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Aquatic Surveys and Assessment of Selected Springs in
the Centennial Valley, Beaverhead Co., MT

Prepared for the MT TNC Field Office
by

David M. Stagliano

Aquatic Ecologist

March 2008

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Tepee Creek looking South

t MONTANA

Natural Heritage

Natural Resource Information System
University of Montana

Project Summary

Project goals of the Aquatic Survey and Assessment of selected springs in the Centennial
Valley include: 1) to sample and assess aquatic community integrity based on
macroinvertebrate, habitat sampling and basic water chemistry data, 2) to identify and interpret
key macroinvertebrate indicators found at the sites, especially identifying SOC or unique taxa.

Habitat Evaluations.

On-site habitat assessments were conducted using the rapid assessment protocol by the BLM
Aquatic Assessment ( http://wwwl.usu.edu/buglab/forms/Bug%20Protocol%20form.pdf ).
Using this assessment, the reach was divided into 10 equally spaced transects. Parameters
recorded at each were: wetted width, 3 channel depth measurements, large woody debris and
riparian shading. Basic water chemistry parameters (temperature, pH, conductivity, dissolved
O2) were recorded prior to macroinvertebrate sampling using a Horiba H-10 water monitor.
The goal of these evaluations is to characterize local reach geomorphology, riparian and in-
stream habitat, and other characteristics that influence aquatic community integrity. Sites
ranking higher using these protocols are determined to have higher quality local-scale habitat.

Macroinvertebrate Communities.

Macroinvertebrates were collected from 10 designated transects across the reach with a 500-
micron D-frame dipnet. The method utilized was the EMAP Reach- Wide multi-habitat
protocol outlined in Lazorchak (1998). All 10 samples were composited into a bucket, and the
organisms were washed onto a 500-micron sieve, transferred to a 1 liter Nalgene bottle,
labeled, preserved in 95% ethanol and transported to the MTNHP lab in Helena for processing.
The samples were processed (sorting, identification, and data analysis) by D. Stagliano
following MT Department of Environmental Quality's protocols (MT DEQ 2005).
Macroinvertebrates were identified to the lowest taxonomic level, imported into ED AS (Jessup
2006), and biological metrics were calculated from the data using the newest multimetric
macroinvertebrate (MMI) protocols (Jessup et al. 2005, Feldman 2006).

Table 1. Impairment determinations from the MMI
and O/E (RIVPACS) models (taken from Jessup 2005,
Feldman 2006).

Ecoregion

Mountain

Low Valley

Eastern Plains

RIVPACS

>0.8 or< 1.2
<0.8 or > 1.2

>63
<63

>48
<48

>37
<37

Impairment Determination

Not impaired
Impaired

Metric results were then scored using
the Montana DEQ bioassessment
criteria and each sample categorized as
non-impaired or impaired according to
threshold values (Table 3). The
impairment threshold set by MT DEQ
is 48 for the Low Mountain/ Valley
Index, thus any scores above this
threshold are considered unimpaired .
Most of these Centennial sites should

be categorized as Low Mountain / Valley sites, but we will run the metrics using the DEQ
Mountain MMI as an alternate method. The macroinvertebrate MMI score is based upon a
series of metrics that measure attributes of benthic macroinvertebrate communities regarding
condition changes to a stream system (in the form of anthropogenic caused changes).

Sites

All sites for this TNC assessment lie in Beaverhead Co., MT within former Centennial
Livestock Ranch lands or adjacent to the ranch, including a DNRC State Section. Habitat
assessments, water quality measurements, macroinvertebrate surveys were performed at 4
predetermined spring (stream) sites (per conversation with Nathan Korb) and an additional 2
stream sites. Three longitudinal sequence stream reach sites were sampled in Murphy Creek;
while 2 were sampled in Fish to Metzel Creek. Three lotic types were delineated within the
study area: Small Warm Spring Influenced Stream, Small Cold Springs and Small Foothills
Streams.

Table 2. Spring Station information. Elevation in feet. Water temp in °C, Cond=conductivity in
jus/sec. HBI=Habitat Health assessment rank by riparian/stream evaluations (++) good-excellent,
(+) fair-good, (-) poor, (- -) degraded. Bug Sample (+) = taken at site, (-)-not taken.

