. Carbohydrates. Calories. For women . . 94 grms. 45 grms. 400 grms. 2240 The proportion of fat to carbohydrates is here as 1:8-9. Such a proportion occurs often in the food of the poorer classes, while the ratio in the food of wealthier persons is 1 : 3-4. The maximum quantity of carbohydrates in the food must, according to VOIT, be above 500 grms. ; and as the carbohydrates besides constitute the chief part of the often very bulky vegetable foods, it has been sug- gested and is desirable on this and other grounds to increase the quantity of fat at the expense of the carbohydrates in such rations. But because of the high price of fat such a modification cannot always be made. In judging the above numbers for the daily rations it must not be forgotten that the figures for the various nutritive bodies are gross results. They consequently represent the quantity of the 476 PHYSIOLOGICAL CHEMISTRY. nutritive Bodies which must be taken in, and not those which are really absorbed. The figures for the calories, which here and in the following pages are so-called gross calories are, on the contrary, net results. The various foods are, as is well known, not equally digested and absorbed, and in general the vegetable foods are less completely used up than animal foods. This is especially true of the proteids. When, therefore, VOIT, as above stated, calculated the daily amount of proteids needed by a laborer as 118 grms., he starts with the supposition that the diet is a mixed animal and vegetable one, and also that of the above 118 grms. about 105 grms. are absorbed. The results obtained by PFLUGER and his school, BLEIBTREU and BOHLAND, for the extent of the metabolism of proteids in man with a diet optional and sufficient correspond well with the above figures — when the unequal weight of the body of the various persons experimented upon is sufficiently considered. As a rule, as a more exclusively vegetable food is employed, the amount of proteids in the same is also habitually smaller. The strictly vegetable diet of certain people — as of the Japanese — and that of the so-called vegetarians is therefore a proof that a person, if the quantity of food be sufficient, may exist on considerably smaller quantities of proteids than VOIT suggests. It follows from the investigations of HIRSCHFELD and KUMAGAWA that a nearly complete or indeed a complete nitrogenous equilibrium may be attained by the sufficient administration of non-nitrogenized nutri- tive bodies with relatively very small amounts of proteids. Hirsch- feld, who weighed 73 kilos, could maintain nitrogenous equilibrium very nearly with a diet containing 43.5 grms. nitrogeriized bodies, 165 grms. fat, 354 grms. carbohydrates, and 42.7 grms. alcohol. Kumagawa made experiments on himself with a purely vegetable diet, consisting mainly of boiled rice. On an average 50.5 grms. proteids and 569.83 grms. carbohydrates were daily introduced, and of this 37.82 grms. proteids and 566.7 grms. were daily used up By this food he was not able with a bodily weight of 48 kilos to attain nitrogenous equilibrium, but he found indeed that a part of the nitrogen was retained in the body. The weight of the body increased and the general condition was good. The total amount of calories in the absorbed food was in this case 2500 in round num- FOOD NEEDED BY MAN UNDER VARIOUS CONDITIONS. 477 bers, or 52 per kilo. It follows from the experiments just men- tioned that an adult may be sufficiently nourished with a consider- ably smaller quantity of proteids than VOIT considers necessary, if the total food corresponds to the demands of the body in calories, which may be accomplished by a corresponding increase in the ad- ministration of non-nitrogenized nutritive bodies. If we keep in mind that the food of people of different coun- tries varies greatly, and that the individual also takes essentially different nourishment according to the external conditions of liv- ing and the influence of climate, it is not remarkable that a person accustomed to a mixed diet cannot exist for a long time on a strictly vegetable diet deficient in proteids, even though not especially difficult to digest. No one doubts the ability of man to adapt himself to a heterogeneously-composed diet when this is not too dif- ficult of digestion and is sufficient; but this ability does not seem sufficient reason for essentially altering the figures suggested by VOIT. VOIT'S figures are based on comprehensive experiments, also- on experience and exact knowledge of the actual existing condi- tions, and they are, which is especially important, as above stated, only given for certain cases or certain classes of people. It is not denied by any one that these results are not applicable to all cases, as it is evident that the daily ration necessary for a laborer given by VOIT must be altered somewhat for other countries because of the existing conditions in middle Europe, where VOIT made his in- vestigations. With regard to the conditions in Sweden we may propose as the daily diet of a laboring man about 120 grms. pro- teids, 100 grms. fat, and 400-450 grms. carbohydrates. Thes^ figures are based on the experience obtained in Sweden and cal- culations made by the author. If we compare the figures of Table XIX with the average figures proposed by VOIT for the daily diet of a laborer, it would seem at the first glance as if the consumed food in certain cases was con- siderably in excess of the need, while in other cases, as for instance for the seamstress in London, it was entirely insufficient. A posi- tive conclusion cannot, therefore, be drawn if we do not know the weight of the 'body as well as the labor performed by the person, and also the conditions of living. It is certainly true that the amount of nutriment required by the body is not directly propor- 478 PHYSIOLOGICAL CHEMISTRY. tional to the bodily weight, for a small body consumes relatively more substance than a larger one, and varying amounts of i'at may also cause a difference; but a large body, which must maintain a greater quantity, consumes an absolutely greater quantity of sub- stance than a small one, and in estimating the nutritive need one must also always consider the weight of the body. According to VOIT, the diet for a laborer with 70 kilos bodily weight requires 40 calories for each kilo. In KUMAGAWA'S series of experiments, in which the absolute quantity of food was smaller, the average was 52 calories for each kilo. As above stated several times, the demands of the body for nour- ishment vary with its different conditions. Among these condi- tions two are practically important, namely, labor and rest. In a previous chapter, in which muscular labor was spoken of, it was seen that the generally accepted view is that non-nitrogenized food is the most essential, if not the exclusive, source of muscular force. As a natural sequence it is to be expected that in activity the non-nitrogenized foods before all must be increased in the daily rations. Still this does not seem to correspond to daily experience. It is a well-known fact that hard-working individuals — men and ani- mals— require a greater amount of proteids in the food than less active ones. This contradiction is, however, only apparent, and it depends, as VOIT has shown, upon the fact that individuals used to violent work are more muscular. For this reason a person per- forming severe muscular labor requires food containing a larger pro- portion of proteids than an individual whose occupation demands less violent exertion. Another question is, how should the relative and absolute amount of food be changed if increased exertion be demanded of one and the same individual ? An answer based upon experience may be found in statistics concerning the maintenance of soldiers in peace and in war. Many such statements are obtainable. In a critical examination of the same it was found that in war-rations the quantity of non-nitrogen- ized bodies as compared to the proteids is only increased in excep- tional cases, while usually the reverse is the case. Even in these cases the actual proportion does not correspond to the theoretical demand upon which, however, too great stress must not be placed, FOOD NEEDED BY MAN UNDER VARIOUS CONDITIONS. 479 since in the case of soldiers in the field many other circumstances are to be considered, such as the volume and weight of the food, etc., etc., which cannot here be more closely discussed. The fol- lowing table shows the average results of soldiers' rations in war and peace, calculated by ALMEN from the detailed statement of several countries.1 These average results also include the figures for Sweden. TABLE XX. A. Peace Ration. B. War Ration. Proteids. Fat. Carb. Proteids. Fat. Carb. Minimum 108 22 504 126 38 484 Maximum 165 97 731 197 95 688 Meau 130 40 531 146 59 557 Sweden (proposed). 179 102 591 202 137 565 If we do not consider the very abundant rations proposed for the soldier in Sweden, and if we only adhere to the above mean figures, we obtain the following results for the daily rations: Proteids. Fat. Carb. Calories. In peace 130 40 531 2900 In war 146 59 557 3250 If we calculate the fat in its equivalent amount of starch, then the relation of the proteids to the non-nitrogenized foods is : In peace 1 : 4.97 In war 1 : 4.79 The proportion is nearly the same in both cases ; the small dif- ference which occurs shows a slight relative increase in the proteids in the war ration. On the contrary, what is especially apparent from the total of the calories, the total quantity of nutritive bodies is greater in the war than in the peace ration. As more work requires an increase in the absolute quantity of food, so the quantity of food must be diminished when little work is performed. The question as to how far this can be done is of importance in regard to the diet in prisons and poor-houses. We give below the following as example of such diets. 1 Germany, Austria, Switzerland, France, Italy, Russia, and the United States. 480 PHYSIOLOGICAL CHEMISTRY. TABLE XXI. Proteids. Fat. Carb. Calories. Prisoner (not working) 87 22 305 1667 SCHUSTER. 