ah niece ib rane ghrnsnetie® © ee ete « ie ar ST ean bad Pie Ped Jiresented ta Che Library of the University of Coronto b Wo The Ontario Research Foundation JOURNAL OF THE American Veterinary Medical ‘ Association FORMERLY AMERICAN VETERINARY REVIEW Original Official Organ U. S. Vet. Med. Ass’n. Edited and Published for THE AMERICAN VETERINARY MEDICAL ASSOCIATION By J. R. MOHLER, WASHINGTON, D. C. EXECUTIVE BOARD George Hilton, 1st District; T. E. Munce, 2d District; S. E. Bennett, 3d District; J. A. Kiernan, 4th District; C. E. Cotton, 5th District; R. A. Archibald, 6th District; Cassius Way, Member at Large. SUB-COMMITTEE ON JOURNAL S. E. Bennett J. A. Kiernan _Index to Volume LX NEW SERIBS VOLUME 13 October, 1921, to March, 1922 WASHINGTON, D. C. 1922 | fq > — 1 i A! f 4 tes = ™ \ is | oN ‘ i ie : X 65738 ee ea Uo el S7 nT 7 all / RO ali t De % Ccestecy Sass ih q 3 NS 4 t A x ~ AnAS. an* al = - naa UO a Cfeccacett SS det “iy, ‘ One aw om ‘ CTT peat LIST OF ILLUSTRATIONS Portrait oF Dr: A. T. KIvsSLrey. 8. Portrair oF Dr. Davin S. WHITE, 10. COMPARISON OF ABORTION IN VACCINATED AND UNVACCINATED CATTLE IN WISCONSIN EXPERIMENTS (CHarT), 31. VETERINARIANS ATTENDING TUBERCULOSIS SHORT CourRSE aT NorrH Dakota AGRICUL- TURAL COLLEGE, 112. BRONZE STATUE OF JUSTIN MorGan. 261, BuFFER EFFECT oF HorsE SEMEN (DIAGRAM). 296, Docs AFFECTED WITH ANTHRAX, 324. SHEEP AFFECTED WITH STOMACH WorMs, 326, INTUSSUSCEPTION IN A COLT. 329. GENITAL INFECTIONS IN A Butt: . Figs. 1 and 2. 422. Figs, 2 and 4, 423. Figs. 5 and 6. 424, EMBLEM ADOPTED BY AMERICAN VETERINARY MEDICAL ASSOCIATION, 501. A SHETLAND Pony THAT DIED OF TUBERCULOSIS, 601. PREVENTION OF LIVESTOCK SHIPPING LOSSES: Fig. 1. Diaphragm and Lungs of Hog, 618. Fig. 2. Apparatus for Sprinkling Hogs, 619. Fig, 3. Thin Cattle, 623. PorTRAIT OF Dr. DUNCAN MCEACHRAN, 625. RESULTS OF OPHTHALMIC TUBERCULIN TEsT, 735. THE FirrH MEpIcaL CoNGRESS OF CUBA: Portrait of Dr. Bernardo J. Crespo, 774. Group of representatives of medical and veterinary professions, 777. Group of special meetings, 777. ‘ CONGENITAL ANKYLOSIS IN HIND LEGs OF CALF, 784. lil Le Index of Authors Adams, John W., 144. Anderson, W. S., 291. Beach, B. A., 600. Beaudette, F. R., 283, 729. Bemis, H. E., 51. Blair, W. Reid, 756. Boerner, Fred, Jr., 83. Boyd, W. L., 84. Bushnell, L. D., 729. Butler, W. J., 59. Cahill, Edw. A., 702. Carr, George H., 194. Carroll, A. N., 315. Carter, Thomas B., 782. Cary, C. A., 329. Conley, C. C., 191. Covault,, Co H.,.. 67; Crawley, Howard, 461. Cross, Floyd, 562. Eichhorn, A., 771. Embree, W. J., 615. Evans, W. A., 683 Finkelstein, B. J., 208. Foster, J. P., 46. Gilman, Herbert L., 416. Glover, George H., 317, 767. Graham, Robert, 76. Hadley, F. B., 26, 600. Hadwen. S., 724. Hall, Maurice C., 583. Hayes. F. M., 435 Healy, D. J., 291. Henley, R. R., 717. Hollingworth, W. G., 610. Hopper, Earle B., 738. Hoskins, H. Preston, 453. Jakeman, H. W., 702. Jardine, Ernest F, 328 Jones, F. S.. 271. Jorgenson, G. E., 93. Kingman, H. E., 165. Lockett, S., 186. Lowe, William Herbert, 80. Lumb, J. W., 206. McEachran, Dunean, 761. MeNair, F. H.. 170. Moore, Veranus Alva, 625. Motley, A. A., 63. Munce, T. E., 402, 734, Munce, T. W., 702. Newsom, I. E., 562. Nighbert, E. M., 205, Noback, Charles V., 322, 32 Perry, Frederic M., 648. Peter, A. M., 291. Phipps, H., 435. Piper, H. B., 600. Cuinlany J--G:, 78. Ratigan, W. J., 96, 639. Records, Edward, 155. Reed, R. C., 691. Roberts, G. A., 177. Santa Maria, L., 306. Schroeder, E. C., 542. Schwarzkopf, Olaf, 382. Sisson, S., 736. Smith, H. R., 310. Stubbs, E. L., 83, 461. Torrance, Frederick, 22. Townsend, J. G., 606. Traum, J., 186. Troy, O. E., 299. Turner, John P., 640. Vawter, Lyman R., 155. White, David S., 9. il Vv Index of Subjects Papers: Abortion, Bovine Infections, Bureau of Animal Industry Investigations, 542. Abortion, Immunizing Cattle, 26. Abortion, Swine, 435. Address of President David S. White, 9. Animal Diseases, Importance of Preven- tive Measures, 402. Army Horses and Army Remount Service, 648 Bacillus Bovisepticus, Types in Dairy Herd, 271. Bacillus Ovisepticus Vaccines, Investiga- tions, 562. Botulism in Relation to Anti-Hog-Cholera Serum and Hog-Cholera Virus, 702. Botulism in Swine in Relation to Hog- Cholera Immunization, 76. Chicken-Pox Vaccine, Experiment to De- termine Value, 88. Clarification of Hog-Cholera Defibrinated Blood Antitoxin, 717. Colorado Veterinary Hospital, New, Dedi- cation, 767. Cooperation Between United States and Mexico for Control of Epizootics, 306. Corpus Luteum, Relation to Estrum and Involution of the Pathologic Uterus, 34. Cuba, Fifth Medical Congress, 771. Digestive Tract, Diseases, Dogs and Cats, 170. Garbage Feeding and Hog Cholera, 22. Genital Infections in Bull, 416. Health Departments Interested in Tuber- culosis Eradication, 683. Hemorrhagic Septicemia Organism, Viru- lent and Non-virulent Strains in Same Animal, 4538. Herd Efficiency from Standpoint of Vet- erinarian, 738. Hog Cholera, 691. Hog Cholera and Garbage Feeding, 22. Hog-Cholera Defibrinated—Blood Anti- toxin, Clarification, 717. Hog-Cholera Immunization and Botulism, 76. Hog-Cholera Serum and Virus, Botulism in Relation to, 702. Horse Semen, Hydrogen—Ion Concentra- tion, 291. Hoskins, W. Horace, Tribute to, 80. Hypoderma Larve, Effects of Improper Methods of Extracting, 724. Ictero-Hemoglobinuria in Cattle, 155. Laborattory Diagnosis of Poultry Dis- eases, 729. Laryngoplegia of Horse, Surgical Treat- ment, 144. Livestock,. Purebred, 194, Livestock Shipping Losses, Prevention, 615. Loco, Poisoning, Field Observations, 299. McEachran, Dean, Tribute on Behalf of McGill Veterinary Graduates, 756, McEachran, Duncan, and McGill Faculty of Comparative Medicine, 625. McGill Veterinary Reunion, Address, 761. Mexico and United States, Cooperation for Control of Epizootics, 306. Morgan Horse. Occurrence of Five Lumbar Vertebre, 379. Veterinarian and, Vl Municipal Food Inspection, 317. Parasitic Diseases, Treatment and Prophy- laxis, 5838. Parturient Paresis of Dairy Cow, 63, 200- Poultry Diseases, Clinical Aids in Diag- nosis, 283. Poultry Diseases, 729. Rinderpest in Brazil, 177. Sex Determination and Uterus, 46. Strongylidosis in the Horse, 67. Surgical Operations on the Ox, 51. Surgical Technique—Suturing, 165. Tuberculosis, Bovine, Control, 186. Tuberculosis Eradication, Bovine, Progress in Montana, 59, Tuberculosis Eradication, Cooperation, 310. Tuberculosis Eradication, Health Depart- ments Interested, 688. Tuberculosis in Shetland Pony, 600. Tuberculosis, Gbscure Lesions, 191. Tuberculin Test, Ophthalmic, Pennsyl- vania Instructions, 734. Veterinarian and Public, 315. Veterinarian and Purebred Livestock, 194. Veterinarian, Practicing, and Accredited Herd Work, 606. Veterinarian, Relation to Humane Work, 610. Viscera of the Cow, 736. Laboratory Diagnosis, Embryology of Clinical and Case Reports: Ankylosis, Congenital, Anthrax in Dogs, 322. Blackleg, Breed and Sex Susceptibility of Calves, 206. Capillaria Worms, Pathogenic Effects om Chickens, 461. Chorea in Dogs and Monkey, 639. Granulonia of Frog in Horse, 328. Intussusception in Colt, 329. Oleander Poisoning in Bear, 96. Parasites of Sheep on Sabana of Bogota. (Colombia), 325. Pericarditis, Traumatic, 208. Pyosalpingitis, Surgical Treatment, 93. Rupture of Uterus in Cows, 640. Swine Erysipelas, 781. Udders, Indurated, in Virgin Heifers, 205- in Calf, 782. Abstracts: Abortion, Infectious, in Mares, 464. ‘Anesthesia. General, in Horse by Intra~- venous Injection of Chloral Hydrate, 784. Anthrax, Diagnosis from Bone Marrow, 335. Antitoxins, Effect of Filtration on Po- tency, 102. ; Azoturia. Treatment with Animal Char- coal, 216. Bacillus Botulinus, Purity of Strains, 2138. ' Bacteria, Method for Determination of Number in Suspensions, 330. _ Beef Souring, Caused by Bacillus Me- gatherium, 214. Bipolar Infection in Animals, 785. Blackleg Immunization with Germ-free Filtrates, 465. American INDEX vil Botryomycosis, So-Called, in Man, 463. Calcium and Tuberculosis in Rabbits, 214. Cancer of Stomach in Horse, 334. Chaulmoogra Oil in Treatment of Tuber- losis and Leprosy, 385, 336. Cold, Action on Body, 466. Distemper in Pig, 215. Foot-and-Mouth Disease, 334. Foot-and-Mouth Disease 210 Fowl “Typhoid, Epizootie in France, 415. Gangrenous Infections, Acuie, Etiology, 212. Immunization, Nonspecific, Basis, (*2. India Lepartment of Veterinary Science, Report, 333. Johne’s Disease, Transmission to Experi- ' mental Animals, 784, Lumbago, Treatment with Animal Char- coal, 216. Meat of Healthy Animals Free from Bac- teria, 786. Oestrin in Pernicious Anemia of Hor ses, 783. Otitis Externa Parasitica of Cat, £1i. Paralysis, Lumbar, of Sheep, 44. Paratuberculosis (Johne's Disease), 78!. Paratyphoid of Honey Bee, 98. Pneumonia, Necrotizing. in Swine Plague and Hog Cholera, 466. ‘Septicemic Infection in Lambs Caused hy Swine Erysipelas Bacillus, 99. Stains, Polar, Preparation, 211. Strangles, Vaccination, 98. Suprarenin in Horse, 783. Swine Erysipelas and Erysipeloid (in Man), 645. ‘Tonsils as Part of Entry for Infections, 210. Tubercle Bacilli, Avian, in White Rat, 642. ‘Tubercle Bacilli in Bile, 100. Tuberculin, Diagnostic, 644. ‘Tuberculin, Diagnostic Value in Surgical Tuberculosis, 101. ‘Tuberculosis, Bovine, Complement Fixa- tion, 99. ‘Tuberculosis, Chaulmoogra Oil in Treat- ment, 335, 336. ‘Tuberculosis, Friedmann Treatment. 101. Tuberculosis Immunizing Vaccine, 463. ‘Tuberculosis in Small Rodents, 100. Immunization, in Switzerland, Human Army Veterinary Service: Army Veterinary Picnic, 219. Army Horses and Army Remount Ser- vice, 648. Percheron as Army Horse, 467. Regulations for Officers’ Reserve Corps, 341. Surgeon General Ireland, Letter, 340. Veterinary Corps, Examination for Ap- pointment, 103, 218. Association News: American Veterinary Medical Association Genver Doings, 239. Notes, 372, 508, 669, 802. Resident Secretaries, 667, Women’s Auxiliary, 241. Proceedings of Fifty-Eighth Annual Meet- ing Amendment to By-Laws. 369. Election of Officers, 350. ; Place of Next Meeting, 370, 493. Proceedings, 220, 349, 471, 657, 787. Report of Auditing Committee, 470. Report of Budget Committee, 502. Report of Committee on Abortion, 235. Report of Committee on Anatomical Nomeclature, 503. Report of Committee on culosis, 363. Report of Committee on Emblem, 500. Report of Committee on History, 234. Report of Committee on Intelligence and Edueation, 475. Report of Committee on International Vet- erinary Conference, 237. Report of Committee on Legislation, Report of Committee on Necrology, 35 Report of Committee on Resolutions, 503. Report of Editor, 787. Report of Executive Board, 223. 492, 500. Report of Representative on Advisory Hoan of Horse Association of America, 54 Report of Secretary, 228. Report of Treasurer, 231, 790. Sections, Joint Meeting, 657. Section on Education ‘and Research, 65 Section on General Practice, 657. 662. Section on Sanitary Science a Police, 657, 667. Section on Veterinary Colleges and Ex- amining Boards, 666. Special Committee on Prevention and Con- trol of Animal Diseases, 369. Other Meetings Alabama Veterinary Medical Association, 670. British Columbia Veterinary 520. California Board of Examiners, 375. California State Veterinary Medical As- sociation, 376. California Veterinary Practitioners’ ference, 799. Central Michigan Veterinary Medical So- ciety, 375, 325. Central Pennsylvania Veterinary Club, 250. Colorado Veterinary Medical Association, 525, 797. Conference of Research Workers in Ani- mal Diseases, 519. Connecticut Veterinary tion, 799. Georgia Veterinary Association, 246. Ilimo Veterinary Medical Association, 523. Bovine Tuber- 349. 471, Association, Con- Medical Associa- International Conference on _ Epizootic Diseases of Domestic Animals (Paris), 124, 251. Kentucky Veterinary Medical Associa- tion, 110. Maine Veterinary Medical Association, 798. Manitoba Veterinary Association, 374. Maryland State Veterinary Medical As- sociation, 114, Mississippi Delta 249, Mississippi State Veterinary sociation, 525. Veterinary Association, Medical As- Missouri Valley Veterinary Association, 106, 526. af Montana Veterinary Medical Association, 113 National Association of B. A. I. Veteri- narians, Metropolitan Division, 522. New York State Veterinary Medical So ciety, 243. North Dakota sociation, 111. Veterinary Medical As- Vill INDEX Oklahoma Veterinary Medical Associa- tion, 670. Ontario Veterinary Association, 118. Pennsylvania State Veterinary Medical Association, 377. Philadelphia Veterinary Club, 3876, 524, 669. Quebec Veterinary Medical Association, 251. Schuylkill Valley Veterinary Medical As- sociation, 524. Southeastern States Veterinary Medical Association, 524, 801. Tuberculosis Eradication Conference (Chi- cago), 258, 515. YVnited States Livestock Sanitary Asso- ciation, 510. University of Pennsylvania Conference of Veterinarians, 671. University of Pennsylvania Veterinary Alumni Dinner, 245, Western Michigan Veterinary Medical As- sociation, 245. Western New York Veterinary Medical Association, 113, 378. Wisconsin Veterinary Medical Association, 800. World’s Poultry Congress, 252. Editorial: Animal Experimentation, the Veterinarian and, 265. Antivivisection Antics, 268. Chiropractic, Veterinary, 142. Cuban-American Veterinary Entente Cor- diale, 681. Denver Convention, 1. Diseases of Livestock on Wane, 679. Foot-and-Mouth Disease, Immunization, 139. Foot-and-Mouth Disease Sweeps Great Britain. 677. Old and New Year, 399. Opportunities for Veterinarians, 400. President, Our Newly Elected (A. T. Kinsley), 7. Press, Annual Meeting and, 268. Rabies, Reminder of Real Danger, 269. Students, Veterinary, Enrollment, 537. Students, Veterinary. Shortage. 143. Tuberculosis Work, Additional Funds, 401. Vaccine Therapy, Efficiency, 141. Veterinarian and Public Health, 538. Book Reviews: Diseases of the Small Domestic Animals, Oscar Victor Brumley. 646. Italian Veterinary Annual, 647. Laboratory Manual in General Micro- biology, Ward Giltner, 216. Regional Anatomy of Domestic Animals, The Pig, E. Bourdelle, 337. Communications: Cuban Medical Congress, Invitation, 527- Ecuador, Letter, 256, Horses, Tractors and Trucks, 383. Mississippi, Exhibition in, 385. Morgan Horse, Five Lumbar Vertebrae, 379. low Fever, 674. Post-Card Advertising by Veterinary Sup- ply Houses, 255. Protecting the Stock, 121, Purebred Stock, 529. Watch Out for “Dr. J. Hanigan,”’ 255. Necrology: Albrechtsen, Jacob, 530. Archibald, R. A., 803. Armsby, Henry Prentiss, 530. Babcock, Charles H., 386, Barnes, F. E., 128. Edwards, W. C., 259. Gunn, Nelson T., 533. Harker, G. Frank, 123. Helmer, Jacob, 532, Jobson, George B., 672. Linn, Willard L., 673, Rectenwald. Mrs. N., 678. Robinson, Charles Barnwell, 672. - White, S. W., 387. Winsloe, J. A. H., 673. Miscellaneous: American Legion Adopts Tuberculosis” Resolution, 526. Animal Experimentation, the Doctor and the Dog, 91. Antituberculosis Vaccination, 282. Chiropractic, Veterinary, Again, 452. Food and Drug Law, Violations, 808. Government Scientific Periodicals Sus- pended, 676. Hassall, Albert, Honored, 760. Hookworm Treatment, 807. Horse Breeding, Move to Assist, 258. Horse for Street Cleaning, 21. Horses and Mules in War, 169. Horses, Endurance Test, 392. Horses Raise Cash, 534.. - Hoskins, Dean, Memorial Meeting, 805. International Congress on Comparative Pathology, 605. McEachran, Duncan, Honored Guest, 388. Morgan Horse, Famous, Honored, 261. Mountain Trip, 393. Rommel, George M., Resigns, 305, St. Kitts, Veterinary Practice in, 434. Salmon Memorial Fund. 259. South Dakota Laboratory Service, 671. Tapeworms in Dogs, Treatment, 396. Tuberculin Test by Area Plan, 395. Tuberculosis, German Savants on, 385. Tumors in Ethmoid Bones of Horses, 804. Veterinary and Human Medicine, Co- operation, 624. Williams, W. L., Compliment to, 339. Wood, Gen. Leonard, Fight Against Yel- low fever, 674. —~- JOURNAL OF THE American Veterinary Medieal Association FORMERLY AMERICAN VETERINARY REVIEW (Original Official Organ U. S. Vet. Med. Ass'n.) J. R. Mower, Editor, Washington, D. C. A. T. Kinsey, President, Kansas City, Mo. N. S. Mayo, Secretary, Chicago, III. M. Jacos, Treasurer, Knoxville, Tenn. Executive Board GEo. HILTON, 1st District; T. £. MuNce, 2nd District; S. E. BeNNetr, 3rd District; J. A. KrerRNAN, 4th District; C. E. Corron, 5th District; R. A. ARCHIBALD, 6th District Sub-Committee on Journal S. E. BENNETT J. A. KreRNAN The American Veterinary Medical Association is not responsible for views or statements published in the JourNaL, outside of its own authorized actions. _ Reprints should be ordered in advance. Prices will be sent upon application. Vor. 1x, NS. Vor. 13 OCTOBER, 1921 No. 1 THE DENVER CONVENTION THE fifty-eighth annual convention of the American Vet- erinary Medical Association was called to order by President David _S. White, in the Municipal Auditorium, Denver, on September 5, after an entertaining pipe-organ recital. The invocation was. delivered by Rev. James E. Davis, pastor of the local Central Christian Church. The assemblage was of- ficially welcomed to the city by the Hon. Dewey C. Bailey, Mayor of Denver, who related many interesting experiences of his fifty years in the cattle business, particularly those which he had with Texas fever in the early days when he trailed Texas cattle through Kansas and into Colorado. Colonel H. KE. Bemis, of. Ames, Iowa, responded happily and entertainingly to the mayor’s address of welcome. After several well-received vocal solos by H. W. Stanton, of Denver, President White delivered himself of a scholarly and well-prepared presidential address, outlining the work ac- complished by the Association within the last year and_ pre- dicting a steady growth for the future. This address will be found as the first paper in this issue. The first business session of the convention was opened in the afternoon with the report of the Executive Board, fol- lowed by the election of new members, the reports of the 2 EDITORIAL Secretary and of the Treasurer, and various reports of the different committees. Monday evening the President’s reception was held in the ball-room of the Albany Hotel, the headquarters of the con- vention. Dancing, with excellent music, followed until mid- night, and many members availed themselves of this oppor- tunity of rejuvenation. SECTION PAPERS On Tuesday morning the various sections of the Association met in joint session, beginning with an organ recital, and then proceeded with the reading of papers. Unfortunately many of those who were on the program failed to appear, but this was counteracted by the opportunity for more extended discussion of the papers of the authors present. Dr. Jakeman, Chairman of the Section on Sanitary Science and Police, who presided at the combined meeting of the three sections, noted the need of familiarizing the public with the work of the veterinary profession. The fact is not appreciated that the livestock sanitary officer is a veterinarian. The vet- erinarian should be credited with his service in protecting human and animal health, in addition to being recognized as one who treats sick animals. Whenever possible he should cooperate with the medical man. Dr. T. E. Munee stated that biological products are being used by veterinarians as a substitute for true prophylaxis, such as the primarily and fundamentally important sanitary meas- ures. Prophylaxis is always possible to some extent, even with- out full knowledge of a disease, though this knowledge is needed for formulating the best prophylactic measures. The pro- ducer of food is a highly important factor in preventing animal diseases. Pennsylvania has a veterinarian devoting his time to disease prevention. The application of known measures will control most, if not all, of our transmissible diseases. Dr. Munee read a series of sixteen principles for disease control and recommended that a commission be appointed to study the subject of prophylaxis and report on it. Drs. Records and Vawter presented a paper, read by the latter, on hemoglobinuria, and discussed the symptoms of the disease and reported the nature of the blood picture. There is a hemoglobinemia and a leucocytosis. The postmortem pie- ture was given in detail. The bacteriological findings are of Eprroriau 3 especial interest. A number of bacterial forms may be isolated from various eases of the disease, but Bacillus welchi was the only one constantly found present wherever examination was made under suitable conditions, and is the only one with hemo- lytic properties. Administered by mouth to cattle, it was in- effective in producing the disease, but intramuscular injections produced a number of eases in cattle. Apparently there are some unknown contributing or predisposing factors in the dis- ease, and it has a seasonal and regional incidence. Treatment was also discussed. Dr. C. D. Marsh gave a general talk of an informal nature, illustrated by lantern slides showing poisonous plants and poi- soned animals. He noted that whereas poisonous plants occur all over the United States, the losses in the West are much the more important, due to the conditions under which live- stock is kept. Cattle are turned loose for months and drift over areas of poisonous plants, and sheep are handled by herders who oceasionally herd them over dangerous areas through ignorance. Stock poisoning is usually the sequel to a searcity of feed, poisonous plants not being eaten from choice as a rule. There are few remedies that are of use under range conditions. Dr. Marsh exhibited specimens of whorled milk- weed, death camas, sneezeweed, rubber plant, high larkspur and aconite, and slides dealing with death camas, lupine, azalea, rubber plant, larkspur, sneezeweed, cherry and whorled milk- weed. Dr. Frederick Torrance’s paper, which was read by Dr. Hil- ton, reported the results of Canadian measures for controlling the spread of hog cholera by garbage. The law prohibits the feeding of garbage away from the premises on which the garb- age is produced, except under regulation and license. It is provided that such licensed persons must cook the garbage under suitable conditions and maintain a sanitary establish- ment. An outbreak of cholera is regarded as proof of viola- tion of the law in the absence of evidence to the contrary. This law works well in controlling hog cholera. Since its pas- Sage, Six years ago, 90 per cent of the outbreaks of hog cholera have been found to be due to violations of the law in regard to garbage feeding. Dr. George H. Glover reported that Fabre has been a greatly increased interest in the subject of municipal food inspection { Ki DITORIAL among people in general if not among the veterinary profes- sion. Such inspection is important in protecting the public health and therefore is more vital than streets, sidewalks, ete. Municipal inspection is feasible. The veterinarian must take an interest in this matter and other matters of public health. Dr. Glover gave a detailed account of the introduction of mu- nicipal inspection into Fort Collins, Colorado, noting the value of interesting the women’s clubs in this matter. Dr. Maurice C. Hall in his paper stated that we must take stock of our knowledge of treatments and preventive measures for the various parasitic diseases and adopt the most feasible and practical measures. In some cases we have good treatments and no practical prophylaxis. In other cases we, have effective prophylaxis and no effective treatments yet known. Prophy- laxis is often impractical in the control of such parasites as many of the nematodes of herbivores, where it is necessary to run these animals on pasture. The animals soil their feet with their feces and the parasites develop too rapidly to make pas- ture rotation at short intervals a practical control measure, owing to economic limitations. Under such conditions treat- ment is more practical and more important than any known prophylaxis. Lack of time and space prevents the abstractive of the ex- cellent addresses presented on the last two days of the meet- ing by Drs. 8S. Sisson, J.-W. Adams, Fred M. Hayes, H. #H. Kingman, O. E. Troy, R. H. Beaudette, F. H. McNair, Fred R. Jones, I. E. Newsom, D. J. Healy, Louis Santa Maria, W. J. Embree, George W. Stiles, Jr., and R. C. Reed, but they will be printed in the JouRNAL as speedily as possible. ELECTION OF OFFICERS The election of officers was the principal feature of the gen- eral session on Tuesday afternoon. Dr. Glover nominated Dr. A. T. Kinsley for President, while Dr. Eliason nominated Dr. T. H. Ferguson. When the ballots were counted Dr. Kinsley had 100 votes against Dr. Ferguson’s 62, so the former was declared duly elected. Other officers eleeted were Charles G. Lamb, Denver, Colo., First Vice-President; J. F. McKenna, Fresno, Calif., Second Vice-President; W. H. Robinson, Portland, Me., Third Vice-President; W. A. Hilliard, Winnipeg, Canada, Fourth Vice-President; Hamlet Moore, New Orleans, La., Fifth Eprroriau 5 Vice-President; M. Jacob, Knoxville, Tenn., Treasurer. The Secretary and also the Editor sueceed themselves. The election of Dr. Kinsley to the presidency creates a va- caney on the Executive Board in the position of member at large. This vacancy may be filled temporarily by the incoming President, but the permanent member must be chosen by the ballots of the members present at the convention next year. ENTERTAINMENT FEATURES Tuesday evening banquets were held by alumni of the vari- ous colleges, as well as by the Shriners’ Club and several other associations. The one big feature of the meeting was the entertainment provided all day Wednesday by the 70-mile automobile drive through Denver’s mountain park system, with a ‘‘beef-steak fry’? at noon on Lookout Mountain, overlooking the town of tolden and adjacent to the last resting place of ‘‘ Buffalo Bill,’’ the Indian scout and buffalo hunter. The return was made by Bear Creek Canyon, one of the most famous scenic motor drives in the Rockies. The wives and families of the delegates were given additional ‘entertainment by an automobile trip through the city and surrounding sections on Thursday afternoon, and a card party Tuesday night. They were likewise present in large numbers at the general session Wednesday evening, when Prof. H. R. Smith, of Chicago, spoke on the subject of tuberculosis eradi- cation and displayed the motion picture ‘‘Out of the Shadows,”’ a film which shows the efforts being made to combat the spread of tuberculosis among cattle. Other films shown included the subjects of hog cholera control, tick eradication and Federal meat inspection. THe LaApres’ AUXILIARY Members of the Ladies’ Auxiliary of the Association met Tuesday afternoon at the Albany Hotel. After prayer by Mrs. C. E. Cotton, of Minneapolis, Mrs. G. H. Glover, of Fort Collins, welcomed the members to the city. The President's address was made by Mrs. A. T. Kinsley, of Kansas City, while Mrs. J. P. Turner, of Washington, D. C., who was unable to be present, sent an excellent paper which was read_ by. the Secretary. 6 EprroriAL ELECTION OF SECTION OFFICERS After the programs of the various sections of the Association had been completed the sections proceeded to the election of their officers, with the following result: Section on General Practicee—W. E. Muldoon, Chairman; H. E. Kingman, Seeretary. Section on Sanitary Science and Police—R. C. Reed, Chair- man; H. Preston Hoskins, Secretary. Section on Education and Research (formerly Section on College Faculties and Examining Boards)—C. P. Fitch, Chair- man; L. W. Goss, Secretary. PLACE OF NExt MEETING Through a real booster’s talk by Mr. Hatfield, of the Chamber of Commerce of St. Louis, an invitation was extended to the Association to hold its next meeting at St. Louis, while both Drs. Eliason and Ferguson solicited the next meeting for Madi- son, Wis. At this session there happened to be only 86 mem- bers present, a slight majority of whom favored recommending to the Executive Board that the latter place be selected. How- ever, the final selection will be left in abeyance until the next ~ meeting of the Board in December. ATTENDANCE AND MEMBERSHIP During the convention there were 337 persons registered, of whom 217 were members of the Association. The number of new members elected at Denver was approximately 250, but this was not considered satisfactory, so a committee on new members was appointed, consisting of the President, the Chairman of the Executive Board, the Secretary and the Editor, to work out some definite plan for increasing our membership. Sugges- tions will be gladly received by this committee. Commissioner of Agriculture Whittlesey of Connecticut was the only honorary member elected this year. Taken as a unit, the convention combined one of the most instructive and entertaining meetings ever held, and a large number of the delegates declared that from both an educational and a social point of view it was of vast benefit to them. - The cool, bracing weather was also deeply appreciated, especially by those who came from sections of the country where the past summer’s heat had been unusually intense. EDITORIAL i OUR NEWLY ELECTED PRESIDENT DR. ALBERT T. KINSLEY, who was elected President of the American Veterinary Medical Association for the ensuing year at the meeting held in Denver, Colorado, September 5 to 9, needs no introduction to the readers of THE JouRNAL. Through his connections with the Kansas State Agricultural College and the Kansas City Veterinary College, his activities in the inter- ests of the veterinary profession and the veterinary organi- zations of which he is a member, his able addresses and liberal contributions to veterinary literature, his ability as a teacher, and his geniality, he enjoys a wide acquaintance among the members of the veterinary profession in this country and is recognized abroad as an authority on veterinary matters. Dr. Kinsley was born of English parents at Independence, lowa, February 26, 1877. His early education was obtained in the public schools of the State where he was born. In 1899 he graduated from the Kansas State Agricultural College with the degree of B. S. Throughout his collegiate course he dis- played such marked aptitude that after graduating he was employed by the College as instructor in bacteriology. He re- mained at the College from 1899 to 1901, where, in connection with his duties as instructor, he continued his studies, and was awarded his M. S. degree in 1901. After leaving the Kansas College he took special work at the University of Chicago, after which he entered the Kansas City Vetermary College, graduat- ing with the class of 1904. Immediately after graduation he accepted the chair of pathology and director of the museum in that college. In addition to teaching bacteriology and pathology from 1904 to 1918, he was President of the Kansas City Vet- erinary College from 1912 to 1918. Dr. Kinsley has been an active member of the A. V. M. A. for many years and was honored with the vice-presidency m 1909-10. He is a member of the Missouri State Veterinary Medi- eal Association and the Missouri Valley Veterinary Medical Association, and served as president of*the latter in 1909-10. In connection with his duties as an edueator, Dr. Kinsley has written many valuable articles, including ‘‘Zine Poisoning in Cattle,’’ ‘‘Wound Healing,’’ ‘‘The Significance of Pathology to the Practitioner,’’ ‘‘Porcine Tuberculocis,’’ ‘‘Ocular Epithelio- Sareoma,’’ ‘‘Epithelioma Contagiosum,’’ ‘‘Equine Infectious Anemia,’’ ‘‘A Disease in Chickens,’’ ‘‘Cornstalk Disease,’’ ete. EDITORIAL DR. A. T. KINSLEY fle is also the author of a work on ‘Veterinary Pathology’? and another on ‘‘Diseases of Swine.’’ Dr. Kinsley is thoroughly fitted for the responsible position im the A. V. M. A. to which he has been elected. He has the backing of the organization, and THE JOURNAL wishes him a ” . a e e successtul administration. THE ADDRESS OF THE PRESIDENT: By Davip 8S. WHITE Columbus, Ohio IT IS REQUIRED by our Constitution that the President deliver an address. This imposes an arduous but not unpleas- ant task. In the political world the President of a country or the Governor of a State delivers, on his installation into office, an inaugural address in which he sets forth, sometimes reiter- ating campaign pledges, his policies. This places him at a de- cided disadvantage, for before he can realize the many situations which will confront him and the many important decisions he must make, he is compelled to commit himself in advance. We have adopted the safer plan requiring that the President’s ad- dress be delivered at the end of his term of office and after he has experienced a year of service. I do not know that the address is ever taken very seriously by the rank and file of the members. I think sometimes it is looked upon as a part of the perfunctory routine, and perhaps by a minority as the ‘‘swan song’’ of the incumbent. To become president of an organization such as this I look upon as a great honor and one which should be conferred only upon a member who through the years has shown his devotion to the profession and to the Association by having given through them some service of benefit to mankind. In looking over the list of my predecessors in office, I believe this has been the aim and object of the membership. I therefore take this occasion to thank each and all of you for this honor. I have tried to accept it in the spirit in which it was given and to render what service ! could to the cause for which we stand. If I have failed it is because I lacked in ability and not in inclination. The term of office is too short for any given President to make an impress upon the organization. According to our Constitu- tion he is elected for one year only and may never hope of reelec- tion. In a democracy, such as our Association should be, this is probably a wise provision. But nevertheless the continuous 1Presented at the fiftyv-eighth annual meeting of the American Veterinary Medical Association, Denver, Colo., September 5-9, 1921. 9 10 Davip S. WHITE administration of the affairs of the Association must be left to other officers whose terms are more permanent. While it is admitted that an absolute despotism is an ideal form of govern- ment, provided a good despot is available, the experience of the world has demonstrated it to be too risky to be adopted gener- ally. Despots are always human and suffer from the frailties DR. DAVID S. WHITE Retiring President, American Veterinary Medical Association ADDRESS OF THE PRESIDENT 1] of human nature, which frailties, as history shows, seemed to be intensified in some despots. Civilized humanity, therefore, has turned from absolute monarchy to constitutional monarchy, and from constitutional monarchy to democracy. While admittedly much has been surrendered to make these changes, the least of the evils seems to be a democratic form of government. I do not mean to advocate that either the term of office of your President be lengthened or his reelection be made possible. I do, however, wish to draw attention to the facts which in part explain the innocuous desuetude into which your President may seem to sink. Fifty-eight years ago a small group of men, graduates of veterinary colleges, met together in an eastern city and founded this Association. They builded better than they knew. In those days veterimary medicine in this country was in a most primitive state. The value of livestock was low and what little veterinary service was required was furnished by the illiterate, self-trained empiric. In no State did laws exist governing the practice of our profession, and with the exception of two or three schools, each offering a most abbreviated curriculum and each dependent entirely upon student fees for its support, there were no oppor- tunities to gain a veterinary education. State veterinary medi- cine did not exist. The only thing that lay before these far- seeing charter members was their faith in the future. During the half century which has followed some of them have lived to witness the changes which time has brought and to realize their aspirations and hopes. Today this Association is the largest veterinary organization in the world. Laws controlling the practice of the profession now exist in practically every State. The private vetermary school which sustained the profession during its formative years has given way to institutions of veteri- nary learning supported by the people. There have also de- veloped National and State Bureaus of Animal Industry, which have as their function the control and eradication of animal disease, now carried on, for the most part, through well planned and directed organizations. The results have justified their existence. In connection with them veterinary research has been continuous and fruitful. Today no countries in the world enjoy greater freedom from animal plagues than do the Dominion of Canada and the United States. 12 Davip S. WHITE One of our longest fought battles against disease was in the nineteenth century when contagious pleuropneumonia of cattle was conquered. One of our most successful campaigns was di- rected against foot-and-mouth disease, which had been an inter- mittent visitor since 1870. In 1914 the most widespread outbreak in the history of the country occurred. Within three months the disease spread over twenty-one States and the District of Columbia. But notwith- standing the extraordinary communicability of the disease and the fact that the rank and file of the profession had had no previous experience with it, and that many people in authority, ignorant of its powers of devastation, opposed our measures of eradication as being too drastic, the disorder was finally com- pelled to desert our shores. With Texas fever there has been a gratifying decrease in the geographical areas infested with the tick harboring the protozoon of this disease. If our well-directed efforts are con- tinued we are justified in assuming that this cattle plague will cease to exist in this country. The importance of this work to the cattle raiser of the Southern States can not be overestimated. It has removed from him the handicap of a restricted market and, permitted him to infuse into his herds the best blood. Dourine, which for a time threatened horse breeding, has also been conquered. We now know how to control hog cholera. The control and eradication of tuberculosis has resolved itself into a purely economic problem. The scientific facts underlying the mechanism of control are now well established. About the accredited herd plan I am optimistic. I believe it will eventu- ally work out, especially since the cooperation of the layman has been secured. In this connection a certain amount of oppo- sition has come from the veterinary practitioner, but I do not anticipate that this will continue when the organizations of official veterinarians have been functioning long enough to learn the part which they should rightly play in the game and the encroachments upon private practice become less obvious. Any scheme of disease control which ignores the reputable prac- titioner is faulty. On the other hand, experience has shown that unsupported individual effort, no matter how skilfully it may be applied, finds the restrictions imposed too great to accomplish the best results. ADDRESS OF THE PRESIDENT il Once we secure the hearty cooperation of the State and National Governments, represented by official veterinarians, the veterinary practitioner and the layman, a successful program of disease control and eradication can be put into effect. Most of the difficulties which we as a profession meet in battling against animal disease are due to misunderstandings, ignorance and sometimes prejudice. However, as right must always prevail in the end, so may we expect that when hearty cooper- ation is obtained among the three groups mentioned, the diffi- culties will be removed and the chaos changed to cosmos. It takes time and energy to develop any piece of smooth-running machinery. The chief engineer of a company building the most expensive automotive vehicle in this country told me that it required six years ‘‘to get the ‘niggers’ out of the motor.’’ It can not be expected, therefore, that in a few months a perfect mechanism of disease control can be designed and put into ef- fectual operation. Disease control means war, and war of necessity makes it unpleasant for many people. The past half century marks the formative period of the vet- erinary profession on this continent. The process of its develop- ment, like that of the other professions, has been. evolutionary. It is still evolving. None of us will ever live to see it perfected. This Association is the only mechanism we have to bring together the leaders of the profession in North America to exchange experiences, witness demonstrations and formulate policies. It has always been my desire to see this Association dominate veterinary policies and politics on this continent. That we have not to date fully accomplished these things has been largely due to our intense interest in mere technical de- tails and the internal politics of the Association. These things are perfectly natural in an organization representing a profes- sion still in its swaddling clothes. Fifty years is a long time in the life of an individual but is only a day in the development of a profession. : The future of this organization will depend upon the leader- ship of those elected to conduct its affairs. Our leaders must be men, therefore, of broad vision and not representative of only one of the various phases of professional activity. It would be a mistake to permit this Association to be dominated by the teacher interested only in teaching, the research man who sees 14 Davip 8S. WHITE nothing beyond his narrow field, the vender of biological pro- ducts imbued only with the spirit of commercialism, the official veterinarian who lays all stress on regulatory measures, or even the veterinary practitioner who cares for naught but the art of practice. Although we can not all be experts at teaching, research, commercialism, officials, or practicers of veterinary art, we can be, every one of us, veterinarians. And of veterinarians only should this organization be made up. Therefore I am ask- ing each group representing a phase of veterinary medicine to go over into the camps of the other groups, learn their prob- lems, see what they are actually up against and endeavor to meet them half way in any act of cooperation which will contribute toward making us a united whole. As the President of the British Veterinary Association puts it: ‘‘No profession, however large, can be really powerful without unity; and in a small profession unity is one of the essentials of existence.’’ We should strive, therefore, to forget our petty differences, trivial | rivalries and little jealousies and become more tolerant of the opinions of our colleagues in order that complete unity of pur- pose and unity of action may be realized. For without these things we can not as a profession bring to bear our full strength, and without this we will not be able to attain our legitimate goal. I can not emphasize too much the importance of unity. While admittedly we have made progress in this regard in the last half century, nevertheless I firmly believe it would have been more rapid and fruitful had we been more firmly united through the years which have passed. Our sister profession, the med- ical, has suffered from the same lack of unity as we. The tendency to split off into cliques and factions has been common to it. Lack of unity and hideboundness has injected into the medical profession various cults and sects which today are sapping the vitality of the physicians belonging to the so-called regular school. The difference of opinion among medical men in technical matters and the tendency for one physician to berate another has had its effect upon the laity. Those without the pale stand nonplused at the situation of the doctors con- tinually disagreeing. Consequently when certain medical cults and sects ask of the laity modifications in the medical law, which gives to them a legal professional status, it is too often . ADDRESS OF THE PRESIDENT 15 granted. In our profession the situation in this regard is much less complex. But we also have our troubles and dissensions although not of the same kind. I think sometimes we are too apt to clique off into little, self- interested groups, each with its own leader and each at cross purposes with the other groups. A strong nation has never been built out of a conglomeration of disunited States, each an autonomous entity and independent of the rest. What would be our history as a nation today had the States Rights doctrine of the Confederacy won over the Nationalism of the Federacy during the Civil War? In place of a mighty republic, now the strongest on earth, we would find this continent occupied by a number of disintegrated sections, each with its own form of government and quarrelling with its neighbors. Firmly united the veterinary profession is already weak enough to fight the battles which it must fight im order to carry on its legitimate work. : Because of certain dissatisfactions, which may be in part justified, there seems to be a tendency among certain groups of veterinarians to abandon already established organizations and initiate ones of their own. This is deplorable. I do not believe that any group of veterinarians representing only one or two of the various phases of the profession can divorce themselves from the rest of us and go it independently. Practice, research, teaching, regulatory work and the military must be ever inter- woven and overlapping. Among them there can be no marked line of cleavage drawn. They are interdependent. One can no more think of removing as a body from the American Veterinary Medical Association or from any State organization a group of veterinarians representing a phase of professional work and expect the mechanism to function any more than one could hope to see a motor car operate from which the transmission had been removed. We must remember the danger of a Mexican political policy, a scheme of self determination run riot, a erippling af- fliction which hurts all of us and in the long run does none of us any good. I have therefore taken as my theme for this message the doc- trine of unity. I have done so in the hope that this acorn of desire and appeal would fall upon fallow ground, where, nour- ished by the soil of harmony and watered by the rain of cooper- ation, it would sprout and grow and develop into a mighty oak 16 Davip S. WHITE whose branches would reach upward to protect against the sun of rancor and discord, and whose every part, even to the small- est twig, would be a component of a beautifully organized whole able to resist the storms of summer and the tempests of winter. Whether it stand in the forest or in the open it represents not only a thing of beauty but also an object of usefulness. To each of the following items I beg leave to briefly draw your attention : Schooils—The greatest need of the profession today, In my opinion, is men with education and the ability of leadership. While I believe we possess a few of this type, as yet not enough have joined our ranks to stabilize our efforts. Our recruiting station is the veterinary school. According to the discipline of the profession each member must be first a graduate of an ac- credited college. Due to influences, some of them emanating from our own ranks, the student population of our schools has suffered a material reduction, especially in the past five years. The following table shows the number of students matriculated in each of the State veterinary schools and the single accredited private school in November, 1920: Alabama-Polytechnic institute Colorado Agricultural College ... £550. ENE i. Sed 88 Geervia State. Agricultural College"... Se ee 21 fowa state -Apricultural College... 2 eee 94 Kansas State ‘Agricultural College... Le eee 61 Michigan Agricultural College... 21 New York State Veterinary College (Cornell) 78 New York State Veterinary College (New York University) ....... 23 MinioOPS Pate MU MiVeEr SUG, ict ecg ha ae See nc 106 Ontario Veterinary College (Toronto, Canada) 0. cccccc ee OD Dnigersivy of ‘Pennsylvania: 0 23 ee FSO Texas Agricultural and Mechanical College 18 State .Collegetot Washington...) 2 SS eee 22 Fadiana Vetermary College..." 3 SS ee 136 J ifs) | en ts. neon re coe 2D It would seem passing strange that in a country of one hun- dred and ten millions of people, with livestock valued at close to ten billions of dollars, only eight hundred and forty-six young men desire to pursue a course in veterinary medicine. There has been a tendency to increase the number of veteri- nary schools supported by the people. As a matter of State pride individual commonwealths have seriously considered the establishment of such institutions. It would be unfortunate ADDRESS OF THE PRESIDENT 17 should the number of veterinary schools increase until there came from the people a greater demand for this type of educa- tion. While there was an excuse for the maintenance of a private veterinary school of mediocre caliber, there can be no reason for the existence of a State veterinary college which is in teaching staff, housing and facilities not in keeping with the increasing demands of animal husbandry. As _ practically atl State-supported schools will be located at land-grant colleges, those having but few students will receive of the legislative ap- propriations granted the institution but a scant share. Medical education is an expensive thing. The cost of turning out a weil trained medical man greatly exceeds that of producing a man of law, divinity, engineering or agriculture. When the distribu- tion of the funds of an institution is on the basis of ‘‘the greatest eood to the greatest number,’’ obviously a poorly attended veter- inary department will suffer financially. It would be far wiser if those States ambitious to support a school of veterinary medi- eine would give only the first two years of the curriculum and in a tangible way assist the student who wishes to complete the course at an already well established veterinary school. This device would not only be more economical for the initial State but would tend to make the best schools more populous in student numbers and better endowed by the States in which they are located. I believe it is the duty of every veterinarian to encourage rather than discourage, as some are doing, young men of the right type to enter the profession. The emoluments of the pro- fession are today greater for the expert than they ever wer, but for the man of mediocre type they often do not offer suf- ficient remuneration to reimburse him for the time and money spent at school, and from now on they never will. There was a day when ‘ a man of no education, meager training and little or no dexter- ‘any fool could farm.’’ There was also a day when ity in the art of veterinary practice vould make a good living or at least a fairly easy livelihood. That day is gone. The Army.—While the situation in the Army is very much better than it was prior to the great war, the Veterinary Service has not yet been given the authority to do its own job nor has it attained the stable status of the other staff corps. The Veteri- nary Corps has still at its head a medieal officer, and I am in- 18 Davip S. WuHiItEe formed that veterinary officers in the field are under the imme- diate supervision of medical officers. Concerning both of these situations I think there exists in the profession a unanimity of opinion. That a veterinary officer should be at the head of the Veterinary Service of the United States Army there seems to be a general agreement. Leading veterinarians of the country, both military and civilian, seem to feel that the absence of such an arrangement places a decided stigma upon a_ profession which in civil life has been able to run its own show whenever it has been given the authority to do so. So much for the diagnosis. The treatment, however, does not seem so simple. The Veteri- nary Service of the Army up until after we entered the great war had been criminally neglected. The older veterinarians, who held the rank of a field officer, had never been given the oppor- tunity to develop themselves ‘as administrators. They were regimental officers, each the technical advisor of his command- ing officer. The idea of an organized service had never been seriously considered even by many of the ranking veterinary officers. However, this is but a repetition of the history of the siaff corps services in our Army, each of which attained its present status only after years of heroic endeavor. It must therefore be expected that the Veterinary Service must pass through practically the same evolutionary stages which other noncombatant services have passed through. Furthermore, 4 military establishment which remains too long at peace tends to degenerate into a state of conservatism rarely approached in civil life. This conservative mental attitude harbored by many officers of high rank and influence obviously make progress for the new-created veterinary service extremely difficult. The 3ritish Army, which gives to its veterinary officers greater au- thority than any army in existence, and which for this reason has been able to create the most efficient animal salvage corps the world has ever known, offers from a veterinary standpoint many advantages over our own. In the first place it made the veteri- nary service attractive to young men not only in rank and pay but in opportunity to see the world and some action, as this army is fighting somewhere nearly all the time. It took Great Britain, however, nearly half a century and two great wars to create its army veterinary service. It will probably require of us as long a time. ADDRESS OF THE PRESIDENT 19 I have been in correspondence with Major General Ireland, Surgeon General of the Army, in regard to this matter, and have offered some suggestions which im time should work out to the benefit of the Veterinary Service. In my opinion General Ire- land thoroughly understands the situation, is in sympathy with the veterinary officer and his problems, and will eventually be able to set the Veterinary Service on its feet. It would seem to me logical, inasmuch as no veterinary officer of sufficient rank is available to act as director, for the Surgeon General to select from among the veterinary officers one who with opportunity and experience would make an efficient director and who event- ually could be placed at the head of the Veterinary Corps. If something of this sort is not done, I do not see how we can hope to develop a veterinary officer for this position. I do not believe any attempt has been made to do this thing but I do feel the present incumbent should have an understudy, a veterinary officer who could take his place when he is relieved. I have the highest regard for Colonel Morse. He is a good administrator, a broad-minded gentleman, and has proved an excellent ‘‘ wet nurse’’ for the veterinary baby. However, it is to be hoped that this youngster will soon be weaned and taught to eat solid food. In my opinion it will be some time before the Veterinary Corps can become a separate organization. To attempt to make it one at this time would be disastrous. For the present and until it has worked out its salvation and attained an undisputed status in the Army it should remain in the Medical Department. For many reasons it is better off there than it would be in any other department. National Research Council—Pursuant to the action of this body, a member of it, a veterinarian of national reputation in research and experiment, has been appointed a member of the National Research Council. I believe this to be a good thing. The more we mingle with scientific, folk outside the profession the better will the profession be recognized and esteemed by these folk. Furthermore, many problems in medicine of direct interest and usefulness to the human being can be best worked out through cooperation with the properly equipped and trained veterinarian. National Narcotic Law.—A communication has been received m0) Davip S. WHITE from the National Committee, representing the professions of medicine, dentistry and pharmacy, at work drafting a new na- tional narcotic law, requesting that this organization also be represented. It would seem fitting, therefore, for us to take action in order that our rights and privileges be looked after when this matter is being given consideration. General Anesthesia Law.—The Blue Cross Society has re- quested that this Association use its good offices to create a sentiment which will lead eventually to the passage of State laws requiring that general anesthesia be employed in all major surgical operations upon animals. For practical reasons the castration of animals is excepted. A similar law has been enacted in England. Such legislation, it is thought by the Society, would, among other things, tend to limit the number of major surgical operations performed by empirics. Permanent Secretary.—l|t has been voted to authorize the Executive Board to make the office of Secretary more perma- nent, probably combining with it the editorship and management of the JouRNAL of the Association. This matter has been given careful consideration by the Executive Board, whose report will vive the results of their deliberations regarding it. United States Meat Inspection Service.—Influences are at work to transfer the Meat Inspection Division of the Bureau of Ani- mal Industry to a Department of Public Welfare. The transfer is urged on the grounds of economy and efficiency. As it looks at this distance, there lurks within the danger that national meat inspection would be taken out of the hands of the veterinarian and placed under the supervision of a physician. The transfer would also divorce from the Bureau its largest division and very probably would lead to expensive duplication in laboratory facilities and equipment. In the light of our present informa- tion, the step seems inadvisable both from the professional and lay standpoints. It is certainly the duty of this Association to investigate thoroughly this important matter, and to do so before the transfer is made, in order to be in a position to prevent it if it is to work injury to the profession. This is a national problem entirely within the province of this Association to consider. It is hoped that you will enjoy a successful meeting both from the standpoints of acquiring information and at the same time ADDRESS OF THE PRESIDENT 21 most healthful recreation. You are being entertained in Amer- ica’s great playground, the mountains of Colorado. You are among the biggest hearted, most whole souled and hospitable people in the world—the people of the Great West. I trust, therefore, you may not only gain from the literary programs arranged for you but may also enjoy yourselves to the utmost as guests of the profession of this great State. THE HORSE FOR STREET CLEANING Director Frank H. Caven of the Philadelphia Department of Public Works has advanced ideas on the subject of street cleaning and rubbish collection. His plans for the erection of destructories for the odorless cremation of street refuse is evidence of this fact. This is the electric age—the ‘‘Johnny-on-the-spot’’ era of ef- ficiency. It is the period when efficiency engineers flourish and by- products are the creators of greater wealth than the original article of production. Notwithstanding this the experimental conclusion of the Di- rector and his force of engineers is that it is greater economy to spend something like $800,000 for trucks, wagons and horses with which to start a city-wide street-cleaning program than to put the same amount of money into costly gasoline trucks, dump carts and auto machinery. A tremendous upkeep is the principal argument against motor vehicles in street cleaning. With the construction of destructories long hauls necessitated by far-distant dumps will be eliminated. A team of horses for short hauls is more economical than the electric vehicle, at least at the present stage of development in street cleaning. About 1,400 horses are required to keep the city streets clean. This includes teams for the collection of rubbish and garbage. These horses are used to move ‘21 flushers, 57 trucks, 515 wagons, 100 machine brooms and 50 sprinklers. If the Department of Public Works went into the auto ash collection scheme over 1,000 motor-driven vehicles would be re- quired to keep the city clean. GARBAGE FEEDING IN RELATION TO THE CONTROL OF HOG CHOLERA * By FREDERICK TORRANCE Veterinary Director General, Canada GARBAGE as a source of hog cholera had been under sus- picion for some time previous to the year 1912, when Dr. C. D. MecGilvray read a paper before this association on the sub- ject. He gave particulars of several outbreaks in the Province of Manitoba, where every other source of possible infection could be excluded, and only garbage remained as the prob- able infective agent. The presence in the garbage of un- cooked seraps of pork which might have come from hogs affected with incipient hog cholera was suggested by Dr. Mc- Gilvray as the cause. The statement that hog cholera might be conveyed in this way was received with some incredulity at the time, but ex- perience and experiment have confirmed the result which he reached by a logical inference. The Health of Animals Branch of the Department of Agri- culture, dealing with hog cholera as it made its appearance from time to time in various parts of Canada, accumulated a lot of evidence that garbage feeding was a very real source of danger and should be controlled if possible. A new regula- tion was therefore made and became law on April 27, 1915. It is as follows: “The feeding of swine upon garbage or swill, either raw or cooked, obtained elsewhere than on the premises where fed, is prohibited, unless special permission in writing is first obtained from the Veterinary Director General.’’ Feeders of garbage were notified to make application for a license and required to sign an agreement as follows: ‘‘In consideration of the granting of a license to me, I here- by agree (1) to boil thoroughly all garbage before feeding it to swine, and to prevent my swine from having access to uncooked garbage; (2) to maintain my hogs in a clean, sani- tary condition; (3) to sell no hogs except for immediate 1Presented at the fifty-eighth annual meeting of the American Veterinary Medical Association, Denver, Colo., September 5-9, 1921. 22 GARBAGE FEEDING AND HoG CHOLERA oo slaughter; and (4) to notify the veterinary inspector without delay if sickness appears among my hogs.’’ On receipt of a signed application an inspector is detailed to visit and report on the premises, and no lhcense is issued unless the premises are fairly such as can be kept clean and sanitary and are provided with the appliances necessary for cooking the garbage. For those keeping only a few pigs, up to twelve but not more, an ordinary food boiler was con- sidered satisfactory. For owners having any number in excess of twelve we require a steam boiler and vats of suf- ficient capacity for the size of the herd. Inspection of licensed garbage feeders is carried out by a staff of lay inspectors who visit the premises from time to time and report on the conditions as they find them. If a report shows that a garbage feeder is neglecting to cook the garbage, or failing to boil it sufficiently, or is keep- ing his premises in a dirty, insanitary condition, he is given a warning, and if conditions are not made satisfactory at once his license is cancelled. The inspectors are constantly on the lookout for persons feeding garbage without license, and either warn them to procure a license without delay or else action is taken to have them brought before a magistrate and a fine imposed. In case a lay inspector should find some hogs sick he im- mediately reports to a veterinary inspector, who visits the cases, makes a diagnosis and deals with the outbreak as cir- cumstances require. If the disease is hog cholera, the affected hogs are slaughtered, the hogs in contact are serum treated and the premises placed under quarantine. The hogs on neigh- boring premises are visited and if deemed necessary by the inspector they too are serum treated and the premises quaran- tined. In Canada we pay compensation for hogs slaughtered by order of an inspector for the control of a contagious disease. Compensation is limited to two-thirds of the valuation, which must not exceed $20 for a grade or $75 for a purebred pig. When hog cholera breaks out on a licensed garbage feeder’s premises it is assumed that he has failed in his duty to cook the garbage properly or to prevent hogs from gaining access to it before it is cooked. Compensation is therefore with- 24 FREDERICK TORRANCE held unless it can be shown that the infection came from some other source than uncooked garbage. This system has now been in operation for over five years and is found to work well. It has not altogether wiped out hog cholera, but it has enabled us to get early information of outbreaks and by dealing with them promptly to prevent their extension to adjoining premises. The frequent inspec- tion and the insistence on sanitation have had a marked effeet in improving the cleanliness and comfort of the swine kept on licensed premises and have helped to remove what was often a nuisance in the outskirts of a city. A comparative statement of five years before and five years after this system was adopted will indicate the progress made. The first period is from April 1, 1909, to March 31, 1915; the second from April 1, 1915, to March 31, 1921: First period. Second period. Total number of outbreaks............... 1,426 742 Total number of swine slaugh- (S612/0 Pe een ae: Sone Nena Cn nt ined 42.668 19.709 Valuation of swine slaughtered... $368,783.81 $247 901.70 Compensation paid for swine SH APETU ICA IW ONL @ laa Sen, SEER ia ea a etd 245,455.72 141,545.43 It will be noticed that the number of outbreaks has been practically cut in half. Compensation would have been cor- respondingly reduced but that during the first period the maximum valuation was #15 and $50 as against $20 and $75. This increase was made in consequence of the claims of live- stock owners that the higher market prices of cattle and hogs entitled them to a higher rate of compensation. The improvement in the hog cholera situation since the licensing of garbage feeders was begun is better shown perhaps by the figures for each of the five years: Number of Com- Near. 7% Outbreaks. hogs killed. pensation. ci st Lies ee a rr 290 2,700 $33,699.95 RRMA Ae Reheat eB 8 Tore... 90 4.623 30,497.59 1 OMS ame memes ie ks, 62 2212 13.031.20 TD ec a 52 2.163 23,342.74 LODO eee ee 92 1,642 19,001.71 1921 (4 months')s.2...... D 81 198.00 Ina country as large as Canada these results have not been attained without exciting opposition and sometimes active an- GARBAGE Frepinc AND Hog CHOLERA 23 tagonism. The class of persons engaged in this occupation is not noted for intelligence. Some of them are foreigners to whom our laws are strange. Our inspectors have sometimes been met with threats and even physical violence. We have persisted, however, and have not hesitated to invoke the law whenever less drastic methods failed. During the period covered by this paper we have instituted sixty-three prosecu- tions and secured fifty-nine convictions, chiefly for the offense of feeding collected garbage without a license. A final word as to the connection between garbage feeding and hog cholera. We have records of 742 outbreaks of hog cholera of which all but 75 were attributable to this cause. In other words, 90 per cent of the hog cholera we have had in Canada during the past five years had its origin in the garbage pail. Our system is working well. We now have 471 licensed garbage feeders and so far as I know not a single case of hog cholera in the whole of Canada. JAPANESE RULES OF THE ROAD (Promulgated in Tokio) 1. At the rise cf the hand policeman stop rapidly. 2. Do not pass him by or otherwise disrespect him. 3. When a passenger of the foot hove in sight, tootle the horn trumpet at him, melodiously at first, but if he still obstacles your passage tootle him with vigour and express by word ot mouth the warning, ‘‘Hi, Hi.’’ 4. Beware the wandering horse that he shall not take fright as you pass him. Do not explode an exhaust box at him. Go soothingly by. 5. Give big space to the festive dog that shall sport in the roadway. 6. Avoid entanglement of dog with your wheel spokes. 7. Go soothingly on the grease as there lurk the skid demon. 8. Press the brake of the feet as you roll round the corners to save the collapse and tie-up.—Journal of the American Medical Association. RESULTS FROM IMMUNIZING CATTLE AGAINST ABORTION * By F. B. Haney University of Wisconsin, Madison THE one question about bovine contagious abortion that livestock men and veterinarians are asking more often than any other today is in regard to methods of establishing im- munity against the disease. Although there is yet much to be learned about the subject, it is certain that the experimen- tal work which has been conducted to shed light on the prac- ticability of establishing an artificial type of immunity has demonstrated the possibility of this means of control. Since extensive, adequately controlled experiments have been conducted in England, Germany and Wisconsin with several different immunizing products, each of which has given some- what different results in the hands of the various investi- gators, it should be interesting to compare the results, as the countries in which the work was done are widely separated. In this way it seems that the controversy that exists relative to the efficiency of these products as aids in preventing con- tagious abortion may, in a measure at least, be settled. Three distinct biologic products have been employed in these experiments. These have been used alone as well as in various combinations. They are (1) a bacterin consisting of dead abortion bacilli; (2) a vaccine consisting of living abortion bacili; (3) an immune serum produced by giving cows large and repeated doses of abortion bacilli, then bleeding them and recovering the blood serum. It is significent that the product containing the dead abor- tion bacilli is recommended almost exclusively by commercial firms who are its chief exploiters. Some of these firms claim astonishing results from the use of abortion bacteri, but have no reliable data to substantiate the claim. Certain manu- facturers go so far as to guarantee certain results from the use of their abortion bacterin. Claims of this kind must be great exaggerations, if results reported in this paper are authentic. i1Presented at the fifty-eighth annual meeting of the American Veterinary Medical Association, Denver, Colo., September 5-9, 1921. 26 ba | IMMUNIZING CarrTLeE AGAINST ABORTION my It is charitable to believe that the exaggerated claims have been based on the fact that bacterins have given such excellent results in typhoid fever of man, rather than upon their actual use in veterinary practice as an immunizing agent against contagious abortion. The abortion vaccine has been used for creating immunity against abortion on the strength of favorable results secured | from the use of a similar product in certain other infectious diseases of animals. Theoretically it should be capable of stimu- lating the production of abortion antibodies in sufficient amounts to prevent any abortion bacilli, that may subsequently gain entrance to the body, from producing disease. The immune serum has been used alone to a limited extent only, as trials showed it to be of little value. It has been em- ployed simultaneously with both the dead and living abortion bacilli; also to sensitize the live organisms for the purpose: of preparing the as yet little used products known as abor- tion serovaccine and serobacterin. In Table 1 is given a summary of the results of an investi- gation conducted by veterinarians for the Board of Agricul- ture of England in an attempt to prove or disprove the value of two of the products under diseussion. The herds pre- sented had all experienced severe losses from abortion just prior to treatment. TABLE 1.—ReEsuuts oF ImMMuNIzING CATTLE AGAINST ABORTION IN ENGLAND ; Total Less those Per Number | Per cent Method of conferring number which died, Number} cent calved* | calved immunity of were barren aborted | aborted | correctly | correctly animals or were sold Immunized with live bacilli... . . 594 594—101=493 F32 461 93.5 | 6.5 Controls not immunized........ 472 472— 40=432 101 | 23:4 331 76.6 | 21.0 87 79.0 | Immunized with dead bacilli... . 146 146— 36=110 23 The following conclusions seem justified from the data pre- sented in Table 1: (1) That immunization with live abortion ba- eilli (vaccination) resulted in conferring immunity on 93.5 per cent of the cattle treated in herds where over 30 per cent of them aborted before the experiments began. (2) That over 25 per cent of the untreated controls aborted. (3) That treatment with the dead bacilli conferred little if any immunity, for the percentage of abortions in this group was practically the same as in the group left as controls. This means that al- 28 EF. B. Hapiry though the abortion bacterin caused little or no harm, it did not prevent the occurrence of abortion with any degree of regularity or to any extent. The men in charge of this series of experiments came to the conclusion that: (1) It is necessary to continue the imocula- tions for a time on infected premises after abortions cease ~ to oceur; (2) inoculations should be carried out in an infected herd until abortion has ceased, but if the herd is one which is restocked by buying in fresh non-pregnant animals, these ani- mals should always be inoculated; (3) if pregnant animals are bought, they may reintroduce infection; (4) although very few animals which have been immunized for one preg- naney have aborted at subsequent pregnancies, it appears that if an animal is immunized for two pregnancies running, there is very lttle chance of the animal aborting afterwards. German investigators have also conducted immunization ex- periments against contagious abortion on a relatively large scale. The results of this work are detailed in the Arbiter aus dem Reichgesundheitsamte, 52 Band, Heft 3, in the following manner : Tasie 2.—Resuuts oF ImMmMuNiIziING CarTrLe AGAINST ABORTION IN GERMANY Immunizing material Number | Number aborted | Per cent Per cent calved employed | treated | after treatment aborted normally | Dead bacilli Se a eee - 937 117 > 12.5 87.5 Dead bacilli plus immune serum | 157 | 20 ETT 87.3 Live nbactllienpe se ee ae | 482 28 Ro 5.8 94.2 Live bacilli plus immune serum | 57 3 ea 94.7 Inimune serum soe see es 17 9 ) Sad) A 47.0 Controls not immunized........| 1,356 245 ee oral 51.9 The conclusions arrived at by the German investigators from the results of their observations were as follows: (1) The dead bacilli in both non-pregnant and pregnant cows pro- duced some degree of immunity; it was, however, of low de- gree and of short duration. Many cows did not abort at the first calving after this method of treatment, but did at the second. (2) The dead bacilli when injected with immune serum vave results similar to those obtained from the use of the dead bacilli alone, and lead to the conclusion that both are of little use. (3) The live bacilli gave better results in all circumstances than the dead. Of 128 cows, which had previously aborted, treated with live bacilli, only six aborted afterwards. Better results were also obtained in preventing abortion in cows that IMMUNIZING CatrrLe AGAINST ABORTION 29 had never aborted yet were in herds with infected cattle. (4) The live bacilli plus the immune serum gave about the same protection as the live bacilli alone. This method also proved helpful in preventing abortion in pregnant cows, reducing the losses among them as did the live bacilli alone in non- pregnant cows. (5) The immune serum alone was found to be practically worthless. (6) The controls were injected with plain broth which gave no protection; in fact, there were more abortions in this group of cows after treatment than before. It was observed that widely varying results occurred in different herds. For example, in some herds abortion dis- appeared completely; in others there was a decrease; in still others there seemed to be no change either way; while in a few herds there was an actual increase. As a result of all the methods of immunization taken together, there was a net de- crease in abortion. During the two-year period between January 1, 1919, and December 31, 1920, the Department of Veterinary Science of the University of Wisconsin prepared and distributed through veterinarians in Wisconsin nearly 1,000 doses of this vaccine. At the time this is written reports have been received on 474 vaccinated cattle and 101 controls in 42 different herds. These cattle were kept under ordinary farm conditions, so the criti- cism that the animals and their quarters were not typical can not be made. The vaccine used in this Wisconsin experi- ment was prepared very carefully to insure a uniform, un- contaminated and fresh product. In order to find out how eattle of different ages and classes respond to the vaccine treatment, the animals were elassified or divided into the various groups shown in Table 3. So far as was possible some animals in each herd were left unvacei- nated as controls. It should be understood in studying these data that the disease was present in the majority of the herds here represented and had caused heavy losses in some ot them, but it is not possible to give either the herd or the in- dividual history. The average abortion rate in these herds previous to treatment was not determined. The controls had an average abortion rate of 31.2 per cent during the two- year period mentioned. A considerable number of the virgin 30 F. B. HapLEY Tasie 3.—Resutts or VaccinatinG Carrte AGaryst ABortion IN WisCONSIN (The 42 herds here represented were under observation from January 1, 1919, to December 31, 1920.) | Total | Description number | Less those | Number) Per Number | Per cent of animals of { barren, aborted cent calved calved animals sold, died aborted | normally | normally Unbred heifers vaccinated . 136 | 136— 9=127 Does PX ai 95 77.9 Unbred heifers left unvac- cinated as controls...... lk Phag 26— 2= 241 8 33.3 16 66.7 Open cows that have not | | _ aborted, vaccinated... .. 221 221—14=207 17 8.2 190 91.8 Open cows that have not | ‘ aborted, left unvacci- | nated as controls. ......| 21 23 — ls 10 55.6 8 44.4 Open cows that have ; aborted, vaccinated... . . TB || 73— 3= 70 11 ert: 59 84.3 Open cows that have | aborted, left unvacci- | nated as controls...... .| Closed cows that have not | | aborted, vaccinated.... . 13 TS sale) 3 24 i 8 12. Closed cows that have not | aborted, left unvacci- nated as controls. ...... 26 26— 4= 22 ~] vu ho to al = -1 “ Closed cows that have aborted, vaccinated... .. 31 31— T= 24! 3 12.0 Pei 84.0 Closed cows that have | | aborted, left unvacci- | nated as controls....... 3 | 3— l= 2 1 50.0 | 1 50.0 Entire number vaccinated .| 474 | 474—35= 439) 62 14.1 397 85.9 Entire number of controls . | 101 | 101—11= 90) 28 BY 62 68.8 | | | | | | heifers had never been exposed to infection before beimg vaccinated. Analysis of the figures given in Table 3 shows that out of 127 unbred heifers that were vaccinated previous to concep- tion 77.9 per cent ealved normally, while only 66.7 per cent of the controls did. In other words, the abortion’ rate in the controls was 33.3 per cent, which is very high and shows that the infection which existed in the herds was virulent. These results from the use of the vaccine in heifers. were not so satisfactory as we had anticipated for this age group before tabulating our data, but they do demonstrate that the vac- cine has immunizing value for heifers. The figures support the well-known belief that heifers are more susceptible to the abortion disease than are mature cows. They do not coincide with the opinion of the German workers that age has no bearing either on susceptibility to the disease or to immuni- zation, provided that the animals are sexually mature. The most gratifying results were obtained with open cows that had never aborted. In this group the vaccine was 91.8 per cent effective, which is in marked contrast to 44.4 per [IMMUNIZING CarrLeE AGAINST ABORTION . 3] cent of normal calvines for the controls. This indicates that the vaccine has its greatest value for open cows in herds where the epizootic is known to exist. The vaccine had little value when administered to open cows that had aborted. In this group the percentage of cows that calved normally was nearly the same as in the case of controls. This is just what one would expect when the fact is taken into consideration that some immunity is conferred as a result of a naturally acquired infection. PER CENT THAT ABORTED UNBRED HEIFERS VACCINATED YU) 4G 0:3:30:0- UNBRED HEIFERS LEFT UNVACCINATED AS CONTROLS 8227 OPEN COWS THAT HAVE NOT ABORTED VACCINATED WLM UY IA NER SNNACEINATED AS CONTROLS. OPEN COWS THAT HAVE ABORTED VACCINATED % OPEN COWS THAT HAVE ABORTED LEFT Wh. ZY UNVACCINATED AS CONTROLS CLOSED COWS THAT HAVE NOT ABORTED VACCINATED IZA, SED SOME TAS CSRUERNOT ABORTED LET CLOSED COWS THAT HAVE ABORTED VACCINATED CLES ON PEE ANS EE EEE ENTIRE NUMBER VACCINATED WY N22, ZZ, ENTIRE NUMBER OF CONTROLS Comparison of Abortion in Vaccinated and Unvaccinated Cattle in ‘Wisconsin Experiments. (See Table 3 for Details.) As would be surmised, a higher percentage of the cows that Were pregnant at the time of vaccination and that had never aborted did abort subsequently than did the controls that Were not vaccinated. The difference, however, was not marked. Of the cows that had aborted and that were pregnant at time of treatment, the figures show that the percentage of nor- mal calvings was nearly the same as in aborters that were vaccinated before being bred. The number of controls in this group is so small that no conclusions can be drawn from them. KF. B. Happry Ww Altogether 474 cows and heifers were vaccinated. Of these 439 were available for data, the others having failed to breed, been sold or died. Only 14.1 per cent of these aborted, while of the 101 controls that were not treated 31.2 per cent aborted. When these figures are compared it is séen that the abortion rate was twice as great in the controls as in the vaccinated animals, The writer believes that these Wisconsin experiments war- rant the conclusion that the abortion vaccine has a decided immunizing value, especially for cattle of certain groups. The vaccinated cattle showed a decrease in both the abortion rate and the sterility rate. As a result there was an increase in the breeding efficiency of the treated over the untreated ani- mals. This was particularly marked in the second gestation period. In some herds no abortions occurred subsequent to the treatment; in others the abortion rate was not changed; in a very few the treatment actually seemed to be a detri- ment. The sequele of contagious abortion infection in many herds were favorably influenced; for example, in some herds, where repeated services had been necessary before conception occurred, the cows after inoculation promptly conceived; there was also less trouble reported from retained placenta. Let us now compare the results secured in England, Ger- many and Wisconsin from the use of the dead abortion baeilli, or that product known commercially as abortion bacterin. In England it was found to be of no value whatsoever; in Ger- many it produced some degree of immunity, but-the protee- tion was of short duration, and not substantial enough to be depended upon; in Wisconsin this product was used in a series of experiments, not reported in this paper, in which it was found to have absolutely no immunizing value. These data warrant the conclusion that under both farm and experimental conditions abortion bacterin has failed. By comparing the results from the use of the live abortion bacilli as shown in Tables 1, 2 and 3 it is seen that this product was 93.5 per cent effective in preventing abortion in England, 94.2 per cent effective in Germany and 85.9 per cent in Wisconsin. This is an average of 91.2 per cent, which is very gratifying. In England 23.4 per cent of the control animals aborted, 18.1 per cent in Germany and 31.2 per cent IMMUNIZING CaTTLeE AGAINst ABORTION 33 in Wisconsin, which makes a general average of 24.2 per cent. The difference in results may be accounted for by the differ- ence in virulence of the infection, which apparently was most pronounced in Wisconsin, considerably less pronounced in England, and markedly less in Germany, as fewer untreated controls aborted in the latter two countries and a smaller percentage of abortions occurred among the vaccinated ani- mals in these foreign countries than Wisconsin. The possi- bility also exists that the Wisconsin cattle were more sus- ceptible to infection. In closing, the writer wishes to warn against the dangers of passing premature judgment on the value of any thera- peutic agent for contagious abortion. One should guard him- self against excessive optimism based on the results of limited trials, for this might be a means of confusing the issue. There- fore the results reported in this paper should be taken with the same degree of conservatism in which they are given. In view, however, that success was achieved in such a large number of animals on which the statistical records are com- plete, the idea of attributing the results to spontaneous cure may be dismissed. A shipment of 3,000 reindeer carcasses, to be sent to all parts of the United States, including the Atlantic seaboard, was due to arrive in Seattle from Nome September 1. Five thousand rein- deer are available for meat, but transportation facilities will per- mit only 3,000 to be shipped. The surplus young male reindeer are now being culled out preparatory to slaughtering and ship- ping. All fresh meat will be stored in the refrigerators of the Seattle Port Commission until redistributed. One of the startling conclusions to be drawn from poultry culling work in Iowa the present season is the widespread prev- alence of disease found in farm flocks by the investigators. Some reports show that as high as 75 per cent of the flocks in some counties are found to be tubereculous.. The general results found are indicative of the fact that disease is much more prev- alent than it was last year.—lowa Homestead. THE RELATION OF THE CORPUS LUTEUM TO ESTRUM AND INVOLUTION OF THE PATHOLOGIC UTERUS: By W. lL. Boyp University Farm, St. Paul, Minn. IN discussing the corpus luteum the writer thought it ad- visable to give a brief discussion of the histology and physi- ology of this endocrine gland. The corpus luteum or yellow body is a gland of internal secretion and originates or develops from the mature Graafian follicle following its rupture. Immediately beneath the modi- tied mesothelial covering of the ovary is the germinal epi- theium. The germinal epithelium proliferates actively, as do also the primordial ova, and is said to dip or extend down into the stroma, carrying with it the ova. These groups of cells are called Pfluger’s tubes. The connective tissue sur- rounding these columns of epithelial cells develops in such a manner that masses of germinal epithelium containing one or more primordial ova are split off, until finally the ovary is formed entirely of primary follicles and connective tissue stroma. The Graafian follicles, which were first described by Rene de Graaf and named after him, are found in three different stages: primary, growing and maturing follicles. As the follicle or ovisac approaches maturity, it is then located near the surface, where it finally projects to the exterior in the form of a vesicle. When the follicle is fully matured the fibers of the theca separate, necrosis results, and the follicle ruptures at this point, which is known as the stigma. The rupture of the ripe ovisac with the discharge of the ripe ovum consti- tutes the act of ovulation. The wound, if one may be permitted to eall it such, which results from ovulation, rapidly fills with blood, forming what is known as the corpus hemorrhagicum. This body is soon re- placed by large epithelial cells, which, according to Novak, 1Presented at the twenty-third semi-annual meeting of the Minnesota State Veterinary Medical Association, Hutchinson, Minn., July 14 and 15, 1921. Published with Journal Series No. 268. 34 Corpus LurEeEUM AND EsStTRUM oD originate from the stratum granulosum. These cells contain a yellowish brown pigment first called lutein, but now ace- cording to Escher is known to be carotin, a substance identi- cal with the pigment of carrots. This pigment is very pro- nounced in the cow. The corpus luteum of the mare, ewe and sow is not so highly colored, and according to Palmer carotin is not present in the lutein cells of the sow. The corpus luteum of pregnancy develops to about the size of a marble or an acorn and occupies almost the entire part of the ovary, being surrounded by a network of capillaries. The arrangement of the cells approximates the placement of the cells in the cortical region of the suprarenal gland. The false corpus luteum does not grow to any great size and as a rule practically disappears within twenty to thirty days. The histological appearance of both true and false corpora lutea is probably identical. THE FUNCTION OF THE CorPUS LUTEUM To Gustav Born of Breslau belongs the credit of being first to suggest that the corpus luteum is a ductless gland produc- ing a secretion which prepares the endometrium for the at- tachment of the fertilized ovum. Frankel’s researches on this subject have been extensive and his general conclusions are as follows: The corpus luteum is a ductless gland which is renewed every four weeks during reproductive life in the human female and at different intervals in the various mammals. . . . Its function is to control the function of the uterus from puberty to the menopause, to prevent it from lapsing into the infantile condition or undergoing atrophy, and to prepare its mucous membrane for the maintenance of the ovum. If the ovum be fertilized the corpus luteum is responsible for maintaining the raised nutrition of the uterus during gestation. If the ovum be unfertilized it merely produces the hyperemia of men- struation and then undergoes degeneration until it is renewed in a fresh position. Most observers are agreed (with a few exceptions) that estrum and menstruation do not occur after the ovaries have been extirpated, and that the phenomenon of menstruation is brought ~ about by an internal secretion from the ovary. As stated above, Frankel believes this secretion is supplied by the corpus luteum. But Marshall contradicts this by his statement that in most mammals ovulation does not oceur until estrum. He mentions that in the rabbit ovulation may not take place until stimulated 36 W. L. Boyp by coition, and in the dog ovulation occurs after external bleed- ing has been in progress for several days. Marshall is of the opinion that in our domestic animals the act of menstruation precedes estrum: In relation to the bovine, our observations are different from those recorded by Marshall. We have noticed that cows frequently menstruate, not markedly but noticeably so, two to three days following estrum. We also believe that in most cases where cows are bred and fertilization takes place, menstruation does not occur. If the ovum remains unfertilized, or if the fertilized ovum is aborted, menstruation usually fol- lows. We have, however, known of individual animals to have menstruated after service, yet having been successfully fertilized. Most herdsmen are now cognizant of the fact that cows or heifers, particularly heifers, which menstruate following service will again be in estrum at the termination of the next dioestral period. Marshall and Jolly state that corpora lutea are not present during the proestrum, and are therefore functional only subse- quent to ovulation. We believe that generally speaking this state- ment is true, but when applied. to the bovine it should be modi- fied. We have upon numerous occasions found cows to be in estrum in which a corpus luteum was present. In relation to the function of the corpus luteum and the cau- sation of estrum, Marshall and Jolly conclude as follows: The ovary is an organ providing an internal secretion which is elaborated by the follicular epithelial cells or by the interstitial cells of the stroma. This secretion circulating in the blood induces men- struation and heat. After ovulation, which occurs during estrus, the corpus luteum is formed, and this organ provides a further secretion whose function is essential for the changes taking place during the attachment and development of the embryo in the first stages of pregnancy. It is our belief that the corpus luteum bears a much closer relationship to estrum than the researches of Marshall and Jolly would indicate. Particularly is this true in the cow. The corpus luteum in the cow probably does not undergo as rapid degenerative changes as it does in other domesticated animals. This may be the explanation of the fact that mares come in heat following parturition much earlier than do cows. In eases (with few exceptions) where the corpus luteum. fails to degen- erate or become absorbed it interferes with estrum. This kind of a structure has been termed a persistent corpus luteum, In Corpus LureuM AND EstrRuUM 37 non-pregnant cows in which there is an absence of estrum, and in which there is a corpus luteum present without other exten- sive pathologic conditions, the removal of same is with marked and astonishing regularity followed by estrum within three to five days. The corpus luteum undoubtedly interferes with ovu- lation mechanically and by the elaboration of an internal secre- tion which exerts an inhibitory action on the maturation of the Graafian follicles. We have examined a large number of cows in our clinic for the purpose of determining the reason or reasons for the ab- sence of estrum. Many of these animals, in so far as we were able to judge from a physical examination, had (with the ex- ception of a persistent corpus luteum) normal reproductive organs. The corpus luteum was removed, or, if difficult of removal, was massaged for the purpose of making its enucleation more easy at the next examination, which has usually been at inter- vals of ten days to two weeks. Invariably estrum would follow within three to five days after operating. There were, however, exceptions to this rule, as some cows came in heat as early as two days after being operated on, while others required as long as ten days. In some cows estrum undoubtedly did take place, but went unnoticed, and for this reason it was thought that the removal of the corpus luteum did not produce the well-known signs of heat. Examinations for such cases will as a rule reveal the presence of a newly formed corpus luteum. We have oper- ated on cows where there was a persistent corpus luteum in the left ovary. Estrum took place within a few days when they were bred and then watched carefully for the presence of the next estrum. If heat did not occur they would be returned to the clinic again within forty to sixty days, the length of time being determined by the age of the animal. At this time an examina- tion for pregnancy was made, and in those which proved to be pregnant we would frequently find a right horn pregnancy, with the corpus luteum of course located in the right ovary. In eases where the persistent yellow body was removed from the right ovary, the above described changes were frequently re- versed. This sort of evidence supports the theory that the corpus luteum does interfere mechanically with ovulation in the ovary in which it is located, and probably by some internal se- 38 W. Le Boyp eretion which inhibits ovulation in the opposite ovary. Where the corpus luteum has undergone a certain amount of atrophy and has become deeply situated within the ovary it is often necessary to practice ovarian massage for a number of weeks before it can be safely dislodged. In one or two cows we have had to resort to ovariectomy, and in each instance estrum ap- peared as soon as healing was complete. The removal of the corpus luteum in the early stages of gesta- tion is followed by the expulsion of the embryo or fetus within a few days. If a small portion of the lutein tissue remains, regeneration may take place to such an extent that it may prevent abortion. Heifers that have been bred too young, or in cases where purebred heifers are in calf to scrub sires, the removal of the corpus luteum will usually produce the desired results. This operation can probably best be performed as early as one can be certain the animal in question is pregnant. The following case report serves as an illustration of the re- lation of the corpus luteum and estrum: The patient, a purebred Aberdeen Angus cow, age four or five years, was reported to have failed to show symptoms of heat since her last calving. She calved successfully four or five months prior to the date on which we were consulted. An ex- amination revealed the presence of a corpus luteum. All other reproductive organs were believed to be normal. We removed the corpus luteum and advised breeding as soon as estrum ap- peared. Estrum was noticed and cow was served within a few days following the operation. She again appeared in heat at the termination of the dioestral period and was rebred. No further signs of heat developed and it was thought she was in calf. Near the termination of gestation she was examined for pregnancy, but was found to be open or not in ealf. There was a corpus luteum present and mild cervicitis was at this time de- tected. Since that time this animal has been under our close supervision, and up to the present time, in so far as we have been able to determine, has never appeared in estrum, except when we have removed a corpus luteum, which has been per- formed a number of times. We have allowed her to pass over three, four or five periods of heat, thinking possibly she might have conceived, but in each instance the examination for preg- naney showed that conception had not resulted. It is not of ——— Corpus LurrEuM AND EstTRUM 39 course entirely improbable that she may have aborted an embryo or two, but a close watch has failed to reveal any evidence of an abortion. The cervicitis responded to treatment, which has apparently had little or no effect on the act of estrum. During our studies of sterility we have not met with a closely similar case. Our experience shows quite conclusively that cows affected with sterility due to the presence of a per- sistent corpus luteum respond rapidly and regularly to treat- ment. Jn many cases conception takes place on the first service. There are, however, exceptions to this rule. RELATION OF Corpus LUTEUM TO PYOMETRITIS Pyometra is a condition of the uterus in which there are chronic inflammatory changes of the uterine mucose character- ized by sacculation of one or both of the horns and flaccid mus- cular walls with little or no contractile power due to a loss of muscle tone. The uterus is asymmetrical, abdominal in position, and one or both horns are partially filled or distended with pus. The cervix is inflamed and more or less dilated. This pathologic condition most often occurs in cows which have suffered with retention of the fetal membranes, but is known to occur in cases where the membranes were promptly discharged after calving. One of my colleagues working with the bacterial flora of this condition has in the large majority of cases been able to isolate Bacillus pyogenes, which is apparently the predomi- nating organism in these types of infection. The history of pyometra is as follows: The owner or herds- man in describing these cases states that the affected animal has not been in estrum since calving and that a small pool of pus is as a rule found back of her after she has assumed a recum- bent position. They also notice that there is more or less strain- ing of the lips of the vulva together with collection of pus on the under surface of the tail. In innumerable instances cows suf- fering with pyometra are allowed to go for a period of six or seven months before the services of a veterinarian are employed. In the treatment of pyometra, unless begun early, the prognosis should always be guarded, the reason for this being that even though the uterus undergoes complete involution and estrum is regularly established, the regeneration of the mucous membrane is so slow that attachment of the fertilized ovum is made diffi- 4) W. L. Boyp cult. Or in cases where the ovum does become imbedded, early abortions are not infrequent. The vetermarian who is experienced in the treatment of sterility fully appreciates the value of the dislodgement of the corpus luteum in the successful treatment of pyometra. In cows where the disease has been present only a short time the corpus luteum will frequently be found near the surface of the ovary, but in the long-standing cases the yellow body will be found to be more centrally located and therefore more difficult of ablation. The dislodgment of the corpus luteum in its en- tirety is responsible for such rapid changes in the uterus that one wonders at so much power being invested in so small a structure. Not only does the uterus promptly discharge its contents through the reestablishment of muscle tone, but it also changes rapidly, morphologically, regaining its symmetry and normal anatomical position within a very short time. Estrum frequently, though not regularly, appears within three to five days, especially in cases where tissue destruction has not been extensive. In the treatment of pyometra it is deemed best to siphon off the contents of the uterus before attempting to remove the yellow body. In this way the ovaries can be more readily and safely examined. The uterus can be retracted so that the ovary or ovaries can be without difficulty brought into the pelvic cavity, when the corpus luteum can be manipulated through the vagina. If the yellow body is deeply situated it should be re- moved per vagina, and no doubt this procedure should be fol- lowed regularly. We have, however, removed a large number of corpora lutea by pressure exerted through the rectal walls without bad results. In carrying out some experiments with pyometra we decided to remove the corpus luteum without at- tempting to siphon off the contents of the uterus, which, if performed, undoubtedly facilitates involution. The following cases are reported: No. 1—The patient, a purebred Jersey heifer, calved normally on February 10, 1921. The ealf was normal and the fetal mem- branes were promptly discharged. The vaginal discharge grad- ually lessened, lactation was thought to be normal, and the appetite was unimpaired. A few weeks after calving the herds- man noticed that usually in the mornings there would be a small pool of thick. cream-like pus in the gutter behind the heifer. ti eae Corpus Lureum anp EstruM 41 It was noticed also that there were accumulations of pus on the edges of the vulva and on the under surface of the tail. Estrum was absent. On April 22, 1921, we examined this animal and found her to be affected with pyometra. The right horn of the uterus was elongated, enlarged, and filled with pus. The left horn was apparently only slightly affected. A corpus luteum, which was located near the surface, was found in the right ovary. The external cervix was only partially dilated and did not permit of the escape of pus when the uterus was massaged. The re- moval of the corpus luteum was somewhat difficult, which was due to the fact that the ovum was earried so far forward into the abdominal cavity. The distended horn interfered with the operation only slightly. The yellow body was removed and torsion applied to the ovary until it was thought that the hem- orrhage had been controlled. The heifer continued eating and evidenced no signs of discomfort. The herdsman was advised that there would be a considerably larger amount of pus in the gutter by morning. She was operated on about 8:30 p.m. The next morning two or three liters of a grayish-white cream-like pus was found. The discharge continued for two or three days and ceased. Estrum took place four days after the operation. On April 30 she was again examined and found normal, the uterus having undergone complete involution. We advised breeding on the third or fourth estral period. Prognosis in this ease is favorable. No. 2.—The animal, a purebred Holstein-Friesian cow, five years of age, calved last on November 28, 1920. A normal healthy calf, but fetal membranes were retained. On March 26, 1921, she was examined and found to be suffering with pyometra. Cervicitis was found to be fairly extensive. Both horns of the uterus were enlarged, elongated, flaccid, and filled with pus. A large prominent corpus luteum was found in the right ovary. On March 28 we removed the corpus luteum by way of the rectum, and on the next day large quantities of pus were flowing freely. This material gave off an offensive odor. Cultures were made from the pus, which was obtained from swabs that had been introduced into the internal cervix and body of the uterus. Bacillus pyogenes was isolated without difficulty. April 2 estrum appeared, and in the uterus, with the exception of incomplete return of muscle tone to the right 49 W. L. Boyp horn, involution was almost complete. The cervix was treated regularly with Lugol’s solution undiluted. This cow rapidly grew better and was dismissed from the clinie April 15, 1921. Since going home she has been bred twice, and it is questionable if she is now in ealf. No doubt extensive tissue changes were present in this case, and regeneration of the uterine glands and surface epitheleum will be slow. I would not be surprised to hear later that this cow has aborted a two or three months old fetus. I would not advise early breeding in this type of cases, and the prognosis should be guarded. The manner in which the uteri of the above reported cases with such rapidity regained their normal structure and position is quite remarkable. No. 3.—A purebred Holstein-Friesian cow, aged 6 years, weight 1,400 pounds, in good physical condition, having calved last in October, 1920. She gave birth to a healthy ealf, but retained the fetal membranes, which were manually removed. We examined this cow on April 16, 1921, and found her to be affected with pyometra. No signs of estrum had been noticed up to this time. The symptoms observed here were typical of pyometra, and upon physical examination pathologie conditions or changes similar to the ones described in the second case were found. In this case the corpus luteum was located in the left ovary. Cervicitis was extensive with hypertrophy of the ex- ternal os. We operated on April 17, and on the 18th the uterus was found to be practically empty, the left horn was greatly reduced in size, being almost as firm as the right horn, and when stimulated by massage contraction was very noticeable. The os uteri, which was dilated in the beginning, was contracting slowly. The appetite remained good except that she refused grain feed following the operation. The milk flow increased markedly and signs of estrum appeared within a few days. Cul- tures taken from the pus from the vagina and cervix showed growths of Straphylococcus albus, scattered colonies of hemoly- tic streptococci and Streptococcus viridans. These types of organisms predominated. Colonies of Bacillus pyogenes were present, but not in large numbers. On April 25 this cow was practically normal. Clear mucus was discharged from the vagina, and the cervix was reduced in size and not so highly colored. April 30 we again swabbed the cervix with Lugol’s iodin solution, and as the patient was apparently normal she ~t Corpus LureuM AND EstruUM 43 was dismissed from the clinic. Prognosis in this case is guarded. We are of the opinion that she will again get safely with calf, although she may suffer an abortion before so doing. No. 4.—A purebred Guernsey cow, 5 years, weight 1,050 to 1,100 pounds; good physical condition; last calving date June, 1920. She gave birth to full-time but dead calf. Fetal mem- branes were retained. This cow was presented in our clinic January 13, 1921. Here was a case of approximately six months’ duration. On physical examination we found the os uteri par- tially dilated, permitting the escape of only small particles of pus when massage was applied to the uterus. The uterine horns were both greatly enlarged, flaccid, abdominal in position, and filled with an offensive smelling, creamy pus. Small amounts of this material were discharged when the cow was recumbent. A corpus luteum was removed from the right ovary on January 17, 1921, without producing any noticeable physical pain. The appetite was impaired for a day or two and somewhat capricious for perhaps a week, but she rapidly rounded to, and the milk flow was increased. On January 24 the uterus had undergone almost complete involution. It was then pelvic in position, but the right horn was still larger and longer than the left one. The cervix was treated at regular intervals, and while recovery seemed fairly rapid in this case, prominent signs of estrum dic not appear until March 7, 1921. A few days later we again examined this cow and, thinking that she had fully recovered, we shipped her home. Since leaving our clinic she has been in heat regularly and has been bred two or three times, but has failed to conceive. We examined her a few days previous to this writing and found that a large abscess had formed in the right ovary. The oviduct on the same side is probably also involved. The infection has no doubt extended along the mucous membrane of the tube, entering into the wound produced by the enucleation of the yellow body, or gaining access to the ovary at the time of rupture of a ripe ovisac. We advised ovariectomy, as the cow is a valuable one. Prognosis is unfavorable. THe Corpus LUTEUM AND MUMMIFICATION OF THE FETUS Mummification or desiccation of the fetus is not at all un- common in bovine practice, but is not of course as common as pyometra. Just what relationship bacteria bear to this phenom- enon has probably not been definitely determined. Bang reports 44 W. L. Boyp having isolated Bacterium abortus from mummified fetuses. It would seem to the writer that possibly Bact. abortus Bang is the causative factor in a certain percentage of these cases. A careful bacteriological study of desiccated fetus would be of great interest and value. W. L. Williams, in his recent publication, ‘*Diseases of the Genital Organs of Animals,’’ states that inter- placental hemorrhage with fetal desiccation is an interesting and an important clinical manifestation of placental disease. It has not been shown to be dependent upon disease of the nonpla- cental uterine areas. As a result of the hemorrhage the placen- tal membranes are forced apart, and the fetus, which succumbs, becomes surrounded by a hematoma which soon undergoes secondary changes until it becomes a soft, plastic, chocolate colored material surrounding the fetal envelopes and fetus, which in turn becomes mummified. The diagnosis of mummification of the fetus is not difficult. The history of the case is invaluable and the operator should give this due consideration in connection with the physical ex- amination. The uterine seal is well formed and the corpus luteum may be deeply imbedded, or, in cases of not too long standing, it will project to the exterior. The removal of the corpus luteum will in the large majority of cases cause an expul- sion of the uterine contents within three to ten days. In other eases where the yellow body has suffered with degenerative changes and the contractile powers of the uterus is at low ebb, the seal should be broken down and if necessary douching should be performed. In certain of these cases we have been able to re- move successfully the corpus luteum through the vagina, while in others it has been necessary to operate per rectum. We have never in these cases had to resort to ovariectomy in order to dislodge the corpus luteum, but no doubt this operation may have to be performed, especially in cases where the corpus luteum is small, centrally located, and difficult of manipulation. In the past two years we have operated on six of these cases, one of which, a purebred Guernsey cow, has been returned a. good breeder, having had one healthy calf since the operation. In another which we treated a long time for pyometra, concep- tion occurred early after treatment, but the fetus became mum- mified, and on account of her having been unable to produce a healthy calf over so long a period of time she was sent to Corpus LuTEUM AND EstTRUM 45 slaughter. The other cases, with the exception of those which were sent to slaughter, have not been followed up, so that I do not know of their ultimate outcome. The expulsion of the corpus luteum works wonders in these cases, and one should not depend upon other means of delivery. The corpus luteum bears a close relationship to both estrum and involution of the pathologic uterus, and the veterinarian who is doing cattle practice, if not already familiar with the workings of this gland of internal secretion, should immediately familiar- ize himself with it, for not only will he inerease his activities as a cattle practitioner, but his clientele will be more appreciative of his ability, and his services in other fields of practice will be more eagerly sought for. BIBLIOGRAPHY Born, G. Contributions to the Physiology of Mammalian Reproduc- tion : EscHer, H. H. Uber den Farbstoff des Corpus luteum. Zeitsch. f. Physiol. Chem., vol. 83 (1913), s. 198 FRAENKEL, L Die Function des corpus luteum. Archiv. f. Gynah., vol. 68 (1908), p. 488. PALMER, L. S., and KENNEDY, C. The relation of plant cartinoids to growth and reproduction of albino rats. Jour. Bio. Chem., vol. 46 (1921), p. 562. MARSHALL, F. H. A. The Physiology of Reproduction, 1910. MARSHALL, F. H. A., and Jotty, W. A. Contributions to the Physi- ology of Mammalian Reproduction. Philosophical Trans. Royal Soc., London, Series B, vol. 198 (1906), p. 99. Novak, £. The corpus luteum, its life cycle and its role in menstrual disorders. Jour. Am. Med. Assoc., vol. 67 (1916), p. 1285. WILLIAMS, W. L. Diseases of the Genital Organs of Animals, 1921. The Farmer says: There is no reason why livestock producers should not cooperate with the packers or any other marketing agencies in a campaign to increase the use of meat products. The fruit men of the West and the dairymen have pointed out the way to boost consumption, and they are profiting by the increased demand. Why not apply the same idea to the live- stock business? ‘ National Stockman and Farmer says: Where are the trot- ters necessary to entertain the crowds at county fairs to come from five or ten years hence? Not enough are being bred to keep up the horse population. Better breed that well-bred mare. SOME OBSERVATIONS RELATING TO SEX DETERMI- NATION AND THE EMBRYOLOGY OF THE UTERUS ' By J. P. Foster Minneapolis, Minn. NOT MANY YEARS have elapsed since biologists quite gen- erally entertained the opinion that sex determination did not occur at the time of the fertilization of the ovum. It was held that Nature did not decide this important matter until the embryonic stage was fairly well advanced. This belief was strengthened by the fact that the Wolffian ducts and the Mul- lerian ducts—forerunners of essential portions of both the male and the female generative organs—are present in all normal embryos, and it was supposed that, after a decision had been arrived at, one set of sex organs progressed to full completion, while the embryonic structures representing the generative organs of the opposite sex underwent no further development and became atrophice. In the male the Wolffian ducts go to form the excretory chan- nels of the testicles, and vestiges of the Mullerian ducts are represented by the uterus masculinus. In the female the Mulle- rian ducts develop into the Fallopian tubes, the uterus and the vagina, and vestiges of the Wolffian ducts are represented by the parovarium and the canals of Gaertner, found’ in either side of the walls of the vagina. The fact that hermaphroditism occasionally occurs apparently corroborates the hypothesis of delayed sex determination and might lead to the supposition that the condition is referable to indecision on the part of Nature, resulting in the partial or im- perfect development of both male and female generative organs. Although it must be admitted that embryos do possess elemen- tary structures from which organs of either sex may develop, this is not proof that sex was not determined at the time of the fertilization of the ovum. Neither does hermaphroditism prove delayed sex determination, for the reason that certain influences might be brought to bear upon the growing fetus which could 1Presented at the twenty-third semi-annual meeting of the Minnesota State Veterinary Medical Association, Hutchinson, Minn., July 14 and 15, 1921. 46 Sex DETERMINATION AND EMBRYOLOGY OF UTERUS 47 interfere with or modify the normal development of sexual or- gans in spite of definite sex determination at the time of con- ception. In the light of more recent investigations it would seem that sex determination takes place simultaneously with the fertiliza- tion of the ovum. The scope of this paper will not permit of reviewing the phe- nomenon of mitosis in ordinary tissue cells, the important de- tails of which are more or less familiar to all. Suffice it to say that the present-day theory of sex determination is based in part upon this phenomenon. A certain definite number of chromosomes have been demon- strated in the nuclei of the body cells of different species of ani- mals. For instance, man has 48, the hog has 40, Ascaris meg- alocephala has 4, and one of the grasshoppers has 14. These numbers apply to the chromosomes of tissue cells, but not to those of the ova and the spermatozoa, the so-called functional reproductive cells. The reproductive cells undergo a process of maturation so that finally they contain only half the definite number of chromosomes required by the tissue cells of the species. The reason for this reduction is that while a tissue cell contain- ing, say, 40 chromosomes divides so that each chromosome is split into two, each resulting in a daughter cell containing the original number of 40, it should be remembered that the chromo- somes derived from the nuclei of both the ovum and the sperm cell are present and take part in the phenomenon of fertilization. Therefore only half the number of chromosomes is needed from each source to make up the total required by the body cells of the species. It will be noted that cell division in growing tissue cells is a much simpler process than the phenomenon of the fertiliza- tion of an ovum by a sperm cell. The latter implies either fusion of the individual chromosomes or their arrangement in such a manner that when cell division takes place half of the echromo- somes furnished by the ovum and half of those derived from the sperm cell will be present in each of the two daughter cells, marking the beginning of a new being embodying the heredi- tary properties of both parents. Investigators have shown that in a certain variety of grass- hoppers the cells taken from different tissues of males contain 6 pairs of chromosomes of similar appearance and one chromo 48 J. P. Foster some differing from the others, making a total of 13. The tissue cells of females show 7 pairs of chromosomes, 6 pairs of which are apparently like the 6 pairs found in the male, and one pair resembling the odd or thirteenth chromosome of the male, mak- ing a total of 14. The female reproductive cells before their division into the functional reproductive cells (ova) contain the same number of chromosomes as the. tissue cells just mentioned. However, after the final division the mature ovum contains only 7 chromosomes, 6 of which are alike, and one odd one, which represents, of course, one-half of the 6 pairs of similar chromosomes and the one pair of odd chromosomes found in the reproductive cell before its division to form.the functional reproductive cell. As already stated, the tissue cells of the male contain 13 chromosomes, which number applies to the re- productive cells before the final division into the functional reproductive cells, the spermatozoa. It is evident that there must be two kinds of sperm cells occurring in equal numbers: one kind containing 7 chromosomes, the other containing 6, as a result of the division of a reproductive cell containing 6 pairs of chromosomes and one odd chromosome, or 13 in all. If an ovum which invariably contains seven chromosomes is fertilized by a spermatozoon containing 6 chromosomes, an individual will develop containing 13 chromosomes in its body eells, there- fore a male. On the other hand, if an ovum is fertilized by a spermatozoon containing 7 chromosomes, an individual contain ing 14 chronosomes in its body cells, or a female, is the result. The odd chromosome in the ovum and the odd chromosome in the female determining spermatozoon are referred to by biolo- gists as the accessory, or X, chromosome. In the human, ac- cording to Van Winewarter, the male-determining sperm cell contains 23 chromosomes, while the one determining female sex contains 24. The matter of ‘‘identical twins’’ is interesting on account of the probable relationship to sex determination. In ordinar twins two ova are fertilized. These ova result from the rupture of two Graafian follicles in the same ovary, or from two Graafian follicles one of which is situated in each ovary.’ Three possi- bilities are presented. First, each of the two ova may be fer- tilized by a male-determining sperm cell, resulting in twin males. Second, each of the two ova may be fertilized by a female-determining sperm cell, resulting in twin females. Sex DETERMINATION AND EMBRYOLOGY OF UTERUS 49 Third, one of the ova may be fertilized by a male-determining sperm cell, and the other ovum may be fertilized by a female- determining sperm cell, resulting in twins, one a male, the other a female. On the other hand, ‘‘identical twins’’ are the result of a complete division of one ovum or blastoderm, resulting m the formation of two distinct embryos. As sex was deter- mined when the ovum was fertilized, prior to its division, the twins are of the same sex. Each has its own amnion, but they are inclosed in one chorion. The common chorion is considered proof of this form of pregnancy. A prominent writer on the subject of genetics recently made the following observations: Breeders of dairy cattle in particular often become discouraged with a bull which seems to sire largely bull calves. It must be re- membered in this connection, however, that rather large departures from equality may occur simply by chance. Thus if a coin is tossed 20 times, the best expectation is 10 heads and 10 tails, but about once in 40 times a departure as great, or greater, than 15 heads or tails is to be looked for. Thus a large number of dairy cattle breeders may be expected to get 15 or more bull calves out of 20 calves born. Such a result in one year would not have the slightest effect on the sex ratio the next. Another well-known authority states that ‘‘sex seems to be determined by one of the chromosomes, and is probably settled at the time of conception. The determination of sex is there- fore a matter of chance over which the breeder has no control. For practical purposes, the breeder will find that, over a period of years, he gets nearly equal numbers of both sexes.”’ Reference has been made to elementary structures known as the Mullerian ducts, found in all normal embryos. In animals having rather prolonged periods of gestation, such as the mare and the cow, they appear early in the second month of pree- nancy as two rods of mesoblastie tissue arising in the right and left sublumbar regions, converging caudally in the form of a V near the posterior common body opening or cloaca. The rods be- come hollowed out to form tubes, and near the end of the second month the extremities of the ducts, which already are in contact with each other, become fused. The coalescing of the two tubes —right and left—results caudally in the formation of a single medial tube, the length of which depends upon the extent of the fusion; and the structures which at first resembled a V in form now resemble the capital letter Y. The opening of each duct into the peritoneal cavity in the sublumbar region repre- 50 J. P. Foster sents the future ostium abdominale of the Fallopian tube. The fused medial tube represents the future vagina and, in those species in which the fusion progresses beyond this point, the body of the uterus. The following observations relative to the uteri of various species are taken from Williams’s ‘‘ Veterinary Obstetrics’? : Derived from the ducts of Muller, it (the uterus) varies greatly in form and disposition, partly dependent upon the degree of fusion between these two structures. There are generally recognized a uterine body and two cornua, each having essentially like functions in varying degrees. In the rabbit there exists no uterine body, but two distinct tubular uteri, opening separately into the vagina. In the bitch, cat and sow the uterine body is limited in extent and physiologically unimportant, rarely containing even a portion of a fetus except in transit at the time of birth, while the two cornua are extensive, and in them de-. velop virtually all fetuses so that, physiologically, they represent the two separate uteri of the rabbit. In ruminants the uterine body becomes markedly greater in size, and assumes far higher importance physiologically, while the cornua remain relatively large. In these animals the one, two, or more fetuses habitually rest almost equally in the body and cornua, so that they represent a middle point in the transition between the double uterus of the rabbit and the single uterus of woman, physio- logically devoid of cornua. In the mare the uterine body becomes relatively more important than in the ruminant. The relative importance of the cornua to the uterine body sustains a close relation to the number of young brought forth at a given birth. In multiparous animals there are two uteri, as in the rabbit, or the two extensive cornua with functionally unimportant uterine body, as in the bitch, cat and sow. In ruminants, which are largely biparous, the uterine body and cornua become approximately equal in extent and function. ; In the normally uniparous mare the cornua are smaller than the body, while in woman the uterus is virtually without cornua in size or function. \ In the larger animals, like the cow, size of the parts readily permits of extensive rectal palpation and manipulation of the uterus, tubes and ovaries, thereby making it possible to carry out the excellent work that is being done at the present time in the early diagnosis of pregnancy and the treatment of sterility. Washington Post publishes a cablegram from Paris to the ef- fect that ‘‘Bully beef, from Chicago, known to the poilus as ‘tinned monkey,’ may be the poor man’s menu this winter. The Department of Agriculture is suggesting a series of propa- ganda campaigns to develop an appreciation for tinned meats.”’ SOME SURGICAL OPERATIONS ON THE OX' By H. HE. Bemis Professor of Surgery, lowa State College, Ames, Lowa IN presenting this paper it is my intention to outline treat- ment for some of the common surgical conditions affecting the ox, excluding from the discussion the surgery of the repro- ductive organs, which has been so well presented before your association at recent meetings. The conditions presented in this paper are those which need special consideration on ac- count of the differences in the anatomy of the region affected from the anatomy of the same regions in the horse, with which we are probably more familiar. We all realize that the present trend of affairs is constantly leading the veterinarian toward greater practice on conditions affecting the food-producing animals, and it is extremely important that the special prob- lems affecting each species be emphasized. DRAINAGE OF THE FRONTAL SINUS The frontal sinus of the ox is very different from that of the horse, particularly with respect to its extent superiorly and the fact that it is partially divided by many bony septa, which makes direct drainage through the cavity impossible. Aside from the usual description of the frontal sinus as given in Sisson’s Anatomy, I wish to call attention to the follow- ing facts: 1. The frontal sinus in many specimens extends posterior to the region of the poll or frontal eminence and laterally to a point just anterior to the external ear. 2. The deepest and freest portion of the frontal sinus is found about midway between the base of the horn core and the median line. The cranial plate arches upward in the cen- ter of the skull inferior to this free portion so that the cavity is limited to the lateral borders on a line drawn across the nar- rowest portion of the head, about midway between the pos- terior border of the orbital cavity and the base of the horn. 1Presented at the twenty-third semi-annual meeting of the Minnesota State Veterinary Medical Association, Hutchinson, Minn., July 14 and 15, 1921. 5] 52 H. E. Bemis In the plane which passes through the posterior borders of the orbital cavity the sinus again swings toward the median plane and at this point constitutes the second largest cavity and marks the lower lmit of the frontal portion of the sinus. Upon a plane passed through the middle of the orbital cavi- ties, the frontal portion of the sinus is usually separated from the turbinal portion of the sinus by a complete septum. The same idea may be expressed in a different and per- haps clearer way by stating that if a horseshoe having wide webbed branches and a narrow toe should be placed upon the ox’s head so that the upper branch would rest upon the median line in the region of the poll and the toe of the shoe would rest upon the lateral portion of the frontal region at its narrowest point, the lower branch would rest over the second most free portion of the sinus and any point covered by the shoe would be a safe point for trephining the sinus. 3. On a plane passing through the narrowest portion of the region a small opening about the size of a lead pencil passes from the floor of the sinus into a compartment which is separated from the temporal fossa only by a thin plate of bone. In ease pus is retained in this compartment, the bone sometimes becomes necrotic and an abscess develops, posterior to the eye and occupying the temporal fossa. 4. From the lower limit of the frontal portion of the sinus on a plane passing through the center of the orbits and about one and one-half inches from the median line, the frontal sinus communicates with the nasal cavity through the ethmoid cells. From a trephine opening located as just described, drainage may be established through the nose by passing a curved instrument from above to below and directed slightly toward the median line. The frontal sinus of the ox usually becomes infected fol- lowing accident to the horn or dehorning operations. In these cases one usually has a means of entrance to the superior free portion of the sinus. The other two points where it is most practicable to trephine the sinus for further drainage are over the toe and the lower branch of the horseshoe-shaped area already described. From the first of these the compartments in the temporal fossa may be flushed, and from the second one bottom drainage may be established through the nasal eavity. If a jee! |. Se DA Clee eA ae a = 4 ae ‘ SURGICAL OPERATIONS ON THE OX 53 an abscess forms in the temporal fossa it may be drained by an incision through the skin at the most prominent point. Such drainage is not complete and usually constitutes a tem- porary relief until animals can be marketed. EXCISION OF THE EYE The frequency of the occurrence of accidental wounds, ab- scesses and malignant tumors of the eye of the ox often makes it necessary or desirable to remove the entire structure. We believe that the most satisfactory method of performing this operation involves the complete removal of the conjunctiva so as to prevent the formation of secretions which result in constant discharge. To perform the operation, confine the animal on the table or ground, and carefully remove, by shaving, the hair of the lids and surrounding area and paint the surface with tincture of iodine. With a small hypodermic needle inject subcutane- ously a liberal amount of local anesthetic, such as 1 per cent solution of stovaine, apothesine or procaine, containing adre- nalin chloride in a 1-to-5,000 solution, into the upper and lower lids, about 1 em. from the border of the lids. Follow this by injecting about 1 ¢.c. of the same solution at four different points into the depths of the region posterior to the orbit. Suture the upper and lower lids together with a continuous ‘silk suture, confine the skin of the lids with tissue forceps, and with a very sharp knife make an incision through each lid about a quarter of an inch from their borders, uniting the incisions the same distance beyond the lateral and medial eanthi. The incision should pass to but not through the con- junctiva. The hemorrhage should at all times be carefully controlled so that this technique may be carried out after com- pleting the incisions to a uniform depth throughout their extent. The conjunctiva is separated from the lids back to its attachment to the orbit. This leaves the conjunctiva at- tached to the border of the lids; which have been sutured together, and these tissues may then be used to apply attrac- tion upon the orbit. After the dissection has been carried back to the point of insertion of the conjunctiva to the orbit, the muscles of the eye are clipped with a pair of curved scis- sors and finally the optic nerve is cut in the same manner and the orbit completely removed, the third eyelid being re- 54 H. E. Bemis moved with it. All of the periorbital fat should be left in place. The hemorrhage, which is not great, should be con- trolled with sterile gauze sponges and all blood clots removed from the cavity. The cavity is then packed with sterile gauze dusted with equal parts of chlorinated lime and boric acid well mixed together, or iodoform, and the cut edges of the lids are carefully sutured with interrupted silk sutures beginning at the external angle, omitting the last stitch to provide drain- age. Remove the pack in twenty-four hours and inject into the cavity about one-half ounce of Bipp, which is a paste composed of iodoform 16 ounces, bismuth subnitrate 8 ounces, liquid paraffin 8 ounces. Very little after-treatment will be found necessary. The cavity will fill and the edges of the lids become permanently healed together in about two weeks. The area is finally left somewhat sunken, but the surface is absolutely smooth and no discharging wound remains. ACTINOMYCOSIS IN THE MANIBULAR AND PAROTID REGIONS Actinomyecosis affecting the glands of this region is quite common in cattle and often calls for some form of surgical pro- cedure. There are two methods of dealing with this econdi- tion. The one involves complete removal of the diseased tissue and is indicated in case of circumscribed tumors in valuable animals which are to be kept for a considerable period of time. The second involves drainage and proper after-treat- ment and is indicated in case of butcher animals which are being fed for market. Here it is of advantage to use a method which will control the condition and at the same time incur the least possible loss of time and flesh. Without enumerating the many problems connected with complete removal of such tumors, due to the large number of important structures in this region, I wish to emphasize a few important points in connection with the technique of the oper- ation. 1. After thoroughly preparing the region and locating as far as possible structures to be avoided, a liberal elliptical incision should be made in a longitudinal direction, which should include any and all fistulous openings and should be large enough to remove all excess of skin due to the enlarge- ment. The incision should be carefully carried to the line of SURGICAL OPERATIONS ON THE OX 5. ~ cleavage between the normal and diseased tissue. This line can be discovered most easily by beginning the blunt dissec- tion as near the base of the tumor as possible at some point from which it will be more easy to work out in all directions than to attempt to find the line of cleavage over the periphery of the tumor. Following the line of cleavage with blunt dissection, using the scalpel handle or Mayo scissors so far as possible, it should be possible to recognize any important vessels, ducts or nerves before they are sectioned. Vessels which must be cut should be ligated before being cut. Large vessels should be ligated at two points and then divided between these two points. The separation should continue until the tumor has been com- pletely isolated. It is usually found that a small neck of diseased tissue extends into the depths of the region be- yond the large portion of the tumor. This should not be eut across but completely removed. ; If the wound area is large and important vessels are ex- posed, the tissue spaces should be closed and protected by suturing the tissues together in the depths of the wound. If there is a parenchymatous hemorrhage, a sterile gauze pack should be placed in the cavity and the skin united with in- terrupted sutures. It is important to warn the owner against the danger of post-operative hemorrhage, and to prevent this the animal should be closely confined in a stall where there is no opportunity for the animal to place pressure on the jugular region. A course of potassium iodide treatment may be given as a further safeguard against recurrence. 2. The second method has been found to be very practical and satisfactory in case of animals in the feed lot. It con- sists in thorough drainage of the process, followed by paint- ing the cavity with tincture of iodine and then packing the eavity with gauze upon which has been smeared about halt an ounce of paste made of equal parts of zine chloride and sanguinaria. This pack should be sutured in place and allowed to remain for five to eight days, after which it is removed, together with the slough which has been caused by the pack. If practicable, it is well to carry out the potassium iodide treatment in these cases as well. Under such treatment we have found that the tumors make either complete recovery or sufficient reduction takes place so that the animal can be 56 H. EK. Bemis marketed without loss, as there is very little if any loss of flesh due to the operation if an animal can be handled in the stocks or chute. For these reasons we believe this method to be practical, although not so satisfactory from a surgical stand- point. | RUMENOTOMY IT will not attempt to describe the indications for rumenotomy, but wish rather to direct attention to some of the important points in performing the operation. Many times the animal is in such a toxie condition that anesthesia is not necessary and the problem of control does not enter into consideration. If this is not the case, satisfactory anesthesia can be obtained by the use of local anesthetics injected subcutaneously along the line of incision. The animal may be controlled either in a standing or recumbent position, according to the circumstances. After shaving the region. of the left flank, the area should be carefully sponged out with bichloride of mercury, 1 to 1,000, and painted with tincture of iodine. The structures to be di- vided, named in order from without to within, are as follows: 1. Skin. 2. Subeutem and subeutaneous fat in variable amounts. 3. The external oblique muscle. The fibers of this muscle pass the region in very nearly a horizontal direction and are relatively thin. 4. Internal oblique muscle, and those arising from the ilium, whose fibers are considerably thicker and pass in a direction downward and forward, from the external angle of the ilium. A definite branch of the circumflex artery passes along the superior border of this portion of the muscle. 5. ‘Transverse muscle of the abdomen, which in this region is entirely aponeurotic. The fibers extend in a perpendicular direction and are white and glistening in appearance and very thin. 6. The iliac fascia. This structure is thin and yellowish in appearance and is separated from the previously mentioned structure by aureolar tissue. 7. The subperitoneal fat. 8. Peritoneum. The importance of knowing the various structures which are found in this region is that the operator may be able to recognize each structure by its color and direction of fibers and consequently may know at each step in the operation just how far the operation has progressed. _ ——_——a . ¢€ or =~! SuRGICAL OPERATIONS ON THE OX A perpendicular incision about 8 to 10 inches in length, starting at a point about equal distance from the last rib, the lumbar vertebre and the external angle of the ilium, should be made through the skin, subcutem and external oblique muscle. The fibers of the internal oblique should be sepa- rated in their long direction, across the center of the wound, and likewise the fibers of the aponeurosis of the transverse muscle and the iliac fascia should be divided in the same direction as a skin incision. After separating the fat the peritoneum should be picked up with forceps, incised with scissors or scalpel, and completely divided with a blunt bis- toury. The divided edges of the peritoneum should be secured with forceps, which are allowed to remain in place during the operation. A liberal strip of sterile gauze should be placed within the peritoneal cavity, through the lower commissure of the wound, and finally the rumen divided liberally and the divided edges fixed with forceps or tape. Under this method a free opening is made through the abdominal wall and into the rumen and much less tissue is actually destroyed than by trying to operate by separating all of the muscles in the direction of their fibers. After emptying at least two-thirds of the contents of the rumen the edges of the wound in the rumen should be care- fully cleansed and the wounds united by using one row of interrupted or continuous silk sutures to adapt the edges. This line of suture should then be buried for a distance of one-half to three-fourths of an inch by the use of a catgut Lembert suture, No. 2 or No. 3, passed in with a round- pointed needle, attempting to avoid passing the needle through the mucous membrane. Particular care should be taken to get equal apposition of the peritoneal surfaces, and especially so at the commissures. Within twelve hours following the oper- ation a line of sutures so placed will be completely covered by a wound exudate and adhesion will begin to form. After removing the gauze and preparing the wound edges, the peritoneum, still fixed in the forceps, should be pulled up and sutured with catgut. A few catgut sutures may be used to unite the muscle wounds or to obliterate spaces within the tissues, and finally the skin should be sutured with silk, leaving drainage in the lower angle of the wound and the 58 H. E. Bemis line of suture protected with gauze and collodion except at the drainage opening. The points which I wish to emphasize particularly are the importance of the careful apposition of peritoneal surfaces, both visceral and parietal. I am thoroughly convinced that no abdominal wound is properly cared for which does not include these two precautions. The complications may be peritonitis, abscesses in the wall of the abdomen, and fistula of the rumen. Peritonitis should be treated on general principles and abscesses treated by careful and complete drainage. By using catgut sutures in the depth of the wound I believe the number of abscesses can be reduced. If fistula results, the process should be allowed to heal as completely as it will, and if complete healing does not take place, the final tract should be curetted and cauter- ized with a hot iron or pure phenol, after which healing usu- ally takes place. AMERICAN TO DIRECT NEW BRAZILIAN COLLEGE Dr. Peter Henry Rolfe, of Florida, has been appointed to direct the agricultural and veterinary college which is to be established in the State of Minas Geraes, Brazil. Ambassador Edwin N. Morgan reports that a credit of 1,000,000 milreis has been authorized to meet the initial expenses of the school. U.S. COWS SUPERIOR The American milk cows which have been distributed through Bavaria are declared to be better milkers than the German breed. The Bavarian farmers express astonishment that the American cows give about twenty quarts of milk daily after their first calf, which is double the quantity of German cows. NEW BUILDINGS AT AUBURN, ALA. The contract for the construction of three new buildings at the Alabama Polytechnic Institute at Auburn, Ala., has been awarded. The transaction involves $125,000 and the structures are to be completed by January 1. One of the buildings will replace Comer Hall, the big agriculture building. The other structures will be used for veterinary science. PROGRESS OF BOVINE TUBERCULOSIS ERADICA- TION IN MONTANA By W. J. BUTLER State Veterinarian, Helena, Montana IN 1911 the Twelfth Legislative Assembly of Montana en- acted Chapter 146, “‘An act to provide for the tuberculin test- ing of bovine animals, their quarantine, destruction, and dispo- sition under the general supervision of the Livestock Sanitary Board.’’ This law specifically required that all dairy cattle be tuberculin tested and that all reactors be destroyed or segre- gated and quarantined. The difficulties that presented themselves in fulfilling this work were many. Montana is the third largest State in the Union; its ranches and dairy herds are widely separated. Prac- tically none of our dairy herds are kept under pasture the entire year around. When cattle are dry they are turned out on the adjacent range. In the foot-hill country barns are made of logs, and in many sections where milking is done only during summer months there are no barns at all. In addition to this, due to the many settlers coming into the State, there are a number of one-cow dairies which carry on a dairy business from time to time as cows come fresh. Taking all of these adverse conditions into consideration, it will at once become apparent that the progress made by the State of Montana in cleaning up its dairy herds and eradicating tuberculosis in livestock is indeed re- markable. It gives a concrete example of what may be accom- plished by an active and consistent campaign in the eradication of an infectious contagious disease. The following table is the official record of the number of animals tested, the number of tuberculous animals found, and the percentage of tuberculosis for the years 1911 up to and in- cluding June 30, 1921: a9 60 W. J. BUTLER Tuberculosis Year Tested Reactors Percentage VOD Ds 7a rete 8 2) 799 829 10.68 uP ae Oe etl 4 a re 7,158 481 6.7 Gut pervert Ae Be tee, 6,769 371 5.4 GWA ere a eR Ren Me ee 5,788 174 3.0 I 3 os. sey TC A 5) dake 345 2.4 TOG 125 lak te 5 3 ne . 9,988 520 5.2 THY eee eet ee 13,781 707 5.2 DOTS Ac Sh oe 22,720 1,151 5.0 1 LS ee A Oe 26,661 752. 2.82 NO 2 Opes eee 29,017 647 2.22 1921 (to June 30)............... 29,990 260 0.86 Migraltre fect. hk 173,517 6,237 3.5 It will be noticed that when tuberculin testing was first inau- gurated 10.63 per cent of reactors (or tuberculous animals) were found. The second year was practically a retesting of the herds that had been tested in 1911. The percentage fell to 6.7. The testing of 1913-1914 was a repetition of the previous years’ test- ing, in that methods of testing and funds did not permit an ex- pansion of the work or the testing of new herds. Tuberculosis in the herds that had been tested fell to 3 per cent. On September 21, 1914, the intradermiec tuberculin test was officially recognized. This test is commonly ealled the ‘‘tail test.’? Previous to this time the States of Missouri and Cali- fornia had used the intradermic test, but Montana was the first State to adopt it officially as its recognized test and to guarantee its accuracy by paying the owner of any animal falsely con- demned (through the use of the intradermic test) the full and true book value of the animal condemned. I think I may safely state that bovine tuberculosis in western States or in ‘semi-range States will never be eradicated by the use of the old subeuta- neous or temperature test. This for the reason that where the temperature test is used only gentle and well broken animals can be accurately tested. With the intradermic test any animal, wild or gentle, young or old, may be accurately tested. With the adoption of the intradermie test the Livestock Sani- tary Board issued an order that all cattle in any herd, young and old, wild and gentle, must be tested. The intradermic test brought to light the fact that calves may suffer from tubereu- losis. It also demonstrated the fact that many dry dairy animals that were running out in the hills were also tuberculous. These calves and dry animals could not be tested with the subcutaneous test, and therefore until the adoption of the intradermie test ye < TUBERCULOSIS ERADICATION IN MONTANA 61 they were a constant source of reinfection to that particular herd. Strange as it may seem, we have found generalized tuber- culosis in calves six weeks old. Such an occurrence hardly seems possible, but it is an absolute fact. With the use of the intradermic test we were able to test more than twice as many cattle for the same amount of money as we had been able to test in previous years. In 1915 the percentage of reactors fell, even though we tested a number of new herds. The work in 1915 was expanded by testing in the Flathead district, where we tested thousands of cattle without finding one reactor. This is the principal reason for the percentage falling. Even though our percentage of reactors fell in 1915, we found considerable tuberculosis in previously tested herds. We realized there must be some outside source of infection. In most in- stances we found that their purebred bulls, which had been imported into the State since our last previous test, were tuber- eulous. These bulls had been shipped into Montana accom- panied by an official tuberculin test chart issued by the State from which they originated, stating that the animals were free from tuberculosis and any infectious contagious disease. To protect the stock interests of Montana the Livestock San- itary Board issued an order that all purebred cattle shipped into the State would have to be shipped into quarantine and held subject to a 60-90 day retest. Our figures for 1916 were a revelation. Out of 1,646 purebred animals shipped into the State, accompanied by an official tuber- eulin test chart, we found when we subjected them to our 60-90 day retest that 145 were tuberculous. No wonder that our herds were becoming reinfected when 8.8 per cent of all purebreds being shipped into the State, even though accompanied by a elean bill of health and tuberculin test chart, were tuberculous. In 1916 the States of Minnesota, North Dakota and Montana held a meeting to protect themselves against the fraudulent im- portation of purebred cattle from ontside States. This meeting . provided for the 60-90 day retest in these respective States, and 1 feel safe in stating that the nation-wide tuberculosis eradica- tion campaign is the result of the action taken by the States of Minnesota, North Dakota and Montana. It will be noted that in 1916 our percentage of reactors jumped to 5.2 per cent. The principal reason for this was the testing - of imported purebred cattle, and also to the testing of herds 62 W. J. BUTLER where we knew they had recently acquired imported sires. It will be noticed that our percentage of reactors held fairly even for the years 1916, 1917 and 1918. Due to the enormous dis- tances to be traveled, to climatic conditions, and the financial condition of the Livestock Sanitary Board it took us practically three years to clean up our herds which had been contaminated from outside sources. It might have taken us longer had not the United States Bureau of Animal Industry in 1917 estab- lished its cooperative tuberculosis eradication campaign. We did not receive very much help in 1917, but we did receive con- siderable help in 1918, and ever since that time have received efficient and well directed cooperation from the United States Bureau of Animal Industry. With the knowledge of the work accomplished and with the careful studying of our official records one is safe in stating that the peak load of our work in bovine tuberculosis eradication has been reached and that gradually, but surely, bovine tuberculosis in Montana is being eradicated. The figures for the first six months in 1921 indicate beyond words the progress of our work. We have tested 29,990 cattle and have found but 260 tuberculous animals, a percentage of 0.86. In 1922 even this percentage should be decreased, and we sincerely trust that each year it will grow less and that the work of the Livestock Sanitary Board will be crowned with the achievement of having eradicated bovine tuberculosis in the State of Montana. F During the past ten years we have destroyed 6,237 cattle which could have been and which were in many cases a source of infection to the human family. It must also be realized that had these animals been permitted to live they would have infected countless other bovine animals, which in turn would have been a source of infection to the human family. Taking these facts and figures into consideration, the milk-drinking public must realize the protection which is being given them and their children by the Montana Livestock Sanitary Board in the eradication of tuberculosis. The fact that in 1921 we found only 0.86 per cent of tuber- culosis in all cattle tested is a positive assurance to the State and the world in general of the cleanliness and purity of Montana dairy products and the healthfulness of Montana livestock, PARTURIENT PARESIS OF THE DAIRY COW: By A. A. Morey Alpena, Mich. PARTURIENT PARESIS is a disease affecting especially the dairy cow following parturition, rarely before or during birth, and is generally of a nonfebrile nature, resulting in a general paralysis, or, in nontreated cases, death. The cause of this disease is still unknown. Although many theories have been advanced as to the cause, none of them have been generally accepted. The first symptoms are generally manifest from 12 to 72 hours following the birth, although cases are occasionally met where the first symptoms are noticed within an hour following the birth, and still more rare where several weeks have elapsed. It is a noticeable fact that cases that develop extremely late more often develop tympanites and coma early during the attack. These late cases may or may not be true parturient paresis; in fact, they rather suggest auto-intoxication, but the fact remains that they do answer favorably to the same treatment employed in the typical cases. The earliest symptoms probably noticed are greatly decreased lactation, uneasiness, treading with the hind feet, possibly getting up and down. This uneasiness continues from one-half hour to several hours, when animal reaches a state when she can no longer stand and goes down, lying on her sternum with her head to her side, tears wetting the face and presenting a picture of absolute helplessness, and if left alone, will gradually pass into a state of coma, when the position is changed to lying flat on the side. The temperature, while generally subnormal, is oc- easionally found to be as high as 103° F. I will now discuss the different treatments I have used and results given. During my early experiences the Schmidt treatment was used, with varied results. The percentage of recoveries with this treatment was not nearly so great as with the air treatment, and ‘Presented at the fifty-eighth annual meeting of the American Veterinary Medical Association, Denver, Colo., September 5-9, 1921. 64 A. A. Morury sequele were far more numerous. Many would gain their feet only to die of pneumonia in a few days; others would develop mammitis, eventually the gland function being lost, and those that did recover did so very slowly. These sequelae were met regardless of how strict aseptic precautions were employed when treatment was administered. The next treatment was the trikresol treatment advocated at the time by Dr. Samuel Brenton of Detroit, Michigan. This treatment consisted of trikresol and glycerin, equal parts, 2 drams of this mixture in a quart of sterile lukewarm water and distributed equally in the four quarters of the udder. This treatment gave excellent results, with no sequela; in fact, it proved the next best to the sterile air treatment. The past few years we have had the sterile air treatment and the oxygen treatment. My experience has been entirely with the sterile air, which, on account of the convenience and grati- fying results, has been all that could be desired. The results from this treatment have been so favorable that many think that all that is necessary is to inflate the udder of the patient and she will recover. Granting that this is true in the majority of cases, it is for the few that will not, that we deem the following important : The position in which the patient is kept during the attack is practically as important as the air treatment, as the cow whose udder is inflated and then allowed to remain lying on her side, as they do in the comatose state, has little chance for recovery. The first thing upon arrival is to roll animal upon her sternum and insist that she be kept in this position during the entire time she is unable to stand. One-half a grain of strychnin sulphate is then given subcutaneously, and repeated in six hours if neces- sary, but I never give more than half a grain for initial dose and sometimes less if the animal is of the small type. The bovine does not tolerate strychnin as the equine, many seeming pecu- ilarly susceptible to its action. Some practitioners prefer atro- pin, but personally I prefer strychnin, except in those cases where the patient seems very excitable, or those that occur on hot, sultry days. Cases on such days always deserve a more guarded prognosis. The udder is then inflated after washing the teats and tube with a mild cresol solution. The teats are not tied, as by press- PARTURIENT PARESIS OF THE Dairy Cow 65 » ing the end of the teat for a few seconds the air will cease to escape. If a case is met where it seems necessary to tie the teats, a piece of gauze bandage may be used, but should only be allowed to remain for a short time. Milking before inflating is not necessary, as a very small amount of milk is present. In connection with the inflation of the udder, I wish to con- demn the use of the bicycle pump as an instrument for this pur- pose. This practice should be discouraged, as it leaves a bad impression with the client and it may injure the mammary elands. Hemorrhage within the glands has been known to occur following the vicious use of a pump. It is practically impossible to overinflate the udder with a small hand bulb outfit. If patient does not recover in six hours the udder is reinflated. The cylinder of a bulb outfit should be filled with absorbent cotton which is dampened with a few drops of trikresol or other suitable antiseptic. Personally I am in favor of trikresol for this purpose because of the results ob- tained with the trikresol treatment. I have had a few cases die while in the act of inflating the udder, and for this reason it seems best to administer the strychnin before the air, as since following this procedure such a misfortune has never occurred. After completing all work with the udder, if placental mem- branes are still retained they should be removed, which, in my experience, is always easily done. The membranes are usually detached from the cotyledons in these cases and lying in the uterus, and when they are attached they can easily be removed by shght traction. | It is important that the attendant be instructed not to milk until the patient has been on her feet and apparently normal in every way for at least three or four hours, and not to give or allow to be given a drench of any nature, as there seems to be danger from such procedure during and for twelve hours follow- ing a severe attack. The muscles of the neck and throat seem to be affected in these cases. A guarded prognosis must always be given in those cases which have been drenched before arrival of veterinarian. Catheterization should be performed on all animals which are in a comatose condition and also on those where the attack lasts to exceed six hours. Occasionally a case is met where the animal regains a per- 66 A. A. Mornrey fectly normal condition except a refusal to arise; patient eats, drinks and ruminates normally, and temperature, pulse and respiration are normal. Sometimes this continues for several days. One case of this nature, in my practice, crept around the yard eating grass and apparently well in every way, for a week, in spite of every effort to make her arise. Finally one morning the family found her walking around as natural as ever. This animal had been on a strychnin treatment during the entire time she was unable to rise. When this condition is met medical treatment seems of little use and mechanical methods no better, although it is well to continue the strychnin treatment. You may try for an hour or two to make a patient of this kind arise, and fail, leave the barn for half an hour, and return to find her up and acting as though nothing had happened. Upon examination of these cases after they had arisen I have never noticed any muscle atrophy or other condition that would lead me to believe that any pathological condition had caused the animal to remain down longer than the usual length of time. It is probable that muscular weakness is in a measure respon- sible for the condition. 7 In closing I will say that I have never seen a case in a primipara or following other than a normal birth, and never a case with retained placenta that the membranes were at all firmly attached to the cotyledons. Many cows suffer attacks each succeeding year; others never have a second attack, and some suffer an attack every other year. I recall treating one family cow four times in eight years. Hach time the animal presented a well-developed case of parturient paresis, the peculiar incident being that she was attacked every second year. No case is too far gone to respond favorably to proper treat- ment unless the patient is dead upon arrival of veterinarian. Due to the fact that the cause is still unknown, nothing has been successfully done along the lines of prophylaxis. (NoOTE.—Discussion of this paper will appear in the next issue of THE JOURNAL.) eS ly STRONGYLIDOSIS IN THE HORSE ' By C. H. Covauut Associate Professor of Veterinary Medicine, Iowa State College, Ames, Iowa STRONGYLIDOSIS is a febrile, enzootic disease of horses and mules which is characterized by enteritis, progressive ema- ciation and anemia, and caused by certain of the nematodes belonging to the family Strongylide. The disease is seen only in young animals from three months to five years of age, though the parasites are seen in horses of all ages. The condition has been described under the names sclerostomiasis and strongy losis, but the foregoing term seems preferable in the light of our pres- ent knowledge of the etiology of the disease. In the State of Iowa alone, hundreds of young horses have died from this disease in the past few years, and information reaching us indicates that the losses are as severe in other States and that it occurs in most of the States of the Union. It is especially prevalent in the States of the Mississippi Valley, having been reported from Minnesota to Louisiana. It has been reported from the Southern States and as far west as Montana. We can see no reason why the disease should not be found in all portions of the United States where young animals have access to wet, swampy pastures, as it is from such that infestation occurs. While our knowledge of the etiology of this disease may be lacking in certain details, we know that it is caused by certain nematodes of the family Strongylidx, hence the name strongyli- dosis. Of the many parasites revealed by postmortem examina- tion of animals dead from strongylidosis the most constant of- fenders and of first importance are the three species of the genus Strongylus, namely, S. vulgaris, S. édentatus and S. equinum. These three have been classified as belonging to the genus Sclerostomum, hence the name sclerostomiasis. Furthermore, both Strongylus vulgaris and S. equinum have been designated as S. armatus. 1 Presented at the fiftv-seventh annual meeting of the American Veterinary Medical Association, Columbus, Ohio, August, 1920. 67 68 C. H. Covauur It is from the standpoint of the practicing veterinarian that this discussion is entered into and I feel safe in saying that it is seldom that the practitioner so familiarizes himself with these parasites as to enable him to identify the different species, but the genus can usually be determined with a fair degree of accuracy. The strongyl, being blood-suckers, vary in color from a pale pink to either a bright or bluish red depending upon the amount of blood they contain. They vary in size from 14 to 214 inches in length and from the size of a thread to one-sixteenth of an inch in thickness, the size depending upon the age, sex and species. Probably the most common companions of the strongyli are the various species of the genus Cylicostomum, considered by many investigators in the past as being one and designated by some as Cyathostomum tetracanthum, by others as Strongylus tetracanthus, and by still others as Sclerostomum tetracanthum. It has been determined, however, that the small, threadlike white worms seen in such great numbers and commonly referred to as tetracanthus and as belonging to some one of the three genera just mentioned represent in reality at least twenty-two species of the genus Cylicostomum. In addition to these, Looss recognizes one species of the genus GYsophagodontus, G4. robustus; five species of the genus Triodontophorus, T. serratus, T. minor, T. brevicauda, T. intermedius and T. tennwicollis, and two species of the genus Gyalocephalus, G. capitus and G. equi. The part played by each of these in producing the disease is not known, but the greater part of the damage done is attributed to the three strongyli. It is quite generally believed that the life histories of the various genera of the family Strongylide herein mentioned are much the same. The adult parasites inhabit the intestinal lumen, where they lay eggs, which are voided with the feces. Under favorable conditions of temperature and moisture embryos are developed after a very few days. The embryos moult one or more times, and after moulting are very resistant and will live six to eight months in moist manure or in water. These embryos are taken into the digestive tract of the host with contaminated food or water, and either finish their life cycle in the lumen of the bowel or encyst themselves in the intestinal mucosa or sub- S@RONGYLIDOSIS IN THE Horse 69 mucosa until sexually mature, when they emerge, copulate and repeat the life cycle. When burrowing the embryos sometimes pass through the wall of the intestine and become encysted be- neath the serous membranes or in the lungs, liver or pancreas, or they enter the blood vessels. Larve of Strongylus vulgaris are commonly found in the mesenteric arteries or their branches, where they+ produce thrombosis and aneurisms. They have oc- casionally been found in the pulmonary, uterine and _ iliac arteries or their branches, where they produce thrombosis and aneurisms. They have occasionally been found in the pulmo- nary, uterine and iliac arteries, where they produce like lesions. Larve of S. edentatus are commonly found beneath the serous membranes, and those of S. equinum are commonly found in internal organs. It was formerly taught as a part of the life history of S. vulgaris that the larve developed in the blood stream until reaching a form resembling the adult, though with- out sex organs; at this stage it detached from the aneurism, passed to the walls of the cecum, where it became eneysted ; later the cyst opened into the lumen of the cecum, setting free the sexually mature worm. It is now believed by many that the larve in the blood vessels arrive there through accident and while they may reach sexual maturity do not reproduce. The injury to the host is probably due to the combined effects of the mature and immature worms. The constant irritation of the blood-sucking adults together with the irritation from the encysted larve leads to a chronic thickening of the intestinal wall, with a resultant loss of function, leading to digestive dis- turbances, as evidenced by diarrhea, ete. This hindrance to absorption from the intestinal tract, together with the loss to nutrition through the blood-sucking parasites, as well as the loss to the host from the absorption of nutriment from the intestinal lumen by those parasites not bloodsuckers, rapidly depletes the system of the undeveloped host, leading to emaciation and anemia. ‘ It is the belief of the writer that the presence of larve in the mesenteric vessels has little or nothing to do with the disease in question. It is true that this accident sometimes produces death through thrombosis and embolism, but clinical experience and postmortem findings do not indicate that this process operates to any extent in the production of the symptoms characteristic of 70 C. H. Covauut the condition which we term strongylidosis or sclerostomiasis ; therefore, consideration of thrombo-embolie colic does not seem advisable in this discussion. The mechanical injury to the in- testinal mucose by the adults and immature parasites opens up paths for infection leading to local or metastatic abscess forma- tion or enlargement and infiltration of the mesenteric lymph glands, resulting in an edematous condition of the mtestine, or it may result in general infection hastening death. Likewise, death may result unexpectedly from peritonitis and septicemia through the rupture of a subperitoneal abscess. Another factor, possibly of great importance in the produe- tion of symptoms and death, is the effect upon the host of toxins liberated by the parasites under certain conditions. In out- breaks where losses have been extensive and where deaths have been sudden and unexpected we have been unable to attribute the losses to any cause other than an intoxication. Postmortem ex- amination in such cases revealed no changes other than those in the intestinal wall, and no symptoms had been manifested until a few hours preceding death. It is known that many of the other parasites infesting the intestinal tract liberate toxins hav- ing a marked effect upon the host. With reference to the toxicity cf the strongyli the following abstract from the Journal of Agricultural Research is of interest :’ This is a biochemical study of the parasite S. equinuwm. From the results it is seen that this organism contains fats, fatty acids con- sisting chiefly of butyric acid, soaps, mucin, albumoses, purin bases and creatin, a ptomaine, a crystallizable alkaloid, an amino acid, and an amorphous substance containing a nonprotein nitrogen and phos- phorus which gives the precipitating reactions for an alkaloid. The cuticle of this parasite was also found to contain a unique protein combination which has no relation to chitin. Contrary to the findings of Weinberg, an alcoholic extract of the parasite was found to possess a strong hemolytic power. The soaps, fatty acids, crystallizable alkaloids, and ptomaines were active in the hemolytic process. The parasite further secretes a lipase which is capable of digesting the fat contained in horse serum. This lipase, according to the author, plays an active role in hemolysis. The bilirubin of horse serum is changed by the cellular activity of the parasite. Upon autopsy of the usual fatal cases of strongyloidosis one is attracted by the markedly emaciated, anemic cadaver. Often there is edema of dependent portions. Upon opening the ab- dominal cavity, cysts containing the larve, small abscesses re- 14 chemical study of Sclerostomum equinum. T. Bondouy. Arch. Par. 14 (1910), No. 1, pp. 5-39. aes SK. a :,!,.,T,CrCtC SUC SWRONGYLIDOSIS IN THE Horse 71 sulting from these, or nodules resulting from the healing process are noted beneath the peritoneum. Often small hematomata are noted in the peritoneum. The mesenteric lymph glands are sometimes enlarged, and the subperitoneal tissue in such cases shows an edematous infiltration with an amber-colored fluid. The walls of the cecum and large colon are thickened and sclerotic and contain cysts and abscesses. The mucosa shows innumerable small red spots due to the piercing of the mucosa by the blood suckers and is thickly studded with small reddened elevations which contain larve. Ecchymotic hemorrhages are commonly present in the different coats of the small intestine. The lumen of the intestine usually is filled with a wriggling mass of para- sites, millions of them being present free in the lumen and mixed with the ingesta and great numbers attached to the mucose. The embryos are ingested by the host during the late spring and summer and sometimes produce symptoms of disease as early as November or December. Usually in our locality the cases are called to the attention of the veterinarian during the first four months of the year, though it may be much later, and as seen by him can be divided into two classes, the difference in the two being only in the extent of development of the symp- toms. The interval between the ingestion of the embryos and the appearance of the symptoms as well as the severity of the symptoms depends upon the extent of the infestation and the resistance of the individual, the latter being determined largely by the feed and care the animal receives. Upon those farms where the feed and care of the colts is given proper attention, or in herds where the disease has previously existed and the owner is alert, the veterinarian is called early and given the history that the colts are unthrifty in spite of proper care and judicious feeding. If any of the affected ani- mals happen to be used for work it is further reported that these tire easily. Upon examination one finds signs of malnutrition, rough hair coat, leathery, harsh skin, and fair or poor condition of flesh. Quite commonly local sweating is observed, especially in the region of the flanks. Pulse, respiration, temperature, mucous membranes and appetite appear normal. Feces are usually normal, but in some cases there are indications of an intestinal catarrh, the feces being of softer consistency and malodorous, with the peristalsis active. 72 C. H. Covauur The second class of cases is characterized by the severity of the symptoms and are most often met with on those farms ‘ where the growing animals are allowed to ‘‘rough it’’ through the winter months, 7. e., feed in the cornstalk pasture with little or no shelter. They also occur under the best of hygienic con- ditions where the infestation is great or where for any reason the individual resistance is not marked. There is usually a history that the animal has lost flesh and strength rapidly; has a profuse diarrhea and marked thirst; appetite normal, in- ereased or variable. The principal symptoms are extreme emaci- ation, weakness and exhaustion. The hair coat is rough, bones prominent and the eyes sunken. The visible mucose are anemic and often edematous. Peristaltic sounds are loud, and a watery, fetid diarrhea persists which soils the tail and buttocks. If the patient is seen when still able to walk it is noticed to have a staggering gait especially noticeable in the hind legs. Often the veterinarian is not called until the animal is recumbent from weakness. The pulse is weak, thready and irregular, often inter- mittent. Edema of dependent portions is frequently present. The temperature is normal except where elevated from secondary infection. Where daily temperatures are recorded in the febrile eases the fever is found to be irregular or intermittent. In recumbent patients areas of decubital gangrene are seen. The course varies from two weeks to two months from the first appearance of symptoms, the variation being due to the factors mentioned as determining the character of the symptoms together with the extent of bacterial invasion of the traumatic lesions and the location and character of the accidental lesions produced by the larve. In the first class of cases the prognosis is good providing proper curative measures be early inaugurated. In the ad- vanced cases the prognosis must be guarded, since the mortality rate is sometimes very high. Death is usually due to exhaustion following a prolonged, progressive emaciation and anemia. It must also be borne in mind that death may come earlier from any of the following: (a) Thrombosis of the mesenteric arteries. (b) Peritonitis, due to intestinal perforation at the site of a verminous cyst or from rupture of a subperitoneal abscess. baie. el ~) we =, i STRONGYLIDOSIS IN THE HORSE (c) General infection taken up through the traumatic lesions in the intestinal mucose. (d) Septicemia from decubital gangrene following great weakness. (e) Internal hemorrhage from the rupture of an aneurism. Furthermore, recoveries are often incomplete, it being impos- sible for the severely injured intestinal wall to resume its normal functions. The walls are thickened, the circulation impeded, the mucosa contains much scar tissue, and absorption is interfered with permanently so that nutrition is impaired. There is chronic indigestion with constant or intermittent diarrhea, the appetite is Capricious and emaciation persists. Diagnosis is not difficult in districts where infestations are common. Features which lead to suspicion are: History of the patient having pastured on low, poorly drained or marsh land, or having been sufficiently exposed to animals known to have been infested; the enzootic character; the season of the year at which the symptoms appear; the gradual intensification of the symptoms as already outlined. Of especial diagnostic import- ance is the finding of the worms in the feces. When diarrhea is present the worms are easily seen in the freshly voided feces, or if the arm is inserted into the rectum the worms will be seen on the hand and arm when it is withdrawn. Errors are not un- common in cases where diarrhea is not present. The feces contain few or no worms. Rectal exploration may fail to reveal their presence. Where the history and symptoms indicate an infestation the diagnosis should be withheld until a purge has been administered and a reexamination made during the period of purgation, when in positive cases the parasites will be found in great numbers. It must be remembered that these parasites can be present in an individual without producing disease, so that the mere finding of the parasites is not of sufficient import- ance to exclude the necessity of further careful physical ex- amination. In our locality laymen who have had _ previous experience frequently make a diagnosis and call at the office asking to have their colts treated for ‘‘little red worms.’’ In those districts where infectious anemia exists the two diseases can be easily confused. It is reported that in infectious anemia there is a gradual increase in the temperature from day to day which might assist in the differentiation. 74 C. H. Covauut Treatment consists primarily in attempts to remove the para- sites and to build up the resistance of the patient. To remove the parasites from the intestinal tract we prefer a combination of tartar emetic and ferrous sulphate, 1 to 2 drams of the former and 2 to 4 drams of the latter, the dosage depending upon the age, given daily in capsule or on a small amount of feed until six doses have been given. This we prefer to give in the morn- ing before feeding. Unless the severe diarrhea or the weakness of the patient forbids, this six-day treatment should be preceded and followed by a purge. For this purpose aloes is to be pre- ferred. Leneveu recommends 2 to 5 drams of carbon bisulphide given in capsule for five days and followed on the sixth day with a purgative, preferably magnesium, sulphate. Turpentine in oil at intervals of three or four days has also given fair satisfac- tion. Thymol in ounce doses given after a period of fasting has, according to our limited observation, given only fair results. Oil of chenopodium given in from 14 to 1 ounce doses following 24 to 36 hours fasting and followed by an aloes purge is said to give good results. At the time of our first visit we administer 20 to 30 grains of atoxyl or 30 to 45 grains of sodium cacodylate intravenously, and repeat this dosage in ten days to two weeks. Rarely a third dose is given at the same interval. Where recovery is delayed we repeat the administration of tartar emetic, ferrous sulphate and sodium cacodylate after three weeks. While the great value of the preparations of organic arsenic can, not be denied, there is some difference in opinion as to how the beneficial action is exerted. It is stated that when given subcutaneously or intravenously they destroy the mature worms in the intestinal tract. Also that repeated doses destroy the en- eysted larve. The marked improvement following their use leads one to suspect that this may be true. It has been partic- ularly emphasized that the marked improvement is due to the destruction of the larve in the blood stream, and while this action probably takes place, and if so is of value in the end, it is difficult to believe that their destruction could have any appreciable effect on the course of the disease under discussion. Leneveu recommends sodium cacodylate because of its alterative tonic effect, and it is possible that the beneficial results come asa result of this action. a —ss 4 co | ~ STRONGYLIDOSIS IN THE HorsE Further tonie treatment consists in the administration of strychnine and imorganie arsenic in proper dosage. To allay intestinal irritation and control the diarrhea, salol, bismuth subnitrate or precipitated calcium carbonate should be ad- ministered. Attempt to combat heart weakness with subcutaneous injec- tions of camphor in oil or digitalis may be used per os. Complications must be treated as they appear. Decubital gangrene should be guarded against in the weakened patient by the use of slings. Marked weakness or threatened collapse is combated by the subeutaneous injection of from 1,000 to 2,000 mils of sterile normal saline solution. Of first importance is the allowance of a generous diet, and this phase of the treatment can not be overemphasized. In addition to the dietetic and medicinal treatment, prophy- lactic measures must be inaugurated to prevent infestation. Attempts should be made to secure proper under-drainage of all marsh land. Horses should be kept away from poorly drained pastures and care should be taken that.the hay be free from contamination. The drinking water should come from deep wells or should be filtered. Infested animals should be isolated and their quarters frequently cleaned and disinfected and pro- vided with clean bedding. Livestock improvement, sectional in many cases, has ad- vanced rapidly during the past two years through the influence of the nation-wide purebred sire campaign. Horsemen in the mid-western horse-producing ‘States have perhaps said less about their attainments than their cattle-breeding brothers, but they have been plodding along, getting their results just the same. Breeder’s Gazette. They were in a railway train and were discussing Dickens. ““Well.’’ said one, ‘‘John puts ‘Bleak House’ first and ‘Martin Chuzzlewit’ second.’’ ‘‘Excuse me, gentlemen,’’ said a husky voice from the seat behind. ‘‘I don’t know your pal, John, but you’re being steered. There ain’t no such horses runnin’.’’— Fort Worth Star-Telegram. BOTULISM IN SWINE AND ITS RELATION TO IMMU- NIZATION AGAINST HOG CHOLERA By Ropert GRAHAM Professor of Animal Pathology and Hygiene, University of Illinois, Urbana, III. THE pathogenic significance of secondary bacterial invaders in hog cholera has been a subject of investigation since the development and application of the Dorset, McBryde and Niles anti-hog-cholera serum and virus treatment. Notwithstanding the reduced mortality from hog cholera due to the widespread use of anti-hog-cholera serum, the loss in swine immunized against hog cholera has yet been a heavy drain upon the swine industry. Beginning in 1918 bacteriological investigations of swine diseases occurring in Illinois were projected at the Uni- versity Laboratory of Animal Pathology. The outbreaks com- ing within the scope of these investigations may occur fol- lowing the administration of anti-hog-cholera serum and virus (one week to 90 days), and untreated young pigs on infected premises may frequently be chronically afflicted. The possible relation of the serum and virus treatment to the losses in ques- tien has been suggested, though the exact connection of same has long been a matter of conjecture. The disease in question is marked by gross pathological changes in affected animals, which include a hemorrhagic in- flammation of the lymphatic glands with petechial hemorrhages in the serous and mucous membranes when accompanied by hog cholera. An interstitial pulmonary edema with eatarrhal pneumonia and pleuritic adhesions may be observed in some cases. The intestinal wall is thickened in the early stages of the enteric type of the disease, while circumscribed necrotic spots are visible on the serous covering of the intestine. In cases of long standing diffuse areas of diptheritic gastro-enteritis have been encountered. The latter lesion is more commonly observed in young pigs. The heart, kidney, and liver show varying degrees of parenchymatous degeneration. In etiologie studies a filterable virus test for hog cholera has generally revealed the presence of a low-grade hog-cholera virus, providing the animals have been previously immunized lord id 16 30TULISM AND HoG-CHOLERA IMMUNIZATION ia against cholera. In a few untreated herds, where the disease may assume a more chronie course, the filterable virus of hog cholera could not be consistently demonstrated by the usual methods. Since the virulence of hog-cholera virus is diminished by filtration, the negative results are subject to further study before final conclusions are formulated. Bacteriological studies of tissues from afflicted animals in 30 spontaneously ‘affected herds frequently revealed the presence of Bacillus suipestifer and related organisms of the paratyphoid group, while B. necrophorous was demonstrated in certain local lesions together with extraneous Gram-negative and_ positive spore-bearing rods. The latter tinctorially and morphologically resembled B. botulinus, while subsequent cultural, toxogenic and serologic tests confirmed the identity of this microorganism. One or more afflicted pigs were submitted from each herd. Ninety per cent of the outbreaks were related to B. botulinus type A. Moreover, B. botulinus type A has been frequently encountered in the surface soil of many of the infected premises. The syndrome observed in affected animals may vary in intensity, depending upon the age of the animal and the pres- ence or absence of hog cholera. In mildly affected animals following immunization, weakness or stiffness in the hind legs with knuckling of the pasterns may be noted. Unthriftiness and drooping ears followed by a variety of nervous manitesta- tions appear. Muscular twitching of the tail is observed in some animals. Occasionally a typical case of posterior paralysis or gluteal neurasthenia may develop. The breathing in advanced cases may be shallow with or without a thumping movement in the flank. Dyspnea and nasal catarrh give rise to a wheezing sound during inspiration. Conjunctivitis with a muco-purulent discharge may prevail in some animals and the eyelids become firmly glued together. Visual disturbances are commonly observed independent of inflammatory changes in the conjunctiva. : The sporadic syndrome briefly described may be reproduced by injecting hog-cholera serum and virus with varying amounts of botulinus toxin, while toxin injected per se in young pigs results in unthriftiness and malnutrition, with enteric and pul- monie disturbances analogous to those observed in pigs on naturally infected premises. 78 Rospert GRAHAM The not infrequent occurrence of the disease or diseases referred to above throughout the Corn Belt following immunization against hog cholera suggested its probable relation to the serum and virus treatment. In order to obtain bacteriological evidence on this point seventy-four samples of commercial hog cholera serum and virus were examined with special reference to anaerobic flora. Many different species of anaerobes were en- countered, but it seemed significant that 16 per cent of the samples purchased in the open market proved positive to B. botulinus type A. Of this number 10.8 per cent of the positive samples were hog-cholera virus, and 5.2 per cent were hog-cholera serum. ‘The serum and virus samples included in this series. were in many instances of the same serial number employed in the treatment of herds that developed illness subsequent to im- munization, and it is presumed from the preliminary evidence that a higher percentage of the commercial anti-hog- cholera serum and virus is contaminated with toxogenic strains of B. botulinus type A, while the possible significance of other pathogenic anaerobes has not as yet been eliminated from con- sideration. The possibility of botulism in sick herds following hog-cholera immunization should not be disregarded, though the filterable virus of hog cholera has been demonstrated in a major- ity of outbreaks. In the light of bacteriologic and serologic tests it is apparent that hog cholera is the predominating disease in post-vaccina- tion illness in swine. The incubation and development of cholera in recently immunized herds in our judgment may be traceable to the fact that the serum and virus treatment is simul- taneously superimposed upon a specific botulinus intoxication which lowers the resisting powers of the animal and permits the development of a latent and low-grade cholera. Aerobie pathogens such as Pasteurella suiseptica and several unidentified saprophytes in the tissues of afflicted animals do not appear to be a significant or primary factor in the losses. In fact the administration of bacterins prepared from the hemor- rhagic septicemia group have been found not only worthless, but detrimental in preliminary experiments. On the contrary the injection of botulinus antitoxin type A in so-called ‘‘breaks”’ following immunization, in preliminary trials under field condi- tions, is highly efficacious, providing hog-cholera virus infection a FIOM Mi! ore £ + Oy 08 et ert ee ae BorunisM AND HoG-CHOLERA IMMUNIZATION 79 is eliminated or subjected to control by the administration of a second dose of anti-hog-cholera serum. The logical procedure to be tentatively recommended in avoid- ing loss subsequent to immunizing hogs with commercial anti- hog-cholera serum and virus as at present prepared, consists of a prophylactic injection of botulinus antitoxin (500 units) to offset the specific intoxication which may be produced by the use of serum and virus, until a better means is devised to elimi- nate all possibility of infected serum and virus. Furthermore, the relation of a food intoxication which might result from con- taminated rations fed incident to immunization further justifies _this prophylactic treatment. On infected premises pigs should be treated before symptoms of unthriftiness and malnutrition appear, since the curative effects of the antitoxin are limited. Chronie cases which at autopsy show well-organized secondary and nonspecific lesions of enteritis are amenable only to early treatment with botulinus antitoxin. On the contrary, bacterins of the hemorrhagic septicemia group, sometimes recommended in the treatment of such conditions, have proven detrimental in preliminary experiments. Dr. L. M. Buffington has been transferred from the Govern- ment tick-eradication force in Arkansas to hog-cholera work in Iowa. Dr. W. M. MacKellar, who for some time has directed the Bureau tick-eradication work in Georgia and Florida, has been transferred at his own request and assigned to the position of inspector-in-charge of field work and hog-cholera control in Cali- fornia, with headquarters at Sacramento. Dr. S. J. Horne as- sumes the duties formerly performed by Dr. MacKellar, with headquarters at Atlanta, Ga. Doctors J. R. Wirthlin and A. M. Kirkpatrick have resigned their positions on the tick-eradication force of the Bureau of Animal Indutsry in Georgia. Dr. Edward Horstman, of the Federal tick-eradication force, | has been transferred from Louisiana to Georgia. Dr. George M. Paxton is now acting inspector-in-charge of this work in Louisiana. A TRIBUTE TO DEAN W. HORACE HOSKINS By Wiw1AM Hersert Lowe Paterson, N. J. FORTY years ago William Horace Hoskins was graduated from the American Veterinary College, New York City. He was then twenty-one years of age, possessed of an indomitable will and a high purpose. He had learned how to study and had been inspired to greater things. It was at the feet of Liautard and his confreres that Hoskins commenced his notable career. Ability, industry and loyalty were outstanding characteris- tics of the man. He was a remarkable leader in the profession. As an organizer, local, State and national, his achievements are unparalleled in the annals of the veterinary profession in America. In the upbuilding of the American Veterinary Medical Asso- ciation, which organization has become the largest and most influential veterinary association in the world, Hoskins played a most conspicuous part. He served the association as secre- tary for a time and later as president for a term of three con- secutive years. He enjoyed the unique distinction of never having missed attendance at an annual meeting of the American Veterinary Medical Association and also of the Alumni Asso- ciation of his Alma Mater, during the entire forty years of his professional activity. From the day of the organization of the Federal Bureau of Animal Industry, in 1884, until the day of his death, he was a pillar of strength to that great department of our national government. The present high status of the veterinary service in the United States Army is the result of twenty-five years of arduous labor on the part of our deceased brother who was largely in- strumental in placing an act of Congress on the statute books in 1916, providing for commission and rank for veterinarians in the United States Army. To recount his great work in his native State of Pennsylva- nia would be a task beyond the limits of the present hour. Leonard Pearson and W. Horace Hoskins were indeed a team s0 Tripute To W. Horace HOsKINs S1 that has not been matched since the death of the former and great were the achievements for the University of Pennsylvania and for the Commonwealth. Dr. Hoskins served the State Board of Veterinary Medical Examiners of Pennsylvania as secretary and executive officer for sixteen consecutive years. He was honored with the presi- deney of the Pennsylvania State and Keystone Veterinary Med- ical Associations, and also held the professorship of veterinary jurisprudence in the University of Pennsylvania for. many years. He was an honorary member of the vetermary associa- tions of a number of States, also of other scientific and frater- nal organizations. Our brother distinguished himself also in the field of journal- ism. He became editor-in-chief of the Journal of Comparative Medicine and Veterinary Archives, the lamented Rush Shippen Huidekoper being his associate in his editorial work. Dr. Hoskins was a convincing and brilliant public speaker and has been referred to by his friends as the ‘‘silvered tongued orator’’ of the profession. ‘He was not a laboratory man or research worker, but he had the faculty, developed to a remarkable degree, of being able to appropriate and utilize the discoveries made in the laboratory to the advancement of the profession and the uplift of mankind. A biographical sketch of Dr. Hoskins has been given a place in ‘‘Who’s Who in America’’ and also in ‘‘ American Men of Science.’ Four years ago, upon the death of Dean William J. Coates, Dr. Hoskins succeeded to the Deanship of the New York State Veterinary College at New York University, whereupon he moved from Philadelphia to New York. His wonderful work here is so recent and familiar to us all as not to need review at this time, but let it be recorded that in placing veterinary edu- cation at its birthplace on this continent upon a more substan- tial and enduring basis than it had previously occupied, he performed a Herculean task which has been highly commended by true lovers of a noble profession. Dean Hoskins is survived by his wife Annie E. (née Cheever ) and a daughter, Mrs. Margaret E. Hofstetter of Philadelphia; also two sons, Dr. H. Preston Hoskins, Detroit, and Dr. Cheston M. Hoskins, Philadelphia, both of whom are veterinarians, and §2 WILLIAM HersBertr LOWE seven grandchildren. He is also survived by a brother, Pro- fessor J. Preston Hoskins of Princeton University. We would extend loving sympathy to his family in their bereavement. He was a God-fearing man of splendid character, respected and esteemed. The foundation stone of his monument is already built in this college, which was so dear to his heart. The veteri- nary ficulty of New York University has lost its distinguished Dean and the veterinary profession of the State, the Nation and the world, one of its strong men. A London cable to the Philadelphia Ledger says: ‘“The royal commission appointed to consider the removal of the embargo upon Canadian store cattle, or cattle for slaughtering purposes, reported September 15 that the removal would tend to increase the meat supply of the country and lower the price. The report expresses the opinion that Canadian cattle are healthier than Irish cattle or British cattle. As to the danger of diseased cattle crossing the border from the United States into Canada, the commission says: ‘There is no record of anything of the kind at any time. Canadian cattle are all branded with dis- tinetive marks, and with ordinary care there is little chance of United States cattle being sent to this country as Canadian cattle.’ ’’ _American Hereford Journal says: ‘‘The purebred-sire cam- paigns carried on by Federal and State authorities, livestock associations and breed papers have shown to the farmer the value of purebred sires in their herds, and have created a desire for ownership. Now that the prices of good sires are greatly reduced, these desires can be gratified. The way is prepared and the field is large.’’ Dr. J. W. Etheridge, who has been on hog cholera work in North Carolina, has resigned from the service of the Bureau of Animal Industry, effective September 15. Dr. F. H. Schleich, formerly of the Federal hog cholera force in Tennessee, is now doing meat inspection work at Chicago, Il. "= EXPERIMENTS TO DETERMINE THE VALUE OF CHICKEN-POX VACCINE ° By FrRep Boerner, Jr., and E. L. Srupps Philadelphia, Pennsylvania. THE EXPERIMENTS herein recorded were carried out by the Pennsylvania Bureau of Animal Industry, first, as the re- sult of numerous inquiries from poultrymen regarding the value of chicken-pox vaccine or bacterin, and second, of our failure to find anything in the lterature which could be considered as conclusive proof that such products were valuable and worthy of recommendation. The works of Manteufel (1), Hadley and Beach (2) and Mack and Records (3) fail to furnish the proof that we desired. Since the beginning of our work Beach (4) has reported ‘further investigation upon this subject. He found that a vac- cine was valuable as an adjunct to other remedial measures. One experiment was cited in which 168 diseased fowls were vac- einated and given local treatment, and a like number left as controls which were given local treatment but not vaccinated. Of the vaccinated the mortality was 19.6 per cent and of the controls 39.9 per cent. The average number of days required for recovery of the vaccinated fowls was 10.8 and of the con- trols 13.7. He further states that the vaccine is designed for and chiefly used as a preventive, and following experiments carried out in California in 1914 its use was recommended to commercial poultrymen. In one year over 300,000 birds were vaccinated in California with vaccine prepared by Beach. A series of ex- periments was cited in which 1,026 healthy fowl were used, 900 vaccinated and 126 left as controls. . They were then exposed to infection, with the result that 11 per cent of the vaccinated and 86 per cent of the controls became infected. Beach’s method of preparing the vaccine is different from that of previous workers in that only scabs are employed, these being obtained from artificially infected fowl. The scabs are ‘Presented at the fifty-eighth annual meeting of the American Veterinary Medical Association, Denver, Colo., September 5-9, 1921. 7) do s4 FRED BoERNER, JR., AND E. L. Stuns thoroughly dried and then powdered. One dram of the powder is diluted with 100 mils of physiological salt solution, and of this 1 mil represents the amount to be injected. Our first attempts to prove the value of chiecken-pox vaccine were carried out during the spring of 1920 in two large, badly infected flocks. The vaccine was prepared according to the method employed by Hadley and Beach and which was also used by Mack and Records. The heads of badly: affected chickens were brought to the laboratory, where the scabs from combs and wattles, the pseudo-membranes from mouth and throat and the cheesy collection around the eyes were removed, finely minced and triturated with a small amount of normal salt solution. The mass was allowed to macerate in the ice-box over night. It was then filtered through cotton, diluted with salt solution, and finally heated to 58° C. for one hour. EXPERIMENT No. 1. The farm, located in York County, Pennsylvania, was visited , March 17, 1920, at which time the flock was found to be badly infeeted with chicken-pox and the mortality high. The owner stated that it was the first outbreak of its kind he had had and that the flock had been suffering from it the greater part of the winter. He was willing to allow us to vaccinate a portion of the flock and leave the remainder as controls. Heads showing marked lesions were collected and brought back to the labora- tory for the preparation of the vaccine. ; As shown in Table 1, the birds were kept in four houses, num- bered respectively 1 to 4. In houses 1 and 2 there were three pens each, designated by the letters A, B and C, while houses 3 and 4 contained but two pens each . During the week following the original visit all the fowls in house No. 2 and one pen in house No. 3 and one pen in house No. 4 were vaccinated. One pen in each of houses 3 and 4 were left as controls. On this same date one pen in house No. 1 was given a second injection, and on April 1 one pen in house No. 3 was Injected the second time. ; In the course of the experiment the owner kept records of the number which died in the various pens and a record of the egg production for 21 days subsequent to vaccination. On May 19 we visited the premises and found a marked im- ‘3 f VALUE OF CHICKEN-Pox VACCINE 85 provement, there being but few birds affected. The control pens appeared to be in as good condition as the others. We have summarized the results in Table 1, a study of which ean allow but one conclusion, and that is that the vaccine was not demonstrated to be of any value. The best control we had was in case of house No. 4, where the number of affected and healthy birds were in about the same proportion in both pens and the results were practically the same in both, whether vac- cinated or not. It is to be noted that in house No. 1, pen C, and house No. 3 pen A, where the fowls were given two injections, the mor- tality was very much higher. This was probably due to the fact that these two pens were in bad condition when first treated, having a larger number of affected birds than the other pens. The high mortality also influenced the egg production per hen. TABLE 1.—ReESuLTS OBTAINED IN ExPEeRIMENT No. 1. | Date of | Date of Mortality Percent Egg pro- Eggs Number | Number | first vac- second | subsequent)mortality * duction | per House | Pen! affected | healthy | cination | Vaccina- to vacci- | subject to 30 days) hen tion nation vaccina- | | | tion ——— ee se eee Ale — =. | S | A 44 57 | Controls) Controls 12 11.8 1,527 14 1 B 50 22 B= 20-20) rane 11 P52. 993 13+ (C: 63 35 3-20-20 | 3-27-20 | 30 30.6 737 ti! A 45 See | bse 7-20 (etre se 9 8.7 2,460 | 13+ 2 B 16 52 79 EPA al ae eee 10 14.7 BAN ete ( 12 77 Waar =2ON | eoceet che, « 5 aye 1,343 Loo A 67 23 3-27-20 | | 4- 1- 20" 55 61.1 811 9+ 3 B 26 69 | Co ONCLOIS|p ye era 26.3 1,166 13+ A 20 65 | Controls|........ 12 14.1 1,051 12+ fe B 29 pA S-2 7-206 | 10 11.3 1095 | 12+ | | | i a Mortality includes all deaths Pom Mare h 20° to May 19. EXPERIMENT No. 2. This flock was located on a farm in Luzerne County, Penn- sylvania. The disease appeared during the month of April. On May 13 we visited the farm and arranged with the owner to vaccinate a portion of the flock and collected material for the preparation of vaccine. c On May 20 we returned prepared to carry out the treatment. The flock was kept in eight different houses, and of these three were picked as being the worst affected and were selected for treatment. Each bird was injected subeutaneously with 1 e.e. of the vaeeine. The houses which contained those treated were S6 FRED BOERNER, JR., AND E. L. STUBBS No. 3, in which 178 were healthy and 333 affected; No. 4, with $2 healthy and 99 affected; and No. 6, with 136 healthy and 200 affected. Those in houses 1, 2, 5, 7 and 8 were left as controls. The results of this experiment are shown in Table 2, and it is to be noted that the mortality for three weeks subsequent to vaccination was about one-half that for the three weeks prior to vaccination in both groups. This warrants the belief that the use of the vaccine in this outbreak certainly did no harm, but it is questionable if it was of any value as a means of checking the disease. The egg production was practically the same in both groups. . Taste 2—Monrrtatitry AMONG Fow1s IN ExPpEnIMENT No. 2. Weeks Ending House No. 5-8-20 | 5-15-20 | 5-22-20 | 5-31-20 | 6-8-20 | 6-15-20 | 6-23-20 | Total 1 5 42 25 13 3 9 8 105 2 1 5 13 10 1 een 35 = 14 15 39 25 3 2, 8 106 4* 8 6 19 De | rime berate 1 48 5 1 2 3 5 i el ee See 9 21 6* 9 28 39 29 2 3 2 112 ie POR Seca hee see Ane al EEE Se Fay 5| [SE es ee Seen Beene 2 8 ei! il AEE OOS 2 adil ec neee Ser 4 10 * All fowls vaccinated May 20, 1920. TABLE 3.—SuMMARY OF RESULTS OF ExPERIMENT No. 2. : Mortality for the 3 weeks! Mortality for 3 weeks? sub- House | Number of | prior to vaccination | sequent to vaccination No. Fowls | Treatment : Number Percent | Number Per cent 1 373 None | 72 16.18 25 6.97 2 490 = 23 4.48 2 2.44 5 589 ie 6 1.00 6 .98 7 632 is Be a) Meet eT SIS) (a Se LS 8 408, a ries | 97 | 2- 49 Total.. DGC >| ae 107 Aaa 45 1.80 Vaccinated 3 438 May 20, 1920 68 13.43 | 30 6.84 4 181 = 33 15.42 14 Tess 6 335 Ms 75 18.29 34 10.15 Total. | i Se 176. eal eee 78 8.17 1 Percentage based on number of fowl in flock on April 30, 1920. 2 Percentage based on number of fowl in flock on May 20, 1920. EXPERIMENT N6. 3 It was not until the following winter that an opportunity was afforded for the continuance of this work. Beach having re- ported his results during the summer, it was decided to employ the same vaccine that he recommended. Sufficient virus for this work was purchased from the University of California. The 2 VALUE OF CHICKEN-POX VACCINE 87 vaccine was prepared from this virus and injected according to the method recommended by Beach. The first flock in which this vaccine was used was in Dela- ware County, Pennsylvania. The disease had appeared on this farm in the winter of 1919-20. During the fall of 1920 a few cases developed, but the disease did not spread. The manager was able to control it by local treatment. The fact that these cases developed without a tendency to spread led us to question the diagnosis. Inoculation experiments were carried out and it was found that the disease could be readily transmitted. In November the fowl were housed and by the first of December the manager informed us that the disease was spreading rapidly and he was unable to control it. Arrangements were made to carry out an experiment in one of his houses. This house was divided into five pens. The two end pens, A and E, and one-half of the middle pen, C, were vaccinated December 7, 1920. Pens B and D and one-half of C were left as controls. No treatment other than the vaccine was attempted. On December 14 pen A was injected the second time. Each fowl was leg banded, thoroughly examined, and a record made of its condition at the time of vaccination. They were all carefully examined on December 20 and again on January 3. TaBLe 4.—ReEsutts or ExpenimMent No. 3. Observation | Observation Observation Description Condition 12-7-20 12-20-20 1-3-21 Pen A,* vaccinated twice Healthy........ 46 47 34 | Affected... |... 32 29 40 Pen B, controls realty... i-2 46 41 23 = Affected........ 34 | 39 51 Pen C,* vaccinated Mealthyt a... 16 19 15 7 fettected. 2.1. 19 12 19 Pen C, controls Healthy. <....<. - 18 i= 22 13 < Aviected. 622° 19 | 12 19 Pen C, controls Elealthy; 52.22. 18 22 13 Wedfected 52: < = ~:+- 19 15 24 é Pen D, controls Rlealthy. «3751. 51 47 = 35 Affected........ 25 29 39 Pen E,* vaccinated Healthy........ 52 52 32 | aflected..°.-.... 21 21 1 SumMMARY: = Vaccinated | Healthy........ 114 118 81 By | Affertedins <:... . + 12 62 100 Controls iptfealthye.-. 2. . | 115 110 7 7 | 83 114 Adrectetl: 22!%.. :. -78 *Vaccinated 12-7-20. &8 FRED BOERNER, JR., AND E. L. STUBBS Table 4 shows the condition of both the vaccinated birds and the controls on the above-mentioned dates. It will be noted that the disease remained practically sta- tionary for the first two weeks following vaccination. During the second two weeks it increased, as shown plainly in the sum- mary of results. It would appear at first glance that the vaeci- nated fowl showed some resistance, as on January 3 there were 81 healthy in the vaccinated lot against 71 healthy in the controls. A second glance, however, will show that there were more cases developed between December 20 and January 3 in the vaccinated lot than in the controls. The mortality in this flock was relatively low and is of little value in determining the effects of the vaecine. The followmg is a record of all deaths from December 7, 1920, to June 1, 1921: Nacemated birds. 9e 3224 Pens A, C and E, 21 CTE 0 5 eee See SB eee ee Pens B, C and D, 32 These numbers include all deaths, many of which were due to intestinal disturbances. During the spring enteritis was quite prevalent in this flock and was the cause of death in many in- stances. The number of those which died showing lesions of chicken-pox was: Vaccinated birds, 11; controls, 7. EXPERIMENT No. 4 Experiment No. 4 was carried on at about the same time as No. 3 and on a farm situated in the same county. The data are given in Table 5, from which it will be seen that the infection appeared in two houses, Nos. 1 and 2, in which there were nine pens in all, and in six out pens. The three columns give the conditions as found at the three different dates, it being understood that such pens as were vac- cinated were vaccinated December 10, 1920. It is to be observed that at the time of the first observation a certain number of birds, those vaccinated, and the controls, were both affected and healthy. At the time of the second ob- servation there had been a marked improvement, the number of infected birds being markedly fewer. At the time of the third observation, conditions had grown worse, which may probably be credited to an intervening spell of bad weather. At the time of the third observation the condition with regard to the controls was much as it had been originally, while there VALUE OF CHICKEN-POX VACCINE 89 TasBie 5.—Resurts Ontainep in Exreniment No. 4. ‘Description Condition Observation Ist Observation 2nd Observation 12-10-20 | 12-29-20 1-12-21 House No. 1: Pen A,* vaccinated Healthy... ._. 35 61 61 gan ectea <>; 38 12 12 Pen B, controls Healthy........ 36 47 42 ieratiected >... | 21 10 16 Pen C,* vaccinated Mealthy:.... =. 29 40 36 Affected........ 20 9 13 Pen D, controls | Healthy... _.. ‘ 18 35 59 Affected... =... 35 18 14 Pen E,* vaccinated | Healthy.\..-.... 28 | 43 34 Affected........ 19 4 | 13 Pen F, controls — Healthy........ | 29 40 29 , Affected........ 17 6 17 ~ House No. 2: pa Pen A,* vaccinated © Healthy... 0. ... . 56 58 4] Affected........ i! 5 22 Pen B, controls | atealthy: 2: s.4:,: 84 82 42 Mifected S02.) 3: 5 7 4 Pen C,* vaccinated Healthy:\..2/..... 57 54 50 4 Affected... ...... 6 9 13 Yn ee Out PENs: . No. 1,* vaccinated Medithiyin. . =... 10 20 21 a Peeoitected (252. ! 13 3 2 ; No. 2, controls Healthy: *....... 10 14 17 ieeeottected.. 0... 12 8 5 No. 3,* vaccinated | Healthy... .. 4 16 17 Peepottected: {552 ...,. 8 6 5 h No. 4, controls | Affected....-... | 12 18 14 No. 5,* vaccinated | Healthy: 52:45: 52 9 17 17 Affected....... 15 7 7 j No. 6, controls | Healthy... ... : 12 17 15 Adtectedc 2.55. : 9 1 6 7 SuMMaAny: - Y Vaccinated Healthy;...... 52... 238 309 277 t Affected........ 126 55 7 Controls Healthy! 2.045" 201 253 198 Affected 107 55 110 * Vaccinated 12-10-20. Was an improvement with the vaccinated birds. It is somewhat questionable, however, if this was due to the vaccination. If so, : it was not sufficient to be of practical value, as neither the bs egg production nor the mortality was favorably influenced. In % this experiment but two birds died, one of which had been vac- cinated and the other had not. These were not listed in the - table. Egg production, recorded from December 9, 1920, to January 11, 1921, inclusive, was as follows: 90 FRED BoERNER, JR., AND KE. L. Stupss Vaccimated. fowlsti) 22 see 8.38 cges per hen Controls eee 8.25 eggs per hen DIscussION These flocks were carefully selected as showing good typical lesions of chicken-pox, not complicated with lesions which might be confused with other diseases of chickens. In each flock there were numerous eases of small wart-like epithehal tumors on the comb, wattles or skin of the head, and these lesions are accepted by most authorities as diagnostic of chicken-pox. In addition to these external lesions there were also many diphtheritic areas or caseous and cheesy areas in the mouth, on the tongue and in the throat. All birds were handled at the time of vaccination and at each examination to determine the general condition and marking, the degree of affection of the comb, wattles, face, mouth, tongue and throat. In all the tables the birds marked affected showed either the small wart-like epithelial tumors known as external lesions or the diphtheritic or caseous areas on the tongue, in the mouth or in the throat. Those marked healthy are birds which were in good physical condition and showed no lesions of any kind. In Experiments 1 and 2 the disease had been present for some time, and if all the birds had been treated it might have led us to think that they were benefited by the treatment. The disease entirely disappeared from each of the premises and up to the present time has not been found again. In Experiments 3 and 4 if all had been treated and no physical examinations made it might have seemed that the disease had been checked. It is our hope to do more work along these lines, for up to the present time the results of our experiments, which were of fair magnitude and conducted in the field, do not confirm the results obtained by other investigators. In our hands the vaccine recommended and produced by Beach has apparently not been of practical value in controlling chicken-pox. It is of course possible that further studies will necessitate some modification of our conclusions, but since the whole matter is one of consid- erable importance, we felt that we were justified in presenting the results already obtained. We further hope that other in- vestigators may be stimulated to carry on similar experiments, so that absolutely definite conclusions may be reached as to the value of this product. 1 Aan Sal $e Asta Magy lala ate 4 ah «) ; 4 > | 4 4 VALUE OF CHICKEN-Pox VACCINE )] _ The authors wish here to express their appreciation to Dr. George H. Hart, of the Veterinary School, University of Penn- sylvania, for the valuable assistance given them in connection with Experiments Nos. 5 and 4. LITERATURE 1. MANTEUFEL. Arb. Kaiserl. Gesundh., 33 (1910), 305. 2. HADLEY and BEACH. Proc. Amer. Vet. Med. Assoc., 1913, p. 704. 3. Mack and Recorps. Bul. 82, Agr. Expt. Sta. Univ. of Nev., June, 1915. 4. BEACH. Jour. Amer. Vet. Med. Assoc., vol. 11, No. 3, p. 301. ANIMAL EXPERIMENTATION, THE DOCTOR AND THE DOG (From an article entitled “ In the Time of Henry Jacob Bigelow,” by William J. Mayo, M. D., Rochester, Minn., in Journal of the Amer- ican Medical Association, August 20, 1921.) JENNER on a certain occasion presented arguments which were capable of being proved, but which he had not proved. The following day John Hunter wrote to Jenner saying, ‘‘ Why submit hypotheses? Try it on a hedgehog and know.’’ Animal experimention has resulted in gifts of inestimable value vo humanity. The pernicious activities of antivivisectionists seri- ously threaten the continuance of these investigations, which are of such paramount importance to the nation’s health. It is of interest to note that in the last election in California the anti- vivisectionists, aided by various organized cults, such as Christ- ian Science, osteopathy and chiropractic, were defeated two to one by a referendum directly to the people, a triumph of an appeal to reason. The defeat of the antivivisectionists was owing in a great measure to the sagacity of Dr. Ray Lyman Wil- bur, President of Leland Stanford University. The people, the farmers in particular, were shown what animal experimentation has accomplished in the prevention of disease to farm animals, what it has accomplished for the canning industry in relation to botulism, and the possible effect on economic conditions in Cali- fornia if the work should be discontinued. It is undoubtedly, true that much of the opposition to animal experimentation among reasonable persons has been brought about by a few careless physicians. For at least four thousand years the dog has been man’s friend and companion, and the 92 ANIMAL [EXPERIMENTATION occasional buying of stolen family pets at small prices for animal experimentation, as has been done in some laboratories, has alien- ated the public. The physician should not be a fence for stolen property, and his complicity can not be glossed over by saying that he did not know the property was stolen. The physician has no more right to a stolen dog than to a stolen purse. All these difficulties could be overcome by a proper law which would turn vagrant dogs over to accredited institutions. At the present time thousands of homeless dogs, many times more than the number used in all the laboratories of the United States, are killed at the pounds of the various cities, often by methods entailing more pain than any experiment carried out in a controlled laboratory, and are an economic loss except so far as their hides and fat can be used for commercial purposes. Some methods should be devised whereby these animals, or as many as necessary, may become legally available for the use of recognized, responsible laboratories for experimental work. Moreover, the medical profession in the past has been derelict in permitting untrained men to perform animal experimenta- tion without supervision. It was such experimentation that roused the resentment of a great many persons. If it is the wish of the medical profession to continue this work, its members must not furnish a market for the thief and they must protect the dog from the untrained experimenter. Fortunately, the organized physicians of the country have already made con- siderable progress in controlling the conditions of animal experi- mentation from within. Regulations which place upon labora- tory directors responsibility for the importance of the problems studied and for the propriety of the procedures used in the solution of these problems have been formulated and adopted by corporate vote in medical institutions throughout the land. By pursuing the policy of honesty and faithfulness to a trust, animal experimentation can be carried on without serious objection. Experiments on animals were performed in Bige- low’s time, but today such investigation has become one of the foundation stones of progress. Dr. Emlen Wood has resigned from the position of Assistant State Veterinarian of South Carolina, and has started practice at Greenwich, Cumberland County, New Jersey. ' $ CLINICAL AND CASE REPORTS (Practitioners and others are invited to contribute to this depart- ment reports of unusual and interesting cases which may be helpful to others in the profession.) PYOSALPINGITIS AND THE SURGICAL TREATMENT THEREOF By G. E. JORGENSON Clermont, Iowa Patient.—A four-year-old purebred Jersey cow. History.—Had been bred and conceived when at the age of three years, the pregnancy terminating in parturition after a normal and uneventful duration. The birth, however, was quite pathological, presenting both a maternal and fetal dys- tokia as follows: There was present an anterior presentation in the dorso-pubie position with a lateral deviation of the head in the right ilio-cephalic position with both front feet engaged in the pelvis. Repulsion and version corrected this, and delivery of a live, well-developed calf followed. This calf developed acute enteritis and pneumonia within twenty- four hours in spite of the fact that it was not allowed to re- celve any nourishment and had been given several enemas to clean out the digestive tract and one dose of calf-scour serum. It died in two days. Cultures from the lungs and intestines showed two streptococci, one of the viridans group and the other a green-producing, hemolytic, long-chained variety. The cow revealed extensive placentitis and metritis with retained placenta and considerable uterine discharge. No at- tempt was made to irrigate the uterus, but instead capsules containing zine oxide and iodoform were placed deeply within its cavity every three days. In seven days the placenta was removed, and the uterus showed envugh involution in’ four days thereafter so that it could be douched and the fluid siphoned out. After about four weeks’ treatment the uterus and cervix were so nearly normal that the patient was dis- charged from treatment. Three months after parturition I was called to see her again for the reason that she had shown no signs of estrum. Vagi- 93 4 CLINICAL AND Cast Reports nal examination revealed a normal cervix firmly contracted, with no signs of any inflammation or discharge. Rectal ex- amination revealed a cystic corpus luteum in the left ovary. It is an established fact that cystic degeneration of the corpus luteum indicates an infection of the oviduct at time of rupture, which points to tubal infection on the side where present. Hence, for fear that rupture of the cyst at this time would further infect the adjacent tissues, treatment was confined to a careful dilatation of the cervix and irrigation of the uterus with normal salt solution. A small amount of flocculent muco-pus was siphoned out. Irrigations were e¢ar- ried out once a week for three weeks, at which time the writer was called away and was absent for three weeks. Upon his return the patient was examined and showed an extensive enlargement of the left tube with considerable adhesion of the pavilion to the ovary. The right ovary was normal, as was the tube as far as palpation could indicate. A diagnosis of pyosalpinx was made, and as the breeding efficiency of the animal was nil in the present condition, surgical interference was advised, cause for such action being based upon the radi- cal treatment of pyosalpinx in women. The operation was carried out as follows: a The animal was given 114 ounces of chloral after being dieted for twenty-four hours. ‘One hour after the adminis- tration of the chloral the animal was secured in the stocks in such a manner that it could not lie down, and an area twelve inches in diameter on the left side was shaved and cleansed in the usual surgical manner, after which an in- cision was made through the skin and underlying fascia down to the abdominis externus as far back as possible and about eight inches in length. Following this. the external abdominis obliquus ‘was divided in the direction of its fibers, while the internal was incised with the scalpel in the same direction as the external. The left tube and as much of the cornua as could be brought out were then exposed through the in- cision and the ovary and tube freed from the suspensory hgaments. Following this the tube was clamped off at its uterine termination and severed from its attachment thereto, after which the stump was ecauterized with pure phenol and a purse-string suture inserted in the serous coat and the tip ee ; 4 i y q : z i f el | aries CLINICAL AND CASE REPORTS Qh or stump pushed in, similar to the technique employed in an appendectomy on the human. The parts were then returned and the laparotomy wound sutured. The case made an un- eventful recovery and is now pregnant. Discussion.—Pyosalpinx is in the majority of cases bilateral. In fact, out of some forty cases seen by the writer this is the first one which appeared to be unilateral. If bilateral, surgical intervention is of course contra-indicated. In such cases it is better to leave the tubes alone and concentrate the treatment on the cervix and uterus in the hope that the tubal infec- tion may not prove so severe that occlusion occurs. The writer has found that a cystic degeneration of the corpus luteum serves as a fairly constant indication to tubal infection, and this is corroborated by other writers. In this particular case there was a spread of infection by contiguity from the uterus to the tubes, and while the cervix and uterus appeared to be freed from infection and inflammation, there however did exist a chronic salpingitis and to a lesser degree pyo- metra. The chronic salpingitis terminated, as it occasionally does, in a tubal abscess. Sections of the uterine end of this tube revealed a complete destruction of the epithelial ele- ments and a replacement with connective tissue, resulting in a complete occlusion of the tube, which extended to within two inches of the tubal abscess. This abscess involved the pavilion, which was entirely adherent to the ovary, which Was involved in the abscess. In this particular case there is another feature of interest. There was in this herd a chain of evidence running back to the bull. The bull came from a herd where genital infection had been rampant, and was finally discarded himself for the reason that he suffered from a partial sterility which grad- ually became absolute. Examination of this bull showed sev- eral pathological features, as. follows: There was present an oligospermia. There were spermatozoa that showed various imperfections such as absence of tails, absence of nuclear membranes or atrophic nuclei and lack of motility, together with the presence of streptococci in the semen. Clinically an enlargement. of the seminal vesicles and atrophic degenera- tion of the left testicle could be demonstrated. Autopsy on this bull later showed a chronic semino-vesiculitis and an atrophic degeneration of the testicles. Later it was learned OG CLINICAL AND Cast REPoRtTS that this bull had suffered from scours during calfhood. The cow herein deseribed had been bred to this bull and the calf born in dystokia and later dying from septic enteritis and pneumonia was its progeny. Cultures from the ampu- tated tube showed the presence of streptococci similar to the cultures obtained from the bull and the ealf. ; There are two principal outstanding features in this case which serve as a motive for the presentation of this case report. First, an infection which can be traced back to the sire and from him to an infected herd where he originated, and later progressing in the form of an unbroken chain to a future generation, demonstrating the part played by the male in the transmission of genital infections and the ultimate chaotic results following in the dam and offspring. Secondly, that tubal diseases can be successfully controlled when uni- lateral, although such surgical intervention is very difficult due to the general inaccessibility of the genitalia in cows through laparotomy. OLEANDER POISONING IN A BEAR' sy W. J. RATIGAN New Orleans Zoological Park, New Orleans, La. SUBJECT—Black bear (Ursus luteolus), male, three years of age. ; The animal was observed to be ill in the early afternoon on April 14. My office was notified but I was unable to get to the Park until nearly evening. The bear was in considerable distress. The symptoms pre- sented indicated a severe gastro-enteritis. The animal had vomited several times before I reached its quarters, and while I was in attendance it changed positions frequently and gave, as bears do, every evidence of marked abdominal pain, 7. e., lying upon the back with the limbs extended, attempting to procure relief by grasping with the paws anything within reach, ete., ete. It is known down here that the bark and leaves of the oleander are toxic to animals. I couldn’t account for this animal’s con- 1 Presented at the fifty-eighth annual meeting of the American Veterinary Medical Association, Denver, Colo, September 5-9, 1921. | CursicaL AND CAse REporTSs U7 dition in any other way than to lay the blame upon its in- gestion of some of the leaves of this tree. The bark seemed un- disturbed. The tree was a dozen feet from the cage. The fol- lowing day one of the employees of the Park said he had seen a colored nurse, to amuse a child, pluck some leaves and place them in reach of one of the bears. The animal showed slight improvement until the following noon when it became worse and died that evening. Upon autopsy the stomach was found to be empty, its wall was considerably thickened and the mucous membrane was a slate or purple color. These changes extended into the duo- denum, where several blood clots were found, indicating the previous hemorrhagic inflammation. The following may be found in Law’s “‘Principles and Prac- tice,’’ vol. 2: DOGBANE FAMILY In Apocynacee must be named the Neurium Oleander (a common house plant in the North and a garden or wild plant in the South and West). Thevetia and Apocynum Andresemifolium (spreading dog- bane) are most deadly acrid poisons to all kinds of stock. In my experience a horse cropping a few leaves of an oleander died in a few hours with violent gastro-enteritis. The dogbane is less poison- ous when dried in hay. ; The Pasteur Institute at Paris produced during the period of the war about eight million doses of serum of various kinds, according to an interesting article reviewing the work of the Institute during the war, by Dr. A. Calmette, assistant direc- tor, in the Revue d’Hygiene. The American Army and the American Red Cross received 800,000 doses. At the time of the German offensive in Mareh and April, 1918, the Institute was able to furnish 20,000 vials of antitetanie serum per day. Sigma Xi, the honorary scientific fraternity, accepted Dr. Leonard W. Goss of the Veterinary Department, Ohio State Uni- versity, as an initiate at its recent meeting. Membership is based upon accomplishment and promise in the field of science. Dr. P. W. Loob, who has been engaged in Federal hog cholera work in Kansas for over a year, has recently been transferred to Iowa on the same elass of activities. ABSTRACTS VACCINATION AGAINST STRANGLES. René Van Saceghem. Ann. Méd. Vét., vol. 66 (1921), p. 100. After referring to the seriousness of the malignant epizootic form of strangles affecting horses at army remount depots, and after reviewing briefly the work of other investigators, Van Saceghem describes a method of vaccination which has been tried in a preliminary way with good results in the Belgian Army and which is being introduced on a large seale in that army. The vaccine is made of a two-days’ culture of the strepto- coccus of strangles on bouillon, heated to 56° C. for half an hour. The streptococcus employed is a culture isolated from pus from affected horses at the remount depot for which the vaccine is intended. Before being used the vaccine should be tested by making cultures to make sure that it contains no living organ- isms. The vaccination consists of three subcutaneous injections, the first of 5 ec. and the others 10 ¢.¢. each, eight days apart. The first injection always gives a greater local reaction than succeeding ones. The thermic reaction rarely exceeds one de- eree (Centigrade). Old horses show a much milder local and thermic reaction than young horses. This may be accounted for by a natural immunity of the older horses. Horses already affected with strangles, especially those convalescing, show no local reaction. The horses vaccinated by this method have not contracted the disease in the midst of heavy infection. The vaccine not only possesses preventive immunizing powers, but is also curative. After injecting subeutaneously repeated doses of 10 ¢.c. into horses gravely affected with strangles a mani- fest therapeutic action has been observed. In all cases the vae- cine influenced very favorably the course of the disease. PARATYPHOID OF THE Honry Bee. UL. Bahr. Skandinavisk Veterinar-Tidsskrift, vol. 9 (1919), pp. 25-60. In the digestive tract of bees affected with a severe intestinal diarrhea the author found a type of bacteria which he classified as belonging to the paratyphoid group of bacilli and to which he gave the name ‘‘ Bacillus typhosus alvei.’’ The disease mani- fested itself in producing in these animals slight paralytic 98 ABSTRACTS ) symptoms and diarrhea, which was exceedingly infectious. In a few days a considerable number otf bees were affected, the mortality being about 50 per cent. The organism, with few ex- ceptions, could be recovered in pure culture from the intestinal tract of infected bees, and it is said in some instances from the blood. The organism is characterized as rod-shaped and has a certain biological and morphological similarity to the ty- phoid bacillus, but it is not identical with that occurring in man. The disease can easily be transmitted to healthy bees by feeding them with pure cultures of the bacillus. In concluding his investigations, the author lays particular stress upon meas- ures for the eradication of the disease, particularly in controlling the purchase and sale of hives and queen bees. de bs O7uEARY: SEPTICEMIC INFECTION IN LAMBS CAUSED BY THE BACILLUS OF Swine Erysirpenas. M. Christiansen. Maanedsskr. Dyr- loeeger, 31 (1919), No. 12, pp. 141-254. Abs. in Exp. Sta. Record, vol..44 (1921), p. 583. This is a report of post-mortem examination made of a lamb 38 hours old in a flock in which there had been a very high mortality among the lambs, revealing hemorrhagic enteritis, great enlargement of the mesenteric glands, degenerative changes in other organs and small hemorrhages under the endocardium and epicardium. Small bacilli present in pure culture were found to represent the swine erysipelas bacillus. It is stated that no case of swine erysipelas in pigs had occurred at the farm during the time of the outbreak among the lambs. CoMPLEMENT FIXATION IN Bovine TuBercuLosis. C. Hruska and Wo Ptennmeer. Ann.’ Inst. Pasteur, vol. $5 (1921), p. 96. Abs. in Amer. Rev. Tubere., vol. 5 (1921), p. 98. The antigen of Besredka fixes alexin in the presence of serum of tuberculous cattle. In the sera df 304 known tuberculous eases, 84.5 per cent positive reactions were recorded. Of 90 sera where tuberculosis was not demonstrable at necropsy, 2.2 per cent reacted positively. Where tuberculosis was not ad- vanced (glandular only) the percentage of positive reactions was 60 per cent. Where the involvement included lungs, pleura, peritoneum and some of the abdominal viscera, the reaction 100 ABSTRACTS was 85 to 95 per cent positive, and when the disease was gener- alized all sera reacted positively. The method should be an im- portant diagnostic aid in bovine tuberculosis. EXCRETION OF TUBERCLE BACILLI IN Bm. A. C. Marchisotti. Semana Méd., vol. 28 (1921), p. 87. Abs. in Amer. Rev. Tubere., vol. 5 (1921), p. 98. Marchisotti found the liver often affected in tuberculous cattle. Healthy milk can thus become readily contaminated by droppings from tuberculous cattle, or the apparently healthy cow herself may be voiding tubercle bacilli by way of the stools. His research was done on cattle slaughtered for the market and thus supposedly healthy, the tuberculous lesions being a ne- cropsy surprise. Human urine and stools are liable to trans- mit the tubercle bacilli as readily as sputum. Tuberculosis of the liver should be classed as open tuberculosis on this account. Tubereulin tests do not reveal the involvement of the liver in particular. TUBERCULOSIS IN SMALL Ropents. Drs ‘ a a Pe 4 + Shaper ‘ ey | teenth plctoae Ate A tae Mie a Prat he Wn ae IcTERO-HEMOGLOBINURIA IN CATTLE 163 connection between this disease and the presence of excessive amounts of what might be called dead water and relatively high temperature. The nature of the pasture itself does not appear to be a material factor, cases being almost equally frequent on natural wild hay meadows and alfalfa pastures. The treatment of these cases can not be said to be on anything approaching a satisfactory basis at this time. In the absence of definite knowledge as to a bacterial cause, nothing which can be properly classed as a specific antiserum has as yet been used. Anti-hemorrhagic-septicemia serum has been used exten- sively, but we now know this material to have been nonspecific, and it will be considered merely as a serum in summing up this phase of the question. As a matter of routine practice, it is found that the intra- venous administration of horse serum in doses of 200 mils, preferably repeated several times at intervals of 8 to 10 hours, supplemented by the administration of fairly active but non- irritant purgatives, such as large doses of ground flaxseed boiled up in a liberal amount of water to which has been added a pound or less of sodium sulphate, is of benefit. Stimulants can apparently also be administered to some cases advantageously. Treatment such as outlined when started fairly early in the course of the disease appears to bring about a marked reduction in mortality, provided the ease is not of an unusually severe and acute or fulminative type. Cases of this sort and those in which treatment is started rather late in the attack appear to be but little influenced by therapeutic measures of any kind. Disregarding the extensive use of hemorrhagic septicemia _ bacterins and vaccines, which have proven valueless for reasons which are now quite obvious, this disease being in no way asso- ciated with infection by B. bovisepticum, very little has as yet been done along the lines of direct control by the use of biol- ogics. : Recently when indications pointed more and more strongly toward B. welchii as an etiological factor, work was undertaken along the lines of specific immunization, using an aggressin pre- pared by inoculating young cattle with this organism in the Same manner as is blackleg aggressin. We have to date treated approximately 1,200 animals with this aggressin in the districts where this disease usually causes the heaviest loss. Part of the 164 Epwarp Rrecorps AND LyMAN R. VAWTER material so used was prepared and supplied to us for experi- mental use by Dr. Wood, and the balance was prepared in our own laboratories. As this portion of the work was undertaken only during the present season, it is too early to make any definite statement as to the results obtained, but present indica- tions are that the use of this aggressin has afforded at least some protection, the percentage of cases occurring among the treated animals being appreciably less than among the untreated con- trols, an adequate number of which were left in each herd. Dr. Wood has also used this material to some extent in California, with what result we do not know. The more general measures of control which seem clearly in- dicated by our observations on the occurrence of this disease are unfortunately impracticable on account of the great expense involved. ‘To drain the lands properly where excessive water is now present either as a result of natural conditions or unju- dicious irrigation in the past would require in most cases engi- neering work of considerable magnitude. What it has been possible to do along this line on some ranches in the way of keeping cattle out of pastures where excessive water was pres- ent, and the practice of more careful irrigation so that dead water was not allowed to accumulate, has apparently resulted in a material lessening of the losses, and such procedures are cer- tainly indicated in the light of our present knowledge. It is of course obvious that if some thoroughly effective means of specific immunization could be devised it would‘afford by far the cheapest and most practical means of controlling this disease, placing it on a par with anthrax and other diseases which are now so well held in check in this way. Unfortunately the use of the aggressin referred to above does not give any present promise of being feasible on a large scale, even if it should prove effective in preventing the disease, owing to the fact that the very small percentage of successful inoculations of cattle with B. welchu for its production would make the cost almost pro- hibitive. This may, of course, be overcome by the devising of some more satisfactory technique for its preparation. The suc- cess of this aggressin would, of course, in any event, depend en- tirely on B. welchu being the actual causative factor in this disease. SURGICAL TECHNIQUE—SUTURING! By H. E. KincMAn Professor of Veterinary Surgery, State Agricultural College of Colorado, Fort Collins, Colo. IN presenting this paper, it is to be understood that no at- tempt has been made to cover completely the subject under dis- cussion. The writer is anxious to present the cases in mind in such a manner as to interest his fellow surgeons, leaving out all details that might be essential to a group-.of students or others less able to grasp the context than those for whom this paper is intended. It is presumed that we are past the basic steps and are interested only in solid facts. In this paper I hope to present and illustrate a phase of technique that has been very useful to me and I am sure can be to others. Suturing enters as an important factor in nearly every opera- tion, and frequently occupies as much time as incision, dissec- tion and hemostasis. In Cesarian section, for example, one’s time is principally taken up in suturing. Many other opera- tions demand an equal amount of attention to uniting the wound. Next to cleanliness, the operator is most concerned in thoroughness or carefulness, but following these in importance comes speed. A patient can withstand a severe operation, pro- vided the animal is not made to endure it for a long period of time and given large amounts of anesthetics or restrained in a cramped position. The time element enters in as a matter for careful consideration in conserving the strength and resistance of the patient and in obviating surgical shock. Speed in suturing goes a long way toward reducing the length of time that the patient must remain under restraint or anesthesia. With the idea in view of improving our technique in suturing, I wish to present several methods of tying knots. It is to be remembered that the hands are frequently covered with blood and that securing the ends of the suturing material is liable to consume more time than it should. The needle having been passed through both margins of the wound, it is held with the needle holders in the right hand. 1Presented at the fifty-eighth annual meeting of the American Veterinary Medical Association, Denver, Colo., September 5-9, 1921. 