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Full text of "Kupffer's vesicle and its relation to gastrulation and concrescence .."

QC 



\F006169 




KuppFER's VESICLE 



AND ITS 



RELATION TO GASTRULATION AND 
CONCRESCENCE, 



FRANCIS BERTODY SUMNER. 



[Reprinted from Memoirs New York Academy of Sciences, Volume II, Part II, J900.1 



Submitted in partial fulfillment of the requirements for 

the Degree of Doctor of Philosophy in the Faculty of 

Pure Science, Columbia University 



EXCHANGE 





NEW YORK ACADEMY OF SCIENCES. 
MEMOIRS, VOLUME II, PART II, 1900. 



ARTICLE III. 



VESICLE 



AND ITS 



RELATION TO GASTRTJLATION AND 
CONCRESCENCE. 



FRANCIS BERTODY SUMNER. 



TEXT FIGURES 1-34. 



NEW YORK : 
PUBLISHED BY THE ACADEMY. 



Submitted in partial fulfillment of the requirements for 

the Degree of Doctor of Philosophy in the Faculty of 

Pure Science, Columbia University 



JLOGY 

LIBRARY 





PRESS OF 

THE NEW ERA PRINTING COMPANY 
LANCASTER, PA. 



!- 



[New York Academy of Sciences. Memoirs, Vol. II, Part II, pp 47 to 83, October 15, 1900.] 



FRANCIS BERTODY SUMNER 

BORN AUGUST i, 1874. 

MINNEAPOLIS ACADEMY, 1887-1890. 
UNIVERSITY OF MINNESOTA, B. S., 1894. 

Scholar in Animal Biology, 1893-1894. 
State Natural History Survey, 1892-1893. 
University Fellow, 1894. 

COLUMBIA UNIVERSITY, 18951901. 

University Scholar, 1897-1898. 

John D. Jones Scholar, 1898. 

Senff Zoological Expedition to Egypt, 1899. 

University Fellow, 1899, (resigned.) 

University Table at Naples Zoological Station, t&$ 

Tutor in Natural History in the College of the City of New 
York, 1899. 

Member of New York Academy of Sciences. 



X. NOTE ON METHODS ...TTTTTTTrrrrr^TTTTTTTTTT T5~ 

XI. SUPPLEMENT 79 

BIBLIOGRAPHY. . . 80 



299288 






BIOLOGY 
LIBRARY 

G 



[New York Academy of Sciences. Memoirs, Vol. II, Part II, pp 47 to 83, October 15, 1900.] 



KUPFFER'S VESICLE AND ITS RELATION TO GASTRULATION AND 

CONCRESCENCE. 

FRANCIS BERTODY SUMNER. 

(Read February 12, 1900.) 

[Text figures 1-34 ] 

CONTENTS. 

PAGE 
INTRODUCTION 

I. THE TELEOST GASTKULA 48 

Gotte's view 

A new factor 50 

II. KUPFFER'S VESICLE 52 

The prevailing view 52 

Relation of Kupffer's vesicle to the " Prostomal thickening" 53 

Kowalewski's account of Kupffer's vesicle 53 

Conclusive evidence of the preceding view 54 

Previous observations of a similar nature 55 

Condition in Amia 57 

III. RELATION OF PROSTOMA TO GERM LAYERS 59 

Prostomal thickening and gut-hypoblast 59 

The relation of the gut epithelium to the notochord 60 

Historical 61 

IV. SIGNIFICANCE OF PROSTOMAL INVAGINATION 62 

V. CONCRESCENCE AND CONFLUENCE 68 

Problem stated 68 

Experiments of Morgan and Kopsch '. 70 

Conclusions 72 

VI. FURTHER MORPHOLOGICAL CONSIDERATIONS 73 

VII. YOLK VESICLES 74 

VIII. THE FUNCTION OF KUPFFER'S VESICLE 76 

IX. RECAPITULATION 77 

X. NOTE ON METHODS 78 

XI. SUPPLEMENT 79 

BIBLIOGRAPHY.... 80 



299288 



48 MEMOIRS OF THE NEW YORK ACADEMY OF SCIENCES 

INTRODUCTION. 

This paper is the outcome of observations carried on during the past few years 
in the Zoological Laboratory of Columbia University, in the Marine Biological Lab- 
oratory at Wood's Holl, in the John D. Jones Laboratory at Cold Spring Har- 
bor, 1 in the Stazione Zoologica at Naples, and finally in the Department of Natural 
History in the College of the City of New York. I owe much to the help of those 
in charge of each of the laboratories named, being especially grateful to Profes- 
sor BASHFORD DEAN for his constant interest in my work, his ever-ready assistance, 
and his many valuable suggestions. I also take pleasure in acknowledging the gen- 
erous aid given me by Professor ULRIC DAHLGREN, of Princeton University, to 
whom I owe my success in obtaining the eggs of Noturus ; and in expressing my ap- 
preciation of the help and advice given me by Doctor PAUL MAYER at Naples, as 
well as of the valuable material procured through the efforts of Doctor Lo BIANCO. 
The eggs of Salvelinus I owe to the kindness of Mr. CHARLES WALTERS, of the New 
York State Fish Hatchery at Cold Spring Harbor, and to the courtesy of the U. S. 
Fish Commission. 



I. THE TELEOST GASTRULA. 

Gotte's View. The account of gastrula formation in the teleost advanced by 
GOTTE ('73 his main idea had been briefly presented four years before) has been 
accepted by the majority of investigators in this field. Although it is now a com- 
monplace to embryologists, I shall introduce this discussion by a brief statement of 
GOTTE'S view, since it is my object to prove that this view must be amended in one 
very important particular. 

FIGURE 1. 





Figures 1 to 4 (A and B) illustrate the orthodox account of gastrula formation. 
They show the teleost blastoderm, in surface view and in section, at four different 

. ' As John D. Jones Scholar of Columbia University. 



SUMNER: KUPFFER'S VESICLE 



49 



stages of development. Figure 1 represents the early segmented germ-disc. In the 
stage shown in figure 2 the blastoderm has increased in area but diminished in 
thickness, and a thin superficial layer of cells (" Deckschicht," GOTTE) has become 
differentiated. In the stage shown in figure 3 an important change has taken place 
in the relative thickness of the parts. The central region has become thinner, both 
relatively and absolutely, while the marginal region is now thicker. The natural 

FIGURE 2. 





inference is that a part of the central cells have migrated peripherad, and this is the 
inference drawn by GOTTE. This thickened marginal region is called by GOTTE 
"Randwulst" (von Baer). It is seen in the figure that the superficial layer 
(" Deckschicht ") extends a trifle beyond the cells of the marginal wall and that 
its border rests directly on the yolk, leaving a small space, triangular in section, 
around the margin. Finally in figure 4, gastrulation is shown in progress. From 
the marginal wall a projecting ledge of cells is growing inward over the yolk. This 
process begins on one side of the blastoderm (the future posterior end of the embryo) 



FlOUEE 3. 





but soon extends to the whole circumference, although remaining most conspicuous 
on the embryonic side. Figure 4, A, represents a blastoderm of this stage as seen 
in surface view. The invaginate layer (" Secundiire Keimschicht," GOTTE) is seen to 
take the form of a diaphragm with an excentric and not quite circular aperture. The 
" Deckschicht," as before, ends freely on the yolk. Figure 4, B, is a section cut in 
the plane of the dotted line in A. 



50 



MEMOIRS OF THE NEW YORK ACADEMY OF SCIENCES 



As to the mechanics of the process, GOTTE maintained that the same peripherad 
movement of the cells which produced the " Randwulst " produced an involution of 
the margin to form the " Secundiire Keimschicht," and led to the growth of the lat- 
ter centrad. 

Although other writers have held, and probably with truth, that at least in 
some cases, the secondary layer is formed wholly or in part by ddamination from the 
germ-wall, nearly all ernbryologists are agreed that the end result is as shown in 
figure 4. A circular sheet of cells, inflected at its margin, and covered by a pave- 
ment layer which does not share in this inflection such is their teleost gastrula. 
According to this view the germ-ring or inflected layer represents the entire hypo- 
blast and mesoblast of the embryo. 

A New Factor. One object of the present paper is to show that the above 
conception of gastrulation in the teleost, although in large degree true, fails to 

FIGURE 4. 





recognize a highly important factor. The fish gastrula presents a far more complex 
structure than the scheme of Gotte contemplated. 

More than a year ago, in a paper before the American Morphological Society 
(December 28, 1898) the writer described for the cat-fish blastoderm a pronounced 
thickening of the pavement layer (" Deckschicht ") on the embryonic (posterior) 
margin of the blastoderm. Although stating that this structure was of constant 
occurrence, I was at that time unable to offer an opinion as to its significance. I 
have later devoted a great deal of attention to this question and have discovered 
this same problematic cell-mass in a considerable number of fishes belonging to 
widely different families. In all these cases, the origin and fate of this cell-mass 
appears to be the same. It is perhaps most readily observed in the trout ; but 
although this fish has long been a classical object for embryological study, the 
appearances which I am about to describe have been noticed by only one previous 
writer (Berent, '96). 1 

1 BERKNT'S observations were quite incomplete and his interpretations entirely incorrect. Even GREGORY ('99) 
the latest of those who have discussed the early development^ of the trout, has missed the point as completely as any of 
his predecessors. 



SUMNER : KUPFFER'S VESICLE 



51 



Figure 5 represents a sagittal section through an early blastoderm of the brook 
trout (Salvelinus fontinalis), 1 absolute age unknown. One of the most obvious and 
striking facts is the condition of the pavement layer at the posterior (embryonic) 
margin. Its cells, instead of being thin and flattened as elsewhere, are here elon- 
gated in a vertical direction. The surface of the blastoderm at this point is notice- 



FlOURE 5. 



Pr, 



^M^^v^l^/^^ 



?:>: i&Jsffi*'s'$< & *' - : J>^">i '^'M'^W 
'.'ri^y*'. ^.y.'a-if^-^-'w-.r >; '/ J >^ : -;. - .< /"..-:^s^.i{, 

W^iiK fe;: ^" 




After camera lucida drawing. Pr, thickening of pavement layer at posterior border. 

ably indented, and the arrangement of the cells with reference to the indentation is 
such as to strongly suggest an invagination occurring here. The line separating the 
" Prostomal Thickening," as I shall henceforth call this proliferation of the super- 
ficial layer, and the underlying cells of the blastoderm is not at all clear in this 



FlGtTEE 6. 



Pr 




Sagittal section (after camera Incida drawing) showing condition in Snleelinus blastoderm considerably 

more advanced than that shown in Figure 5. 

section. The deeper stain of the superficial cells nearly disappears as we pass inward 
from the surface. 

