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Full text of "Animal experimentation in medicine through the 18th century : an exhibition, 1 July-30 October, 1965"

ARCH 



QY 
11.1 
A598 
1965 




u. s. national library of 
medicine, hmd 

animal experimentation in 

MEDICINE THROUGH 18th CENTURY 



ANIMAL 
EXPERIMENTATION 
IN MEDICINE 



through the 18th century 




National Library of Medicine 
Bethesda, Maryland. 



ANIMAL EXPERIMENTATION IN MEDICINE 
through the l8th century 



An Exhibition 
1 July - 30 October, 1965 



IC/.^* NATIONAL LIBRARY OF MEDICINE 
History of Medicine Division 
Bethesda, Maryland 



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INTRODUCTION 



Animal experimentation may be broadly defined as the 
use of animals for the purpose of increasing knowledge of 
life processes. This exhibit shows some contributions to 
medical knowledge through such investigations, from 
ancient times through the eighteenth century. Not all are 
monuments in the history of biomedical research: included 
are several lesser works. Indeed, some are shown as ex- 
amples of animal experimentation which added little to 
what we now consider the main lines of development, but 
which were typical of their period. 

During much of the classical period, the dissection 
of cadavers was prohibited, and animals were used as 
substitutes in the study of human anatomy. The most 
important experimental animals were therefore those 
thought to be most similar to humans in structure, such 
as the ape, monkey, or the pig. Even these were neglected 
during the early medieval period, until, with the revival 
of medical education in Salerno in the 10th century, 
anatomical demonstrations were again conducted with the 
pig. Although the occasional dissection of human 
cadavers gained sanction by the. -late ,13th century, animals 
continued to serve for centuries as substitutes for the 
human body as well as for experimental purposes. Thus 
Blasius, as late as l673> complained in his tract on the 
dog of the scarcity of human cadavers and included notes 
to guide the anatomist on the structural similarities and 
differences between this animal and man. 

As the use of animals declined in the study and teach- 
ing of gross human anatomy the emphasis turned during the 
17th century to their use in comparative anatomy: Casserio, 
for example, compared vocal organs of human beings and 
many types of animals, including birds, mammals, fishe§, 
and reptiles. During the same century the study of develop- 
mental anatomy begun by the Greeks was also revived. One 
of the most important contributions was De Formato Foetu 
(l60k) 9 of Fabricius ab Aquapendente, who examined gravid 
animals, particularly sheep, and compared the embryos to 
human fetal development. 



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Living animals were first used for physiological 
experiments in antiquity. Erasistratos (d.ca. 30h B.C.) 
postulated the existence of "emanations" from living 
creatures, and in what is perhaps the earliest physio- 
logical experiment reported, put a bird in a container, 
starved it, and noted the decrease in weight. Vivi- 
section played an important role in the physiological 
work of Galen, who used apes, pigs, and dogs. The revival 
of such investigations in the l6th century is character- 
ized by the work of Vesalius, who repeated many of Galen's 
experiments on both pig ^and dog, so that the reader of 
his De Humani Corporis Fabrica (15^3) could compare his 
technique and findings with those of the Greek master. 
The 17th century saw the real development of experiment- 
al physiology in studies on circulation, respiration, and- 
digestion. 

Unlike the more basic biomedical sciences, thera- 
peutics until comparatively recent times has been based 
almost entirely on experience with humans. Although 
Paracelsus in the l6th century mentioned the effect of a 
sulphur compound on a chicken, it was not until the l8th 
century, that animals were generally used for pharmaco- 
logical trials. Experimental surgery is another develop- 
ment of the l8th century, although it was suggested much 
earlier by Vesalius: one should undertake certain dis- 
sections, he wrote, "not so much for the sake of knowledge 
of the organs as iiji order to train his hands, and to learn 
to sew up wounds." 

While practically the entire range of the animal king- 
dom has been explored, especially in the fields of com- 
parative anatomy and embryology, certain animals appear 
repeatedly in the annals of experimental medicine. The dog 
has been used continuously almost from the beginning of 
biological investigation for reasons of size, structure, 
ease of handling, and economy, since it was a common ani- 
mal with relatively little economic value. The chicken 
plays a minor but unending role in experimental medicine. 
One of the earliest identified experimental animals is a 
chicken in a Hippocratic writing dating from the 6th to 
3rd century B.C. A chicken was also the first recorded 
subject of an experiment in hypnotism made by Kircher in 
the 17th century. 