85 30 300 1709 VOIT. Man in poor-house 92 45 332 1985 FORSTER. Woman in " 80 49 266 1725 The figures given by VOIT are, according to him, the lowest fig- ures for a non- working prisoner. He considers the following as the lowest diet for old non-working people : Proteids. Fat. Carb. Calories. Men 90 40 350 2200 Women 80 35 300 1733 In calculating the daily diet it is in most cases sufficient to ascertain how much of the various nutritive bodies must be daily administered to the body to keep it in the proper condition to per- form the work required of it. In other cases it may be required to improve the nutritive condition of the body by properly selected food ; but we also have cases in which we desire to diminish the mass or weight of the body by an insufficient nutrition. This is especially the case in obesity, and all the dietaries proposed for this purpose are chiefly starvation cures. The oldest and most generally-known diet cure for corpulency is that of HARVEY, which is ordinarily called the BANTING method. The principle of this cure consists in increasing, as far as possible, the consumption of the accumulated fat of the body by as limited a supply of fat and carbohydrates as possible and a simultaneous increased supply of proteids. A second cure, called EBSTEIN'S cure, is based on the assumption (not correct) that the fat of the food is not accumulated in a body rich in fat, but is completely burnt. In this cure large quantities of fat are therefore allowed in the food, while the quantity of carbohydrates is diminished very much. The third cure, called OERTEI/S cure, is based on the cor- rect view that a certain quantity of carbohydrates has no greater influence in the accumulation of fat than the isodynamic quantities of fat. In this cure, therefore, carbohydrates as well as fat are allowed, provided the total quantity of the same is not so great as to hinder the decrease in the fatty condition. A greatly dimin- FOOD NEEDED BY MAN UNDER VARIOUS CONDITIONS. 481 ished supply of water is also one of the features of OERTEI/S cure, especially in certain cases. The average amount of the various nutritive substances supplied to the body in these three cures is as follows, arid we give also for comparison in the same table VOIT'S diet necessary for a laborer. TABLE XXII. Proteids. Fat. Carb. Calories. HARVEY-BANTING'S cure... 171 8 75 1066 EBSTEIN'S cure 102 85 47 1391 OERTEL'S " 156 22 72 1124 " (max.) 170 44 114 1557 Laborer, according to VOIT. 118 56 500 2810 If the fat in all cases is recalculated in starch, then the pro- portion of the proteids to the carbohydrates is : HARVEY-BANTING cure 100 : 54 EBSTEIN'S cure 100 : 246 OERTEL'S " 100: 80 " (max.) 100:129 Laborer 100:540 In all these cures for corpulence the quantity of non-nitrogen - ized bodies is diminished as compared to the proteids; but chiefly the total quantity of food, as is shown by the number of calories, is considerably diminished. HARVEY-BANTING'S cure differs from the others in a relatively very much greater amount of proteids, while the total number of calories in it is the smallest. On this account this cure acts very quickly; but it is therefore also more dangerous and more difficult to accomplish. In this regard EBSTEIN'S and OERTEL'S cures (espe- cially OERTEL'S), having a greater variation in the selection of food, are better. As the adipose tissue has a proteid-sparing action, we have to consider in using these cures, especially BANTING'S, that the destruction of proteids in the body is not increased with the decrease in the adipose tissue, and one must therefore carefully watch the elimination of nitrogen by the urine. All diet cures for obesity are moreover, as above stated, starvation cures ; and if the daily amount of food required by an adult man, represented as cal- ories, is in round numbers 2500 calories (according to the average PHYSIOLOGICAL CHEMISTRY. found by FOKSTER in the cue of a physician), then one see what a considerable part of its own mas the body daily give up in the above1 cures. This reminds us of the care necessary in employing these cures ; but each special case should be conducted with regard to the individuality, the weight of the body, the elimination of nitrogen in the urine, etc., etc., and always under strong control and only by physicians, never by a layman. A closer discussion of the many conditions which must be considered in these cane does not enter into the plan and scope of this work. ANIMAL FOODS. TABLE I.— FOODS.1 483 1. Animal Foods. 1000 Parts contain Relationship of 1 Jj ll 2 1 3 !! 4 1 5 1 6 £. 1 :2 :3 a. FLESH WITHOUT BONKS. Fat beef 2 183 196 190 218 190 318 255 100 233 195 253 246 156 167 175 190 135 160 100 120 200 89 121 128 145 100 86 166 98 120 115 80 65 365 660 11 93 14 31 141 83 93 100 332 160 460 540 300 220 67 39 14 2 1 11 18 18 117 13 125 100 40 12 11 14 12 9 15 85 100 8 10 5 60 70 6 10 11 11 8 8 640 688 672 550 717 492 280 130 744 701 719 711 544 585 480 430 437 520 365 200 340 352 469 489 580 440 455 150 150 167 180 88 150 70 80 90 333 333 333 250 450 450 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 90 50 63 53 42 20 143 660 5 48 6 13 90 49 53 53 246 100 460 450 150 246 56 31 9 2 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Beef (average fat 3) Beef * Corned beef (average fat) Veal Horse salted and smoked " " chicken « « " partridge " " wild duck 6. FLESH WITH BONES. Fat beef3 Beef slightly corned. Beef thoroughly corned Pork fresh fat Pork corned fat Smoked ham c. FISHES. River eel, fresh, entire Salmon, ' « Anchovy, ' ' Flounder, ' ' River perch, ' ' Torsk, « ' . 1 The results in the following tables are chiefly compiled from the summary of ALM£N and of K8NIG. As " waste " we here designate that part of the foods which is lost in the preparation of the food or that which is not used by the body; for instance, the bones, skin, egg-shell, and the cellulose in the vegetable foods. * Meat such as is ordinarily sold in the markets in Sweden. • Beef such as is delivered by large purveyors to public institutions in Sweden. 4 Pork, chiefly from the breast and belly, such as occurs in the rations of Swedish soldiers. 484 PHYSIOLOGICAL CHEMISTRY. TABLE I.— FOODS.— (Continued.} Animal Foods. 1000 Parts contain Relationship of !l 11 II a «i £ 3 f| 4 \ 5 1 6 j I 1 :2 :3 82 140 116 200 246 532 665 736 116 196 184 163 221 150 182 190 220 7 3 304 35 35 41 37 230 334 89 106 122 160 103 i 140 43 108 4 5 10 7 103 56 92 106 38 170 2 150 160 850 990 35 7 9 257 270 66 70 93 107 307 7 11 7 50 50 38 35 40 50 456 4 5 7 6 100 107 132 178 106 59 87 11 17 10 10 13 10 9 50 55 15 175 7 7 7 6 60 50 56 8 10 13 8 461 280 334 460 472 257 116 170 770 720 714 721 728 670 807 610 565 119 7 217 873 901 905 665 400 500 329 654 756 520 875 450 340 400 100 100 100 150 135 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 1 100 37 54 1 1 1 1 89 28 50 65 17 113 1 79 73 12100 33000 100 20 22 695 117 19 79 88 88 192 7 0 0 0 0 0 0 0 0 0 6 0 0 0 0 0 0 0 100 0 143 143 93 95 17 15 512 4 4 0 7 Herring salted entire Salmon (side) salted Kabeljau (salted haddock) Codfish (dried ling) " (dried torsk) Fish-meal from variety of GADUS d. INNER ORGANS (FRESH). Brain Beef -liver Heart and lungs of mutton Veal-kidney Ox-tongue (fresh) Blood from various animals (average results) e. OTHER ANIMAL FOODS. Kind of pork-sausage (Mett- wurst) Same for frying • Butter Meat extract Cow's milk (full) Buttermilk Cream Cheese (fat) " (Door) Whey cheese (poor) " '* without shell . Yolk of egg White" " VEGETABLE FOODS TABLE I.— FOODS.— (Continued.) 485 2, Vegetable Foods. 1000 Parts contain Relationship of JL| 00:3 £M £K 2 1 17 10 11 39 10 3 17 15 20 10 14 21 10 60 60 58 7 21 15 15 2 2 2 4 2 1 5 3 1 1 4 25 537 480 fi 4 n 5 1 6 I * 1 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 :2 :3 123 110 92 150 88 90 115 115 114 77 80 111 110 117 140 101 70 232 220 270 20 14 10 25 19 27 31 14 10 12 32 219 4 5 242 140 676 740 768 439 550 768 688 720 725 480 514 654 720 563 660 656 770 537 530 520 200 74 90 50 49 66 33 22 23 38 60 412 130 90 72 180 18 8 3 50 17 8 18 20 15 16 11 26 7 30 20 17 2 36 25 25 10 7 10 8 12 6 19 10 4 7 9 61 3 6 29 50 140 120 120 130 330 131 140 110 110 400 370 140 146 130 100 140 146 137 150 125 760 893 873 904 900 888 908 944 956 934 877 160 832 849 54 55 26 o 8 6 192 5 22 20 16 17 11 48 7 100 20 28 5 37 60 45 8 10 15 9 18 12 8 7 6 8 18 123 31 50 66 95 14 11 12 26 11 3 15 13 18 14 18 19 9 51 43 57 10 9 7 6" 10 14 20 16 11 4 16 21 10 8 12 12 222 343 549 654 835 292 625 853 600 626 634 623 634 589 654 481 471 662 1100 231 240 192 1030 529 900 200 258 244 106 157 230 317 188 188 3250 1800 30 129 Wheat-flour (fine) . . " (very fine). . Wheat-bran Wheat-bread (fresh) " " (fresh coarse) " (fresh fine) .... Barley (grains) Oat (peeled) Corn Rice (peeled for boiling) French beans Flour from peas Potatoes Turnips Cauliflower Cabbage Spinach Lettuce Cucumbers Radishes Edible mushrooms (average).. . . Same dried in the air (average).. Apples and pears . Various berries Cocoa PHYSIOLOGICAL CHEMISTRY. TABLE II.— MALT LIQUORS. 1000 Parts by Weight contain Water. 1 Carbon Dioxide. Alcohol. i 1 I I 00 Glycerine. 1 Porter 871 0, 54 76 7 13 3 4 Beer (Swedish) 887 28 15 •-"^-i 6 • -^-i' 5 5 " (Swedish export). . . 885 911 9, 32 85 55 7 8 7 10 3 31 2 91 3 2 Lager- beer . . 903 2 40 58 4 7 47 1 5 2 2 Bock-beer 881 2 47 72 6 13 1 7 8 916 8 9,5 59 5 4 2 Swedish " Svagdricka". . 945 22 7 *— •— , 2 --*^.» 3 — 3 TABLE III. -WINE AND OTHER ALCOHOLIC LIQUORS. 1000 Parts by Weight contain 1 4a P "l jj i 03 Acid and Bi- tartrate of Potassium. Glycerine. 