At a somewhat later period (a day or less older) the germ-ring is well established. 
It will now be seen (figure 6) that the " prostomal" mass of cells is continued for- 
ward into a thin layer underlying the cells of the germ-ring proper. 

1 It cannot be objected that the difference may be due to my having studied a different species of trout, since, as we 
shall see, the condition described seems to be a universal one among the Tdeontei. 



52 



MEMOIRS OF THE NEW YORK ACADEMY OF SCIENCES 



II. KUPFFER'S VESICLE. 

The Prevailing View. In the present paper I cannot enter into a complete 
historical review of this subject. KUPFFER'S own conception of this structure we 
shall consider later. In the meantime, I shall state briefly the accounts given by 
AGASSIZ and WHITMAN ('84) who first satisfactorily described the condition in the 
pelagic type of egg and by HENNEGUY ('88) who was a pioneer worker in this line 
upon the trout. AGASSIZ and WHITMAN observed in the pelagic egg of Ctenolabrus, 
a number of small vacuoles, appearing in the periblast beneath this region of the 
embryo. These vacuoles soon united into one (so much had already been observed 
by KINGSLEY and CONN, '83) and above it the cells of the embryo began to arrange 
themselves as a columnar epithelium which arched over this cavity. In the case of 
the trout embryo, HENNEGUY states that the formation of the vesicle is preceded by 



FIGURE 7. 



FIOUKE 8. 





Section showing fully formed Kupffer's 
Vesicle in the trout (after Henneguy). 



KV. 



Embryo of Clenolabrus, showing fully formed Kupf- 
fer's Vesicle. Kv, |Kupfer's Vesicle, GHy, gut-hypo- 
blast. 



a radial arrangement of certain cells in the posterior undifferentiated region of the 
embryo. A cavity then forms in the midst of these cells, thus giving rise to the 
vesicle. 

Figure 8 is drawn from one of my own preparations of Cienolahrws. AGASSIZ 
and WHITMAN give no satisfactory figures, although their description is clear. 
Figure 7 is taken from HENNEGUY'S paper on the trout. Both of these figures 
represent the vesicle in its fully developed condition. They differ in that the vesicle 
of the trout is from the first completely bounded by cells, while in the pelagic egg 
the cellular wall is not complete. But the accounts agree in representing the waP 
of the vesicle as differentiating in situ from a homogeneous mass of cells without 
reference to any preexisting (cellular) structure. 

It is now well established that both types of Kupffer's Vesicle as above figured 
actually occur : the question as to which type is more primitive will be considered 
later. Both of the above accounts, although true as far as they go, are incomplete 



SUMNER: KUPFFER'S VESICLE 53 

in so far as they neglect the first steps in the formation of this interesting structure 
and leave out of consideration certain phenomena of high theoretical importance. 

Relation of Kupffer's Vesicle to the "Prostomal Thickening." To return 
to Salvelimisat a stage considerably later than that shown in figure 6, we have the 
condition represented in figure 9, which, like the preceding figures, is drawn from a 
median longitudinal section of the embryo. In the anterior portion of the section 
there are three layers, not counting the overlying pavement layer, viz. : dorsally, 
the neural axis, ventrally the single layer of gut-hypoblast, and between the two, 
the disc-shaped cells on the notochord. The neural and chordal portions lose their 
identity caudad in a homogeneous cell-mass. The ventral layer is continued back- 
ward into one or both walls of Kupffer's Vesicle. The condition caudad to the 
vesicle is less clear. It seems certain, however, that at this stage there is no distinct 
ventral layer extending to the posterior margin. 

The position of this vescicle strongly suggests that it bears some relation to the 
mass of cells which we have discussed under 
the designation of " prostomal thickening." 
Its walls are one or both continued cephalad 
into the thin layer of gut hypoblast under- 
lying the chorda. Moreover, this prostomal 
cell-mass, unless it be represented by the 
walls of the vesicle, has quite lost its iden- 

. Posterior end of sagittal section of brook trout 

tlty. Lhe most Conclusive evidence I shall embryo (camera lucida drawing). Kv, Knpffer's 

defer, however, until I have entered into a Veaicle : ^, neural axis; Ch, notochord; any, 

gut-hypoblast. 

brief historical discussion. Let us consider 

whether the structure herein termed " prostomal thickening " has been previously 

observed. 

Kowalewski's Account of Kupffer's Vesicle. M. VON KOWALEWSKI described 
in Carassius a mass of cells, triangular in section, lying at the posterior margin of 
the embryo and bounded by the pavement layer, the marginal wall and the periblast. 
Except at this point, the pavement layer spanned a narrow space and ended freely on 
the yolk as above represented. In his first paper (KOWALEWSKI '85) he considered 
this cell-mass to be the rudiment of the whole entoderm, considering the cells of the 
germ-ring to be purely mesodermal in their fate (he asserted this same view in his 
next paper, '86, a). Later ('86, b) he modified his view to the effect that it repre- 
sented only that part of the entoderm forming the walls of Kupffer's Vesicle. The 
latter, according to KOWALEWSKI, was a part of the archenteron, and the cord of 
cells which he figures (figure 10, B) as extending backward from below the vesicle 




54 



MEMOIRS OF THE NEW YORK ACADEMY OF SCIENCES 



was the solid rudiment of the neurenteric canal. The radial arrangement in this 
problematic cell-mass at an earlier stage (figure 10, A) suggested to him an in vagi- 
nation, and he considers this to be the equivalent of the " prostoma " of KUPFFER, 
differing from it only in that the latter opened directly to the surface instead of 
being covered by the superficial layer. Kupffer's Vesicle represents, according to 

FIGURE 10. 





A. Sagittal section through posterior border of 
blastoderm of Carassius (after Kowalewski) show- 
ing supposed invagination occurring at this point. 



B. Section showing Kupffer's Vesicle in Caras- 
sius (after Kowalewski). 



FIGURE 11. 



KOWALEWSKI, a small but important part of the " Gastruladarm," the rest of which 
is represented by the uncovered part of the yolk. 

It is surprising, in view of this brief and accurate description presented as long 
ago as 1886, that the prostomal thickening has been all but ignored by subsequent 
investigators. Both HENNEGUY ('88) and VIRCHOW ('95) have seen cells in this po- 
sition, but do not regard them as being of constant occurrence nor of any theoret- 
ical importance. (Concerning BERENT, see below.) 

Conclusive Evidence of the Preceding View. The 
present writer first noticed the prostomal mass of cells in 
the blastoderm of Amiurus (figure 11), regarding it at the 
time as a mere thickening of the superficial layer. Upon 
following out the subsequent course of development and 
comparing with the condition in other forms, I was led to 
a view of its significance quite similar to that expressed in 
KOWALEWSKI'S later paper, even before I had seen the lat- 
ter. The presence of the nick or indentation often found 
in this cell-mass, and the grouping of its cells (figures 5 and 10, A) have been noted 
by KOWALEWSKI and offered by him as an evidence of invagination. Another ap- 
pearance, which I noticed in an embryo of Amiurus at the time of the formation of 
Kupffer's Vesicle (figure 12) pointed to an obvious relation between this vesicle and 
the supposed invagination. In a paper before the New York Academy of Sciences 
('99, b) I advocated a view similar to that of KOWALEWSKI. As then stated, my 
opinion was somewhat different from that presented in the present paper. 

The chain of evidence was not complete, however, until a form had been found 




Pr 



Section showing thickening of 
superficial layer (Pr) on pos- 
terior margin of early Amiurus 
blastoderm. 



SUMNER: KUPFFER'S VESICLE 



55 



FIGURE 12. 



which showed an open invagination in place of the solid ingrowth. The hypo- 
thetical primitive condition was shown, with diagrammatic distinctness, in the egg 
of an unknown eel (Mttraenaf), which it was my good fortune to secure, while at 
Naples, during the summer of 1899. 1 

Here, as in the preceding forms, the process is 
initiated by a thickening and indentation of the 
superficial layer on the embryonic side of the blast- 
oderm margin (figure 13). The next ensuing stage 
is represented in figure 14, which demands little ex- 
planation. Figure 13 represents the condition 
when the blastopore is still large, while in the next 
stage only a slender yolk-plug remains. The Vesicle 
of Kupffer has meanwhile attained a considerable 
size, so that it is readily visible in the living egg. 
In the latter there is also to be seen a fine canal con- 
necting the vesicle with the exterior, through the blastopore, and passing just in front 
of the yolk-plug. This condition is quite enduring, lasting for perhaps an hour after 
the appearance of the vesicle to view in the living eggi Sagittal sections removed 
any possible doubt as to the meaning of -this appearance. Here, as in the forms 

FIGURE 13. 




Posterior eiid of very early Amiurus em- 
bryo (cam. lac.) showing interesting rela- 
tion between superficial layer of the epiblast 
aud the vesicle. 




Sagittal section through blastopore region of early Murxnaf embryo (cam. Inc.). 

previously discussed, there is a direct continuity between the invaginated cell-layer 
and the lowermost layer of the embryo. 

Previous Observations of a Similar Nature. It is interesting to note that 
this manner of formation of Kupffer's Vesicle through an invagination from above 
is identical with that described by KUPFFER himself in 1879. The latter author 
came to this conclusion from the study of the living eggs of Osmems (see figure 15) 

1 In a paper read before the last session of the American Morphological Society ( New Haven, December 27, 
1899), I described this case, and referred to the egg as being that of Murasna. I have later learned from Dr. LoBianco 
that he is uncertain of the genus. 



56 



MEMOIRS OF THE NEW YORK ACADEMY OF SCIENCES 



and the pike (later also of the trout). 1 KUPFFER says that he confirmed these obser 
vations by sections, though none are figured. Curiously enough KUPFFER'S state- 
ments on this subject have been discredited by the majority of later investigators, 
though they have been confirmed by a few. Professor DOHRN informs me that he 
years ago noted the open connection in the case of the perch but had never pub- 



FlQUEE 14. 




FIGURE 15. 



EC 

Contiguous sections of Murxna t embryo cut as above somewhat later than preceding (camera lucida drawings). 
Pr, " prostoma " ; Yp, yolk-plug ; Kv, Kupffer's Vesicle ; GBy, gut-hypoblast ; NeHy, " non-embryonic " hypoblast ; 
EC, cells embedded in periblast. 

lished this observation. HENNEGUY ('88) noticed this condition in the case of the 
same fish but later concluded that he had been in error. He did not deny, how- 
ever, the possible accuracy of Kupffer's observation. RAFFAELLE ('88) observed in 
the living egg of Uranoscopus and in an unknown pelagic egg that KupfFer's Vesicle 
was for a brief period connected with the exterior through the blastopore. This 
connecting passage he regarded as equivalent to the neuren- 
teric canal. MC!NTOSH and PRINCE ('90) also speak of "what 
seems to be a tubular connection of the external blastopore and 
I pr the ventral surface of the embryo" but cannot be sure of an 
open passage into Kupffer's Vesicle. As none of these authors 
sectioned the eggs, we do not know whether the canals described 
had cellular walls or merely lay in the periblast. The "linear 
Optical section of egg of canal" mentioned by AGASSIZ and WHITMAN ('84) was evi- 
dently meYel y the disa PPearing blastopore. 

There is then every reason to believe that the open invagina- 
tion occurring in Murse.no, f and probably certain other fishes is represented by the 
ingrowing cell-mass found by Kowalewski in Carassius and Gobius and by myself in 
Amiurus, Noturus, Salvelinus, Fundulus and Ctenolabrus. The presumption is that the 
solid condition is the more frequent among the teleosts. This masked form of invagina- 

'The vesicle was first described by KUPFFER in 1866 in the embryos of Gasterosteus and Goliius. Its origin by in- 
vagination was not at that time maintained. SOBOTTA ('98) states that COSTE had figured the vesicle in 1847. LERE- 
BOULLET also ('63, figure 22) represents what appears to be Knpffer's Vesicle. 




(v- 
Of THE 
UNIVERSITY 



SUMNER: KUPFFER'S VESICLE 



57 



FIOUKE 16. 



SI 



tion is quite characteristic of the bony fishes, as witness the neural axis and auditory 
and optic vesicles. The later appearance of the cavity of Kupffer's Vesicle is 
strictly comparable with the delayed formation of the neural canal or the cavities of 
the sensory vesicles. 

It may be objected that the sort of Kupffer's Vesicle, described for Ctenolabrus 
and other pelagic eggs, in which a cellular floor is wanting, is not reducible to the 
above type. But as already stated, I find in Cteno- 
labrus a typical prostomal thickening, and I cannot 
regard the fact that these cells do not completely 
surround the cavity as of any importance to the 
theory. I shall later give reasons for believing 
that this condition is a retrograde one. 

Condition in Amia. Now is this condition in 
the teleosts a unique one or do we find a counter- 
part in any other group ? Dean has strongly main- 
tained that the key to teleost development is to be 
found among the ganoids, and from the generally 
accepted phylogenetic relation of the two groups, 
this proposition seems self-evident. 

It was DEAN'S endeavor (DEAN, '95, '96) to show 
that the ganoids, in their mode of development held 
a middle position between the elasmobranchs, on the 
one hand, and the teleosts on the other. My own 
observations as far as they go, sustain this view. As 
regards the special subject of the present paper, 
DEAN described in the eggs of Acipenser, Lepidosteus 
and Amia, what he considered to be the homologue 
of Kupffer's Vesicle in the teleosts. This was a 
cavity beneath and slightly anterior to the dorsal 
lip of the blastopore, bounded above and behind by 
the cells of the latter, below by the yolk. DEAN 
maintained that this cavity simply represented the 
angle formed by the blastoderm margin as it was 
mechanically turned in upon itself during its circumcrescence of the yolk. This 
simple mechanical explanation I cannot accept for the teleosts because (among other 
reasons) the vesicle in some fishes is not formed until the blastoderm has nearly or 
quite finished its journey over the yolk (Muravna ? Perca) and thus the supposed 




Blastopore region of Amia cut in sagittal 
plane (slightly schematized from camera 
lucida drawing). Yc, yolk cells; Sy, yolk 
syncytium; TV, " prostoraa " ; 81, superfi- 
cial layer of epiblast. 



58 



MEMOIRS OF THE NEW YORK ACADEMY OF SCIENCES 



mechanical cause no longer exists. But I believe that the homology proposed by 
DEAN is well founded. Through Professor DEAN'S kindness, I have had the privilege 
of studying some fine Amia material, and have found therein the counterpart of the 
phenomena just described for the teleosts. 

Figure 16 shows a longitudinal (nearly or quite sagittal) section through the 
egg of Amia at a time when the yolk-cells are perhaps three-quarters covered. The 
superficial layer of the epiblast is seen to be much thicker at the blastopore margin 
than elsewhere and to be directly continuous, around the margin, with the inner- 
most germ-layer. This condition occurs upon the entire periphery, but on the dor- 
sal (embryonic) side of the blastopore the case is somewhat more complicated. 
Here we find an arrangement strictly comparable to that found in figure 13. In 
both there is an enormous development of the pavement layer at the caudal end of 

the embryo and an indentation of its 

FIGURE 17. 

p Q 



N 6 H V 

1 *. 




Sy GHy'" *^ 

Blastopore region of Amia after closure (drawn as preceding). 
Up, blastopore; Ne Hy, "non-embryonic" hypoblast; GHy, 
gut-liypoblast ; Kv, probable homologue of Kupffer's Vesicle ; 
Sy, rudimentary syncytium. 



surface around which the cells are 
arranged in radial manner. In one 

O 

respect, hoAvever, this Amia embryo 
exhibits a more advanced condition 
than that of the Murasnaf embryo 
shown in figure 13. The notch in 
the pavement layer is seen to be con- 
tinued cephalad into a very notice- 
able cleft, extending forward for some 
distance into the hypoblast and sep- 
arating it into two layers. 



"Again the similarity between figures 17 and 14 is evident. The chief difference 
between the two seems to be that in the former there is wanting the open canal, con- 
necting the cavity of the vesicle with the exterior which occurs in the latter. This 
open canal is wanting even in embryos slightly younger than that shown in figure 
17, but the obliteration of its lumen is surely a matter of secondary importance. 
The ganoidean homologue of Kupffer's Vesicle, like that of many teleosts, has 
a ventral wall. This is well developed, though not quite complete, consisting of a 
layer of pavement-like cells, lying directly upon the large yolk cells. This condi- 
tion has not been mentioned by either DEAN ('96) or SOBOTTA ('96). 




SUMNER: KTJPFFER'S VESICLE 59 



III. RELATION OF PROSTOMA TO GERM LAYERS. 

Prostomal Thickening and Gut-Hypoblast. The exact part played by the 
prostomal cells in the formation of the germ-layers is very difficult to determine and 
in spite of a painstaking study upon a great many embryos, I cannot regard my 
observations on this point as quite conclusive. As already shown (figures 6 and 
13) in sagittal sections at a certain period, a direct continuity is observable between 
what I have called the " prostomal thickening " and a thin layer of cells which evi- 
dently represents the gut-hypoblast. This continuity is strong evidence that the 
latter has been derived by proliferation from the former. In the trout the pros- 
tomal invagination commences before the forma- 
tion of the germ ring. In Mursenaf on the other FIGURE is. 
hand, at a time when the blastoderm has covered 
nearly one-half the circumference of the yolk, 
neither prostomal invagination nor gut-hypoblast 
are to be seen, although the germ-ring is well 

Transverse section passing through posterior 

advanced. At the next Stage which I have Sec- eud of a very early trout emhryo (somewhat 
,. j ,n i n\ ii 11,- later than that in figure 6). PrHy, hypoblast 

tioned (figure 13) the gut-hypoblast is seen to be invaginated at ,, pro ; toma . 

in direct continuity with the invaginated cells, 

which agree witli the former in showing a higher staining power than the rest of 

the blastoderm. 

Transverse sections of these same stages unfortunately do not show these condi- 
tions with the same degree of clearness. 1 The lowermost cells do not exhibit the dis- 
tinct epithelial arrangement seen in longitudinal sections. In fact what appears in 
the latter as a continuous ventral layer, extending beneath the entire embryonic 
region of the blastoderm, is only to be observed with distinctness in those cross-sec- 
tions which pass through the extreme posterior end. It is clearly marked in several 
blastoderms which I have cut (figure 18). What becomes of this layer in the cepha- 
lad portion is not revealed in transverse sections of embryos at such an early stage. 
At a later period when the gut-hypoblast has finally become distinctly differentiated 
throughout the entire region of the embryo, it is found that a conspicuous break in 
its continuity occurs along a considerable extent of the axial line. The gut-hypo- 
blast now occurs in two lateral sheets, each sheet being merged axiad into the un- 
differentiated cord of cells which form the " primitive streak " (figure 19). 

The question at once arises was the lowermost germ-layer, at the time of its first 

1 I have transverse sections of Nolurus, Amiurus and Salvelinun embryos during this period, but unfortunately, none 
of Slnrtsna? 



60 



MEMOIRS OF THE NEW YORK ACADEMY OP SCIENCES 



FIGUBE 19. 




Transverse section through "primitive streak" 
region of a Noturus embryo of about the stage rep- 
resented in Figure 22. 



appearance, a single continuous sheet, which later fused along the axial line with 
the overlying cells; or was it differentiated from the overlying cell-layer, retain- 
ing, however, its original continuity with the latter along the axial line ; or finally, 
has the course of events been complicated by some process which we have left out 
of consideration ? So far, my study of sections has not afforded a final answer to 

this question. In longitudinal sections, such 
as those shown in figures 6 and 13, I can- 
not find any evidence of a break in the con- 
tinuity of this layer across the median line. 
Since, however, the layer is not perfectly 
distinct at all points, I cannot feel sure of 
this fact. As stated above, transverse sec- 
tions have so far failed to clear up the 
matter. 

The relation of the gut epithelium to the notochord is also an interesting 
problem. For some time after the chorda has separated from the neural axis above 
and the mesoblastic plates on each side, there persists a continuity between it and 
the gut-hypoblast. Does this continuity reveal the actual method of chorda forma- 
tion in teleost ontogeny, or is the union a secondary one ? Also, what is the mean- 
ing of the suggestive union occurring at certain points between the mesoblastic 
plates and the hypoblast? (See figure 20.) Clearer light may be thrown upon 
some of these points in the ensuing 
discussion of the meaning of the 
"prostoma." 

Before leaving this topic it is 
well to emphasize, however, that a 
part, though at present an uncertain 
part, of the embryonic hypoblast is 
derived from that collection of cells 
which I have called the prostomal 
thickening. Whether or not this 
mode of origin is supplemented by 

differentiation from the overlying "secondary layer" of the germ-ring, I cannot 
definitely say. 

There is certainly one region of the blastoderm where the hypoblast originates 
quite independently of any connection with the prostomal invagination, namely, 
the "non-embryonic " part of the germ-ring. Shortly before the blastopore closes, 



FIGURE 20. 




Section passing through posterior half of embryo of Noturus, 
somewhat in front of Kupffer's Vesicle (cam. luc. ). The relations 
of gut-hypoblast to mesoblast and chorda are suggestive. 



SUMNER: KUPFFER'S VESICLE 61 

that part of the blastoderm forming its now posterior margin (in Noturus and 
Murxnaf) is seen to be differentiated into all three germ-layers (see figure 14). 
VIRCHOW ('95) describes this condition in the trout and I ('99, a) have already re- 
corded it for Noturus. 

Historical. With the exception of the earlier investigators, who derived the 
entoderm from the periblast, nearly all writers on fish embryology have thought 
this germ-layer to arise as a differentiation from the inflected layer of the germ- 
ring. The cells of the latter were held to separate sooner or later into two layers, 
the lower of which was the entoderm, the upper the mesoderm. The only writers, 
as far as I know, who have maintained the existence of a distinct entoderm rudi- 
ment are KOWALEWSKI (see above), BERENT ('96), and REINHARD ('98). 

BERENT maintains that the entoderm arises as a separate rudiment and has 
figured it with approximate correctness in one stage (figure 21, B). But his inter- 
pretation of the process is certainly wrong. The accompanying figure 21, A from 

FIGURE 21. 





After Berent illustrating his view of entoderm formation. 

BERENT is probably based upon an observation, though an inaccurate one, of the 
earlier appearance of this same group of cells. He holds the condition described 
by H. V. WILSON ('91) for the sea-bass (i. e., a uniform differentiation of the under 
surface of the whole embryonic germ-ring) to be the more primitive, and considers 
the condition he finds in the trout to be a derived one. My objections to BERENT'S 
conclusions are two : first, that the rapidly developing pelagic egg of the sea-bass 
would be far more apt to exhibit a precocious development of any part than the 
slowly developing egg of the trout, and second, that the account offered by WILSON is 
undoubtedly incomplete. While I have never studied the egg of the sea-bass, I 
have carefully sectioned the appropriate stages of the quite similar egg of Ctenola- 
brus, and find that here, as in the eggs of so many widely different fishes, the gut- 
hypoblast is plainly formed in connection with a prostomal ingrowth. Unlike 
KOWALEWSKI, BERENT has missed the true meaning of the process. REINHARD re- 
gards the entire hypoblast as derived by proliferation from the walls of Kupffer's 
Vesicle. The cells of the latter arise, he claims, from the periblast. 



G2 



MEMOIRS OF THE NEW YORK ACADEMY OF SCIENCES 



IV. SIGNIFICANCE OF THE PROSTOMAL INV AGINATION. 

I now purpose to offer an interpretation of the foregoing phenomena and to 
point out their relation to phases in the development of other vertebrates. 

My theory in large degree reverts to that proposed by KUPFFER in 1884. (Prac- 
tically formulated in 1879 see KUPFFER, '79.) KUPFFER'S observations, already 
discussed, led him to a novel view of the development of the fish embryo. It 
was his endeavor to make a complete comparison between the conditions found 
in the Teleostei and that found in the Amniota. KUPFFER was the first to point 
out the dorsal invagination in the reptilian blastoderm, and he considered that 
in this he had found the homologue of the gastrula mouth or blastopore of 



FIOUBE 22. 



FIGURE 23. 





Pr- 



Early blastoderm of Noturus. Gr, germ- 
ring ; Pr, "prostoma." 



Early embryo of Notaries, before appearance of 
caudal knob. The bifid caudal end of the embryo 
si seen to embrace a thin cellular sheet as in case 
of Murxna ? (see figure 27). 



Amphioxus. The yolk-filled aperture of the blastoderm margin had nothing to 
do with blastopore proper. For this aperture he invented the name of " BLAS- 
TOTREMA." He considered the process by which the blastoderm covered the yolk 
as in no sense a process of gastrulation but as a process of blastosphere formation, 
thinking that the completed blastosphere would surround the yolk on all sides. 
KUPFFER sought for and found a homologue of the reptilian prostoma in teleosts. 
This invagination, he says, at first + shaped in . surface view, greatly elongates 
in a forward direction, resulting in the formation of a longitudinal groove which he 
calls the " primitive groove," extending along the axis of the embryo. He dif- 
fers in his interpretation of the primitive groove and streak from BALFOUR, who, 
as well known, considered these structures to be homologous with the seam along 
with the blastoderm edges united behind the embryo in the elasmobranch (figure 
29, A, Bl). Thus, according to BALFOUR ('78 and '81) the primitive streak is posterior 




SUMNER: KUPFFER'S VESICLE 63 

to the neurenteric canal, while KUPFFER held it to be anterior to the latter. The 
real representative of the " Raphe" or line of union of the blastoderm edges of the 
shark, KUPFFER found in the so-called " Caudal Knob" (Randhugel) appearing at 
an early period .at the posterior end of the trout embryo. This comparison was 
based upon the supposition that the " prostoma," or blastopore of the meroblastic 
vertebrates, was originally situated upon the blastoderm margin as in the shark, and 
that its more anterior position in Teleosts and Amniota had been due to a removal 
from its primitive situation. It is not plain, however, whether or not KUPFFER 
regarded this change of position as occurring in the ontogeny of the teleosts. 

This original invagination, occurring immediately in front of the caudal knob, 
resulted in the formation of the much discussed vesicle, which represented that part 
of the archenteron forming the allantois of the amniota. 

There seems to be no doubt now that the " primitive FIGURE 24. 
groove " seen by KUPFFER in the trout was nothing more than 
the medullary furrow. KUPFFER apparently made little use of 
sections and he does not give a single figure of one illustrating jfcj 
this point. But his main ideas, namely, that there occurs in 
the teleosts a dorsal invagination comparable to that in the rep- 
tile, and that this invagination has been removed from a prim- '-^^..^f^ ^" ~7 

itive marginal position, are fully borne out by the present in- 
vestigations. I differ from KUPFFER, however, in regarding the Not s embryo after ap- 
pearance of caudal knob 
"prostoma" as representing only a limited part of the blasto- (<*). 

pore, the remainder being constituted by the entire blastoderm 
margin (KUPFFER'S " Blastotrema"). Again I find strong evidence that this detach- 
ment of the "prostoma" from the margin occurs in ontogeny in the teleost as well 
as in the elasmobranch, a point left uncertain by KUPFFER. 

If such a process occurs, it must on theory take place some time before the ap- 
pearance of Kupffer's Vesicle, since this represents the expanded inner end of the 
" prostoma." Figure 22 shows the condition of a Notunw blastoderm at this period. 
The indentation of the blastoderm margin and its relation to the future axis of the 
embryo recall at once the figures, given us by Duval ('84) of the early chick blasto- 
derm. These will be referred to "later. Figure 23 represents a somewhat more ad- 
vanced stage and demands no further explanation. Figure 24 shows the appear- 
ance of an embryo in which Kupffer's Vesicle has formed. The indented posterior 
margin has now given place to a rounded projection, a condition which we should 
expect to exist after the final union of the sides of the indentation, in other words, 
after the detachment of the prostoma from the yolk blastopore. 



64 



MEMOIRS OF THE NEW YORK ACADEMY OF SCIENCES 



The emarginate condition is also well shown in the transparent living egg of 
Scorpsena. It is true that figure 25 shows a shallow bay rather than sharp nick, 
but the conspicuous shingle-like overlapping of the cells along the whole posterior 
margin speaks strongly for a process of concrescence. This appearance of the blas- 
toderm margin recalls the descriptions of RYDER ('86) for Elacate and of LOCY ('95) 
for Squalus, and it seems quite possible that the structures which were by those 
writers given a segmental value find their true explanation here. In figure 26 
the emarginate condition has for some time ceased to exist and Kupffer's Vesicle 
has come into view at a considerable distance from the margin. Behind this the 
caudal knob projects into the yolk blastopore. 

In the egg of Mursena f which, as above described, exhibits the prostoma in its 
least modified form, there is also the strongest evidence of this view of its formation. 



FIGURE 25. 



FIGURE 26. 





Pr' 



Living egg of Scorpsena. Pr, emarginate region at pos- 
terior end (not often as deeply concave as in figure). Ar- 
rangement of cells on either side strongly suggests con- 
crescence. 



Kv 



Scorpiena embryo after caudal knob and Kupffer's Vesi- 
cle have appeared to view. 



Figure 27 shows a deep bay extending forward from the blastopore into the pos- 
terior end of the embryo. It is evident from transparent view that the yolk is not, 
however, exposed in this bay as in the rest of the blastopore (compare with condi- 
tion in Noturus, shown in figure 23). The section (figure 28) verifies this opin- 
ion. It is at once seen that at this point the invagi nation to form the hypoblast (as 
above discussed) is occurring. 

In this connection it is significant to note that* there seems to be a certain rela- 
tion between the time of appearance of the caudal knob and the age at which Kupf- 
fer's Vesicle is formed. In the embryo of the trout, the caudal knob appears at a 
period when the blastoderm occupies but a small part of the upper hemisphere of 
the egg. The definitive Kupffer's Vesicle is formed some time before the equator 
has been passed. In Noturus, the caudal knob is not developed until about one-half 



SUMNER: KUPFFER'S VESICLE 65 

of the yolk has been covered, and Kupffer's Vesicle appears somewhat later ; in Scor- 
pxna, the relative time of formation of both of the structures is still later, at least 
four-fifths of the yolk being covered ; while in Mursena f the vesicle does not ap- 
pear to view until the blastopore is nearly closed, and the caudal end of the embryo 
is conspicuously bifid up to the time when the blastopore is very small. Of course 
this line of argument is open to the reply that the time of appearance of both these 
structures is conditioned by the general rate of development of the embryo in any 
particular case and that the coincidence stated does not prove any necessary relation- 
ship between the two. 

As might be expected, an indentation of the posterior end of the embryo 
has been already noted by several investigators. AGASSIZ and WHITMAN ('84) once 
or twice observed this condition in living pelagic eggs and regarded it as strong evi- 
dence for the formation of the embryo by concrescence. HENNEGUY ('88) noted its 

FIGURE 27. FIGURE 28. 





The posterior end of embryo of Marietta? just prior to Oblique section of preceding embryo, 
closure of blastopore. Yp, yolk-plug ; Pr, " prostoma " ; 
X-Y, plane of section shown in FIGURE 28. 

existence in a single egg and from this concluded that concrescence occurred in the 
fish embryo in a very limited region, i. e., enough to form the caudal knob, although 
not giving to this process any such interpretation as we have had under discussion. 
M'INTOSH and PRINCE ('90) speak of a " terminal bay " in certain pelagic eggs. 
EYCLESHYMER ('95) notes that this indented condition has been already observed in 
Amiurus by Miss O'GRADY, of Vassar. JABLONOWSKI ('98) describes the artificial 
production of a similar condition in the Salmonidse, by the use of salt solution. 

Reference might also be made to the Mesodidymi arid Hemididymi of various 
authors, although I do not consider it necessary to discuss them here. 

Thus the prostoma of the teleost, like the neurenteric canal of the shark, repre- 
sents a specialized portion of the blastopore which has become detached from the 
remainder by a process of concrescence or union of the blastopore lips. Figure 29, 
A and B, illustrate this comparison. It will be seen that the homology suggested 



66 



MEMOIRS OF THE NEW YORK ACADEMY OF SCIENCES 



by KUPFFER between the caudal knob (Ck) of the teleost and line of fusion of the 
blastoderm edges behind the embryo of the shark is in principle true, though not 
quite exact. The caudal knob of the teleost represents, rather, the embryon'c tail- 
end of the shark, enclosing the neurenteric canal. (This is the view maintained 
by SCHWARTZ '89, though I did not know it when the foregoing words were written. 
Concerning KOPSCH, see below.) The posterior line of fusion (Bl) has, strictly speak- 
ing, no counterpart in the teleost, inasmuch as the embryo retains its connection 
with the blastoderm margin. 

H. VIRCHOW ('95) proposes a view of the teleost embryo similar in some respects 
to those of KUPFFER and myself. He considers the caudal knob to result from a 
process of folding similar to that in the elasmobranch. In the latter, he says, all 
three of the germ layers are folded off and the tail projects freely, while in the trout 
the folding process affects merely the ento- and mesoderm, the ectoderm taking no 

FIGURE 29. 





Schematic figures of A, early elasmobranch (after Balfour), and B, teleost embryo illustrating the author's view of 
the formation of the teleost embryo. Mg, medullary groove ; Ne, neurenteric canal ; Bl, line of fusion of edges of 
blastoderm behind the latter ; Yk, yolk. 

part in the process. This ventral folding off of the entoderm results in the forma- 
tion of Kupffer's Vesicle which is thus, in its origin, like any other part of the gut. 
But as VIRCHOW does not recognize the presence of the " prostoma," nor the part 
played by concrescence, his account is incomplete. He attributes a similar view to 
OELLACHER, though I do not recall the latter's statement. The views of KOPSCH 
and JABLONOWSKI will be considered below. 

If the theory I have advocated is correct, it is evident that the development of 
the teleost egg differs far less from that of the other meroblastic vertebrate eggs than 
has usually been held. In all of these, it seems probable that the originally simple 
blastopore, consisting of the whole exposed surface of the yolk, has been separated 
by a process of concrescence into an anterior, embryonic portion and a posterior, non- 
embryonic portion. For the bird's egg, the case has been convincingly presented by 
DUVAL ('84). The primitive streak, although appearing to originate at some distance 



SUMNER : KUPFFER'S VESICLE 67 

from the blastoderm margin, has been shown by him to arise in connection with the 
latter, in fact, to arise from a portion of the latter. 1 In the egg of the reptile, the 
connection of the plate of cells in which invagination occurs with the blastoderm has 
not yet been established, but we cannot doubt that even here the invaginate part has, 
in phylogeny at least, been detached from a primitive position on the margin. An 
interesting parallel occurs between the fate of the prostomal cavity (KupfFer's Vesicle) 
in the teleost and that of the invaginate cavity in the reptile. It has been shown 
that the latter breaks through at its inner extremity, thus becoming connected with 
a large sub-germinal cavity. Two regions of the archenteron, at first separate, are 
thus brought into union. Now SOBOTTA ('98) at least I so interpret Sobotta's state- 
ment and GREGORY ('99) have described a union, at a certain period, between the 
cavity of KupfFer's Vesicle and the lumen of the gut in front of it. This parallel 
was called to my attention by Professor MINOT. 






A, B and C. Showing three stages in the formation of the embryo of the toad-fish. (After Miss Clapp. ) 

This dichotomizing of the blastopore is, in the elasmobranchs and the sauropsida, 
very evidently due to the great relative amount of the yolk, which renders the epi- 
bolic growth of the blastoderm a slow process, thus making it necessary for the form 
of the embryo to be established long before the close of gastrulation. In the case 
of the teleost, the yolk as a rule is far smaller, so that the yolk blastopore is able to 
close at a period when the embryo is much less advanced. In this group, accord- 
ingly, we find the caudad growth of the embryo to take place at a rate about equal 
to that of the blastoderm margin. Consequently, although the folding-off of the tail 
end occurs here, as in the shark, resulting in the detachment of a portion of the blasto- 
pore, the embryo retains throughout its connection with the border of the blastoderm. 
That this is the true explanation of the difference in the position in the two groups 
is shown by certain exceptional teleosts where this marginal position of the embryo 

1 It is true that the experiments of ASSHETON ('96) have not supported Duval's theory. [Since writing the fore- 
going, I have fully confirmed such of Assheton's results as go to prove that the primitive streak of the bird does not 
arise from the blastoderm border in ontogeny. This in no way disproves, however, that it so arose in phytogeny, and 
I believe that there still remain strong reasons for such a view. September 21, 1900.] 



68 MEMOIRS OF THE NEW YORK ACADEMY OF SCIENCES 

is lost. In the toad-fish (Batrachus) according to Miss CLAPP ('91) the caudal end 
of the embryo parts company with the margin of the blastoderm at a relatively 
early period (figure 30, A, B, C). EYCLESHYMER reports a similar condition for 
Lophius, though his description and figures do not bear out such a contention. The 
latter author infers from certain appearances that the same process occurs in Amiurus 
as well. In this, EYCLESHYMER is certainly wrong. The embryo of this fish in- 
variably retains its connection with the germ-ring until the complete closure of the 
yolk blastopore. JHERING, also, ('88) has described what seems to be a similar 
condition for the South American cat-fish Arius. The eggs of Batrachus, Lophius and 
Anus are all extraordinarily large, the latter being as much as 18 mm. in diameter. 
Again, CORNING ('96) reports this separation of the caudal end of the embryo from 
the germ-ring to exceptionally occur at a relatively late period in the salmon. 

It will be recalled that Amia was included among the forms exhibiting the "pro- 
stoma," although it is now certain that the egg of Amia is a holoblastic one. How- 
ever this may be, the egg of this ganoid is probably secondarily holoblastic, for it 
shows other evidences than the one considered of having been derived from a mero- 
blastic egg. For instance, my discovery in Amia (SUMNER, '00, a) of a rudimentary 
syncytium or "periblast" with typical giant nuclei confirms the above view (figures 
16 and 17) although this fact is also open to the interpretation, as Professor Mi not 
has suggested to me, of being anticipative of the condition found in the teleost rather 
than derived from that in the shark. I do not, however, offer the case of Amia as 
being nearly as well established as that of the teleosts mentioned. 



V. CONCRESCENCE AND CONFLUENCE. 

Problem Stated. In the foregoing pages concrescence has been discussed only 
in relation to that process by which the hinder end of the embryo becomes folded 
off from the margin of the blastoderm. It will be remembered that BALFOUR ('81), 
while admitting the occurrence of concrescence to this extent, denied to it any part 
in the formation of the embryonic body itself. So far, I have affirmed the process 
only to the degree admitted by BALFOUR. 

I cannot go further, however, without facing the general problem of concrescence. 
I must defer to a subsequent paper a review of the endless mass of literature bearing 
upon this interesting subject. In this article, I shall merely point out that my conclu- 
sions are quite in harmony with the results reached by the two investigators who have 
given most labor to the experimental study of concrescence in the teleost embryo. 



SUMNER: KUPFFER'S VESICLE 69 

" CONCRESCENCE " as applied to the growth of the embryo, may be taken in two 
entirely different senses and a failure to recognize this difference may lead to 
much confusion. As originally conceived by His, 1 concrescence was a process by 
which the lateral portions of the germ-ring were actually apposed to one other be- 
hind the embryo, which thus grew backward pari passu with their union. In this 
case, the embryo was looked upon as formed by the coming together of the two 
halves of the germ-ring as such, the successive levels in the body of the former 
being at the outset represented by successively distant portions of the circumference 
of the latter. The term " concrescence " may be extended in its application, how- 
ever, to a process quite different from this. The growth of the embryo may still 
be regarded as taking place at the expense of the germ-ring, but merely in the sense 
that the latter furnishes undifferentiated building-material, which is first organized 
after reaching the embryonic region. In this case, the cells composing the opposite 
halves of the germ-ring are conceived to undergo a gradual concentration toward the 
axis of the embryo as the latter grows backward. This axial concentration is part 
of the same general process by which the broad "embryonic shield" gives rise to the 
narrow, but greatly thickened body of the definitive embryo. It is clear that such a 
centripetal movement of the germ-ring cells would not necessarily result in a growth 
of the embryo by accretion at its posterior end. On the contrary, its growth might, 
in this case, occur by intussusception, the newly added cells reaching the embryo at 
some point anterior to the caudal end. This second possible mode of concrescence 
I prefer to call " CONFLUENCE." 

Turning to my own studies, it is evident that I have explained the detachment 
of the prostoma from the yolk blastopore by a process of concrescence in the former 
sense (apposition). The duration and extent of this process I have not so far de- 
termined, but for many reasons I feel convinced that the folding-off of the hinder 
end of the body is merely the last step in a process of concrescence by which a whole 
or part of the length of the then-existing embryo has been formed. I am equally 
convinced that after the detachment of the prostoma and the appearance of caudal 
knob, concrescence in this sense ceases entirely. For unless we believe this to have 
come to a close, it would be difficult to account for the continued presence of the 
projecting caudal knob at the posterior end. Again, if there occurred a union of 
the halves of the germ-ring behind the embryo, after the formation of Kupffer's 

1 First stated in print in Verh. d. Leipzigcr naturfor.ich. Gen., June 5, 1874. In the same year appeared " Unsere 
KiJrperform." After this, His reiterated the theo y many times, his last utterance on the subject being in 1891 (see 
His '91). His was in large measure anticipated by LKUEBOULLET ('63) who distinctly affirmed that the two sides of 
the embryonic body arose from the two halves of the " bourrelet blaslodermique," or "bourrelet embryngene," as he calls it 
in view of its supposed fate. Lereboullet did not, however, describe the procesa by which the body arose from the 
" bourrelet." 



70 MEMOIRS OF THE NEW YORK ACADEMY OF SCIENCES 

Vesicle, there would be a rapid increase in the distance between the latter and the 
caudal end. But this, as HENNEGUY was first to point out is untrue. The writer 
has verified HENNEGUY'S observations, which were made upon trout embryos, by 
camera lucida measurements made upon the living egg of Scorpsena. Whether, 
however, there occurs, after the appearance of the caudal knob, a process of con- 
crescence in the second sense ("confluence") is a question upon which my own 
observations throw little light. [See Supplement.] The experiments of other in- 
vestigators, as we shall see, speak strongly for this view. 

Experiments of Morgan and Kopsch. MORGAN ('95), l working upon the egg 
of Fundidus, performed the crucial experiment of cutting the germ-ring to one side 
of the embryo. In a number of cases, nearly normal embryos developed in spite of 
their severance from the hypothetical sources of building material. He rejects, on 
what seems to me to be insufficient grounds, the supposition that a process of regen- 
eration has taken place on the injured side. MORGAN'S accounts, it is important to 
note, mentions two cases in which injury to the germ-ring resulted in a deficiency of 
mesoblast on tbe corresponding side of the embryo. For this and other reasons he 
concludes that the germ-ring normally does, in part at least, pass into the embryo 
during growth, but he also holds that it furnishes a relatively small part of the sub- 
stance of the latter. 

The experiments conducted by KOPSCH ('96) 2 upon the egg of the trout differ 
from those of his predecessor mainly in being more systematic and thorough. He 
finds that the effects of injuring the germ-ring vary with the position of the injury 
and the stage at which it is inflicted. After the caudal knob has appeared, any in- 
jury to the germ-ring laterad to this does not prevent the corresponding side of the 
embryo from developing with a normal number of somites. This side is, however, 
less strongly developed, in point of quantity of mesoblast, than the opposite (thus 
agreeing with MORGAN). Injury to the embryo during an early " embryonic shield" 
stage, before a tail-bud has appeared, gives results varying according to the location 
of the injury. If the latter is in the center, no growth takes place here, but the 
"non-embryonic" borders of the blastoderm continue to surround the yolk, leaving 
the punctured spot, at the time of closure, at the (anterior) end of a long slit. If 
the injury is slightly laterad, an embryo develops, having a perfect head, but the 
trunk fails to develop on the injured side. If the injury is still further laterad, an 
embryo results but not so strongly developed on the injured side (see above). 

1 A preliminary account of these experiments is to be found in the Aunt. Anz., 1893. KASTSCHKNKO, in 1888, 
had performed similar experiments upon the Selachian embryo, his results leading him to oppose the concrescence theory. 

2 KopSCH's later experiments ('99) in producing artificial Hemididymi I shall not discuss, inasmuch as his results 
are equally well explained upon the orthodox view of concrescence. 



SUMNER: KUPFFER'S VESICLE 71 

KOPSCH concludes from these results that, at the time of the first appearance of 
the embryonic Anlage, the head rudiment (K, figure 31, A) is median, the cell- 
masses (R) destined for the halves of the trunk and the tail are laterad to this. The 
formation of the caudal knob ("Knopf," R, figure 31, B) results from the union 
of the two lateral masses behind the head rudiment. The " Knopf" is the re- 
pository for the trunk and tail Anlagen, which thus, even at this early stage, 
come to lie in the middle line. The caudal knob does not, however, depend for its 
growth entirely upon a multiplication of its own cells but, during the circumcres" 
cence of the germ-ring, receives successive portions of the latter, which serves merely 
as so much undifferentiated building material. A concrescence in the sense em- 
ployed by His, he says, does not occur in the Salmonidne. The process by which the 
two rudiments (R) came together KOPSCH does not consider as concrescence, although 
he does not give sufficient reason for his position. He makes the very significant 

FIGURE 31. 





Diagrams illustrating Kopsch's view of the formation of the embryo from the germ-riug. K, head anlage. 

R, material destined to form the trunk. 

remark that the Knopf " contains the neurenteric canal," although not stating just 
what he means by this. In a later paper ('99), he says that the Knopf is " com- 
posed of two symmetrical halves which are separated from one another by the ideal 
neurenteric canal." 

The above conclusions of MORGAN and KOPSCH are in no way inconsistent with 
the results of HENNEGUY'S measurements. HENNEGUY located the growing region 
of the embryo between Kupffer's Vesicle and the most recently formed somite. 
This HENNEGUY offered as convincing evidence against concrescence by apposition, 
and in doing this he seems to have struck the first decisive blow against His's the- 
ory. If concrescence (confluence) is occurring at all, he says, it occurs in front of 
Kupffer's Vesicle. This supposition, however, he also rejects for reasons which I 
shall not here discuss. It will be remembered that HENNEGUY believed the caudal 
knob (and this only) to be formed through an actual process of concrescence proper. 



72 MEMOIRS OF THE NEW YORK ACADEMY OF SCIENCES 

There is no evidence, however, that HENNEGUY attributed any such significance to 
this last phenomena as has been done by KOPSCH and myself. 

JABLONOWSKI ('98) offers a view based largely upon that of KOPSCH, though not 
identical with it. He regards the first formed section of the embryo as resulting 
from an " excentrischer Zusammenziehung " of the blastopore lips, comparable to 
that described for Amphioxus by HATSCHEK. After the definite establishment 
of the embryonic body (he seems to have in mind the appearance of the caudal 
knob) growth occurs "through multiplication of material situated in the Endwulst." 
The latter represents only the anterior (dorsal) wall of the neurenteric canal, which 
is thus, properly speaking, an "incisura neurenterica" (His) like that of the early 
elasmobranch. In favor of this view he cites the artificially produced bifid condi- 
tion above described. JABLONOWSKI, accordingly, recognizes no detachment of the 
neurenteric canal from the rest of blastopore. Neither JABLONOWSKI nor KOPSCH 
hint at any relation between neurenteric canal and Kupffer's Vesicle. 

Conclusions. From the previous discusion, it is evident that I have been led, 
on purely morphological grounds, to a view of the formation of the fish embryo in 
full accord with the results of the latest work in the experimental field. The neur- 
enteric canal I have shown, moreover, to be much more than an "ideal" structure, 
it having, in some cases at least, an open lumen, and being in others represented 
by a solid ingrowth of cells. Of course, such a neurenteric canal as occurs in Am- 
phioxus is impossible in any teleost, owing to the solid condition of the neural axis. 

That the process cf concrescence, which leads to the formation of the neurenteric 
canal, was previously instrumental in the construction of the embryonic body an- 
terior to it seems to be proven by KOPSCH'S experiments, although he rejects the 
word "concrescence" in this connection. He admits, however, an apposition of the 
two laterally situated halves, which is all that is necessary for the present discussion. 

The long-continued emarginate condition at the caudal end of certain embryos 
(see above) leads me to the belief that the process of concrescence is one of con- 
siderable duration, and the fusion of the germ-layers along the embryonic axis 
("primitive streak") in front of this point, gives further evidence of such a process. 
(JABLONOWSKI also regards this region as due to "Nahtbildung.") That concrescence 
(apposition) ceases, however, with the formation of the neurentoric canal is certain. 
That it is thereafter replaced by a process of confluence seems proven by the experi- 
ments of both Morgan and Kopsch. [Also by my own. See Supplement.] 



SUMMER : KUPFFER'S VESICLE 73 



VI. FURTHER MORPHOLOGICAL CONSIDERATIONS. 

The generally accepted view that Kupffer's Vesicle represents a certain part of the 
archenteron seems to me to be true beyond doubt. How much of the gastrula cav- 
ity is represented by this structure is less obvious. It has been variously inter- 
preted as the allantois, as part or whole of the post-anal-gut, or as the latter plus the 
neurenteric canal. The case of Murasna f seems to show that the neurenteric canal 
is not included in the vesicle proper, but that the latter forms the dilated inner end 
of the imagination which gives rise to both. The neurenteric canal, in most teleosts, 
is represented by a solid ingrowth as maintained by KOWALEWSKI. At the inner 
end of this, the cavity of the vesicle forms secondarily. 

In many fishes, the post-anal portion of the gut FIGURE 32. 

(Kupffer's Vesicle) possesses from the first, as we 
have seen, a ventral as well as a dorsal wall. In 
the more anterior portion, or gut proper, the lumen 
is formed by a real or virtual upfolding of a hori- 
zontal sheet of cells, the axial portion of which rep- 
resents the dorsal wall of the gut, the lateral por- 

Sagittal section showing late Kupffer's 

tions representing the ventral. We should thus Vesicle in 




expect the gut-hypoblast to be continued into the 

dorsal, rather than the ventral wall of Kupffer's Vesicle. This relation is impossible 
to determine at an early period, but figure 32 representing a late stage of the vesicle 
in the trout embryo, exhibits the expected condition. 

The structure of the fully formed Kupffer's Vesicle and adjacent parts have been 
so often carefully described that they need not be discussed here except in connec- 
tion, with certain differences, already mentioned, in the form which it assumes in 
various types of fishes. Two different types of Kupffer's Vesicle have been de- 
scribed above, one with no cellular floor, the other from the first bounded on all 
sides by cells. That these two types exist there can no longer be any doubt. Ac- 
cording to the view maintained in this paper as to the significance of this structure, 
it is obvious that I must regard the second type as the more primitive. The forms 
in which the cellular lower wall has been described as wanting are, as far as I know, 
all rapidly developing pelagic eggs, in which many developmental processes are 
modified by abridgement. On the other hand the more slowly developing cat-fish, 
trout and salmon, in which there is reason to believe that the type of development 
is less modified, display a vesicle with a complete cellular boundary. An exception 



74 MEMOIRS OF THE NEW YORK ACADEMY OF SCIENCES 

to such a rule, however, is found in the case of Mursena ? a form having a rapidly 
developing pelagic egg, for this fish exhibits, in the formation of its vesicle, the 
hypothetical primitive condition more clearly than any other tcleost that has been 
carefully described. 

I have observed both in Mursena ? and in the trout an incompleteness of the 
lower wall of the vesicle at one period. The cells forming the floor of the vesicle 
are embedded in the underlying periblast (figures 13 and 14, A) and are not at 
all points in contact with one another. This fact, and the presence of free cells in 
the periblast in this neighborhood, might lead to the suspicion that the lower wall 
of the vesicle is formed by cells derived from the syncytium. In fact, this is a 
conclusion adopted by REINHARDT ('98). I do not find any evidence that these free 
cells are formed out of the periblast proper. They are present from a very early 
period in the development of Salvelinus, and occur beneath the whole blastoderm, 
but especially at the posterior end. It seems probable that their differentiation as 
cells dates back to the segmentation period. If so, they are to be regarded merely 
as detached segmentation spheres which have lost all connection with the blasto- 
derm. They are surrounded by distinct cell-walls and generally contain normal 
nuclei. Whether such free cells play any part in the building of Kupffer's Vesicle 
in Salvelinus or Mursena f I cannot say definitely. In Amiurus and Noturus such free 
cells are of very rare occurrence. I have observed them only once or twice in all the 
Amiurus eggs examined, and do not recall having seen any in the eggs of Noturus. 
OELLACHER ('72) was probably the first to describe this conditional though he un- 
doubtedly made no distinction between these embedded cells and the periblast nuclei. 

Such an incompleteness in the ventral wall may be looked upon as a condition 
transitional between the two types of vesicle described. SOBOTTA ('98) has already of- 
fered as a transitional form the case of the rainbow trout, which exhibits a complete, 
though extremely thin, lower wall. That such cases as I have described may have, 
however, some other morphological significance is suggested by the occurrence of a 
like condition in Amia. Here I find, in both transverse and longitudinal sections 
that a gap in the cellular floor of the vesicle occurs along the median line, the floor 
being completed by the syncytium (figure 17). 



VII. YOLK VESICLES. 

In certain fishes, there exists, in addition to Kupffer's Vesicle, a second vesicle 
lying in the yolk beneath the floor of the former. This structure is well shown in 



SUMNER: KUPFFER'S VESICLE 



75 



the egg of the " Stone Cat " (Notu.rus) although entirely lacking in the closely re- 
lated Amiurus. It appears a little earlier than Kupffer's Vesicle and attains a 
somewhat greater size (see figure 34). This " yolk vesicle " is surrounded by a com- 
plete wall of periblast, except on the upper side, where it is bounded directly by 
the cells of the embryo. There is at no time any connection between the two. 

EIGENMANN ('92) describes in the case of Cymatogasler a single large vesicle lying 
partly in the yolk and partly in the embryo. This latter subdivides into three por- 
tions : a lower one, which he calls the "yolk vesicle" ; an intermediate portion, 
which he homologizes with the post-anal-gut, and an upper cavity, which he con- 
siders to be the equivalent of the neurenteric canal. KINGSLEY and CONN ('83) orig- 
inally described Kupffer's Vesicle in Ctcnolabras as arising by the fusion of a mass 



FIGURE 33. 



FIGURE 34. 




Kv 




Section through posterior end of same embryo as that 
in figure 20 (camera lucida). Kupffer's Vesicle (Kv) is 
beginning to make its appearance aa a group of small 
vesicles. 



Transverse section through embryo of Noturus, showing 
fully developed Kupffer's Vesicle and yolk vesicle. A 
layer of pavement cells, continuous with the gut-hypo- 
blast, is here seen to underly the columnar epithelium 
forming the lower wall of Kupffer's Vesicle. This 
pavement layer has probably reached its present position 
by growing in from the sides, as it is not present in an 
earlier stage (figure 33). 



of vacuoles in the yolk, and^this account has been confirmed by several subsequent 
workers on pelagic eggs (e. g., AGASSIZ and WHITMAN, see p. 52, ante). The dorsal 
cellular wall of the vesicle becomes differentiated above this resulting single vacuole. 
HENNEGUY ('88) states that there frequently occurs beneath the posterior end of the 
trout embryo a. vesicle in the yolk. In the case of Salvelinus the whole periblast is 
much vacuolated, but in some specimens I have found a particularly large vacuole 
lying in the appropriate position. AGASSIZ and WHITMAN have described certain 
"secondary caudal vesicles" probably of a similar nature. M'!NTOSH and PRINCE 
('90) speak of a "multiplicity of vesicles" in the gurnard and some other fishes. 
EYCLESHYMER ('95) describes two such accessory vesicles in Lophius, one of which 
communicates at one period with Kupffer's Vesicle. 



76 MEMOIRS OF THE NEW YORK ACADEMY OF SCIENCES 

I am convinced, from the mode of formation of Kupffer's Vesicle, that it has no 
morphological relation to these lower cavities in the periblast. In such cases as 
those of Ctenolabrm the connection is doubtless a secondary one. But there prob- 
ably exists, as I maintain below, a physiological connection between the two. 



VIII. THE FUNCTION OF KUPFFER'S VESICLE. 

The .preceding pages have been largely concerned with a morphological interpre- 
tation of the embryonic structure known as Kupffer's Vesicle. It is obvious that 
this morphological interpretation in no way accounts for the dimensions attained by 
the vesicle during development. Why should the lumen of this quite transitory 
post-anal-gut reach such a relatively enormous size at a time when, in the remainder 
of the gut, no lumen has appeared? Kupffer's Vesicle must be regarded as an em- 
bryonic organ having some definite part to play in the economy of the growing em- 
bryo. The measurements made by Henneguy located the region of growth in the 
unsegmentated part of the embryo lying between Kupffer's Vesicle and the last 
formed somite. Here, then, metabolism is most active, and the material needed for 
growth ought to be the most abundant. It is now a generally accepted fact that the 
periblast with its giant nuclei play a leading role in the assimilation of the yolk. 
Passing upward from the deeper layers of the yolk, we find that the yolk spheres 
become successively smaller, while in the periblast syncytium they are reduced to 
minute granules. Finally, there seems to occur a thin fluid layer between the peri- 
blast and the cells of the embryo proper. I have already noted the occurrence of a 
large vesicle in the yolk beneath the growing region of the embryo in certain fishes. 
Around this vesicle the periblast and its contained nuclei are especially abundant, 
and within its interior there is to be seen in sections a fine coagulum, reticulate in 
appearance, which seems to be of an albuminous nature. It has for some time been 
my view (SUMNER, '99, a), that this vesicle contains a more fluid yolk, partially 
assimilated through the activity of the periblast, and intended for the nourishment 
of the growing embryo. I have, also expressed the view ('99, b) that Kupffer's 
Vesicle itself represents an embryonic digestive organ (more properly an organ of 
absorption). Its relation to the yolk vesicle has already been dwelt upon. Within 
it, moreover, occurs a coagulum which is similar to that found in the other. 
SOBOTTA ('98) has commented upon the fact that Kupffer's Vesicle contains a fluid 
having a refractive index higher than that of water. I find in the case of Scorpsena, 
that this is noticeably true at an advanced stage in the development of the vesicle, 



SUMNER: KUPFFER'S VESICLE 77 

when the outlines of the chorda, seen through the contents of the former, are 
considerably distorted, this fluid mass serving as a bi-convex lens. 

Microchemical tests as to the contents of the vesicle gave only negative results. 
I subjected the eggs of. Mtirasnaf and Scorpsena to the reagents employed by LE 
DANTEC ('90) and Miss GREENWOOD ('94) for the detection of acid in the vacuoles 
of protozoa. None of these were found applicable to fish eggs, as they either killed 
the embryo or failed to stain it; but I also used Bismarck Brown. This stain, when 
neutral, shows a very characteristic reaction in the presence of acids. But no appre- 
ciable reaction was exhibited by the contents of Kupffer's Vesicle. 



IX. RECAPITULATION. 

1. The hypoblast arises in connection with an invagination of the superficial 
layer ("Deckschicht") occurring on the posterior border of the blastoderm. This 
invagination may be an open one (" Munena " possibly some others) or it may be a 
solid ingrowth of cells (Amiurus, Notwrus, Salvelinus, Fandulus, Ctenolabrus. A 
similar condition was found in Amia. 

2. This invagination is the " prostoma " of KUPFFER, whose descriptions and 
theoretical conclusions upon this subject are in the main correct. 

3. Kupffer's Vesicle arises from the expanded inner end of this invagination, 
when it is an open one ; it is secondarily formed in the invaginated mass of cells, 
when solid. 

4. Kupffer's Vesicle, as is usually stated, represents the post-anal-gut, the neur- 
enteric canal being represented by the open duct leading from the vesicle to the 
exterior in " Murxna " by the solid ingrowth in the other forms named. 

5. A process occurs in the teleosts exactly similar to that folding off of the tail 
end of the embryo which results in the formation of the neurenteric canal of the 
elasmobranchs. The main difference between the two cases is that the teleost embryo 
continues to grow backward at an equal pace with the blastoderm margin, while in 
the elasmobranch, the embryo, owing to its relatively slower growth, is left behind, 
thus losing its continuity with the border of the blastoderm. 

6. I have adduced evidence of a purely morphological character for a view of 
concrescence which is supported by the most recent experimental work. This is, 
briefly, that true concrescence (apposition) occurs at an early period in embryo for- 
mation. It ceases with the appearance of the caudal knob, which arises as a result 
of the above-mentioned folding-off of the neurenteric canal. No true concrescence 



78 MEMOIRS OF THE NEW YORK ACADEMY OF SCIENCES 

can occur after this event, though it is probable from the experiments of MORGAN and 
KOPSCH, [See also Supplement] that a modification of the process, which I have 
termed " CONFLUENCE," continues till the closure of the blastopore. 

7. A second vesicle, lying in the yolk below the embryo, is present in Noturus 
and some other forms. 

8. Kupffer's Vesicle has an important function in embryonic life. Its position 
and some other facts suggest that it plays the part of a transitory digestive (absorbent) 
organ. 

DEPARTMENT OF NATURAL HISTOHY, COLLEGE OF THE CITY OF NEW YORK, April 14, 1900. 



X. NOTE ON METHODS. 

As the result of considerable experimenting upon the fixing of teleost eggs, I 
have settled upon two methods which I now use almost exclusively. One of these 
is treatment with ZENKER'S Fluid. This reagent gives very good results with the 
eggs of Noturus and Amiurus, but I have found it to be far less satisfactory for fixing 
the eggs of the trout or those of pelagic fishes. The second method, which I have 
found applicable to all the eggs I have studied, consists in a brief fixation in subli- 
mate acetic (10^ acetic) followed by preservation in formalin. The eggs are allowed 
to remain in the fixing fluid till the blastoderm or embryo becomes whitened, one 
minute being usually sufficient. After hasty rinsing in water, they are transferred 
to 10^> formalin. This method, in addition to securing good histological fixation, 
has the advantage of not hardening the yolk and of preserving the natural appear- 
ance of the egg far better than any other treatment which I know of. This method, 
Doctor STRONG tells me, originated with Doctor C. M. CHILD. 

The eggs of Amia (kindly furnished me by Professor DEAN) were preserved by 
the late Doctor ARNOLD GRAF. Those which I sectioned had been fixed in ZENKER'S 
Fluid or in GRAF'S Chrom-oxalic mixture. (See New York State Hospitals Bulletin, 
Vol. II, 1897.) Both gave satisfactory results. 

The teleost material was stained according to HAIDENHAIN'S "Iron hsematoxylin " 
method. Occasionally I used an anilin counterstain, though this was of no real ad- 
vantage. For the sections of Amia I employed both the " Iron hsematoxylin "and 
DELAFIELD'S hsematoxylin. The latter was far preferable for these eggs. 



SUMNER: KUPFFER'S VESICLE 79 

XI. SUPPLEMENT. 

Since writing the foregoing pages, I have had an opportunity of putting to ex- 
perimental test certain of the views therein maintained. At Naples, during the 
past July, I carried on experiments with a view to determining the manner of for- 
mation of the embryonic body in Exocoetus sp. My results will shortly be pub- 
lished. I will, however, briefly record the confirmation of two of the conclusions 
maintained above. 

First, that the germ-ring passes, in large part at least, into the embryo. Proof : 
Glass needles were inserted into the eggs, piercing the germ-ring far laterad to the 
caudal end of the embryo during an early stage. At a late period, the point of in- 
sertion of the needle was, in certain instances, found to be close beside the caudal 
end of the embryo. In these cases the latter was conspicuously bent as if the pos- 
terior part had been drawn towards the needle. One can only conclude that the 
segment of the germ-ring which lay between the needle and the embryo had passed 
into the latter, but the germ-ring being held fast on one side, the embryonic body 
itself was drawn in that direction. 

Second, that, at least after a certain period, there occurs a process of confluence, 
rather than one of concrescence. Proof: In cases where a needle was inserted at 
the mid-caudal point of the early embryo, the embryo none the less continued to 
grow, but necessarily in a forward instead of a backward direction, while the blasto- 
pore continued to close in a seemingly normal manner. Indeed there was nothing 
to show that the usual concentric growth of the blastoderm had been disturbed. 
The only possible inference is that the germ-ring material entered at a point anterior 
to the needle causing the embryo to elongate in the only direction in which it was 

free to move. 

FRANCIS B. SUMNER. 

C. C. N. Y. Sept. 21, 1900. 



80 MEMOIRS OF THE NEW YORK ACADEMY OF SCIENCES 



BIBLIOGRAPHY. 

This table includes only those papers referred to in the present article and lays 
no claim to completeness. The papers of Henneguy ('88), Mclntosh and Prince 
('90) and Berent ('96) contain lists of papers bearing on early teleost development, 
and Morgan ('95) and Kopsch ('99) give the literature of concrescence. Kopsch 
('00) gives a very complete bibliography of KupfFer's Vesicle. (This last appeared 
after the present memoir had gone to press.) 

Agassiz, A., and Whitman, C. 0. 

'84. On the Development of some Pelagic Fish Eggs. Preliminary notice. 
Proc. Amer. Acad. Arts and Sciences, Vol. XX, pp. 2375, pi. I. 

Assheton, R. 

'96. An Experimental Examination into the Growth of the Blastoderm of the Chick. 
Proc. Roy. Soc. London, Vol. LX, pp. 349-356. 

Balfour, F. M. 

'78. A Monograph on the Development of Elasmobranch Fishes. London, 1878. 

Balfour, F. M. 

'81. Comparative Embryology. London, 1881. 

Berent, W. 

'96. Zur Kenntniss der Parablastes und der Keimbliitterdifferenzierung im Ei der Knoch- 

enfische. 
Jenaische Zeitscltrift, Bd. 30, pp. 291-349, Taf. XVI-XVIII. 

Clapp, Cornelia M. 

'91. Some Points in the Development of the Toad Fish (Hatrachus tau). 
Journ. Morph., Vol. V, pp. 494-501. 

Corning, H. K. 

'96. Merocyten und Umwachsungsrand bei Teleostiern. 

Festschrift /. Carl Gegenbaur, Bd. 2, pp. 103-132. 
Dean, B. 

'95. The Early Development of Gar, Pike and Sturgeon. 

Journ. Morph., Vol. XI, pp. 1-63, pi. I-IV. 
Dean.'B. 

'95. Fishes, Living and Fossil. New York, Macmillan Co. 

Dean, B. 

'96. The Early Development of Amia. 

Q. J. Micr. Sci., Vol. 38, pp. 413-445, pi. XXX-XXXII. 

Duval, M. 

'84. De la Formation du Blastoderme dans 1'Oeuf d'Oiseau. 

Ann. des Sci. not., Ser. 6, Tome XVIII, pp. 1-208, pi. I-V. 



SUMNER : KUPFFER'S VESICLE 81 

Eigenmann, C. H. 

'94. On the Viviparous Fishes of the Pacific Coast of North America. 

Bull, of U. S. Fish Com. for 1892, pp. 381-478, pi. XCII-CXVIII. 

Eycleshymer, A. 0. 

'95. The early Development of Amblystoma, with Observations on some other Vertebrates. 
Journ. Morph., Vol. X, pp. 343-419, pi. XVIII-XXII. 

Gbtte, A. 

'73. Beitriige zur Entwickelungschichte der Wirbelthiere. I, Der Keim des Forelleneies. 
Arch. f. mikr. Anat., Bd. IX, pp. 679-709, Taf. XXVII. 

Greenwood, (Miss) M. and Saunders, E. R. 

'94. On the Role of Acid in Protozoan Digestion. 

Journ. ofPhys., Vol. XVI, pp. 441-467, 1 pi. 

Gregory, E. 

'99. Die Kupffer'sche Blase bei der Forelle (Trutta fario). 

Festschrift zum siebenzigsten Geburtstag von Karl von Kupffer, pp. 711715, Taf. 

LX, LX1. 
Henneguy, F. 

'88. Becherches sur le Developpement des Poissons ossenx. Embryogenie de la Truite. 
Journ. de I' Anat. et de Physiol., 1888, pp. 413-502 and 525-617, pi. XVIII- 
XXI. 

His, W. 

'74. Unsere Korperform. 

Leipzig, 1874, pp. 186-191. 

His, W. 

'91. Zur Frage der Langsverwachsung von Wirbelthierembryonen. 
Verh. d. anat. Ges., 1891, pp. 70-83. 

Jablonowski. 

'98. Ueber einige Vorgiinge in der Entwickelung des Salmonidenembryos, nebst Bemer- 
kungen iiber ihre Bedeutung fiir die Beurteilung der Bildung des Wirbeltierkorpers. 
Anat. Anz., Jahrg. 14, pp. 532551. 

Jhering, H. von. 

'88. Ueber Brutpflege und Entwickelung des Bagre. 
Biolog. Centr., Bd. VIII, pp. 268-271. 

Kingsley, J. S., and Conn, H. W. 

'83. Some Observations on the Embryology of the Teleosts. 

Memoirs Bost. Soc. Nat. Hist., Vol. Ill, pp. 183-212, pi. XIV-XVJ. 

Kopsch, F. 

'96. Experimented Untersuchungen iiber den Keimhautrand der Salmoniden. 
Verh. d. anat. Ges., 1896, pp. 113-127. 

Kopsch, F. 

'99. Die Organisation der Hemididymi und Anadidymi der Knochenfische. 
Internat. Monatschr. f. Anat. u. Phys., 1899. 



82 MEMOIRS OF THE NEW YORK ACADEMY OF SCIENCES 

Kopsch, F. 

'00. Homologie und Phylogenetische Bedeutung der Kupffer'schen Blase. 
Anat. Anz., Bd. XVII, pp. 497-509. 

Kowalewski, M. von. 

'85. Ueber Furchung und Keimblatteranlage der Toleostier. 

Sitzungsber. d. phys.-med. Soc. zu Erlangen, Heft 18, pp. 1-6. 

Kowalewski, M. von. 

'86a. Ueber die ersten Entwickhingsprocesse der Knochenfische. 
Zeitschr.f. wiss. ZooL, Bd. 43, pp. 434-480, pi. XVII. 

Kowalewski, M. von. 

'86b. Die Gastrulation und die sogen. Allantois bei den Teleostiern. 

Sitzungsber. d. phys.-med. Soc. u. Erlanrjen, Heft 18, pp. 3136, Taf. I. 

Kupfler, C. von. 

'66. Untersuchungen iiber die Entwicklungen des Harn- und Geschlechtssystems. (Second 

part.) 
Arch.f. mikr. Anat., bd. II., pp. 473-489, Taf. XXIV. 

Kupffer, C. von. 

'79. Die Enstehung der Allantois und die Gastrula der Wirbelthiere. 
ZooL Anz., 1879, pp. 520-522, 593-597, 612-617. 

Kupffer, C. von. 

'84. Die Gastrulation an den meroblastischen Eiern der Wirbelthiere und die Bedeutung 

des Primitifstreifs, III. 
Arch.f. Anat. u. Phys. (Anat. Abth.\ 1884, pp. 1-40, Taf. I, II. 

Le Dantec, F. 

'90. Recherches sur la Digestion intracelhilaires chez les Protozoaires. 
Ann. de Vlnstitut Pasteur, 1890, p. 776. 

Lereboullet, M. 

'63. Recherches sur les Monstruosites du Brochet. 

Ann. des Sci. nat., 4 Serie, Tome XX, pp. 177-271, pi. 2, 3. 

Locy, W. A. 

'95. Contribution to the Structure and Development of the Vertebrate Head. 
Journ. Morph., Vol. XI, pp. 497-594, pi. XXVI-XXX. 

M'Intosh, W. C., and Prince, E. E. 

'90. On the Development and Life History of the Teleostean Food- and other Fishes- 
Trans. Roy. Soc. Edinb., Vol. XXXV, pp. 665-946, pi. I-XXVIII. 

Morgan, T. H. 

'93. Experimental Studies on the Teleost Eggs. 
Anat. Am., Jahrg. 8, pp. 803-814. 

Morgan, T. H. 

'95. The Formation of the Fish Embryo. 

Journ. Morph., Vol. X, pp. 419-472, pi. XXIII-XXV. 



SUMNER: KUPFFEK'S VESICLE 83 

Oellacher, J. 

'72. Beitrage zur Entwicklungsgeschichte der Knochcnfisclie nach Beobachtungen am 

Bachforelleneie. 
Zeitschr.f. wiss. ZooL, Bd. 22, pp. 373-421, Taf. XXXII, XXXIII. 

Raffaelle, F. 

'88. Le uova galleggianti e le larve del Teleostei nel golfo di Napoli. 

Mitth. aus der Zool. Station zu JVeapel, Bd. 8, pp. 184, pi. 1-5. 

Reinhard, W. 

'98. Die Bedeutung des Periblastes und der Kupffer'schen Blase in der Entwiokelung der 

Knoehenfisclie. 
Arch./, mikr. Anat., 1898, pp. 793-819, Taf. XXXV-XXXVI. 

Ryder, J. A. 

'86. On the Development of Osseous Fishes. 

Rep. of U. S. Fish Com. for 1885, pp. 116, pi. XXX. 
Schwarz, D. 

'89. Untersuchungen des Schwanzes bei den Embryonen der Wirbelthiere. 
Zeitschr. f. wiss. ZooL, