ge sren eee as i = ee ee epee ape : i SSS = aN = H if i sat a) ces wit oer mils DARL at A Nano Wee eh Ba Ai iain an My ele PART th iN , Hie bis * i gn Voi ue ANN fi Pony a eR y ays DAN) f We f i RAO a co HANI ‘ f By a ee i f | _ ea me ita hh ON hens Sent Ra Rey ERAN nod i Hs) ah Toa Mine ; TA WAG RUN ann 3} ’ Wien Meats Snr uy Uno aie 7 ¥ a 5 MNES Pee AN , ane AGH i , uy 1 oaneatirh Mi ait Mare nae ean Tuy ANE i ebro it, aaa AITO MWe enc i nuh Ve i hay d its : ates (ene ANY: uf i SR CO elaTito i A We rigs Miah it is AO A ANASTR i Rit my ae OTANI Aine ant i ite cy) Wy i A , ap i : mary Re NN pe sy i ‘ ray na Mn man Pech be hate y + Mt ty Ly Nye Fee it Ni rena tna Hein Na oh Sect can Hi oh : re . ae j Pati wl a f i) ay Uieay » petals nea SANT GAIL ath IN ait Pou sraitapaan ta BS at Vine mature Set Sar Sere Nena us Sten 4 ¥ TEE els OO tae ) FUTON Ete im Rae F : yy Onre ; Aes ans ih vr Ah i atl a Lay ks * ; Ce - Cay Ann rt ‘ Transactions OF THE Kentucky Academy of Science VOLUME ONE (1914—1923) DR. JOSEPH H. KASTLE oe TRANSACTIONS OF THE KENTUCKY ACADEMY OF SCIENCE VOLUME ONE (1914-1923) WILLARD ROUSE JILLSON, Sc. D. PRESIDENT OF THE ACADEMY AND EDITOR OF THIS VOLUME LEXINGTON, KY. 1924 THE STATE JOURNAL COMPANY Printer to the Commonwealth Frankfort, Ky. CONTENTS Page IPURCHE BIOS ca ee ae ae er es ne eae cer RE ari ean pe 6 OftiCersies (COMA N92 3) ore cee ere etic igre et aaecte seater th oud sewer el ieee sles c anne 9 Constitution cand “by-laws =<. ss: caisson we we ee ne ee soe oe bas 10 INO CT, OVO Oye ee oot cease iets oe ese stn Savane aliap erect lat Suess: Sonus Gis O sere stouar ele 14 Memberships list ClOW4 O23) ies icc chase dusts soe tesla oe waite lscuslevse anes 15 Call for the onganization meeting .. 2.6... ee eee eens ns 21 Minutes of the organization meeting ............--... eee eee eee 23 Seconidsjannual mee tin ose. es eceecc tei tee teeson ste) epansons toe eee seo le thom deranm ual ame 6 bin Pairs tes sc seccxs ie ace sso sic sols See one se ceenelery, cote a ecole estes 34 MH OULEMes AMM UA MC Chim Of cece lasz ccs snsisjers aeoers av sctuioe oa) so cetelotea Soatete oe 42 AH piste ts Fayaeer aed eb ery eC CELI say Serer pce eee es ose eee etree shia seve cceelore ope nnrenne 57 Sixtheeamnu alscmee him Os Pea ele 2s eis vy steast segs a soutien ci Qie ie emi casda situs saree 35 SEVEMt Mera mI een COLUM Oe ascars, chs etckoy uceaees io (ote ss a icts akceetereeas sis ve scolar: 2 JDiAAN DN GhnoRN WN A hen on oo noon 6 ooo Hoe Hon eommmnnaG 103 Ninéheeanmual = meetin Ose re sieeve Acocn. sie Soca erasnneains weenees eet 117 Membheramm i al ae CLV Or saree ciiece cre velovovsoce: steusro shell tyes eyes enegicosee 6 a 133 Obitwany-70 1 SAve TS Parle ria iecs ele telcos ci epeloseicnage lous esas steeowccecaeds iauegere™etsace 137 Resolutions on the evolution controversy..........0.cc cece cece aes 147 Resolutions of the A. A. A. S. on the evolution controversy........ 149 PREFACE During the last few years the growing need of a unit volume containing all of the transactions of the Kentucky Academy of Science has often been expressed. It has been repeatedly pointed out that such a volume would afford at once a ready source of reference to the scientific papers which have been presented at each of the annual meetings, and at the same time provide an accurate and available chronology of the activities and officers of the organization. A continued lack of funds in the treasury of the Academy, however, made the proposal, desirable as it was, quite impossible. A year ago, at the tenth regular meeting, the writer of these paragraphs realizing that the transactions of the Acad- emy would undoubtedly be withheld from publeation for many more years unless individual initiative provided the ways and means to a considerable extent, offered to underwrite the cost and edit Volume One of the transactions for the Academy. This proposal was accepted by the Academy. As a result, this book now appears in a limited edition sufficient, however, for our entire membership, the regular exchanges of the Academy, and the principal scientific libraries of the country and abroad. All papers herein presented, unless otherwise previously arranged, appear in abstract form as printed in ‘‘Science’’ In the preparation of this manuscript and the reading of the proof I have received most scholarly assistance and advice from Dr. A. M. Peter, the veteran secretary of the Academy. I have also been greatly aided by Dr. Peter’s personal secre- tary, Miss Ethel V. T. Caswell. Without this generous co- operation these transactions would necessarily have lacked much in the way of completeness and real value. Willard Rouse Jillson Frankfort, Ky., March 1, 1924. TRANSACTIONS OF THE KENTUCKY ACADEMY OF SCIENCE OFFICERS. £ Kentucky Academy of Science OFFICERS (1914-1923) Organization meeting, May 8, 1914, Dr. P. P. Boyd, Chairman. Charles J. Robinson, Secretary. 1914-1915. President, Joseph H. Kastle, Experiment Station, Lexington. Vice-President, N. F. Smith, Central University, Danville. Secretary, Garnett Ryland, Georgetown College, Georgetown. Treasurer, W. M. Anderson, University of Louisville, Louisville. 1915-1916. President, N. F. Smith, Central University, Danville. Vice-President, A. M. Miller, Univ. of Ky., Lexington. Secretary, A. M. Peter, Experiment Station, Lexington, Treasurer, Garnett Ryland, Georgetown College, Georgetown. Member Committee on Publications, R. T. Hinton, Georgetown College, Georgetown. 1916-1917. President, A. M. Miller, Univ. of Ky., Lexington. Vice-President, Garnett Ryland, Georgetown College, Georgetown. Secretary, A. M. Peter, Experiment Station, Lexington. Treasurer, Paul P. Boyd, Univ. of Ky., Lexington. Member Committee on Publications, Frank L. Rainey, Centre College, Danville. 1917-1918. President, R. C. Ballard Thruston, Louisville. Vice-President, J. EK. Barton, State Forester, Frankfort. Secretary, A. M. Peter, Experiment Station, Lexington. Treasurer, Paul P. Boyd, Univ. of Ky., Lexington. 1918-1919. President, J. E. Barton, State Forester, Frankfort. Vice-President, Paul P. Boyd, Univ. of Ky., Lexington. Secretary, A. M. Peter, Experiment Station, Lexington. Treasurer, J. S. McHargue, Experiment Station, Lexington. Member Committee on Publications, J. J. Tigert, Univ. of Ky., Lexington. 1919-1920 President, Paul P. Boyd, Univ. of Kentucky, Lexington. Vice-President, Walter H: Coolidge, Centre College, Danville. Secretary, A. M. Peter, Experiment Station, Lexington. Treasurer, J. S. McHargue, Experiment Station, Lexington. 1920-1921. President, W. H. Coolidge, Centre College, Danville. Vice-President, Geo. D. Smith, State Normal School, Richmond. Secretary, A. M. Peter, Experiment Station, Lexington. Treasurer, J. S. McHargue, Experiment Station, Lexington. 10 KENTUCKY ACADEMY OF SCIENCE. Member Committee on Publications, Dr. Paul P. Boyd, Univ. of Ky., Lexington. 1921-1922. President, Geo. D. Smith, State Normal School, Richmond. Vice-President, Lucien Beckner, Winchester. Secretary, A. M. Peter, Experiment Station, Lexington. Treasurer, Charles A. Shull, Univ. of Ky. Lexington. 1922-1923. President, Lucien Beckner, Winchester. Vice-President, John A. Gunton, Transylvania College, Lexington. Secretary, A. M. Peter, Experiment Station, Lexington. — Treasurer, W. S. Anderson, Experiment Station, Lexington. Councilor to A. A. A. S., A. M. Peter, Lexington. 1923-1924, President, W. R. Jillson, State Geologist, Frankfort. Vice-President, Austin R. Middleton, Univ. of Louisville, Louisville. Secretary, A. M. Peter, Experiment Station, Lexington. Treasurer, W. S. Anderson, Experiment Station, Lexington. Councilor to A. A. A. S., A. M. Peter, Lexington. CONSTITUTION OF THE KENTUCKY ACADEMY OF SCIENCE (As adopted May 8, 1914, and subsequently amended.) ARTICLE I—NAME. This organization shall be known as The Kentucky Academy of Science. ARTICLE II—OBJECT. The object of this Academy shall be to encourage scientific research, to promote the diffusion of useful scientific knowledge and to unify the scientific interests of the State. ARTICLE IIJI—MEMBERSHIP. The membership of this Academy shall consist of Active Members, Corresponding Members, and Honorary Members. Active members shall be residents of the State of Kentucky who are interested in scientific work. They shall be of two classes, to-wit: National members, who are members of the American Association for the Advancement of Science as well as of the Kentucky Academy of Science, and Local Members, who are members of the Kentucky Academy but not of the Association. Each alctive member shall pay to the Secretary of the Academy an initiation fee of one dollar, at the time of election. National members shall pay to the Secretary of the CONSTITUTION AND BY-LAWS. i Academy an annual assessment of five dollars,* payable Otctober Ist, of each year, four dollars of which shall be transmitted by the Secretary of the Academy to the Permanent Secretary of the American Association for the Advancement of Science, and one dollar shall be turned over to the treasurer of the Academy. Local members shall pay an annual assessment of one dollar, payable October first of each year. Corresponding members shall be persons who are actively engaged in scientific work not resident in the State of Kentucky. They shall have same privileges and duties as Active Members but shall be free from all dues and shall not hold office. Honorary members shall be persons who have acquired special promi- mence in science not residents of the State-of Kentucky and shall not exceed twenty in number at any time. They shall be free from dues. For election to any class of membership the candidates must have been nominated in writing by two members, one of whom must know the applicant personally; receive a majority vote of the committee on membership and a three-fourths vote of the members of the Academy, present at any session or, in the interim between meetings of the Acad- emy, the unanimous vote of the members of the council, present or voting by letter. ARTICLE IV—OFFICERS. The officers of the Academy shall be chosen annually by ballot at the recommendation of a nominating com- mittee of three, appointed by the President, and shall consist of a president, vice-president, secretary, treasurer, and councilor of the American Association for the Advancement of Science, who shall per- form the duties usually pertaining to their respective offices. Only the secretary, treasurer, and councilor shall be eligible to re-election for consecutive terms. It shall be one of the duties of the Retiring President to deliver an address before the Academy at the annual meeting. The Secretary shall have charge of all books, collections, and records that may belong to the Academy. ARTICLE V—COUNCIL. The Council shall consist of the President, Vice-president, Secretary, Treasurer, and President of the preceding year. The Council shall direct the affairs of the Academy during the intervals between the regular meetings and shall fill all vacancies occurring during such intervals. ARTICLE VI—STANDING COMMITTEES. The Standing Com- mittees shall be as follows: *A recent action of the A. A. A. S. requires the payment to be made to the permanent Secretary in Washington, who returns one dollar of each five to the Kentucky Academy. 12 KENTUCKY ACADEMY OF SCIENCE. A Committee on Membership appointed annually by the President, consisting of three members. A Committee on Publications consisting of the President, Secretary, and a third member chosen annually by the Academy. A Committee on Legislation consisting of three members appointed annually by the President. ARTICLE VII—MEETINGS. The regular meetings of the Academy shall be held at such time and place as the Council may select. The Council may call a special session, and a special session shall be called at the written request of twenty members. ARTICLE VIII—PUBLICATIONS. The Academy shall publish its transactions and papers which the Committee on Publications deem suitable. All members shall receive the publications of the Academy gratis. ARTICLE TX—AMENDMENTS. This Constitution may be amended at any regular annual meeting by a three-fourths vote of all active mem- bers present, provided a notice of said amendment has been sent to each member ten days in advance of the meeting. BY LAWS. I—The following shall be the order of business: 1. Call to Order. 2. Reports of Officers. 3. Report of Council. 4. Report of Standing Committees. 5. Election of Members. 6. Report of Special Committees. 7. Appointment of Special Committees. 8. Unfinished Business. 9. New Business. 10. Election of Officers. 11. Program. : 12. Adjournment. II—No meeting of this Academy shall be held without thirty days’ notice having been given by the Secretary to all members. T1J—Twelve members shall constitute a quorum of the Academy for the transaction of business. Three of the Council shall constitute a quorum of the Council. IV—No bill against the Academy shall be paid without an order signed by the President and Secretary. V—Members who shall allow their dues to be unpaid for two CONSTITUTION AND BY-LAWS. 13 years, having been annually notified of their arrearage by the Treasurer, shall have their names stricken from the roll. ViI—tThe President shall annually appoint an auditing committee of three who shall examine and report in writing upon the account of the Treasurer. VII—The Secretary shall be free from all dues during his term of office. VIII—AlII papers intended to be presented on the program or abstract of same must be submitted to the Secretary previous to the meeting. IX—These by-laws may be amended or suspended by a two-thirds vote of the members present at any meeting. 14 KENTUCKY ACADEMY OF SCIENCE, IN MEMORIAM They have crossed the river and are resting in the shade of the trees: Arthur M. Breckler, = O19: Alfred Fairhurst, 1843-1921 Joseph Hoeing Kastle, 1864-1916. James Oscar LaBach, 1871-1922. Alexander T. Parker, 1832-1922. John D. White, , 1920. Miss Cora Williams, O228 Charles O, Zahner, , 1918. Percy Kendall Holmes, 1880-1924. Charles IT. Brookover, 1870-1922. MEMBERSHIP. 15 COMPLETE MEMBERSHIP LIST—1914-1923. (Correct to March 1, 1924) §—Corresponding member. {—Honorary member. t—Life member. *—Dropped. +—Deceased. c-0.—Care of The date denotes the year of election to membership. Name and Address. Branch of Science. Allen, Harry, ’20, Experiment Station, Lexington.......... Chemistry. Allen, W. R., ’23, University of Kentucky, Lexington........ Zoology. *Anderson, F. Paul, 714, Univ. of Ky., Lexington...... Mechanical Eng. Anderson, H. C., ’23, W. Ky. State Nor. Sclf., Bowling Green....Physics. Anderson, W. M., 714, University of Louisville, Louisville..... Physies. Anderson; W..S., 215, Univ; of Ky., Iuexingtom ¢)3 2... .00.%. Genetics. *Andres, P. M., 719, 1870 Elmwood St., Toledo, Ohio........ Engineering. Averitt, S. D., 714, Experiment Station, Lexington.......... Chemistry. Baer, Louis, ’23, Univ. of Louisville, Louisvilie............ Chemistry. Bales, C. E., ’23, Louisville Fire Brick Co., Louisville........ Chemistry. Bancroft, Geo. R., 719, Univ. of W. Va., Morgantown, W. Va., Chemistry. *Barnett,b.,.220, Univ. of Kentucky, lexington: .:......5.:.. Physics. *Barr, T. J., ’14, Univ. of Kentucky, Lexington....Mining Engineering. *Barton, J. H., ’15, c-o Guif Refining Co., Huntsville, Ala. ....Forestry. Beckunermbucien, 220; Wainchesteriycc uc secs ect cers stevens Gs Geology. *Bedford, M. H., ’15, University of Kentucky, Lexington..... Chemistry. Beebe, Morris W., ’23, Univ. of Ky., Lexington...Mining & Metallurgy. Belknap William University ob alomisvalle= cic lose ce cclae« ssrastiae Benjamin, Leonard P., 723, Huntington, W. Va., ...... Bacteriology. Best, Harry, 721, Univ. of Kentucky, Lexington.. Sn ee Sociology. *Bitner an. /., 716, Univ. of Kentucky, Lexington: 3... 750... : Physics. siBilumenthals seal Gis Butta dN Ye coerce acd oe cet yceatinrees Chemistry. Boggs, Jos. S., 723, State Dept. Roads & Highways, Frankfort..... Eng. *Bories, Miss Marie, 719, 4601 Grand Blvd., Louisville........ Chemistry. IBORTICS Wiad) 25, MOUS Vall Ory seals cones cesta sespencie ots ote Mining Engineering. Boyd, P. P., 714, Univ. of Kentucky, Lexington.......... Mathematics. Poreclder WAL Mier TW ouisvallew sD. OWO Ne cas coeieettneies sic Chemistry. +Brookover, Charles, °22, Univ. of. Louisville, Lowisville........ Medicine. Brown, l. A., 715, Experiment Station, Lexington.......... Chemistry. *Brownell, Harry G., ’15, Bethel College, Russellville.......... Physics. Brownie Mlevaib.. 722) sAshamd ere. cures ciue srs ecrcsects fects eee Geology. *Bryant, T. R., 714, Experiment Station, Lexington........ Agriculture. §Bucher, Walter, ’22, Univ. of Cincinnati, Cincinnati, Ohio ....Geology. Buckner, G. Davis, ’15, Experiment Station, Lexington...... Chemistry. Bullock, H. E., ’22, e-o Midland Mining Co., Lexington ....Geology. Burroughs WG... 222.— Berea Colleces Berea gets... ee cto nee Geology. WButts, Charles, ’22, U. 8S. Geol. Survey, Washington, D. C. ....Geology. 16 KENTUCKY ACADEMY OF SCIENCE. Caldwell, Morley A., 715, Univ. of Louisville, Louisville....Psychology. *Carrel, W. J., 714, Univ. of Kentucky, Lexington....Civil Engineering. Chalkley, Lyman, ’22, Univ. of Kentucky, Lexington............ Law. §Clark, Friend E., ’15, Univ. of W. Va., Morgantown, ‘W. Va., Chemistry. Conn John zh 20 S38 oliviaru NOt mryitessncees ie sri cramer Chemistry. Coolidge, W. H., 717, Centre College, Danville.............. Chemistry. Cooper, Thomas P., ’18, Director Experiment Station, Lexington, Agri. (Coulter, ‘Stanley; 214 Lafayette, Imdiana . 2. tin. ower clecinernae Botany. “ACOA DOL, Aloo datss Alay, IDPHINAIIIE eK s Sod omodooooome goc Biology & Chemistry. §Cox, Benjamin B., ’22, State Univ. of Iowa, Iowa City, lowa..Geology. Craig, W. J., 720, W. Ky. State Normal School, Bowling Green.... SOUQv aoe uRnNH nine SHaand OoeodouMahe meome aa Physics and Chemistry Crandall; Albert, 3: 715, Malton, Wasconsim: 7.5 .)<:<10 1) ternenene! Geology Crider Ar Mas Ad SO hrevieportts lucene lace aie cesta lee cece theme tenn Geology. Crooks, C. G., 715, Centre College, Danville.............. Mathematics. Crouse, C. 8., 721, Univ. of Kentucky, Lexington...Mining Engineering. Crump. Ma He 715. Bowling Gre emt sire «wie ete) sre) ovelete cnet Geology. S@urri|er Wi, 2722) Syracuse w Ni Voie sa cece cain cisco Sects cel entire Geology. Curtis, H. E., 714, Experiment Station, Lexington.......... Chemistry. Dasher, Geo. F., 716, Bethel College, Russellville.............. Science. *Davies, Hywell, ’14, (Washington, D. C. .......... Mining & Statistics. Davis, J. Morton, 714, University of Kentucky, Lexington, Mathematics. qDay, Arthur L., *17, Director Geophysical Laboratory, Washington, DUE OL epee sre Een rine Si nari ey ine Tee eMC eRe Gib oro 6.0.0 Geology. qDetlefsen, J. A., 718, The Wistar Inst. of Anatomy & Biology, Phila delphi Pale wae en crate. crceyaebnene gos ane Sans see want eae gems Genetics. Didlake, Miss Mary L., 714, Experiment Station, Lexington, Ent. & Bot. Dilly, O. C., 719, Lou. College of Pharmacy, Louisville, Pharmacology. Dimock, W. W., ’20, Experiment Station, Lexington, Veterinary Science. Downing, H. H., 714, Univ. of Kentucky, Lexington...... Mathematics. Dutiield *sWall” Ward.22)-arlamesss sca acter accra ere Engineering. ABaston, He), 714. Springfield). Dl... ts c se < 6 wae Mining Engineering. Eichelberger, Marietta, ’20, Univ. of Ky., Lexington..Home Economics. Ervin, Walter E., ’21, Centre College, Danville............ Education. Eyl oWalliam< C5, = ./225< Iuexiniotoms cs acucnscmearcicier sions cera crete Geology. jRaihurst, Alfred. 7415. hexing tons Di 192M ce ser os ces Chemistry. Fergus, E. N., ’21, Experiment Station, Lexington.......... Agronomy. LAM eee DORE Ols Hea ay cee Gomme DoD Onan aoe ete ode obo oS Chemistry. *Fleming, W. R., 715, 519 Maple Ave., Newport, Ky. ......... Chemistry. Foerster, M. H., 716, Consolidation Coal Co., Jenkins........ Forestry. Siohs; i) Julins, 715. Pulse; (Okla momma esis 5 sien s wtere edapee cles Geology. Ford, N. C., ’23, W. Ky. State Normal School, Bowling Green, Agricul. Freeman, W. E., ’14, Univ. of Kentucky, Lexington..... Electrical Eng. Funkhouser, W. D., 719, Univ. of Kentucky, Lexington........ Zoology. Gabbert, W. R., ’21, County Agent, Lexington............ Agriculture. SGardnier:: Jo Ee obs Mull sar Oa acceso sr vous sack oust one a covonensreioeaionersronans Geology. MEMBERSHIP. aly Garman, H., ’14, Experiment Station, Lexington.............. Biology. Gentry, H: V., ’23, Lou. Gas & Elec. Co., Louisville........ Chemistry. *Calbenty Awe. 4. BurlineFoms ViermOmb.(.chs acs aavelemaieiecia ec cmec Botany. PGmil@aane, Vik digh. Zilisy Abmlop ery) INE INeS A aicivala dai ao done Jiao Physics and Eng. (Glenn, L. C., ’22, Vanderbilt University, Nashville, Tenn.....Geology. Good, E. 8., 14, Experiment Station, Lexington....Animal Husbandry. Gott, E. J., 718, Experiment Station, Lexington.......... Bacteriology. *Graham, Robert, *15, Univ. of Illinois, Urbana, Illinois........ Biology. Greenmeenebe 220) nam lek Ont is Myce cee atavsta/ evars aegevelivae accor rerne Geology. Grinstead, Wren James, ’21, E. Ky. State Normal School, Richmond, Ree dou sic a actee diye acute aNyrteterennclicetivanh ieclanalcgayiee toasSy we egret a toa apa Re Psychology. Gunton, John A., ’22, Univ. of Western Ontario, London, Canada, Chem. Harms, Miss Amanda, 719, Experiment Station, Lexington....Biology. VHart, E. B., 719, Univ. of Wisconsin, Madison, Wis. ........ Nutrition. §Havenhill, Mark, 719, Kansas State Ag’! College, Manhattan, Kans, Pepi Pa ei ehatlesiols ose sbe rere eran wea taneanetoual Sie aviardie ene uiamieee Farm Mechanics. Healy, Daniel J., 714, Experiment Station, Lexington...... Bacteriology. Hemmenway, Ansel F., ’21, Univ. of Arizona, Tucson...... Chemistry. §Hendrick, H. D., 714, Takoma Park, Washington, D. C. ...... Agronomy. Henry, Miss Ruby A., ’23, Louisville Girls’ High School...... Science. Hinton, Robert T., ’14, Georgetown College, Georgetown...... Biology. TOC MMOs mre nie eye alone MOK IMO LOM ce ccenussecsyeloreuet eran siuuersetcieveeareleieny aie Geology. xEotemeaneAtlen. 719) Miramonte ive cw sc sise © cele stelbve alloca am Chemistry. HEV LIn ama Ee ue Wiles Wie Accor avaycelfateustarace: si tery Stes aile) sene serene a\lenensisy No. 12).Sept: 17, 1917, pp. 633-637: ‘“The Geologie Strueture Which Explains The Accumulation Of Oil In The Irvine Feld:’’ A. M. Miller. The oil sand in the Irvine field is the Columbus magnesian limestone of middle Devonian age overlaid immediately by Ohio Black Shale of upper Devonian age. It seems probable that the oil was forced out of the shale into the porous lime- stone by differential capillary attraction of the oil and salt water, and working up the dip on the northwestern slope of the great Eastern Kentucky Syneline, became trapped on top of the saltwater against the plane of a fault which extends with some interruptions on the surface from near Irvine to near Campton. The mechanics of the process was illustrated by reference to a diagram drawn upon the blackboard, and maps were exhibited showing the positions of the producing wells in their relation to this Irvine-Campton, or Glencairn Fault. ‘*A Method Of Ashing Organie Materials For The Deter- mination Of Potassium:’’ P. L. Blumenthal, A. M. Peter, D. J. Healy and E. J. Gott. The authors have shown that potassium is lost mechani- eally and by volatilization of its salts during the ashing of organic material. While such losses cannot be entirely elimi- nated they may be materially reduced by conducting all such cperations in a muffle furnace and by preliminary treatment of the sample with nitric and sulfurie acids before ashine. The sample should be evaporated on a water-bath with about 5 ee of molar nitric acid and 5 or 6 ee of 1:1 sulfurie acid for each 0.7 gm. of organic material taken, and then ashed. The quantities of potassium determined ranged from 3 to 21 mgms and the maximum loss was 0.46 mgm upon the largest sample. See Jour. Ind. and Eng. Chem. Vol. 9, No. 8, p 7538, August, 1917. 48 KENTUCKY ACADEMY OF SCIENCE. ‘*Killing Frost, and Length of the Growing Season in Var- ious Sections of Kentucky:’’ By F. J. Walz, B. S., U. S. Weather Bureau, Louisville. In his operations the farmer every season is confronted with risks due to weather and climate. Among the risks that he faces each season are those of damage by late frost in spring, early frost in autumn, and a possible shortening of the growing season below that needed for the proper de- velopment and ripening of his crops. This paper summarizes and presents in the form of tables and charts the results of a study of the dates in Kentucky of the last killing frost in spring and first killing frost in fall, and the length of the growing season considered as the number of days be- tween these dates. The records of the regular stations of the U. S. Weather Bureau, and those of the co-operative sta- tions with 20 years or more of record, were carefully ex- amined and analyzed, employing mathematical methods used in the investigation of statistics and probabilities. The aver- age dates of last killing frost in spring and first killing frost in fall, the average number of days in the growing season, and the ‘‘standard deviations’’ from these averages were com- puted for each station, and consequent risks or probabilities determined. See Ky. Experiment Station Circular 19. ‘Some Factors Influencing Kentucky As An Oil State:’’ James H. Gardner. At the present writing (June, 1917) Kentucky stands in the limelight as a prospective oil state. Due to the fact that the Irvine District of Estill County has been extended over a large area together with the greatly renewed activity in the older Kentucky fields, operators are now turning their attention to the State as a whole. This is par- ticularly true of oil men from the Mid-Continent Field. So it appears that the latter part of this year and the early months of 1918 will forever settle the question as to the State’s poten- tial rank in the production of petroleum and natural gas. Test wells are to be drilled in nearly every county in the State and the most modern applications of petroleum geology are being freely used. Up to the present time most of the ‘‘wild FOURTH ANNUAL MEETING. 49 eat’’ work has progressed only to the mapping or leasing state but the high standing of the companies interested is a good indicator of the developments that are surely to follow. There are four important geological factors that are al- ways met in the search for new oil fields. When all of them are found to work in harmony great fields, like those of Okla- homa, Kansas and Texas or those of Pennsylvania, Ohio and West Virginia, are the result. Geological ‘‘structure’’, such as anticlines, domes ete., constitute only one of these factors. A large number of structures do not produce oil or gas. They may or may not produce salt water. Furthermore they may le in what would be considered favorable regions. In such eases the detail which may have been expended in mapping them is of no avail. Such conditions result from failure of one or more of the three other factors, namely either (1) there is no open ‘‘sand’’ or other porous medium under the struc- ture to serve as a retainer for oil and gas; or (2) there has never been present any salt water or other water in the sand to serve as a concentrating factor; that is, no gathering of oil and gas from a disseminated state to a commercial body; or (3) there is an absence of petroliferous shale or other fossil- bearing rocks that produce oil in a disseminated form. Now the future of Kentucky as an oil state depends on the four factors above mentioned: (1) structure; (2) sand; (3) water; (4) original oil. There can be no question about the State having three of the above points in its favor, namely (1) structure; (2) water; (3) original oil. There are numer- ous favorable structural conditions in various counties of the State. The rocks contain plenty of water and there are some good beds cf oil bearing shale. The Devonian Black Shale is particularly a splendid carrier of original oil. The fourth factor is, however, as yet to be proven of sufficient importance to place Kentucky in high rank as an oil state; namely, ‘‘sand’’, In great oil fields there are large bodies of sand or retaining reservoirs in close proximity to beds of oil bearing shale. There are frequently several such ‘‘sands’’ in the geo- logical column in close relationships to oil shale beds. In Kentucky the ‘‘sands’’ or ‘‘porous beds’’ near the Devo- mian Oil Shale are carrying most of the oil so far discovered. 50 KENTUCKY ACADEMY OF SCIENCE, In Wayne County these sands lie in the Waverly series above the Black Shale, but in other districts the oil is held below the shale in porous beds of limestone. This is true of the oil fields at Irvine, Cannel City, Campton, Menifee County, and other districts of Eastern Kentucky. In the coal basins of Eastern Kentucky and Western Kentucky there are a large number of beds of porous quartz sandstone: they he in the Chester and Pennsylvania series but in connection with these sandstone beds, oil shales must be proven to exist in order that any par- ticular structure may be found productive. If for instance a bed of oil shale like the Devonian Black Shale could be found sust above or below the Big Clifty Sandstone at the base of the Chester, then an anticline containing these beds at suf- ficient depth would most certainly make a big oil and gas field like those of Oklahoma; but it so happens that in a great many eases in Kentucky the oil shales do not lie near dependable porous reservoir rocks or else the porous sandstones in the bigher portion of the geological column, such as those above enumerated, do not have near them any great amount of typi- eal oil shale. In conclusion the writer desires to state it as his opinion that Kentucky is not to rank high as an oil state in comparison with many other areas in the United States where the four factors work in harmony and there are numerous porous sands near beds of oil shale; however, the writer wishes to empha- size the probability that a number of structures in Kentucky will find the four factors working together and will furnish new oil pools that will be highly valuable to these who are fortunate enough to discover them. Careful studies by geologists working in the State will serve to gather a great deal of important information in addi- tion to merely mapping suitable structural conditions in any particular locality. ‘““The Species of Nicotiana and Some Classifications of the Varieties of Nicotiana Tabacum:’’ G. C. Routt. The Index Kewensis lists 124 species of Nicotiana from many countries; 75 are native to South America and 24 to North America. Nicotiana tabacum and Nicotiana rustica are the FOURTH ANNUAL MEUTING. 51 only ones grown commercially for the production of the var- ious forms of tobacco and snuff. Plants of this genus were among the first used by the geneticists for experimental work in plant breeding, the first hybrids having been produced by Koelreuter in 1760 by applying pollen of N. paniculata to the stigmas of N. rustica. Much breeding and selection work has been done on N. tabacum. Prof. Comes of Naples gives 6 varieties of N. tabacum, viz: fruticosa, lancifolia, virginica, brasiliensis, havenensis and macrophylla. N. lanei- folia, to which the Kentucky Burley belongs, is a native of South America. N. havanensis, to which Havana cigar tobacco belongs, is a native of Mexico, having been carried to Cuba by the Spaniards. Dr. G. E. Anastasia, of Seafati, Italy, enum- erates only 4 varieties of N. tabacum, viz: havanensis, brasil- lensis, virginica and purpurea. The Academy then adjourned for lunch in the Phoenix Hotel grill room where about thirty members gathered at an informal luncheon in honor of Dr. Day. Upon the reconvening of the Academy at 2:30, Dr. Arthur L. Day delivered a very able and interesting illustrated lec- ture entitled: ‘‘The Voleano Kilauea in Action.”’ From a physical-chemical viewpoint the study of voleanic activity centers first on the nature of the participating ingred- ients, then on the condition of equilibrium or the progress of the reactions taking place between them. At the time of our visit all the three states of matter—gases, liquid, and solid —were found represented. Gases were emitted constantly in great volume and displayed nearly all the great variety of cloud forms which have been so frequently described in voleano lit- erature, except the violently explosive type, which has been rarely or never seen at Kilauea since the advent of the white man (1820). There was a liquid lava basin of oval shape some 600x300 ft. (Figure 1) ineclosed by a lava dyke or rampart built up from the surrounding floor of the basin by the tumul- tuous, spattering, splashing of the lava lake. Both floor and rampart are frequently overflowed when the lake is high and rising, and again great masses of it fall into the lake and are redissolved when it is low. The floor of the pit at the time of onr first descent had been completely overflowed but three VHONVIISN WO UALVUO ‘NISVE VAVI T ‘DI VONVIIS AO UALVU) ‘SASVO DINVOIOA DNILOATIOO °% ‘Out 54 KENTUCKY ACADEMY OF SCIENCE. days before and was reasonably level. The fresh lava had solidified to a dept of some 10 inches and was abundantly solid to walk upon but was still uncomfortably hot and the eracks were still glowing. Surrounding this floor are the walls of the pit, some 200 ft. high at the time of our first descent, and made up of the exposed edges of successive earlier overflows which individ- ually rarely exceeded two or three feet in thickness. The pit as a whole was about 1500 ft. in diameter, roughly cireular in plan and with nearly perpendicular walls except for the talus pile which extended about half way up the wall. All these diameters vary somewhat from day to day and consider- ably from vear to year with the state of activity in the basin. It will be of interest to record some of the observations made in the course of the extended study of this voleano, the purpose of which is to obtain definite information about the character of the chemical reactions which take place in an active voleano and in particular to determine the role played by the gaseous ingredients which are very important factors in both its chemical and physical activities. In many studies of voleanoes the gases have been allowed to escape entirely while in others they were not captured until the nature of the components was so much altered by oxidation or otherwise that their identification, to say nothing of the determination of their relative processes and the character of the equilibrium existing between them, has remained uncertain. On these broader ques- tions, which are laboratory problems, much work still remains to be done. It is, however, possible to offer evidence of the participation of water and of some of the other volatile in- eredients in the activity of Kilauea in advance of this detailed study which may require some. years before all the questions which have been raised are satisfactorily elucidated. The problem of collecting voleanie gases which are satis- factory from the chemical viewpoint is a most difficult matter. Hot gases of more or less complicated composition discharged from an active voleanie vent into the air undergo immediate and violent chemical and temperature changes, the conse- quence of which with our present limited knowledge of gas relations at these temperatures can be only partly inferred. FIFTH ANNUAL MHETING. Ol On It is therefore a matter of the first importance to eollect the gases directly from the liquid lava or the explosive vents be- fore contact with the air has given opportunity for altera- tions to oceur. We accordingly made the somewhat difficult descent into the erater without mishap and two erates, each containing ten glass tubes of one-half liter capacity each, were then lowered down to us. To one end of these series of tubes a glass pipe line was attached which led directly into one of the cracks in a lava dome (Figure 2) through which the gas was escaping. The gases discharged through this dome were free to escape only through narrow slits where they could be seen at night burning with a pale blue sheet of flame, thereby demonstrating (1) an excess pressure within and in conse- quence (2) that the gases released from the liquid lava came first in contact with the air on emerging from these eracks. The other end of the tube system was connected to a piston pump about four inches in diameter with a displacement of about 2 14 liters per stroke. The gases entered the pipe line at a temperature of about 1000 degrees. The pumping was kept up for fifteen minutes in order to make sure that the air originally contained in the pipe line and connecting tubes was displaced by the gases from the voleano. In this pipe line water began condensing with the first stroke of the pum» and at the end of fifteen minutes about 300 cubic em. had ac- cumulated in the collecting tubes. Insofar as this reconnaissance yields final results it shows that the gases evolved from the hot lava at the Halemaumau are nitrogen, water, carbon dioxide, carbon monoxide, sulphur dioxide, free hydrogen, free sulphur, together with chlorine, fluorine and ammonia in comparatively insignificant quantity. The first plain conclusion which follows from the discovery of this particular group of gases associated together at a tem- perature of 1000 degrees or more is that they cannot possibly be in equilibrium there and that chemical action between them is still going on. Whatever may have been the previous op- portunities for chemical readjustment among the gases as they arose in the magma and were gradually set free with the diminishing pressure, they are still in process of active re- action when discharged into the air. Free sulphur for example 56 KENTUCKY ACADEMY OF SCIENCE. eould not have remained in permanently stable association with carbon dioxide; neither could free hydrogen be found in stable association with CO2 and SO? at 1000 degrees. The consequence of the gradual release of these gases is the interreaction between the gases thus set free in constantly increasing quantity as the surface is approached. The reac- tions are accompanied by evolution of heat which obviously operates to raise the temperature of the surrounding lava so long as the reacting gases remain in contact with it. In full accord with the positive conclusion that these par- ticular gases cannot exist together in stable equilibrium at the temperature at which they are found, but are in process of active reaction, the record of the analyses shows their composi- tion to vary from one tube to another. It is therefore probable that the proportions of the individual gases change with every bubble which bursts from the liquid basin. Further confirmation of the same conclusion is found in the observation that when the gases given off by the lava in- crease in quantity, the quantity of lava remaining the same, its temperature increases, and conversely when less gas is dis- charged this temperature diminishes again. The measured change in the temperature of the surface lava during the per- iod of our observations amounted in maximum to 115 degrees. The heat generated by these gas reactions in the region near the surface when the amount of gas is large may well be more than sufficient to counteract the cooling effect of the expansion within the rising lava calumn which may thus be- come hotter and not cooler as it approaches the surface. It appears further from the order of magnitude of the quantities of heat thus released by chemical reaction within the voleano conduit that we have here happened upon an enormous store of voleanic energy which approaches its maximum tempera- ture at the surface itself. It is not certain at the moment that this discovery throws new light on conditions far below the surface, except perhaps to relieve us of the necessity of postu- lating extreme temperatures for the lava chambers below which on other grounds must be considered highly improbable. The Academy gave Dr. Day a rising vote of thanks. “I FIFTH ANNUAL MEETING. Ol BUSINESS SESSION The Membership Committee made a supplementary report presenting 3 more names for regular membership. The report was approved and these gentlemen were unanimously elected to membership. The Auditing Committee reported that they had examined the Treasurer’s report and found it correct. The report was approved and the committee discharged. The Nominating Committee reported the following nomina- tions for officers: Mormmbe resident css) c 2 woeeetireeh anes R. C. Ballard Thruston ory Vice Presidemt ss cces sities Wi ieee cy aceaterl aes hs J. E. Barten HOTS OCCRELATY ics iiarchc ciarke bee veh a Soecera Uussue rat days) allolenee A. M. Peter orm easumenic:. (uty stay e.te ef Seats le em cia ceral ons Paul P. Boyd The report was approved and these gentlemen were unani- mously elected. There being no other business, the Academy adjourned without date. (Signed) ALFRED M. PETER, Secretary. Ve MINUTES OF THE FIFTH ANNUAL MEETING Lexington, Ky., May 4th, 1918. The Academy was called to order in the Physics lecture room, University of Kentucky, by Vice-President Barton. The report of the Secretary was read and approved. REPORT OF THE SECRETARY OF THE KENTUCKY ACADEMY OF SCIENCE FOR THE YEAR ENDING MAY 4, 1918 As Membership Committee the President re-appointed Dr. G. D. Buckner (Chairman), Dr. P. L. Blumenthal and Mr. J. 8. McHargue. Of the seven persons elected to active membership at the last meeting, six paid their initiation fee and their names have been added to the list. KENTUCKY ACADEMY OF SCIENCE. ot ie 2) The status of our membership, as shown by the Secre- tary’s books May 8rd, is shown in the following table: Am’t due IAChVe Members; Ne SOOM StAN CIM Ogee cnued- cuctetetl leisy. ofetstetenetens 32 IMempberseiny aGrearse fOmiCUrrenGeyea Tee cmasmile istic etetentetons 33 $33.00 Memberss inv arrears or stWwO vVears) ..c. scr ai eerelcle era enols ll 22.00 Members-more than, 2) years, imearreans) bo KENTUCKY ACADEMY OF SCIENCE. The Effect of Manganese on the Growth of Wheat: J. 8. McHargue. Jour. Ind. and Eng. Chemistry Vol. 11, No. 4, p. Boo April, 1oa9. After reviewing briefly some noteworthy results ob- tained by previous investigators on the relation of man- ganese to agriculture, the author presented results ob- tained by growing wheat in manganese-free sand and in cultural solutions, with and without the addition of manga- nese. Wheat plants grown to within a few weeks of maturity in cultura! solutions containing manganese and others of the same age in which the manganese had been omitted, were on exhibition. Where manganese had been added to the cultural solutions the plants were apparently normal in every respect, whereas the plants grown in solutions containing no manga- nese showed a retarded growth in the blades, stalks and roots, as compared with the plants of the same age receiving man- geanese. There was evidence of lack of the proper development of chlorophyl in the plants receiving no manganese and the blades of these plants exhibited a drooping appearance in that they were not able to hold themselves erect, which was quite characteristic and not to be observed in any of the plants re- celving manganese. The author concludes from his experiments that manganese plays a more important role in the growth of wheat than has hitherto been suspected. Formation of Petroleum: ©. J. Norwood (By title.) Cryoscopic Work with an Ordinary Thermometer: C. C. Kip- linger. It has been found possible to read small temperature intervals on a common thermometer, within an accuracy of 1/100 degree, by measurements of the parallax on an auxiliary scale equipped with a sliding peep-sight. Several heretofore troublesome sources of error in the boiling point method of determining molecular weights have been eliminated by using but one point as reference on a ther- mometer scale, having established this point by the use of a SIXTH ANNUAL MEETING. 65 known substance with a high degree of purity. This pro- cedure eliminates the need of a calibrated thermometer. The use of the parallax method is suggested in the estima- tion of fractional parts of a scale division on other instru- ments than the thermometer. Generalization on the Mean-value Theorem: H. H. Down- ing, The speaker, applying the mean-value theorem which states for certain functions that f(x+h) —f(x) =h’f’(x+¢h), O<¢<1, to certain elementary functions, obtained interesting relations between @ and h. For the function f(x) = axs-+ bxe-+ cx + d the relation was an equation which was simplified by setting ¢ = ¢h. This equation in terms of ¢ and h as vari- ables represents a hyperbola with one focus, one vertex, the center, the other vertex, and the other focus, lying on the lnes whose equations are, respectively, ¢ =0,¢ =1/3h, ¢=2/3h,4 =h, g=4/3h. Magnolia fraseri: Does it Occur in Kentucky?: Frank T. McFarland. List of Fungi from Kentucky: Frank T. McFarland. An Equation Balance: E. L. Rees. A Method of Constructing the Graph of an Equation in which the Variables may be Separated: E. L. Rees. Protein Metabolism in the Growing Chick: G. D. Buckner and others. (By title.) See Bulletin 220, Ky. Experiment Sta- tion. Review and Observations on the Mosaic Disease of Tobacco: G. C. Routt: The author reviews the work of other investigators and reports observations of his own upon the disease in experimental plots of different. varieties of tobacco. He favors the view that the best way to combat the disease will be to develop a resistant strain of tobacco. 64 KENTUCKY ACADEMY OF SCIENCE. Dr. J. A. Detlefsen, of the department of genetics of the University of Illinois, addressed the academy on ‘‘Laws gov- erning the transmission of characters from parent to off- spring.’’ The speaker gave a brief review of the search by investi- gators for the cause or causes of evolution. He then. ex- plained the law for the transmission of mono-hybrids, di-hy- brids, and tri-hybrids. He presented these laws and illustrated them so well that there was left no doubt in the minds of workers in other fields that great progress has been made in genetics in recent vears. He threw upon the sereen the tables giving the result of his own breeding experiments to show how nearly actual counts agree with the mathematical expectation, in the laws of transmission. It is remarkable how nearly actual counts of animals bred agree with the expectation of what, by Men- del’s law, they should be. A vote of thanks was given Dr. Detlefsen for his valuable address. The Committee on Nominations reported the following nominations for officers for next year: Mor President: i464). eislu aes bok de as oda lows VOPR em onOm Bors Vace:President: ac... ssa. baa ee oe Paul P. Boyd Hor, secretary (sas er een cae A. M. Peter Mona Ureasunern ss anc cacn Ma oe ...-d. 9. Middareue For member of Publications Committee ...... J. J. Tigert Each of these was then duly elected. The Auditing Committee reported that the Treasurer’s ac- counts had been examined and found correct. There being no further business, the Academy adjourned sine die. The following members and visitors were reported at the morning session: J. EK. Barton, J. A. Detlefsen, A. M. Peter, F. K. Sutton, J. S. McHargue, G. Davis Buckner, Frank T. McFarland, 8. D. Averitt, W. D. Iler, W. S. Anderson, N. F. Smith, Linweod A. Brown, G. F. Reddish, Foster F. Elliott, SIXTH ANNUAL MERTING. 