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With the rapid development of physiological exper- 
imentation in the 17th century, the smaller animals such 
as rats, mice, and rabbits figure more prominently in the 
reports, although the dog continued to be the most common- 
ly used. The "lower animals," such as frogs, lizards, 
snakes, and salamanders, were also used extensively, for 
example by Harvey during his experiments on the circula- 
tion. The frog in particular became the subject of two 
important early investigations, Marcello Malpighi's micro- 
scopical observations of capillaries in the lung and Jan 
Swammerdam's muscle-nerve preparations. The guinea pig, 
which now by extension stands for any subject of experi- 
mentation, was introduced into Europe from Peru in the 
l6th century. Fabric ius ab Aquapendente reported on an 
examination of the guinea pig as early as l6oh } but it 
did not become a common experimental animal until the 
l8th century. 

The conditions of animal experimentation did not 
materially change from the time of Galen until the intro- 
duction of anesthesia and antiseptic surgery in the 19th 
century. Here is Galen's description of his method of 
preparation for a vivisection of the spinal cord, (prob- 
ably in a dog or a pig): 

Provide yourself with a large strong knife . . . 
manufactured from exceptionally good steel .... 
The animal which you vivisect should not be aged, 
in order that it may prove easy for you to cut 
through the vertebrae. For the bones of fully 
mature animals are, on account of their hardness, 

difficult to cut Lying upon its face, it 

will be stretched out upon a board, the feet 
being secured either by means of strong straps, 
in the manner in which as you know I am accustomed 
to make them fast, using a board witg holes bored 
in it, or by the hands of assistants. 

Scientists, then as now, have been aware that animal ex- 
perimentation may cause discomfort to the subjects. In 
a poem by Robert Grove, a contemporary, William Harvey is 
reported to have expressed the following rationale which, 
in his mind, and in the minds of many, justified such 
work: 



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lt is not ferocity of mind, it is not dire 
lust that makes me cruel, nor is it the 
mercilessness of a wicked heart; but the 
sacred hunger for fame, deep within my spirit 
and in my inmost "being, which forces me against 
my will to make such experiments, and drives 
away from my breast gentle feelings. It is in 
my mind to open the dark secrets of nature, to 
inquire the causes of things once unknown; to 
release the truth long a captive in chains. 



Ellen B. Wells 

History of Medicine Division 



1. Andreas Vesalius, De Humani Corporis Fabrica (Basel, 

15^3), Bk. 7, Gh. 9, S. W. Lambert, trans., in 
Logan Clendening, Source Book of Medical History 
(New York, Hoeber, 19^2), p. lk6. 

2. Galen, On Anatomical Procedures: the Later Books 

(W. L. H. Duckworth, trans. , Cambridge, University 
Press, 1962), p. 20. 

3. S. We ir Mitchell, Some Recently Discovered Letters 

of William Harvey and Other Miscellanea (Phil- 
adelphia, College of Physicians, 1912), p. k-3 . 



ANIMAL EXPERIMENTATION IN MEDICINE 
Ancient and Medieval 

1. Hippocrates. De natura pueri. IN: Hippocrates. 

De genitura ... interprete Jo. Gorraeo. Paris, 
15^5. 

Section 29 of this work, part of the Hippocratic 
corpus but probably by a Cnidian writer, contains 
one of the earliest suggestions for an animal ex- 
periment, an embryological study, in which twenty 
eggs are set for brooding and one opened each day, 
providing an opportunity for observation of the 
developing chick. 

The first separately printed edition appeared in 
15^2. 

2. Rufus of Ephesus, fl. ca. 98-H7 A.D. De corporis 

humani partium appellationibus. IN: Aretaeus. 
Libri septem. Venice, 1552. 

In his little students' manual for learning 
anatomical nomenclature, Rufus complains that in 
earlier days, human dissection was permitted, and 
that students "learned anatomy fearlessly and with 
success." He recommends using the monkey as a 
substitute, "the one which most nearly approaches 
the man by the arrangement of bones, muscles, vis- 
cera, veins and nerves." 

This is the first printed Latin edition, translated 
by G. P. Grassi. 

3. Galen of Pergamon, ca. 129-199 A.D. Opera. Venice, 

1^90. 

One of the foremost experimental physiologists of 
all time, Galen's contributions were based on in- 
numerable vivisections and dissections of apes and 
pigs, with some use of dogs and cattle. Perhaps his 
best known work concerns the nervous system, for 
which he generally used pigs or goats. 

This is the first printed collection of Galen 's works . 



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h. Anatomia porci. IN: Me sue. Me sue vita. [Leyden], 
1523. 

Compiled for teaching purposes at the School of 
Salerno some time before 1150, it is often attri- 
buted, without basis, to one Copho, a teacher 
there. For the purposes of internal anatomy (human 
dissection still being forbidden), the pig was used, 
as "there are none so like us internally as the pig." 

l6th century 

5. Belon, Pierre, 1517?-1564. L'histoire de la nature 

des oyseaux, avec leurs descriptions. Paris, 1555* 

The revival of comparative anatomy began with the 
work of Belon, who dissected some two hundred 
diverse species of birds, and attempted to indicate 
homologous bone structures in man and bird. 

Lent by the Library of Congress 

6. Coiter, Volcher, 153^-1576. Externarum et internarum 

principalium humani corporis partium tabulae, atque 
anatomicae exercitationes observationesque. 
Nuremberg, 1573* 

A pupil of Gabriele Falloppio, Coiter was an early 
student of comparative anatomy, restricting him- 
self to skeletal structure. The principal animal 
to which he compared the human skeleton was the 
ape, following Galenic practice. Stimulated, per- 
haps, by several cases of head injuries, he made 
some experimental trephinations on dogs, birds, 
and goats. 

7. Paracelsus, 1^93-15^1 • Opera B'ucher und Schrifften 

. . . durch Joannem Huserum ... in Truck gegeben. 
Strassburg, 1603. 

In his Von den naturlichen Dingen, writ tan about 
1525, Paracelsus refers to what may be the earliest 
recorded trial of a drug on an animal. This was a 
sulphur product, which "is sweet, and liked by 



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chickens, in which it causes a quite harmless sleep." 

8. Vesalius, Andreas, 151^-156^4-. De humani corpis 
fabrica. Basle, [15^3]. 

The dog was chief subject of Vesalius' studies in 
physiology, not only for the similarity of its in- 
ternal structure to man, but also for purposes of 
comparison with some of Galen's results. Vesalius, 
by the time he was eighteen, had dissected and vivi- 
sected many animals and was already becoming a 
skilled anatomist. 

17th century 

9- Accademia del Cimento, Florence. Essays of natural 
experiments . . . Englished by Richard Waller, 
London, l6Qk. 

Although short-lived, the Accademia, founded by 
Leopold de ' Medici, was the scene of many experiments, 
including animal experiments. 

These essays first appeared in Italian in 1666. 

10. Aselli, Gaspare, 158l-l626. De lactibus, sive lacteis 

venis, quarto vasorum mesaraicorum genere, novo 
invent o. Milan, 1627. 

Aselli 's discovery of the lacteal vessels, took place 
during a vivisection of a dog in July, 1622, which 
he held originally to show some friends the func- 
tion of the recurrent laryngeal nerves an operation 

originally performed by Galen. 

11. Blasius, Gerard, l626?-l692? Miscellanea anatomica, 

hominis, brutorumque variorum. Amsterdam, 1673- 

In this little manual of anatomy, Blasius discusses 
the dog extensively as a substitute for the human 
cadaver. 



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12. Borelli, Giovanni Alfonso, l6o8-l679« De motu 
animalium. Rome, l680-l68l. 

In his monumental investigations of muscular 
mechanics, Borelli examined "body types and movements 
of animals such as fishes, birds, and quadrupeds, 
and compared them to human beings in this regard. 
His approach was based on the laws of statics and 
dynamics and took a mathematical bias, as might 
be expected, perhaps, from a pupil of Galileo. 

13- Casserio, Giulio, d. l6l6. De vocis auditusque organis 
historia anatomica. Ferrara, [l60l]. 

Casserio examined a tremendous variety of animals in 
his investigations of hearing and the production of 
sound. . These were made with human anatomy in mind; 
his work illustrates adult and fetal human dissec- 
tions, as well as dogs, cats, rats, cattle, and even 
fish . 

1^-. Courten, William, 16^-2-1702. Experiments and observa- 
tions of the effects of several sorts of poisons 
upon animals, &c. ... Translated from the Latin ms. 
Roy. Soc. Phil. Trans. 27:^85-501, 1712. 

This is one of the earlier reports of trials of 
various botanical poisons on dogs. Courten, a 
naturalist and botanist, did his work in 1678-1679? 
while studying at Montpellier. 

15- Fabric ius, Hieronymus, ab Aquapendente, 1537-l6l9« 

Opera omnia anatomica & physiologica. Leipzig, 1687. 

The first description of the formation of the chick 
in the egg since Aristotle's time is in Fabricius' 
De format ione ovi et pulli, first published in 1621. 
In his De formato foetu, published in 160^-, he ex- 
amined mammalian fetuses in detail, with particular 
attention to the sheep, and with some discussion of 
fish and snakes. His public anatomies (the term 
anatomy meaning dissection) attracted many viewers, 
who occasionally wrote home about them. Thus we 
know that on January 26, I58U, he gave a public 



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vivisection of a living gravid ewe, to the great 
interest of those present. 

16. Graaf, Reinier de, l6Ul-l673« Tractatus anatomico- 

medicus de succi pancreatici natura & usu. Leyden, 
1681. 

De Graaf made important studies on pancreatic juice 
and saliva, collecting these fluids from dogs, using 
fistulas with tubes from the quills of wild ducks. 
He was able to compare the pancreatic juice of the 
dog with that of a sailor who had died suddenly and 
reported that they had identical properties. 

This work was first published in 166k. 

17. Grew, Nehemiah, 16^-1-1712. The comparative anatomy of 

stomachs and guts begun. London, l68l. Reissued 
with his Musaeum Regalis Societatis. London, 169^ • 

Although best known, perhaps, as a plant morphologist 
and microscopist , Grew used man as a common de- 
nominator in comparing the same set of organs in a 
wide range of animals. He emphasized the necessity 
of understanding the functions of the organs in 
order to make valid comparisons. 

18. Harvey, William, 1578-1657* Exercitatio anatomica de 

motu cordis et sanguinis in animalibus. Frankfurt, 
1628. 

In the course of his investigations on the circula- 
tion of the blood, Harvey used forty-nine varieties 
of animals, and these formed the basis for his con- 
clusions. 

19. Kircher, Athanasius, 1602-1680. Ars magna lucis et 

umbrae . Rome , 

A chicken was used in the first recorded experiment 
in hypnotism, part of a series in one of Kircher' s 
works on perception and color theory. He called the 
hypnotic state " act inovoli sinus," or"brilliant 
imagination. 11 



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20. Malpighi, Marcello, 1628-1694. Opera omnia. 

Leyden, 1687. 

Malpighi' s observation of capillaries in a frog's 
lung was the first use of the microscope with sig- 
nificant scientific results, in this case verifying 
what Harvey had postulated but not seen thirty- two 
years before. 

His letter to Borelli on this subject was first pub- 
lished in l66l, as De pulmonibus. Observationes 
anatomicae. 

21. Nuck, Anton, 165O-I692. Adenographia curiosa et 

uteri foeminei anatome nova. Leyden, 1691. 

There were many theories of mammalian generation, 
but very few experiments to test them. Anton Nuck, 
a professor of anatomy at Leyden made one. He open- 
ed a female dog three days after copulation?. ligated 
the uterine horn, and observed pregnancy afterwards, 
above the ligature. He wrongly concluded that the 
embryo had been derived from the ovary without 
fertilization. 

22. Pecquet, Jean, 1622-167^ • Experimenta nova anatomica. 

IN: Munier, J. A. De venis tarn lacteis thoracicis. 
Geneva, l6^k. 

While studying at Montpellier in l6*+7, Pecquet, in 
the course of dissecting dogs, worked out the trans- 
mission route of chyle from the cisterna chyli 
through the lacteal vessels into the venous system. 

This work was first published in 1651. 

23. Redi, Francesco, 1626-1698. Esperienze intorno alia 

generazione degl' insetti. Florence, 167^. 

Redi's use of insects in a "control experiment" in- 
volved two sets of jars containing meat, one open t' 
other with gauze oyer the tops. In time, maggots 
appeared within the open jars, but on the gauze cove- 
ing the others, proving that worms did not arise 



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spontaneously in rotting meat. 

2h. Severino, Marco Aurelio, I58O-I656. Zootomia Democri- 
taea; id est, Anatome generalis totius animantium 
opificii. Nuremberg, I6U5. 

In this, the earliest comprehensive treatise on com- 
parative anatomy, Severino tried to trace analogies 
of construction in corresponding parts of various 
animals. He included the text of the Anatomia porci 
(the last time it was printed as a practical work) , 
and also wrote a section on the qualifications of an 
anatomist, including ambidexterity and ability to 
use the microscope. The anatomist, he wrote, must 
not abominate the human body, and must be ready to 
explore, with his own hands, the secrets of living 
things. 

25. Steno, Nicolaus, Bp., 1638-1686. Observationes anato- 

micae. Leyden, 1662. 

This includes Steno' s account of his discovery of the 
parotid gland while he was studying at the home of 
Blasius in 1660. He was dissecting the head of a 
sheep, and found the duct when his dissecting tool 
slipped through the gland and clinked against the 
sheep's teeth. 

26. Swammerdam, Jan, 1637-1680. Tractatus physico-anato- 

nico-medicus de respiratione usuque pulmonum. Leyden, 
I667. 

In a very involved and quite modern series of ex- 
periments, Swammerdam used dogs to demonstrate the 
movements involved in breathing. It is a classic 
in physiology. 

27. Walaeus, Johannes, l6oU-l649« Deux lettres ... du 

mouvement du chyle et du sang. IN: Bartholin, C. 
Institutions anatomiques. Paris, I6U7. 

Although at first opposed to Harvey's view of the 
circulation, Walaeus changed his opinion after ex- 
perimenting on a dog and demonstrating to his own 
satisfaction the direction of blood flow in veins. 



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His letter was first published in l6kl. 

28. Zambeccari, Giuseppe, 1653-1728. Esperienze ... in- 

torno a diverse viscere tagliate a diversi animali 
viventi. Florence, 1680. 

A pupil of Redi and a pioneer in experimental 
surgery, Zambeccari performed experimental ex- 
cisions of various internal organs on dogs and 
an occasional chicken. One dog survived four con- 
secutive operations. 

l8th century 

29. Abernethy, John, 1764-1831. Surgical and physiologi- 

cal essays. London, 1793. 

Abernethy appears to have performed some of the 
earliest, nutrition experiments, desiring to introduce 
his course of anatomical lectures "with a philo- 
sophical account of the nature of the matter, which 
composes an animal body. " He fed a rabbit with 
lettuce he had grown on flannel and distilled water, 
and observed that the rabbit rapidly declined. 

30. Adams, George, d. 1773- Micrographia illustrata. 

London, 1766. 

This served as a kind of laboratory manual for the 
many naturalists, biologists, and diletantes who 
were beginning to collect, observe, and experiment, 
as well as for anatomists and physiologists. Among 
the apparatus illustrated is a handy rack for 
stretching frog preparations. 

31. Beddoes, Thomas, I76O-I808, and James Watt, 1736-1819. 

Considerations on the medicinal use and on the pro- 
duction of factitious airs. 2d ed. Bristol, 1795. 

In the process of developing what became pneumo- 
therapy, Beddoes used puppies, kittens, and rabbits 
to test the various airs and atmospheres created 
in James Watts' newly constructed apparatus. 

32. Crawford, Adair, 17^8-1795. Experiments and ob- 

servations on animal heat. 2d ed. London, 1788. 



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Une of the earliest uses of guinea pigs was in 
the summer of 1777, when Crawford began his care- 
ful investigations of animal heat. His method 
involved water calorimetry. 

Douglas, Sylvester, I7U3-I823. Dissertatio medica 
inauguralis de stimulis. Leyden, 1766. 

This student dissertation is a rambling discourse 
on stimulation and movement, following the lead 
of Haller and Whytt. The author performed several 
experiments, using as subjects six rabbits, two 
sheep and one dog. 

Fontana, Felix, 1730-1805. Treatise on the venom of 
the viper. London, 1787* 

Fontana, naturalist to the Grand Duke of Tuscany, 
used guinea pigs, frogs, and rabbits. He tested 
the effects of viper venom on various bodily fluids 
and tissues, for example blood, tendons, and nerves, 
and often used controls. 

This work was first published in 17^7 • 

Galvani, Luigi, 1737-1798* viribus electricitatis 

in motu musculari. Modena, 1792. 

The frog was the chief subject in Galvani's studies 
of electrical phenomena in organisms. 

Hales, Stephen, l6 77-1761. Statical essays: contain- 
ing haemastaticks. Vol. II. London, 1733* 

An English clergyman, and an amateur in science, 
Hales made important contributions to the understand- 
ing of the mechanics of blood pressure. Using several 
horses, he measured the bood pressure by means of 
tubes inserted in an artery. He also used dogs and 
other animals. The horses, Hales tells us, were all 
unfit for service and would in any case have been 
destroyed. 



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Haller, Albrecht von, 1708-1777- A dissertation on 
the sensible and irritable parts of animals. Trans- 
lated from the Latin ... by M. Tissot. London, 1755. 

The multitudinous contributions of Haller, one of 
the greatest physiologists of all time, include 
a series of experiments made in Gbttingen in the 
early 1750 's, in which he distinguished between 
nerve impulse and muscle contraction. For these 
experiments, he used dogs and goats, as well as 
rats, cats, and rabbits. 

Haller, like Harvey, felt sympathy for his subjects: 

. . . and since the beginning of the year 1751 
I have examined several different ways, a 
hundred and ninety animals, a species of cruelty 
for which I felt such a reluctance, as could 
only be overcome by the desire of contributing 
to the benefit of mankind, and excused by that 
motive which induces persons of the most humane 
temper, to eat every day the flesh of harmless 
animals without scruple. 

His memoir on these experiments was originally read 
in the Academy at Gbttingen in April, 1752. 

Home, Evrard, 1756-1832. An account of Mr. Hunter's 
method of performing the operation for the cure of 
the poplitean aneurism. Soc. for the Improvement 
of Med. and Chir. Knowledge. Trans. I:138-l8l, 
1793. 

Based on his experiments with Richmond Park deer 
and with dogs, John Hunter (1728-1793) developed an 
improved method of tying off aneurysms. 

Kite, Charles, d. l8ll. Essays and observations, 
physiological and medical, on the submersion of 
animals. London, 1795* 

In 1788, Kite, a medical writer and practitioner at 
Gravesend, won the silver medal from the Humane Soci 
for his work on recovery of the apparently dead. He 



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continued his investigations on various aspects of 
drowning, using cats and dogs. 

ho. Leigh, John, fl. 1785. An experimental inquiry into 
the properties of opium, and its effects on living 
subjects. Edinburgh, 1786. 

While in Edinburgh, John Leigh of Virginia produced 
this careful, critical study of opium, testing its 
qualities oh dogs and rabbits. It did not really 
add anything new, but was typical of the dawning 
style of experimental inquiry. 

His work is dedicated to George Washington, "a man 
equally revered by the friends and foes of his 
country. 11 

^-1. Monro, Alexander, 1733-l8l7« Experiments on the nerv- 
ous system, with opium and metalline substances. 
Edinburgh, 1793- 

On the basis of his experiments with the effect of 
opium on the tissues of frogs, Monro concluded 
that nerve force was not equal to electrical force. 
By this time, the frog had become a relatively 
common experimental animal. 

h2. Murray, Andreas Johann Georg, 17^0-1791 • Commentatio 
de redintegratione partium corporis animalis nexu 
suo solutarum vel amissarum. Gbttingen, 1787* 

The restoration of injured tissue was the subject of 
a debate which took place before the medical faculty 
of Gbttingen in June of 1787- Murray, a naturalist, 
contended that most tissue could not be made to 
restore itself and gave as proof copius literature 
citations and records of his experiments on 17 dogs, 
h rabbits, and 1 chicken. He was able, in one case, 
to replace part of a tendon with a ligament. 

U3. Priestley, Joseph, 1733-180^. Experiments and observ- 
ations on different kinds of air. 2d ed. corr. 
London, 1775- 



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Priestley used rats and mice when proving the 
necessity of oxygen to the maintenance of life. 
He included in his introduction to the work shown 
some comments on the capture and care of his labora- 
tory subjects. 

kk. Smith, Thomas, fl. 1805. An essay on wounds of the in- 
testines [an inaugural essay . . . University of 
Pennsylvania] Philadelphia, 1805. 

In a rather unusual thesis, Smith, a student from the 
island of St. Croix, tried various stitching tech- 
niques on dogs, in an attempt to improve intestinal 
wound repair. Of the eleven dogs used, six survived 
and were later sacrificed for evaluations of the 
repair. 

h^. Spallanzani, Lazzaro, 1729-1799. An essay on animal 
reproductions . . . Translated [by M. Mary] London, 
1769. 

Confining himself to snails, salamanders, frogs, and 
worms, Spallanzani conducted a series of experiments 
on tissue regeneration and recorded microscopic 
observations of the reforming tissue. A man of 
great experimental skill, he also did important work 
on digestion and generation. 

This work was originally published in Modena in 1768, 
as Prodromo di un opera da imprimersi sopra le 
riproduzioni animali. 

U6. Stevens, Edward W. , fl. 1777. Dissertatio physio- 
logica inauguralis, de alimentorum concoct ione. 
Edinburgh, 1777. 

In his digestion experiments, Stevens used small 
perforated silver spheres, in which various types of 
food were placed. These were swallowed by dogs, 
sheep, and cattle, and by one man "of weak under- 
standing who swallowed stones for the amusement of 
the common people." Stevens concluded, as did 
Spallanzani at about the same time, that digestion 
was not accomplished by heat, putrefaction, or fer- 
mentation, but by a "solvent" secreted by the stomach. 



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h7- Sue, Jean- Joseph, I76O-I83O. Recherches physiologi- 
ques, et experiences sur la vitality. Paris, An 
VI (1797). 

Opposed to the use of the guillotine on the grounds 
that the parts severed might continue to live for 
some time and have sensations, Sue conducted trial 
decollations on chickens, frogs, rabbits, and 
cattle to prove his point. He also stated clearly 
that animal experimentation had valid meaning for 
human life. 

h8. Townson, Robert, fl. 1792-1799- Tracts and observa- 
tions in natural history and physiology. London, 
1799- 

The studies on. amphibian anatomy and physiology 
in this work were originally made by Townson in 
Gbttingen for his medical degree, granted in 
1795* It may have had some value as a laboratory 
aid. 

^9. Trembley, Abraham, 1710-178^. Memoires, pour servir 
a 1'histoire d* un genre de polypes d'eau douce. 
Leyden, Ifkk. 

The first view of cell division was seen in a hydra, 
by Trembley, using a hand lens. He was also the 
first to make permanent grafts of animal tissue, 
again using the hydra (discovered some 39 years 
before by Leeuwenhoek) , and thus laying the ground- 
work for experimental morphology. 

50. [Whytt, Robert] 171^-1766. An essay on the vital and 

other invo.intary motions of animals. 2d ed. , with 
corr. and add. Edinburgh, 1763* 

Probably the foremost neurologist of his time, 
Whytt, on the basis of his experiments on decap- 
itated frogs, birds, some eels, and a snake, 
suggested that the soul was equally distributed 
throughout the nervous system. He investigated re- 
flex action as well. 



U.S. DEPARTMENT OF HEALTH, EDUCATION, AND WELFARE 
Public Health Service 



NATIONAL LIBRARY OF MEDICINE 




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