Spring

Name

Lat

Long

Elev

H 2
Temp

Cond

HBI

Bug
Sample

Murphy Creek headcut

44.7006 -

111.8782

6831

27.2

598

7.58

Murphy spring Right
Seep

44.7002 -

111.8781

6826

28.4

605

7.58

Murphy Creek @ Side
Spring

44.6997 -

111.8791

6820

29.4

564

7.64

Murphy Creek below
culvert

44.6987 -

111.8801

6799

26.8

594

8.05

Murphy Creek Leaving
Ranch

44.6949 -

111.8821

6742

24.8

587

7.3

Metzel Spring Source

44.7024 -

1 1 1 .8897

6810

26.7

597

8.18

Metzel seep downstream

44.7023 -

1 1 1 .8907

6800

22.8

602

8.26

Dulany Spring

44.7039 -

1 1 1 .8930

6824

22.9

606

7.99

- +

Combined springs-Fish
Creek meadow

44.7015 -

1 1 1 .8923

6764

14.5

620

8.05

springs forming Fish
Creek meadow

44.7000 -

1 1 1 .8926

6752

14.9

622

8.08

Metzel Creek

44.6754 -

111.7515

6758

13.2

399

8.8

Metzel Creek above road

44.6955 -

111.8971

6713

4.5

428

8.6

Fish Creek DNRC parcel

44.6996 -

111.9081

6776

12.7

388

7.89

Fish Creek DNRC parcel

44.7008 -

111.9108

6786

12.5

374

7.9

Fish Creek upstream
Willow riparian area

44.7022 -

111.9161

6838

12.5

382

7.9

Tepee Creek

44.6809 -

1 1 1 .7537

6690

9.1

187

7.05

Hellroaring Creek Spring

44.6157 -

111.5488

6817

7.5

269

6.78

Results

Habitat Evaluations.

Highest site riparian habitat scores were measured in the Fish Creek willow dominated riparian
reach on the DNRC state section. Lowest riparian integrity scores were also recorded from
Fish Creek at a cattle crossing and a culvert site, and spring channels flowing to Metzel Creek.
Tepee Creek scored moderately degraded. For the spring sites, Metzel spring ranked highest in
ecological condition, Murphy spring sites were fair to good & Dulany Spring was impacted by
excavation, but the in-stream habitat ranked good and was similar to some sections of Murphy
Creek springs. Water quality parameters, conductivity and pH, were fairly uniform (fall within
a normal range) across all spring sites ranging from low conductivity at Tepee Creek (187
|ns/sec) to higher values at Metzel Cr. down from the confluence of the springs (622 |us/sec).
Warmest temperature measured was 29.4°C at a side spring to Murphy Creek (Table 2).

Fish Communities.

Fish were not specifically targeted in our surveys, but we did record 2 fish species from the
spring sites, the longnose dace (Rhinichthys cataractae) and the mottled sculpin (Cottus
bairdii). Longnose dace were quite abundant in the warm spring reaches of Murphy Creek,
Metzel and Dulany Springs; whereas the sculpin were only recorded in the cooler temperatures
downstream from the warm water inputs (Fish and Metzel Creeks) were the water temps were
below 22 degrees C.

Macroinvertebrate Communities.

Overall, 74 macroinvertebrate taxa were reported from 9 samples within the TNC Centennial
Spring and Creek Sites. Average macroinvertebrate taxa richness per site was 15.7 and the

highest taxa richness reported at Dulany
Spring was 22 taxa.

One introduced species were reported, the
wide-ranging amphipod, Hyalella azteca
(from all Murphy Creek spring sites). No new
species or species of concern (SOC) were
collected, although some interesting taxa are
reported across most "warm- spring" sites that
are not usually found in typical mountain
stream habitats. These "spring-only" taxa
included Chimarra cf. utahensis,
Ochrotrichia, Helicopsyche borealis (see
drawing), Fallceon quilleri and others (inset
Table). Low numbers of individuals (<300
ind.) were collected at Tepee, Dulany and
Metzel Creek (above road culvert site). Since
the stream area where macroinvertebrate
collection takes place is fairly standardized,
these low numbers usually indicate
impairment in stream habitat or water quality.
Using the Montana DEQ multimetric index
(MMI), 8 of the 9 samples were ranked non-
impaired (good to excellent biological

Unique "Cool" Taxon

Spring
Sites

Fish Cr.
ups

Stoneflies

Sweltsa sp.

Zapada cinctipes

Mayflies

Ameletus similior

Fallceon quilleri

Caddisflies

Rhyacophila brunnea gr.