1 Carbon Dioxide, Vol. per cent. Bordeaux wine 883 94 23 5 9 0 ft White wine (Rheingau). Champagne Rhine wine (sparkling). Tokay 863 776 801 808 115 90 94 120 23 134 105 72 4 115 87 51 5.0 6.0 6.0 7 o 1.0 1.0 9 o 2.0 1.0 2.0 3 0 | 60-70 Sherry 795 170 35 15 5 0 6 0 5 0 774 164 69 40 4 o 2 0 3 0 791 156 53 33 5 0 3 0 3 0 Marsala 790 164 46 35 5 0 4 0 4 0 Swedish punch 479 263 332 Brandy 460 550 Liqueurs • . . > • • 442-590 260-475 INDEX. Absorption, 227-232 ; importance of the cells for the same, 225, 231, 232 ; ac- tion on the putrefactive processes in the intestines, 220. Absorption-ratio, 81 ; of the blood-color- ing matters, 82. Acetic acid, in the gastric juice, 177 ; in the contents of the stomach, 196 ; pas- sage into the urine, 374, 389. Aceto-acetic acid, 422 ; in urine, 420, 421. Acetone, 421 ; in blood, 114 ; in urine, 420. Acetonuria, 421. Acetylen haemoglobin, 74. Acholia, pigmentary, 158. Achroo-dextrin, 171. Acid albuminate, 14 ; properties and be- havior, 23, 24 ; in pepsin digestion, 182, 183. Acid amides, behavior in the body, 389. Acid rigor, 262. Acids, organic, behavior in the body, 389. Acidity, of the contents of the stomach, 195 ; of the urine, 334. Acrolein, 244 ; test, 244, 246. Actinochrom, 325. Acrylic acid, action on the elimination of uric acid, 351. Acrylic acid di-ureid, 350. ADAMKIEWICZ'S reaction, 20. Adenin, 48; properties and occurrence, 51 ; in urine, 359. Adhesion, importance in the coagula- tion of blood, 89. Adipocere, 248. ^Egagropila, 226. Albumin glands, 167. Albumins, 14; general properties, 22 (see also various albumins). Albumin, detection in the urine, 397 ; quantitative estimation, 399 (see also Protein bodies). Albuminous bodies, general behavior, reactions, splitting products and com- position, 15-21 ; summary, 14, 22-30 (see also various albuminous bodies of the tissues and fluids). Albuminate, 14 ; properties and behav- ior, 23, 24 ; ferruginous albuminute in the spleen, 130. Albuminoids, 14, 34 ; in cartilage, 234. 236 ; in the yolk membrane, 291. Albumoses, 14 ; general properties, 25- 30 ; in the putrefaction of proteids, 216 ; in pepsin digestion, 182 ; in tryp. sin digestion, 206 ; in urine, 398 ; nu- tritive value, 460. Alcapton, 365. 487 488 INDEX. Alcohol, see Ethyl-alcohol. Alcoholic fermentation, 7, 307, 409. Alanin, 39. Aleurone crystals, 292^ Alizarin, in the uriue, 393. Alizarin blue, behavior in the tissues, 5. Alkali albuminate, 14 ; properties, 23, 24 ; in the eye, 280, 283 ; in the yolk of the egg, 293 ; in the brain, 273 ; in the non-striated muscles, 272 ; Lieber- kuhn's alkali albuminate, 23. Alkali carbonate, physiological impor- tance, 452 ; action on the secretion of gastric juice, 176 ; occurrence, see the various tissues and fluids. Alkali earths, in the urine, 384 ; in the bones, 238, 239; insufficient supply, 241. Alkali phosphates, in urine, 384 ; occur- rence, see the various tissues. Alkali urates, in sediments, 428 ; in cal- culi, 431. Alkaline fermentation of the urine, 374, 427. Alkaloids, action on the muscles, 262 ; passage into the urine, 393. Allantoic fluid, 358. Allantoin, properties, occurrence, etc., 358 ; formation from uric acid, 350 ; in transudations, 122, 124. Alloxan, 350. ALMEN-BOTTGER'S bismuth test, 409, 411. ALMEN'S guaiacum test for blood, 402. Amanitin, 44. Ambergris, 227. Am brain, 227. Amido-acids, relation to the formation of urea, 338 ; to the formation of uric acid, 352; production in putrefaction, 216 ; in pepsin digestion, 182 ; from protein bodies, 15, 16. 35, 36, 206, 216 ; in trypsin digestion, 206. Amido-acetic acid, see Glycocoll. Amido-acrolein, 37. Amido-caproic acid, see Leucin. Amido-ciunamic acid, behavior in the organism, 391. Amido-ethylsulphuric acid, see Taurin. Amido-phenylacetic acid, behavior in the organism, 392. Amido-phenylpropionic acid, forma- tion in the putrefaction of proteids, 360 ; behavior in the organism, 391, 392. Amido-succinic acid, see Aspartic acid. Ammonia, formation in the putrefac- tion of proteids, 216 ; from proteid substances, 16, 17, 35, 36, 206, 216; in trypsin digestion, 206; in the blood, 114 ; in the urine, 383. Ammonia, elimination in disease of the liver, 383 ; after the administration of mineral acids, 333 ; after extirpation of the liver, 352. Ammonia, estimation in the urine, 384. Ammonia salts, relation to the forma- tion of urea, 338 ; to the formation of uric acid, 352. Ammonio-magnesium phosphate, in urinary calculi, 432 ; in urinary sedi- ments, 430. Ammonium urate, in urinary calculi, 431 ; in urinary sediments, 428. Amniotic fluid, 298. Amphicreatin, 258. Amphopeptone, 26.. Amyl-alcohol, 390. Amyl nitrite, poisoning, 115, 407. Amyloid, 14, 39. Anaemia, 111, 113; malignant, 112. Anhydride theory of the formation of ,glycogen, 140. Aniline, behavior in the organism, 391. Anisotropous substance, 251. Anted on in, 325. Anthrax spores, behavior with gastric juice, 192. Antialbumose, 26. Autipeptoue, 26. INDEX. 489 Antimony, passage into milk, 320 ; ac- tion on the elimination of nitrogen, 337. Autipyrin, action on the urine, 394. Apatite, 238. Arachidic acid, in butter, 303. Arachnoid membrane, fluid of, 122. Arbutin, importance in the formation of glycogen, 139 ; behavior in the or- ganism, 365. Aromatic combinations, behavior in the organism, 390-394. Arsenic, passage into milk, 320 ; into sweat, 328 ; action on the elimination of nitrogen, 337. Arsenic acid, action on pepsin diges- tion, 181. Arseniuretted hydrogen, poisoning, 160-163, 401. Arterin, 68. Ascitic fluids, 124. Asparagin, importance in the synthesis of proteids, 17 ; nutritive value, 460. Asparaginic acid, see Aspartic acid. Asparagus, in the urine, 394. Aspartic acid, relation to the formation of urea, 338; to the formation of uric acid, 352 ; formation from proteids, 17, 206, 210 ; behavior in the organ- ism, 389. Ass's milk, 312. Atmid-albumin, 27. Atmid-albumose, 27. Atropin, action on the secretion of saliva, 173. Auto-intoxication, 12. Bacterium urese, 427. BANTING'S diet cure, 480, 481. Bases, nitrogenized from albumin, 17. Beeswax, 250. Benzoar stone, 226. Benzoic acid, formation from protein substances, 17, 37, 359 ; passage into the sweat, 328; behavior in the or- ganism, 2, 860, 392; occurrence in the urine, 362 ; action on the exchange of material, 466 ; substituted benzoic acids, behavior on the body, 392. Benzol, behavior in the organism, 390, 391. Benzoyl amido-acetic acid, see Hippu- ric acid. Benzoyl chloride, behavior with car- bohydrates, 410, to cystin ; 436. Benzoyl cystin, 426. Benzyl alcohol, behavior in the or- ganism, 3. Bile, 143-163 ; general chemical behav- ior, 144 ; analyses of the same, 157, 158 ; antiseptic action of, 220, 221 ; constituents, 144-156; in disease, 158 ; diastatic action of, 212 ; action on the digestion of proteids, 214, 215 ; on the emulsification of fats, 213, 214; on the secretion of bile, 144 ; on the absorption of fat, 213, 220, 232; on the splitting of neutral fats, 214 ; on trypsin digestion, 206, 215 ; quantity, 143 ; passage of foreign substances into, 158 ; occurrence in the urine, 404, 405 ; in the contents of the stomach, 193 ; in meconium, 224 ; composition, 157. Bile-acids, 146-150 ; hi blood, 114, 159 ; pus, 130; in urine, 162, 228, 404; absorption, 228. Bile-acids, PETTENKOFER'S test for,146. Bile-formation, 159-163. Bile-pigments, 152-156 ; origin, 160, 161 ; reactions, 154, 405, 406 ; pas- sage into urine, 162, 405, 406 ; occur- rence in blood-serum, 63, 114 ; in egg shells, 296. Bile-salts, 145, 146. Bile-secretion, 143. Bilianic acid, 148. Biliary calculi, 163. Biliary fistula, 143 ; influence on putre- faction in the intestines, 220. 490 INDEX. Bilicyanin, 152, 154, 156. Bilifulvin, 153. Bilifuscin, 152, 156, 163. Bilihumin, 152, 156. Biliphaein, 153. Biliprasin, 152, 156. Bilirubin, relation to the blood-coloring matters, 160, 161 ; to haematoidin, 80, 153, 160 ; properties and occurrence, 153 ; in urine, 405 ; in the placenta, 298. Bilirubin calcium, 153, 163. Biliverdin, properties and occurrence. 155 ; in the egg-shell, 296 ; in excre- ments, 223 ; in urine, 405 ; in the placenta, 298. Bitch's milk, 312. Biuret, 339. Biuret reaction, 20, 340. Blister-fluid, 126. Blood, 54-116 ; general behavior, 54, 86, 87 ; analyses, quantitative, 105- 107 ; arterial and venous, 68, 87, 94, 95 ; defibrinated, 55 ; suffocation- blood, 5, 68, 87, 95 ; quantity in body, 115 ; detection, medico-legal, 80 ; behavior in starvation, 110, 448 ; composition under pathological con- ditions, 111-115 ; under physiologi- cal conditions, 108-111; in urine, 401, 402 ; in contents of the stomach, 193. Blood-clot, 55. Blood, coagulation of, 54, 59, 87-94. Blood-coloring matters, 68-83 ; in the urine, 401. Blood-corpuscles, red, 66, 67 ; in urine, 401 ; composition, 83, 106, 112 ; col- orless, 84 ; behavior in the coagula- tion of the blood, 84, 90, 91. Blood-cylinders, 402. Blood, distribution of, in the body, 116. Blood, gases of, 94-105. Blood-loss, 115. Blood-plasma, 54-62 ; composition, 64, 107. Blood-serum, 55, 62-66 ; composition, 64, 106. Blood-spots, 80. Blood-sweat, 328. Blood-tablets, 54, 84, 85 ; importance in coagulation, 91. Blood- transfusion, 111, 115. Blonds, milk of, 316. Blueberry, coloring matter of, in urine, 394. Blue stentorin, 325. BSTTCHER'S spermine crystals, 286. BSTTGEK-ALMEN'S bismuth test, 409, 411. Bones and bone structure, 238-242. Bone earths, 238, 239. Bone, softening of, 340. Bonellin, 325« Borneol, behavior in the organism, 393. Borax, action on the exchange of mate- rial, 466 ; on trypsin digestion, 206. BOWMAN'S disks, 251. Brain, 273-280. Bread, behavior in the stomach, 187 : excrements after feeding on bread, 220. Bromauil, 17. Bromine combinations, passage into the saliva, 173. Bromoform, 17. Brunner's glands, 197. Brunette, milk of, 316. BrussB mucosse, contents of, 127. Buccal mucus, 169. Bufidin, 326. Butter-fat, 303. Buttermilk, 312. Butyric-acid, in contents of the stom- ach, 193, 196 ; in the gastric juice, 177 ; in butter-fat, 303. Butyric-acid fermentation, 4, 409; in intestines, 218. Butyl-alcohol, behavior in the body, 390. INDEX. 