165 166 H. E. KinGMan The free end is picked up with the thumb and finger of the left hand and the opposite suture grasped with the second and third fingers. The sutures are then crossed until the free end may be drawn under by the second finger; releasing the hold with the thumb and first finger makes the first part of the knot. The free end is again grasped by the thumb and first finger, the second and third fingers are laid with the palms up, while the needle end is crossed over again and the second finger draws the other end under, completing the knot. Forceps may be used instead of fingers. The following method is preferred by many operators. We call it the two-forceps method. After passing the suture through both margins of the wound, the suture is wrapped around the end of thumb, forceps held in the left hand; the free end of the suture is then grasped with the forceps and drawn through the loop. The same thing is repeated in the opposite direction, completing the knot. The four-foreeps method is less practicable than the first two described, but is very useful when skilled assistance is available. The assistant grasps the needle end of the suture three inches above the wound with the forceps in his right hand; he then ties the first part of the knot the same as in the second forceps method. The operator then completes the second part of the knot by the same process. I wish to describe in detail the application of three sutures that have proved to be especially useful. The first, of these was the so-called interrupted mattress suture. Its advantages lie largely in the fact that it brings into apposition raw surfaces, turning the external surface away from the margin of the wound. To illustrate one instance in which the mattress suture is par- ticularly adapted, I wish to discuss the treatment of split ears. The split ear is of common occurrence and wherever barbed wire is found. However, this is not the only offender. One was brought to our attention in which a Jersey bull split his ear for a length of four or five inches on a nail, giving him a very unsightly ear, especially if he was to be used for show purposes. It is seldom advisable to attempt suturing an accidentally split ear until the edges have completely heated. The operator may then have a clean field upon which to work. The technique consists in preparing the field in the usual manner; the entire ear is serubbed with Dakin’s solution, and the area lathered with tincture of green soap. The hair should SuRGICAL TECHNIQUE—SUTURING 167 be shaved from both sides of the ear for at least a space of half an inch in width. Anesthesia consists in chloral enough to quiet the animal. Some beneficial results have been obtained from the use of apothesine over the posterior and internal auricular nerves. A sterile sheet with a hole in the center is placed over the head and the ear drawn up through the hole. All instru- ments should be sterile, as well as the operator’s hands. An incision is made on the edge of the healed surface, exposing two flaps of skin and the yellow fibrous concha. The flaps are loosened: to the extent of one-fourth inch and the protruding concha removed the entire length of the incision. The opera- tion is repeated on the opposite side. The cutaneous surfaces are then united up by means of mattress sutures of linen, one- half inch apart. By this method the fresh surfaces are main- tained in apposition. The inside and outside of the ear are sutured separately. The united surfaces are then covered with a solution made of gum mastic in benzol. At the end of three days the suture should be examind for signs of infection. Then if moisture is present or if there are other signs of pus, the infected suture should be removed and the wound bathed in alcohol or 1 per cent of tincture of iodin. Under ordinary conditions the wound unites by first intention. Occasionally a few of the sutures must be removed or treated with aleohol, but if given the proper attention small infected areas need cause no alarm. In fact, eases have been operated upon and given no further attention after the first day and made complete recovery, although a few of the sutures became infected. T should like to eall attention in detail to two other methods of suturing. The first of these is a continuous peritoneal and subeutaneous suture, used especially in small animals where the peritoneum and abdominal walls are to be closed. The thumb forceps lifts the peritoneum on the right side away from the viscera, and the needle is passed through the muscle and peritoneum. The peritoneum of the left side is then lifted and the needle passed through the peritoneum and muscle from beneath. The suture is then grasped with the fingers or forceps, drawing the peritoneal surfaces together and lifting them so that the needle may pass through both at the same time. This is repeated until the peritoneal wound is closed by a simple continuous suture. The skin is then grasped and held, while a 168 H. E. KingMan subcutaneous suture is placed in it. This suture is then ear- ried continuously from one side to the other until the skin wound is closed. The two ends of the suture are united by a surgeon’s knot. If No. 1 gut is used, the knot should be small enough to be completely hidden. 3 The second is the mattress suture as used in closing the wound and hernial ring in the treatment of ventral hernia. An elliptical incision is made over the fundus of the sae and the skin carefully removed, exposing the serous sae. This separa- tion of cutaneous and serous coats is continued until the hernial ring is reached. The tunie and fascias forming the ring are firm and may be easily recognized by the sense of touch. One frequently encounters a thick layer of fat lying close to the hernial ring, which impedes the progress of dissection. Hemor- rhage should be slight and is easily controlled by means of sponge and foreeps. One should avoid sponging as much as possible, since it is a common source of infection. Repeated wiping of the tissues lowers their resistance and inhibits repair. The next step consists of preparing the flaps of fascia for suturing. One begins by splitting the margin of the ring as close to the parietal peritoneum as possible, taking great care not to invade the peritoneal cavity. The flaps should be one- half to three-fourths of an inch in width. A full curved needle threaded with No. 4 catgut is then passed down through the left flap at a point one-half inch from its margin, down through the margin of the opposite flap, then up through the margin of the same flap and up through the left flap one-fourth of an inch from the starting point. This constitutes a mattress suture. Repeat the sutures over the leneth of the hernial ring. The sutures should then be drawn tight. This will foree the right flap beneath the left, causing them to overlap one-half inch. The free margin of the right flap should then be sutured to the left surface by means of a continuous suture. The skin should be sutured with a mattress suture, bringing into apposition eon- siderable denuded surface. A place for drainage is left at the anterior commissure of the cutaneous wound. One may insert a strip of gauze to be removed at the end of 12 hours. The entire wound, except the place for drainage, is covered with gum mastie and gauze. The wound will unite by first intention if reasonable care has been observed to avoid the introduction of infection. The skin su- ~ SurGicaL TECHNIQUE—SUTURING 169 tures may be removed in about five days; the catgut sutures are permitted to take care of themselves. The advantages of this technie over some of the others com- monly used are that the peritoneal cavity is not invaded, and further, that the overlapping of the fascias insures a perma- nent union and obliteration of the hernia. Also there is no danger of injury to intestine or other viscera. If one should fail in the matter of asepsis, there is little to fear from peritoni- tis, since one has.only a wound of the skin and subcutaneous tissues to treat. In ease of infection, one or two skin sutures should be re- moved and the wound dressed with Dakin’s solution, or a 2 per cent aleoholic solution of iodin. HORSES AND MULES IN WAR In public recognition of the important services performed by horses and mules in the United States Army during the war, a large bronze tablet has been placed on the east wall of the State, War and Navy building, Washington, D. C. It depicts a field artillery piece drawn by four spirited horses, with their riders and two cannoneers on the field of battle, and makes a vivid picture of action. Underneath in raised bronze letters is the following descriptive inscription : ‘This tablet commemorates the services and sufferings of the 243,135 horses and mules employed by the American Expe- ditionary Forces overseas during the great world war which ter- minated November 11, 1918, and which resulted in the death of 68,682 of those animals. What they suffered is beyond words to describe. A fitting tribute to their important services has been given by the commander-in-chief of the American Expeditionary Forces, Gen. John J. Pershing, who has written: ‘The Army horses and mules proved of inestimable value in prosecuting the war to a-successful conclusion. They were found in all the theatres of preparation and operation doing their silent but faithful work without the faculty of hoping for any reward or compensation. ’ ‘*This tablet is erected by friends of the horse and mule in the United States, under the auspices of the American Red Star Animal Relief, a department of the American Humane 4 THE VETERINARIAN AND PUREBRED LIVESTOCK 197 agent. Here let me deviate long enough to say that our county agent is performing a very valuable service along these lines. He appreciates cooperation and advice and is ready at any time to make a trip to the farm of an interested client to urge him to purchase a registered sire or other purebred livestock. I believe that this demonstrates, in a way, that the county agent is an asset to any agricultural community, and that the prac- titioner has been a little hasty in always assuming that the county agent is his enemy. The county agent has come to stay; his services are of value, and he functions much better as an interested friend than as an antagonist. It has been our pleasure and privilege to assist at various farm bureau and livestock meetings in advocating the improve- ment of breeding methods, and I believe that at no time has the stockman been more eager and willing to accept proven methods than at present. This type of service is somewhat unusual for a practitioner, but it certainly is ethical and of value to all concerned. Along this line the veterinarian can perform a distinct service to his community by assisting in the organization of purebred clubs, especially calf and pig clubs for the boys and girls. En- thusiasm for purebred cows is rivaled only by that shown for purebred pigs by the members of our boys’ and girls’ clubs. We can already see that the boy or girl with one registered calf or pig today will be the purebred breeder and good client of tomorrow. They take a keen interest in the particular breed of which they own one individual, and naturally they have many questions regarding type, feeding, care and management which the practitioner can be easily in a position to answer. These boys and girls are starting with only one or two individuals and are increasing the number as knowledge of their problems are gained; therefore we expect them to become the future well- informed, scientific purebred owners and clients. With their questions they are willing to go to the practitioner if they feel that he is vitally interested in their future success and able to give them sound advice. Another method of stimulating the purebred business and eventually the practitioner’s business is the showing of worthy animals at the various fairs. There is no greater incentive toward the desire for better animals than competitive showing at these fairs. The pride that comes from the ownership of a 198 GEORGE H. Carr winning animal is infectious and spreads in a community. Most owners of one or two purebred animals hesitate to show them because they are not familiar with methods of conditioning for show purposes and rules of entry. Going from one farm to another, and knowing the desired types, the veterinarian can greatly increase the number of animals shown by constantly advocating better breeding methods and informing the client how best to fit his animals, and by selecting worthy livestock to be shown. This service does not make a direct daily return to the veterinarian, but it certainly builds a good foundation for satisfactory future business. We have, as a class, been expect- ing too great immediate returns and have just begun to realize that upon the welfare of the livestock owner depends the future of our profession. Too many of our purebred owners are committing the error of registering every purebred animal regardless of conforma- tion to type or suitability as a breeding individual. This is a poor business policy and one which in a short time will cost the purebred livestock breeder more than he ean possibly real- ize on the sale of such animals, for nothing so reacts upon a breeder as the sale of an unworthy individual. The veterinarian can do a great deal toward eliminating undesirable breeding animals. Castration of scrub purebreds should be just as closely practiced as the castration of grade animals, and the practitioner need not hesitate in recommending such measures. He will be doing the individual owner and the purebred industry a favor by seeing to it that there is only a survival of the fittest. These are some of the ways in which the veterinarian can be of greater service to his clients, especially to those who own registered stock. It is a source of great satisfaction to have a good percentage of clients who are owners of purebred animals. Veterinary service is much more promptly and _ intelligently used. On purebred patients we do not experience so great a dif- ficulty in obtaining some consideration and care as we do in grade animals. The owner of a fifty-dollar animal hesitates a long time before calling the veterinarian, while the man whose three-hundred-dollar registered animal is sick usually seeks the best veterinary service available, and at once. This has quite a tendeney toward making better veterinarians by eliminating those who do not keep abreast of the times and consequently can not give high-class service on valuable ani- Sebbeedlalt sam digbemea one ee a o THE VETERINARIAN AND PUREBRED LIVESTOCK 199 mals. The time of the ‘‘old farmer’’ has passed, and in his stead we have the agricultural business man who knows, appreciates and is willing to pay for good service. There is certainly a greater stimulus toward the making of better veterinarians by linking the interests of the practitioner with the interests of the owner of purebred livestock. The vet- erinary profession is a young profession and has progressed only so fast as the value of animals has increased. Therefore let us have a concerted effort on the part of the veterinarians to place more and better purebred livestock on our farms. DISCUSSION Dr. O. E. TROY (Raton, N. Mex.): I wish to second Dr. Carr’s sen- timent regarding cooperation with the county agent. My experience has not always been the best with the county agent. I think we should solicit their cooperation and cooperate with them, instead of working in the opposite method. Dr. KINSLEY: The Doctor should be complimented on presenting such a paper as this to a group of practitioners. The practitioner can not afford to overlook the advantages of the purebred animal. Those men that are in the communities in which the purebred animals predominate we find are the thrifty veterinarians of today. The veterinarian, the practitioner, is dependent upon the livestock industry. The livestock industry, however, is the basic principle in itself. Your function is to further the benefits of the industry, and you can do this better in a community in which the animals are purebred. They are much more valuable to the owner. They are much more serviceable to our com- munity, to our State, and to our country, than are the scrubs. Nearly all the veterinarians are advocating purebred animals, but I fear a great many of the practitioners are not making careful study of the advantages and the individuals of the breeds. I fear that is one of the weak points, particularly of our older practitioners. The younger men have had the privilege that the older men did not have in college of ob- taining courses in this subject. I should like to see a lively discussion on this topic. DR. JAFFRAY: I want to draw attention to one little point the Doc- tor brought out there, about the pedigree of an animal. I was called down here a little while ago by an attorney in Chicago who was sued for $10,000 for a valuable sire which was supposed to be one of the finest around that point, and he wanted to know where he could get judges as to the value of that animal. It appears he had a copy of his pedigree, and he claimed he paid $10,000 for him. I said, “Have they backed that up with anything?” He said, “What do you mean?” I said, “What has this sire done? What can he show?” He said, “That is a good idea.” They defeated the law suit with that one point. They couldn’t show that that sire had followed up with the ancestors. There was just one law suit of $10,000 decided because they couldn’t back up what the animal did. CHAIRMAN FERGUSON: There are a lot of good points in this paper. I am interested in that particular point that the Doctor just men- tioned. I think that it is just as important to eliminate purebred scrub as it is the grade and the scrub. There are plenty of pure- bred scrubs, and the purebred industry has been handicapped largely by dealers. The purebred man has let the dealer run his business to a great extent, and it has hurt him. The time has come right now when that part of it must be eliminated. DISCUSSION OF PAPER ON PARTURIENT PARESIS' Dr. C. C. STEWART (Colorado Springs): I didn’t get to hear the first part of this paper, but I noticed an article in THE JOURNAL some months ago, by one of the members, who seemed to consider that he had a very unusual case, it having occurred seven days after par- turition, and I suppose it has been the experience of most of the men that it might occur then or later. I have had particular cases occur four weeks, and one six weeks after parturition, and if there is any- body who hasn’t experienced that, it might be well to keep it in mind, because I found that it will occur that long after parturition. From the article in THE JOURNAL it seems as though that particular writer was very much surprised and doubted his diagnosis somewhat for a time because it occurred seven days after calving. I don’t know what the general experience has been, but I had a case several weeks ago, in a Jersey cow, four weeks to the day after calving, and it was a typical case in every way and responded to the treatment. Dr. G. H. HUTHMAN (Portland, Oregon): We find that camphor and oil is better than strychnin. We use 30 c.c. Dr. J. B. PATERSON: In the line of treatment that this gentleman has used, it seems as though he advised not earlier than four hours. In a number of cases that I have had there have been return attacks after six hours, and in one case especially a calf had been allowed, to suck early in the evening and the cow was decumbent. In two or three hours we had her on her feet, but some time in the night the calf got with the cow again, and the next morning she was down. During the day we got her up and in twelve hours she gave a gallon of milk. We had another case where the cow was milked regularly, and every few hours she was inflated. CHAIRMAN FERGUSON: That is a good point to bring out—that relieving of the udder after the treatment is applied in relapsed cases is good. That very frequently happens, and the way I handle them in practice is to repeat the gauze. We don’t ligate the teats. We take a little rubber band and tie it over the teat, with instructions to the owner to remove it a little later. As a common procedure we don’t ligate the teat, but do make a return call on valuable cows, and in those cases have the owner milk them out, and inflate the second time, and feed the milk to the calf if it is thought advisable to feed him. It isn’t a good plan to feed the calf too early, and it is better to remove the calf from the cow’s stall to another stall so he can’t interfere with her udder at all. That is a good point to bring out, because the cow might be reported as up, and the milking of this animal might ke recommended, and the owner might lose her. A man with experience wouldn’t recommend this, but a young prac- titioner might. He might forget to have the calf removed, and the calf might remove the milk in the night, and it might cause the loss of the cow. Dr. G. H. Carr (Brighton, Colorado): I have had during the last month two cases in which the patient was extremely susceptible to strychnin. It was strychnin poisoning and it was quite alarming. Is there anything that you would give in those cases to allay those symptoms? I have had one death in the last three years from strychnin poisoning. SECRETARY MULDOON: I think there were a few things which the 1This d’scussion followed the reading of. the paper on ‘“Parturient Paresis,” by A. A. Motley, Alpena, Mich., at the fifty-eighth annual meeting of the American Veterinary Medical Association, Denver, Colo., September 5-9, 1921. Dr. Motley’s paper appearel in THE JourNat for October, 1921, vol. 12 (n. s.), p. 63. 200 Petri ran acne: 3 DISCUSSION OF PAPER ON PARTURIENT PARESIS 201 essayist did not bring out; that is, the fact of the temperature. He says it may sometimes be 103. We should not forget that the tem- perature oftentimes in the beginning of these cases goes to 105 or 106. There is a sharp curve, and then it drops down to subnormal. Then we must not forget that the animal sometimes shows nervous symp- toms in the beginning of parturient paresis. We should keep those in mind. One or two doctors have brought out the matter of strychnin poisoning. Personally, I don’t use strychnin. I use cocaine, if I use anything at all, as a stimulant. I like cocaine for things of that nature, and I use cocaine in place of strychnin. Im cases of this kind I use 2 to 3 grains. Sometimes I use more than that if I think it is necessary. I like cocaine better than I do strychnin in a case of this kind. Camphorated oil I am not particularly fond of. You are likely to get abscess formation and other things, if you have a dirty syringe. The fact is, you are likely to get abscess formation with camphorated oil, no matter how sterile you feel the injection is. Dr. HUTHMAN: We have tried camphorated oil two years and a half, in every case of milk fever, and we have still the first case of abscess to find, and we disinfect with iodin and lysol, and so on. So far we have had no abscess formation, where we use it subcutaneously. We don’t use it intramuscularly. We find we get much better re- ‘sults, and it is perfectly safe. Even as little as a quarter grain of strychnin has had a bad effect on animals, producing toxic effects. In a high-grade Jersey cow we had toxic symptoms from strychnin. CHAIRMAN FERGUSON: That is very true. - I believe it is the ex- perience of all practitioners that the cow is very susceptible at that time to the action of strychnin. If it is used, a small dose is indi- cated, and I agree with you in that camphorated oil a good many times does not cause the formation of abscess, although, as Dr. Mul- doon has said, I have seen a lot of trouble from camphorated oil, for some reason or other; it may be the mixture or preparation or some- thing of that kind. We have been mixing our own and use cotton- seed oil and ether, and we have no trouble. We inject that intra- muscularly. We don’t take any pains in sterilizing the skin. We select some point of skin to go through that is clean. We take a little pledget of cotton and wipe it off with iodin a little while before making the injection, and, of course, a clean syringe is used. The syringe is not boiled every day if we are not using it for something that might contaminate it. Dr. HUTHMAN: It may be that many use just common camphor- ated oil, and that may be where they get abscess formation. I have noticed the difference in camphorated oil and camphor and oil. We have had abscess from straight camphorated oil. We get the 30 c.c. ampule, which costs something like 20 cents. We charge that to the owner of the animal. We make a minimum charge of so much for the trip and so much for medicine and a general charge for injection of camphor and oil, 50 cents. Dr. STEWART: I have found that camphor and oil is all right if it is warmed to the temperature of the body. I have had abscess where I used it cold. The oil will naturally retain the cold temperature longer than when we make the solution warm. I have used caffein, sodium, and so on, when the patient was very nervous. CHAIRMAN FERGUSON: This question of abscess formation after the injection of the agent into a cow is an important one. If we are dealing with high-class cows, we must keep away from abscess as far as possible. It is well to know how to handle camphorated oil, where it is indicated. There is a time when the camphorated oil you buy 202 DISCUSSION OF PAPER ON PARTURIENT PARESIS is made with neutral oil. Some claim that that isn’t so good. - I never used any myself. These are all excellent points. Dr. PATERSON: I have had a number of cases of parturient paresis where there was a light-headedness and the cows were weak on their feet and wobbly, and would fall around, and not seem to know any- thing. In those cases I was just wondering whether I was treating the right disease or symptoms. CHAIRMAN FERGUSON: I believe you were. That is one of the symptoms. You may be called on to see a cow.and she may be a little wobbly on her feet and nervous. If you get a treatment in, it will very often prevent her going down. Quite often it will keep her on her feet. Those cases that come six or eight days after partu- rition respond to the treatment. Anything you get right near par- turition time is right sure to be parturient paresis. Of course you may get toxemia then. Those cases that come some time afterwards are mammary toxemia or toxemia from other causes. In those cases of mammary toxemia you will get good results from the air treat- ment, and you will find in most of those cases that if it isn’t present, if there is not noticeable symptom of it present at the time, a day or so later you will find you have had an insignificant mastitis, but enough to cause toxemia. Dr. HUTHMAN: I want to ask a question that has been called to my attention, about the farmers’ own treatment of a case, pumping it up. I have gone on two cases recently where the whole body surface seemed to be inflated with air, and I was wondering if there was any way by the use of the bicycle pump that they tore the skin loose from the udder tissues. Where that took place, my treatment has been the use of camphorated oil and hot applications to the back, and continually massaging the body. In two out of three I got re- sults. One died with pneumonia. I think the bicycle pump gives too much force. Instead of inflating the milk tissue, it tears the skin loose and inflates the tissue all over the body. Dr. D. S. JAFFRAY, JR. (Chicago): Around Chicago we have the box-stall cow, the cow that doesn’t get any exercise. We use the treatment a good deal, and we have cases of toxemia just as often as parturient apoplexy. I don’t agree with the Doctor in massaging the cow where the air has gone through her body. I think it well to leave that alone. I have had cases where I was called in afterwards, where the air had gone all through the muscles of the back. I never touch that. I use hot applications to the udder. Once I found an infection after the air treatment where a bicycle pump had been used. They had used no precaution to sterilize the pump, and there was infection due to the dirt in the hose. I think there is one point that should be looked into. ; DR. GRIzzELL (Kansas): Before entering college I was acting as herdsman for a large dairy firm in the East, and when I first came there we had a number of cases of parturient paresis. We made it a rule there never to milk the cow thoroughly for a week after calving, and we diminished these cases materially. I wondered since whether that did good or not. I have advised a few men to do that and they have. By leaving a portion of the milk in for a week or ten days, it doesn’t seem to hurt the udder any and does hold the animal back from being attacked with this disease. I want to take exception to one thing in the paper of Dr. Motley, and that is, that I have treated two . cows three years successively, with the history that they were treated one year previous to that, making four years. I would like to know whether anybody has anything to say about leaving some milk in the - udder. Dr. HUTHMAN: My experience has been the same. We milk about half of the milk three times a day, instead of all the milk twice a day. —a be 7 DISCUSSION OF PAPER ON PARTURIENT PARESIS 203 In cases that I have had two years in succession I tried it the third year and had no success. We also give a good dose of physic imme- diately after calving, say twelve or fourteen hours after calving. CHAIRMAN FERGUSON: What do you do with a case that you find stretched on the side, almost all in, and bloated? You have to relieve the bloat right away or it will be a case of asphyxiation. Then there is another class of cases, the kind that come down with it before the act of parturition has taken place, or during parturition. There are two good points to bring out. Dr. STEWART: I have found those cases, and the first thing I do is to get the animal on the other side, and before I get it on the sternum I use a little spirits of ammonia on a piece of cotton and hold that in the nostrils. I let them inhale some of the air that is more or less charged with ammonia. It seems to relieve them quicker than any- thing in the way of hypedermics or anything I have tried. CHAIRMAN FERGUSON: What do you do with those cases that haven’t calved yet, coming during parturition or a little before? How do you handle those? A MEMBER: How do you handle them? CHAIRMAN FERGUSON: The way I handle them is this: I give them the treatment before I try to deliver the calf. I give them the air treatment immediately. I get them into the proper position and then go on and deliver the calf. I prefer doing that way rather than to try to deliver the calf first. You have a very inert uterus and air treat- ment seems to invigorate it, and by giving a treatment and possibly leaving them an hour or so, the parturition is made easier and much safer for the cow. ; Dr. H. E. KINGMAN (Colorado): Do you find the cervix dilated? CHAIRMAN FERGUSON: Yes; it is dilated, but inert. There is a lazy uterus. It doesn’t affect the uterus, because that is demonstrated many times in the retention of the placenta. The placenta is already in shape to come, but there isn’t any vigor, and involution doesn’t take place or doesn’t start, and the placenta just remains lightly at- tached. If the cow receives treatment, in a few hours it usually passes away itself. If you apply the treatment before assisting the cow to deliver the calf, you will find it invigorates them. If you go to work on the cow before applying the treatment, you will have trouble. As to those cases of bloat I might say a word. I am referring to the case that is bloated to such an extent that the animal is almost asphyxiated. Of course we all know about tapping, and tapping will relieve them. If the cow hasn’t gone too far, roll her up on her sternum, and she will belch up, but it isn’t safe. The nicest way to do is not to disturb the cow at all, but take the stiff stomach tube and guide it down over the epiglottis into the esophagus into the rumen, blow out the clog and let the gas off. A stiff tube will follow’ down into the rumen and get the gas that is disturbing the animal. It is nearly always dry gas, and it will relieve it in an instant. The bloat will be relieved. Then give the treatment. It is safe to leave the cow on her side until you treat the udder. Roll her on her sternum and you have a good, safe prospect for recovery. The object in having this paper was to bring out a discussion that would better the treatment, and guard against being a little bit careless in applying one of the best treatments that we have ever had for any disease, the oxygen or Schmidt air treatment. The men in the profession belittle that treatment to the extent that they are a little careless with their equipment. They have a dirty old bicycle pump along, or an outfit that is ridiculous for them to use, and they attempt to make an impression with that. If they will get some sort of equipment that is out of the ordinary, like a water bottle with two tubes in it, and a nice bulb and a nice clean needle, 204 DISCUSSION OF PAPER ON PARTURIENT PARESIS you can make an impression on the owner that way. His herds- man may be using a dirty old outfit. He wants you to do the work. If you do it in a way that will attract his attention, it will mean a great deal. As to the hypodermic medication of animals with this disease, of course we all use it, and it is almost always indicated, and we know why. We are going to continue to use it. The impression that your treatment leaves goes a long way toward keeping up this good treat- ment that this man Schmidt has given us. He is about the only practitioner that gave the profession something that is big. This is- big, when you stop to think of the number of valuable cattle that used to die in the earlier days with parturient paresis. You must appreciate that this is a wonderful treatment, and Schmidt is the man that is responsible for it. He started in with the iodid potassium solution first, and then worked up to the air method. If we guard it carefully we will have a fine treatment and we can keep it right in the profession. If we have our outfits in proper shape to make an impression, that is all that is necessary. Dr. Chauncey E. Sawyer, of Carlyle, Kans., has been appointed as instructor in pathology at the Veterinary Division of the Kansas State Agricultural College. The veterinary extension work of the university of Penn- sylvania has been placed under the general supervision of Dr. George A. Dick, Professor of Animal Industry in the Veterinary School. Dr. Dick will have charge of correspondence and other administrative work and the other members of the veterinary faculty will assist him in giving addresses at meetings and in the other instructional work. Dr. Dick will continue his regular classes in animal industry in the Veterinary School. Dr. B. L. Dawson and Dr. F. J. Lingo, of the Federal tuber- culosis eradication forces in Florida and Ohio, respectively, have been transferred to similar work in New Mexico. Dr. Clement E. Wilmot, formerly engaged in Government work for hog cholera control in Iowa, has been assigned to tuberculosis eradication in Missouri. Dr. Paul Vaughn has been placed in charge of the Federal tuberculosis eradication work in North Carolina with headquar-- ters at Raleigh. CLINICAL AND CASE REPORTS (Practitioners and others are invited to contribute to this depart- ment reports of unusual and interesting cases which may be helpful to others in the profession.) INDURATED UDDERS IN VIRGIN HEIFERS By E. M. NIgHBERT United States Veterinary Inspector, London, England IN facilitating exportation of purebred cattle from Great Britain into the United States, on a recent visit to Scotland my ~ attention was called to a disease affecting the udders of young heifers prior to breeding them. The condition was commonly referred to by breeders as ‘‘udder clap.’’ I was informed by breeders that treatment was unsuccessful and that affected ani- mals were consigned for slaughter. History—tThe disease was reported to be quite common, developing during dry and unusually warm summers, when cattle would bunch in the pastures and seek shade. It appears from the information gained that not more than one to four animals would be attacked during a season on the same farm. The disease always appeared in young unbred heifers or well- developed heifer calves. Also the disease developed so slowly that it often escaped notice until the animal began to lose flesh or failed to keep in good condition under the customary care that the herd was receiving. The disease was reported to have been seen in unbred ewes. Symptoms.—tThe first symptoms noticed were that one and sometimes two teats would be prominently enlarged and hard to the touch, with acute local inflammation of one and sometimes two quarters of the udder. The disease would slowly progress until the affected parts would become greatly enlarged, giving the appearance of a developed udder in advanced pregnancy in an adult cow. The affected parts would be extremely hard to the touch, generally painless on palpation, except when abscess formation appeared superficially. The general appearance of the animal would show slight unthriftiness, appetite remaining good but development retarded. These are the symptoms de- seribed by the owner. I asked if I might be shown a case, and was promptly taken 205 206 CLINICAL AND CASE REPORTS to a local slaughtering establishment where two heifers between eighteen, months and two years of age had been sent for slaughter. One of the animals was affected with the udder trouble and [ had an opportunity to make a postmortem. Postmortem appearance——The carcass appeared normal and fairly fleshed. No enlargement or abnormal appearance of the body lymph glands. Abdominal and thoracie viscera normal, with no involvement of lymph glands. The udder was the only organ showing disease. One teat and quarter were involved, being greatly enlarged, reaching the dimensions of approxi- mately 6 by 14 inches. On palpation the teat and quarter showed extreme induration. In cutting through the quarter a hard fibrous mass was displayed, with pus pockets intermixed, filled with a greenish-hued fetid pus. The cut surface was smooth to the touch, and the parts affected displayed a progres- sive and prolonged interstitial inflammation. Differentiation.—The macroscopic pathological appearance showed no calcified deposits nor enlarged lymph glands as are seen in tuberculosis. The pus was thin, greenished hued and fetid, which would exclude actinomycosis. There was no appear- ance of local injury or point of infection. Therefore the con- dition indicated chronie garget commonly seen in aged dairy cows at the time of slaughter. In my experience in practice, in the field and in postmortem work in connection with United States meat inspection, this con- dition in young heifers was not observed in the United States. BREED AND SEX SUSCEPTIBILITY OF CALVES TO BLACKLEG By J. W. Lump Sioux City, Iowa THE QUESTION has often been raised as to what breed and sex of calves is the most susceptible to blackleg infection. Some contend that the beef breeds are more susceptible than the dairy or dual-purpose breeds. Others hold that the reverse is true. The data regarding sex and breed susceptibility appearing to be so exceedingly meager, it was thought that a short compilation of that kind might be of interest to the profession. —— CLINICAL AND CASE REPORTS 207 The calves from which these data were compiled were bought on the open market in mixed lots without reference to breed or sex, keeping, however, within a certain weight and age limit. They were inoculated with a standard dose of pure virus for the routine production of blackleg aggressin. Each calf received practically the same dose of virus, administered in the same way. This virus had been previously standardized so that varia- tions in the virulence of the virus would be practically negligible. Almost every lot of calves presented for inoculation at any one time was made up of both males and females, as well as being of a mixture of breeds, all of which tends toward a uniformity of results in eliminating unequal variations. The calves were all grade calves. Any that did not show a distinct breed type were put in the no-type column. Table 1.—Classification by Breed 1918 1919 1920 | Per | Total | Total cent Breed Inocu- | Im- Inocu- | Im- Inocu- Im- Inocu- | Im- | Im- lated mune| lated | mune| lated | mune| lated | mune! mune =i Aberdeen-Angus. 36 6 | -32 5 29 8 97 19 19 Hereford. 75. ..! 50 Sealine Ome ui |peelsn 172 30 352 55 15 olstemsen ones. . 77 9 123 21 84 22 284 52 18 ETSOY teste ate os 30 4 42 | 9 19 2 91 15 16 Red Polled...... 18 2 71 | 11 66 8 155 21 13 Shorthorn...... 354 54 491 | 92 488 103 | 1,333 249 18 INowLypen ako o 71 13 112 24 ; 119 18 | 302 55 18 It will be seen from Table 1 that the breed susceptibility varies but shghtly, the limits of the immunes in all the breeds falling between thirteen and nineteen per cent. Three of the seven classes each showed 18 per cent of immunes. Figuring the percentages by years for the Shorthorns we get 15 per cent, 18 per cent and 21 per cent. This leads to the conclusion that the great number of Shorthorns used gives us a more reliable per- centage of immunity than we get from the small lots used of Aberdeen-Angus (19 per cent) and Red Polled (13 per cent). Had there been as many animals inoculated of these breeds as of Shorthorns the per cent of immunes might have been brought to approximately the same level. The conelusion, then, would be that the breed has very little if anything to do with the susceptibility of the individual animal. 208 CLINICAL AND CASE REPORTS Table 2.—Classification by Sex 1918 | 1919 1920 Per iret a |) Potala hotalimcent Class { Inocu- | Im- Im- | Inocu Im- Inocu- Im- | Inocu- Im- lated | mune | mune | lated | mune} lated | mune| lated | mune Heifers-.. a5. 265 53 416 79 425 100 | 1,106 232 PAI Bidisenss skh ck 180 23 239 28 207 18 626 69 11 Steers’ ce ee, | 236 20 367 54 310 55 913 129 14 In figuring the yearly percentages of immunes of the three types inoculated, in Table 2, we find that the heifers in all three years, using a large number of animals, showed a persistently high per cent of immunity as compared with either the bulls or the steers. There is not a great difference between the num- ber of heifers and of steers inoculated. While the num- ber of bulls is only a little more than half that of the heifers, it would seem as if enough animals were used to form a repre- sentative group. It will be seen that a total of the males gives an 8 per cent lower average immunity than for the females. The conclusion would be that heifers are, when inoculated, more highly resistant to blackleg than the males. TRAUMATIC PERICARDITIS By B. J. FINKELSTEIN Cherry Valley, N. Y. I REPORT the following case as being both interesting and somewhat unusual : Holstein cow, five years old. Saw her first on August 8. No history other than that she had dropped off in her milk, and refused to eat. Examination showed the following: Tempera- ture, 102.3; pulse, 118; respiration, 24. Cow was recumbent, but would get up without much urging. On auscultation over the heart region I could determine a splashing sound. Rumina- tion had not stopped, according to the owner. I could not de- tect any rumen contractions. On percussion over the region where reticulum lies cow showed marked pain. I diagnosed the case as traumatic gastritis and administered a saline purgative. Told the owner the cow would die and I should like to ‘‘post’’ her after she had gone to bovine heaven. 5 paemes ce ’ CLINICAL AND CASE REPORTS 209 [ heard, nothing of this case until August 24. Then the owner called on me and informed me that the cow was still alive but very weak. He agreed to butcher her so that I might perform a postmortem. When I saw her again I found on aeenanntien that the pulse was very weak and frequent (I could not count the number of beats per minute). The brisket and submaxillary space were both markedly edematous. However, the feces looked normal. Postmortem showed the following: Liver enlarged to twice its normal size, was friable, and the capsule peeled easily. Lungs both congested throughout. Heart was about five times normal size, and the muscular walls were taut against the pericardial sac. On opening the reticulum a thin, evil-smelling pus slowly oozed away. I followed the course of the abscess and found a piece of wire about three inches long on the pleural side of the diaphragm. Here the abscess was just about the diameter of the wire. The pericardial sac was punctured and there was a small hole in the right ventricle. On opening the right ventricle about four quarts of the same thin, evil-smelling pus was released. The endocardium was thickened and rough. The valves leading from the right auricle were hard and greatly enlarged. The right auricle, although somewhat enlarged, appeared quite normal. The left side of the heart showed no very marked changes other than that the muscle was somewhat thinner than usual. I have wondered since why this cow did not die of acute septicemia. The heart weighed 14144 pounds with its pus con- tents. DYEING DOGS IN PARIS Reports from Paris indicate that many women are having their pet dogs dyed a tint to match the gowns they wear. The blonde, henna, rust and brown shades are easily accom- plished as coats of both Chows and Pekes responded well to the usual peroxide and henna treatments, but some difficulty has been experienced in securing matching lavender dachshunds and purple Pomeranians. The fashion has been further complicated by disinclination to use German dyes on French dogs. ABSTRACTS MeASURES TAKEN IN SWITZERLAND AGAINST Foot-AND-MouTH DISEASE IN Recent Years. L. Panisset. Rev. Gén. Méd. Vét., Jan. 15, 1921, pp. 1-12. (Abs. in Bul. Inst. Pasteur, vol. 19, p. 536.) These measures are grouped under three main headings: General slaughter, sanitary measures, and treatment with blood of recovered animals. Slaughter is a good measure at the beginning of an epizootie, when there are but few centers of infection. It may also be useful at the end of an epizootic, in freeing the country of isolated centers. But when the disease is widespread this measure becomes useless. Sanitary measures, although well applied in Switzerland, have proved effcacious only in the first centers quarantined. When the centers become numerous all sanitary barriers are powerless to prevent the extension of the disease. It is rather difficult to determine the value of treatment with the blood of recovered animals. This treatment is not pre- ventive. It seems capable only of diminishing the gravity of the natural development of the disease. THE TONSILS AS A Port OF ENTRY FOR INFECTIONS. Julius Citrona. Deut. Tier. Woch., 1920, no. 27, p. 240. The physiological function of the tonsils has not been fully explained. Their functions may be excretory, as well as being the point of entry for infections and at the same time they may be apparently normal. In producing experimental paratyphus the normal lymphatic esophageal ring has been found to be the point of entry for infections per os. It is therefore probable that in this manner natural infection with typhoid and paratyphoid takes place. Infection of the tonsils is not proof of their being a port of entry for infecting material. This is only probable, as tonsillar affections precede general infections. Tonsillar re- lapses lead to chronic tonsillar diseases. Injuries to the ton- sils give rise to fresh attacks and only tonsillectomy favors 210 ABSTRACTS 211 recovery. The connection between relapsing chronic super- ficial tonsillitis, polyarthritis rheumatica, glomerular nephritis, acute endocarditis, pleuritis, and cryptogenic sepsis is simply the result of tonsillar infections. J. P. O’LEary. THE PREPARATION OF POLAR STAINS IN VARIOUS BACTERIA. E. Epstein. Arch. fur Hygiene, vol. 90 (1921), pp. 136-154. Polar staining is not characteristic of any particular species of bacteria. It may be induced quite generally in bacilli, and hence has no differential diagnostic value. The usual heat fixation with the flame injures or destroys polar staining. Alcohol fixation conserves it. Polar staining following alcohol fixation is not an artifact; a true morphological picture is obtained. Organisms must be cultivated in fluid media to show the polar staining; when grown on solid media only few species show it. Polar staining seems to be related to the vital processes connected with growth and fission of the organisms, and is caused by the intake of water. W. N. Bera. CONCERNING OTITIS EXTERNA PARASITICA OF THE Cat. D. Bruderlein. Deut. Tier. Woch., 1920, no. 27, p. 231. The treatment of a malady produced by the acarus, Der- matophagus auricularis felis, is accomplished by a thorough cleansing of the external auditory canal. The agent best suited for this purpose is liquid paraffin. As specific remedies the following are recommended: 3 per cent creolin, balsam of Peru, styrax liniment, or 5 per cent oil of caraway. These remedies are applied at 2 to 3 day intervals. They possess ex- cellent parasiticidal properties, are non-irritating, are readily miscible with oils or fluid fats, and in «addition are easily pre- served. The animal’s bed should be treated with 2 per cent formalin solution or creolin water. The author has found that when fresh mites are placed in liquid paraffin they die on the fifth day. From this he concludes that the general opinion which exists broadcast that the mite is destroyed in a rela- tively short time as a result of suffocation is not substantiated. J. P. O'LEARY. 212 ABSTRACTS Etiotogy oF AcuTE GANGRENOUS INFECTIONS OF ANIMALS. Hilda Hempl Heller. Jour. Infect. Diseases, vol. 27 (1920), pp. 385-451. In this very interesting article the author reviews the three great groups of anaerobic invading microorganisms found during the world war in human wound infections. Of these three groups the writer has placed the Vibrion septique as being first in incidence in animal infections. Blackleg is placed second in incidence in the anaerobic animal infections. The writer has examined thirteen specimens of bovine muscle tissue all of which yielded the blackleg organism. In three instances the Vibrion septique organism was also isolated, as well as the blackleg organism. In no instance was a pure Vibrion septique infection found. The author has also studied fifteen cultures of anaerobic mi- croorganisms recovered from cattle by different imdividuals. Ten of these cultures were found to be Vibrion septique strains, and five were found to be blackleg strains. Eleven dried sheep muscle tissue samples and one culture recovered from sheep constitute the investigations made by the author on the anaerobic infections in sheep. The one specimen originating in the United States proved to be blackleg, while the other ten received from Europe and labeled ‘‘Bradsot’’ were found to be Vibrion septique infections. In so far as the theme of this paper is concerned, the sheep investigations if presented in the abstract would be misleading, since practically all of the instances given were cases of Euro- pean bradsot infections, and do not represent the anaerobic infections found in sheep in this country. Throughout this article the author contends that spontaneous Vibrion septique infections in cattle and sheep are common, pre- sumably because in the examination of thirteen samples of bovine muscle tissue, three Vibrion septique strains were isolated in addition to the blackleg organism. The author is of the belief that in these three instances the animals in question died as a result of a mixed blackleg-Vibrion septique infection. In spite of the fact that in no instance was a pure Vibrion septique in- fection encountered and on the strength of the three cases of alleged mixed infection, the writer draws the rather remarkable conclusion that the veterinarian does not know blackleg disease me e+e ad ABSTRACTS 213 when he sees it, that the majority of the veterinary profession has misunderstood the etiology of the anaerobic invasions of cattle, or must deliberately include, when making a diagnosis of blackleg, all infections caused by the Vibrion septique group of organisms, as well as the blackleg organisms. In view of the seriously faulty technique employed by the author in insolating the anaerobic microorganisms from the tissue specimens examined, together with the lack of evidence to support the conclusions drawn, it is more than probable that the writer’s judgment concerning the anaerobic infections in ani- mals is'as badly warped as is the expressed opinion that ‘‘the attitude of the veterinary profession has been that we already possess all the necessary knowledge concerning blackleg.’’ Most certainly the judgment of one so inexperienced in the anaerobic infections of animals can not be taken seriously or in any degree to alter the mature judgment of those investigators who for years have studied these diseases in the field as well as in the laboratory. W. S. GocHENOUR. AN INVESTIGATION INTO THE PURITY OF AMERICAN STRAINS OF Bacituus Boruuinus. George F. Reddish. Jour. Infect. Diseases, vol. 29 (1921), pp. 120-1381. The author calls attention to’ the fact that descriptions of Bacillus botulinus by various investigators of this country differ markedly in certain respects from those of European investiga- tors. In his study of this organism, the author employed nine- teen different strains of the organism obtained from various laboratories in different sections of the United States. In every instance but one, impurities in the culture were observed in the stock strains, the contaminant having all the characteristics of Bacillus sporogenes. When Bacillus sporogenes and Bacillus botulinus are present in the same material, they are so closely associated that it becomes a difficult task to separate them, the former, being the less fastidious of the two, is favored by the method of isolation generally used by American workers. The author’s results explain clearly the reasons for the sharp dif- ferences noted in the descriptions of the organism given by the European and American investigators. Furthermore, the use of impure cultures in the making of antiserum is unsatisfactory 214 ABSTRACTS and may account for the absence of uniformity im the results of treatment of botulism victims. Thus, the use of antiserums in the prophylaxis of botulism has given good results experimen- tally in some instances, but it has not as yet been pronounced a success in actual practice. The toxicity of the strains of Bacillus botulinus which are contaminated with B. sporogenes will vary with the age of the culture and with the proportion in which the contaminant is present. The author summarizes his work as follows: Nontoxic strains were isolated from 18 out of 19 so-called ‘*strains’’ of B. botulinus examined. These nontoxic isolations were proved to be B. sporogenes. Isolations of B. sporogenes were made from stock ‘“‘strains’’ of B. botulinus by 4 different methods, that is, by the use of anaerobic plates, simple dilution, aerobic plates with Staphyloc- cus aureus, and by heating in order to kill vegetative forms after the spores of B. sporogenes had been formed. ‘The ‘‘strains’’ of B. botulinus examined resemble morpho- logically and in their action on meat mediums and milk, pure cultures of B. sporogenes, except that the action of B. sporogenes is more rapid, and in some ecases carried nearer to completion. CALCIUM AND TUBERCULOSIS IN Raspits. R. Massini. Schweiz. Med. Wehnschr., vol. 161 (1921), p. 238. Abs. in ‘Amer. Rev. Tubere., vol. 5 (1921), p..102. Massini injected young rabbits with calcium chloride after they had been inoculated with tuberculosis, and found that they lived a little longer than the controls. A SouRING OF BEEF CAUSED BY BacinLUS MEGATHERIUM. Hubert Bunyea. Jour. Agr. Research, vol. 21 (1921), pp. 689-698. The author has made an interesting study of a condition known as ‘‘sour beef’’ which occasions no little economic loss, especially to firms which have no facilities for the storage of meat to prevent this alteration. As a result of his studies, the following conclusions were drawn: The phenomenon known as the souring of beef is a bacterial one. ABSTRACTS 215 The organism responsible for the souring of beef is Bacillus megatherium. Bacillus megatherium will sour beef under a wide range of temperature, but not in the absence of oxygen. In the souring of beef by Bacillus megatherium propionic acid is produced. Bacillus megatherium is nonpathogenic for experimental ani- mals (rabbits and guinea pigs) and does not produce an appre- ciable amount of toxin when propagated upon raw beef. DIsTeMPER IN THE Pic. J.P. M’Gowan. Scot. Jour. Agr., 1920, vol. 3, No. 4, p. 407. This paper discusses an epidemic, among pigs, of what the author terms distemper, the disease which is usually observed in dogs and eats. The origin of the infection was traced to newly purchased sows which were suffering from mild ‘‘eolds,’’ thought to have resulted from the train journey. However, in about a fortnight after the first of these sows had farrowed the disease broke out among their pigs in a very severe form. Later the infection spread quite generally to all the young pigs in the piggery. The symptoms in the older animals were usually mild, con- sisting of lack of condition, a soft, husky cough, and sometimes a definite paresis of the hind quarters. Recovery was the rule. Iu young pigs the symptoms were much more marked and the mortality was high. There was sneezing, eyes reddened, mat- tery, and lids gummed together; discharge from the nose, and a husky cough. In some cases there developed red papules on the skin, which. later became tipped with scabs. In late stages of the disease there was slight diarrhea and sometimes paralysis, par- ticularly of the hind quarters. The sick animals had a ‘‘doggy”’ smell similar to that present in distemper of dogs. Postmortem examinations on a number of pigs showed quite constantly pneumonie lesions of greater or less extent. No pathological alterations were found in the abdominal organs. Bacteriological examination of the trachea and lungs yielded cultures of the distemper organism (Bacillus bronchisepticus) in all cases. The heart blood was sterile. The disease was effectively controlled by enforcing strict sani- tary measures. These included frequent spraying of walls of 216 REVIEW sties, passages of pig houses, ete., with hot lime wash; confining pigs to their own sties; removing sows about to farrow to a clean, lime-washed house; avoiding transferring infection on utensils, feed, and person of caretaker; keeping dogs and ecats— out of pigs’ houses; killing off hopelessly ill pigs. In addition to these, a vaccine prepared from various strains of the distemper organism was used. It was recommended that pigs should be injected about four days after their birth. L. T. GILTneEr. THE Use or A Two Per CENT WATERY SUSPENSION OF ANIMAL CuHarcoaL (CArBo MrpicrnALiIs MERCK) IN LumMBAGO (Azo- TuRIA). D. Detlefsen. Berlin Tierarztl. Wehnschr., vol. 37 (1921), no. 27, p. 315. A ease of azoturia was treated by injecting 200 cc. of a 2% watery suspension of animal charcoal intravenously. In the course of a half hour following the injection the symptoms were aggravated, but after three or four hours the condition of the patient improved, and on the second day of the attack the horse had practically recovered. In septic diseases and in catarrhal fever of the upper air pas- sages, the same treatment gave good results. In all instances it has proved harmless and the cost is insignificant. Doses of 100 to 200 ¢.c. are recommended. L. T. GILTNER. REVIEW Lasoratory MANuauL IN GENERAL Micropiotocy. Prepared by the Laboratory of Bacteriology and Hygiene, Michigan Agri- cultural College, Ward Giltner, Head of the Department. Published by John Wiley and Sons, Inc., New York. Price $3.50. This is a second edition of the Laboratory Manual in Gen- eral Microbiology issued in 1915. A comparison with the first edition shows that the author and his coworkers have added greatly to the excellence of their previous work. The steady advance of knowledge in microbiology and laboratory technic requires that standard books on these subjects be kept up to date. That the author is alive to this fact is shown by the ex- REVIEW 217 tensive revision and the mass of new information which the work contains. That a book of 472 pages, including 42 pages devoted to the index, and a large, valuable list of references, can encompass so great a fund of practicable information is a tribute to the concise, clear manner of expression of the author. The exercises are enlivened by frequent, appropriate explanations for the steps taken or agents used. The purpose of the manual is to supply the student with detailed information which will make him more independent in the course of his laboratory work. The subject matter is divided into three major parts and an appendix. Part one is primarily for the purpose of giving a working knowledge of laboratory methods used in the study of microorganisms, in- cluding molds, yeasts and bacteria. Part two consists of exercises demonstrating the various physiological activities of microorganisms. Part three deals with applied microbiology and includes exercises on the microbiology of air, water, sewage, soil, the dairy, plants, and exercises on animal diseases and immunity. All told, the three parts contain 129 well- chosen exercises accompanied by a profusion of helpful illus- trations. Detailed instructions are given for the various biological tests, and for the preparation of important diagnostic agents, toxins, antitoxins, bacterins and vaccines. A 100 page appendix gives numerous formulae, tables and a wide variety of information of great value to the laboratory worker. A book of this character not alone fills the purpose for which it was primarily intended in a most satisfactory manner, but finds a ready place in all bacteriological labora- tories because of its value as a reference text on laboratory technic. Years of teaching and laboratory work in their several specialties have given the author and his associates an oppor- tunity to study the requirements of students in general mi- erobiology, and the book represents an accumulation of their observations and material obtained from the best sources of microbiological knowledge. Bs AS Gy ARMY VETERINARY SERVICE EXAMINATION FOR APPOINTMENT An examination for appointment as second lieutenant, Vet- erinary Corps, Regular Army, will be held November 14, 1921, throughout the continental limits of the United States, to fill sixteen (16) vacancies, under the following provision of law: ‘‘ Appointments in the Veterinary Corps shall be made in the grade of second lieutenant from reserve veterinary officers be- tween the ages of twenty-one and thirty.’’ (Sec. 24e, Act of June 4, 1920.) Applications for this examination will be made on the blank form for application for a commission in the Regular Army (Form 88, A. G. O.). This blank form may be obtained at any military post or station or from Corps Area Commanders, or from the Adjutant General of the Army, Washington, D. C. Appleations, after completion, will be forwarded to the Com- manding General of the Corps Area in which the applicant re- sides or to the Commanding Officer of the nearest military post or station of the United States Army. In ease the post or sta- tion commander receives an application he will immediately for- ward it to the Corps Area Commander. It will be noted that the law quoted in the first paragraph re- quires that applicants for appointment be selected from reserve veterinary officers. Consequently, if an applicant is not a mem- ber of the Veterinary Officers’ Reserve Corps, he will, before examination, make application for membership direct to the Adjutant General of the Army, Washington, D. C., stating that the appointment in the Officers’ Reserve Corps is desired to make him eligible to take the Regular Army examination. In addition to being a member of the Officers’ Reserve Corps, an applicant must be between the ages of twenty-one and thirty years at the probable time of appointment, which will be about two months after the examination. He must also have a satis- factory general education, must be a graduate of an accept- able veterinary college, legally authorized to confer the degree of Doctor of Veterinary Medicine, or its equivalent, and which requires students to have covered satisfactorily a four years’ high school course, or its academic equivalent, as a minimum 218 ARMY VETERINARY SERVICE 219 entrance requirement, and which maintains this course of in- struction covering a period of four years of not less than seven months in each year. The applicant must also have had, sub- sequent to graduation, at least one year’s experience in the practice of veterinary medicine, or its equivalent in hospital work, or as an instructor in an approved veterinary college, or as an employee of the Bureau of Animal Industry of the United States Department of Agriculture, actively engaged in vet- erinary professional work. AN ARMY VETERINARY PICNIC The Army veterinary officers attending the School of Meat and Food Inspection in Chicago gave a very delightful picnic in Jackson Park on the afternoon of September 22. Those present were Colonel and Mrs. Steel, Major and Mrs. Mill, Major and Mrs. Jewell, Captain and Mrs. Whitney, Captain and Mrs. Houston, Captain Eakins, Lieut. and Mrs. Juzek, Lieut. and Mrs. Lovell, Lieut. and Mrs. Curley, and Lieut. and Mrs. Herbott. Lieut. Wolf, a lone bachelor, managed to forget his loneliness with two young ladies. Dr. and Mrs. A. H. Baker, Dr. and Mrs. D. M. Campbell, Dr. and Mrs. N. S. Mayo, Miss Dorothy Mayo and Miss Marguerite Jones were also guests. This year a careful watch was kept on Dr. Campbell and the base of supplhes was not raided. There was surely some ‘‘chow.’’? A bushel of fried milk-fed chicken ‘‘an’ every- pioiage. 7? After a successful attack on the eatables Mrs. Baker made a motion that. all the civilian veterinarians present join the Army, where they would have such fine eats and nothing to do. This started hostilities immediately, but owing to the excellent organization of the veterinary corps the civilians were willing to refer the matter to the Disarmament Conven- tion in Washington. After the treaty of peace had been concluded a delightful time was spent conversing until dark, when a swarm of Hun mosquitoes from ‘‘no man’s land’’ made a concerted at- tack. As silk hose is not an efficient protection the forces, both military and civil, were withdrawn to previously prepared positions. N. S. Mayo. AMERICAN VETERINARY MEDICAL ASSOCIATION Proceedings of Fifty-Eighth Annual Meeting, Denver, Colo., September 5 to 9, 1921 MONDAY MORNING, SEPTEMBER 5, 1921 The first session of the fifty-eighth annual meeting of the American Veterinary Medical Association convened at 9:45 a.m. in the City Auditorium, Denver, Colorado, President David S. White presiding. The invocation was delivered by Rey. James E. Davis, pastor of the Central Christian Church of Denver. PRESIDENT WnuitE: Ladies and gentlemen, there are three rea- sons why I am very happy to introduce to you the gentleman who will deliver to you the address of welcome. The first and most important reason is that he is a near-Ohioan. He confesses to having been born as near Ohio as he could have under the cir- cumstances, namely, the State of Michigan. (Applause. ) Secondly, he has been out in this great western country nearly half a century. A large part of this time he has been engaged in the cattle industry. Thirdly, he happens to be the Mayor of Denver. I take great pleasure in introducing to you, therefore, the Honorable Dewey C. Bailey, Mayor of Denver. (Applause.) ADDRESS OF WELCOME Mayor Batmrey: Mr. Chairman, Ladies and Gentlemen, Mem- bers of the American Veterinary Medical Association: As your chairman has said, for a great many years I was in the livestock business in Colorado. My ranches were out about sixty miles southeast of Denver. A great many years ago I bought and brought a great many southern cattle into this northern coun- try, before they shipped them on the railroads or thought they could. We drove them or trailed them across into this country and Wyoming. Texas fever was distributed over this northern country by these southern cattle. There was not a large loss by death, but it continued along the trail where these cattle were driven until veterinarians and men well experienced in that disease and in the cattle business made up their minds that there was no doubt but what that disease was brought into this country by southern cattle. So I have made up my mind since being requested to make an address of weleome that there were a great many things that I knew of that shoyved that the veteri- narians of the United States were an exceedingly useful body of doctors of this country. While in the ranch business I also raised a great many horses. I started in the livestock business in a very small way and grew 220 PROCEEDINGS OF A. V. M. A. ripe up with it. I can remember an old span of horses that I had started with that were as loyal and faithful and trustful and hard-working a team as a man ever saw. I loved them. I loved a great many of the horses that became personal pets of our family as the years rolled by, but especially this team that never failed me, helped me make a living, and honestly (that was forty-odd years ago), I dream of that old team occasionally yet. They made such an impression on me that I never forget them and never will. Of course, in handling a great many of these horses, raised in this country, I had use many times for the veterinarians. Since I have been Mayor, before our fire department was motorized, we had all the way from 100 to 165 head of horses. I came in contact with a number of veterinarians in that way, and “especially Dr. Dunleavy, who looked after our horses; and I want to say that the help and kindness shown to these dumb animals, the most loved, the most intelligent and hardy of any animal grown in the world, makes me see what a great necessity there has always been for veterinary surgeons. I am not disposed to make a long talk. I know you are here for business. The good people of Denver, through their Mayor, extend to you greetings and a most hearty welcome to Denver. We hope this convention will be successful in its work and duties. We hope you will like our city, as I am sure you will. Where else could you see such a morning as we have here this morning—cool, the air bracing? Denver is a city of 270,000 people. Times are good here notwithstanding the reports that you hear from other sections of the country. Denver gives more free amusement to its citi- zens than any other city of its size that I have ever heard of. There will have been held in this city by the first day of Jan- uary 90 conventions. People like to come here, and we like to have them. I wish again to extend to you a most hearty welcome to the City of Denver. (Applause.) RESPONSE TO ADDRESS OF WELCOME PRESIDENT WHITE: Dr. H. E. Bemis, of Ames, Iowa, will respond to the Mayor. (Applause. ) Dr. Bemis: Mr. President, Mayor Bailey, Ladies and Gentle- men: I learned to take orders from Colonel White during the war, and no matter how difficult the task was that he ‘asked me to do, all I could do in those days was to salute, say ‘‘ Yes, sir,’’ and proceed to do the best I could. I remember when we were in France when he got tired of office duties he would start for a trip to the front, knowing full well when he left that the wait- ing list in the horse hospitals of. France was longer than the waiting list in the hotels of Denver at the present day. How- ever, in spite of that fact, about the second day after his 222, PROCEEDINGS oF A. V. M. A. departure I would receive a telephone message to prepare to receive about three thousand animals within the next twenty- four hours. Of course, all I could do was say ‘‘ Yes, sir,’’ hang up the receiver and proceed to do the best I could. But I did have one other loophole in those days, and that was to practice the good old army method of passing the buck, and simply get busy on the telephone and distribute these animals to a number of the already overcrowded hospitals. So in this instance, when I received word a few days ago that the President expected to have me make the response to the address of welcome, I simply said to myself, ‘‘ Well, the Colonel has got ’em again! When, oh when will this cruel war be over?’ But this time I was not able to pass the buck. To think of being welcomed to a city like Denver and a State like Colorado, with apologies to our friend Briggs, ‘‘Ain’t it a grand and glorious feelin’ ’’ simply to be here? Why, sir, you couldn’t keep us out! We have been prepared to come to this city and this State for the last five years, by the wonderful word pictures of our good friend Dr. Glover. You may think we didn’t want to come to Denver because we delayed so long after his first invitation, but I assure you that was not the reason at all. We were simply so inspired by his oratory that we wanted to wait for the grand finale which he gave us last year in Columbus, in spite of the effort of as good a State as Iowa, when he landed the convention, and we, are glad to be here and be welcomed here today. Colorado has the opportunity and has learned the happy lesson of combining work and play. This State, as we all know, is called ‘‘the Playground of America,’’ ‘‘the Switzerland of America,’’ and yet Europe can boast of no such pageant as the Rocky Mountains show us every day from the streets of Denver or from the trips which we are to enjoy. The land of magnifi- cent heights, of awe-inspiring depths, of roaring waterfalls and laughing brooks and ozone-laden air and rejuvenating waters— we realize and appreciate them all. Surely the Creator has ex- pressed himself in superlatives in the State of Colorado, and without any stretch of the imagination we can believe with Shakespeare that there are ‘‘tongues in trees, books in the run- ning brooks, sermons in stones, and good in everything.’”’ But there is another reason, perhaps a more special reason, why this particular body should be glad to come to Denver and to Colorado, it seems to me, and that is on account of the name and fame of this State in her industries. We have long known of her resources in iron and coal, gold and silver, and the precious metals, but we are more particularly proud of her accomplishments in agriculture and in the good ally of agricul- ture, veterinary medicine. Colorado has many special problems to solve in veterinary PROCEEDINGS oF A. V. M. A. 223 medicine. Some years ago she came to Iowa to get someone to head the work, and for a good many years the rest of America has watched these men and their progress and has been proud of their accomplishments. It is a gratification to know that Dean Glover and his corps of workers, and Dr. Lamb and his workers, and the practitioners of the State of Colorado, though few in number, yet large in efficiency and earnestness and en- thusiasm for the work, have been able to make their impression upon such people as Mayor Bailey, and I am sure upon the State as a whole. So it seems to me that we should be particularly proud and happy to come to Colorado to do these men honor, and it is our hope that the presence of this body in this city ail give them new inspiration for the work which lies ahead. I am sure, Mr. Mayor, it is the pleasure of all present to thank you for your gracious welcome to the City of Denver and to the State of Colorado. (Applause.) PRESIDEN'T’S ADDRESS PRESIDENT WHITE: The next item on the program is the Pres- ident’s address. (President White delivered his address. It was published in THE JOURNAL for October, 1921, page 9.) PRESIDENT WHITE: The next: item on the program is the pre- sentation and adoption of the minutes. Secretary Mayo: I hereby present the official report of the last meeting at Columbus as the report of that meeting. (On motion of Dr. Munce, seconded by Dr. Kelley of Albany, N. Y., the minutes were adopted as the officral report of the preceding meeting. ) Adjournment. MONDAY AFTERNOON GENERAL SESSION The meeting convened at 1:30 p. m., President White pre- siding. Report oF Executive Boarp PRESIDENT WHITE: The first item is the report of the Execu- tive Board, which will be presented by the Secretary. (Secretary Mayo read the list of applications for member- ship. ) (On motion of Dr. Kinsley, seconded by Dr. Connaway, it was voted to suspend the rules and instruct the Secretary to cast the ballot of the Association for the election of the persons whose names had been read.) PRESIDENT WHITE: Is there any further report from the Executive Board? . SEcRETARY Mayo: Ihave here the applications of Dr. W. F. 294 PROCEEDINGS oF A. V. M. A. Klee of Lima, Peru, a graduate of Royal Veterinary College of Copenhagen, Denmark; Dr. Carlos Lloveras of Buenos Aires, Argentina; Dr. L. Santa Maria, Pedros Negras, Mexico, graduate of the National Veterinary College of Mexico; Dr. H. P. Shepard, Killeen, Texas, graduate of the Southwestern Veteri- nary College, 1916; Dr. W. R. Smith, North Brookfield, Mass., graduate of Harvard Veterinary College, 1898; Dr. E. H. Sterling, Uruguay, graduate of the National Veterinary School of Uruguay. These gentlemen are graduates of veterinary schools that are not recognized by the Association. Four of them, I think, are graduates of national veterinary schools of other countries. One is a graduate of Harvard. That veterinary school is no longer in existence; in fact, it went out of existence before this Asso- ciation had an accredited list of veterinary colleges, although I think graduates of that school have always been admitted. The Southwestern Veterinary College is no longer in existence. When a veterinary school has gone out of existence the Con- stitution and By-Laws provide that the graduates may be elected to membership under suspension of the rules five years after the college has suspended. The Executive Board recommends that the rules be suspended and these men whose names I have read be elected to membership. (On motion of Dr. H. P. Hoskins, seconded by Dr. Connaway, the recommendations of the Executive Committee were approved and the persons whose names were read were admitted to mem- bership in the Association. ) SECRETARY Mayo: It is recommended by the Executive Board that the Constitution and By-Laws be amended as follows: Section 7, Article G, by adding: ‘‘Excepting members resid- ing in countries other than the United States and Canada, the said ballot shall be returned in ninety days after the date of issue.”’ This section deals with the election of members from the various representative districts. We have members in the Philippines, and it is impracticable to get a ballot to the Philip- pines and get it back within the sixty days provided by the Constitution and By-Laws. Another district aside from the one including the Philippines is South America, so that there are really two districts that will be affected by this. The recom- mendation is simply to change the Constitution and By-Laws to give ninety days’ time, to get a ballot to and from these foreign countries. Another recommendation for a change in the Constitution and By-Laws is that Article 11 of the By-Laws be changed by adding: ‘‘Excepting the Editor, Secretary, Business Manager and Treasurer, who shall assume their duties within thirty days following their election.’’ PROCEEDINGS OF A. V. M. A. 225 At present the Constitution and By-Laws provide that the officers shall assume their duties immediately upon election. The annual meetings of the Association come just about at the closing of the fiscal year, or the beginning of the fiscal year, which is on the 1st of September. At this time the dues are being collected from the members and there is an immense amount of work connected with these offices. It makes it im- practicable to change them immediately at that time. They really have to have a little time to get things entered and straightened out. It has been customary for several years to ete the old Secretary hold over until the end of the month and get things in shape for his successor. That is to provide for that. Another recommendation by the Executive Board is to change Article 5, Section 5, to read: ‘‘That the Treasurer shall give an acceptable bond to the Executive Board.’’ At present the Constitution and By-Laws provide that the Treasurer shall give an acceptable bond in the sum of $10,000. On the recommendation of the Treasurer last year, the Board increased that bond to $25,000, the amount of money, approxi- mately, in the treasury of the Association. You will note that this recommendation does not specify any amount that the Treasurer shall give bond for. That is left entirely to the Executive Board. If they should have $50,000 they can then demand a $50,000 bond. PRESIDENT WHITE: You have heard the further report of the Executive Board. This may be received now and will lay on the table until the next annual meeting. (On motion of Dr. Kinsley, seconded by Dr. Hoskins, the recommendation of the Executive Board was accepted and the recommendations for amendment were laid on the table. ) SecrETARY Mayo: The question of the ethics of certain forms of advertising has been brought up by members of the Associa- tion. This refers particularly to advertismg by moving pic- tures, and the Executive Board recommends that advertising by moving pictures, except the veterinarian’s name and address, should be considered unethical. PRESIDENT WuHitTe: You have heard the report in regard to advertising through the medium of the moving picture. This would constitute an amendment to Article 19 of the Code of Ethics. SEcRETARY Mayo: I hardly think it would be considered an amendment, but rather an interpretation of the Code of Ethics. PRESIDENT WuiTe: Moving pictures do not seem to be spe- cifically included. If you desire to take it as an interpretation, it would not be an amendment. Secretary Mayo: I think it would be a great help. Some firms supply veterinarians with slides and films, dealing, we will 2°26 PROCEEDINGS oF A. V. M. A. say, with hog cholera, in which the veterinarian’s name appears in connection with certain brands of serum. The matter was brought to the Seeretary’s attention by the Resident Secretary from this State. The Secretary doesn’t like to pass the buck, but sometimes I like to have some backing in the matter. At other times matters have come to my attention and I have taken them up with the manufacturers. These slides and films will go out to veterinarians in country districts, and sometimes they don’t consider whether it is a violation of the Code of Ethics or not. One of the manufacturers, in reply to my request that he discontinue it because I didn’t think it was ethical, said that he wanted to do what was ethical, but he would lke to have this Association’s opinion on it. That is why it was brought to the Executive Board and to the Association for an expression of opinion. PRESIDENT WuHite: The Chair will recognize expression of opinion in regard to this action of the Executive Board concern- ing the use of the moving picture as a medium of advertising. Dr. E1cHHORN: I move that recommendation of the Executive Board be adopted. Dr. JENSEN: I would like to hear that recommendation again. I presume this attack is directed at the firm I represent. The Government has been using posters to encourage and stimulate the farmers to vaccinate hogs, and in order to help out the idea promulgated primarily by the Government we took it up and offered to get our friends these slides. Considerable criticism arose, and when we heard of it, we advertised it and put it in our little paper. Dr. Mayo, I think, had written a letter saying that it was not considered ethical. That is as far as we are connected with it. When we took the matter up we thought we were rendering a real service to the country and to the hog raisers. Dr. Georce Hiron (Canada): Section 5 of Article 19 of the Code of Ethics distinctly states that in advertising the veteri- nary surgeon shall confine himself to his business address. It also states that advertising specific plans of treatment, medi- cines, advertising through the medium of posters, illustrated stationery, or newspapers, will not be countenanced by the Association. I think the first sentence in that section defines exactly how far we can go in advertising matters. I, therefore, second the motion that the Executive Board’s recommendation be accepted. SECRETARY Mayo: For Dr. Jensen’s information I may say that some firms supply posters, for instance. Dr. JENSEN: That leaves me out. SECRETARY Mayo: Some firms supply little posters with pranc- ing hogs and grinning hogs, with the veterinarian’s name. With one firm particularly, I took it up. I wrote them as nice a letter PROCEEDINGS OF A. V. M. A. rey | as, I could, telling them I didn’t think it was ethical and that I thought they were leading some young veterinarians astray, so to speak. They said they didn’t want to do that and so on, but they rather questioned my judgment in the matter and said they wished the American Veterinary Medical Association would take it up, and that they would stop if the Association said they should. Dr. ConNAway: It seems to me that there is a rather deeper ad more vital matter concerned in this. The moving picture is Pein a fine means of education of the laity on many of these things on which they should be educated. It seems to me that we are opening the way for carrying to the farmers much mis- information about the ways and means of controlling animal diseases. I see in the room just back of you certain methods of treating contagious abortion, for instance, which to my mind, if those doctrines are spread through moving pictures and in other ways, will carry a menace in a very forceful way. I think we ought to be very careful as to how we back up the advertising business to the laity by commercial concerns and through their agents, the practitioner who may be using their products. This is a more important thing than the simple question of whether some individual practitioner is ethical or not. This is a matter I would like to see thrashed out. Dr. L. L. Guynn (Monte Vista, Colo.): I am Resident State Secretary for this State, and, as Dr. Mayo said, he passed the buck to me. The reason [ brought this proposition up was that I had been asked whether that would be ethical. The reference was made by firms supplying serum, and the advertising of the slide would be confined strictly to that firm, but the veteri- narian’s name would appear at the bottom of the slide as the agent supplying that particular serum. I didn’t think tke thing was ethical advertising, and hence referred to the Secretary. That is the reason I wrote the letter and probably the reason why so much was stirred up. I didn’t think that any but au- thorized agents for that particular firm should have their names connected with the particular firm supplying the serum. (It was voted, on motion of Dr. Eichhorn, seconded by Dr. Hilton, that the report of the Executive Board regarding mov- ing picture advertising be adopted.) ; SECRETARY Mayo: The question of appointing a special com- mittee to help formulate a Federal Narcotic Law was referred to the Executive Board, and they recommend that the President be authorized to appoint a committee, not to exceed three, to confer with the representatives of other associations on the re- vision of the Federal Narcotic Law. (It was voted, on motion of Dr. Eichhorn, seconded by Dr. Adams, that the recommendation of the Executive Board with reference to the Federal Narcotic Law be accepted.) 228 PROCEEDINGS OF A. V. M. A. Secretary Mayo: The question of publishing a directory of members with the Constitution and By-Laws was considered for the coming year, and in view of the financial condition of the Association it was recommended that the names of the new members be published in the JourNAt of the Association, also the names of the new committees, the resignations, deaths, etc., and that no directory be printed this year. (It was voted, on motion of Dr. Kinsley, seconded by Dr. Hoskins, that the recommendation be accepted. ) SECRETARY Mayo: This is a report of the Executive Board meeting in Chicago last November of which most of you have been informed through THE JourNAL. At the suggestion of Treasurer Jacob, it was moved that the bond of the Treasurer of the American Veterinary Medical Association be increased to $25,000, which was done. SECRETARY’S REPORT PRESIDENT WHITE: That concludes the report of the Execu- tive Board. The next item is the report of the Secretary. (Secretary Mayo read his report, as follows:) The Association has now an active membership in good standing of 3,935, and 39 honorary members. During the past year 17 members have died, 12 resigned and 17 have been reinstated. Three hundred sixty-three have been dropped for nonpayment of dues. There are about 225 applications for membership this year. ‘The general economic depression that prevails has had a marked influence upon our membership, as it has on practically all associa- tions. One large and influential organization has reported a loss of 25 per cent of its membership during the past year. The number of members who have delayed sending in their dues is greater than ever before, in spite of special efforts to collect them, and the number of letters expressing financial stress has been much greater than usual. ; The interest in the Association and its work has been excellent. The expenses of the Secretary’s office, aside from the Secretary’s salary, may be classified as follows: Printing ‘and (stationery: ewer 40). cae ee $1,488.41 Clerical help cnt28 socieac ta Os. 668.27 POSUAGE nse cee carta te ee hy 378.36 Office ‘Supplies. en ee a eg Neg Sl, 71.27 “aneidentals’ 21228. 8 Ces S07 ene ea ae 11.92 Hreipht and hauling 24. be ee eee 64.57 Storage. charges’: 20 c.* 2 tek aie), nn Pa 21.00 Buttons for the meeting 18.00 Expenses of Columbus mectin go. ae. teen eee 150.30 The printing and stationery item includes stationery not only for the Secretary’s office but also for Resident Secretaries and various committees, and also for printing the directory of the members, com- mittees and officers of the Association, as well as the Constitution and By-Laws. Four thousand five hundred copies were printed, and a copy was sent to each member of the Association. A few copies were sold. Previously the directory with the Constitution and By-Laws had been published with the proceedings of the Association in an “Extra Proceedings Number” of the JOURNAL OF THE A. VY. M. A. PROCEEDINGS OF A. V. M. A. 229 On account of the ruling of the Postmaster General, it was not pos- sible to follow the plan previously in force. The cost of printing the directory was $560, and the postage for mailing was $80, a total of $640, which was charged to the General Association Fund. This ex- pense had been previously carried on the Journal Fund. Your attention is called to the fact that the influence of the A. V. M. A. is extending to other countries, and this year we have applica- tions for member’ship from Mexico, Uruguay and Argentina. Prac- ti all countries in the Americas are represented in our Associa- tion, and we have members in various parts of the world. If notices were inserted in some foreign journals calling attention to the advan- tages of being a member of this Association, it is quite probable that members could be obtained from other countries and the scope and the influence of our Association could be made world-wide. There is no veterinary association in the world that compares, either in size or influence, with the American Veterinary Medical Association. It is interesting to note that one of our members, Dr. G. A. Roberts, of Sao Paulo, Brazil, was the first to recognize an outbreak of rinder- pest in Brazil, the first time that this disease has ever been reported upon the American hemisphere. The term of the member of the Executive Board for the Fifth District expired. A postal card nomination and election was held in accordance with the Constitution and By-Laws. The following were nominated: Drs. C. E. Cotton, W. F. Crewe, C. P. Fitch, W. B. Spencer and C. H. Stange. After the votes had been received and before they had been counted, a number of requests came in from members to change their ballots to some other candidate. This prob- lem had never been presented before, and the matter was referred to the Executive Board. The Secretary was instructed by the Executive Board to count the first official ballot as it was sent in. Dr. C. E. Cotton, of Minnesota, was elected member of the Executive Board from the Fifth District. In preparing the Constitution and By-Laws for publication in the directory, some changes appeared to be desirable. The proposed changes were submitted to the Executive Board for their consideration and will be presented in the report of the Board. According to instructions of the Executive Board, the Secretary obtained from the former Librarian of the Board, Dr. Frost, of Ithaca, N. Y., the material that was in the Librarian’s possession. Dr. Frost asked the Secretary for directions as to shipping, and was advised that if the weight of material was less than 100 pounds to send it by express; if over 100 pounds, to send it by freight. The Secretary was somewhat surprised to receive two and a half tons of books by freight. Nearly all of this was made up of printed reports dating from the year 1906. Fortunately, the material was sold for nearly enough to pay the expense. The Secretary was instructed by the Executive Board to advertise in THE JOURNAL and to sell as many copies of the reports as he could for 25 cents each. This was done, and a few dollars’ worth were sold. The balance was ordered sold for old paper with the exception of a few sets that were selected and made as complete as possible in order to supply possible inquirers. So far as the Secretary knows, there is not a complete set of the Reports of the Association that belongs to the Association. If any member of the Association can supply reports earlier than 1890, the Secretary will be very glad to receive them and will see that they are preserved as a permanent record of the Association. Very few complaints have been received from members who do not receive their JOURNAL promptly. The few reports that have come in have been taken up promptly with the Editor of THE JouRNAL, who 230 PROCEEDINGS OF A. V. M. A. has given his hearty cooperation in getting the mailing list arranged and kept up to date. Most of the complaints received are from mem- bers who have changed their addresses and have failed to notify either the Editor or the Secretary. It is important that we, as individual members, should render all the assistance within our power to the Editor of THE JOURNAL in helping him make it more valuable in every way. During the past year your able President has attended a number of State and local association meetings as a representative of the A. V. M. A. This has helped very materially in bringing the A. V. M. A. into a more sympathetic and practical relationship with these associations, and this has been of mutual benefit. With the growth and development of the Association and its widen- ing field of usefulness there comes an increased expenditure of funds. Part of this is due to general high cost of living and part to the general tendency to expand. This is proper, provided the expenditure is warranted by the results to be obtained and the situation of our treasury. It is important that the Association as a whole in con- vention assembled and the individual members should carefully study the expenditures and proposed expenditures so that they may be fully informed how the funds of the Association are used. The matter of procuring a permanent home for the Association is already under consideration by the Executive Board and will probably be put into effect before many years. It is well that we should keep this in view and be prepared to meet the obligations which this will impose. At the last meeting of the Association in Columbus, Ohio, the dis- tribution of the Veterinary Relief Fund was placed in the hands of the President and the Secretary of the Association. During the year relief has been given to the wife of one of our honored members who died, leaving his immediate family and two old ladies, the doctor’s mother and his wife’s mother, with very little means of support. Upon the recommendation of the local veterinarians in that State, as well as the Chairman of the Executive Board, the sum of $500 was left available to the doctor’s widow. Five hundred dollars was contributed last year as an additional contribution to the Anglo-American-Franco-Belgian Veterinary Relief Association. This was acknowledged in a very grateful letter from Professor Vallée, who also sent a printed report showing in detail how the funds have been expended. The Association has a substantial fund for the relief of needy mem- bers and their families, and any member of the Association knowing of cases where the fund can be used judiciously and effectively are urgently requested to take the matter up immediately either with the Secretary or the President. The American Veterinary Medical Association has taken an active part in securing proper recognition for the Veterinary Corps in the United States Army. There are still some changes that should be made in order that the Corps may be more efficient, and I recommend that this matter be given consideration either by the Committee on Legislation or a special committee appointed for this purpose. In preparing the program for the annual meeting of the Associa- tion the different sectional presidents and secretaries are expected to prepare the programs for their respective sections. In some instances articles are secured for one section that properly belong in another section. The final rearrangement depends on the Secretary of the Association. It is recommended that the chairmen and secretaries of the different sections provide their programs sufficiently early so that any readjustment that may seem best can be made directly with the officers of the sections, rather than to leave the matter until the last PROCEEDINGS OF A. V. M. A. 231 moment before going to press. It is believed that this arrangement will prove more satisfactory. You will note that the program for this meeting is not as full as for several meetings previous. Formerly the meetings have been crowded, and it-was decided, after consultation with the Executive Board, that it woul better to give more time for discussion and for general business. It is hoped that this slight change will meet with the approval of all. It is important that the membership of the Association be increased. At present approximately one-fourth of the eligible veterinarians of the United States and Canada are members. While this is a higher percentage than that of the American Medical Association, we should have a much larger membership. At present the problem of getting new members depends largely upon the Resident Secretary. Some Resident Secretaries have done excellent work and some have done practically nothing, in spite of all the stimulating efforts that I could bring to bear upon them. In this connection I wish to thank the inspectors in charge as well as officials of local associations of the Bureau of Animal Industry for their splendid assistance in getting new members. We ought at least to double our membership. There are very few students graduating from veterinary colleges at present, and we must depend upon the practitioners in the field for increased membership. Doubling our membership would not only greatly in- crease our revenue but would extend the influence of the Association. I recommend that this matter be given your careful consideration and that a committee be appointed to carry the plans into effect. In con- nection with increasing the membership, such a committee could be of value in getting new subscribers for THE JOURNAL and stimulating an interest in it. I wish to express my appreciation for the cordial support and assist- ance given by the officers of the Association, the committees, the Executive Board, and individual members in carrying on the work of the Association. (Applause. ) PRESIDENT WHITE: You have heard this very excellent report of the Secretary. What is your pleasure in regard to it? (It was voted, on motion of Dr. Munece, seconded by Dr. Kinsley, the report of the Secretary be received and referred to the Executive Board.) TREASURER’S REPORT PRESIDENT WHITE: The next item of business is the report of the Treasurer. Dr. Jacop: The Treasurer’s report has been prepared and printed in pamphlet form. It has been distributed among the members, and it gives detailed information regarding the Asso- ciation’s financial affairs. Secretary Mayo: Mr. President, I have had an opportunity of seeing this report, and I think that Dr. Jacob can give a brief statement of the general financial condition of the Asso- ciation, and I think the members ought to have such a brief report. There are a good many that might not understand the report as given in this formal way, and I would like to have him present such a brief report. I know he has one. 232 PROCEEDINGS oF A. V. M. A. PRESIDENT Wuite: You have heard the suggestion of the Secretary that the Treasurer give us an abstract of his very ex- cellent report. Dr. JAcos: For the benefit of the Executive Committee, so that they might understand more readily the exact status of the financial affairs of the Association, I got up a little supplemental report. To a considerable extent it is embodied in the general report. Our total balance, cash and bonds on hand, at, the time that the books were closed for the fiscal year, was $27,341.26. Out of this, $20,000 (or about that amount) is invested in United States Government and Canadian bonds; that is, it has been the policy of the Association to purchase the bonds on an equal basis between the United States Government bonds and the Canadian bonds; consequently, $10,000 was put into each. We have been dividing the Association’s funds into three parts, the Association proper, the Journal Fund and the Relief Fund, and have tried as nearly as possible to keep these ac- counts absolutely separate so that we may know without any difficulty just how each part of the Association is operated. One of the most interesting things, probably the most im- portant thing, for the Association to give consideration to, is that our net profit, so to speak, our net gain for the past year, for all the activities of the Association, was $978.36. In other words, we have run practically even. This includes also the uncollected accounts, which amount to a little over $1,250. The interpre- tation of that is this: Had it not been for the interest we col- lected during the past year, our Association would have run behind. The condition was a little bit different during the year 1920, when the net gain was $3,329.78. So that you see we are losing ground a little bit. That is an important point and one not to lose sight of. So far as the Association proper is concerned, the sis: gain during the past year was only $47.31, practically even, and the little gain that we made was on the part of THE JOURNAL, where we made $912.37. That, in a few words, I believe covers the status of affairs of the Association. It might be of further interest, however, to know just how this money is handled. As I stated at the beginning, the $20,000 representing the face value of the bonds at maturity is, of course, held in bonds, and the interest is collected at regular intervals. It is necessary in order to meet the demands of the Association to keep a certain amount of money on check deposit, and it has been my policy to keep as small an amount of money on check deposit as it was possible to just squeeze through with, in order to be able to keep as much as possible on time deposit. The time deposits, of course, can be converted to the checking account at any time. Just at this time we have $7,000 on time deposit. PRoceEDINGS oF A. V. M. A. 233 Since the report was made out $3,000 worth of Canadian bonds, which were short-time bonds, matured; consequently I had those cashed, and we have on time deposit just at this time $7,000, and practically $3,000 covering the three funds for checking. No funds are paid by the Treasurer except on orders from - the President and the Secretary covering the Association Fund, the Editor and the President covering the Journal Fund, and the President and the Secretary covering the Relief Fund. PRESIDENT WuiTeE: I would like to ask the Treasurer if all of the money belonging to the Association is in the hands of the Treasurer, or is not some of it in the hands of special commit- tees? Are there any other funds to be accounted for? Dr. Jacop: So far as I know the only money that is not in the hands of the Treasurer is the money that has been main- tained by the Salmon Memorial Fund. Who is holding that money at the present time I don’t know. As far as I know, that is the only one that I don’t handle. Dr. J. R. Mowuer: Mr. President, I would like to say, in re- ply to your request regarding other funds, that the last check I sent to the Treasurer was on the 31st of July, in order that he could get it in this year’s report. Since that time we have collected over $500 in cash and $900 in bills receivable in the form of notes. So with this $1,400 added THr JouRNAL earn- ings would be a little better than the $912 indicated by the Treasurer. Dr. JAcos: That shows our best revenue is THE JOURNAL. SECRETARY Mayo: That is good in a way, since that report covers the matter thoroughly; but that doesn’t alter the fact that the Association ought to know that we are just about run- ning even at the present time, and that is what I wanted to bring out in this report. If the Journal Fund had published the Constitution and By-Laws last year, my funds would have been $600 better off. Another thing that you should, know is that at present all the funds turned over to the Treasurer by the Secretary are divided on a three-fifths basis; that is, 60 per cent of it goes to the Journal Fund. My understanding is that according to the Constitution and By-Laws only three-fifths of the dues ought to be turned over to the Journal Fund. You understand that con- siderable revenue is derived or sent in by the Seeretary aside from dues; for instance, this year there are 180 applications for membership. Each one pays $5 initiation fee. Three-fifths of that is turned over to the Journal Fund. I have collected a few other funds from various sources that are turned in, not dues at all. That is also divided on a three-fifths basis. The Journal Fund gets three-fifths of that, and two-fifths of that goes to the general Association Fund. I am not saying this in a eritical 234 PROCEEDINGS OF A. V. M. A. way. It is all Association funds. It is your fund and belongs to the Association, but I thought you ought to know that. PRESIDENT WHITE: Does any member wish to ask of the Treas- urer any question? Dr. Hoskins: The question was raised as to the Salmon Memorial Fund. I am not a member of that committee, but I can say that the funds are on deposit in the name of Salmon Memo- rial Fund in the Rittenhouse Trust Company. They are on time deposit and drawing interest. I believe there is about $4,000 net fund. My father was secretary-treasurer of the committee, and the account is in the name of the fund. SECRETARY Mayo: I think the Association ought to know, too, of a fund of $500 that was voted, I think, at the Philadelphia meeting and was in the hands of Dr. Thomas Smith of New Jersey. It was a fund designed to afford some immediate relief to members of the Association who were starting overseas and found themselves about strapped when they got to the Atlantic coast. The balance of this fund that wasn’t expended, I think four hundred thirty-eight dollars and some cents, was turned over to me by Dr. Smith, and I turned it in to the Treasurer. That, I would say, was not divided 60-40. Dr. Stance: If I am not out of order I would like to make a motion that we extend a vote of appreciation for the services of Dr. W. Horace Hoskins as the secretary and treasurer of the Salmon! Memorial Fund, and that the funds now be turned over to the Treasurer for his care. (The motion was seconded by Dr. J. G. Eagle, and carried. ) (It was voted, on motion of Dr. Munce, seconded by Dr. Hilton, that the report of the Treasurer be received and referred to the Auditing Committee. ) REPORTS OF COMMITTEES PRESIDENT WHITE: The next item of business is reports of committees. I will call for these committees in the order in which they are printed in the program. (The reports of the Sub-Committee on Journal, Committee on Intelligence and Education, Committee on Legislation, Com- mittee on Resolutions, Audit Committee and Committee on Necrology were called for, but these committees were not ready to report.) PRESENT WHITE: Is the Committee on History ready to re- port ? ReEPoRT OF COMMITTEE ON HISTORY Dr. J. W. Apams: Two years ago a committee was appointed by the Chair to write the early history of this Association and to fill in the gap up to the time when our first minutes appeared in printed form. During the first year of the committee’s history quite a little work was done, but no systematic work until a year - PROCEEDINGS OF A: V. M. A. 235. ago, when I was reappointed chairman, and since that time I have attempted at every opportunity that I had to write such a history. The nature of the work is such that it has to be carried on by one man. The committee that is with me is perfectly willing, but it is impossible to get the data that we want through several members, and I have attempted to do that myself. Dur- ing the last. year I have written to members of the families of deceased founders and practitioners associated with them, and 126 letters have accumulated. I have accumulated quite a stack of newspaper articles bearing on the meetings, and a great number of private letters, and it will take some little time yet to glean from this mass of material what I am after. I am attempting to write a little biography of the men who were the founders, and an account of their professional activities, and I have accumulated quite a number of photographs of the men. At this date I can only report progress. I believe it will take about four months yet to carry that up to 1891 or 1892. There are still gaps where I have nothing to put in. That is the re- port of the work of that committee to the present time. (It was voted, on motion of Dr. Fitch, seconded by Dr. Kinsley, that the report be accepted. ) (The following additional committee reports were called for but were not ready: Committee on Anatomical Nomenclature and International Committee on Bovine Tuberculosis. ) REporT OF COMMITTEE ON ABORTION (Dr. Fitch read the report of the Committee on Abortion, as follows :) Last. year your Committee on Abortion presented as a part of its report a resolution which was unanimously adopted. This resolution was as follows: “Be it resolved, That the American Veterinary Medical Association strongly urges that larger appropriations for the investigation of bovine infectious abortion be made by Federal and State Governments and through such agencies as the National Research Council to make possible cooperative work by the institutions engaged in investigating ‘this disease.” Your committee this year has directed its efforts toward carrying out the directions of this resolution. A preliminary meeting was held in Chicago early in December. At that time it was voted that data should be collected as to what institutions were engaged in the study of this disease and what phases of the infection were being investi- gated. It was also decided to interest, if possible, the National Re- search Council in the project. Correspondence was begun with Dr. C. E. McClung, who was chairman of the Section of Biology and Agriculture of the Council, also with Dr. G. W.-McCoy, chairman of the Section on Medicine. After considerable effort a conference was secured with the representatives of the Council, your committee and a few other investigators. The Council appropriated a sum of money to defray partially the expenses of this conference. This meeting was held in Washington, D. C., August 4, 1921. As a result Dr. McCoy asked that a brief be prepared stating the economic losses resulting from this infection, its importance to the breeding industry, the in- 236 PROCEEDINGS OF A. V. M. A. vestigations now being carried on, amount being expended in such investigations, and the facts which should be known about bovine in- tectious abortion and the approximate cost of such studies. These data are now being prepared by your committee and will be submitted to Dr. McCoy some time during this month. He will in turn present them before the Interim Committee of the Council. If they approve of the project as a worthy one they will initiate efforts toward secur- ing funds for the study of this disease. These funds will probably be expended at those institutions already engaged in the study of this disease, although this is not at all mandatory. We have good reason to believe that our efforts will be successful in securing additional money to be devoted to the study of bovine infectious abortion. The investigations of the past year have not influenced us to alter or amend the report presented to the Association by the committee last year. You will recall that the report of last year consisted of 14 short paragraphs, each concerning some particular phase of the disease. Among the 14 paragraphs, as far as your committee has been able to determine, 13 have received universal approval, and only one, the first, has been reasonably criticized. The paragraph in question is that in which the disease was named “bovine infectious abortion,” and the adverse criticism is based on the fact that this name is derived from a symptom which may or may not be present. ‘The true character of the infection seems to be a placentitis. In order to meet this objection a subcommittee has been appointed. Dr. Ch. Wardell Stiles, an expert on nomenclature, has been requested to serve as a member. The other members are Dr. E. C. Schroeder and Dr. Ward Giltner. They will report to the general committee on this question some time during the coming year. We believe that next year’s report will settle this troublesome question. Another point in last year’s report which needs clarifying is that relating to the diagnosis of the disease. Another subcommittee con- sisting of Dr. J. M. Buck, Dr. G. T. Creech and Dr. W. E. Cotton has been appointed to study this question and submit a report with the definite purpose of standardization of methods and technique in order to avoid many embarrassing and confusing discrepancies. A careful study of bovine infectious abortion shows that there are many phases of the disease which are still imperfectly understood. Definite information can be obtained only by carefully conducted ex- perimental work. Because of the character of the disease and the species of animal affected, research studies are very expensive and reliable results slow to obtain. Your committee feels that all available efforts should be directed toward aiding such studies and assisting in solving the problems in connection with bovine infectious abortion, which is of the greatest economic importance to the livestock industry. C. P. FitcH, Chairman. E. C. SCHROEDER. WARD GILTNER. J. F. DE VINE. HERBERT LOTHE. PRESIDENT WuitTE: You have heard this excellent report of the committee. What is your pleasure in regard to it? (It was voted, on motion of Dr. Connaway, seconded by Dr. Adams, that the report be accepted. ) Dr. ErcuHorn: I desire, in view of the importance of this question which the committee has now under consideration and the necessity of the continuation of the work, to move that this committee be continued and a sufficient fund be provided for the work for the coming year. PROCEEDINGS OF A. V. M. A. , 237 (The motion was seconded by Dr. V. A. Moore.) Dr. Stance: I would like to ask what we mean by sufficient funds. In view of the financial situation we are facing, I think - we ought to be careful, because we must remember we have a Budget Committee that considers all expenditures of the Asso- ciation for next year, so I would like to amend that motion, unless it is pretty well understood, that the Budget Committee decide the amount. Dr. E1icHHOoRN: That was understood. Dr. Stance: With that understanding I will be glad to with- draw my amendment. (The motion was put and carried.) COMMITTEE ON INTERNATIONAL VETERINARY CONFERENCE PRESIDENT WHITE: We will hear from the Committee on In- ternational Veterinary Conference, J. R. Mohler, chairman. Dr. Monier: The secretary of the committee will make the report. Dr. E1cHHoRN : The committee has not had any occasion to do any work in the past year, for the reason that in the report of the committee of last year we pointed out that Great Britain was very anxious to have the next International Congress held in London again. Great activity has been started to reestablish the International Veterinary Council, but up to date it has not been accomplished, principally due to the fact that our esteemed colleagues, particularly of France, would not reestablish inter- course with the German veterinarians. Our last year’s report also requested that this committee be continued, not for the reason of inviting the next International Congress to the United States, but in case it should be decided to have the International Congress anywhere in Europe. This committee could resume work immediately and establish a committee for the United States to cooperate with the International Congress wherever it would be decided to have it. For this reason we believe that a committee should be continued, and it is possible, in fact it is now almost certain, that a member of this committee will visit various countries in Europe next year, and it might be well to obtain the sentiment with regard to the next International Con- ference. (It was voted, on motion of Dr. Fitch, duly seconded, that the report be accepted and the committee continued.) Reports OF COMMITTEES PRESENT WHITE: Is the Salmon Memorial Committee ready to report? (The committee was not ready to report.) (The Liautard Memorial Committee was not ready to report.) PRESIDENT WHITE: The Committee on Emblem. 238 PROCEEDINGS oF A. V. M. A. Dr. S. E. BENNETT: I am about the only member of the com- mittee present. I have not been able to do anything. I have received no replies from my inquiries. There have been two or three designs submitted from different sources. If possible, I would suggest that you appoint one or two members present _ here to meet with me and we will make the report at some other time. I move that two members present at the convention be appointed to act with me. I suggest Dr. McKenna, who has taken considerable interest and submitted designs. (The motion was seconded by Dr. Kinsley and carried. ) PRESIDENT WHITE: The Chairman will appoint two appro- priate members for this committee. The Committee on Unofficial Veterinary Remedies, Dr. H. J. Milks. Dr. H. D. BEraman: Dr. Milks has this report and I believe has not yet arrived. PRESDENT WHITE: Is Dr. Dick here, who is our representa- tive on the Board of Managers of the Horse Association of America? SECRETARY Mayo: In connection with that, I think the Asso- ciation ought to know that this Association is a member of the Horse Association of America. They bought ten shares of stock last year at the Columbus meeting, costing $50. I supposed when we bought the stock that was all there was to it. It seems that when you buy $50 worth of stock you agree to pay $50 a year. That has been paid. I looked up and found we obligated ourselves, and I am sure the Association is willing to put that into it. Dr. Jacos: I believe that is for a period of three years. Secretary Mayo: I think you will be interested to know that Dr. Santa Maria, who was elected to membership, is the official representative of the Mexican Government to this meeting. (Applause. ) Adjournment. (Proceedings to be continued.) President Kinsley has appointed Dr. Cassius Way, of New York City, as member at large on the Executive Board of the A. V. M. A., to fill the vacaney made by Dr. Kinsley’s accepting the presidency of the association. Dr. Way has resigned from the Committee on Intelligence and Education. President Kinsley has appointed Dr. James Fleming a mem- ber of the Committee on Intelligence and Edueation, to succeed Dr. L. Enos Day, and Dr. J. S. Koen to fill the unexpired term due to the resignation of Dr. Way. ae 7) AL, eee ‘bene Cae ie EF TE ee er ASSOCIATION NEWS DENVER DOINGS At the Wild West show at the foot of the Rocky Mountains, after numerous Westerners had been thrown from an outlaw broncho, the manager announced by megaphone before the grandstand that he had been requested to call for Col. David S. White, the champion broncho-buster of Ohio, to undertake to ride the outlaw. This was the only instance during the meet- ing when anyone succeeded in ‘‘getting the goat’’ of our effusive and irrepressible president. The horse has not been driven from streets and farms by the auto, declares a report submitted by the Horse Association of America to the Denver Convention. The report stated that horse equipment costs less than that for motors and that truck- ing companies and farmers find that certain parts of their work ean be better done by horses than by motors. The result is that the demand for draft horses is found to be inereasing. To show this, Chicago was quoted as a representative example of American cities. In Chicago in 1920 there were approxi- mately 30,000 horses in business, and in 1921 there are 33,000. The report disclosed a nation-wide campaign by the association to bring horses back into popularity, and also to increase the production of horses, which shows a startling decrease for the last three years. On the door of a restaurant frequented by many of the con- ventioners was a sign reading: ‘‘Let your conscience be your guide; pay the cashier before leaving.”’ Major John H. Blattenberg was greatly missed at the con- vention, as the Westerners had planned to pull off a jack-rabbit game and a snipe drive, with the Major as master of ceremonies. A Missouri delegate to the convention reported seeing a woman, who had just come from the municipal market, with a live chicken under her arm, stop to give it a drink at a sani- tary drinking fountain near the convention hall. 240 ASSOCIATION NEWS The only accident which happened during the famous auto- mobile trip to Lookout Mountain was the loss of Major Cotton’s cap, when a gust of wind from Bear Creek Canyon sent it fly- ing down the roadside. A number of Eastern members hired an automobile one moon- light night and visited the coyote cage in the Denver City Park, thinking that a few yelps of the coyotes would make their West- ern trip more realistic. Report has it that notwithstanding much coaxing, the coyotes refused to comply. During the homeward trip from the grave of ‘‘ Buffalo Bill’’ the trained eye of Dr. Edmund W. Weber, of Utah, enabled him to locate a drove of elk browsing on the side of Bear Moun- tain, a half mile or more away. Commissioner John M. Whittlesey, of Connecticut, was one of the most interested delegates at the convention. Much of his spare time was devoted to the study of the agricultural con- ditions in the vicinity of Denver, including garbage feeding, hog ranches, purebred cattle farms, certified milk dairies, and chicken ranches. Unfortunately, in the absence of its chairman, Dr, E. H. Shep- ard, there was no meeting of the Practitioners’ Club, which was organized at the Columbus Convention last year. The only incident that marred the otherwise pleasant journey of the Convention Special, which left Chicago on the night of September 1, was the arrest of two of our fellow members for playing an innocent game of cards in Nebraska on the Sab- bath. A ‘‘sheriff’’ suddenly appeared in the Pullman and an- nounced the breaking of the law of the State and proceeded to take the two members to the platform of the car awaiting the stop at the next station. Much pleading and promising were without avail, and the ruse did not become apparent until an- other veterinarian who happened by and inquired as to the cause of the commotion, recognized the so-called sheriff as Dr. David S. Jaffray, a veterinary practitioner of Chicago. ASSOCIATION NEWS 241 THE WOMEN’S AUXILIARY TO THE A. V. M. A. The fifth annual meeting of the Women’s Auxiliary to the A. V. M. A. was held in the Magnolia Room of the Albany Hotel in Denver, Tuesday, September 6, 1921. The President, Mrs. A. T. Kinsley, Kansas City, Mo., pre- sided. A short, interesting program was given, the main fea- tures being the President’s address and a paper by Mrs. J. P. Turner of Washington, D. C., telling of the assistance the Auxiliary funds had given in that city. A telegram of good wishes for a successful meeting from the former President, Mrs. W. H. Hoskins, was read. The new members were accepted before the business meeting. A revised constitution was presented and accepted, wita minor changes. This incorporates in the object of the Aux- iiary (Article Il) a loan fund for needy veterinary students as well as financial assistance to veterinarians or their families. ‘ The report of the Loan Fund Committee was read and proposition No. 1 was adopted with minor changes, so that, beginning July 1, 1922, the Auxiliary offers to lend to a senior in attendance at one of the recognized veterinary schools in the United States or Canada a sum not to exceed $350 at 4 per cent interest, subject to the conditions suggested by the committee. A telegram of sympathy in her recent bereavement was sent to the ex-President, Mrs. W. H. Hoskins, and the following resolutions were adopted: Whereas, God, in His infinite wisdom, has removed from our midst Dr. W. Horace Hoskins, the beloved husband of our most loyal and faithful member, the originator and first presi- dent of our Auxiliary; and Whereas, Dr. Hoskins always showed the keenest interest in the affairs of our Auxiliary and was ready to help with advice and counsel; Therefore be it Resolved, That we, as members of the Women’s Auxiliary to the American Veterinary Medical As- sociation, extend to Mrs. Hoskins our heartfelt sympathy in her grief, And be it further Resolved, That the Secretary be in- ‘structed to send a copy of these resolutions to the bereaved 242 ASSOCIATION NEWS member, Mrs. W. H. Hoskins, and that these resolutions be spread upon the minutes of this meeting. Mrs. C. E. Corron, Chairman. Mrs. I. A. JOHNSON. Mrs. N. 8. Mayo. As the tenure of office is two years there was no election at this meeting and the Auxiliary was adjourned to meet during the next annual meeting of the A. V. M. A. MaArGuERITE M. LockHart, Secretary. A. V. M. A. ELECTION NOTICE Postal ecards for nominations for a member of the Executive Board for District No. 6 will be sent out from the Secretary’s office soon. District No. 6 comprises California, Nevada, Utah, Colorado, Kansas, Missouri, Arkansas, Oklahoma, Louisiana, Texas, New Mexico, Arizona, Mexico and Central America. According to the Constitution and By-Laws, no member can vote who has not paid his dues for the current year. All members of the A. V. M. A. who live in this district and who have paid their dues will receive both nominating cards and regular ballots. N. S. Mayo, Secretary. HELP WANTED At the next meeting of the Executive Board of the A. V. M. A. that will probably be held in Chicago about the first of December, plans will be considered for increasing the membership of the A. V. M. A. The Executive Board will greatly appreciate sug- gestions from the individual members as to how our membership can best be increased. If you have any suggestions send them to me and they will be presented to the Board. N. S. Mayo. DUES ARE DUE About the middle of August statements for dues were sent to every member together with a program of the annual meeting. Quite a number have not paid their dues for the year 1920-21. If “you have not sent a remittance, please do so now. The dues are payable in advance and if they are not paid promptly, it will be necessary to stop your JOURNAL. Please send your dues now to the Secretary. Perinat ren Om , . elptina sae P-Oer Par onse ASSOCIATION NEWS 243 NEW YORK STATE VETERINARY MEDICAL SOCIETY THE thirty-first annual meeting of the New York State Veterinary Medical Society was held at the Lafayette Hotel, _ Buffalo, July 27, 28 and 29. The meeting was called to order by the President, Dr. Wright J. Smith, at 10 a.m. The repre- sentative from the Mayor’s office who was to give the address of welcome had to leave before the meeting was opened and that feature was deferred until the afternoon session. The entire morning session was used in carrying out the order of business up to the reading of papers. At the opening of the afternoon session Mr. Sweeney, on behalf of Mayor Buck, gave the address of welcome. Dr. Chas. 8. Chase of Bay Shore gave a very fitting response to Mr. Sweeney. Dr. E. Sunderville read a paper for Dr. L. A. Norget on the “‘Importance of Correct Diagnosis.’’ Dr. Norget was present but just recovering from a very severe attack of tonsilitis he was neither able to read his paper nor take part in the interesting discussion which followed. Dr. P. A. Fish read the next paper. It was entitled ‘‘The Spermatic Secre- tion.’’ Discussion was opened by Dr. W. W. Williams of Springfield, Mass. Dr. W. Reid Blair read an excellent paper on ‘‘Hookworm Disease in Dogs.’’ Dr. H. J. Milks led the discussion on this paper. The annual dinner of the society was held at the Lafayette Hotel. Dr. V. A. Moore gave an instructive and helpful talk -on the veterinary profession. Drs. Wills, Hollingworth, Blair, DeVine and Volgenau were called upon for discussion. These men sustained the interest in the topic and helped to -make this part of the program one of the best features of the meeting. The first session of the second day was given up to papers by Dr. W. W. Williams and Dr. W. L. Williams. The paper by Dr. W. W. Williams was on ‘‘Observations upon Reproduc- tion in a Purebred Dairy Herd.’’ The one by Dr. W. L. Wil- lams was on ‘‘Observations upon Reproduction in a Purebred Beef Herd.’’ These were interesting and valuable papers and they, with the discussions which followed, took up the whole - of the morning session. The papers of the afternoon were by Dr. J. W. Benner, Dr. _W. G. Hollingworth and Dr. C. J. Spencer. The paper by Dr. 244 ASSOCIATION NEWS Benner was on *‘ Mixed Infection in Swine.’’ The title of the paper by Dr. Hollingworth was ‘‘Am I an Asset or Liability ?’’ Dr. C. J. Spencer talked on the subject ‘‘A Few Ideas of an Ordinary Practitioner.’ Dr. Spencer illustrated his talk by several appliances which have been used with success in his own practice. The paper by Dr. Hollingworth was rich in the philosophy of an honorable and successful life in veterinary practice. Its teachings are those toward which every man in the veterinary profession should look. Dr. D. H. Udall of Ithaca was elected President for the en- suing year; Dr. W. Reid Blair was elected Vice-President; Dr. C. E. Hayden, Ithaca, Secretary-Treasurer, and Dr. H. J. Milks, Ithaca, Librarian. Twenty-four new members were elected during the meeting. There is a steady and substan- tial growth which marks an increase in the influence of the organization. It was voted to send a telegram of sympathy to Dr. W. Horace Hoskins who, it has since proven, was in his last illness. Resolutions were presented on the death of Dr. D. W. Cochran, Dr. James Law and Dr. W. S. Eggleston. It was voted ‘to hold the next annual meeting at Syracuse some time during the summer of 1922. The invitotion was extended by Dr. A. E. Merry on behalf of the Central New York Society. The local Committee on Arrangements provided a boat ride to Crystal Beach as the entertainment for the evening of the second day. Crystal Beach is one of the most popular amuse- ment places which Buffalo offers. A large number of members and visitors took advantage of the opportunity to visit Crystal Beach and found it an excellent place to have a good time. During the morning of the third day a visit to the Stock Yards, Dold’s Abattoir and the Crandall Sales Stables was made. There was opportunity to see the regular operations in the Dold plant. An instructive exhibit of pathological speci- mens had been prepared by the staff and there was much interest manifested in it. Lunch in the Dold dining rooms was an enjoyable feature of this visit. In the afternoon a large number of members and visitors took an automobile trip to Niagara Falls. A well-deserved vote of thanks was extended to the members of the local Committee on Arrangements for their splendid and effective work in caring for the meeting. C. E. Haypen, Secretary. * Van deel ASSOCIATION NEWS 245 WESTERN MICHIGAN ASSOCIATION The summer meeting of the Western Michigan Veterinary Medical Association was held at Manhattan Beach, Reeds Lake, Grand Rapids, Mich., August 16, about 40 being in attendance. An excellent entertainment program was enjoyed by all present. New members accepted at this meeting were Drs. B. A. Perry of Hastings and R. C. Rawlings of Caledonia. O. H. Van Brussex, Secretary. U. OF P. VETERINARY ALUMNI DINNER Following the ancient custom of all loyal sons of Old Penn, graduates of the Veterinary School of the University of Penn- sylvania never fai! to ‘‘gather ’round the congenial and festive board’’ whenever a few of the sons find themselves together. True to Old Penn tradition every Penn man attending the recent A. V. M. A. meeting, held in Denver, attended the ‘‘Pennsylvania dinner’’ held Tuesday evening, September 6, at the Albany Hotel. After a very enjoyable repast each man present told what he knew of the activities of the members of his class. Many very interesting reminiscences were indulged in and thor- oughly enjoyed by all. Dr. Chas. E. Cotton of Minneapolis presided, and the follow- ing submitted ‘‘reports’’: Dr. John W. Adams, Veterinary Sehool, U. of P.; Dr. John R. Mohler, Washington, D. C.; Dr. T. EK. Munee, Harrisburg, Pa.; Dr. H. P. Hoskins, Detroit, Mich.; Dr. H. W. Jakeman, Indianapolis, Ind.; Dr. M. Jacob, Knoxville, .Tenn.; Dr. N. L. Townsend, New York City; Dr. Howard H. Custis, La Jara, Colo.; Dr. F. S. Jones, Princeton, N. J.; Dr. C. S. Shore, Lake City, Minn.; Dr. R. M. Staley, Philadelphia, Pa. Dr. Munce reviewed briefly the report submitted by the Welfare Committee at the June, 1921, meeting of the Alumni Society. He told of the good spirit shown by the alumni body and particularly the Welfare Committee, during their meet- ings and discussions. Also of the valuable and cordial co- operation received from the faculty of the Veterinary School. It is believed that the greatest possible good will result from the closer relationship between the school and the alumni body. 246 ASSOCIATION NEWS The enthusiastic and optimistie note that was evident in the remarks of everyone at this meeting shows that every Penn man is for Penn. Dr. Adams reviewed the work at the school during the past- few years, and told us that the prospects for a larger freshman class are better than they have been, at this season, since prior to the war. Great satisfaction was expressed that the clinical material presented daily at the free clinic held at the Veterinary School is very abundant and includes practically all domestic animals. The work of the ambulatory clinic, which takes the students to some of the greatest dairy and livestock breeding establish- ments in the country, was described as one of the best con- ducted ambulatory clinics in operation. It is under the super- vision of Dr. C. J. Marshall, who is giving the students the benefit of his years of }:ractical experience, in conjunction with his course in Veterinary Medicine. As proof that the present teaching methods and ambulatory clinies are fitting men to enter present day general practice it was recalled that, at the 1920 Alumni Day exercises, all of the clinical work was carried out by senior students, who accomplished the work like seasoned practitioners. The meeting adjourned with a toast—an exceedingly dry toast—to Old Penn and the continued success of the Veteri- nary School. R. M. Srarey, Secretary. GEORGIA VETERINARY ASSOCIATION The fifteenth annual meeting of the Georgia Veterinary Asso- ciation held in Macon, Georgia, September 21 and 22, passes into history as one of the most successful meetings ever held by this association. In spite of the fact that financial conditions in the South have been very discouraging during the past year, a larger number of veterinarians were present than have been in any meeting of the past five years. The meeting, held at the Hotel Lanier, was called to order on the morning of the 21st by President Dr. B. E. Carlisle, of Montezuma, Georgia, and a welcome on behalf of the city of Macon was extended to the association by Roger Miller, Secre- tary of the Macon Chamber of Commerce. This welcome was responded to by Dr. John I. Handley of Atlanta. MiRee ick dadeke eel oe ee pine aR Sy eeary Le ASSOCIATION NEWS 247 The first session of the meeting was devoted to the discussion of the hog cholera question. The first paper, ‘‘ Parasitic Infes- tation in Its Relation to Hog Cholera,’’ given by Dr. Guy T. Cole, U. S. Inspector in charge of meat inspection at Moultrie, Georgia, was a revelation in that it showed that parasitic infes- tation of hogs causes more condemnation of hog careasses than all other diseases combined. Thousands of pounds of pork are condemned in the packing plants; and if conditions seen in a small packing house are indicative of the country generally, millions of dollars are lost annually due to the needless harbor- _ Ing of parasites by the hogs on the farm. A motion was made at this time to give this paper as wide publicity as possible through the press of the State. The next paper, ‘‘Intra-peritoneal Injection of Hog Cholera Serum Compared with Other Methods of Injection,’’ by Dr. J. H. Coffman, Assistant State Veterinarian, Atlanta, was a short history of the vaccination of hogs against cholera. This paper brought out the fact that due to the ease of the operation, the absence of abscesses and the readiness with which the serum is absorbed, the intra-peritoneal method of injecting hog cholera serum is far superior to all other methods. A short review of losses following the serum simultaneous treatment of hogs was given by Dr. D. L. Proctor, Hawkins- ville, in his paper ‘‘Hog Cholera? If not, What?’’ Dr. Proctor reported that the herds under discussion had been treated and retreated with serum and virus alone and in conjunction with bacterins and, though the ante-mortem symptoms and _post- mortem lesions were those usually found in hog cholera, the disease could not be checked. This paper brought out an abundance of opinions as to the cause of these losses and the real identity of the disease. ' ‘Indications and Contra-indications for the Use of Bac- terins’’ was well handled by Dr. J. l. Ruble of Quitman, who showed, by citing cases in his hog practice, that there are con- ditions in which bacterins are indicated. The afternoon session of the first day was given over to the discussion of subjects pertaining to general practice. Dr. G. W. Browning, LaGrange, read a paper on ‘‘Some Things We Should Not Do in Canine Practice.’? He pointed out the fact that care should be taken in administering drugs to puppies, and showed 248 ASSOCIATION NEWS that simple remedies were often more effective than poisonous drugs. This paper brought out some interesting discussions along the line of canine practice. Following this paper, Dr. T. F. Abercrombie, Secretary of the State Board of Health, Atlanta, gave a report of the work being done by the State Board of Health Laboratory in the control of rabies in Georgia. The figures given by Dr. Abercrombie showed that rabies is on the increase in this State and as a result of this report, a committee was appointed, consisting of Drs. Bahnsen, Americus, and Burkland, Atlanta; Drs. Richardson and Pureells, Athens, and Dr. E. D. King, Jr., Valdosta, to investigate experiments con- ducted by the Board of Health Laboratory and if results were satisfactory, to recommend to the General Assembly of Georgia at their next meeting, the passage of laws controlling dogs with a view of lessening the occurrence of this disease. Following the discussion of this report, Dr. John W. Salter, Dawson, gave an interesting paper on the pathology of and the technic of the operation against roaring in horses and mules. Dr. P. W. Hudson, Americus, then related some of his experi- ences with parturient paresis. This paper brought out a variety of arguments for and against the use of different drugs to be given in connection with the inflation of the udder in treating these cases. The final paper in this session was given by Dr. E. L. Jarvis of Macon, on the subject of abortion. Dr. Jarvis. gave an in- teresting review of his experiences with this disease in cattle. He considered the use of bacterins very satisfactory in treating herds of cattle infected with contagious abortion. The discus- sion following this paper was participated in by a number of veterinarians present, and brought out the fact that abortion is present in Georgia, not only among the cattle, but also in herds of hogs. The evening of the first day was spent around the banquet table. Due to the absence of Dr. M. A. Morris, Dr. Bahnsen acted as toastmaster and a memorable evening was enjoyed by all present. The morning of the second day was given over to the subjects of municipal meat and milk inspection. Dr. E. D. King, Jr., City Meat and Milk Inspector, Valdosta, in his paper, “‘Is Pub- lic Sentiment a Factor in Establishing Meat and Milk Inspec- * hee ASSOCIATION NEWS 249 tion?’’ showed that no public work can be successfully carried out without the backing of the public. In his opinion, the public should be educated as thoroughly in the relation of contaminated and diseased milk and meats to the health of a community as it is in the branches of the common school. ‘“Why Should a Veterinarian Be in Charge of Municipal Meat and Milk Inspection?’’ was well handled by Dr. A. G. G. Richardson, Dean of the Veterinary Division, State College of Agriculture, Athens. Dr. Richardson, who for many years was a B. A. I. Inspector, showed that the veterinarian is the only man who can control the sanitation of meats and milk from the farm to the consumer. He also showed that no lay inspector can determine the health and condition of the animal or carcass before its products are offered for public consumption, and that few lay inspectors are qualified as sanitary officers. Dr. R. K. Roberson, Milk Inspector of Columbus, Georgia, on ‘‘Dairy Sanitation,’’ gave the points to be considered in conducting a sanitary dairy and the production of wholesome milk. This paper brought out an interesting discussion. The afternoon session was to have been a demonstration of the combination test for tuberculosis, but as no reactors were found, this part.of the program was substituted for a clinic held in Dr. Jarvis’ hospital. The business session closed the meeting. Dr. A. L. Hirleman, U. 8. Veterinary Inspector in charge of cooperative hog cholera and tuberculosis control, Atlanta, was elected President for ihe coming year, with Dr. A. G. G. Richardson, Athens, Vice-P resi- dent. Dr. Peter F. Bahnsen, Americus, was unanimously re- elected Secretary-Treasurer. Upon invitation of the Chamber of Commerce and Drs. Epple and Toliver of Albany, that city was awarded the meeting of the association for the year 1922. It was voted that the next meeting be held between the fifteenth and thirtieth of September. , Perrer F. BAHNSEN, Secretary. MISSISSIPPI] DELTA VETERINARY ASSOCIATION The Mississippi Delta Veterinary Association held its regular quarterly meeting in Cleveland, Miss., on September 14, the sessions being held in the Firemen’s Hall. The meeting was 250 ASSOCIATION NEWS ealled to order at 10 o’clock, with Dr. C. D. Crawford, presi-- dent of the association, in the chair. Papers were read and general discussions held on the follow- ing subjects: ‘‘Periodie Ophthalmia,’’ Dr. S. E. Osborn, of Greenwood; ‘‘Black Tongue in Dogs,’’ Dr. Luster, Clarksdale; ‘“Swamp Fever,’’ Dr. Sullivan, Sumner; ‘‘Rabies,’’ Miss Goach, of the Bolivar County Health Department ; ‘‘Hog Cholera,’’ Dr. Fry, Jackson; and ‘‘ Anthrax,’’ Dr. Norton, of Greenville. The association has asked the leading laboratories to study the question of a stronger vaccine for anthrax, and a commit- tee of veterinarians reported to the association that the labora- tories are now working on a product which will give permanent immunity. The veterinarians in attendance were: Drs. C. D. Crawford, Rolling Fork, president; Bannester, Greenwood, vice presi- dent; E. B. Mount, Cleveland, secretary-treasurer; Fry, Jackson; Nye, Grenada; Clark, Grenada; W. S. Sullivan, Sum- ner; J. C. Boyce, Tutwiler; C. Stallworth, Drew; Luster, Clarks- dale; R. H. Mohlenhoff, Cleveland ; Osborne, Greenwood ; Norton, Greenville; Berry, Leland; Royal, Hollandale; and Heath, of Grenada. E. B. Mount, Secretary, NEW VETERINARY CLUB ORGANIZED A meeting of veterinarians of seven Central Pennsylvania. counties was held recently at the home of Dr. H. T. McNeal, Sunbury, Pa., and a.permanent organization, to. be known as the Central Pennsylvania Veterinary Club, was formed. The club, which consists of veterinarians of Northumberland, Columbia, Montour, Snyder, Union, Juniata and Mifflin counties, was organized to form a closer personal and professional rela- tion among the members, as well as for the discussion of sub- jects pertaining to the profession and otherwise advancing the interests of its members. The meeting was well attended by veterinarians throughout the district. Dr. H. R. Church, of Harrisburg, deputy State veterinarian, gave an interesting talk. Dr. B. M. Potteiger, of Selinsgrove, was elected secretary. The next meeting will be held at Bloomsburg, and Dr. Wm. Smith, of Stillwater, Columbia County, was named president for that session. Meetings will be held every three months and a president for each meeting will be named from the section in which the meetings are held. shhieeel Sie ee . Te \ ASSOCIATION NEWS 251 MONTREAL VETERINARY CONVENTION The Quebee Veterinary Medical Association, officially known under the name of College of Veterinary Surgeonsofthe Province of Quebec, will hold its annual convention early in November, when a veterinary surgeon officially delegated by the Société de Médecine Pratique, of Paris, France, is expected to be present to perform surgical work and possibly read one or two papers. Owing to the uncertainty of the date of sailing of the French veterinary surgeon, it has been found impossible to set defi- nitely the date of the convention, which will take place either the latter part of October or the very beginning of November. Those who have had the good fortune of being present at former conventions will probably give the incredulous look when they read that the forthcoming convention will eclipse any- thing ever attempted before by the Montrealers, but the offi- cers have the doggy determination which spells success with a capital S, and, since they have never before failed to keep their word, the convention is bound to be heard of favorably later on. The demonstrations of the intended delegate from France will take the almost if not the altogether exclusive form of sur- gical work, and many are the names of prominent veterinary surgeons throughout the country who have signified their in- tention of being present. The committee wishes to extend a hearty invitation to all vet- erinary surgeons outside the scope of the association to be pres- ent as guests, those of Quebec having already received a circular letter requesting them to attend as active members and asking for their cooperation. The convention will take place at the Montreal Veterinary College, under the chairmanship of Dr. Albert Dauth, with Dr. J. H. Villeneuve as secretary-treasurer of the Organization Committee, at 266 Craig Street East, Montreal. J. H. VitLENEUVE, Secretary. THE PARIS VETERINARY CONFERENCE Belated reports of the Paris conference from several of the French veterinary periodicals indicate that forty-three countries (including dominions, colonies, ete.) were represented by delegates. Germany was represented by Prof. Robert Ostertag and two others. 252 ASSOCIATION NEWS Sir Stewart Stockman represented Great Britain and also Canada and was chairman of a committee. The French delegation of eight included Doctors Galmistie, Leclainche, Roux and Vallée. Sir Arnold Theiler represented South Africa and Dr. W. H. Wray the United States. Prof. Hutyra was there representing Hungary, while Dr. DeJong was the delegate from the Nether- lands. Every country in Europe except Russia and Turkey seems to have been represented ; also Brazil, Chile, Paraguay, Peru, Aus- tralia, New Zealand and Japan. The president of the conference was a French Senator, Massé. The details of this conference will be found in the October, 1921, JOURNAL. THE FIRST WORLD’S POULTRY CONGRESS The first World’s Poultry Congress was held at The Hague, Holland, September 5 to 15, 1921, Queen Wilhelmina and the Prince Consort opening the Congress. ‘The work was divided into two parts, viz., that of papers, and that of an ex- hibition. Of interest to veterinarians was the exhibition, which comprised a collection of pathological specimens of fowls, nicely mounted and staged by the State Serum Institute of Rotterdam, inelud- ing specimens of tuberculosis, roup, diphtherie enteritis, para- sites, etc. The other section on disease was by the Poultry Pathology Research Laboratory of the North Carolina Experi- ment Station and included enlarged photographs, photomi- crographs and prepared specimens of tumors of fowls and black- head of turkeys, ete. The program of papers was divided into sections, the third being that of Hygiene and Disease, which was presided over by Dr. D. A. De Jong of the State University of Leyden. Twelve papers were presented in this section by veterinarians who have specialized in diseases of poultry. Among the contributors were Prof. Dr. J. Poels, Professor Dechambre, Dr. D. A. De Jong, Dr. B. F. Kaupp, Prof. Dr. L. Le Blieck, Dr. J. R. Beach, Dr. F, Van Heelsbergen, Dr. B, J, ©, Te Hennepe, Dr. H. Van bo Or Co ASSOCIATION NEWS Straaten, Dr. H. E. Reeser, Dr. L. F. Rettger and Prof. Dr. Camillo Terni. Among the resolutions passed two are of especial interest to the veterinarian : ° 1. In all countries where poultry production is an important industry, that adequate courses be provided in the teaching of poultry diseases in all veterinary and agricultural colleges. 2. In all countries where poultry production is an important industry, that investigation of diseases of poultry be continued and a study of the advisability of official control of outbreaks of contagious and infectious diseases among poultry be made, and in the event of the advisability of such control, to determine whieh of the contagious diseases should be so controlled. One of the excursion trips was to Rotterdam to visit the State Serum Institute. This institute conducts studies of diseases of the domestic animals of Holland, including poultry. It also maintains a laboratory for the examination of milk and water. It produces the sera and vaccines used in Holland for both prophylactic and curative measures in combating diseases of livestock and poultry. In this part of the institution there are kept 150 horses, 60 cattle and 200 hogs. The sera used on cattle are made from hyperimmunized cattle. It was found that some cattle suffered ‘‘serum disease’’ when sera from horses were used. The sera used in combating diseases of poultry included one for fowl cholera and one for fowl typhoid. B. F. Kavupp. TUBERCULOSIS ERADICATION CONFERENCE The Tuberculosis Eradication Conference, which will be held in Chicago, November 25 and 26, 1921, promises to be the most important meeting of its kind ever held in the United Stafes. The program which appears below contains the names of many of the best authorities in their respective lines in the United States. Every person assigned to the program has a close con- nection with the tuberculosis eradication problem either from the human or livestock side. If there is not sufficient time to discuss all of the subjects thoroughly during the two days of the con- ference, it will be extended over another day. _— ee =) = = MAAMSERMANAMOUMNVAN iS) Keeee & eeveecesces ree ia} ne 5 2 | 2] & 5 = = mMeSSeuraqanunssconn a & ANNANANANNSRA=RRAANN — | i = ' ° z = ANAMAIAMSOCHUMUKNS = = Cecoooeerrrrrerr S me = il n S | — s Zule Subas S| = = DABroenonmeneoncors > & NANAASASNSRORRHOH bast cS n at fe ° a = AMPAMSAGSPCOSANA 5 m & escooesceeececececen Da 5 3 : = 3 Boies |e = g = PSPS OCOMENCKHSCEEEY n > ABNNANSANNANAAAA =| | = n = Ss e& 3 2 8 = POreowuratonssso l 5 eescoeeecernrreenr ai z s la! ° eS = cd om a aS 3 SHLOOCACUEMNSCASSSS e | aS ee I NAO MOCOmeOoR —— J [ont AAAI AA AA et DUD EDD | & Soeeececeeececenn z wt ° s c Bi AS fa = Cersoneannvesraaen i NANSANAANANAQAQANAQAAGN aad al Number ol 276 F. S. JONES The two remaining cultures (Bov. Pn. 1 and 9) fall into a distinct group. Media containing dextrose, saccharose and mannit are acidulated. No fermentation occurs in lactose, mal- tose or salicin broth, although alkali is produced under suit- able conditions in such tubes. Both strains form indol. They do not hemolyze horse red blood cells and they are insoluble in bile. Recently all strains were tested for inulin fermentation. In no instance was acid produced. Pathogenicity—The members of Group I are of relatively low virulence for laboratory animals. One-fourth of a cubic centimeter of freshly isolated 24-hour bouillon cultures when injected beneath the skin of rabbits produced only mild local reactions. A calf injected in the subcutis with the pleural exudate from a cow dying of pneumonia developed only a slight local reaction. Intratracheal injection of a calf with 10 ec. of bouillon culture failed to cause marked disturbances. Group II cultures are slightly more virulent for mice and rab- bits. One-fourth of a cubic centimeter of a 24-hour bouillon culture injected beneath the skin of a rabbit usually causes a marked local inflammation which may be absorbed or may terminate in an abscess. Culture Bov. Pn. I of Group III, al- though under cultivation since 1913, is still virulent. Rabbits die within three or four days when injected subcutaneously. Reference has been made to earlier attempts to determine immunological relationship between various members of the pasteurella group by means of various protection tests. The agglutination test has in recent years become well recognized as a method of grouping organisms. Accounts of its use in separating the pasteurella have not been met with. Magnusson repeatedly injected a sheep with a culture of the reindeer or- ganism but was unable to demonstrate agglutinins. Shirop makes no mention of agglutinins in the blood of rabbits im- munized with Bacillus vitulisepticus. Hadley (8) succeeded in producing a serum by injecting a rabbit with B. avisepticus. The serum agglutinated the homologous culture at a dilution of 1: 160. It was decided to attempt to group the organisms serologi- cally by means of their agglutination affinities. Rabbits were immunized with a single strain chosen from each group. It 277 Types oF B. Boviserricus IN Datry HERD VE) (PEAT Wea SL FO Pat PI 000°'T:T (pl belly) Telly er EE 00S'T Wet LS SST ore 00%:T Led bed ae LPS. 91 at sli Pail et a ap he} OOLT 1 100 PST a aa RreS NLS) UP SUS te ies DL De TPL Lee US ela Then bea kere) eS Se AE oils Vata I+ OS'T | 0G:T | OTT | 00S:T (eT. IN eae ESP | 00G:T bitiriif$$oror OOLT ee hOOOS aie | igkeh li) LSOuUSs Oi I 0aT + 111 lt+4 OOOOF OU] | 00S: T Fd oe (9 dn | DOOVVVVSS!IIIII IL! OOVSSOVOVOSO!lIIIIIi | 00Z:T | OOT?T | OS?T OVVSVVOSO!lIIIIIII I OVVOVVOSOO! I! III ITI OT O€S ‘Lug ‘Aog 91nqjnD [{][ dnory yA pozranuran yiqqey SuOI}NTIp wunseg suONNyIp uNnsesg suor nip wMNseg TEP ANAND J] dnosy YIM pozranuwull yiqqey ogs eangnD | dnosry yy pozrunuran wqqey ‘vuaS dNOUX) IVYALIND ISNIVOY SNIVULS T1IY dO SLSa], NOILVYNELATISDY AO SLTASAY—E ATAVL seiny[ng 278 F. 8. JONES soon became apparent that the rabbits receiving Group I and Group II strains developed agglutinins slowly. Individuals receiving a single Group III culture produced agglutinins much more rapidly. Tests were made from time to time; when it seemed that the maximum titer was reached the animals were bled. Each individual’s serum was tested against all strains. The results are recorded in Table 3. It may be mentioned that the best results were obtained when the growths from 24-hour slant agar cultures suspended in salt solution were used as antigens. The density of the suspen- sions was about 2.5 with the Gates’s (9) apparatus. All tubes were incubated at 38 degrees C. for 18 hours. Groups I and II agglutinate slowly ; clumps are rarely visible before the sixth or eighth hour. Agglutination of the Group III organisms takes place rapidly ; clumps begin to form in a few minutes, and all the organisms are usually down within 6 or 8 hours. From Table 3 it will be noted that the serological grouping follows the cultural grouping to a considerable degree. All strains of Group I are agglutinated well up to the titer limits with Group I serum. All strains of Group II, except 502, are likewise agglutinated by their homologous group serum. The same holds true for Group III. In no instance has cross ag- glutination taken place. When the serum is absorbed by a single group strain the agglutinins are uniformly absorbed for all members of the group. Strain 502, although possessing cultural characters in com- mon with Group II, fails to excite the same immunological process. Dochez and Gillespie (10) have brought out the fact that among the pneumococci distinct serological differences exist among organisms having cultural characters in common. The production of a polyvalent serum was next attempted. Rabbits were immunized with suspensions of cultures 530, 482, 502 and Boy. Pn. I. As in the previous observations, strong agglutinins for strain Bov. Pn. I developed rapidly. Tests against culture 530 showed moderate agglutinin formation. The agglutinins were not as strong for culture 432. Appar- ently strain 502 excites but little agglutinin formation in the rabbit, since it was incompletely clumped only in the lowest dilutions (1:5 and 1:10). Types oF B. Bovisepricus In Dairy Herp 279 DISCUSSION It might be well to emphasize some of the more important points brought out by these observations.. That the term Bacillus bovisepticus can no longer be taken to represent a single strain or species of organism seems clear. From the limited material available it has been possible to recognize three distinct cultural groups. Perhaps a study of a greater number of cultures may disclose a larger number of aggrega- tions. In fact at this time another culture has been isolated which differs from any of those described. Of still more im- portance to those concerned in the study of animal diseases are the serological differences manifested. In the main the sero- logical grouping follows the cultural grouping. A single strain has produced agglutinins for all other strains with similar characters. This has held true with a single exception. Per- haps a study of more strains may disclose more immunological groups. These facts must be taken into account when attempts are made to control outbreaks with antisera or vaccines. Through a knowledge of the specific characters of members of the bovisepticus organisms it has been possible to trace vari- ous types of infection in a particular herd. One of the first strains isolated (9) was obtained in February, 1917. This is the only Group III organism cultivated from this herd. Early in 1917 a Group II culture (6) was isolated by Dr. Smith. During the years 1918, 1919, 1920, and in January, 1921, he found such organisms in calves associated with primary infec- tons with B. actinoides. Evidently this type had become endemic. During the later months of 1920 a fresh virus (Group I) was introduced with newly purchased cows. A small out- break promptly ensued. This subsided, but sporadic cases con- tinued to develop in both the adults and calves. This organ- ism has become the predominating type. Future studies will determine whether or not it will entirely replace the preceding types and in turn be replaced by a freshly introduced virus. SUMMARY Sixteen organisms isolated from cases of pneumonia occur- ring in cows and calves have been studied. Morphologically all cultures-resemble each other. It has been possible by study- ing their fermentation and other characters to divide them into three well-defined groups. In the main serological relation- 280 F. S. JONES ship has been found to follow the cultural grouping. A serum prepared by immunizing a rabbit with a single Group I strain agglutinates all Group I cultures.. The same is largely true for Groups II and III. In no instance has cross agglutination occurred. Absorption of a serum with a single group strain exhausts the agglutinin to the same degree for all group strains. LITERATURE CITED 1. Jones, F. S., and Littte, R. B. Jour. Expt. Med., 1921, vol. 34, p. 541. 2. SMITH, T. Jour. Expt. Med., 1921, vol. 33, p. 441. 3. SMITH, T. U.S. Dept. Agr., Bur. Anim. Indus., 12th and 138th Ann. Repts., 1895-1896, p. 119. 4. OSTERTAG, R. Ztschr. Infekt. der Haust., 1908, vol. 14, p. 1. 5. Suirop, H. Centrlbl. Bakt., etc., 1 abt. Orig., 1908, vol. 47, p. 307. 6. MAGNUSSON. Ztschr. Infekt. der Haust., 1914, vol. 15, p. 61. 7. BESEMER, A. M. Jour. Bact., 1917, vol. 2, p. 177. 8. HADLEY, PHILIP. Jour. Bact., 1918, vol. 3, p. 277. 9. GATES, F. L. Jour. Expt. Med., 1920, vol. 31, p. 105. 10. DocHEz, R., and GILLESPIE, L. F. Jour. Amer. Med. Assoc., 1913, vol. 65, p. 727. DISCUSSION Dr. Hoskins: Mr. Chairman, I am very glad Dr. Jones Was given an opportunity to present his paper. For a num- ber of years we have been trying to get somebody from the Rockefeller Institute, either Dr. Smith or Dr. Jones, to con- tribute to the program of the Section on Sanitary Science and Police. This year it appears we have been successful. Per- sonally, I am very much gratified that this opportunity was given Dr. Jones this morning. I would like to discuss the paper very briefly. I brought a paper to this meeting which was read by title in the Section on Education and Research, dealing with some investigations on bovine hemorrhagic septicemia, more specifically the shipping fever of calves, which is looked upon more as the pulmonary type of hemorrhagic septicemia in cattle. A very brief sum- mary of the contents of that paper shows that we were able to isolate two types of Bacteriwm bovisepticum from the same animal. These two types were not differentiated in the same manner that Dr. Jones has used for differentiating his types. Our two types differ more according to virulence. Of the two types which we isolated one was secured from the lungs of a ge tan, “aS ae nh Tg PRA Ae Me Ee i aoe oar’ Types oF B. Bovisepricus In Dairy Herp 281 calf dead of stocks-yards pneumonia, or shipping fever, and the other strain was isolated from the hemorrhagic muscle tissue, presumably the blood, of the same calf. The lung strain was obtained by plating. An emulsion of the same material which gave us the organism by plating failed to kill rabbits. We speak of that as our non-virulent lung strain. The other strain, obtained from the muscle, was isolated both by plating and by rabbit passage. We speak of that as our virulent muscle strain. Bear in mind that these two strains, a virulent and a non-virulent, were isolated from the carcass of the same animal. These two strains agree with either Dr. Jones’ type two or type three. They have the usually accepted fermentation reactions, dextrose positive, lactose negative, and saccharose positive. Both strains are much alike morphologi- eally and culturally, except when we come to examine bouillon cultures. There the two strains show marked differences. These agree with the differences described recently by De Kruif, in his study of rabbit septicemia, where he has ap- parently demonstrated the co-existence of virulent and non- virulent strains of the rabbit septicemia organism in the same culture. I believe we have gone one step further in isolating the two strains separately from the same animal.- Two years ago I presented a paper before the Missouri Val- ley Veterinary Association, and at that time predicted that some of these days we would be dividing or typing the hemor- rhagie septicemia organisms very much in the same manner as we are now typing the pneumococcus group. I believe that Dr. Jones has made true that prediction. I believe I will say, however, that if in isolating a hemor- rhagie septicemia organism from suspected material, we obtain an organism which corresponds to Dr. Jones’ type one, per- sonally, I would hesitate to call it a hemorrhagic septicemia organism. You will note that it ferments lactose and that it is hemolytic. Those two characteristics in themselves would prompt me to throw it out as a typical hemorrhagic septicemia organism. Also, bear in mind the difference in the agglutina- tion tests, as shown in the lower table. Note that where you take strictly true types, either two or three, and cross-aggluti- nate against type one, there is no cross-agglutination. Dr. Jones: Group one is apparently what Magnusson de- 282 F. S. JONES seribes in Lapland. The American Bacteriological Association has established a new tribe called pasteurella. Their require- ment for the hemorrhagic septicemia is that they are non- motile rods which produce a little acid in carbohydrates. They are negative to Gram stain and parasitic for animals. Cer- tainly group one can well be placed within such classification. For these reasons they have been placed with the pasteurella. They are undoubtedly what people have always assumed to be bovisepticus. Whether they are or not, time will tell. Dr. ErcHHorRN: May I ask if Dr. Jones has conducted any experiments along immunological lines? Dr. Jones: A little work along this line has been done. I can produce protecting serum for group three organism. A group two serum has thus far failed to protect against group two cultures. ANTITUBERCULOSIS VACCINATION The work of Calmette and Guérin on the vaccination of cattle against tuberculosis with cultures of bovine bacilli grown on bile and glycerin, which has been previously referred to in THE JOURNAL, is reviewed and discussed by L. Panisset in the Revue Générale de Médecine Vétérinare,? with the following conclusion : ‘“The practical value of the method of Calmette and Guérin can be determined only by extended experimentation on a large number of animals during a number of years corresponding to the mean duration of the economic life of bovines. However, in the light of the results already obtained, the bacillus cul- tivated on bile is worthy of attention because of its harmlessness for bovines and the slowness of its resorption. Furthermore, it is harmless to man. We may therefore admit that it is worth using for the vaccination of bovines, without danger, for con- ferring on them, if not immunity, the resistance of which re- search in antituberculosis vaccination has revealed the mechan- ism and demonstrated the possible realization.’’ Recently-qualified and somewhat haughty graduate: ‘‘Oh, yes, my practice has increased 100 per cent since Christmas.”’ Candid acquaintance: ‘‘Somebody brought in another dog, then ?’’—The Veterinary Record. 1 Vol. 11 (n. s.), p. 599, Feb., 1921; vol. 12, p. 274. June, 1921. 2Vol. 30, p. 3138, June 15, 1921. CLINICAL AIDS IN THE DIFFERENTIAL DIAGNOSIS OF THE COMMON POULTRY DISEASES ' By F. R. BeaupetrtTe Manhattan, Kansas THIS PAPER is written with the view of aiding the general practitioner in the differential diagnosis of the more common poultry diseases and not as a guide for laboratory diagnosis. The diseases discussed will be those peculiar to this section of the country. It is realized that the practitioner’s diagnosis in most cases must be made in the field without laboratory facilities and that his judgment is based principally upon the history, symptoms and postmortem findings; therefore these points will be discussed rather than a detailed description of the bacterio- logical findings in the case. In the septicemias a differentiation is very often impossible and must be determined by a bacterio- logical examination. Such a differentiation is often not neces- sary, since the control measures for one will suffice for the other. In those cases where vaccine therapy is to be used, an accurate diagnosis is, of course, necessary, and here the laboratory may be called upon for confirmation. An analysis of the records shows that roup is the most wide- spread and most frequent disease of poultry with which we have to contend. Contrary to most opinions, this condition is found to occur throughout the year, and not in winter, as is commonly supposed. The ravages of cholera are fohnd to de- crease somewhat during the winter months, while fowl typhoid seems to be more prevalent in spring and early summer. Black- head appears to be most common in the month of October, and tuberculosis has no seasonal variations. White diarrhea is, of course, a seasonal disease, occurring during the hatching months of March, April and May. This year botulism has been encoun- tered very often, especially after heavy rains. This paper will, therefore, necessitate a discussion of the above-mentioned diseases, together with those rare maladies from which they are to be differentiated. 1 Presented at the fifty-eighth annual meeting of the American Veterinary Medical Association, Denver, Colo., September 5-9, 1921. 283 284 F. R. BEAUDETTE Fow. CHOLERA Fowl cholera is an acute infectious disease of a septicemic nature affecting fowls, geese, ducks, turkeys and pigeons. It is characterized by a continuous high fever, diarrhea and gen- eral depression. The mortality may run as high as 90 per cent. The course of the disease is rapid, death often occurring in 24 hours after symptoms have developed. One usually gets the history that dead birds were found under the roots or on the nests. In very acute cases prodromal symptoms are often ab- sent. In chronic cases the birds may linger for several weeks and in some cases recover. Affected birds are greatly depressed ; the head is held close to the body, the feathers are ruffled, the comb and wattles are usually cyanotic, and a diarrhea is always present. The color of the droppings is usually yellow, though they may be of a greenish tinge or even streaked with blood. The bird refuses food and in the later stages of fatal cases water is also refused. The eyes are kept closed in the later stages of the disease, and a slime collects in the mouth. In chronic cases emaciation is quite marked. On opening the careass of a bird dead of cholera the typical picture of a septicemic disease is found. The blood vessels of the intestines are highly congested. Hemorrhages are found throughout the intestines, though more particularly in the first and second portions of the duodenum, and in the rectum, and less frequently in the ceca. The liver is congested and friable. The kidneys are marked with white lines indicating the portion of the tubules distended with urates. The ureters are likewise filled with the same material. The spleen is congested and fri- able. The pericardial sac is partially filled with a straw-colored fluid which may be fibrinous, and the surface of the heart shows petechial or ecchymotic hemorrhages, particularly along the course of the coronary vessels. The lungs are usually pneu- monic. oo ee a Cholera is to be differentiated from fowl typhoid, which at times is very difficult. In fowl typhoid the same symptoms may be present with the exception of a profuse diarrhea. If diarrhea is present in the latter condition the droppings are more green- ish in appearance. In fowl typhoid the comb and wattles are rarely cyanotic, though they may show congestion; usually they are anemic. The intestines contain a more solid mass and ap- pear anemic. The duodenum may contain a quantity of bile DIFFERENTIAL DIAGNOSIS OF PoULTRY DISEASES 285 giving it a greenish tinge. White spots on the surface of the liver and heart in the case of fowl typhoid will aid in the dif- ferentiation. The temperature and color of the comb will dif- ferentiate cholera from botulism. Fow. TyPHoIp Fowl typhoid is also a specific infectious disease of the fowl, and at present is more prevalent in this section than cholera. As previously stated, the comb and wattles are usually dry and anemie. The mucous membranes of the head are pale and the bird is depressed. Older stock is. more often affected than young. Unlike cholera, the course may extend over a week or so, except in chronic cases, when it is much longer. The mor- tality is at times as high as in cholera. The autopsy shows an anemic condition of the intestines, though there may be petechial hemorrhages in the first and sec- ond portions of the duodenum. They contain little feces, and this is usually stained with bile. The liver shows on its surface white spots about the size of a pin-head and the spleen is con- gested and softened. The kidneys are usually of a clay color; at other times congestion is outstanding. Distention of the gall bladder is almost a constant finding. The heart shows white spots similar to those of the liver. The lungs are normal in contrast to the pneumonic condition in cholera. Rover Roup is an infectious disease which may manifest itself in several ways. The condition is variably termed contagious catarrh, coryza, cold, pox, diphtheria, swell head, contagious epithelioma, etc., depending on the location of the lesions. One or more forms may be present in the same individual. The mortality of roup is from 5 to 80 per cent, and the course varies with the severity of the disease and parts affected. In the cutaneous form recovery may take place in a week or so and the mortality is very low, while in the diphtheritic and oc- ular forms the course is much longer and the mortality is usually high. In all cases the egg production is decreased. It is a disease of the mucous membranes of the head and the un- feathered portions of the body. Birds of all ages are affected except chicks a few days old. When the nasal cavity is involved there is at first a dirty, foul-smelling discharge; later this be- 286 F. R. BEAUDETTE comes thicker and finally causes the stoppage of the cavity. The mass may enlarge and cause a bulging in the mouth. The bird is compelled to breathe through the mouth, and this may cause a drying of the tongue, often called pip, which may result from any condition that will cause a drying of the tongue, usually associated with oral breathing. The mouth is the location of the diphtheritic form, and here patches of false membrane are found, which vary in size from a pin-head to several millimeters in diameter. At first they are white; later they turn gray or yellowish brown. They may be removed from their attachment, and an easily bleeding raw sur- face is left, from which another membrane may develop. These patches may be located anywhere in the mouth, and may extend down the trachea to the bronchi or down the esophagus to the crop. When lesions are located in the larynx the gravity of the condition is increased. In the ocular type a watery discharge from the eye is first noticed. This becomes thicker, and the frequent closing of the eye causes the material to become frothy. Finally the eyelids are glued together and the mass collects beneath. If the pres- sure is not released, destruction of the eye will result. If the lids are separated a yellow, cheesy mass protrudes. = Normal Abnormal Total solids, dried at 105° C 2.38 pee? Anamo ae cone Aiea taro a sere 0.7 - Lotalinitrogeni(N)) 2... s45-2+22- oe ees 48 1.70 49 Many more horses would be in use today if more agricultural and weekly papers would push forward the facts. Certainly the tractor people are not going to do it. An unfortunate fac- tor in the situation is the attempt by certain big car producers to make agents take a quota of tractors in order to get cars. Such coercion is wrong. The automobile has come to stay, but horses are no more evanescent than bicyeles, of which latter vehicles more were sold in 1920 than ever before. Our advice to farmers is: Breed good work horses; they will sell for double present prices some day. To manufacturers and merchants we say: If you are using horses now, keep them; if you are using trucks, get some comparative cost figures on horses vs. trucks. They will surprise you—United Business Service. » FIELD OBSERVATIONS IN LOCO POISONING 1 By O. E. Troy Raton, New Mexico IN the preparation of a paper on this subject I make no claim of advanced knowledge, but I have chosen the subject because it is one regarding which we have accomplished nothing and I feel it a duty to bring the matter before the veterinary profession with an appeal that it receive more careful study, commensurate with the enormous loss sustained by the livestock industry over the entire Rocky Mountain slopes. For the past seven years I have been in constant association with the condition commonly known as ‘‘loco,’’ both from a professional standpoint and from that of a livestock producer, and I assure you that there is no pathologic condition with which I have ever contended that presents a more difficult as- pect. As to its economic importance I venture to state that in districts infested with the white loco (Oxytropis lambertz) the loss far exceeds all other losses combined, and its toll is not con- fined to actual deaths or depreciation in value of livestock, but it constitutes an absolute bar against the importation of high- grade or purebred livestock. Native stock, especially of infe- rior quality, does not readily eat the loco weed, except under extreme circumstances, but the higher type of imported stock can not be grazed on loco-infested ranges with impunity. For the consideration of this paper I confine myself to the white loco (Oxytropis lamberti). The purple or woolly loco (Astragalus mollissimus) is credited with being injurious, but is of relatively little importance. The symptoms of loco poison- ing will become manifest in sheep between thirty and sixty days after they begin eating the weed and will he first noted by the herder when the individual separates from the herd, always secking the rocky knolls in search of loco weed, the head up, nose extended, unmindful of the voice which it normally obeys. The gait becomes stiff, with marked lack of coordination. The eye develops a wild, staring glare. If continued grazing on loco pasture the animal soon refuses all other feed, strays from the herd and becomes food for coyotes or dies of malnutrition. 1 Presented at the fifty-eighth annual meeting of the American Veterinary Medical Association, Denver, Colo., September 5-9, 1921. 2 299 300 O. EK. Troy The lesions found in animals suffering from loco poisoning are never such as could identify the disease. I will give here the complete autopsy on a sheep which showed symptoms of loco poisoning in February, 1921. As soon as symptoms de- veloped it was removed from loco-infested pasture and kept in a small pasture until August 28, at which time it was destroyed for the purpose of autopsy. The animal showed typical symp- toms of loco poisoning. The subcutaneous tissues were normal but entirely devoid of adipose tissues. The contents of the pleural cavities were normal in every respect. Those of the peritoneal were likewise normal. Careful search was made through the digestive tract for ulcers, which are reported to be lesions found indicating loco poisoning, but no ulcers could be located. General lack of adipose tissues was noted, although the animal had been on the best of pasture. The head was sawed through the median line so as to expose the brain tissue, which seemed normal in color and consistency, although the meningeal vessels indicated hyperemia. The spinal column was cut into sections, then severed lengthwise so as to expose the spinal cord throughout, which also seemed normal in color and consistency, though again I noted hyperemia. Other than this hyperemia I could note no lesions. The symptoms of loco poisoning in cattle are more varied than in sheep. In fact, I have studied three distinct types of reaction to the loco poisoning. The symptoms apparently de- velop less rapidly than in sheep, but this may be due to the fact that they are not under constant observation. However, sixty days after cattle are noticed to prefer hilltops or rocky mesa sides on which the loco weed abounds will suffice to de- velop well-marked symptoms. They become gaunt from lack of water, as they will stay in a loco patch for days without drink. Constipation is marked, the gait stiff, with lack of coordination, and there is a wild, staring eye. They are intensely nervous and if startled by a sudden shout they tremble in a way painful to see. Many cases progress until they lose the senses of sight and hearing. JI have watched advanced cases in a recumbent position that seemed to have hallucinations; will prehend and masticate imaginary food and will be entirely unaware of one’s approach until touched. The second train of symptoms are manifestly different. The drug must concentrate its effect upon the vasomotor instead of FIELD OBSERVATIONS IN Loco POISONING 301 motor nerves or ganglia, while the stiffness and lack of coordi- nation are present in a milder degree, and instead of the tucked- up, gaunt appearance a distention of the abdomen is apparent, due to abdominal dropsy, which progresses until the animal be- comes helpless and dies of suffocation or from a ruptured dia- phragm. These cases can not be cured, but the simple operation of tapping, a trocar inserted through the linea alba about six inehes anterior to the udder, will draw off the peritoneal fluid and give immediate relief. I have drained as much as eight gallons of fluid in this manner with excellent results, excepting in pregnant animals, where abortion invariably follows. These cases, if kept off the loco weed and given a ration of cottonseed cake, will make, to the casual observer, a complete recovery and the succeeding season will fatten remarkably. Regarding the third train of symptoms, I feel that I am treading on ‘‘thin ice.’’ I make my statements guardedly, with the hope that they will bring out full discussion and possibly lead to further study and investigation that will throw greater light on the subject. We have in the higher altitudes of New Mexico and Colorado a disease known as brisket disease, which seldom appears at an altitude less than 8,000 feet. The symp- ~ toms of this disease have a striking resemblance to those de- seribed above, except that the serum collects in the pleural ecav- ity and pericardial sacs instead of in the peritoneal cavity. This disease appears with greatest frequency in animals taken into the mountains from lower altitudes and, in two cases which have come under my observation, from loco-infested ranges. Dr. E. P. Johnson, of the New Mexico State College, has dem- onstrated that acute cases of this disease can be controlled by a judicious use of digitalin and if moved to a lower altitude will fatten and make marketable beef, although, like all other cases of loco poisoning, they never regain a normal, robust constitu- tion. The effect of loco poisoning on thé horse is not of such great economic importance as it was formerly, owing to the rapid con- version of western ranges into farms and the fact that the horse’s ration is more easily controlled, yet the loss from loco poisoning in the horse is enormous. Like the case of other species, the imported horse is more susceptible, but it is difficult to raise horses on loco-infested ranges without heavy loss. The loco-tainted colt is abnormally wild, seldom learns to lead, and 302 O. E. Troy will never become reliable. If it continues to feed on loco weed the gait becomes stiff and faulty coordination develops. The sensitive nervous system becomes supersensitive, while the motor centers become slow to respond. The senses of hearing and sight are impaired and ultimately total blindness develops, de- elutition becomes difficult and impossible. On the range the animal usually dies after falling between the rocks, in a ditch, or in shallow water where it drowns. If kept off the weed from early stages horses can be kept serviceable but never reliable. The effect of advanced loco poisoning upon goats is one of the most pitiable sights I ever witnessed. Possibly because of their remarkable vitality they endure greater punishment and will follow the bunch when their front or hind quarters are completely paralyzed, dragging themselves in a sitting posture or walking apparently on the hind legs or head. One of the great sources of loss from mild loco poisoning among cattle and sheep is that of abortion, and I deem it wise to caution veterinarians, and especially producers of contagious abortion vaccine, to beware of its use where loco poisoning ex- ists, for it will not give desired results. There is an opinion extant among investigators of loco potl- soning that the condition does not consist of poisoning, but is rather a deficiency disease, or, as one investigator stated to me, “Tt is a condition which can be produced by feeding sawdust.’’ I cannot agree with this conclusion. I am convinced that the weed contains a drug, a craving for which keeps the animal in search of it. I have driven animals off of loco pastures and en- deavored to confine them in loco-free pastures, and I find that we can not build wire fences which are as strong as their desire for the weed, and we have had to resort to a corral in all ad- vanced cases. Many ranges have been abandoned entirely on account of loco. Other persons have attempted to rid their ranges of the weed by digging, but the cost of this method, in my experience, is prohibitive. At present our only relief is obtained by range rotation. My observation convinces me that the loco weed develops its poisonous properties in greater quantities on high, rocky soils, mesa sides, ete., and I find that by using these soils when forage is abundant we can minimize our loss from loco. Toward the late winter and spring we endeavor to keep stock on the lower lands and bottoms. In partial support of my views on this subject I will quote the conclusions reached = FIELD OBSERVATIONS IN Loco POISONING 303 by Dr. Law in his article on loco poisoning in ‘‘ Veterinary Med- icine,’’ vol. 8: 1. There is some poison in loco weed which may cause the illness and if sufficient quantity is taken the death of an animal. 2. This poison is contained in the decoction obtained from the plants, and by systematically feeding it to healthy cats, cases of loco disease may be produced. 3. Taste for green loco weed may be experimentally produced in the jack rabbit. 4. From the large quantity of the plant or the decoction required to produce the disease, the poison must be weak; or if strong, it must be in a very small amount. Since this article was published in 1905 the Department of Agriculture, in cooperation with the State of Colorado, con- ducted an extensive four-year experiment at Hugo, Colorado, the results of which were published in Bulletin 129 of the Bu- reau of Plant Industry, Department of Agriculture, in 1908. This bulletin is intensely interesting. It is the result of long, painstaking study, but the conclusions, which I will quote in part, have not been taken seriously by the profession, and if correct, have not been followed up by any logical method of cure or control which would make it of economic value. 1 quote in part from the introductory statement: This discovery also seems likely to provide a basis for rational treatment of locoed stock. Unfortunately, the discovery of the fact that barium is the poisonous constituent of loco weeds came too late to aid in the search for remedial measures on the range during the period covered by this report, but those empirically arrived at have received additional support from these laboratory results. Thus the work in the field and undertaken after repeated attempts and dis- couraging failures by others has yielded results to persistent scien- tific research and promises practical aid to the now suffering live- stock interests. The livestock industry is still suffering. The above promise is as yet a promise. The conclusions in Bulletin 129 I will quote in part: 1. Conditions analogous to those met with in locoed animals occur in other portions of the world, especially Australia. 2. The main symptoms described in stock on the range can be reproduced in rabbits by feeding extracts of certain loco plants. Those especially referred to here under the term of loco plants are Astragalus mollissimus and Aragallus lamberti.1 8. The production of chronic symptoms in rabbits is a crucial test of the pharmacological activity of these plants. 4. The inorganic constituents, especially barium, are responsible for this action, at least in plants collected at Hugo, Colorado. Per- haps in other portions of the country other poisonous principles may be found. 5. A close analogy exists between the chemical symptoms and 1Oxytropis lamberti. 304. O. E. Troy pathological findings in barium poisoning and those resulting from feeding extracts of certain loco plants. Small doses of barium salts may be administered to rabbits without apparent effect, but suddenly acute symptoms set in analogous to what is reported on the range. 6. The administration of sulphates, especially Epsom salts, to form insoluble barium sulphate, would be the chemical antidote which would be logically inferred from the laboratory work, but of neces- sity would have to be frequently administered, and its value after histological changes in the organs have occurred remains to be set- tled. At present it seems best to rely upon preventive measures rather than antidotal treatment. 7. Loco plants grown in certain soils are inactive pharmacologi- cally and contain no barium. In drying certain loco plants the barium apparently is rendered insoluble so that it is not extracted by water but can easily be extracted by digestion with digestive ferments. 8. The barium to be harmful must be in such form as to be dis- solved out by the digestion. 9. In deciding whether plants are poisonous it is desirable not merely to test aqueous or alcoholic extracts, but also the extracts obtained by digesting these plants with the ferments which occur in the gastro-intestinal tract. It will be noted in paragraph 7 that these investigators have found that in some soils the loco weed does not contain a poison- our property. This conforms with my observation and is the basis of the plan we now follow to avoid loss from loco. How- ever, nothing of value has thus far been developed from these investigations to afford the promised relief to the livestock indus- try. The investigators above referred to conclude that ‘‘we must rely upon preventive measures.’*! With this I heartily agree. I will, therefore, briefly review the history of loco poi- soning from the observations of stockmen and present a plan that may prove a feasible method of prevention. The loco weed is infested in some localities with a parasite, a larval form of fly, which the early observers believed to be the cause of the poisoning, arguing that the parasite was eaten with the weed and contained the poison. I have been informed by three observers in widely separate areas that the infestation of this parasite destroys the loco weed. One observer who made an effort to destroy the loco weed in an 8,000-acre pasture by dig- ging informed me that he found this parasite present on a part of his range. He purposely left an area of 10 acres to ascertain the result. This plot was marked by stakes and an estimate made of the number of loco plants. He informs me that afte: the second year the loco weed had entirely disappeared and that the area is still free. According to the best information I am able to collect, this fly deposits its eggs in the base of the loco 1A later publication of the Department of Agriculture, Farmers’ Bulletin 1054, discusses preventive measures.—EDITOoR. FreLD OBSERVATIONS IN Loco POISONING 305 plant, the larva burrows into the plant and destroys the root. I was unable in the limited time at my disposal to obtain speci- mens of either the fly or the larva to present here, but I believe the idea worthy of further study, for it may develop a feasible method of prevention for loco poisoning. The digging of the loco weed is our only alternative! If this method is adopted it must be carried out for at least three, and better, five years. I have figures which indicate this can be done at an expense of 25 cents an acre per annum, but my experience differs. There is on the market a chemical known as ‘‘Loeco Lion’’ which is sold to destroy loco weed. This product must be injected into the base of each plant, and, according to one experiment upon which I have data, it is very efficient, at a cost of 40 cents an acre and labor. To be ultimately success- ful the problem of eradicating loco weed must be coordinated by the Government on a plan similar to that of the Biologic Survey in its rodent extermination campaign, in order to clear the range over large areas and prevent reseeding. In conclusion, I respectfully recommend that this matter be taken up by the proper committee to put the available data in concrete form and present at the next meeting recommendations for, the approval of this Association, with the view of requesting some line of action of the United States Department of Agricul- ture which will provide relief to the suffering livestock industry. GEORGE M. ROMMEL RESIGNS George M. Rommel, chief of the Animal Husbandry Division of the Bureau of Animal Industry, who has been with the United States Department of Agriculture for 20 years, has re- signed to become editor of an agricultural magazine. Mr. Rommel built up the Animal Husbandry Division and had done distinguished and effective work that is recognized throughout the country as well as in the department. A native of Iowa, he is a graduate of the agricultural school of the Iowa State College at Ames. He is the author of various bullétins and articles on animal husbandry topies, a fellow of the Ameri- ean Association for the Advancement of Science, and secretary of the American Genetie Association. 1 Farmers’ Bulletin 1054 recommends severing the root below the crown of buds and states that it is not necessary to dig out the whole root.—Eprror. THE NEED OF COOPERATION BETWEEN THE UNITED STATES AND MEXICO FOR THE CONTROL OF EPIZOOTICS 1 By L. Santa Maria Veterinary Inspector, Department of Public Health of the Republic of Mexico, Piedras Negras, Mexico IT IS with great difficulty that I undertake to prepare a paper in the English language, as I lack experience in its use; but I undertake to do so feeling sure that you will pardon mis- takes which are unavoidable. Before entering into my subject I will take this opportunity to assure you that I bring the most cordial greetings from the veterinarians of Mexico to the members of this Association. The veterinarians of Mexico are fully aware of the great advances made in veterinary science by the members of the profession in the United States. We have watched them in their successful efforts in the control of infectious diseases of animals; we are acquainted with the wisdom of your laws relating to the move- ment of livestock, the regulations governing the inspection of meat, milk and other articles of food, the organization of your Army veterinary service, your enormous progress in biologic therapy, ete. The names of the leading veterinarians in this country are familiar to us, and we have obtained and used to our great advantage their books and articles. Cordial relations between the veterinarians of our countries have never been initiated, on account of the revolutionary con- ditions which have kept our country in a turmoil of successive bad governments, which have prevented all constructive prog. ress. By good fortune, Mexico has at present a strong, honor- able government, in the leadership of which we have General Obregon, a man of energy, honor and good intentions, who has’ been able to establish peace and to give new life to the wheels of industry by providing full protection of life and capital to both the native and the foreigner. — As a eitizen of Mexico, a country desiring the friendship of the United States, in spite of the statements to the contrary by 1Presented at the fifty-eighth annual meeting of the American Veterinary Medical Association, Denver, Colo., September 5-9, 1921. 306 ia COOPERATION BETWEEN UNITED STATES AND Mexico 307 certain sections of the press of this country; as a veterinarian of Mexico, and as a representative of the Department of Public Health of Mexico, I wish to express my best wishes for the prog- ress of this Association, and urge a more sympathetic under- standing on which to build for our mutual benefit. It is a well known fact that the transportation of livestock from one locality to another, or some of their products like hides, horse hair, bones, ete., constitutes one of the most frequent and dangerous causes of epizootic disease propagation in live- stock. There is not one veterinarian that has not had the oppor. tunity of seeing some cases of infectious and contagious dis- eases which have developed in some locality coinciding with the arrival of livestock from other localities. Epizootic diseases are of daily occurrence in livestock shows, fairs and expositions. In this way we could cite a multitude of examples to demonstrate the truth of what I have said. The sanitary authorities of the civilized countries have al- ways understood these facts clearly, and to that effect they have dictated a series of laws tending to regulate the transportation of livestock, thereby eliminating a serious danger. The sanitary departments of the different States of the Union of America have in this respect a great many sanitary laws which, without doubt, are the most complete of their kind. I wish to express my admiration for such laws, of which the United States should be proud. Contrary to what is supposed, the international interchange of livestock between the United States and Mexico has not been given its due importance from the sanitary point of view. The geographical location of the two countries makes possible be- tween them a development of an active commercial interchange of all kinds of articles, livestock not excepted. Such interchange will be increased as a natural result of greater activities; there- fore this is a good opportunity to point out the necessity of initiating measures to make more efficient the sanitary inspec- tion of animals and their products not elaborated in the border of the United States and Mexico, an inspection which up to ’ date has not been as strict as it should be. For example, I have seen hundreds of freight cars, loaded with hides, crossing the boundary to be interned in the United States, the origin of which nobody knew, and that perhaps came from regions infected by anthrax or by some other contagious 308 L. SANTA Maria disease, without any more requirement than a statement from the merchant before the American consul that the hides came from regions which were not infected by any infectious or con- tagious disease. It is easy to understand that such a require- ment is useless, because in the majority of cases the interested party in this line of business has not the least idea of what he is stating, doing it only to obtain the permit he is after, with- out knowing the great danger such a statement engenders in this class of affairs. In such case the veterinary inspectors of the Mexican Government could have given good service by demand- ing information in detail, and when that was not obtainable, by arranging under their vigilance the disinfection of the hides. Never have they been asked for their cooperation in such a way. In the same way I have known of animals that have been de- clared tuberculous in the United States and have been sent into Mexico by some unscrupulous ‘individuals taking advantage of the fact that some places on the Mexican border are in need of veterinary inspectors, or defying criminally the vigilance of the authorities. In cases like this the American authorities would have helped greatly by prohibiting the exportation of such animals. To the high criterion of this assembly I have undoubtedly referred sufficient cases to understand the imperious necessity of correcting such deficiencies, which can be brought about only by an understanding between the sanitary authorities of the United States and Mexico. The understanding, if realized, would be beneficial. It would eliminate such dangerous causes of contagious disease propagation, and greatly facilitate its control. H It would be very difficult for me to submit to you any set of rules, which necessarily will have to be formed if an under- standing is to be reached between the two countries. Neither do I consider this an opportune time to discuss such a docu- ment. I wish, nevertheless, to point out very briefly a few things which, owing to my experience as veterinary inspector on the border, I think should be taken up first. It would first of all be indispensable to establish that every person or corporation desiring to export livestock or their products from Mexico or the United States should be required to secure a permit or health certificate from the sanitary au- thorities. It would then be possible to prevent the transpor- am Ge Ny A Sah SON ED § were fire oe i AN hn CooPERATION BETWEEN UNITED STATES AND Mexico 309 tation of livestock or their products from districts in which in- fectious diseases exist. It would be possible to impose a mul- titude of highly beneficial measures, such as prohibiting all classes of commercial interchange of animals that have been de- elared tuberculous; to require that all bovine animals intended for breeding when proceeding from a tick-infested zone, should be dipped previously in an arsenical solution; to arrange that hogs before being transported should be vaccinated against hog cholera, and other things that will contribute to lessening the danger, from the hygienic point of view, of the transporta- tion of animals. : To my knowledge, the carrying out of such measures as I have briefly mentioned will result beneficially to the livestock industry and to those who are interested in it. When the in- terested parties in this line of business of Mexico, as well as those of the United States, are convinced that the interchange of animals or their products does not offer any danger from the sanitary point of view, because it is subjected to a rigid scien- tific examination, they will increase it. It would be useless to try to demonstrate all the advantages that will come with this intensification of commercial activities. It presents to the doc- tors of veterinary medicine a new opportunity to realize a work of positive utility. I am sure that. I have made myself under- stood, although my paper is a little confusing, and if my propositions are taken into consideration and practiced we will have contributed to the already existing idea in society that the mission that has been recommended to us professionally is transcendental. It may be of, further interest to this Association to know that during the months of May, June and July, 1921, approxi- mately 38,000 head of cattle, goats, sheep and hogs crossed the international boundary at Eagle Pass, Texas, into Mexico, this being the third in size of entries into Mexico from the United States. ~ As a heritage of the war, dourine has appeared in Belgium and in the northeastern part of France, where it is said to have been introduced by German army horses. BSS — be, We a Ee e wee Sst Se a = a et Sheep Affected with Stomach Worms CLINICAL AND CASE REPORTS 327 attached to the stomach walls. They were also found in a simi- lar way in the duodenum throughout half its length. The female is about an inch in length and the male one-half to two- thirds of an inch. They agree with the description given by Dr. B. H. Ransom for the stomach worm of sheep (Haemonchus contortus) in the Cornell Veterinarian of April, 1920. Treatment—The 1 per cent copper sulphate aqueous solu- tion, as first used successfully by Thieler in South Africa and recommended by Ransom, was tried. The following doses were used: For lambs 3 months to 9 months old, 20 to 50 mils; sheep over 1 year of age, 60 to 100 mils. All animals treated were fasted from 18 to 24 hours before they were given the treatment. The treatment was repeated twice at an interval of three weeks. Four flocks of sheep were treated, with a total of 2,000 sheep. In three of the flocks the results were excellent, the disease cleared up, the animals gained weight rapidly, and in some of the advanced cases the edema of the jaw passed away. The fourth flock treated was located on an adjoining sabana about 60 miles away, and in spite of the treatment the sheep kept on dying at the same rate and showed the same symptoms after treatment as before treatment. An effort was made to find out the reason for the failure of the treatment by looking for the eneysted stomach worm, Strongylus (Ostertagia) ostertagt. On autopsy the lining of the fourth stomach (aboma- sum) was found to be studded with raised white swellings about the size of a pinhead to a small pea; the wall of the stomach was twice as thick as normally and a straw-colored exudate was present in the walls and in the abdomen. The encysted stomach -worm was found in the raised swellings. Strongylus (Haemon- chus) contortus also present in large numbers. It was recom- mended that the visibly affected sheep be slaughtered and that the pasture be changed to a dryer region. This is being carried out, and it is too early as yet to make-any statement as to the result. Some bladder worms, Monieza alba, were found in a few im- ported sheep which had died from other causes (in transit). No cases of @sophagostoma columbianum (nodular disease) in the intestines were found. The only external parasite found was the common sheep tick. 328 CLINICAL AND CASE REPORTS GRANULOMA OF THE FROG IN THE HORSE By Ernest F, JARDINE Government Veterinary Surgeon, St. Kitts, British West Indies THERE is a condition here, seen most frequently in mules, but occasionally in horses, in which the horn of the frog disappears entirely leaving bare the sensitive structures, which naturally, from constant friction with the earth, irritates the parts causing exuberant granulations accompanied with lameness. On the other hand, and these are the most frequent, from the excessive height of the wall, the hoof becomes contracted; but this con- traction is seldom accompanied with lameness, and but for the condition of the frog, which becomes injured from sharp stones on the road, the animals could continue at work. The condition has a certain resemblance to canker, with the exception that it has no tendency to involve or encroach upon the sole; and the rapidity with which the condition responds to treatment. JI am frequently puzzled as to how and why it is that even the con- tour of the frog disappears and leaves a denuded, bleeding sur- face, sometimes the shape of a marble with a pedicle, and at other times with a sessile base. I attribute the cause to filth, as it is rarely seen in well kept animals. The continual standing in urine, the ammonia of which macerates and decomposes the horn, will produce it. Treatment: If .he condition is seen in the early stages, it is comparatively simple to treat. Lower the wall, dust parts with calomel and iodoform, alum, copper sulphate in powder, or 50 per cent solution; pine tar on oakum with leather sole to retain in place; all have their sphere of usefulness. In fact, the condi- tion is treated as one would a case of thrush, which is generally the origin of it. When the condition becomes neglected and long standing, when it becomes a sort of tumor, the only cure is extirpation. If the growth is small, with a well-defined neck and the ani- mal is of a quiet disposition, I usually hook the growth with a tenaculum and with a quick incision excise it; otherwise, the patient is cast, and the growth dissected out. The wound is covered with boric acid packed with cotton wool and bandaged. The dressing is removed in 24 hours to 48 hours and if there is no formation of pus, it is not washed; otherwise it is, and redressed with borie acid. . In about one week’s time CLINICAL AND CASE REPORTS 329 the animal is shod, the part protected with tar and oakum and a leather sole, and the animal returned to work. Of course it is understood that everything connected with the operation is done as aseptically as possible. INTUSSUSCEPTION IN A COLT By C. A. Cary Auburn, Ala. At the Veterinary College clinic of the Alabama Polytechnic Institute, Dr. I. S. MeAdory brought in a colt in a moribund condition. This colt was about one week old. No definite diag- nosis was made and the colt soon died. Post-mortem examina- . Poa peeiay at Gta Nat G> Peete Intussusception in a Colt tion was made by Dr. N. G. Covington and eight intussuscep- tions were present in the small intestines. Two of them were displaced by students examining them. The other six are shown in the accompanying photograph. A lady student of the University of Liverpool, England, Miss Edith Gertrude Knight, has been successful in passing with honors the second examination for the degree of Bachelor of Veterinary Science and also the second examination for the diploma of M. R. C. V. S. ABSTRACTS A StmwpLe METHOD FOR THE DETERMINATION OF THE NUMBER OF BACTERIA IN BACTERIAL SUSPENSIONS. K. A. Fries. Cen- tralbl. f. Bakt., Jena, 1921, vol. 86, pp. 90-96. After reviewing the various methods used for the standardi- zation of bacterial suspensions, the author is of the opinion that the standardization by the counting chamber method has proved most satisfactory, but based upon his own work and also upon Saltmann’s extensive investigations carried on in the Hygienic Institute of the University of Berlin, he is of the opinion that if it is desired to count small bacteria, the counting chamber method is very tedious and tiresome for the eyes; besides it also consumes a great deal of time. In the experience of the author the following method which he developed has proved very rapid and most accurate for the standardization of bacterial sus- pensions. PREPARATION OF THE STANDARD FLUID A suspension of yeast in physiological salt solution preserved with 5 per cent phenol is prepared. The strong preservation is necessary on account of the difficulty of keeping the standard fluid in a sterile condition. Other methods of preservation might give equally as good results. A piece of ordinary yeast (Saccharomyces cerivisiae) is dis- solved in the phenolized salt solution. The yeast should be fresh, otherwise there is danger that foreign bacteria may be contained in the same and frequently in such great quantities that their presence may later be detrimental for the determina- tion of the number of bacteria in the suspension. The number of yeast cells in the standard fluid should vary between 20 and 30 million per ce. In order to obtain a sus- pension of this strength it is as a rule sufficient to dissolve a piece of yeast the size of a hazel nut in one liter of carbolized salt solution. The flasks should also contain approximately 100 small glass beads. Following the dissolving of the yeast the bottle should be shaken preferably in a shaking apparatus. The shaking should distribute the yeast cells uniformly throughout the fluid. The counting of the yeast cells in the fluid is then undertaken. 330 ow —— ork +4 Wade< 3 >) oot" het eee a ABSTRACTS 331 For this purpose a Thoma-Zeiss or the Hayem-Nachetsche cham- ber is preferable. A control counting in both of the chambers is desirable. The results will be more satisfactory if the great- est number of squares are counted in the chamber. In the Thoma-Zeiss apparatus at least 100 squares should be counted; whereas in the other 8 to 10 squares will be sufficient. If the count shows that the number of cells per ce. is of the desired quantity we have a suitable standard fluid. One liter of such fluid is sufficient for about 500 determinations. Should counting of the standard fluid show a greater num- ber of yeast cells than 20 to 30 million per cc., the fluid should be diluted to the desired quantity; whereas if the number of cells are not present in the required numbers, then either more yeast is added or the suspension is allowed to stand for several days, after which some of the supernatent fluid is decanted, which naturally would increase the number of cells per cc. in the remaining fluid. On account of the large size of the cells the counting is readily accomplished, which also facilitates a comparative counting of the bacteria in the suspension to be counted. THE METHOD OF PROCEDURE OF THE BACTERIAL COUNTING With the aid of the standard fiuid the bacterial contents of any suspension can be readily determined. After thoroughly shaking, 0.2 of a ce. is taken from the fluid to be tested with the aid of a pipette and placed into a flask or a reagent glass. Then 2 ec. of the well-shaken standard fiuid is added to the bacterial suspension as soon as possible. The mixture is then thoroughly shaken, and with the aid of a Pasteur pipette a small quantity is taken up-and quickly dropped upon a clean slide. This is carefully dried over a flame or in the air and the preparation is then carefully stained. Any ordinary stains are satisfactory for this purpose. However, a diluted fuchsin stain is prefer- able. As a result of the phenol conténts in the standard fluid a very good staining is obtained with the fuchsin. After the stain has acted upon the preparation for a short time it is rinsed off and examined under an oil immersion lens. If it is found that the cells are well distributed, as is usually the case if proper care has been taken, the counting may be under- taken. On the other hand, if it is found that the cells are not well distributed, in order to count them aceurately the mixture bo ABSTRACTS CO eX) is diluted with the corresponding quantity of physiological salt solution before the counting slide is prepared. It may also occur that _the relation of the yeast cells to the other cells is not such that an accurate counting may be undertaken as, for instance, when the proportion of the bacteria is very much larger than the number of yeast cells. In such cases, of course, this relation may be changed by another mixing, for instance, using a smaller quantity of the bacterial suspension to be examined. Even the beginner will soon find the correct way as to the best procedure. It may be accepted as a rule that by counting with the so-called net-micrometer, the number of organisms can be counted in even a more concentrated form than if the counting is made in consecutive fields. Therefore a rule may be estab- lished that for accuracy’s sake not less than 250 yeast cells and the corresponding number of bacteria should be counted. Of this number, 250 yeast cells may be found either in 10 different fields or in a greater or smaller number of squares in the net- micrometer, if such is used. If we assume that in counting 10 fields we find 256 yeast cells and 814 bacteria and if of the bacterial suspension 0.2 ee. and of the standard fluid 2 ee. were used the question arises, How many bacteria are present per ce. in the suspension? The desired number is then found according to the following formula: Volume of Yeast Cells. — Number of Bacteria. K — X Volume of Bacteria. Number of Yeast Cells. K is the consonant which indicates how many millions of yeast cells are present per ce. in the standard fluid. Thus, if the above figures are placed in this formula and if the contents of the standard fluid is taken as 25 millions per cc. then we have: 2 814 Se a 9 eee (795) =—800 0,2 256 The number of bacteria in the bacterial suspension therefore was 800 million per cc. In case the first examination of the slides establishes a mixture of too large a concentration in order to make an accurate counting possible, then the mixture can be diluted as stated above. This, however, has no effect what- soever on the above formula. The preparation of the standard fluid for counting should proceed rapidly. With this in view immediately after placing av hwia es ~s ” gil eae i ha ABSTRACTS 333 the small drop of the suspension on the center of the counting chamber the cover glass should be slid from the side over the drop. If this operation is not rapidly carried out there is dan- ger that the counts may be erroneous. The best results are obtained if the bacterial-yeast mixture is not of a too high con- centration. Also quicker results are obtained if consecutive fields are counted in the place of a number of squares in the Hayem-Nachetsche chamber. The author recommends that in ease the bacterial suspension is too concentrated, to take 0.2 ce. of the same and 2 ce. of the yeast suspension and add 5 ee. of water. After this mixture is carefully shaken 6 drops are placed on a slide with the aid of a pipette. After drying and staining of this preparation, the counting is undertaken. The advantages of this method over Wright’s method are that we do not have to depend on the human blood, which in itself must be carefully handled and also is subject to considerable fluctuations. Besides, the standard yeast cell fluid is always ready for use. A. EICHHORN. REPORT OF THE DEPARTMENT OF VETERINARY SCIENCE. Indiana Sta. Rpt. 1920, pp. 43-47. Abs. in Exp. Sta. Ree., vol. 45 (1921), No. 2, p. 176. Hog cholera feeding experiments failed to demonstrate the presence of the virus in the urine and feces of hogs having acute cholera. In infection experiments, healthy controls, sepa- rated from pigs inoculated with cholera blood by wire partitions placed 3 feet apart, contracted the. disease in from 17 to 25 days.. Numerous attempts made to isolate Bacillus suipestifer from the feces of hogs affected with cholera and dysentery proved unsuccessful. Pigs that were fed cultures of B. suipesti- fer sickened and developed diarrhea, but in no case was B. suipestifer recovered from the feces, although an organism somewhat resembling it was constantly isolated. The adminis- tration of thymol-chenopodium in capsules failed to prove of value in removal of intestinal worms. In an outbreak of a disease in horses that resembled forage poisoning, in which 16 of 32 horses on a farm died between February 15 and March 2, an organism morphologically similar to B. botulinus was obtained from the cecum of one horse, but it did not produce toxin. Botulinus antitoxin of the B type 334 ABSTRACTS was administered subcutaneously in doses of 40 to 80 ece., re- peated in two days, but did not seem to prevent the disease or retard its progress. The preparation of chicken-pox vaccine was begun early in the year, 1,286 birds in six flocks being tested with satisfactory results. A summary is given of the laboratory diagnoses made during the year, and reference is made to the production of antihog-cholera serum and serum tests and to agglutination and bacterin tests. ON THE VALUE OF Non-SPECIFIC IMMUNIZATION IN THE ERADICA- TION OF VIRULENT Foot-ANb-MoutH Disgease. KE. F. Muller, Schweiz. Archiv. fur Tierheil., vol. 63 (1921), p. 326. The great epizootic in 1920 brought out the fact that the treatment with serum from recently recovered animals confers no greater immunity than normal serum injection. Favorable results were obtained in thousands of cases treated with intra- muscular injection of 100 cc. of ‘‘ Aolan,’’ a non-specific remedy, causing neither febrile nor anaphylactic reactions. Sympto- matic treatment must not be omitted. The enormous loss of water, which unfavorably influences the heart and intestinal tract, must be replenished and general care taken along with the non-specific treatment. (Aolan is a sterile solution of milk albumin ste ocated by E. F. Muller for treatment of sykosis parasitaria, furunculosis and other staphylococcus skin infections. ) W. N. Bere. A CASE OF CANCER OF THE STOMACH IN THE Horse. D. Kramell, Berl. Tier. Woch., 1919, p. 259. A 14-year-old gelding gradually became emaciated, extending through a period of 10 months. The animal showed symptoms of chronic gastric catarrh. On post-mortem examination a cleft, semi-spherical tumor of about 35 em. in length, 22 em. in width and 15 em. in thickness, was found in the esophageal portion of the stomach. Microscopical examination revealed the tumor to be a flat-celled epithelial cancer, with the formation of cancer pearls. J. P. O’LEary. ee ee, ee oe ; é ; re Mag Tae ty gE at oer ee She ABSTRACTS 335 THE DtAGNosis oF ANTHRAX THROUGH THE EXAMINATION OF THE BoNE-Marrow. W. Pfeiler. Deut. Tier. Woch., 1919, p. 110. The eultural examination of the bone-marrow recommended by Wulff in the year 1912 had proved reliable, when the testing of other organs failed, owing to putrefaction. On the other hand, the microscopical tests of the bone-marrow and the animal tests were not always a diagnostic certainty, particularly so in the case of the microscopic examinations. The bacteriological examinations are rendered more reliable by means of the plate procedure. At the same time, according to Pfeiler, the precipi- tation method should be used in all cases as a supplemental aid in diagnosis. J. P. O’LEary. CHAULMOOGRA OIL IN THE TREATMENT OF TUBERCULOSIS. W. S. Culpepper and M. Ableson. Jour. Lab. and Clin. Med., Vol. VI (1921), p. 415. Abs. in Amer. Rev. of Tubere., Vol. V..(1921),-p. 124. The similarity in morphology and staining characteristics of leprosy bacilli and tubercle bacilli, and the specifie action of chaulmoogra and its related oils on the acid-fast group of bae- teria, furnish a scientific basis for using them in the treatment of tuberculosis. The acid fractions of chaulmoogra oil, obtained by the method of Hollmann and Dean and modified by Aldrich, were used in the following experiments: Forty-eight guinea pigs were divided into 5 groups: (1) 12 pigs were inoculated with human type tubercle bacilli and left without further treat- ment as tuberculosis controls; (2) 12 pigs were similarly inocu- lated and divided into groups of 3 each, which were given intro- peritoneal doses of the acid sodium salts of the four fractions, A, B, C and D, of acids of chaulmoogra oil; (3) 8 pigs were left as entirely untreated controls; (4) 12 non-tuberculous pigs re- ceived the same treatment as the tuberculous pigs of group two; (5) 4 pigs received increasing amounts of the A, B, C, and D fractions in a toxicity test. This report is described as pre- liminary; but the following findings are given: One per cent solutions of the acid sodium salts of the four fractions are least irritating and are readily absorbed from the peritoneum. No pathological findings could be attributed to the drug. On the other hand, it seemed to cause an increase in weight. Ns ee mn ARMY VETERINARY SERVICE 345 hind the division, such as the surgical, evacuation, convalescent and general hospitals and other medical department units of the communication zone and zone of the interior, as well as special units concerned with the mobilization and administra- tion of the medical department service. All Reserve Officers, however, residing in a Corps Area are under the administrative control of the Corps Area Com- manders. AcTIvE Duty According to law no Reserve Officer shall be employed on active duty for more than 15 days in any calendar year with- out his own consent, except in time of a national emergency ex- pressly declared by Congress. So far as practicable the per- sonal desires of officers to be called to active duty will be con- sidered when funds for training for only a portion of the Reserve Force in any year are available. The funds appro- priated by Congress are at the present time insufficient to train any considerable number of Reserve Officers, even volunteers. Only volunteers will be detailed to active duty, funds per- mitting, for instruction at Service Schools or with the Regular Army for a period of more than 15 days. Exemption from active duty. A Reserve Officer called to active duty for training purposes during any calendar year and upon whom such duty, for business or other good reasons, would work a hardship, will, upon request, be exempt from such tour of duty. Pay AND ALLOWANCES (ON AcTIVE Duty) Same as for officers of the Regular Army in the same grade and with the same length of service, and mileage from his home to his first station and from his last station to his home. Re- serve Officers are not entitled to retirement or retired pay. TRAINING, The training program will be announced annually when ap- propriations have been made by Congress. It is the aim of the War Department to establish training facilities for 15 days every calendar year for Reserve Officers who can, with- out hardship, devote this time to training. Additional train- ing, consisting of long periods of instruction at Service Schools and duty with the Regular Army and participation in Regu- ) 346 ARMY VETERINARY SERVICE lar Army activities, may be added to this program from time to time. PROMOTION A uniform system of promotion alike for all branches of the Reserve has been established. For all sections of the Officers’ Reserve Corps promotions may be made under the following conditions: (a) An officer must signify his willingness and must be ree- ommended to be examined for promotion. (b) An officer must have served the prescribed minimum time in the grade from which promotion is contemplated. (c) An officer must have satisfactorily passed the promotion examination. Minimum time to be served in each grade. To be eligible for promotion to any grade an officer must be credited with three years’ service in the next lower grade, one year of which must in every case have been in the Officers’ Reserve Corps since November 11, 1918. In computing the required three years’ service in any grade, there will be credited service in that grade in the Officers’ Reserve Corps since November 11, 1918, and active service in the same or higher grade in any compo- nent of the United States Army between April 6, 1917, and December 31, 1920, double time being given for such of the above active service as was rendered during the period of hos- tilities; 27. e., between April 6, 1917, and November 11, 1918. Except for the one year which must have been in the Officers’ Reserve Corps, there will also be credited service as a federally recognized officer of the National Guard since November 11, 1918. No service of any kind prior to April 6, 1917, is to be credited, nor is any but active service between April 6, 1917, and November 11, 1918, to be credited. Examination. Conducted by Corps Area Gonnianders by board of three officers not lower in grade than that of the ap- plicant. Scope of the examination. (a) Physical fitness; (b) moral character; (c) general fitness; (d) professional fitness. The professional examination consists in: 1. Basic military subjects, essential to all branches of the service for all officers of the Medical Department: ee eer ee ET ARMY VETERINARY SERVICE 347 Subject Scope Administration. General knowledge of regula- tions and care and use of Gov- ernment property as applica- ble to the officer as an individ- ual; channels of correspond- ence. Customs of the service, cour- The essential customs of the tesy and military discipline. service and the courtesy ex- pected of all officers; the pur- pose of discipline and the best means by which maintained. Military hygiene. Personal hygiene in the field and the conservation of health. Practical efficiency. Ability to put to practical use, in the capacity for which being examined for promotion, the knowledge possessed. 2. Special subjects essential to grade and office to which pro- motion is contemplated. For veterinary officers this will con- sist in: Recent progress in veterinary medicine, surgery and hygiene— animal and meat and dairy. SUMMARY The Veterinary Reserve Corps is thus to be organized and ad- ministered in the interests of veterinary military preparedness and by regulations drawn up by a committee prescribed by law upon which the Reserve Corps was well represented. Being a national asset to be used in an emergeney which in modern times seems to call for the mobilization of practically every ac- tivity in the country, it should have the support of the entire veterinary profession. Jf the veterinary profession can not sup- port it as thus organized, it should demand that it be so organized as to warrant its support. In compliance with law the Secretary of War has provided 1. For the organization of units of the organized reserve of a strength considered sufficient for first-line defense. 2. For the enrollment of Reserve Officers. 3. For the classification of officers with appropriate assign- ments according to special qualifications. ARMY VETERINARY SERVICE Se) ving (ora) 4. For a uniform system of promotion. 5. For a training program, largely voluntary, which will ¢ so far as Congressional appropriations permit, and at the same time safeguard the civilian practitioner of veterinary medicine from compulsory active service in time of peace when such serv- ice would work a hardship. The variety of veterinary units to be organized would seem to offer an opportunity to every veterinarian to list his services in the general scheme of veterinary preparedness. Brigadier-General Frey, formerly Director General of the French Army Veterinary Corps, who was recently placed on the retired list by the Minister of War, will make his future home in Paris. The graduating classes of 1921 at the three French national veterinary schools numbered 136, of whom 63 were at Alfort, 37 at Lyons, and 36 at Toulouse. The total number of students at Alfort at the close of the last school year was 235, and at Toulouse 151. Thirty-seven at Alfort and 13 at Toulouse were from other countries. We have seen no statement of the at- tendance at Lyons. A large number of French veterinarians have recently re- ceived honors from the French Government in recognition of meritorious service of various kinds—educational, research and military. Prof. Dechambre of Alfort, and Prof. Girard of Toulouse, have been awarded the cross of the Legion of Honor, and the educational distinction of ‘‘Palmes Acadé- miques’’ has been conferred on Prof. Arloing of Lyons, and Prof. Panisset of Alfort. JUST FOR FUN A schoolboy wrote an essay on cats. The chapter on different breeds supplies the following information : ‘*Cats that’s made for little boys and girls to maul and tease is called Maltese cats. Some cats is known by their queer purrs —these are called Pursian cats. Cats with bad tempers is called Angorrie cats. Cats with deep feelin’s is called Feline eats.’’ —Selected. ASSOCIATION NEWS AMERICAN VETERINARY MEDICAL ASSOCIATION Proceedings of Fifty-Eighth Annual Meeting, Denver, Colo., September 5 to 9, 1921 (Continued from the November JOURNAL) TUESDAY AFTERNOON, SEPTEMBER 6, 1921 GENERAL SESSION The meeting convened at 1:30 p. m., President White pre- siding. PRESIDENT WHITE: The first item of business for the af- ternoon is an additional report of the Executive Board, which will be presented by Secretary Mayo. Report OF EXECUTIVE BOARD SECRETARY Mayo: The following applications have been re- celved since the previous meeting of the board. They have been acted upon by the board and are favorably recommended : Dr. A. E. Anderson, Grand Island, Nebr. Dr. Hudson Chadwick, Jackson, Miss. Dr. T. B. Carter, Portland, Oreg. Dr. B. C. Taylor, Hillsboro, N. Dak. Dr. D. L. Zee, Minotte, N. Dak. Dr. A. J. Matelle, Crystal, N. Dak. Dr. W. H. Hilts, Elko, Nev. Dr. F. M. Coombs, Hamiota, Manitoba. Dr. W. R. Winner, Alma Center, Wis. Dr. Kenneth J. Moye, Hayes, Kans. Dr. LaFayette J. Read, Indianapolis, Ind. Dr. J. G. Townsend, Racine, Wis. Dr. J. A. Sluss, Rhodell, W. Va. Dr. George W. Neff, Charleston, W. Va. Dr. S. C. Ross, Plainville, Tex. Dr. W. E.- Norden, Avoka, Iowa. Dr. John D. Thrower, Denver, Colo. Dr. W. A. Stephenson, Salt Lake City, Utah. Dr. E. J. McBride, St. Paul, Minn. Dr. George J. Cline, Omaha, Nebr. Dr. W. L. West, Belfast, Me. ; Dr. J. B. Taylor, Brookings, S. Dak. Dr. H. H. Brown, St. Louis, Mo. Dr. G. A. Bostrum, Wahoo, Nebr. Dr. I. B. Boughton, Urbana, III. Dr. V. V. Shattuck, Omaha, Nebr. Dr. L. C. Cummings, Omaha, Nebr. Dr. C. C. Officer, Grapeland, Tex. Dr. J. Pugh, Plainville, Kans. Dr. Floyd Cross, Fort Collins, Colo. Dr. W. H. Johnson, Denver, Colo. Dr. F. A. Laird, Springfield, Il. Dr. William Harvard, St. Augustine, Fla. 349 350 ._ Procrepines A. V. M. A. Dr. Hoskins: I move that the recommendation of the Execu- tive Board be favorably acted upon, and the men whose names have been read be admitted to membership under suspension ot the rules, the Secretary to cast the ballot. (The motion was seconded by Dr. Glover and earried, the ballot was cast, and the applicants were declared elected to membership. ) SECRETARY Mayo: There is the resignation of Dr. R. M. Bell of Buffalo, N. Y. The Executive Board recommends that it be accepted. (It was voted, on motion of Dr. Glover, seconded by Dr. Adams, that the report of the Executive Board be accepted.) ELECTION OF OFFICERS PRESIDENT WHITE: The next item of business is the election of officers. The Chair is ready to receive nominations for the following officers: President, five vice-presidents and a treasurer. Nominations will be made from the floor. Nomina- tions for president are now in order. Dr. Bei: I nominate Dr. Ferguson of Wisconsin. Dr. JENSEN: In rising to second the motion of Dr. Bell, I desire to call the attention of this Association to the urgency of at this time electing a practitioner to this office. This of- fice has been filled by men outside of the rank and file of practitioners. It is not my purpose at all to give you to un- derstand that we don’t allow the State school men and men engaged in other lines of endeavor, but in order to support and increase this institution and increase its membership I be- lieve at this time a practitioner should be elected. There has been during the last year, I understand, a decline in the mem- bership, a dropping out of over 300. We have a total mem- bership of something like 3,900 where we should have 7,000. The only way I know of to stimulate interest and increase membership is by electing a practitioner. Therefore, I again take pleasure in seconding the nomination of Dr. Ferguson. Dr. GLovER: I wish to nominate Dr. A. T. Kinsley. In a small western town there was a merchant who ordered a bill of goods from a wholesale firm in a distant city. The goods were received, but were not satisfactory. They were returned. The first of the month a bill came. It was returned with the merchant’s reason. The wholesale firm then sent the bill to the city attorney. Then they sent a sight draft on the bank. Finally the wholesale firm received a letter to this effect: ‘‘I am the merchant that got your bill of goods that were not satisfactory. I am also the city attorney in this town. I am also the banker in this town, and if it weren’t for the fact that I am also the preacher in this town, I would tell you to go to hell.’? (Laughter.) ARAN el a are Nay Pele i a =a) \ SOLER SLOPE Proceepines A. V. M. A. 351 Dr. Kinsley represents every side of the veterinary profes- sion. As an educator he helped to build up the largest and one of the best veterinary colleges in the world. As an author, his texts are used in the veterinary colleges, and his books of ref- erence are found in the library of almost every veterinary surgeon in this country; as a manufacturer of biologics, he has taken a prominent place. As a counsellor in veterinary lines, he is called to every part of the United States. He repre- sents the profession in a large way. We all know Dr. Kinsley. The only objection I have heard anywhere to Dr. Kinsley is the fact that he is interested in commercial enterprises. Can you think of any reason on earth why that should be an objec- tion to a man for the presidency of this Association? Dr. Kins- ley has stood four-square before the world, honorable, straight- forward, a leader in our profession. We are proud of him, and I want you to understand, as far as I know, in this district we are for Dr. Kinsley for the next President of the Association. (The nomination was seconded.) Dr. Exvrason: In seconding the nomination of Dr. Fergu- son, and speaking from the standpoint of a man from his home State, I wish to announce that Dr. Ferguson didn’t cause himself to receive this nomination, but has received several letters urging him to run for this position. After getting sev- eral such communications, we came here with a view of find- ing out the sentiment. Dr. Ferguson is an out-and-out prac- titioner. He has been’successful. He has done much to elevate the profession in his own State, and we believe him fully eapable for the office for which he is nominated. It is not our purpose to come here to be an obstacle to anyone else, or deprecate the honors which may be given someone else. The good of the Association is the thing that we should have at heart. (It was voted, on motion of Dr. Stange, duly seconded, that the nominations for president be closed.) PRESIDENT WHITE: I will appoint the following members to act as tellers: Dr. S. E. Bennett, Dr. C. P. Fitch, Dr. R. D. Marsteller. These gentlemen will kindly distribute the ballots upon which you will write your preference. Secretary Mayo: I have received the following message from Dr. Tom Smith of Jersey City: ‘‘Regret that my health will not permit attendance at the convention. The convention should not adjourn without paying some tribute to Dr. W. Horace Hoskins.’’ PRESIDENT WHITE: Gentlemen, so that you will not be kept waiting so long, and to facilitate matters, I will appoint another set of tellers to distribute ballots for the five Vice-Presidents. Unless you have some objection to this suggestion, I will ask Dr. C. H. Stange, Dr. H. P. Hoskins, and Dr. C. H. Hayes to dis- tribute ballots and act as tellers. 352 Procreepines A. V. M. A. Secretary Mayo: I want to call your attention to one sec- tion of the Constitution and By-Laws that says that no person shall be eligible to the offices of President, Vice-President, Secre- tary or Treasurer unless he has been a member in good standing tor five years preceding the election. (The ballot for President was taken and the tellers retired to make the count. ) PRESIDENT WuiItE: Nominations for Vice-Presidents are in order. Dr. ErcHHoRN: Mr. President, I would like to place in nomi- nation the name of a man who has worked the Pacific Coast con- stantly and strenuously for the veterinary profession, Dr. Mc- Kenna of Sacramento, California. (Seconded. ) Dr. Newsom: Since we started in in the far West, the next in order will be a man from Colorado. I would like to nominate Charles G. Lamb, who has been the leading spirit in the prepara- tions for the convention that is now assembled. He is State Veterinarian of Colorado. (Seconded. ) Dr. Cary: I nominate Dr. Hamlet Moore of New Orleans. (Seconded. ) Dr. Huron: I beg to nominate W. A. Hilliard of Winnipeg. (Seconded. ) Dr. Corton: I notice that the extreme Kast is not represented. 