Hesperophylax cf. designatus

Helicopsyche borealis

Ochrotrichia

Chimarra cf. utahensis

Neophylax rickeri

Neophylax splendens

Dragon/Damselflies

Argia
Ophiogomphus severus

+
+

True Flies

Pseudochironomus

Pagastia
Radotanypus
Eukiefferiella pseudomontana

+
+
+

Drawing of Helicopsyche borealis, the
snail-cased caddisfly, abundant in
most Centennial warm-spring sites.
©Ethan Nadeau.

integrity), and 1 was moderately impaired (Metzel Creek
above the culvert) (Table 3). Although, Tepee Creek
should also be ranked impaired by the MMI, but low
numbers of organisms (red-flag) can eschew the metrics
and cause score inflations. Tepee Creek also had the lowest
number of taxa, most of which are tolerant to disturbance.
The upstream reach of Fish Creek has the only
macroinvertebrate community that resembles a high quality,
cold-water trout stream community (Table 3). Hellroaring
Spring also contained a fair number of sensitive cold-water
taxa (Appendix A). Community results from the habitat,
fish and macroinvertebrate surveys combined to rank the
following sites from highest biological integrity to lowest
within their aquatic ecological classification codes:

Overall Aquatic Ecological System Site Condition (in order of highest to lowest integrity):

Small Warm Spring Influenced Stream-(AES S003)-l) Murphy Creek Spring, 2) Metzel

Spring, 3) Dulany Spring

Small Cold Spring Stream-(AES S003c)-1) Hellroaring Spring

Small Foothills Stream (AES D001)-l) Fish Creek (ups Willow Riparian Area), 2) Metzel

Creek at Culvert, 3) Metzel Creek at Road , 4) Metzel Creek (below meadow), 5) Fish Creek

cattle crossing, 6) Tepee Creek

Table 3. Aquatic integrity ranking of all inventoried sites. Total number of invertebrates (# of
ind.), total taxa richness (T_Taxa), LVAL and MTN MMI scores, (++) = high biological
integrity, (+) = good integrity, (-) = slightly impaired, (--) = moderate to severely impaired
biological community. Shaded-cells represent good to excellent scores above set thresholds.
NC= scores not recalculated.

Spring

Name

T-Taxa

#of
Ind

LVAL
MMI

MTN
MMI

MMI Rank

1a
1b
1c

Murphy Creek Spring

headcut

Murphy Creek @ Side

Spring

Murphy Creek below culvert

14
16
16

366
367
309

57.7
53.5
59.1

nc
nc
nc

+
+
+

Metzel Spring

247

56.3

Dulany Spring

*157

60.3

Metzel Creek above culvert

Fish Creek upstream Willow
riparian area

10
20

*147
405

28.4

18.4

50.8

69.4

Tepee Creek

*102

*76.6

41.1

Hellroaring Spring

298

64.7

Murphy Creek furthest upstream @ headcut

Key Environmental Factors: Spring discharge permanence. Grazing — moderate- severe impacts,

Hydrology-upstream dams or diversions in the watershed.

Ecoregion: Centennial Basin (17af) typical

Rare or Unique Species: No rare species or communities documented

Rare Features: A warm spring feed by numerous side springs in the high-elevation Centennial valley.

Introduced/Exotic Aquatic Species: The amphipoda, Hyalella azteca recorded

Overall Ecological Condition: Fair-with high restoration potential, cattle regime management

Moderately impacted by cattle grazing, grazer increasers in the riparian zone-Baltic rushes and Carex

nebraskensis dominate.

Reach Geomorphology: Single thread spring channel dominated by cobbles, pebbles that are slightly

embedded by fine sediments; a warm-spring flowing through lush, dense beds of bittercress and

watercress.

Fish Community: Longnose Dace, incidentally collected. Probably the only native species in the

valley able to survive within a warm springs creek.

Fish Community Quality: IBI= Expected fish community?

Macroinvertebrate Community: Good Macroinvertebrate Quality, dominated by the transitional

cool-water stream community (Stagliano 2005), including some species not typically collected in

mountain streams — Chimarra, Ochrotrichia, Helicopsyche borealis, Fallceon quilleri

Macroinvertebrate Community Quality: MT MMI= 57.8

Murphy Creek mid-way to headcut @ side spring

Key Environmental Factors: Spring discharge permanence. Grazing — moderate impacts, Hydrology—

upstream dams or diversions in the watershed.

Ecoregion: Centennial Basin (17af) typical

Rare or Unique Species: No rare species or communities documented

Rare Features: A warm spring feed by numerous side springs in the high-elevation Centennial valley.

Introduced/Exotic Aquatic Species: The amphipoda, Hyalella azteca recorded

Overall Ecological Condition: Fair-with high restoration potential, cattle regime management

Moderately impacted by cattle grazing, grazer increasers in the riparian zone-Baltic rushes and Carex

nebraskensis dominate.