491 Butyl-chloral, behavior in the body, 390. Byssus, 14, 39. Cadaverin, 376, 425. Caffein, 48 ; iDfluence on the elimination of uric acid, 352 ; action on the mus- cles, 262. Cairin, action on the urine, 394. Calcium carbonate, in urine, 332 ; in urinary calculi, 432 ; in urinary sedi- ments, 429 ; in bones, 238, 239 ; in tartar, 174. Calcium, lack of, in the food, 241, 453 ; occurrence, see various fluids and tis- sues. Calcium oxalate, 357 ; in urinary sedi- ments, 428 ; in urinary calculi, 432. Calcium phosphate, relationship to the coagulation of casein, 305 ; to fibrin coagulation, 97 ; occurrence in intes- tinal calculi, 226 ; in urine, 332, 384 ; in urinary sediment, 429 ; in urinary calculi, 432 ; in protein bodies, 13, 97 ; in salivary calculi, 174. Calcium sulphate, in urinary sediments, 429. Calories of the food, 471-473 ; various diateries, 474-482. Campherol, 393. Camphor, 375, 393. Camphor-glycuronic acid, 375, 393. Cane-sugar, behavior with intestinal juice, 198 ; with gastric juice, 183. Capric acid, 303. Caproic acid, formation from phenol, 6, 390 ; in fatty tissues, 243 ; in milk- fat, 303. Caprylic acid, 303. Carbamic acid in the blood, 63. Carbohydrates, importance for the for- mation of fat, 249, 463; for the gly- cogen formation, 139 ; for muscular activity, 265, 268, 269 ; exclusive feed- ing on carbohydrates, 249 ; action on the metabolism of proteids, 462 ; on putrefaction, 220 ; absorption, 227, 229, 407 ; inadequate supply of, 454 (see various carbohydrates). Carbolic acid, action on pepsin diges- tion, 181 (see Phenol). Carbolic urine, 365. Carbon dioxide in the blood, 95, 96 ; in diabetes, 104 ; in poisoning with mineral acids, 176 ; in the intestines, 216 ; in the lymph, 118 ; in the con- tents of the stomach, 189; in the muscles in activity and at rest, 264, 265, 268, 269 ; in rigor, 263 ; in secre- tions, 158, 170, 197, 311, 385; in trans- udations, 122 ; binding of CO2 in the blood, 96-100; action on the elimina- tion of gastric juice, 176; tension in blood, 102, 104; tissues, 104; lymph, 118; transudation, 121, 122. Carbon-monoxide blood test, HOPPE- SEYL-ER'S, 73. Carbon dioxide, elimination, depend- ence of the external temperature, 469; in activity and rest, 264, 269, 467 ; by the skin, 329 ; elimination in sleep and waking, 468 ; in various ages, 470. Carbon-dioxide haemoglobin, 74, 97. Carbon-monoxide haemoglobin, 73, 76. Carbon-monoxide methaemoglobin, 75. Carbon-monoxide poisoning, 73, 114 ; action on the elimination of nitrogen, 337 ; on the elimination of sugar, 407. Carminic acid, 325. Cam in, 48 ; properties, 258 ; in urine, 359. Carp, sperma of, 287. Cartilage, 234-238 ; behavior with gas- tric juice, 183, 187 ; with pancreatic juice, 211. Cartilage glue, 39, 234, 238. Casein, origin, 300, 318, 319; from woman's milk, 313 ; from cow's milk, 304 ; quantitative estimation, 309 ; re- lationship to rennet, 304, 305 ; to gas- tric juice, 313. 492 INDEX. Caseoses, 27. Castoreum, 326. Castorin, 326. Cataract, 283. Cat's milk, 312. Cell, animal, 41-53 ; nucleus, 46 ; mem- brane, 43, 183. Cell-globulin, 58. Cellulose, marsh-gas fermentation of, 213, 219; behavior in the intestine, 213. Cement, 241. Cephaline, 274. Cerebrin, 129, 274 ; properties, 275-278. Cerebro-spinal fluid, 125. Cerolein, 250. Cerotinic acid, 250. Cerumen, 326. Cetiu, 250. Cetyl-alcohol, 250. Cetylid, 276. Chalaza, 294. Clmrcot's crystals, 113, 286. Cheese, 302, 305. Chenotaurocholic acid, 148. Chitin, 323 ; behavior in trypsin diges- tion, 211. Chloral hydrate, behavior in the body, 375, 390. Chlorate, poisoning with, 115, 401. Chlorazol, 17. Chlorbenzol, behavior in the body, 393. Chlorides, elimination by the urine, 65, 377 ; by the sweat, 828 ; insufficient supply, 451 ; action on the metabolism of proteids, 377, 466 (see the various fluids and tissues). Chlorocruorin, 83. Chloroform, action on the elimination of chlorine, 377 ; on the muscles, 262. Chlorophan, 282. Chlorophenyl-cystein, 393. Chlorphenyl-mercapturic acid, 393. Chlorophyll, 325. Chlorosis, 112, 113. Chlorrhodinic acid, 130. Cholalic acid, 148 ; relation to choles- terin, 164. Cholanic acid, 150. Cholecyanin, 154. Choleglobin, 162. Choleic acid, 147, 149. Cholera bacillus, behavior with gastric juice, 192. Cholera, blood, 111, 112, 113, 114; con- tents of the stomach, 225; sweat, 328. Cholesterin, general chemical proper- ties and occurrence, 164 ; in gall- stones, 164 ; in the brain, 274, 279 ; in the urine, 424 ; importance for life- processes in the cell, 53. Cholesterin stones, 164. Cholesterilin, 164. Cholestrone, 164. Choletelin, 152, 155; relation to uro- bilin, 370. Cholic acid, 146, 148. Cholin, 44, 156. Cholohaematin, 156. Choloidic acid, 150, 151. Chondrigen, 37, 234. Chondrin, 39, 234; in pus, 130. Chondrin balls, 236. Chondroitic acid, 235, 236. Chondromucoid, 34, 234. Chorda saliva, 168. Chorioidea, 283 ; pigment, 324. CHRISTENSEN & MYGGK, approximative estimation of albumin in the urine, 400. Chromidrosis, 328. Chromogens, in the urine, 369 ; in the suprarenal body, 134. Chrysophanic acid, action on the urine, 393. Chyle, 117, 118. Chylopericardium, 123. Chyluria, 424. Chyme, 186; investigation of, 193-196. Chymosiu, 184. INDEX. Cinnamic acid, behavior in the body, 1 Corpora lutea, 288. 493 360. Citric acid, in milk, 304, 811. Coagulation of blood, 54, 59, 87-94; of milk, 187, 301, 302, 305; of muscle- plasma, 252, 254, 255. Coccygeal glands, secretion of, 326. Cochineal, 325. Co3fficient, HASER'S, 387. Coffee, action on the exchange of ma- terial, 467. Collagen, 14, 37, 38; in connective tis- sues, 233; in the cornea, 237; in carti- lage, 234, 236. Colloid, 34, 289. Colloid corpuscles, 289. Colloid cysts, 289. Coloring matters, of the eye, 280; of the blood, 68-83; of blood-serum, 63; of the corpora lutea, 288; of the egg- shell, 296; of the fat-cells, 243; of the bile, 145, 152, 156, 160, 161; of the urine, 369-374; of the skin, 324, 325; of the lobster, 325 ; of bird -feathers, 325; medicinal coloring matters in the urine, 406. Colostrum, of woman's milk, 315; of cow's milk, 311. Colostrum corpuscles, 302, 311, 319. Combustion, heat of, of foods, 471-473. Comma bacillus, behavior in gastric juice, 192. Conchiolin, 14, 39. Concrements, see various calculi. Cones of the retina, pigments of, 282. Conglutin, 472. Cornikrystalline, 39. Connective tissue, 233. Copaiva balsam, action on the urine, 394. Copper, in blood, 107; in haemocyanin, 83; in the liver, 137; in protein sub- stances, 13. Cornea, 237, 283. Cornein, 14, 39. Jorpsewax, 248. yorpuleuce, diet cures, 480-482. Cow's milk, 301-312; general behavior, 301, 302; analysis of, 308-310; con- stituents, in organic, 310; organic, 304-307 ; coagulation with rennet. 302, 304; in stomach, 187; composi- tion, 310-312. Cream, 312. Creatin, relationship to the formation of urea, 257, 339; to muscular activ- ity, 266, 268; properties and oc- currence, 257; behavior in the organ- ism, 389. Creatiniu, relationship to muscular ac- tivity, 266, 268, 347 ; properties and occurrence, 347; creatinin zinc chlo- ride, 348. Cresol, 363. Cresol -sulphuric acid, 363, 364. Crotonic acid, 423. Cruor, 55. Cruso-creatinin, 258. Crustaceorubin, 325. Crusta iuflammatoria or phlogistica, 87, 113. Cumic acid, 392. Cuminuric acid, 393. Curara poisoning, action on the muscles, 264 ; on the elimination of sugar, 407. Crystalbumin, 283. Crystalfibrin, 283. Crystallin, 282. Crystalline lens, 282. Cyanocrystallin , 296, 325. Cynogen, in the proteid molecule, 4. Cyanuric acid, 350. Cyanurin, 370. Cymol. behavior in the body, 392. Cynurenic acid, 377. Cystein, 425; grouping in the body, 393, 425. Cystin properties and occurrence, 425 ; in the urine, 376; in urinary sediment, 494 INDEX. 430 ; in urinary calculi, 432 ; in the liver, 137 ; in the kidneys, 376 ; in sweat, 328. Cysts, tapeworm, 126; thyroid, 133; ovarial, 288-291. Cystinuria, 376. Daraaluric acid, 376. Damolic acid, 376. Delomorphic cell, 175. Density method of determining albu- min, 401. Dentin, 239, 241. Dehydrocholalic acid, 148. Dehydrocholeic acid, 150. Desoxycholalic acid, 148. Deutero-albumose, 27. Dextrin, formation from starch, 171, 202 ; occurrence in the contents of the stomach, 187, in the muscle, 260. Dextrin, substance similar to, in urine, 374. Dextrose, see Grape-sugar. Diabetes mellitus, elimination of am- monia by the urine, 384; relationship of the liver, 142, and the pancreas, 407, to the elimination of sugar ; blood, fat, 114; amount of sugar, 63, 114; urine, general behavior, 332, 387, 407; amount of sugar, 407; amount of CO2 of the blood, 104; oxybutyric acid in blood, 104 ; in the urine, 423 ; relation to the crystalline lens, 283. Diabetic sugar, see Grape-sugar. Diacetic acid, see Aceto- acetic acid. Diamine, in the urine, 376, 425 ; in the contents of the intestines, 425. Diastatic enzyme, see Enzymes. Diazo-oxyacrylic acid ester, 37. Diet for various classes of people, 474- 482. Diet cures for obesity, 480-482. Digestion, 167-232. Digestibility of various foods, 189, 190, 191, 476. Dimethyl carbinol, behavior in the organism, 390. Dimethyl-ketoue, see Acetone. Dioxybenzol, 391. Dioxynaphthalin, 391. Distearyl-lecithin, 43. Doglingic acid, 246. DONNE'S pus test, 404. DOREMUS'S urea determination, 346. Dotterplattchen, 17, 292, 296. Dropsy, 112, 113. Dys-albumose, 27. Dyslysin, 150. Dys-peptone, 182. Dyspnoea, action on the metabolism of proteids, 337, 468. Earthy phosphates, elimination by the urine, 379, 384, 448 ; solubility in al- buminous liquids, 241 ; occurrence in bone-earths, 238, 239 ; in concre- ments, 163, 174, 226, 431, 432; in sedi- ments, 429, 430 (see various earthy phosphates). EBSTEIN'S diet cure, 480. Echinochrom, 83. Eel, blood-serum, 64; flesh, 271. Egg, 287; hen's egg, 291-298; hatching of, 297. Egg-albumin, see Ovalbumin. Egg-globulin, 295. Egg, yellow, 291. EHRLICH'S bile-pigment test, 406 EISELT'S reaction, 403. Elaic acid, 245. Elaidic acid, 246. Elaidin, 246. Elastin, 14, 36 ; behavior with gastric juice, 183 ; with trypsin, 21 J Elastinalbumose, 36. Elastinpetone, 36. Ellagicacid, 227. Emydin, 296. Enamel, of teeth, 242. Encephaliu, 276, 277. INDEX. Endolymph, 284. Energy, potential, of foods, 471. Enzymes, general, 8, 9, 10 ; pancreatic diastase, 201; in blood, 142; bile, 210; urine, 376 ; muscle, 256 ; in secre- tions of the mucous coat of intes- tines, 197; in saliva, 171; enzymes m the mucous coat of the intestines which dissolve proteids, 197 ; in urine, 376; in stomach, 178; in lower animals, 178; in pancreas, 201, 204; in the plant kingdom, 178 ; fat-split- ting enzymes, 202, 203 ; enzymes pro- ducing coagulation, see Fibrin, Ferment and Rennet ; urea-splitting enzyme, 427. Erythrodextriu, 171. Erythropsin, see Visual purple. ESBACH'S albumin determination, 400 ; urea determination, 346. Euxanthonic acid, 375. Ethal, 250. Ether, action on blood, 67 ; on the se- cretion of gastric juice, 176; on the muscles, 262; on the secretion of pan- creatic juice, 201. Ethereal oils, action on the muscles, 262. Ethereal sulphuric acids, in the urine, 216, 363, 391, 392; in sweat, 327. Ethidine lactic acid, 260. Ethyl-alcohol, passage of, into milk, 320; behavior in the organism, 466 ; action on the secretion of gastric juice, 176 ; on muscles, 262 ; on di- gestion, 182, 189. Ethylen lactic acid, 260. Ethylenimin, 286. Exchange of material, 435-471. Excrements, 