1 therefore place in nomination Dr. Robinson, Portland, Maine. (Seconded. ) Dr. Gow: I notice you have candidates from various parts of the country. I would like to nominate Dr. John Eagle of Kansas City, Mo. (It was voted, on motion, duly seconded, that the nominations be closed. ) 5 Secrerary Mayo: It has been customary for several years to make a motion that the one receiving the highest number of votes should be First Vice-President, the second highest, Second, and soon. There are five to be elected. Dr. Bett: I make a motion that that custom prevail. (The motion was seconded and carried. ) PRESIDENT WHITE: It is customary to vote for five. In case there are more on the ballot, the lower ones will be dropped auto- matically. (The ballot for Vice-Presidents was taken. ) PRESIDENT WHITE: The result of the ballot cast for Presi- dent gives Dr. Ferguson 62 votes and Dr. Kinsley 100 votes. (Applause.) The Chair therefore declares Dr. Kinsley elected President for the ensuing year. Will the same tellers kindly pass around ballots for the elec- tion of Treasurer and collect them? Nominations are now in order for the office of Treasurer. Dr. Cary: I place in nomination for Treasurer a man from a erage ress” 6.0 TH Rp ma PE A SPA ERE I SOT RRS rae Proceepines A. V. M. A. 353 the South. I don’t believe we could get a better man for the office, for they don’t get them. Therefore, I nominate Dr. Jacob of Knoxville, Tennessee. (Seconded. ) Dr. Koen: I move that nominations be closed and that the Secretary be instructed to cast the ballot of the Association for Treasurer. (The motion was seconded and unanimously carried, and the Secretary cast the ballot of the Association for Dr. Jacob.) PRESENT WHITE: I declare Dr. Jacob elected Treasurer of this Association. (Applause.) The result of the ballot cast for the five Vice-Presidents is as follows: Dr. C. G. Lamb, 157; Dr. McKenna, 135; Dr. Robin- son, 133; Dr. Hilliard, 122: ; Dr. Moore, 111. I therefore declare Dr. Lamb First Vice- President, Dr. McKenna Second, Dr. Rob- inson Third, Dr. Hilliard Fourth, and Dr. Moore Fifth. I have added to the Committee on Emblem the name of Dr. McKenna of California. REPORT OF COMMITTEE ON LEGISLATION PRESIDENT WHITE: We will have the report of the Legisla- tion Committee. (Secretary Mayo read the report of the Committee on Legis- lation, as follows:) Your committee has kept in touch with any legislation which has had any bearing on the advancement of the profession. The most important contemplated legislation has been that relative to the reclassification of the civil service of the United States. Bills have been inroduced in both Senate and House by different members of each body. These bills have been thoroughly gone over by the various committees, but to date no particular bill has been agreed upon. In one of these bills the veterinary profession was discrimi- nated against, and only by the most active work of the profession, both in and out of the Federal service, was this discrimination re- moved and the profession classified similarly to the other learned pro- fessions. The Sterling Joint Committee of Congress on Civil Service Reclassification gave a hearing to Dr. John R. Mohler, Chief of the Bureau of Animal Industry, United States Department of Agriculture, who stated that the veterinarians in the civil service were entirely satisfied with the new classification now given them in the bills as re- cently amended by the committee. Your committee has not been regularly called together to make any organized effort toward this reclassifieation legislation, as it consid- ered it untimely to waste any activity or expend any funds for propa- ganda work until the Congressional committee had definitely decided upon the character of the bill it would report out of committee. It is believed that the veterinarians are well organized to make this effort when the proper time arrives. When the call is made to the profession to back up this legislation, which affects thousands of our brethren in the various civil branches of the Federal service, it is hoped that every member will personally interest himself in this work and will so utilize his efforts that Sen- ators and Representatives in Congress will give these illy-paid public servants an adequate, living salary and not keep them in suspense from year to year by merely allowing them the war-time “bonus,” a 354 Proceepinas A. V. M. A. sort of act of charity by Congress. The efforts of the profession should be directed toward personal interviews with Senators and Representatives, inviting their attention to the very inadequate salaries now paid veterinarians in the civil service and the great loss sustained by the Government every year by the resignation of many highly trained veterinary specialists, owing to the low salaries paid them, and their replacement by untrained men who can not be of much value to the service for several years. Much good can be directed toward these efforts by veterinarians seeking the aid of livestock associations, breeders’ associations, women’s clubs and various civic centers. Your committee is thoroughly aware of the fact that without the aid of each veterinarian it can not be successful in its efforts. Per- sonal activities of veterinarians in their home districts have far more effect on molding the opinions of Senators and Representatives than have high-priced committees convening in Washington. Every veteri- narian has the acquaintance of several prominent men in their respec- tive sections; it is truly wonderful the effect that interviews and let- ters from such citizens have on our national legislators. Short articles in the local press do very much good when properly forwarded to Washington. The Legislative Committee is ready and anxious to further this leg- islation in all possible ways when the opportune moment arrives. JOHN P. TURNER, Chairman. S. J. WALKLEY. PRESIDENT WHITE: You have heard the reading of this very excellent report. What is your pleasure in regard to it? Dr. KreERNAN: I move that it be adopted and that the com- mittee be urged to keep in touch with the legislation now pend- ing in Congress and serve the Association and profession when- ever called upon to do so. (The motion was seconded and carried.) REPORT OF REPRESENTATIVE ON ADVISORY BOARD OF THE Horse ASSOCIATION OF AMERICA PresIDENtT WHITE: Are any other committees ready to re- port ? SecreTaARY Mayo: I have a report from Dr. Dick, who is a member of this Association representing this Association in the work done by the Horse Association of America. (Secretary Mayo read Dr. Dick’s report, as follows:) As representative of the American Veterinary Medical Association on the Advisory Board of the Horse Association of America, I want to present the following report of the work done by the Association dur- ing the past year. The first piece of work was the gathering of proof of the superiority of horses and mules in various classes of work. This was the foun- dation. It was necessary to have proof that horses and mules were more efficient and economical than mechanical motive power or their whole campaign would be useless. Trained investigators were sent to New York, Philadelphia, Boston, Buffalo, Chicago, St. Louis and numerous other cities to collect data from business men, cartage and transfer men, ice companies, coal companies and all users of transpor- tation. It was found that some men had sold their horses and pur- chased trucks, but after giving them a thorough trial had discarded Proceepines A. V. M. A. 355 them and gone back to horses. The following is what A. Moll Com- pany, wholesale and retail grocers, in St. Louis, had to say on that subject, which is just a sample of many such letters: “We found after keeping accurate records that the cost of main- taining trucks was astonishing in comparison to horse-drawn equip- ment, and the service not so reliable. At present we are using 65 head of horses and mules, and I believe as soon as other concerns now using motor transportation figure their costs they will return to horses.” The Horse Association also found that the cost per day of operat- ing a team truck with a pair of heavy draft horses that would pull five tons or more on city streets was from $4.06 as a minimum to $7.75 as a maximum, exclusive of driver. With the driver at $5.00 a day the total would be from $9.06 to $12.75 a day. Against this it was found that it costs from $25 to $30 a day to operate a five-ton motor truck, or more than twice as much as a five- ton team truck that would haul the same load. | A company of Evanston, Ill., found that for the entire year of 1919 their cost of delivery with horse-drawn equipment was 6% cents and with motorized delivery 8 1-5 cents per package. In the use of tractors on farms it was found that horse-drawn equipment was more efficient, reliable, economical and flexible than mechanical motive power. One of the most notable examples of this is the farm comprising 30,000 acres owned by the Noble Foundation Company, Ltd., of Alberta, Canada. On this farm it was found that for double disking and harrowing it cost 42 cents an acre with horse- drawn equipment, 60 cents an acre with steam tractors, and 70 cents an acre for gas tractors. This company hitches 12 horses to three grain drills and seeds on an average 75 acres per day per man. They are discarding their tractors and using horses exclusively. One of the most important facts disclosed by the investigations made by the Horse Association of America is that fixed charges on trucks standing idle exceed the cost of feeding horses with equivalent work- ing capacity. The motor salesman has usually made the claim that it cost comparatively little to keep a truck when it was idle. This should be a serious blow to motor interests. The next step was to determine the users of horses and mules in non-agricultural work. A list comprising the names of 204,000 firms has been compiled, as follows: Coal dealers with over $10,000 capitalization.................. 8,746 Teaming and transfer COMPANIES... 7,150 Retail grocers rated over $1,000. esecessessssseeeanee 59,142 Ta PEAS eel 2A a of 06 2H ele aa eho MRA a a 3,827 Ue) Sy Ei ah 2700 ARS) a eS ee a a 4,688 TEayaliveres) og Aes cas eapae een enl ofe ane ne el. 2 eee eee 388 TICES 6 (EEN Sok SR a io < oh ia aren 6,011 PR Ree ATC GR DTOSS COMM ADIOS eat chet 11,140 ITE CRIES SO Re ee eS ee eee 10,167 Pere CON LTACHOTS +..204... co st ae ee eS 762 Graveleandssandscompanieses...00512 oko eds oes 1,350 Bakeries with over $1,000 capitalization... 27,476 Heavy hardware (wholesale) .......ec-cscccscssscsccsseeseseseeseereeeeam 1,217 Wamp SRM EATCESier. Cer so 0) tbr es ews Bee ee ace 27,022 Lumber manufacturers (sawmills) 0.00 16,579 Wines. Otwalllevicnnd Site 8 25 S| a ead. 18,400 = AR Gye LA Fee) oa Re ae BD St a Rh A OA 204,065 356 Proceepines A. V. M. A. The first seven groups had been reached on December 1, 1920, with personal letters and circulars dealing with their particular prob- lems. A supplementary distribution of literature has been made as fol- lows: Saddlery firms and their salesmen. Hay, grain and feed dealers. Horseshoers. Horse and mule dealers. Wagon and buggy makers and dealers. Veterinarians (list of 4,000). Country bankers, 23,364. Another important piece of work undertaken by the Horse Associa- tion of America is that of encouraging the production of horses. It has been found that there has been a tremendous decrease in the pro- duction of horses in the great horse-producing States. When we real- ize that the eastern and southern parts of the United States depend on this section for their horses we can appreciate the seriousness of the situation. These horse-producing States are not only falling down on the production of new horses, but those now found on a large per- centage of the farms are four years old or over. This means that unless farmers begin to produce horses at once a serious shortage is bound to come suddenly and the prices will be exorbitantly high. This will be to the advantage of the motor interests. The Horse Association is reaching the farmers by circularizing with letters and leaflets the leaders of farm sentiment. This list is as follows: County agents and farm bureau officers. Stallion and jack owners and leading horse breeders. Bankers in all towns under 30,000. Auctioneers and horse buyers. Country harness dealers, horseshoers, wagon and carriage dealers. Hay and grain dealers. State fairs and State boards of agriculture. State stallion enrollment boards. In addition to this, contributions have been made to the agricultural press and to the weekly news service of 1,250 newspapers. This rep- resented up to December 1, 1920, 9,490.5 linear inches reaching over 12,000,000 people. Another line of work undertaken is the stimulation of the use of horses and ponies in the field of healthful recreation and sport. This included investigations of existing facilities for horseback riding in many of our cities and securing information from noted physicians on the influence of horseback riding on the maintenance of health and vitality in man. The co-operation of the American Remount Associa- tion, the Chicago Equestrian Association, the American Saddle Horse Breeders’ Association and other saddle horse interests were secured in a nationwide campaign to provide more extensive facilities for horse- back riding throughout the country. An effort is also being made to stimulate interest in driving horses for business and pleasure. Another phase of the work done by the Horse Association of Amer- ica is the stimulation of production of the right kind of horses. This is being done by literature prepared to show that profitable operation and marketability are dependent upon qualities of efficiency; by cash prizes offered at county fairs, the fairs duplicating the Association’s money, and, finally, by direct work of field men who visit leading breeders and farmers, with whom they discuss this problem. Procerepines A. V. M. A. SE Up to December 1, 1920, a total of 1,100,000 pieces of literature con- taining the above-mentioned facts had been distributed to men who were interested in efficient, economical motive power units. These letters indicate to some extent the tremendous amount of work done by the Horse Association during the past year. Mr. Dinsmore, in a letter addressed to me on July 25, 1921, said: ‘Since the last annual meeting we have distributed a little more than one million and a half pieces of literature, and all of this material has gone straight to the men who are directly interested.” This work is apparently producing good results. It is reported from Boston that the sales of horses made by one man during the first four months of this year were over 5,000 head, as against approximately 4,000 head in 1920 for the same months, a gain of 25 per cent. A firm in New York City sold during the first five months of this year 14,239 horses as against 11,605 in the same period of last year. In Chicago the United States census, taken January 1, 1920, reported 30,388 horses. The licenses issued this year to June 1 show that there were approximately 33,660 horses in use in Chicago last spring, a gain of 3,000, or a little more than 10 per cent. Such excellent work should receive the hearty support of every veterinarian. In Pennsylvania the State Veterinary Medical Association and some of the veterinary clubs are actively supporting this Association. Every veterinarian should become an active member. To this end an active membership campaign was launched in Pennsylvania with gratifying results. The good work of the Horse Association during the past year will no doubt be fully appreciated by the American Veterinary Medical Association, and it is hoped it will again actively lend its support to this work and appoint a member to represent it on the advisory board of that organization. G. A. Dick PRESENT WHITE: What shall be done with this very excei- lent report? (It was voted, on motion of Dr. Koen, duly seconded, that the report be accepted. ) REPoRT OF COMMITTEE ON NECROLOGY PRESIDENT WuHitr: I will now eall for the report of the Com- mittee on Necrology, Dr. Connaway, chairman. Dr. Connaway: I regret that we can not make a full report at this time. Some of the members of the committee to whom part of this work was assigned have not reported, but we will eet the full report and file the data with the Secretary. It seems to me appropriate at this time to have a little me- morial service in honor of some of our distinguished members who have died during the past year, Dr. W. Horace Hoskins, Dr. James Law, Dr. E. A. A. Grange and Dr. F. S. Musselman. It has been suggested that Dr. Moore say a few words in regard to Dr. Law; that Dr. Adams speak concerning Dr. Hoskins; that Dr. Mayo and Dr. McGilvray say a few words in regard to Dr. Grange; and that Dr. Dimock respond for Dr. Musselman. PRESIDENT WHITE: You have heard the suggestion of the Chairman of the Committee on Necrology. Is Dr. V. A. Moore here? 258 Procerepines A. V. M. A. TrispuTE To Dr. JAMES LAW Dr. V. A. Moors: Those of us who have known Dr. Law, who have been his students and who have worked with him, have come to know the breadth of his knowledge, the interest he had in his profession and the sympathies he entertained for those who are striving to fit themselves for veterinary work. Dr. Law was born in Edinburgh, Scotland, in 1838. He was graduated from the Veterinary College of Edinburgh. Fol- lowing his graduation he studied human anatomy under William Turner and surgery under Joseph Lister, the father of antiseptic surgery. Later he studied at the great veterinary colleges of Lyons and Alfort. About 1860 he became professor of veteri- nary anatomy, physiology and materia medica in the New Veter- mary College of Edinburgh and its continuation in London as the Albert Veterinary College. Besides his teaching, he prac- ticed his profession in Scotland, in England, and for one year in Belfast, Ireland. In 1868, at the solicitation of the Hon. Andrew D. White, he came to Cornell University to accept the position of professor of veterinary medicine and surgery. He was a member of the original faculty of the University and one of its most esteemed and influential men. He occupied the position as head of the department of veterinary science in the University until 1896, when he became director of the New York State Veterinary Col- lege established at the University, which position he held until 1908, when he retired at the age of seventy. He was appealed to by both the State and Federal Govern- ments in connection with the study and control of serious dis- eases of domesticated animals. He was a pioneer in America in putting veterinary medicine on a sound scientific basis. He gave valuable assistance to the Federal Government by his investiga- tions in connection with hog cholera. Later he was sent by the United States Department of Agriculture as its representative to the International Veterinary Congress at Brussels, Belgium. He took an active part in the eradication of contagious pleuro- pneumonia from the United States. Dr. Law was a thorough student. He was also an extensive writer on veterinary topics. For many years he was an author- ity in practically all branches of the science. He was skilled in diagnosis, a thorough anatomist and a successful surgeon. In addition to the valuable work he did as an investigator and practitioner, he was an inspiring teacher. There is a no more effective inspiration than that which has come from the work and life of Dr. Law, who, through his genius, his devotion to truth and his kindly acts, has demonstrated the power of man to succeed as a gentleman, a scholar and a leader. He exemplified the simplicity of suecess in righteous living and faithful, honest endeavor. Proceepines A. V. M. A. 359 Dr. Law recognized that the animal husbandry of this country would soon require the services of a large number of scientifically trained veterinarians to combat the numerous destructive dis- eases of livestock that were beginning to gain a foothold here. He stood for higher veterinary education and adequate livestock sanitation. By his influence and personal efforts, the first State- supported veterinary college was established. Through his students of earlier years, over whom he had a profound influ- ence, the Federal Bureau of Animal Industry was organized in 1884, and livestock sanitary boards were created. Dr. Law was an idealist whose purpose and ambition in life has been the advancement of a cause rather than self. Like the great Pasteur, the work he was advancing stood between himself and the public. His pupils remember him as a master and also a man possessed of much human sympathy. With all his great- ness he was a manly man with a sweetness of disposition rarely found among men. He will go down in history not only as a scholar, teacher and leader, but also as a man of kindness, cour- age and truth. TRIBUTE TO Dr. W. Horace Hoskins PRESIDENT WuHiTE: Dr. Adams will speak in respect to the late W. Horace Hoskins. Dr. Adams: Last night, Dr. Connaway, Chairman of the Committee on Necrology, asked if I would say a few words in memory of Dr. Hoskins. I suggested that there were other men who had been more closely associated with him than I, and who possibly could more appropriately speak for him, but that weak argument was overruled, and I will not try to evade what I con- sider is a duty. I am not familiar with all the salient facts in his professional life, but I knew the man as a man, and as such I would try to present him to you. He graduated from the American Veter- inary School early in the eighties and pursued practice continu- ously in Philadelphia until some two years ago, when he ac- cepted the presidency of the Veterinary College of the University of the City of New York. His one passion in life, it seemed to me, was to organize the veterinary profession into working societies. He did not con- tribute anything notable in the way of veterinary discoveries. He was not an outstanding practitioner, but he was outstanding in exemplyfying the passion I have spoken of—the passion for getting old and young veterinarians together and getting them acquainted, organizing and inaugurating laws for better control of the profession and better relations between the profession and the public. That has been his life work. He has attended con- tinuously, I believe, thirty-eight meetings of the A. V. M. A., has not missed one year in thirty-eight or possibly thirty-nine. He 360 Proceepines A. V. M. A. has held nearly every position of honor in this Association. He was for many years Secretary of the A. V. M. A. He was always a prominent member upon its Legislative Committee. He gave of his time unstintedly, | believe, more than any man who has ever belonged to this American Veterinary Medical Association. Night and day, early and late, he had it in his mind. As I said before, it was a passion with him. Now that he is gone, I hope somebody will be raised up to carry on his work in the spirit in which he carried it on. He meant a great deal to this Associa- tion in its early days, and from now on we shall miss him. ( Applause. ) TRIBUTE TO Dr. E. A. A. GRANGE PRESIDENT WuitE: Dr. Mayo will pay tribute to the late Dr. Grange of Toronto. : ; SEcRETARY Mayo: When I became a student of Michigan Agricultural College in 1884, Dr. Grange was giving lectures on veterinary science in that school. I think he had recently come from Minnesota, where he had lectured for a time at the Uni- versity of Minnesota. As a farm boy I was quite interested in horses, and Dr. Grange was a typical Englishman in his love for animals and particularly for horses. It was a mutual interest that brought us together in a way, and it was largely through his influence that I took up the study of veterinary medicine. I was under him a year as a student, and after I had completed my veterinary course I was appointed his assistant and was in- timately associated with him for two years. He was a most de- lightful man to be associated with. I never had to ask him for anything; he always asked you if you wouldn’t like so and so. He was a perfect gentleman, a pleasing lecturer, and I remember was several times voted by the students the most popular pro- fessor in college. He was instrumental in getting through the Legislature of the State of Michigan the law providing for a State Veterinarian, and he was the first man appointed to that position under the law, and held it, I believe, until he resigned in 1897 to go into other work. I have always had the highest regard for Dr. Grange because of his sympathy and his perfect gentlemanliness. He was born in England, and came from a very fine family, but he was edu- lated largely in this country; was a graduate of Ontario. He was a very witty man, in a dry way, and those of you who had the opportunity of hearing him in California in 1910 extend an invitation to this Association to hold its next meeting in To- ronto will recall the incident when he said he wasn’t an orator, although he apparently attempted a little on this occasion. He said he wanted them to understand that Canada had mountains that reached to the skies, she had rivers hundreds of miles wide and millions of miles long, and, he said, ‘‘there is water in ’em.”’ It wasn’t so much what he said as the way he said it. ee Proceepines A. V. M. A. 361 It was my privilege a few years ago to call on him at his home in Toronto. He was paralyzed, helpless, unable to speak; and yet the same merry twinkle was in his eye as always. (Ap- plause. ) PrREesWENT WuitTE: Dr. McGilvray of the Ontario Veterinary College will pay additional tribute to the memory of Dr. Grange. Dr. McGinvray: Needless to state at this time, it was con- siderable feeling that prompted me to accede to Dr. Connaway’s request that I should also bear testimony in regard to Dr. Grange. I fully appreciate the sentiments that have been ex- pressed by Dr. Mayo here regarding the late Dr. Grange. ~ I was associated with Dr. Grange very closely since the year 1908, and during that time I learned to like him and to love him very much. The fact is that at the first glance one was apt to misjudge the late Dr. Grange. He probably hadn’t acquired that gift which some have of immediately ingratiating himself into the presence of a stranger. He was somewhat reticent; the fact is, he was reticent to a degree; but as you became more closely acquainted with him you got to know him better and then found out that underneath the surface there was a great deal of warmth and humanness that, after all, meant a great deal. In the later years of his life Dr. Grange did much for the veterinary profession and veterinary education as a whole. He undertook to take care of the Ontario Veterinary College at a time when it was being transferred from private ownership to state or government control. One can imagine the difficulties one would encounter under such circumstances. Previously this institution had been run privately, and the owner of the place, like the centurion of old, could say to his people, ‘‘Come,’’ and they came, and ‘‘Go,’’ and they went. Now, to be supervisor of an institution of that kind under government auspices you have to be guided by legislation. Dr. Grange succeeded in having proper legislation enacted governing the college, and through fhe work he did in that regard he has been able to show a clear vision for the future. Dr. Grange was held in very high esteem by the student body. They got to know him and to like him, and I must say that in all those years I have never yet been able to find the student who could say otherwise than that Dr. Grange was a gentleman. In the year 1918 he had reached the allotted span of sixty years of age, under which the Government requires all Govern- men service to be terminated. Dr. Grange in that year was re- tired and superannuated. We still continued to have him, how- ever, closely identified with the college. He felt so bound up in the work, even while he wasn’t required to be at the college at all, that he came to that institution every morning at 9 o’clock sharp. I never knew Dr. Grange to be late; I never knew him to 362 Proceepines A. V. M. A. fail to keep an appointment and be on time. It is well for us young chaps to keep in mind that punctuality is a great thing. i‘hrough the whole year he continued to take active interest and devote his entire time to the work of the college. Unfortunately, he was attacked with what we ordinarily call a stroke. During the previous year he suffered from an injury he received through falling from a horse. When he became bedridden, | found him, as Dr. Mayo said, game to the core. I will say for Dr. Grange, if ever a man was game even in sick- ness, it was Dr. Grange. I saw him a few weeks before his death, and he was the same cheerful old fellow, always wanting to know if there was something he could do. He was a real gen- tleman and one of Nature’s kind. (Applause.) TRIBUTE TO Dr. S. F. MusseLMAN PRESDENT WuitTE: Dr. Dimock of Kentucky will speak in memory of the late Dr. Musselman. Dr. Diwock: I know there are a number here who knew Dr. Musselman for a longer time than I did, and are therefore bet- ter prepared to speak on this occasion. Some of you will remem: ber that there was a memorial of the late Dr. Musselman at the meeting of the United States Livestock Sanitary Association last December, and I would suggest that some of the things that were said at that time regarding his life work be used by the Com- mittee on Necrology in its report to this Association. At this time I can only repeat a few of the points that were made then. As you know, I have been in Kentucky only a little over two years, and while I had met Dr. Musselman at Association meet- ings, my acquaintance with him dates from July 1, 1919. There- fore what I say is from my own brief acquaintance with him and what I hear from those who knew him for a longer time. His work in the profession was that of a practitioner and State official. Of his activities in the profession we hear nothing but praise, and it seems that as time goes on, since his death, those who knew him best realize more and more the value of the man in his work. I am well acquainted and come in contact almost daily with some of the members of the Livestock Sanitary Board of Kentucky, and very frequently during the past few months, different ones have remarked to me how they miss him in the work. He was the leader of the Livestock Sanitary Board of Kentucky, and the members of that board had come to depend upon him as an adviser in directing them in their field of ac- tivity. Dr. Musselman was an ideal man to work with and appreciated fully the value of cooperation. In our work at the university on animal diseases, friction with the State Department at Frank- fort has been unknown; quite to the contrary, we could always depend upon Dr. Musselman for support. The men who were career Procerepinas A. V. M. A. 363 under him as assistant State veterinarians looked upon him as a friend and adviser, much as young men look to their fathers for counsel. The veterinary profession of the State of Kentucky felt that in Dr. Musselman they had a man whom all could accept as the type of professional representative of the best of our profession. He was the standard bearer of the profession in Kentucky. We miss him and honor his memory. (Applause.) PRESIDENT WHITE: It would seem appropriate to close this little memorial service by having us stand as a body and in silence until you hear the fall of the gavel. These were eminent men, eminent fellow members who have passed from among us, who can never meet with us again, and on this earth at least whose faces we can never see again. I therefore ask you to stand in silence for a few moments. (The audience arose and stood in silence for a few moments. ) GREETING TO Dr. ARCHIBALD Dr. BENNETT: If it is in order at the present time, I would hke to suggest that this Association now assembled in Denver send a telegram of greeting to Dr. Archibald, who has been un- able to attend our meetings for the past two or three years on account of illness. He is a member of the Executive Board from the Fifth District. I think it would be well at this time to have the Secretary send a telegram of greeting to Dr. Archibald at his home. (The motion was seconded by Dr. Kiernan and carried.) REPORT OF THE COMMITTEE ON BOVINE TUBERCULOSIS PRESIDENT WHITE: Is the Committee on Bovine Tubercu- losis ready to report? (Dr. Cotton read the report of the committee, as follows :) Bovine tuberculosis, notwithstanding the long fight that has been directed against it, continues to hold its unenviable, high rank among the costly infectious diseases that plague our food-producing animals. But this should not discourage our efforts to combat it, as the dangers and losses for which it is directly and indirectly responsible have never before been better recognized by those who are interested, through either altruistic or selfish motives,in the conservation and per- fection of the animal sources of our food supply, and the eradication activities of our Federal and State Governments have never been more active or promising. The outlook is so encouraging that we should unitedly try to dispel the gloom that too often clouds discussions on eradication, by cheerfully teaching that tuberculosis among animals can be wholly and permanently suppressed, and that we are moving toward its suppression as rapidly as the available men and means permit. We should not assert or claim that the end can be reached in a year or two, because that would encourage false hopes and tend to discredit our judgment. The eradication of a disease which, in the United States alone, concerns more than 23 million dairy and nearly 43 million other cattle, requires time, and the relatively small number 264 Procreepines A. V. M. A. of competent men who can be called on to test and examine this vast multitude of animals, even if unlimited sums of money were available, would prove an important factor in determining the rate at which progress can be made. In addition to money and trained men, eradication also requires the proper education of livestock owners, on whom dependence must be placed more and more in the future, as the number of cleaned herds and areas increases to protect such herds and areas against reinfection. Without the aid, interest and good will of the livestock owners it is questionable whether a widespread, common, insidious, usually chronic, often long-concealed disease like tuberculosis can be conquered, as it is their authority through which the enforcement of many primely neessary measures must be secured. The following important facts about bovine tuberculosis and its eradication should be widely published: a. The bovine tuberculosis eradication work that is now being done is encouraging, and the results that are being obtained promise to be permanent. b. The officers of the Federal and State cooperative tuberculosis eradication activities now are receiving more requests from cattle owners who desire to have their herds accredited free from tubercu- een they can attend to with the men and money at their com- mand. ec. The sooner bovine tuberculosis is eradicated the less the work will cost, and the sooner the losses it causes will stop. d. The frequency of tuberculosis among the cattle of a country is closely proportioned to its number of cattle and the period of time the disease has been permitted to spread without a real fight to con- ral it, and an effective fight is impossible without the use of tuber- culin. e. Before tuberculin was discovered tuberculosis probably had reached nearly, if not quite, its present frequency among European cattle, which is from three to five times as great as among American cattle. A comparison between our older and younger States shows that the percentage of tuberculous cattle is from 25 to 30 times as great in some of the older than it is in some of the younger. f. Eradication of bovine tuberculosis in Europe, because of the much larger proportion of infected cattle, is a decidedly more difficult and expensive thing than it is. in America. _We should not permit the cattle in our older States to become as commonly infected as those of Europe, or those in our younger States in this respect to equal those in our older States. Indifference and dilatoriness will be per- niciously effective in bringing about such undesirable conditions. g. A half-hearted, poorly financed campaign against bovine tuber- culosis is not sufficient, as it does not promise, to judge from experi- ence, to remove existing cases and centers of infection as rapidly as new ones develop. h. The losses caused by bovine tuberculosis may fall more heavily on some persons than on others, but they are finally paid by the peo- ple as a whole, as the disease increases the price of all beef and dairy and pork products. If the increases were payable in a lump sum, once per annum, in the place of being paid, as is now the case, in one or more daily installments, the eyes of those who are being taxed probably would open wide with wonder. Recognizing the truth of these statements, and using them as a basis, the committee recommends that the following resolution be ap- proved by the Association: “Whereas, Bovine tuberculosis is a widespread and destructive plague of cattle and hogs, and is, moreover, responsible for. many cases of tuberculosis among children; en wow a a agente Proceepines A. V. M. A. 365 “And whereas, The excellent work now being done to eradicate this plague is being hampered by lack of funds and for some time to come will require annually increasing sums of money to insure that it may keep pace with the requests received from cattle owners to have their herds cleaned and accredited free from tuberculosis: “Be It Resolved, That the members of this Association, who may be assumed to be peculiarly qualified, through education and the nature of their work, to understand the conditions that affect the welfare of the animal industry and the prices of food products obtained from animals, believe it to be their duty to point out that it is urgently desirable that all Federal and State appropriations for the eradica- tion of bovine tuberculosis should be annually increased, and that the annual increases should be sufficient at least to meet the growing demand of cattle owners for reasonable help to stamp out the dis- ease in their herds; “Be It Further Resolved, That this resolution shall have the widest possible distribution, either or both by being mailed to members of fee puures or by being published in livestock and agricultural jour- nals. The resolution should ke distributed from the office of the President or Secretary of the Association, and if this requires some expenditure, an appropriation for the purpose is recommended and should be made. The last annual report of the committee included a number of rec- ommendations, the approval of which imposed special tasks on the committee of this year. The first deals with the intradermal use of tuberculin as a test for tuberculosis. Studies of the test were to be made and a questionnaire sent to all the members of the Association, and by such means it was hoped the needed information would be ob- tained for the preparation of a definition of what actually constitutes an intradermal tuberculin reaction. As no money was appropriated for the preparation and distribution of the questionnaire, the expe- dient of publishing it, with a request for attention, in the JOURNAL of the Association, was used. (See JOURNAL OF THE A. V. M. A., April, 1921.) Though it could not be expected that the expedient would yield as many replies as a questionnaire mailed separately to each member of the Association, it would be a serious matter if the meager number received could be looked upon as an index of the interest American veterinarians take in matters intimately and importantly related to the eradication of tuberculosis among domestic animals: and when you know that the total number of replies lacks one of be- ing half a dozen, you will admit the truth of this statement. The committee takes pleasure in expressing its thanks to the five men who troubled themselves to show their interest in its questionnaire. However, it does not deem it advisable to draw and publish con- clusions from so few reports. Among veterinarians who have had considerable experience with the different methods of using tuberculin the belief is often expressed that it is urgently necessary that the method of making the intra- dermal test should be standardized, and that, if this were done, many errors now charged against it would be eliminated. The needles and syringes used, the amount and strength of the tuberculin injected, the examination and preparation of the skin before injection, the depth of the injection, the hours of examination, etc., all are factors of importance. The approval of the intradermal tuberculin test by the Association as.one of the means of detecting tuberculous animals is recommended. The code for recording and reporting the results obtained by testing cattle intradermically with tuberculin, adopted by the United States Livestock Sanitary Association, should also be adopted by this Asso- ciation, and it is recommended that this action be taken. Though 366 Proceepines A. V. M. A. some objections have been made against the code, it is questionable whether they have sufficient weight to justify the confusion which would result from the adoption of different codes by the two most important American associations concerned with the protection of animals against infectious diseases. Another recommendation made by the Committee on Tuberculosis at the last annual meeting is a revision of the repert of the Interna- tional Commission on the Control of Bovine Tuberculosis published in 1910 and the preparation of a primer on bovine tuberculosis. Whether this recommendation was to be construed as imposing a task on the committee of this year is a matter about which its members are not in full accord. The work, however, has not been undertaken, and if it is the intention of the Association to have it done it is rec- omnia that a special revision and primer committee be appointed to do it. Recommendations were also made by the committee of last year regarding so-called no-lesion tuberculin reactors. It is with consider- able satisfaction that something can be said on this subject which may prove of interest. During the year ending June 30, 1921, the Pathological Division of the Federal Bureau of Animal Industry ex- amined material obtained from 1,296 cases of no-lesion reactors, and found acid-fast bacilli in all respects like tubercle germs, in 258, or virtually 20 per cent. The material examined was mostly congested lymph glands and glands showing small hemorrhagic areas. Such glands are not uncommon in nontuberculous slaughtered animals, hence the significance of the proportion of cases in which tubercle bacilli were found should not be taken as proving that only one-fifth of the no-lesion cases have beeen satisfactorily explained, but rather as evidence that tends to justify the belief that so-called no-lesion pears, with possibly rare exceptions, are infected with tubercle acilli. The insidious, chronic character of tuberculosis should naturally prompt us to draw the conclusion that a delicate, highly efficient diagnostic agent like tuberculin would detect a fairly large number of cases before the lesions had become gross enough to be visible to the unaided eye. Consider how difficult it is to select material from no-lesion tuber- culin reactors for microscopic study. A small fragment of tissue suf- fices for many preparations of the kind that can be examined under the high magnification required for the detection of tubercle bacilli, and the fragment, less than a grain, is part of an animal that rarely weighs less than several hundred and often more than a thousand pounds. To have proved, actually proved, that 20 per cent of 1,296 no-lesion tuberculin reacting cattle were actually infected with tuber- cle bacilli, if the difficulties in the way of obtaining the proof are duly valued, is either an astonishing technical achievement or the strongest kind of evidence to support the conclusion that most so-cailed no-lesion reactors are infected with tubercle bacilli and must be looked upon as dangerous animals that may sooner or later become spreaders of tuberculosis. Those who are inclined to doubt this interpretation should go to friends or acquaintances who have hunted tubercle bacilli in recognizable tuberculous lesions, and learn from them that their discovery in even such lesions too often is an eye-wearing, tedious, time-consuming task. It may be interesting to say here that sensitization to tuberculin can be induced in cattle and small experiment animals by injecting them with dead tubercle bacilli, and that dead tubercle bacilli until recently were of not rare occurrence in tuberculin. Whether their abundance occasionally was sufficient to sensitize cattle that received large subcutaneous injections of tuberculin has not been determined. ee — Pw Ae . Be aaa OAD. SER ie Pel eee) lt oT ee ed ee ae ee ¥ rm % Proceepines A. V. M. A. 367 Cattle can be sensitized also by drenching them with human tubercle bacilli, or by injecting them with lesions caused in guinea-pigs by human tubercle‘bacilli. Such bacilli, as is now well known, do not cause an active or a progressive tuberculosis in cattle. What the value of these facts may be in accounting for some no-lesion reactors can not be answered until further studies have been made. The question has been asked whether an animal may be sensitive to tuberculin as the result of previous injections. The answer to this is that though many tests have been made with different species of animals, no one has succeeded in showing that injections of tuberculin sensitize an animal to subesequent injections. As we well know, one of the disadvantages of the subcutaneous use of tuberculin is, wholly contrary to the sensitization of nontuberculous animals to subsequent injections, that it too often leads to the desen- sitization of tuberculous animals. Such desensitization, unless some means can be devised to give the slogan, “Once a reactor, always tuberculous” practical value, makes it possible for dangerous tuber- culous animals to remain unrestrained and unrecognized and to serve as unsuspected disseminators of tubercle bacilli. The Association, or a committee appointed by the Association, should give this matter careful study, and seek means through which it can be determined with reasonable certainty when an animal is examined whether it has at any time reacted with tuberculin. The suggested committee, if appointed,—and its appointment is hereby recommended,—should not be the Committee on Tuberculosis, and the men selected for it should be well informed on practical methods of marking animals and should know how to obtain the support of livestock interests to fur- ther the use of a workable method for identifying dangerous animals. Another resolution in the report of the committee of last year,— a report, by the way, that imposed a good many large tasks—recom- mended that a study should be made of the causes of the recurrence of tuberculosis in cleaned herds. Such recurrence is a subject on which probably any member of the committee could present a long dissertation, which, however, as it would contain little or nothing new, would be tedious rather than instructive. Two things are very important; one, that we should guard clean and cleaned herds against cattle that have become tolerant to tuberculin; and, two, that we should carefully study the cleaned herds under our supervision rela- tive to the simple, discoverable means through which they may be reinfected. That a stream of water, for instance, may carry tubercle bacilli a considerable distance from an infected to a clean or a cleaned herd and lead to the development or recurrence of tuberculosis is a fact that has been experimentally proved. That flies, rats, mice, birds, currents of air, etc., serve as agents to spread tuberculosis has not been proved, though definite investigations to incriminate them have been made. It has been proved that flies that live in a tuberculous environment may harbor tubercle bacilli in and on their bodies, and that the same is true of rats, mice and birds which have ingested tubercle bacilli or tuberculous tissues; but if these were im- portant causes for the spread of tuberculosis among cattle, it would not have been possible, as has been done at various places, to main- tain actively tuberculous cattle on a small area with nontuberculous, without the spread of the disease from the former to the latter. The Committee on Tuberculosis, which is clothed with the dignity reflected from the inclusion of a wholly superfluous adjective, “Inter- national,” in its name, owes it existence to a recommendation made by the “International Commission on the Control of Bovine Tuber- culosis” appointed by the Association in the year 1909. One of the principal functions of the committee, defined in the recommendation which called it into existence, was to report to the Association from 368 Proceepines A. V. M. A. time to time the discoveries made by investigators engaged in the study of tuberculosis. It therefore seems pertinent and desirable that a few words should be said, before closing this report, about the claims made in the course of the year by Calmette and Guérin in France and Nathan Raw in England that they have succeeded in pro- ducing attenuated strains of tubercle bacilli which may prove, or are alleged to have proved, serviceable for use as immunizing and thera- peutic agents in the fight against tuberculosis. As early as the years 1894 and 1896, attention was called to an originally virulent strain of the tubercle bacillus which had be- come attenuated through growth on artificial culture media, and which was proved to induce a measurable degree of resistance against tuberculosis in guinea-pigs injected with it and subsequently injected with virulent tubercle bacilli. A number of years later, as you will remember, we passed through the period of so-called bovo-vaccination against tuberculosis. Bovo-vaccination may be likened to the use of attenuated tubercle ba- cilli to obtain immunity against more virulent strains, as it required the injection of cattle with bacilli of the human type, against which they are normally resistant, to protect them against the bovine type, for which they are highly susceptible. More recently we were subjected to the Friedman turtle-bacillus fiasco. The turtle germ, for which specific therapeutic qualities of an antituberculous character were claimed, was nothing more nor less, so far as we know, than a bacillus of the human type of very low pathogenic virulence which had been isolated from a turtle that had been exposed in a zoological garden to the expectorations of a human tuberculous individual. And, incidentally, it may be worth mention- ing, after years of study and observation, a hot quarrel is still in progress in Europe concerning the value of Friedman’s turtle ba- cillus as an agent for the treatment of tuberculosis. The object of referring to this earlier work is to call to your minds that the use of attenuated, avirulent and aberrant or unusual strains of tubercle bacilli as immunizing and therapeutic agents is by no means new, and to justify the opinion that the claims made by Cal- mette and Guérin and by Raw, notwithstanding that these are men of the highest rank in the field of tuberculosis research, must not be lightly accepted as a final solution of our tuberculosis problems. When we realize that tubercle bacilli may lie dormant, without los- ing their virulence or changing their character, for months and years in the bodies of animals for which they are not truly pathogenic, we may say: In Europe, if live tubercle bacilli can be used with a fair degree of safety to protect cattle against tuberculosis, because of the great frequency of tuberculosis among European cattle, it may be a reasonable expedient to use them. But in America, with its much smaller proportion of tuberculous cattle, it is to be hoped that we will resort to no method of control or eradication that requires the injection of living tubercle bacilli of any kind, type, degree of viru- ence or degree of attentuation, at least not until the injection of such bacilli has been proved by searching, exhaustive experimental studies to be, on the one hand, harmless, and, on the other, truly and econom- ically beneficial. In America our aim should be total eradication, and no better path to this end has been defined than that along which our cooperative work’ between the Federal and State Governments is moving. E. C. SCHROEDER, Chairman. CHARLES E. COTTON, CARL W. GAY, M. H. REYNOLDS, FREDERICK TORRANCE. Proceepines A. V. M. A. 369 PRESIDENT WHITE: We have just heard a very valuable re- port from the International Committee on Bovine Tuberculosis. What shall be done with it? Dr. KrerNAN: I am in favor of the resolution and propose to vote for it. I merely rise to make the observation that I no- tice that less than half a dozen replies to the questionnaire were returned to the committee. I don’t like to have the impres- sion go out that that indicates the sentiment of the veterinarians on the tuberculin test. From October 1 to June 30, 1921, there were tuberculin tested by the subcutaneous method 251,000 cat- tle, of which 10,000 animals reacted; by the intradermic test 648,697 head of cattle, of which 24,554 reacted to the tuberculin test. The percentage of reactors by relative tests was relatively the same, indicating that the intradermic test is used quite gen- erally throughout the United States. I hold no brief for either one. These are cold facts and figures for the work being done. Another observation I wish to make is with regard to lesions. The report of July, 1921, shows that of 137,000 head of cattle tubereulin tested in cooperative work, 302 animals were found to disclose no microscopic lesions, representing 6.57 per cent of those showing no lesions. I think that will compare favorably with all the data that have been gathered annually from the beginning. I move that the resolution be accepted. (The motion was duly seconded and carried. ) AMENDMENT TO By-Laws PRESIDENT WHITE: Is there any unfinished business? ‘SEcRETARY Mayo: There is a minor change in the By-Laws that was proposed at Columbus last year. It was to change Article I of the By-Laws on the order of business, placing the reports of officers as No. 7, before the election of officers, instead of afterwards as it is at present. We have not followed this plan, but it is a minor change. PRESENT WHITE: You have heard the statement of the Sec- retary in regard to this minor change in the By-Laws. What is your pleasure in regard to it? Dr. Corton: I move its adoption: (The motion was seconded and carried. ) SPECIAL COMMITTEE ON PREVENTION AND CONTROL OF ANIMAL DISEASES PRESIDENT WHITE: Is there any new business? Dr. Cary: I move that a special committee of not more than five members be appointed by the President to report at the next meeting of the A. V. M. A. on ‘*The Prevention and Control of Animal Diseases,’’ along the lines suggested by Dr. Munee in 370 Proceepines A. V. M. A. his paper on that subject before the Section on Sanitary Science and Police. (The motion was seconded and earried. ) Puace or Next MEETING Dr. ExiAson: Would it be appropriate at this time to extend an invitation to the Association for next year’s meeting? PRESIDENT WHITE: I presume it would not be out of order. Dr. Ferauson: Last July, at the meeting of the Wisconsin Veterinary Medical Association, I was delegated to extend you an invitation to meet at Madison, Wisconsin, next year. It is needless for me to describe the advantages that you will have in meeting in Wisconsin. Nearly all of you are familiar with that State. The only question that might come up in your minds is as to having facilities to house you there, and I can assure you we have ample facilities to take care of every member and visitor if you decide to meet there. We can put up a program that will be of special interest to the practicising veterinarians. As you all know, Wisconsin is the leading dairy State in the Union, and Madison is so located that you may have the opportunity to look over some of the best bred and some of the best individual dairy cattle in the world. Dr. West, one of the local men in Madison, is famous for getting together a clinic. I can assure you that if you meet there no one will be dissatisfied with the size and the quality of the clinic that we can give you. In behalf of the Wisconsin Veterinary Medical Association I extend the invitation to meet with us next year. (Applause.) PRESIDENT WHITE: I do not believe it would be wise at this time to commit ourselves finally in regard to our next place of meeting. Personally, I am very much in favor of what the last speaker said, but we will receive invitations from other parts of the country and it would hardly be fair to them not to allow them to be heard. I understand that all of the representatives have not yet arrived. However, use your own pleasure in the matter and not mine. Dr. Ev1Ason: Just in further invitation from the city of Madi- son, and on behalf of the Board of Commerce and the Department of Agriculture, of which I am a part, we extend to you cordially an invitation to visit the city of Madison. We have one of the most splendid capitals in America, and we are so situated that we can give you a good time. Some questions have arisen as to whether we are equipped with hotel facilities. They are not elaborate, but I assure you that you will be taken care of. We have made arrangements so that we know just where we ean be if you come. Every room in Madison has a bath. All you have to do is to bring your bathing suit. You can step right off into the lake. We have four lakes right in proximity to Madison. If you want to fish in the morning or in the evening facilities will EE My es Hh aeriened 5 hing icp has ; r aM r 4 | . . | a Proceepines A. V. M. A. 371 be provided so you may. I also extend, on behalf of the Gov- ernor, an invitation to visit our State. Dr. Cary: I move that this question be laid on the table until all the invitations are in. Dr. JENSEN: Could they not be presented at this time? PRESIDENT WHITE: They are not all here. Dr. Fercuson: Mr. President, it was not my intention to have this invitation accepted at this time. I wanted to get it before the members while they were all present. Dr. Srance: I think perhaps an amendment would not be out of order. It seems to me the proper way to handle this would be to refer this invitation, together with any others that may be received, to the Executive Board. I move that this invitation, together with any others that may be received, be referred to the Executive Board for their action. (The motion was seconded by Dr. Cary.) PRESIDENT WHITE: Dr. Cary’s motion to lay this on the table was not seconded, so Dr. Stange’s motion holds. Dr. Koen: I believe that Dr. Stange speaks correctly when he says that the meeting place shall be decided upon by the Executive Board, but I believe that it has been the custom in previous years to give the Association an opportunity to express its preference. I don’t think that it is fair to those who are here or to the people that have invited us to Madison and Wis- consin to refer this invitation to the Executive Board without an opportunity of the members assembled here to express their preference. If this motion can be so amended that this Associa- tion or convention shall have an opportunity, before it adjourns, to express its preference in the matter, then I am satisfied to let it go over; but I don’t believe the motion should carry to dispose of Madison finally by referring it definitely to the Executive Board. I hope that the question will be voted down. Dr. Cary: I would like to call for the reading of the Constitu- tion and By-Laws on this question. (Secretary Mayo read from the Constitution and By-Laws governing meetings of the Association. ) Dr. JENSEN: I am mighty happy that for once I can agree with Dr. Koen. This idea of everything being referred to the Executive Board reminds me of the fact that somebody called it the executing board. I believe we should have a little voice in this matter. Dr. KieRNAN: Since 1910 when this question was discussed in New York as to whether the date and place of the meeting should be fixed by the Executive Board or the membership the question has never arisen, but the Association has the perfect right to fix the place for holding the meeting. As I understand this, it is not for the Executive Board to dispose of the matter, but to give opportunity to others to extend invitations to hold the meeting 372 PROcEEDINGS A. V. M. A. next year. There are other places that have in mind extending an invitation to this Association, but they understand it is handled by the Executive Board. Wisconsin has an equal oppor- tunity with any other city or State before the Executive Board. I think that is fair enough. Dr. Koen: All these other towns have had an equal chance with Wisconsin to extend an invitation. I am not asking at this time that the other cities that want to invite us be not given an opportunity, but I do ask that Dr. Stange’s motion be voted down in order that the convention may have opportunity to voice its preference as to where we shall meet. If Dr. Stange’s motion carries, this Association shall have no voice in the matter what- ever, but all the other invitations that come will be referred to the Executive Board. Dr. Cary: I move we postpone this until all the invitations arrive. (The motion was seconded. ) PRESIDENT WHITE: Is that an amendment to the motion or a substitute motion ? - Dr. Cary: I move that this question under discussion be post- poned until all the invitations have arrived. I suppose it is an amendment. Dr. JENSEN: I would like to see embodied in that motion a provision that this be decided by the Association and not by the Board. ; PRESIDENT WHITE: The motion comes on the amendment by Dr. Cary. (The amendment was carried. ) PRESIDENT WHITE: Now we will vote on Dr. Stange’s motion as amended. (The motion as amended was carried.) Adjournment. NEW COMMITTEES OF THE A. V. M. A. The following appointments have been made by President A. T. Kinsley: Committee on Legislation—J. G. Ferneyhough. Committee on Resolutions —D. M. Campbell, chairman; C. H. Stange, V. A. Moore, Geo. H. Glover, R. R. Dykstra. Audit Committee—R. P. Marsteller, chairman; O. J. Kron, G. A. Scott, A. Lockhart, J. V. Lacroix. Committee on Necrology—H. KE. Bemis, chairman; J. W. Con- naway, A. H. Baker, W. B. Craig, G. A. Johnston. Committee on History. J. W. Adams, chairman; R. C. Moore, G. H. Berns, T. H. Edwards, C. D. MeGilvray. ASSOCIATION NEWS ee Committee on Anatomical Nomenclature.—S. Sisson, chair- man; H. 8. Murphey, Mark Francis, Earl Sunderville, F. W. Chamberlain. International Committee on Bovine Tuberculosis —J. A. Kier- nan, chairman; Luis Santa Maria, F. Torrance, C. E. Cotton, W. T. Spencer. Committee .on. Abortion.—C. P.. Fitch, chairman; E. C. Schroeder, F. M. Hayes, A. Eichhorn, T. H. Ferguson. Committee on International Veterinary Conference—J. R. Mohler, chairman; A. Eichhorn, L. A. Meritlat, David S. White, V. A. Moore, F. Torranee. : Salmon Memorial Committee.—S. Brenton, chairman; H. Pres- ton Hoskins, J. R. Mohler, J. G. Rutherford, H. M. Graefe. Inautard Memorial Committee —J. F. DeVine, chairman; W. H. Lowe, W. G. Hollingworth, A. D. Knowles, W. H. Dalrymple. Committee on Emblem.—sS. E. Bennett, chairman; R. F. Bourne, J. F. McKenna. Committee on Prevention and Control of Anima! Diseases.— H. W. Turner, chairman; L. G. Cloud, U. G. Houck, G. H. Hart, I. E. Newsom. Representatives on Revision of Narcotic Law. chairman; R. R. Clark. Representative on Board of Managers, Horse Association of America.—Geo. A. Dick. Representative American Research Council Committee.—L. W. Goss. J. Ey Turner, NEW MEMBERS Membership cards have been sent to all the newly elected members together with an application blank and a letter urging them to make a special effort to get a new member. With their enthusiasm for the A. V. M. A. we are expecting good returns. How many members will make a personal effort to get one vet- erinarian. to join our association? It would double our mem- bership. The Secretary has written to some English veterinary jour- nals calling attention to the advantages of joming the A. V. M. A., and it is hoped that some of our confreres on the other side will respond. The Secretary has received a letter from Dr. Gabriel Malda, Seeretary of Public Health of Mexico, thanking the A. V. M. A. for courtesies extended to Dr. Luis Santa Maria, the official delegate from the Republic of Mexico to the Denver meeting. Dr. Santa Maria joined the A. V. M. A. at that meeting. An application for membership has been received from Dr. 374 ASSOCIATION NEWS J. E. Aghion Bey, Principal Veterinary Officer, State Domains, Sakha, Egypt. He is a graduate of the Ontario Veterinary College in 1907. VETERINARY ASSOCIATION OF MANITOBA A special meeting of the members of the Veterinary Associa- tion of Manitoba was held in the Royal Alexandra Hotel, Winni- peg, Tuesday evening, September 27, 1921, for the purpose of discussing questions of importance to the livestock industry and also Dominion-wide registration of veterinary surgeons in the Dominion of Canada, and for the appointment of a delegate to attend a conference of veterinary surgeons to be held in Ottawa in November. The chair was occupied by the President, Dr. F. W. Bryant, and the following members were present: W. J. Hinman, R. A. McLoughry, W. A. Shoults, W. Hilton, J. A. Stevenson, J. A. Munn, H. R. McEwen, N. V. James, H. Bradshaw, A. L. Alton, G. B. Miller, G. A. Bowman, W. A. Hilliard, J. G. MacDonald, H. C. Storey, H. Colebourn, J. W. Smith, S. H. Kesten and J. B. Still. A lengthy discussion took place regarding matters of im- portance to the livestock industry and the Dominion-wide reg- istration of veterinary surgeons. It was moved by Dr- Hin- man, seconded by Dr. Bradshaw, that a committee composed of Dr McLoughry, Dr. Shoults and Dr. Bryant draft a reso- lution embodying the sense of the feeling of the meeting for the guidance of the association’s delegate who was to-be de- tailed to attend a conference of veterinary surgeons to be held in Ottawa during the month of November. This motion ecar- ried, and the meeting then took a recess while the committee drafted the resolution. On reassembly the resolution was pre- sented, and on being put to a vote it was ¢arried. Dr. J. A. Munn of Carman, who was elected as the Associa- tion’s representative on the Dominion Advisory Board on Vet- -erinary Education at the last annual meeting, was asked to rep- resent the Association as their delegate and was furnished with the foregoing motion for his guidance. Dr. Munn assured the members that he would comply with the resolution and do everything possible in the best interests of the veterinary asso- ciation and the profession at large. ASSOCIATION NEWS 375 On motion of Dr. McEwen, seconded by Dr. Stevenson, the President, Dr. F. W. Bryant was selected as an alternative dele- gate, in the event of anything arising to prevent Dr. Munn from attending the conference. J.B. Stiuu, Secretary. CALIFORNIA STATE BOARD OF EXAMINERS The State Board of Examiners of California held a meeting in Los Angeles on October 20, at which time a class of eight took the examination, four of whom passed. The four passing were graduates of the following colleges: Two from Washington State College, one from Chicago Veter- inary College, one from Indiana Veterinary College. Of those failing one was from McKillip Veterinary College, one from Chicago Veterinary College, one from Kansas City Veterinary College, one from. Western Veterinary College. JOHN L. Tyuer, Secretary. CENTRAL MICHIGAN VETERINARY ASSOCIATION Tuberculin tests in cattle formed the subject of talks by sev- eral speakers at the meeting of the Central Michigan Veterinary Medical Association, held at the Jackson City Club on October 14. The meeting was one of the most successful and profitable ever held by the Association. Approximately 50 members were in attendance. Dr. B. F. Killham, State Veterinarian, spoke on the work of the new State agricultural department, and the methods used in dealing with bovine tuberculosis. Dr. J. A. LaCroix, Chicago, editor of the North American Veterinarian, spoke along similar lines, while Dr. C. C. Mix, of Battle Creek, took for his subject the economic value of tuberculin testing. Dr. C. C. Clement, of Hudson, gave a synopsis of the early days in tuberculin testing, and drew a verbal picture of the re- markable progress which has been made. The speaker was one of the charter members of the Michigan Veterinary Association, which was organized in 1883. The progress of the State As- sociation was outlined by Dr. Rummels, of Lansing. He re- ported that 48 applications for membership had been received during the past year. Centralized killing of livestock was advocated as an economic 376 ASSOCIATION NEWS necessity by Dr. H. H. Sparhawk, of the Detroit Board of Health. He stated that the State metropolis has 100 abattoirs under inspection, and that all meat was inspected and stamped before entering the city. He declared that 2,000 pounds of veal had recently been condemned because the calves had been slaughtered before they were two days old. That the pronounced increase in hog cholera in the State is due to improper vaccination and use of virus was the opinion expressed by Dr. Newton, of Lansing. Other speakers were Drs. A. L. Curtis, of Hillsdale; A. Z. Nichols, Pittsford; and R. S. Johnston, bacteriologist of the State Board of Health. Dr. H. L. Roberts, meat and milk inspector in Jackson, acted as toastmaster at the banquet which followed the discussions. W. N. ArMstrRONG, Secretary. PHILADELPHIA VETERINARY CLUB The reguiar monthly meeting of the Philadelphia Veterinary Club was held on Tuesday evening, October 25, at the Veteri- nary School, Philadelphia, Pa. Dr. J. A. Kolmer, Laboratory of Dermatologic Research, University of Pennsylvania, addressed the Laboratory Section on ‘‘Some Phases of the Complement Fixation Test.’’ The regular club meeting was an open meeting. No special subject was on the program. Several of the members reported on cases that they had treated, which called forth interesting discussions, and much valuable information was obtained in general. | We had an exceptionally large attendance. C. S. RockweE.i, Secretary. CALIFORNIA STATE VETERINARY MEDICAL ASSOCIATION The next meeting of the California State Veterinary Medical Association will be held at Davis, California, from January 2 to the 7th, inclusive, 1922. This will be a practitioners’ short course held at the College of Agriculture of the University of California and it is expected to be even more successful than any held heretofore. | | | oe y Sie ie a cae | ASSOCIATION NEws 37 Particular stress will be laid this year on diseases of cattle. Dr. D. H. Udall of the Cornell Veterinary School will be the principal lecturer on this subject. Other prominent men who will deliver lectures during the Short Course are: Thomas F. Hunt, Dean of the College of Agriculture; Elwood Mead, Professor of Rural Institutions; B. H. Rawl, formerly Assistant Chief of the United States Bureau of Animal Industry; G. H. Hecke, State Commissioner of Agriculture; J. P. Iverson, Chief of the State Bureau of Animal Industry, and others. JosEPH M. Arspurua, Secretary. PENNSYLVANIA STATE VETERINARY MEDICAL ASSOCIATION The Board of Trustees of the Pennsylvania State Veterinary Medical Association has authorized the announcement that the 1922 meeting will be held in Harrisburg, Penna., Tuesday and Wednesday, January 24th and 25th. Every effort is being made to have the 1922 meeting as attrac- tive to practitioners as the 1921 meeting proved to be. The as- sistance of the practitioners of Pennsylvania, who have organ- ized local Veterinary Clubs throughout the State, is being en- listed. At the present time almost every veterinarian in the State belongs to one of the eight clubs now meeting regularly. Each Veterinary Club has been asked to select one of its mem- bers to present.a paper of interest to the practitioner and two additional men to open the discussion of the paper. Many of the clubs have notified the Secretary that their men have been se- lected and keen rivalry for the presentation of the best paper in- sures an excellent program. The Pennsylvania State Veterinary Medical. Association has, for the past few years, been a member of the Allied Agricultural Associations. i. ie eee will make the following improvements or changes in the order enumerated below: (For remarks use other side) In regard to hog cholera, more attention has been given to its control, through the use of serum and virus, than to its pre- vention. The swine owners of the country have been led to be- lieve that liberal use of anti-hog-cholera serum and hog-cholera virus is all that is necessary for hog cholera prevention. | = 410 T. EK. Munce Until matters that have to do with prevention are recognized as beimg of major importance, we can not hope to make satis- factory progress in permanently eradicating any disease. Destroy the breeding places of flies and mosquitoes and those pests will become extinct. | Afford protection to herds and flocks that are now free from disease and there will be no disease to eradicate. Instead of giving a list of conclusions we will give a few prac- tical examples of disease prevention as they are being practiced in Pennsylvania today. I wish to say first in this connection that it is possible, and we believe practicable, to prevent every disease of a transmissible character in animals. Many years ago in one of our large cities, when street cars were drawn by horses, one of our veterinarians was called to give advice on the treatment of sore shoulders which were causing disability in more than 50 per cent of the horses. He not only advised but insisted that the collars and hames be properly fitted, the manes kept smooth and the shoulders clean. His ad- vice was followed, with excellent results, the percentage of sore shoulders being reduced to less than 10 per cent. One of our leading breeders started with twelve head of cat- tle, and from this foundation, with proper veterinary advice, has built up a large herd. He adopted the motto, ‘A cow that leaves the farm must not return.’’ During the ten years of his breeding experience there has not been a case of infectious dis- ease in his herd. That was prevention. The same methods have been successful in other herds of more recent origin. Another breeder went along for a period of ten years, build- iig up a splendid Guernsey herd without the appearance of any infectious disease. He then sought new blood, without proper advice, and unknowingly introduced abortion to his premises. Three months later the disease was recognized. The newly ac- quired cattle were isolated and the premises thoroughly dis- infected. The abortions ceased and there has been no return up to this time. Another herd of over 150 head of cattle may be cited, in which nearly one year ago abortion was in progress. Up to that time six or seven cows had aborted. Preventive measures were en- forced, and in addition to those that had aborted eight other cows were taken from the herd, and not a single case of abortion PREVENTIVE MEASURES AGAINST ANIMAL DISEASES 411 has occurred in the herd since, although one of the isolated cows aborted about three weeks later. In another herd of over 200 head, for the past ten years, with the exception of the last three, a large number of eases of abortion and calf scours occurred each year, and in that herd probably 20 per cent of the older cows would react at the present time to serological tests for abortion; but for the past three years there has not been a single case of abortion. Each pregnant cow was placed in a specially constructed stable near ealying time and not returned to the breeding herd until after her genital tract was free from discharge and thoroughly clean. The calving stall was then effectively fumigated and made ready for the next cow. Several breeders are working a plan similar to this at the present time. These same premises at one time were badly infected with tuberculosis, and after it had been eradicated preventive meas- urs were put into operation, and there has been no return of tuberculosis for at least four years. In this same herd calf scours was successfully prevented by giving the dam a clean place at parturition time and keeping the calf in a clean place for at least one week after birth. Calf scours has been prevented on several farms in the same way. We know of several instances where it seemed impossible to raise any calves, but when the above practice is put into opera- tion no losses from white scours occur. White scours is success- fully and practically prevented by giving the dam a cleaned and fumigated stall at parturition time, the calf being kept in that clean stall or another similarly cleaned one for about one week after birth. _ A farm devoted to the raising of purebred hogs, said to be the largest of its kind, was kept free from transmissible swine dis- eases, though situated in a district where cholera was prevalent. This was accomplished by preventive measures, such as thirty days’ isolation of newly purchased swine, proper arrangement and sanitary equipment of buildings and pastures, careful in- quiry into the sources of purchased feeds, frequent observance of the physical condition of each member of the herds, and the applying of approved methods of feeding and breeding. It has been demonstrated on numerous occasions that proper housing, ventilation and management are the greatest measures in the prevention and repression of poultry diseases. 419 T. E. Muncre Johne's disease of cattle is becoming of serious importance in some herds, and in our State when it has once gained foothold it is the most baffling of all diseases. Fortunately not many herds are afflicted. The best that we can recommend is that breeders should be very careful not to buy from affected herds. At the 1913 meeting of this Association it was the unanimous opinion that the public watering trough was one of the most important factors in the transmission of glanders and the in- dividual drinking cup one of the best methods to prevent trans- mission of that disease. On numerous occasions it has been proved that those opinions were a stimulus to the installation of proper preventive measures. We suggest, in order to bring about more interest and to stimulate more enthusiastic action in this subject, that the fol- lowing be given consideration and study by members of this Association : 1. Let prevention of disease, rather than disease itself, act as the medium that brings the animal husbandman and the veteri- narian together. 2. Closer unity of Federal and State officials dealing with animal diseases, with the various health and educational depart- ments, physicians, hospitals, medical and agricultural colleges and other institutions of learning. 3. Veterinary and agricultural colleges could do more toward disease prevention if stress were placed on this subject during the courses of instruction. 4. There should be a better understanding by the people of the intercommunicability of diseases and the relation of animal diseases to public health. 5. Federal, State, municipal and practicing veterinarians should become better acquainted with, recommend and practice preventive methods. 6. Areas now free from disease may be kept free if the owners of livestock are aware of the danger of purchasing from outside areas and are familiar with the necessary precautions against disease. 7. Federal and State officials should give more attention to maintaining the health of animals on premises where there is no reason to believe transmissible disease exists. 8. A system of identifying animals whereby they may be traced to previous owners. 9. Closer cooperation of State, Federal and municipal agents, PREVENTIVE MEASURES AGAINST ANIMAL DisEASES 413 boards of health and butchering establishments with the view of finding the source of diseased animals. 10. The vendor of a diseased animal, sold for food purposes, should be liable for the purchase price when such animal is con- demned in the hands of the butcher. In this connection special legislation would be helpful. 11. Legislation requiring a tuberculin test of cattle and mal- lein test.of horses offered at public sales. 12. Strict legislation on the spread of hog cholera and bovine abortion and other transmissible diseases through public sales. 13. Poultry and sheep diseases cause large losses which could be materially reduced if our profession were to give more atten- tion to their prevention. 14. Much could be done by quarantine, sanitary and disin- feeting measures in preventing influenza in horses and other dis- eases incidental to shipping, distemper in dogs, ete. 15. Disinfecting of stockyards and stock cars. 16. The value of more publicity through agricultural and daily press and county farm bureau organizations. We recommend that this Association appoint a Committee on Disease Prevention to consider the above suggestions and make additions; that the committee report at the next meeting of this Association. Should not the members of this, the greatest of all associations of its kind, take advantage of the above examples, which were made possible by some of our outstanding, wide-awake and think- ing stockmen, who, by employing the fundamental principles of prevention, have gone a step beyond us? Should we not take their examples as our fundamentals and formulate methods which will do for others what these men have done? We firmly believe that preventive measures are equally im- portant as detecting and removing diseased animals. We wish that more could see and appreciate the importance of prevention. We are thoroughly optimistic of the future and we believe that it holds much in store for our profession and stockmen. Our appeal is that veterinarians and stockmen unite more closely than ever before; that we think and work in unison as smoothly as the swinging of a pendulum, to the end that we can spend at least half of our time and knowledge in devising better methods of disease prevention rather than in concentrating all our agen- ‘cies upon one line. Until that is done, we veterinarians will not be in position to act in capacity of expert advisers. 414 T. E. Munce Veterinarians have a tremendous responsibility. Are we ex- erting ourselves to the fullest extent to meet this responsibility adequately ? If we are to merit the continued confidence of stockmen and their organizations as expert advisers, we must be able to do in- finitely more than to diagnose and treat disease. Stockmen are equally concerned in knowing how to prevent disease. DISCUSSION Dr. MAuRICE C. HALL: The paper which I will present is along the same line as Dr. Munce’s paper, and I am under the impression that it does not entirely agree with some of the statements. As you all know, however, when a paper is read to you it is very easy to mis- understand it or to get a false impression, and I am not sure that I have understood Dr. Munce correctly. However, I think he took the position that prophylaxis was always feasible in the transmission of a disease, and if my understanding was correct, I can hardly agree with that statement. I think you all recall that during the “flu” epidemic during the war a great many elaborate precautions of a prophylactic nature were taken, to no avail. You will recall that in connection with yellow fever, before it was known that the mos- quito was the transmitter of the disease, elaborate prophylactic meas- ures were used. It is possible to figure out prophylactic measures even where a life history is not well known, but under those condi- tions the measures are not usually feasible or practical, and there- fore not valuable. Dr. J. A. KIERNAN: I am in thorough accord with all the sentiments expressed in Dr. Munce’s most admirable paper. I rise only to call attention to a reference he made to comments from livestock owners respecting the accredited-herd plan. My understanding of that com- ment was that animals added to accredited herds are permitted to be introduced and fully accredited after being subjected to two tuberculin tests sixty days apart. From the very inception of the accredited-herd plan it. was recog- nized that it was not 100 per cent perfect, but it had to be made a practical operating plan that would fit into the business of raising livestock. It is true that animals after two tests may be added to herds, but that it works out in a practical way F want to demonstrate by data obtained in the retesting of the 8,000 accredited herds that are upon the list. Some inquiries were made as to the number of accredited herds removed from the list during the year. These data cover the fiscal year terminating June 30, 1921. It is true that not all of the 8,000 herds on the accredited list were subjected to a retest, but upwards of 3,000 of those herds were. There were removed from the accredited list during that time 87 herds. Eighty-seven herds of animals that at a previous test had passed the tuberculin test upon a second test were found tuberculous and were removed. For other causes there were removed other animals; for instance, animals exposed to infection at shows, at livestock expositions; 27 herds were taken off the list for that reason. Of the 87 herds, the total number, there were but 33 herds containing more than 2 ani- mals and 14 herds that contained more than 3 animals. I think any kind of a regulation or any kind of a business that has so few errors and so few omissions in it, as has this accredited-herd plan, can stand such criticism as has been made by the owners. As I say, it is a practical proposition and was made for the practical owners. . | PREVENTIVE MEASURES AGAINST ANIMAL DISEASES 415 There was another matter mentioned in the paper. I don’t want to misquote Dr. Munce, but my understanding was that during the foot-and-mouth disease outbreak complaint was made that the infec- tion was spread by veterinarians and inspectors. I don’t know how that was in some territories, but I had the privilege of working in three or four States and in McDonough County, Illinois, in which there was more infection than in any similar area in the United States, hav- ing more than 200 herds condemned in that area. We had occasion to trace the spreading from farm to farm in defense of that very accusation that inspectors and veterinarians were distributing the in- fection, and in not one single instance could we find, after an ex- haustive investigation, where a veterinarian or inspector had spread the disease. We traced the origin and spread of infection, and pre- sented the results at the conference on foot-and-mouth disease held in Chicago in December, 1914, when a number of livestock owners and lawyers were casting aspersions upon the veterinary profession that they were spreading the disease, and we outlined the facts and how the disease was spread. COMMISSIONER J. M. WHITTLESEY (Hartford, Conn.): Dr. Munce, I think, is portraying somewhat eastern conditions. I am from New England. I think Dr. Munce’s paper is one of great importance. I wish to call your attention to a few herds we have found in Con- necticut where evidently the great spread of tuberculosis was the fault of feeding and managing the cattle. We found some suffering from malnutrition. We found a very heavy percentage of tuberculosis in herds that were crowded to the limit of production and housed in barns the year around and forced during the war to produce the maximum amount of milk and that suffered physically. We got a tremendous spread in those herds. I presume that paper of Dr. Munce’s will interest those who are dealing with eastern conditions more than the western cattlemen, but it certainly is a very important subject to me. Dr. Cary: I think this is a “very valuable paper. I think one of the most valuable suggestions made by Dr. Munce was the suggestion that we have a committee appointed by this section or the general associa- tion to cover this subject and bring out the question of prevention of various conditions in various parts of our country. I think if it is in order a motion ought to be made to that effect and have a committee appointed. Whether it should be done before this section or the general house is another question, but I think that should not be overlooked. : CHAIRMAN JAKEMAN: Dr. Munce, do you wish to reply to the discussion? Dr. MuNcE: It was not my intention to reply. I did not intend to precipitate any discussion or any criticism of the accredited-herd plan, but I simply expressed as best I could the questions that were brought to our attention by some of our good stockmen. They asked this question, If it is safe to accredit cattle whose history in many cases is unknown, why isn’t it safe to accredit herds on two negative tests sixty days apart whose history is known? On the matter of spreading infection by foot-and-mouth disease, I simply tried to emphasize by that illustration how important it is that veterinarians—I wasn’t speaking of inspectors or any other branch of the veterinary profession—be exceedingly careful about carrying in- fection. We had in Pennsylvania several cases where foot-and-mouth disease was carried by careless veterinarians. I made the suggestion in reference to a committee because I felt that the subject was being neglected as it is, and it is of such tre- mendous importance that perhaps it might be well to consider that suggestion, if the Association sees fit, at the general session. GENITAL INFECTIONS IN THE BULL By Herspert L. GILMAN Department of Obstetrics and Research in the Diseases of Breeding Cattle, New York State Veterinary College, Cornell University, Ithaca, N. Y. THE STUDY of the infections of the genital organs of the bull, and the associated structural changes, offers practically a virgin field for intensely interesting research work. Vet- erinarians and breeders have long recognized the bull as a po- tential factor in the spread of genital infections in the herd, but even then he was usually looked upon as merely a mechani- cal carrier of the organisms. The fact that he might be an active spreader has been quite generally overlooked. Too fre- cuently the ability of a bull to copulate in an apparently normal manner is taken as conclusive evidence of his procreative powers. The question as to whether or not the semen contained norma! active spermatozoa, and whether the genital organs harbored organisms capable of being transmitted to the female during ejaculation of the semen, seems to have been entirely disregarded until comparatively recently. It has been demonstrated by Carpenter (1) that the genital organs of cattle harbor several species of organisms, other than the Bang bacillus, which interfere with reproduction. The question is, Does the genital tract of the bull become infected with these same organisms, and if so, are they eliminated with the semen and is the female infected during copulation? Like- wise, what gross and histological changes occur in his genitalia, and if so, what functional changes result therefrom? Clinical evidence clearly incriminates the bull in a large number of cases, and the clinical findings, substantiated by laboratory diagnoses, demonstrate that the bull often becomes a dangerous source of genital infections in the herd. Disregard for the part played by the male has by no means heen confined to the veterinarian, for the physician likewise has, until comparatively recent years, almost entirely neglected or failed to appreciate sterility in the male. His part also in the transmission of the general genital infections has been given little or no consideration. Careful and painstaking study of the subject has proven that the percentage of male sterility is quite 416 GENITAL INFECTIONS IN THE BULL 417 high, and that he plays a very important part in the spread of venereal infections other than those of a specific type. Even otherwise normal healthy men are occasionally encountered in whose semen no spermatozoa are found. Conditions are prac- tically identical in the bull as far as the work has been carried on. The genital organs of the bull are even more accessible to clinical examination than those of man, and his semen more easily obtained. ihe bull, when once infected, is a much more dangerous spreader than the cow, due to his intercourse with so many females. The cow may spread her infections merely to the bull or bulls with which she may copulate, while an infected bull naturally becomes a disseminator to as many females as he may be called upon to serve. It remains, therefore, for the veterinarian to consider the bull in the study of genital infec- tions in every herd which he is called upon to treat. Com- paratively too much attention has been given to the diseases of the cow, with practically no consideration of the source and mode of transmission of the infections. References to the part played by the bull in the spread of ‘‘eontagious abortion’’ as caused by the Bang bacillus are quite numerous. Bang (2) originally called attention to this fact, but came to no definite conclusions. Later MeFadyean and Stockman (3) attempted but failed to infect cows by using a soiled bull for service. Hadley and Lothe (4) state: ‘‘A large number of stockmen hold that the bull is an important factor in the transmission of contagious abortion in herds. A smaller number believe that the bull acts merely as a passive carrier of the abortion disease and is not actively concerned in the transmission.’’ Their attempts to infect abortion-free heifers with abortion-infected bulls were negative. Buck, Creech and Ladson (5) applied the agglutination test to 325 mature bulls, of which 288 were negative and 37 positive. Bacillus abortus was isolated from five animals, of which three showed marked lesions, two in the seminal vesicles, and one in the left testicle. They conclude: ‘‘B. abortus may involve organs of the generative apparatus of bulls, producing chronic inflammatory changes. Of the generative organs, the seminal vesicles appear to furnish the most favorable site for the lodge- ment and propagation of abortion infection.’’ Schroeder and Cotton (6) cite the ease of a bull which re- 418 Hersert L. GrMan acted to the abortion test, and on postmortem B. abortus was isolated from an abscess of one epididymis. They state: ‘‘Our attempts to produce a similar case of infection artificially failed ; and, in agreement with the difficulties many investigators have had to obtain incriminating evidence against bulls, we have thus far failed to infect bulls in any way that justifies the assump- tion that they are important factors in the dissemination of abortion disease.’’ Further, they conclude: ‘‘Regarding the dissemination of abortion disease by bulls we may say, however, that it would be foolhardy in the dim light of our present knowl- edge to take liberties with reacting bulls, or bulls from infected herds, or promiscuously used bulls.”’ The work so far discussed shows that B. abortus has ocea- sionally been isolated from the genitalia of bulls, and that they do at times react to the agglutination and complement-fixation tests with B. abortus antigen. Attempts at artificial inoculation by natural channels have failed, with the possible exception of McFadyean, Sheather and Minett (7), who were able to in- fect the bull by the prepuce in two cases and by mouth in one case. The results, however, are by no means conclusive, and the duration of the infection and transmission to the cow are not discussed. They conclude, nevertheless, that cattle of any age and either sex may be infected by natural channels with the bacillus of epizootic abortion. Proof of the transmission of B. abortus by the male to the female has at no time been brought forward, and even the possibility of his acting as a mechanical carrier is merely assumed. ; Hadley (8) states that unquestionably the male often becomes infected with germs that produce the various secondary dis- eases in the female, which are more properly classed under the more inclusive term of ‘‘abortion disease.’’ Further on he states that douching the bull before and after service certainly will keep the bull from transmitting the organisms that cause the secondary infections. One might infer from this that Hadley believes that the genitalia of the bull become infected with many varieties of organisms, and that they are transmitted to the female, producing disease in her reproductive canal. How- ever, he does not state just what the secondary infections are. Even though the internal genitalia did become infected, and the organisms were eliminated during ejaculation, no amount of douching would prevent the transmission of the infections. GENITAL INFECTIONS IN THE BULL 419 The work so far reviewed has been limited to infection with and the transmission of B. abortus, with no attention to or regard for other infective agents which have been proven to be inti- mately associated with diseases interfering with reproduction in the female. While this organism is a very vital factor in the problem of genital infections, the organisms usually referred to as ‘‘secondary invaders’’ are of primary importance and must never be overlooked if one is to get a clear understanding of the problem. Their role is by no means insignificant. The semen, until the work of W. W. Williams (9), received only slight attention in the study of sterility in cattle. Its nor- mal character has been simply taken for granted. In his work he calls attention to the importance of an examination of the semen in diagnosing sterility in the bull, giving methods for the collection of samples, staining, and some of the abnormalities encountered. The work is fundamental and should stimulate interest in this important method of diagnosis. Later he goes into a more extended discussion of the problem, concluding that the clinical examination is of vital importance. Of 40 bulls examined, he finds that 20, or 50 per cent, showed lessened fer- tility, and others aside from these showed minor changes in the genital organs or semen. W. L. Williams (10, 11) calls attention to the same fundamental problems, and in a subse- quent paper (12) states: ‘“Clinical studies now indicate with great clearness that the bull is an active spreader of that group of genital infections which cause sterility, abortion and related phenomena. ”’ Most of the present studies have been carried on in cooperation with the last two mentioned authors, in the hope of correlating the clinical and laboratory: findings. In order to bring out the pathological changes encountered, some points in the anatomy, gross and histological, and physiol- ogy will be briefly reviewed. The testicles are relatively large, measuring 14 to 17 em. in length, including the epididymis, and 6 to 8 em. in diameter. The tunica albuginea is quite thin and consists of connective tissue which is rich in elastic fibers. On account of its distinct connective tissue structure it is not distinguishable from the tunica vaginalis which is fused with it. Inside the tunica al- bnginea is a loose layer of connective tissue, which, on account of its rich supply of blood vessels, is termed the tunica vaseulosa. 420) Herpert lL. GuuMAan The parenchyma of the testis is of a yellowish gray color. The mediastinum testis is an axial connective tissue structure run- ning through the gland, star shaped on section, and sending numerous connective tissue strands between the lobules. The lobules consist of the seminiferous tubules, which, on account of the courses they take in the different regions, are divided into three groups. The peripheral tubules are the much-contorted tubuli contorti. These join up to make the tubuli recti, which near the mediastinum break up to form a network, the rete testis. The rete proceeds through the mediastinum, to form the efferent ductules which break through the tunica albuginea, to form a part of the head of the epididymis. The tubuli contorti, which form the spermatozoa and make up most of the parenchy- ma, have a width of 120 to 200 microns. The lobules are made up of a peripheral thin membrana propria and the seminal epithelium, which consists of the essential sperm-forming cells and the cells of Sertoli. The sperm cells are many-layered, consisting of the peripheral layer of spermatogonia. These in turn divide, giving rise to a more central layer of primary spermatocytes, which im turn divide through secondary sperma- tocytes, spermatids, and finally the spermatozoa are formed. The cells of Sertoli are large cells of indistinct outline, sending protoplasmic processes out into the lumen. In the final stage of transformation of the spermatids into spermatozoa, the latter bury their heads in these protoplasmic processes of the Sertoli er nurse cells which furnish them with nutritive material. F'nally the spermatozoa mature and become free in the lumen. The interstitial connective tissue between the tubules contains numerous large interstitial cells. They are comparatively deli- eate, shehtly granular cells with abundant protoplasm which contains many fat globules. These cells furnish an internal secretion which governs the development of the secondary sexual characters. The head of the epididymis is made up of lobules formed by the much-coiled efferent ductules proceeding from the rete. These ductules unite to form the body of the epididymis, which remains quite coiled and runs along the posterior medial part of the testicle, to which it is more or less closely attached. At the lower extremity of the testicle it forms the tail of the epi- didymis, finaiil ending in the ductus deferens. The epididymis is lined by a_ pseudo-stratified, columnar, ciliated epithelium. | GENITAL INFECTIONS IN THE BULL 4?1 Outside this is a membrana propria, a circular muscular layer, and a connective tissue coat. The vas deferens is quite narrow (2 mm.) and runs from the tail of the epididymis to the colliculus seminalis, where it empties into the uretha. At first it is lined by epithelium like that of the vas epididymis, but changes over into a stratified columnar type with occasional motionless cilia. The tunica propria is a thin fibrous layer. The submucosa consists of thin connective tissue. Three muscular coats are present, an inner thin longitudinal layer, middle circular, and outer longitudinal layer. These are surrounded by the adventitia, made up of connective tissue, elastic fibers and scattered longitudinal muscu- lar cells of the internal cremaster muscle. The function is the conveyance of the spermatozoa with some of the seminal fluid from the epididymis to the ejaculatory duct. Near the posterior part of the bladder the two ducts are in close apposition, and for 10 to 12 em. dilate to form the ampulle. Here the mucous membrane becomes much plicated and not unlike the folds at the fimbriated end of the oviduct. The seminal vesicles are compact glandular structures lying on either side of the median line, dorsal to the ampulle and ventral to the rectum. In the mature bull they measure 10 to 12 em. in length, 4 em. in width, and about 21% to 3 em. in thick- ness. The glands are quite tortuous and are often asymmetrical in size and shape. The excretory duct of each opens into the urethra in common with the ampulle of the vasa deferentia. Microscopically the gland consists of vesicles lined by pseudo- stratified columnar epithelium. On the outside of the gland is a thick connective tissue layer which sends thin trabecule in between the vesicles or alveoli. The function is to supply the principal fluid content of the semen. Spermatozoa occasionally work up into the lower part of the gland, but ordinarily the fluid is a gray, milky, mucoid material, containing few or no spermatozoa. . The colliculus seminalis is a rounded prominence about 2 to 3 em. in length, at which open the ducts of the vas deferens and seminal vesicles by small slit-like openings. There is no distinct ejaculatory duct in animals. The function of the col- liculus is to prevent admixture of urine with ‘the semen by shutting off the upper part of the urethra during ejaculation. The prostate consists of two parts, which are continuous with HERBERT L. GILMAN Fig. i—Normal testicle of bull. (High power magnification.) Fig. 2--Testicle of bull, showing marked desqua- mation and degeneration of the seminal. epi- thelium lining the tubules. (High power.) SE <9 GENITAL INFECTIONS IN THE BULL Fig. 3—Normal seminal vesicle of bull, (High power.) Fig. 4—Seminal vesicle of bull, showing advanced stages of degeneration and desquamation. The lining membrane of the vesicles has disappeared and the lumina are filled with detritus and exudate. (High power.) 424 Hersert L. Gruman Fig. 5—Normal ductus deferens of bull. (High power.) Fig. 6—Ductus deferens of bull, showing desqua- mation of the lining membrane and exudate into the tube lumen. (High power.) GENITAL INFECTIONS IN THE BULL 425 each other. The body is 3 to 4 cm. in width, 2 cm. long, and 1 to 1.5 em. in thickness, extending across the dorsal surface of the neck of the bladder at the origin of the urethra, and over the colliculus seminalis. The pars disseminata surrounds the pelvic part of the urethra. Dorsally it is about 10 to 12 mm. thick and ventrally thins out to about 2 mm. The gland has a branched tubular structure, the interlobular tissue of which contains much unstriped muscle. The prostatic ducts open into the urethra in rows, two of which are between two folds of the mucous membrane that proceed backward from the colliculus seminalis; two other series occur on either side, lateral to the folds. The secretion, the function of which is to furnish part of the seminal fluid and the principal stimulant to the motility of the spermatozoa, is of an acid reaction, milky, and quite albuminous. The Cowper’s glands (bulbo-urethral) are paired, oval struc- tures, about 3 by 2 em. in size and situated on either side of the pelvic part of the urethra close to the ischial arch. They are covered with the urethral muscle, and both empty into the urethra by a single duct. The structure is compound tubular mucous glands, the gland tubules of which are lined with a single layer of cubueal epithelium, and the ducts with two or three layers of similar cells. The semen when freshly ejaculated is a cloudy, tenacious, more or less coagulable fluid rich in albumen. It is weakly alkaline in reaction, and contains 80 to 90 per cent of water. Of the solid constituents there is 40 per cent of ash, of which three-fourths is calcium phosphate. Besides the spermatozoa, the semen frequently contains epithelial cells, leucocytes, con- centrie ambyloid concretions and lecithin bodies. When cold, characteristic phosphoric acid salts are precipitated. The fluid content is the product of the tubules of the testicles, their ex- cretory ducts and the accessory sexual glands. This is prin- cipally secreted by the ampulla or pars glandularis of the vas deferens and the seminal vesicles. These secretions are thick, homogeneous and gelatinous, resembling sago soup. The amount of semen ejaculated is about 5 to 10 e.e. The spermatozoon of the bull consists of two principal parts, the head and the tail. The head contains the nucleus and is solely for the fecundation of the ovum, while the long and thread- like tail is for motility. The former, which is comparatively 426 Herspert L. GILMAN large, is divided into a darker staining posterior part, an an- terior lighter part, and often a still lighter oval area between the two. On the anterior part of the head is a sharpened edge, the acrosome, which serves the purpose of perforating the ovum. The tail is divided into three parts: connecting piece, principal part, and terminal filament. An axial filament runs through the entire length of the tail. The connecting piece, which is the thickest and strongest part, joins the tail proper to the head. It consists of the central axial filament, a spiral filament around this, and an outer mitochondrial covering. The principal part consists merely of the axial filament and a thin outer covering, while the end piece is quite thin and is made up solely of the uncovered axial filament. The length of the entire spermatozoon is 75 to 80 microns, including the head. The head is 9.5 microns long and 5.5 microns wide. The spermatozoa are nonmotile when discharged from the testicle, and do not become motile until they come in contact with the prostatic secretion (Ellenberger, 13). The technique used in the collection and staining of the sper- matozoa is essentially the same as that described by Williams (9). The methods used in removing the genital tracts and cul- tural methods of the organisms are identical with those described by Carpenter and myself in the study of the female genital tract. PATHOLOGY The pathological changes encountered are quite numerous, but merely the nonspecific types will be considered here. The testicle seldom presents gross alterations of structure ex- cept for abscessation, which, according to Williams, occurs more frequently in the bull than in any other domesticated animal. He also states that arrest in the development of the testes by which they remain soft, flaccid and somewhat smaller than nor- mal is not uncommon. The epididymis is occasionally the seat of necrotic processes,.as evidenced by swelling, tenderness and abscess formation. In two instances cases were encountered in which the head of the epididymis was much enlarged and firm, due to a chronic proliferative inflammation. On section the ef- ferent ducts of the epididymis were much atrophied, the lining membrane was degenerated and desquamated, while the inter- stitial tissue was much increased in amount. Abscess forma- tion may oceur either here or at the tail of the epididymis, Z GENITAL INFECTIONS IN THE BULL 427 where it is more frequently encountered. Occasionally the ducts show slight desquamation and some degeneration of the lining epithelial cells, without any gross manifestations. Microscopi- cally, the testicular tissue is the seat of pathological processes. Perhaps the most frequent condition encountered is desquama- tion and degeneration of the seminal epithelium. The process may be seen in various stages, from only slight exfoliation to almost complete disappearance of the essential semen-forming cells:(figs. 3 and 4). In one instance total disappearance of the seminal tissue with marked thickening of the membrana propria was encountered, associated with an extensive increase of inter- stitial tissue. In every genital tract examined (146) I have failed to find one in which there were not present many small strands of connective tissue between the tail of the epididymis and the adjacent part of the parietal layer of the tunica vagi- nalis. In some instances infection was present, while at other times the strands were evidently the result of some previous in- fection. The ductus deferens frequently shows degeneration and ex- foliation of the lining membrane (figs. 5 and 6), and when this occurs it is usually associated with changes in either the testicle or seminal vesicles. In man the duct becomes occluded by a chronic inflammation, but so far I have failed to find evidence of this condition in the bull. Undoubtedly, however, this condition does occur ocea- sionally. The seminal vesicles are perhaps the most common seat of infection and pathological changes. The acute form of inflam- mation is accompanied in some instances by enlargement either of one or both glands. On rectal palpation this may be quite easily distinguished, and pinching of the part causes a distinct flinching and evidence of pain. Together with the enlargement it becomes somewhat softer and flabby or even hard in the early‘stages. On microscopical examination there is usually extreme hyperemia of the entire part, with varying degrees of desquamation and degeneration of the epithelium lining the vesicles. The normal clear mucous secretion becomes mixed with fibrin, leucoytes and cellular débris. In the more severe types the entire epithelium becomes desquamated and neerotie with more or less loss of definite structure in the gland. Cystie inflammation may oceur, in which 428 HERBERT L. GILMAN ease the cysts may involve only one or two lobules or occasionally the entire gland. Abscess formation is not infrequent, in which case the entire gland becomes made up of small abscesses, or the entire gland may be one large necrotic encapsulated mass. Chronic inflammation is evidenced by a distinct enlargement and firmness of the gland. On microscopical examination most of the gland is composed of firm sclerotic tissue in which here and there one finds remains of the degenerating vesicles. This type may or may not be accompanied by adhesions to neighbdring parts. In one ease encountered, the glands were much enlarged, sclerotic and almost lost in a large mass of adhesions. The prostate and Cowper’s glands no doubt are frequently involved in inflammatory processes. However, unfortunately, these glands were more or less neglected in the work, with the result that changes may have been present though not detected. The semen presents a most interesting and essential subject for routine examination and study. While the advances made so far have been more or less elementary, the work has demon- strated that its study is a very valuable aid in the diagnosis of infertility in the bull. Not only is the seminal fluid fre- quently the carrier of several varieties of bacteria associated with genital infections, but in a large percentage of cases the essential spermatozoa are abnormal either in their motility or morphology or both. Likewise the seminal fluid is subject to changes which are often detrimental or injurious to the existence of viable spermatozoa. ‘ : It has been demonstrated that very frequently the bull harbors streptococci, micrococci and other organisms associated with genital infections in the female. These organisms become localized in the seminal vesicles and testicles, and during ejacu- lation of the semen are discharged and deposited in the vagina of the female. Bacteriological studies on the semen are not on the whole entirely satisfactory, but are sufficient, I believe, +o demonstrate this fact. The method employed consisted in dench- ing and disinfecting the vagina and sheath after the method suggested by Williams. Samples of vaginal mucus were then taken for study, following which the semen was removed after coitus. The vagina usually harbors organisms, though on smear but a few are ordinarily seen. In the bulls of high fertility the semen obtained by this method failed to show many organisms, most or all of which probably came from the vaginal mueus. GENITAL INFECTIONS IN THE BuLuL 429 The semen from many sterile bulls or those of low fertility, however, clearly showed that a large number of organisms must have been eliminated with the semen. Likewise the organisms isolated agreed culturally and morphologically with those found in diseased seminal vesicles and testicles. Perhaps some of these organisms were brought in by a penis contaminated by the ex- terior of the cow, but the number and type of organisms found would tend to make this source of contamination improbable. The seminal fluid, besides being frequently laden with bacteria, is In many cases abnormal. In one instance the fluid was quite thick, of a yellowish green color, and of a distinct acid reaction. The secretion from the seminal vesicles was later found to be of this same character, and the glands had undergone diffuse ab- scess formation. The fluid may frequently contain pus, or rarely blood. Decrease in the amount of fluid, or a thin watery condition, is not infrequently encountered. In one instance the semen appeared in all ways like a thin, serous fluid. Distinct changes in the motility and morphology are, however, the main features to be looked for.in the study of the semen. Not infrequently complete azoospermia is met with, however, with quite distinct changes in the character of the seminal fluid. In the examination of the motility the semen is best studied with the use of a warm stage, or if this is not available, a drop of the semen may be placed Upon a warm slide. Motility may be observed with the aid of the low or high power lens, though often it is best to use a cover-glass and the oil immersion. Nor- mal vigorous spermatozoa show a vigorous lasting motility which persists for hours, or even days under proper conditions. Ab- normalities of motion are manifested by sluggish motion of all or part of the cells present, abnormal types of motion, or, in many instances, entire absence of motion. Anatomical changes encountered were mostly confined to loss of the tail, and irregularities in staining of the nucleus. Some specimens show mostly headless spernis in the field, while others show but a few without tails. The irregularities in staining are manifested by unusual difficulty in obtaining a clear dif- ferential staining of the head. Abnormal forms such as sper- matozoa with two heads, thickening of the connecting piece of the tail, diminutive size of the head, ete., are by no means un- common. Their significance is not understood, but they are 430 Herpert L. GILMAN probably the product of some derangement in the seminai epithelium. The bacteriology of the male genital tract was studied upon the genitalia of 10 normal young (6 to 8 weeks) calves, 2 mature fertile bulls and 12 mature infertile bulls. Four aborted fetuses and 5 calves dying from calf infections (scours and pneumonia) were also cultured, as well as 11 bulls slaughtered at an abat- toir. In the latter group no history was available, as was the case in nearly all of the others. The seminal vesicles and testicles of the normal calves were sterile in all but two cases. From these two tracts a staphylo- coccus was isolated from one seminal vesicle of each. In one aborted fetus a streptococcus was isolated from both the seminal vesicles and testicles, this same organism being obtained from the heart’s blood. In another Bacillus colt was isolated from all parts, with a general colon septicemia. Micrococci and strep- tococci were in several instances obtained from the seminal vesicles, testicles, or both, from this group of animals. In each instance the organisms were identical with those isolated from other parts of the fetus or calf. Adult bulls of known fertility were naturally difficult to ob- tain, only two being available for examination. These were from the experimental herd kept by the department, and have always had an excellent breeding history. One bull had a severe attack of scours when a few weeks old, while the calfhood history of the other is not known, he having been bought after sexual ma- turity. Cultures from the genital tract from the first-mentioned bull were entirely negative except for the presence of a strepto- coccus isolated from the left epididymis and left scrotal sac. All organs were normal except for numerous fine connective strands running from the tail of the epididymis to the adjacent part of the parietal layer of the tunica vaginalis in both testes. The tract of the other failed to show any organisms. The only evidence of any abnormality was the presence of the same fine connective tissue strands as were in the other tract. In the mature sterile bulls, or those of low fertility, there was a comparatively wide variation in the type of organisms en- countered, though the streptococci and micrococci were the most common invaders. The tail of the epididymis and the seminal vesicles are the most common seat of infection, the latter being infected in nearly all sterile bulls so far examined. One very “ ee GENITAL INFECTIONS IN THE BULL 431 interesting case noted was that of a bull with a history of sud- denly becoming sterile. The semen was semifluid, greenish yel- low in color, and contained very few nonmotile spermatozoa. On postmortem the vesicles had undergone abscess formation and contained the same greenish yellow material which had been dis- charged during copulation. Streptococcus hemolyticus and Pseu- domonas pyocyaneus were isolated from both vesicles. In one bull a watery condition of the semen was found to be due to eystie degeneration of the vesicles. The tracts obtained from abattoir bulls were not always in- fected, but most of those examined bacteriologically demon- strated that the infection is quite widespread. Gross and histo- logical changes of the seminal vesicles were by no means un- common, as well as degeneration and desquamation of the semi- nal epithelium of the testes. Micrococcus aureus and albus were isolated in nearly all cases, though usually associated with Streptococcus viridans or hemo- lyticus. When checked up with the organisms found in the semen, the two appeared to be identical in every way. Bacillus colt was encountered but twice, and then in the vesicles. I have so far failed to obtain Bacillus abortus from any tract, either by direct culture or guinea-pig inoculation. The agglu- tination tests with B. abortus antigen were all negative except for two bulls from the abattoir. In three instances an organism was isolated from the seminal vesicles which agreed culturally and morphologically with B. abortus, but guinea-pig inoculation with the cultures of the organism failed to identify it as the Bang bacillus. Likewise an antigen made from the cultures were not agglutinated by positive abortion serum. Clinically, the diagnosis of sterility rests principally upon an examination of the semen, testicles and seminal vesicles. As stated before, in examining the semen one should note the re- action, quantity of sediment and character of the fluid. The spermatozoa should be examined for the relative number present and their motility, which may be vigorous, sluggish or absent. In staining one must look for poor staining of the head and for morphological abnormalities. The presence of bacteria, leuco- eytes or an unusual number of desquamated cells is to be noted. The testicles in each case should be examined for abscess forma- tion, atrophy or any chronic enlargement, especially of the epididymis. 432 Herspert L. GmMAN The seminal vesicles in many cases will, on physical examina- tion, be enlarged and firm, soft and flabby, or otherwise diseased. Abscess formation, or cystic degeneration is not uncommon, and occasionally the glands ean not be palpitated, due to their being lost in a dense mass of adhesions to neighboring structures. Sen- sitiveness on pressure is usually evidenced by a distinct flinching on the part of the animal. On the other hand, the vesicles may show no macroscopic evidence of inflammation, it being upon on microscopical section that the gland is found diseased. The spermatozoa will not live in a fluid not suited to their delicate requirements, and since the vesicles furnish most of the seminal fluid which is generally materially altered when the gland be- comes infected, these glands should be given a careful examina- tion. Abnormal semen may not be associated with diseased testes, but this is almost invariably the case with diseased seminal vesicles. While the work so far is but in its infaney, the results are such as to demonstrate that the bull often becomes infected and may be a dangerous source of infection in the herd. The semen is very frequently abnormal and contains bacteria which are similar to or identical with those isolated on postmortem examina- tion from diseased genitalia, especially the seminal vesicles. Likewise these organisms found in the semen are very frequently associated with a wide variety of genital infections in the female. Clinically, as stated by Williams (12) : ‘‘The cows served by one bull conceive in larger percentage than those served by another. Cows pregnant by a bull of low fertility abort in larger ratio than cows bred to bulls of high fertility. Those cows which are sterile after having been bred to bulls of low fertility often prove persistently sterile when bred to highly fertile bulls. In these cases not only has the bull ejaculated an infection fatal to the spermatozoa and ovum of the coitus concerned, but he has im- planted in the genital tract of the cow an infection which pre- vents fertilization in the future by a fertile bull.”’ One valuable purebred animal coming to my attention had previously given birth to four normal healthy calves. She was then bred to a pedigreed bull whose history was not looked into. Abortion took place at eight months, with death of the calf, and the cow barely recovered from a severe septic metritis. It would not be surprising to find that her breeding career was at an end. GENITAL INFECTIONS IN THE BULL 433 In one purebred herd attended by Dr. W. W. Williams, serv- ice to certain bulls was invariably followed by cervicitis, which later developed into salpingitis, leaving cow after cow hope- lessly sterile. The semen and, as found later, the seminal vesicles of these bulls contained virulent streptococci and the seminal vesicles were badly diseased, as demonstrated by clinical and histological examination. . The finding of the fine connective strands between the tail of the epididymis and the adjacent part of the parietal layer of the tunica vaginalis in every bull examined so far demonstrates the extent of the presence of some infection of the male genital tract at some time during life. The presence of organisms in the geni- tal tracts of aborted fetuses and of calves dying from some form of ealf infection may signify that infection localizes in the parts early in life, either to die out or lie dormant until sexual matur- ity, when it becomes intensified, especially by excessive sexual strain. The fact that the tracts from the healthy calves failed to show any bacteria, while those from diseased calves and aborted fetuses usually harbored bacteria, tends to the belief that bac- teria are not normally found in the male genital tract. This appears to be so in the young, at any rate. Of the two adult bulls with a good breeding history, one showed no organisms, and the other infection of only one epididymis and scrotal sae. It is in- teresting to note that the bull harboring the infection, which. by the way, was not discharged in the semen as far as I could find, had had the attack of scours while a calf. Both bulls, however, were used moderately and were douched before and after each service. The presence of the fibrous strands on the epididvmis shows that infection had been present in both bulls. Hema- togenous origin of the infection is always possible even in the adult. On the other hand, while there is no convincing evidence to support it, urethral infection from serving diseased cows is very probable. ; BIBLIOGRAPHY 1. CARPFNTFR, C. M.. Professor of Bacteriology, New York State Veterinary College, Cornell University. 