Reach Geomorphology: Single thread spring channel dominated by cobbles, pebbles that are slightly

embedded by fine sediments; flowing through lush, dense beds of bittercress and watercress.

Fish Community: Longnose Dace, incidentally collected. Probably the only native species in the

valley able to survive within a warm springs creek.

Fish Community Quality: IBI= Expected fish community?

Macroinvertebrate Community: Good Macroinvertebrate Quality, dominated by the transitional

cool-water stream community (Stagliano 2005), including some species not typically collected in

mountain streams — Chimarra, Ochrotrichia, Helicopsyche borealis, Fallceon quilleri

Macroinvertebrate Community Quality: MT MMI= 53.5

■Kr*^

Murphy Creek @ and down from culvert diversion

Key Environmental Factors: Spring discharge permanence. Grazing — moderate impacts, Hydrology-

upstream dams or diversions in the watershed.

Ecoregion: Centennial Basin (17af) typical

Rare or Unique Species: No rare species or communities documented

Rare Features: A warm spring feed by numerous side springs in the high-elevation Centennial valley.

Introduced/Exotic Aquatic Species: The amphipoda, Hyalella azteca recorded

Overall Ecological Condition: Fair-Good-with high restoration potential, cattle management

Reach Geomorphology: Single thread spring channel avg. wetted width 1.75 m, dominated by cobbles
& pebbles (a few boulders) that are slightly embedded by fine sediments; 60% riffle, 30run and 10%
pools. Less aquatic beds of bittercress and watercress like upstream.

Fish Community: Longnose Dace, incidentally collected and abundant. Probably the only native

species in the valley able to survive within a warm springs creek.

Fish Community Quality: IBI= Expected fish community?

Macroinvertebrate Community: Good Macroinvertebrate Quality, dominated by the transitional

cool-water stream community (Stagliano 2005), including some species not typically collected in

mountain streams — Chimarra, Ochrotrichia, Helicopsyche borealis, Fallceon quilleri

Macroinvertebrate Community Quality: MT MMI= 59.2

Murphy Creek leaving the Ranch

Key Environmental Factors: Spring discharge permanence. Grazing — moderate- severe impacts,

Hydrology-upstream dams or diversions in the watershed.

Ecoregion: Centennial Basin (17af) typical

Rare or Unique Species: No rare species or communities documented

Rare Features: A warm spring feed by numerous side springs in a high valley.

Introduced/Exotic Species: The amphipoda, Hyalella azteca probably exists here.

Overall Ecological Condition: Fair-with high restoration potential, cattle regime management

Moderately impacted by cattle grazing, grazer increasers in the riparian zone-Baltic rushes and Carex

nebraskensis dominated

Reach Riparian Ranking: BLM= NA EPA RBP= NA

Reach Geomorphology: Single thread warm spring channel avg. wetted width 2.75 m, dominated by

cobbles & pebbles (a few boulders) that are slightly embedded by fine sediments; 30% riffle, 50% run

and 20% pools. Less aquatic beds of bittercress and watercress like upstream.

Fish Community: Longnose Dace, incidentally

collected and abundant. Probably the only native

species in the valley able to survive within a warm

springs creek.

Fish Community Quality: IBI= Expected fish

community?

Macroin vertebrate Community: Did not collect

a Macroinvertebrate sample here. Probably the

same community as upstream sites (temps only

decreased 3°), especially with the water veg. beds

present— Chimarra, Ochrotrichia, Helicopsyche

borealis, Fallceon quitted

Macroinvertebrate Community Quality: MT

MMI= NA

Metzel Creek spring @ source

Key Environmental Factors: Spring discharge
permanence. Grazing — no noticeable impacts.
Ecoregion: Centennial Basin (17af) typical
Rare or Unique Aquatic Species: No rare species or
communities documented

Rare Features: A warm spring arising out of a rocky
outcrop in the high-elevation Centennial valley.
Introduced/Exotic Aquatic Species: None recorded
Overall Ecological Condition: Good, with proper cattle
grazing management

Reach Geomorphology: Single thread warm spring channel
avg. wetted width 1.5 m, dominated by cobbles & pebbles (a
few boulders) that are slightly embedded by fine sediments;
50% riffle, 40% run and 10% pool, flowing through lush,
dense beds of bittercress and watercress. At spring orifice-

3m WW

move

dispersed

spring area,l

degree

warmer-27.6°

Fish

Community:

Longnose

Dace,

incidentally

collected. Probably the only native species in the valley

able to survive within a warm springs creek. Fish

Community Quality: IBI= Expected fish

community?