65 M. L. Pence, Jno. J. Tigert, E. L. Rees, H. H. Downing, N. M. States, C. C. Kiplinger, C. D. Killebrew, J. W. Pryor, Vernon G. Grove, W. E. Butt, S. A. Boles, R. F. Hemmenway, and the following from Berea: Maxwell Morgan, Dewey Trosper, C. B. Anderson, Cloyd N. McAllister, Alma B. Ackley. (Signed) ALFRED M. PETER, Secretary. VI. MINUTES OF THE SIXTH ANNUAL MEETING The sixth annual meeting of the Kentucky Academy of Science convened in the Physies Lecture Room of the Univer- sity of Kentucky at 10 o’clock A. M. on Saturday, May 3rd, 1919, President Barton presiding. Present, about 40 members and visitors. The Minutes of the last meeting were read and approved as well as the reports of the Secretary and Treasurer. Abstracts of papers presented at the last meeting were forwarded to ‘‘Science’’ for publication and appeared in that Journal under date of July 19, 1918, copies of which were duly sent to the members. Copy of motion by G. D. Buckner offering the aid of the Academy to the country during the war, was sent to Hon. R. C. Stoll, June 6, 1918, to be forwarded to the Government at Washington. In a circular letter sent out to the members November 12, 1918, an opinion was asked regarding the feasibility of chang- ing the date of meeting of the Academy to coincide with that of the Kentucky Educational Association. Your Secretary has received only two opinions to date—one in favor of the change and the other opposing it, namely, Mr. Ivan P. Tashof, of Washington, D. C. says: ‘‘T think it is an exeellent idea to hold the annual meeting of the Kentucky Academy of Science at the same time as the annual meeting of the Kentucky Eduea- tional Association. J think the Academy and various scientific men seattered over the State will be mutually benefited thereby. Undoubtedly the men interested in science and teaching in the High Sehools will be glad to join the Academy and no doubt they will be benefited by the splendid annual address which is delivered before 66 KENTUCKY AGADEMY OF SCIENCE. the members of the Academy each year. The idea is too good to drop and I hope to hear that the next meeting will be held in Louisville when the Kentucky Educational Association meets.’’ Prof. C. W. Mathews of the College of Agriculture, says: ‘‘Regarding location, of headquarters of the Kentucky Academy of Science, I feel that with a growing State University at Lexington there will probably continue to be a larger number of persons interested in membership located in and near Lexington than at Louisville or any other point, therefore, as I see it, would be opposed to changing to Louisville as headquarters’’. As no interest in the question was apparent and the mem- bers of the Council saw no need for the change, no further action was taken in the matter. In the same circular letter the members were informed that State Academies of Science are now counted as affiliated so- eieties of the A. A. A. S. and that they have the privilege of joing this Association without the payment of an initiation fee but only the annual dues of $3.00 which entitles them also to receive one of the publications of the Association. So far only two of our members have taken advantage of this offer, namely, Mr. Ivan P. Tashof, of Washington, D. C., and Mr. N. M. States of the University of Kentucky, both of whom were recommended by your Secretary for membership. Your Secretary desires again to call the attention of our members to the advantage of belonging to this Association and to urge that all who do not belong to the Association take advantage of this opportunity to join. Our membership is now 93. Of the 8 persons elected to membership last year all have paid their initiation fees and their names have been added to the list. Since our last meeting we have lost one member by death, namely, Dr. Charles O. Zahner, Prof. of Physiology in the Uni- versity of Louisville, who died of influenza, November 6, 1918. We have lost 3 members by resignation, namely, Prof. Geo. F. Dasher of Bethel College, Russellville: Prof. C. A. Nash, for- merly of Centre College, Danville; and Dr. Charles J. Robin- son, of the University of Louisville. One who has left the SIXTH ANNUAL MEETING. 67 State, Prof. C. C. Kiplinger, desires to be transferred from an active to a corresponding member. The total membership is classified as follows: RegulaGemembers. IN COO SUANGING: 5. <6 .fe ic cele esse! aaletal wl opsyane\o eos! 3, 3rer shece 30 heomaresmembers IM arrears efor) GUCS yas clatege cia: <)o.ssage. «(2 cave oo ape feos 45 Corresponding mem bersiiser saps. tel atenncs aloyorste le ci suerevalet seevsiin se stecsleustoqsnslrenos ets 12 OMOL A Wyye SIM CMI CL Sedyaycssesy.cs ar ska sess cosa ehclotesess east sonar a aes Gels ae ener desitts 6 93 The Secretary has sent two notices to each member in arrears and he desires to again eall their attention to the im- portance of attending to the matter at this meeting, Inasmuch as the by-laws provide that any who are in arrears for two years shall be dropped from the lst of members. The report of the Treasurer showed: iBalancesone hand sMayan4 sal OMS svors cesta ieee ccsecesye tone ia topo cceece ese oieester $11.51 IRECelVedetOTalGeS HANG (UES H oi sck ces sete occususrous, suates susie rome: shiny oc ove eseaieuieueee 41.00 HIRO Ga seem tei its tas h oats yn en cce aah vai ichs ont Seuviaccd cen suetia nal oaseeslow eer aaWease ee Ss 52.51 Paidstorsprocrams, Stavionery, CLC. 2... 02. 2.25.2 ees ee $27.17 andeomeclerlcal awOLkserimcs essences een cuser ee aera 10.00 BY foll7l HES LTT C ORO TNS TNL ING ea oe basi aces acs ee Rema nena oes $15.34 The report of the Membership Committee was read by Dr. Buckner, the Chairman, nominating 27 persons for active membership, 2 to be transferred from active to corresponding membership. All were unanimously elected. An open discussion was held as to the advisability of hold- ing our annual meeting jointly with the Kentucky Educational Association. The change was opposed by Dr. Terrell, who moved that the meetings be continued as heretofore. Motion seconded and passed by unanimous vote. Auditing Committee appointed by President Barton: A. M. Miller, Chairman; C. D. Killebrew and N. F. Smith. Nominating Committee appointed by President Barton: Dr. Henry Meier, Chairman; Dr. Terrell and Dr. Buckner. The following program was then rendered: President’s address, by J. E. Barton, ‘‘The Relation of Pri- vate Forestry to the Economic Interests of Kentuecky.’’ 68 KENTUCKY ACADEMY OF SCIENCE. It was brought out that there are no public forests in Kentucky, the large bodies of forest lands being privately owned, mainly by coal companies. The preservation of timber in Kentucky is therefore a problem in private forestry. It was considered desirable that the legislature should pass some law regulating private forests and stimulating timber develop- ment by suitable modification of the methods of taxing timber land. New Fossil Invertebrates from a New Fossil Horizon in the Coalmeasures of Eastern Kentucky: W. R. Jillson, State Geol- ogist. A new fossiliferous limestone horizon in the Coal Mea- sures of Eastern Kentucky has been discovered by the author who has done sufficient field work on it to demonstrate that it possesses features of fundamental stratigraphic importance to the unmapped geology of this section. A comprehensive collection of invertebrates taken by the author from an out- erop of this horizon on the Dr. G. T. Kendrick farm on the headwaters of Cow Creek, Floyd County, and identified by Professor Charles Schuchert, shows an incomplete list of about forty species of which ten are new and about sixteen very rare. It is a very unusual Pottsville fauna with the characteristic index forms absent. Three other widespread fossiliferous limestones in this same area are noted, all of which possess virgin stratigraphic potentialities. The author tentatively correlates them into the Norton (Middle) and Wise (Upper) Pottsville. A Phase of Evolution: W. S. Anderson. In every breed of animals it is found that a few are ex- ceptionally potent in passing on their good qualities. The author illustrated this from certain families of horses and advanced some speculations as to the possible cause. Electrolytic Solution Glow: Dean W. Martin. In December, 1917, the author observed a glow on the aluminum terminal of an electrolytic rectifier with lead and aluminum electrodes in a 10 per cent solution of sodium phosphate. It was found possible to produce the glow with SIXTH ANNUAL MEETING. ay) solutions of many different salts, of different concentrations, at temperatures from 0 to 100 degrees and with electrodes of aluminum, zine or magnesium and with voltages ranging from 80 to 1,500. A simple apparatus was exhibited and production of the glow was demonstrated. The observation is published for the purpose of learning whether others have noted or investigated the phenomenon. The Bacteriological Descriptive Group Number: D. J. Healy. The author has found it necessary to develop the group number of the Society of American Bacteriologists in such a manner that it will indicate the action of soil bacteria on nitro- venous compounds, organic acids and sulfur. The group num- ber, enlarged in this manner, has proved valuable in the study of soil bacteria. A Brief Discussion of Lexington Sewage Purification: H. D. Spears. A modern sewage-disposal plant operated by gravity takes care of 3,000,000 gallons containing 2 1-2 tons of sus- pended solids. The sewage passes through bar screens and grit chambers into Imhoff tanks, where bacterial action takes place and sludge is deposited. The effluent passes into ‘‘dosing tanks’’ which empty automatically every 15 minutes into fil- ter beds, 2 acres area, of coarsely broken limestone covered with broken granite, together 6 feet deep. Thence the efflu- ent passes through secondary sedimentation tanks and into a near-by stream. It is clear, odorless and has a ‘‘relative stability’’ of about 95 per cent. The sludge from the Imhoff tank is drawn off periodically into drying beds whence it is returned to the soil, when spadable. A Specimen of Lodestone from Kentucky: 48 Philosophical and educational sciences..................-. 15 EIGHTH ANNUAL MEETING. 105 Classified geographically and as to educational institutions our active membership is as follows: 53 from the University of Kentucky, Lexington, 5 from the University of Louisville, Louisville, 4°from Centre*College, Danville, 4 from Georgetown College, Georgetown, 2 from Berea College, Berea, 1 from Cardome, Georgetown, { 1 from the College of Pharmacy, Louisville, 1 from the Western Kentucky State Normal School, Bow- ling Green, 1 from the Eastern Kentucky State Normal School, Rich- mond, 1 from Cumberland College, Williamsburg. Not connected with educational institutions in the state are: 10 from Louisville, 4 from Lexington, 4 from Frankfort, 1 from Winchester, 1 from Bowling Green, 1 from Newport, 1 from Jenkins. Four active members are temporarily out of the state. Abstracts of papers presented at the last meeting were for- warded as usual to ‘‘Science’’ for publication and appeared in that Journal under date of July 9, 1920. Upon request of the National Research Council, the Secretary sent an account of the organization and activities of the Acad- emy and a list of members. President Coolidge appointed the following committees at a council meeting on June 14, 1920: © Membership: G. D. Buckner, Chairman; C. D. Lewis and J. S. MeHargue. Publications: P. P. Boyd was appointed in addition to Messrs. Coolidge and Peter who are members under the constitution. 106 KENTUCKY ACADEMY OF SCIENCE. Legislative: C. A. Shull, Chairman; R. C. Ballard Thruston and Lucien Beckner. Because of the absence from the state of Mr. J. S. McHargue, the Couneil of the Academy elected Dr. C. A. Shull treasurer, October 5, 1920. Under the terms of affiliation with the A. A. A. S. the Acad- emy is allowed a representative in the Council of the A. A. A. S. Such a representative should be elected at this meeting. The constitution of the Academy should be amended to pro- vide for this and any other changes required by the affiliation. The council of the Academy prepared such an amendment at a meeting on June 14th, 1920, but thru oversight the Secretary neglected to include it in the notice of this meeting, which pre- vents its being voted upon now, unless the resolution adopting the terms of affiliation may be construed as sufficient notice. Of our 21 corresponding members, only one has ever sent a paper or attended a meeting, as far as the Secretary is aware, the several are interested enough to acknowledge receipt of pro- orams and send good wishes. Your Secretary questions the ad- visability of carrying on our membership lst indefinitely the names of those who are not interested. Perhaps we should add a provision to the by-laws for dropping the names of those corresponding members who have not been heard from in a stated number of years. Respectfully submitted, (Signed) ALFRED M. PETER, Secretary. REPORT OF THE TREASURER FROM MAY 8, 1920, TO MAY 14, 1921 Charles A. Shull, Treasurer, in account with the Kentucky Acad- emy of Science. Receipts May 8, 1920—Balance on hand..............-.2+000- $ 21.84 Imitvatione fees and dies sac. s:. cai. sue cnere 226.06 $247.90 Disbursements Disbursements, including fees to the A. A. A. S. ...........- 153.00 ISMN) ChLIMNN ag eoebancunoaausoboboabodubOOd $ 94.90 The Membership Committee nominated 21 persons for active membership all of whom were duly elected. EIGHTH ANNUAL MERTING. 107 The report of the Legislative Committee was read by Dr. C. A. Shull as follows: Your Committee recommends the adoption of the following program for passage at the next meeting of the Legislature in 1922: 1—A law providing $1,000 for publication of the Proceedings of the Kentucky Academy of Science. 2—An inereased appropriation for the hastening of the com- pletion of topographic mapping of the State. 3—A request that a Soil Survey be begun, to follow as rap- idly as possible the topographic and geological mapping of the State. 4A law creating a Natural History Survey of the State, to center in the University, and to which all members of the Acad- emy could contribute. 5—Legislation to provide a Natural History Museum, for preservation of the collections secured by the Natural History Survey. The museum should be located at the University, where students could make use of the results of the Survey collections. 6—A law to increase the teaching of Science in the High Schools of the State, and compelling Boards to appropriate more for scientific equipment. 7—A law establishing a prize to be given annually for re- search work, open to all competitors in Kentucky in every line of scientific endeavor, the recipient to be determined by a com- mittee from the Academy of Science. 8—A law compelling each person or corporation drilling a well for oil or gas, or for other purpose to a depth greater than 100 feet, to file with the State Geological Survey a complete log of that well showing the formations gone thru, and a careful deseription of the location of the well so that it can be carefully mapped. 9-—Endorsement of the law now before congress to make of Mammoth Cave and its environs, a national park. 10—Ani act enabling the State of Kentucky to acquire and set aside for the benefit of future generations, such areas as are deemed worthy of preservation in natural condition, for pur- poses of study and enjoyment of nature. (Signed) CHAS. A. SHULL, Chairman. 108 KENTUCKY ACADEMY OF SCIENCE, The President then appointed the following committees: Auditing Committee: A. M. Miller, Geo. D. Smith and E. S. Good. Nominating Committee: P. P. Boyd, W. S. Webb, Henry Meier. Two amendments of the constitution covering the affiliation with the A. A. A. S., as ordered, at the Seventh meeting, were read by the Secretary and adopted as follews: Article III, MEMBERSHIP, paragraph 2, to read: ‘* Active members shall be residents of. the State of Ken- tucky who are interested in scientific work. They shall be of two classes, to-wit: National members, who are members of the American Association for the Advancement of Science as well as of the Kentucky Academy of Science, and Local Members, who are members of the Kentucky Academy but not of the As- sociation. Each active member shall pay to the Secretary of the Academy an initiation fee of one dollar, at the time of elec- tion. National members shall pay to the Secretary of the Acad- emy an annual assessment of five dollars, payable October 1st of each year, four dollars of which shall be transmitted by the Secretary of the Academy to the Permanent Secretary of the American Association for the Advancement of Science, and one dollar shall be turned over to the treasurer of the Academy. Local members shal! pay an annual assessment of one dollar, payable October first of each year.”’ Article IV, OFFICERS, to read: ‘‘The officers of the Academy shall be chosen annually by ballot, at the recommendation of a nominating committee of three, appointed by the President, and shall consist of a presi- dent, vice-president, secretary, treasurer, and councilor of the American Association for the Advancement of Science, who shall perform the duties usually pertaining to their respective offices. Only the secretary, treasurer and councilor shall be eligible to reelection for consecutive terms.’’ The following program was then rendered: President’s address: The Relation of Chemical Training to Industry: W. H. Coolidge. See Science, Vol. 54, No. 1399, p 367, Oct. 21, 1921. EIGHTH ANNUAL MEETING. 199 An Experiment in Mental and Physical Correlation: J. J. Tigert, University of Kentucky, Lexington, Ky. By title. Summary of the Thurstone Intelligence Tests for College Freshmen and High-School Seniors: Walter E. Ervin, Centre College. The average of 58 freshmen tested was 83, ranging from 30-39 (one student) to 150-159. The author remarks that such tests are not conclusive as to the mental equipment of any boy or girl, but they are helpful by placing the student in the school with more fairness. The Tragedy of the Passenger Pigeon: George D. Smith, Eastern Kentucky State Normal School. The author described his observation of the wholesale destruc- tion of the pigeons in their roosting place in a marsh, at night, by persons who came for miles around for this purpose, and hauled away the dead birds by the wagon load. This incident seems to have been one of the final stages in the extermination of the pigeon. The Last Warning of the Rattler: George D. Smith, Eastern Kentucky State Normal School. The paper describes a fight which the author observed be- tween a diamond rattlesnake and a large blue racer. The fight was long and fierce and ended in the destruction of the rattler. During’ the fight the racer is badly bitten by the rattler, hastens to a patch of weeds and bites several of the weeds, sucking out the juice. He then hastens back to renew the combat. In the progress of the fight the juice of the weed was applied a second time and the racer rushed back to renew the fight as before. Absorption in the Corn Grain: Charles A. Shull, University of Kentucky. Orthogenesis in the Membracidae: W. D. Funkhouser, Uni- versity of Kentucky. The attempt to expiain the remarkable developments of the pronotum in the family Membracidae by natural selection fails in the cases of the most bizarre and curious tropical forms. Poulton and others have suggested explanations based on pro- 110 KENTUCKY ACADEMY OF SCIENCE. tective coloration and mimicry which must be carried into the realm of speculation when applied to certain exotie species. Cer- tain genera, including Heteronotus, Centrotus, Pyrgonota and Spongophorus, seem to show very regular pronotal development along definite lines when traced from the more generalized to specialized forms. This is particularly true of the length and position of the suprahumeral, dorsal and posterior horns. These developments seem in many cases to be entirely without regard to utility and even to threaten the existence of the species. In comparison with the classical example of the Irish elk, many species of Membracidae seem to show even greater evidence of orthogenesis. The Progress of Kentucky in the Second Decade of the Twen- tieth Century: Edward Tuthill, University of Kentucky. Kentucky Petroleum Problems: Lucien Beckner. Kentucky offers many problems in petroleum geology which the consulting geologist and the geologist of the private company seldom have time to solve. The larger anticlines, the Cinecin- nati, north and south, and the Kentucky, east and west, present their peculiar characters that are not vet well understood. The author points out many problems which, could they be solved, would save the useless expenditure of thousands of dollars and probably result in the production of much wealth. The First Food of Young Black Bass: H. Garman, Experi- ment Station, Lexington, hy. A study of the food by use of the microscope on the stomach contents of both large and small-mouthed black bass, taken from the State Hatchery pools at Forks of Elkhorn, Kentucky, showed that the dietary of both species during the first five weeks of their active lives consists of small crustaceans belonging to the orders Cladocera and Entomostraeca, and of insect larvae be- longing to the dipterous family Chironomidae. The percentages of the different kinds of food were determined, and, as far as practicable, an exact determination was made of the crustacean species most prevalent in the dietaries. The purpose of the study was to learn just what food was most relished and how it might be influenced artificially for the benefit of young fishes produced at the hatchery. EIGHTH ANNUAL MEETING. 111 The Tolerance of Hogs for Arsenic: D. J. Healy and W. W. Dimock, Experiment Station, Lexington. There is a popular belief that hogs are not very susceptible to arsenical poisoning and an examination of the literature failed to disclose a record of arsenical poisoning in hogs. The results of four tests made by administering arsenic trioxid are given. The total of 11 shoats received large doses of arsenic trioxid; in some cases the doses were enormous. Nine of the shoats received, im addition to the arsenic, hog cholera virus. One animal died from acute arsenical poisoning, one from acute cholera, and one from an undetermined cause. It would appear from these results that young hogs possess a marked tolerance for arsenic trioxid. Growing Seedlings in Test-tubes with only Filter-paper Pulp and Distilled Water: Mary Didlake, Experiment Station, Lex- ington. The lower third of a test-tube is filled loosely with crumpled strips of filter paper, enough water to cover the paper is added and the tube plugged with cotton and sterilized in the autoclav. Sterilized seeds may be dropped in and allowed to germinate and grow. Soybean, cowpea, garden bean, garden pea, Canada field pea, vetch, alfalfa, red clover, Japan clover, velvet bean, peanut, locust, acacia, corn, wheat, hemp, and morning glory have been erown successfully in this way. Plants will grow thriftily for a month or six weeks. Effect of Frost and ‘‘Soil Stain’’ on the Keeping Quality of Sweet Potatoes: A. J. Olney, University of Kentucky. When the vines were cut away before frost, only 4 per cent of the potatoes spoiled after storage at about 60 to 65 degrees F. When the vines were cut immediately after a freeze, no loss occurred. When the vines were cut 5 days after the freeze the loss was 88 per cent. Potatoes badly affected with soil stain (Monilochaetes infuscans) but otherwise sound, sustained a loss of 55 per cent, while healthy checks suffered a loss of 12 per cent. Potatoes wrapped with paper sustained a loss of 20 per cent., as against 12 per cent in those unwrapped. Attempted Inter-species Crosses of the Genus Nicotiana: G. C. Routt. 112 KENTUCKY ACADEMY OF SCIENCE. Crosses were attempted among 7 species of Nicotiana. Of 911 flowers experimented with, 201 set seed. Only 4 of the 19 combinations proved fertile in both crosses and reciprocals, 4 proved fertile in one way only, and 11 proved infertile. Plants have not yet been grown from the seed obtained. The Production of Antitoxin: Morris Scherago, University of Kentucky. The method of producing diphtheria and tetanus antitoxin is described from the time the flasks of media are inoculated for the production of the homologous toxin until the antitoxin is ready for distribution. The factors influencing the potency of a toxin are discussed and the method of estimating the M. F. D. is outlined. The immunization of horses is discussed including the types of animals desired, preliminary treatment, dosage and time of injection. The time for taking trial bleedings and reg- ular bleedings is indicated and the standardization of antitoxin is briefly discussed. The method of concentrating antitoxin is also described and discussed. The Inefficiency of the Efficiency Expert: P. K. Holmes, M. D., Head of Department of Hygiene and Public Health, Univer- sity of Ky. Efficiency is the magie word today. It is caught up by every tongue. It is used most frequently and effectively in the business and industrial world. The head of the great business concern has to conduct the affairs of his great concern along ap- proved lines of efficiency. There must be centralization of con- trol; union to facilitate advantageous buying; stimulation toward increased production; methods for utilization of bi-pro- ducts; constant improvement in the quality and quantity of goods turned out; lessening of waste; elimination of useless and time consuming steps and muscular efforts; the application of scientifically worked out rest periods to increase production py decreasing fatigue; shortening of hours and increase of pay to make employes more contented; the introduction of safety de- vices; and many other things for the sake of efficiency. The whole keynote here is production—efficieney, the vision is focused only upon the product that is turned out, the ‘‘thing’’, 2? and in the final analysis the money which the ‘‘thing’’ repre- NINTH ANNUAL MEETING. 113 sents. In order to increase production mental and physical effi- ciency on the part of the employee must incidentally be in- creased. The conception of such a well organized plan of efficient production and the capacity for putting it into operation comes from the master mind only. It is almost unbelievable to think that the master mind that conceives of high standards of effi- ciency fails completely to apply the same principles to the body containing the brain which initiated the original idea; in other words, our men of big business fail at the crucial point and apply the principles to the creation and not to the creator. It is safe to say that if our busy business men applied these principles to themselves they would be able, thru better health, to plan even more efficiently for others. This plan of business efficiency will not permit of men who have to operate delicate and expensive machinery becoming in- eapacitated thru the effects of aleoholic poisoning. Quite a few heads of business concerns today are refusing to employ men or boys who use tobacco because indications point to a probable loss of working efficiency as a result of its use. It is probable that the person who largely depends upon coffee as an artificial stimulant to carry him thru his day’s work is placing himself on a lower level of efficiency. The man with weak flabby muscles and shortness of wind cannot long meet the demands of manual labor. The man who is being continually poisoned as a result of chronic constipation which results in loss of mental keenness and physical endurance cannot efficiently sell the products ere- ated by the concern which employs him. The man who, thru keep- ing late hours, over indulgence in aimless amusement, excessive stimulation of the emotions, overdraws upon his bank account of nervous force, is thus incapacitated for doing his best. These are some of the fundamental principles of business efficiency and repeated disregard of them by the employee means eventual loss of his position. But who is going to discharge the employer himself for falling below the standards of efficiency which he has set up for others? There is nobody to directly tell him that he will lose his ‘‘job’’ if he does not obey the ‘‘rules’’, but his business competitors who sometime will get this new con- 114 KENTUCKY ACADEMY OF SCIENCE. ‘ ception, will force him out of his ‘‘job’’ in the keen struggle for business supremacy. The averaga business man is probably not as clear a thinker and as enduring a worker by virtue of his natural endowments and observance of the laws of health as the average man he em- ploys. The average man of big business does not have time to live according to the laws of health. Theoretically he thinks this important but practically he shows that it is not. The result is that he shortens his working life here, but lengthens it in the possible life elsewhere. The average man of big affairs is one who has not paid much attention to the rules of health since he left college, if he hap- pens to be a college man. He may have gone thru four years of strenuous athletics there, but has done nothing of that nature since. His big powerful heart has gradually deteriorated into a smaller and rather flabby muscle—a power engine of much less working capacity—his lungs, because of lack of normal exercise, have diminished in breathing capacity and power, but have in- creased in susceptibility to disease. His muscles are smaller and weaker because they have had comparatively little use. What would happen if he had to run half a mile at a fair pace to meet an emergency? In the first place he could not run that distanee, but if he did attempt it he would be exhausted and perhaps ser- iously injured or possibly killed. There is no physiological rea- son why a man of forty or forty-five should not be able to safely and with positive benefit run a half mile or a mile at moderate speed. How many men can walk upstairs at a brisk pace to their office on the eighth floor without being out of breath for the next quarter of an hour He is very likely twenty or thirty pounds over weight, not of muscle or nerve or gland tissue, but of fat, and fat is an almost lifeless tissue—of no value in such excess—but a burden to carry as a pack on the shoulders of a civ- ilian going about his daily work would be. Further, it is an indi- cation of senility or premature old age and is so eonsidered by the life insurance companies. Again the organs of elimination have not been kept up to the highest point of efficiency and the waste accumulations and poisons are not properly gotten rid of and he is fatigued and tired when he should not be. He whe NINTH ANNUAL MEETING. 115 sits down all day in a luxuriously equipped office is often more tired at night than the employee who has been working hard on his feet all day in the machine shop or packing room. His appe- tite is poor; plain, coarse, wholesome food does not appeal to him. He must be tempted by delicacies or awakened by alco- holic stimulants. Any slight excess in eating causes a ‘‘hold-up’’ in the digestive machinery. There is no margin of safety there. He is more susceptible to disease because the body defenses are unable to cope with invading disease germs. Because of ner- vous tension, resulting from great responsibility, he has to re- store his nervous balance and comfort thru the use of artificial stimulants. He starts the day’s work on eaffein stimulation— gets up an appetite for dinner thru a cocktail—gets soothed from the irritations of the day’s work thru nicotine—gets rid of the body waste products thru the use of a cathartic—chases business cares away by amusement at the theatres and is finally lulled to sleep until the next day thru the kindly action of a sedative. To say the least, efficient living does not necessitate such a daily program. According to statistics, the death rate in America is higher for the middle period of life, which is around fifty, than in any other great nation in the world and this is more particularly true of men who carry great responsibility. Unfortunately this is the period of greatest usefulness. The diseases which are peculiar to men of this age in Amer- ica are called the ‘‘diseases of degeneration’’, some of which are heart disease, hardening of the arteries, apoplexy, Bright’s dis- ease or chronic inflammation of the kidneys, diabetes, ete. It is a matter of observation that an increasingly larger number of American men of prominence die as a result of one or more of these so-called ‘‘diseases of degeneration’’. What is the cause of these diseases? We do not as yet know. There is no one cause; it is probably due to a combination of causes and some of these which the indicator points to are: Lack of sufficient vigorous physical exercise, over-eating, use of stimulants such as coffee, tea, tobacco and alcohol; excessive social obligations, great -pro- fessional and business responsibility, ete. It is astounding to think that the efficiency expert is so in- 116 KENTUCKY ACADEMY OF SCIENOE. efficient when applying his principles to himself. This is one of those strange human inconsistencies. Most men are content to live on a low level of mental and physical efficiency. If we are able to get out of bed, take our three regular meals and do our day’s work, we say we are well, perfectly healthy. That is existence not health. Our standard of health should include bounding vitality and endurance, a spirit of optimism and in- vineibility resulting from efficiently working bodily organs. It should mean perfect digestion and elimination, capacity for rest- ful sleep, an irresistible desire for muscular expression in the form of enjoyable athletic recreation or its equivalent, a con- tented and well poised mind and a joy in just being alive. If a man is mentally efficient enough to create systems of efficiency in his business he should be efficient enough to apply them to his own life and follow them. Happily there is an increasingly larger number of men who are learning to live efficiently by taking time to play golf, attend eymnasium classes in the club or Y. M. C. A., work gardens, eat more rationally, and in general to obey the simple and ob- vious laws of health that any man with ordinary horse-sense would find time to obey. On the Trail of the Alaska Salmon: Dr. Henry B: Ward, Uni- versity of Illinois. The marvelous life history of the Alaska salmon has been worked out by the combined efforts of many investigators. In the early summer the adult fish appear off the coast, move for- ward into the inlets, start up stream, ultimately reach their spawning grounds, and having spawned, die. No adult salmon ever returns to salt water. The eggs rest in their gravel nests over winter and hatch out in the spring; the young fry play about in fresh water, descending slowly the streams until they disappear into the ocean. The markings on the scales carry a precise record of the age and wanderings of the fish in fresh water and in the ocean. Reasons for their move- ments in fresh water are not yet so well determined. The course they follow is very precise but the influences that direct it are still unknown. Partial explanations of the movements are to be found in the influences of the current of the stream and the NINTH ANNUAL MEETING. Af temperature of the water. The application of these principles to special instances indicates the extent to which they serve to explain the complex problems involved in migration. The author described many of his observations while studying the salmon in Alaskan waters. He also brought out forcibly the importance of Alaska’s natural resources, of which the salmon is one of the greatest. A short business session was then held. The President appointed a Membership Committee as fol- lows: Dr. G. D. Buckner, Chairman; Dr. H. Garman and Mr. J. S. MeHargue. The Auditing Committee reported the Treasurer’s accounts correct. Accepted. The Nominating Committee reported the following nomi- nations for officers: Hors President....0.. 3... Prof. George D. Smith, Richmond For Vice-President...... Mr, Lucien Beckner, Winchester MoreSecretary i.e. ss Dr. A. M. Peter, Lexington MOR eASUITER: 2c). sels «'s Dr. Charles A. Shull, Lexington. The report of the committee was adopted and the officers were duly elected. The new President then took the Chair, and made a short talk. The meeting then adjourned. (Signed) ALFRED M. PETER, Secretary. IX. MINUTES OF THE NINTH ANNUAL MEETING The ninth annual meeting of the Kentucky Academy of Science convened in the Physics lecture room of the University of Kentucky at 9:45 o’clock A. M., on Saturday, May 20, 1922, President George D. Smith presiding. Present: about 40 per- sons in the morning and 75 in the afternoon. The minutes of the last meeting were adopted without reading. The report of the Secretary was read and accepted. 118 KENTUCKY ACADEMY OF SCIENCE. SECRETARY’S REPORT Of the 21 persons nominated for membership at the last meeting, 15 paid the initiation fee and became active members of the Academy—9 becoming national members and 6 local members. We have lost one member by death since the last meeting, Dr. Alfred Fairhurst, formerly of Transylvania University. Four members have been dropped from the list on account of removal from the state and 9 for nonpayment of dues. The total membership is now 128, including 54 national members, 46 local members, making a total of 100 active mem- bers; 20 corresponding members and 8 honorary members. The membership may be classified as follows: Regular members in good standing.............. 79 Recular members) 1) Anvears.. «ce sci +. acer 21 Correspondiniewmembers wc yeu erie ese 20 On OTAT ys MEM) CLSisesee arto cl cerca che lrcneie aoe 8 128 In accordance with a resolution passed at the last meet- ing, copies of a pamphlet entitled ‘‘ Preservation of Natural Conditions,’’ published by the Ecological Society of America, have been purchased and distributed to the membership. President Smith, on December 29, 1921, appointed the fol- lowing persons to co-operate with Dr. Middleton in his work for the Ecological Society of America: Prof. C. D. Lewis, Berea, and Prof. F. L. Rainey, Danville. On account of Dr. Shull’s removal from the State, President Smith appointed Dr. Wren Grinstead of Richmond in his place on the Legislative Committee and Prof. W. S. Anderson in his place as Treasurer. Abstracts of papers presented at the last meeting were forwarded promptly to ‘‘Science’’ for publication and appeared in that journal under date of August 19, 1921. This being our only means of publication, it is important that members supply the Secretary with short abstracts of their papers for this pur- pose without delay. (Signed) ALFRED M. PETER, Secretary. NINTH ANNUAL MEETING. 119 The report of the Treasurer was passed as Prof. Anderson was not present. The report of the Council was called for and the Secretary explained the activities of the Council were concerned mainly with arrangements for the annual meeting so no formal report seemed necessary. The report of the Membership Committee was read by Dr. Buckner, Chairman. The report was adopted as read, after which the following were unanimously elected to membership : 15 active members, 3 corresponding members and 5 honorary members. The report of the Legislative Committee was ealled for but it appeared that no business had been transacted in this com- mittee. The report of the Committee to co-operate with Dr. Middle- ton of the American Society of Ecology was ealled for but in the absence of the Chairman of this committee none was pre- sented. President Smith then appointed the following committees: Auditing: Averitt, Vaughn and McHargue. Resolutions: Meier, Healy and Gunton. Nominations: McFarland, Valleau and W. 8S. Anderson. Under the order of new business, Dr. Terrell moved that all papers be short, thus allowing time for discussion. Dr. Buck- ner moved to amend so as to limit the time for discussion. The amendment was lost and the original motion was carried. The following program was then rendered : The Boleti of Kentucky: G. D. Smith, Eastern Kentucky State Normal School (President’s address). Colored lantern slides and stereoscopic photographs of 37 species of boleti observed in the vicinity of Richmond were pre- sented and explained. Factors Affecting the Germination of the Sclerctia of Clavi- eeps (Ergot of rye): Frank T. McFarland, University of Ken- tucky. 120 KENTUCKY ACADEMY OF SCIENCE. Most mycologists are fairly well acquainted with the method of germination of sclerotia of Claviceps, but there still remain several factors which are poorly understood. During the past two years, the writer has been engaged in a study of the scle- rotia of ergot from various countries. In the course of these investigations it has been found that sclerotia more than one year old failed to germinate. Sclerotia sown out of doors, on the surface of the soil, without any covering showed good germina- tion of the sclerotia with many well-formed stromata but the stalks usually are short. Some mycologists seem to have the idea that these sclerotia may have the power to retain their germination ability for more than one year. It is quite unlikely that any sclerotia under out-of-door conditions should remain dormant during the first spring after their maturity and germi- nate the second season. Sclerotia of Claviceps must go through a period of rest. The shortest period of rest so far found is about eight weeks. During this time when the sclerotia are at rest, they must be kept stratified in moist sand. Removal of the cuticle of sclerotia with a scalpel does not prevent the germina- tion, but the stromata are nearly always deformed, and all seem to rise from a stromatie cushion. Treating the sclerotia with a 5 per cent and a 30 per cent. NaCl salt solution, and then com- pletely removing all traces of the salt and stratifying the scle- rotia in the usual manner did not injure their germination power. The Role of Manganese in Plants: J. S. McHargue, Kentucky Agricultural Experiment Station. Jour. Amer. Chem. Soe. Vol. 44, No. 7, July, 1922, p. 1592. The purpose of this investigation was to determine if man- ganese has any definite function to perform in plant economy. The method of attack has been the preparation of plant nutrient compounds and quartz sand, free from manganese, and the erowing of plants in different portions of nutrient solutions or sand cultures from which manganese was withheld and in an- other equal number of portions of these media to which man- ganese was added. All the plants were grown until those that received manganese showed signs of fructification and a few to maturity. The plants from which manganese was withheld made a normal growth for about six weeks only. Thereafter they be- NINTH ANNUAL MEETING. 121 came chlorotic and the young leaves and buds died back and the plants made no further growth of any consequence, whereas the plants to which manganese was available grew in a normal way and fructified where the plants were grown to that state of maturity. The author concludes that manganese is necessary in the plant economy and that, therefore, eleven elements are neces- sary for the normal growth of autotrophic plants, whereas it has been taught previously that only ten are necessary. The Hydroxy-anthraquinone Derivatives in Plants: John Aberdeen Gunton, Transylvania College. A resume was given of the various plants containing deriva- tives of this type as well as a description of the forms in which these occur. The cathartic principles of cascara, senna, rhubarb, aloes and buckthorn were shown to be irritant anthracene deriva- tives that exist in the plant in the form of glucosides to which the physiological action is presumably due. Plants containing these bodies are found widely distributed throughout the globe and present an interesting stage in the chemical evolution of plant life. Considerable remains yet to be done on this group from the analytical and synthetical standpoints. Some Seed-borne Diseases of Agricultural Crops: W. D. Val- leau, University of Kentucky. Further studies on the extent of seed infection of corn with Fusarium monliforme confirm previous reports that it is prac- tically universal. The organism is carried between the various seed-coat layers and may extend in as far as the aleurone layer. In very flinty corn the organism remains dormant a longer per- iod after the seed is planted than in the poorly filled starchy kernels. A preliminary study of 8 lots of barley from 3 states, 12 lots of oats from 4 states, and 38 varieties of wheat from 5 states indicates that small grains are infected to a higher de- gree with pathogenic organisms than has generally been sus- pected. Morphological studies of lettuce seeds have demon- strated the presence of an organism in a high percentage of seeds which is believed to be the causal organism of lettuce root rot. The universal presence of root rot on clovers and the results of preliminary tests of seed infection suggest that 122 KENTUCKY ACADEMY OF SCIENCE. the causal organism is constantly present in clover seed. Ob- servations on crops affected by seed-borne root disease organ- isms, grown under different seasonal conditions, suggests that these organisms may play an important part in geographical and seasonal distribution of certain wild and crop plants. A Preliminary Report on a Study of Various Clovers as Found on Three Soil Experiment Fields of Kentucky with Spec- ial Reference to Root Systems: E. N. Fergus and W. D. Valleau, University of Kentucky. An ecological and pathological study is being made of various elovers, particularly red clover, growing on three soil types of Kentucky, in order to determine the causes of clover failure. Actual counts showed that red and alsike clover stands were practically equal throughout the first year whether on produc- tive or ‘‘clover sick’’ soils. Much diminution of stand oceurs on most soils during the second summer, reaching 100 per cent. on the least productive soil. Root rot was present to some extent on all root systems examined. Those developed in least pro- ductive soils were badly diseased or dead at the end of the first season. All tap root systems examined were badly diseased or dead at the end of the second season. The persistence of a clover plant after death of the tap root system depends on its ability to produce new roots from the crown. Extraction of Crude Oil by Means of Shafts and Tunnels: Henry Meier, Centre College. This ; method ‘of | recovery ~ of oil] dromeasbeds has been successfully carried on in Alsace since 1917. Experience has shown that by means of wells and pumps not more than 20 per cent. as a maximum of the oil contained in a bed can be brought to the surface. The recovery by sinking a shaft and digging tunnels through oil-bearing sand enables the recovery by seepage and by treating the sand with hot water, of two and a half times as much oil as by means of wells. This method of recovery increases the value of a concession. It opens to countries whose oil-bearing regions seem to have reached the end of production, new and encouraging prospects. Depletion of Kentucky Crude Oils: Willard Rouse Jillson, Director of the Kentucky Geological Survey and State Geologist. NINTH ANNUAL MERTING. 128 Since the year 1900, when, with a production of 62,259 bar- rels, the petroleum producing industry may rightly be said to have gained its feet in Kentucky, there have been many who have held it impossible for the State to become an oil producer of national importance. Others on the contrary, and to them we owe unmeasured gratitude, have steadfastly claimed a bright future for the oil operator in Kentucky. An analysis of the erude oil production figures for Kentucky for the 22 years which have elapsed since 1900 will do much toward presenting the truth of the matter, and aid in arriving at depletion esti- mates of future petroleum production for the Commonwealth. Although oil was first produced in Kentucky on the South Fork of the Cumberland River in 1819, nothing came of the dis- eovery for many years. The increasing number of ~‘wildeat’’ wells which were drilled in various parts of the State served to index oil producing areas rather than to augment the annual total of barrels produced. The earlest oil production figures of which we have an aecurate account are for 1883, and show only 4.755 barrels for the whole State of Kentucky. From this time on until 1899 the total annual production of the State ranged up and down from 3,000 to 9,000 barrels. At the end of the eighth decade following the first oil strike in Kentucky, it would have been impossible for anyone to have predicted with accu- racy what the future score of years, now passed, might have brought forth. In the same way it is hazardous at the present to predict the rise and fall of erude oil production in Ken- tueky, though with a certain fairly large production now es- tablished, and with the State pretty generally ‘‘wild-catted’’ by geologists and operators alike, there is considerably more to work upon now than there was 20 years ago. Certain established factors based on the economic and in- dustrial life of this country, such as the increasing demand for gas engine gasoline, industrial lubricants, and fuel oil, coupled with the central eastern location of this State will tend as the years go by to keep Kentucky well up in the ranks of the oil producing states. These factors have operated in New York and Pennsylvania for many years to put off the day of final and complete depletion. The same principles are now operating 124 KENTUCKY ACADEMY OF SUIENCE, in Kentucky, and will continue to operate even more vigorously in the future. A review of the production figures for Kentucky, when plot- ted in the form of a curve shows a well defined cycle of acceler- ated, followed by declining production extending from the year 1900 through to the year 1910. This period witnessed the devel- opment of Ragland, Wayne County, Campton, Irvine, Bussy- ville, and Fallsburg pools. From a minimum production of 2,259 barrels in 1900 oil production rose to a peak of 1,217,337 barrels in 1905, which was essentially maintained through the year 1906 when 1,213,548 barrels were produced. Crude oil production then dropped until 1910 when only 468,774 barrels of petroleum were produced in the State of Ken- tucky. This figure continued to be about the index of produc- tion through to the year 1915, when the impetus brought about by the wartime prices of gasoline, lubricants, and fuel oils, ex- pressed itself in a greatly renewed interest in ‘‘wild-catting’’, and resulted in a substantial increase immediately. Petroleum production jumped in 1916 to 1,144,750 barrels, and in 1917 and 1918 successfully passed the three and four million barrel mark. The peak of the recent extensive oil production in Kentucky was attained in 1919 when a total of 9,226,473 barrels, and a po- sition eighth among the oil producing states were secured. This advance, great for any Appalachian State, carried with it a vast amount of speculation by Kentuckians and others, which was largely responsible for the development. A slowing up of industry following the close of the war, shortly induced very much lowered prices for Kentucky erude oil and cast a doubt in the hearts of producers. In 1920 the total production of the State dropped to 8,546,027 barrels. The interest in oil development, however, had been sufficient during the five years preceding to bring about the discovery of several large producing pools widely distributed throughout the State of Kentucky. Chief among these in the order of their product- ivity were the Big Sinking, Ashley, Ross Creek and Station Camp; and other smaller pools in Estill, Lee, Powell, and Wolfe Counties; the grouped pools of Allen, Barren, and Warren Counties; the associated pools of Johnson, Magoffin and Lavw- rence Counties; and the widely extended pools of Wayne, Me NINTH ANNUAL MEETING. 12: On Creary, Clinton and Cumberland Counties. A strengthening of the price of crude oil resulted in the extension and stabilization of the oil producing industry in Kentucky during 1920. At the end of the year petroleum production amounted to 9,080,845 barrels, an increase of 534,818 barrels during the year. To the casual observer this increase has little of significance, but when one considers that the principal producing pool of the State the ‘‘Big Sinking’’, in Lee County, decreased steadily at a rate averaging nearly 10,000 barrels a month, it will be seen that this increase really meant the discovery and large develop- ment of other new pools elsewhere in the State. These pools were those of the Johnson, Magoffin, and Lawrence County ree- ion, and those of the Allen and Warren County region. A state- ment of the amount of petroleum produced in Kentucky from 1883 to the present time follows: PRODUCTION OF PETROLEUM IN BARRELS IN KENTUCKY FROM 1883 TO 1922* AGRE: eA eae ert eae Nie ieee iar eC RD ENG Mraricng rte 4,755 FUNG 2 ge aR ice ak NE ce 4,148 TS MM ee a Ce athe a oat mcliauneene RO ae Nay 5,164 TOO MI oc tack gee etc ace ieee brn Me enc os 4,726 FS TM re eT em gee ee ee 4,791 He Me Se a ae See ea eh Ee eure mene eet 5,096 TC OMMMNN ee Sty el hs ORE aaa acti nt ey SR) a ee 5,096 LOD 5 LS Se PINE eee oe een er 6,000 MONE MERC Ret cin ot), Ane nS SO ad a oak 9,000 FUQOR — 3c es ee ee pare ene see reece hie rae A Sei een 6,500 SOS May kee Gk Sy od teen ee 3,000 TSO ee ee ce Re ty Sr ye eek oN a 1,500 MSO eres as ee Gi ee Saal ae EE 1,500 MOOG eae ete ii eRe Mole Mic Tes On, 8 1,680 TUCO eas es epee RGEC pe rca cg i Mee eI a ee 322 TSO SMR Aces aa lela Ca era WER a INN Eee MUP Sos a aneeors 5,568 NOOO es aR ey eRe a oc Ta Se nO tN aby teat ee 18,280 MOO ORR aerate a eek Ge a MON ny, St 62,259 HOD) ao Bee esp ee ct Svea a Recs 137,259 SUGOE) 2G We re oe ree Re no eee von ee An ce ean 185,331 DOE, a cee a pe eae ae iar ie ean 554,286 MO OREM Ste er ia Guy ee ee uAn Gee ree ate ca oh 998,284 OO Mere aa eel icant dae Gaon an epee ite aia, 1,217.337 LOO 5 Ge Ua Ne ee ee auntie peta MME Da atten a 1,213,548 MOA eos gke ui iahiice SeGgt A onmiaan GRIER I hans Mie 3 820,844 OOS i, Sa ENA MI ath reer ia run ae nei er ee 727,767 HOW Opener or cutie ened ter aetna en eM NT Se a 639,016 MOM re ee ey aoe tate ae Da on 468,774 TUQ)ILTL A ae es as NEE A eer aga ine Ua bare re Uae ee 472,458 TOTO" 5 OG ce rg est eee ances eee RE UO oC ree 484,368 *Figures for the year 1922 and 1923 have been added.—Kd. 126 KENTUCKY ACADEMY OF SCIENCE, CTE a aainean Kiba tan em Me Tse RABE demons Rabe ae > 522,550 TOM ee Sahn. ase te pes See ee oe eee 479,609 OMS eer ei UN cli MCR ae 407,081 TONG MG Fes Fein Sle) en ae ernie hale ong tC eae 1,144,750 LOD ee ee eit ean ee ee ee Se a eee 3,015,640 nee Ge NERA ny aha ee EINE onic g . 4,035,950 HOMO bes Sin Oe cele selhnee nen cee hee ag hen tere ae 9,226,473 (020-2 ee ron nee ener ones ae 8,546,027 TOD re ee Nahe ee eer see ie SON ee cee 9,080,845 (Cp ley es RA OMe a tee eet ae ERS ME co arth. | _ 8,889,303 HOSS po eee erro a a eR a SA coe eas Oe ee *8, 087,250 In predicting the figures of crude oil production likely to be obtained in Kentucky in the future, estimates of certain and sure depletion now in evidence in many of the oil pools of this State, must be coupled with an unknown fraction representing oil produced from new pools now undiscovered. This latter factor is very much more difficult to come by than is an estimate of depletion which may be figured with some reasonable degree of precision. The price of crude oil is also an important factor in the ultimate amount of petroleum which will be produced in Ken- tucky. With a return at any time to extended low schedules for erude oil will come great demoralization in the producing industry in Kentucky, especially in the small and old well dis- tricts. On the other hand an increased price and a sustained demand for crude oil in the near future will tend to strengthen not only the entire industry as it now stands developed, but induce widespread ‘‘wild-catting’’. If the price of crude oil strengthens slightly and remains more or less stationary during the next several years, it seems reasonable to anticipate a pro- duction in Kentueky which will range between seven and nine million barrels, probably close to eight million barrels. The tenacity of some of the older wells in Kentucky, especially in the ‘‘true sand’’ districts of eastern Kentucky, is surely in- dicative of a somewhat more sustained production for these districts especially ; and in the end for the entire State, than had at first been assumed. Estimates of depletion for Kentucky made but a year ago, giving undue importance to the rapid decline of the ‘‘ Big Sinking”’ and associated pools, and assuming that Warren County had reached its peak must now, it appears, be revised in consideration of the new elements of long life in- *These figures supplied in 1924. Ed. NINTH ANNUAL MEETING. 127 troduced by the proving up of the characteristic of Eastern Kentucky pools. It is figured that the oil pools of Kentucky as now known and partly developed will still afford production through wells which are yet to be drilled, until the year 1980. For this period, 1922 to 1980 inclusive, it is figured that the known pools of Kentucky will produce 120,000,000 barrels of crude oil. Pools yet to be discovered in this State it is estimated will produce 80,000,000 barrels; a total of 200,000,000 barrels of petroleum now in the ground. A total volume of 44,655,145 barrels for the period 1883 to 1921 inclusive has been produced. The total production of petroleum from discovery in Kentucky from 1819 to date may be listed as follows: Petroleum produced in Kentucky. 1819 to 1882. inclusive (unmeasured, estimated) ....... 144,000 bbls. (Szeto 192t anclusive Cactual oil rums)in iss. . Ce c eee. 44,655,145 bbls. 1921 to 1980 inclusive (figured decline known)..... 120,000,000 bbls. 1922 to 1980 inclusive (estimated undiscovered new DIONNE MON) 5 So eigio do ole ¢ 80,000,000 bbls. otal petroleum, reserves 2 de 22-4: Bera cee seal 244,799,145 bbls. Of the above total of approximately 205,000,000 barrels, 44,799,145 barrels has been produced and consumed. The figured depletion of 120,000,000 barrels is regarded as conser- vative. It amounts to about 1,500,000 barrels annually until 1980. The estimate of 80,000,000 barrels of unknown, undis- covered oil in Kentucky is probably well within reason and probability for the period for 1922 to 1980, though it affords, it must be admitted, the greatest element of probable error in these calculations. With something over 200,000,000 barrels of petro- leum reasonably possible for this State, Kentucky’s place among the oil producing states of the Union and especially those of the Appalachian district seems assured. Oil Shales of Kentucky: C. 8S. Crouse, University of Ken- tucky. The oil consumption in the United States is outstripping the domestic production, creating an alarming situation. New sources of oil must be found. Oil shale will solve the problem so soon as the extraction of oil from this source is made com- 128 KENTUCKY ACADEMY OF SCIENCE. mercially feasible. A research has been in progress at the Uni- versity of Kentucky for three years with the development of a commercial retort as its object. The results are more than en- couraging. Kentucky has 90,000,000,000 tons of shale immed- lately available for steam shovel methods of mining. This shale, conservatively figured, represents 40,000,000,000 barreis of crude oil. Kentucky shales show marked superiority over shales in other parts of the United States. Such being true Kentucky is the logical place for the genesis of the oil shale industry in this country. Model Showing Structure of Gainesville Oil Pool, Allen County, Ky: E. 8. Perry, University of Kentucky. The author exhibited the model showing the stratification and explained its construction. Table Moving by So-called Spirits: Glanville Terrell, Uni- versity of Kentucky. An example of table-moving produced in daylight by a girl of fifteen and a boy of ten with no possibility of collusion, was described by the author, as having come under his observation, The author is convinced that the phenomenon was genuine but asserts his disbelief that it was a spiritual manifestation. A Kentucky Chemist of the Old School: Alfred M. Peter, University of Kentucky. (John) Lawrence Smith, M. D. (1818-1883), a citizen of Louisville, Ky., from 1854 to 1883, is most esteemed by the chemist engaged in mineral analysis by reason of the unique and very practical method for the determination of alkalies in silicates of his devising. Indeed, the extensive study of the potassium content of Kentucky soils, by the Ex- periment Station, was made practicable by the application of this method. Dr. Smith’s publications number some 150 titles, a large proportion of which appeared in the American Journal of Seience. His work was mainly in mineral chemistry. His in- vestigations on emery led to the development of the emery in- dustry in the United States. He made a life study of meteor- ites, of which he had a very fine collection, now owned by Har- vard University. Dr. Smith oceupied a high position in the TENTH ANNUAL MEETING, 29 scientific world and was an active member of many learned so- cieties both foreign and American, including the National -Acad- ~emy of Sciences. The Lawrence Smith medal of. the National Academy, a gold medal worth $200 to be awarded for re- search upon meteorites, was established by Dr. Smith’s widow, who used for the endowment the sum of $8,000 received from the sale of his collection of meteorites to Harvard University. The medal has been awarded only twice: to H. A. Newton, in 1888, and to Dr. Geo. P. Merrill, in 1922. Dr. Smith was a man of means, charitable, public spirited, always ready to contribute his scientific knowledge for public good, and was held in high esteem in the community. Home Economies as a Science: Margaret Whittemore, Uni- versity of Kentucky. Before considering home economics in relation to natural sciences it must be remembered that it has a vital connection also’ with social sciences and with the fine arts. For this reason, and also because it is distinctly an applied sub- ject, its relation with the natural sciences should be eniefly that of producer and consumer. Home economies, however, should contribute to scientific knowledge by suggesting problems which need attention and by providing the situations for application and experimentation. The earnest attenticn now being given to home economics reveals several weaknesses. One is the fact that as a course of study it has been organized too much upon a logi- eal in opposition to psychological basis. This seems still true of much of the teaching of the natural sciences, as shown by the requirement of inorganic before organic chemistry. Another cause of weakness is the failure to recognize the desirable limits of home economics and the frequent attempt to teach in the de- ‘partment the principles as well as the application of the arts and sciences involved. The Measurement of the Mental Changes after the Removal of Diseased Tonsils and Adenoids: Gladys Marie Lowe, Uni- versity of Kentucky. A group of thirty-five school children operated upon for diseased tonsils and adenoids was compared with a group of twenty-five which did not undergo the operation. ‘This study is 130 KENTUCKY ACADEMY OF SCIENCE, unique in the use of a control group of children with diseased tonsils and adenoids but not operated upon. Three lines of evidence were used, namely, changes revealed by a seale of tests of mental alertness, by the teacher’s estimate of certain traits, and by the actual scholarship records. The com- parisons are made between data obtained just preceding the operation and those obtained one year after the diagnosis. The Stanford Revision of the Binet-Simon Seale for measuring men- tal alertness was used. The teacher’s rating for each trait was obtained by estimating in which fifth of the class the pupils be- longed. The traits estimated were: (1) companionhsip with fel- lows, (2) emotional self control, (3) initiative, (4) self expression (speech), (5) interest in school work, (6) attention and (7) scholarship. The results show that: (1) While the average scholarship of the operated group continued to be the same as that of all the classes represented, the average scholarship of the non-operated group fell one scholarship rank below the av- erage of all the classes represented. (2) The operated group showed no more change in the mental age, or in “‘brightness”’ (I. Q.) than did the non-operated group. The differences com- pared with the error were so slight as to be negligible. (3) The teacher’s estimates showed no significant change. (4) Pro- nounced improvement was found in three of four cases. The Importance of Scientific Investigation in Marketing: O. B. Jesness, University of Kentucky. Attention was called to the growing complexity of market- ing methods and a comparison of present methods with the com- paratively simple methods that sufficed a century ago was made in order to suggest some of the reasons why the marketing sys- tem of to-day necessarily is involved. Mention was made of the prevalence of loose thinking and talking on marketing ques- tions. Emphasis was placed on the importance of scientifie in- vestigations in marketing. Facts are the only safe basis for action and careful studies are needed in order to obtain essen- tial facts. Agricultural experiment stations and departments have studied production problems for years but have taken up marketing activities only recently. Much work in this field is now being undertaken and the future should witness the ac- cumulation of much helpful material. TENTH ANNUAL MEETING. 13] Factors Involved in the Standardization of Tobacco Grades: Erle C. Vaughn, University of Kentucky. Standardization of tobacco grades is designed to avoid confusion, to stabilize prices, and _ to _ protect both producer and buyer. The factors involved are the conditions which must be considered in bringing about these results. The chief ones are: descriptive terms used, nat- ural grades, manufacturers’ grades, methods of buying and sell- ing, interest of producer in grading, and the many variaticns which occur both in the product and in opinions and practices concerning it. These factors, their true value and their rela- tion to each other must be carefully considered in establishing practicable standard grades of tobacco. Factors which Influence the Cost of Gain in Feeding Cattle: Wayland Rhoads, University of Kentucky. The foundation of the beef cattle industry is the production and sale of fat cattle for beef, so when cattle feeding: is profi- .table, both the breeders of purebreds and the producer of feeder steers have a good market for their stock. Pasture is the basis of the cheapest gains while the winter feed lot is necessary to produce fat cattle at that time, in order to have an even supply of beef. The cost of putting gains on eattle varies with a num- ber or a combination of things. They are the age of the cattle, the time of the year the cattle are fed, whether on grass or on dry feed, the length of the feeding period, the feeds fed, condi- tions under which the eattle are fed such as barn room and water supply, the daily gain which the cattle make, the quality of the cattle, the way they were fed before going on feed, the condition of the eattle and last the individual feeder himself. The old saying is true that ‘‘the eye of the master fatteneth the eattle’’. Geology and Eggs: G. Davis Buckner and J. H. Martin, Uni- versity of Kentucky. An experiment was discussed wherein two lots of ten White Leghorn hens each, all hatched the same day and coming from a common parent stock, were fed rations consisting of: No. ], corn, buttermilk and limestone, and No. 2, corn and buttermilk. During the first six months of laying lot No. 1 consumed 11.1 132 KENTUCKY ACADEMY OF SCIENCE. pounds of limestone and produced 651 eggs while No. 2 laid 343 eggs. Among other things it was shown that the average dried eggshell was 4.7 grams in lot 1 and 3.5 grams in lot 2. This means that lot 1 produced 1,789 grams of dried eggshell more than did lot 2. The relation of egg production to gecl- ogy may be inferred. Testing for Moisture in Transformer Oil: ©. C. Kiplinger, Mt. Union College, Alliance, Ohio. Freshly cut sodium dropped into the oil to be tested is a convenient and sensitive means of detecting traces of moisture. Evolution of gas bubbles is produced by smaller quantities of moisture than can be detected by the usual test of rubbing the oil with eosin. A Simple Apparatus for Demonstrating Heat of Absorption: C. C. Kiplinger, Mt. Union College, Alliance, Ohio. A Bunsen ice calorimeter with a long horizontal capillary tube attached serves as an air thermometer which may be used to demonstrate thermal changes due to absorption of liquids by charcoal. The Present Status of the Cancer Problems: (lecture) Dr. H. Gideon Wells, professor of pathology, University of Chicago. Many lantern slides, most of them from photomicrographs, were exhibited to show the nature of cancer, which may be de- seribed as the continued unnatural growth of cells. This growth euts off the supply of nourishment for other cells and makes conditions more favorable for the growth of bacteria around the affected area. It seems to have been established that the disease is not caused by a specific parasite, nor has a specific causative agent been isolated. Improved methods in the use cf radinm and X-rays seem to give the best promise for the control of can- cer, and great advances have been made in the last six years. Drugs are useless. A very important factor is eduzaticn of the people to recognize superficial cancers and have them treated before they become serious. There is no evidence that cancer is ever acquired by contagion either in man or in experimental animals. It appears to follow the Mendelian law of inheritance when studied in mice, resistance to cancer being dominant, sus- ceptibility being recessive. The application of these facts to TENTH ANNUAL MEETING. 133 human inheritance of susceptibility and resistance to cancer was discussed. A study of statistics shows that cancer is not on the increase, the apparent increase being accounted for largely by more exact diagnoses; the actual number of deaths caused by cancer is perhaps decreasing because of improved methods of treatment. ALFRED M. PETER, Secretary. The President then called for the reports of committees: Auditing Committee: Accepted the Treasurer’s statement as given showing a balance in the treasury of $100.34. Resolutions Committee : It is recommended. (1) That the technical papers be divided into two sections, to come after the business and general papers are presented ; one for the pure science and one for the applied science. (2) That the time schedule for the main divisions be part of the program. (Signed) HENRY MEIER, DANIEL J. HEALY, J. A. GUNTON. The Nominating Committee then reported: We, the undersigned committee, duly appointed and after consideration, desire to nominate the following persons for of- ficers of the Kentucky Academy for next year: For President, Mr. Lucien Beckner, For Vice-President, Dr. John A. Gunton, For Secretary, Dr. A. M. Peter, For Treasurer, Prof. W. S. Anderson. (Signed) FRANK T. MeFARLAND, Chairman. W. S. ANDERSON, “W. D. VALLEAU. The meeting then adjourned. (Signed) ALFRED M. PETER, Secretary. X. MINUTES OF THE TENTH ANNUAL MEETING The tenth annual meeting of the Kentucky Academy of 134 KENTUCKY ACADEMY OF SCIENCE, Science was ealled to order by President Lucien Beckner at 9:45 o’clock in the Physics lecture room, Universtiy of Ken- tucky, about 100 members and visitors being present. The Minutes of the last meeting were read and approved. The report of the Secretary was read and approved. SECRETARY’S REPORT, 1922-1923 Of the 15 persons nominated for active ntembershig at the last meeting, all but 2 have paid the initiation fee thereby adding 13 active members to the roll of the Academy, 11 national and 2 local. We have lost 2 members by death since the last meeting : Miss Cora Williams of Bellevue, Ky., and Mr. A. T. Parker, of Lexington, our oldest member. Two members have dropped from the list on account of removal from the state, J. B. Nelson and George H. Vansell. . The total membership is now 149, including 65 national mem- bers and 48 local, making 113 active members; 23 corresponding members and 13 honorary members. The membership may be classified as follows: Active members in good standing................ 89 Active.members in arrears for 1 year............ 12 Active members in arrears for two years.......... 12 Correspondinoe.memberse sae cic soe teste eee ieee 23 Honorary. anembers) Nahe. cyccelteis «1s are eves chs eecne menace 13 Members in arrears have been notified several times. The names of those who are 2 years in arrears after this meeting will be dropped automatically. Our members represent 38 lines of activity. About half belong to the group of physical sciences and about one-third to the biological sciences. Classified geographically and as to educational institutions our active membership includes: 50 from the University of Kentucky, Lexington. 7 from the University of Louisville, Louisville. 5 from Centre College, Danville. 4 from Georgetown College, Georgetown. 2 from the Eastern Kentucky State Normal School, Richmond. 2 from Berea College. Berea. TENTH ANNUAL MEETING. 135 2 from Transylvania College, Lexington. 1 from the Western State Normal School, Bowling Green. 1 from the Louisville College of Pharmacy, Louisville. 1 from Cumberland College, Wilhamsburg. 1 from Cardome, Georgetown. Not connected with educational institutions in the State are: 9 from Louisville. 5 from Lexington. 3 from Frankfort. and 1 each from Ashland, Wallins Creek, Harlan, Henderson. Winchester, Bowling Green, Newport, Jenkins and Carrollton. Total 26. Besides these there are 6 active members outside the State. Two new national members were elected since the last meet- ing by action of the council, viz., Mr. Ralph F. Schneider and Mr. Leonard P. Benjamin, both of the Public Service Laboratory of the Experiment Station. Their names will be presented by the Membership Committee to make their election entirely regular. In this connection I suggest that our constitution be amended so as to enable the council to elect new members in the interim between meetings of the Academy. This is for the benefit of any who may want to join as national members between the time of the Academy meeting and the end of the year, so as to be qualified to attend the December meeting of the A. A. A. 8. as members. Notice has been received from the Permanent Secretary of the A. A. A. S. in Washington of a change in the method of collecting the dues of national members. Beginning October 1, 1923, dues of national members will be paid directly to the Washingten office instead of to the Academy. The Permanent Secretary will remit monthly, to the Secretary of the Academy, so much of his collections as is due us; that is, one dollar out of every five, paid him as annual dues by our national members and all initiation fees ($5.00 each) paid by new national mem- bers. Under this arrangement, a newly elected member of this Academy who wants also to become a member of the A. A. A. 8. (national member) remits ten dollars to the Permanent Secre- tary of the A. A. A. S. covering initiation fee ($5) and annual 136 KENTUCKY ACADEMY OF SCIENCE. dues ($5) for one year in advance, beginning October 1st. The Permanent Secretary records the new member as being fully paid for one year, enters his subscription for the journal and, in due time, remits six dollars to the Secretary of the Academy. Two dollars of the six the Academy claims; one for the new member’s initiation fee and one for one year’s dues, in advance. The remaining four dollars your Secretary regards as a credit due the new member, to be applied according to that member’s wishes, unless the Academy instructs otherwise. Secretary Liv- ingston’s letter announcing the new arrangement is made part of this report. I wish to call the attention of the Academy to the annual meeting of the A. A. A. S. to be held in Cincinnati beginning December 27, 1923. This is an unusual opportunity for oar Kentuckians to participate in this important gathering of scien- tists and every member of the Kentucky Academy should make an effort to attend at least part of the time. It has been sug- gested that we hold an extra meeting of the Academy at that time, in Cincinnati. The President appointed Dr. J. J. Tigert to represent the Academy at the Spencer F. Baird Memorial Celebration in Washington on February 3rd last. annualmMeeting :.50,3c ets. 1s) sie saeie, = sieloseraels cata euselaterem eee enone 57 First food of young black bass, Garman...-...........++scsseeee 110 Burst SIC CIN Orta rslneoredeersials: slcadelevauecelecaleke Nice) diavtyel ceaycoearadn oleae aera 23 Hishsremaimse@Devoniam), WAl Mi; Maller... : cay. tals ares creteetenegs meres 7 Food value of. certain insects, McHargue...................6---- 46 Hormation,of petroleum, Norwood ...25 3:0, 2 selec siesit wie aoe le gel eoreyeneeees 62 Bourtho annuals ime etim oo. seeks wins cape sare ap cuelsie austere Magee eee eee 42 Hrequency, meter, NiORs Smithis 22 ae oa eres eee sao oeen eee 38 Frost and length of growing season in Kentucky, Walz.......... 48 Frost injury to some trees in the Bluegrass region, Hemmenway.. 140 Future of nutrition and medicine, Homberger Se eee a ORT boIcioicny O18 o 78 Hunioicot Kentucky, IMicharlands ce sicc.cec a «smi ol -\-ste secvelenensyetslencusieneise 63 Fungi of the Kentucky mountains, G. D. Smith.................. 84 Funkhouser, W. D., Orthogenesis in the membracidae............ 109 Huturevof the. Kentucky Academy, Boyd... 6.0. << cccsslteloncitoremerente 76 Galvanometer, an improved astatic, Kiplinger................... 70 Gardner J. H.; Kentucky. as anol state... (2.0. iacls ae ieee ee 48 Garman; H.,, A) little-known) Cave Crayfish... o/s. acatenotelener eeenaees 87 Garman, H., A new phyllopod crustacean from Kentucky.......... 85 Garman, J. -H., First: food of youns, black: bass. 2..5- cenit ee 110 Geology Of eros, TBUCKmer ys ciccte ie eG rcsites a soi ae ler ole etalon le dle nem ren Ree ee rane aril Geologic structure in the Irvine oil fields, x M. Millers 2. eorisis 47 Germination of sclerotia of claviceps, McFarland: .:...0ssoss0ce 119 Gilbert eAw He sotem roteod call hela cs atta scree caencrse eee eee 38 Good, E. S., Etiology of infectious abortion of live stock.......... 94 Gott, E. J., and Brown, Market milk free from B. coli............ 141 Graph of an equation in which variables may be separated, Rees... 63 Growing seedlings in test-tubes with filter-paper and water, Didlake 111 INDEX Gunton, J. A., Hydroxyanthraquinone derivatives in plants........ Haddix-Coalburg geosyncline, Jillson ..............- eee eee eee ee Hart, E. B., The widening viewpoint in animal nutrition..... euaare Healy, D. J., Bacteriological descriptive group number............ Healy, D. J., H-ion concentration and biological reactions.......... HealyeD J. Lolerance ot hogs: for arsenic.:.t.2..- ites eite ete Hemmenway, A. F., Late frost injury to some trees in the Bluegrass ME CUON Ms pysmye cetyige ce Un aRe eet ea Snea er ateds ohe aN apaslner slang terauelsa ciel olsseleettals H-ion concentration and biological reactions, Healy.............. Holmes, P. K., Inefficiency of the efficiency expert............... Homberger, A. W., The future of nutrition and medicine.......... Home economics as a science, Whittemore....................-- Hooper, J. J., Effect of developing fetus on production of milk of lasiltayaa COWS anemone y cai’ sree euatorsrsiceosi cnete ores mista retaen ontzinn ie cette ene eenteee Hooper, J. J., Inheritance of coat colors in Jersey cattle.......... Hydnacedveronewentuckiys (Gers smiths a cieces, aie eee cleustcaseoicdeneneliciene cits Hydroxyanthraquinone derivatives in plants, Gunton............ Inefficiency of the efficiency expert, Holmes.................0.4. Infectious abortion of live stock, etiology of, Good.............. Inheritance of coat colors in Jersey cattle, Hooper............... Interspecies crosses in the genus Nicotiana, Routt................. Jeptha Knobs of Shelby County, Bucher... 0 ioc. setes oe ene Jesness, O. B., Importance of scientific investigation in marketing. . JUS Omer A vOlganticcslate ‘Sldeicg. aces sleet cieuctneetatere teers one Jilison, W. R., A mica deposit in eastern Kentucky.............. Spill Som wa saukvss A Sp baltic Oailecc ie o's sate asceiee a, spade sete alehenesetepeasloe sare Jillson, W. R., Cretaceous sediments between the Cumberland and Tennessee rivers in western Kentucky...................0.- Jillson, W. R., Depletion of Kentucky crude oils................. Jnlison Wek. iuand, of ten thousand: isimks. oe iien ee sete e dus ee soerees Jillson, W. R., New fossil invertebrates from the Coal Measures of eels be mmbesee mit Clyiscctsmeee ec rerzeat oni seitaceatiren sliaceuinepatatatale ieciraleliet Om tacts Jillson, W. R., The Haddix-Coalburg geosyneline.................. Kastle; J. H., Work of the Experiment Station.................. Kastle, J. H., Recent advances in knowledge of animal nutrition.. Kentuckysas, anol state; iGatdner:. aa cacic. 0. veueecigene Gate ss ures Kentucky chemist of the old school, A. M. Peter.................. Kentucky petroleum problems, Beckner.................:...-2-- GAS ater CE VOM sD Aye sec ees eucsenares sscuern oeouake tanec ofictoy soasal suv tetn ahenaeenneey aes Kiplinger, C. C., A modified ebullioscopic apparatus.............. Kiplinger, C. C., An improved astatic galeanometer.............. Kiplinger, C. C., Cryoscopiec work with an ordinary thermometer.. Kaplinger, ©. C., Notes on, light. and light pressure............:. Kiplinger, C. C., Notes on the constitution of benzene............ Kiplinger, C. C., Simple apparatus for demonstrating heat of ab- SMONGT EM OMe ary ayarce cas vais gvitesce Stour tos ances Caney basement arnumstcace taaeliate hier e /aeicdas atage Kiplinger, C. C., Testing for moisture in transformer oil.......... WandHote ten thousands simi SOM ieee on cess leu tsses cates lees wees lasthwarnine of thesrattler: Ga Di iSmithieia <5 oc ce is silent ce ee ieoislatuve committee, erphth= meeting ic cs) sists oe eter ioe acim oe Lettuce, failure to head, Olney and Valleau....................6. Michens of Cowbell Hollow, G. D.-Smith.... 20... . cece ee cc wees iiohteands lish tapress umes. sharp limo eM year) ss ene ucts cssteiniee) seers sae cee wodestonemtromuken tuck, sR et ete acscpepicveene ties eteccu-uavaleke ssc ches chee Lowe, G. M., Mental changes after removal of diseased tonsils and ACeIOMM Sei reiraicecmasiapecs ten essere, saci al ousventetiy earn ala shucs: oka emeneraraacee Magnesian limestone, rapid analysis of, Averitt.................. Maenolia fraseri; does it occur in Kentucky? MecFarland.......... 154 KENTUCKY ACADEMY OF SCIENCE Page Manganese and the growth of wheat, McHargue.................. 62 Manganese iand= vitamins: Micklanrome: sce.) se cine eee 140 Manganese. in plants; McHarouess any. «ci. ss 2 seriteeret eta 120 Manning, Van H., Work of the Bureau of Mines.................. 24 Market milk free from B. coli, Gott and Brown........-.s2. 6: 141 Marketing, importances of scientific investigation in, Jesness...... 130 Martin, Dean W., Electrolytic solution. glow:-......- cca scene 68 Martin, J. Holmes, Animal vs. vegetable proteins in the ration of ayaa PW CMs 2.55 sipcke eae Cisne tte iene isretetol siecle ori cegeren ee aer 96 Matenanprinma. Vian: Becelaere wen cssaseictee cose kei ern 84 Matter and sradiant energy. N). Smith. . 2.0 see eee eee 28 Mean- value theorem, =D ownine’ -ls. 20> a1.c0s «ores <4 eee eee 63 MeversclennryasAs tronomuy applied ss ss. sss ie oe See eee 37 Meier, Henry, Deductions from places of comet B., 116........... 46 Meier, Henry, Extraction of petroleum by means of shafts and GAUTVTVICIS stress eapeegs Sencatey dees settee, abe ons reeai bi Caeenalrenewele etc ue ce Cece ca ara 122 Meier, Henry, Extraction of petroleum by means of shafts and cate with supposed inhabitants of Mars............:.....:.. UL Members hap cM OWAas) Bis oi) Vos eso oitca conc asusial cacao etc ee RSI 15 MiemionyarsivSit ems avialuem- Ot amMio erties ic: \ces coccste a aees oe lense Mea Ie 84 Mental and physical correlation, experiment in, Tigert............ 109 Mental changes after removal of diseased tonsils and adenoids, TS OMS ae errr hereMeot hors. c aacie Bocoaesvejangcscns tar ocseonePateraiers ere aah ae ee ee 129 McFarland, F. T., Factors affecting the germination of the sclerotia OP RACALVTC EDS) adi hace csr eta cesdh ot dhosaels sete oe eee aR RE ee 119 MeMarland; HT. list: of fungi from Kentucky... 40. ss oeeeaee 63 MeFarland, F. T., Magnolia fraseri; does it occur in Kentucky?.... 63 McFarland, F. T., Occurrence of two fern rusts in Kentucky....... 139 Micharland, i Io The Robert Peter herbarium....)..22n eee 38 McHargue, J. S., Association of Mn with so-called vitamines..... 140 McHargue, J. S., Effect of Mn on the growth of wheat.......... 62 MecHargue, J. 'S., Food value of certain insects..........55..28005 46 MeHargue, J. S., Mineral constitutents of the seeds of ocklebur.. 95 McHargue, J.8., Plant-food in drainage waters................... 30 Meklaroues dS. The role of Mn inviplamts\.. sce .c4 sarees 120 Mica deposit: im eastern Kentucky, Jillson.......% 1. oss sseaeeuaee (fal Maller, A’ M., Faulting in north-central Kentucky....:....4..5.04 29 Miller, A. M., Historic fish remains (Devonian).................. 37 Miller, A. M., Geologic structure in the Irvine oil field.......... AT Muller, “As MM... McCreary. County Acrolite.. <... 0.4.0 eee val Muller Aes Mi -Orioim: and. antioqultya cessor ces cteeein Gre eaters Wal nets cs ene sey Seed-borne diseases of crops, Valleau..............00 cece eee eeees Seed-corn situation in Kentucky, Valleau...............00.00e0 ee Seed infection in supposedly disease-free corn, Valleau.......... 156 KENTUCKY ACADEMY OF SCIENCE Page Seventhisann wal smie et im or css vi-i uous sy ceasneienel aval ctescllet ile civeiey oe ehyeerencneeets 72 Sewage purification at Lexington, Speers................---e-+-- 69 Shull CsA: Adsorption inet hevcorneyoraimy.) 3.c.y1-0s ie) cl-teroineter enon 109 Shull, C. A., Variation in abutilon theophrasti................... UC SPX Ges ANU AEs e CLL TUG ry aretesohs ves kate ease sei encied= eileucy viele ofetslisrecoremenenonteeeaemeMente 65 Slates slide; a7 Si gamtacss dill SOM spatres.sei evcuers! stare siesieastetenie sterner temenoe 146 Smith, G. D., Fungi of the Kentucky mountains, Lichens of Cowbell FAOULO Wesiee. PeNsete war cucisetisse totals even acle avelaee in crabeneusveNeiee sl enema Olea teeaee eee 84 Smitha GsD The Bolet of Kentucky. oes ccm deer cee errs 119 Smith: G. 0. | AON ee cer a ie a ee 172 Classed geographically and as to educational institu- tions our active membership includes: Or TS from the University of Kentucky, Lexington. from the University of Louisville, Louisville. from Centre College, Danville. from Berea College, Berea. from Eastern State Normal School, Richmond. from Western State Normal School, Bowling Green. from Transylvania College, Lexington. from Georgetown College, Georgetown. from the College for Women, Danville. FPmowwwwnraso Not connected with educational institutions in the state are: Nine from Louisville, 7 from Frankfort, 4 from Lex- ington, 2 from Ashland, and 1 each from Shively, Wallins TWELFTH ANNUAL MEETING wal Creek, Danville, Mayfield, McAfee, Stearns, Versailles, Ky- rock, Winchester, Harlan, Jenkins and Middlesboro. Be- sides these there are 7 active members outside the state. The Council of the A. A. A. S. at their annual meeting in Washington voted to decrease the refund allowed to af- filiated Academies to 50 cents for each national member, instead of $1.00, beginning October 1st, next. In view of this change the Council of the Academy proposes an amend- ment to our constitution whereby the dues of our national members will be increased 50 cents, making national mem- bers pay $2.00 a year to the local Academy instead of $1.50. The proposed amendment was sent to the membership with the announcement of the present meeting, in order that it might be acted upon at this meeting. The President appointed the following Membership Committee: W. G. Burroughs, Chairman, 8. I. Kornhauser, and V. F. Payne. Also a committee on arrangement of pro- gram for this meeting composed of A. M. Peter, Chairman, Henry Meier, Robert T. Hinton, Harry Best and P. P. Boyd. This committee met at the Phoenix Hotel on November 1st, 1924, and prepared a report to the Council. This report was adopted at a meeting of the Council on March 27th, 1925. (See copy attached). It has been followed in arranging the program. The Council held two meetings during the year, on March 27, and May 15, 1925, the minutes of which are at- tached to this report. The program of our last meeting, with a short account of the meeting, was published in “Science” under date of June 13, 1924. Volume 1, of Transactions, covering the first 10 meet- ings of the Academy, was mailed to the membership shortly after the last meeting, each member receiving one copy free. Extra copies will be sold to members at actual cost, $1.25 per volume. We have about 50 of these volumes left. The printing has been paid for, Dr. Jillson having assumed $242. of the amount, the Academy paying the balance of $174.23. 92 THE KENTUCKY ACADEMY OF SCIENCE The Secretary received the following letter from Wil- liam S. Cooper, Chairman of Glacier Bay Committee, of the Ecological Society of America, in regard to Glacier Bay, which he promptly acknowledged. “You will be glad to learn that on February twenty-sixth President Coolidge issued a proclama- tion establishing the Glacier Bay National Monu- ment in Alaska. On behalf of the Ecological So- ciety of America, which has had the movement in charge, I wish to express sincere appreciation of vour valued assistance in the campaign.” Our Academy endorsed this movement at the last meet- ing. The American Forestry Association, thru its executive secretary, Mr. Ovid M. Butler, Lenox Building, Washington, has asked the endorsement of the Academy for the acquisi- tion of forest land by the Federal government, under the Clarke-McNary act. The movement has been endorsed by Section O (agriculture) of the A. A. A. S., and seems worthy of our support. Your endorsement has been asked, also, by the Committee on Preservation of Natural Conditions of the Ecological Society of America for the project to pre- serve the Cook Forest in northern Pennsylvania. A cordial invitation has been extended to this Academy by the Indiana Academy of Science, to join their field meet- ing at Madison, on May 14, 15, and 16. Your Secretary ack- nowledged the invitation with thanks and the explanation that we could not change the plans already made for our annual meeting, but that individual members are free to attend. On request of the management, your Secretary named Dr. G. Davis Buckner to represent the Academy on the gen- eral committee of arrangement for the sesqui-centennial celebration of Lexington. Dr. Buckner has attended all meetings. Respectfully submitted, A. M. PETER, Secretary. TWELFTH ANNUAL MEETING 93 Mare bi 925. The following papers were presented at the Twelfth Annual Meeting of the Academy: (1) Caleium Metabolism in the Laying Hen. G. Davis Buckner, J. Holmes Martin and A. M. Peter. We have shown experimentally that a calcium deficiency in a diet abounding in protein was not the primary cause of the production of shell-less eggs but that it caused a gen- eral depletion of the body material of laying hens, especially the bones, and a general lowering of the vitality of the hens. We have further shown that unless the ordinary food stuffs fed to laying hens are properly supplemented by some mat- erial which is high in calcium carbonate, such as cracked oystershell or limestone, there will be a gradual cessation of egg production, accompanied by a thinning of the egg shell. Again we have shown that calcium carbonate greatly increased the efficiency of buttermilk for egg production by increasing the number of eggs laid and causing the produc- tion of eggs that had heavier shells and a larger edible part. The experiment described in this paper was undertaken to determine how such increase in weight of the edible part of the egg was distributed between the yolks and whites and whether the protein and calcium content of the whites and yolks were changed. With this idea in view, three lots, each containing 10 white Leghorn hens were selected having egg records for the pullet years ranging from 153 to 170 eggs. These hens were the same age, 19 months, and were grouped so as to make the lots as nearly equal as possible in weight, vigor and egg production. Lot No. 1 was allowed a fair sized grass range and lots 2 and 3 were confined to their houses during the experiment with ample direct sunlight available. A trapnest record was kept thruout the experiment and the eggs were weighed. The yolks and whites of the eggs 94 THE KENTUCKY ACADEMY OF SCIENCE were separated, weighed separately, and nitrogen and cal- cium were determined in each portion. All three lots were given buttermilk ad libitum with a grain mixture of wheat and yellow corn and all received some green food such as lettuce, cabbage and kale twice a week. An oat straw litter was used and sufficient grit that was free from available calcium was supplied. Crushed oystershell was supplied ad libitum from Nov. 1st to May 1st to lots 1 and 2 and withheld from lot 3. Beginning May 1st and continuing 3 months the oystershell was withheld from lots 1 and 2 and given ad libitum to lot 3. To summarize briefly the results obtained we can say that the total edible part, yolks and whites, of eggs pro- duced by the hens that received oystershell during the first 6 months weighed distinctly more than those produced by the corresponding hens that had not received calcium car- bonate. When oystershell was added to the diet of the hens that had not received it for 6 months, the weight of the contents, whites and yolks, was not increased, but the number of eges per hen was trebled. When oystershell was withheld from the diet of the hens that had received it for 6 months, the total content, yolk and white, weighed distinctly less than before. When oystershell was withheld from the diet of the hens that had received it for 6 months, the number of eggs produced per hen was halved in the pen confined to the house but was not effected in the pen that had daily range. The presence or absence of oystershell in the diet had no appreciable effect upon the percentages of protein and of calcium in the whites and yolks. From a practical standpoint it will be seen that the addition of a calcium supplement such as oystershell to a grain, buttermilk diet, causes an egg to be produced whose edible portion is approximately 10 per cent larger, besides increasing the number of eggs laid three-fold. TWELFTH ANNUAL MEETING 95 (2) Lactobacillus Acidophilus vs Lactobacillus Bul- garicus. M. Scherago, Head of Department of Bacteriology, University of Kentucky. The reports of recent investigations, that the alleged beneficial effects on the intestinal tract which Metchnikoff attributed to Lactobacillus bulgaricus are in reality due to an implantation and proliferation of Lactobacillus acidophilus, have stimulated renewed interest in the comparative study of these two aciduric organisms. Any one working with Lactobacillus acidophilus and Lactobacillus bulgaricus is soon impressed with their marked similarity in morphology and in cultural characteristics and wonders at the differences displayed by these two strikingly similar organisms in their ability to implant themselves in the intestinal tract. When Bouchard announced his theory of intestinal in- toxication in 1884, many diseases and conditions were treated with the idea of overcoming intestinal putrefaction by means of various so-called antiseptics. In 1907 Metchnikoff pub- lished his book ‘‘Prolongation of Life’ in which he claimed that auto-intoxication and premature senescence were brought about by the absorption of the products of putre- factive bacteria from the intestinal tract. Metchnikoff pro- posed to combat these harmful toxin-producing micro-organ- isms by crowding them out by the ingestion of large num- bers of harmless bacteria and used for that purpose milk soured with Lactobacillus bulgaricus. He decided to use this organism because he believed it to be the predomnant one in the milk and milk products used by the famously long-lived natives of Bulgaria. It is interesting to note that since the first appearance of Metchnikoff’s book the greatest interest has been shown in the prevention of the onset of old age by changing the intestinal flora, as manifested by the appearance on the market of all kinds of preparations of Lactobacillus bulgaricus in the form of tablets, capsules, liquid cultures and even in the form of Bulgarian Milk, and these preparations were used with more or less enthusiasm by practically all physicians. 96 THE KENTUCKY ACADEMY OF SCIENCE Altho several observers, including Leva, Cohendy and Belonowsky, at first reported the presence of Lactobacillus bulgaricus in the feces of persons who had ingested Lac- tobacillus bulgaricus milk, later work failed to substantiate Metchnikoff’s claims. Among the first to demonstrate that Lactobacillus bulgaricus cannot grow and be colonized in the intestinal tract were Herter and Kendall. By killing a monkey three and a half hours after it had been fed 500cc of milk soured with Lactobacillus bulgaricus, they found that the organism did not survive below the ileocecal valve. In an earlier experiment with the same animal they failed to detect Lactobacillus bulgaricus in the feces after feeding the sour milk for three days. Distaso and Schiller, and Rettger and his associates have also shown independently that the implantation of Lactobacillus bulgaricus cannot be brought about in the intestines of the white rat by the ingestion of this organism even when taken in enormous numbers. In a more recent extensive investigation Rettger and Cheplin have definitely proved that Lactobacillus bul- garicus cannot be implanted in the intestinal tract of either white rats or man. After feeding large quantities of this organism to several rats for a week or more, in no instance could the organism be found in the feces; and on autopsy none were found in cultures made from various portions of the intestine 24 hours after the last feeding. They also failed to isolate the organism in a single instance from the feces of human beings who had previously ingested large quantities of milk soured with Lactobacillus bulgaricus and had taken the milk over long periods of time. Metchnikoff, in his search for a harmless organism, at- tempted to acclimate a lactic acid organism to such an un- natural environment as the alimentary canal. Furthermore, he evidently overlooked the important influence of diet on the predominating type of bacteria in the intestines. It is now well known that there is a definite relation between the character of the food and the type of intestinal flora. Herter and Kendall first noted this in 1909 when, by substi-- TWELFTH ANNUAL MEETING 97 tuting a diet of milk and sugar for meat and eggs, they were able to convert a proteolytic putrefactive bacterial flora in the intestinal canal into an aciduric non-proteolytic type. In other words, the intestinal canal acts as an ideal culture tube in which certain organisms thrive best in media con- sisting of large amounts of carbohydrates, while others thrive best in media containing large amounts of animal protein. Asa result of the proteolytic action of the putre- factive bacteria, toxic substances are formed, which are as- sumed to be the cause of the various forms of intestinal tox- emia; and in addition, considerable amounts of gas are pro- duced. Combe, Herter and others have shown that the fecal flora of the adult consists almost always of putrefactive bac- teria which are therefore potential disease producers. Pro- teus, Cl. putrificus, Cl. welchii and E. coli, all of which are normal inhabitants of the human intestinal tract, have been shown by various investigators to have at times been the cause of various types of diarrhea, cystitis, cholecystitis and intestinal poisoning. This predominance of putrefactive bacteria in the adult is however dependent upon the con- tinued ingestion of a proteinaceous diet. For, as Rettger and Cheplin, and others have pointed out, if we change from a proteinaceous to a carbohydrate diet the intestinal flora changes from a proteolytic type to an aciduric type. Tissier, in 1900, demonstrated that the meconium of the new born baby is sterile, but if it is breast fed exclusively there is established in a few days an intestinal flora con- sisting almost exclusively of B. bifidus. This organism re- mains predominant in the intestinal tract as long as the infant is breast fed and can be obtained in almost pure cul- ture from the feces. In 1900 Moro described another acid- producing organism which is also found in the intestinal flora of nursing infants. He named this organism Bacillus acidophilus and claimed that it was the predominating form; but after closer study accepted the claim of Tissier that B. bifidus is the predominating organism in the intestines of nursing infants. As the infant is given other food the num- 98 THE KENTUCKY ACADEMY OF SCIENCE ber of B. bifidus organisms gradually decreases and there is a corresponding increase in the number of Lactobacillus acidophilus organisms so that by the time that breast feed- ing is discontinued the intestinal flora consists almost en- tirely of Lactobacillus acidophilus. This organism remains predominant for about a year or two after breast feeding is discontinued and then the flora gradually becomes more complex as the diet becomes more complex so that even before adult age is reached the simple aciduric flora is re- placed by a great variety of other types that become estab- lished. In adults only a few, if any, acidophilus organisms can be found in the intestinal flora. It is evident, then, that diet has a marked influence upon the bacterial flora of the intestines and, therefore, the simple aciduric type of bac- teria as represented by the B. bifidus and Lactobacillus acid- ophilus in the baby gradually gives way to the more complex proteolytic and putrefactive type as the diet of the baby gradually approaches that of the adult. If diet plays such an important part in influencing the type of intestinal flora, is it possible, by proper feeding to change the intestinal flora? Attention has already been called to the work of Herter and Kendall and that of Rett- ger and Cheplin. The latter found that they could trans- form the intestinal flora of white rats from the gas forming putrefactive type to the Lactobacillus acidophilus type by feeding them in addition to their daily diet two grams of either lactose or dextrin. They began to notice an increase of Lactobacillus acidophilus within two days and the maxi- mum implantation took place within four to eight days. They also fed these sugars to men and found that whenever they fed 300 grams or more per day they produced a change in the intestinal flora within four to eight days, the putre- factive bacteria having practically disappeared and the Lac- tobacillus acidophilus constituting the most predominant organism present. Smaller quantities of sugar would bring about an increase in the number of Lactobacillus organisms but there was not an appreciable decrease in the number TWELFTH ANNUAL MEETING ys) of the other bacteria present, especially the gas formers. Bass’ experiments substantiate these results and indicate that the amount of sugar necessary to bring about a trans- formation in man is 300 to 450 grams per day, a quantity that can hardly be continued over long periods of time and therefore a limiting factor in its general usefulness for thera- peutic purposes. : Rettger and Cheplin in 1919 resorted to the use of cul- tures of an aciduric organism, Lactobacillus acidophilus, with the expectation that these organisms would remain in the intestinal tract and colonize there as they do normally in the intestines of babies. They fed broth cultures of Lac- tobacillus acidophilus to white rats, in addition to their daily diet, and found that when they fed a sufficient quan- tity the fecal flora changed rapidly to the Lactobacillus acido- philus type. They obtained similar results in man when they fed sufficiently large quantities of the broth culture. Early in 1920 Rettger and Cheplin substituted for the whey broth cultures, milk cultures of Lactobacillus acidophilus because, as they put it, ‘acidophilus milk is much to be pre- ferred to the lactose broth or whey broth cultures of the or- ganism for the following reasons: the milk is tolerated by those who are unable to retain the simplest and most whole- some foods for convalescents; when properly prepared and preserved the acidophilus milk remains practically un- changed and free from bacterial contamination and deterio- ration; it contains at least four per cent lactose which in itself serves to stimulate Lactobacillus acidophilus prolifera- tion in the intestines; it is nutritious, and for those who cannot take or do not tolerate other foods it does much toward the maintenance of nitrogen balance and the pre- vention of tissue waste, when taken in the usual amounts, from one pint to one quart daily, and finally,.as a young culture of viable bacteria it is particularly potent in bring- ing about the desired transformation of bacterial types in the intestine.” Their first experiments were performed upon animals and apparently normal human beings and their con- 100 THE KENTUCKY ACADEMY OF SCIENCE clusions suggested that the simplification of the fecal flora might possibly be of benefit in certain pathologic cases in which there is associated a predominance of the putrefactive forms of bacteria in the intestinal tract. They applied their Lactobacillus acidophilus milk therapy to more than 60 cases and reported that the ingestion of Lactobacillus acidophilus milk resulted in relief from chronic constipation, chronic diarrhea, mucous colitis and sprue. They also reported bene- ficial results in the treatment of eczema referable directly to the bacteriology of the intestinal tract. Kopeloff and Cheney also reported good results from the use of acidophi- lus milk in seven cases of marked constipation occurring in patients with various psychoses, in four normal patients with mild constipation and in two patients with inter- mittent diarrhea. Kopeloff has also shown that relief from constipation has persisted for six months after the ingestion of Bacillus acidophilus has been discontinued. A more re- cent communication from Kopeloff reports good results with Bacillus acidophilus milk in thirty constipated cases and two cases of diarrhea. The constipated cases were observed from a week to about a year after treatment, and almost without exception all had more normal defecations than be- fore treatment. Gompertz and Vorhaus in their experiments with 200 cases of chronic constipation and 100 cases of diarrhea and mucous colitis were very favorably impressed with the results, 70 per cent of all the cases of either group showing complete relief from symptoms and toxemia and 15 per cent showing some relief and improvement. Mizell, experimenting with 20 patients, reports that ‘Relief from chronic intestinal toxemia, putrefactive type, and _ stasis, even in the presence of intestinal adhesions, has been se- cured while feeding acidophilus milk in conjunction with a general diet; and that chronic ileo-colon and colon stasis of undetermined origin as well as secondary to intestinal adhesions may be relieved by feeding acidophilus milk.’ Bass and Norman and Eggston have also given the acido- philus therapy widespread application and strongly advo- TWELFTH ANNUAL MEETING 101 cate the acidophilization of the intestinal tract by means of milk cultures. Experiments to determine the therapeutic value of Lac- tobacillus acidophilus milk cultures were carried out at the University of Kentucky on 40 individuals who had the fol- lowing conditions as diagosed by their respective physi- cians: Chronic constipation, 20 cases; Chronic colitis, 2 cases; Debility and anemia, 4 cases; Chronic diarrhea, 2 cases; Rheumatism, 1 case; Dermatitis and acne, 5 cases; Intestinal toxemia in children, 6 cases. The amount of acidophilus milk taken per day and the duration of the treat- ment varied with the age and the condition of the patient. In every case the patient and his physician reported marked improvement. One of the patients with chronic constipa- tion, who was an epileptic, reported fewer attacks during his period of treatment than ever before within a similar period. It is intended to carry out experiments on a larger number of epileptics to see if a transformation of the in- testinal flora has any effect on the number of attacks. The therapeutic value of Lactobacillus acidophilus, be- cause of its ability to colonize in the intestinal tract and displace the putrefactive bacteria, is definitely established. The instances of presumably successful implanations with Lactobacillus bulgaricus all show long periods of feeding of large quantities of milk soured with this organism. Such soured milks contain as much as four per cent lactose which in itself would bring about a proliferation of Lactobacillus acidophilus in the intestine if fed over a long period of time. It is not surprising then, because of the close resemblance of the two organisms, that Metchnikoff and his followers thought they were getting an implanation of Lactobacillus bulgaricus when in reality the organism that they observed in the feces after the feeding of milk soured with Lactobacil- lus bulgaricus was not this organism, but Lactobacillus acidophilus. The close resemblance of Lactobacillus acidophilus and Lactobacillus bulgaricus, both morphologically and cultur- 102 THE KENTUCKY ACADEMY OF SCIENCE ally, makes differentiation between these two organisms ex- ceedingly difficult. As a matter of fact this similarity has caused some investigators to conclude that the two are iden- tical. A separation of the two species is based largely on. their action upon maltose, sucrose and levulose. Most strains of Lactobacillus acidophilus ferment these three sugars while most strains of Lactobacillus bulgaricus do not attack them. Lactobacillus bulgaricus being more variable in this respect than Lactobacillus acidophilus. Recently Albus and Holm have been able to differentiate the two species on the basis of surface tension using sodium ricinoleate and sodium tau- rocholate as surface tension depressants. They found that all of their fifteen strains of Lactobacillus acidophilus grew very well in a medium having a surface tension as low as 36 dynes, while a similar number of strains of Lactobacillus bulgaricus in the same medium depressed to a surface ten- sion of 40 dynes, failed to show growth after seven days in- cubation at 37°C. They conclude that a surface tension of 40 dynes represents the extreme lower limit for Lactobacil- lus bulgaricus. Kopeloff and Beerman state that they have good reason to believe that the enzymes of Lactobacillus acidophilus differ from those of Lactobacillus bulgaricus. Just why two organisms so strikingly alike in all other aspects should manifest such marked diversity in their be- havior in the alimentary canal has been puzzling. It may be that the enzymes of the digestive tract have a differen- tial action upon the two species. Albus and Holm suggest that surface tension may be a factor in the implantation of these organisms. However, this does not explain the failure to recover any Lactobacillus bulgaricus organisms in feces after feeding milk soured with this organism. It is possible that a specific bacteriophage, lytic for Lactobacillus bul- garicus and not for Lactobacillus acidophilus may be the important factor. This problem is at present under con- sideration. TWHLETH ANNUAL MEETING 103 (3) The Asscciation of Copper with Substances Con- taining the Fat-Soluble A Vitamin. J. S. McHargue. (Abstract) Small amounts of copper are widely distri- buted in rocks, soils, waters, plants and animals. A method for its estimation in tissues of plants and animals is des- cribed. Copper occurs in the largest amounts in the young and tender leaves and in the germs of the seed of plants. It is a normal constituent of the blood and tissues of mam- mals and occurs in greatest concentration in the offspring previous to and at the time of their birth. Colostrum is relatively rich in copper and normal milk contains appre- ciable amounts. It occurs in the fat, and most of that car- ried in normal milk is separated with the cream. Upon churning, the copper remains in the butter. On heating the butter, curd is precipitated and carries with it considerable copper. The occurrence of copper in greatest concentration in some of the mosi vital organs of plants and animals leads the author to infer important biological functions for this element in the normal metabolism of plants and animals. (For the complete paper, see American Journal of Physio- logy, Vol. LX XII May, 1925, pp 583-594). (4) Anaphylaxis and Hydrogen Ion Concentration. Daniel J. Healy, Howell Spears and Louise B. Healy, From the Laboratory of the Kv. Agricultural Experiment Station. Of the many theories regarding the cause of anaphylaxis, that offered by Besredka and Steinhardt, (') that the. phe- nomena of anaphylaxis and of anti-anaphylaxis result from conditions similar to the precipiting and absorbing actions which govern the relations of colloids among themselves, is of interest. Von Behring’s (°) claim, that anaphylaxis results from thrombosis of the cerebellar vessels caused by agglutination of the blood platelets, is of further interest. (2) Ann. de I’Inst. Past.; 1907, 21, p. 384 otck (2) Deutsche. Med. Wchnschr. Leipz. u. Berl., 1914, 40, p. 1857 104 THE KENTUCKY ACADEMY OF SCIENCE Kopaczeoski and Vahram (*) maintain that anaphylac- tic shock is a physical phenomenon. They demonstrated that the introduction of suspensions or of colloidal gels into the serum disrupted the colloidal equilibrium, producing flocculation of colloids, obstruction of the capillaries, and death from asphyxia. Among recent investigators there is a distinct tendency to consider colloidal precipitation the cause of anaphylaxis and also, of specific agglutinations. Of the many conditions associated with the colloidal state none is more important than the hydrogen ion con- centration. As Beatty (4) points out, colloids are distinctly influenced by hydrogen and hydroxyl ions, tending to pre- cipitation under their influence, the rule being that ions possessing an electric charge of opposite sign to that of the colloidal particles precipitate such particles. The ions are precipitated with the colloidal particles and, in certain cases, may be removed by washing, which indicates a physical and not a chemical action. Bayless (°) has shown that the intravenous injection of half normal hydrochloric acid, in quantity sufficient— 10.4 cc per kilogram of body weight—to reduce to one-third of its normal value the bicarbonate present, does not alter the H-ion concentration of the blood. Crile (°) has shown that in certain conditions, as nar- cosis, anaesthesia, and shock, the H-ion concentration of the blood may increase with the increased CO. tension. The greatest concentration which Crile observed was pH 6.98. Crile states the normal value as pH 7.67 and Bayless states it as pH 7.4. It occurred to us that it would be of interest to know the H-ion concentration of the blood during or immediately after anaphylactic shock. This we determined in the fol- lowing manner: (83) Compt. rend. d’l Acad. des Sciences, 169, 1919, p. 250 (4) The Method of Enzymic Action, James Beatty, 1917, p. 16 (5) Jour. Physiol., 53, 1919, Nos. 3-4, p. 162 (6) Amer. Jour. Physiol., 38, 1915, P. 225. TWELFTH ANNUAL MEETING 105 Five guinea-pigs, Nos. 29, 31, 33, 34 and 35, average weight of 397 grams, were each given intraperitoneally, 2 cc of a 1 to 1 solution of fresh egg-white in distilled water. Two guinea-pigs, Nos. 32 and 386, average weight of 569 grams, were placed under similar conditions but received no ege-white; they were controls. Four months later, the control pigs, Nos. 32 and 36, to- gether with another normal pig, were anaesthetized with ether, the hair clipped over the cardiac area and this area washed with 98% alcohol. Using a sterile, oiled (paraffin oil), and chilled hypodermic syringe, 4.5 cc of blood was withdrawn from the heart, and immediately transferred to an oiled and chilled centrifuge tube and centrifuged for 10 minutes. The cells were thrown down but the plasma coagu- lated. The coagulum was separated from the cells, cut into small pieces and each piece tested with a drop of either phenol red or cresol red in .02% solution. The resulting colors were compared with those obtained with the standards recommended by Clark and Lubs (*). The H-ion concentra- tion of the coagulum was pH 7.8 in each case. These guinea- pigs recovered. We were unable to obtain blood from the heart during anaphylactic shock; therefore, immediately after death, we exposed and opened the heart, obtaining such blood as it contained. The quantity of blood thus obtained was insuf- ficient for centrifuging. It was, therefore, diluted with 4 volumes of distilled water. Clark and Lubs (°) have shown that colored or cloudy liquids may be diluted in the propor- tion of 2 cc of the liquid to 10 cc of distilled water with but slight change of the H-ion concentration. They determined electrometrically the H-ion concentration of various colored and cloudy liquids: such liquids were then diluted in the above proportions, and the H-ion concentration determined colorimetrically. Of two hundred such determinations the (<)> Jour. -Bact., Vol. 2, Nos. 1, 2, 3, pp. 1, 109, 191. (8) Ibid. 106 THE KENTUCKY ACADEMY OF SCIENCE average variation between the two methods was .11 of one pH value, the maximum being .75 and the minimum being .O1 of one pH value. Four months after the initial injection the test pigs each received, intraperitoneally, 5 cc of a 1 to 1 solution of fresh egg-white in distilled water. With pigs Nos. 29 and 381, marked convulsions occurred in twenty minutes. They were anaesthetized, an attempt to draw blood from the heart failed, the pigs dying within five minutes from the onset of convulsions. After death the heart was quickly exposed and 2.5 ce of liquid blood obtained from it. This blood was quickly diluted 1 in 4 with distilled water and the H-ion con- centration of the dilution determined, using cresol red, .02% solution. The H-ion concentration in each case was pH 6.5. Pigs Nos. 33, 34 and 35 were anaesthetized immediately fol- lowing the second dose of egg-white and blood removed from the heart before the onset of convulsions. The H-ion con- centration of such blood was in each case pH 7.8. Death from anaphylactic shock occurred in each case within twenty minutes after the second dose of egg-white. Immediately after death, blood was obtained from the heart, diluted 1 in 4 with distilled water, and the H-ion concentraton deter- mined as with pigs 29 and 31. The H-ion concentration was, in each case, pH 6.5. Summary. Among recent investigators there is a ten- dency to consider colloidal precipitation the cause of ana- phylaxis. No condition of colloids is more important than the H-ion concentration. The H-ion concentration of the blood of anaesthetized normal guinea-pigs and of guinea- pigs before the onset of anaphylactic convulsions was pH 7.8. The H-ion concentration of the blood of guinea-pigs immediately after death from anaphylactic shock was pH 6.5 which is a marked increase compared with the maxi- mum increase under CO. tension of pH 6.98 as determined by Crile. TWELFTH ANNUAL MEETING 107 (5) The Vegetation of the Barrens. Harrison Garman. What is here presented is merely a suggestion of a point of view, but is based upon a careful study of the flowering plants of Western Kentucky, these brought into comparison with the prairie flora of Illinois with which the writer has been familiar from childhood. It may be thought too late to determine what the vege- tation of the region called the barrens was when the coun- try was settled, but enough may be gathered from early writ- ings to show its general character, and a study of the plants now found growing wild in these parts of the State and their comparison with those of other parts of Kentucky gives, it is believed, a fair conception of the plant life of the great treeless areas as they were when the white man appeared in Kentucky. It has often been asserted, and need hardly be repeated, that the barrens were not regions of sterile soils. The soils were and are in fact of good quality in many places, but ranged widely from poor to rich. The feature of the barrens that most impressed the pioneers was the lack of forest growth. They were not used to it. It was prairie, in fact, and like the settlers of prairie states north of us they were afraid at first to take up farms in this open country and chose land along the watercourses. Coming thru the densely wooded sections of Eastern Kentucky the vast open plains must be accounted for as something un- natural. The most obvious explanation of the general ab- sence of trees was accepted by them, namely, the destruc- tion wrought by forest fires started by the Indians. This explanation has been passed on to our time, and has done service from pioneer days as an explanation of the prairies of the North. But the important fact seems to have been overlooked that forest fires do not result in a typical prairie vegetation such as was present in the barrens and in the prairie region of Illinois. Forest fires in Michigan and other northern states have been of frequent occurrence, but in a short time 108 THE KENTUCKY ACADEMY OF SCIENCE the burnt-over land is occupied again by woody growth simi- lar to that destroyed. Among some of this young growth can now be found charred stumps and logs of the primitive for- ests. Nothing of this sort was observed in the prairies or in the barrens. They had evidently been prairies and nothing else for hundreds of years. When our barrens were first visited by botanists they were described as covered with waving grasses ‘‘so tall as to conceal a man on horseback.”’ In wet places were rushes and sedges, on drier ground a great variety of flowers, goldenrods, asters, shootingstars, adder’s tongues and the rest, low-growing plants in the great- est profusion, with only a few woody species, dwarf willows, blackjack oak and the like, pushing in along the streams from surrounding forests. Many of the woody plants came from the East, from the South and Southeast. These great meadows have now largely disappeared and the places of the tall grasses and other plants have been taken in part by weeds from foreign countries, but the native species are still to be found by the botanist in bits of waste land along railroads and highways and probably few have been completely exterminated. The tall grasses of the early writers were undoubtedly the same as those of the prairie regions north of the Ohio River. The species are still here, but could not stand the close grazing to which they were subjected by the settlers, and have retreated to such shelt- ered places as they can find. The tall bluestem (Andropogon furcatus) was one of the dominant grasses and probably con- stituted much of the tall growth among which cattle grazed, as it did in Illinois. Both bluestem and Indian grass (Sorg- hastrum nutans) actually grew tall enough to conceal a man on horseback in both the prairies of Illinois and the barrens of Kentucky. The original prairie grasses were certainly not Ken- tucky bluegrass. In the barrens this grass does not now thrive very well in most sections after all its tall native competitors have been scattered and greatly reduced in num- bers. As late as 1868 whole sections of land in Central I[lli- TWELFTH ANNUAL MEETING 109 nois had been untouched by the plow and were covered by their original prairie flora, the tall bluestem often domin- ating and affording both pasturage and hay. A little later a fringe of bluegrass mixed with ragweed and other intro- duced plants began to creep in from surrounding cultivated land. Bluegrass seems to have invaded our barrens in simi- lar fashion and tracts of land never tilled can be found, or could within a few years, with the original prairie grasses still dominating. The expression “taller than a man on horseback”’ never applies to our bluegrass, as any one fam- iliar with it knows. The barrens seem to have been merely outlying tracts of prairie of the same nature, the same origin and plants, as the prairies of Illinois. It seems certain that this flora was prepared for by a removal of vegetation over large tracts during glacial and post glacial times and the prairie flora that first appeared was assembled by conditions of moisture and temperature unfavorable at first to the estab- lishment of forests. Attention was first drawn to these considerations by noting the strong resemblance of the present native flora of the barrens to that familiar to the writer in childhood on the prairies of Central Illinois. Many of the dominant prairie plants of the regions north of the Ohio River are still to be found in the barrens. Tall bluestem, a dominant prairie grass, is there. The small bluestem (Andropogon scoparius), its associate, is present. Indian grass (NSorghas- trum nutans), one of the very tall species, has not entirely dis- appeared. It may be said that a large proportion of the typi- cal prairie plants of Illinois still exist in the barren regions of Kentucky. With the grasses named, occur the purple and white prairie clovers (Petalostemum), the button snakeroot (Eryngium — yuccifolium), Indian hemp, two blazing-stars (Liatris), the cup plant, compass plant (Nilphiwm laciniatum), and the purple coneflowers. Such assemblages of species do not appear after fires in other regions of the State. They did not appear in either 1106 THE KENTUCKY ACADEMY OF SCIENCE the Eastern or Western Coal Fields. The plants did not assemble here in a year or two. It required hundreds of years to establish a turf that resisted the penetration of forests except where the soil was disturbed along streams. It seems probable that if the white man had not appeared, these open tracts of meadow would exist today. The pioneers were witnessing, not, as they supposed, the results of recent disturbances of plant conditions due to the Indians, but the much more profound effects of glacial conditions* during which plants were destroyed over large areas, leaving swamps and boggy tracts at first, these as the temperature rose and the waters subsided affording an op- portunity for growths of rushes, in turn to give place, as the land became drier, to sedges, the climax vegetation of tall grasses and Compositae appearing finally and covering much of the affected territory when the pioneers arrived. Cheracteristic Plants of the Barrens, also Characteristic of the Illinois Prairies: Scouring-rush (EKquisetum hyemale) Cattail (Typha latifolia) Little bluestem (Andropogon scoparius) Tall bluestem (Andropogon furcatus) Indian grass (Sorghastrum nutans) Switchgrass (Panicum virgatum) Panicum scribnerianum Mesquite grass (Bouteloua curtipendula) Turk’s cap lily (Lilium superbum) Partridge pea (Cassia chamaecrista) Wild sensitive plant (C. nictitans) Purple prairie-clover (Petalostemum purpureum) White prairie-clover (Petalostemum candidum) Button snakeroot (Eryngium yuccifolium) Indian hemp (Apocynum androsaemifolium ) *The writer must not be assumed to hold that the ice cap ex- tended into Western Kentucky. It is well Known that it reached but little south of the Ohio River and only in Eastern Kentucky. The in- fluences referred to are those of low temperature and of great quan- tities of cold water coming from melting ice at the North. TWELFTH ANNUAL MEETING alata Indian hemp (Apocynum cannabinum) Butterfly-weed (Asclepias tuberosa) Green milkweed (Acerates viridiflora) Green milkweed (Acerates floridana) Puccoon (Lithospermum canescens) Wild bergamot (Monarda fistulosa) Venus’s looking-glass (Specularia perfoliata) Cardinal flower (Lobelia cardinalis) Blazing-star (Liatris squarrosa) Blazing-star (Liatris spicata) Goldenrod (Solidago canadensis) Goldenrod (Solidago altissima) Rosin-plant (Silphium terebinthinaceum) Rosin-plant (8. trifoliatum ) Cup-plant (S. perfoliatum) Compass-plant (S. laciniatum) Silphium integrifolium Purple conefiower (Brauneria purpurea) Coneflower (Brauneria angustifolia) Pale Indian plantain (Cacalia atriplicifolia) (6) Preliminary Inoculation Experiments with Fusa- rium Species on Seedlings Growing in Nutrient Agar. W. D. Valleau and E. M. Johnson. The impression appears to be quite prevalent that the Fusaria as a group are somewhat specific in their host pre- ferences. This is especially true of the wilt-producing species which it would seem from a review of the literature are only capable of attacking a single species or at best only species within a genus. Wollenweber (‘) in describing F. tracheiphitum Smith and F. vasinfectum Atk., states that they cause the wilt disease of Vigna, and Gossypium her- baceum and G. barbadenese, respectively, and are “Sapro- phytic on various hosts”. He states also that “the parasite from one host, as a rule, has not been found on the living organs of another host.”’ Taubenhaus, in his “Diseases of Truck Crops (p. 47) ex- presses the same idea when he states that “The Fusarium (1) Phytopath. 38; 24-50, 1913 112 THE KENTUCKY ACADEMY OF SCIENCE of the sweet-potato wilt cannot, as far as we know, attack potatoes, tomatoes, or any other host. This is similarly true for the Fusarium which produces a wilt on tomatoes, etc.” Bolly (°) states that; “Though living normally as a decay form (saprophyte) this low type of fungus (F. lini) can also invade the living tissues of its host. As it can live and increase upon decaying matter found in soils, especially upon old roots and stems of flax, it can readily thrive there for a long period.” And further; “It can live from year to year upon the humus of the soil, hence the soil is soon ruined for flax.” This implies that the organism is quite specific for the flax crop and does not live on other living plants. Tisdale (*) in studying the nature of wilt-resistance in flax found ‘that Fusarium conglutinans would penetrate the root hairs of flax seedlings. Likewise, F’. lini was evidently able to penetrate cabbage seedlings as they were killed by it in tube cultures.” He also found “that F’. lini can pene- trate the young seedlings of the resistant strain of flax as readily as it can penetrate the seedlings of the susceptible strain under those conditions”’. Pratt (*) has stated that F. trichothecidides, F. radicicola and Rhizoctonia solani, organisms which are commonly con- sidered as potato pathogens, are found in the virgin soils of southern Idaho. Bisby (°) made cross inoculations with several species of Fusaria and as a result of these studies and other obser- vations states that “Rots of carrots and other vegetables due to various Fusaria are quite common, particularly in stor- age and following wounds.” But “despite their semi-para- sitic nature many Fusaria, such as F. orysporum, exhibit (2) North Dakota Agr. Expt. Sta. Bul. 50: 27-58, 1901 (3) Jour. Agr. Res. 11: 573-606, 1917 @) Journ, Agr “Res. 132 (3-005. FOS ) Minnesota Agr. Expt. Sta. Bul. 181, 1919. TWELFTH ANNUAL MEETING 113 what amounts to a considerable specificity of parasitism to certain crops’. The impression to be gained from these statements and others of a like nature which can be found in the extensive Fusarium literature is that the wilt-producing Fusaria are quite specific in their host preferences and live from one crop of the host to the next, even tho the interval be one of many years, aS saprophytes in the soil. That this is prob- ably not the only method of survival in the soil is indicated by the inoculation experiments to be reported in this paper. Milier (°) working in this laboratory, made numerous isolations from tobacco roots affected with the so-called brown root-rot both from fields in which the root-rot was severe enough to cause extensive injury to the plants, and from fields in which the injury was comparatively slight as judged by the development of the plant. The Fusaria obtained have not yet been completely iden- tified, but a comparative study of them indicates that quite an extensive list of species are concerned. Miller made inoculation tests, with 7 of the cultures which he isolated, on young tobacco seedlings growing in tubes on nutrient agar and found that all of them killed the plants in a period of from 3 to 10 days. Since then we have carried on extensive inoculation experiments in test tubes both with seedlings just producing the first true leaf and with older plants having several true leaves. The results of these tests may be summed up by saying that nearly all of the Fusaria isolated from tobacco roots cause injury to to- bacco seedlings under the conditions of the experiment. The results of these tests indicated so clearly that tobacco was susceptible to many species of the unidentified root Fusaria that a number of the better known species were obtained thru the courtesy of Dr. E. C. Stakman, University {*) Miller, Paul W., Brown root-rot of tobacco. University of Ken- tucky thesis, 1924. 114 THH KENTUCKY ACADEMY OF SCIENCE Farm, St. Paul. These have been tested, in comparison with some of the identified Fusaria isolated from tobacco, on seedlings of various kinds. The identification of our cul- tures of tobacco and other Fusaria used in these tests have been made, in some cases tentatively, by Dr. C. D. Sherba- koff. They are as follows: F. acuminatum Ell. et Ev.? from alsike seed; F. succisae (Schroet.) Sacc. from tobacco seed; F. solani (Mart.) App. et Wr. and F. oxysporum like, but differing in certain characters, from red clover roots; F. argillaceum (Fr.) Sare.; FF. angustum Sherb.; F. aurantiacum (Lk.) Sacco.; F. bulbigenum Cooke et Massie; F. falcatum App. et Wr., F’. lycopersici (Sacc.) Wr.?, F. orthoceras App. et Wr.?, F. solani cyanum Sherb.?, and Gibberella saubinetii? from tobacco roots. The following were identified by one of the writers, G. saubinetii from a wheat seed, fF’. moniliforme from a corn seed and F. moniliforme from clover roots. The named species of Fusaria obtained from Dr. Stak- man are as follows: F’. conglutinans, F. conglutinans calistaphani (Michigan Agricultural College No. 58), F. discolor sulphureum (North Dakota 2007), F. lycopersici (C. D. Sherbakoff), F. lycopersici (M. A. C. 158), F. lycopersici (S. H. Essary), F. lycopersici (Edgerton), F. martii? (F. R. Jones), F. lint (No. 54), F. lini (H. O. B.), Gibberelia saubinetii (HE. C. $.), and G. scubinetii (MacInnes thesis), F. batatatis (M. A: C. 60), F. batatatis (Massachusetts), F. orysporum (M. A. C. 52), F. oxry- sporum (G. R. B.), F. oxysporum resupinatum (M. A. C. 52), F. oxysporum asclerotium QCM. A. C. 56), F. hyperoxysporum (M. A. C. 57), F. orthoceras triseptatum and F. radicicola (M. A. C. 84). Gibberella scubinetii (Wis. 259) was received thru the courtesy of Dr. James Dixon, University of Wisconsin. The inoculations reported in this paper were all con- ducted on seedlings growing in test tubes of nutrient agar. The tubes containing the young plants were kept in the light near a window in a room kept uncomfortably warm (75+°F). Inoculations were made by transferring a small bit of mycelium from a vigorously growing culture to the TWELFTH ANNUAL MEETING 115 surface of the agar and usually near the plant. It was then necessary for the fungus to spread over the agar and pene- trate the uninjured seedling. This development usually re- quired at least 2 days so that this time might be subtracted from the time required to kill the plant.. The seed were treated in various ways to rid them of seed-borne organisms or at least to prevent the development of these organisms in the tubes. The details of the various inoculation tests are too ex- tensive to be given at this time but a summary of the re- sults obtained and their possible significance will be given. In all, 25 distinct named species or varieties of Fusaria have been used in these tests, together with a large num- ber of isolations from the roots of various crops as tobacco, corn, clover, etc., including isolations from several weeds. These root organisms have not been completely identified but the majority fall into the section Elegans of the genus Fusarium, a section which contains the most important wilt- producing Fusaria. The remainder belong, with a few ex- ceptions, to the section, Martiella, which is supposed to con- tain but very few pathogenic forms. The named species were tested on seedling plants of corn, soybeans, wheat, radish, onion, tobacco, clover, alfalfa and cabbage. In all more than 1000 inoculations were found to be capable of killing at least some of the species of plants inoculated in a period of five to ten days from the date of inoculation. The majority of them were capable of partially or completely destroying the roots of all of the plants, with the exception of corn, within a period of about 12 days. Corn was found to be the most resistant, of the plants tried, to injury by these organisms. Fifteen of the cultures used had caused no injury to corn seedlings after 14 days, while 22 cultures had rotted a portion of the roots, in some cases killing the seedling. Tests with organisms other than Fusaria, some of which are generally considered to be somewhat pathogenic, as 116 THE KENTUCKY ACADEMY OF SCIENCE Alternaria sp., and others generally not so considered, as the common penicillia, have shown that these organisms usually cause less injury than the least pathogenic of the Fusaria tested. Certain of the Fusaria as Gibberella scaubinetiii, F. suc- cisae, a wilt-producing strain of F. lycopersici (S. H. Essary) and others proved to be very injurious to all of the plants tested. A few cultures, as Ff’. hyperoxrysporum, F'. lint (A. O. B.), F. faleatun and F. acuminatum caused very little injury to nearly all of the plants against which they were used. Extensive tests of the unidentified fusaria from roots of various crop plants show that nearly without exception they are capable of causing the death of seedlings of clover, alfalfa and tobacco and of penetrating either slightly or ex- tensively the roots of corn. An interesting feature of this part of the work has been the results obtained with cultures belonging to the section Martiella, usually considered non- pathogenic. The cultures of the Martiella section, most of which were probably F’. solani, appeared to be just as injurious to clover, alfalfa and tobacco seedlings as most of the cul- tures of the Elegans section. They were, on an average, only slightly less injurious to corn seedlings than the most in- jurious of the cultures of the Elegans section, and decidedly more injurious than many others of this section, particulariy those tentatively identified as F. «ngustum. It appears from these inoculation experiments that the species of Fusaria commonly isolated from the rotting roots of plants, the common wheat scab organism, and several of the wilt-producing species of Fusaria are capable, to a greater or less degree, of injuring and penetrating the ten- der roots of seedlings of several common crop plants. The wilt producing species appear to be particularly injurious as evidenced by these tests. When we consider the severe competition going on between micro-organisms in the soil, and the fact that under certain conditions these fusaria are capable of penetrating the injured roots of several crop TWELFTH ANNUAL MEETING 11, plants, the possibility is suggested that they are not soil saprophytes in the true sense of the word, but rather weak parasites on a large number of plants, main- taining themselves from year to year partly by rotting rootlets and partly by remaining in these decayed tissues the remainder of the year. In the case of the wilt-producing Fusaria, which remain in cultivated soils for many years in the absence of their specific host, the conclusive determina- tion of this point is of economic as well as scientific interest. (7) A Kentucky Thorobred. G. D. Smith. The paper described a fine thorobred racehorse that was, when young, a little difficult to start in a race and grew more so each year until finally he became useless as a racer. (8) Relation of Some Constituents of Tobaceo to Grade. O. M. Shedd, Kentucky Experiment Station. (Synopsis) This investigation is a comparative study of the total content of nitrogen, nicotine, nitrate nitrogen, crude ash, silicon, phosphorus, potassium, calcium and magnesium found by chemical analysis in the different grades of tobacco represented by 145 samples of burley and 109 samples of dark tobacco grown in Kentucky in 1920 and 1921. The mineral constituents were determined in composite sampies but the other determinations were made in the individual samples. The burley crop of 1920 was abnormal, being infected to an unusual degree with leaf-spot diseases. The dark tobacco of that year employed in this investigation, was less diseased than the burley. Both burley and dark tobacco samples taken from the 1921 crops were more nearly nor- mal, as they were freer of disease. This affords a compari- son of abnormal versus normal leaf, as well as variations in different years. The “good” division of any grade usually contained a larger percentage of nicotine than the “common”. The nit- 118 THE KENTUCKY ACADEMY OF SCIENCE rogen and nitrate were more variable but in the majority of instances, the “‘good” carried larger percentages of these constituents than the “common”. Of the burley grades, the fillers usually contained the largest percentages of nitrogen and nicotine and the smokers, the smallest. In the dark tobacco the leaf usually had the largest percentage of nitrogen, nicotine and nitrate, and the trash the smallest. The burley tobacco of 1921 contained larger percentages of all constituents determined, except calcium, than that of 1920. The dark tobacco of 1921 also carried larger percent- ages of all except calcium and phosphorus, than that of 1920. The proportions of phosphorus and magnesium, altho dif- ferent in the two kinds of tobacco, showed the least varia- tion in the same crop, in the two years. The burley tobacco of each year contained larger per- centages of nitrogen and nitrate but a smaller percentage of nicotine than the dark tobacco. The combined burley samples showed 4.5 per cent more total nitrogen and 78.8 per cent more nitrate nitrogen, but 40.9 per cent less nico- tine than the dark. The ranges for the nitrogen constituents determined in the dry burley were 1.96 to 6.05 per cent total nitrogen, 0.29 to 6.18 per cent nicotine, and 0.009 to 1.37 per cent nit- rate nitrogen. For the dry dark tobacco they were 2.07 to 5.75 per cent total nitrogen, 2.12 to 7.83 per cent nicotine, and 0.002 to .73 per cent nitrate nitrogen. The proportions of the ash constituents, other than phosphorus and magnesium, differed considerably in the “good” and “common” grades. The ‘“‘good’’, apparently, had the most potassium. The averages obtained for burley tobacco apparently show that the wrappers had the smallest percentages of silicon and calcium but the largest potassium; the fillers, the smallest percentages of ash and potassium, and the TWELFTH ANNUAL MEETING ay) smokers the largest percentages of ash, silicon and calcium. The averages for dark tobacco show that the leaf had the smallest percentages of ash and silicon but the largest of potassium and calcium, while the trash had the largest per- centages of ash and silicon but the smallest of potassium and calcium. The burley tobacco of each year contained smaller per- centages of ash, silicon and magnesium, but larger per- centages of phosphorus, potassium and calcium than the dark tobacco of the corresponding year. The averages of the analyses of burley tobacco representing the two years show 1.7 per cent less ash, 67.7 per cent less silicon and 36.9 per cent less magnesium, but 81.0 per cent more phosphorus, 51.7 per cent more potassium and 37.8 per cent more cal- cium than the corresponding averages for the dark tobacco. Burley leaf badly infected with leaf-spot disease con- tained a considerably smaller percentage of nicotine but a larger percentage of nitrogen than that which was less diseased. This indicates that such tobacco either had part of its nitrogen in combinations which were detrimental or the deficiency of nicotine vitiated its quality. (For the de- tailed report see Ky. Experiment Station Bulletin No. 258.) (9) seine \ / P 1 0 mit IPQNSTHNU amTIVON ‘ \ =~ * ; \ 7 \ . ‘ e SNIMdDOH a eSNIA a =e rf & Wa - LAC NIONIf~ LI8D.. eel wy NVaDef) Osatseam/” =e 3 y 6 V/ Loe s=aay NOINN yf NIGYvH / “> seaiava ae Bie bea, NOSUION. AN wrRaW aa s AN N aes Gs Cans. fcsenomey oy faarvee ef, veo aris "PT boug abouloup ul SIL D= 44°65 Us buivedo pasynbey : oe me LO/NUIIOS eee ‘O/OUbd Qf $£09/OL U1 2 JO SEN/OA 2601810 bu/MoYys AWORQLNGAYW JO dvW 222 THE KENTUCKY ACADEMY OF SCIENCE This condition gives at once a reasonable and satisfactory explanation for the increase from east to west of values of “C” in Talbot’s Formula. Having thus determined the causes for the sectional variations in the average values of “C’’ which had been com- puted, the map on which these had been plotted was com- pleted by drawing lines, much like contour lines, assigning values to all sections of the State. These lines were made to conform to the computed values, which had been deter- mined from actual field observations, and the Isopluvial Charts and the geological map were used as guides in pro- jecting them across sections where no definite field infor- mation had been obtained. The engineer in the field can, from this map, determine the average value required for “C” in Talbot’s Formula in the section in which he is working. In applying this to a particular problem, he must consider whether the topo- graphical conditions in the drainage area being examined might be considered normal for the general section and if not, he must use his judgment in making a corresponding adjustment. Factors, such as storm rainfall intensity, which he cannot determine from an examination of the ground, are taken care of in the average, sectional value, and with experience he should be able to make the adjustment re- quired by variations in topography, etc. The investigations were considerably handicapped be- cause of the fact that only about half of the State has been topographically mapped and drainage areas could not be accurately determined in many sections without ex- pensive surveys. It is hoped that further studies will per- mit the construction of a more accurate map and will also indicate more clearly the adjustment which should be made on account of variations in topography, vegetation and stream conditions. THIRTEENTH ANNUAL MEETING bo be wo (18) .A Newly Discovered Prehistoric Fort in Ken- tucky.* Wilbur Greeley Burroughs, Berea College. A prehistoric stronghold which white men have never before known existed, was discovered by the author, Novem- ber 9, 1925. This fort is situated 31-2 miles east of Berea, Madison County, Kentucky, and one-half mile northeast of the north end of Indian Fort Mountain previously explored and surveyed by the author. The newly discovered fort in- cludes the entire top of a knob which is Separate from all other knobs except for a narrow ridge at the base of the cliffs on the west end of the fort. This ridge extends to the cliffs on a knob to the west of the fort. The lower portion of the knob on which is the prehistoric fort, consists of a steep shale and talus slope which rises rapidly four hundred feet vertically above the adjacent valleys. Above this slope, cliffs of Mammoth Cave and Gasper limestone, except at one point, completely surround the upper parts of the knob. The limestone is broken in a few places by joint planes two to four feet wide up whose extremely steep floors an active climber can reach the top of the cliff. Two of these joint planes occur at the west end of the fort where the limestone cliff is fifty feet high. The east end also has a similar crevice. Along the north side the limestone has been worn away for a few yards but all of these points can be defended easily. A narrow terrace occurs at the east and west ends of the knob and at irregular intervals along the sides where the limestone is capped by Pottsville conglomerate, in places forty feet high. Joint planes occasionally break this con- glomerate cliff, but access to the crest could easily be pre- vented by determined peopie armed with stone weapons. At certain points the conglomerate and limestone cliffs unite and fall 200 feet straight down where the talus does not rise high along the base of the limestone. On the south side a stream has worn away the cliffs and formed a ravine 224 THE KENTUCKY ACADEMY OF SCIENCE which is the only readily accessible entrance to the top of the knob and fort. Across this ravine the prehistoric people constructed a stone barricade 465 feet long which extends from the top of the limestone cliff on the east to the top of that on the west. The barricade is in the form of a V with the apex upstream. At present this rampart is in places five feet two inches vertically from the top to the bottom of the front side, and at this point seven feet across. The V shape of the barricade permitted the defenders to hurl missiles upon an attacking force from three sides. This barricade differs in shape from those on the large prehistoric fort of Indian Fort Mountain where barricades crossing ravines of equal width are crescent-shaped. The top of the newly discovered fort is gently rolling and covers about eighteen acres. , A few inches back from the edge of the conglomerate on the north central part of the fort is a roughly rectangular basin which has been hollowed out of the conglomerate. The rock here is exactly the same as in other exposed sur- faces of this knob. The basin is eight feet by five feet eight inches by five inches deep. The long axis extends about northwest--southeast. The basin contains water. A theory for the origin of this basin which the author suggests is that since there is no spring within the walls of the fort, as far as known, the prehistoric people who held the fort may have drawn water up the side of the cliff and emptied it into the basin which they had hollowed in the rock. Skins could have been used to hold the water and grapevines or other material used for rope. On another promontory to the east is a smaller basin in the conglomerate which ap- pears to have been just started when work was abandoned. This second basin is also rectangular and located near the edge of the precipice. .These points are the best places on the fort for water to be drawn up the cliff and poured into the basins. Also, being exposed to the sky, they easily catch rain-water. Because of these basins, this knob will be called THIRTEENTH ANNUAL MEETING 225; “Basin Mountain’, and the prehistoric stronghold, “Basin Mountain Fort’’. Along the east and northeast sides of the fort especially where the conglomerate comes in contact with the under- lying limestones, many of the joint planes have been en- larged by solution into long, narrow caves. Fifteen to twenty feet from the entrance to these caves, they usually become larger where the limestone floors have been dis- solved away. In several caves are pits about 150 feet deep in the limestone. In two caves thus far explored are num- erous pieces of charcoal and charred branches and sticks. Some of these pieces are far back toward the interior of the caves where water could not have washed them. In- deed it is doubtful whether water could have washed any of the pieces of charcoal from the entrance to their present positions as many large boulders of conglomerate cover the floor of the cave and block the passage so that these rocks have to be climbed over to penetrate the cavern. It would appear as tho these pieces of charcoal had been taken into the caves by the prehistoric inhabitants while the charcoal was glowing. The warm charcoal would have given heat to those clustering around without making any disagreeable smoke. The ventilation is excellent in these caves and the temperature is comfortable even without fire when snow is falling out-of-doors. It would seem improb- able that the charcoal and charred branches were carried to their present positions by white hunters, as in order to reach these caves on the northeast side of the knob, one has to descend a 20 to 30 foot cliff and move carefully along a narrow terrace below which the limestone falls away for about 100 feet. The most logical explanation for this char- coal is that it was taken into the caves by the prehistoric defenders of the fort, who lived in the caves during cold and rainy weather. One cave has a large, oval-shaped mound of earth near the entrance. A boulder of conglomerate lies across the en- 226 THE KENTUCKY ACADEMY OF SCIENCE trance, placed there evidently by the prehistoric people. It prevented the earth of the mound from being washed out of the cave. In the mound are scattered patches of the whitish substance similar to that found in the graves of the rock- houses of the Indian Fort Mountain stronghold. Pieces of charcoal are mixed thru the earth of the mound. It is a place of burial. At the west end of the knob, below the limestone cliff, a perpendicular rock wall about 220 feet long and two to four feet high extends from near the south side of the joint plane in the limestone partially across the narrow ridge of land that stretches from the fort to the knob to the west. It is not a fence made by white men as it does not extend the entire length of the ridge and could not keep cattle con- fined to any spot on the slopes. It is probably part of the defences of “‘Basin Mountain Fort’’. The fort has not as yet been thoroly explored and further investigation may reveal new facts about these prehistoric people. (19) On the Origin of Fibrous Serpentine. L. C. Robin- son, U. of Ky. (By title) (20) Subsurface Conditions in Northeastern Kentucky as Shown by Well Reeords. E. S. Perry. (By title) (21) Geology of the Oil Shales of the Eastern United States. W. R. Jillson, State Geologist, Frankfort, Kentucky. (Abstract) It has been estimated that at the present rate of pro- duction and consumption—714,000,000 barrels in 1924—the known petroleum reserves of the United States will be ex- hausted within fifteen to twenty years. The oil shales of the United States broadly distributed and vast in quantity are suggested as the ultimate source of substitute artificially produced petroleums. The Green River (Eocene) oil shales THIRTEENTH ANNUAL MEETING 227 of the West, rich and extensive, having been abundantly dis- cussed, this paper concerns itself only with the high-grade bituminous shales of the Eastern United States. These are divided into three groups, ascending stratigraphically: (1) Chattanooga, early Upper Devonian; (2) Sunbury, Lower Mississippian, and (38) various oil shales and cannel-shales of the Pennsylvanian. The first group—Chattanooga (New Albany—Ohio) black shale of early Upper Devonian age is rich, thick and extensive. The type locality described is Southern Indiana and Ohio, Central Kentucky and Tennessee, and Northern Alabama, but low-grade correlatives extend into Missouri, Illinois, Iowa, Michigan, Ontario, New York, Pennsylvania, West Virginia and Virginia. This shale is estimated to pre- sent at the surface alone ready for operation when economic conditions became favorable 275,000,000 thousands of tons of easily workable deposits equivalent to 60,000,000 thous- ands of barrels of oil, a volume sufficient to supply the United States at the present rate of consumption for upwards of one hundred years or more. The Sunbury (Mississippian) and the various Pennsylvanian high-grade bituminous shales are regarded as oil shale reserves of secondary importance. (The complete paper was read at the Fourteenth Interna- tional Geological Congress, Madrid, Spain, May, 1926). (22) Some Electronic Structural Formulas. V. F. Payne, Transylvania College. (Abstract) Four structural formulas of hydrogen peroxide are con- sidered :1) H-O-O-H,;; (2) Kingzett’s formula, #-O =O; (3) ae Bruhl’s formula, (4) H-O =O-H; and Rins’ formula O:::O . He It is pointed out that by dropping one bond between the oxy- 228 THE KENTUCKY ACADEMY OF SCIENCE gen atoms in Kingzett’s formula it becomes a tautomer of formula (1). The corresponding electronic formulas are (1) H H-O..0-u and HOO. (23) A Simple Method for the Determination of In- ductanece. R. B. Scott, U. of Ky. Object: To make an absolute determination of self- inductance. Theory: The usual method of measuring self-inductance by means of the Wheatstone’s bridge (Rayleigh’s method) is somewhat tedious because the bridge must be adjusted each time a different inductance is used. However, in meas- uring mutual inductance the method is very simple; the secondary circuit is connected directly to a ballistic galvano- meter and the inductance is measured by calculating the quantity of charge, Q, from the galvanometer throw when the current in the primary is started or stopped. This sim- plicity suggests the possibility of a similar method for meas- uring self-inductance. The chief difficulty of the measure- ments lies in the fact that in measuring self-inductance the battery circuit and galvanometer circuit cannot be sep- arated so there is danger of current from the battery pass- ing thru the galvanometer. This difficulty is overcome in the circuit shown in figure 1. The current from the bat- tery flows thru the inductance L and the resistance R,, The switch S is especially constructed so that the switch arm leaves the two contacts as nearly simultaneously as possible, thus leaving the galvanometer in circuit with the inductance and resistance. When the switch is opened, the self-inductance of L will cause a current to flow around the circuit L — R; — Rs — G — L, the battery being disconnected. Calling the total resistance of the circuit R, the current thru the inductance as the switch is opened ip, and the inductance L, we may THIRTEENTH ANNUAL MEETING 229 Fig. 1. Wiring diagram for inductance experiment. make a determination of the inductance by measuring the quantity of electricity which flows thru the galvanometer. When a circuit containing an inductance and carrying a current is suddenly opened the E. M. F. induced by the inductance is —L di/dt. but E — iR then —L di/dt — Ri or —Ldi — R idt Integrating both sides of this equation between the proper limits we obtain Li = RQ 230 THE KENTUCKY ACADEMY OF SCIENCE Where Q the quantity of electricity passing thru the galvanometer then L — RQ/ig Q is a function of K, the galvanometer constant, t the period, d the deflection and r the damping factor of the cir- cuit and galvanometer used or Ktdr*% Cee Sszice: RKC TZ d 1 ee 3.1416 i If we keep the resistance of the galvanometer circuit constant, then the first term of the expression is a constant since the damping factor and period are constant for a given total external resistance of the galvanometer. d then == Cl. i This fact makes the method very easy and efficient for we may insert one inductance after another and measure them by reading the current thru the ammeter, which is the current thru the inductance, and noting the deflection. Apparatus and Procedure: The apparatus used in the ex- periment consisted of the special switch, a ballistic galvano- meter, and suitable noninductive resistance; also a double pole, double throw switch for reversing the galvanometer connection. The problem of breaking the two circuits simultaneously was solved by having one element of the switch, as shown in Figure 2, in sliding contact with two parallel brass rods, and connected to a spring which, when released, would cause the element to slide along the brass rods until the ends were reached and then break both circuits. The fact that the sliding element reaches a high velocity before the contacts are broken tends to reduce the time interval between the THIRTEENTH ANNUAL MEETING 231 Fig. 2. Special switch for the experiment. breaking of the contacts until it does not affect the readings, as shown by repeated experiments. The method of measuring the inductance is rather straightforward. The switch must be adjusted, with the in- ductance replaced by a non-inductive resistance, until break- ing the circuits does not deflect the galvanometer. This shows that the contacts are breaking simultaneously. Since there is some resistance in the sliding contacts there will probably be a potential drop across the galvanometer caus- ing a constant deflection. This may be eliminated by ad- justing the resistance, R;, that is in parallel with the in- ductance. An easier and just as accurate method, however, is to leave the galvanometer switch open until ready to take a reading, then close the galvanometer switch and release the inductance switch in rapid succession. Of course if the constant deflection is too great some error will be intro- duced unless the resistance is adjusted. For measuring fairly large inductances where the inductance of the am- meter would be negligible it may be placed directly in the inductance circuit thus enabling one to read the current iy directly. The range of deflection obtained in measuring the va- rious inductances was from 1 to 6 centimeters. For this 232 THE KENTUCKY ACADEMY OF SCIENCE range the constant of the galvanometer was found to be 7.49 X 10-° amps per radion. The half period of the galvanometer was found to be 3.32 seconds. The damping factor of the galvanometer for this cir- cuit was r2 — 1.098. The total resistance in the galvanometer circuit was 40507. A standard inductance in the form of a variometer was first measured. This was a Leeds and Northrup instrument and had a scale calibrated in millihenrys. The inductance was measured for different settings and the results com- pared with the scale readings. Other inductances measured were, a coil of wire con- sisting of 3000 turns of number 22 B & § gage, a large coil with an iron core, and a honeycomb coil such as is used in radio circuits. Deflection Inductance R R I R B Std. .035 18.16 9.08 aT ules 3.08 Std. .030 18.16 9.08 B07 25 2.5 Std. .020 18.16 9.08 eA B bel 4, Std. .005 18.16 9.08 eOUM 42 4 3000T No. 22 9.08 0388 Pal 2.65 Iron Core 9.08 401 175 12583 H.C. 9.08 .056 1.92 1.82 Rk tre d = — xX ——— 3.1416 i L 40507 x 7:49 x 102 x 3.32 x 1098 x a i 3.1416 16; 852 x d i Substituting the values of d and i in this we obtain the values of L. THIRTEENTH ANNUAL MEETING 233 Inductance Value To Measured Obtained Diff Std. .035 .0357 + 2.0% Std. .030 .0298 — 7% Std. .020 .0195 — 2.5% Std. .005 .0047 — 6. % 3000T No. 22 247 Iron Core 0157 HC oil a Le? Conclusion: Since the error was much greater for small inductance, and correspondingly small deflections, we may conclude that the principal source of error is in reading the deflections, for larger deflections can be read more accur- ately. For inductances greater than 20 millihenrys the er- ror was less than 2%, with an increase of accuracy for higher values of inductance. The accuracy for large in- ductances is limited only by observational error in reading the deflections and in determining the constants of the gal- vanometer. (24) The Principles of Eugenies. Abstract of address by Harry H. Laughlin, Carnegie Institution of Washington. Eugenics has been practiced for hundreds of human generations. The proof is found in the fact that man, dur- ing the many thousands of years since the origin of the human species, has greatly differentiated into races, each highly adapted to specific conditions. For the most part, this successful practice was not consciously directed toward race improvement, but was the natural and eugenically un- conscious accompaniment of the struggle for self-preserva- tion. This struggle prompted selective mating and selective elimination. Early human culture had not advanced far before it was observed by the leaders of clans and states that high fecundity on the part of the most valuable members of the social unit constituted the best assurance of future prosperitv. As civilization advanced and the analytical 234 THE KENTUCKY ACADEMY OF SCIENCE sciences took rise, man began to reflect more upon the es- sential principles which govern his own racial and family fortunes. He was thus enabled to foresee a possible science of eugenics. The materials which he used for analysis were the actual records of past behavior of mankind in relation, particularly, to the forces which govern differential migra- tion, mate selection and differential fecundity. A more ac- curate understanding of the rules of heredity of human phy- sical, mental and temperamental qualities made the final major contribution to the essential ‘“‘tools,’ so that finally and but recently the science of eugenics was successfully organized. Guided by science, the practice of eugenics can now take on a more conscious purpose. Without destroy- ing the finest customs and the most cherished family rela- tionships, it is within the power of the several families and races of man greatly to improve the hereditary physical, mental and temperamental endowments of their successive generations. The science of eugenics did not spring forth full grown as an invention without ancestry, but rather it is the logi- cal development of work in many allied sciences, such as biology, geology, anthropology, psychology, history, politics, geography, archaeology, statistics, psychiatry and theology. From these and from many other sources certain facts and principles have been taken and constructed into an harmon- ious whole. Galton did not call eugenics either a science or an art; he called it simply “the study of the agencies un- der social control which may improve or impair the racial qualities of future generations either physically or men- tally”. It is clear from this definition that eugenics in Gal- ton’s conception would develop into a science and also in- to a purposefully applied art. In genetics, as applied to plant and animal breeding, it must be acknowledged that the science arose long after the art of improvement of plants and animals under domestication had made tremendous headway. The rise of the science of genetics is logically THIRTEENTH ANNUAL MEETING 235 expected to reflect back to the applied art new tools for greatly improved efficiency. If, in eugenics, we tried to list categorically a number of basic principles, they might well be stated as follows: 1. Man is an animal and consequently, in reference to hereditary traits, is susceptible to the same laws of natural inheritance which govern other animals. 2. The end result, or development stage, of a human quality is the resultant of the interaction of hereditary ele- ments with training and environment. 3. Environment and training being constant, the char- acter of an individual, family or race depends primarily up- on inborn endowment. 4. The hereditary endowment being constant, the re- sulting character of an individual, family or race depends upon training and environment. 5. A gene, which is the hereditary foundation of a trait or quality, is not affected by association in different com- binations nor by training nor environment. 6. Within the limits of hereditary constitution of the population of one generation, the constitutional character of the next generation depends upon differential migration, differential mate selection and differential fecundity. 7. Mate selection depends primarily upon propinquity and compatibility. 8. Within the same race and social setting, and ex- cepting within the extremes of poverty and luxury, differen- tial fecundity and survival depend largely upon economic factors. The so-called high levels tend toward lower fecun- dity and more conservative survival rates. 9. Human migration is a phase of the struggle for ex- istence among families and among races. Relative over- population and economic stress tend to produce migration to regions less densely populated or in which the struggle for existence is less severe. 236 THE KENTUCKY ACADEMY OF SCIENCE 10. Knowledge of the principles of eugenics makes possible the more purposeful control of the hereditary en- dowments of future families and races. It is clear that a family or a nation must maintain high ideals concerning hereditary constitution, if it desires to raise or even to maintain its present inborn standards in future generations. The economic, the social, the educa- tional and the religious world must work so to govern cus- tom and so to elucidate the facts of eugenics, that more fit mate selections will be made, and that those who are best endowed by nature, so far as the standards of the family and the race are concerned, will have high fecundity, while those less well endowed in hereditary qualities must have the lowest fecundity. Finally, the state must see to it that the very lowest in inborn qualities must not be permitted to reproduce at all. Research and education must lead the way; legislation can function only in bringing up the rear. When coldly considered, we know that there are assets which control falling in love, such as social position, wealth, talent, education, gracious behavior, charming personal qual- ities and comely features. When hereditary endowment be- comes one of these major factors and possibly the overpow- ering factor in mate selection, altho the person who falls in love may not clearly recognize the fact, then family and consequently national eugenics will be established on a sound practical basis. The whole task of eugenics is a continuous one. But the movement is under way and every future year should mark considerable headway, both in establishing the prin- ciples of eugenics thru analysis of facts, and in the practi- cal application of these principles thru education and the organization of custom. Finally, we conclude, in view of the evidence, that man achieved one of his major advances in history when he dis- covered that by applying certain essential principles, he could more purposefully direct his own evolution. INDEX Page A vitamin, association of copper with, McHargule ...................--..-.- 103 Achondroplasia in a family recently investigated —_...................... 214 Agricultural marls in Kentucky, S. C. Jones -.........22.2.2..2.222.222...- 154 Alcohol, apparatus for dehydration of, -..-.......2..02220.2.222222-22222-2--2--- 64 AmiphibialoL Kentucky, mMotesnon: - 2. /.-sae oe ye a 213 Anaphylaxis and hydrogen ion concentration, Healy, Spears and ES USER 5s eB aE a a Ae eR Celt 103 Anderson, W. S., Some problems in pauperism, ...................--------- 141 Anderson, W. 8S. and J. Holmes Martin, Unit characters in poul- LE Tes apres eal cae UNS UGA Se Ae ROaeaI SNA SA SINAN a UPON AMO La oa nth LR a 76 Application of science thru the agronomy extension service in DES 9Gey sl ere) by 0p a ged fe 0 a= cea een ela a Ee A i ea co tg 149 Association of copper with substances containing fat-soluble A VACATE Iie e IM CRTAT 2 UG ics -te ae. ett ae ans a Met le ede eel I Ih 103 Balanced selection in the fission rate of paramecium caudatum, AR VEC OT EE OME teks 22 a ee NE ae eels ted wes Tee thd 204 Bangson, J. S., Achondroplasia in a family -_-__......2..222222.222.2222.--2..- 214 Barbour, H. G., Falling drop method for specific gravity __.........- 213 Barrens, vegetation of, Harrison Garman ...._._._..........0.2222222222222.-- 107 Beckner, Lucien, Cumberland County Oil Horizons -...................... 23. Beckner, Lucien, The last wild pigeon in Kentucky .................... 55, “Big Lime” as a key horizon for structural work in N. E. Ky. 216 Big Sandy River, Major drainage of, W. R. Jillson ...................... 163 Bluegrass, Kentucky, whence did it come? J. S. McHargue...____. 179 Boyd County, Kentucky, exploration for oil and gas in, W. R. aI pIBLET SSG Ti tapgrat ees toes Cea = arate EU Peek Dee aah ee Or ow Mtns ACR uD rts Pn ee Sho euleee 139 Brauer, Alfred, The regeneration of the epithelium of the uri- MAG Va OLA Gen: se F250 selina 3 ee bla a eye 2 a a Geta 190 Brauer, Alfred, Micrognathy and accompanying anomalies in a FT ealryalo epeter eee eee ce at eee enn eee ni Scheie awh saan anew a ina ec es Oe ely Soe natant a 199 Bucher, Walter H., Subcrustal expansion as a possible factor in Carthediastrophismy-i.22.0.. 5.25. ste oe Oe 130 Buckner, G. Davis, The growth of chickens raised without grit... 178 Buckner, G. Davis, J. Holmes Martin and A. M. Peter, Calcium metapolismain “the layinge Wen 22. 2s ee ae 93 Burroughs, W. G., Geographic influences in the Ky. Knobs... 29 Burroughs, W. G., Geography of the Western Ky. Coal Field. 124 Burroughs, W. G., A newly discovered prehistoric fort _—_......._.. 2203 By Saw Sapere erent cee ewe VV SALE NOONE Sopa tee ae RS a a2 eo ge At 6 Calcium metabolism in the laying hen, Buckner .........................- 93 Caldwell, M. A., Some relations of science to philosophy _.......... 206 Canter; Caviesssre ology of, Wer Re Jillsom jets: ee ee oe ae ie Fin Carmin, Joseph, Physiological balance in nutrient solutions.____... 2 Chamberling er. Rollin ww. Harthquakes 222.2 ee ais Characteristic X-rays of molybdenum, T. M. Hahn __............._... 135 (CGP ANSH LUE EBSCO he a sc lg er ec eR NE Be er) 2 Cooperation, an experiment in, O. B. Jesness ~.__._..-.2222.22.22222 22222. 142 Copper, association of, with substances containing the fat-solu- DLE wACeVA CAT et Se MC Ear oe iSy eae cia lh Ue NIL Sst lca ela aU ll 103 Copper in mollusks and crustacea, J. S. McHargue __.___...... ep 49 Crouse, C. S., Present status of the oil shale industry _.............. 1esal Crump, Malcolm H., Harvard summer school of geology in 1875 64 Cumberland County oil horizons, Lucien Beckner _............-......... PAS Didlake, Mary, Tests of moth-proofing substances ...............-...... Zabel DixeRivier dam: iCONSEVUCtIONYOL. 20h Tey eee 39-44 Drainage area and waterway for culverts, etc., relation between 217 Drainage modification of the Big Sandy River, W. R. Jillson .... 137 Early glaciation in Kentucky, W. R. Jillson -..............-...-2-22.2.---.- Harthowakess Diss ollime wh) Cblaimilp erin sess eee eee Effect of diet upon the rate of learning and forgetting by domes- ticatederats; Ae Re Middleton ee ee Effect of the teaching of evolution upon the religious convictions of undergraduate students, A. R. Middleton —__--....-- bleventh ‘annual=mectine, minutes 0b =) = ee Epithelium, regeneration of, of the urinary bladder, Alfred IST ANWOT, seer Re ee Nee ee oes ee Te a Husenics, the principles of, EH. Ey Waughlim 222) 2) Evolution, effect of teaching, etc., A. R. Middleton -__.-__._........... Experience in collecting insects in a sink-hole, Harrison Garman Hxperimentanscooperation, Of B.) JieSWeSs sss te eee Exploration for oil and gas in Boyd County, Ky., W. R. Jillson__.. Falling drop method for specific gravity ___..-_____._.....5 2 Farmers’ earnings and standard of living in northern Keys. Weeb: INTC OM gies et Sek Peet a GE aM al Bie The TUS ye ss Mishes! Of sBooneiss Creek: eee 5 ee vn. n eis Fusarium species, inoculation of, W. D. Valleau -..-.......-.-.-..-...--.- Garman, Harrison, An experience in collecting insects in a sink- NOTE CAVGl ice lee, Ee Ee es ee leaner eee Garman, Harrison, Vegetation of the barrens -..-...-.....-.-...-.----------- Geographic influences in the Kentucky knobs, W. G. Burroughs Geography of the Western Coal Field, W. G. Burroughs -........... Geology of the ‘Carter caves, W. Ri Jillson 2.22.2 ee Giovannoli, L., Fishes of Boone’s Creek __.___.-..-.----2--22-2:-2022222 Glacial pebbles in Eastern Kentucky, W. R. Jillson -...-.............- Glaciation, carly, int Kentucky, W. Re Jillson ee Growth of chickens raised without grit, G. Davis Buckner ____.__. Hahn, T. M., Charecteristic X-rays of molybdenum __._____-.-....._-. Hamilton, W. F., Falling drop method for specific gravity _._.__.. Harvard Summer School of Geology in 1875, M. H. Crump .......- Healy, Laniel J., Spears and Healy, Anaphylaxis and hydrogen JOM CONCENMtTALIONG 5221. eet ee eee Hooper, J. J., Influence of season of calving on milk and butter PLO CAMUCHOMNORLCO WSs ate 2s a ee ee ee Ieloxeyoxenes dis dlgqr deneovebhevemyonaroye welkereaeanail Nie: Sel) ee ee Hudnall, J. S., Marine invasion in Eastern Kentucky in Pennsyl- VERT ELUM eteT NS ett es te re TEI ASSES Ce Sue Ss Hydroelectric development of Dix River. J. S. Van Winkle __.__.- Hydrogen ion concentration and anaphylaxis, Healy, Spears and I (=e Ly gem nents Oe Rea en reed ol De ee ed SR ee a pe Ne eer Inductance, simple method for determining, R. B. Scott —_..-..... Influence of season of calving on milk and butter production of COWS) HOODET 2802 Se eR Ee ee ee ee Iron, manganese and zinc in some moliusks and crustaceans, J. SoUIMIGHaT PUG. shat eh ee So Suna a sad stds Ai de cde anes aoe eee ee Jesness, O. B., An experiment in cooperation _..._...._...-.-....---.-.-.--- Jillson, W. R., Early glaciation in Kentucky ...._..........................- Jillson, W. R., Exploration for oil and gas in Boyd County, Ky. Jillson, W. R., Geology of the Carter caves ....._..-..-...-....-.------.--- Jillson, W. R., Geology of the oil shales of eastern U. S. ~....-... Jillson, W. R., Glacial pebbles in Eastern Kentucky |................... Jillson, W. R., Major drainage modification of the Big Sandy EU TIVG GIS! Ip ooo ete cn in a Ree 2g ier Ie ee eee ree Johnson, E. M. and W. D. Valleau, Preliminary inoculation ex- periments with fusariwum: Species 2.2. eee Jones, S. C., Agricultural marls in Kentucky -.........-. acl Sas Kenney, Ralph, The application of science thru the agronomy ex- COM SIO MM SOTVAC EM eyes eee eae ere en Se ees eee ae 149 Kentucky bluegrass—whence did it come? J. S. McHargue -.___... 179 Ken tuckyathonrobreds At. Ge 1h. Smit ne eee ibale Kiplinger, C. C.,; Dehydration of alcohol, ¢:.:.2_-2-2.2-..21.2 225: 64 Laboratory apparatus for the dehydration of alcohol. C. C. Kip- UY ate ees ae eis a, Pee beta cone iia dus ete ere pu eee ine, Ue See el ae 64 Lactobacillus acidophilus vs. Lactobacillus bulgaricus _............ 95 Last wild pigeon in Kentucky, L. Beckner . ....__...............-.--------.-- 55 lauchlin, Ho A. The principles of eugenics .2.22-2..22 2220 223 Lynch, J. T., Drainage area and waterway for culverts, ete. _____.- 21:7 Macintyre, Thelma, Amphibia of Kentucky —.2.2.-.5.2..:20) 2422 Dele McAllister, Cloyd N., Results of science teaching — in the schools 155 MecFerlan, A. C., West Fickman: ra wilite7 OMe. 2e8 ot ee Se eee es 214 McFarlen, A. C., mB TS MMe d aS aa kiG yar lOI17 OMe ss eee ss eter 216 McHargue, J. S., Association of copper with substances contain- Ine eEhestat=sOlbDlevwAs vitamins fe seek eel SO eS ee eee ase ae 103 McHargue, J. S., Copper, iron, manganese and zine in some mol- UISKSHaATIGd CRUSTACEANS shel 2 Ssircr se ea NA Ah Delian Ue aI Se a Hh 46 McHargue, J. S., Kentucky bluegrass—whence did it come? __..__.. 179 Major drainage modifications of the Big Sandy River, W. R. Jill- S ODE ee sec beni reste. GON EE Ste ney ake PRON UR ae imran hap eer tees eee St ea IBY { Manganese in mollusks and crustacea, J. S. McHargue ___.._......... 49 Marine invasion in eastern Kentucky in Pennsylvanian times, Dex EB UCo Eva eT) LU at aie hdr Ty ame ea Re ce a gga ee oem pn ee ees ee el) 44 Marls sAoricuitural;: in Kentucky, S:7C. Jomes 2%. 2 220 ee 154 Martin, J. Holmes, Buckner and Peter, Calcium metabolism in ING eye NO Ts reese eM ei nae ten ohh aoe 50, ini ae ANCE Wh aA dete dee ae Nae te ale 93 Martin, J. Holmes, and W. S. Anderson, Unit characters in poultry 76 VIE TIN CUCU weG G2 eee teas Sd ES le eee ied anus UALS eon ec ensayo Oe 9 Metabolism in the laying hen, Buckner, et al. ___.__.-.. 2202 22..0222222.L... 93 Metals occurring in mollusks and crustacea, McHargue __..__....__... 46 Micrognathy and accompanying anomalies in a lamb, Alfred BSCE AT CS SER es ee a el Pe ao SR SESE ee eee 199 Middleton, A. R., Effect of the teaching of evolution, etc. -_..._...... 172 Middleton, A. R., The effect of diet upon the rate of learning and LORSEtEIN Saye sCOMEStICALEd Tals eA ee ee ee 20/2 Middleton, A. R., Balanced selection in the fission rate of Para- TOQUE KERB Ua ow = OF: Fy DCG b> 0m D Una 0 eae pam ene tn Ly Ne Sloat Be eee 204 Milk and butter production of cows, influence of season of calv- INS ON i VacLLOODCY 0252 Ne ee plese Mae ene ce ae cok fue Dee 78 VMLKeE pM ROGUCEIONsOb Clea 222.2 ee ee ee Se ee seas Dies Mineral mixture as a supplement in hogging down COED, aHne J: EVV AINE ONT; Cl eager esi ae ne re fate clemaey R ee tht ES Aue eae ene a ea ee ee eee 119 Minwtes-of theeleventh: annual meeting, 2.0 15 Minwtessor the tweltth vannwal meeting, 22... 20 ee G9 NMinwtes;ore the thirteenth annual meeting (so. 2 see 165 Molybdenum, characteristic X-rays of, T. M. Hahn __._._........_._.- 135 IMIOSSC Ste Ole cent UG Keyae) Geico eee ee 56 Noth=prootine Substances: tTeStswOles 222 228 2h), ee ee Pal INS COI) O Sys sess ee a oe ah ee OB SEE LOY, ot ea ase ses ee nee ela 8 Nicholls, W. D., Farmers’ earnings and standard of living —_.__.... 57 Omicerskeotmthies Aca diem yaeo 52 lee SR ie ee ee See ean eo ae eens 4 Oil and gas, exploration for, in Boyd County, Ky., W. R. Jillson 139 Oilssandsvor- Cumberland), County, Wye ee 24-28 Oil shale industry, present status of, C. S. Crouse _____.-........... 13 Oil shales of Eastern U. S., geology of, W. R. Jillson __............. 226 Orizin of fibrous’ serpentine; lu: C. Robbins 222. 226 Pauperism, some problems in, W. S. Anderson __..............2....2.....- 141 Payne, V. F., Some electronic structural formulas ~..........-.......- Perry, E. S., Subsurface conditions in N. E. Ky. from well records Peter, A. M., Martin and Buckner, Calcium metabolism in the LRT TN 7 aN is Vn Soe Sear een en Se ee oN Petroleum, first discovery of, in Kentucky _......_.................-...-_.- Philosophy, some relations of science to, M. A. Caldwell __......._.- Physiological balance and antagonism in nutrient solutions for wheat, Sam Eo ‘Trelease, etal 2 Sen ee eae Pigeon, the last wild, in Kentucky, Lucien Beckner ...................- Prehistoric fort newly discovered, in Kentucky __......................... Preliminary inoculation experiments with Fusarium species, W. De Valleauand Hee Me Johnson) 2.2.2 ee ee eee Present status of the oil shale industry, C. S. Crouse ~__._......._.- Problems in pauperism, W. S. Anderson ................2.2222222.22-2-2-------- Regeneration of the epithelium of the urinary bladder, Alfred PB RAUCTY or eects ee tet a een ees ek Slane HC ny eee oe Sek A Relations of science to philosophy, M. A. Caldwell —_.................. Relation of some constituents of tobacco to grade, O. M. Shedd Resistance box, a universal, M. N. States: __.__....- 2.222.222 lees Robbins, L. C., Origin of fibrous serpentine __.__.-.....2..2.2.22.2222.2 2... Scherago, M., Origin of fibrous serpentine _..._.._..........-.2..22-22.2.-.--- Science, relation of, to philosophy, M. A. Caldwell ___...............-- Science teaching in the schools, the results of, Cloyd N. Mc- PATS EI eee Pees Pe OSes Bie SR ee Da Scott, R. B., Simple method for determination of inductance______.- Secretary7s: reports W923 -4 o.oo ee Secretany.sumeports (924-522 222 slosh ee eos a Secretary svinepo nye 92 5S Oise ae Be ee VS ae eileen Sula De gee ag en Shedd, O. M., Relation of some constituents of tobacco to grade Smith, George D., A Kentucky thorobred .......-...........2..2..2.2.2.-.------- Smith, George 'D., The mosses of Kentucky ~.._.....-..----2-.222 2-22-22... Some electronic structural formulas, V. F. Payne ..................... vey Spears, Howell, Healy and Healy, Anaphylaxis and hydroge 10D /CONCENtTAtION Leek se Ne ee States, M. N., A universal resistance box ...-.......2...2.-22.-.... 23a Status of the oil shale industry, C. S. Crouse ~.............2222.2.22.2......- Subcrustal expansion as a possible factor in earth diastrophism, Nia lter (ea aBUChier wien es Ue Woe Sac aie st 2 os inal alee eae ee Subsurface conditions in N. E. Ky., from well records, E. S. Perry Thirteenth annual meeting, minutes of —___....---.2222..22222 22222. Thorobred, A Kentucky;,:G. D. Smith’ 221 22.2 eee Tobacco, relation of some constituents of, to grade, O. M. Shedd Trelecse, Sam F., and Helen M., Physiological balance in nutrient SOLUEITOMS\: Voss! lores Seed eh Ue BG le ce Re oat eT ee oul eR eae ala a nea Unit characters in poultry, Anderson and Martin __.____................... Universal resistance box, A, M. N. States ~.._..-..222.22222222222 222222222222... Valleau, W. D., and E. M. Johnson, Preliminary inoculation ex- periments with Fusarium’ species, _._...<_.....). 2 eee Van Winkle; John iS2, 7 Dix River Diam crs ae ee eee eee Vegetation of the barrens, Harrison Garman ......-..............----.------- Western coal field, geography of, W. G. Burroughs -._____...._..._._.- West Hickman fault zone in Jessamine and Fayette Counties ___. Wilford, E. J., A mineral mixture as a supplement in hogging GOWAN CONT hee eG PDS Nn Ray iN URL es, Ts ica ee ee en X-ray, characteristic, of molybdenum, T. M. Hahn Yueh; €. (Ss, Dehydration of alcohols 3-22 ee eee Zinc in mollusks and crustacea, McHargue eTL' ; E AWAY : TRANSACTIONS KENTUCKY POADEMY OF SCIENCE AFFILIATED WITH THE A. A. A. S. VOLUME THREE (1927 - 1928) Fourteenth and Fifteenth Meetings LEXINGTON, KY. 1930 TRANSACTIONS OF THE KENTUCKY ACADEMY OF SCIENCE AFFILIATED: WITH THE A. A.A. Ss: VOLUME THREE (1927-21973) Fourteenth and Fifteenth Meetings This Volume was Edited by A. M. PETER and ETHEL V. T. CASWALL LEXINGTON, KY. 1691330 PRESS OF TRANSYLVANIA PRINTING CO. LEXINGTON, KY. W CONTENTS (EEC CIS epee Sa Sees ae A ae es Sue ie eee eS tee ar eres cate ee Fes CO TUS CUES EMO ine wee Se nas ed Ae VP 8s Fae Eee LUBE ROS ee tele ES Vex VES Mees ents le Sent Oe 29 Te Siu Phe atk Vie eee eel hd SINE ort as Ae ae soe i Pell ING CLOT Oey ie rit tae ree kact oe wren ea eee Ieee cries Bape eu eee AEE eh se eae EE tage ee eee IMG TMD GrSMMp OUT SiG e> 20 soe ee SS eS a ie Pe Seite e See a Miniiteston che L4th annual) mee time) se. eer ee ee eee Papers presented at the 14th annual meeting +222... ee Minutessotgchent>thsanimual: meetin eres: see ss ee ee ee Papers presented. at the L5th annual meeting -....22-.22..----2-2222 AT Gl Gee ete eh eee cE Se ate ee ate A Ae J eas 15 Kentucky Academy of Science OFFICERS 1926-1927 President, W. G. Burroughs, Berea College, Berea. Vice-President, Henry Meier, Sanger, California. Secretary, A. M. Peter, Experiment Station, Lexington. Treasurer, W. S. Anderson, Experiment Station, Lexington. Councilor to A. A. A. §., A. R. Middleton, Louisville. 1927-1928 President, W. D. Valleau, Experiment Station, Lexington. Vice-President, C. S. Crouse, University of Ky., Lexington. Secretary, A. M. Peter, Experiment Station, Lexington. Treasurer, W. S. Anderson, Experiment Station, Lexington. Councilor to A. A. A. S., A. R. Middleton, Louisville. CONSTITUTION AND BY-LAWS (As adopted May 8, 1914, and subsequently amended.) ARTICLE I.—NAME. This organization shall be Known as The Kentucky Academy of Science. ARTICLE II.—OBJECT. The object of this Academy shall be to encourage scientific research, to promote the diffusion of useful scien- tific knowledge and to unify the scientific interests of the State. ARTICLE III.—MEMBERSHIP. The membership of this Acad- emy shall consist of Active Members, Corresponding Members and Honorary Members. Active members shall be residents of Kentucky who are inter- ested in science, or other persons actively engaged in scientific in vestigation within the state. Active members are of two classes, national and local. National members are members of the Academy and of the American Association for the Advancement of Science, local members are members of the Academy but not of the Associa- tion. Each active member shall pay to the Academy an initiation fee, upon election, and annual dues beginning October 1 next after elec- tion, the amounts to be fixed in the by-laws. The amount of annual dues to be paid by a national member shall equal the difference be- tween the amount to be paid by a local member and the amount allowed per member by the A. A. A. S.. Any member in good stand- ing may become a life member by payment at one time of a suitable sum, prescribed in the by-laws, and is thereafter relieved from pay- ment of dues. Corresponding Members shall be persons who are actively engaged in scientific work not resident in the State of Kentucky. They shail have the same privileges and duties as Active Members but shall be free from all dues and shall not hold office. Honorary Members shall be persons who have acquired special prominence in science not residents of the State of Kentucky and shall not exceed twenty in number at any time. They shall be free from dues. For election to any class of membership the candidate must have been nominated in writing by two members, one of whom must know the applicant personally, receive a majority vote of the committee on membership and a three-fourths vote of the members of the Academy present at any session or, in the interim between meetings of the Academy, the unanimous vote of the members of the council, present or voting by letter. 6 THE KENTUCKY ACADEMY OF SCIENCE Article IV.—OFFICERS. The officers of the Academy sha}l be chosen annually by ballot, at the recommendation of a nominating committee of three, appointed by the President, and shali consist of a president, vice-president, secretary, treasurer, and councilor of the American Association for the Advancement of Science, who shall per- form the duties usually pertaining to their respective offices. Only the secretary, treasurer and councilor shall be eligible to reelection for consecutive terms. ARTICLE V.—COUNCIL. The Council shall consist of the Presi- dent, Vice-President, Secretary, Treasurer and President of the pre- ceding year. The council shall direct the affairs of the Academy dur- ing the intervals between the regular meetings and shall fill all vacancies occurring during such intervais. ARTICLE VI.