2. BANG, RB. Die Atiolomie des seuchafen (infectidsen) Verwerfens. Zeit. Thiermed., Band I, S. 241, 1897. Das Senchenhaften Verwerfen der Rinder. Arch. Wiss. u. Prakt. Thierheilk., Band 33, S. 312, 1907. 3. Report of English Commission on Epizootic Abortion, Appendix to Part I, p. 17, 1909. 4, HapLEy, F. B., and LotHe, H. The Bull as a Disseminator of 434 Hersert L. GIuMAN Contagious Abortion. Jour. Amer. Vet. Med. Assoc., vol. 50, 1916-17, p. 1438. 5. Buck, J. M., CREECH, G. T., and LApson, H. H. Bacterium Abor- tus Infection of Bulls (Preliminary Report). Jour. Agr. Research, August, 1919. 6. SCHROEDER, E. C., and Cotton, W. E. The Bull as a Factor in Abortion Disease. 7. MCFADYEAN, SHEATHER and MINETT. Researches Regarding Epi- zootic Abortion. Jour. Compar. Path. and Ther., vol. 26, p. 142, 1918. 8. HADLEY, F. B. Contagious Abortion Questions Answered. Wis. Agr. Exp. Sta. Bul. 296, Sept., 1918. 9. WILLIAMS, W. W. Technique of Collecting Semen for Laboratory Examination with a Review of Several Diseased Bulls. Cor- nell Veterinarian, vol. 10, No. 2. 10. WiLLIAMS, W. L. The Diseases of Bulls. Cornell Veterinarian, vol. 10, No. 2. 11. WILLIAMS, W. W. Diseases of the Bull Interfering With Repro- duction. Jour. Amer. Vet. Med. Assoc., vol. 2 (n. s.), No. 1. 12. WILLIAMS, W. L. Report of New York State Veterinary College, Cornell University, 1919-20. 13. ELLENBERGER. Vergl. Mikroskop. Anatomie der Haustiere, vol. 2. VETERINARY PRACTICE IN ST. KITTS Dr. Ernest F. Jardine, Government Veterinary Surgeon for St. Kitts, British West Indies, in sending in his application for membership in the A. V. M. A., sends the following brief but interesting description of his environment and work: St. Kitts is an area of about sixty-eight square miles, has a population of 25,000, chiefly black. It is a mountainous island, the tallest mountains being from 3,000 to 4,500 feet high. There is only one road, practically speaking, and that one leads around the island, so if you start at a given point and keep going you will reach the place from which you started. The island is very healthy, and, many of us think, very pretty, having the high mountains with green verdure in the centre, and the sea always in view. I have a contract with most of the estates on the island to do the veterinary work at so much per quarter. They pay ac- cording to the number of animals kept, irrespective of the dis- tanee. An estate one mile from town may pay three times as much as one fifteen miles away. That being the case, I leave colic draughts for emergency cases. In the contract everything is included from ecastrating madam’s tom eat to any major operation on the most valuable animal on the place. The amount of work I have to do would be worth twice as much in a big coun- try, but living and house rent and one or two other items are much cheaper; the one counterbalances the other. cll PO = . pc ee Yes SOME STUDIES IN SWINE ABORTION * By Frep Hayes Umversity of California, Davis, Calif. THE MATERIAL presented in this paper is the result of about a year’s investigational work on swine abortion. In May, 1920, Hayes and Traum reported the results of some preliminary investigations made in three outbreaks of swine abortion in Cali- fornia caused by Bacterium abortus (Bang). No eultural, morphologic or biologie difference could be noted between the isolated swine strains and those from cattle, except that the porcine strains grew more abundantly and did not seem to de- velop such a deep brown pigment as did the bovine strains. This difference, however, was not constant. Since writing the above-mentioned report, absorption tests with rabbit sera im- munized against three hog strains from our three different out- breaks, rabbit sera immunized against two bovine strains, guinea- pig serum immunized against one bovine strain, and serum from a naturally infected cow have shown no difference between the organism of bovine origin and those in swine. Porcine strains proved, on the whole, to be more virulent for guinea-pigs, caus- ing, with a few exceptions, involvement of the testes and one or both radiocarpal regions, also general adenitis. Thirteen of 22 guinea-pigs moculated with porcine strains succumbed within two months, while none of the 12 inoculated with bovine strain died of infection during that period. The dose for each guinea- pig in the above series was similar. Before publishing our findings a search was made through the index pages of publications in which the etiology of abortion in swine would be discussed. ‘This revealed to us reference to but one report in which Bacterium abortus (Bang) had been in- eriminated as the cause of a natural outbreak of this disease. This reference called attention to the work of Good and Smith, wherein they described an extensive outbreak of swine abortion in Kentucky caused by Bacterium abortus (Bang). Traum in 1914 had isolated Bacterium abortus (Bang) from the liver, stomach contents and kidney of an aborted fetus from a herd of swine in which many abortions had oeeurred. No exact history ‘Presented at the fifty-eighth annual meeting of the American Veterinary Medical Association, Denver, Colo., September 5-9, 1921. 435 436 Frep HaAyEs was obtained or further work done in this herd. Later (1919) Connaway, Durant and Newman of Missouri produced abortion in pregnant sows by artificial infection with Bacterium abortus (Bang) of bovine origin. Doyle and Spray reported in August, 1920, the isolation of an organism indistinguishable from Bac- terium abortus (Bang) from a number of outbreaks of swine abortion in Indiana. The report of the Committee on Diseases of the United States Live Stock Sanitary Association for 1920 makes reference to the work of Schlegel in Germany wherein Bacterium abortus (Bang) was found to be the cause of certain outbreaks of swine abortion. Other investigations with this or- ganism in relation to abortion in swine are known to be in progress at the present time. These reports, combined with the clinical observation of abortion in swine for many years, indicate increasing evidence that the abortion bacterium is of greater significance in this species than has been generally recognized. The fact that hogs have for many years consumed uncooked dairy products and associated with infected cattle giving off virulent abortion organisms, without serious epidemics of swine abortion developing until recently, is not to be considered lightly in the future if the maximum fertility of breeding stock is to be maintained. Our principal investigations have been carried on in one herd of swine naturally infected with Bacterium abortus (Bang) and with a small herd artificially infected. The results of the ex- periments herein reported and the interpretations placed upon them can only be suggestive. However, they may be of value as data are accumulated. VIRULENCE OF ISOLATED PORCINE STRAINS In the three infected herds reported in a preliminary paper Bacterium abortus (Bang) was so abundant in the fetuses, fetal fluids and fetal membranes, and so easily cultivated, that little doubt was entertained as to its etiological importance. To test the power of the strains obtained from the three outbreaks to produce abortion, six first-time pregnant Duroe sows, negative to the agglutination test, were given emulsions of 48-hour-old cultures of our porcine strains. Three of these sows each re- ceived 1.5 mils of a heavy emulsion intravenously. The other three each received 10 mils of one of the organisms in their crit el ~~ ew el he Za : Lf es a fe a ? Some Srupres iN Swine ABortTION 437 mash twice a day for two consecutive days. Weekly agglutina- tion tests were performed until the subsequent farrowing. Sow No. 1119 was fed culture 1 (7) in the manner stated above. Complete agglutination of bovine and swine antigens with 0.02 ¢.c. serum oceurred on the thirteenth day after feed- ing. The agglutinating titer of her serum never went appre- ciably higher than this, and she farrowed ten live but weak looking pigs 83 days after being fed. Bacterium abortus (Bang) was isolated from the fetal membranes and from the stomach contents of one of the pige which was killed immediately after birth. Sow No. 1124, fed in a similar manner with culture 3 (2), ag- glutinated bovine and swine antigens in 0.01 ¢.. of serum on the twenty-first day after feeding, and the titer remained at this point for 53 days longer. Her serum then became negative in 0.02 ¢.¢., and she farrowed nine healthy looking pigs 89 days after feeding the culture. Ageglutination remained negative in 0.02 ¢.c. for about ten months more, at which time she again farrowed nine live pigs. Bacteriwm abortus (Bang) was isolated from the fetal membranes at first farrowing but not at the second. Sow No. 1127, fed in a similar manner with culture 1 (1), ag- glutinated bovine and swine antigens with 0.01 ¢.c. of serum on the twenty-first day after feeding, and the titer remained at this point for 28 days longer. Agglutination then became complete with 0.002 ¢. c. for 21 days more, at which time she farrowed ten normal appearing pigs. On the day she farrowed her blood serum agglutinated in 0.005 ¢.¢., but returned to positive in 0.002 ¢.c. in a week and remained at this point for eight months longer. This sow later farrowed eight live pigs and one dead one. Bacteriwm abortus (Bang) was not isolated from the fetal tissues at either farrowing. Sow No. 1118 received 1.5 ¢.c. of a heavy emulsion of 48- hour porcine strain culture 1 (7) intravenously. On the eighth day after injection her blood serum gave complete agglutination with 0.002 ¢.c. On the twenty-seventh day after injection she aborted fetuses in about the sixth week of pregnaney. Bacterium abortus (Bang) was readily isolated from the fetal membranes and stomach contents of a fetus. For about two months after the abortion her blood continued to give complete agglutination with 0.002 ec. During the next nine months she was positive in 0.02 ¢.c. but not in smaller quantities. This sow farrowed 438 Frep HaAvyEs ten live but weak pigs and one dead pig practically seven months after the abortion. Bacteriwm abortus (Bang) was obtained from the stomach contents of the dead pig. Six months later she again farrowed seven live weak and four dead pigs. Sow No. 1126 received an intravenous injection of porcine culture 1 (1). On the eighth day her blood serum gave complete agglutination with 0.002 ¢.c. The agglutinating titer remained at least this high for thirteen months against both bovine and swine antigens. Twenty-five days after the injection she far- rowed eight live normal pigs. Bacteriwm abortus (Bang) was obtained from the fetal membranes and the stomach contents of one of this litter killed before suckling. Seven months after the injection she again farrowed five normal live pigs and six months later she gave birth to nine live normal progeny. Sow No. 1121, supposed to have been pregnant when pur- chased for the infection experiment, proved not to be so, but received an intravenous injection of culture 3 (2). She was bred three months after the injection. Her blood serum ag- glutinated bovine and porcine strains with 0.002 ¢.c. on the eighth day. This power was maintained for a little more than six months, and at the seventh month after injection she far- rowed nine live pigs and one dead pig. Three weeks later pos- terior paresis and a vaginal discharge developed, from which she improved slowly during the next two months. When able to walk alone, though with difficulty, she was bred. A month later she was butchered and found to be pregnant. There was no visual evidence of infection in the pregnant womb. Bacterium abortus (Bang) was not isolated. A review of this experiment in infection with Bacteriwm abortus (Bang) indicates that actual abortion is produced with difficulty by artificial infection. Sow No. 1118, injected intra- venously, gave a rather classical result by aborting in 27 days. The effect of feeding and injection upon the other five sows, however, was benign, notwithstanding evident implantation of the infection. Their breeding qualities were not materially lessened, although a few of the pigs were born weak and did not long survive. Some strains of Bacteriwm abortus (Bang) are known to lose their virulence under laboratory cultivation while maintaining their biological ability to produce agglutinins. The cultures used in these experiments were isolated from virulent natural Some StrupiEs IN SWINE ABORTION 439 outbreaks and had not been under laboratory cultivation for more than a month. In five of the six sows infection became es- tablished as indicated by the production of agglutinins and by the recovery of Bacterium abortus (Bang) from the fetal tissues. In these artificial infection experiments just described, and also in an experiment with eight young boars and barrows fed or injected with mixed strains of porcine abortion cultures, anti- bodies appeared in 0.002 ¢.c. of serum in about eight days in those injected, and in from 21 to 30 days in those fed with cultures. There apparently are some contributing factors in field infee- tion that do not oceur in artificial attempts to produce abortion with Bacterium abortus (Bang). AGGLUTINATION REACTIONS IN NATURALLY INFECTED HERDS In March, 1920, monthly agglutination tests were begun in a naturally infected herd (Herd 2) of 60 purebred swine in which six abortions had occurred between February 8 and March 2, 1920. Quantities of 0.02, 0.01, 0.005 and 0.002 ¢.c. of the serum to be tested were added to 1 ¢.c. of the antigen. Both porcine and bovine antigens were used in every test. This was the regu- lar routine, although higher dilutions were frequently made. Whenever complete agglutination occurred with 0.01 ¢.c. of serum the hog was designated as a positive. On the first test there were 29 positive, including the 6 abort- ers, and 31 negative. One month later 6 had changed from nega- tive to positive and 3 from positive to negative, although these new negative sows did not continue to be negative throughout the year. A study of these agglutination test records for the year shows that practically all of the reactors continued to agglutinate in amounts of serum from 0.02 to 0.005 ¢.c. for the entire time, and that only 11 animals out of the 48 tested monthly throughout one year remained: consistently negative. It is difficult to analyze the agglutination records for interpretation and to account for fluctuations that were obtained in the titer of serum from some of the hogs at different times. The majority of the monthly re- sults, however, were very consistent throughout the year. At the end of the year there appears to be the beginning of a fall in the agelutinating titer in the animals that were regularly positive during this period. Pregnancy and the farrowing act do not 440 Frep Hayes seem to alter consistently the quantity of agglutinins in the blood serum. AGGLUTINATION TESTS AND INFECTION IN Pigs It has been of interest to observe whether agglutinins are transmitted from positive mothers to their offspring at birth, and also to note whether agglutination will oceur in pigs nursing infected mothers. We have not found agglutinins present in new-born pigs, but only a small number have been tested. Mar, 7 1921 do. 005 . Mar. 14, 1921 do. = } April 5, 1921 712 0061 Died 22 hours. 5 2,240 do. } do. 712 ©0001 do. 6 | 3,000 Used Mar. 14, 1921 VD ea ok: April 5.1921 | do. 00] Lived. tf | 2,420 Normal do. do. -001 do 8 | 3,040 Used Feb. 25, 1921 MD 0001 do. 9 | 2,100 Normal | do. do. 0001 |- do. | Mar. 2, 1921 do. 001 | Died, 48 bours. 10 | 2,240 | do do. do. 001 Died, 19 hours. 1l , 1,840 Used | April 5, 1921 do. 001 Died, 22 hours. 12 2,200 Normal | do. do. 001 =| do. 13 3,700 Used | Mar. 23, 1921 MR 001 Died, 6 days. 14 | 3,000 Normal | do. ita do: .001 | Died, 24 hours. 15 2,360 Used | April 5,1921 | do. 001 Died, 22 hours. 16 ; 2,900 | Normal do. do. 001 Died, 23 hours. 1 Rabbits designated as “‘used’’ had received one or more previous injections of hemor- rhagic septicemia organisms and were used in these experiments for the purpose of noting any degree of immunity conferred by these injections. Suck rabbits were generally used in pairs, one norma] and one “‘used,’’ and in only one instance (rabbit No. 13) did the “‘used”’ rabbit show any appreciable resistance above that shown by the normal rabbit. DISCUSSION OF THE VIRULENCE TESTS Rabbits (Nos. 3-7) injected intravenously with varying amounts (0.1 to 0.0001 ¢.¢.) of 24-hour bouillon cultures of the strain isolated directly from the lung, in single or repeated doses, failed to succumb. The doses injected were many times the dose of a virulent culture necessary to kill rabbits. Four injections, at about weekly intervals, failed to immunize a rab- bit (No. 4) against a virulent strain of Bacterium bovisepticum (No. 712), the rabbit dying as soon as the normal control (No. 5). HEMORRHAGIC SEPTICEMIA ORGANISM 457 In the case of the strain isolated directly from the muscle (MD) all rabbits receiving a dose of 0.001 ¢.c. of a 24-hour bouillon culture died promptly. Two rabbits resisted a dose of 0.0001 c.c. One of these (No. 8) had been used in a previous experiment, and may have been partially immune. The injection of the same amount in a normal rabbit (No. 9) failed to kill, and when this rabbit was given a second injection five days later, it did not die until 48 hours later, the control (No. 10) dying in 19 hours. This might be interpreted as due to a slight immunity resulting from the first injection, or to some natural immunity. The strain isolated from the muscle through rabbit passage (MR) killed consistently in doses of 0.001 ¢.e. One used rab- bit (No. 13) showed sufficient resistance to withstand a killing dose for over 5 days, sickening and dying on the sixth day. The minimum fatal dose of the culture was not determined. From these few experiments we came to the conclusion that we had been successful in isolating both a virulent and a non- virulent hemorrhagic septicemia organism from the same ani- mal, the virulent type from the muscle and the nonvirulent type from the lung. So far as determined these were typical strains of Bacterium bovisepticum, and identical so far as cul- tural and morphological characteristics were concerned, with the exception of the growth in bouillon as previously noted. Nonvirulent organisms indistinguishable from members of the hemorrhagic septicemia group have been isolated from the re- spiratory tract of various animals (calves, hogs, rabbits) by the present writer on previous occasions. Others have reported similar findings. Likewise, the present writer has isolated viru- lent hemorrhagic septicemia organisms from the lung, heart muscle or blood of animals dying of clinical hemorrhagic sep- ticemia (calves, sheep, hogs, rabbits). This instance, however, is the first where both virulent and nonvirulent organisms have been recovered from the same animal. It is quite likely, how- ever, that nonvirulent organisms have failed to receive recogni- tion when virulent varieties were present to overshadow them. PREVIOUS OBSERVATIONS In a paper (2) read before the Missouri Valley Veterinary Association two years ago, I made the following statement: “Tt would appear from an examination of a large number of strains of these (hemorrhagic septicemia) organisms that there 458 H. Preston HOoskKINS are minor cultural differences between these strains, although certain morphologic and physiologic properties seem to be firmly fixed. There are also slight differences serologically. These sug- gest the possibility of there being a number of types of the organism, just as we have the different types of the pneumo- coccus or the dysentery bacillus. Whether it will be possible to correlate these differences with any important pathogenic prop- erties will be revealed by future investigations. ”’ Manninger (3) has observed the coexistence of virulent and nonvirulent fowl-cholera organisms (Bacterium avisepticum) in 6-months-old bouillon cultures, his attention being drawn to them when transfers were made to agar and two types of colonies developing on this medium. When the two types were separately replanted in bouillon, they showed a marked dif- ference, especially in the character of the sediment. The two types cross-agelutinated. In a recent publication De Kruif (4) has reported some ob. servations on the coexistence of individuals of different degrees of virulence in cultures of the bacillus of rabbit septicemia, isolated from spontaneous infections occurring among the nor- mal animal stock in the laboratories of the Rockefeller Institute. Cultures were made from the heart-blood in 10 per cent defibri- nated rabbit-blood broth, incubated for 24 hours, and then plated out in 5 per cent rabbit-serum agar. Colonies were fished to rabbit-serum broth, incubated and examined, thén streaked on 10 per cent rabbit-serum agar, these cultures being kept for study. ‘ In a series of well-executed experiments De Kruif has been able to isolate two types of organisms from these cultures of the rabbit septicemia organism. These types have several points of difference culturally (bouillon growth and agar colonies), and there is a wide difference between the degrees of virulence for rabbits. These differences may be summarized as follows: TABLE 3.—DIFFERENCES BETWEEN TYPES DESCRIBED BY DE KRUIF | Type D Type G | | Diffuse Granular (flocculates readily) Opaque, fluorescing colonies Translucent, bluish nonfluorescing High Low Bouillon (plain or serum) Agar (serum) Virulence for rabbits | The two types of organisms are morphologically indistinguish- able and possess identical fermentation reactions. Rabbits sur- HEMORRHAGIC SEPTICEMIA ORGANISM 459 viving inoculation with Type G are later found to be able to withstand multiple lethal doses of Type D. Agglutination and absorption tests strongly suggest the antigenic identity of the two organisms. Careful reading of De Kruif’s report leaves somewhat in doubt the exact origin of the two types. The original heart- blood cultures were plated out and the cultures from which he subsequently separated the two types were descendents of single colonies. Yet, after another plating, he was able to get the two types, and these, once separated, appeared to retain perfectly their characteristics of cultural growth and virulence. Hav- ing read the publication of De Kruif, it is perfectly natural to attempt some explanation of his findings, or to reconcile our observations with those of De Kruif. As previously stated, various animals, especially rabbits, fre- quently harbor hemorrhagic septicemia organisms in some part of the respiratory tract. The heart-blood of rabbits in ap- parent health, bled for culture medium purposes, has been found to contain hemorrhagic septicemia organisms. Whether these have been caused to enter the blood-stream, at death, in a mechanical way, incident to the bleeding process, or whether the blood stream harbors a few organisms from time to time, ean not be stated definitely. Animals harboring hemorrhagic septicemia organisms are not necessarily immune to artificial infection with virulent strains of the same organism, as shown by Davis (5). This is not al- ways true, however, of rabbits having subeutaneous abscesses caused by local infections with the rabbit septicemia bacillus. Many of these animals exhibit considerable immunity to arti- ficial infection. With these facts in mind, and with the knowledge that the blood stream may harbor simultaneously two organisms, not necessarily related, it seems easily possible that in a terminal infection, as in rabbit septicemia, we might have two organisms, in this ease closely related, in the blood stream at the same time. In the case of the calf dead of hemorrhagic septicemia, re- ported in this article, we have shown that the animal had in its system, simultaneously, at death, both a virulent and a non- virulent hemorrhagic septicemia organism. The blood was not cultured, but it is quite likely that it contained the virulent strain, and we can not say that it did not contain the nonviru- 460 H. Preston Hoskins lent strain. Neither can we say that the muscle, from which the virulent strain was isolated, did not contain the nonvirulent strain. By the time that it was apparent that we had the two strains, the original material had been discarded. These findings tend to complicate, for the time being, at least, our views on the etiological significance of the hemorrhagic septicemia organisms and their bearing on immunological reac- tions. May the presence of hemorrhagic septicemia organisms of a low virulence stimulate the production of antibodies suf- ficient to protect the host against infection with a virulent epi- zootie strain, or may it have jnst the opposite effect, namely, that of sensitizing the animal and rendering it more susceptible? Can a nonvirulent strain, such as was isolated from the lung of the calf, undergo mutation and become virulent? The writer has been able to change, at will, the character of the growth, in bouillon, of a virulent culture of Bacterium bovisepticum, by simply altering the composition of the medium. Future ex- periments may show that we can alter the virulence in the same way. We have reason to believe that alterations in virulence do take place under natural conditions. In fact, our present views on the nature of the disease are based on that assumption. Some strains of the hemorrhagic septicemia organism retain indefi- nitely their virulence for laboratory animals. Others become avirulent in a comparatively short length of time. To restore virulent properties to a strain that has become avirulent is a difficult task, with our present knowledge. The demonstration that organisms of low virulence suddenly become exalted in the animal body is even more difficult. REFERENCES 1. Kiix, H. C. A simple method of isolating bacteria from patho- logical material. Jour. Lab. and Clin. Med., vol. 6 (1920), No. 2, p. 104. 2. Hoskins, H. P. Some considerations of the hemorrhagic septi- cemia group. Proc. Mo. Valley Vet. Assoc., 1919. (In © preparation.) MANNINGER, R. Ueber eine Mutation des Geflugel-cholera-bazillus. Centbl. Bakt., etc., I, vol. 83 (1919), p. 520. (Abst. in Bul. Inst. Pasteur, vol. 18 (1920), No. 10, p. 330). 4. De Kruir, P. H. Dissociation of microbic species. I. Coexistence of individuals of different degrees of virulence in cultures of the bacillus of rabbit septicemia. Jour. Expt. Med., vol. 33 (1921), No. 6, pp. 773-789. 5. Davis, D. J. Further observations on subcutaneous abscesses in rabbits. The carrier state and its relation to rabbit septi- ee Jour. Infect. Diseases, vol. 21 (1917), No. 3, pp. iv) wal CLINICAL AND CASE REPORTS (Practitioners and others are invited to contribute to this depart- ment reports of unusual and interesting cases which may be helpful to others in the profession. ) PATHOGENIC EFFECTS OF CAPILLARIA WORMS ON CHICKENS By E. L. Strusss and Howarp CRAWLEY Philadelphia, Pa. ON September 6, 1921, three White Wyandotte chickens, three months old, were brought to the laboratory of the Bureau of Animal Industry, Pennsylvania Department of Agriculture, for examination. The owner is a breeder of high-class birds and has a flock of 175, at that time consisting of 50 adult hens, 50 pullets and 75 young chickens. He was having no trouble with the hens or pullets, the infection being confined to the younger members of the flock. At the time given, three or four had died, and nine others were affected. The symptoms were droopiness, slight diarrhea and progres- Sive emaciation, this condition terminating in death after about a week. The flock was being fed plenty of greens, boiled oats, all the mash they wanted. and grain at night. At autopsy all three of the birds received at the laboratory showed a chronic proliferative enteritis, which was very severe in one of the three, the intestinal walls being greatly thickened. This condition extended from the gizzard almost to the rectum. Microscopical examination of scrapings of the mucous mem- brane and the contents of the intestinal lumen showed numerous nematodes. These were of two species (Heterakis vesicularis and Capillaria sp.). The former worm is normally a parasite of the caeca, and is said to.measure 7 to 13 mm. for the males, and 10 to 15 mm. for the females. In the case of the most badly affected chicken, however, the Heterakis worms were present throughout the entire length of the intestine. Further, while morphologically identical to H. vesicularis, they were small for this species. The males ranged from 4.0 to 5.4 mm. long, and a mature female, as evidenced by the presence of eggs, was but 7.2 mm. long. An immature female gave a length of 4.6 mm. While these worms probably aided in bringing about the con- 461 462 CLINICAL AND CASE REPORTS dition described, the greater blame must be placed on the Capil- laria. The female of this form varied from 11 to 13 mm. long and the eggs measured 45 to 25 microns. The males were some- what smaller. The genus Capillaria has recently been separated from Trichosomum, and is made up of worms the posterior ends of which are but slightly thicker than the anterior, whereas in Trichosomum the posterior ends are much thicker. All of these so-called whipworms have the habit of attaching themselves to the mucous membrane of the alimentary canal, the result of which is profound irritation and the secretion of a large quantity of mucus. In consequence the group is distinctly pathogenie. The literature doess not permit of a positive determination of the species of the worm found. Travassas (Memorias do Insti- tuto Oswaldo Cruz, vol. 7, pp. 146-172, 1917) gives two species of Capillaria as present in domesticated chickens, C. retusa and C. strumosa. These are separated according to the relative lengths of the narrow and broad portions. This, however, is not a particularly good character, for the worms taper rather regularly, and it is not at all easy to say where the narrow part ends and the broad part begins. This same author gives the following figures for the size of the eggs: C. retusa, 56-60 by 28-32 microns; C. strumosa, 60 by 25 microns. As already mentioned, the eggs of the worm under consid- eration measured 45 by 25 microns. It is possible, therefore, that it is a new species, but a more extended study than has yet been made would be necessary in order to determine -this point. The observation, however, is considered worthy of record, owing to the undoubted pathogenicity of the worm to chickens. It is probably also commoner than is generally supposed, since its position close up against the mucous membrane and embedded in mucus allows it to be overlooked unless a careful search is made. Dr. H. B. Wilkerson, of Bedford, Va., writes that he has had excellent results in the treatment of cerebrospinal meningitis in horses by the use of fluid extract of aconite. A dose of 5 minims per 100 pounds body weight is given every 3 hours. The treatment is continued until the symptoms of delirium dis- appear. ABSTRACTS THE So-CatLtep Borryomycosis iv Man. G. Romano. Tumori (Rome), vol. 8, p. 129, Aug. 10, 1921. (Abst. in Jour. Amer. Med. Assoc., vol. 77, p. 1139, Oct. 1, 1921.) Romano’s deductions from three cases personally observed and from the scanty literature on botryomycomas are that there is no identity between the disease in man and in horses Even those with the most experience are dubious whether to class the nodule as a neoplasm or a granuloma, and the term ‘‘human botryomycosis’’ is misleading. TUBERCULOSIS IMMUNIZING VAccINE. N. Raw. Brit. Med. Jour., 1921, No. 3147, p. 594. Abs. in Amer. Rev. of Tubere., vol. y (#921), *p. 119. Since 1906 human, bovine and avian tubercle bacilli have been subeultured by the author every month. The present growths represent the 184th generation. The growths are still luxuriant and true to type, but they do not produce tubercles and are completely nonpathogenic to animals. The pathogenicity has been tested every year. No change was noted until the 94th generation. After that time attenuation became marked, and after a year or two the cultures were avirulent. Treatment of disease by vaccines prepared from attenuated organisms nat- urally means that the production of antibodies will take place rapidly without any preliminary shock to the system, such as invariably follows the injection of vaccines prepared from viru- lent strains of bacteria. With a view to elucidating the real amount of tuberculosis in man caused by the human and bovine types of bacilli, and also as to immunity produced by one in- fection toward the other, Raw instituted clinical, observation on a large scale in a Liverpool hospital of 900 beds, of which he had charge for eighteen years. ‘In no case of primary pul- monary tuberculosis did he observe, during the course of the illness, tuberculosis of bones, joints, glands or skin, nor any instance of meningitis. Among the surgical cases of tuberculosis, 28 developed lung symptoms and 25 died. Im all cases the infection of the lung was either by direct extension from cervical lymph nodes to the apex of the lung, or from a primary focus 463 464 ABSTRACTS in the abdomen extending upward through the diaphragm to the bronchial nodes and lungs. This large clinical experiment convinced Raw that the human body is attacked by two quite distinet forms of tuberculosis: the one conveyed from person to person by direct infection and attacking chiefly the lungs, and the other conveyed by milk from tuberculous cows, and developed in the first few years of life. These two diseases are caused by different types of tubercle bacilli which will not live in the body at the same time; and one disease produces immunity to the other. Hence, Raw always uses a vaccine prepared from bovine cultures in the treatment of the human infections and vice versa. The vaccine which he has had prepared from his attenuated cultures is an emulsion of the bacilli, and contains all the prod- ucts of the bacillus. It is nontoxie and avirulent, and produces no reaction even in large doses. For purposes of immunization in susceptible children 6 injections are given at weekly inter- vals. This course is repeated in 3 months. The doses recom- mended are 0.001, 0.002, 0.003, 0.004, 0.005, 0.006 mgm. No symptoms have been observed to follow these injections. In the treatment of the active disease the doses are much larger than have been possible up to now. At least 12 injections should be given at intervals of seven days in doses increasing from 0.001 to a maximum of 0.025 mem. The vaccine should be freshly prepared from the cultures and should not be used after one month. It can be used with complete safety in any stage of the disease, and even in advanced cases great relief from night sweats and toxic symptoms have been noted. The results in suitable cases are excellent. Raw has succeeded in immunizing completely highly susceptible animals by the use of attenuated cultures, and he firmly believes that if all children with a tuber- culous history could be safely vaccinated in the manner de- scribed they would be placed in a better position to resist in- fection at home. RESEARCHES ON INFECTIOUS ABORTION IN Mares. M. Carpano. Ann. Ig. (Rome), 29 (1919), No. 11, pp. 752-779. Abs. in Exp. Stat. Rec., vol. 45 (1921), p. 179. The author reviews the literature on infectious abortion in mares and joint-ill or arthritis in foals and reports the results of his study of this disease in Italy, where abortions in mares, - ABSTRACTS 465 particularly the imported stock, are quite common and the foals born alive in localities where abortions have occurred are often affected with polyarthritis. Two microorganisms have been isolated from pathological eases, Bacillus abortus equi and a micrococcus of the type of Streptococcus equi, the former being considered the causative agent. The characteristics of these organisms are described and successful experiments are reported on the diagnosis of the disease by serum agglutination and on the therapeutic and pro- phylactic treatment of the disease with a mixed polyvalent serum. An Epizootic or Fown. TypHor mw FrRANceE. F. d’Herelle. Rev. Gén. Méd. Vét., 29 (1920), No. 339, pp. 128-130. Abs. in Exp. Stat. Ree., vol. 45 (1921), p. 181. The loss occasioned during a series of widespread outbreaks of disease among poultry in France was in a great majority of eases due to fowl typhoid (Bacterium sanguinarum), the exist- ence of which had not previously been reported in that country. It is said that in certain places there occurs in coexistence with fowl typhoid another disease of undetermined etiology which causes a high mortality among fowl and geese, the latter of which are not susceptible to fowl typhoid. IMMUNIZATION AGAINST BLACKLEG WITH GERM-FREE FILTRATES. E. Graub and W. Zschokke. Schweiz. Arch. Tierheilk., 62 (1920), Nos. 2, pp. 52-65; 3, pp. 112-122. Abs. in Exp. Stat. Ree., vol. 45 (1921), p. 180. From a series of experiments on the immunization of guinea pigs, sheep, and cattle against blackleg, the authors conclude that a single subcutaneous injection of blackleg filtrate produces an immunity which protects the animals against double the ordinary fatal dose of virulent blackleg material. A second inoculation with attenuated virus which is not fatal to normal animals, but in itself produces only slight immunity, greatly increases the immunity produced by the filtrate. It is con- sidered that the immunity is not only active and lasting against the strain used for the preparation of the filtrate but against other strains as well. 466 ABSTRACTS In practice, the single immunization with blackleg filtrate is recommended for cattle which are exposed to blackleg infection only during the pasture season and the double method for ant- mals continually exposed to infection. Inoculation with the virus should be given not earlier than 10 days nor later than two months after the filtrate inoculation. EXPERIMENTS CONCERNING THE ACTION OF COLD ON THE Bopy. D. Schade. Miinchen. Med. Wehnschr., 1919, p. 301. Regarding the influence of cold on the body, three effects are discernible: (1) The harmful action through colloidal tissue change in the cells. The injurious effects on the vitality of the cell consist in a change in the normal colloidal condition of the cell protoplasm, through which the colloidal Gepieplesa disap- pears (vacuolizing degeneration). (2) The remote effects of cold which are particularly reflex in character, proceed from the sympathetic nervous system (changes in the distribution of blood to the internal organs, muscular con- tractions, muscular flaccidity, anomaly of secretion of the in- ternal glands, for example, the kidneys). (3) Lowering of immunity. Experiments conducted on ani- mals exposed to intense cold out of doors, proved that such ani- mals possessed a lessened resistance to various infecting agents. J, P> Odirarye CONCERNING THE NATURE OF NECROTIZING PNEUMONIA IN SWINE PLAGUE AND Hoa CHouers. Muller and Sehmid. Miinchen. Tierarztl. Wehnscher., 1920, p. 274. The authors do not share in the view that swine plague has changed its nature in the course of the year and that the classical necrotizing form proceeds from the same cause as the chronic catarrhal form. They are of the opinion that the classical necro- tizing form of swine plague is by no means a particular infection, but rather the pectoral form of acute virus pest (hog cholera). ae mca ts A ls A i AID a a a ma ite ee ee -_ ARMY VETERINARY SERVICE THE PERCHERON AS AN ARMY HORSE Major Gen. Sir John Moore, Director of the British Army Veterinary Services in France, has written an excellent book on ‘‘Army Veterinary Service in War.’’? From this book the following paragraphs are taken: AMERICAN Heavy DrAuGHt Horses Though not so weighty and powerful as our best English heavy draughts, the American Percheron or crossed Percheron on the whole gave great satisfaction and was universally liked during the war in France. He teams well, is active, has a good constitution, is a good doer, and has good, sound legs and feet. The absence of hair on the legs was a great asset in comparison to our English heavies under the muddy circumstances of win- ter in the forward areas. He is best deseribed as a medium heavy, and as such he is quite big enough for the heavy draught purposes of war. As a draught animal I do not consider him in any way superior to our English Shire or Clydesdale, or to the smaller of these breeds of which he may be considered a parallel; but there is no doubt that in war he can be more gen- erally placed, and can stand hardships better than our heavy breeds. Whether or not his relatively satisfactory service in France was due in part to a return to the country of his ances- tors can only be surmised. He certainly stood the climate very well. He shipped to the country on the whole well, and suf- fered less from serious respiratory sickness on landing than the heavy Shires and Clydesdales. At the same time it must be remembered, as I have previously remarked, that respiratory sickness is. preferable to infection, and in all classes of animals incidents of this form of disease were very greatly reduced by the rigid taking of temperatures on landing and previous to drafting to units. Moreover, the heavy mortality experienced amongst Shires and Clydesdales was during the first winter of the war, when they were practically without shelter of any kind and subjected to incessant rain a very different state of affairs to the ample and good accommodation provided by such times as American shipments of heavy animals commenced. An idea of utility may be gathered from the records of one veterinary hospital in France, at which out of 120 heavy draught horses east and sold in two years, 116 were British (the 467 468 ARMY VETERINARY SERVICE officer commanding was unable to differentiate Clydesdale from Shire) and four only were American. In another veterinary hospital, a committee of officers, Royal Army Veterinary Corps, drew attention to the fact that a fairly large percentage of heavy draught American horses had side- bones, but expressed the opinion that this defect could soon be bred out by careful selection of sires; in like manner to its ex- clusion in our English breeds following a more particular classi- fication of the defect as an unsoundness. MULES In animal kind, the hero of the late World’s War—as in all other wars in which he has participated—that paragon of excel- lence, the mule, finds the premier place. He stands out promi- nently as a first-class war animal, and under all circumstances, in all climates or situations, whether amongst the mud of France, in the deserts of Egypt, on the plains of India, or on the hill- tops of the Himalayas, in burning heat or icy snow, his achieve- ments have been marvelous. He is as indispensable to war as a commander of the forces, and no history of war is complete without him. Any demerits he may possess are attributable to a psychology peculiarly his own, but his merits are double dis- tilled, and little more remains to be said on that account. AMERICAN MULES Of all countries in the world, none can surpass the United States of America for the production of mules, nor compete with it in general resources. The mule population of the States amounts to nearly 3,000,000, and the fountain never seems to run dry. During the South African war, the British govern- ment purchased 80,524, and though I have no actual figures to guide me, I should say purchases during the late war amounted to considerably over a quarter of a million. The strength of mules in the British Expeditionary Force, France, alone amounted to roughly 90,000. All sorts and sizes of mules are bred in the United States, from the small miner 12-3 or 13-0 to the magnificent sugar mule 16-2 and over. It pays better to breed a mule than a horse, and the market is for mining, lumber trade, and the cotton and sugar industries of the South. The real home of the American mule, and especially the large mule, is Missouri, though lighter mules are raised in Texas. If the magie names of Lathrop, Missouri and Kansas City are whispered into the long ear of ; bel f Army VETERINARY SERVICE 469 an American mule, he will immediately start a conversation about his old home, blue grass, Indian corn shucks and stover, his fine big mother, his French and Spanish ancestry on his father’s side, and he will air his views on stockyards and ‘‘nig- gers’’ generally. The American mule is wonderfully docile, and to my mind, quite the most handsome creature of the genus Equidae, and lovable withal. His power is best appreciated by standing close up to him: at a distance he may look mean. As a rider, a mule is of little value, a supreme will and an iron mouth, as a rule, prove the drawback. * * * Their happy nature goes a long way towards their success. They have a habit of worming their way into the hearts of our soldiers, and very soon friendly relations are es- tablished that work for the common good. Their endurance, their comparative freedom from sickness, their pluck and stout heartedness when properly treated, their ability to perform work under adverse circumstances and when short commons are necessitated, are their usual attributes; and their employment in war is a great economic factor. These remarks stand for all mules, whether American or otherwise. The majority of American mules employed in the various theatres of war were for light draught purposes, supplying the place of light draught horses in ammunition columns, etce., of formations, and receiying the same rations as light draught horses. To the latter factor the superiority of the mule over the light draught horse is greatly ascribed. If well fed, he thrives on work, and in times of idleness he will quickly get fat. As an instance of ability to stand the vicissitudes of campaign I will quote again the Somme operations in 1916. This offensive period resulted in 16,074 debility (poor condition) cases evac- uated to veterinary hospitals on lines of communications, of which total only 404 were mules. The percentage of inefficiency was 4.42 for horses and 0.61 for mules; horses suffered, therefore, seven times more than mules. During the winter seasons they gave us far less trouble than horsés (1 to 3, about) from skin disease, and respiratory disease was practically nil. These are very strong arguments in favor of the mule. I call to mind the limbered general service wagon mules of the 17th Lancers go- ing through the streets of Abbeville on a snowy day. They were pictures of health, and the bloom of their coats shone in spite of the snow. It is only one instance of many, and it made one feel proud to belong to our army. ASSOCIATION NEWS AMERICAN VETERINARY MEDICAL ASSOCIATION Proceedings of Fifty-Eighth Annual Meeting, Denver, Colo., September 5 to 9, 1921 (Continued from December JOURNAL) THURSDAY AFTERNOON, SEPTEMBER 8, 1921 GENERAL SESSION The meeting convened at 1:30 p. m., President David S. White presiding. Report OF AUDIT COMMITTEE PRESIDENT WHITE: The first item of business will be the re- ports of committees. Js the Audit Committee ready to report? (Dr. H. Preston Hoskins read the report of the Audit Com- mittee, as follows:) Your committee begs to report that we have examined the accounts of the Treasurer, Dr. Jacob, and a careful audit of his receipts and disbursements shows these to be correct. The books of the Secretary, Dr. Mayo, have received a very thor- ough audit at our hands, and the accounts have been found to be correct. The accounts of the JOURNAL, in the hands of the Editor, Dr. Mohler, were not audited by your committee, these having previously been examined by an accountant as provided by the Constitution and By-Laws. Your committee asks to be allowed to make several recommenda- tions: 1. There appears to be no valid reason for requiring the accounts of the Editor to be examined by a qualified accountant (see paragraph M of Section 7 of Article V of the Constitution), while this is not re- quired of the Secretary or the Treasurer. Article 17 of the By-Laws provides for an Auditing Committee, but attention is directed to the fact that the personnel of this committee may be completely changed from year to year, and a committee may not have a single experienced member on its personnel. The five mem- bers are usually widely distributed geographically and do not have an opportunity to examine the various books and accounts until the annual convention, when the time of the committeemen is often at a premium, a considerable amount of time being required for a careful audit. In view of the fact that the Association has a fiscal year, it would appear that the proposed audits might properly be made at the end of each fiscal year. 2. Your committee would also direct attention to the heavy financial loss being sustained at the present time by the Association, in con- nection with the remittance from Canadian members for initiation fees and dues. It would appear that this loss, in connection with the present difference in exchange, runs well over one hundred dollars per year. This loss could be naturally reduced, we believe, by having 470 Panis 0 sce he ttm ek _ y= a's PrRocEEDINGS A. V. M. A. 471 the Treasurer maintain a Canadian depository until such time as ex- change rates return to normal. Canadian banks are at present pay- ing 5% per cent interest on time deposits, and if necessary the funds on time deposit in the United States could be reduced an amount equal to that being kept on deposit in Canada. H. PRESTON HOSKINS, Chairman, R. H. MARSTELLER, H. E. BEmIs, Auditing Committee. (It was voted, on motion of Dr. Fitch, seconded by Dr. Hilton, that the report be received. ) Report oF EXECUTIVE BOARD PRESIDENT WHITE: Has the Executive Board a report te make? Dr. Hitron: At the last annual session this Association re- ferred to the Executive Board the question of considering whether or not the positions of Secretary, Editor and Business Manager should be combined. The Executive Board, in oraer to look into that matter very closely, appointed a subcommittee of the Board. This subcommittee has been looking into the question from every angle during the past year, and it has re- ported to the Executive Board, which has accepted the recom- mendations of the subcommittee. In looking over the situation with regard to the appointment of a permanent officer for these positions the Executive Board has also to consider our financial status. From the report of the Treasurer you will, note that we are only just balancing our- selves. We are going to draw on our capital account, and the Executive Board is ot the opinion that before a position of this kind is made permanent our capital account should pay sufficient revenue to pay a greater portion of our operation ex- penses. Our Treasurer has at the present time, as you will note in his report, money invested in bonds, also money on time deposit. We are endeavoring to get as much of that money invested in bonds as possible, so we can increase the interest on our principal. It is therefore recommended by the Executive Board that, in view of the facts which I have outlined, the officers be main- tained for the coming year as in the past, and the Executive Board therefore recommends that. the present incumbents of these positions, Dr. Mayo, the Secretary, and Dr. Mohler, the Editor and Business Manager of the JOURNAL, be maintained at the same remuneration as last year for the coming period. The Executive Board also wants to recommend an amend- ment to the Constitution, but I think it is very important that before this notice is given you clearly understand the object of the Executive Board in recommending this amendment. Under the present Constitution it is one of the duties of the Executive soard to recommend to this Association yearly the appoint- 472 Proceepines A. V. M. A. ment of a Secretary, Editor and Business Manager. In the work of the Executive Board during the past year it was found that so long as the Constitution required the Executive Board to recommend the appointment to these positions each year, and until the Association is in a position to make those posi- tions permanent, they will experience a great deal of difficulty in getting anyone to consider accepting such a position. We don’t know when the Association will be in a position to take such action. We are making an extra campaign during the coming season. We have organized to try to increase our mem- | bership. At the present time, before this meeting opened, we had but 4,000 active paying members. We have taken in 225 new members, but there have been some resignations, so we may reckon upon approximately 4,000 paying membership. We want to double that; we have to double that if we are going to extend and become permanent, and for that reason we are making a special effort to get new members. I thought it well to enlarge on that, so that no member of tnis Association would think that the Executive Board had something that it didn’t want you to understand. That is the only reason. Under the Constitution as it stands at present a person appointed to either of those positions or the combined position may be recommended yearly, but you ean be sure if he is an individual capable of filling those combined positions he naturally asks what are the chances regarding his perma- nency, and he sees the Constitution and sees that the Executive Board has to report every year and recommend individuals for those positions. The Executive Board therefore recommends that paragraph L of section 7 of the Constitution be amended by eliminating the word ‘‘yearly.’’ If the recommendation is accepted, it will have to be laid on the table until next year before it becomes operative. Dr. Eviason: As I understand, the proposed amendment was to be incorporated as read by Dr. Hilton, at this time. Dr. Hmron: The amendment is just to remove the word ‘‘vearly’’ from that section. Dr. Exiason: Wouldn’t it appear to you that there also might be a provision as to how long they are to remain in office? Where it is a definite appointment there ought to be some agency for removal. Dr. Hmron: There are. The Constitution provides that any of those individuals can be removed from office if neces- sary, provided it is recommended by the Executive Board and approved by the general session. PRESIDENT WHITE: This is simply received at this time and will be acted upon at the next meeting. Dr. JENSEN : It appears to me that this body has nothing what- ever to do with the secretaryship. No change could be made Proceepines A. V. M. A. 473 unless the Executive Board recommends it first, and then we can have a second whack at it. I am very much opposed to such a proposition as that. Dr. Cary: I move that the report be accepted and the amend- ment be referred to the Executive Board for action next vear. (The motion was seconded by Dr. Dimock and carried. ) SecrETARY Mayo: The following applications have been fa- vorably recommended by the Executive Board: C. B. Carpenter, Ithaca, N. Y. Pedro de Guia, Manila, P. I. J. B. Morales, Manila, P. I. Paulina M. Vytiacho, Manila, P. I. H. H. Sheeler, Buhl, Idaho. W. M. McLeod, Manhattan, Kans. Thomas Sims, Hutchinson, Kans. Stephen H. Hopkins, Kans. William Harvard, St. Augustine, Fla. The Board also recommends that the application of Dr. Her- minio Bernas, Iloilo, Philippine Islands, be accepted under sus- pension of rules. He is a graduate of San Francisco Veterinary College in the year 1918. The college has been dropped previ- _ ously, but as it has been out of existence, and is out of existence at this time, they recommend that his application be accepted. Dr. Hoskins: I move that the report of the Executive Board be received and that under suspension of the rules the appli- cants whose names have been read be admitted to membership by vote of the Association. (The motion was seconded by Dr. Dimock and carried.) Dr. ConNAway: I move that the rules be suspended and Dr. Bernas be elected to membership. (The motion was seconded by Dr. Hoskins and carried.) SECRETARY Mayo: The Executive Board recommends that Dr. J. W. Buchanan, of Union, S. C., be expelled from the Associa- tion for violation of the Code of Ethics. Dr. Hoskins: I move that the recommendation of the Execu- tive Board be received and approved by the Association. (The motion was seconded by Dr. Goss.) Dr. ConNAWAY: Some of the men around me seem to want to know what are the circumstances. We wouldn’t lke to cut a man’s head off without knowing the circumstances. SECRETARY Mayo: [ think it is right that they should know. A member from North Carolina sent in some advertisements from local papers of the Carolinas Remedies Company, of which Dr. Buchanan is the president, and said that they didn’t like it down in South Carolina. I wrote to Dr. Buchanan and told him that complaint had been made and that I hoped that he would discontinue this. I also wrote to the dean of the college from which he graduated, sending him copies of the advertisement, ATA. PROCEEDINGS A. V. M. A. and asking him if he couldn’t bring some pressure to bear on Dr. Buchanan to discontinue. We weren’t able to accomplish anything. ‘‘Satisfaction guaranteed,’’ and so on. It is glar- ing. After we couldn’t get him to discontinue it, charges were preferred by the Resident Secretary from that State, and Dr. Buchanan was notified to appear and defend himself against those charges. He replied, tendering his resignation to the Association. I notified him then that in my judgment the Exeeu- tive Board would not accept the resignation of a member against whom charges were pending, and that he should appear and defend himself against those charges, and he has made no answer to that. Dr. BELL: IT was a member of the Executive Board of the Southeastern States Association last winter, and Dr. Buchanan had an application in for membership, and we rejected him on those grounds. (The motion to accept the recommendation of the Executive 3oard that Dr. Buchanan be expelled from the Association was put to vote and was carried. ) SECRETARY Mayo: At the last meeting in Columbus the Ex- ecutive Board was authorized to appoint a Council on Chemistry and Pharmacy similar to that of the American Medical Associa- tion. The Executive Board has had this under consideration. during the past year, and it recommends that the Council on Chemistry and Pharmacy be made a permanent committee, con- sisting of five members, one to be appointed for one year, one for two years, one for three years, one for four years and one for five years—that is, at first—and that one shall go off this committee each year thereafter. Dr. STANGE: Wouldn’t that require an amendment to the Constitution and By-Laws? SECRETARY Mayo: I think it would. It hadn’t occurred to me before. ‘‘ There shall be the following standing committees con- sisting of five members except as otherwise provided.’’ It gives the five committees, but it would seem to me that they can be otherwise provided if the Association so desires. Dr. Cary: In construing that part of the By-Laws, that ‘‘otherwise provided’’ meant for other committees, not stand- ing committees. If you put this through, you have to have an amendment to the By-Laws. Therefore I move that this recom- mendation be offered as an amendment and referred to the Executive Board for report next year. (The motion was seconded by Dr. Adams and carried. ) SECRETARY Mayo: I have some telegrams I would like to read. T think the Association will be interested in them. I sent a message of greeting to Dr. Archibald, a member of the Executive Board from the Sixth District, who is ill. He says: ‘‘Thanks for the kind wishes, and I hope you have a pleas- ant and profitable meeting.”’ & a } PrRocEEDINGS A. V. M. A. 475 I also received a message from Dr. Blattenberg, Lima, Ohio: ‘““Why is mountain dew? Best wishes for good meeting.”’ (Laughter. ) Report OF COMMITTEE ON INTELLIGENCE AND EDUCATION PRESDENT WHiITE: Is the Committee on Intelligence and Edueation ready to report? (Dr. Bergman read the report of the Committee on Intelli- gence and Education, as follows:) During the past year twelve of the fourteen veterinary colleges in the United States and Canada approved by this Association have been visited by members of this committee, and in addition one college, the Georgia State College of Agriculture, Division of Veterinary Medi- cine, which is asking recognition this year as an approved institution. With two exceptions these institutions were visited while school was in session. In all cases the visiting members of the committee were courteously received, every assistance being given in carrying out the inspection and all requested information relative to entrance require- ments, curricula, faculties, teaching methods, equipment, etc., freely furnished. Data secured by the committee indicate that all veterinary colleges at present recognized by the Association are complying with the regu- lations relative to entrance requirements, length of curriculum and transfer of students. Relative to the latter, the Secretary has from time to time referred correspondence to the committee from various deans relative to questions of transfer of students from certain of the nonaccredited schools. Questions relative to the interpretation and enforcement of entrance requirements, status of Federal Board students and certain individual cases needing special consideration have been referred by various deans to the committee for suggestions, the attitude in all cases being to comply with the suggestions made. Investigations of the various colleges this year showed, in a num- ber of institutions, a marked improvement in gener al condition over last year, improvements varying from additional buildings and equip- ment to improvement of faculty personnel and general instructional methods. Several institutions have secured within the last year mod- ern buildings which were badly needed, the aggregate value of which will total into several hundreds of thousands of dollars. The com- mittee believes that it is not breaking faith in stating that the A. V. M. A. through its Committee on Intelligence and Education has by correspondence, recommendations and personal conference with college authorities been of considerable assistance to the deans in securing many of. the above-mentioned improvements. Developments of the past year would seem to indicate that close cooperation between this committee and the deans of the various veterinary colleges can be made of considerable mutual benefit in securing needed improvements at the various schools, working ultimately for the betterment of the veterinary educational situation, which is the aim of the Association. The future standing of the veterinary profession depends upon the educational standards it maintains. The foundation of the profes- sion is its education. If the veterinary profession can not, or does not, maintain educational standards equivalent to those of other pro- fessions, then it can not expect to become permanently established on an equal plane with them. It is the earnest desire of the committee to cooperate with the va- rious veterinary colleges for the betterment of veterinary education. The attitude of the committee should be and is a cooperative and helpful one rather than strictly critical. It is not the intention to 476 Proceepincs A. V. M. A. injure or work a hardship on any educational institution by excessive demands, but rather request a reasonable standard of educational efficiency. As has been demonstrated in the past, the prestige of the A. V. M. A. can be of considerable assistance to the various schools in securing needed improvements. During the past year the committee has had two meetings in Chi- cago, the first at the time of the Live Stock Sanitary Association meeting, to discuss the recommendations of the committee as ap- proved at the Columbus meeting, hear subcommittee reports and out- line future plans, and the second following the inspections of the va- rious schools to discuss the general educational situation, outline sug- gestions to the various schools and plan the committee report for this year. Criticisms and suggestions which the committee believes of a constructive nature have been forwarded to the deans of various in- stitutions and for the most part have been acknowledged and favor- ably received. Considerable comment has been made in the veterinary press gen- erally, the past two or three years, relative to the decrease in num- ber of students attending veterinary colleges since the year 1916. The following tabulation shows a total of 870 students matriculated for the session 1920-21 in the fifteen veterinary colleges accredited by this Association. Of this number 187 were freshmen. In addition there were approximately 120 students. attending colleges not ac- credited, or a total of 990 attending the veterinary colleges in the United States and Canada. This is approximately one-third the num- ber attending the 23 veterinary colleges existing in 1916. Numerous factors are responsible for this decline, the principal one undoubtedly being the increase in entrance requirements and length of curriculum. There are those who have become so alarmed over a shortage of veterinarians that they have advised lowering en- trance requirements and the veterinary colleges again accepting any one unable to enter the other learned professions. This is certainly not the remedy. The remedy lies rather in increased publicity regard- ing the profession’s attainments, better remuneration for service, bet- ter appropriations for veterinary colleges and veterinary research whereby veterinary education may assume a position on a par with the other professional and technical lines of work and be properly regarded and appreciated by the public. Our worthy president, Dr. White, summed up the situation in a few remarks in a recent address when he stated as follows: “We are already far enough behind the entrance requirements of the other professions. We can not make progress by walking back- wards. In the final outcome the matter will adjust itself. If in the next few years the number entering the profession is too small to meet the demand, the public will recognize the fact and we will again find our veterinary institutions filled.” Last year the Association approved a recommendation of this com- mittee that it be empowered to outline what might be regarded as the “essentials of an approved veterinary college’”’ and also a schedule whereby veterinary colleges might be graded and, if desirable, classi- fied, and after submitting these to the Executive Board that the two bodies inaugurate the use of the same for the inspection of veterinary colleges during the year 1920-1921. In working out the suggested out- lines the committee studied closely the general plan used by the Council on Medical Education of the American Medical Association and secured valuable cooperation from the secretary, Dr. N. P. Colwell. The mat- ter of a schedule for vrading and classifying veterinary colleges has been given considerable study by this committee; however, no plan is suggested or recommendations made at this time. The matter needs Proceepines A. V. M. A. 477 STUDENT ATTENDANCE AT APPROVED VETERINARY COLLEGES, SESSION 1920-21. Name of Institution First Second] Third | Fourth Special Year | Year Year | Year | Alabama Polytechnic Institute....................... | 20 15 12 6 Colorado Agricultural College............-...-....... 26 22 20 | 20 Georgia State Agricultural College!............0...... 1 eas 3 4 incumnsy etermibry Gollege. 5.32.5... 25-1. eon cee 1l 28 23 73 1 LTD SCTE D EE ee ia ae ae 30 24 18 22 Kansas State Agricultural College...........-......-. 9 20 13 18 1 Michigan Agricultural College.......__. ae me 1 8 4 8 Universite de Montreal.........._... oops anc, Seer 12 4 4 6 New York State Veterinary College (Cornell)........ . 13 32 15 16 2 New York State Veterinary College (New York UDRP ESER ED LOR Se Se 7 3 5 8 - Ohio State University... ... i ee ee ee 19 30 30 25 Ontario Veterinary College (Toronto, Canada)........ 28 32 22 17 Ainiwersitqor Penns yivania.. 5050. cnc. cee eee 4 8 qe il Texas Agricultural and Mechanical College............ peal 5 2 6 4 State College of Washington......................--. 3 1 6 12 Shs Se eae ae | 187 | 239 | 184 | 252 | 8 1 Recommended for approval 1921. further study, and it is probable that the cooperation of the deans of the various colleges will be requested at a future time to assist in the outlining of such a schedule. The following tentative outline of the essentials of an approved veterinary college has been pre- pared by this committee to serve as an aid in the inspection of vet- erinary colleges: ESSENTIALS OF AN APPROVED VETERINARY COLLEGE Entrance Requirements 1. The matriculation requirement of an approved veterinary college shall be not less than four years of high-school work or equivalent studies taken in other preparatory schools, provided that candidates for admission to approved veterinary schools who can not present a satisfactory certificate from the proper official of the school or schools attended covering the required amount of preparatory work may be admitted upon passing satisfactory examinations approved by the proper State educational officers. Administration of Entrance Requirements 2. The admission of students to the veterinary school must be in the hands of a responsible committee or examiner, whose records shall always be open for inspection. Documentary evidence of the student’s preliminary education should be obtained and kept on file. When the veterinary school is an integral part of a university, this work usually devolves on the university examiner. Unless the uni- versity examiner and his records are closely accessible, however, some officer at the veterinary school should obtain and keep on file docu- mentary evidence of each student’s preliminary education, including both high-school and collegiate work. Advanced Standing 83. (a) Full advanced standing may be granted to students only for work done in other approved veterinary schools, and in granting advanced standing official verification of the student’s previous vet- erinary work should be obtained by direct correspondence with the college previously attended, and his preliminary qualifications should also be verified and recorded the same as for freshman students. (b) Advanced standing granted to students for work done in non- approved veterinary colleges should be strictly on the basis of exam- 478 PrRocEEDINGS A. V. M. A. inations in the particular subjects in which credit is desired. As in the case of students seeking full advanced standing, official verifica- tion of the student’s previous veterinary work should be obtained and his preliminary qualifications should be verified and recorded. Attendance (a) The college should require that students be in actual at- tendance in the college within the first ten days of each session and thereafter. (b) Actual attendance at classes should be insisted on except for good cause, such as for sickness, and no credit should be given for any course where the attendance has been less than 80 per cent of the full time. Supervision 5. (a) There should be careful and itrellioent: supervision of the entire school by the dean or other executive officer who holds and has sufficient authority to carry out fair ideals of veterinary education as determined by the present-day knowledge of veterinary medicine. (b) There should be a good system of records showing conveniently and in detail the credentials, attendance and grades of the students, by means of which an exact knowledge can be obtained regarding each student’s work. Records should also be kept showing readily the attendance of students at hospital and ambulatory clinics. Faculty 6. (a) The college should provide at least five thoroughly trained veterinarians in charge of major departments, salaried so that they may devote their entire time to instruction and to that research with- out which they can not well keep up with the rapid progress being made in their subjects. A sufficient number of assistant veterinarians should be provided to conduct the work properly, the number of the latter being governed by the number of students and amount of re- search work being conducted. In no case should a head of department be a veterinarian of less than five years’ experience in his particular major subjects. (b) The faculty should be made up of graduates of recognized vet- erinary colleges. Other than veterinarians should be selected as teach- ers of veterinary subjects only under exceptional circumstances and only when veterinarians of equal special capacity are not available. The faculty should be thoroughly organized with an executive head or dean and each department having its head professor directly re- sponsible for all instruction given in his particular department. Curriculum 7. (a) The college curriculum should be fully graded and extend over four collegiate years of not less than thirty-two weeks each and of not less than seventeen credit hours per week, exclusive of time required for matriculation and holidays. The courses offered in the various subjects should be set forth in the annual announcement, so as to show for each course its number, subject, content, character (lecture, recitation, laboratory or clinic), length of time devoted to each and the amount of credit allowed. The courses for each class should also be clearly set forth in a class schedule, for the guidance of the students. (b) The college should give two years of work consisting largely. of laboratory work in well-equipped laboratories of anatomy, histol- ogy, pathology, embryologv, physiology, chemistry, bacteriology, phar- macology, therapeutics and clinical diagnosis. In addition selected animal husbandry subjects, such as breed types and judging of live- stock, animal breeding and feeds and feeding, should be required. prea eontn : pala My a : Proceepinecs A. V. M. A. 479 Present-day knowledge makes it essential that these subjects be in charge of full-time, well-trained teachers. (c) Two years of clinical work with courses in medicine, surgery (including surgical anatomy and operative surgery on experimental subjects), obstetrics, serum therapy, parasitology, sanitation, food hygiene and necroscopy. General Teaching Facilities and Instruction 8. (a) The college should show evidences of thorough and reason- ably modern instructional methods in all departments. Adequate class- room and laboratory facilities and such equipment as is generally regarded as being essential to the teaching of the various laboratory branches should be provided. Evidences should be shown that student work is being carefully supervised and that equipment and facilities are being intelligently used in the training of veterinary students. (b) Each college should be supplied with such auxiliary equipment as projection and photographic apparatus, charts and other apparatus now so generally used in medical teaching. (c) Modern experimental laboratory work in physiology, pharma- cology, bacteriology, as well as for medical research, necessitates a supply of animals such as frogs, rabbits, guinea-pigs, etc., and proper provision is necessary for the housing and care of such animals. (d) There should be sufficient embalmed material to enable each student to dissect the lateral half of the horse, ox, pig, sheep, dog, cat and fowl and to provide cross sections, special dissections and other demonstration material. Care should be used to provide material from both sexes of each species for simultaneous dissections and demonstrations. Pregnant and immature subjects for dissection are also helpful. In the case of the horse, at least, a living animal should be in the dissecting room for a “palpator.” (e) The college should have a working library, to include the more modern veterinary text and reference books together with related scientific text-books and journal files. The library should receive regu- larly the leading veterinary and related scientific periodicals, the cur- rent numbers of which should be in racks or on tables easily acces- sible to the students. At the end of each year these periodicals should be bound and added to the files of bound periodicals. The library room should be properly lighted and heated, and open during all or the greater part of the day. (f) There should be a working museum having its various anatom- ical, surgical, obstetrical, pathological and other specimens carefully prepared, labeled and indexed so that any specimen may be easily found and employed for teaching purposes. It is suggested that so far as possible with each pathologic specimen coming from post- mortems there also be kept the record of the postmortem, the clinical history of the patient on which the postmortem was held and micro- scopic slides showing the minute structures of the diseases shown in the gross specimen. The museum furnishes an excellent means of correlating the work of the department of pathology with that of the clinical departments. Clinical Facilities and Instruction 9. (a) The college should be provided with a clinic building or clinic rooms with facilities for receiving, recording, examining and treating medical and surgical cases of all species of animals. The building should contain a dispensary with drugs, instruments and instrument sterilizers; one or more operating rooms with operating tables for large and small animals; a clinic room for the examination and daily treatment of patients, and stalls and kennels for hospital patients. (b) An average of not less than 100 cases per month, well dis- 480 Proceepineas A. V. M. A. tributed as to species, is desirable for senior classes of twenty-five students or less, these cases to be of such character as to permit the students to see and study the common variety of surgical and med- ical cases in each species. (c) Clinical instruction should be offered to junior and senior stu- dents. The same cases may be used for both classes, each case being assigned to a senior student having one or more junior assistants. Each student group should be responsible for making a detailed ex- amination of the patient assigned, assisting in the treatment, and making careful records of the case until it is discharged from the hospital. Where clinical material is limited the instructors should see that each student in the class has knowledge of each and every case, and students should be required to pass examinations in their clinical work as well as any other subject. (d) Facilities should be provided for proper postmortems on ani- mals dying in the clinics during each college session, which should be attended by both classes and participated in by senior students. These should be performed in cooperation with the professor of pathology. (e) The clinical staff should include the professors of medicine and surgery and their assistants, and they should have the close coopera- tion of the professors of pathology and bacteriology. The examina- tion of patients, the preparation of drugs and instruments should be under the close supervision of the instructors, and all treatment and operations should be done by the instructors assisted by the students assigned to the case. Attendance at the clinic should be prompt and regular on the part of students and instructors. ‘(f) An ambulatory clinic should be maintained for senior students under the direction of a good practitioner and instructor who is a regular member of the faculty. Students should be assigned to this work in small groups for definite periods, and the members of each group should go on all calls during their periods of assignment. 19. A clear statement of the college’s requirements for admission, tuition, sessions, courses offered and graduation should be clearly set forth in regular annual catalogs or announcements. In visiting the various colleges this year your committee has en- deavored to apply the above outline as a basis for judging the work of the various institutions, and it has proven of material assistance in securing a greater degree of uniformity in the school inspections. No institution visited presented, of course, a condition of 100 per cent efficiency. As previously stated, certain institutions showed a marked improvement over their 1919-20 condition and have en- deavored to carry out as far as possible previous suggestions and recommendations of this committee. Last year at the Columbus meeting this Association approved of the principle of a publicity campaign the aim of which was to bring before the public some of the important phases of veterinary medicine in reference to the relation of the profession to public health, ad- vancement in education, field and scope of work and its general rela- tion to society. However, after careful consideration by this com- mittee, in addition to conferences with the Executive Board, it was not deemed advisable, on account of lack of funds, to begin publicity work of this nature the past year. Recently the subject has again been brought to the attention of the profession through the editorial columns of one of our veterinary journals, wherein comment and sug- gestions are presented in connection with a discussion of a “News Service for Science” recently established in Washington, D. C., under the name of “Science Service.” Science Service is the name of an agency newly established in Washington through the generosity of bs g Proceepincs A. V. M. A. 481 Mr. Edward Scripps of Miramar, Calif., for the diffusion of knowl- edge regarding science. The governing board of fifteen trustees is composed of representatives of the National Academy of Sciences, the American Association for the Advancement of Science, the National Research Council, the Scripps Estate, and the journalistic profession. Its function is to serve all sciences, acting, as it were, as an ethical agent between scientific circles and the outside world, and through popular science writings instruct the people as a whole regarding the aims and achievements of the various branches of modern science. It would seem that this service might afford the means of giving to veterinary science and the veterinary profession a wider and more desirable publicity, and is worthy of investigation by this Association. Summary 1. This committee recommends the following list of veterinary col- leges as approved colleges for the ensuing year: Alabama Polytechnic Institute, College of Veterinary Medicine. Colorado State College, Division of Veterinary Medicine. Georgia State College of Agriculture, Division of Veterinary Medi- cine. Indiana Veterinary College. Iowa State College, Division of Veterinary Medicine. Kansas State Agricultural College, Division of Veterinary Medicine. L’Ecole de Médecine Vétérinaire, Université de Montreal. Michigan Agricultural College, Division of Veterinary Science. New York State Veterinary College at Cornell University. New York State Veterinary College at New York University. Ohio State University, College of Veterinary Medicine. Ontario Veterinary College, Toronto University. State College of Washington, College of Veterinary Science. Texas Agricultural and Mechanical College, School of Veterinary Medicine. University of Pennsylvania, School of Veterinary Medicine. 2. This committee recommends that the “Essentials of an Approved Veterinary College” as herewith submitted be approved. 3. This committee recommends that this Association, through an authorized committee, investigate the newly organized “Science Serv- ice” as herein mentioned, and if advisable endeavor to have such edu- cational publicity articles prepared as may properly give the pro- fession wider publicity, and, following approval of the committee, be placed at the disposal of this science news agency for publication. 4. This committee recommends that, in order that sufficient funds may be available for the work of the Committee on Intelligence and Education to carry out properly its projected work, the Budget Com- mittee, if it deems advisable, increase the appropriation for the work of this committee from $1,000 to $1,500 for the ensuing year. Cassius WAY, Chairman (Term expires 1922), L. ENos DAy (Term expires 1921), L. W. Goss (Term expires 1925), B. T. SIMMS (Term expires 1924), H. D. BERGMAN, Secretary (Term expires 1923), Gamnisitee on Intelligence and Education. 482 PROCEEDINGS A. V. M. A. PRESIDENT WuiTE: You have heard the very excellent report ot the Committee on Intelligence and Edueation. What shall be done with it? Dr. Sisson: I move that the report be adopted. (The motion was seconded by Dr. Hoskins. ) Dr. EicHHORN: There is one recommendation with regard to the heads of departments which I think ought to be amended. Tt I understand correctly, the statement was that the head of any department should require five years’ experience in that particular branch. It seems to me that sometimes it might work to the disadvantage of a particular college if you adopt that. Have the time limit stated definitely. It might be that the professor in some veterinary college might be suited if there is a vacancy in some other department. If we adopt this, I don’t think this could be accomplished, so I move that the com- mittee change this particular clause. PRESIDENT WHITE: Is this clause mandatory, Dr. Bergman ? Dr. BERGMAN: It says ‘‘in no case should a department,’’ ete. It is not mandatory. PRESIDENT WHITE: The motion to accept the report is before you. Dr. R. C. Moore: I would like to offer an amendment to the report: That the St. Joseph Veterinary College be considered in the accredited list of this Association. (The motion was seconded by Dr. Conrad.) rR. BERGMAN: If I understand Dr. Moore’s motion correctly, it is that the St. Joseph Veterinary College be added to the list of approved veterinary colleges. This would seem to be a very extraordinary request on the part of Dr. Moore, as this com- mittee has no information as to the condition of this veterinary college during the past year. St. Joseph Veterinary College was discredited at the New Orleans meeting and dsked for re- examination the next year, and the committee, following the re- examination, recommended at Columbus that it remain on the diseredited list. It does not seem that we should place on the approved list a college regarding the present condition of which we have no information. Dr. R. C. Moore: I would like to explain the situation. Last year, When we were rejected at Columbus, a motion was made immediately asking that our institution be inspected again for future consideration. During the discussion on that question the Chair made the statement that it was unnecessary to adopt such a motion because our college would be regularly inspected in the rounds of inspections. We have depended upon that state- ment. We have waited day after day, throughout the year, expecting from time to time that this committee would visit us and inspect the institution. I even waited at home until the last train before I could reach this convention, thinking they might stop there on their way to the meeting, as most of them ProcEEpINGS A. V. M. A. 483 lived beyond that point. They did not come. The facet that it has not been inspected is no fault of ours. We are meeting every requirement as read here today by the chairman of this committee. We have put in last year our full time as we did the year before, of thirty-two weeks of actual teaching time. We have taught every subject that is required. We have taught it as thoroughly, I believe, as any other school is doing. Our clinics have been good. In fact, the men who have graduated from, that institution in the last year, I believe, particularly have demonstrated this fact, and they have passed your State boards wherever they have gone. Not a single failure has been reported this year. We graduated thirty-seven men. We had a hundred students, and we did not admit a single man except on the high-school requirements as required by this Association, and we do not see why we should be discriminated against, and not be allowed the privilege of other members of this Association. The faculty ‘and members of that institution are members of this Associa- tion. We have been members of this Association for a good many years. We have done our part as best we could for the upbuilding of this Association. We believe we have no right to be discriminated against because of no failure on our part. We ask to be reinstated to the list of accredited colleges, and if we are found to be not living up to these conditions, we are willing to take the consequences. I ask you, gentlemen, to con- sider this carefully and not do an act that will ruin an institution that has labored year after year, and that will ruin the men at the head of the institution who had labored year after year for the upbuilding of the institutions of the country. I ask you to consider this carefully and vote as you think is right. If we are discredited this year it means another hardship to maintain a sufficient number of students to meet our expenses. We did so last year, notwithstanding the fact that we received a stunning blow, and accusations were made against us, that somehow or other came to the committee, that were not correct. I want to say one thing while I am on the floor. I am not saying this to discredit the committee, but to prove what I said. The statement was made at Columbus (that was one of the contentions of the committee) that we had admitted men to advaneed standing who should not have been admitted. The chairman of the committee made the statement that we had ad- mitted Edward Trenton Prends, who had come to us with credit for two years’ work in the Iowa College, with a certificate on which was written ‘‘disqualified.”’ I didn’t believe it at the time. The man had been an elegant student, and the committee went on to say that this man was not bright. Where they got their information I do not know. I have in my pocket a photo- graph of that certificate. It is here for your inspection. Not a single line of any description is written across its face. Down 484 Procrepines A. V. M. A. in the corner, however, is written: ‘‘Letter of honorable dis- missal her eunto attached. is I have the letter here, which reads as follows: ‘“To Whom It May Concern: This is to certify that Edward Trenton Prends is this day at his own request granted this tes- timony of honorable dismissal, September 14, 1918.’’ It is signed by the registrar. They didn’t stop with that. It seemed that they liked him. ‘‘Mr. Trenton Prends, Moulton, Iowa. My dear Mr. Prends: Replying to yours of the Ist instant, we are inclosing herewith certificate of your credit with letter of honorable dismissal. We are sorry to lose you from our student enrollment and hope you will enjoy your work wherever you decide to register, and that you W ill return to Towa State College later to finish your course.’ I admitted this man on these credentials, and I believe I did right. I believe they are as clear as can be, and I am read- ing you this to show you that this committee has been mis- informed from some source or other. I believe it was an injustice to this man to make a statement of this kind. That is the reason TI am reading this and showing these documents here for your consideration: I again ask you to give this your careful consideration. Dr. BeraMan: Mr. Chairman, one might think from the lat- ter part of Dr. Moore’s discussion that his institution was re- fused recognition because of this particular case (Trenton Prends). If this committee made a mistake in the examination of the credentials of one student, that would not be remarkable, because when you go into an institution and commence to ex- amine the credentials of the students, it is quite a complicated task. At the New Orleans meeting the criticism particularly emphasized was the matter of matriculation requirements, be- cause at the meeting in New Orleans, when the St. Joseph School was first discredited, it had failed to meet the matriculation re- quirements laid down by the Association relative to four years’ high school, and there was no use of further discussion. Neither had the requirements been met at the time of the Columbus meeting, for the school had not yet opened for the season of 1920-21. In addition, however, the objections of the committee to the St. Joseph School went further—namely, that there was a lack of proper facilities to carry out efficient veterinary educational work. So far as this individual instance that Dr. Moore brings up is concerned, if the committee owes an apology for that, all well and good. Don’t think, however, that this individual instance was responsible for the diserediting of the St. Joseph Veterinary College. The committee recommended that the St. Joseph Veterinary College be discredited largely in view of the fact that in the opinion of the committee it was not carry- PrRocEEDINGS A. V. M. A. 485 ing out efficient educational methods and did not have the fa- cilities to do so. Relative to the inspection of the St. Joseph College this year, if the committee had had any idea that it desired inspection, it would have been given. The committee was not requested to inspect the college by the college authorities, neither was it in- structed by the Association to inspect the college. The committee discussed the case of the St. Joseph Veterinary College at the meeting in Chicago in December, and it was agreed that inasmuch as we had not been requested to do so, we would not inspect the college. Do you suppose for a minute that this committee would walk into an institution over which the Asso- ciation has no jurisdiction and inspect it without being re- quested to do so? If we had been requested to inspect the St. Joseph Veterinary College we would have done so. As a mat- ter of fact we heard reports to the effeet that it was immaterial to the college whether it was recognized by this Association or not. We sincerely hope, as the committee is directly responsible, that this Association will not see fit to approve this college without definite information as to its present condition and educational methods being employed. Dr. Canim: Last year we asked for a committee to investigate this college at once that we might be put upon the accredited list. The Secretary at that time made the remark that he would fight with every power available the appointment of a special committee or a visitation of the regular committee to this school at that time, saying at the time-that this would be investigated and looked after during the regular visitations. Dr. Cary in his remarks said that this school would be visited by the regu- lar committee during the course of the year. We not only asked for this committee to investigate us, but we tried to get a special committee or this committee to make a special trip to investigate us, and did not succeed. It seems strange to me that in the face of that evidence this committee will come before you here and say that they had not received an application from this college to visit it. During the year we have endeavored to keep up the high standard of the school as it has been in the past. The statement was made that the college was lacking in certain essentials. I would refer the Doctor to the previous report of the previous committee, which complimented us upon our laboratory facilities and the manner in which the work was conducted. No change had been made during the time. It is strange that two com- mittees would differ to that extent. We waited until last Sunday for this committee. It is no fault of ours. If this convention thinks it is fair to this school that we should stay on the discredited list for another year on account of a mistake of this committee, it is up to vou. We ask for nothing but justice. (Applause.) 486 Proceepines A. V. M. A. Dr. Conrad: It has been my pleasure and privilege to be in close proximity to this college. I live a short distance out of this city. I will state plainly I never had any request to visit this college. I go to St. Joseph frequently, and invariably loiter around and visit the college and visit the classes. I am a mem- ber of the State Veterinary Board in Kansas, having been ap- pointed this last year. I looked over the papers of the graduate members of the class, and I must say, in highest regard, they passed better examinations than I ever had the privilege of read- ing from any college or any institution. I am not saying this on account of any friendship I have for the college, the professors, or anything of that sort. This talk I give you comes unsolicited. I talked with Dr. Luckey, of the Examining Board of Missouri, and every man passed the examination. I had the opportunity of perusing some of the questions asked this class, and I will state that it was a very severe examination. They gave a real examination. We gave one entirely different, but covered the subjects in completeness, and the men from St. Joseph passed a very creditable examina- tion. Dr. Wolfe was president of the board, and he said, ‘‘We have to give St. Joseph a handeclap for the manner of their edu- cating their students in this college.’’? (Applause.) Dr. R. C. Moore: I would like to call attention of the Associa- tion to the fact that Dr. Bergman seems to think it would be improper to accredit this college without the recommendation of the Committee on Intelligence and Education. I just want to remark that we were discredited in New Orleans in face of the fact that the committee not only recommended us favorably, but the chairman of the committee stood before the Association and pleaded for our continuance on that list. Yet you took it out of the committee’s hands and voted us out. Is it any more harm to vote us in without the recommendation of the com- mittee than it was to vote us out? (Applause.) Dr. GRAEFE: I happened to be present at the Columbus meet- ing last vear, and think I understand the proceedings in refer- ence to this question. I would take it to mean that that institu- ion at that time was promised consideration and inspection dur- ing the ensuing year. I have been in the office of the St. Joseph Veterinary College once. I have never been through the institu- tion, and know nothing about their equipment or any of the other circumstances in question, but it does seem to me like an injustice that when these matters are brought so forcibly before this Association, the Committee on Intelligence and Education did not take the means to be able to know something about this institution when this question was brought up at this time. I don’t know what the Constitution and By-Laws say in ref- erence to that matter, but it seems to me if we want to conduct efficiently the aggressiveness of veterinary education in this country, when there is one institution in question, as this one Proceepincs A. V. M. A. 487 has been, that the mere matter of the formal request, whether given or not, should not be justification for keeping this institu- tion on or off the accredited list, if it is entitled to be on it. It seems to me as though it is a matter of justice and justice only, and one which, if not given at this time, is going to delay that institution another year, which is a very vital factor in its man- agement. I don’t want to be responsible for any sentiments to lower the educational standards, but I do feel that the inter- est of the veterinary profession in this country demands that every institution in this country be given justice. (Applause. ) Dr. Sims: As a member of the Committee on Intelligence and Edueation I beg to call attention to the happenings at Columbus last year. The St. Joseph Veterinary College did not request on the floor at Columbus that we inspect the institution this year, but where the request came in was in a motion from Dr. Koen, asking that the Committee be instructed to inspect, with power to put the institution back on the list of accredited col- leges without referring back to this Association. The request was a motion asking that our committee be given power to act. The motion was lost, Dr. Mayo stating, as has been said, that he would fight very much any motion or movement to give the committee power to act without referring back to the Association as a whole. That was the only request that we received, that request coming, as I say, from Dr. Koen in form of a motion, which the house as a whole voted down. There has been some little discussion of what took place there, and I wanted to eall attention of all the members present to what happened there. I have looked up the minutes of the previous meeting. (Ap- plause. ) Dr. P. A. FisH: There seems to be a question of veracity in- volved, and that, perhaps, can not be settled at this meeting. I was at the New Orleans meeting and remember when this ques- tion came up for discussion. The requirements for the ac- credited list were published in advance, and the private schools had a meeting to talk over the future and discuss whether or not they should. comply with these requirements. As a result of that meeting certain of the schools did comply with the require- ments and were recognized and accepted by the Association. The St. Joseph School did not comply with the requirements at that time, and yet a very earnest plea was put up at the New Orleans meeting for it to be taken in on the accredited list just the same. That brought up the question of fair play. Is it right to penalize an institution that did comply with the requirements, although much hardship was involved, and overlook the negli- gence or possibly the defiance of another school that failed to take the proper action? I think that was the question at issue. Nobody who intends to do justice in connection with this matter would be willing to take this school into the Association without any examination. The committee has been appointed for the 488 Procerpines A. V. M. A. very purpose ‘ol looking over the equipment of the schools. It is their function to know about these things, and their opinion is more valuable to the Association than that of parties directly interested in the school, because their opinions are naturally more or less biased. I think the Association should stand by its committee. I think we should be guided very largely by what they have to say. It is unfortunate that the committee has not been asked to visit the school before this time. It is difficult for the layman in the Association to judge, but the issue now seems to be whether the school shall come into the Association on the state- ment of its dean or whether it shall come in in the proper way, by recommendation of the committee. Dr. Conran: In the face of the remarks the gentleman just made, I would offer an amendment to the amendment, which is contrary to the motion, that three additional members be added to the committee, to make immediate investigation of the St. Joseph Veterinary College, and if their findings are such that it is an aceredited college, that it be immediately published and recommended. (The motion was seconded by Dr. Connaway.) Dr. STANGE: I rise to a point of order. I think that amend- ment is unconstitutional. The Constitution provides for the Committee on Intelligence and Education and its personnel. Any committee that would be appointed to investigate this in- stitution would have to be a special committee or a committee to cooperate with this committee. PRESIDENT WuitTE: The Chair sustains the point or order. Dr. Conrap: I make a motion to amend the amendment, that the Committee on Intelligence and Edueation be asked to inspect immediately the institution and immediately report to the Asso- ciation. (Seconded. ) Dr. BERGMAN: This committee is to report to the Association annually. We can not report until the next annual meeting of this Association. We shall be very glad to make the inspection upon request. PRESIDENT WuiITE: Is there any further discussion ? Dr. Sisson: It seems to me this last motion is unnecessary. It can’t be amended. Dr. Conrap: In view of the fact that you give complete power and usually do according to the recommendation of the com- mittee, it seems to me that we have held this in suspension long enough; that this college should be either recognized or dis- qualified forever, and it is a discrimination to keep this college out if it is complying with the requirements which establish a good college. We should act in some manner according to tech- nicality of some kind. This college deserves looking into. PRESIDENT WHITE: An amendment to an amendment must be germane. The Chair will rule the amendment to the amend- . | } ProcEepincs A. V. M. A. 489 ment out of order because it is not germane. The question resolves upon the amendment to the motion. The amendment is to add the St. Joseph Veterinary College to the list of accredited colleges of the Committee on Intelligence and Education. Dr. Koren: It is with much reluctance that I rise to speak on this question. For days an effort has been made to prevent just this situation, and just before entering this room with this committee some effort was made whereby a solution might be had without a rehashing of everything that has transpired. The reason for this effort was the benefit of the Association, the prevention of further ill will, the accomplishment of justice toward all. The suggestion at that time was that an applica- tion be made at this time for an immediate investigation by the Committee on Intelligence and Edueation, of the St. Joseph Veterinary College, to determine whether or not it was meeting the requirements. It was also suggested that the Intelligence Committee, in order to show that it bore no ill will and no grudge and did not intend to discriminate against the St. Joseph School, request of this Association that the chairman appoint three other members of the Association to accompany that committee to make this investigation. It is not the desire, I am sure, that the Committee on Intelligence and Edueation be discredited, and I do not believe that it is the desire of this Association that the St. Joseph Veterinary School be discriminated against if it has been meeting the requirements. I regret that the motion by Dr. Conrad was not made in a little different form. I don’t know that it could be made so as to be considered germane by the chairman, but if it were possible for a motion to be made that the rules and By-Laws be suspended, and that three additional members of this Association supplement the Intelligence Committee for immediate investiga- tion, and that their findings be published in the JouRNAL, surely then the eredit should be due the St. Joseph Veterinary College and no injustice would be done; neither would the Association be on record. as supporting something that would be unfair. I make this statement since I have been mentioned by Dr. Simms (he quoted me correctly), and I think it is just that you know what has been attempted. Dr. ConNAWAY: We had a President once who said in a great erisis that it is not a theory but a condition which we confront, and the condition that confronts us now in this country is a shortage of veterinarians. You all know that I have always stood up for high standards. I would put the Cornell graduate or the State college graduate in every county in this whole country, if I could, or some of these other good schools; but we have in Missouri, where this school is located, twenty-five ecoun- ties that have no veterinarians. ‘Those people need veterinary service. I, from my college, have to teach county agents and extension men some veterinary knowledge so that they can give 490 PROCEEDINGS A. V. M. A. these people veterinary service. Not very long ago I wrote to Dr. Moore, ‘‘Have you a good man you could send to Ver- sailles, in Morgan County?’’ He wrote back that he didn’t have him. If I had written to Iowa, Cornell, Pennsylvania, or any other place, I am sure I couldn’t have gotten him there. I am not in favor of this body here going over a regularly established committee and voting this school in, but I believe it is justice to this school that we should provide some means by which they can establish their qualifications to the satisfaction of this Association that they are meeting these requirements. JT don’t believe that we ought to let them in one minute sooner than they meet the full requirements of this Association, and I believe they ought to come in through the regular channels, not through any sympathy that we may arouse here on account of injustice or misunderstanding. Therefore I renew the motion that the committee be instructed to inspect this school at the very earliest moment possible, and that they be empowered by this body to admit it back into regu- jar standing, or reject, as may be the ease. If this body here can vote that school in, it certainly has the power to give that same power to that committee. If we don’t stand by our committees we had better not have the committees. That is my stand on this matter. I hope to see this school reinstated; I hope to see it come up to the full requirement of this Association. If we had the means at our universities to do this work, we would do it; but you know that it takes a lot of money to run a great institution hke a university. It has a large number of departments. We need a large amount of money for running what we have, and if we can have a little help for running institutions and those located here and there over the country, we would be glad to have some. Send us good veterinarians. We need them. We have a good field for them. (Applause.) Dr. Corron: I wish to second Dr. Connaway’s amendment. Provided you don’t consider that germane, I want to request Dr. Moore to withdraw his motion. PRESIDENT WHITE: Dr. Moore, do you wish to withdraw your motion, your amendment, with the consent of the second ? Dr. R. C. Moore: We are now within a very few days of the opening of the school, and the only advantage that this school could gain this year by recognition would have to come within that space of time. I can see no advantage to us in any way for inspection to be made, because the time will be past to visit us with any benefit this year. If we wait another year we might as well wait regular order. Therefore I would not like to with- draw the motion. PRESIDENT WHITE: The Chair will rule the motion of Dr. Con- naway out of order. SECRETARY Mayo: It doesn’t seem to me that this Association PROCEEDINGS A. V. M. A. 49] would like to ask Dr. Moore a question. I confess I don’t see how the committee can inspect the school When it isn’t running. Sup- pose, Dr. Moore, as soon as you get fairly started you asked the Committee on Intelligence and Edueation to visit your school, and if they find on inspection that you are meeting the require- ments of the A. V. M. A., if the committee would report that find- ing to the JouRNAL of the Association, and it should be published, would that meet your needs? It wouldn’t be official action by this body, because that could not take place until the annual meeting; but I believe the Association would approve the re- port of the Committee on Intelligence and Edueation. That is only my personal opinion. You have already advertised your school, preparing to open the next session, and I confess I don’t see how recognizing that school now will be of any benefit . to you at all; but I believe it would be a source of satisfaction to your students to know that the school had been inspected after it is running, and that the report of the committee was favorable. Of course, that would still be subject to the action of the Asso- ciation, and then the students would know that this school will be recognized. It seems to me that that is the only solution of this problem as it is presented to us today. Dr. R. C. Moore: Dr. Mayo, just as I stated a few minutes ago, it would be too late to be of value to us. A few men are waiting anxiously to know whether they will matriculate with us or not. They want to, but want to,know whether they are to be recognized. This would be too late to be of value to them, and the period of matriculation will have gone by. It would be of no benefit to us whatever. If it has to come to that, we might just as well wait until the next vear, and take the regular in- specion. I can’t see where it would benefit us at all. (The amendment offered by Dr. Moore, that the St. Joseph Veterinary College be added to the accredited list of the Asso- ciation, was put to a vote and was lost.) PRESIDENT. WHITE: The question now comes on the original motion to adopt the report of the Committee on Intelligence and Edueation. (The motion was carried. ) Dr. BERGMAN: This committee has another resolution that it would like to present. ; (Dr. Bergman read the following resolution :) | can do otherwise than follow the Constitution and By-Laws. | . ery Supplementary Report The Committee on Intelligence and Education desires to submit, with its approval, the following: ‘ We, the undersigned, hereby recommend the Honorable J. M. Whittle- sey, Commissioner of Domestic Animals of the State of Connecticut, for election to honorary membership-in the A. V. M. A. Mr. Whittlesey from the time of his appointment has recognized the necessity and the advantages of cooperating with the veterinarians in his field of endeavor. In his official capacity he has at all times given =~ oe 492 Procerepincs A. V. M. A. hearty and full support to the veterinary profession of his State and nation. Soon after his inauguration into office he appointed a vet- erinarian as Deputy Commissioner. The splendid progress in the control and eradication of infectious diseases in Connecticut is largely due to his sympathetic efforts and to the hearty support he always tenders to the veterinarians. J. R. MOHLER, T. E. MUNCE, ADOLPH EICHHORN. Dr. KiERNAN: I take pleasure in moving that the report of the committee be accepted. (The motion was seconded and carried. ) COMMISSIONER J. M. WuirrLEsry: Mr. President, I rise very humbly and unworthily to thank this great Association for the honor conferred upon me, and I shall guard that honor very loyally. I wish to reiterate that there may be no misunderstand- ing, that I am unworthy of this honor that has come to me unsought and as a great surprise. You have, in adding my name to your list of honorary members, honored Dr. Charles L. Colton, a member of your Association, for his four faithful years in the service of the State as Deputy Commissioner on Domestic Animals. You have honored the present deputy, Dr. George E. Corwin, also a member of the American Veterinary Medical Association. You are honoring that good, efficient association, the Connecticut Veterinary Medical Association, who have rallied around the Commissioner’s office as their own depart- ment, and who have, with the cooperation and assistance of the Bureau of Animal Industry, with the assistance of Dr. R. L. Smith, inspector in charge, accomplished a wonderfully good work in Connecticut in the past four years. These accomplish- ments have been recognized by the Public Health Service of the State and the boards of health of the various cities, and have assisted in obtaining the confidence of the Legislature of the State of Connecticut. I do thank you from the bottom of my heart. (Applause.) Report oF EXECUTIVE BOARD SECRETARY Mayo: If it is permissible, I would like to go back to the report of the Executive Board. I have one short thing to report. The Executive Committee recommends that a Com- mittee on Membership be appointed, this committee to consist of the incoming President, the Editor of the JouRNAL, the Sec- retary, and the Chairman of the Executive Board. (It was voted, on motion, duly seconded, that the reeommenda- tion of the Executive Board be adopted.) SECRETARY Mayo: There is one item of unfinished business that I want to get finished. At the last meeting of the Associa- tion a change of Article 12, Section 1, was proposed, changing the name of the Seetion on Veterinary Colleges and Examin- erry. aN: ee Y Procerepinecs A. V. M. A. 493 ing Boards to Section on Education and Research. I move that that change in the Constitution and By-Laws be made. (The motion was seconded by Dr. Hoskins and earried.) PRESENTATION OF PAPERS PRESIDENT WHITE: We have for this afternoon two papers to be presented. I will call for the paper on “The Need of Cooperation Between the United States and Mexico for the Con- trol of Epizooties,’’ by Dr. Luis Santa Maria of Mexico. Dr. Santa Maria has requested, inasmuch as he does not speak Eng- lish fluently, that Dr. C. E. Troy of New Mexico read the paper for him. (Dr. Bemis took the chair at this point.) (Dr. Troy read the paper of Dr. Santa Maria. It was pub- lished in the JouRNAL for December, 1921, page 306.) CHAIRMAN Bemis: Gentlemen, you have heard this interesting paper. I am sure you all realize the importance of cooperation between these two countries in the matter of disease control. Do you wish to discuss this paper? Dr. EICHHORN: The paper presented a great deal of interest- ing material. Inasmuch as a colleague from Mexico has pre- sented for the first time a paper before this Association, I would suggest that Dr. Santa Maria be taken to the platform and introduced to the audience. (Applause.) (Dr. Santa Maria was escorted to the platform.) Dr. Santa Maria: I want to thank you for your welcome. On account of not being able to speak English fluently to ex- press my sentiments to you, I asked Dr. Troy to deliver the address I prepared. (Applause.) Dr. Exiason: I think it would be too bad to let this oppor- tunity go by without an appreciation of the paper just rendered by our friend from Mexico. As a representative of one of the States that has sent our sister republic a considerable number of cattle, I wish to give him the assurance that we are doing everything we can, and will render him every assistance in our power to do. those things which he wishes to have done. (Ap- plause. ) CHAIRMAN Bemis: We have one other paper on the program in the general session this afternoon, ‘‘The Surgical Treatment of Laryngoplegia in the Horse,’’ by Dr. J. W. Adams. (Ap- plause. ) j (Dr. Adams read his paper. It was published in the JourNAL for November, 1921, page 144.) (President White resumed the Chair.) PLACE OF Next MEETING SECRETARY Mayo: I have invitations to hold the next meeting in New York City, Atlantic City, San Francisco, Cincinnati, and also an invitation that will be presented from St. Louis, and 494 PrRocEEDINGS A. VY. M. A. there is an invitation from Madison, Wisconsin, which you have heard. Dr. McKenna: May I ask from whom the invitation from San Francisco comes? SECRETARY Mayo: From the Convention Board of San Fran- C1SCO. Dr. McKenna: I might say that the veterinarians of Califor- nia do not believe the meeting should come west at this time. SECRETARY Mayo: All of these invitations have come from chambers of commerce. Dr. JENSEN: In view of the statement made by Dr. Mayo that all these invitations have originated from chambers of com- merce, I desire to say that, several weeks ago it was my pleas- ure to visit the meeting of Wisconsin veterinarians. While this meeting was in session the question of inviting this Association to Wisconsin for the next year was taken up. In view of the fact that this is one of the largest dairy States of the Union, that they have never had the A. V. M. A., and there is a move- ment to have this meeting come there, I think they are entitled to have the meeting. Madison is a pretty good town; next to St. Joseph it is all right. In view of the further fact that this meeting constitutes for most of us our annual vacation, we should also select a place where we can vacate pleasantly for a little while. I don’t know where you could find more beautiful forests, or more beautiful lakes, than you can find in the State of Wiseonsin— and there is water in them, too. (Laughter.) There is a bath with every room. All you have to do is put on your bathing suit and step out. Furthermore, the University of Wisconsin is located there. It is one of the greatest institutions of learning in .this country. It has offered this Association its buildings to use while we are there, and the Animal Husbandry Department has offered its buildings for clinics, something that has been lost track of in the last two years. We have confined ourselves to papezs and dis- cussions, and a great many men are still interested in surgery, while perhaps it doesn’t seem to be called for as much as it was in years gone by. . There is a question of good fellowship involved. Almost every State in the Union has had this meeting. Although this . Association has been in existence fifty-eight years, it has never gone to Wisconsin. They ask you to come there next year. SECRETARY Mavo: In connection with this invitation, I should present the following resolution that was adopted by the Illinois Veterinary Association : WHEREAS, St. Louis is centrally located in the Mississippi Valley, reached by twenty-six different railroads and fully equipped to ac- commodate and entertain a large body of visitors, PrRocEEpINGsS A. V. M. A. 495 Be It Resolved, By the Illinois State Veterinary Medical Association, in regular session assembled, that we endorse St. Louis as the meet- ne place of the American Veterinary Medical Association in 1922, an Be It Further Resolved, That we, as an association and individually, use our influence to accomplish this end, and Be It Still Further Resolved, That a copy of this resolution be spread upon the minutes of the association and a copy forwarded to the National Secretary of the A. V. M. A., and by him brought before the proper authorities at the Denver meeting. Mr. Hatriretp (St. Louis): May I have the courtesy of the floor for a few minutes to speak for St. Louis? In addition to the resolution passed by Illinois (I apologize for not being here Tuesday) I want to call your attention to a few things. Everything that Madison has said is right. There are some things which no city can give—which I expect to prove to you—as well as St. Louis. I want to call your attention to that so I may have the foundation laid properly. I am going to present to the Secretary the formal invitation. Here is one from the St. Louis Publicity Convention Bureau. With the exception of Detroit, which has a fine bureau with a manager, the Convention Bureau of St. Louis is the only one of its kind in the world. We were kicked out of the Chamber of Commerce because it was taking 25 per cent of the income to run us. A group of men connected with the Chamber of Commerce organized this bureau, and I took hold of it and have built it up until we have an income of $750,000 and sixteen people on the staff. My publicity man is Capt. Tourron, who made him- self famous because of his success in breaking the record of recruiting U. S. Marines. I am paying that boy $4,000 a year to get publicity. I have an expert man who does nothing but arrange details; he is a high-priced man. Here I have the invitation of Governor Hyde of Missouri, in which he invites you to come to the State of Missouri. I have an invitation from the Mayor of the city, Mayor Kiel, setting forth its central location and accessibility. I have a letter here from the Chamber of Commerce, the Associated Re- tailers, and the Advertising Club, guaranteeing publicity. Those are formal invitations. I have some telegrams here which I will read. One is from Dr. Jenneman, another from Dr. Hug- gins, who is the veterinarian for the St. Louis National Stock Yards. (Mr. Hatfield read the telegrams. ) I see here a group of men that ought to be ten times as big as it is. When you go to extreme points in the country a great many can not attend. Denver has wonderful weather, wonderful hospitality, they know how to entertain, and they have the mountains. We can’t move the mountains to St. Louis. I am not making any invidious comparisons, but if you watch the 496 ProcEEepiIncs A. V: M. A. thermometer you will find that the thermometer of St. Louis has run from two to three degrees less than Chicago and other cities around there. It costs money in these times to travel. With the railroad rates up you want to get a cen- tral point which does not take so much money. We have had 369 conventions whose attendance we have increased from 25 to 30 per cent because they selected a central city. The one thing I want to emphasize is our ability to take eare of you. Pardon the personal reference, but I am President of the National Association of Convention Bureaus. I know what other cities are contending with in regard to conventions; they try to pattern as much as they can after our service. It is one thing to invite a convention te a city and quite another to have them all go away feeling that was the best convention they ever had. Dr. Howard wrote me a letter and made the statement that they did not expect to have such service and such publicity anywhere until they came back to St. Louis in sixteen years. It was with his letter that I was successful in landing the Ameri- can Bar Association. Speaking of conventions, you know how careful the A. M. A. is. After four years I finally landed them. They are going to meet in St. Louis in the spring, in the last week in May. We are taking care of conventions by giving a service that is twofold, first to build the attendance. I will spend any amount of money that your national officers will tell me is jus- tified to build this attendance and get you new blood. I have met some of the fellows around here who are professional. I don’t mean to criticize them. They are here representing other than what I would call the practitioners. It is all Greek to me, yet I hear a wonderful paper read here with less than a hun- dred people sitting here to listen to it. It is spread out through the technical journal. To go back to the building up of attendance, we lay out a campaign after your formal notice has gone out to your mem- bers announcing where you have decided to hold your meeting, and we start in, subject to the approval of every word, so it will be ethical according: to your standards. The Governor writes a letter on his stationery, we pay the postage, and it goes out of our office. We work that so that we may havé series of a half dozen. We send those letters out to members and men who have not been to conventions for several years. The man zets a letter from the Governor of Missouri; that attracts his attention. A little later on he gets a letter from the Mayor, then from the Chamber of Commerce, then a local society, per- haps the stock yards. The psychology is really this: ‘‘ Those fellows really want me! I am going to go!’’ Our publicity man will prepare cuts and material to go into your JOURNAL Proceepines A. V. M. A. 497 and other official publications several months before the conven- tion, telling all about it. You are not going to be overcharged in St. Louis. The hotels are the Statler, Jefferson, Planters, Marquette, etc. We whipped the hotels into line, and if one of them goes back on its promise not to advance rates during conventions, they don’t get any more conventions until they make good. (Mr. Hatfield read telegrams from the Hotels Planters, Jef- ferson, Claridge and Marquette. ) I want to emphasize that we have the service, we will give the publicity. Our publicity is ethical. Suppose we had your program a month or six weeks in advance, and saw that Dr. Smith of Atlanta, for instance, was going to read a paper. We would try to get from Dr. Smith a paragraph or two on what he was going to say. If you paid for all this publicity it would cost you a million dollars, a dollar a line, which is cheap pub- licity. Here you are, a constructive organization with wonder- ful ideas to sell; you want new members, new blood; you want to sell the idea of your organization to the public. We arrange a press room, and Capt. Tourron secures the papers from you, as far in advance as you can furnish them, and commences to put them through. You will censor it, of course. We will see that anything you release gets into the papers. The manager of every daily paper is a member of this bureau. I do want to say a word about the ladies. You haven’t enough ladies here. We have in St. Louis an organization headed by a lady who is the hospitality director and meets with local ladies and arranges the program of entertainment. If you bring the ladies you won’t have to’worry ; your wives will be taken care of. ( Applause. ) PRESIDENT WHITE: You have heard the invitation from the city of St. Louis. Are there any more representatives of any other cities who would like to be heard? Dr. JENSEN: We are invited to go to St. Louis. We haven’t had a single veterinarian from St. Louis at a convention in some time. Mr. Hatfield has come directly from headquarters to invite you, and he spent money in coming. It would be a good thing to go there and stir up those veterinarians. Dr. Exvitason: I am afraid that it is useless to talk to this audience after it has been subjected to that much oratory. We don’t come here inviting you to come to Madison entirely for selfish motives. We believe that we ean do something for the organization. If I didn’t honestly believe that I wouldn’t ask you to come. I can’t boast of the terribly extravagant ac- commodations that we can give you; I can’t boast of a good many elaborate times we can give you; but I guarantee you this, if you come there: You will all have to work and get into the meeting, and feel, when you go back, that you have been to a real veterinary convention. 498 PrRocEEpINGS A. V. M. A. We assure you that if you come to Madison you will be taken care of and housed, and it will be at rates that any one can afford to live on. There will be no seven or eight-dollar-a-day hotels, but we will take care of you and we will give you ac- commodation that will amply house you and keep you comfort- able while you are there. Dr. Connaway: In talking these matters over with Dr. Jen- sen at the beginning of this meeting I felt somewhat as he did; but the presentation which Mr. Hatfield has made convinces me that for the best interest of our Association we ought to go to some place where we can get the very largest attendance and have that cooperation of a publicity man who will make it a success. With all the promises which Mr. Hatfield has made, and with his genius for getting crowds to St. Louis, I believe that we would have in that city next year one of the largest at- tendances we have ever had. Moreover, it would be close to where Dr. Kinsley would be, so he could cooperate, and where every other veterinarian that should have been here can be drafted into the service and made to work harder than any veterinarian ever worked before. We need more members. Dr. Craic: I move that the Association recommend to the Executive Board that we meet in Madison, Wisconsin. (The motion was seconded by Dr. Bergman.) Dr. LockHartr: Strike out the word ‘‘Madison’’ and substi- tute ‘‘St. Louis.’? I move to amend to that effect. (The amendment was seconded. ) Dr. ConNaway: I would move as a substitute that the house be divided and those in favor of Madison gather on one side and those in favor of St. Louis gather on the other side. PRESIDENT WHITE: That is a good suggestion. Secretary Mayo: I think that matter ought to be referred to the Executive Board with an expression of opinion. I am not a member of the Executive Board, but I am sure the Executive Board would take every factor into consideration and give it the most careful thought. They represent the country as a whole. Dr. STANGE: The most representative committee we have is elected by postal ecard ballot for a period of five years, and it seems to me that if we are going to follow the policy of letting small minority groups decide these questions we are going to have a hard time doing for the Association what we want to do. Dr. JAKEMAN: Is not this motion merely a recommendation to the Executive Board? Is it not in their hands finally to decide? PRESIDENT WHITE: The Chair so understands. Dr. JENSEN: I don’t believe I got that understanding from the minutes yesterday. Has it come to pass that this entire organization is destined to be handled by five men? Is the entire membership that is paying dues entitled to know what shall happen? JI declare to you that executive committees are human and they make mistakes. They ought not to take the oo _ plain Proceepines A. V. M. A. 499 privilege of the membership out of their hands and put it into the hands of a committee of five men. I have had some experience with the Executive Board. I had to go on the floor to fight a bunch, and I want to tell you I beat them hands down. I would not for one minute submit to the final vote of the Execu- tive Board on the matter. Dr. Connaway: In making my motion it was my under- standing that the settlement of this should ultimately go to the Executive Board. (Dr. Connaway’s substitute motion was seconded and carried, and a standing vote was taken on each city. The result as an- nounced by Secretary Mayo was 40 in favor of St. Louis and 46 in favor of Madison.) SecreTaRY Mayo: As I understand this, it is referred to the Executive Board as the expression of opinion, 46 being in favor of Madison and 40 in favor of St. Louis. Dr. JENSEN: May I ask the Secretary to read for the assembled people, as a matter of courtesy to them, the exact wording of that matter in the By-Laws? (Secretary Mayo read the section in the By-Laws. ) Dr. ConNAWAY: My intention was to get the expression of this small body. There are so few of us here to represent such a large body and to say where we should go, that my intention was that the ultimate settlement should be left with the Execu- tive Board. Things may come up between now and the next meeting that would place the meeting in some other location altogether. What we want to work for is not for local interests, not for the boosting of Missouri or Wisconsin, but to do those things which will be tor the best interests of our Association. We want to do those things that are going to increase our mem- bership. The Secretary has called attention to that time and time again. That is what we need. We should go where we will derive the most benefit. If we can get it by going to Madi- son, by all means let us go there. If we can get it by going to St. Louis, let us go there. I think some central location where there is some big booster like this one who will go after every section of the country may be of help to us. Maybe Mr. Hat- field will help us even if it goes to Wisconsin. Mr. Harrrevp: Surely. Dr. JENSEN: Let us not hedge the issue. I should think that in all fairness we should consider only our own best interests. I want to be fair, even though you may think I am knocking my own State. Dr. Stuns: At a recent joint meeting of the British Columbia Veterinary Association and the Oregon State Veterinary Asso- ciation, a resolution was unanimously passed inviting the A. V. M. A. to meet in Portland, Oregon, in 1925. In that year there will be at Portland an international exposition, so we can get very favorable railroad rates. We feel we are due some con- 500 ProcEEepiInGs A. V. M. A. sideration from the Association. We have a good many mem- bers of the A. V. M. A. out in the Far West country, and the Association has never met in that section. Somebody will men- tion San Francisco, but the people north of that, miles and miles away, do not feel that you are meeting in their vicinity. Get up to British Columbia. We have good loyal members in those Northwest States, members who pay their dues and boost the Association. We have never had a meeting anywhere in our vicinity, and we are extending at this time an invitation coming not from the commercial clubs, not from the hotels, but from the veterinarians of the Northwest, who hope you may be able to get up there in 1925. (Applause.) Adjournment. FRIDAY MORNING, SEPTEMBER 9, 1921 GENERAL SESSION The meeting convened at 10:30 a. m., President David S. White presiding. Report oF EXECUTIVE BOARD PRESIDENT WHITE: We will now have the report of the Execu- tive Board. SecreTARY Mayo: Mr. President, we have one more applica- tion that came in last night of Alva C. Stein, graduate of the Ohio State University, College of Veterinary Medicine, 1911, properly vouched for and approved by the Board. (Upon motion of Dr. Sisson, duly seconded, the application was accepted. ) REPORT OF COMMITTEE ON EMBLEM PRESIDENT WHITE: There are a few committees yet to report. J will call for the report of the Emblem Committee. Dr. McKenna: Dr. Bennett has asked me to report. (Dr. McKenna read the report of the Emblem Committee, as follows :) Your committee has gone over the various drawings and sugges- tions which have been submitted to it, and recommends for adop- tion the emblem which has been adopted by the California State Veterinary Medical Association. The design for this emblem was drawn by Dr. H. B. Wintringham of Fresno, Calif., and is the caduceus with a superimposed V as used for collar ornament in the Veterinary Corps of the Army. This is placed in a maroon disc surrounded by a light band. The drawing as we are showing you here today is the exact size. This plate will have a metal border, inside of this a circle of white, hard enamel, and the center of the plate will be hard enameled in red with medical emblem and letter V showing in metal. The plates will have a 5-inch lug soldered on the back, which is long enough to extend clear through the radiator, and we will also furnish an iron washer and a wing nut for attach- ing. The metal parts of this plate will be finished in either brass or nickel plate, whichever is preferred. ae * ProcrEepiIncs A. V. M. A. 501 One of the principal values these emblems will have will be for use on a member’s automobile. Restrictive automobile legislation is constantly being enacted, and it is believed that our profession will be given the same recognition in regard to such legislation as time limit for parking automobiles in congested districts as is given mem- bers of the American Medical Association when their emblem is on a car. The California State Association has taken the matter up with Bastian Brothers of Rochester, N. Y., the makers of the American Medical Association emblem, and has received the following quota- tion: Lots of 250 emblems, $2.30 each; 350 emblems, $1.90 each; 500 emblems, $1.60 each. No doubt in larger quantities a better price could be obtained. S. E. BENNETT, Chairman; JOHN F. MCKENNA, Committee on Emblem. (A motion was made by Dr. Mayer, seconded by Dr. Fox, that the report be accepted. ) SECRETARY Mayo: I would like some information as to how this emblem is to be supplied. Is the Secretary to order them and supply them to the members ? * on a ASP 2y Emblem Adopted by American Veterinary Medical Association 502 Procrepines A. V. M. A. Dr. McKENNA: My idea in regard to this is that if the report of the committee is adopted a motion should be made on the floor in reference to how they shall be distributed. (The motion to adopt the report was carried. ) Dr. McKenna: In reference to the distribution of these em- blems from the manufacturers, the Secretary of the State Asso- ciation of California has so far had about fifty orders from the veterinarians in California. He has sent out a card, and his instructions from the State Association are that no one can secure this emblem unless he is a member of the Association. I believe that is the procedure in the A. M. A.; they are handled through the resident or the local secretaries, and unless a man is a member of the National Association he is unable to secure the emblem. I believe that rule would be a good one in this Association. It would perhaps stimulate membership in both sectional and State associations. Dr. Hoskins: I believe it would be a good idea if the report of this committee would be published in an early issue of the JouRNAL. The Committee on Emblem might draw up some sort of an additional report outlining a probable way in which these emblems could be supplied, and through the Resident State Secretaries some idea could be obtained of exactly how many might be wanted. I believe that could be done much more rapidly through the JourNAL than any other means of which I know. Secretary Mayo: I would move that this be referred to the Executive Board with recommendations from the Emblem Com- mittee. The Executive Board will meet in Chicago the latter part of November, and they have under consideration at that time various plans for increasing the membership of the Asso- ciation, and I think that the Board could very properly devise a plan for distribution, with the suggestions from the com- mittee. (The motion was seconded by Dr. Fox and carried.) Report oF BupGET COMMITTEE PRESIDENT WHITE: I will now call for the report of the Budget Committee. (Secretary Mayo read the report of the Budget Committee, as follows:) Presidents AH). .3 oo Pe oe eer ace Te eee $500.00 Intellicencesandwhducaiionige eee eer ee ae eee 1,000.00 Levislation:. “tet 2.55 Otc ee er eee to eee RSD OULU Salmon Memorial Fund, limited to postage and incidentals........ 50.00 Abortion Committecee ee ee ee See 500.00 PPOASUITOR scsi SSS. ee eae rete ee ee 300.00 Pharmaey =). oe) 8 ee ae er ee rece ae ee 250.00 AnatomicalicNomenclature. 2:25.20 Se ee eee 50.00 Proceepincs A. V. M. A. 503 Oashares torse: Association of “America....-22 2... 50.00 Necessary running expenses of JOURNAL office at Washington and expenses of Secretary’s office. m D. S. WHITE, M. JACOB, N. S. MAyo, Budget Committee. Dr. Hoskxrys: I move that the report be received and ap proved. (The motion was seconded and carried.) REPORT OF COMMITTEE ON ANATOMICAL NOMENCLATURE PRESIDENT WuHiTE: I will now eall for the report of the Com- mittee on Anatomical Nomenclature. Dr. Sisson: In the absence of Dr. Murphey, the chairman, I will simply make a brief verbal statement to the effect that so far as I know no meeting of the committee has been held and consequently there is no formal report to be offered. I think it would probably be well to continue the committee, or at least a committee, in view of the fact that there is a proposition to have an International Committee on Nomenclature, and of course this body should have some representation on the National Com- mittee or the International Committee. PRESIDENT WHITE: You have heard the statement of a mem- ber of the Committee on Anatomical Nomenclature. It will require no motion. Report OF COMMITTEE ON RESOLUTIONS PRESIDENT WHITE: Is the Resolutions Committee ready to re- port? (Dr. Jungerman read the report of the Committee on Reso- lutions, as follows:) Resolution * Whereas, There has recently been a renewal of activities in the United States against animal experimentation, under the misleading name of a campaign against vivisection, And whereas, Many false statements have been made to the effect that medical men do not regard animal experimentation as of value and do not approve of it, and that such experimentations are cruel and unnecessary; Therefcre be it Resolved, That the American Veterinary Medical Association hereby places itself on record as favoring animal experi- mentation, and as expressing its belief that animal experimentation is of the highest value, that it has furnished us with a large part of our present knowledge, and that it is the only method by which we can hope to solve many of our present problems, to save our animals from suffering and humanity from economic losses among its livestock. And be it further Resolved, That we condemn and disapprove the campaign for the prevention of animal experimentation and all meas- ures to place the supervision of such investigation under persons without sound medical, veterinary or biological education and training. 1This resclution is given in the amended form as adopted. 504 PROcEEDINGS A. V. M. A. And be it further Resolved, That we condemn as false the state- ments to the effect that animal experimentation is cruel or provocative of cruelty, it being a matter gf common knowledge among veterinari- ans that animals used for experimentation purposes are handled with care and kindness, are not treated cruelly, and are given anesthetics whenever necessary to prevent suffering. Resolution Whereas, The American Veterinary Medical Association, while in convention at the city of Denver, has received the assistance and hospitality of the local committee and veterinarians of Colorado, the civic authorities and other organizations of Denver, the Albany Hotel, the Denver Cab Company, and the Parl Floral Company: Be it Resolved, That the Association hereby records its apprecia- tion of the many courtesies extended and the assistance received from each and all of them, and that the thanks of the Association be ex- tended to them. (A motion was made and seconded that the report be adopted.) Dr. Jones: The first resolution states that experimentation should only be by medical men and veterinarians. Of course under the supervision of medical men and veterinarians. Biolo- gists use a great many more animals than either of us, and, of course, physiologists need not be medical men or veterinarians. T think that should be amended. Dr. JUNGERMAN : I think that reads that supervision should be maintained by men having medical training. Dr. Maurice C. Hau: I think the suggestion is a good one. In drawing up this resolution I was writing rather hastily. It would be well to make that ‘‘of sound medical or veterinary scientific training,’’ the idea being to head off the sort of super- vision which is in vogue in England. The persons carrying on antivivisection campaigns have been sufficiently successful in England to hamper the work of animal experimentation very materially. Most people opposed to animal experimeftation are doing it on sentimental grounds, and they have a supervision in England which is very restrictive, very injurious to English scientific research. In this country we have had similar campaigns going on, and tney will be going on in the future. Unless we take steps to head those campaigns off, American investigations of scientific medication in the biologie field will be hampered in the same way. There has been recently a fight in California on this sub- ject. It has been brought up in the United States Congress a number of times, and very recently it has come up again. The veterinary profession, I believe, would be unanimous on this subject. I don’t think it would be necessary to ask the A. V. M. A. to support this resolution. I have no doubt of their sup- port, but I don’t know that all of the members appreciate how very serious the situation is and how necessary it is that we combat in every possible way this propaganda to stop these experimentations. The men who are making the campaign are Procreepincs A. V. M. A. 505 putting it first on the basis of preventing experiments on dogs, but that is only a hindrance. When they have achieved that they will have stopped the experimentation entirely if they ean. In order to expedite the consideration of this resolution, I move that the Committee on Resolutions insert the word “‘bio- logical’’ in addition to the words ‘‘medical and veterinary’’ in reterence to supervision. - (The motion was seconded by Dr. Jones.) PRESIDENT WHITE: This comes as an amendment to the report of the Committee on Resolutions by adding certain words in order to include the scope of those who indulge in this practice— namely, it would include biologists and other competent scien- tists. (The amendment was carried. ) PRESIDENT WHITE: Now the question is on the original motion as amended. (The report of the committee as amended was adopted.) REPORT OF SALMON MEMORIAL COMMITTEE PRESIDENT WHITE: We will now have the report of the Sal- mon Memorial Committee. Dr. Monuer: In the absence of Dr. Brenton, I have been re- quested to present the report: (Dr. Mohler read the report of the Salmon Memorial Com- mittee, as follows :) The report of the secretary-treasurer of the committee, the late Dr. W. Horace Hoskins, rendered at the Columbus meeting last year, showed that $3,956.40 and one $500 United States bond had been col- lected for the fund. Since the 1920 report was made the sum of $38 additional has been collected. The funds that have been collected thus far have been placed on deposit in the Rittenhouse Trust Company of Philadelphia in the name of the Salmon Memorial Fund. This deposit bears interest, but your committee is unable to report at this time the exact amount of interest that has been credited to the account during the past year. The will of the late Dr. W. Horace Hoskins, who was largely in- strumental in the creation of the memorial to Dr. Salmon, and who has been so active in the collection of subscriptions to the fund, con- tained the following bequest: “That the sum of $100 be given to the Salmon Memorial Fund as a tribute to one of the most complete lives of unselfishness and true public service ever lived by any member of my chosen profession.” Your committee recommends that the Salmon Memorial Committee of this Association be continued, that an effort be made during the coming year to collect the balances of unpaid pledges, about $5,500, and that the fund, or such part of it as may be deemed wise, be invested in Government bonds, the interest from these bonds to be -made available as early as possible for the use of some worthy young man needing financial assistance in securing a veterinary education. 506 PRocEEDINGS A. V. M. A. Receipts, Salmon Memorial Fund, 1920-21 Br. J, Debishe sh Foi Ed ee att teehee ole Ete Reoa i aes $1.00 Dri (On hOwelissec. sien she hoch cen ee. SU) ele ee eee ee 1.00 1 ey ams OFT or 0) OY RN racine ORDA Tie deses OW hee aaa Leo Menahem ce ities Seeley | 1.00 1 By id DB Papp Cees CN(a pico ee eens Noein Melitta her athe Ae Ra <1 Se ei Ee 10.00 California State Veterinary Medical Association... 25.00 ch La) 6621 a ea A Pa ies As Mlk i ea eb el re a A he $38.00 S. BRENTON, Chairman, Salmon Memorial Committee. Dr. ErcHHoRN: I move that the Association express its pro- found gratitude for the bequest of Dr. Hoskins. (The motion was seended and carried.) Dr. JAKEMAN: I move that the report of the committee be re- ceived. Ue (The motion was seconded and carried. ) PRESENTATION OF PAPERS PRESIDENT WHITE: That seems to conclude the reports of com- mittees. We have two papers, one entitled ‘‘Inspection of the Fish Food Supply of the United States Army,’’ by Major George Lytle. Major Lytle is not here. The second paper is ‘‘Mary- land Plan for the Eradication of Hog Cholera,’’ by Dr. R. C. Reed, of College Park, Md. (Dr. Reed read his paper, which will appear in a later issue of THE JOURNAL.) PRESIDENT WHITE: The next paper will be ‘‘Types of Bacillus Bovisepticus Encountered in a Dairy Herd,’’ by Dr. Fred R. Jones, of Princeton, N. J. (Dr. Jones read his paper, presenting it with illustrations on the board. The paper was published in THE JouRNAL for De- cember, 1921, page 271.) PRESENTATION OF NEW OFFICERS PRESIDENT WHITE: This concludes all of the papers. If there is no unfinished or new business, the next item will be the in- stallation of officers. I will appoint Dr. Sisson and Dr. Goss to present the President-elect, Dr. Kinsley. (Drs. Sisson and Goss conducted Dr. Kinsley to the chair.) PRESIDENT WHITE: It is a pretty little custom which has come down to us through the years to have the outgoing President present to the incoming President a gavel, and a gavel with a history. This badge of authority was presented to this Associa- tion by the late W. T. Monsurat, of Honolulu, Hawaiian Islands, at the Minneapolis Convention in 1902. I believe this late mem- ber was known as Honolulu Bill. There is an inscription on this gavel, the meaning of which I do not know, but I assume that it greets the incoming President with a wish of prosperity, the best of luck and the heartiest cooperation of the member- ProcEEepincs A. M. V. A. 507 ship of this Association. I trust that he will find his service as pleasant and as inspiring as mine has been. I hope also that he will receive your courteous treatment, hearty cooperation, respect and helpfulness that I have received. Dr. Kinsley, per- mit me to present this gavel. (Applause.) Dr. Kiysuey: Mr. Chairman, Gentlemen of the Association, I first wish to thank you cordially for the honor you have con- ferred upon me. In accepting the executive position of this Association I am. not unmindful of the obligation that I will have to meet. I am going to ask each and every one of you, as members, committeemen and officers of this Association, to assist, help and cooperate in every way in making the coming season one of the best Association years we have had. (Ap- plause. ) PRESIDENT WHITE: Permit me to install and introduce two of the five Vice-Presidents, the First and Second. I don’t know where the other three are. A member remarked to me at this meeting that we had too many Vice-Presidents. I told him there was no evidence of that fact, as none of them was present. If you desire, and if these men are anxious to deliver themselves of a short oration on this occasion, we shall be glad to hear from them. Dr. Lams: Mr. Chairman and Gentlemen, I am not going to try to inflict any address upon you, but I want to thank you for the honor conferred. I appreciate the fact that to be Presi- den of this Association is the highest honor that can be con- ferred on any veterinarian. Naturally I say that the office of Vice-President is the second highest honor, so I consider I have been elected to receive the second highest gift of the Association, and I appreciate it as such. At the same time I realize that this honor was not conferred upon me because my name hap- pened to be Lamb. It was, I think, conferred upon the vet- erinarians of this district with me as their representative. I don’t presume that the office of Vice-President will require any arduous labors on my part with such a man as President as Dr. Kinsley with his well-known ability and activity in all lines as we know him, but if occasion should require that any duty devolve upon me, I assure you I would be ready and willing to assume it to the best of my ability. (Applause.) Dr. McKenna: Mr. Chairman and Gentlemen, I appreciate the honor of being elected Vice-President of this Association, and I assure you that the veterinarians of the Far West appre- ciate recognition in this Association. I assure Dr. Kinsley and the other officers of my hearty cooperation, and I will be glad to assist In any way I can in our part of the country. (Applause. ) (A motion was made, seconded and carried, to adjourn the convention sine die.) (Proceedings of section meetings will appear in February JOURNAL. ) 5OS ASSOCIATION NEWS EXECUTIVE BOARD MEETING St. Louris GETS NEXT CONVENTION At the meeting of the Executive Board of the A. V. M. A,; held in Chicago on November 27, the Board voted that the 1922 meeting of the A. V. M. A. would be held in St. Louis, Missouri, and that the dates would be August 28 to September 1, inclusive. In connection with the program for the next meeting the Board thought it advisable to have a two days’ clinic to be in charge of the best operators that could be procured, the clinic to be devoted to diseases of cattle, hogs, dogs and poultry, to- gether with general surgical subjects. The Executive Board authorized President Kinsley to ap- point a committee of three to cooperate with the National Formula Committee with reference to new and non-official vet- erinary remedies. President Kinsley appointed Drs. Brumley of Columbus, Klein of Philadelphia, and Milks of Ithaca, New York, the first named being chairman. The question of the legality of the election of officers for the Sections on ‘‘Sanitary Science and Police’’ and ‘‘ Education and Research’’ at the Denver meeting, was brought before the Board. The Board ruled that the officers of the section on “‘ Education and Research’’ for the past year, should continue for the present year. The Secretary was authorized to purchase 500 automobile emblems, and that these emblems were to be sold to members for $1.25 each. Treasurer Jacob made a report on the funds of the Associa- tion. The Board authorized Treasurer Jacob to purchase $10,000 worth of American or Canadian Government bonds. N. S. Mayo. IT WILL PAY The Committee on Increasing Membership of the A. V. M. A., consisting of President Kinsley, Dr. Hilton, chairman of the Executive Board, Dr. Mohler, editor of the JoURNAL,*and Seec- retary Mayo, held a meeting recently in Chicago. It is planned to put on an active campaign to increase the membership, and it was decided that every member of the Asso- ASSOCIATION NEWS 509 ciation who is not an official will receive a rebate of $1.00 on his annual dues for every application for membership that he sends in. Five new applications. will pay the member’s dues for a year. If he gets more than five the amount will be applied on the next year’s dues. Application blanks will be sent to any member who desires them, if he will drop a postal card to Secretary Mayo. AN HONOR FOR THE A. V. M. A. The National Veterinary Society of Cuba, through its Presi- dent, Dr. B. Crespo, has cabled an invitation to the A. V. M. A. to send a delegate to the Fifth Annual Medical Convention of Cuba. The delegate is to be the honored guest of the Cuban National Veterinary Association, who will pay all expenses of the trip to Cuba as well as all expenses during the week of the convention in Havana. In response to this hospitable and generous invitation, Presi- dent Kinsley has named Dr. R. P. Marsteller, of College Station, Texas, to represent the A. V. M. A. at this convention. The Medical Convention of Cuba is composed of physicians, veterinarians, dentists and pharmacists. A splendid scientific and practical program has been arranged and we know that all members of the American Veterinary Medical Association will be much interested in the report that Dr. Marsteller will make of this splendid convention. We feel sure that the A. V. M. A. will express its appreciation for the honor conferred by the National Veterinary Association of Cuba. N. S. Mayo. NOMINEES FOR EXECUTIVE BOARD The following are the nominees selected by postal card vote from among whom a member of the Executive Board will be elected to represent District No. 6: J. H. Bux, Arkansas. © B. W. Conrad, Kansas. D. F. Fox, California. H. Jensen, Missouri. N. F. Williams, Texas. 510 ASSOCIATION NEWS A. V. M. A. COMMITTEE APPOINTMENTS President Kinsley has appointed Dr. James Robertson, of Chi- cago, a member of the A. V. M. A. Committee on History, to succeed Dr. R. C. Moore, who resigned. President Kinsley has appointed Dr. J. P. Turner, 918 O street northwest, Washington, D. C., and Dr. R. R. Clark, New- port News, Va., a committee of the A. V. M. A. to cooperate with similar committees representing medicine, dentistry and pharmacy, in the preparation of a model Federal Narcotic Law or a revision of the present law. The committee will gladly re- ceive any suggestion from the profession regarding this matter. UNITED STATES LIVE STOCK SANITARY ASSOCIATION The twenty-fifth annual meeting of the United States Live Stock Sanitary Association was held at the La Salle Hotel, Chicago, Illinois, November 28, 29 and 30. This annual event is growing in interest and importance each year. It is esti- mated that notwithstanding the fact that it was International Livestock Exposition week and the meetings of the National Association of Commissioners, Secretaries and Departments of Agriculture, and the National Association of Marketing Bureaus were scheduled to take place at the same time, and that the Illinois Veterinary Medical Association also was to meet in Chicago on December 1 and 2, there were in attendance over 400 veterinarians, U. S. Bureau of Animal Industry officials, State regulatory authorities, livestock breeders and others. An ex- cellent program was prepared for the occasion and it was notable that with two exceptions all whose names appeared on the pro- gram responded when they were called. President W. F. Crewe ealled the meeting to order and re- quested Mr. H. R. Smith, Livestock Commissioner of the Chicago Livestock Exchange, to deliver the address of welcome in the absence of Dr. John Dill Robertson, Commissioner of Health, who was expected to welcome the members of the Association to Chicago on that occasion. Commissioner Smith ably per- formed this unexpected assignment, and Hon. J. H. Mercer, Livestock Commissioner of Kansas, responded in the pleasing manner for which he is noted. In a very earnest and timely ad- dress, President Crewe referred to the establishment of the as- Association NEWS 511 sociation, its development, its progress, and some of the prob- lems that now confront it and the livestock industry. During the remainder of the first session, the report of the Committee on Legislation was presented by H. R. Smith, chairman, and a period was devoted to a memorial for the only departed mem- ber, Dean W. Horace Hoskins; Dr. J. I. Gibson, of Blooming- ton, Ill., led in the memorial exercises. Dr. J. F. DeVine and Major Charles Jewell delivered appropriate eulogies on the life and work of Dr. Hoskins, who will be remembered as a man among men, as a successful practitioner and educator, and for his zealous, untiring efforts to advance the veterinary profession to a higher plane of usefulness, respect and recognition. The after- noon of the first day was devoted to a joint session of the U. S. Live Stock Sanitary Association, the National Association of Commissioners, Secretaries and Departments of Agriculture, and the National Association of Marketing Bureaus, with Hon. Alva Agee, Secretary of the New Jersey Department of Agriculture, in the chair. At this session the association was honored by an address from Hon. Henry C. Wallace, Secretary of Agriculture. Immediately following the address of the Secretary, Hon. Fred Rasmussen, Secretary of the Pennsylvania Department of Agri- culture, Mr. A. J. Glover, editor of Hoard’s Dairyman, and D. V. Moore of Iowa, addressed the assemblage on the ‘‘ Value and Desirability of a Joint Session Yearly of the U. S. Live Stock Sanitary Association with Other Bodies Represented. ”’ Dr. O. H. Eliason, State Veterinarian of Wisconsin, read a paper on the subject of ‘‘Live Stock Regulations at Fairs and Expositions, with Special Reference to Accredited Herds,’’ and . Dr. J. A. Kiernan, Chief of the Tuberculosis Eradication Di- vision, B. A. I., expressed his views on ‘‘ What Commissioners of Agriculture Can Do to Assist the Tuberculosis Eradication Movement.’’ The discussion of these papers was ably led by Hon. D. M. Davidson Director of Agriculture, Springfield, Ilh- nois; Hon. H. H. Halladay, Commissioner of Agriculture, Lan- sing, Michigan; Hon. C. P. Norgord, Commissioner of Agri- culture, Madison, Wisconsin; and Hon. E. C. Brigham, Commis- sioner of Agriculture, St. Albans, Vermont. It was a mutual pleasure and benefit for the members of these closely allied as- sociations to meet in joint session. This joint meeting afforded an opportunity to become personally acquainted and more fa- miliar with the relation of these organizations to each other and Fi ee Association NEws the work each is doing on problems in which all are interested. The trend of the discussion plainly indicated a consensus of opinion that as these organizations hold their annual meetings about the same time, arrangements should be made for a joint session each year. The first session on the second day was devoted to abortion disease among animals. Dr. W. L. Williams, Emeritus Professor of Veterinary Medicine, Cornell University, Ithaca, New York, read a paper on ‘‘Suggestions for the Improvement of the Re- productive Efficiency of Cattle.’? Dr. E. C. Schroeder, Superin- tendent of the B. A. I. Experiment Station, Washington, D. C., read a paper on ‘‘ Bureau of Animal Industry Investigations on Bovine Infectious Abortion,’’ and Dr. T. H. Ferguson, a vet- erinary practitioner of Lake Geneva, Wisconsin, who has given the subject much study, read a paper on ‘‘Herd Control of In- fectious Abortion in Cattle.’ The papers read by Dr. Williams and Dr. Schroeder were among the most prominent features of the meeting, both on account of the recognized standing of the authors in the field of research and the scientific and economic importance of their subjects. The discussion that followed the reading of the papers commanded more than ordinary attention, owing to the participation by men prominent in their special hnes, among whom were Dr. C. P. Fitch, Chief of the Division of Veterinary Medicine, University of Minnesota; Dr. Adolph Eichhorn, Director of the Veterinary Department of the Lederle Antitoxin Laboratories, Pearl River, New York; Dr. Ward Gilt- ner, Professor of Bacteriology and Hygiene, Michigan Agricul- tural College; Dr. Edward A. Cahill, Director of the Pitman- Moore Laboratories, Indianapolis, Indiana; Dr. George Dick, Professor of Animal Industry at the Veterinary Department, University of Pennsylvania; Dr. J. W. Connaway, Professor of Veterinary and Comparative Medicine, University of Missouri; Dr. C. E. Cotton, State Veterinarian of Minnesota; Dr. J. G. Ferneyhough, State Veterinarian of Virginia; and Dr. C. W. Eddy, Veterinarian for Tellings-Belle Vernon Company, Cleve- land, Ohio. At the afternoon session on the same day, Dr. Eichhorn spoke on ‘‘Control of Anthrax by Simultaneous Treatment’’; Dr. W. J. Butler, State Veterinarian of Montana, discussed ‘‘ Methods of Eradicating Scabies in Sheep’’; and Dr. James S. Healy, the B. A. I. representative in cooperative tuberculosis eradication ede 7 OO ASSOCIATION NEWS 51 work in Wisconsin, addressed the association on the ‘*‘ Methods by Which Tuberculosis is Spread’’ among animals. The forenoon of the last day of the meeting, November 30, was devoted to the consideration of swine diseases. Dr. C. H. Stange, Dean of the lowa State Veterinary College, presented the Report of the Committee on Infectious Swine Diseases; Dr. U. G. Houck, Chief of the Division of Hog Cholera Control, B. A. I., Washing- ton, D. C., presented the Report of the Committee on Interstate and Intrastate Shipment of Swine, and in the absence of Dr. T. P. White, Dr. W. T. Spencer, Livestock Commissioner, Omaha Livestock Exchange, presented the Report of the Committee on Hog Cholera Control. Dr. M. Dorset, Chief of the Biochemie Division of the Bureau of Animal Industry, gave a very inter- esting and instructive talk on swine diseases in which he in- formed the association of the results of his experiments with suipestifer bacterins. Dr. Edward A. Cahill read an interesting paper on *‘ Factors Influencing the Control of Swine Diseases,’’ which was discussed by Dr. George E. Corwin, Commissioner on Domestic Animals, Hartford, Connecticut, and Dr. B. F. Edgington, State Veterinarian of Ohio. Dr. A. T. Kinsley, Deputy State Veterinarian of Missouri, and Dr. W. W. Dimock of the Department of Veterinary Science, Kentucky Agricultural Experiment Station, led in the discussion of Dr. Dorset’s ex- periments. The reports of the Committee on Swine Diseases re- sulted in the most spirited discussion that oceurred during the meeting. Dr. Kinsley’s criticisms of the Report of the Commit- tee on Infectious Swine Diseases were met by prompt, vigorous retorts from the members of the committee. Both the criticisms and the replies were appreciated, especially by the members who were interested in the differential diagnosis and treatment of swine diseases. The last session of the meeting was devoted to the reports of committees and the election of officers. The Committee on Reso- lutions presented three resolutions, in effect as follows: That, in the production of milk, whether for consumption in cities or in rural homes, the tuberculin testing of cattle should be by all means insisted upon as an indispensable measure of pre- venting the dissemination of tuberculosis. That, in view of the necessity of conserving State and Federal indemnity funds, we heartily commend the action of the Insti- tute of American Meat Packers in recommending that all pack- 514 ASSOCIATION NEWS ing companies and local butchers pay as much for reacting cattle that pass food inspection as for healthy cattle of the same quality sold on the open market, and urge also that conservative ap- praisement be made on all reactors. That the association unqualifiedly endorse and recommend that. the strictest attention be given to the conservation of all funds appropriated by counties, States and Federal Government, and any other agencies contributing to the tuberculosis eradication campaign. A motion was passed giving the Executive Committee author- ity to change the dates of the meeting if it deems advisable, to commence on Wednesday of the week of the International Live- stock Exposition instead of on Monday as in the past. Another motion was passed that an evening be designated dur- ing next year’s meeting for a joint session with the National As- sociation of Commissioners, Secretaries and Departments of Ag- riculture, and National Association of Marketing Bureaus. The convention passed an amendment to the accredited herd plan which permits accredited veterinarians to make the pre- liminary tests of herds in the process of accreditation, no in- demnity to be paid by the Government for cattle condemned in such tests. After the herd has shown one clean test the final test must be official and must be a combination test by the subeu- taneous, intradermic and ophthalmic methods. This will permit of greater speed in the work without lowering either efficiency or thoroughness. j In connection with the report of Dr. L. E. Northrup, chair- man of the Committee on Finance, a motion was passed in effect that the matter of publishing the Report of the Twenty-fifth Annual Meeting of the Association be left to the Executive Com- mittee of the organization and that the committee be authorized to borrow money, if necessary, to have the report published, and in case of a deficit, to report it to the association at the next meeting. The election of officers for the coming year resulted as follows: President, Dr. T. E. Munce, State Veterinarian of Pennsylvania, Harrisburg, Pennsylvania; vice-presidents, Dr. W. C. Simmons, State Veterinarian of Kentucky, Frankfort, Kentucky; Dr. B. F. Davis, State Veterinarian of Wyoming. Cheyenne, Wyoming; Dr. Edward Records, Director of the Nevada State Veterinary Control Service, Reno, Nevada; Hon. J. H. Mercer, Kansas | pound eb 3 | ASSOCIATION NEWS Livestock Commissioner, Topeka, Kansas; Dr. O. H. Eliason, State Veterinarian of Wisconsin, Madison, Wisconsin; secretary, Dr. O. E. Dyson, 5451 Woodlawn Avenue, Chicago, Illinois. At present the membership of the U. S. Live Stock Sanitary Association numbers 825, including 31 admitted at the last meet- ing. It seems that the small membership is probably due to the fact that many veterinarians and others are laboring under the mistaken impression that only those engaged in national or State regulatory work are eligible to membership. Section 5 of the Constitution of the association reads as follows: ‘* Any person engaged in livestock sanitary work for Federal, State, Territorial, county or municipal governments shall be eligible to membership in this association, and any other person interested in livestock sanitation may be elected to active mem- bership upon the recommendation of the executive committee and a two-thirds vote of the members present. ’’ The membership is much lower than it should be considering the purposes of the association. Each member should regard it a personal duty to put forth earnest efforts during the coming year to increase the enrollment in this useful and important organization. A SUCCESSFUL TUBERCULOSIS ERADICATION CONFERENCE The Tuberculosis Eradication Conference of State, County, Municipal and Federal Employees engaged in tuberculosis eradi- cation work and others interested in the livestock industry, which was held in Chicago, Illinois, on November 25 and 26, 1921, was unusually successful. This is evidenced by the very large attendance of 560 persons, who maintained their interest throughout the various sessions. A well arranged program was offered, commencing on the morning of the first day with an address of weleome by the Honorable B. M. Davison, Director, Department of Agriculture, Springfield, Illinois. Following this, Dr. John R. Mchler, who presided, explained the objects of the conference and described some of the activities that should receive most careful consideration. Following this, Dr. E. C. Schroeder, Superintendent of the Experiment Station of the Federal Bureau, gave an interesting résumé of the experi- mental work conducted at the station relative to the problem of 516 ASSOCIATION NEWS controlling bovine tuberculosis. Dr. W. F. Crewe, President of the U. S. Live Stock Sanitary Association, followed with a pointed argument relative to cooperation between all of the parties interested in the total eradication of the disease. At the afternoon session, Dr. L. Enos Day, of Chicago, IIL, gave carefully prepared statistics relative to obscure lesions in bovine tuberculosis and illustrated them by a series of beauti- ful mounted specimens. One of the most interesting talks of the session was that presented by Mr. A. J. Glover, Editor of Hoard’s Dairyman, who spoke on ‘‘ The Editor and the Breeder.’’ Mr. Glover’s talk was well received, as he has both personally and editorially forwarded the movement in every way possible. Dr. G. E. Corwin, Deputy Commissioner on Domestic Ani- mals, of Connecticut, speaking on the specifications under which the intradermic test should be made, gave statistics relative to delayed reactions following the injection of tuberculin by this method. Considerable discussion followed this paper, which re- sulted in a motion by Dr. W. J. Butler, of Montana, that the question of the technique and comparative reliability of this and other methods of tuberculin testing be referred to the Bureau of Animal Industry with the view of obtaining extensive sta- tistics for presentation at the next year’s conference. Mr. James Brown, a breeder of Shorthorn cattle, gave a prac- tical talk from the viewpoint of the stock raiser, on the eradica- tion of tuberculosis from a purebred herd. Another talk of much interest to the conference was that of combination tuber- culin test methods by Dr. L. B. Ernest, of the Federal Bureau. The last speaker of the afternoon session was Dr. Isaac Abt, Professor of Pediatrics, Department of Medicine, Northwestern University, who spoke on clinical studies relative to tuberculosis of children. This address was of more than usual instructive- ness because of the new facts presented by one recognized as an authority, and also because of the firm stand taken on the danger of bovine tuberculosis to children. On the morning of the second day, Dr. J. G. Ferneyhough, State Veterinarian of Virginia, gave a very interesting talk on the results obtained by returning aceredited herds to the ac- credited veterinarian. Mr. Thomas Wilson, President of the Institute of American Meat Packers, through his representative, Dr. Fred Eagle, contributed a well prepared paper concerning the economic importance of eradicating tuberculosis. Follow- ASSOCIATION NEWS 517 ing this, Mr. Everett C. Brown, President of the National Live Stock Exchange, through his representative, Professor H. R. Smith, gave interesting data to show the interest his organiza- tion had in the completion of this nation-wide problem. Hon. E. S. Brigham, Commissioner of the Vermont Department of Agriculture, spoke on the subject of 27 years’ experience with tuberculin testing, and showed the possibilities of an intensive campaign, once it is properly organized and started. The breeders of purebred cattle were again heard from throuch Mr. L. A. Campbell, President of the American Aberdeen-Angus Breeders’ Association, who told of the advantages of an ac- credited herd. Following Mr. Campbell, Dr. C. E. Cotton, Sec- retary, Live Stock Sanitary Board, St. Paul, Minn., gave infor- mation relative to the progress of the control work in his State. At the afternoon session Dr. D. F. Luckey, State Veterinarian of Missouri, told of the progress of the work in that State, where at the present time they have 20 counties under the area plan of eradication. This was followed by a talk from Dr. T. S. Rich, inspector in charge of tuberculosis work in Michi- gan, who gave the details of the recent intensive area campaign conducted in Hillsdale County, Michigan. Dr. M. Jacob, of the University of Tennessee, submitted a report of his com- mittee relative to the plans for establishing tuberculosis-free areas. Of especial interest to the official livestock sanitarians present was the address of Dr. J. G. Townsend, veterinary prac- titioner of Racine, Wis., who gave a clear and definite opinion of the place the general practitioner should occupy under the accredited-herd plan. The most instructive and probably the most important address of the entire conference was that pre- sented by Dr. W. A. Evans, of the Health Department of the Chicago Tribune, who spoke on Why Health Departments Are Interested in the Eradication of Bovine Tuberculosis. Dr. Evans’ conclusions were of such importance and so well presented as to meet the needs of those in attendance at the conference better than any other similar material which has been afforded those engaged in the movement. The following quotation from his talk is of such vital importance to both the medical and the vet- erinary professions as to lead to its being given great prominence. He stated: ‘‘The relationship between the human and bovine tubercle bacilli leads health departments to the opinion that while programs for the repression of human tuberculosis, which 51 ASSOCIATION NEWS CO take no note of tuberculosis in other animals, may be successful, the time to strike for repression of human tuberculosis can not come until the program for control of bovine tuberculosis is well advanced.’’ This is food for thought for all interested in the control of the Great White Plague. 0 2 5 2 14 23 N. Y. State (Cornel))......... 14 ils vAT Ae 2 Oe ye! (5) 75 NoriheDakota a= 22s. ee 3: Bie) a eee me ee | Wen eae 6 6 Ohio ere Be ee 14 13 25 SD eee Si, e106 Olela ona oe ee A eee, I ee eae ee Z, 4 Ontario, Canada 2-2 ils: 22, 35 1S ae 88 88 Rennsyivania ” elie ee id b 5 8 fill 31 30 SizyJeseph: seve? 2s 8 YA 35 LO sisi 74 102 Texas) 22. store a eae i 2, Avs MPR, 2, 14 Wnited“Statess=. 3 4 9 Ae eee 20 30 Washington State .................. Ul 2 a Gui C.® 22 23 Totals J See toe see OA IE EBL FANS 16. S799 oS Totals 292022 beet.) 18210281, USOT 1296 » eile 2-3 975 1 Agricultural colleges giving two years in veterinary medicine. North Carolina has no students this session. THE VETERINARIAN AND PUBLIC HEALTH ‘““THE Veterinarian Contributes to Public Health’’ is the title of an interesting article by J. Howard Beard, M. D., University of Ilhnois Health Service, which appeared in the June 15 issue of the popular publication, The Nation’s Health. In this article Dr. Beard discusses tuberculosis, anthrax, rabies, foot-and-mouth disease, milk sickness, pyogenic infec- tions, endoparasitic infestations, and other diseases to which man and animals are alike susceptible. An interesting statement refers to the relation of veterinarians to the physicians, whom Dr. Beard designates as ‘‘allies in scientific research.’’ The following quotations are from the article in The Nution’s Health: “‘Man domesticated the horse, the cow, the goat, the sheep, and the hog, which have fed and clothed and, in the case of the horse and ox, have borne him from place to place. They have broken and fertilized the soil which has grown grains, vege- tables, and fruits more abundantly. Yet these noble animals. when ill, have proved to be the worst enemies of man. They have destroyed him with tuberculosis, killed him with anthrax, cut him off with glanders, filled his muscles with trichine, EDITORIAL 539 racked his body with Malta fever, and loaded his intestines with tapeworms. They may become so dangerous to him when in- fected with anthrax that their meat, milk, hides, hair, or bristles may cause his death. ‘““That man should have some of the roses and avoid the thorns, that these best friends should feed and clothe him to- day and not destroy him tomorrow, is the contribution of the veterinary surgeon to public health. In his efforts to protect and to save animals, the veterinarian has consciously and un- suspectedly pointed to hidden paths by which man has been able to attain an average life nearer the normal limit of three score andten. * * * ““The veterinary surgeon has contributed to public health by caring for animals infected with diseases which occur pri- marily in man; secondarily in animals. Both cattle and hogs may contract human tuberculosis and later transmit it to man. Cowpox and horsepox have been known for centuries. They probably originated with man and, according to many expert opinions, are modified variola or smallpox. In 1796, Jenner showed they are capable of producing an immunity to small- pox when inoculated in man. While the veterinarian has ren- dered distinct service in controlling these diseases in animals, in the case of man, more accidental inoculations would reduce smallpox among the unvaccinated without subjecting them to the danger of disfigurement of variola. * * * ‘‘Veterinary science advances public health by stamping out endoparasitic diseases in livestock and in domestic animals, for which man may be the accidental, intermediary, or the only host. The more important internal parasites from the stand- point of public health are trichina, Taenia solium, T. saginata and T. echinococcus. * * * ‘‘Discoveries in the field of veterinary science, with rare ex- ceptions, have been helpful in the domain of medicine. Study of splenie fever in cattle and the demonstration of the relation of the tick to its transmission gave a new conception of the role of insects in the spread of disease and blazed the way for a better understanding of Rocky Mountain spotted fever, relapsing and South African tick fever in man. Investigation of surra in horses and nagana in cattle offered fertile suggestion in the study of human trypanosomiasis. Pasteur’s immunization of chickens 540 EDITORIAL against cholera and cattle and sheep against anthrax are the forerunners of inoculation against typhoid fever, cholera and plague. The world owes a great deal to the milkmaid who told the medical student, Edward Jenner, ‘I can not take smallpox because I have had cowpox.’ Her information led to the discov- ery of vaccination which has saved millions of human beings from death or disfigurement. Incidentally it reduces the cases of cowpox among domestic animals. ‘‘The study of malnutrition, and its treatment in livestock and fowl has proved very helpful in dealing with scurvy, pellagra, rickets, and beriberi, and metabolic disease in man. Progress in animal feeding has usually created new possibilities for the better nutrition of man. Investigations of tumors in animals have shed additional light upon cancer. ‘“‘The veterinarian’s work with young animals has aided the pediatrician in feeding and caring for infants. Treatment of abnormal parturition and of post-partum illness in animals has in many instanees offered helpful hints to the obstetrician which have extended the boundaries of his subject and resulted in throwing greater safeguards around childbirth. ‘Tn the battle against disease, the veterinarian and the physi- cian are complements and allies. The former strives to prevent sickness among animals and to protect man; the latter seeks to preserve the health of man and incidentally saves animals. The one alleviates the sufferings of noble brutes; the other brings relief to his fellows. Both covet the glorious title of benefactor of humanity.”’ Although veterinary medicine is yet in its infancy compared with human medicine, its important achievements in the field of research have ressulted to the advantage of all concerned in establishing a relationship of mutual respect and. confidence be- tween scientific investigators in these allied fields of usefulness. When we pause to consider the intimate relationship between animal and human maladies, it seems strange that there has not developed more rapidly a better understanding and closer work- ing relation between practitioners in human and veterinary medicine. There is no doubt that they could be of mutual as- sistance in practice if a closer contact were established. The essential contact between veterinary and human practi- tioners is possible through. existing associations and popular sci- ——— ain ie EDITORIAL 541 entific publications. Medical and veterinary associations now hold meetings at regular intervals, but there has been little effort on the part of either to encourage affiliation. Seldom do physi- cians attend veterinary conferences and rarely do the names of veterinarians appear on the programs of the meetings of medical associations. It is true that several veterinarians are members ot and not infrequently take part in the programs of the Amer- ican Public Health Association, Society of American Bacteri- ologists, National Association for the Prevention of Tuberculosis, Society of Experimental Medicine and Biology, American Asso- ciation for the Advancement of Science and similar orgariza- tions that welcome veterinarians to their meetings, but unfor- tunately too few of them avail themselves of such opportunities. It would be beneficial to both professions and to their clientele if the larger medical and veterinary associations were to include in their programs a short period for the discussion by physicians and veterinarians of subjects that are of mutual interest. It is hoped that the A. V. M. A. will be one of the first to make a move in this direction and adopt such a policy, not for any one year, but for each annual convention. Dr. Beard is to be complimented on his splendid article and it is hoped that more such valuable contributions from physi- cians and veterinarians will appear in publications devoted to problems relating to disease, health and sanitation. The Veterinary Journal (British) has reprinted in full, with extended favorable editorial comment, the paper by Dr. David S. White on ‘‘Our Profession,’’ which appeared in our pages in April, 1921. The average man would rather put on the regalia of some order and be known as the supreme grand hoozis than to know the contents of an encyclopedia or be a Ph. D.—Journal of the American Medical Association. ————_——___--—— The Scottish Farmer reviews approvingly what it character- izes as ‘‘a humorous, striking, and essentially American circu- lar,’’ issued by the Horse Association of America, which ‘‘ef- fectively upsets the theory that the horse is doomed by the in- creasing use of motor power,”’ BUREAU OF ANIMAL INDUSTRY INVESTIGATIONS ON BOVINE INFECTIOUS ABORTION ! By E. C. SCHROEDER Superintendent of Experiment Station, United States Bureau of Animal Industry, Bethesda, Maryland MY TASK is a discussion of the investigations of the Federal Bureau of Animal Industry on the common and widespread evil known as bovine infectious abortion. As the investigations have beerlong in progress and quite active in recent years, it may be well to say in advance that it will be impossible in the time at my disposal to enter into minute detail concerning them. And probably it is fair to assume that a minutely detailed account of the arduous, time-consuming and patience-trying technical work that has been done would prove less interesting or useful than a simple presentation of the facts that have been revealed or confirmed, together with a reference to their economic significanee, provided enough is said about the evi- dence on which they are based to show that they are facts in reality and not merely in name. Efforts to control infectious diseases before their etiology is known, at best, are general and commonly as wnpromising as the chances that a marksman, with a vague idea of the direction in which he should aim, will make an effective shot in the dark. If enough ammunition is burned a lucky hit is occasionally made, but usually the ammunition is burned in vain, and the noise, smoke and confusion, even if stray shots do not prove uninten- tionally destructive, profit and please no one. What the marks- man needs is light, and the light needed to combat infectious diseases is a reasonably clear knowledge of their etiology. The prime etiological factor of bovine infectious abortion was discovered by Bang and Stribolt long before the more active in- vestigation of the evil was undertaken by the Bureau of Animal Industry, and the discovery had been confirmed by McFadyean and Stockman in England, and MacNeal and Kerr and Ward Giltner and Good in America. But etiology is more than the recognition of the causative microparasite of a disease, and it remained to be determined how and when the parasite, the Bang 1Presented at the twenty-fifth annual meeting of the United States Livestock Sanitary Association, Chicago, Ill., Nov. 29-30, 1921, 542 — a INVESTIGATIONS ON BOVINE INFECTIOUS ABORTION 543 bacillus, enters and leaves the bodies of its hosts or victims; whether it occurs elsewhere in nature than in the bodies of its victims; on what natural conditions its multiplicatiom and per- petuation depend, ete., ete. Though some of the facts were known, so much remained in doubt that the available data led to wholly contradictory conclusions and fruitless controversies, and consequently when the question was asked, ‘‘ How shall we combat abortion disease?’’ the answers ranged from a modest ““‘T don’t know’’ to suggested flushings and douchings that were apt to cost more per animal than the value of ordinary eattle per head, and the enactment of stringent laws and regula- tions which would have been more annoying and expensive to eattle owners than beneficial to their herds. Confronted with this uncertainty and confusion, it required no prophet to foresee that an investigation of bovine infectious abortion would fail of its purpose unless vigorous efforts were made to amplify and clarify the existing etiological knowledge of the disease, and therefore etiological studies were at once planned and undertaken. An abundanee of work also was done relative to treatment, general as well as specific; but I will speak of the etiological investigations first. THE OCCURRENCE OF THE BANG BACILLUS IN THE Bopies or CATTLE The Bang abortion bacillus, like the tubercle bacillus, evi- dently is an obligatory parasite; it is known to multiply nowhere in nature but in the bodies of its hosts. In culture tubes, carefully sealed to prevent drying, it has been kept alive in the Bureau laboratories nearly three years; and in placentas of aborting cows, exposed under fly screens in shaded places in the woods during the colder months of the year, it has remained alive, in rare instanees, as long as four months. In material dis- charged from the bodies of aborting cows, unless the conditions are exceptionally favorable for its preservation, the bacillus generally dies in less than a month, and its death is greatly hastened by sunlight. The weak resistance of the nonsporulating microparasite against natural germicidal forces implied that it could not perpetuate itself and serve as the cause of a wide- spread plague unless some special provision had been made by nature for its preservation, and the most reasonable provision 544 E. C. SCHROEDER to look for was its long-continued presence in a dormant or a partly dormant state in the bodies of its hosts. It was known that the bacillus oceurs in the placentas of aborting cows and in different portions of the bodies of aborted fetuses. The investigations of McFadyean and Stockman indi- cated that it disappeared from the uterus within approximately a month after an abortion. The discovery had been made inde- pendently by the Bureau and by Smith and Fabyan that it occurs in the udders and milk of some infected cows. It re- mained to be determined how long it persists in the udder and, more definitely, how long it persists in the uterus and other portions of the reproductive organs. It remained to be deter- mined whether it occurs in other parts of the body than the reproductive organs and the udder, and what its presence in other parts of the body signified. It also remained to be deter- mined whether it occurs in the bodies of calves, heifers and bulls. Today we know that the Bang abortion bacillus may, in rare cases, persist in the uterus two months after an abortion or a parturition, but that, as a rule, it does not persist more than two to three weeks; that it may persist in the udder and milk for greatly varying periods of time, from a few weeks to six or seven years; that the udders of more than 60 per cent of infeeted cows at some time harbor the bacillus; that when it is present in the udder it invades the uterus in a large proportion of cases during pregnancy and may be very abundant though the pregnancy is seemingly normal and ends in an apparently normal parturition; that it occasionally attacks and can be found in the reproductive organs and seminal fluid of bulls; that it may be present in the stomach fluids, livers and gastro- hepatic lymph glands of newly born, viable calves, and that all attempts to find it in the bodies of cows elsewhere than their udders, their uteruses during gestation and shortly after an abortion or a parturition, and the lymph glands associated with these organs, have failed. It does not require much time or many words to state these facts. How much work was required to secure them may be evident to practical investigators, and even they are apt to fall short in their estimates. For example, the short statement that ‘abortion bacilli oceur in the bodies of newly born, viable calves’’ rests on tests in which eleven calves were used, as follows: The calves were removed from their mothers, abortion- INVESTIGATIONS ON BovINE INFECTIOUS ABORTION 345 infected cows, immediately after delivery, and not permitted to have access to anything that could lead to the postpartum inges- tion of infected material; they were killed and sectioned with strict aseptic precautions, and tissues were gathered from all parts of their bodies for cultural and animal inoculation tests, and later, when positive results were obtained, they were con- firmed by the subinoculation of experiment animals and culture tubes. If positive results had been obtained with the first two or three calves it would have settled the matter; but this was not the ease; and when the results are negative the number of animals examined must be multiplied before the evidence is sufficient to give reasonable stability to a conclusion based on it. So with the brief statement that the abortion bacillus occurs nowhere in the bodies of cows but in their udders, pregnant or recently pregnant uteruses and associated lymph glands. The Bureau investigators who obtained the data that prove this statement can tell about the arduous task it was to kill and section abortion-infected cows so that their blood, spleens, livers, lungs, kidneys, serous membranes, synovial fluid, bone marrow, brains, spinal cords, muscles, uteruses, vaginas, Fallopian tubes, ovaries, ete., and lymph glands from all portions of their bodies could be tested. Not one or two cows, but a good many of them, because negative results require that the number of subjects ex- amined must be large enough to insure that the exception which often is supposed to prove the rule has been eliminated. Regarding the calves found to harbor abortion bacilli at birth—two out of eleven examined—it is noteworthy that one was the offspring of a cow that has never. aborted and was recognized as a carrier and spreader of abortion bacilli only through the use of biological tests. The examination of the bodies of calves for abortion bacilli was not confined to the eleven newly born ealves, because the discovery that viable calves may, harbor abortion bacilli at birth made it important to determine how long the ealves of infected cows may remain earriers of the bacillus, particularly as the untenable hypothesis that ‘‘abortion bacilli may lie dor- mant in the bodies of cattle from birth until sexual maturity and then cause abortions’’ had been widely promulgated. We know now, as the result of a large number of tests, that young cattle rarely harbor abortion bacilli in their bodies, and that, when they do so, they react to abortion tests. Many 546 E. C. SCHROEDER ealves react to such tests during the first weeks of their lives, but the reactions rapidly decline and disappear even if the calves are suckled by dams with infected udders, and, if they are protected against exposure to abortion infection after they are weaned, they become normal, healthy producers of calves when they reach sexual maturity. As far as the Bureau’s work has gone, the calves of abortion- to abortion tests during the first weeks of their lives, and irre- spective of whether they were suckled by cows with clean or with infected udders, if they are protected against infection after they are weaned, are neither more nor less susceptible to abortion disease when they reach maturity than the calves of normal cows. This would indicate, if herd immunity against abortion disease actually develops, that it is less due to anything that calves inherit from their dams or acquire by ingesting infected milk during the milk-drinking periods of their lives, than to a fairly continuous exposure to abortion bacilli from birth until maturity, and possibly to their exposure particularly during the last two to three months before conception. The significance of the exposure of cattle to infection with abortion bacilli during presexual life is a line of work that was greatly hampered by lack of funds. It was planned and well under way before our country entered the war. What happened to the cost of labor and feed after the war was entered is too well known to require discussion here, and what effect this had on the Bureeu’s experimental investigations may be judged when it is known. that the appropriations made for them did not take the reduced purchasing value of money into account. Work requiring a certain number of animals, planned when hay was plentiful at $20 a ton and other forage equally cheap, was impossible without greatly increased funds when hay was scarce at $40 a ton and other forage had increased propor- tionately in cost. The Bureau had no choice; it would have pre- ferred to retain all its experiment animals and to continue its investigations undisturbed, but the pecuniary obstacles were too great to be mastered by scientific enthusiasm, and so it was forced to resort to painfully severe retrenchment. Many animals that had acquired great value because of the treatment to which they had been subjected and because their origin and history were fully known, and which it was desirable to keep eo a SiS atime ll alts be 0 ll a Sh i Na AN Se aa, al a as Sieeietiecie die ee >> INVESTIGATIONS ON BovINE INFECTIOUS ABORTION 547 under observation, had to be disposed of, and, though no one was really to blame, the sorrow and regret this caused will not soon be forgotten. The work on which the conclusion is based that the nonpreg- nant uterus is not a habitat of the abortion bacillus is somewhat varied in character. The first tests dealt with infected cows that had aborted or given birth to calves. Beginning directly after the abortion or the parturition, material was collected periodically on sterile swabs from the uteruses of such cows, and studied for the presence of abortion bacilli. The studies showed—and the number made cannot be stated in dozens and scores, as they amount to many hundreds—that abortion bacilli as a rule disappear from the uterus in which they were abundant at the time of an abortion or a parturition in from two to three weeks, though occasionally they persist somewhat longer and in exceedingly rare instances as long as nearly two months. The seeond line of tests concerned cows into the uteruses of which cultures of abortion bacilli had been injected; the third, cows that were killed and all portions of their uteruses tested for abortion bacilli possibly hidden beneath the surface; and the fourth, cows that received copious intravenous injections of abortion bacilli and the uteruses of which were studied during subsequent estrual periods. It was reasonable to conclude, though abortion bacilli could not be collected from a uterus on a cotton swab, that they might be present in or beneath the mucous membrane, or somewhere in the cotyledons, Fallopian tubes or ovaries, but this did not prove to be the case. Likewise, since the pregnant uterus is the favorite habitat of the bacillus, it was reasonable to believe that special activity of the reproductive organs would better enable it to maintain itself in the uterus, and that, therefore, the estrual period deserved special attention. It is fortunate that this period does not help the bacillus to live and multiply in the uterus, because, if it did, we would be forced to conclude that all cows with infected udders are prolific disseminators of abor- tion bacilli via their vaginas during a number of days every. month. The discovery that bulls may harbor abortion bacilli in their reproductive organs is also the product of industrious applica+ tion; that is to say, it was not a fortunate stumbling on a fact. 548 E. C. SCHROEDER As the belief was widely entertained that infectious abortion is carried from cow to cow by the bull, and that he introduces it at the time of copulation directly into the uterus, it was de- sirable to determine on what part of his sexual organs the bacilli occurred and how long they remained virulent. Hence, material was collected from many bulls shortly after they had served cows that reacted to abortion tests and were known to have infected udders, and this material was tested for abortion bacilli. The results in every case were negative, and it was found that the bacilli did not survive long even when pure cultures were introduced into the bull’s sheath. This work was followed by postmortem examinations of reacting bulls, and with tests of all portions of their bodies for abortion bacilli in the manner in which the bodies of reacting cows had been tested, and the organism was finally discovered in an epididymal abscess. Since then a number of bulls have been artificially infected with abortion bacilli, and studies have been made relative to the frequency with which bulls react to abortion tests, and the frequency with which lesions chargeable to abortion bacilli occur in the reproductive organs of reacting bulls; also studies which conclusively proved that bulls with infected reproductive organs may expel abortion bacilli with their seminal fluid. The studies on the frequency with which bulls react to abor- tion tests dealt with several hundred bulls sent to an abattoir near Washington for slaughter. The bulls were first tested, and then, if they reacted, their genital organs were obtained and ex- amined for lesions and abortion bacilli. Approximately 10 per cent of the bulls tested reacted, and approximately 10 per cent of the reacting bulls showed lesions of the reproductive organs from which abortion bacilli were isolated. The value of these studies is not that they give us a measure of the proportion of bulls that react positively to abortion tests or the proportion of reacting bulls that are carriers of abortion bacilli. The number of bulls tested, about 325, is too small to serve this pur- pose, to say nothing of the fact that bulls removed from herds and sent to the butcher are not representatives of those retained in herds to serve cows. Their value lies in the fact that they show that abortion bacillus disease of the bull’s reproductive organs is not a wholly unique affection which practically may INVESTIGATIONS ON BOVINE INFECTIOUS ABORTION 549 be ignored, but an important condition that must be taken seriously into account in our efforts to combat infectious abor- tion, since it has been proved to be associated with the contami- nation of the seminal fluid. Mopes or DISSEMINATION Precise knowledge of the habitat of a parasite in the body of its host aids greatly in determining how it gets out of one host and into another; or, in other words, if the parasite is a pathogenic microorganism, how the disease it causes spreads from victim to victim. The knowledge that the abortion parasite lodges nowhere in the Hving bodies of cattle but the udders and pregnant and re- cently pregnant uteruses of cows, the reproductive organs of bulls, and the digestive tracts and livers of newly born calves, and the lymph glands more or less intimately associated with the named organs, at once calls attention to the channels through which it is expelled. From the udder the bacillus is expelled through the teat with milk, from the uterus with aborted fetuses, the placentas and discharges following an abortion or a parturition, and from the sexual organs of bulls with seminal fluid and other discharges. These three modes of expulsion have been definitely proved, and no doubt can be entertained about them. That the bacillus also may be expelled with the alvine discharges of infected calves and calves that drink infected milk seems probable but has not been definitely proved. It is a question on which experimental studies have been made, but the data obtained are not sufficient to justify a final answer. But, even if the answer eventually is affirmative, this mode of dissemination could hardly be charac- terized as important, as it would be confined to calves with infected mothers and would be closely limited to the time when the mothers are more or less actively, expelling infected material from their uteruses. The udder becomes infected in upward of 60 per cent of all infected cows, and the time during which it remains infected varies from a few weeks to six or seven years. An important fact about cows with infected udders is that in a large proportion of cases the infection extends to the uterus during gestation. Among the cows with infected udders thus far examined about 550 E. C. SCHROEDER one-half were proved to harbor abortion bacilli in their uteruses at the time of parturition. An infected udder does not mean that a cow has aborted or will abort, or that she has shown or will show symptoms of abortion disease, but it does mean that she is a dangerous animal to introduce into an abortion-free herd of cattle. For- tunately, after having made hundreds of tests, Bureau investi- gators have not yet found a cow with an abortion-infected udder that did not react in an unmistakable manner to the agglutina-, tion and complement-fixation tests for bovine infectious abor- tion. The expulsion of abortion bacilli from the uterus via the vagina, as has already been stated, is not of long duration. It requires that the products of all abortions and the by-products of all parturitions and discharges following abortions and par- turitions should be treated as dangerous material. The discharges from the male sexual organs may be dangerous when the bull is permitted to run with the herd, and hence, in the control of bovine infectious abortion, it is desirable that he should have a separate pen, away from the cows, and that he should be permitted to serve cows only on neutral ground, or ground to which cattle do not have access at other times than during that of service. It may also be wise to segregate cows after they have been served by possibly infected bulls until all danger that infected seminal fluid may leak from their vaginas has passed, and to fasten them during such segregation in a way that will prevent them from eating anything that may have become soiled with leakage from their vaginas. These statements concerning the bull may sound curious to those who continue to hold the once widely entertained hypothe- sis that bovine infectious abortion is transmitted from cow to cow largely by promiscuously used, unclean bulls. Neither the investigations of the Bureau nor other data on the subject support this hypothesis, which, in fact, has little support other than a general inclination to believe that the channels of infec- tion for microscopic diseases must be the passages through which the attacked portions of the body communicate most easily or directly with its exterior. | It is regrettable that the time is too short to give a detailed account of the work that lies behind the statements this paper ? i) | OV pom INVESTIGATIONS ON BOVINE INFECTIOUS ABORTION contains. If it could be given it would inspire confidence and show why I am privileged to speak with unfaltering assurance. Take a problem, for example, like the recurrence of abortion bacilli in the uterus of a cow with an infected udder at each of several successive, apparently normal parturitions. Think of the tests; milk tests, bloods tests, tests of placental material and uterine discharges, tests of calves, ete. Think of the watchful- ness to prevent intercurrent infection from becoming a source of error; think of the experiment animals and culture tubes inoculated ; think of the subinoculations of animals and culture tubes to validate the use of the results obtained as evidence. And, bear in mind, this work was not done with one or two animals, but with all the accessible cows that could be used as reliable material, until the number was large enough to justify the conclusion that at least half of all cows with infected udders have infected uteruses at the time of calving. To reveal facts and to secure their recognition and use re- quires time and work and money; but facts are eternal things and the stock the world has on record is a criterion of its civi- lization; and probably the conviction that this is true, coupled with a desire to be real agents in the world’s progress, has bound many able workers loyally to their poorly remunerated service in research establishments. CHANNELS OF INFECTION Microscopic as well as macroscopic parasites have favorite habitats in the bodies of their hosts, and the location of the habitat in most cases has nothing to do with the channels through which the host is invaded. Though this is a matter of common knowledge, many investigators of microparasitic dis- eases seem to cling to the fallacy that a close relationship exists between the attacked organs and the nearest channels from the exterior of the body through which they may be reached. Hence it is not surprising that it should have been assumed, or that the assumption should have gained wide credence, that the vagina is the common portal of entry for the microparasite of bovine infectious abortion, as the favorite habitat of the parasite is the pregnant uterus, where it attacks primarily neither the parent nor the offispring, but rather the medium through which the two are in communication. It was early recognized by Bureau investigators that this 552 E. C. ScHROEDER assumption lacked proper support, and that, if it were true, the community-owned or association-owned, more or less promis- cuously used bull would prove an ideal agent to facilitate the spread of abortion disease among cattle. At the same time it was apparent that the use of a relatively small number of well- bred community or association bulls was vastly more economical than the use to only a fraction of their capacity of a larger number of individually owned scrub bulls. These and other reasons for studying the role of the bull in the spread of bovine infectious abortion were unmistakably urgent, and prompted the experimental tests of which the following is a brief outline. Cows wholly free from abortion infection were given intra- uterine injections of pure cultures of abortion bacilli, placental material from cases of abortion, and material obtained from aborted fetuses before they were served by a bull; others were served by bulls which reacted positively to abortion tests; others were served by bulls which had shortly before served cows with infected genital tracts, and others by bulls both naturally and artificially infected which were expelling abortion bacilli with their seminal fluid. Quite a number of cows were used, many of which afterwards were proved to be susceptible to abortion disease; but in all cases the results failed to justify, in the least degree, the assumption that cows are infected with abortion bacilli via their vaginas and uteruses at the time of copulation, or that the bull, through copulation, is an agent in the spread of abortion disease. The cows not only failed to abort, but re- mained negative to abortion tests. The total number of calves produced was smaller than probably would have been the case if the uteruses of the cows had not been subjected to unnat- ural treatment and the bulls had not been somewhat deficient in potency because of the diseased condition of their sexual organs; but the cows that conceived passed through their periods of gestation in a normal manner, and the gestations ter- minated in the normal births of normal calves. In each case after service by infected bulls seminal fluid was collected from the vaginas of the served cows and tested for the presence of abortion bacilli, and the bacilli were repeatedly found in the seminal fluid of one naturally and one artificially infected bull. Tn each ease the placentas and uteruses of the cows were tested after parturition, and no abortion bacilli were found. INVESTIGATIONS ON BovINE INFECTIOUS ABORTION 593 Clearly, the uninjured sexual cavities of nonpregnant cows do not serve the abortion bacillus as channels of entry. Whether infection may occur via the vagina after pregnancy has been established has been given little attention by Bureau investiga- tors, mainly because it is not an economically important matter, as such infection could hardly occur elsewhere than in an en- vironment in which the bacilli are very abundant and in which they could easily enter through other channels. Work of other investigators seems to prove that the injection of abortion bacilli into the vaginas and uteruses of pregnant cows leads to abortions. The community or association bull evidently is harmless so far as infectious abortion is concerned, and he is so without elaborate and troublesome disinfection of his genital organs, provided the precautions already suggested—a bull-pen, service on neutral ground, ete.—are observed. As the udders of infected cows in many cases harbor abortion bacilli long periods of time, their transferrence from udder to udder during milking seemed reasonably possible. An experi- ment relative to the matter was made, but the number of animals used was too small and the time they were kept under observa- tion too short to give the negative results obtained full validity, particularly as it was definitely proved that abortion bacilli introduced into the udder, through the teat with a milking tube, soon reach the uterus of a pregnant cow and cause abortion. - This is a line of studies that was interrupted by inadequate funds, but the problem with which it dealt is one that is not urgently in need of a solution, because the transferrence of infection from cow to cow through milking would be practically impossible elsewhere than in an environment in which infection through other channels would certainly occur. The one mode of infection—and I speak in this case of modes that require no artificial practices—that gives positive results is the ingestion of abortion bacilli. When a susceptible, preg- nant cow is permitted to ingest abortion bacilli, either pure cultures or material from cases of abortion, she rarely fails to become a victim of the disease. In a large proportion of cases she afterwards aborts and reacts positively to abortion tests, and a large proportion of the cows that do not actually abort after having ingested abortion bacilli show other signs of the 554 E. C. SCHROEDER presence of the evil, such as abortion bacilli in the placenta and the uterus at the time of parturition, the production of a weak calf, the occurrence of the bacilli in the udder, ete. Ingestion, to judge from the investigations of the Bureau and other available data, seems to be the natural mode of entrance for the abortion bacillus into the bodies of its victims, and other conceivable natural modes of entrance, though they may not have been definitely disproved, plainly lack experimental evidence that proves their existence. Letters are received occasionally from cattle owners describ- ing in detail how the introduction of a new bull into their originally abortion-clean herds was shortly followed by a seriously large number of abortions, with absolutely no discoy- erable source of infection but the new bull, regarding which it was learned too late that his history could be traced to an abor- tion-infected herd. In cases like this we may feel confident, if all other sources of infection have been fully excluded—quite a difficult thing, by the way, when we deal with a common, widespread, insidious evil like bovine infectious abortion—that the bull was not only affected with abortion bacillus disease of his reproductive organs, but was also permitted to associate freely with the cows, so that leakage from his penis would lead to the contamination of their food and drink and to their infection through their digestive tracts. TESTS FOR ABORTION DISEASE The control measures indicated by the nature of the abortion bacillus, its habitats in the bodies of cattle, the manner and time of its expulsion, and by the channels through which infec- tion occurs, are relatively simple. The real difficulties begin, as naturally would be expected with a disease in which the seem- ingly healthy carriers of its prime cause are exceptionally numerous, when the distinction between dangerous and safe animals must be made, and this brings us to a consideration of abortion tests. Among the various tests which have been devised, two, the agglutination and the complement-fixation, alone possess a serv- iceable degree of efficiency, and the Bureau has found, after having made thousands of comparisons, that on the basis of reliability there is no choice between the two, but that the agglu- tination test, because of its much greater simplicity, is the one Be Me wt Or INVESTIGATIONS ON BovINE INFECTIOUS ABORTION that should be preferred, and this is a matter on which most investigators are in accord. These tests have been criticised because they do not show that a cow will abort, and because some cows do not react after they have aborted, and for a number of other reasons; but such crit- icisms lose most of their weight if we view them with a ra- tional comprehension of what the tests are and of the kind of information they can be expected to give. To expect that an agglutination or a complement-fixation test for bovine infectious abortion should indicate that a cow will abort would be similar to requiring of the tuberculin test that it should show whether an animal will succumb to tubercu- losis within several months; and to expect that every cow that has aborted should react to infectious abortion tests would be like expecting the tuberculin test to show the presence of any disease with necrotic, caseous or caleareous lesions. An abor- tion is, strictly speaking, the supreme or superlative event in bovine infectious abortion, and not an essential event. We may reasonably say that as all children attacked by diphtheria do not die, so all cows attacked by bovine infectious abortion do not abort ; and we may also say that as all diphtheroid lesions are not due to Loeffler’s bacillus, all abortions among cows are not due to the Bang bacillus. The Bang bacillus may enter the body of a cow so shortly before an abortion that a reaction does not occur until some time afterwards, and it is not difficult to believe that in rare instances, owing to recondite or abstruse causes, cows infected with bovine infectious abortion fail to react, just as some tuber- culous subjects fail to react to tuberculin. But on the whole, if the agglutination and complement-fixation tests are properly made, they show with an amazing degree of perfection whether an animal is or has been harboring abortion bacilli in its body. Some of the noteworthy results obtained with the aggutina- tion test by Bureau and other investigators are as follows: If if ean be proved that the udder of a cow harbors abortion bacilli, the reaction will be positive in a dilution of 1 to 200 or more; in reactions obtained respectively with milk and blood serum from the same cow, though they may be equal, that of the serum usually is a little stronger; colostrum from infected cows often reacts in enormously high dilutions; the calves of reacting cows 556 E. C. SCHROEDER often react, sometimes higher than their dams, but the reactions are passive and do not persist long even when the calves are suckled by cows with infected udders; when two tests are made some time apart, a declining reaction, particularly if it is below 1 to 200, signifies that the animal probably no longer harbors abortion bacilli in its body; bulls which harbor abortion bacilli, in all cases so far tested, react positively, ete. A reaction in a dilution of 1 to 200, or 1 to 25, or 1 to any other number, as the expression is used in this paper, means that the bacilli in a definitely measured volume of a standard suspension of abortion bacilli are agglutinated by adding to it an amount of serum which is proportioned to the volume of the suspension as the smaller number is proportioned to the larger ; thus, a reaction in a dilution of 1 to 200 would mean that the volume of the suspension was 200 times as great as the volume of serum added to it, and, in a reaction of 1 to 25, that the volume of suspension was 25 times as great as that of the serum; consequently, if the volume of the suspension was 1 c¢.c., that volume of serum in the one case would be 1 divided by 200, or 0.005 c.c., and in the other, 1 divided by 25, or 0.04 e.c. Reactions vary greatly with material obtained from different cattle that are known to harbor abortion bacilli, and may reach the enormous height, when blood serum is tested, of 1 to 3,200, and when colostrum is tested, of 1 to 25,000. Think a moment of the potency of the specific agglutination agent indicated by these figures. Blood serum and colostrum chemically are com- plex substances of which, at most, a very small fraction can be supposed to be the agglutination agent; and yet the serum at times agglutinates the bacilli in 3,200 times, and the colostrum in 25,000 times, their own volume of suspension. What measure of agglutinating power of the blood serum should be regarded as a certainly positive bovine infectious abor- tion reaction, whether it should be higher than 1 to 50 or as low as 1 to 10, has not been definitely determined and is a subject on which dogmatie statements would be dangerous before all matters relating to the test have been so standardized that a satisfactory comparison can be made between the reactions ob- tained by different investigators. The Bureau investigators are strongly inclined to believe, on the one hand, that even a very low agglutinating property of arate ee eet pes nett oy et hr cll GemenneR treate aes saree or Oo INVESTIGATIONS ON Bovine INFECTIOUS ABORTION blood serum for abortion bacilli should be viewed with suspicion, and, on the other, that a declining reaction, particularly if it drops below and remains lower than 1 to 200, indicates that an animal is safe so far as the dissemination of abortion bacilli is coneerned. TREATMENT AND PREVENTION Treatment of infectious abortion with drugs or chemical agents, either internally or externally, has given little, or no, or only false encouragement. The Bureau’s work in this connection, though not abundant, proves—doubtless a matter of common experience—that the more insidious and chronic a disease is, the greater the chances are that those who attempt to treat it may go astray in their valuation of the events that follow treatment, more particularly if the treatment is given under the supervision of a conscientious, capable person who greatly im- proves the sanitary conditions under which his subjects live, without taking into consideration how much the consequently more hygienic environment may benefit both the affected and the exposed subjects. Unquestionably it is economical to give proper treatment to cows that have aborted, or that do not clean properly, or that are afflicted with other troubles of the reproductive organs and functions; but such treatment should not include attempts to disinfect the uterine and vaginal cavities by irrigating them with strong germicidal solutions, because such attempts will prove futile and can not be made without serious danger of doing harm. The modern and rational idea of wound treatment should be applied to the treatment of the injured or diseased uterus, and that means the removal of dead and foreign material on which saprophytic and facultative pathogenic microorgan- isms can feed and multiply, and not the further devitalization of injured and diseased tissues. Treatment with abortion bacilli, killed and living, alone and in combination with antiserum, has been tried with varying and contradictory results. The Bureau’s work on the subject is re- stricted to the use of killed cultures among pregnant and living cultures among nonpregnant cattle. In one large herd numbering over 1,000 head of eattle, of which 911 remained under observation one year and 453 a second year, the results from the injection of live organisms before con- ae) 558 E. C. SCHROEDER ception were as follows: Treated cattle, first year, 13.12 per cent of abortions; second year, 10.29 per cent. Untreated cattle, first year, 17.7 per cent of abortions; second year, 14.09 per cent. The treated animals in the first year numbered 617 and in the second 311, and the untreated in the first year 294 and in the second 142. Regarding this herd it should be said that it was located a long distance from Washington, and could not be kept under the close, continuous, personal supervision of those who planned the work, that is desirable in an experimental test, and unfortu- nately the uninterrupted services of a trained inspector to imme- diately supervise it failed for reasons over which the Bureau had no control. The live organism treatment, or attempted immunization, how- ever, in both years, though it was not fully satisfactory, evi- dently was not attended or followed by ill effects. In both years the proportion ot abortions among the treated cattle was a little lower than among the untreated, and the percentages of sterility which developed in the two groups during the time they were under observation were practically alike. An examination of the available data on the use of living cultures of abortion bacilli to produce immunity shows three things: (1) That very few tests have been made under strict experimental conditions; (2) that the number of abortions among cattle that have received injections some time before con- ception drops with a noteworthy frequency to approximately 6 per cent, no matter what it may have been before immunization was attempted; and (3) that the per cent of abortions among treated cattle is lower as a rule than among untreated cattle kept under the same conditions. In connection with this statement it may be interesting to record the observations made in a large, privately owned herd of dairy cattle, which the Bureau includes among its investiga- tional material, and to give a synoptic account of one precisely made experimental test.on immunization. The dairy herd numbers about 200 eattle; it has long been infected with abortion, and the current number of abortions was sreatly reduced by raising the heifer calves born in the herd to replace cows as they outlived their usefulness. All the cattle have been repeatedly tested with abortion tests; the proportion INVESTIGATIONS ON BovINE INFECTIOUS ABORTION 559 of reacting cows is relatively small, and the reacting and the nonreacting animals are not permitted to come into contact with each other. In this herd about 6 per cent of the cows have aborted during the past year, and the proportion of aborting cows among those that react is about equal to the proportion among those that do not react. We have here this factor of about 6 per cent which is frequent among abortion-immunized cows. Would it not be pertinent to ask whether it is a figure that approximately indicates the num- ber of abortions among cattle that are due to other causes than the Bang bacillus? We might lightly dismiss this question by saying that we are here face to face with the inefficiency of abortion tests; but let us not be too hasty. It would be surpris- ing if no other causes than the Bang bacillus existed for abor- tions among cattle, animals superlatively artificial because of the kind of selection that has been practiced in breeding them for human, utilitarian purposes without giving too much thought to what nature intended them to be; and it would likewise be sur- prising, when we bear in mind how complex the reproductive functions are, if there were no other causes for abortions among cattle than microparasites. Some of the Bureau investigators have determined to devote much time and study to this phase of the abortion question in the future. And now for the test, which does not include a large number of animals, but was made in harmony with true experimental standards. ’ Twenty-three cattle, part of a drove of 66, not one of which, according to the tests that could be made, was infected with bovine infectious abortion, were divided into three groups. Eleven received subcutaneous injections of pure living cultures of abortion bacilli about two months before they were served by bulls; 4 received repeated injections of killed cultures of abortion bacilli after they had become pregnant; and 8 were retained untreated, as checks or controls. The 23 cattle were equally and similarly exposed to abortion infection; the ex- posure was via the digestive tract, or the kind of exposure that would occur in nature, and the material used for the exposure was obtained from actual cases of infectious abortion. Of the eleven cows treated with live organisms before concep- tion, 10 calved normally in all respects and 1 aborted. 560 E. C. SCHROEDER Of the 4 given dead-organism treatment after becoming preg- nant, 2 calved normally and 2 aborted. Of the 8 controls, 7 aborted. The cattle of the first group, those which received treatment before they were served by a bull, were kept under further observation until they had produced an additional 15 calves. They suffered no breeding troubles, such as retained placentas, sterility, ete. The number of animals in the second group, only 4, is too small to permit drawing the conclusion that treatment with dead organisms in some measure protects gravid cows; but the difference of 1 abortion among 11 cattle and 1 abortion among 8 can leave little doubt that a serviceable degree of immunity against abortions can be engendered by subcutaneous injections, prior to conception, of living abortion bacilli. A SEARCH FOR OTHER CAUSES OF ABORTION AMONG CATTLE A search for other specific causes of abortions among cattle has not been neglected, and Bureau investigators could relate at great length stories similar to those which other investigators have told about microorganisms isolated from the products of abortions and the uteruses of cows that have aborted. Bacilli of various kinds, different types of micrococci, and spirilla or vibrio have been found repeatedly ; but when their pathogenicity has been tested in accordance with widely recognized and ac- cepted and required bacteriological standards, not one shred of evidence has been obtained to prove them true etiological factors of bovine abortions. What réle such microorganisms may have as causes of the sequele of infectious abortions, and of other, possibly independent, abnormal processes in the reproductive organs, is far from clear and merits careful study. Bovine Inrectious ABORTION AMONG SWINE At times the Bang bacillus attacks sows and causes abortions among them. Attention has been given to this matter, but as yet little progress has been made. The Bureau’s work indicates that swine are strongly resistant against ordinary strains of the abortion bacillus, but is not conclusive on the subject. So far we have been able to procure only two strains of the bacillus isolated from outbreaks of abortion among swine, and these strains, though in other respects like those obtained from eattle, are not wholly like them in the lesions they cause in guinea- INVESTIGATIONS ON Bovine INFECTIOUS ABORTION 561 pigs. One of the strains causes lesions much grosser in character, and the other has the peculiarity of causing orbital tumors in a large proportion of guinea-pigs injected with it, which lead to a crowding forward and an extreme protrusion and the gradual destruction of the eye. While it is remarkable that both strains of the bacillus derived from swine should vary more from the general type obtained from cattle than strains from cattle vary from one another, we must remember that it is only two strains we have studied. One of the strains caused abortions in both a sow and a cow, and in this case the sow was exposed through ingestion, and the organism was afterwards recovered from her uterus and the aborted fetuses. With serological tests both strains act precisely like ordinary abortion bacilli. It will be apparent from what I have said that much work on infectious abortion has been done and that valuable results have been obtained, and it will also be apparent that much remains to be done. The work is peculiarly of a kind that requires time, diligent application, long-sustained interest and considerable expenditures of money. I hope that the outline presented will convinee you that what has been accomplished justifies that the work should be continued and liberally financed. BUSINESS TAKES GOVERNMENT PERSONNEL An editorial in the Washington Post, based upon the Civil Service report, says: ‘‘Business has a keen scent, and it tracks down its man and lures him to its own service just at the time when the display of his abilities has shown that he is about to become of greatest value to the Government. The drift of personnel from an official to a business career is at some periods stronger and more noticeable than at others, but the outlet is generally wide open and is never closed up. It is unneces- sary to point out how undesirable, from a public point of view, such a leakage is. If the Government-were a wise and farseeing employer, it would hold out such inducements to those who enter its service that the tendency to slip away from it would be searcely, if at all, existent and the exodus of its best and brain- lest officers would be stopped or greatly checked. The annual report of the Civil Service Commission, just published, em- phasizes the danger which threatens the public service because of this continuous drain on its human resources.”’ INVESTIGATIONS ON THE USE OF VACCINES MADE FROM BACILLUS OVISEPTICUS + By I. E. Newsom and Fioyp Cross Colorado Experimental Station, Fort Collins, Colorado THE SKEPTICISM regarding the value of commercially prepared vaccines against hemorrhagic septicemia, which has been aroused by the publication of Nebraska Research Bulle- tins Nos. 17 and 18 (1), has led practitioners and investigators to the point of inquiring, not only whether there is value in commercially prepared vaccines for these diseases, but whether the whole practice of immunization against them is on a secure basis. Judging from the comments which have appeared in recent publications, there are those who are not only doubtful of the value of commercially prepared vaccines, but are even questioning the posssibility of producing an efficient vaccine by present methods. It seemed to us at this time that any addi- tion to our knowledge which is based upon controlled experi- ments might be received with interest even if of no permanent value. Therefore we have collected some experiments done by us during the past four years and merely present them for your consideration, realizing that they are somewhat incom- plete and are too few for final conclusions. Ever since the classical work of Pasteur (2), in which he seemed to show that an efficient vaccine might be produced against chicken cholera by allowing the cultures to become avirulent and then using them alive, much interest has been exhibited in an attempt to produce immunizing agents against all of the hemorrhagic septicemias. Kitt (3), following Pasteur, came to the conclusion that the Pasteur method was not only inefficient but dangerous, as there was always a possibility of transmitting the disease. In this country possibly the most painstaking work has been done by Hadley (4), who found that strains of chicken cholera organisms failed in their capacity to stimulate the production of immunizing substances in rabbits and fowls. His earlier 1 Presented at the fifty-eighth annual meeting of the American Veterinary Medical Association, Denver, Colo., September 5-9, 1921. 562 VACCINES FROM BACILLUS OVISEPTICUS 563 work seemed to indicate that no value might be expected from the use of an organism killed by heating at 63° C. for 20 minutes, but that one of his strains, which was avirulent, could be used alive on rabbits, and did give a considerable degree of protection. In a later bulletin he made the discovery that a considerable immunizing value could be had even by using dead organisms. Since our work has dealt entirely with Bacillus ovisepticus, we prefer to discuss the work done by other investigators with this organism, although the literature in the whole field of hemorrhagic septicemia immunization is very inviting. In 1898 Lignieres (5), after having investigated the disease known in Argentina as lombriz, developed a polyvalent vaccine made by growing the organisms at higher temperatures than normal, thereby reducing their virulence. With this he claimed considerable success. Later, in 1910, Miessner and Schern (6), working in Ger- many, tried the method of Lignieres, but with rather unsatisfac- tory results. They then produced a bacterial extract by grinding the organisms and claimed from this procedure a considerable success. They were also able to obtain some protection by the use of an antiserum. A survey of the literature does not show that any one of these procedures came into common use in the countries mentioned. ‘Since the use of a killed organism had become common in this country for protection against hemorrhagic septicemia in cattle and swine, it was only to be expected that the same type of immunizing substance should be tried on sheep. Our work, therefore, has dealt with the value of the killed organism first, and finally of a live culture. EXPERIMENTS ON RABBITS Experiment No. 1 Our first experiment was conducted with rabbits. The culture used in this experiment was our No. 23. The vaccine was pro- duced by growing the organisms in bouillon for 48 hours, then heating in a water bath at 60° C. for 30 minutes, after which 14 of 1 per cent of phenol was added. Vaccines grown in this way usually contained between 7 and 10 billion organisms per cubic centimeter. The determination of the minimum lethal dose was made according to Table 1, 564 I. E. NEwsom AND FLOYD Cross TABLE 1.—To DETERMINE THE MINIMUM LETHAL DosrE For Rasstits. Date Number Weight (Grams) Amount (C. C.) Result May 2, 1918 1 550 0.25 Deat do. 2 550 Ail do. do. 3 550 .02 do. do. 4 550 O01 do May 3, 1918 5 825 ‘01 do do. 6 716 002 do do. v4 685 .001 do do. 8 590 .0001 do May 7, 1918 9 570 0001 do. do. 10 830 .00001 do. do. 11 685 000001 do. do. 12 885 .0000001 Lived Five rabbits were then taken and each given 0.5 ¢.c. of the vaccine. Eleven days later these rabbits were given amounts varying from one thousandth to one ten millionth of the culture. Five rabbits were given the same amounts of the culture and were used as controls. All rabbits died. At a slightly later time five rabbits were again given 0.5 ¢.c. of the vaccine and 17 days later all were given one millionth of 1 ¢c. of the culture. Only one control was used, which was given the same amount as the others. The result was that all of the vaccinated rabbits died and the control lived. This experiment indicated that the vaccine produced by us had no immunizing value in rabbits against strain No. 23. TaBLE 2.—To DETERMINE THE VALUE OF VACCINE FOR RABBITS Vaccine Culture Weight Pes? SusTeAsy ssh No. | (Grams) : Result Date Amount (C. C.) Date Amount (C. C.) 1 710 May 2, 1918 0.5 May 13, 1918 0.001 Died 2 555 do. 463 L do. .0001 do. 3 540 do. 5 do. . 00001 do. 4 885 do =o, do. .000001 do. 5 545 do. 5 do. 0000001 do. (eae Rear a Controle ar caeceacceee do. 001 do. 7 ES Oe Ar GO callicaecPi sents do. .0001 do. Jed Wcaatcue mets 1 Co al SrA a ee do. . 00001 do. D6 Pere. GOs ose ea eee. e sic do. .000001 do. LO: Mie. Sea do: 4) Sar aan se do. .0000001 do. ll 410 May 26, 1918 5 June 12, 1918 .000001 do. 12 4l do. a0) do. . 000001 do. 13 410 do. 4b) do. 000001 do. 14 410 do 5 do. 000001 do. 15 410 do. oS do. 000001 do. 16 410 Control\ se |r a eae ee do. . 000001 Lived EXPERIMENTS ON SHEEP Experiment No. 1 Our preliminary experiments having shown that not much was to be expected from rabbit inoculations, we were curious to know if any immunizing value might be had in sheep. We, there- , VACCINES FROM BACILLUS OVISEPTICUS 565 fore, selected 5 lambs weighing about 50 pounds each, and gave each of them 2 c.c. of a vaccine made as previously described. Eleven days later 3 of the vaccinated lambs were given intra- venously 5 e.c. of a 48-hour bouillon culture of strain No. 23; one was given 10 ¢.c. and one 1 cc. A control was given 5 «ec. The vaccinated sheep receiving 10 c.c. of the culture and one of those receiving 5 ¢.c. was found dead on the morning following the injection. The one receiving 1 ¢.c. died eleven days later, and another receiving 5 c.c. died on the 12th day. The control having received 5 c.c. died on the sixth day. One of the vacci- nated sheep survived. TABLE 3.—To DETERMINE VALUE OF VACCINE FOR SHEEP. (EXPERIMENT NO. 1). Vaccine Culture Lg —— Result Date Amount (C. C.) Date Amount (C. C.) 1 | June 1, 1918 2 June 12, 1918 5 Death, 1 day. 2 do. 2 do. 5 Death, 12 days. 3) do. 2 do. 5 Very sick for 2 weeks; recovered. 4 do. 2 do. 10 Death, 1 day. 5 do. 2 do. 1 Death, 11 days. 6 Montrose ace y. crore do. 5 Death, 6 days. 7 GOSMEv A set he nee. SET ASL we 0 Lived. Experiment No. 2 Having shown that a vaccine made by us was of little value, we set up this experiment in which we used two well-known commercially prepared vaccines. Three lots consisting of 3 sheep each were selected. Lot No. 1, consisting of 3 animals, was given 2 ¢.c. of a commercially prepared vaccine. Lot No. 2 was treated in the same manner with a commercially prepared vac- cine from another source. Lot No. 3, consisting of 3 animals, was used as a control. Twelve days after vaccination one animal in each lot was given 10 ¢.c. of a 48-hour bouillon culture of our strain No. 23, one was given 5 ¢.c. and one 1 «@¢., all intrave- nously. All three of the animals receiving 10 ¢.c. were found dead the following day. Of the lambs receiving 5 ¢.c., the one in lot No. 1 died three days after the injection; the one in Lot No. 2 sickened for one week but recovered; the one in Lot No. 3, being the control, died on the sixteenth day. Of the ones re- ceiving 1 ¢.c., one in Lot No. 1 sickened and always’ remained lame in the left hind leg; one in Lot No. 2 sickened and remained lame in the right hind leg for several weeks; the control sick- ened and remained lame in the left hind leg for two weeks. 566 I. E. Newsom AND F'Loyp Cross TaBLE 4.—-To DETERMINE THE VALUE OF VACCINE FOR SHEEP. (EXPERIMENT No. 2.) PEN No. 1, COMMERCIAL VACCINE No. 1. Vaccine Culture No Result Date Amount (C. C.) Date Amount (C. C.) 1 | July 26,1918 2 August 7, 1918 10 Death, 1 day. 2 do. 2 do. Death, 13 days. 3 aCe ed hy CRS EROS © Ot do. 1 Sickened and always remained lame in left hind leg. PEN No. 2, CoMMERCIAL VACCINE No. 2. 1 | July 26,1918 2 August 7, 1918 10 Death, 1 day. Be do. 2 do. 5 Sickened for a week but recovered. 3 do. 2 do. 1 Sickened and lame in right hind leg for several weeks. PEN No. 3, Controt, No VAccINE. 