219, 222-224; in dogs with biliary fistula, 221 ; in starva- tion, 439 ; with various foods, 222 ; elimination of water with the excre- ments, 438. Excretin, 223. Excretolic acid, 223. Exostose, 241. Extinction coefficient, 81. Exudations, 117, 121. Eye, 280-283. Faeces, see Excrements. Fats, origin in the body, 247 ; general properties, detection, and occurrence, 242-247: emulsification of fats, 198, 202, 203, 213, 214, 227, 244; fat in blood-serum, 62, 111, 113; chyle, 118 ; yolk of egg, 293 ; pus, r29; fatty tissues, 242, 243 ; bile, 156 ; brain, 274 ; urine, 424 ; bones, 239 ; milk, 302, 303, 310, 313, 317, 318 ; nutri- tive value, 460, 461, 471-473; ab- sorption, 227, 232 ; heat of combus- tion, 471-473 ; behavior to the intes- tinal juice, 198 ; to gastric juice, 183, 188 ; to pancreatic juice, 203 ; saponi- fication of, 203, 213, 214 ; action on the secretion of bile, 144. Fat, metabolism in activity and at rest, 268, 269; in starvation, 445, with various foods, 456, 461-466, 480-482. Fat cells, 183, 211, 242. Fat sweat, 326. Fatty acids, general properties, detec- tion, and occurrence, 243-247 ; feed- ing with, 247 ; nutritive value, 461 ; syntheses to neutral fats, 248. Fatty series, behavior in the organism, 388. Fatty tissues, 242, 243; behavior with gastric juice, 183, 188. Feathers, 34, 325. FEHLING'S solution, 415. Fellic acid, 150. Ferments, general, 8 (see various fer- ' meuts). Fermentation, 4, 8 ; of the urine, 374, 427 ; of the contents of the stomach, 187, 188, 193 (see also various fer- mentations, such as the alcoholic, butyric fermentations). 496 INDEX. Fermentation lactic acid, properties, oc- currence, etc., 260, 261; in brain, 274 ; in the contents of the stomach, 188 ; gastric juice, 177 ; production in the souring of milk, 301 ; in the fermentation of urine, 427; detection in the contents of the stomach, 194. Fermentation test, on the urine, 412. Fevers, elimination of ammonia, 384 ; uric acid, 352 ; urea, 337 ; potassium salts, 383 ; blood in fevers, 113, 114; metabolism of proteids in, 337. Fibrin, 14, 55; properties, 57; occur- rence in transudations, 117, 121, 123; Henle's fibrin, 285. Fibrin coagulation, 58, 89-94. Fibrin concrements, 226. Fibrin ferment, 55, 58, 85, 89-93, 117. Fibrin globulin, 59, 62. Fibrine, soluble, see Serum-globulin. Fibrinogen, 14, 56, 62, 92, 117. Fibrinoplastic substance, see Serum globulin. Fibroin, 14, 39. Fish, egg, 18, 296 ; bones, 240 ; scales, 50 ; air-bladder, 50. Flesh, amount of nitrogen in, 272, 441; digestibility, 187, 190 ; composition, 270, 271 (see also Muscle). Flesh, metabolism of, in starvation, 445 ; in various foods, 454-465. Flesh accumulation of, with various foods, 454, 455, 462, 463. Fluorine, in bones, 238, 239; in enamel, 242. Fly-maggots, formation of fat in, 248. Formic acid, in butter, 303 ; in con- tents of the stomach, 196 ; passage into the urine, 374. Frog's eggs, membrane of the same, 32. Fumaric acid, 17. Fundus glands, 175, 185. Furfurol, relation to PETTENKOFER'S test for bile acids, 146 ; behavior in the body, 389. Furfuracrylic acid, 389. FURBRLNGER'S albumin reagent, 397. Fuscin, 282. Galactose, 306; from cerebrin, 377; from vegetable bodies, 320. GALLOIS'S inosit test, 259. Gases of the blood, 94-105; of the con- tents of the intestine, 218 ; of the bile, 158 ; of the urine, 385 ; of the hen's egg, 296 ; of tbe cbyle, 118 ; of milk, 311 ; of muscles, 262, 264 ; of transu- dations, 112. Gas, exchange of, with various ages, 470 ; by the skin, 328 ; in starvation, 446 ; in various conditions of the body, 467, 468 ; in the muscles, 264 ; with various foods, 447. Gastric juice, 175 ; secretion of, 176 ; degree of acidity, 194 ; artificial gas- tric juice, 179; action, 23, 26, 38, 179-184, 186-192. Gelatine, 38 ; importance in the forma- tion of glycogen, 139 ; putrefaction, 37, 216; nutritive value, 459; behavior with gastric juice, 183, 211 ; to pan- creatic juice, 211. Gelatine, forming tissues, see Collogen. Gelatine peptone, 38. Generative organs, 285-299. Germ, of the egg, 291. Globulins, 14 ; general character, 22 ; in the urine, 397, 400; in the proto- plasm, 42 (see various globulins). Globulin tablets, 86. Globuloses, 27. Glucosamine, 323. Glucose, formation by the action of saliva, 171 ; of pancreatic juice, 202 (see Grape-sugar). Glue sugar, see Glycocoll. Glutamic acid, 16. Glutin, in pus, 130 (see Glue). Glycerin, importance for the formation of glycogen, 139, 140; relationship to INDEX. 497 the synthesis of fats, 248 ; action on the elimination of uric acid, 351; solvent for enzymes, 9; nutritive value, 462. » Glycero-phosphoric acid, 43, 113, 127, 131, 134, 156; in the urine, 374, 376. Glycin, see Glycocoll. Glycocholic acid, 145 ; properties, 146 ; quantity in excrements, 219 ; in vari- ous animal bile, 158 ; behavior in the putrefaction in the intestines, 219. Glycocoll, properties, 150 ; production in the putrefaction of gelatine, 37, 216 ; from protein substances, 37-39 ; relation to the formation of uric acid, 350, 352; to the formation of urea, 338, 339 ; syntheses with glycocoll, 3, 160, 359, 389, 392. Glycogen, 138-143 ; origin, 139 ; gen- eral chemical behavior, 138, 139; relation to the formation of sugar, 141, 142 ; to muscular activity, 265- 268 ; to rigor mortis, 263; occurrence in the muscles, 250; in Uue protoplasm 42, 52, 85. Glyconic acid, 374. Glycosuria, 63, 142, 407. Glycuronic acid, properties, 374; paired glycuronic acids, 217, 367, 374; grouping in the body, 390, 393 ; origin, 390. Glycuron, 374. Glyoxyldiureid, 358. GMELIN'S test for bile pigments, 154; in the urine, 405. Goat milk, 312. Gout, elimination of uric acid in, 351, 352. GRAAFFIAN vesicles, 287. Grape-sugar, properties and occurrence, 406-409 ; in urine, 63, 142, 406-419 ; detection, 410-414; quantitative esti- mation, 414-419 ; reactions, 408-410. Guaiacum blood test, 402. Guauin, properties and occurrence, 50; in urine, 359 ; quantity in liver, 137; pancreas, 200 ; spleen, 287. Guanin gout, 51. Guanin lime, 50. Guano, 49, 50, 851. Guano biliary acids, 147. Guanovulit, 296. Gum, animal, 32 ; in urine, 374. Hsemin, 78, 79. Haemin crystals, 79, 403. Hsemochromogen, 69 ; properties, 76 ; in muscle, 256. Haemocyanin, 83. Haemoglobin, properties and behavior, 72 ; quantity in blood, 69, 108-111 ; quantitative estimation, 82 ; behavior in the trypsin digestion, 211 (see Oxyhaemoglobin and its combinations with other gases). Haemoglobinuria, 401. Haemometer, 82. Hasmatocrystallin, see Oxyhaemoglo- bin. Hair, 34 ; ash, 322 ; pigments of, 324. 325. Hair-balls, 226. HASER'S coefficient, 387. Haematin, relation to bilirubin, 161 ; to urobilin, 161, 371 ; properties, 76. Hsematinometer, 81. Haematochlorin, 298. Haematogen, 292, 297. Haematoglobulin, see Oxyhaemoglobin Haematoidin, relation to bilirubin, 80, 153, 160, 162 ; properties, 80 ; occur- rence, corp. lutea, 288 ; in excre- ments, 223 ; sediments, 430. Haematolin, 78. Haematoporphyrin, relation to bilirubin, 78, 161 ; to urobilin, 371 ; properties, 78 ; occurrence in lower animals, 825. Haematosiderin, 162. Haematuria, 401. Haemerythrin, 83. 498 INDEX. Heat, action on the exchange of ma- terial, 469; development of heat in plants, 2. HELLER'S albumin test, 19; in urine, 396. HELLER-TEICHMANN'S blood test, 403. Hemi-albumose, 26. Hemi-collin, 38. Hemi-elastin, 36. Hemi-peptone, 26. Hemp-seed stone, 432. Hen's egg, 291-298; hatching, 297. Heteroalbumose, 27. Heteroxanthin, 48, 359. Hippomelanin, 324. Hippuric acid, 359; properties and re- actions, 361; formation in the body, 3, 360, 361, 392; splitting, 360, 362; occurrence, 360; in sediments, 430. Histozyme, 363. HOFMANN'S tyrosin test, 209. Holothuria, Mucin, 34. Homocerebin, 276, 277. HOPPE-SEYLER'S carbon monoxide test, 73; xanthin test, 49. Horn, 35. Horn substance, see Keratin. Horse's milk, 312. Humin substance, 369, 394. Humor, aqueous, 126. HUPPERT'S bile-pigment reaction, 154, 405. Hyaline, 34, 323. Hyaline substance of ROVIDA, 67, 85, 129. Hyalogen, 14, 34. Hydracrylic, 260. Hydraemia, 112. Hydramnion, 299. Hydrobilirubin, 152, 156 ; relation to urobilin, 161, 228, 370 ; formation in putrefaction, 219. Hydrocele fluid, 125. Hydrochinon, 365. 394. Hydrochiuon sulphuric acid, 363, 365. Hydrocinnamic acid, behavior in the body, 360. Hydrochloric acid, secretion by the stomach, 176 ; anti-fermentive ac- tion, 192 ; action on opening the py- lorus, 189 ; on the secretion of pepsin, 177 ; quantity of, in the gastric juice, 177 ; reagents for free HC1, 194, 195 ; action on albumin, 20, 23, 180. Hydrocyanic acid, action on pepsin di- gestion, 181; on trypsin digestion,206. Hydrogen, in putrefactive and fermen- tive processes, 4, 216, 218. Hydrogen peroxide, in urine, 385. Hydrolytic splitting, 8. Hydronephrosis fluid, 331. Hydroparacumaric acid in putrefac- tion in the intestines, 216. Hydrophenoketon, 6. Hydrops folliculorum Graafii, 288. Hyo-glycocholic acid, 147. Hypalbuminose, 113. Hyperalbuminose, 113. Hyperinose, 113. Hypinose, 113. Hyposulphites, in the urine, 376. Hypoxanthin, relation to the formation of uric acid, 352, 389 ; properties, etc., 50 ; quantity in the liver, 137 ; in muscles, 256 ; pancreas, 200 ; semen, 287. Iceterus, 143, 162; blood, 114; in urine, 405. Ichthidin, 296. Ichthin, 296. Ichthulin, 296. Indican, 366-368. Indican elimination in starvation, 219, 366; in disease, 366. Indigo, 366; in sweat, 328. Indigo blue, 367, 370. Indol, properties, 218; formation from proteids, 16, 17; in putrefaction, 216, 217, 363, 366. INDEX. 499 IndopheuoL blue, behavior in the tis- sues, 5. Indoxyl, 216, 366. Indoxyl glucoronic acid, 366, 367, 393. Indoxyl red, 367. ludoxyl sulphuric acid, 363, 366, 367. Inosit, properties and occurrence, 256; in urine, 420. Inosinic acid, 256. Intestine, contents, 212-225. Intestine, putrefaction processes there- in, 215-222; absorption, 215, 216, 225, 227-232; digestive processes, 212-216. Intestine, glands of the mucous mem- brane, 197. Intestinal calculi, 226. Intestinal fistula, 197. Intestinal gases, 218. Intestinal juice, 197, 198. Iodine combinations, passage of, into milk, 320; sweat, 328; saliva, 173. lodoform test, GUNNING'S, 421; LIE- BEN'S, 421. Isclmria, 328. Isodynamic value of foods, 473. Isocholesterin, 165. Isotropous substance, 251. Iron in blood-coloring matters, 70, 76, 77, 81. 