ALCOHOL 631 5. "Ferrous Sulphate 20 per cent solution.—Dissolve 5 H, oE ferrous sulphate FeSojHaO, in 15 ml. of distilled water. Add 8 ml, of concentrated sulphuric acid (2) and make upto 25 ml. Keep in a stoppered flask. 6, Final Titrating Solution (red reducing 'fluid),—To 35 ml. of the 50 per cent sulphuric acid (3), add 15 ml. of the 0.1 per cent methyl orange solution (4) and 1 ml. of the 20 per cent ferrous sulphate solution (5). Mix well and cool to room temperature. This red final titrating solution keeps only three or four days, but the solutions from which it is prepared are stable for several weeks, Method,—Before boginnit^g the determination, adjust the pH of xirine samples to approximately 8,0 by the addition of a few drops of strong sodium hydroxide solution. For this purpose use a phcnolphthalein test paper, which should show a faint pink colour at the correct $>H. Place 5 ml. o£ the standard potassium dichromate solution (1) in the special flask and slowly add 5 ml. of concentrated sulphuric acid (2), cooling the flask during the addition of the acid, Place 0,1 ml, of the urine or blood in the small glass cup attached to the stopper. Insert the latter in the flask and fasten it with the springs; allow the flask to stand for 4 hours at 37°C. Bemove the stopper and titrate with the final titrat- ing solution (6)» During the titration, the dichromate solution must not be diluted with water iu any way, even by washing down the sides of the flask, A faint pink colour indicates the end point, All assays must be carried out in duplicate and must be accom- panied by duplicate blanks, treated in exactly the same way, except that nothing is added to the cup. ¥ Standardize with final titrating solution (6) against the standard didhromate solu- tion (1), with iiach assay as follows: Add 5 mi of concentrated sulphuric acid (2) to 5 ml of the dichromate solution (1), cool the flask, us before, to approximately the temperature at which the assay titrations are carried out and titrate immediately against the final titrating solution (6). Calculation,'—Lot the equivalent of the 5 ml, of dichromate be S ml o£ red reducing fluid, Let the direct titration of the blank be A ml Let the sample titration be B ml* A wmN H The alcohol in mg* per 100 ml zz..............g.......*" X 500, The declared result is to be the average of a pair of parallel duplicates, Note—This method cannot be used if Ketone bodies are present and hence preli- minary tests for their presence should be mad©* The ICozelka and Hine (Macro) method for Alcoholic Estimation is also recom- mended—for details se© B,MA, Special Committee report on ' The recognition of intoxi- cation ', 1954, pp. 33-34, Medico-Legal Points,—In European countries cases of alcoholic poison- ing are very common and are mostly accidental. In India, they are more frequent in big cities than in towns and villages, but fatal cases are very rare, I have seen only two cases of death occurring from acute alcohol poisoning among passengers who were picked up dead from railway trains at Agra Station. Whisky bottles were found iti the belongings of both. Probably their death was hastened owing to the excessive heat of the sum** mer. I had also had occasion to hold a post-mortem examination on the body of a Hindu male, aged 30 years, who died from excessive drinking of alcohol in one night on or about the 2nd October 1933. A ca$e^ occurred in Bombay where a Parsi, aged 50 yeara, committed suicide by taking a large quantity of alcohol A dock worker of Bombay Q who l«ft home in the morning returned at noon " dead drunk ", picked up a household knife, loudly declared that he wns going to md his life and died as a result of self-inflicted deep wound on the chest. Applied to*,ttejdstoi, ,al<?ohol jproduees redness and irritation, especially If it is prevented from evajx>ra£lon7P"TF'has the power of abstracting water from the tissues and precipitating proteins. Taken by the mouth alcohol is quickly absorbed by the stomach and the small intestine, and circulates in the blood* The absorption of alcohol is facilitated, if it is swallowed rapidly in a concentrated solution on an empty stomach, and it is delayed if a weaker solution is slowly drunk in, the 5. The Free Press Jow% April 15, 1933, 6, The, Bombay Sentinel, April 18, 1955.