Macroinvertebrate Community: Good

Macroinvertebrate Quality, dominated by the

transitional cool- water stream community (Stagliano

2005), including some rarely collected species not typical in mountain streams — Chimarra,
Ochrotrichia, Helicopsyche borealis, Fallceon quilleri
Macroinvertebrate Community Quality: MT MMI= 56.3

Metzel side channels after thrashed meadow

Key Environmental Factors: Spring discharge permanence. Grazing — moderate- severe

Hydrology—upstream dams or diversions, channelizations in the watershed.

Rare or Unique Species: No rare species or communities documented Rare Features

Introduced/Exotic Species: None recorded
Overall Ecological Condition:
Fair-Poor-with restoration
potential, restore main channel
flow, cattle grazing regime
management, banks sloughing-
needs riparian stabilization.
Moderately impacted by cattle
grazing, grazer increasers in the
riparian zone, Carex nebraskensis
dominated.

Reach Geomorphology: Single
thread channel, avg. wetted width
2.0 m, dominated gravels and silt
(a few cobbles) that are
moderately embedded by fine
sediments; 20% riffle, 60% run
and 20% pools.
Fish Community: Longnose
Dace, incidentally observed

Fish Community Quality: IBI= Expected fish community?

Macroinvertebrate Community: Did not collect a Macroinvertebrate sample here

Macroinvertebrate Community Quality: na

impacts,
: None

Dulany Spring @ source

Key Environmental Factors: Spring discharge

permanence, excavated out source area potentially

adding sediments to the channel. Grazing — slight

impacts.

Rare or Unique Aquatic Species: No rare species or

communities documented

Rare Features: A warm spring arising out of an

excavated outcrop in the Centennial valley.

Introduced/Exotic Aquatic Species: None recorded

Overall Ecological Condition: Fair, with proper

cattle grazing regime management

Reach Geomorphology: Single thread warm spring

channel avg. wetted width 3.25 m, dominated by

pebbles and gravel (a few cobbles) that are slightly

embedded by fine sediments; most top reach flowing through lush, dense beds of bittercress and

watercress- 100% run. At spring orifice- H20 warmer-25.6°

Fish Community: Longnose Dace, incidentally collected. Probably the only native species in the

valley able to survive within a warm springs creek.

Fish Community Quality: IBI= Expected fish community?.

Macroinvertebrate Community: Good Macroinvertebrate Quality, dominated by the transitional

cool-water stream community (Stagliano 2005), including some rarely collected species not typical

in mountain streams — Chimarra, Ochrotrichia, Helicopsyche borealis, Fallceon quilleri

Macroinvertebrate MT MMI= 60.3

Metzel Creek @ road and upstream from culvert

Key Environmental Factors: Spring discharge permanence. Grazing — moderate impacts, Hydrology-

upstream dams or diversions in the watershed, channelization of flows, Riparian stabilization.

Rare or Unique Species: No rare species or communities documented

Introduced/Exotic Aquatic Species: None recorded

Overall Ecological Condition: Fair- with high restoration

potential, cattle grazing management

Reach Geomorphology: At the road, channel widened

by cattle trampling (photo right -2.5-3m WW). Single

thread spring channel avg. wetted width 2.5 m, dominated

by cobbles & pebbles (a few boulders) that are slightly-

moderately embedded by fine sediments; 40% riffle, 50%

run and 10% pools.

Fish Community: Did not see any fish in this reach while

sampling.

Fish Quality:

Expected fish
community
would be
presumably a
trout stream
community.

Macroinvertebrate Community: Poor Macroinvertebrate
Quality, dominated by an impaired transitional cool-
water stream community (Stagliano 2005).
Macroinvertebrate MT MMI= 28.4

moderate- severe impacts, Hydrology— upstream dams or

Tepee Creek

Key Environmental Factors: Grazing

diversions in the watershed-not likely.

Ecoregion: Centennial Basin (17af) typical

Rare or Unique Species: No rare species or

communities documented

Rare Features: No rare features documented

Introduced/Exotic Aquatic Species: None recorded

Overall Ecological Condition: Poor-with high

restoration potential, cattle regime management

Heavily impacted by cattle grazing, high livestock

use index, thrashed and grazed riparian zone-
Baltic
rushes and
Carex
nebraskensi
s dominates.
Reach

Geomorphol

ogy: Single thread spring channel dominated by 100% fine
sediment; a cool- spring flowing into the sand dunes area of the
Centennial before disappearing in the sediments and eventually
joining with Red Rock Ponds. Surrounding bank materials are
similar to the channel bed materials.

Fish Community: No fish species collected or sighted in the
reach. Expected fish for this stream type have not been
met.

Fish Community Quality: IBI= 0/E= / 2.7 or 0% of the
expected fish community

Macroin vertebrate Community: Poor Macroinvertebrate Quality, dominated by tolerant species.

Macroinvertebrate Quality: MT MMI= Eschewed value due to low invertebrate numbers

Hellroaring Spring

Ecoregion: Centennial Basin (17af) typical

Rare or Unique Species: No rare species or communities documented

Rare Features: No rare features documented

Introduced/Exotic Aquatic Species: None recorded

Reach Riparian Ranking: BLM= NA EPA RBP= NA

Reach Geomorphology: Cobble-boulder dominated, 1.5 m channel width, cold-spring feeding into a

series of ponds before joining Hellroaring Creek approximately Vi mile downstream. A high-integrity,

cold- spring macroinvertebrate community collected.

Fish Community: No fish species were collected, none expected.

Fish Community Quality: IBI= No expected fish community

Macroinvertebrate Community: A cold-water macroinvertebrate community with indicator

species similar to the pristine mountain community (Stagliano 2005).

Macroinvertebrate Community Quality: MMI= 64.7

Acknowledgements

We would like to thank The Montana Field Office of the Nature Conservancy, especially
Nathan Korb and Brian Martin (MT). Accommodations were supplied by the Centennial
Livestock Ranch house owned by the Nature Conservancy. Field work was assisted by Linda
Vance and Scott Mincemoyer.

All photos in the report were taken by MTNHP personnel, unless otherwise noted.

Literature Cited

Feldman, D. 2006. Interpretation of New Macroin vertebrate Models by WQPB. Draft

Report. Montana Department of Environmental Quality, Planning Prevention and

Assistance Division, Water Quality Planning Bureau, Water Quality Standards Section. 1520 E.

6 th Avenue, Helena, MT 59620. 14 pp.
Holton, G. D., and H. E. Johnson. 2003. A field guide to Montana fishes, 3rd edition. Montana Fish,

Wildlife, and Parks, Helena.
Jessup, B., J. Stribling; and C. Hawkins. 2005. Biological Indicators of Stream Condition in Montana

Using Macroinvertebrates. Tetra Tech, Inc. November 2005 (draft).
Jessup, B. 2006. Ecological Data Application System (ED AS) Version MT 3.3.2k A User's Guide.

Tetra Tech, Inc.
Lazorchak, J.M., Klemm, D.J., and D.V. Peck (editors). 1998. Environmental Monitoring and

Assessment Program - Surface Waters: Field Operations and Methods for Measuring the

Ecological Condition of Wadeable Streams. EPA/620/R-94/004F. U.S. Environmental

Protection Agency, Washington, D.C.
Montana Department of Environmental Quality (DEQ). 2005. Sample Collection, Sorting, and

Taxonomic Identification of Benthic Macroinvertebrates. Water Quality Planning Bureau.

Standard Operation Procedure (WQPBWQM-009).
Stagliano, David, M. 2005. Aquatic Community Classification and Ecosystem Diversity in

Montana's Missouri River Watershed. Report to the Bureau of Land Management. Montana

Natural Heritage Program, Helena, Montana. 65 pp. plus appendices.

http ://w w w .mtnhp . org/reports . asp#Ecolog y

Appendix A. Macroinvertebrate Species List for all Centennial samples. Number of individuals
(abundance) collected at each site. Grey Shaded -Spring Indicator taxa. Underlined = Coldwater
Dependent taxa , Red Shaded are introduced species.

Site Name

site_code

Taxon

Abundance

Dulany Spring

UM_S0256

Argia

Dulany Spring

UM_S0256

Chimarra cf. utahensis

Dulany Spring
Dulany Spring
Dulany Spring
Dulany Spring

UM_S0256
UM_S0256
UM_S0256
UM_S0256

Corynoneura
Cricotopus
Enallagma
Erpobdella

13
4

Dulany Spring

UM_S0256

Fallceon quilleri

Dulany Spring
Dulany Spring
Dulany Spring
Dulany Spring

UM_S0256
UM_S0256
UM_S0256
UM_S0256

Gyraulus parvus
Hydrobius
Libellula
Micropsectra

15
3
2
3

Dulany Spring

UM_S0256

Ochrotrichia

Dulany Spring

UM_S0256

Ophiogomphus severus

Dulany Spring
Dulany Spring

UM_S0256
UM_S0256

Paramerina
Physella

9
22

Dulany Spring
Dulany Spring

UM_S0256
UM_S0256

Pseudochironomus
Radotanypus

7
12

Dulany Spring
Dulany Spring
Dulany Spring
Dulany Spring
Dulany Spring

UM_S0256
UM_S0256
UM_S0256
UM_S0256
UM_S0256

Sciomyzidae
Simulium
Sphaerium
Thienemannimyia gr.
Tubificidae

1
2
5

Metzel Spring
Metzel Spring

UM_S0257
UM_S0257

Argia

Chimarra utahensis

52
14

Metzel Spring
Metzel Spring
Metzel Spring

UM_S0257
UM_S0257
UM_S0257

Cricotopus

Cricotopus bicinctus gr.
Enallagma

16
3
2

Metzel Spring

UM_S0257

Fallceon quilleri

Metzel Spring

UM_S0257

Fossaria

Metzel Spring

UM_S0257

Helicopsyche borealis

Metzel Spring
Metzel Spring

UM_S0257
UM_S0257

Helophorus
Libellula

1
4

Metzel Spring

UM_S0257

Ochrotrichia

Metzel Spring
Metzel Spring

UM_S0257
UM_S0257

Paramerina
Physella

4
39

Metzel Spring

UM_S0257

Pseudochironomus

Metzel Spring
Metzel Spring
Metzel Spring
Metzel Spring
Metzel Spring

UM_S0257
UM_S0257
UM_S0257
UM_S0257
UM_S0257

Rheocricotopus
Stratiomys
Thienemannimyia gr.
Tubificidae
Tvetenia bavarica Gr.

1
2
4

20
2

Metzel Creek @ Road

UM_S0258

Argia

Metzel Creek @ Road

UM_S0258

Cricotopus

Metzel Creek @ Road

UM_S0258

Fossaria obrussa

Metzel Creek @ Road

UM_S0258

Gyraulus

Metzel Creek @ Road

UM_S0258

Hesperophylax

Metzel Creek @ Road UM_S0258 Hyalella azteca 21

Metzel Creek @ Road

UM_S0258

Hydrobius

Metzel Creek @ Road

UM_S0258

Hydroptila

Metzel Creek @ Road

UM_S0258

Parametriocnemus

Metzel Creek @ Road

UM_S0258

Physella

Metzel Creek @ Road

UM_S0258

Psychoronia

Fish Creek uDStream

UM S0258UP

Ameletus

Fish Creek upstream

UM_S0258up

Antocha

Fish Creek upstream

UM_S0258up

Baetis tricaudatus

Fish Creek upstream

UM_S0258up

Brachycentrus amehcanus

Fish Creek upstream

UM_S0258up

Cinygmula

Fish Creek upstream

UM S0258up

Drunella doddsi

Fish Creek upstream

UM S0258up

Drunella arandis

Fish Creek upstream

UM S0258up

Ephemerella excrucians

Fish Creek upstream

UM S0258up

Helicopsvche borealis

Fish Creek upstream

UM S0258up

Hesperoperla pacifica

Fish Creek upstream

UM_S0258up

Isoperla

Fish Creek upstream

UM_S0258up

Lepidostoma unicolor

Fish Creek upstream

UM_S0258up

Micrasema bactro

Fish Creek upstream

UM_S0258up

Optioservus

Fish Creek upstream

UM_S0258up

Orthocladiinae

Fish Creek upstream

UM_S0258up

Pericoma

Fish Creek upstream

UM S0258UP

Rhithroaena

Fish Creek upstream

UM S0258UP

Sweltsa

Fish Creek upstream

UM_S0258up

Taeniopterygidae

Fish Creek upstream

UM S0258UP

Zapada cinctipes

Murphy Creek Spring

Headcut

UM_S0261

Argia

Murphy Creek Spring

Headcut

UM_S0261

Chimarra utahensis

Murphy Creek Spring

Headcut

UM_S0261

Cricotopus

Murphy Creek Spring

Headcut

UM_S0261

Cricotopus trifascia gr.

Murphy Creek Spring

Headcut

UM_S0261

Fallceon quilleri

Murphy Creek Spring

Headcut

UM_S0261

Gyraulus parvus

Murphy Creek Spring

Headcut

UM_S0261

Helicopsyche borealis

212

Murphy Creek Spring Headcut UM_S0261 Hyalella azteca 1 2

Murphy Creek Spring

Headcut

UM_S0261

Hydroptila

Murphy Creek Spring

Headcut

UM_S0261

Ochrotrichia

Murphy Creek Spring

Headcut

UM_S0261

Ophiogomphus severus

Murphy Creek Spring

Headcut

UM_S0261

Physella

Murphy Creek Spring

Headcut

UM_S0261

Radotanypus

Murphy Creek Spring

Headcut

UM_S0261

Simulium

Murphy Creek Spring

down

UM_S0259

Argia

Murphy Creek Spring

down

UM_S0259

Chimarra utahensis

Murphy Creek Spring

down

UM_S0259

Cricotopus

Murphy Creek Spring

down

UM_S0259

Cricotopus trifascia gr.

Murphy Creek Spring

down

UM_S0259

Fallceon quilleri

Murphy Creek Spring

down

UM_S0259

Gyraulus

Murphy Creek Spring

down

UM_S0259

Helicopsyche borealis

125

Murphy Creek Spring down UM_S0259 Hyalella azteca 21

Murphy Creek Spring

down

UM_S0259

Hydroptila

Murphy Creek Spring
Murphy Creek Spring

down
down

UM_S0259
UM_S0259

Ochrotrichia
Ophiogomphus severus

3
21

Murphy Creek Spring
Murphy Creek Spring
Murphy Creek Spring
Murphy Creek Spring

down
down
down
down

UM_S0259
UM_S0259
UM_S0259
UM_S0259

Paramerina
Physella
Simulium
Tubificidae

12
2
2

Murphy Creek Spring

mid-way

UM_S0260

Argia

Murphy Creek Spring

mid-way

UM_S0260

Chimarra utahensis

Murphy Creek Spring
Murphy Creek Spring

mid-way
mid-way

UM_S0260
UM_S0260

Cricotopus
Cricotopus trifascia gr.

6
3

Murphy Creek Spring

mid-way

UM_S0260

Fallceon quilleri

Murphy Creek Spring

mid-way

UM_S0260

Gyraulus parvus

Murphy Creek Spring

mid-way

UM_S0260

Helicopsyche borealis

144

Murphy Creek Spring

mid-way

UM_S0260

Hyalella azteca

Murphy Creek Spring

mid-way

UM_S0260

Hydroptila

Murphy Creek Spring
Murphy Creek Spring

mid-way
mid-way

UM_S0260
UM_S0260

Ochrotrichia
Ophiogomphus severus

13
16

Murphy Creek Spring
Murphy Creek Spring

mid-way
mid-way

UM_S0260
UM_S0260

Paramerina
Physella

3
22

Murphy Creek Spring

mid-way

UM_S0260

Radotanypus

Murphy Creek Spring
Murphy Creek Spring

Hellroaring Spring
Hellroaring Spring
Hellroaring Spring
Hellroaring Spring
Hellroarinq Sprinq

mid-way
mid-way

UM_S0260
UM_S0260

UM_S0263
UM_S0263
UM_S0263
UM_S0263
UM S0263
UM_S0263
UM_S0263
UM S0263
UM S0263
UM_S0263
UM S0263
UM_S0263
UM S0263
UM_S0263
UM_S0263

UM_S0269
UM_S0269
UM_S0269
UM_S0269
UM_S0269
UM_S0269
UM_S0269
UM_S0269
UM_S0269

Simulium
Tubificidae

Baetis tricaudatus

Cricotopus

Diamesa

Eukiefferiella pseudomontana

Hesperophvlax desianatus

Heterlimnius corpulentus

Hydrobaenus

Neophvlax rickeri

Neophvlax splendens

Pagastia

Polvcelis coronata

Rhyacophila brunnea gr.

Sweltsa

Thienemanniella

Tipula

Callibaetis

Corixidae

Dicrotendipes

Leptophlebia cupida

Notonecta

Odontomyia

Psectrocladius

Stagnicola elodes

Stratiomys

4
6

Hellroaring Spring
Hellroaring Spring
Hellroarinq Sprinq

47
3
40

Hellroarinq Sprinq

Hellroaring Spring
Hellroarinq Sprinq

128
14

Hellroaring Spring
Hellroarinq Sprinq

1
6

Hellroaring Spring
Hellroaring Spring

Tepee Creek
Tepee Creek
Tepee Creek
Tepee Creek
Tepee Creek
Tepee Creek
Tepee Creek
Tepee Creek
Tepee Creek

1
1

12
22
11
5
2
2

23
20
5

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Full text of "Aquatic surveys and assessment of selected springs in the Centennial Valley, Beaverhead Co., MT"

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