161; in blood, 63, 65, 106; in the bile, 157. 161; in urine, 385; in the liver, 136, 137; In milk, 310, 315, 320; in the spleen, 130, 131, 136; in muscles 262; in protein substances, 15, 23, 130, 136; elimination of iron, 157, 181. 174. 385; amount of iron in dog's milk and new-born dogs, 317; absorption. 453. Iron salts, elimination by the urine, 385; action on the blood, 111; on trypsin digestion, 206; absorption. 453. JAFFE'S indican test, 387; creatinin re- action, 349. Janthinin, 325. Jaune indien, 375. Jecorin, properties and occurrence, 137. Kephir, 307. Kerasene, 276. Keratin, 14, 322; properties, 34; in egg-shells, 296; in bones, 238; be-- havior with gastric juice, 183; to pancreatic juice, 211. Keratinose, 35. Kidneys, 331 ; relation to the formation of uric acid, 352; to urea, 339; to hip- puric acid, 361. KJELDAHL'S method of determining ni- trogen, 341, 345. KNAPP'S titration, method, 417. KNOP-HUFNER method for determin- ing urea, 346. Kumyss, 307. Kyestein, 430. Lactalbumin, 14; properties, 306; in human milk, 313. Lactates, 261. Lactic acid, 260 (see Paralactic acid and Fermentation lactic acid). Lactic acid, fermentation, 307, 409; in urine, 427; in stomach, 187, 188; in milk, 301. Lacto- caramel, 307. Lacto-globulin, 306. Lacto-protein, 306. Lactose, 306. Lsevulinic acid, 32. Latebra, 291. Laxatives, action on the blood, 113; on the secretion of intestinal juice, 197; action of, 225. Lead, in blood, 107; in the liver, 137; passage into the milk, 320. Lecithin, properties and occurrence, 43; action on the coagulation of the blood, 92; putrefaction of, 45, 219; relation to muscular activity, 266. Lens, see Crystalline lens. 500 INDEX. Lens capsule, 282. Lethal, 250. Leucaemia, 48, 113 ; elimination of urea, 352 ; xanthin bodies, 48, 113, 132, 359. Leuceines, 16. Leucin, 16, 17, 206; relationship to the formation of uric acid, 352; to the formation of urea, 338, 389 , preparation, 209 ; properties, occur- rence, etc., 206 ; passage into the urine, 424 ; behavior in the body, 338, 389. Leucinimid, 17. Leucocytes, see Colorless blood- corpus- cles. Leucomaines, 12 ; in urine, 376. Levulose in urine, 419. LIEBEKKUHN'S glands, 197. LIEBIG'S titration method for estimating the quantity of urea, 341. Ligamentum nuchae, 36. Lime, lack of, in foods, 241, 453. Linseed oil, feeding with, 247. Lipacidaemia, 114. Lipaemia, 113. Lipochrome, 63, 393. Lipuria, 424. Lithium in blood, 107. Lithobilic acid, 227. Lithofellic acid, 227. Lithuric acid, 377. Liver, 135-137 ; relationship to the formation of uric acid, 352, 353 ; to the formation of urea, 338, 339; blood of the liver, 108, 141; albuminous bodies, 136 ; fat of, 136 ; amount of sugar, 141. Liver, atrophy, acute yellow, secretion of ammonia, 338, 384 ; of urea, 338, 384 ; leucin and tyrosin, 424 ; lactic acid, 260, 374. Liver, cirrhosis, ascitical fluids, 124 ; action on the elimination of ammonia and urea, 338, 384. Liver extirpation, elimination of am- monia, 352 ; of uric acid, 352 ; lactic acid, 352, 373 ; action on the forma- tion of bile, 159, 160. Luteines, 293 ; in corpora lutea, 288 -f in yolk of the egg, 293 ; in serum, 63; relationship tojiaematoidin, 80, 288. Lymph, 117-121? Lymphatic gland, 130. Lymph fibrinogen, 85. Lymph-cells, proteids, 42 see (Color- less blood-corpuscles). Mackerel flesh, 271. Madder in urine, 393. Magnesium in the urine, 384 ; in bones, 238, 239 ; in muscles, 262, 270 (see various tissues and fluids). Magnesium phosphate, in intestinal cal- culi, 226 ; in urine, 393 ; in urinary calculi, 431, 432; in urinary sedi- ments, 430 ; in bones, 238, 239. Malaria, blood in, 113. Malt diastase, 171. Maltose, formation from starch, 171, 202 ; behavior to intestinal juice, 198, 213. Mammary glands, 300, 319, 320. Mandelic acid, 392. Mare's milk, 312. Margarin and margaric acid, 245. Marsh-gas, in intestine, 218, 219 ; in intestinal putrefaction, 216 ; in the fermentation of cellulose, 213, 218 ; by the decomposition of lecithin, 219. Material, exchange of, dependence on the external temperature, 469 ; in various ages, 470 ; in activity and rest, 264-270, 467, 468; in various sexes, 470 ; in starvation, 443-449 ; with various foods, 454-465 ; in sleep and waking, 468 ; calculation of the extent of exchange of material, 441, 442. Meconium, 224. INDEX. 501 Melanin, relation to blood-coloring matters, 162, 324 ; properties, occur- rence, and composition, 324, 325 ; in the eye, 282 ; in the urine, 403. Melanaemia, 114. Melauogen, 403. Melauotic tumor, coloring matters of, 324. Melissyl alcohol, 250. Mellitaemia, 114. Menstrual blood, 109. Menthol, behavior in the body, 393. Mercapturic acid, 393. Mesitylen, behavior in the body, 392. Mesitylenic acid, 392. Mesitylenuric acid, 393. Metalbumin, 289. Metallic salts, action on enzymotic pro- cesses, 172, 181, 206. Metaphosphoric acid, constituents of the nucleins, 23, 46; albumin rea- gent, 19 ; in urine, 396. Methal, 250. Methsemoglobin, 74 ; in blood in pois- oning, 115 ; in urine, 401. Methyl-guanidin, 348. Methyl-guanidin acetic acid, see Crea- lin. Methyl-glycocoll, see Sarkosin. Methyl-hydantoin, 350. Methyl hydantoinic acid, 389. Methyl-indol, see Skatol. Methylpyridyl-ammonium hydroxyl, 393. Methyl-uric acid, 350. Methyl-uramin, 348. Micrococcus urese, 427. Micro-organisms in the intestinal canal, 10, 215. Milk, 300-322; secretion, 318-322; blue or red milk, 321; milk in disease, 320; passage of foreign bodies into, 320 (see various varieties of milk). Milk-fat, 303; analysis, 309; formation, 319. Milk-globules of cow's milk, 302, 303; of human milk, 313. Milk, human, 312-316; behavior in stomach, 187, 313; composition, 314. Milk-plasma, 304. Milk-sugar, properties, 306, 307; fer- mentation, 187, 301, 307; quantita- tive estimation, 308, 310; passage into the urine, 307, 419; origin, 320. Mineral acids, 104, 333, 450, 452; anti- fermentive action, 192; action on the elimination of ammonia, 333, 452. Mineral bodies, elimination in starva- tion, 448; inadequate supply of, 449; behavior in the organism, 450 (see various fluids, tissues, and juices). MILLON'B reagent, 20. MOHB'S titration method for chlorine, 378. MOORE'S sugar test, 408. Morphine, passage into milk, 320; into urine, 393. Mucic acid, 306. Mucin, 14; properties and composition, 32; in connective tissue, 233; in urine, 376, 395; in salivary glands, 32, 168; detection, 401. Mucin-like bodies in bile, 145; urine, 376, 395; kidneys, 331; thyroid gland, 133; synovial fluid, 126. Mucinogen, 32. Mucinoid, 14, 31, 34. Mucoid, 14, 31, 34; in ascitical fluids, 124; cornea, 237; vitreous humor, 234, 282. Mucous tissues, 234. Mucus, of the bile, 144; of the urine, 331, 376, 401; of the synovia, 126. Mulberry calculi, 432. Murexid test, 354. Muscles, non-striated, 272; striated, 251-272; blood of the same, 109, 264, 265, 268; chemical processes in activity and at rest, 264-270; in rigor mortis, 263; albuminous bodies, 252- INDEX. 256; extractives, 256-261; coloring matters, 256; fat, 261, 268, 270, 271; gases, 262, 264-268; mineral bodies, 261, 262, 270; reaction, 251, 252, 266; amount of water, 272; composition, 270. Muscle-coloring matters, 256. Muscle-fibre, 252. Muscle-plasma, 252 ; coagulation of, 252, 254, 263, 272. Muscle-serum, 252. Muscle-sugar, 260. Muscle-stroma, 255. Muscle- syiiton in, 256. Muscular activity, chemical processes in the muscles, 264-270 ; action on the urine, 333, 347; on the exchange of material, 264-270, 467, 468, 478, 479. Musculin, 14 ; properties, 255. Mutton tallow, 247. Myeline, 274. My el in e forms, 274. MYGGE and CHRISTENSEN'S albumin determinate, 400. Myoalbumin, 255. Myoalbumose, 255. Myosin, 252 ; properties, 253, 254 ; in protoplasma, 42, 84. Myosin-ferment, 254. Myosinogen, 254. Myosiuoses, 27. Myoglobulin, 255. Myohaematin, 256. Myricyl alcohol, 250. Myrisin, 250. Myristinic acid in butter, 303. Myxoederma, 133. Myxoid cyst, 288. Nails, 34, 35, 322. Naphthalin, changing of the urine, 394 ; behavior in the body, 394. Napthol, reagent for sugar, 410, 414; behavior in the oody, 394. Naphthol glycuronic acid, 313, 393. Navel-cord, mucin of, 32, 234. Nebecula, 331. Neossin, 34. Nerves, 273. Neuridin, 278, 291. Neurin, 44; in supra-renal body, 134. Neurokeratin, 35, 273, 280. Neutral fats, see Fats. Nitrates in urine, 383. Nitric-oxide haemoglobin, 74. Nitrobenzoic acid, 17. Nitrobenzyl-alcohol, 393. Nitrogen, elimination in rest and activ- ity, 267-270, 467, 468; in starvation, 444, 445 ; with various foods, 454-465; elimination, by the excrement, 440 ; urine, 337, 338, 380, 382, 438, 439, 440 ; horn-formation, 439, 440 ; sweat, 439; relation to the elimination of phos- phoric acid, 380 ; to sulphuric acid, 338. Nitrogen, free, in blood, 94; in intestines, 218; in stomach, 189 ; in secretions, 158, 170, 311, 385; in transudations, 122; combined nitrogen, quantity of, in the discharges of the intestines, 440 ; in meat, 272, 441 ; in urine, 337, 338; determination, 341-347 ; in protein substances, 15, 32, 35, 39. Nitrogen deficit, 439. Nitrogenous equilibrium, 440; with va- rious foods, 454-465. Nitrophenyl-propiolic acid, reagent for sugar, 410, 414 ; behavior in the body, 366, 367. Nitrosoindol-nitrate, 217. Nitrotoluol, behavior in the body, 393. Nitrotyrosin-nitrate, 209. Non-striated muscles, 272. Nuclein, properties and occurrence, 46; in blood- corpuscles, 67; pus, 129 ; brain, 273 ; sperma, 287. Nuclein protamin, 287. Nucleoalbumin, 14; properties, 22; in INDEX. 