1 eal eae te oe 2! | Eee ae ae 8 August 7, 1918 10 Death, 1 day. DHE | | hs Atay oleate: Sues | eR Rene CEPA ANE do. D Death, 16 days. Bate | icy ear e-gen a | (RASS Seni Ce eee do. 1 Sickened; remained lame in left hind leg for 2 weeks. Although it would be presumptuous to conclude on such a small number of animals, the appearance here purely on the face of the results is that some slight protection was given by commercially prepared vaccine used in Lot No. 2. Experiment No. 3 It having seemed up to this time that little value might be expected from an organism killed by heat, in the preparation of the vaccine for this experiment, we omitted the heating and added phenol to 14 of 1 per cent. Four lambs were then vacci- nated as follows: No. 1 was given 10 ¢.c. of this vaccine, No. 2, 5 c.c., No. 3, 2 ¢.c. and No. 4, 2 ¢.e. at each of two injections, 12 days apart. Fourteen days after vaccination, each of the vacci- TABLE 5.—To DETERMINE THE VALUE OF VACCINE FOR SHEEP. (EXPERIMENT No. 3.) Vaccine Culture No. eS ee Result Date Amount (C. C.) Date Amount (C. C.) 1 Dec. 28, 1918 10 Jan. 11, 1919 10 Lived. 2 Dec.30, 1918 5 do. 10 Lived. 3 | Dee: Ne ae ' 2 do. 10 Death, 1 day. ec. 30, 1918 : 4\| Jan. 11,1919 ; 2 Feb. 13, 1919 10 Lived. 5 Control) aliss ater oe do. 10 Death, 1 day. 6 Gor eter lees do. 5 Lived. rt Cpr | emer ets ee sege aoe do. l Lived. VACCINES FROM BACILLUS OVISEPTICUS 567 nated animals was given 10 ¢.c. of our culture No. 23 intraven- ously. Three controls were selected, one given 10 c.c., one 5 c.c. and one 1 e.c. Of the vaccinated animals only No. 3, the one which had received 2 ¢.c. in a single dose, died. Of the con- trols, the one receiving 10 ¢.c. died, the other two survived. On the face of these results it would seem that some immuniz- ing value was had by the use of phenolized cultures when given in greater dosage than 2 c.c. Experiment No. 4 In this experiment it had been decided to give the injection of the virulent culture subcutaneously, since all previous work had been done intravenously. Five lambs were vaccinated with a phenolized culture as follows: No. 1 was given 2 «¢.c.; No. 2, 5c.c.; No. 3, 10 c.c.; No. 4, 2 @e. at each of two injections, and No. 5, 2 ¢.e. at each of three injections. Twenty-eight days after the first injection was administered all were given 20 c.c. sub- cutaneously. Of two controls which had been selected, three days previous to these injections, the one receiving 20 c.c. of the virulent culture died in one day and the one receiving 10 e.c. sickened but recovered. All of the vaccinated animals having survived the 20 c.c. of the culture, another control was given 20 ¢.c. on the following day, which animal sickened but sur- vived. Unfortunately this circumstance seems to invalidate any conclusion that might be drawn from this experiment. TABLE 6.—EXPERIMENT No. 4, SUBCUTANEOUS INOCULATION. Vaccine Culture eS eee Result Date Amount (C. C.) Date \Werriake (C. G.) | 1 | Jan. 18,1919 a Feb. 15, 1919 20 | Lived. 2 do. | 5 do. 20 | do. 3 sla eS: matali 10 do. 20 do. an. . : © . | 4 Jan. 25, 1919 J 2 do. 20 do. Jan. 18, ol 5 Jan. 25, 1919 2 do. | 20 do. Feb. 1, 1919)) 6 SOE ls Ie a Feb. 12, 1919 20 Death, 1 day. ci juss 5 “\ eee do. . 10 Sickened, but re- ; covered. airy = July Ds TOZO} ese thie te clo ce. Ieee: ae + af = = GO Oy ares carl oie ore ree | retatell Several eee + + - - It will be seen from Table 8 that two lambs were selected and given increasing doses of a phenolized culture of Bacillus ovi- VACCINES FROM BACILLUS OVISEPTICUS 569 septicus subcutaneously until 20 ¢.c. had been administered in this way. This procedure brought up the agglutinin content of No. 1 from 0 to 100. The agglutinin content of No. 2 stood at 1-100 on first test and was not increased. Intravenous inocu- lations o1 bouillon cultures were then started, and this was continued until 15 ¢.c. were given. The lethal dose of this cul- ture was something less than 10 ¢.c., and while the animals with- stood this dose, and the 15 e.c. dose also, yet they were made ill by both inoculations. Following the 15 c.ec. dose, the agglutinin content increased from 1 to 250, but never increased beyond this. It appeared from this experiment that it was possible to im- munize sheep against lethal doses of Bacillus ovisepticus. Experiment No. 7 This experiment was arranged to determine whether vacci- nation with the live organism was better than by the use of a phenolized culture. The whole experiment was checked by means of the agglutination test. Three animals were given 2, 5 and 10 ¢.c. of a phenolized culture subcutaneously. Three others were given the same amount of a 48-hour bouillon culture sub- cutaneously. Three were used as controls and remained un- vaccinated. Twenty-three days after the administration of the phenolized organism, and 21 days after the administration of the live culture, the vaccinated animals and one control were given 10 ¢.c. of a virulent organism intravenously. Two of the controls had previously been given 7 c¢.c. of the same kind of culture. The 10 ¢.c. and one of the 7 ¢.c. controls died; the other survived. Of those vaccinated with the phenolized organism, the one receiving 2 ¢.c. became ill but survived, the other two died. Of those receiving the live organism as vaccine, all survived without showing any signs of illness. The aggultination test did not reveal the presence of any antibodies in the blood of any of the animals at the beginning of the experiment. Two days after the introduction of 10 e.e. of the phenolized culture, agglutination was positive in 1 to 10. This agglutinating ability was quite transient, as it did not ap- pear again until 14 days after the vaccine had been administered. As measured by the agglutination test, the other two of this group showed no protection, although it will be remembered that No. 1, the one receiving the least amount of vaccine, sur- vived the virulent culture. 570 I. E. Newsom Aanp FLoyp Cross On the day following the introduction of the bouillon culture used as vaccine, all three animals showed the presence of ag- glutinins in the blood. This gradually increased in intensity until the time of the injection of the virulent culture, when the one receiving 2 ¢.c. showed agglutination at 1 to 25, 5 @e. showed 1 to 50, and 10 ¢.c. showed 1 to 100. It would appear from this experiment that the live organism gives greater immunity as vaccine than does that to which phenol has been added. TABLE 9.—EXPERIMENT No. 7. Agglutination No. Date see Remarks 1:10 2550) 1:100 July 2, 1920 oO. CONAMP whe a ° | 2 c. c. vaccine subcutaneously. 5 ce. c. vaccine subcutaneously. 10 c. c. vaccine subcutaneously. July 8, 1920 ae tet Te ci YTS TS ey Pea Ee] CRONAVBR Whe Qa ° | | als wNre ° July 10, 1920 do. do. do. Pratl [eles 2 c. c. 48-hour bouillon culture sub- cutaneously. ~ 5 c. c. 48-hour bouillon culture sub- cutaneously. 10 c. ¢. 48-hour bouillon culture sub- cutaneously. Con HA wo PwWNe Q ° July 11, 1920 oO. Temperature 105.8 Temperature 106 Temperature 105.2 reer tt ee eel AP ALES SI Testhe EE Te Te ed et ses Il Mell ete Temperature 105 July 12, 1920 oO. TL TPP SIT LL TPT SUPT Ea Lae Sn ees a ° riteer | +H VACCINES FROM BACILLUS OVISEPTICUS TABLE 9.—EXPERIMENT No. 7—CONTINUED. Agglutination CONAN BR wWhe a io) Tea etesteet= adil] PE et reall Me Leal a) PPE TE Me LS Ue tt PE aE ab TI | | | | July 14, 1920 1 00 AIO) Oe Ro a i) Te isteote=te elit Pe UE Tt LE TBS TET HU est ties Tea ee — July 15, 1920 OA CV COR a i) Te estes tanta ea Ostet deal UT TT ea] WP te We —— | | SS July 16, 1920 OOO ACE CO DD a ° Wealth | Here an AP UST TE TE LE | ULES Ts oO. COUIH ewe Qa ° TEU ieteate rea leattal WW Mega eT PLIbII tit eC Theat ee et eT _————— | | Se July 18, 1920 oO. CONAN R whe Qa ° We Stearate eel resalealh ia Cherri Oo. TS stesteete alee) Pel see CLL SHY eS Telia ete lene| CBNHAUTB Whe a ° 9) 572 I. E. Newsom AND FLoyp Cross TABLE 9.—EXPERIMENT No. 7?—CoNTINUED. Agglutination No. Date Ne ee Remarks EO 2551 150 E00 __—_—— | ————————_ | | EE EE 1 | July 20,1920 | — = “a = 2 do. ~ -- — —_ P 3 do. - _- ~ _ 4 do. aa _ _ - 5 do. + - _ _ 6 do. aa a + — 7 do. - _ — — 8 do. — -_ ~— _ 9 do. _ — — _ 1 July 21, 1920 _ - _ - 2 do. - - — — 3 do. —- _ _ — 4 do. a a — — 5 do. + — _ _ 6 do. als ate Ar AF re do. _ —- — _ 8 do. —- - —_ — 9 do. —- — — — 1 July 22, 1920 — ad - _- 2 do. - - —- _ A do. + a = = 4 do. “Le + = ~ 5 do. + + a - 6 do. aP ain + qP if do. — — — — 8 do. — _ — —_ 9 do. —- — _ ~ 1 July 23, 1920 _ — = = 2 do. — — —_ — 3 do. + - - — 4 do. + Sa = - 5 do. + + + _ 6 do. + =F oP ate 7 do. = — - — 8 do. _ - — - 9 do. _ - — _ 1 Unk pila CRI Pere S| eee Alls Aeon lla Ggor 10 c. c. 48-hour bouillon culture in- travenously 10 a. m, 2 (6 (05 Mie) eine nena Orc! nd 5 OIG | [a OetOn do.; 2.45 p. m., very ill. 3 GO Fo GH Save tevell Beare ots |S earereres| Mekorotere do.; 2.45 p. m., dead. 4 dows te ek. | Seer lee 10 c. c. 48-hour bouillon culture in- travenously 10 a. m. 5 (Rem Ibordcon) ecco loonbocllononoe do. 6 CO all -AApoollamoeodl lAécecdlloocous do. 7 ( ORE i nomon|lses sb lonsdo0||Soodot do. 8 Tulys2Z6s V9OZO a era, ell secret eteretemhl ercetceee 7 c. c. 48-hour bouillon culture in- travenously 10 a. m. 9 Ci fe aR PAS Peron ateesinest Iba nmcsl oS aac do. i |pAugustel 1920)| aay rscsllorereaers| eesti | setae Very ill; temperature 106.2. 2 ore Wie seule strceel| prebis iene lector 9 a. m., dead. E 4 Os cas Wheel | Re ee ll etebeces [eee Normal; eating; temperature 104.6. 5 Ree Bol spol lan ood! ne obud Normal; eating; temperature 104.8. 6 loys aml Earns lates otal acini nao akc Normal; eating; temperature 105. 7 GO Naser erallsictevenerei|febeterenscclll ote ions Nery al; unable to stand; temperature 8 Jul ¥:27,/1 920) 2| acta seal| sere os electoral seers Normal; eating; temperature 104.6. 9 Ci fe Cod Carnal incon oc oo oe Very ill; not eating; temperature 106.2. Ly |pAugust,) 219203 earer.jo <1] aotelsusialllonetersie|lereesters Looks better; eating; temperature 105. 4 (eee le SRS eral looms lnaocok Normal; temperature 104.8. 5 doz, | nceduell bem crse Netcool eee Normal; temperature 104.8 6 dots ol Sac asole mercial aon limeereere Normal; temperature 104.6. ii On Wesieereoaecqasl| eae leer 8 a. m., dead. 8 July 2851920) Wesel eee enone Normal; temperature 104.8. 9 i (emis meres) meres Imetianyes S| [Kis mon Down; unable to rise. VACCINES FROM BACILLUS OVISEPTICUS 573 TABLE 9.—EXPERIMENT No. 7—CONTINUED. ¥ ae Agglutination No. Date ee eee Remarks PTO! #225 (ft F250) |) 1-200 1 | August 3, 1920 4 do. 5 do. 6 do. 8 July 29, 1920 9 do. i Sep. 14, 1920 4 do. 5 do. 6 do. 8 do. Experiment No. 8 This experiment is practically a duplication of No. 7, with a view to determining whether the live organism is of greater value as a vaccine than the dead. TABLE 10.—EXPERIMENT No. 8. Agglutination No. Date Remarks PAO 1 E25) | E50) | 15800 eee ee eee ee ee ee ee CONAME Whe Qa ° Lili, TIL Ta a | es (ee [a eee |e eee 2 c. c. 48-hour bouillon culture sub- cutaneously. GPE 8 DE Se) ES (ae ea Dee 5 c. c. 48-hour bouillon culture sub- cutaneously. RAS | 7 joreretels.cce crore | Chine aorelficlosevae 10 c. c. 48-hour bouillon culture sub- cutaneously. INE) UG TIPE OS el eee] Cee [eee 2 c. c. vaccine subcutaneously. Git, “+ Lake Cie) Ie pec Re ES ae] (ae eae 5 c. c. vaccine subcutaneously. ere |S fe clereicics| obsess orais [inne carne 10 c. ce. vaccine subcutaneously. Ane WO HH Q ° IL Iti +++ eT eo SU 1] eT ST Laat LY ad es CAL lt a CONIA R whe Qa ° 574 I. E. Newsom anp FLoyp Cross TABLE 10.—EXPERIMENT No. 8—CONTINUED. Agglutination Z 2 Date Remarks 1:10 | 1:25 | 1:50 |} 1:100 Feb. 17, 1921 oO. OO IDC WOR a ° Pearl dsreetleaaycl| Pa A etd) SE Th eA A eT —— | |W | | | Feb. 18, 1921 CONIAVR we Qa } Worl) disetoriel WE Ty ses Th Tet LALe Leelee Wea eee veal | | Feb. 19, 1921 CONDO OO Qa ic) [steal Uistealistestaal eT VIPS ial alk ee VU TE Ue Beal —— | | | | EE Feb. 21, 1921 WOON ATR whe a 3 eal bate taal Tee eee eT ll ETS aTES| HU SLT Feb. 22, 1921 WeONAVPwWNe Q ° I-11 +++ SU SE Msi ee as te tll | Feb. 23, 1921 CON QnRwoe a ° Hie tisedisiraed De aL TE a Tea) al LE te AP A eet ey —_——— | FE | Feb. 24, 1921 [+1 -+++++H TE ase TE UN a HA Ie r WoOdIane whe Qa ° VACCINES FROM BACILLUS OVISEPTICUS 575 TABLE 10.—EXPERIMENT No. 8—CoNTINUED. Agglutination No. Date Remarks PeTON 2551501. 100 Feb. 25, 1921 9. He Me Ue eh HOTOH NS WA ect CBNAMR Whe a ° Feb. 26, 1921 do. eT IMAL te Ba] Nea AP Ah Ath ad CONIA whe Q ° Feb. 28, 1921 0. DOE Q ° Darr eaaal (eabee een Pee CONIA whe a io) WU (iN de tel) VE] AP Tall: Uebel al ——— | | | S —___—_ DSTA ele Pett Mths LP? [Ti Mette ———— | | CANA Whe Qa ° Itt VT Uh) see De aes alictal | | | Deas ee March 3, 1921 do. bb t++it+t CONAT Rh Qa ° b+ b+ eee tet Hel SE Qa [-} I+ 1++4+4+4+ eee Lt tit t ++ CANA whe Qa ° 576 Il. E. Newsom Aanp FLoyp Cross TABLE 10.—EXPERIMENT No. 8—CONTINUED. Agglutination No. Date Remarks 1:10 | 1:25 | 1:50 | 1:100 ——_— | | | | | I) b++ +++ 2 ° Ipt++++4++ PPT tee eb SL tka there'll eed fr Se | |] | | March 10, 1921 Oo. Ey |++++++++ Lb b+ +++ PL i+l i++ bhatt t+i Wiinven 1p OPAlle eel aneccllaudleso|loan cs 2.40 p.m., 20 c.c. 48-hour bouillon cul- ture intravenously. E 7 oO. Ee new An iyAl Sa son Bila ei bl [piayase 2.40 p. m., 10 c. c. 48-hour bouillon culture intravenously. (Pee bl MESES 4] Monee. | Cmoteclitacsce 2.40 p. m., 15 c. c. 48-hour bouillon culture intravenously. don Phileas etalon | eho 2.40 p. m., 20 c. c. 48-hour bouillon culture intravenously Oo OO NAVMPwOh Qa ° Qa ° a $e VACCINES FROM BACILLUS OVISEPTICUS 577 TABLE 10.—EXPERIMENT No, 8—CONTINUED. Agglutination % Date Remarks "9 TT Oe t-25) 1507 | 1100 MVinrenli pel Oo | Shoe Items June, 1920 June, 1921 UAPT OT 6 LA OR Gene | Os OCR Een ee ee ee See 2,188,012 2,139,155 Re oo. cs wei oaecescccwwas 7,157 4.279 AIT 5 Boca: Gale HEP RI aia Se RSS o ees Aa aig mae 1-304 1-499 622 W. J. EMBREE TABLE 3.—Ratio oF Loss IN THREE SPECIES OF Foop ANIMALS AT ELEVEN PRINCIPAL WESTERN MARKETS Ratio, Dead to Received Species June, 1920 | June, 1921 Catiles a8 - socn trot oteae cc sa ee ee 1 to 2,600 1 to 2,847 Gattlotand! calvestee oes ce oe eae, : Soe nae aoe oe 1 to 1,100 1t01,175 SLE inet Cee oS ene Ee ae are RMR os habe ine ene 1101,600 1 to 1,949 [3 Cy hon eM phat I OSU Be Melina ee Se RAR BS Ss i Ai lto 300 lto 500 there in this manner. We have nothing to do with these ship- ments and keep no record over an extended period of time. During eleven days for which a record was kept the ratio of dead in truck shipments was one dead to every 614, compared to 1 to 853 in railroad shipments for practically the same period, showing that hogs will die in transportation when they never see a stock car. This has also been verified by investiga- tion at other markets. We now come to a subject which is rather bothersome to the railroads, and that is the matter of ‘‘overs’’ and ‘‘shorts’’ at markets. There is often a possibility of hogs becoming mixed when being unloaded from railroad cars. Sometimes the chute gate does not close tight against the side of the car, leaving a space of 16 to 24 inches through which small animals may escape. We strongly recommend to stockyard companies that they place an auxiliary slide gate in the chute that may be closed up tight against the side of the car. This need not be done at once, but in repairing the old gates as they wear out this slide could be put in. The interlocking toe-board is very valuable and necessary ap- paratus, especially for the handling of horses and mules. The greatest cause of loss in cattle shipments is thin condition of flesh. A much larger per cent of thin cattle die in transit than any others. Some cattle of this class are shown in figure 3. These cattle were a Texas shipment in the fall of 1917. An explanation of the digestive apparatus of the ox will show why cattle shrink so greatly in transit. Two-thirds of the ab- dominal cayity is taken up with the first stomach, called the rumen or paunch. It is possible for this organ in a 1,000-pound animal to contain 180 to 265 pounds of undigested food. When the animal is kept in transit for some time this food will be digested and evacuated, and of course the amount that is di- SSS PREVENTION OF LIVESTOCK SHIPPING LOSSES 623 gested from this paunch decreases the animal’s gross weighi to that extent without the body tissues being drawn upon to sustain life. It follows that shrinkage in transit does not involve meat loss unless the animals are kept off of feed for many hours. Meat is what is being produced. Cuts and bruises are other causes of loss of meat. Cattle are sometimes bruised and cut by the horns of other animals in the same car. Hogs are bruised by the use of whips, quirts and clubs in driving them. Great effort is being made to have all such implements of torture discarded by livestock handlers at our large market centers. The prod pole with a steel point surely belongs to the dark ages and should be discarded by all means, as every prick from a prod pole means a loss of meat, for the reason that injured portions must be removed and very often cuts of meat are spoiled thereby. Fig. 3—Thin cattle, a class among which losses In shipment are heavy, The modern method of driving hogs is by the use of the slap- jack. Hogs that are handled in that way come from the scraping and cleaning in the packing house in good condition and there is no loss or spoiled cuts of meat on account of bruises or injuries. Mr. R. L. Carter, of Swift & Company, has compiled the fol- lowing statistical information in regard to loss from bruised meat : Beef Loss on Account of Bruises, Week Ending March 20, 1920 Cattle killed, 25 markets......... beads . 46,789 Cattle bruised (26 per cent)... bias 5 O28! . 12,059 Meat removed on account of bruises . 15,933 pounds 624 W. J. EMBREE Hog Loss on Account of Bruises During the Year 1919 Hogs killed, 538 markets......0.........0.. ce eR AE 30,512,000 Estimated loss of meat per Ogee ssnccssssescsenees One-half pound Total loss.of meat, ‘h3,markets...2..4 2 does 1) 15,256,000 pounds Average live weight, CHica gna. iscscenecsccsesneecessrnnecceome 190 pounds Average live? enst, (Chieago: 2 ose. Alou eee ee $17.85 Average dressed COSt, CHICA 20. eeessssocsseessnneseeesnseseseenn $23.00 Total loss on account Of DrUiSeS oo cci ice ccccesccctecmensccce $3,508,880.00 Loss of meat equal to 80,295 hogs, or about 820 cars. Hog Loss on Account of Bruises, Week Ending March 30, 1920 Hogs \kilted, 37 markets.c: ¢60)) od ees ee ee 229,606 Total loss of meat-Per Nop .5.0 ie ee One-half pound Total loss Of meat, oT markets ee 114,803 pounds Average live weight, CHica onc iceceesecsseccsssscccesesssesseemencecesnnecee 238 pounds Average live cost, Chieaso> =o = |) O6 ee $15 Average dressed cost, Ghicage2 0-2 eee $20 Totakloss. on. account) of bruises 2 es Fo $23,860 Loss of meat equal to 501 hogs, or about 5 cars. COOPERATION BETWEEN VETERINARY AND HUMAN MEDICINE. The London correspondent of the Jowrnal of the American Medical Association writes as follows: ‘The value of cooperation between veterinary and human medicine has often been pointed out, and has recently been in- sisted on by Sir Clifford Allbutt, but has never been carried out. Mr. Scott, president of the Mid-West and South Wales Veteri- nary Medical Association, has drawn up the following proposals: (1) A veterinary officer should be attached to the medical de- partment of the ministry of health. (2) A whole-time veteri- nary officer should be appointed in all large cities to work in conjunction with, but not subordiate to, the health officer. (3) In provincial towns a part-time veterinary officer should be ap- pointed to work with the health officer on the same basis. (4) Research work in comparative medicine and pathology should be carried out in research institutes and universities open to re- searchers following human or comparative medicine. (5) The older universities should found chairs of comparative pathology. (6) The universities should grant degrees in veterinary science, and postgraduate courses should be arranged. (7) A list of diseases communicable from animals to man should be fully lrawn up by physicians and veterinarians, and a closer study of these instituted, particularly prophylactic. (8). The. medical and veterinary societies should hold joint meetings to exchange yiews on matters of mutual scientific import.”’ DUNCAN McEACHRAN AND THE McGILL FACULTY OF COMPARATIVE MEDICINE! By VeRANUS ALVA Moore Dean, New York State Veterinary College at Cornell University, Ithaca, New York IT IS IMPORTANT, among the exercises commemorating the achievements of a hundred years in a great university, that the influence exerted by one of its faculties, that no longer fune- tions as a part of the institution, should not be forgotten. It often happens that a worthy undertaking is brought to an untimely end for want of vis- ion or lack of support. Such was the case with the MeGill faculty of comparative medi- cine and veterinary science. It was organized in advance of the time when the people recognized the economie and sanitary significance of ani- mal diseases, and also before there was much _ definite knowledge concerning them. Under such circumstances, in an undeveloped country as this was at that time, it is not surprising that the true pur- pose of veterinary education Duncan McEachran, LL. D., was neither appreciate¢ or een. as neither appreciated n understood. Dr. McEachran opened the Montreal Veterinary College in 1866 and directed its activities so long as his individual efforts and resources sufficed. When conditions changed educationally and adequate support failed, he had the courage to close its doors. A kind Providence has allotted him fourscore years in which to witness the fulfillment of his early prophesies and the 1 An address given at a complimentary dinner to Dr. Duncan McEachran at the Ritz-Carleton Hotel, Montreal, Canada, October 18, 1921. 625 626 VeraANus Atva Moore adoption of many of his ideas concerning veterinary education. It is a gracious act, at this centennial of the university to which he has been so loyal, for his former students to pay him this tri- bute in appreciation of his many and valuable services to them and for their cause. There could be no compliment more ex- pressive of the high esteem with which he is held than this gath- ering of distinguished men from many States and Provinces. Likewise there could be no testimonial more acceptable to him than to be assured in this manner that his labors were appre- ciated and that his graduates have played a worthy part in the development of the veterinary profession on the American con- tinent. My duty, as I see it, is not to dwell on the personal achieve ments of Dr. McEachran, nor to picture the trials and discour- agements that he met and overcame, nor to detail the attainments of his former students. All these are matters of record that the future historian will arrange in proper sequence for the coming generations. The task I should like to perform is to bring into bold relief the foundation ideas inaugurated by him and the results of the influence of his work, and that of the graduates of the institution he founded, on veterinary education of today; yea, of tomorrow as well. In order to appreciate the value of McEachran’s services to his profession and to understand why the school he founded ex- erted the lasting influence for good that it has, we must consider them in connection with the time and the environment in which they operated. Every cause that has sueceeded in making the world better for human existence has had a long and trying struggle before the benefits sought have been attained. Like- wise, the conception of veterinary medicine and the evolution of a profession to apply the principles of science for the relief of dumb creation and profit to animal industry has had a history that must be reckoned with in understanding the part taken by any man or institution that has contributed to its success. In the development of veterinary medicine the same forces have operated that determined the growth of human medicine. The theories of the early writers to explain the nature of disease sradually became differentiated into a variety of doctrines, each having its advocates. In turn, these stimulated other thinkers to bring forth still more explanations. Individualism domi- Duncan McEAcHRAN AND McGiuu Facuury 627 nated all there was in medicine. The success and subsequent failure of this system is illu/trated in the work of Apsyrtus of Bithynia, the veterinarian of the Roman Army, who, in the third century, controlled glanders, recognized the sanitary significance of isolation and mechanical support in the treatment of frac- tures. As the reason for his successes was not explained, the advances he inaugurated disappeared with his death. A succes- sion of distinguished leaders in the art of healing, each intro- ducing new subject matter with a different explanation as to the cause of disease, tended to confuse and ultimately led to the founding, in the ninth century, of the University at Salerno, which was not only the first university but also the first medical school. Its purpose was to ascertain the truth in the theories set forth by individuals, to correlate all the facts into working hypotheses for their application and to provide methods for teaching the new subjects that were being introduced. It is interesting to observe that nearly a thousand years later radical changes were called for in the educational system of our country to meet the requirements for its development. This found expression in the law establishing the Land Grant Col- leges in the United States. A comparison of the arguments in favor of this law used by Senator Morrill with the reasons ad- vanced for organizing the first university will show a most striking similarity between them. -The purpose of each was to find a means by which the truths and principles in the existing knowledge could be determined and made available for the benefit of the people. It is from the Land Grant Colleges that most of the permanent veterinary schools in the United States have arisen. The others are associated, as in Canada, with large universities. The achievements of the veterinarians in the earlier centuries availed but little in the revival of veterinary science after the Dark Ages. Even the prestige of the earlier schools seemed to have been lost and it was found necessary to begin again. There was the same need that there was in human medicine for trained men to care for the sick and injured. The situation ap- pealed to one Claude Bourlegat, who organized, in 1761, the Veterinary College in Lyons, France. Then followed, in rapid succession, the establishment of numerous schools in many countries for the purpose of conserving the animals that were 628 VerANUS ALVA MOORE being injured in war and lost from disease. While some prog- ress was made in the improvement of methods for the appli- cation of existing knowledge, modern medicine, human and comparative, actually began with the work of Davaine, Virchow, Pasteur, Lister and a host of others working simultaneously and following them in the field cf natural sciences. These men ex- posed the fallacies of the former conceptions and traditions of disease and established in their place the fundamental principle that diseases are the natural consequences of natural causes and that prevention is infinitely more important than treatment. This conception, which had been foretold with the conviction of revelation by many earlier workers, had its birth in the experi- mental method of study introduced by Pasteur and his ¢o- workers. The period of transition from the empiricism of the ancients to the acceptance of the idea that diseases should be studied and treated as objects of natural history was destined to be one of bitter controversy. It lasted for nearly a generation, and for all time it will require earnest, intelligent endeavor to main- tain belief in the modern conception of the nature of disease and the new function it brought to the medical professions. It was during the time that the specific nature of infectious diseases was being demonstrated, and the natural causes for the others were being determined, that veterinary education began on the American Continent. Prior to this a few unsuccessful efforts to establish veterinary colleges in the United States had been made. Practice was restricted to a few graduates from European schools and a much larger number of men of no prep- aration or technical training who believed they could in some way drive disease away. The rapid growth of animal industry, the increase in losses from disease due to the long oceupaney of the soil, and the introduction of plagues through the indis- criminate importation of animals were signals not to be mis- taken for a more efficient veterinary service. At this time, when the tenets of the profession were being shaken to their very foundation, when old theories were being abandoned and a new knowledge was springing up, five young, ambitious veterinarians, trained in the best schools of Europe, came to America and undertook, each in his own way, to develop veterinary education in the land of his adoption. - DuncAN McEAacuran AND McGinu Facuity §29 They were Alexander Liautard, who came from France to prac- tice in New York City in 1859, but soon assumed the respon- sibilities of conducting the New York College of Veterinary Surgeons; Andrew Smith, who came from Scotland to Toronto in 1861 and started the Ontario Veterinary College; Duncan McEachran, also from Scotland, who came to Woodstock, Ontario, in 1862, to practice his profession; James Law, a grad- uate of the Edinburgh Veterinary College, who was chosen pro- fessor of veterinary medicine and surgery in Cornell Univer- sity in 1868; and Henry J. Detmers, who came to America from Germany the same year and entered educational work, first at the University of Illinois and later at the Ohio State University. These men, their students and the graduates of the institutions they founded, have determined the veterinary pol- icies in our two countries. It would be interesting and helpful to portray the success of each of these truly great men in carrying out his ideas relative to veterinary education and service. They and their students established not only the veterinary schools but also Federal and State organizations for protecting livestock from diseases that arise from within and scourges that otherwise would come from without. The progress they made was won by battling against ignorance, the objections to new theories for the pre- vention, control and treatment of animal diseases and the vig- orous opposition to higher educational requirements for veter- inary students. The outcome of the generation in which they lived has been to give these countries veterinary colleges with preliminary educational requirements and length of course that compare favorably with those of human medicine. The growth of veterinary medicine in Canada and the United States has been so rapid, and those interested in it have become so numerous, that the work of these five great leaders will soon be overlooked by veterinary students: because of the enormous volume of new subject matter which science is revealing and which is being organized to aid in making an efficient, working profession. It seems proper that we should reflect on the won- derful inheritance they left us in perseverance, scholarship and character. Fortunately, all of these men were blessed with good health and long life. However, of the five, ‘‘the reel of four has been wound”’ and the shuttles lie empty, but for the 630 VERANUS ALVA MOooRE fifth, our guest of honor, there are remaining other, and we hope many, threads to be added to the woof of his already long and useful life. His strenuous efforts for the benefit of his pro- fession, especially in raising its standards educationally, are likely to be underestimated by those who pass judgment on the influence of a college that did work of a pioneer nature and which thrived for a time and then disappeared. In this con- nection it is well to remember the principle, underlying all ereat achievements, that ‘‘progress is made sometimes by re- treats as well as by advances.’’ He is a wise general who knows when to halt his troops in order that that which has been gained may not be lost. In 1866 Dr. MecEachran severed his relations at Woodstock and came to Montreal. At the suggestion of Sir William Daw- son, Principal of the McGill University, Dr. George Campbell, Dean of the Medical Faculty, and Major Campbell, President of the Board of Agriculture of Lower Canada, he inaugurated a course of lectures in connection with the Medical Faculty of the University. The veterinary students were admitted with the medical students to all classes embraced in the veterinary eur- riculum. McEachran was appointed director and lecturer on veterinary medicine and surgery. From the inception of the school he set himself to the task of raising the educational standards of his profession. He was the life and soul of the school until he had taught those who became co-workers in this labor of love. At this time, when the British and American veterinary schools required no matriculation tests, MeEachran inaugurated an entrance examination and insisted on a course of three sessions of six months each instead of two that were required in other veterinary schools. In human medicine two years only were required. Several distinguished men in the medical faculty cooperated in this work. Crask in chemistry, Fraser in physiology and pathology and Dawson in botany were among those who, in the early days, taught and inspired vet- erinary students. Thus began the third veterinary college on the continent and the second in Canada. It was only those possessed of a prophetic vision who could understand the necessity for the exacting specifications of Mce- Eachran in organizing this new veterinary school. He under- stood then—as we all do now—that preliminary educational DuncAN McEaAcHRAN AND McGint Facuutry 631 . training is essential on the part of the students to enable them to understand the more difficult technical subjects in a pro- fessional course. Again, he recognized that three years was a minimum time in which students could be disciplined in the subjects of the curriculum. It was this call to higher ideals in the profession, which foretold more efficient service, that ap- pealed to the manhood and the intelligence of many young men who, I believe, would not have studied veterinary medicine without it. Measured by present criteria, the requirements de- manded were small; but judged by the standards of fifty-five years ago, they were outstanding and conspicuous for the long step they took in advance. The prerequisites advocated and won by Law and Detmers many years later were no more pro- gressive, because of the general educational attainments of the time, than were those insisted upon by McEKachran in 1866. At first the Montreal Veterinary College occupied rooms with the McGill Medical Faculty. Later McEachran, at his own ex- pense, built the college on Union Avenue. In 1889, on the ree- ommendation of the Faculty of Medicine, the Governors of Me- Gill University created a Faculty of Comparative Medicine and Veterinary Science, and appointed him Dean and Professor of Veterinary Medicine and Surgery. Although the Montreal Veterinary College, and later the Fac- ulty of Comparative Medicine and Veterinary Science, was af- filiated with McGill University, it did not receive financial sup- port from that source. The Government finally gave the col- lege a grant of $1,800 annually, but beyond that it was sup- ported by the fees of the students and the generosity of its Dean. The University, however, continued to give instruction to the veterinary students in such subjects as their curriculum ealled for that were being taught by the Medical Faculty. It was the inspiration engendered by such men as Osler, Adami, Mills and Wyatt Johnson, as well as by those who preceded them, that extended the reputation of the school. Men came to it not only from Canada, but from beyond her boundaries. They came to it, or went from it, to 26 States and Territories in the Union, as well as to Africa, Australia, Cuba, England, Hawaii, Ireland and the Philippine Islands. Of the approximately 312 graduates, 148 came from or went to the United States. The high reputation of the McGill Veterinary College in 632 VeRANUS ALVA Moore the United States was established through the excellent work of her graduates. They were leaders, whether among practi- . tioners, those doing State livestock sanitary work, or teaching. In almost every group of men interested in these subjects, we find a McGill man. Such men as Pierce, Hinckley, the Miller brothers, Mulvey, McCracken and many others, imparted to their respective communities not only a conception of service but also one of dignity in the practice of veterinary medicine. These men have been very influential in stimulating educated young men to enter the profession. Lamb in Colorado carries the authority and influence he does because of his excellent work in State veterinary service. Higgins was a leader in laboratory work, and Blair is giving the world a great wealth of new knowl- edge on the intelligence of wild animals and their care in eap- tivity. Ramsay is doing a work in the eradication of disease that will make him famous for all time. Among the teachers and research workers in veterinary medicine in the United States, Willams stands in the front rank. His results in the development of important surgical operations, especially the one for roaring, known by his name, has given him an international reputation as a surgeon. He was among the first in the study of the diseases of breeding cattle. His findings have done much to point the way to a successful method for the treatment and prevention of abortion and sterility in cattle. His treatise on this subject, which is just off the press, will be welcomed by the profession. Further, he is the author of the best text-book on veterinary obstetrics and also of the first series of exercises for laboratory instruction in practical veterinary surgery. I know of no teacher in this or other fields of veterinary science who has done more to simulate research and to bring the profession into its own than this distinguished alumnus of your Alma Mater. In Canada many responsible veterinary positions have been filled by McGill men. The creation of the sciences, always a slow process, had reached a period of intensification. The last fifty years had been ‘‘the most prodigious period of change through which the world has ever passed.’’ More progress had been made during this time than in the previous hundred centuries to solve the prob- lem of human control of nature’s forces. It was not strange, therefore, that the Dean, recognizing the advances that had we DuncaN McEAcHrRAN AND McGitu Facuuty 633 been made in the physical and biological sciences and the neces- © sity for well-equipped laboratories and better facilities for teach- ing clinical medicine and for research, decided that if these could not be provided it would be better for the school to dis- continue. He had a fitting realization of his duty to the stu- dents, to the alumni, to the profession generally and to the live- stock industry of his country. He had spent lavishly of his personal funds, but with the rapid progress of the profession they were insufficient, and, failing in his efforts to obtain finan- cial support for his faculty, he tendered his resignation in 1903 and the school was closed. The Board of Governors of McGill University appointed him professor emeritus and in 1909 hon- ored him with the degree of Doctor of Laws. Dr. McEachran is to be congratulated for his act which led to the closing of the school, much as at the time it embarrassed him, the University and the alumni. He believed the school should continue along the lines he had formulated to provide an adequate veterinary service to safeguard the animal hus- bandry of his country. He feared disaster to the industry if properly trained veterinarians were not available. It is a com- pliment to him that he saved the good name of the institution. With the inevitable development of the livestock industry of Canada, it is not unlikely that the University may be able to re- store its Faculty of Comparative Medicine and to continue the important work so nobly started in 1866. MecEachran’s interests in his profession were not limited to the school he founded. He assisted many undertakings where his technical knowledge was needed. He early became asso- ciated with the Government in connection with livestock sani- tary work, in which he was most successful. It is difficult for us to appreciate the trials that beset those seeking the enact- ment of the first laws giving authority for the organization of livestock sanitary work. The people were living in a vast and beautiful country, abounding in animals and free from the plagues that staggered the Old World. The expanse of ocean between the old and the new countries seemed to give protec- tion against the scourges abroad. However, McEachran saw that the increasing importation of purebred animals for breed- ing purposes would introduce, sooner or later, serious maladies if the country was not protected against them. In a letter to 634 VERANUS ALVA MOORE the Minister of Agriculture, under date of September 28, 1875, published in The Farming World, he called attention to the fact that eattle plague, contagious pleuro-pneumonia, foot-and-mouth disease and sheep pox had been introduced into Great Britain by importing stock from infected districts on the Continent of Europe, and emphasized the necessity of protecting Canada against such invasions. The result of his efforts was the estab- lishment at Fort Levis of a station for detention, with the con- sent of the owners, of imported animals. The value of this meas- ure was recognized promptly and other stations were provided. In 1876 he organized ‘‘The Cattle Quarantine System of Can- ada’’ and for twenty-seven years was its executive head. It has been reported that it was the most successful and complete or- ganization of its kind. In 1879 he was sent by the Canadian Government to the United States to investigate the pleuro-pneumonia situation. His report led to important action by the Canadian Government to prevent the importation of that disease. The immunity Can- ada enjoyed from that scourge was due to regulations formu- lated on his recommendations. The arguments he advanced for a thorough quarantine are set forth very clearly in an article which he read at the thirteenth annual meeting of the United States Veterinary Medical Association and published in the first number of the American Veterinary Review (1877). I quote from the concluding paragraph. It may do us good to read what he wrote 45 years ago. ‘‘In conclusion, gentlemen, I would suggest to this Associa- tion, that you should approach the Government and point out the necessity for preventative measures being adopted and urge upon them the importance of recognizing this profession, and ceasing to appoint uneducated men to positions of responsi- bility, while you have young men edueated in science, both able and willing to fill the positions. Let our motto ever continue to be ‘Vis Unita Fortior,’ and instead of our noble science * %* * being looked upon as scarcely respectable, we must and will stand side by side with all the other liberal professions. And I certainly do think that if we can bring about the objects of this paper we will do much to deserve the lasting thanks of this great people.’’ In the same volume there is a series of articles on veterinary education and its significance in the prosperity of our animal om, DuncAN McEAcHRAN AND McGinu FAcuLty 635 industry, that both veterinary officials and teachers can still read with profit. The influence of McEachran’s work in Canada did much to aid Salmon in organizing the Bureau of Animal Industry in the United States Department of Agriculture in 1884 and in formu- lating regulations for the mutual protection of Canada and the United States in the interchange of livestock. It was through the efforts of these men that we have been safeguarded against the infectious diseases that are reported to have cost England alone more than $400,000,000 during the first quarter of the nineteenth century. In 1896 McEachran accompanied the Minister of Agriculture to Washington and Assisted in arranging an agreement by which the quarantine between the two countries was virtually abol- ished. This opened the markets for mutual advantage to the livestock trade between the two countries. In 1897 he visited for the Canadian Government many of the European countries to study the situation in each relative to the existence and methods for the control of communicable livestock maladies. His report on this inquiry is worthy of careful study. In 1899 he represented Canada at the Seventh International Congress for the discussion of international livestock trade. McEachran did much to enlighten the people on the preven- tion and control of animal diseases as well as to enforce pre- ventive measures. He issued from time to time bulletins on the diagnosis and prevention of tuberculosis, sheep scab, glanders and other communicable diseases of sanitary and economic im- portance. He was interested in meat inspection and was among the first to recognize ‘‘deficiency’’ disease among animals. He was an unquestioned leader in livestock sanitation. I am not unmindful of the criticisms that have been made on the shortcomings of the quarantine and other protective meas- ures that were instituted in those early days. Undoubtedly there were many errors and omissions. In this connection, I am won- dering if the veterinary teachers and officials of more recent times, or even of today, are meeting the professional obligations in safeguarding and bettering the animal husbandry of our two great countries with fewer mistakes and with more foresight than did the men of a half century ago. They were active in building up the efficiency of the practitioner as well as in exercising their 636 VERANUS ALVA MOORE official duties in the enforcement of sanitary regulations. The technical knowledge now available is vastly greater than that which they had to guide them, but the purpose of the profession and the necessity of preparing and protecting practitioners are the same now as they were then. As one analyzes the problems they encountered, there comes a feeling that they recognized the complexity of the situation and guarded more cautiously every integral part of the composite service necessary to give pro- tection to animal owners than is being done today. Veterinary efficiency means not only official protection against epizooties, but also a requisite number of trained practitioners to aid indi- vidual owners. It was the recognition of the value of the teacher, the practitioner and the official, and of the ethical relationship and interdependence among them, that made McKachran strong. We of today have much to learn from the work and teaching of McKachran, Law, Detmers and Salmon. The activities of McEachran were not confined to veterinary medicine. He was instrumental in the development of cattle breeding in, and the exportation of cattle from, Canada. In 1881, in association with Senator Cochrane, he purchased and was general manager of a large ranch in southern Alberta, where they maintained a herd of some 12,000 cattle and several hun- dred horses. In 1883 he severed his relations with this ranch and established, with Sir John Walrond, the Walrond Cattle Ranch, consisting of over 37,000 acres of purchased land out of a lease of 300,000 acres in the Canadian Northwest. They started with some 12,000 cattle. In 1886 he began extensive horse breeding with a stock of 250 well-selected mares. He im- ported several high-class Clydesdale and Shire stallions. This was a valuable asset to the horse industry of Canada. He was a horseman of recognized ability. He often judged at the Na- tional Horse Shows, and he was the sole judge of Thoroughbred horses at the World’s Columbian Exposition in Chicago in 1893. At the time of the Boer War he exhibited his ability to meet difficult situations. Because of the nature of the country in South Africa, the British men and horses were having diffi- culty in overcoming the hardships involved. Lord Strathcona appealed to the Doctor, thinking Canada might furnish relief. McEachran recognized at once that the Alberta cowboys resem- bled the Boers in their mode of life and work, and that no bet- Cee oc ; : , % i Duncan McEAcHRAN AND McGmu Facuity 637 ter horses could be found for the task than seasoned cow ponies. These are thoroughly trained saddle horses, accustomed to carry heavy loads. to rush up and down hills, to ford and swim streams and to find their food when turned loose. Men and horses were quickly assembled by the Doctor, who personally selected the horses. This scouting regiment was known as the ‘‘Strathcona Horse.’’ ) ie) “TO WO op Vor bra shee c eo elie « ¢e.6 este is silo a f wie! cs suele PERE EEC ALUN CELLET COL PS OE INAS SRE a Leo peoqd ‘op € ‘op ‘op ETE “WO “WO >: Me) 2G) “WO >; WO) £ O'S “A‘S'OVH | Bese ‘op ‘op ‘op ‘op ‘op ‘op top [rcs fete eles eee 1ZRe -jorjuoo ase ‘op ‘op ‘op ‘op ‘op top [ort is[o teres ee elon eee eens rivaits ‘op ‘op ‘op ‘op ‘op “op C0 “op ‘op 6OL8Ee ‘op “op ‘op “op ‘op ‘op SO ‘op ‘op SI8Ee Qe Wy op ie 0p ‘Op $0 ‘dl ‘OD S'O'H | LI8é *yosqyuoo oder) ‘Op ‘Oop ‘op ‘op ‘op SO DF Ne ak ote aed [age cee Nae a | RR na OT8E - ‘op ‘op ‘op ‘op “op “op £ ‘op ‘op CI8E Wo “TO WO WO D0) YO © 0's ‘DO -SO'H | VI8t ‘op ‘Oop ‘op ‘op ‘op ‘Oop BO) 0) A asad asa | as ee ow aa | (le ee £1ge “poryuoo o5e') ‘op ‘Oop ‘op ‘op ‘op Hoy ay Ve ‘9 op Pate val (me MRE“ aceasta ZI8e sop 2OR QY iOR co Op s 0 9 ‘Op 118 ‘op ‘op ‘op “op ‘op ‘op SO ‘op ‘op OT8E ‘op ‘Op ‘op ‘op ‘op ‘op C0 ‘dil “a ‘SO'H 608E *"jorqUoOD ase) ‘op ‘op ‘op ‘op ‘op 50) 0 | Rebbe se onl RR anit 1a Se AE 808 ‘op ‘op ‘op ‘op ‘op ‘op ¢ ‘op ‘op LOBE |e ees od “tO >: We) “MO “10 “TO “WO £ DS €S°O'H | 908E ‘op ‘op ‘op ‘op ‘op ‘op top [oicncs steps bee ee eee eee coe *JOsyUOS ater) ‘op ‘op “op ‘op ‘op LOD eee Sa OE SRS SR eae as POSE ‘op ‘op ‘op ‘op ‘op ‘op SO ‘op ‘op €ose ‘op “op ‘op ‘op ‘op “op (0) ‘op ‘op ZO8E ,OR ‘op “Op sO 492 “op s°0 ‘dl V-SO-H | 108 *joay U0 over ‘op ‘op ‘op ‘op ‘Op ‘op cn aa i a cite” in| cen lage ade OO8e ‘op ‘op ‘op ‘op ‘op ‘op £ ‘op ‘op 6628 SON | DUNO) WoO “wo “WO : ; 86LE op ‘op ‘op | Xe) 0) ‘op ‘op SZOP -Joayuoo afer ‘op ‘op ‘op ‘op ‘op op [orc tess c fects estefan eee ees POF ‘op ‘op ‘op ‘op ‘op ‘op 0 ‘op ‘op ETOP ‘op ‘op ‘op ‘op ‘op ‘op 60 ‘op ‘op ZZOP ‘op ‘op ‘op ‘op ‘op ‘op G0 ‘op ‘op TZOP [occ eee ees od “op ‘op ‘op ‘op ‘op ‘op $0 pall NY? STRIATE) SO Pas |e ence eae ee ease ne ee OSnoT PUY ‘JoajuOD oFer) ‘op ‘op ‘op ‘op ‘op op [rors tes cette eee ees GOP [oc od ‘op ‘op ‘op ‘op ‘op ‘op ¢ ‘op ‘op SIOP [occ od ‘op ‘op ‘op ‘op ‘op “op ¢ ‘op ‘op LIOb |occt ccc Aen ee ape ere od LO WO YO “10 oO “LO ¢ “‘7°9 Vsnma'unsg] o1op [occ #1q-eourns) sXuq7 | sanoyy | sanoyy | sanozy | sanoyy | sanozy (19°90) juorjooluy ery SYARUNOY L 0zI 96 aL 8h zp | paqotuy jo ide ot ao jeunuy a — SUOTYBAIOSGQ) qunoury poyPwn ee Sy “NIXOT, SONTIOLOG, WOU SOUTA GNV WAM VUNTOHT)-DOF]T AO ISA], ALIOIXOT —'T aATayv | ‘nsojOyo Jo suOISeT SGT “AOA poly “KepryOpfe “Kepungy 19d? 3 Iso] - ST N Ped PHOH pung “yors A[QISIA = Gg SAY ROY = ‘osbo YoRo UL OUT, PUGOSS UL UMOYS UOT uor)y “2 '0) po’ 110 pospuny JO sound Sosko yore ur our fy UL UMOYS O10 BJOdtO WA L Bhs ral) xs ny I 1: "4 at “Sid SNILA jo spospuny [ws9Aos JO UOLyH;NOOUL Aq poystiqeise SUM posn SNJtA oY} JO VOUO[NITA OY fz us “q pue 5 ‘gq SY suInses esajoyo-so0y jo ajdures oy1s0du107), ae wise BeRele Sete [a se ae } | | @)| Sit: i) Sa heel om | Petccal aos | bel | | | “* "18° P13 9/0°9/0°9/8 €/b' S| °°) O' SOT € | 00ST SE 86 AEGO | Pack mee > od 5 SS Pes lB Ri ilo ite al <8 Pc Be Wat ap mo iene Ws Dis i > a Bl | o) 0°G)9 1) |8'1/0°S)0 S|" © “|B T/0°S/0' S019 SSS) IS HO £07910 6/0" G|9°c| || & LOL £ 00S'T | $€ | $6 OF COL ee eo eee od Y, > SS Pic [ie WE, Se | | aed | sen sll eced pyc xX |X| xX |X eel!) See el, PS alee oe | le ee ee 5D ‘GOT “\0°6)0 10% Sor 0°30 20% 0 G|9' S|" 0° b/9'€ |b PO PPS Ce LORS aetG: Siu|ceacr on uae St sol OGGGs eo fe” od iH xX | X qexe S| Se | aes et] ew | ee | ee | a een ee | a ; rf a an) O'T/0°C)' | |G S|9 SPT) 19° Tih S0 ae £ 0°70 G0 €/8°S)0'F/9° F/B S)/9' I "| Gacor Sl ee ee St | OOL 0326 | od se oa bi al A de Se a a a aie a Se ee es a Rl a Pl ive mh es em ee a — Pe T° |0°S)}0 10's} 0319" ro" 1019 dete SLY: Vie'€ 3/P°G|0°3) "°° | OTOL £ aie * Sl St O01 9126 5S, AON i | a | | lee | |_| | | | 1zol . —_|— 2 — —|—-|—-| |---| |—- | |---| -- | |__| | - |__| — — -=— ——-— — —— ee eee a 9Z\S% | whe 8 |G | 1G) WO co 81 | LT | ST | PI etl zt | 110 a Oe) 2 119 PO38 |S | (:3'0) (sup) | uD | Eales | “noouy | gna, | UXO 1(‘0"0) |(spunog) ‘ON A a5 ; : Saas een la jo (VY edAT) | por pefay| iyo | Fey, poyepnoouy oyeqd mat —JOGWIBAOA] — ¢ SouN}eIGdW9] PUB SUOTPBAIOSG() 9401009 |yynoury| SAUTMOg | wNdeg ma ; ~dway, |joilnowy| | rae r, “ANIMG MOA ALIOITXOT, UO WAUAG VUATOH)-DOFT AO ATAWVG ALISOdWOT) AO SLSA[—"¢ ATV <{ FD a — E = ‘op ‘op ‘op ‘op ze 3 i. = ‘op [teste tess ees z anog sinoy 061 “M'O | SMOY OSL “MO | SyNoY OZT “MO | Ssanoy OZT “WO = = = _ SIQOU OG SWrO) || ie mee T 90d ae “gq edureg =. “op ‘op ‘op ‘op =. Ss ‘ a a ‘op cnet ons eee reer Z eno fe sinoy O¢T “MO | SiMoy OZ “MO | SINOY OZT “W'O | sMoY OZ “WO = = = = SIUOMOST: STO) Seo ae ae T 904 S| 2 aidures fd _— —————— as = —— poker = <4 ‘op ‘op ‘op ‘op - - _ + (0) lm | (Sita aa a gene nae Zz ajMog @ sunoy O71 “MO | S4HoyU OTL “MO | S4noYy OTE MO | sanoy OzZL MO _ - — - SIMOMVOGL cH Out cot cae een ee oiice "a ¢ S smoy 0Z1 “HO VSOt op 06tb a 5 eSInoy BP ‘Peed €S6E Ye £0EV z = OP OVel ‘op 966P = a OP. IS6€ ‘op 88EP = z SP OS6E sunoy Z2 “MO | 76h = Ee M42 OS8E Wy ofhh WM fy £9Cd = ra Wy 8VOE sinoy ZL‘ 3'O | 00OhF WM | S220Y4 Fe “peed | IctP a > Wo LVOE sinoy ZL “HO | PLEP a = ie 9VOE sinoy ZL ‘"H'°O | LObh WAM | S4noy ZI ‘peed | CLEP > = OP. SvOE sinoy 72° M'O | S9Ch = = tqo Phot oD 8crh WM ‘op 98EP = = sop SVoe sinoy 72 “"1'O | OlFh WA | SN0Y4 Fz ‘peed | Pcp = i 4oP. CVOE SOR ILeP im = p2P Tot oR 8SoP me = YQ OFOE Wy Seer = oe oP 6£65 We O8eD = = sop 8EO£ sanoy G2 "MO | €lsP a = IOP. LEOE ioe 9chr WM ioe Glep - = ‘Op 9868 sinoy ZO | FORK WAN | SiM0Y Fz ‘peed | 9LEP = x oP Soe ‘op LLGY a = sop PLOE op 8CEP =P = “OY £e6€ iS 19€h 7 = OY GEOE pon 68ED = = OP. Te6e WY b9Ch = x OR O£6E — wD Ofer =. > WY 666 sinoy @2 “MO | 90&F = = ,2P. 8668 sanoy ZL H'O | c&hh WM sunoy Sp ‘Peed | 60EP = = OP L66E Ao 9LCP = = AOP. 9C0E ‘op LSCv = = Yo SOE oye) 98CT = = ioP iZais ‘op S8cb = = CP. £66€ OR CEEV = a jOP COE ‘op OStD = = AY Tc6& sinoy ZL “MO | €9ch = = Qe 0C6E sanoy GLO | LOY WM sinoy gp ‘peed | 8SEP = = *0p 616E 22 GLEV % OR 816k oR bSeV = Ar 199 L16€ ‘op Cher a as ‘op OTG Re oe RE ee som Oh Pati Sea ees oneteetaea ¢ ‘op 10& La = sanoy 0ZT 0 Gtede: 2 [Pace Seem ge ee cree ri f ‘op Press Cd = .sanoy 96 ‘Peed PIGEON Me, ie cn ge me tee Re ees: e ‘op 80¢h = = ‘op Eos oak Sell OC ON aca oreo ha Sa > sinoy G2 "MO | c83h WM > a sinoy 0ZT ““M'O GLOGS |=. el a et ee Tangs By[nsey zjemUy s]]Nsey zjeonuy solace ser s7pNsoy zjemIUy [BOTY Ajsnoaueynoqns ‘soanypno cintpour y Rou suoTyepNoOULIaY tantpeur yeour pjo-Aep-L7 0°09 | uo YYMOIr) PSRITA pure UINIeg PIN] WO) —NIXO J £°0 YIM suorjpepnoouy jeunuy 4 SONTTALO ST WOW o1qo1ovUy 1 SOUTA anv Whit Bl9poyy-s0 Hf VIM suorpRpnooUyT yeudy ag VUTOH,)-90 0 SLSa NOLLVTNOON|—'"p Tay YT, ae nt ia “op 7 6LZP oo OS 7 eT | cae ay =a AnnS, 7 cece eaveeeeee seeeeeeteveoege reve Pr) a. oe son gy | 88 aloe 3 C00F leetisecsn: ae ea r= wh = = aenieineere Sve eC eS seen asia | rene aea | eee ae nes eles op S20 sary hitt die aioe eee ears ™ ay Peed | Per “ay dian 2p EL gill ec rmeae coer pr anes BEE a ey 2 non oye |S ie ch ep Mies ripe Fee Be, Aon See 06 ata. cee ee re et ie “op LA Ma MR Re WA ek a eae ap | ap te am pene 1 a Giada ‘op BLZP = — ‘op Beeman [aie alll clare s Se aly aia nial ean 98 ' 2 | ee aa op emia WA ORE

Ea Re em ‘op Z6CP = es op 2968 We uncheOeb See ae oe eee es Z a | ae ils pe 1) SEAR ES eR a i 8 | i = js Le | TE ea ee a eg E ‘oo tee a ES eae Ap EOL ae ape or sq[nsey eur ae + seary synsey aqPORUY | pkey] FD sy[nsoy seuny [e191 8] A[snoouvynogns ‘sernyjno =| wantpour yeour suOIe[NoOUIOYy cal a ase nih Mae ‘oo | uo YMoIX VSNaIA pue umnseg I 1B_Noouy [EMU o1qos0BUy vi9]0Y)-FOP qIIM suOyBMoOU] [eMIUy “panuijuopj—NIXOY, SONITOLOG WoOu sour, GNV WOYAG VUAIONH-SOf{ AO SLSAT NOLLWINOON|—'"f ATEV], BoruLisM AND HoG-CHOLERA SERUM 710 *jeurlue 4814 JO Yep 107Fe o[duIBs JO UOIPB[NIOUIO yy *2]qB} JO WOZ}Og IvOT o]dUIES SITY} JO UOLPETNIOUIOL BOGe “did-eournd = qx) ‘90a OFIYM= JAI Mz *A[snooueyNogns *9°9 ¢ ‘ss1d-eouINs Joy * A[[BaUOyLodBAzUL “9°9 G*Q ‘QOTUT OFIYM JO, sinoy ZL “wO ELbP WMA sinoy SP ‘peog 192 — ee errors er eseye. Bec Beet ep een. © eos cae « : ; ‘ ‘ ; ; : : ; : : : ‘ : é ; ; ; ; : ; é ; . ; . oe JO11.40") WI po AY) SLED ina a ion 661P EO aie AS. ih Ha ELC une Mey coat 6 sinoy G2 "HO | c9eP = a (OP 861F ree ete i es abr belt sek 8 sinoy Z2H'O | PIbY WAL | 20g BP ‘peo | Szeh a slg oP LQTS neal fiat ss 6, OE Ree a L el SSEP a = alo 2A a i AORN SI Tcl RP Mace Es Sa oe 9 ‘op CLer — = ‘op lk mm Ince ees mame Ue ciargang eS ¢ =P goer = les ce ol RL ee pcs Pate Sn enon ie ap Bier ile ae 1s SE ARR RMT Cro, t We Eley a J oP Dis 68lP otras & Vater fae bate 8 5. Ee? cin G sanoy 72°30 | b8Eh WA vd = ‘op SLL aes Bo Wl Re TR Spa alantae re aap Oe 0. [ smat, Fo aero ORES ORO Rio CED OT (OO Ree Os Anccr ear ral [im Go Giohceraee i hoechesel icra | escao clon ‘op CLOP Steere ene en eee e nee teen ee ee a aang Tee ae ere ALT epee choo creel | Lane re eee aie henete Local Paketemehts ovat sents] | famereeervoy vardl (oKemeteaons ‘op 990% ean SER ape Cees eo Se: ‘op OZLE wee = ‘op 68 bag art Ss EC ete le ZI sinoy 72° H'O | [8eh = = WD [esl TRE PTR AIRE AEE SC cn ies vol ‘op 80bP WM ‘op €TEVv = = ‘op Oesl pear. eee ge ee ait oe Rh ee a ol sinoy 72 °°N'O | Lobh WM sinoy gp ‘pesqd | LbhEP = = ‘op 8e8T Be ea pee era | sinoy ZZ "M'O | P9Eh = = ‘op LE8L eR RCN RRE AIM Doce iter y Tél sinoy 72°°M'O | 91bt WA sinoy gp ‘peed | Leer = = ‘op 9G8T SR les a Se gee Oct é “op 6LEb EN Zs ‘op zZR1 Rae oe Nos nae ei poten saa 6ll ‘op LEP a a ‘op 1zgl Scary ay en oarnae eke ee ll ‘op 68ch a ome ‘op 0zaI wea Ses ae A Tae Gt Cs a a LI sunoy ZL "°H'O | 168h ce as ‘op 6181 Peres te RPT ie SO NO ee oll smnoy 72 °°M'O | LObk WM sinoy gp ‘peod | FEer a = ‘op 8I8L Fe on a i ae SS ee SII ‘op Z0&F = i ‘op LT8T s Via 2 vist Se a ae II soy ZL°H'O | OFeh = = ‘op OLgt PEEPS At NA ea oe ae ell sinoy ZL “MO | Petbh WM sinoy Bp ‘Peed | 69ZF = ae ‘op SI8L ZO ee ager eee ee ean a aT EN cll ‘op ChEep = — ‘op PIgl rss ids seb heer oie ae Ill sinoy Z2 MO | O8Zh = ia ‘op jC) en Deen yr Sana re nS ll sunoy ZL “MO | bebh WM sinoy gp ‘peed | 09EP = = “op Ist See ed roe A LR En ie aad 601 ‘op LOEp = an ‘op 1181 Sey aa Signe enna eis 01 ‘op eCeh = - ‘op ONE o— |, (Poees ae colin Cahn Seu uae nata Lacon 101 ‘op OSZF oy - ‘op ZRL1 ph PAS aa cowmecetey a On On ren eae 901 simoy ZL °°W'O | L6zh ~* = ‘op 17) Ge CDR ee obtener yt col ‘op 66eh WM sinoy gp ‘peed | T8Zh = = ‘op OGD ay eee i eg ge ene nt ae i Pol ‘op boch WA ‘op ¥6zb we v ‘op Bp [estima dhe Se Uap ieo atin ale Naa els RRwIER Ol sanoy 72 "MO | OFF WA sanoy ZI ‘peed | Loeh > = ‘op 1172) CC Ma lpg aaee aati cee rar Ae WS Ae sy sol sinoy ZL “WO | OLEF ea a ‘op PPET 0 [ttn nr teense eee tena att 101 sunoy 72." N'O | I1th WM sinoYy #Z ‘peed | OFEF = == ‘op OUT ot, Salli aS Ligeia a atl Lee ee et Ma 0OT ‘op LIEP = ~ ‘op en] SANG yuna Oisiian SST aRo aaa maa ees 66 ‘op ZZeh = — ‘op 220% casei AAO eae MESS Re Aaa 86 ‘op O68 = J ‘op (10 Raa ee ere ee aes sofa ean 16 ‘op I¢eh = = ‘op 890% ahaa pe eee teg ees ts ae 06 op e1eh a - ‘op BdOG [Soest ee bee es SSRN GA Ne ae oe aie 6 s}[NseyYy ;]eanuy s}]Ns0yy | gyemyay pacer ser) s}nsoy eunnuy pee ey Ajsnooue}nogns ‘seanypno Wan peur yBwou suorje;NooUloy uinipeur ywou pyo-Aep-)] “9°70 uo YyMOIs) PSNITA PUB UWINIAgS £°0 WIA suorjpepnoouy peunay 9Iqorov Uy B9}OYD-FOF{ YIM suorjpepnoouy pPeMUy “panwijuoy)- “NIXOT, SANITALOG YOU SATA ANY WONAG VAAIONT)-NOF{ AO SLSA 7, NOLLVTQOONT—"p aay], Epw. A. Cantu, H. W. Jakeman, T: W. MuNcE 711 by planting two toxigenic strains of B. botulinus (Type A) in glucose bouillon, and after seventeen days’ contact the cultures were heated to destroy the toxin and guinea-pigs were inocu- lated. As will be seen in Table 5 one guinea-pig died on the fifth . and one on the sixth day, presumably as a result of toxin formed within the body. This is in accord with the recent work of Orr (11), since, if toxin were contained in the injected material, death would have followed in a few hours. TaBLe 5.—INocULATIONS WITH HEATED BoTuLinus CULTURES. Guinea-pig No. Date Material Results Inoculated = on oe Oh cea Aa Ra ne ee Nov. 25, 1921 | 1lce.c. of heated bouillon culture | Died, 5 days LUCY 2 5 Se Ss ee ee eee do. do. do. The heated suspension was cultured in minced meat medium to make certain that the spores had not been destroyed. All cul- tures showed profuse growth of B. botulinus in two days. That the toxin-producing ability of the spores had not been destroyed was proven by animal inoculations shown in Table 6. TABLE 6.—INOCULATIONS wiTH BoTULINUS CULTURES FROM HEATED SUSPENSION. Date Guinea- Amount Material Heated and Pig Date of Inocu- How Results Cultured No. Inoculation lated Inoculated | tl ————__—________. Bouillon cultures from heated sus- | - pension shown } in Table 5..... | Nov. 25,1921) 4204 Dec. 21,1921 0.2c.c. Subcutaneously Dead, 5 hours do. | 4205 do. do. do. do. Four bottles of phenolized anti-hog-cholera serum were inocu- lated with this toxin-free spore suspension which had _ been proven viable and toxigenic. Two bottles were inoculated with 0.1 cc. and two with 0.5 ¢.c. of the spore suspension, and all were allowed to stand at room temperature for fifteen days, if after which guinea-pigs were inoculated. All animals received My 0.5 ¢.e. subeutaneously, two receiving 18 million spores and two ik 90 million, respectively, depending upon the bottles from which + they were inoculated. The two which received 90 million died ; in six days, while those receiving 18 million died in seventeen i days, which again indicates that toxin was formed within the , body. These results, confirming as they do those which are BoruLisM AND HoG-CHOLERA SERUM 712 “Aq eoy= X *(P ZOL=P'S °F “0) Sounyesodui0} wOIy poyyU10 pospuny sOJ soandty a “AupyOHe “Avpung: “uorepNooUr Jojye BXVp JO soquINU 9} BOIpUL Fuipeoy MO[Eq MOI 484y UI spes9UIN AT; x x x | x x x foes x x x x x loco x x x x sa VPN NT | it PW 2 a lee Be SE can ——!| gs '101 ge ttt 000g ozlozlozlpzlozgloelocl pelezlozglotlogo cr 92|9%10%1| 2's x x x x x x bool xX x x x x fools x x x X pet | db (EN oP ag DS a SE el cP ede Foe ts Pe 2s Ln a Sete 6001 Pe Gee 6662 pelpeleziozlpeloglocl: ezlozlozloe| ert: oe |ozloe | oz xX xX x x x x fools x x x x x fools X x X x Se a RR ah wR | ae te | Bees fe a Ae ig se [tects 2662 Eto Derg CVS Nes bell Loca |p Mage Oe? Foyle Pes DFM 25 I OL OPPS AOS ESS eye o.9.° te OLE ots (ee x X x x x x foc x x x x x fete: x x x X Ei ea acl fk a at ln FA AS RF oa i Pee WA | Pe Gp [reese eeeeees 166% CAG Wea |) Cero NeS WQS Were SPL CoRR oe SR LE incom koa Ge | Omcn conus | ee ma @'¢|9%/| 0219'S x x x x x x oct x x X x xX Pools x x X X Se a | ee a S| | aa Eo | ee | in Gg [ttt e eter eee 9662 06. I) ed I eaeo Nl Cys Wea Wh v4etg ORS PRO oe Gin Once OM Gi |G halen Ok ico enchant [baa okt a2 | 0% | 0%] 9'S x X x x x xX fol x x x x x foobeo x x x x Se Rah Rl aR a FS a cP ee 0 Re ed ee a is gp [teres eee 6662 ORG ciecn | Once On cm Oct | MOlk can [perenne ica Ons |(1S kG Tee cece Oise acne ieee Mera SN Pg aot a 3 he peyepnoour 61 Sr We Wor VEShe Ph St) lea It | OL 6 8 L 29 | 2S v € z I | Avq ean} | (spunog) ON 31d SSS Fao | TI }Seinjesodue |, pue suoIyRAIOSq( (sna1A vsajoyo-Foy Jo UoKoNposd oy} 40J ssid porpuny [esodes JO UOrye;NVOUT oy Aq Poyst|qeyse SBA YOIYA Jo QOUO|NILA OY} ‘SNALA BIOPOYO-FOY Jo “o'O Z YIM pue ‘'0'o sod sosods UOTT]IUT Og, AjoyeuULXOIdde Fura} UOS 91N}XTUT e4Ods-CUNLOS BY} JO *9°9 GZ YIM poyepNoouL peuALUB Yous] ) “SaUOdg SANITALOG GNV WAH VUATOH’)-DOP{-LINY JO AUOLXTY, HLIM ANIMG AO NOILVTAQSONT—") ATAV 7 Epw. A. Canin, H. W. JAKEMAN, T. W. MUNCE 713 presented in Table 5, apparently indicate that botulinus toxin is not produced in phenolized anti-hog-cholera serum, since, had toxin been present, the guinea-pigs should have died a few hours subsequent te injection. In view of these findings, which agree with those of Orr (11) and others that massive doses of spores may result in the death of guinea-pigs, it was deemed necessary to determine if similar results might occur in swine. Accordingly swine weighing less than 50 pounds were inoculated with 25 ¢.c. of this serum-spore mixture. All remained well, as shown in Table 7, although they received in the injection approximately four billion spores. In order that the apparent conclusion might be substantiated by results in a larger number of animals, new mixtures of serum and spores were made in the proportions and manner previously described, and the experiment was repeated, using the same pre- cautions which were taken in the previous experiments to insure and prove the toxin-producing ability and viability of the spores. The animals, both cholera immune and susceptible, shown in Table 8, gained weight during the entire test period and showed no undesirable effects following the injection of this spore-anti-hog-cholera-serum mixture, either when used alone or simultaneously with hog-cholera virus. Each 30 ¢.c. dose con- tained approximately five billion spores. To determine whether or not B. botulinus and hog-cholera virus in symbiosis exert an action which differs from that ob- tained by a B. botulinus-hog-cholera-serum mixture as shown in Table 8, the following experiment was conducted: Spores were planted in commercial hog-cholera virus, following the technique previously described. This was injected simultaneously with anti-hog-cholera serum into both susceptible and cholera-immune swine. It will be observed in Table 9 that all animals inoculated with this material remained well. The combined action of botulinus spores and hog-cholera virus ou: susceptible pigs unprotected by hog-cholera serum was next studied to determine whether such contamination altered the syndrome ordinarily observed in cholera. The time and man- ner in which these animals sickened differed in no respeet from those of animals inoculated with virus known to be free from contamination. The results recorded indicate that spores of B. botulinus in 714 BorutisM AND HoG-CHOLERA SERUM Tasie 8.—ExpPEeRIMENT TO DETERMINE THE ACTION OF BaciLLus Botutinus Spores 1n ANTI-HoG CxHoLenA SERUM ON Born, SUSCEPTIBLE AND IMMUNE SWINE. Description | Material inoculated | Dosage Results Cholera immune | Hog-cholera-serum-spore mixture 30 c.c.! | Remained healthy do | do. do do. do. do. do. do do. do. do. do do. do. do. do do. do. do. do do do do. do do do do. do do do do. do do do do. do do. do. do. do. do. do. do. do. Cholera suscep- do. | do. do. tible do do do. do do do do. do do do do. do do do do. do do do do. do do do do. do do. do. do. do. do. do. | do. do. oO. do. : | do. do Cholera immune | Hog-cholera-serum-spore mixture do. do. and hog-cholera virus. do do. | do. do do do. | do. do do do. do. do do do | do. do do. do. do. do. do. do. | do do. Cholera suscep- | do. | do do. tible. | -do do. | do. do do do. do. do do do. do. do : do. do. | do. do. 5.7 oe ee do. do. | do. do. S185 Sate Bae do do. do do. 1 Each cubic centimeter contained approximately 180 million spores. phenolized anti-hog-cholera serum failed to produce a toxin and that swine inoculated with massive amounts of spores in anti- hog-cholera serum did not develop botulism. Orr (11) has shown that botulinus spores placed in unphenolized defibrinated guinea-pig blood develop a strong toxin within 30 hours. Since TaBLe 9.—ReEsuLts OF INOCULATION W1TH HoG-CHOLERA-VIRUS-SPORE MIXTURE. Pig No. Description Material inoculated Results DITSie tS ee Cholera immune Virus-spore mixture (2 c.c.!) and Remained healthy Hog-cholera serum (30 c.c.). TESA, 2B: do. do. do 4066 do. do do 2998: ee | do. do do 7A bf a do. do. do. PAO B45 do. do. do. 3775...... Cholera susceptible do. do. Sie. $e do. do. do 3767 do. do. | do 3776 do. do. do 3766 do. do do 57 (7d) Nee a do. do do 1Each cubic centimeter contained approximately 180 million spores. Epw. A. Cantu, H. W. JAKEMAN, T. W. MUNCE 715 this differed from our results when using a phenolized blood serum medium, the following experiment was conducted: Five- tenths of a eubic centimeter of toxin-free spore suspension was placed in 25 ¢.c. of unphenolized defibrinated hog blood and al- lowed to remain in contact for eleven days. Mice inoculated with 0.3 ¢.c. died in nine hours, whereas guinea-pigs receiving 0.5 ¢.c. died in twelve hours. It is interesting to note that the characteristic odor associated with B. botulinus was present in this unphenolized spore mixture, whereas it was never ob- served in the phenolized mixture. Although this experiment has been conducted on a small seale, the results are in close accord with those obtained by Orr (11). The striking contrast in the results obtained with phenol- ized and unphenolized defibrinated blood suggests an inhibitory action upon the toxigenic activities of B. botulinus by the phenol in anti-hog-cholera serum and hog-cholera virus. These experi- ments with phenolized and unphenolized blood are being re- peated on a larger scale. SUMMARY Examinations of 134 samples of anti-hog-cholera serum and hog-cholera virus described in this paper, supplementing 1,700 previously conducted, failed to reveal the presence of Bacillus botulinus or its toxin. B. botulinus did not produce toxin in phenolized anti-hog- cholera serum. B. botulinus in symbiosis with phenolized hog-cholera virus did not produce toxin. A strong toxin was formed in unphenolized defibrinated hog blood which had been artificially inoculated with botulinus spores. Healthy swine inoculated with anti-hog-cholera serum and hog-cholera virus artificially inoculated with toxin-free botulinus spores acted no differently from those which receive serum or virus free of B. botulinus. Susceptible swine inoculated with hog-cholera virus and botulinus spores showed no difference in temperature, symptoms, postmortem lesions nor time of sickening from those regularly used in virus production. In unphenolized hog blood in which B. botulinus is growing 716 BoruLisM AND HoG-CHOLERA SERUM the odor considered characteristic of B. botulinus was marked, whereas in phenolized anti-hog-cholera serum or virus no odor was observed. REFERENCES TO LITERATURE Jour. Amer. Vet. Med. Assoc., Oct., 1921, p. 76. Amer. Jour. Pub. Health, April, 1921, p. 352. . Jour. Med. Research, vol. 42, No. 2, p. 129. . Jour. Infect. Diseases, Sept., 1921, p. 267. Jour. Path. and Bact., Jan., 1916. Jour. Amer. Med. Assoc., May 1, 1920, p. 1220. Arch. Internat. Med., 1921, vol. 27, p. 265. Jour. Amer. Med. Assoc., Jan. 11, 1919, p. 88. Jour. Canadian Med. Assoc., 1918, p. 2. : Jour. Amer. Med. Assoc., Sent. 20, 1919, p. 907. . Jour. Infect. Diseases, Jan., 1922. pn. 118. me 09 DO KH SOnI HH =e DISAPPEARANCE OF MALARIA IN TEMPERATE CLIMATES Malaria, formerly a disease common to the temperate as well as to warmer climates, has now become practically extinct in the temperate zone and is rapidly disappearing even in warmer regions. Dr. C. Joyeux,' professor of parasitology in the Paris Medical School, has recently commented on the disappearance of the disease from France. There is evidence to show that this disease was extremely prevalent in France in past centu- ries. Now Joyeux presents evidence that the anopheles still abound in many regions, although there has been little or no malaria for generations in these districts. He calls attention to the explanation for this paradoxical fact published simulta- neously by Roubaud *? in France and Wesenberg-Lund * in Den- mark. According to these observers the mosquitoes, which used to feed on human blood, still do so in tropical countries; but in temperate zones they are attracted to barns and stables where they can find moisture, warmth and protection from the winds, and where they can feed on horses and cattle. According to Roubaud, in the course of time the mosquitoes have apparently developed a zoophile strain, being more attracted to cattle than to human blood. This has contributed to the elimination of ma- laria in the colder countries.—Journal of the American Medical Association (Nov. 12, 1921, vol. 77, p. 1578). 1 Joyeux. C. Presse Méd.. vol. 29. p. 1392 (Sept. 24, 1921). 2 Roubaud, E. Ann. Inst. Pasteur. April. 1920. ° Wesenberg-Lund. Mém. Acad. Roy. Sci., vol. 7. p. 8 (1920-21). CLARIFICATION OF HOG-CHOLERA DEFIBRINATED- BLOOD ANTITOXIN By R. R. HENLEY Biochemic Division, Bureau of Animal Industry, United States Department of Agriculture THE METHOD of preparing clear and sterile anti-hog- cholera serum described by Dorset and Henley (1) in 1916, and now in general use in the industry, is applicable only to fresh and non-phenolized defibrinated or citrated blood. For various reasons it may be desirable to clarify, concentrate, or, at times, sterilize old phenolized defibrinated-blood antitoxin. In the past this has been done either by a modification of the Banzhaf- Gibson (2) process or by the heat-salt process (3) developed in these laboratories. As those processes are expensive and are dif- ficult and tedious of operation, efforts have been made to develop a process that will serve to clarify, concentrate and sterilize old defibrinated-blood antitoxin as simply and easily as the bean- salt (1) process accomplishes those ends in the case of fresh de- fibrinated or citrated blood. In fresh defibrinated-blood antitoxin the cells, which are known to be inert, are intact, and on this account the entire cells, both the stroma and contents, consisting for the most part of hemo- globin, can be separated easily from the serum by the bean-salt process. In old defibrinated-blood antitoxin a very different con- dition exists, because of the fact that the greater part, if not all, of the cells have been broken down or hemolyzed and the liber- ated hemoglobin has passed into solution in the serum. Bean extract will agglutinate and thus facilitate the removal of the disrupted stroma, but it has no effect whatever upon the dis- solved hemoglobin, and so is of no avail in aiding its removal. It was believed that a selective precipitant for hemoglobin could be found, and a search for such a reagent was instituted. In this search the action of a great variety of materials was studied, and simultaneously a search of the literature was con- ducted. A statement by Mann (4) that chloroform would pre- cipitate hemoglobin attracted attention and promised fulfillment of the quest. While the first attempts to precipitate hemoglobin 717 > 718 CLARIFICATION OF HoG-CHOLERA ANTITOXIN with chloroform were disappointing, in that only small amounts of hemoglobin were precipitated, it was soon found that this pre- cipitation was complete (1) if the blood contained at least 0.5 per cent of phenol, (2) if the temperature of the blood did not exceed 40° F. when the chloroform was added, and (3) if the mixture of blood and chloroform was thoroughly shaken. When the factors influencing the action of chloroform on hemoglobin were understood the following process to separate clarified serum from old blood was devised. PROCESS FOR THE REMOVAL OF STROMA AND HEMOGLOBIN FROM Oup DEFIBRINATED-BLOOD ANTITOXIN Step 1. Addition of Precipitants A. Addition of bean extract to remove cells and stromata— To 500 ¢.c. of phenolized (0.5 per cént phenol) old defibrinated- blood antitoxin in a balloon flask, previously chilled to 40° F. or lower, 10 to 15 ¢.c. of bean extract (5) is added and the contents of the flask are agitated by hand. B. Addition of chloroform.—Immediately following A, 50 c.e. of chloroform is added to each 500 ¢.c. of blood and the contents of the flask are agitated by hand. C. Addition of salt-—This procedure should be used only when the serum is to be separated from the clot by filtration as described later. Promptly following the completion of B, add 5 grams of sodium chloride and transfer the flask and its contents immediately to a shaking machine. Step 2. Shaking Unless shaking promptly follows the addition of chloroform a clot will form at the bottom of the flask; therefore the transfer of the flask and its contents to the shaking machine should follow immediately the completion of Step 1. It is essential that the mixture of the blood and chloroform be agitated thoroughly in order to secure the maximum precipitating effect. For shaking, a power-driven Camp shaking machine is used, but other shaking machines will doubtless answer the purpose. The mixture is shaken for 10 minutes, following which the blood will be found to be in a semisolid clot from which a clear, light-red serum will exude on standing. Step 3. Separation of Serum The serum may be separated from the clot by centrifugaliza- tion, or by filtration. (1) Centrifugalization.—Either the continuous or bucket type centrifuge may be employed. As the blood after shaking is R. R. HENLEY 719 usually clotted into a semisolid mass, it is necessary, in order to remove it from the container, to break the clot. This may be accomplished easily if the clot is allowed to stand until the serum exudes and then is slightly shaken by hand until it is reduced to the desired fluidity. With this precaution the serum may be easily separated from the clot by either type machine. It may be mentioned, however, that it will be advisable to use a contin- uous type machine only when it is of sufficient capacity to care for a considerable quantity of precipitate. Separation with the bucket type machine is accomplished in the same manner as in the separation of serum from cells of fresh defibrinated or citrated blood by the bean-salt process. (2) Filtration.—The separation of the serum from the clotted hemoglobin may also be accomplished by filtration through paper. For filtering the serum from the clotted hemoglobin a filter tray* instead of the usual funnel has been employed advantageously. The clotted blood is filtered in lots of 1,000 ¢.c. each, as the filtrate from this amount of blood has been found to pass through one tray in 8 to 12 hours, giving a transparent, light-red serum. A yield of at least 500 ¢.c. of clear serum should be obtained from each 1,000 ¢.c. of blood at this stage, but this does not represent all of the serum present, as, because of the bulkiness of the hemoglobin precipitate, a considerable portion of the serum remains mechanically held in the precipitate. In order to recover this it is necessary to press it out of the precipitate, for which purpose the precipitate and the paper are transferred to a muslin cloth, which, after being folded into a bag-like shape, is placed in a fruit press and the mechanically retained serum pressed out. The volume of the pressings should not be less than 150 «.c. from the clot representing each 1,000 -c.c. of blood. The expressed serum is combined with the clear serum obtained by direct filtration, as above described. In case the pressings are not entirely clear, they are clarified, previous to combination with the clear filtrate, by thoroughly incorporating in the pressings 1% to 2 per cent of powdered infusorial earth and filtering the mixture through paper. This will remove effectually any trace of cloudiness from the pressings. Step 4. Heating and Phenolization The serum obtained as above described may be heated to 58° C. for one-half hour in the same manner and with the same results that attend the heating of clear serum obtained from fresh de- fibrinated blood. Although a defibrinated blood may contain 0.5 per cent phenol, the amount usually added to insure keeping, it has been found that clarified serums obtained as a product of this process contain 1Ka Ki filter trav. 720 CLARIFICATION OF HoG-CHOLERA ANTITOXIN on an average only about 0.2 per cent phenol, an amount insuf- ficient to prevent spoilage; so it is necessary to add sufficient ad- ditional phenol to bring the phenol content to 0.5 per cent. Assuming the presence of 0.2 per cent phenol, it is then neces- sary to add of 5 per cent phenol one-fifteenth of the volume of the serum to be treated. Thus, if one has 950 ¢.c., add 1/15x950, or 63 ¢.c. of 5 per cent phenol. PRECAUTIONS TO BE OBSERVED The following precautions should be observed: 1. The blood should always be chilled to 40° F. or lower before adding the precipitants required in Step 1. 2. The blood should be transferred to the shaker and shaken immediately following the addition of the precipitants. 3. Shaking should be only sufficient to form a clot. Prolonged shaking will break the clot and render separation more difficult. Experience will soon teach the proper time to discontinue shaking. . ’ 4. After the clot has formed and the flask has been removed from the shaker, the flask may be allowed to stand until the clot contracts and serum exudes. 5. Phenol should be present in the defibrinated-blood antitoxin to the amount of at least 0.5 per cent. The addition of 40 cc. of 5 per cent phenol to each 1,000 c.c. of phenolized defibrinated blood yields a product of slightly less color than if it is not em- ployed, but products obtained in this manner have not been tested for potency, and the use of this additional phenol is not recommended at this time. 6. Because of the variation in amount of hemoglobin present in old blood, the amount of chloroform to be used can not be definitely stated. Fifty ¢.c. of chloroform to 1,000 c.c. of blood has been found to be ample in all cases. The minimum amount necessary to give complete precipitation should be used. 7. The clot after separation of the serum should be sufficiently dry to crumble when handled. RESULTS OBTAINED BY THE APPLICATION OF THE PROCESS TO OLD DEFIBRINATED-BLOOD ANTITOXINS The process has been applied to a large number of old defibri- nated-blood antitoxins, as a result of which considerable data have been accumulated. In order to avoid the presentation of this rather large mass of detail, the results are merely summa- rized as follows: 1. YVields.—The yields of clarified serum by this process vary rather widely, due, in great part at least, to the wide variation in the amounts of hemoglobin that may be present. Of seventeen — ——— R.-R. HENLEY 721 old defibrinated bloods treated by the process, using centrifu- galization in some cases to separate the serum, and filtration in other cases, the yields of clarified serum obtained, before final phenolization, varied from 56 to 75 per cent of the origina! volumes subjected to treatment, and gave an average yield, be- fore final phenolization, of approximately 65 per cent. On the addition of the required amount of 5 per cent phenol solution necessary to insure a phenol content of 0.5 per cent in the fin- ished product, the average final yield was approximately 69 per cent of the original volume treated. Blood to which salt was added gave as a rule slightly increased yields over correspond- ing blood to which no salt had been added. A fresh defibrinated blood gave a yield of clear serum by the regular bean-salt method of 72.5 per cent, and the same blood after phenolization gave a yield of 67.2 per cent of clarified serum when treated by the bean-salt-chloroform process. 2. Indicated losses —A chemical analysis of bloods before and after treatment by the process indicated that about 10 per cent of the globulins, which carry the antitoxin, may have been lost. However, in the method of analysis some stromata may have been determined as ‘‘globulins lost,’’ and the figure 10 per cent may be too high. Some loss due to retention of the serum by the elot must occur. This loss may be minimized by insuring thorough separation of serum and clot. 3. Effect of the process on bacterial count.—Two old defi- brinated-blood antitoxins which contained an average of 15,000 organisms per cubic centimeter, as determined by counts made on agar plates, were subjected to treatment by the process, and the final product, on plating, was found to be sterile. 4. Effect on phenol content.2—Clarified serums obtained as products of this process contained, on an average, only 0.2 per cent of phenol, although defibrinated blood from which they were prepared contained 0.5 per cent phenol. 5. Results of tests on hogs.—As it is recognized that the bean- salt process is an effective process for the separation of a clear, potent serum from defibrinated or citrated hyperimmune blood, and as the process described herein differs from that process only in that chloroform is used, it was not considered necessary to aot conducted by Dr. F. W. Tilley, Biochemic Division. 2Mr. R. M. Chapin, of the Biochemic Division, kindly made these determinations. 7122, CLARIFICATION OF HoG-CHOLERA ANTITOXIN carry on any extended investigation as to the effect of the process on the potency of the serum. However, in order to determine if the addition of chloroform was in any way injurious, Serum 262, a defibrinated-blood antitoxin prepared in the Bureau of Animal Industry’s laboratories, was treated by the process and the prod- ucts were tested for potency. Two products were obtained: (1) A clear, unheated, phenolized serum, and (2) a clear, heated serum. The total yield of clarified serum, after final phenoliza- tion, was 80 per cent of the original volume of blood treated. On this account the clarified serum was tested in doses of 80 per cent of those of the original defibrinated blood; that is, 10 and 15 ¢.c. doses of the original untreated blood were given to pigs that also received 2 ¢.c. virus each, and 8 and 12 «.c. doses of the clarified serums were given to pigs which also received 2 ¢.c. of virus each. All pigs remained normal throughout the test ex- cept one pig that received 12 ¢.c. of clarified heated serum and one pig that received 8 c.c. of clarified unheated serum. Each of these pigs exhibited slight diarrhea on the second day of the test, but showed no other reaction. CONCLUSIONS Of the various processes for the refinement and sterilization of cld hog-cholera defibrinated-blood antitoxin which this labora- tory has tried out, some on a large commercial scale, the process described herein appears to be by far the simplest and most practical. This process has never been applied on a commercial seale, therefore anyone contemplating its employment is cau- tioned to familiarize himself with the various steps of the proc- ess by first applying it to small quantities of blood. Further- more, products obtained from such small quantities should be subjected to rigid potency tests in order to check up any losses in potency that may occur. SUMMARY The factors governing the reaction between chloroform and hemoglobin by which the hemoglobin of the blood may be pre- cipitated were studied, and a process, based upon this reaction, for the separation of a clear, sterile serum from old defibrinated- blood antitoxin was devised. 1Dr. W. B. Niles conducted the potency tests. i ; R. R. HENLEY T2060 It is shown that the yield of clarified serum separated by this process from old defibrinated-blood antitoxin approximates 70 per cent of the original volume, and that the product of the process is free from bacterial contamination. While analysis in- dicates that the globulin content of the serum suffers a slight loss, potency tests indicate that the loss of antibodies during clarification is very slight. REFERENCES TO LITERATURE. Dorset, M., and HENLEY, R. R. Jour. Agr. Research, vol. 6, No. 9, pp. 333-338. BANZHAF, F., and GiBson, R. Jour. Biol. Chem., vol 3, No. 4, pp. 253-263. U. S. Dept. of Agriculture, Rept. of the Chief of the Bureau of Animal Industry. 1918-1919, p. 53. MANN, Gustav. Chemistry of the Proteids, p. 472. London, 1906. DorseT, M., and HENLEY, R. R. Jour. Amer. Vet. Med. Assoc., vol. 50 (n. s., vol. 3), No. 6, pp. 699-702. Sop Wee he A DOUBTFUL HONOR An honorary medical degree has been conferred on former Field Marshal Ludendorff of the German Army by the medical faculty of the University of Kénigsberg in East Prussia. This news is chronicled in the Munchener Medizinische Wochen- schrift under the significant heading ‘‘Let Us Hang Our Heads.’’ In the diploma it is stated that the honorary title is given to ‘‘the hero who with the sharp blows of his uncon- quered sword protected the German people from the crowd of booty-hungry enemies.’’ The )Darmstadter Zeitung, official organ of the government of Hesse, remarks sarcastically: ** Mur- der of the masses and doctor of medicine—they go well to- gether.’’ -The attitude of evident disapproval on the part of these two influential journals goes far toward offsetting the action of the University and shows that German sentiment is not all in favor of honoring the late military “‘heroes.’’ Belgium has a state school of practical horseshoeing. Di- plomas were granted to seventeen pupils who passed the 1921 examinations. ; Investigation by the American Horse Association results in the statement that a better grade of horses appears on the streets of Boston than on those of any other city in the Union. EFFECTS FOLLOWING IMPROPER METHODS OF EXTRACTING HYPODERMA LARVZ FROM THE BACKS OF CATTLE By S. HapwEN Chief Veterinarian, Reindeer Investigations in Alaska, Bureau of Biological Survey, United States Department of Agriculture IT HAS GENERALLY been conceded by veterinarians, ento- mologists and others that the extraction of warble larve from the backs of cattle should be recommended to farmers. The benefits from the point of view of lessening the numbers of the parasites are obvious and can not be denied. As each warble fly may de- posit several hundred eggs, every larva destroyed means much toward lessening the evil. . In 1916 the writer (1) called attention to the fact that the pro- teins contained within Hypoderma larve had toxic properties when injected into susceptible animals; that they produced marked symptoms and in some cases fatal results. In 1917 Had- wen and Bruce (2) published a full description of the experi- ments. The cooperative work accomplished in Denmark in the removal of Hypoderma larve has been referred to many times in the writings on warble flies, and it would seem opportune to re- view two of the latest papers on the subject. In a general way these papers show that the extraction of warble larve is not without its attendant dangers. To the writer it seems clear that Danish veterinarians have only just lately recognized the fact that a disease they designate as rosenfeber may follow the ex- traction of the larve. Laust Brodersen (3), 1919, writes a very clear and concise paper on his observations made in a farming community in Norre Nebel, Denmark. He describes a disease in cattle which has some similarity to urticaria and which he calls rosenfeber. The affection is found during the spring and summer, and the cases are reported as occuring during the daytime or evening. Briefly the symptoms noted are as follows: Fever is present, the pulse is full, the jugulars are distended. Tremors are noticed, especially of the lips, and the animals are restless and unruly. Edemas are the rule; the eyelids, muzzle, 724 iia S. HADWEN 725 submandibular region, occasionally the udder, the anus, and vulva are affected. Tears drop from the eyes, mucus from the nose, and there is frothing at the mouth. Breathing is labored and accompanied by a hissing sound. There is slight tympanitis, and rumination is irregular. The feces are thin and are dis- charged frequently. The treatment consists of bleeding, cold applications and febrifuges. The disease is acute, usually run- ning a benign course. In 1914-15 Brodersen saw many cases of rosenfeber in cattle following the extraction of the larve. He gives notes on seven cases which he treated. They occurred from April 20 to May 31. He also treated three cases in May, June and July where no larve had been extracted. In these latter cases he wonders if the lar- ve had not been accidentally injured, and says that if this is the case, rosenfeber is a sickness caused by the absorption of poison- ous material from the larve, or, more likely still, to the foreign albuminous material in the larve, and that if this theory is cor- rect ‘‘the disease must be related to the so-called serum sick- ness.’’ Brodersen does not mention any fatal cases. In my preliminary note and in the subsequent paper with Bruce, fatal cases were described following the injection of war- ble juices. Several additional symptoms and lesions were de- seribed, such as coughing, bleeding at the mouth and anus, irri- tation of the skin, and a eyanosed appearance of the body. After the death of the animals, which was due to asphyxia, the blood remained incoagulable. These fatal cases may occur naturally, and if so, it is unlikely that veterinarians would have seen them, as death occurred in a few minutes. As soon as the findings recorded by Brodersen were under- stood by most of the farmers, he states that they became fright- ened and completely stopped the practice of extracting the larve. He believes that the people who were paid to perform this work eould not do so without damaging some of the larve, as they made only three visits to each herd during the season. The farm- ers were advised to undertake the work themselves. Professor C. O. Jensen, 1919, reviews Brodersen’s paper and recalls an experiment which he made on a calf sixteen years pre- viously. This animal was injected with the juice derived from two small Hypoderma larve. In from one-half to two hours it developed symptoms suggestive of rosenfeber. An immense 726 EXTRACTING HyPpoDERMA LARV2 FROM CATTLE edema of the eyelids and the anus occurred, also of the connective tissues elsewhere. Professor Jensen suspected rosenfeher, but did not like to eall it so on account of its occurrence at the wrong time of year. Now that Brodersen has published his observations, Jensen says that it is very probable that all cases of rosenfeber are in some way or other connected with Hypoderma larve. Jensen argues that the toxic material acts like a lymph- agogue of a transient nature. He writes at some length on the edemas found surrounding the larve in the gullet, and believes that they are not inflammatory, though Kock and De Vries con- sider that they are so. If the edemas are permanent they should produce clinical manifestations of their presence. Jensen further states that during the past years no clinical ob- servations have been brought out which would argue against the transient nature of the disease, with the exception of a single case of tympany. In this connection he mentions an interesting report by De Vries. A young animal, after feeding, suddenly became unable to swallow and was vomiting. A sound was in- troduced into the esophagus and difficulty was experienced in forcing it through the posterior end. A diagnosis of stenosis was made, probably due to a foreign body. The animal was slaugh- tered and in the posterior part of the esophagus a number of Hypoderma larvee were encountered together with edemas of the submucosa. No foreign body was found and no injury to the esophagus. Prof. Jensen concludes that it is likely that a num- ber of such cases have been misinterpreted in practice. Jensen (4) says also: ‘‘The extraction of Hypoderma larve has been considered rather safe hitherto. In cireulars issued by the German Imperial Health Department it is stated that there is no need to be afraid of injurious effects following the extraction of the larve. From different sources, however, it had been learned that urticaria might occur. Schottler saw urticaria fol- lowing in cases where the larve had been punctured and then squeezed out. He also said that by this method of treatment serious suppuration may follow. But as far as I know, no one has previously observed that the extraction may be followed by rosenfeber or similar disease.”’ The symptoms connected with Jensen’s experiment which he saw after the injection he considers came on too rapidly for the albumen to be the toxie element responsible for them. In this S. HADWEN TT latter statement no doubt Jensen. is wrong. The time he gives of one-half to two hours for the symptoms to develop is very much longer than necessary in most cases, when injections are made. In the 1916 experiments we found that in the fatal cases the results were immediate, and in the non-fatal they generally came on in a shorter period than half an hour. In some more recent experiments conducted on 10 calves, the three animals which gave marked reactions showed symptoms in fifteen to twenty minutes. Jensen’s idea that the swellings in the esophagus are transient is correct in a sense, and Hadwen and Bruce have shown that the larve move away when surrounded by edema. But they still remain in the esophagus, consequently the reaction against them still goes on, though the situations of the swellings may change. The question resolves itself largely on the degree of resistance on the part of the animal, as to whether the swellings are large or small (5). CONCLUSIONS The disease called ‘‘rosenfeber’’ in Denmark appears to be identical with hypodermal anaphylaxis. The reader is referred to articles which have appeared in this journal on this subject. Squeezing out Hypoderma larve from the backs of eattle is a good method for lessening the numbers of warble flies and has been attended with considerable success in Denmark. However, to avoid unfavorable after-effects in the animals, the extraction of the larve must not be undertaken hastily nor must rough methods be used. Above all the larve must not be injured in the process. . The walls of the sae in which the larva hes are in the nature of a defensive barrier. This sac, in addition to holding the larva, contains pus cells and bacteria in many eases. It may also hoid toxie material excreted by the larva. Therefore if the walls are lacerated a variety of reactions may take place. Softening the skin first with water and then squeezing the warble saes should remove most of the larve. In cases where a larva can not be extracted by this method a round-ended pair of forceps might be used to stretch the opening and to assist in drawing it out. After the larva has been removed the cavity should be flushed out with clean water. In the cases where a larva has been rup- 28 Extractinc HypopERMA LARV4% FROM CATTLE tured accidentally the parts must be washed quickly to dilute and remove the toxic material. \ The cooperative work in connection with the extraction of war- ble larve in Denmark had in 1919 been going on for some years, apparently without any drawbacks. At this late date, however, the Danish veterinarians and subsequently the farmers have dis- covered that troubles may follow what they thought was a per- fectly safe practice. It would seem evident that the work was undertaken without a sufficient number of properly controlled preliminary tests. It must not be thought, however, that the Danish veterinarians are to blame for not having foreseen or recognized the danger in removing the larve. The profession generally considered that the extraction of the grubs was simply a mechanical piece of — work and was quite without danger to the host. When the co- operative work began in Denmark the dangers from Hypoderma were unknown. The point the writer wishes to establish is that other countries should profit by the Danish experience. He still favors the ex- traction of the larve (and has so stated elsewhere), but he be- lieves that before laymen are advised to undertake it in a whole- sale manner further tests must be made. The symptoms of anaphylaxis are so striking, though the dis- ease is as a rule without danger of fatal results, that to a farmer it appears to be a serious matter. The cases which have been re- ported so far are few in number, but now that attention has been called to them, they may be found to be more common than was supposed. It is for this reason that careful tests should be made to settle this point. 1. HaApWEN, S. 1916. Hypodermal anaphylaxis. Jour. Amer. Vet. Med. Assoc., vol. 2, No. 3, pp. 72-74, Figs. 1-2. 2. HApDWEN, S., and Bruce, E. A. 1917. Anaphylaxis in cattle and sheep, produced by the larve of Hypoderma bovis, H. lineatum, and Oestrus ovis. Jour. Amer. Vet. Med. Assoc., vol. 4, No. 1, pp. 15-41, Figs. 1-15. BRODERSEN, LAUST. 1919. Om Rosenfeber hos Kvaeg. Maanedss- krift for Dyrlaeger, vol. 31, pp. 321-323. 4. JENSEN, C.O. 1919. Bemaerkninger on Hypodermalarvernes For- hold til Rosenfeber. Maanedsskrift for Dyrlaeger, vol. 31, pp. 324, 326. 5. HaApWwEN, S. 1918. Natural occurrence of eosinophilias. Jour. Parasitology, vol. 4, pp. 185-137. ie) LABORATORY DIAGNOSIS OF POULTRY DISEASES * By L. D. BUSHNELL and F. R. BEAUDETTE Bacteriological Laboratories, Kansas Agricultural Experiment Station, Manhattan, Kansas DURING the past few years the Department of Bacteriology has carried on investigational work in poultry diseases, and from the experience thus obtained we have been able to systematize the work considerably. While we have devised no new methods, we feel that the information obtained and the laboratory meth- ods used may be of value to others. The aim in this work has been to make a rapid and accurate diagnosis of diseased condi- tions. That an accurate diagnosis is necessary goes without question, and from our own experience we have found that such a diagnosis based upon clinical and external symptoms alone is very often impossible. As the poultry industry is one of the most important branches of agriculture, and as the people are gradually learning the value of poultry on the farm, the veterinarian should interest himself to a greater extent in poultry diseases and their cor- rect diagnosis and control. We believe that the proper use of a bacteriological laboratory as an aid to the diagnosis of poultry diseases will greatly aid the busy practitioner and improve methods for the control and eradication of poultry diseases. Since the beginning of the investigational work in this labora- tory we have found some diseases quite prevalent in this State that were not known to occur here. Fowl typhoid may be given as an illustration. The diseased specimens were sent to the lab- oratory from various parts of the State, as well as including the vicinity of the Station. A great many letters of inauiry have been received regarding poultry diseases. In some instances the description given was so characteristic that there was no doubt of the diagnosis. In other cases the description was incomplete or misleading. In replying to such letters we have suggested measures to be exe- euted until a correct diagnosis could be made. In the meantime, the owner was instructed to send to the laboratory two or three 1 Contribution No. 42 from the Bacteriological Laboratories of the Kansas Agri- cultural Experiment Station. = 129 730 LABORATORY DIAGNOSIS OF POULTRY DISEASES of the affected birds for examination. These are sent by ex- press in wooden crates, but chicks come admirably by parcel post in perforated pasteboard boxes. CLINICAL FINDINGS As soon as the live birds reach the laboratory a careful exam- ination is made, which often permits an immediate diagnosis. Such is the case with those local conditions termed roup, bum- blefoot, ete. Here the caseated mass found beneath the eyelids, the diphtheritic patches in the mouth and throat, or the wart- like growths on the unfeathered portions of the head lead to the diagnosis of roup, and the swollen foot to a diagnosis of bumblefoot. In other cases the symptoms are less suggestive and the birds must be kept until more specific symptoms de- velop or until death permits a postmortem and _ bacteriological examination. The nature of the droppings is noted, and if diarrhea occurs such diseases as cholera, fowl typhoid, botulism, and, in the case of chicks, white diarrhea, or in turkeys, black- head, are indicated. The color of the droppings is also of value in that in certain affections there is a more or less characteristic color. Much whitish material and mucus in the feces is an indi- cation of cholera, or white diarrhea in the case of baby chicks. A greenish tinge is indicative of fowl typhoid, though not of im- possible occurrence in cholera. In doubtful cases the age of the bird might aid in the diagnosis, as fowl typhoid more often oc- curs in adult birds, while cholera affects birds of all ages. The whitish droppings of a bird suffering from botulism re- semble those of a cholera fowl, but in this disease the other symptoms are so charactertistic that we need not depend upon this feature. The yellowish droppings of the consistency of thick paint are seen only in cases of blackhead. These are often designated as ‘‘sulphur’’ droppings. The color of the comb may be used as a guide in making a diagnosis, since this symptom is very reliable in cases of botulism, where a persistent bright red color is always seen. In cholera there is a marked tendency toward cyanosis, while ar anemic comb is suggestive of fowl typhoid. The comb of an individual suffering from blackhead resembles that of one af- fected with cholera. A high temperature is not as diagnostic as L. D. BUSHNELL AND F. R. BEAUDETTE ~ 731 a subnormal one. The former may indicate any of the sep- ticemias, while the latter is highly indicative of botulism. BLoop CULTURES If the septicemias are suspected an attempt is made to cul- ture the organism from the heart’s blood while the bird is still living. The individual is placed in lateral reeumbeney with the left side up. After washing thoroughly the area over the heart with a 5 per cent solution of phenol, a point on an imaginary line drawn from the tip of the sternum to the back is located where maximum pulsation can be felt. About 114 to 2 inches from the point of the sternum a 2-inch sterile needle of 22 gauge, attached to a sterile Luer syringe, is inserted into the heart and a small amount of blood withdrawn. A portion of this is put in broth containing 1 per cent citrate to prevent clotting, and another portion is streaked on an agar plate. The use of the citrated broth is of value chiefly for the isolation of Bacillus avisepticus and B. sanguinarium. From the agar plate either may be obtained. A combination is an advantage. By such a procedure the organism can in many cases be cultured and identified before the death of the bird. In case no culture is obtained the bird is still available for further study. The following record sheet is filled in for each case and a careful record kept of each bird: RECORD SHEET Case No BU) a bem eee ee CACCTING Sheen MO rr Or genet cewwiete. weet. bol.) eee ee) Py YS 8 SON ee ee ld) Se Address ne el ee ee ae Se ee, Oe ee Breed .. smi Sian sie bb en aa eGR ya rie OES Ate Tak SSE ce Leg ‘band °No Gach EAS RASS ILS MPa ce ee an ST a CORP Sige TNE, siicpieza ala lee mt Aan al NE cE BEE ARSE Lc EERE 2 IPRS © Ele Seen Wee e ROL noe okie ee Re Oe Ew CT TES ee SE AS eae ne ae eee es Re ee External eae coal cla nati es Aaa ARIE EL Slee t seibeset eaN Diagnosis .. Bea ob ope oe ox), 9 iia Prognosis .. ee Disposal ... 4 eee, Hise ea Sed Postmortem ss Weadeas Te apace i Cee Sar a ce nee eee ae Agglutination (B. ee ree) thee eee ee oe ‘Oieri tests: ....-.... pe Sore jays: (Noir Aadee dnielcns seneet it I aera eee LE DLE GTA J oa OE a I Re . Urino-genital Parasites 732 LABORATORY DIAGNOSIS OF PouLTRY DISEASES PosTMORTEM TECHNIC As soon as an individual dies a careful postmortem examina- tion is made. The feathers are thoroughly soaked to prevent dust. The bird is laid on its back and a linear incision is made through the skin from the vent forward, over the crop along the mid-ventral line. The skin is then removed from the sides and the exposed surface flamed. By means of a flamed knife, forceps and scissors an incision is made across the abdomen just behind the sternum. This is carried forward on both sides through the ribs to the anterior part of the sternum, where bone forceps are used to disarticulate the attachments. Such a flap laid forward exposes the abdomen and thorax. A_ red-hot spatula is used to sear the surface of the heart and liver. A Pasteur pipette is then used to aspirate material from these organs for cultures. In making the Pasteur pipettes a 6-mm. glass tube having thin walls is cut in 10-em. lengths. These are plugged with cotton at each end and autoclaved in packages containing three or four. These may then be stored in the labor- . atory for future use. When desired for use a tube is with- drawn from the package, heated in the middle in a Bunsen flame, and drawn out into a capillary tube. By breaking such a tube two pipettes are made. It has been found that by using the Pasteur pipette a maximum number of pure cultures can be obtained. Such a pipette is much more effective. than a wire loop, since larger amounts of material can be transferred. In taking material from the liver of birds dead for some time, the specimen should be taken in an area some distance from the surface of the intestine. The agar plates used for streaking are made from meat in- fusion with a reaction of about pH 8.0. The fluids from the tissues should be well streaked over the surface of the agar with a sterile wire loop, otherwise growth may not occur. If growth occurs on the plates, fermentation tubes containing dextrose, lactose, maltose and saccharose broth, as well as tubes of nitrate broth, peptone solution and litmus milk, are inoculated. Brom- thymol-blue is added to the fermentation tubes as an indicator. These tubes are incubated at 37°C. and observations are made daily. By means of the fermentation reactions the organism can usually be identified in 24 hours. The reduction of nitrates a L. D. BUSHNELL AND F. R. BEAUDETTE 133 is tested after Griess’s method, and Ehrlich’s aldehyde is used in testing for indol production. The culture should be allowed to incubate four days for the latter test. The following protocol will illustrate the reactions of the more common organisms isolated : | Dex- Lac- Mal-— Saccha- Nitrate _Indol | trose tose tose —s rose Reduc- Forma- Litmus Milk Name | | tion tion Ay te Gy |) A | Gi; A]G/iA G B. sanguinarium..... he — |) — | +) — 1 —)} — _ - Acid, alkaline in about 14 days. B. avisepticum....... eee a > + No change. B ip ee je ae +/+)])-!-)3 > —-|- _ ~ Acid, then alka- line in 14 days. Key to symbols: + = positive; — = negative; > = variable reactions. In cases of suspected tuberculosis an acid-fast stain of ma- terial from a lesion will suffice. A careful search is always made for intestinal parasites and the air-sac mite. Botulism has but recently received a proper amount of at- tention as a disease of poultry. The chief symptom is that of limberneck in fowls, and a laboratory method of diagnosis is quite essential. A rapid diagnosis is sometimes made as follows: Wash the contents of the gizzard into a flask of sterile salt solu- tion and after shaking the suspension thoroughly allow to stand until the coarser particles have settled. A few cubic centi- meters of this material is passed through a Berkefeld filter and 1 ce. is inoculated into each of two guinea-pigs. Inoculate one of the pigs with 250 units of polyvalent botulinus antitoxin as a control for toxic conditions not due to B. botulinus. Symptoms of intoxication often develop in the animals within twenty-four hours after inoculation. The first sign is that of a slight pa- -ralysis of the legs and the inability to swallow. The symptoms become more and more pronounced until death ensues. A new milk-producing champion for the territory east of the Rockies has been discovered in Kolrain Finderne Bess, a Hol- stein cow owned by F. F. Field, Dutchland Holstein Farms, Brockton, Mass. Her record is 32,563 pounds of milk in one year. PENNSYLVANIA INSTRUCTIONS FOR THE OPHTHALMIC TUBERCULIN TEST By T. E. Munce State Veterinarian, Harrisburg, Pennsylvania THE Bureau of Animal Industry, Pennsylvania Department of Agriculture, issues the following instructions to Bureau agents and practicing veterinarians with regard to the ophthal- mic tuberculin test: Pennsylvania does not recognize the ophthalmic tuberculin test as an official test when used alone. It is a most valuable adjunct and can be used at any time, or in combination, with- out interfering with the efficiency of the other tuberculin tests or having its results modified by their influence. TECHNIQUE As in the other tuberculin tests, the identity of each animal should be established and recorded. Examine the eyes for any abnormal conditions. Do not apply the test to animals which show any local inflammation of the eye. Ophthalmic tuberculin is prepared in both dise and liquid form. In applying the liquid, a curved glass dropper, with an outlet as small as possible, is preferable. The animal’s head is held by an assistant in such a position that when the operator drops the liquid tuberculin into the eye it will be diffused over the entire surface. Close the eyelids with the hands for a few seconds, to prevent escape of the tuberculin. In instilling the dise, place the disc between the thumb and the first finger. Remember that clean hands with short, smooth finger nails are necessary. The animal’s head must be held by an assistant in such a way that the operator can, with his thumh, place the dise well back on the eyeball, under the upper lid, and toward the outer canthus of the eye. The hand should then be placed over the eyelids for a few seconds, until the dise dissolves. APPLICATION OF TEST AND Hours OF OBSERVATIONS In making an ophthalmie test the eye should be sensitized with two or three drops of a 4 per cent solution of tubereulin, 734 T. KE. MuNcE 73: ~ or one dise when these are to be used. No record of the results of this sensitization are usually made, although sometimes a reaction occurs. Three or four days after the eye has been sensitized we apply two or three drops of 8 per cent solution of liquid tuberculin or two dises. Observations should be made at the third or fourth Results of Ophthalmic Tuberculin Test hour after the instillation of the tuberculin and continued every two hours until the twelfth or fourteenth hour. The other eye should be used as control, INTERPRETATION OF TEST AND CODE FOR RECORDING RESULTS The following ‘code is used in recording the ophthalmic test: 1. Animals showing no reaction shall be recorded at each ob- servation as N (negative). 2. Reaction shall be recorded as follows: Much pus, or a distinct purulent discharge........ ae Abundant pus, combined withthyperemia of the con- junciiva and swelling of the lids................ as The accompanying illustrations will serve as a guide in inter- preting and recording results. In observing the results of the test, do not be confused by a slight white mucous discharge that often oceurs soon aiter the tuberculin has been instilled. THE VISCERA OF THE COW? By 8S. Sisson Colleye of Veterinary Medicine, The Ohio State Unwersity, Columbus, Ohio IT is hardly necessary for me to assure you that I have no intention of attempting to inflict upon you anything like a com- prehensive account of the bovine viscera. Titles in programs must be short and therefore, as in the present case, usually include very much more than a speaker expects to deal with. The object of this paper is to present some data concerning these organs which, so far as the speaker is aware, have not been published, and some others which are not in agreement with current statements. A few physiological and clinical implica- tions may be alluded to if time permits. A little more than 20 years ago the speaker introduced into the study of the anatomy of the domestic animals the method of intravascular injection of formalin solution. It is a simple statement of fact to say that this procedure inaugurated a new era in veterinary anatomy, just as it had done in human anat- omy a few years before. The most radical revision of our views has occurred with reference to the viscera, and it soon became evident that the description of these must be rewritten. In- vesigation and consequent modification of our ideas are, of course, continuing, as must be the case in any branch of science which does not propose to die of inanition. To some this state- ment will seem a mere platitude, since they know that anatomy is a very active and growing science. Unfortunately, however, many members of our profession who should know better seem to think that the anatomical field is largely exhausted, and are satisfied with the descriptions in the literature of a generation and more ago. Such an attitude might be viewed with compla- cence were it not for the fact that many of the results of recent investigations are of great practical importance, and that much of our clinical literature contains numerous anatomical errors and solecisms, which are very perplexing and misleading to the student and young practitioner. It is also evident that our 1 Presented at the fifty-eighth annual meeting of the American Veterinary Medical Association. Denver. Colo.. September 5-9, 1921. 736 VISCERA OF THE Cow ton physiological literature contains a good many statements which are obviously incorrect or are mere assumptions which have not even the merit of plausibility. The rather iconoclastic attitude which is connoted by the foregoing remarks is not assumed for the purpose of ‘‘making an impression,’’ but simply to induce the open mind and the prompt rejection of errors which are prime factors in scientific progress. The speaker then showed a series of about fifty lantern slides, made from photographs, taken by him, of dissections and frozen sec- tions which had been embalmed by intravascular injection of 20 per cent formalin solution so as to fix the organs in their natural shape and position. The following are some of the more important features which were demonstrated: The form of the lungs and the contour of the “superficial cardiac area” on both sides of the thorax; relationship of the thoracic organs to the ribs determined by the method of serial photographs and superposition of negatives; relation of these data to auscultation and percussion. The form and position of the abdominal viscera, including the more common variations; topographic changes during pregnancy; correction of current erroneous views concerning the position and relations of the omasum and abomasum in particular; relationships of the reticulum, with clinical implications in regard to traumatic gastritis and related lesions; anatomical data with reference to the mechanism of deglutition and the movement of food in the stomach; clinical implications concerning digestive disturbances and methods of medication. The arrangement of the uterus and ovaries, and changes of form and position in the pregnant animal; other variations; implications concerning exploration per rectum. The French Société Centrale de Médecine Vétérinaire an- nounces sixteen competitive prizes, ranging from 250 to 1,200 franes, and several medals, to be awarded during 1922 to vet- inarians and veterinary students for excellence in various lines of education and research. The French Ministry of Agriculture granted to the society a subsidy of 1,000 franes for the year 1921. Such awards and recognition no doubt have an influence in encouraging veterinary progress. Two French veterinarians have recently been honored by the French Academy of Sciences. Dr. Edouard Bourdelle, professor of anatomy at the Alfort school, has been awarded a prize of 2,500 franes for his work on ‘‘Regional Anatomy of Domestic Animals,’’ and Dr. H. Velu, chief of the research laboratory of the livestock service of Morocco, has received a citation. HERD EFFICIENCY FROM THE STANDPOINT OF THE VETERINARIAN ' By Earvte B. Hopper New: York, AN. iY: WITHIN the last few years the interest shown in dairy hus- bandry in this State has greatly increased. Recently the breeders have more closely associated themselves with one an- other and the national organizations of each breed are doing much to aid the local organizations and breeders in this State. A few weeks ago I read the following from the Holstein- Friesian World: ‘‘New Jersey has a well-organized Holstein association with active breeders in charge of its management and with a field secretary, J. W. Bartlett, who has an acquaint- ance throughout the State by reason of his former connection for several years with the experiment station as a dairy extension specialist. New Jersey has not been organized on a State-wide basis very long, but the fund available for this work amounts to over $5,000 per year for three years and the State is adding to that fund right along.’ The breeders of Guernseys, Ayrshires and Jerseys are also becoming better organized. With this improved organization, proper advertising, State- wide publicity, ete., cattle owners within the State will come to realize the value of purebred livestock. There is an increas- ing number of men who realize that sections of New Jersey are ideal dairy localities. They also realize that this State is in a position to take advantage of the demands of the surrounding markets. The market for export will increase. The South is becoming more and more interested in improved livestock, and it is the belief of some breeders that the demand will be greater than the supply. With these facts in mind, the breeders of this State have been encouraged to purchase the best animals obtainable. Due ts stringent money conditions, I am sure it has been a sacrifice on the part of many; they realize, however, that when livestock of proper breeding is offered for sale that is the time to buy. 1Presented at the semi-annual meeting of the New Jersey State Veterinary Medical Society. 738 EARLE B. HOPPER 739 At two Holstein sales New Jersey breeders purchased 12 ani- mals at an average of $1,580, and from three Guernsey sales 15 animals at an average of $1,888. The highest priced animal in these five sales which was purchased to come to New Jersey cost $4,150. The general average for the five sales paid by New Jersey breeders was $1,734; the average paid by the com- bined breeders of the other States was $823. It is therefore apparent that New Jersey breeders are obtaining the best that is offered. At our last State sale in Trenton 69 animals were sold, 60 of which were repurchased by breeders in this State at an average of $314. I have not had an opportunity to com- pute the results of other sales, but from observation I am sure that the same condition exists. With this increased valuation within the State and with the increased interest of the breeders for better stock, it naturally follows that their investments in this type of animal must be protected by proper and efficient veterinary service.