—STANDING COMMITTEES. The Standing Com- mittees shall be as follows: A Committee on Membership appointed annually by the Presi- dent consisting of three members. A Committee on Publications consisting of the President, Secre- tary, and a third member chosen annually by the Academy. A Committee on Legislation consisting of three members ap- pointed annually by the President. ARTICLE VII.—MEETINGS. The Regular meetings of the Acad- emy shall be held at such time and place as the Council may select. The Council may call a special session, and a special] session shall be called at the written request of twenty members. ARTICLE VIII.—PUBLICATIONS. The Academy shall publish its transactions and papers which the Committee on Publications deem suitable. All members shall receive the publications of the Academy gratis. ARTICLE IX.—AMENDMENTS. This Constitution may be amended at any regular annual meeting by a three-fourths vote of all active members present, provided a notice of said amendment has been sent to each member ten days in advance of the meeting. BY-LAWS I—The following shall be the order of business. 1. Call to order. 2. Report of Officers. 3.1 Report of-Councils CONSTITUTION AND BY-LAWS 7 4 Report of Standing Committees. 5. Election of Members. 6. Report of Special Committees. 7. Appointment of Special Committees. 8. Unfinished business. 9. New Business, 10. Election of Officers. 11? “Program: 12. .§ Adjournment. II—No meeting of this Academy shall be held without thirty days’ notice having been given by the Secretary to all mem- bers. IJI—Twelve members shall constitute a quorum of the Academy for the transaction of business. Three of the Council shail constitute a quorum of the Council. IV—No bill against the Academy shall be paid without an order signed by the President and Secretary. V—tThe initiation fee for active members shali be one dollar. Annual dues shall be two dollars and fifty cents, for local members, and two dollars for national members. A life membership shali be fifty dollars. ViI—Members who shall allow their dues to be wnpaid for two years, having been annually notified of their arrearage by the Treasurer, shall have their names stricken from the roll. ViII—The President shall annually appoint an auditing committee of three who shall examine and report in writing upon the account of the Treasurer. VIII—The Secretary shall be free from all dues during his term of office. IX—All papers intended to be presented on the program or ad- stract of same must be submitted to the Secretary previous to the meeting. X—These by-laws may be amended or suspended by a two- thirds vote of the members present at any meeting. XI—The program committee shall consist of the Secretary of the Academy and the Secretaries of the divisions with the President of the Academy, ex officio. They shall serve from one annual meeting to the next. MECROLOGY Iu Memoriam They have crossed the river and are resting in the shade of the trees: Wayne Dickerson Iler, 1892-1928 Charles Joseph Norwood, 1853-1927 Louis B. Siff, 1865-1926 Stuart Weller, 1870-1927 MEMBERS 9 COMPLETE MEMBERSHIP LiST FOR THE YEARS 1926-27 AND 1927-8 c indicates Corresponding member; h indicates Honorary mem- * ber; 1 indicates Life member; indicates No longer a member; 7+ in- dicates Deceased. The date denotes the year of election to membership. Name and address Branch of Science Allen, W. R., ’23, University of Kentucky, Lexington -.__.-.......... Zoology *-Anderson, H. C., ’23, W. Ky. State Normal School, Bowling (Gara eee cs ae oe nh aE Sal poet Ulla es ue de Mie esi AAA ear Physics Anderson, W. M., 714, University of Louisville, Louisville -..._..... Physics Anderson, W. S., 715, University of Kentucky, Lexington ........ Genetics Averitt, S. D., 714, Experiment Siation, Lexington -............... Chemistry *-Baer, Louis, ’23, University of Louisville, Louisville ___._..... Chemistry IB Aker eA s Omi?! 216) IB CTC as: tes ce 2 er en Anthropology *-Bales, C. E., ’23, Louisville Fire Brick Co., Louisville -....... Chemistry c-Bancroft, George R., 719, Univ. of West Va., Morgantown, YUASA rat Boa rs Hsin og ne team ee ee Leo ey ea Chemistry Bangson, John §., 726, Berea College, Berea, -....2....-22....22222.c2e ee Biology Barbour, Henry G., 725, Univ. of Louisville, Louisville _....... Phvsiology Bassett, G. C., ’?27, Univ. of Kentucky, Lexington __._......._.... Psychology Bear, Robert M.,.’2:7, Centre College, Danville _.:2:...22:0.222220 Education iBecknerm lucien, 72:0) “Wamehester, 2:22 .-.02. Sees ee ee eee Geology *-Beebe, Morris W., ’23, Univ. of Kentucky, Lexington 2 TS Pt ee a gogo Sa ae POO aS ae Mining & Metallurgy Birckhead, E. F., ’28, Supt. City Schools, Winchester -.._......_. Hducaticon c-Blumenthal, P. L., 716, 316 Parker Ave., Buffalo, N. Y. ....Chemistry Boggs, Jos. S., 723, Waverly Apartments, Lexington __..__.__... Engineering Boyde e147 Univ. of Mentucky;, bexine tom: 2-2-5 22272 Mathematics -—pranhame~: J. O..0 24.° Massie school). Versailles 2.25 ae Science Brauer, Alfred, 9216, Univ. of Kentucky, Lexington. -2.22...2.222 Zoology Brown; I. ‘A., °?15, Experiment Station, Lexington ....2-22...2.-. Chemistry BRO WANING MN Giys Bi. 22. AS a rn iteou 2 ees = se er NT Se eee Geology e-Bucher, Walter, ’22, Univ. of Cincinnati, Cincinnati, Ohio....Geology Buckner, G. Davis, ’715, Experiment Station, Lexington _....... Chemistry Bullard, John F., ’26, Experiment Station —_._._............ _.....Vet. Science Bullitt, William Marshall, ’28, Inter-Southern Bldg., TOMS alll Ces eeensa ee SUR «oie ce Ws oe ene MOU Peleg Zens ee nae Gana ss soa Science Burrouchis® W....G.. 222° Berea, Collese; Berea 22) 22282 ee Geology h-Butts, Charles, ’22, U. S. Geol. Survey, Washingion, D. C..... Geology Caldwell, Morley A., ’15, Univ. of Louisville, Louisville _._._.. Psychology Capps, Julian H., ’28, Berea College, Berea ---.....-.2.----22---<-.---: Chemistry Carmichael, H. 'T., ’24, Ky. Asphalt Co., Kyrock; Ky _.-..2—: 10 THE KENTUCKY ACADEMY OF SCIENCE Caslickiih diwand Av 226. Claiborne. StudsePanis tas ee ees Vet. Med. Chalkley, Lyman; 72:2, Univ. of Kentucky, Lexington (:.23. 22 Law c-Clark, Friend E., ’15, Univ. of West Virginia, Morgantown, AV SV LY UT i Si eee AERTS Wide Zen ees EN AMES OE see Se Se ae Eee ee Chemistry Cooks Hh wailbur’ 228. Centres Collese. Danville 22-4 eee Biology *-Cooper, Dr. Homer E., ’26, Dean State Normal School, ECT CUT TYNO TG peor a See we Re A eee ek ee Education Cooper, Mrs. Clara C., ’26, Wallace Court, Richmond ....--.-._.. Education Cooper, Thomas P., 718, Director Experiment Station, TGS NAIVE EON aioe bers NON Maley mace ae yce eet Siler Eaceaty S A REO Rake ea Agriculture h-Coulter, Stanley, 714, LaFayette, Indiana, Purdue Univ. ......-. Botany c-Cox, Benjamin B., ’22, 855 Ontario St., Shreveport, La. -....... Geology *-Cox, Meredith, ’24, Eastern Ky. State Normal School, SEU TC TINO TAGE oS eae AN Yili ee ery ON Nig etait een od en Chemistry *-Craig, W. J., 720, W. Ky State Normal School, Bowling GTEC eee oot tee ee RO eed Doc ae See Lees Se Physics & Chem. Crooks. CG. 7b. “Centre iCollese, Danwille <2 - ee Mathematics Crouse: iC21S:, 1241" Univ of Wentucky,, lexington wakes Mining Eng. CECURTICT SE Di Winy 22:2) sO VGA CUS CA Nise LY cnc eee fe ol ae ee eee Geology Davies, P. A., ’26, University of Louisville, Louisville __._..._...._... Biology h-Day, Arthur L., ’17, Director Geophysical Lab., Washington, Pi Oe oh as POON eth or SN ators teen AINE Ans eet, «SATS 3 NA A NUE Ee paew nme Geology h-Detlefsen, J. A., 718, Wistar Inst., Philadelphia, Pa -...-....... Genetics Didlake, Miss Mary L., 714, Experiment Station, Lexington SP eh UA Se WN Saas tes SENG IR te end AL aes ent, DR NLT eh Ent. & Botany Dimock, W. W., ’20, Experiment Station, Lexington _...._._.. Vet. Science *-Downing, H. H., ’°14, Univ. of Kentucky, Lexington _.___.. Mathematics Edwards, Philip R., ’26, Experiment Station, Lexington _...Vet. Science Erikson, Miss Statie, ’26, Univ. of Kentucky, Lexington...Home. EHcs. Te Hive eV aN wi Ge ela. UCR TS tO Mie tens aes 22. oa ee Geology Rehn: “Arthur R., ’?24, Gentre College, Danville 1.22272 Mathematics Meresus, HW. N.,' ’21, Experiment ‘Station; Lexington: 12.230 Agronomy Flexner, Dr. Morris, ’26, Francis Building, Louisville —.__........ Medicine Hoerster, M. H.,.’16, Consolidation Coal Co.; Jenkins 2227-2 Forestry c=-hohs. He drirlius: 715,460 Broadway. New York, No Yo =] eae Geology Ford, M. C., ’23, W. Ky State Normal School, Bowling GrCGne pir red DE NMR eh Re Re eS rane oe Na et Agriculture Frank, Dr. Louis, ’26, The Heyburn Bldg., Louisville --...:...... Medicine Funkhouser, W. D., 719, Univ. of Kentucky, Lexington ...........- Zoology ce-Gardner, J. H., 7215, 505 Exchange Nat. Bank Bldg., Tulsa, OR Tahoma, | seein i ae a Pals hoe Nee 5 Sia eae Geology Garman, H.,.°14, Experiment: Station, Lexington 3.42.2 Biology *-Gentry, H. V., ’23, Louisville Gas & Electric Co., TE OUTS VAT Be ne iE a a Sa ple SEER ECR SO ANd hae nin ee eee Chemistry Giovannoli, Leonard. ’26, 162 N. Ashland Ave., Lexington........ Zoology MEMBERS ab h-Glenn, L. C., ’22, Vanderbilt Univ., Nashville, Tenn -.-......... Geology Good, E. S., ’214, Experiment Station, Lexington ................ Animal Hush. fam Gr OUtster Hasire t ried Gis. Ls OX TING OM: eee ee ee Bad oe a teat ae Bacteriology Graham Charles-C:, 725, Berea, College, Berea’ 2222222 2 Science Graham, James L., ’27, Univ. of Kentucky, Lexington ........ Psychology *-Grinstead, Wren James, ’21, Univ. of Penna., Philadelphia, ET O10 01s pS Se see Tc Pea an A Psychology Guilliams, Jonn Milton, ’25, Berea Normal School, Berea....Mathematics l-Guthrie, Dr. William A., ’26, So. Ky. Sanitorium, 1 ENT p€215 a] el aT epeenee rele pe en ee e e RAIL RUN oe eed eC oy Med. Science Hamilton, W. F., ’26, Univ. of Louisville, Louisville -.........2..... Geology Harms, Miss Amanda, 719, Experiment Station, Lexington _...._.. Biology h-Hart. He, Bz, 7195. Univ. of Wisconsin, Madison, Wis. .....-..-2 Nutrition c-Havenhill, Mark, ’19, 629 Poirier St., Oakland, Calif..... Healy, Daniel J., ’14, Experimenz Station, Lexington -....... Bacteriology c-Hendrick, H. D., ’214, Takoma Park, Washington, D. C......... Agronomy Hendricks, PA: "275: Berea College, Berea 2:2.422.4 222i0cx Hinton, Robert T., 714, Georgetown College, Georgetown .......- Biology FainewChariles;, 92.8. state Normal School, Murray 22... Physics Homberger, A. W., ’19, Univ. of Louisville, Louisville --.. _.Chemistry Peet NO Ol) Ciara ike (hal ee tee aude ae i tM Pet Rela Biology Hopkins, Miss Mariel, ’26, Univ. of Kentucky, Lexington...-._-. Home KEcs. Hudnall wames! S.5 021, Coleman, Texas 22x ee ee Geciogy Aniline Wo 726. Hxperiment Station, Lexington: ~...2%.....22 Vet. Science Hutchins, William J., ’25, President Berea College, Berea _.._.Education +-Hler, W. D., 718, Experiment Station, Lexington...-_.-....-..-..._- Chemistry =“Ingerson, MW. J., 23, Céntre College, Danville: 2.2.2. .222..22- Geology =—invines George... 24, iy. Utilities: Co... Danville... ————__—_—_. Jewett, H. H., 21, Experiment Station, Lexington -.-...._._.. Entomology I=Iilsony We Rs, 719, State Geologist; Mrankforte.2 12210 2 Geology Johnson, H. M., ’25, Experiment Station, Lexington ............ Agronomy Jones. S.C. 714" Hxperiment.. Station, (WMexingtom 2.2682..." Azronomy Karraker, P. E., 715, Experiment Station, Lexington —__....._.... Agronomy c-Kercher, Otis, 719, Pike Co. Farm Bureau, Pittsfield, II11.-_.. Agriculture *_Killebrew, C. D., ’715, Alabama Polytechnic Inst., Auburn, PACE ETI ely ote sehen Lan Sees Sie au ny MINA Ae Bene geen ae OEE Physics King, Miss Effie, ’25, Morehead State Normal School, HIVES Te 2a Ui aise re Re eer eres OM Sola al ok Se Rothe es eie be Biology Kinney; J. 715. Mxperiment. Station, -Wwexington) 2.22222: Agronomy e-Kiplinger, C. C., ’718, Mt. Union College, Alliance, Ohio _.... Chemistry caKknapproR: Ha 4 2939" Clit. St. sane Mlero,1@alib. aa Bacteriology *_Koffman, Gladstone, ’23, Principal High School, Frankfort.__._. Physics KoppiusssO. Ta. 225. Univ. of Kentucky, lexington. Mee Physics Kornhauser,’ S21.,:’23: Univ. of Louisville, Woulisville 220.5. Anatomy 12 THE KENTUCKY ACADEMY OF SCIENCE Mame sR Cand 64Colemrane “Mex asies iieeis 120i, Vallis Vee inl bee enema Geology I OLEKS Ue haabe pend eac teed alike io ilyax=i avo pet oN) We eennea tn ean Mn EEOC Sa MRED AI te Geology c-Leigh, Townes R., 719, Univ. of Florida, Gainesville, EEO TAG aig eee Neca SUS ne AB esa Ie rh Ws eee he UL ee Chemistry Lester, William J., 726, Russellville, Arkansas -....-......2.....- LeStourgeon, Miss Elizabeth, ’24, Univ. of Kentucky, Ts SRT OTS Vg g See eet ete ane Len ee eel Oona s BAN. RENUETE ROI dO Mathematics *-Lewis, Charles D., ’15, Dean, Morehead Normal School, INE OTC TSE Clty pee cet Ca i hy eases ee INE a Uv ee oe Re a Nat. Science Ligon, M. E., ’28, Univ. of Kentucky, Lexington .....-........2...-- Education Lynch, John T., ’26, Road Engineering Dept., Frankfort ....Engineering McAllister, Cloyd N., ’?17, Berea College, Berea .-........2.......... Psychology McCormack, A. T., ’20, State Board of Health, Louisville _..Sanitation McFarlan, Arthur C., ’?24, Univ. of Kentucky, Lexington .......-.... Geology *-McFarland, Frank T., 714, Univ. of Kentucky, Lexington _....... Botany McHargue, J. S., 714, Experiment Station, Lexington —......._... Chemistry MacIntyre, Miss Thelma, ’26, Springfield, Ky. ---......................--- Zoology = VMckunnon, Masse Team. CsiOii Maes yy fo ee el ee aes Home Kes. McNamara, Miss Catherine B., ’25, Geological Survey, Byam h Otis. yet oe ck eat ae a Gite 2. BE ea Geology MeVey, Frank L., 718, President, Univ. of Kentucky, Lex- PIV EG OW ooteeet ota. O8 eM 2S a see Rt eee ao Le Ne Economics Marshall, Matcolm (Y!, MiD 3.227," Elendersom) 22.228 sien Medicine Martin, J. H., ’15, Experiment Station, Lexington -.--.-._.-_.._.- Chemistry Mathews, C. W., 716, Univ. of Kentucky, Lexington ____...___.. Horticulture Mayfield, Samuel M., ’23, Berea College, Berea __.._..__..-.... Nat. Science Meader, A. L., ’23, Experiment Station, Lexington_._._...._._._.- Chemistry Meier, Henry, 715, 1820 Date Ave., Sanger, Calif. ...Math. & Astronomy Middleton, Austin R., ’22, Univ. of Louisville, Louisville ._..- ---Biology Miller, A. M., 714, Belleview Cottage, Sunset Drive, Asheville, TING Seta La a GRR oS Sly SE eine soe a alates ae rd fu Ie ue ee ae Geology h-Miller, Dayton C., ’715, Case School of Applied Science, Cleve- VEVINTs MOTI OG Mie oo tees ENS AME 1 HS cal Ces eye 2 pe Physics Miller Je Wa, 723, Univ: of Louisville: Louisville (2s Medicine Miller, Raymond, ’26, Univ. of Kentucky, Lexington -............... Geology Miller WeiBy rons 12 2°n Wallis i @neekiiecs 20 tones eet euaaae dngineering h-Millikan, R. A. ’20, Calif. Inst. of Technology, Pasadena, EDT ith ee oe RN Lie MN SAT es UI A ti Physics Miners: J: By ?22.Univvot Kentucky, lexington 2222 oe ee Psychology c-Morgan, Thomas H., ’15, Columbia University, New York........ Biology h-Moulton, BOR. 716, Univ. of Chicago, Chicago; Tl) 23222 Astronomy Munroe, Donald James, ’28, Sun Oil Co., Dallas, Texas..__..._...- Geology SING WGOM ee RDU NG ee alu OU Renee AIL RE ol, UU I OR a Chemistry Nicholls, W. D., ’?14, Univ. of Kentucky, Lexington ..............-- Farm Ecs. Nickell, Clarence, ’25, Morehead Normal School, Morehead _...Chemistry MEMBERS 13 c-Nollauw Hb. Hi. 714. Norton'St:,, Newburg, N. Y..2-2..22272 22: Chemistry Norton, Mrs. Charles F., ’27, Transylvania College, Lexing- TEC Tae es a Pe RUE hehe Sok aN ie one Teed a NE CE 1a on PEMISE Tigges ote CeRue. po Library +-Norwood, C. J., 714, Univ. of Kentucky, Lexington........._...... fee a OG et STR kA as I ES Mining & Metallurgy O’Bannon, Lester S., ’23, Univ. of Kentucky, Lexington _...Engineering Olney, Albert J., ’20, Univ. of Kentucky, Lexington -......... Horticulture Parker, George H., ’26, Ky Actuarial Bureau, Louisville_..__. Engineering Payne, V. F., ’24, Transylvania College, Lexington --::.:.--.2..2.- Chemistry zohence,.Me i... 14. Unive of Kentucky; bexington: 2.1.02 Physics Peter, Alfred M., ’14, Experiment Station, Lexington —__..._.. Chemistry Pierce, J. Stanton, ’26, Georgetown College, Georgetown ...... Chemistry SPT CheMNVs Oumaaon ND amv Gates ots. SU SEE 2 ay a be eee: Physics *-Porter, R. E., 723, Ashland Leather Co., heed OE AE AS cote Chemistry Posey, M. E. S., 725, Dept. Roads & Highways, Frankfort—Engineering PGLyOL «ds W., 4, Univ. of Kentucky, lexington .222..4 22.2 Physiology Pyles, Henry M., ’26, Wesleyan College, Winchester _............... Biology Rainey, F. L., ’14, Centre College, Danville -............. Biology & Geology *-Rees, H. L., 714, Univ. of Kentucky, Lexington ................ Mathematics Rhoads, McHenry, ’21, Supt. Public Instruction, Frankfort....Education Rhoads, Wayland, ’22, Experiment Station, Lexington ....Animal Husb. h-Richardson, Charles H., ’22, Syracuse Univ., Syracuse IN ae eae Orr ee Erin me A i Rik hh DAL IE Ao I ae CUES eae Geology NaRvesheeioe Oormell UWitive. Wbivarcay IN Yulee 2. ees eee Geology Roberts, George, ’14, Experiment Station, Lexington ___------.- Agronomy c-Roe, Miss Mabel, ’19, 257 Roswell Ave., Long Beach, Calif. PRR RM Ee NE eR tn Mi LRN Ns ae SIS Pee Plant Pathology *-Rogers, John C., ’22, Lab. Preventive Medicine, Univ. of @lMi Calo ORME Ss Am ce eas et eS BON ns ths eh) oe Pees Medicine Routt, Grover C., 714, County Agent, Mayfield, Cree Dope tee (BUTE Eyes ase NS oe AUN ae cat ae ME td Sa eee see LOM wets a Biology c-Ryland, Garnett, ’14, Richmond College, Richmond, Va.....Chemistry *-Sandstrom, W. M., ’23, Univ. of Louisville, Louisville -._.-- Chemistry Saunders Jeni 7 2'5..33'9 Park Ave. dueximetoml 2.22) .2222.2 2: 2 *Scherago, Morris, ’23, Univ. of Kentucky, Lexington -..-.. Bacteriology Schnieb, Miss Anna A., ’26, E. Ky. State Normal School, RHC Tria TN ee eel enema cee QE Ol ics eee ER Salve 2 Ua ea ease 3 Psychology Scott, Miss Hattie M., ’25, Ky. Geological Survey, Frankfort ....Geology Shelton, William A., ’25, Principal High School, Vine Grove....Education Shephard, Nat L., 728, Franklin Fluorspar Co., Marion....._.. Chemistry +-Siff, Louis, ’15, Univ. of Louisville, Louisville -............... Mathematics Smith, George D., ’20, E. Ky. State Normal School,.............-.- J BUTT(ell ala a a1 Oy 010 beaut geet es ee eae et ea eg eg det ed ay eae Nat. Science C-SmithoN. bh, Citadel Collese, Charleston, S. (C.- .2--....- Physics 14 THE KENTUCKY ACADEMY OF SCIENCE h-Smith, William Benjamin, ’23, 9 Price Ave., Columbia, MUO SF 02s oe a Nee celta aR Eee GES SO ae eae oe aS oe Ee eee Philosophy Solomon, Leon L., ’20, The Solomon Clinic, Louisville -_...______.- Medicine South, Lillian H., ’20, State Board of Health, Louisville -.- ee ern Shea Oo Sunn sate Pelee Ae ea ies Jo LON see SOS Bacteriology CaS Dar Ria eles a ye ee ees Ne Ge Physics states, MN. 17%, Univ.cof Kentucky, Lexinzton 2222.29 Physics e-Stiles, Charles F., 714, A. & M. College, Stillwater Okla. -... Bie a eae oe re Rete LIS Aantal hers oa Me esas aces te set Se oe ng eee Entomology Strandskov, Herluf H., ’25, Univ. of Louisville, Louisville _.. SF ee ae I ei PELE EIS eS Ae a Rah YS DP I PR Ce Plant Physiology Suter, Arthur Lee, ’20, Suter’s Drug Store, Washington, D. C. eases foe fos wuts t cack) sewed en ee, Sy Be sine) Soe Seb ite cases yaa nea seuer mea Pharmacology c-Tashof, Ivan P., ’14, 724 Ninth St., N. W., Washington, Le) ied Oty wre reteset ne en ae 7 eee eee SMa aos Mining & Metallurgy Taylor, L. W., ’28, Experiment Station, Lexington -...._....._--2:.... Poultry Taylor, William S., ’26, Univ. of Kentucky, Lexington ........ Education Terrell, Glanville, °27, Univ. of Kentucky, Lexington ........ Philosophy Threlkeld, Miss Hilda, ’27, Dean of Hamilton College, Lex- ATS COM + - osc es ee a aas sea de cts coe es ee ee Education Thruston, Re CeuBallandsy: 15, AuOUiSivallll Gis 2. 5 tee. eee eee Geology Todd, E. N., ’25, Dept. Roads & Highways, Frankfort ........ Engineering *-Trelease, Sam, ’24, Columbia Univ., New York, N. Y. .... BAIL oe 2 oe Ben pees A eee MND oat ody CEU Jee BOE eee em Plant physiology *-Tuttle, F. E., ’14, Univ. of Kentucky, Lexington __.__.-.._..... Chemistry Valleau, W. D., ’20, Experiment Station, Lexington _...Plant pathology Van Slyke, Edgar, ’26, Centre College, Danville -...............222.... Biology Van Winkle, John S., ’24, Centre College, Danville ~.............. Geology Vaughn, Erle C., ’714, Experiment Station, Lexington __... Ent. & botany Walker, William H., ’26, Berea College, Berea .......-............ Psychology h-Ward, Henry B., 221, Univ. of “illinois, Urbana, Til eee Zoology eeWaush. Karl, 22:3, Berea: College, Berea. 2 2... 2s eee Psychology *-Webb, William S., ’714, Univ. of Kentucky, Lexington _..._._._.. Physics Weidler, Albert G., ’27, Berea College, Berea ..............-....-.-...--- a T-VWieller Stuant,-2e, Univ. of Chicago, Chicaeo: fn. =eae Geology Will) Re 'G:. 222.8" Centre. College: Danviller 2.2. eee Psychology *-Williams, A. B., ’24, Ky. Geological Survey, Frankfort..-....... Geology Williams, Charles W., ’23, 215-25 Central Ave., Louisville __.Chemistry =oWilson, A. BH. “24. 41° S: dath St.. Richmond> ind 22s ge MNS a Err a l Pepe OM ae rae aah LOE Geology & Zoology Walson:: Gordon, 72,7, Bowling (Green 2222-2 eee Wilson, Samuel M..)72'6.) Lexington] 22, 2 eee Law Wurtz, George B., ’28, Weather Bureau, Lexington __.....- Meteorology WyckoOfi.. IRs DYSON. 72107 yie oo ee ee th, Se See ne ee Education FOURTEENTH ANNUAL MEETING 15 MINUTES OF THE 14th) ANNUAL MEEZING, MAN 7; 1927 The meeting was called to order by president Burroughs ate cia Oo clock, A. M., im-room 200 of the Physics: Building, University of Kentucky. Present, about 30 members. The President announced that Dr. Middleton’s Presidential address of last year, on the effect of the teaching of evolution on the religious convictions of undergraduate students, had been printed in book form and that Dr. Middleton had brought copies for distribution. Treasurer Anderson presented his report showing a balance of $369.62 in the treasury and $51.00 invested to cover a life membership. On motion the report was received and referred to an auditing committe, composed of Dr. Boyd, Dr. Middle- ton and Mr. Payne. Secretary Peter presented his report in outline. Upon mo- tion it was accepted. Dr. Buckner moved that the Academy prepare resolutions on the deaths of Prof. Norwood and Dr. Siff, Academy mem- bens;.,to be spread ‘on the minutes and sent to the, bereaved families. Seconded by McHargue and adopted unanimously. The resolutions follow. PROPESSOR CHARLES JOSEPH NORWOOD The death of Dean Norwood has caused the Kentucky Academy of Science to lose one of its most valued members. Professor Norwood, aged 73 years, Head of the Department of Mines and Metallurgy of the College of Engineering of the Unt- versity of Kentucky, died at his residence in Lexington, Ken- iucky on) January 20th, 1927. Charles Joseph Norwood was born at New Harmony, In- diana, on September 17, 1853. The son of a distinguished sci- entist, Joseph Granville Norwood, he inherited characteristics FOURTEENTH ANNUAL MEETING 1 a which placed him in the front rank as a teacher, scientist and leader"of.men. He received his education at the University. of Missouri and under private teachers. He was given the degree oOmviaster of Science, in 1906; by the Keritucky, State! College: now the University of Kentucky. After many years spent as a geologist and mining engineer, Professor Norwood came to the University of Kentucky, in 1901, as professor of mining engineering. In 1911, Professoi Norwood became Dean of the College of Mining and Metallurgy, a position he held until the consolidation of the engineering colleges in 1918. Professor Norwood was a teacher of the highest ability and produced many valuable publications which record his contrt- butions to science. He was instrumental ima large measure in developing the coal mining industry of Kentucky. The Kentucky Academy of Science mourns the loss of one of its most valued and respected members. DOCTOR) LOUIS;SI EE Byte death “or, Wector Louis Siff, Professor, of -Mathe- matics in the University of Louisville, on December 26th, 1926, the Kentucky Academy of Science has lost a valued member. Louis Siff was born in Kasien, Russia, in the year 1865. He was the son of a Rabbi and his early education was with the view of following his tather’s calling. At the age of 19 he came to America, having had an education well grounded in languages by private tutors and the Rabbinical College at Ra- sien. He studied at Worcester. He was a student of the high- est order, spoke several languages, read several more and took special pleasure in the study of mathematics. Somewhat later he moved to. New York City and studied music. Professor Siff had held the professorships of languages in the University of Maine and in Union College, Barbourville, Ky., before accept- ing the chair of mathematics in the University of Louisville. This latter position he held at the time of his death. Professor 18 THE KENTUCKY ACADEMY OF SCIENCE Siff was a serious-minded man, studious and conscientious in his teaching. The report of the Council was read by President Burroughs. The report of the Membership Committee was read by Chairman Payne. Upon motion, the report was adopted and the Secretary was ordered to cast one ballot for all the nominees. Accordingly, the following named persons were declared duly elected active members of the Academy: James Llewellyn Leggett, M. A., Professor of Psychology and Education, Transylvania College. Mrs.-Charles F, Norton, A. B., Librarian, Transylvania College. Miss Florence Schcenleber, A. M., Professor in Home Econom- ics, Hamilton College. Elmer Elsworth Snoddy, M. A., Professor of Philosophy, Tran- sylvania College and The College of the Bible. James Clyde’ Vannetter, A. B., M. D. Assistant Protessonpor Biology, Transylvania College. Miss Hilda Threlkeld, A..B., Dean of Hamilton College: The President appointed as nominating committee Messrs. Jillson, Crouse and Buckner, to report nominations for officers of the Academy at the afternoon session. The Publications ‘Committee, reported thru Dra; Petenmthar part of.the manuscript for Volume II. of the: Transactions had been given to the Tas. M. Byrnes Company for printing. This volume will cover the 11th, 12th, and 13th meetings. Dr. Middleton gave a short report on the meeting of the A. A. A. S., in Philadelphia, which he attended as representa- tive of the Academy in the Council of the Association. Dr. Jillson announced that he had brought a number of publications of the Kentucky Geological Survey for distribu- tion to any one wanting them. FOURTEENTH ANNUAL MEETING 19 President Burroughs read his presidential address on “The prehistoric forts of Kentucky.” Dr. Oscar Riddle, by invitation, gave an illustrated lecture on “Studies on the thyroid glands.” At 10:45 the general session adjourned until 2 P. M. and the Divisions assembled in separate rooms for reading papers. ihe senetal session was called to order'at.2 P.M. Dr. Martin Hz Fischer, of the Untversity of :\Cincinnati delivered an address on “The constitution of living matter.” The nominating committee reported as follows: For President, W. D. Valleau. For Vice-President, C. S. Crouse. or secretary, AY M. Peter: For Treasurer, W. S. Anderson. Councilor to the A. A. A. S., A. R. Middleton. Upon motion, the report was adopted and the Secretary was ordered to) cast one ballot tor the nominees. | They were then declared elected unanimously. The Divisions reported election of officers as follows: Biological sciences—G. D. Buckner, President; E. N. Fergus, secretary. Physical and Mathematical Sciences—W. R. Jillson, Bresident;C: 5. Crouse, Secretary. Philosophy and Psychol- ogy—M. A. Caldweli, President and Secretary. A telegram was read conveying a greeting from the session ortthe: North. Carolina. Academy, Phe Secretary was’) directed to send the following reply: “Appreciate your message. Ken- tucky Academy sends cordial greetings to the Carolina Academy.” There being no further business, the Academy adjourned sine die. bo THE KENTUCKY ACADEMY OF SCIENCE SECRETARY'S REPORT, 1926-7 The President appointed the following committees to serve during the year 1926-7: Memberhip: V. F. Payne, Transy!- vania College, Chairman; E. C: Vaughn; Experiment -Station, and Lucien Beckner, ‘Winchester: Legislation: W. R. Jillson, Frankfort, Chairman; J. S. Me- Hargue, Experiment Station, and John S. Bangson, Berea College. Of the 23 persons elected to active membership at the last meeting, 18 have paid the initiation fee and have been added to the roll.of the Academy. Besides these, since the meeting, 3 have come in thru the A, A. A. S. (Krank, Lester-and Sclniep)) and 8 were elected by the council (Terrell, Boynton, Graham, Bassett, Bear, Hendricks, Hatcher and Weidler), 4 of whom have paid the initiation fee and have been added to the roll. We have lost 2 members by déath: - Prof. Charles J. Norwood, of the University of Kentucky, and Dr. Louis Siff, of the Univer- sity of Louisville. The total membership is now 174, including 86 national and 51 local members, making 137 active members, besides 23 corres- ponding members and 14 honorary members. The membership may be classified as follows: Active members in good standing, including 2 life members. 101 Active members in arrears. Wyent ite. Nala ae 24 ANCEIVe MEMIDERS Mr achears 2 Wears wee). catia cult eee 2 Corresponding, Vnrenabersy ioe se Nat ine ae eee 23 TIOHOPAT WMD GES {RI el eI a AA on A Oe A 14 174 Number ot members at last meetiie (1926) 20 ae 179 Dropped fromthe roll/tor all reasons. ee 30 149 New | Mienibers added i.e) wie i RN ie ie Sa) ee 25 FOURTEENTH ANNUAL MEETING 21 Classified geographically and as to educational institutions the active membership includes: 95 from the University of Kentucky, Lexington, 9 from the University of Louisville, Louisville, 11 from Berea College, Berea, 5 from Centre College, Danville, 5 from Eastern State Normal School, Richmond, 3 from the Western State Normal School, Bowling Green, 2 from Morehead State Normal Schoo!, Morehead, from Georgetown College, Georgetown, 2 1 from Transylvania University, Lexington, 1 from Massie School, Versailles, 1 from the Kentucky Wesleyan College, Winchester. Not connected with educational institutions in the state are 8 from Frankfort, 8 from Louisville, 3 from Lexington, 2 from Berea, 2 from Ashland, and 1 each from Owensboro, Middles- boro, Kyrock, Winchester, Paris, Jenkins, Danville, Wallins Creek, “Mayfield and Vine Grove. Besides these there are 5 active members outside the state. Your Secretary had some correspondence with Dr. Gerhard Dietrichson, of the Massachusetts Institute of Technology, Secketary or ocection © of the A. ALA. S.,\1in tegard to the pro- Siam con tne Chemistry Section ot the A. A. A‘. S for the) Phila- delphia meeting. The Council approved the program by letter vote. The council has considered the advisability of forming a division of philosophy and psychology of the Academy (this be- ing left to them for decision by the last annual meeting) and has appointed Dr. M. A. Caldwell, of the University of Louis- ville, as Secretary of the division, with instructions to prepare a program and organize the Division at the coming meeting. The matter of making this a physics meeting memorial to Isaac Newton, the 200th anniversary of whose death occurs this year, was proposed by Dr. Boyd and taken up by the council. This was not approved. 22 THE KENTUCKY ACADEMY OF SCIENCE Two meetings of the Program Committee were held in Dr. Peter’s office. No meetings of the Council were held but busi- ness transacted by letter. A letter was received from A. G. McCall, Executive Secre- tary of the First International Congress of Soil Science to be held at Washington; D.C., Tune 13 to 22,1927, askinew toned committee to cooperate with the general committee. The presi- dent appointed George -Roberts, Chairman; P. E. Karraker and 2S. Merlareue: In December a letter was written to our Senators in Wash- ington urging them to do all they could to help forward the passage of the McNary-Woodruff Bill providing for increasing the acreage of the National Forests in the Eastern part of the United States. The Secretary took up with Dr. Livingston the matter of the National Association sending a delegate to the meetings of the Academy, to which Dr. :ivingston replied: at slengthyae” delegate was not appointed. As noted in the minutes of our last meeting, Governor Fields ‘appointed Dr. W. R. Jillson to represent :the Statevor Kentucky at. the 14th’ International Geological Congress vat Madrid, Spain, May 24 to 31, inclusive, 1926. Dr. Jillsonpat- tended and also represented the Kentucky Academy. Dr. Austin R. Middleton, of the University of Louisville, attended the council meetings at the Philadelphia meeting of the A: A.A. S: last December, as representative of the Academy, A letter was received from Hon. Samuel M. Wilson invit- ing the Academy to join in the celebration in memory of Henry Clay and Thomas ‘Jefferson, on April 12, 1927, in Lexington: The Secretary sent the telegram to Hon. Randolph Per- kins, as instructed in the resolutions, and also wrote letters of thanks to President McVey and to Dr. Laughlin. Respectfully submitted, Al Mo PA WEIR. Seeretaiye FOURTEENTH ANNUAL MEETING 31 Discusses certain chemicophysical effects of high pressure, its direct effect on germination, and on the percentages of soit and hard seeds. The application of 2,000 atmospheres hydraulic pressure to seeds of Medicago sativa (alfalfa) increased the total germination over 50 per cent when the seeds were dried and were germinated after 30 days and after 6 months. Appli- cation of 2,000 atmospheres pressure between 5 and 20 minutes to seeds of Jelilotus alba (sweet clover) increased the total germination over 200 per cent when the seeds were dried and stored for 30 days before the tests were made. An increase of over 150 per cent in germination was obtained with seeds from the same bulks when the seeds were dried for 6 months before the germination tests were made. The application of 500 atmos- pheres hydraulic pressure for 2 and 8 hours failed to produce the high percentage germination obtained with 2,000 atmos- pheres. Short exposures at high pressures are more advaii- tageous than long exposures at low pressures. For a full ac- countvor the work see Jour. Gen. Physiol.,, Vol!-9; pp., 805-9, 1926, and Amer. Jour. Botany, Vol. 15, pp. 149-56 and ‘pp. 433-36. 1928. 6. Breeding Red Clover. E..N. Fergus. Ky. Agricultural Experiment Station. Red clover is a decidedly variable plant. From being grown in different localities for many years numerous geographic varieties have arisen. These varieties often show some mor- phological differences, but exhibit greater physiological varia- tions. Consequently, the yield of clover grown on the Station farm from different lots of seed has ranged from nothing to about 2% tons of mow-cured hay to the acre, under uniform conditions. Photografs were exhibited showing the differences described. An attempt is being made to further improve adapted strains by modern methods of crop breeding. 7. The Effect of Chlorides in the Fertilizer Application on the Chlorine Content of Burley Tobacco. P. E. Karraker. Kv Agricultural Experiment Station. By title. bo THE KENTUCKY ACADEMY OF SCIENCE Cs 8. A Chemical Analysis 0: the Bean and Pod of the Ken- tucky Coffee-Nut Tree (Gymuocladus canadensis). C. A. Bar- kenbus and A. J. Zimmerman, Chemistry Department, U. of Ky: Besidés the chemical analyses of the bean and podmthe physical and chemical constants of the oil were reported. For a tull account of the work see’ four. Am. Chem, Soc. Vols? pp. 2061-64. 1927. 9. The Mountain Flora of Kentucky. Geo. D. Smith, East- ern Kentucky State Normal School. Exhibited many fine lantern shdes of wild plants, and com- mented on them. 10. An Organism Isolated From the Feces of a Colitis Patient. - Earle’ K. Borman. Ky. Agricultural, Experiment Station. The organism was first thought to be Salmonella morgant but differs in producing acid in sucrose broth. Further work is planned. 11. Geology of the Island Creek Oil Pool.* (Abstract) Won. jillson,, State Geologist: of Kentucky; The Island Creek Oil Pool is situated in southwestern Owsley County, Kentucky, in the central western part of the Eastern. Kentucky coal field. It is fifteen milés im an-air line slightly west of south of Beattyville and twenty miles in an air line slightly west of south of the Big Sinking Oil Pool in Northern Lee County. Island Creek, thé principal line of local drainage, is an entrenched eastward flowing tributary of the South Fork of the Kentucky River. Physical relief varies from 50 to 450 feet in this district which, broadly described, is maturely dissected and steep of slope. The valley bottoms are entrenched and meandering, while the ridge tops are narrow and winding. The hard rocks exposed 2: the Surface and to) avdepthion *See Ky. Geol. Survey Series VI., Pamplet XII., 20, Illust., 55pp. 1927. FOURTEENTH ANNUAL MEETING 45 athletics and non-participants revealed only a negligible mentai difference and a five point difference in academic averages. The younger students were brighter and more successful, as a rule, than the older. Students from Kentucky high schools compared favorably with those from the East and from the North Centrai States and did better work than those from the South. The study suggests a close relation between intelligence and purpose in college with success within its halls. For a detailed account, sec ioum ot Applied: Psycholasy, Vol...12,. Noy, 5, pp.)517-23: 20. A Study of Moral Judgment. Paul L. Boynton, Psy- chology Department, Univ. ot Ky. The results of 10 tests are reported. Students in the Psycho- logy Department, 74 girls and 80 boys, took part. The papers were turned in unsigned. The following conclusions are drawn: Boys judge less strictly in moral situations such as those pre- sented in this test than do girls, the average score for boys be- ing 532.2, and for girls, 582.1. Girls show more intra-group dis- agreement than do boys, in practically all instances. It 1s worse for a girl to commit a given immoral or questionable act than fora boy to do it. A slight tendency appears for girls to be more uniform in their judgments than boys. Very great varia- tion in moral standards appeared. 21. The Social Origins of Religion. William H. Walker. Berea College. The theory that there is an impersonal stage of religion before the personal rests on a false interpretation of the religion of primitive peoples, like the Australians and the Todas. It assumes that primitive man develops from the impersonal to the personal, which is false psychologically. The reverse is the case, in spite of vague conceptions of the personal. The theory is due to the over-working of the definition of religion as “the effort to conserve socially recognized values.” That would make religion coextensive with all the activities of primitive man save actual eating and procreation. The differ- 46 THE KENTUCKY ACADEMY OF SCIENCE entiation of religion is in the agency thru which such conserya- tion is sought, namely, God or gods. But the idea of God deveiops for its own sake before 114 is used as a means to the conservation of other values. Man could not live in an unsocial world. Hence on the one side came the formation of social groups. On the other, came the socialization of the universe, the peopling of it with creations of his fancy, his need, with whom he could hold fellowship, to whom he could appeal for help. Only exceptionally has he ever been able to persuade himself that he is in an unrespon- sive world. 22. The Relation of Philosophy to Science. Glanville Ter- rell. Philosophy Department, Univ. of Ky. The line of argument was to show that scieuce deals with facts while philosophy is an exercise of pure reasoning {a priori) without the facts. Before the facts have been’ found) the question is said to be in the philosophical or metaphysical stage. As soon as the facts are reached the question at once passes over into the scientific stage. The speaker endeavored especially to show that the iruitfulness and econemy of ail scientific experimentation depend largely on the accuracy ana correctness of the thinking done while the question is stiil philosophical. Further, that the importance of facts, on which scientists are inclined to lay so much stress, is due largely to the interpretation of these facts by the human mind and the demonstration of the relation of these facts to the universe in which we live. This last is philosophy no less than the think- ing which precedes the facts and leads to their discovery. An isolated fact is without meaning. All great scientists have combined the philosophical attitude of mind, the ability to rea- son without the facts (a priori) and the scientific attitude, the ability to reason from the facts. The errors of one method. pure reason, must be corrected by the other, the evidence of the senses. FOURTEENTH ANNUAL MEETING 47 23. The Constitution of Living Matter. (Abstract.) Martin H. Fischer, Laboratory of Physiology, University of, Cincin- nati, Ohio. I. LYOPHILIC COLLOIDS. The characteristics of lyophilic colloid systems are best explained on the assumption that they are mutually soluble systems of the type phenol/water, butyric acid/water, etc. Any lyophilic colloid system (like soap/water or pro- tein/water) is, like phenol/water, capable of forming two types of solution, one of phenol in water and a second of water in phenoi. When an ordinary soap/water system is permitted to cool, say from 100° to room temperature, it changes from the first of these two types of solutions to the second. In the course of such change two zones of mixed systems are passed which ate; Of “Special significance. As ‘shown inthe “diagrams of Figure 1 we pass from the original non-colloid, “molecular” or ionized. solution’ of “soap: in water (zones A) thru: a first type of mixed system which is a dispersion of solvated soap in soaped-solvent (zones B, C. D. E) into a second which is a dis- persion of soaped-solvent in solvated soap (zones V. W. X. Y). At the bottom lies the second type of true solution which we have called water in soap (zones Z). All the systems between the true solution at the top and the true solution at the botto7i are, if the dimensions are correct, “colloid.” athis concept explains readily the “peculiarities’, of; the so-called lyophilic colloid systems. Obviously it sets no limi- tations upon the nature of the materials that: may makeup such a colloid system and makes no specifications as to the nature of the forces which guarantee its stability. They are any or all which may appear or be operative whenever “solu- tion” of any kind occurs. Electrical notions of colloid stability are at present particularly acceptable... But how can such be the dominant factors in those most typical lyophilic colloids which consist of nothing but nitrocellulose with ether and alcohol, agar-agar with water, or rubbed with benzene? They are of minor significance even in those lyophilic colicid systems 48 THE KENTUCKY ACADEMY OF SCIENCE which; are composed, of “an: “electrolyte. ‘and watem(ike soap/water). The most stabile of these systems show the least evidences of electrical charges. When such appear they are not the cause of the colloid behavior but the accidental con- sequence of having an overplus of “solvent” present in the system into which some of the soap has gone in true solution with secondary hydrolysis and electrolytic dissociation. SOAP IN WATER SOAP IN WATER a A ee byes ee 4 oe eS ore Ce 2 A B ee Bilin kee ee eee vernal @©@ ee, ee @@ Sa a a ae a a ee 2 Cc @e O| [Pe FF Fe ee ee @ @ eee yO RH HR YX @©G@00000003080 eK OF GOSS COCOCE8O® Bike Sk Se ie Se A SS a p||eeeeeoeeeoes | |e ae Be ee ee Se Se ee ae ©8008 OCO8O08 Nie Sk Se ie ke He Se Se SK ee EOE OEOEY Rae, ts ti, tain Atal E El IS ie | |U NN NAS F, v FIN W Ww X xX Y Y WATER IN SOAP WATER IN SOAP A Fig. ik B FOURTEENTH ANNUAL MEETING 49 This theory of the solvated colloid clarifies the concepts of hysteresis, gelation capacity, swelling and syneresis. Hyste- resis is the expression of the fact that solution takes time, wherefore two mutually soluble substances cannot quickly come to equilibrium. The point at which a lyophilic colloid “gels” (zones F) is that at which the solvated colloid phase becomes the external one. The system as a whole still carries at this point as an internal phase a solution of the colloid in the solvent. The combination marks the gelation capacity of a colloid with its solvent and is always greater than the solva- tion capacity. of the colloid. The latter is a measure only of the solubility of the solvent in the colloid material. The in- crease in the: volume of the latter as the solvent’ is taken, up measures its ability to “swell.” The zone Zin the diagrams covers the swelling capacity of a given material with its sol- vent; the gelation capacity embraces all the zones above this up to and including the zone V. As soon as this zone is pass- ed the external solvated colloid phase may not inclose all the solution of colloid in solvent, wherefore the system as a whole begins to sweat; in other words, exhibits the phenomenon known as syneresis. Colloid systems in which one of the mutually soluble materials is solid (diagram B) will obviously fail more easily to inclose adequately the internal phase than will such in which both materials are liquid (diagram A), wherefore colloids of the type sodium stearate/water, silicic acid/water, etc., show a greater liability to syneresis than more liquid ones like sodium oleate/water, rubber,/benzene, etc. II. CELLS AS COLLOID SYSTEMS. Living cells behave like hydrophilic (lyophilic) colloids. Not only are the laws which govern water absorption or secre- tion by the two identical but the absorption and secretion of dissolved substances are identicai in both. These analogies between living matter and lyophilic col- loid systems demand that the former find some place in the 50 THE KENTUCKY ACADEMY OF SCIENCE diagrams of Figure 1. The physico-chemical properties of pro- toplasm are such as place it definitely in the lower regions of the diagrams. Protoplasm is essentially, in other words, a solu- tion of water in protoplasm. ‘the more solid structures of the body never, normally, lie above the middle of the diagrams and even such liquid protoplasmic structures as blood and lymph cannot lie much above the level E. On the other hand, the more aqueous secretions from the body, like urine and swear, approximate the level A (that of the true solutions) tho even these, thru admixture with colloid substances (colloid salts and proteins)” are. better comparable to levels “hike. BeomiG a alice physical chemists have for the most part sought the solution of physiological behavior by trying to rediscover in living mat- ter the laws of the dilute solutions. But protoplasm does not lié in or near the levels A of the diagrams but nearest the levels Z. The physico-chemical laws which govern systems of this type are those which are most likely to find unobjectionabl: applicability to protoplasm. Ill... MUTUALLY SOLUBLE SYSTEMS AND PROTOPLASM. The system phenol/water in its two phases, water-dissolved in-phenol and phenol-dissolved-in-water, yields in handy labora- tory fashion the analogues respectively of the zones Z and 4 of the diagrams of Figure 1. Phenol is a crystalline material which, upon the addition of water, becomes an oily liquid. A chemist caugnt unawares is likely to-say that this is a concentrated (95 per cent)esolu: tion of phenol in water. But when more water is poured upos the oily mass two !uyers are formed. The first liquid was really a solution (about 5 per cent) of water in phenol and the new solution formed over the oily one is one of (about 5 per cent) phenol in water. If the thing is done quantitatively (50 cc. of melted phenol crystals being mixed with 50 cc. of distilled water) the picture represented diagrammatically in Tube 1 of Figure 2 is obtained. Above a lower, stratum (of 65 ‘ce, of hydrated phenol is found an upper one of (nearly) 35 cc. of FOURTEENTH ANNUAL MEETING D1 phenolated water. The lower one of these phases corresponds with the black zones Z of Figure 1, the upper one with the white zones A. Let tt be emphasized at once that the properties of living matter are rediscoverable in the lower phase (that of hy- drated phenol) and not im the upper one (of phenoiated water) as we have been taught. Proof for this may be brought as follows. 1. Living matter does not mix with water. A fowl, or a piece of muscle or a bone may be stewed or boiled indefinitety to make soup but it does not dissolve or mix with the water. Rained on, or in swimming, we do not dissolve in the surround- ing waters. Neither does hydrated phenol dissolve in or mix with the water phase that covers it. When Tube 1 of Figure 2 is shaken, the hydrated phenol breaks into droplets which float about in the water like so) many amebae. +2. [Pa 4 ‘ +Na0H ~ 2. The hydrophilic colloids derived from living plant or animal structures “swell” when thrown into water. Phenol, similarly, shows a 30 per cent volume increase when exposed to water. The basic hydration oj the protein colloids of the tis- sues may be enormously increased thru the additicn of any alkali jor. acid’ -Simularly, ‘alkalies: ancrease “the “‘swelling’. capacity of hydrated phenol as shown in the tubes marked 2 S74 and oot Picure: Z, 52 THE KENTUCKY ACADEMY OF SCIENCE 3. Living matter rarely “dissolves” any proffered substance as would an equal volume of water or any salt solution. Cer- tain things like the anesthetics and various dyes dissolve better in living substance than in water; others, like eosin or iodine. occupy a middle position; while various salts, particularly those of the heavier metals dissolve in protoplasm so poorly that some authors teach that they never enter the living cells. The same may be said, uot only in general, but almost specifically of the solubility characteristics of the hydrated pheno! phase as compared with the solubility characteristics for the same sub- stance of the phenolated water phase. 4. The electrical resistance of protoplasm is unexpected- ly high. In spite of the fact that living matter contains a fair fraction of electrolyte (say 1 per cent of salt in the ordinary soft tissue) it is nevertheless a very poor conductor of elec- tricity as compared with a solution of these salts in water. Where. with standard electrodes, a 1 per cent salt sclution will register a resistance of several ohms, the fluids (blood or lymph) or tissues. of the body wili register several hundred. The same is true when the two phases, phenol-dissolved-in-water and water-dissolved-in-phenol are compared. Even tho the for- mer contains only one twentieth as much “electrolyte” as the latter, the first shows a resistance of only 50 ohms, or less, compared with a resistance of 20,000 ohms shown by the second. The initially high electrical resistance of living matter 1s reduced thru “injury,” by acids, alkalies, various anesthetics and certain salts. The addition of the same materials to the hydrated phenol phase lowers its electrical resistance in the same striking fashion. IV. CELL “PERMEABILITY.” These findings are of significance for a better understand- ing of certain aspects of cell behavior, more particularly the phenomena of “permeability” cf “cell membranes” or of ”pro- toplasm”’ in general. The attempt is still being made to under- stand these phenomena thru some modification of Pfeffer and FOURTEENTH ANNUAL MEETING 53 de Vries’ osmotic concept of the living cell or Overton’s lipoid membrane modification of it. The physico-chemical and biologi- cal objections which may be raised against either of these notions are too numerous to reed repetition here. The living cell is capable of absorbing znd secreting water, of absorbing and secreting the most varied types of dissolved materials, the two moving at times in the same direction and at times in opposite directions. There can be no adequate physico-chemical! concept of the living cell which does not contain within it the possibility of understanding all these characteristics at one and the same time. The volume of the hydrated phenol phase described above “swells” and “shrinks” when subjected to the action of alkalies or Gf salts; it shows, in other words, the biological phenomena cf plasmoptysis and plasmolysis, just as does any hydrophilic colloid (protein) or the living cell. But such a phenol system shows also the “strange” phenomena of permeability to dis- solved substances so characteristic of living matter. It is quick- ly permeable, for example, to the most varied dyes; to another group of such or to iodine it is less permeable. While per- meable to the salts, hydrated phenol takes these up most slowly and in certain instances practically not at all. Identical observa- tions are characteristic of protcplasm and the living cell. The high electrical resistance characteristic of living mat- ter has always been difficult tc understand as long as we held to the view that protoplasm was essentially a somewhat modi- fied dilute solution. In spite of the conclusion that a physiologt- cal salt solution is supposed to be osmotically comparable with the salts dissolved in a living animal or its body fluids, the former will register only 1-5 to 1-35 the electrical resistance of the latter. This old biological truth can be understood oniy by denying to the salts found in protoplasm any large existence in uncombined form or by concluding that the cell is a dif- ferent sort of solvent for these salts than is water. Experimen, tal evidence supports both these conclusions. Aside from the fact that the electrolytes are for the most part “combined” with the protoplasmic constituents and are not “free’’ as in an ordi- 54 THE KENTUCKY ACADEMY OF SCIENCE nary salt solution, the high electrical resistance of protoplasm is further accounted for as soon as it is remembered that pro- toplasm is not a solution of protoplasmic material in water but one of water in protoplasmic material, a solution comparable, in other words, to the solution of water in phenol. The effects of acids, of alkalies, of single salts, of anesthetics, ete, all) of which reduce the normal electrical resistance of living matter, are then to be understood in the same terms in which these factors reduce the electrical resistance of systems of the type, hydrated phenol. Even the physiological antagonism between different salts so characteristic of living matter reappears in the case of hydrated phenol. V. REMARKS ON HYDRATION. These analogies between mutually soluble systems, hydro- philic colloids and living matter compel the conclusion that in the last named system we have to deal with what is essential- ly a solution of water in the pretoplasmic mass, a system which bears no relation in its fundamentals, therefore, to the ordi- nary dilute solution of our chemical laboratories and to the properties of which we have been so long accustomed to look for the explanation of physiological or biological behavior. If, now, we ask regarding the nature of such inverse type of solution, say of water in phenol, water in a coiloid or water in protoplasm, the answer, somewhat dogmatically expressed, is that in all these the water is no longer “free” but combined with the material which is hydrated. The combination is also quantitative in character, in other words, it is “chemical.” Can something be picked out of chemical laboratory experience to make clearer what is; meant? This can’ be done.) Whenia selid soap, like sodium stearate in water or alcohol, changes oa cooling to what even the chemists are willing to call a “solid solution” of the solvent in the soap, we have before us, in the end, something strongly reminiscent of the crystailization of any compound with several molecules of water of crystalliza- tion. When, on the other hard, some crystals of phenol take up a limited amount of water to yield an oily solution of water FOURTEENTH ANNUAL MEETING 55 in phenol, this is the analogue of what is seen when sulphur trioxide changes to the oily sulphuric acid upon the addition of water. In a certain sense, it all these illustrations, an anhy- dride becomes hydrated. It this general statement is correct, we should be able to find in homely laboratory materials the analogues of the prop- erties here emphasized for various colloid systems. The ordinary sulphuric acid of our laboratories is viscid and exhibits a Tyndall cone. These are properties familiar to us when discussing solvated coiloids. Upon exposure to an at- mosphere which contains water, concentrated sulphuric acid takes this up. The sulphuric acid, in other words, “swells.” Such swelling whether of sulphuric acid or of a colloid is asso- ciated with the liberation of heat. How, now, does the ordinary concentrated sulphuric acid of our laboratories behave electrically? As every chemist knows, not. as simply as. might be expected. The electrical resistance of dilute sulphuric acid decreases with every increase in the concentration of the acid. But this law.is valid-only for the heavily diluted acid. As soon as more concentrated sulphuric acid/water mixtures are approached, the electricai resistance increases with every increase in the concentration of the HzSOa, proof again that, with change in concentration, we pass from what was originally more of a solution of the sulphuric acid in the water to one of water in the sulphuric acid. VI. REMARKS. The significance of the point of view which is here being urged can be demonstrated by some simple jaboratory experi- ments. Other differences besides those already discussed shou!d be discoverable between the two types of solutions, A dissolv- ed in Band B dissolved in, A> To two of these which’ are of great significance for our every day biological thinking, I want to refer now. The first has to do with the behavior of indica- tors and the second with the type of equilibrium establishable 56 THE KENTUCKY ACADEMY OF SCIENCE if in consequence of the fact that a chemical reaction is allowed to take place in the one or the other of these two types of solu- tion. 1. Experiments with Indicators. Suppose we ask what is the reaction of any chemically neutral soap like a mixture of 20 per cent potassium oleate with water. Such a mixture is viscid and reminiscent of blood plasma or egg white. To test the neutrality, we drop into the liquid a little phenolphthalein solution. The mixture remains colorless. Water is now carefully poured down the side of the tube until it is filled. The tube contents become increasingly re: as the dilution becomes greater. At the bottom, the soap/water mixture is still colorless, but at the ‘top it is: bright red.” Be= tween the bottom of the tube and the top any pH that suits us may be read off. The physica! chemists tell us that we have in this instance begun with a “concentrated” solution of an electrolyte in water—a solution in which ionization was “sup- pressed’—and that thru dilution with water, increasing hydro- lvsis was invited. Potassium hydroxide being a stronger alkah than oleic acid is an acid, an overplus of hydroxyl ions yielded the red color with phenolphthalein. I do not deny that some of these things do happen but the first and primary change in this experiment has been missed. This is the conversion thru dilu- tion of what was originally a solution of the water m the soap to one of the soap in the water. The indicator serves to show us that these two solutions are different. What is of importance to us is the fact that we have been using indicator methods derived from and perhaps applicable to the study of solutions eof the type, electrolyte dissolved in water, upon solutions of the opposite type (blood, lymph, body tissues) as tho these were solutions of the same construction. Obviously there is danger in such thinking. In the case of potassium oleate with water, the solution of water in soap passes smoothly and quickly into the solution of soap in water. What is here discussed can, therefore, be even more strikingly illustrated if, instead of a liquid soap/water FOURTEENTH ANNUAL MEETING -j ol system, a hydrated solid soap/water system is chosen. Any cake of toilet soap which contains a considerable fraction of water may be used, tho the experiment can be made more scientific and expensive by utilizing a chemically neutral solid white soap of the acetic series. If some phenolphthalein is pour- ed over the surface of such a cake, no color change takes place. As soon, however, as distilled water is sprayed upon it, the cake drips red. Here is the fundamental answer, I think, of how a neutral pancreas, salivary gland or kidney comes to yield an alkaline secretion. An acid “secretion” may be derived from a neutral source when, instead of a soap, a chemically neutral acid proteinate is diluted with water. Such indicator experiments may be repeated upon the con- centrated sulphuric acid which has just been declared to be 2 hydrated system not dissimilar to the hydrated colioids. Methyl red turns red in acids and yellow in alkalies. When this indica- tor is added to concentrated (Sp. Gr. 1.84) sulphuric acid, it turns bright yellow. Sulphuric acid is, therefore, violently ai- kaline. Distilled water is now added. With a little, the sul- phuric acid, to judge by the indicator, turns neutral, and with more, the mixture becomes increasingly acid. In the fact of such facts, what must be thought of the present-day unrestricted application of indicator methods to even the ordinary chemical systems of the “concentrated” type found in our laboratories, or specifically to the biological systems upon which the physiolo- gist or biologist works daily? Zo) Syuthests in Living Matter. The evidence indicates, therefore, that neither living mat- ter nor any fraction of it is to be thought of primarily as a dilute solution or as anything approximating such a system. It is, rather, a protein to which the salts have been bound chemi- cally (fundamentally as a base-protein-acid compound) and in which the water has then been “dissolved” (or to which the water has been bound as a hydrate). This triple affair is to my mind the fundamental unit of the living mass. 58 THE KENTUCKY ACADEMY OF SCIENCE If this conclusion is accepted, then the chemical reactions which occur in living matter must occur in a medium far different from ordinary water. But this coinpels the conclusion that hving matter is normally a practically anhydrous medium. The view brings significant corollaries with it. Jt means that the chemi- cal reactions characteristic of the normal life of the cell occur in an anhydrous medium and that their course and products must, m consequence, be quite different from the course and products of these same reactions occurring and familiar to us in aqueous solution. The physiologists and the biochemists are always astonish- ed at living matter’s remarkable powers of chemical synthesis. While the chemist, by the use of acids and alkalies, or heat and water, or thru the gentler action of those fragments of the living mass which he calls ferments has been able to break up the complex organic proteins, carbohydrates and fats into their simpler building blocks, he has had great difficulty in resynthesizing these materials into their original forms. Yet living matter does this with the ‘sreatest ease. A beeisteak with bread and butter which melts in the lumen of the intes- tine into amino-acids, simple sugars and fatty acids and glycer- ine and is thus “absorbed,” is so rapidly resynthesized into protein, glycogen and fat by the living celis that the building blocks can scarcely be discovered in the blood or lymph which carries the absorbed meal away. It was a great step for- ward when A. Croft Hill, Kastle and Loeverhart, Hanriot and their successors first showed that the ferments were capable of catalyzing not only an analysis but a synthesis. It must be said, however, that in test tube experiments this “rever- sible action of the ferments’ never proved to be very great so far as the synthesis half of the problem. was concerned. Still, in the body, synthesis is as easy as analysis. The answer to the problem is, I believe, written in the fact that nature always makes her analyses in an aqueous medium and her syntheses in an anhydrous one. The agencies which digest a meal in the alimentary tract always work in the presence of much “free” water; the same agencies working in the body substance operate in the presence of none. FOURTEENTH ANNUAL MEETING 59 The importance of this arrangement is illustrated in the making of soap. To make soap, a fatty acid and a suitable quantity of an alkali are put together with very little water. As chemical combination takes place, the soap as formed binds the; water so -that-in the end- 100 per:cent of hydrated soap ‘is formed—synthesis, in other words, is carried te completion. When water is added to the reaction mixture, the soap hy- drolyzes into alkali and free fatty. acid—a reaction which again, at proper concentration, tends to be complete. With a medium amount of water either reaction “tends toward an equilibrium’’—in other words, to a mixture of soap with alkali and fatty acid. Iron and iodine combine directly to iron iodide. If this reaction is carried out in water, nothing but a reddish green mixture of a little iron iodide, much iron hydroxide and hydriodic acid is obtained. Add cane sugar to the original mixture (which combines with the water to make “syrup’’) and a clear solution of ferric icdide in the hydrated sugar 1s. obtained,—in other words, with water, hydrolysis and analysis, without it, synthesis. Over what route, in general, does the organic chemist accomplish his syntheses? The fact has -o be whispered to the pure line physical chemists—the anhydrous one. Fat pro- duction in the body is ester production. What is the approv- ed method for producing an ester? When, to illustrate the general truth, ethyl butyrate is diluted with a considerable quantity of water, the ethyl butyrate collar becomes percepti- bly thinner. because it hydrolyzes rather rapidly into ethy! alcohol and butyric acid both of which are readily soluble is water. This hydrolysis may be hastened by adding sulphuric acid. We deal here with the decomposition of an ester in the presence of much water (the analogue of the digestion of a “fat” into fatty acid and alcohol in the lumen of the gut). In the reverse of this experiment butyric acid has added to it a molar equivalent of absolute ethyl alcohol (17.6 gms. butyric acid + 9.2 gms. ethyl alcohol). If the mixture is merely 60 KENTUCKY ACADEMY OF SCIENCE allowed to stand, a considerable synthesis of ethyl butyrate takes place within a few days. To hasten the matter, the con- tents of the tube are divided between two tubes. While one is kept as control, the second has added to it a few drops of concentrated sulphuric acid. The contents of both tubes are now diluted with much water. The original mixture mixes with the water to yield a water-white fluid but the mixture treated with sulphuric acid has a thick collar of ethyl butyrate at the top. In place of the butyric acid any other water-soluble fatty acid (thru valeric) may be employed and in place of ethy! alcohol and other water-soluble alcohol (thru butyl) while instead of sulphuric acid any one of several other “driers,” like phosphorus pentoxide, calcium chloride or any coiloid capabie of maintaining its water-holding powers in the mixture may be used. The essential thing is the removal of the “free” water present in the original mixture or formed chemically. VI. CLOSE. Where must the new physiologist or biochemist look to get answer to his eternal question regarding the nature and the constitution of living matter? He can obviously not get it thru further increase in his knowledge of what l:ving mat- ter yields upon chemical analysis. And his iiving mass :s evidently not, as so long taught, a dilute solution of these materials in water. Wherefore it seems that he must forsake increasingly the methods, findings and modes of thought of the dilute: solution chemists: Living matter is a* solution or the “concentrated” type; to. use, the’ words /ot “they physical chemist, and of the solvated, water-dissolved-in-x type to use the terminology of the colloid chemist. It :s the properties of such systems which will interest the physiologist of the future. (1) The same is true of nitration and sulphonation. In nitration the sulphuric acid of the nitric-sulphuric acid mixture used does not appear in the end products but serves to keep the reaction mixture anhydrous, while in sulphonation the excess of sulphuric acid used serves the same end. FOURTEENTH ANNUAL MEETING 61 We are only on the threshold of an understanding of their properties, laws and behaviors but as such 1s obtained we shail discover as corollary an understanding of the nature of hving matter itself. 62 THE KENTUCKY ACADEMY OF SCIENCE MINUTES OF THE FIFTEENTH ANNUAL Masai, MAY" 1 Zire 1928 The meeting was called to order at 9 o’clock, a. m., by Presi- dent Valleau in room 200 of the Physics Building, University of Kentucky. About 14 persons were present at the beginning, others coming in later. The Secretary's: report was tread in’ outline by Drymeten Lhe Treasurer's report was read by Prot: Andersonsaiive President appointed as auditing committee, Messrs. Jewett, Marshall and Fergus. Dr. Peter reported that the council had’ held: one niecting for routine. business and approving accounts. The election of the 10 members was by letter vote. Dr. Peter submitted volume 2 of the Transactions as the report of the publications committee. Report) of, the ‘membership: committee, was) read™ by. (Dir Koppius, submitting 16 names, which included the 10 men- tioned above. Resolved that the Secretary cast one ballot for all members and they were elected unanimously. The President appointed Messrs. Bangson, McHargue and Payne as nominating committee. The President delivered his address on tobacco disease in- vestigations in Kentucky. The general meeting then rose, and the divisions separated. The general séssion was called to order by “resident Valleau at 2 o'clock. The report of the auditing committee showing that the Treasurer’s accounts had beer examined and found correct was adopted unanimously. FIFTEENTH ANNUAL MEETING 63 Dr. Koppius, for the Membership Committee, presented a supplementary report recommending the following persons for membership: CG |. Latimer, Dept. of Mathematics, University of Kentucky. Ro Rush, Head: Department of Chemistry, Centre College; Danville, Ky. George B. Wurtz, Weather Bureau, Lexington,’ Ky. ieeG. Wall, Centre ‘Collese, Danville, Icy: RetC.-Miller, Animal Husb..Dept., Experiment : Station, » Lex- ington. HG. Campbell, Ph. D., Dean’ of Men and Head of ‘Biology Dept. Transylvania College. William M. Clay, Instructor, Biology Dept., Transylvania College, Lexington. J. L. Leggett, Professor of Psychology: and Education, Tran- sylvania College, Lexington. Frank M. Shipman, Asst. Prof. Chemistry, Univ of Louisville, ‘ Louisville. Grover ll. Corley, Ph. Dy Asst. Prot: ‘Chemisiry, Univ. of kous- : ville, Louisville. ice Capps... Dept..of Chemistry, (Berea. -Collece, Berea; ‘Ky. The report was adopted and these persons were unani- mously elected members of the Academy. The Nominating Committee reported nominations for officers as follows: for. President:< G. Davis: Buckner: Hor vy ice resident _ George DD Smith. For Secretary: A. M. Peter. or, Preasurer >. W. 5. Anderson: For Member of the Publications Committee: W. R. Jillson. Mon svepresentative.in the Council of the -A.7 Aw AO Sie Aca: Middleton. The report was adopted and, upon motion, duly seconded and carried’ unanimously, the Secretary was: ordered to ‘cast one ballot for all the nominees. This having been done, these 64 THE KENTUCKY ACADEMY OF SCIENCE persons were declared unanimously elected to their respec- tive offices. Professor Roberts reported briefly on the First Interna- tional Soil Congress. The following resolution was adopted unanimously: Whereas The Fifteenth International Geological Congress will meet in Pretoria, South Africa, next May. Resolved that Dr.) Jillsonv represent the Aczdemy -at- this “Congress! President Valleau gave a short report of the Publications Committee stating that it was their opinion that the Transac- tions should be published each year but the material included be cut down so that it comes within the income of the Academy. Dr. Stakman then delivered a very interesting illustrated lecture on “Biologic Specialization” followed by a general dis- cussion in which several members participated. There being no further business, the Academy adjourned sine die. Ai MsePE TEAR, Secretary. REPORT Or THE SECRETARY: FOR 192728 The President appointed the following Membership Com- mittee: O. T. Koppius, Chairman; S. M. Mayfield, and) MigA® Caldwell. The following 4 persons who were elected at the last meet- ing have qualified and been added to the roll: Prof. James ;L. Graham, University of Kentucky. Mrs. Charles F. Norton, Transylvania College. Miss Hilda Threlkeld, Hamilton College. Prot, /G.\C, Bassett, University of Kentucky. FIFTEENTH ANNUAL MEETING 65 The following 10 persons elected by the council have quali- fied; Dr. Malcolm Y. Marshall, Henderson, Ky. Prof. Gordon Wilson, Bowling Green, Ky. Pros He Wilbur ,Cook, Centre Collese, Danville, Ky. Mr. William Marshall Bullitt, 1711-26 Inter-Southern Bldg. Louisville, Ky. Prot. Charles Hire, State Normal.School, Murray, Ky. Mr. Nat L. Shepard, c/o Franklin Fluorspar Co., Marion, Ky. Mri W. Taylor, Poultry. Dept... Experiment Station, Lex- ington. Prof. M. E. Ligon, University of Kentucky, Lexitigton. Mr. Donald James Munroe, 374 Spring St., Lexington, Ky. Prom Birkhead, ‘Supt: City Schools; Winchester, Ky. Nine persons have been dropt from the rol! for various reasons: Stuart -Weller,: Jean McKinnon; Homer Cooper, R EePonter, W. |. Craic, |. [: looper, Gladstone Kofiman,: Karl Waugh and W. S. Webb. Dr. Lester has resigned to take effect at the end of the fiscal year, he having left che state. The total membership is now 179, including 90 national and 54 local members, making 144 active members, besides 22 corresponding members and 15 honorary members. The membership may he classified as follows: Active members in good standing, including two life UME NID ID ET Syl le helen MN to es Dre ihe Peeall Bee eer 114 INGtiV en IMenLb ers win arreabs: desyearia stam ene. |e ie 10 INCtIVe MeMpDEnSeIl satrearsy 24 yeatsy. 0s ude eS 20 Correspondinic: wmreminens tee tet vite Teens uh eo 22 Elonoreryaimemibersusl ss ea Aili) 8 a eG ene Cine Se 13 66 THE KENTUCKY ACADEMY OF SCIENCE Number of members at time of last meeting (1927) 20. 174 Dropped! tor alllgreasons. ee. ee % 165 NewrdMembers vadded 2:20 yan a 14 ‘Total 223s aaa eee 179 In January of last year President Burroughs appointed the following committee to attend the First International Con- egress of Soil Science at Washington, in “June: 19272 {Georce Robests,, Chairman; P.“E. Karraker and’ Dr. J-"S., Weblarene These men attended the meeting. At the request of Shirley W. Allen, Forester, The. Ameri- can Forestry Association, the Secretary wrote to our congress- men in Washington (Dec. 31/27) urging the passage of the McNary-Woodruff Bill (S. 1181) which was to come up very shortly thereafter for hearing. These letters were duly ac- knowledged. The Secretary received a réport from the Kentucky asec tion of the Mathematical Association of America, by Arthur R. Fehn, of Centre College, Danville, Ky., August 20, 1927. with regard. to their formine a separate. division in athe Academy. The letter follows: wives dear r--Peter: I have been instructed to inform you that the Ken- tucky Section of the Mathematical Association of America does not favor becoming affiliated and holding joint ses- sions with the Kentucky Academy of Science. However, it is the opinion of the Association that mathematics should be recognized by the Kentucky Academy of Science and therefore it recommends to the Academy of Science that the name “Physical Section” be changed) to readethe “Mathematical and’ Physical: Section’ of they Kentuck,, FIFTHENTH ANNUAL MEETING 67 Academy of Science. This action’ was. taken-—at the meet- ing May 14, 1927, of the Kentucky Section of the Math Assoc. of America. Sincerely yours, Gisned) AR TAUIR Re MEEDN O Sec y=dineas: iy Seco1 WE Ae rot pe Volume II. of the Transactions has been received from the ‘printer and distributed to all members not in arrears for dues. Respectfuliv submitted, AS VE PB AMER: Secretary. TREASURERS REPORT Palanceutny pane Wha ye4. O27 cee aaa ical eee $ 369.62 Receipts: from. May 4.1927: to May 10; 1928 468.72 Otay Sets Ree ees ee $ 838.34 Motal.expenditures from May 4, 1927 to May 10,1928... 574.83 Balancesin batikest: 23S" 263,51 Investments of Life Membership funds: Pexineton Building Woen Stock 2 “ac $ 51.00 Was: Postal Savings Certincate 5. 25.00 $ 76.00 WW. St ANDERSON, Treasurer 68 THE KENTUCKY ACADEMY OF SCIENCE PAPERS READ‘ AT THE 15TH ANNUAL MEERTING: MAY WZ, 1928. 1. Tobacco Disease Investigations at the Kentucky Agri- cultural Experiment Station. W. D. Valleau. President’s ad- dress, (Abstract.) Among the most important diseases of tobacco studied by the Agronomy Department in the past 8 years are black root- rot of Burley tobacco, the so-called brewn root-rot, the virus diseases, of which mosaic is the well-known representa- tive, the leaf-spot disease; known variously as wildfire, rust, blackfire, fieldfire and angular leaf-spot, thought to be caus- ed by at least three distinct species of bacteria, and a physic- logical disease called frenchine. Black Root-Rot. Caused by a soil-inhabiting fungus of very wide distribution (Thielaviopsis basicola (Berk) Ferraris) causes most damage in the Burley district, probably because of a difference in soil reaction, inasmuch as neutral or slight- ly acid reaction seems to favor the disease.* Black root-rot causes serious loss in about a third of the crops in the Burley district. Tests at Lexington indicate that the common varie- ties of dark tobacco and Burley are very susceptible, except certain moderately resistant. strains of Burley. Extensive breeding work has been done to develop resistant strains of both Burley and dark tobacco and the moderately resistant burley has been used quite largely, with success. Brown Root-Rot. Appears to. be present in all tobacco- growing areas of Kentucky, tho not recognized by growers. It causes rotting of the new rootlets as they develop, follow- ing setting and apparently during the rest of the season. The disease appears not to persist in the soil, and growing tobacco year after year on the same land seems not to have a cumula- tive effect, but rather the opposite. Control probably is to be expected thru cultural methods. *Mass, Agr. Expt. Sta. Bull. 299. 1926. FIFTEENTH ANNUAL MEETING 69 Leaf-Spot Diseases. These are of great economic import- ance especially during wet seasons. Two bacterial diseases, angular leaf-spot and wild-fire are concerned but their im- portance has probably been vover emphasized as factors in spotting of nearly mature tobacco. There appears to be a physiological disease occurring toward maturity and during wet periods which has been contused with the bacterial diseases. This type of spot is characterized by concentric zones. It has been produced in the greenhcuse in the absence ot the leaf- spot bacteria and cases have been observed in the field where complete control has been obtained thru the use of sufficient quantities of well rotted manure. Sanitary measures have been found insufficient to control the bacterial diseases in the plant bed and the suggestion is made that weeds may act as over- wintering hosts of angular leaf-spot and wild fire. ve Mosaic. Caused by a “filterable virus” and present almost everywhere that tobacco is grown. At least four distinct strains, were observed. It is an important disease to many Kentucky growers, tho some suffer little loss from it. Ap- parently, infection occurred during transplanting, the plants in the bed being free from the disease. The possibility of infec- tion from the hands of the workers suggested itself, inasmuch as they habitually chewed natural leaf tobacco. Experiments showed that when these men kept their hands clean and chew- ed only sterile tobacco while pulling and setting platts, infection ithe held averaged less than 0.5 “per cent, against about 3 per cent ordinarily. Also, if the hands of the workers were dipped in a decoction of natural leaf tobacco, before pulling plants, infection ranged as high as 80 per cent. The simple precaution of having the men who pull and set the plants. chew only sterile tobacco, avoids infection almost entirely. Tests of old samples showed that the disease may persist m the dried tobacco leaf for 31 years. Tests of commercial tobacco showed that most of the granulated smoking tobacco. cigarettes and five commonly-used plug tobaccos were some- what viruliferous. Most of the plug tobaccos, however, and 10"or 12 of the: twists: tested, were free from. the disease. 70 THE KENTUCKY ACADEMY OF SCIENCE Other Virus Diseases. Several diseases were studied, the viruses of which do not survive in cured tobacco but over- winter on perennial hosts. We have named these Ringspot, Putt, Vein-banding, Etch; Etch —:, Severe Etch; and- Coarse (Bich They are injurious to other p!ants than tobacco, such as toma- toes. Probably some or all were derived from potatoes. Frenching. ‘True frenching is quite commmon in Kentucky but is not serious except in spots in an occasional field and then, perhaps, only in certain seasons. It is characterized by the top of the plant becoming quite chlorotic, sometimes near- ly white. Our experiments, using a forest soil and sand cul- ture, show that the disease is caused by lack of avaiiable nitro- gen in the growing point of an otherwise healthy, rapidly- growing plant. In this soil the remedy is application of nitro- genous fertilizer, as needed. This work on trenching may lead to the understanding of certai: diseases of other plants. 2. Recent Developments in Investigations of Vitamin Bb. Miss Statie Erikson. Home Economics Department, Univ. of Ky. A preliminary report of work in progress. 3. Mineral Metabolism During Pregnancy. ODaniei J. Healy and Floyd E. Hull. Ky. Agricutural Experiment Station. In-aistudy made at the Experiment Station, the authors obtained the following resuits: Average inorganic blood- calcium content of 8.normal: sheep, in July; 13:1, me peralooice Of ‘serlim; ‘of ‘S.normal, pregnant ewes, in December.9 sums, of 7 normal pregnant ewes, at term, 9.1 mg; of 13 ewes having acidosis of pregnancy, 6.6 mg. The average content of phos- phorus and of potassium for the same normal ewes, at term. was 5.1 mg and 22.4 mg, respectively, per 100 cc of serum, and for the ewes with acidosis, 5.6 mg and 43.0 mg, respectively. The average ratios of calcium to phosphorus to potassium were 1 : 0.56 : 2.47 for the normal ewes at term, and 1 : 0.86 : 6.5 for those with acidosis. With diminished buffer value in acido- sis of pregant ewes, there are unbalanced calcium, phosphorus and) potassium ratios. For the full’ report, see Jour Aim aver Med). Asso, 72) (1928) p. 511, “and ‘Cornell’ Veterinarian 1026, Dio: FIFTEENTH ANNUAL MEETING 71 4. Hemoglobin Determinations. J. S. McHargue and Daniel J. Healy: Ky. Agricultural Experiment Station. The von Fleischl, Dare, Tallquist and Newcomer methods were compared using 18 sampies of blood from as many dif- ferent white rats. The average readings, as per cent, were: von Hleischl,,86.7;, Dare, by. McHareue, 78.7, by Healy, 87.1; Tali- quist, 76.5; Newcomer, 90.4. Agreement of readings by the two observers was good, except with the Dare method. The authors prefer the von Fleisch] method for clinical use because of its rapidity, the small quantity of blood required, the close agreement between different observers and the agreement of the readings with the condition of the animals. 5. The Production of CO» From Irreversibly Injured Cells of Nitella Fiexilis. P. A. Davies, Laboratory of Plant Physiology, University of Louisville, It is a well established principle that COz2 is produced ab- normally in stimulated and injured tissue. The mechanism of COz production by “dead tissue” is in doubt. “Irreversible in- jury’ and “death” are not synonymous terms, for. irreversible injury may be considered to occur at that point on a curve of injury beyond which the cells fail to recover beyond a certain point, while “death” signifies inability to recover to any degree. Whexauthors experiments’ seem. to indicate that the rate of COz production from cells of Nitella flexils drops below the normai rate at the time of, or-very shortly after, irreversible injury occurs= The findings are not in‘agreement with those of other workers, who found an increased rate (above the normal) of COz production with other types of tissue for an extended period after the cells were dead. For complete data, see Bot. Gaz. 87; 660-664, 1929. 6. Canebrakes of Kentucky in Prehistoric Time and the Importance of Cane as a Forage Plant. J. S. McHargue, E. S. fill and W. A. Anderson, Jr.,.Kentucky Agricultural Expert- ment Station. Analyses of 2 samples of native cane are reported. See p. 72. THE KENTUCKY ACADEMY OF SCIENCE 72 ‘SOSSBIS OSBIOJ UOWWOD oY} JO Jey) Spenbe saavey, ay} jo JUaJUOD ULejJoId ONY, D6 i F0 7°" oom 88B" sy CoE ‘OU 006° G0¢ OZS8'T 669° $éT° PST’ 200° 690° L¥0° 990° 9E8°C OFS’ 9LL°9 OLG'9 STBYS sunod SY[BIS woody sunoyx sqyjyeeys GPS” OSL'T OPP E 008'T 060°C G¢c'Pl 883° 696° 086% ‘0 U 680° L8T° P90" 8h’ OLS P96" OOT'T OGL T 610° VIL’ 0&6" “AY T80° GtT’ “IY 980° LEG T00° T00° T00° 100° SCO" 660° LTL 896°E G9T*8 9PFG°C 060°9 08'OT S¥[VI1S soypouRsg g ‘dues SoABaT (BJ) 3x90 10NI1a Gey ee (4g°9XN) Uloj}olg OG eGpe sr ates amie ae N ‘Uoes01jIN ORG re ewer or srenera ears Sa caregenaS S ‘anypusg Pei[ton Skis eke Seer era eS BN ‘uLNTpos CONC 0) i) Beeaitbeteiete tease Asa ‘“M ‘uInIsse10g UQ:Gcee > ease ss ee d ‘shaoydsoyd OeRSY LS assess is eee es ZN ‘UINTSOUSeIN 0G procs sess ee BO ‘UINpTeg Oi Qian ee ae es UW ‘asSouURsUBI Gib Olin oo aera oes ees eee OW ‘UOdT G8 Auris Aoseseru sel gee ase “OIS ‘BOILIS DIGS ORS nee: | aces Ramey seceneres UsV ‘dures Ssoaray :[BLI0}VUL 9dAJ-91NYSIOW 217} jo juoo 10d se sask[Bpuy FIFTEENTH ANNUAL MEETING -J or) A historical review is giver: to show the abundance of cane in Kentucky in pioneer times and that certain wild animais subsisted on it. Also that it was found to be an excellent forage for livestock, both summer and winter. 7. Experiments in Methods of Inoculating Soybeans. P. E. Karraker, Ky. Agricultural Experiment Station. Results were reported from field experiments planned to determine the relative effectiveness of applying inoculating soil in different ways. Various amounts of soil were applied broad- cast, mixed with the seeds in the hopper of the drill, and a¢- hering to moistened seed. Small amounts of soil applied direct- ly with the seed in either of the latter two ways gave better production of nodules than the broadcast application. Equal production of nodules was secured from half as much soil ad- hering to the seed as when mixed with the seed in the hopper of the drill. Soil gotten three years after soybeans had been grown on the land gave good production of nodules, but that gotten within one year was somewhat more effective. Soil taken from around the roots of soybeans grown in rows was appreciably more effective than that taken from midway be- tween the rows. Soil after retaining in air dry condition three months was slightly !ess effective than that kept moist. Inoculation increased the weight of hay one year but not the other. The percentage of nitrogen in the hay was marked- ly increased both years. 8. Chicken Feed and Mold. L. V. Amburgey, Department of Feed Control, Ky. Agricultural Experiment Station. During the starting and growing period ot chicks in the spring, the Department of Feed Control receives numerous samples of starting and growing mashes. The feeds usually are accompanied with the statement that the chicks are dying and that the feeds are suspected of poisoning them. Frequent- ly it is stated that the feed is moldy. 74 THE KENTUCKY ACADEMY OF SCIENCE In March, 1928, two such samples were received from differ- ent sections of the same county; one sender complained that baby chicks were dying in “piles”; the other, that pullets were dying at the rate of 40 a day. A microscopic and chemical anaiy- sis of these feeds failed to reveal the cause of the birds’ death. unless it was due to molds which were present in each feed. With the object of determining whether or not these molds were responsible for the death of the chicks, the following ex- ce periments were carried out. A sterile raisin agar medium was prepared and inoculated with very small amounts of feeds. At the end of twenty-four hours, molds had developed enough to be visible to the naked eye. In seventy-two hours f:iting bodies had developed and an attempt was made at classification. Numerous molds were present and the one that was most conspicuous was isolated and classified as Fusarium. Two portions, of about 100 grams each, of a different feed were taken, one of which was inoculat- ed with the isolated mold, the other with all the molds. These feeds were then placed in an incubator at room temperature and. leit there for’ one week. During this time, molds «had developed on both feeds to such an extent that there appear- ed to be more molds than feed. Enough fresh feed was thoroly mixed with each of the two 100 gram portions of moldy feed to feed*two pens, No! Il and No. 2; of two pullets each fora period of ten days. Pen No. 3 of one pullet was used as a check. The following results were obtained: Pen No. 1 fed isolated mold (Fusarium) with xo ill efect. Pen No. 2 fed all the molds with no ill effect. Ii. order to test the possibility that the original feed did not sicken the pullets, this feed was fed to the check pullet with no ill effect. Therefore, we formed the following con- clusions: 1. Moldy feed does not ordinarily cause the death of chickens. Z,. The feed in the first place cid not cause the chickens’ death: This investigation will be continued as circumstances permit. FIFTEENTH ANNUAL MEETING =~] vi 9. Oxidation of Sulfur in Limed and Unlimed Soils. O. M. Shedd. Ky. Agricultural Experiment Station. This investigation consisted of a study of the oxidation Mor sulfur, both“in the presence and absence of calcium ‘carbo- nate, when added to 31 Kentucky surface soils. The treated soils containing 15 per cent of water were incubated at rooim temperature for 4 months, with occasional stirring. Suliste sulfur, hydrogen ion contentration, acidity and alkalinity de: terminations were made. Sufficient quantities of sulfur were oxidized in every soil, with or without calcium carbonate, eve? after 30 days, to supply the sulfur requirements of almost any crop under maximum production. Very little consistent reia tion was found between the hydrogen ion concentrations of “ne treated soils, either in the initial or final pH values, and their acidity or alkalinity by nitration or the amounts of sulphur ox1- dized by them. For a detailed account of the experiments, :¢e Soil: Science; 26 (2), pp: 93-105. 10. Causes Affecting the Apparent Geographical Distribu- tion of Cancer Mortality in Kentucky. W. G. Burroughs, Berea College: According to statistics of the State Board of Health, the death rate from cancer, per 100,000 for 1911-1921, in the princi- pal physiographic divisions of the state was: Bluegrass, 62.4; Southern and Southwestern carboniferous Plateau, 47.9; Knebs 40.8; Jackson Purchase, 37.8; Western Coal Field, 36.4; Moun- tains, 20:9)- The death’ rate is decidedly greater’ in.cities thas in rural districts, perhaps because of the presence of hospitals in the cities, and because of better diagnosis. This circum- stance may account for part of the difference in different areas. but other causes must operate. Differences in the character of the food should be studied, especially with reference to possible causes of irritation. 11, ‘Gravitation. “Daniel. |; Healy, Ky. Agricultural Ex- periment Station. The swinging of a chemical balance with 100 ¢. in each pan, was timed, at about 8 A. M. and 5 P. M. The numbe: of 76 THE KENTUCKY ACADEMY OF SCIBNCE seconds required for the deviation of the pointer of the swing- ing balance to change from 10 to 5 on the scale was observed. The average of many observations showed an appreciably longer time in the afternoon than in the morning. On certain days, the time was much longer than the average. The author argues that variation in the force of gravitation is indicated 12. Air Adsorption in Water Vessels. Charies Hire, Mur- tray State Teachers College. Everyone has observed the accumulation of bubbles of gas on the bottom and sides of a vessel in which water is being heated. It is commonly believed and sometimes stated in science texts and by science teachers that these bubbles consist of air which has been driven from the water as its temperature rises. Knowing, as we do, that water readily ab- sorbs air and many other gases under ordinary conditions of temperature and pressure, this statement is believed without difficulty unless one asks why the air bubbles should go +o the bottom and sides of the vessel as they form rather than the top. A study of this question and the fact that some vessels. for instance everwear aluminum, collect more bubbles tha: others, lead to the experiments described in the followire paragraphs. Two pyrex beakers of 600 ccm, capacity were washed in warm soap water, rinsed with tap water and wiped dry with a towel which had been slightly used in the laboratory. The beakers were then partially filled with tap water and heated. Bubbles formed in the ordinary then emptied, one washed, rinsed and dried again, both pas- tially filled with tap water and heated. The beaker which had been washed the second time accumulated practically the same amount of gas bubbles as before, while the one which had manner. Both beakers were not been washed the second time accumulated almost nore Both beakers were again emptied and allowed to stand in the open air for about an hour after which they were refilled with tap water and heated. Both accumulated practically the same amount of gas as in the first ‘test. Both beakers were agam FIFTEENTH ANNUAL MEETING 77 emptied, washed in : warm chromic acid cleaning solut.on which removed all traces of organic matter, rinsed with tap water, refilled, and heated, and practically no bubbles formed on either. However, when the beakers were washed with the warm chromic acid, rinsed, and wiped with the laboratory towel which probably was not entirely free from fatty and other animal matter, both accumulated bubbles very readily. Brief- ly, it was found that either or both beakers could be prevent- ed from accumulating the gas bubbles by cleansing thoroly with warm chromic acid, rinsing, refilling and heating quickly However, bubbles formed if several moments were allowed to elapse, with the beakers either empty or full, between the rins- ing and heating. These tests were repeated with distilled water with exactly the same results. Several variations of the tests were tried, but an example from one other series will suffice for this paper. The beakcrs were cleaned with chromic acid, rinsed, and dried with heat. c<\ cross of organic material was then marked on the bottom @tone beaker by. means ‘of the experimenter’s- index finger which he had rubbed across his face. Both beakers were then partially filled with distilled water and the bunsen applied As the temperature rose, the cross became clearly visible as two intersecting lines of bubbles. This was used as the “in- dex cross.” Some redistilled water was boiled in a chemicatly clean vessel for several moments to remove all! absorbed gases then quickly cooled to room temperature by setting the vesse! in an ice bath. The beakers, which had been cleaned and heat dried, just previously, and one of which had been marked with the index cross, were partially filled with this gas-iree water. So far as could be judged by the eye, heating this water form- edi the indéx cross as’ plainly. as it had been formed ' in. other, tests where tap water had been used. Of course practically no bubbles were formed on any other part of this beaker and none were formed in the other beaker. These simple tests do not give any information as to the composition of the bubbles. They may be made up of air which had beem adsorbed: by the vessel: due. to the: presence of the 78 THE KENTUCKY ACADEMY OF SCIENCE organic material, or they may have been made up wholly oi the organic material in a gaseous state. Severa! ccm. of this gas were caught above the water by means of a funnel. No analysis of the gas was made but it is evident that if the gas had been of organic composition, it would have gone back te its liquid or solid state upon cooling. As this did not occur. it is concluded that the bubbies were composed of air. The tole played by the organic matter is not exactly cleary Wins matter may have been.the agency of air adsorption, or its pres- ence may have made the glass a good adsorber. The volume of gas given off indicated that the strong forces of adsorption came into play, but the precise manner of thei action is not evident. The tests show that no considerable amount of the ais within the bubbles could have come from the water, but that it came from the surface of the vessel itself. It is also showz by the tests that the amount of gas forming in bubbles is determined by the state of cleanliness of the vessel containing the water. It is hoped that quantitative results on these tests can be shown in a later paper. 13. Recent Developments in Oil Shale Technology. C. 5 Crouse, Dept. of Mines and Metallurgy, Univ. of Kentucky. (By title.) 14. A Reversed Compton Effect. T. M. Hahn, Physics Department, Univ. of Kentucky. Using an improved form of X-ray spectrometer designed in this laboratory, photographic spectra of the fourth order of the Molybdenum Kai and Kaz lines were obtained with a separation of 0.16 centimeter, where the width of the a, line on the photographic film was 0.01 centimeter. With this re- solving power there was no evidence of fine structure in eithe: oi or a2 line, but there was a pronounced broadening of both the a1 and a2 lines, resembling very much the Compton effect for scattering at a very small angle, but with a shift of ap- FIFTEENTH ANNUAL MEETING 79 proximately 0.0003 Angstrom unit toward the short wave-length side. Similar results were obtained in second order spectra. It is presumed that this indicates an actual absorption ot energy by the X-ray beam from the high-speed cathode stream Of -electrons, due to -collisions: betwéen X-ray quanta: and electrons, with a resultant change in direction and increase in energy for the quanta, and a corresponding loss in energy hv the electron, assuming that the ordinary relativistic laws of conservation of energy and momentum are valid in this. case as in the Compton effect. The increase in energy here observ- ed must necessarily be small, since only X-rays which had beer deflected thru a small angle could enter the slit system. . The energy of a quantum of Ka radiation, hc/d, is 2.76x10-5 ergs, whereas the energy Ve of an electron which has fallen thru a potential of 30,000 volts is 4.77x 10-5 ergs, nearly twice as much as that of the quantum. If one postulates an equipartition of energy in an equili- brium between quanta and electrons, as in two gases of different temperature mixed together, it is seen at once that all wave- lengths, up to the minimum A=hc/Ve would result. Thus this also indicates that the general radiation from an X-ray target may be due to a reversed Compton effect. 15. Note on the High Phosphate Areas of the Middle Cin- ‘cinnatian. S. D. Averitt, Ky. Agricultural Experiment Station. Small areas of soil, geologically well up in the Cincinnatian, containing distinctly more phosphorus than is usual in trat horizon, have been known for some years. Such soil was col- lected by the writer in 1916, on Bullskin Creek in Western and Northwestern Shelby County, Ky., always first or secoiid bottom, and associated with Arnheim limestone.* Samples from Shelby and other counties analyze from 0.2 to 0.7 per cent of phosphorus. Two samples of Arnheim limestone analyzed for the Ky. Geological Survey yielded 0.39 and 0.36 per cent of phosphorus, and a selected sample, 0.81 per cent. *Soil Survey of Shelby Co., Ky., Field Operations of the Bureau of ‘Soils, 1916, pp. 59 and 63. Elg Silt Loam and Huntington Silt Loam. 80 THE KENTUCKY ACADEMY OF SCIENCE The phosphatic character of this limestone is thought to ac- count for the unusual phosphorus content of these soils. The Arnheim limestone occurs above the middle of the Cincinnatian series, at the top of Maysville. 16. Verification of Lord Kelvin’s Theory of Radio-Fre- quency Resistance. R. B. Scott, Physics Dept.,. Univ. of Kyz (Abstract.) The first practical solution of the problem of alternating current resistance was given by Lord Kelvin in 1889. His solution has since been used for the computation of tables which give the ratio of alternating current resistance to direct current resistance for wires of any diameter. Various experi- menters have checked Kelvin’s solution for certain frequencies but the author has seen no extended experimental study of this subject. In view of this fact the work was planned to cover a considerable range of frequencies and wire diameters. The source of power used was a simple vacuum tube oscillator and the measuring instrument was a vacuum tube voltmeter. For the most part the results checked with the theory, with an error of less than two per cent. 17. Purification of Helium. D.%S. Hughes, Physics Dept. U. of Ky. 18. Series Spectra in Helium. Daniel Bailey, Physics Dept; Wot Ky. An analysis was made of the spectrum of neutral helium produced by a condensed discharge in a quartz discharge tuhe. and analyzed by means of a concave grating spectrograph. The formula: giving the position of any lime in aiy Series is a: follows: R Ge Mien Keeler s) — Moo kK -k (Me Kok) where R is the Rydberg constant and M, K and k depend upon the series under consideration. All the lines in the spectra of Parhelium and Orthohelium in the principal, first subordinate FIFTEENTH ANNUAL MEETING 81 and second subordinate series, which were in the range of sensitivity of the photographic plate used, were identified, with the exception of two lines in the second subordinate series of Parhelium. 19. The Value of the Acceleration of Gravity at Lex- ington:..S.C, Gladden, Physics Dept.; U.of Ky. A history of pendulum determinations of the acceleration of gravity was compiled, access being obtained to many rare papers dealing with the work of Kater, Sabine, Foster, and other pioneers in this field. An absolute determination of the acceleration of gravity at Lexington, Kentucky, was made, employing a chronograph and standard clock in conjunction with a Kater’s pendulum of late model. A cathetometer was employed to measure the distance between the knife edges, and corrections were made for temperature effects, arc of swing, and flexure of the pen- dulum support. The acceleration of gravity was found to be 979°2-em./séc?, with a. probable. error of 0.24-cm./sec?. 20. A Comparative Study of Delinquents and Non-Delin- quents. Clara Chassell (Cooper; Eastern Ky. State. Teachers Golllege=(Abstract.*) The method of the present study consists.in.a tabular presentation for paired delinquent and non-delinquent groups _of certain prescribed information obtained from studies made by many investigators, and the subsequent statistical reductisn of selected data by means of the calculation of coefficients cf colligation. Ninety-five coefficients were caiculated for the following groups: Feeble-minded, Adult Criminals, Juveniie Delinquents, Sex Offenders, and Alcoholics. The countries represented by these results are the United States, Porto Rico. the Philippine Islands, Canada, Great Britain, Ireland, Sweden. *See The Relation between Morality and Intellect: A Compendium of Evidence Contributed by Psychology, Criminology, and Sociclogy. (Address Bureau of Publications, Teachers College, Columbia Uni- versity, New York City.) bo THE KENTUCKY ACADEMY OF SCIENCE (oe) Germany, Austria-Hungary, Switzerland, Belgium, France, and Australia. The principal findings of the study are concerned with the relation between delinquency and mental inferiority, as disclosed by the following types of evidence: Reports of the Prevalence of Delinquency, Estimates of the Prevalence of Mental Deficiency, Reports of Educational Status (The Preva- lence of Illiteracy, the Amount of Schooling, School Progress. Educational Achievement), and Results of Intelligence Tests (Tests of Verbal, Abstract Intelligence, Arnrv Mental” Tests, Tests of Non-Verbal Concrete Intelligence, Tests of Mechar- cal Intelligence). The central tendencies of the coefficients for the several groups consistently disclose a relation between delinquency and mental inferiority in each of the types of evidence tabulated. The degree of relationship indicated, however, varies from group to group and from one type of evidence to another. The median of the ninety-five coefficients representing all groups and all types of evidence is .48. The study is a part of a research of much wider scope on the relation between morality and intellect. Uhe central’ ten- dencies of the correlational results for all parts of the research support the finding of a positive correlation between delin- quency and mental inferiority, and at the same time reveal! a positive correlation between moral character and intelligence: but suggest a somewhat lower degree of relationship between morality and intellect than that indicated by the central ten- dency of the correlational results for the comparative study of delinquents and non-delinquents. 21. The Results of a Consanguineous Marriage. R. G Will, Centre-College. A young man, unwittingly, married his half sister. Both apparently, were of normal intelligence, as were their parents. Eleven of the descendants were feeble minded, four being in the third generation, one in the fourth and six, probably seven FIFTEENTH ANNUAL MEHETING 83 in the fifth, the economic situation in each generation 1s worse than in the one before. 22. Some Phases of Measurement in the Field of Latin.* R. Tyson Wyckoff. The test movement may be said to have been born with the present century and to have-originated in the simple spel!l- ing lists or embryonic tests evolved by Dr. J. M. Rice in 1894-5. His idea that a test on a part may indicate proficiency in the whole met with a storm of disapproval and he was unable iv give a scientific base to his contentions. His arguments, how- ever,..were of such sort’as to draw the attention of Dr. E, 1. Thorndike and to result in studies on the theory of educationai measurement and statistical method which culminated in Dr. Thorndike’s Mental and Social Measurements, published in 190+, and in the various tests and scales prepared by his students for use in the elementary school. The first noticeable effect of the test movement in the fie!d of Latin or in the other high school subjects occurred when Dr. Gonzales Lodge counted the frequency of Latin words in Caesar’s De Bello Galico, books I-V; the six commonly read ora- tions of Cicero; and Vergil’s Aeneid, books I.-VI. When he pub- lished his statistics in the Vocabulary of High School Latin in 1907;;-figures’ for the first time were made available whereby. a teacher might determine which words were most common and therefore most deserving of emphasis in preparation foi Caesar, Cicero, and Vereil:- ‘Lodge's, methods of vocabulary analysis were imitated in Byrne’s Syntax of High School Latin.— a study of the frequency of occurrence of the various types of syntax in Caesar, Cicero, and Vergil. *The content of this paper was based upon a tabulated analysis of sixty four Latin tests, twenty-nine of which are partly stan- dardized and thirty-five non-standardized tests composed by seven different authors. All the non-standaidized tests were made in 1925 or later. It was necessary in making the tabu- lation to assume that an author’s failure to mention any cer- tain essential item of information constituted an admission that the particular test was lacking in just that respect. The tests were judged by the Otis Scale For Rating Tests. 84 THE KENTUCKY ACADEMY OF SCIENCE In 1912 Dr. Paul H. Hanus of Harvard decided. that; since the successful elaboration of elementary school tests had al- readv been demonstrated by Thorndike in handwriting and Stone and Courtis in arithmetic, it should be possible to apply test principles to measurement in Latin. Accordingly, with the help of three graduate students, he prepared Latin tests on grammer, vocabulary, and translation, with the endeavor of measuring growth in power in Latin and the correlaticn exist- ing between the three language skills assumed. Altho tire tests were an advance over the subjective tests of the past. they could certainly not be classed as objective in any true sense of the word. The author succeeded in indicating that, so-far as the tests showed, there seemed to be little corre- spondence between the types of abilities necessary for success in grammar, translation, or vocabulary. It is not required especially that we interpret this tack of correspondence as in- dicating failure of the three skills to function in Latin attain- ment. “. Dr, :-Hanus- did not: attempt’ to) establish@either sthe validity or the reliability of his tests, altho he did provide some tentative median norms of achievement by the various years. Dr. H...A. Brown, now President: of the’ State, Normal School, Oshkosh, Wisconsin, in 1915-17, elaborated tests on isolated Latin sentences, connected Latin, Latin grammar, and isolated Latin vocabulary. These tests were administered, to between 813 and 2,160 pupils and tentative median norms were obtained for each test by years. Neither reliability nor validity of the tests is stated in the published bulletins on the develop- ment of the tests. Dr. Brown attempted to score his connected Latin test objectively by dividing the passage into so-calied thought units, which were made to serve as a scoring key. The words of his vecabulary test were chosen from a list found to be common to seven elementary Latin texts. © He seemed to. make little attempt at objectivity im scoring the jsentence and grammar tests. The Starch Latin Tests appeared in 1915 and agaimasmtlie Starch-Watters Latin West in 1918. This test consists of FIFTHENTH ANNUAL MEETING 85 section on vocabulary, which was obtained by taking every twentieth word in Lodge’s list, and a section on translation of sentences which were found by selecting sentences located ai equal intervals in five first-year texts and in Caesar, Cicera. and Vergil. Median norms were calculated on five hundred pupils by years of Latin studied. The scoring was highly subjective. In general, the tests published between 1912 and 1920 dealt with translation, mostly of unconnected discourse cr vocabulary, and with syntactical analysis. No test had equiva- lent forms. Brown’s connected Latin Test seems to have been the only test with which a scoring key or suitable directions for scoring were provided. The objectivity of the scoring wis questionable. In most cases the spaces for answers were ss arranged on the page that error in scoring was likely. Ten- tative median norms were found' for each test. There were n directions for interpretation or application of results. No sample exercises preceded the tests to show the pupil the char- acter of the. construction of the test. There was no attempt to linait: atest to.a particular stade or year of Latin, Very litte endeavor was made to arrange the test content in order of difi1- culty. There was no manual with any test and no effort to obtain validity or reliability. a) The Henmon Latin Tests had been published in 1917 and nevisearin 1971 in revised torm: ‘ests li Ii ill) and) IV. became somewhat comparable. The scoring of these tests was more objective than that of previous tests and the spaces fer answers were arranged conveniently for scoring. For the first time a manual, directions for scoring, and a class record sheet were supplied, but there was no scoring key. In the pericd from 1920-24, among others were published the Pressey Latin Syntax .lest.: the Jiyler-Pressey atin Forms, Pest, andthe Ullman-Kirby Latin Comprehension in two forms in 1922; Briggs’ Semester Latin Test, Inglis’ Latin Syntax, Vocabulary. and Morphology Tests in several forms, the Lohr-Latshaw ltatin Forms Vests,>the Godsey~ Vatin Composition. ‘Test,’ the 86 THE KENTUCKY ACADEMY OF SCIENCE Stevenson Latin Vocabulary Vest, and the Stevenson-Coxe Latin Derivative Test in 1923; and the White Latin Test in 1924. These tests show considerable advance in objectivity simplicity of scoring, use of scoring key, preparation of equiva- lent forms, isolation of particular types or elements to alioxs for diagnosis of difficulties, lessening the amcunt of time re- quired for reading the preparatory instructions of the tests and for making explanations. Several of the tests give limited sug- gestions in interpretation, such as methods of obtaining medians and percentiles, and in application ot results, such as in class sectioning (White), comparison of individual’s or class stand- ing with absolute attainment (Inglis), tabulation of types oi errors for diagnosis (Godsey, Pressey, Tyler-Pressey, New York Latin Achievement Tests). Whereas, from 1912-20 most of the tests either had not been published for distribution or were sold by the test or by the hundred tests, it came to be the custom to put them up in packages of twenty-five. It may be rather interesting in passing to compare differ- ent methods of score derivation employed by two men in the same institution. Dr. Inglis felt that the words of his vocabu- lary test should be scored upon the basis of their frequency of occurrence. ‘That is to say, the more-often a word is met. the more important the word and the greater the amount the student should be penalized for not knowing the word Then, the commoner the word, the higher the score to be assigned to it as its value. In line with about the same type of reason- ing, Dr. Hanus also felt that his vocabulary test should be scored upon the frequency of occurrence of the words. Accor¢- ingly, a word which is met frequently is less difficult than an- other word met less frequently. The rarer the word is and therefore the more difficult, the greater the assigned score should be and the commoner the word is and therefore the less difficult, the lower the score must be. Apparently we have no great reason to consider those two vocabulary tests equiva- lent, except in inverse ratio, unless we assume that the fact the authors were both teachers in Harvard would be sufficient justification for concluding reliability. FIFTEENTH ANNUAL MEETING 87 During the period from 1920-24 Latin test development seemed to be directed toward refining and meking mere care- ful application of test principles. The Ullman-Kirby Latin Comprehension Test measures understanding ot passage con- tent much more skilfully than its predecessor, the Brown Con- nected Latin Test, which was striving awkwardly after the same aim: Moreover, the former test is somewhat more ob- jective than the latter. . The. Ullman-Kirby,; the Godsey, the Pressey, and” the) Tyler-Pressey.; seem: .to: be of some value. altho no one of these may be considered as a highly depend- able measure when used alone. It is desirable to employ a Latin achievement test in comparing class standing thru suc- cessive semesters or years, in the same classes or in different classes, schools, or systems; but when the individual is judg- ed, one should have an average of several tests. of one or more tests and a habit-rating scale, teachers’ marks, teachers’ rank- ings, or some other means. Since the publication of the Classical Investigation Report +1 the spring of 1924 the quality of Latin tests has been on the whole higher than in the two preceding periods. The Deferrari Test in Vocabulary and Forms, so far without norms, and the ‘Deferran’ Test in atin’ Comprehension, with norms ‘tor the fifth and seventh semesters. were published in 1925. The Orleans-Solomon Latin Prognosis Test (1926) and the Wyckoff Latin Prognosis Test (1927) both show marked reliability and validity and pre-high school norms. The New York Latin Achievement Tests for first and second semester pupils were published in 1928 with norms but with no stated validity or reliability. Each of the two New York tests is a composite of a number of sections which contain but few items per section. Several of the tests published since 1924 are incon- venient. to use because they are too long to be given in the ordinary class period. The most promising aspect of Latin test development has proceeded directly from the emphasis placed upon test con- struction, by the’ Service’ ‘Bureau For: Classical “Veacheis. 88 THE KENTUCKY ACADEMY OF SCIENCE Columbia University, New York. Under the influence of the Service Bureau excellent non-standardized tests have been made by Dr. Mason D. Gray, Miss Simpson, Miss Downes, and others. These tests for the most part caver phases which have been dealt with very little or not at all in previous tests. Some of these phases are the cultural, the historical, deriva- tives, general language ability, quotations, Latin in English spelling, Latin phrases, prefixes, rhetorical figures, word order. In the Service Bureau tests we find the following types of test organization: completion, recognition, classification, cld-type translation, recall, true-false, matching, lsting. analogies, op- posites, ranking. Two criticisms to be made of the Service Bureau tests are that, while the tests are skilfully prepared in point of choice of material for inclusion, the number of items in any one test is probably too few. Also, in some instances three or four different types of test development are employed in the same test, so that gross item scores of the various parts are certainly not at all comparable. Both objections may be miet,,1i a seriesof tests’ be given -and the items ‘or alliithesseries be classified according to the different types of test develop- ment employed. Then, when all items of each type are added. there will be a sufficient number of items that the scores for each type may be thrown into a frequency distribution, an.ap- proximation of the normal curve applied, and consequently the errors pointed out in the two objections may be avoided. The, Holz tests and: “the “Sellers“tests ‘of them@mponia Kansas, State Teachers’ College and the North Carolina High School Senior Examination (Section H on Latin) are among the most recently published non-standardized. The same ob- jections may be raised to these as were made in respect to the Service Bureau test. In addition, the Holz tests and the Sellers tests, which are composite tests, do not seem to preserve proportion in the amount of space devoted to the several skills dealt with. The North Carolina Examination puts an undue amount of emphasis on isolated vocabulary, very little stress upon comprehension, even of isolated sentences and compre- hension of connected discourse is completely ignored. FIFTEENTH ANNUAL MEETING 89 It would seem for effective test making that there is great need to determine what are the Latin aims (probably suffi- ciently handled in the Classical Investigation Report, Vol. III.), what skills are concerned with the aims, how much of each skill we shall want acquired, and what activities enter into the ‘desirable skills’. -We may approach the matter by -dis- integration of aims to obtain activities or by building up ac- tivities to obtain aims. The latter method will presuppose that existing activities in Latin are worth-while activities and that they will lead to desirable aims. We have great need for Latin tests which will really begin to reach the Latin objectives rather than merely the kind of tests we have now which deal with small skills that enter into the Latin objectives we do not know how much or how Httle. BIBLIOGRAPHY OF PUBLISHED TESTS Anderson, F. M.: A True-False Test For Comprehension of Archias. Service Bureau For Classical Teachers, Coiumbia University, No. 198. IRR WARS elke An Examination in First Term Latin. Classical Weekly, March 19, 1923, v. XVI., No. 19, Whole No. 442. *Brown, H. A.: Connected Latin Test, Latin Sentence Tests, Latin Grammar Test, and Latin Vocabulary Test. Are contained in Brown, H. A.: Latin Im Secondary Schools (1919) and A Survey of Instruction In Latin In New Hampshire Secondary Schools (1921). State Normal School, Oshkosh, Wisconsin. *Deferrari-Foran Latin Comprehension Test. 1925. The Catholic Education Press, 1326 Quincy St., Washington, D. C. Deferrari-Foran Tests in Vocabulary and Forms, 1925. The Catholic Education Press, 1326 Quincy St., Brookland Sta., Washing- ton; D:. €; Downes, Juanita: Latin Tests For the Highth Grade. March, 1928. Service Bureau For Classical Teachers, Coitmbia University, No. 302. Downes, Juanita: Latin Tests For the Ninth Grade. March, 1928. Service Bureau For Classical Teachers, Columbia University, No. 303. *Godsey, Edith R.: Diagnostic Latin Composition Test. 1922, World Book Company, Yonkers-on-Hudson, New York. *Has norms. 90 THE KENTUCKY ACADEMY OF SCIENCE Gray, Dr. Mason D.: A Written Lesson to Test the Pupil’s Under- standing of the Background of the Orations Against Catiline. March, 1928 Service Bureau For Classical Teachers, Columbia University, No. 305. *Hanus, Paul H.: Latin Tests (Vocabulary, Translation, and Gram- mar). 1912. Found in Hanus: School Administration and School Reports (1920). Houghton, Mifflin Company, Chicago, Illinois. *Henmon, V. A. C.: Latin Tests. 1917 and 1921. World Book Com- pany, Yonkers-on-Hudson, New York. **Inelis, Alexander: Latin Morphology, Latin Syntax, Latin Vocabu- lary. 1923. Ginn and Company, 70 Fifth Avenue, New York. *Lohr-Latshaw Latin Form Test. School of Education, University of North Carolina, Chapel Hill, North Carolina, 1923. *New York Latin Achievement Test. 1928. World Book Company. Yonkers-on-Hudson, New York. North Carolina High School Senior Examination, Section H. 1927. School of Education, University of North Carolina, Chapel Yill, North Carolina. *Orleans-Solomon Latin Prognosis Test Form A. 1926. World Book Company, Yonkers-on-Hudson, New York. *Pressey, L. W.: Test In Latin Syntax. 1922. Public Scnvol Pub- lishing Company, Bloomington, Illinois. Simpson, Elizabeth: Test on Word Order in Latin. March. 1928. Service Bureau For Classical Teachers, Coiumbia University. New York. No. 307. Simpson, Elizabeth: A written Lesson For a Review of Rhetorical Figures in Vergil, March, 1928. No. 306. Service Bureau For Classical Teachers, Columbia University, New York. *Starch-Watters Latin Test. 1918. Published by Dr. Danie! Starch, 1374 Massachusetts Avenue, Cambridge, Mass. *Stevensen Latin Vocabulary Test. 1923. Public School Publishing Company, Bloomington, Illinois. *Stevenson-Coxe Latin Derivative Test. 1923. Public Scheol Pub- lishing Company, Bloomington, Illinois. *“Pyler-Pressey Test in Latin Verb Forms. 1922. Public Scheol Pub- lishing Company, Bloomington, Illinois. *Uliman-Kirby Latin Comprehension Test, 1922. Extensicen Divi- sion, University of Iowa, Iowa City, Iowa. *White Latin Test. 1924. Worid Book Company, Yonkers-on-Hudson, New York. *Has norms. **Some of the forms have norms. FIFTEENTH ANNUAL MEETING oi 23. The Psychological Processes In Learning History In The Secondary School. M. E. Ligon, Education Dept. Univ of Kentucky. The definitions, conceptions and forms of history are nouz fixed and therefore the psychology involved in the study of history will be determined by. the point of view adopted. Opinions differ as to what the aims of history teaching shall be. This further complicates the psychology of its teaching and study. The author has taken the generally accepted view in the methods and processes and the common methods of teach- ing. He points out that imagination, memory, judgment, rea- soning, and sympathy are involved. 24. Some Cases of College Vocational Guidance. G. C Bassett, Psychology Dept., Univ. of Kentucky Two cases were reported informally. The paper will be published elsewhere. 25. Certain Factors Contributing to the Delinquency of Reform School Girls. Mrs. Juanita Curry Boyuiiton. The study was made of all girls confined in the Kentucky Houses. of Reform at Lexington, within the ages of 12.10.16 years, inclusive. It was not intended to inciude all tactors. Seventy-one per cent of the girls had lived in houses where abnormal parental conditions existed. The median number of children in the families was 8.25. Forty-eight per cent of the girls and twenty-eight per cent of their mothers had held posi- tions outside the home, mostly of a servant nature. The average school grade was the sixth. More than 75 per cent of the girls had one or both of the social diseases. The conclusion seems justified that these girls reached their delinquent careers thru a more or less watural: process when low mentality was forced to react to poor environment. 26. Kentucky Fluorites. W. R. Jillson, State Geologist, Frankfort, Kentucky. Within the last’ few years the mineral fluorite (CaFe). 92 THE KENTUCKY ACADEMY OF SCIENCE commonly referred to as fluorspar, has become of much eco- nomic significance in Kentucky. Occurring in commercial quantities in two general localities, the North-Central Ordovi- clan outcrop and the western tip of the Mississippian pieateau its production has steadily increased until at the present time Kentucky stands first in the United States and the werld. In 1926 Kentucky produced 62,459 tons of commercial fluorite valued at $1,167,129.00. Altho the volume indicated is considerable it is certain that much more fluorite could have been produced in Kea- tucky had it not been for the rather considerable foreign 1m- portations. In fact, during recent years, post war industria! and shipping conditions abroad have so materially aided the importation of foreign fluorite as to elevate it to the point of a rather serious menace to the domestic fluorite mining in- dustry. This is particularly true in Kentucky. With this fact in mind, the’,Director of the Kentucky Geological Survey while in Spain during May and June, 1926 attending the meetings of the 14th International Geologica: Congress, entered into relations of mineral exchange with many of the National Geological Surveys for the purpose of secur- ing representative specimens of cabinet size from all commer- cial “and smaller’ deposits of: fluorite, “At “the same timesand later, one or two important fluorite collections were purchas- ed to which many recently obtained specimens from points widely distributed thruout the world have been added. As a result of these endeavors it is thought that the fluo- rite collections of the Kentucky Geological Survey are probably of the first order in point of geographic representation and variability of mineral occurrence. All known commercially pro- ducing and scientific localities are represented by one or more specimens, duplicates, triplicates and quadruplets of some areas being a-part of the collection’s reserve. In this unique and beautiful cabinet displayed in the office of the suite oi the Survey at Frankfort, the Kentucky fluorite is well repre- sented by over 125 specimens. The entire fluorite cabinet con- FIFTEENTH ANNUAL MEETING 93 tains over two hundred separate trays. Thirty-five other states and foreign countries are represented, the complete list being as follows: Albuquerque (New Mexico), Arizona, Baden, Ba- varia, Bohemia, Butte (Montana), California, Cayote Springs (New Mexico), China, Colorada, Connecticut, Cornwall (£ng- land), Crystal Peak (Colorado), Derbyshire (England), Dur- ham (England), France, Freiburg (Germany), Germany, Green- land, Hastings County. (Ontario), Pennsylvania, Rhodesia, Rochester (New York), Salzburg (Germany), Saxony, Siberia, South Africa, Sydney, St. Gothard (Switzerland), St. Lawrence County (New York), Spain.) Switzerland); Vennessee, Valoes (Switzerland), Weisseck (Germany) and Windhoek (South chiICa’)!. 27. Racial Specialization of Parasitic Fungi. FE. C. Stak- man, Univ. of Minnesota and U. S. Dept. of Agriculture. Many species of fungi which cause plant disease comprise distinct physiologic races or parasitic strains, usualiy known as physiologic forms. The phenomenon of physiologic speciali- zation has been known for many years, first having been demonstrated clearly by Eriksson, a Swedish investigator, in 1894 Hlowever, the extent.of the phenomenon and its tar- reaching consequences in mycology and plant pathology have not been appreciated until recently, and probabiy are only 1m- perfectly appreciated now. Physiologic specialization is common in many groups of very destructive plant pathogenes: the rusts, the smuts, the powdery mildews, the fungi which cause root rots of cereals, the organism causing flax wilt, and a great many others. In order really to understand the course of development of a plant disease, and especially the development of epidemics, it is essential to know the number, geographicel distribution, pathogenic capabilities, and ecological peculiarities of the dii- ferent physiologic forms of the causal organism. An investiga- tion of physiologic specialization must become regular pro- cedure in the investigation of plant diseases. 94 THE KENTUCKY ACADEMY OF SCIENCE Physiologic forms can be recognized in several different ways: (l) By. theireffect:on) host, plants; (2) ‘sometinresssb shght morphologic differences between the different forms, al- tho these differences are not sufficiently great to justify cali- ing the forms species or varieties; (3) by differences in cultural characters; (4) by their reaction to physico-chemical environ- ment, such as temperature and hydrogen-ion concentration. Certain obligate parasites, like the rust fungi and the powdery mildews, can be recognized best by their effect on certain selected, varieties of ‘crop, plants: The’ morphologt: species Puccinia gramins, the fungus causing the black stem rust of cereals and grasses, is a good example. P. gramums tritici causes infection on wheat and barley, but not on oats and rye. Puccinia graminis secalis causes infection on rye and barley, but not on wheat and oats, while P. graminis avenae causes infection on oats, but not on wheat, barley, and rye. But P. gramims tritici in itself comprises more than 40 parasitic strains which can be recog- nized by their effect on certain varieties of wheat; P. granumis secalis consists of at least a dozen forms which can be recognized by their action on certain varieties of rye, and P. graminis avenae consists of parasitic strains which can be recognized by their effect on certain varieties of cats. Physiologic forms of facultative sapropnytes often can be distinguished by their appearance in artificiai culture. The cultural characteristics of a given form are remarkably con- stant on the same medium and under the same conditions. 3ut on different media the same form may behave entirely differently. Furthermore, ditferent forms may look aimost exactly alike on certain media, but may ‘be entirely different in appearance on certain other media. This means, of course. that in order to differentiate forms it is necessary to grow them under proper conditions. Forms may differ also in their physico-chentical reactions. Some of them may have a low optimum temperature, while that of others mzcy be very much higher. | Obviously, this has an important application in controlling diseases by regulating the time FIFTEENTH ANNUAL MEETING 95 of planting of certain crops. Many of the forms differ also in their reaction to hydrogen-ion concentration and in their fermen- tative abilities. The question naturally arises as to how titese physiologic forms originated, whether they still are originating, and whether they are stable. Several theories have been advanced regard- ime theorioin: of -forms: - (1), Ecological adaptation 3.(2) hybridization; (3) mutation. Many of the earlier investigators, and even some at the present time, asserted that ferms might arise as a result cf association with certain host plants, a sort of ecological adap- tation. These investigators were of the opinion that physio- logic forms were very unstable and that their parasitic capabui- ities could be changed at will by keeping them on certain hos: plants. This idea probably is erroneous. Physiologic forms of many fungi are just as stable as morphologic species. This has been demonstrated clearly in experiments made at Minne- sota. Furthermore, we have collected the same physiologic tormm!.of, PP, gramiuus initict im. India, . Hungary, France, “the British Isles, and on the North American continent. Another form was collected in Japan, France, Norway, the British Isles, United States, and Canada. The parasitic behavior of each of: the forms was: the same regardless of the place i which it was collected. There is a strong probability that forms may originate thru. hybridization of previously existing forms;:, but. this has not been demonstrated for plant pathogenic fung? Many fungi apparently niutate abundantly. The so-called mutants probably are similar to bud mutations in higher plants. They often appear as pie-shaped sectors in colonies growing om artificial media. The frequency of mutation can be influenced by environmental conditions, such as amount and kind of nutri- ents and temperature, The mutants may also be ‘different pathosenically:;. some of them “are less virulent than. their parents, some of them more so, and some of them about equal- ly virulent. THE KENTUCKY ACADEMY OF SCIENCE it~) for) Physiologic specialization is important in piant quarantines. In epidemiology studies, forms may be important as biological reagents, and in attempts to breed disease-resistant crop plants. It is essential to attempt to prevent the introduction into any country of parasitic strains more virulent than those now there. It is known that the parasitic strains of certain patho- genes in foreign countries are much more virulent than any which now occur in the United States. Obviously, therefore. they should be excluded by quarantines. And certain other countries have good reason to exclude some of our parasitic strains. It has been shown conclusively that certain varieties of crop plants are resistant in some regions and susceptible in others because of the existence of different physiologic forms in different regions. Futhermore, the distribution of physio- logic forms can be used as a criterion of the source of inocu- lum in certain areas. Physiologic -forms have been used to identify certain varieties of crop plants. In order to make sure of the varietal identity of some whieats, it often is necessary to inoculate them with the proper physiologic form of rust. It is obvious that it is essential to take into consideration physiologic forms in the production of disease-resistant varie- ties of crop plants, because varieties and hybrids may be immune from or resistant to certain forms of a pathogene and complete- ly susceptible to others. Therefore, the parasitic capabilities of all physiologic forms of the pathogene must be known and attempts must be made to combine in one variety resistance to all of them. INDEX A comparative study of delinquents and non-delinquents, Cooper AXStiId yO moral judd srnemv; MEsOyaCO 222s eee ee AIrvadsorpbionm IMs Walter VESSELS, srtdie so. 220 sce Mere ee ele see swe eeee need caalete Amburgey, L. V., Chicken feed and MOI -............. cece cece eee PASIAN SSE Oil CAINS oe os ON Be SE Sn Us ed ee Anderson, W. A., Jr.. McHargue and Hill, Canebrakes of Kentucky and cane as a forage plant Acreversed. Compton eftect, Plaka 22.2 2 see A ACcstudy. orvmorall judgment, iIBoymtom. 2.222..: 2 es Atmospheric electricity, a study of, Henry —.-..0002202. eee Averitt, S. D., The high-phosphate areas of the Middle Cincinnatian Barley, D:. Series Spectra dim helium 2 Barkenbus, C. A., and Zimmerman, Chemical analyses of the bean and pod of the Kentucky coffee nut tree _..2 2. Bassett, G. C., Some cases of college vocational guidance..........._.... Bear, R. M., Factors affecting the success of college freshmen Beckner, L., Utica shale in Kentucky Bibliography Ol testsy tml, Waitrose 5 ee es 2 oe ae Borman, E. K., An organism isolated from the feces of a colitis LU LLG Gag es OS a SINS UE GALE SNR ee OS ie Boynton, Mrs. J. C., Certain factors contributing to the delin- quency ot -Retorm “School: girls 2: v2.1 ee ee Boynton, P. L., A study of moral judgment Breeding. Lede clover. cher ews, 2.280 eo gels aie Oe SU aor Soni Buckner, G. D., Hegshell-tormine materials. 2...) Burroughs, W. G., Geographical distribution of cancer mortality AUN es Wi GUC easy SS a ioe Se SB eo EY ne Sha et, ee UN ota ae Week hn Burroughs, W. G., Prehistoric forts of Kentucky HESS UW Sy eee Bd eS Di, Tans LOE, eae a een aed ts eee JAN uae ee tac Leela | Cancer mortality, causes affecting the apparent distribution of, in Kentucky, Burroughs Came Chicmmi Cala mallyiSes Oi we eos eve ee eR ee Canebrakes of Kentucky in prehistoric time and the importance of cane as a forage plant, McHargue, Hill and Anderson, Jr. Causes affecting the apparent distribution of cancer mortality in Kentucky, Burroughs @ellsanermae aout yee eee NN WR Ae le diel ARMac Cellsisas aC OlMOlGsS VSteMiSh os a5. ae ee ie EU eel 2 tA Certain factors contributing to the delinquency of Reform School SS Ae GVM OMe te ee sees ie as ae ee Be SO MMU ee CCH AUN atl Chemical analyses of the bean and pod of the Kentucky coffee-nut tree, Barkenbus and Zimmerman Chicken feed and mold, Amburgey COMO TA SPs ly.0 Po Mila CBee ao se oie SANDE SO ae Oe Piensa sill ot ce) tl ce Constitwubiomss aim Gey erwin eee SVE Cees 8 Se SIN a Constitution Zok livin es matters sMISChen sects selec ee) Cooper, C. C., Comparative study of delinquents and non-delin- PULTE TOE Spe Se NS Bo adie 8 Ce Ue CUA oa Le EMA Uy iy Crouse, C. S., Recent developments in oil shale technology Crouse, C. 8., Recent oil shale development —....20..0-2000022oo oe Davies, P. A., High pressure and seed germination Davies, P. A., Production of CO, from irreversibly injured cells Dimock, W. W., and Healy, Mycobacterium paratuberculosis bon So oo Ol a1-1 be O% poral 1-9 co bo O11 e Ne) a 32 73 47 5 47 81 78 43 30 al 30 $d ’ THE KENTUCKY ACADEMY OF SCIENCE Eitect of chlorides on the chlorine content of tobacco, Karraker.... geshiell_Tormine MateriaissBUCkMNiCT === 2 ee ene irikson, Miss 38., Recent developments in investigations of AVG A TY L1Sy Saroes ahe tiene On ep TNO FA Rc et! whet Ae lS a eee Experiments in methods ot inoculating soybeans, Karraker.............. l'actors afiecting the success of college freshmen, Bear ~.........0........ Mer AUS se Live Nee mised TMS Teds CLO, Crise lols see ee Ts ace ey eee Ie eee nn bisecher) Martin Ey’ Constitution of living’ matter). Ss TANTO TAGES, Te MiG CK yay eS © ray eee enee ete eins et a IE ee ae eae ea rourteenth international geological congress, Jillson Hungi, Racial specilization of parasitic, Stakman ~.........1..4.222. Gsological congress, 14th international, Jillson = 222 Geologyrot@the (sland Creek.oil pool. sillson Sates oe se eee Germination of seeds, effect of pressure on, Davies —......020..2.0.- Gladden, S. C., value ot the acceleration of gravity at Lexington... Gravitawlom. MEG aye oe PEA ee A eel ee Gravity, value of the acceleration ‘of, at Lexington, Gla dden siete Hahn Vis As reversed \Womptonverect, 2 a ee plea lsyien Osea ron Aa: TielsVal CAtd Odes rae seetek ON ne i OT ee ee Healy, D. J., and Dimock, Mycobacterium paratuberculosis -...._..... Healy, D. J., and Hull, Mineral metabolism during pregnancy.......... Healy, D. J., and McHargue, Hemoglobin determinations -.....2........... Heim, -Puriti cation (Of, ENV SiiSS Wy ee es eee le Ee eee Hiehume Series: SpectT ay dn: fees a ee Se et eee Hemoglobin determinations, McHargue and Healy ARE Re oR Henry, RG. Ac study. of atmospheric iclectricity 24.22 2 ees High pressure and seed germination, Davies ...... o FAMED in Hill, E. S., McHargue and Anderson, Jr., Canebrakes of Kentucky andy Gane fase Loraeemplant: 0 ence 2s Se en ee ees Hire, CS Air. eee in NENA hess re SR RIS ce 5 A le ae eee Hull: F. E., and Healy, Mineral metabolism during rOSNaAN Gyles Riv aration eM arks Om este ee weet ONS Gen 1 SUN ee AUS SE eee Indicators, experiments with Island Creek oil pool, geology of, Jillson Isolation and cultivation of Mycobacterium paratuberculosis, Ve arlivea Nice MOT OC Kas tae eae are Ee ea eset er Jillson, W. R., 14th international geolcgical congress Jillson, W. R., Geology of the Island Creek oil pool -..-.............. ee SMISONGOV Ee es SNUG Kay TUT OGLUCS). 6 ea eee ee ewe 7 Johnson, . M., and Valleau, Effect of tok Karraker, P. H., Hifect of chlorides on EOD ACE Org es A FN SS ees Md 61 Ske Re ee Karraker, P. H., Experiments in methods of inoculating soybea Kentucky tniwroritess siligoms i 22 eh ae le Ee a ae ee atin. Bibliosraply. Ob bests yim: 2 aE Uae aes eae Latin, Some phases of measurements in the field of, Wyckoff........ Ligon, M. E., Psychological processes in learning history in the secondary school Living. matter constitution. ot, uscher = ee eee McFarlan, A. C., Structural features of the Bluegrass Region.......... McHargue, J. S., and Healy, Hemoglobin determinations —............... McHaregue, J. S., Hill and Anderson, Jr., Canbrakes of Kentucky andvcane:as’ avtoraceh plants 222s ee eee Membership list co OT ON boo HS INDEX Mineral metabolism during pregnancy, Healy and Hull -.................. Minutes on the (4th annwal meeting. ieee se So scsi Mimutes=ohe thiesd: 5th amniumalwmiee tiie sis. 2 bese eo oes ee eeeeteeceeee MoOUmtaAim: tora vot uentuckya Sim iti tnes.) moe Se ae ee Mycobacterium paratuberculosis, isolation and cultivation of, a Gay ares Cie) TINO Che ct-c i eees sete Hata eter ervey ewe se ENORAE Eee ane INFECT ONO Sayers ras esse ae aN a OU tie ood cutee ae ee ESS RWrsnie Pals vee use INORWOOds C22 Jk COOLER: MOLTCGX@ Lats aco OS irs eau A AMIE uel Note on the high-phosphate areas of -the middle Cincinnatian, PANTS FD tc et Lh ee UV OL OL 2 a ae OTPICERS ee VO G Lh ODER) | eels aie Ae Pee A eg a MRO SL Fare LAE Oilsshale. recent: development; Crouse 22.02) Ee ea Oxidation of sulfur in limed and unlimed soils, Shedd Papers: read atthe