603 bile, 145; in urine, 376, 396; kidneys, 331 ; mammary glands, 300, 319; sy- novia, 126. Nutritive bodies, necessary, 435 ; heat of combustion of, 471-473. Nutrition, need of man for, 473-480. Nutrition, influence on the secretion of intestinal juice, 197 ; bile, 143, 144 ; gastric juice, 176 ; pancreatic juice, 200. ou the elimination of ammonia, 384 ; uric acid, 351 ; urea, 337, 45< 463, 465 ; carbon dioxide, 447 ; min- eral bodies, 337, 379, 382 ; on the ex- change of material, 449-465 ; various foods rich in proteids, 454-460 ; pro- teid and fat, 460-462 ; proteid and carbohydrates, 462-465 ; insufficient, 449-454. NYLANDER'S test for sugar, 411. Odoriferous bodies in urine, 394. (Edema, fluid of, 126. ORTEL'S diet cure, 480, 481. Oleic acid, 245. Olein, 243-245. Oligmia, 112. Oligocythaemia, 112. OHguria, 387. Olive-oil, action on the secretion of bile, 144. Onuphin, 34. Oocyan, 296. Oorodein, 296. Opium, passage into the milk, 320. Optogram, 282. Ornithin, 389, 392. Ornithuric acid, 392. Organs, loss of weight in starvation, 448. Organic acids, behavior in the body, 389. Organized proteids or tissue-proteids, 457, 458. Orthonitrophenyl-propiolic acid, see Nitrophenyl-propiolic acid. Ossein, 37, 238, 240. Osteomalacia, 241 ; lactic acid in urine, 374. Otholiths, 284. Ovalbumin, 14 ; properties, 295 ; be- havior in the body, 284. Ovarial cysts, 288-291. Ovovitellin, 14 ; properties, 292. Ovum, 287. Oxalic acid, in blood, 114 ; in urine, 357 ; behavior in the body, 389. Oxalic-acid diathesis, 357. Oxalate lime in urine, 357 ; calculi, 432 ; sediments, 428. Oxalate stone, 432. Oxaluric acid, 251, 357. Oxaluria, 357. Oxamid, 16. Oxidation, 1-5, 71, 94, 95, 96, 155, 216, 217, 350, 363, 389, 391, 392. Oxyacids, formation of, in putrefac- tion, 216; passage of, into the urine, 216, 368; in sweat, 327. Oxy ben zoic acid, grouping in the body, 392. Oxybenzol, 391. Oxybutyric acid, in blood, 104; in urine, 384, 423. Oxygen, activity of, in the animal body, 5, 71, 95; in blood, 95, 96, 100, 101, 102; in intestines, 158, 170; lymph, 118; in stomach, 189; in transuda- tions, 122; binding of the oxygen in the blood, 70, 95; tension of, in the blood, 100, 101; in the expired air,. 101. Oxygen, consumed in activity and rest,. 265, 468; in starvation, 446; by the skin, 328. Oxyhaematin, 76. Oxyhaemocyanin, 83. Oxyhaemoglobin, 69; dissociation of, 70, 100; properties and behavior, 69- 71; quantity in blood, 69, 108-111; in muscle, 256; passage into the urine, 504 INDEX. 401; behavior with gastric juice, 183; to trypsin, 211. Oxynaphthalin, 391. Oxynitroalbumin, 17. Oxyphenylamido-propionic acid, see Ty rosin. Oxyproto-sulphonic acid, 17. Ozoue, 3, 96. Ozone-exciter, 96. Ozoue- transmitter, 71. Palmitic-acid, 245. Palmitic-acid cetyl-ether, 250. Palmitic-acid meiissyl -ether, 250. Palmitin, 243, 245. Pancreas, 199, 200; extirpation, action on the elimination of sugar, 407; charge, 132 ; change during secretion, 199, 211, 212. Pancreatic juice, 200; secretion, 200, 201, 211, 212 ; enzymes, 202-206; ac- tion on nutritive bodies, 202-206, 214, 215. Para-amido-phenol, 391. Parabamic acid, 351. Paracasein, 305. Paracresol, formation in putrefaction, 216, 363. Paraglobulin, see Serum-globulin. Parahaemoglobin, 71. Paralactic acid, relation to uric acid, 350, 351; properties and occurrence, 260 ; production in muscle during activity, 266, 268'; in rigor mortis, 263 ; in osteomalacia, 241 ; passage of, into the urine, 352, 374. Paralbumin, 289, 290. Paramyosiu, 253, 255. Paraoxyphenyl-propionic acid, 216, 369. Parapeptone, 182. Paraphenyl-acetic acid, 216, 368. Paraxanthin, 48, 359. Parietal cells, 175. Parotid, 167. Parotid saliva, 169, 170. Parovarial cysts, 291. Peutacumm, 325. Pentamethylendiamin, 376. Pepsin, 175, 178; properties, 178; in urine; 228, 376; in muscles, 256; de- tection in the contents of the stom- ach; 193; quantitative estimation, 180, 181; action on albumin, 179; on other bodies, 183. Pepsin digestion, 179, 180, 181, 183; products of the same, 26-29, 182, 183 186. Pepsin-glands, 175. Pepsin test, 180. Pepsin hydrochloric acid, 183. Pepsin ogen, 186. Peptone, 25; in the putrefaction of pro- teids, 216; in pepsin digestion, 27, 182; in trypsin digestion, 27, 206; as- similation, 231, 232; preparation, 29; nutritive value, 459; absorption, 227- 232; passage into the urine, 228, 398. Peptone-plasma, 54. Peptonuria, 398. Pericardial fluid, 122, 123. Perilymph, 284. Peritoneal fluid, 122, 124. Perspiratio insensibilis, 438. PETTENKOFER'S test for bile-acids, 146. Phacozymas, 283. Phaseomanuit, 258. Phenaceturic acid, 362, 393. Phenol, elimination by the urine, 216, 363; in starvation, 219; estimation in urine, 364; action on the urine, 394; electrolysis of, 6, 390; formation in putrefaction, 216, 363; behavior in the body, 216, 263, 393, 394. Phenol glycuronic acid, 364, 393. Phenol-sulphuric acid in urine, 363, 364, 393; in sweat, 327. Phenyl-acetic acid, formation in putre- . faction, 216; in body, 391, 392. Phenyl-amido-acetic acid, 392. Phenyl-amido-propionic acid, 391, 392. INDEX. 505 Phenyl-glucosazone, 410. Phenyl-hydrazine test, 307; in urine, 410, 413. Phenyl-lactosazone, 307. Phenyl-propionic acid, formation in putrefaction, 216, 361; in body, 392. Phlebin, 68. Phloridzin diabetes, 143, 407. Phosphorus-poisoning, action on the elimination of ammonia, 338, 384; of urea, 337, 338, 384; lactic acid, 374; production of fatty degeneration, 248; changing the urine, 337, 338, 374, 424. Phosphorized compounds in urine, 376. Phosphoric acid, elimination by the urine, 379-382, 448; formation in muscular activity, 266, 268. Phosphates in urine, 379-382, 395 (see various phosphates). Phosphate diabetes, 380. Phosphate stone, 432. Phenosin, 276. Phthalic acid, 391. Phymatorusin, 324; in urine, 403. Physetoleic acid, 250. Picric acid, reagent for albumin, 20, 400; for creatinin, 349; for sugar, 410, 414. Pig-milk, 312. Pike, flesh of, 272. Pilocarpin, action on the secretion of intestinal juice, 197; sweat, 327; saliva, 173. PIRIA'S tyrosin test, 208. Placenta, 298. Plants, chemical processes in the same, 1,2. Plasma, see Blood-plasma. Plasmoschise, 92. Pleural fluid, 128. Plums, influence on the elimination of hippuricacid, 360, 361. Polycythaemia, 111, 116. Polyperythrin, 325. Polyuria, 380, 387. Portal vein, blood from, 108, 141, 230. Potassium combinations, elimination in fevers, 383; in starvation, 383, 448; by the urine, 383, 448; by the saliva, 173; division in the form elements and fluids, 53. Potassium chlorate, poisoning with, 114, 401. Potassium phosphate, in yolk ef the egg, 294; muscles, 263; cells 53. Preputial secretion, 326. Principal cells, 175, 186. Propepsin, 186. Propyl-benzol, in body, 392. Prostatic calculi, 287. Prostatic secretion, 285. Protagon, 273, 274, 275. Protamiu, 287. Proteid, 31; of the mammary glands, 300, 319, 320; in the protoplasm, 42, 43. Proteids, separation from liquids, 21; approximate estimation in the, 400; influence on the formation of gly- cogen, 140; living and dead, 4; de- tection, 20; in urine, 394-398; quan- titative estimation, 21; in the urine, 399; absorption, 227, 229; passage into the urine, 394-399; heat of com- bustion, 471-473; digestibility of, in gastric juice, 182, 183, 190. Protein bodies, 13-40 (see various bodies). Protic acid, 256. Protocatechinic acid, in body, 365. Protoplasm, 42, 52, 53. Pseudocerebrin, 276, 277. Pseudomucin, 34; properties, 289. Pseudoxanthin, 258. Purple, 325. Purple cruorin, 72. Pus, 127-130; blue pus, 130; pus in urine, 404. Pus-cells, 127, 128. Pus-serum, 127. 506 INDEX. Putrefaction processes, 4 ; in intestines, 216-222, 363-369 ; regulation of the putrefaction in the intestines, 220, 221. Putrescin, 376, 425. Ptomaines, 11 ; in urine, 376. Ptyalin, 171 ; behavior with HC1, 172, 174, 212 ; action on starch, 171. Pyaemia, 113. Pyin, 14, 123, 128, 130. Pyiuic acid, 130. Pyloric glands, 175, 185. Pyloric secretion, 186. Pyocyanin, 130 ; in sweat, 328. Pyoxanthose, 130. Pyridin, in body, 393. Pyrocatechin, properties, 365; occur- rence in the urine, 365 ; in the supra- renal body, 134 ; in transudations, 125. Pyrocatechin -sulphuric acid, 363, 365. Pyromucic-acid, 389. Pyromucin-ornithuric acid, 389. Pyromucuric acid, 389. Quinic acid, behavior in the body, 360. Quinin, passage into the urine, 393 ; in sweat, 328 ; action on the elimination of uric acid, 352, 353 ; on the spleen, 132, 353. Rachitis, 240, 241. Reduction processes, 1, 2, 6, 7, 95, 96, 153, 155, 156, 218, 250, 360, 369, 370, 390. Reducing substances, production in putrefaction and fermentation, 4, 218 ; occurrence in blood, 4, 62, 218 ; in urine, 374 ; in transudations, 122. Rennet, 175-177 ; properties, 184 ; de- tection in the contents of the stom- ach, 193 ; passage into the urine, 376. Rennet-cells, 175. Rennet-glands, 175. Rennet-zymogen, 184. Resinous acids, passage into the urine, 393, 396. Respiration, of the hen's egg, 297 ; of plants, 2 (see Exchange of gases). Respiratory quotient, 447. Rest, exchange of material, 264-270, 467, 468. Retina, 280. Rhodophan, 282. Rhodopsin, 280. Rhubarb, action on the urine, 394. Rigor mortis of the muscles, 262, 272. ROBERT'S method for determining the quantity of sugar, 418. Rodents, biliary acids of, 147, 158. Rods of the retina, coloring matter, 280. ROVTDA'S hyaline substance, 67, 89, 129. Saccharogen, in the mammary glands, 320. Saccharic acid, 374. Sal ammonia, action on the exchange of material, 466. Salicylic acid, in the body, 392; action on the pepsin digestion, 181; on the exchange of material, 466; on trypsin digestion, 206. Saliva, 168-174; secretion, 174; poi- sonous properties, 11; mixed saliva, 170, physiological importance, 174; behavior in the stomach, 174, 187; various kinds, 168-170; action, 170; composition, 173. Salivary calculi, 174. Salivary diastase, see Ptyalin. Salivary glands, 165. Salmon, flesh, 271; sperma of, 287. Salts, see various salts. Salt-plasma, 55. Saltpetre, action on the exchange of material, 466. Samandarin, 326. Santonin, action on the urine, 394, 406. INDEX. 507 Saponification of fat, 203, 213, 244. Sarcolactic acid, see Paralactic acid. Sarcolerama, 251. Sarcosin, 257; behavior in the body, 389. Sarkin, see Hypoxanthin. SCHREINER'S base, 286. Sclerotica, 283. Scyllit, 181. Sebacic acid, 246. Sebum, 326. Sediments, see Urinary sediments. Sedimentum lateritium, 332, 354. Semen, 285. Semen-fibres, 285, 286. Semiglutin, 38. Serum, see Blood-serum. Serum-albumin, 14, 56 ; properties, 60; detection in urine, 397 ; quantitative estimation, 61, 399 ; behavior in the body, 60. Serum-casein, see Serum-globulin. Serum-fibrinogen, 62. Serum-globulin, 14, 56, 59; importance for the coagulation of the blood, 90 ; properties, 59 ; detection in the urine, 397 ; quantitative estimation, 60, 400. Senna, action on the urine, 394, 406. Sericin, 39. Sericoin, 39. Serin, 39. Serous fluids, 121-127. Shell-membrane of the egg, 35, 296. Sheep's milk, 312. Silicic acid, in feathers, 322; in urine, 385; in hen's egg, 294, 296. Sinkalin, 44. Skatol, 216; properties, 217; formation in putrefaction, 216, 363, 368; be- havior in the body, 217, 363, 368, 393. Skatol-carbonic acid, 368. Skatol coloring matters, 368. Skatoxyl, 216, 368. Skatoxyl-glycuronic acid, 368, 393. Skatoxyl -sulphuric acid, 363, 368; in sweat, 327. Skin, 322-329; secretion by the same, 326-329, 437, 440. Sleep, exchange of material, 468. Srnegma praeputii, 326. Soaps, in blood-semm, 62; chyle, 118; pus, 129; excrements, 222, 223; bile, 156; importance for the emulsifica- tion of fats, 214, 244. Sodium combinations, elimination by the urine, 383; division among form elements and juices, 53 (see various tissues and fluids). Sodium chloride, elimination by the urine, 65, 377; by the sweat, 328; im- portance physiologically, 451; influ- ence on the quantity of urine, 466; on the elimination of urea, 337; on the secretion of gastric juice, 176; quantitative estimation, 377-379; lack of, 451. Sodium phosphate in urine, 380; action on the exchange of material, 466. Sodium salicylate, action on the secre- tion of bile, 144. Sodium sulphate, absorption, 232; ac- tion on the metabolism, 466. Spectro-photometric method, 81, 82. Spermaceti, 250. Spermatin, 287. Spermatocele-fluid, 125. Spermatozoa, 286. Spermine crystals, 286. Spirographin, 34. Spleen, 130; relation to the formation of blood, 132; to the formation of uric acid, 132, 353; to digestion, 132; blood of, 109. Splitting processes, general, 1, 2, 7 (see various enzymes and ferments). Spongin, 14, 39. Staphylococcus, behavior in gastric juice, 192. Starch, hydrolytic splitting, by iutes- 508 INDEX. tinal juice, 197; pancreatic juice, 201; saliva, 171, 174; behavior in the stomach, 187. Starvation, action on the blood, 110, 115; on the secretion of bile, 143; secretion ofiudican, 219, 366; pancreatic juice, 199; on the elimination of phenol, 219; on the exchange of material, 443-449. Starvation cures, 480-482. Starvation diabetes, 407. Stearic acid, 244. Stearin, 243, 244. Stercobiliu, 153, 223. Stethal, 250. Stomach, importance of, in digestion, 191; self-digestion, 192; digestion in the stomach, 186-191. Stomach, catarrh, 193. Stomach, contents of, see Chyme. Stomach-fistula, 175. Stomach, glands of, 175. Stomach, mucous membrane of, 175. Stomach-saliva, see Pancreatic juice. Streptococcus, behavior in gastric juice, 192. Stroma of the blood -corpuscles, 66; of the muscles, 255 Stromafibrin, 67. Strychnin, passage into the urine, 393. Sublingual saliva, 169. Sublingual gland, 167. Submaxillary gland, 167. Submaxillary mucin, 32. Submaxillary saliva, 168. Succinic acid, in transudations, 121-126; in spleen, 131; in thyroid gland, 133; passage into the urine, 374; in sweat, 328. Sugar, formation, in the liver, 141, 142; after the extirpation of the pancreas, 407. Sugar, in stomach, 188; absorption, 229, 231, 232; relation to muscular activ- ity, 265-268 (see various varieties of sugar). Sugar test in the urine, 411. Sulphur, in albuminous bodies, 15; in urine, neutral or acid, 376. Sulphur-methaemoglobin, 75. Sulphocyanide of potassium, in saliva, 169, 170, 173; in urine, 376. Sulphuric acid, ethereal and sulphate sulphuric acid ; in urine, 363; estima- tion, 382; in sweat, 328; elimination by the urine, 363; relationship to the elimination of nitrogen, 382, 441. Sulphurous acid in the urine, 376, 389. Sulphuretted hydrogen, in intestinal putrefaction, 216, 218; in uriue, 376. Supra-renal body, 134. Sweat, 326; secretion, 327; action on the urine, 333, 335, 386, 387. Synovial fluid, 126. Syntheses, 1, 2, 7, 135, 140, 217, 248, 249, 336, 350, 360, 363, 366, 390, 392. Syntonin, 24; from muscles, 256. Sympathetic saliva, 168. Tartaric acid, 328. Tartar, 174. Tatalbumin, 294. Taurin, 151 ; behavior in the body, 389. Taurocarbaminic acid, 389. Taurocholic acid, 147, 148; quantity in various animal biles, 158; decompo- sition in the intestines, 219. Tea, action on the exchange of material, 467. Tears, 283. Teeth, 241. TEICHMANN'S crystals, 78, 79. Tension of the carbon dioxide in blood, 102-105; in the lymph, 118; of the oxygen in the blood, 100-102. Terpentine, action on the secretion of bile, 144 ; on the urine, 394 ; behav- ior in the body, 375. Terpenglycuronic acid, 375. Testicles, 285. Tetaniu. 11. INDEX. 509 Tetronerythrin,83, 325. Thallin, action on the urine, 394. Theobromin, 48. Theophyllin, 48. Thyreoprotein , 133. Tbyroidea, 133. Tbyroid gland, 133. Tbymus, 132. Tissue-fibrinogen, 88, 90. Tissue-proteids, 457, 458. Toluic acid, 392. Tol uric acid, 393. Toluol, behavior in the body, 360, 391. Toluylendiamin-poisoning, 162. Tonus, chemical, of the muscles, 264, 469. Tortoise-shell, 35, Toxine, 11. Transudations, 117, 121-127. Transudations in the intestine, 225. Tribrom-acetic acid, 17. Tribromamido-benzoic acid, 17. Trichlorbutyl-alcohol, behavior in the body, 390. Trichlorbutyl-glycuronic acid, 390. Trichlorethyl-glycuronic acid, 390. Trinitroalbumin, 17. Triolein, 245. Tripalmitin, 245. Triple phosphate, in urinary sediments, 430; in urinary calculi, 430, 432. Tristearin, 245. TROMMER'S sugar test, 409, 411 ; behav- ior to glycuronic acid, 375; uric acid, 354; creatinin, 348. Tuberculosis virus, behavior with gas- tric juice, 192. Tubo-ovarial cysts, 291. Tunicin, 323. Turacoverdin, 325. Turacin, 325. Trypsin, 201, 204; action on proteids, 205; on other bodies, 38, 210, 211. Trypsiu digestion, influence of various conditions on the same, 205, 206; products, 206. Trypsin-zymogen, 200, 211, 212. Typhotoxin, 11. Tyrosin, properties and occurrence, 208; in urine, 424; in sediments, 424, 430; detection, 209, 424; origin, 16, 35, 206, 216; behavior in putrefac- tion, 360, 363; in the body, 391, 392. Tyrosin-sulphuric acid, 232. Uraemia, blood, 115; bile, 158; contents of the stomach, 193; sweat, 328. Uramido-acids, 328. Urates, 354; in sediments, 332, 428. Urea, 336; elimination in activity and in rest, 267, 268, 467, 468; in starva- tion, 444; in children, 470; in disease, 337, 338, 383; after various foods, 337, 454-467; secretion of urea after meals, 458; properties and reactions, 339, 340; formation in the organism, 338, 339; quantitative estimation, 341- 347; splitting by ferments, 340, 427; synthesis, 336, 338; occurrence, 337. Ureids, 16. Ureometer, of ESBACH, 346 ; of DOKE- MUS, 346. Uric acid, 350; relation to urea, 350, 351; properties and reactions, 353, 354: formation in the body, 352, 353; relation of the spleen to the same 132, 352; quantitative estimation, 355, 356; synthesis, 350; behavior in the body, 351; occurrence, 351; in sweat, 328; in sediments, 332, 427, 428. Uric-acid calculi, 431. Uric-acid sediments, 332, 426-430. Urine, 330-433; secretion, 385, 386, constituents, inorganic, 377-385; poisonous, 11, 376; organic, patho- logical, 394-426; physiological, 336- 377; casual, 388-396; color, 332, 369, 388, 394, 405, 406; solids, calcula- 510 INDEX. tions of the same, 387; fermentation, alkaline, 374, 427; acid fermentation, 427; gases, 385; quantity, 386, 387, 388; physical properties, 331-336; reaction, 332, 427; degree of acidity, 332, 333; determination of acidity, 334; specific gravity, 335-387; deter- mination of, 336; passage of foreign bodies, 388-394; composition, 388. Urinary calculi, 430-433. Urinary coloring matters, 369-374, 405, 406. Urinary indican, 466, 467. Urinary indigo, 466, 467. Urinary sand, 430. Uriuometer, 336. Urobilin, 370, 371; relation to bilirubin, 153, 161, 370; to choletelin, 371; to haematin, 161, 162, 371; to haeinato- porphyrin, 371; to hydrobilirubin, 371; properties, 370-373. Urobilin icterus. 371. Urobilinogen, 369, 370. Urobilinoidin, 371. Urocanic acid, 377. Urochloralic acid, 390. Urochrom, 373. Urocyanin, 370. Uroerythrin, 373, 403. Urofuscohsematin, 403. Uro«;laucin, 370. Urohaematin, 370, 404. Urohodin, 370. Uroleucic acid, 365. Uromelanine, 370. Urophaein, 370. Urorosein, 370, 403. Urorubin, 370. Urorubrohaematin, 403. Urostealith stone, 433. Uroxanthin, 366. Uterine milk, 298. Vsilerianic acid. 16, 243. Vegetable acids,salts of, in the urine, 334. Vegetarians, nutrition, 476; excrement, 222. Vernix caseosa, 326. Visual red, 280. Visual purple, 280. Vitellin, 14; in the egg-yolk, 292; in the protoplasm, 42. Vitellolutein, 294. Vitellorubin, 294. Vitelloses, 27. Vitreous humor, 234, 282. Water, drinking, action on the elimina- tion of chlorides, 377; of urea, 337, 465; on the accumulation of fat, 465; on the secretion of bile, 144; on the secretion of urine, 385-387. Water, elimination by the urine, 385-387, 438; by the skin, 327, 438; in starvation, 447; importance for the body, 449; amount of, in the organs, 449; lack of water in the nutrition, 449. Whey, 312. Whey albumin, 305. White of the hen's egg, 294. Witch's milk, 316. Woman's milk, see Human milk. Wool-fat, 326. Work, influence of, on the elimination of chlorine, 377; on the exchange of material, 267, 269, 467, 468. Xanthin, properties and occurrence, 49; in urine, 359; in urinary sedi- ments, 430; quantity in the liver, 137; in pancreas, 200; detection and quan- titative estimation, 51, 52, 359. Xanthin bodies, general on the impor- tance and occurrence, 47, 48; in blood in leucaemia, 48; in urine, 359; rela- tion to muscular activity, 261 to the formation of uric acid, 353. Xanthin -stones, 433. Xanthocreatinin, 258, 350. INDEX. 511 Xanthophau, 282. Xanthoproteic acid, 17, 20. Xanthoproteic-acid reaction, 20. Xylol, behavior in the body, 392. Yolk, of hen's egg, 291. Yolk, membrane, 291. Zinc, IH the liver, 137; passage into the milk, 320. ZoOerythrin, 325. Zoofulvin, 325. ZoSrubin, 325. Zymogens, see various enzymes: Ren- net, Pepsin, and Pancreatic enzyme. llIRSITY SPECTBUM PLATE. 1. Absorption spectrum of a solution of oxylwmoglobin. 2. Absorption spectrum of a solution of haemoglobin, obtained by the action of an ammoniacal ferro-tartrate solution on an oxy haemoglobin solution. 3. Absorption spectrum of a faintly-alkaline solution of methc&moglobin. 4. Absorption spectrum of a solution of Ttcematin in ether containing oxalic acid. 5. Absorption spectrum of an alkaline solution of Jmmatin. 6. Absorption spectrum of an alkaline solution of hcemochromogen, obtained by the action of an ammoniacal ferro-tartrate solution on an alkaline- hsematin solution. 7. Absorption spectrum of an acid solution of urobilin. 8. Absorption spectrum of an alkaline solution of urobilin after the addition of a zinc-chloride solution. 9. 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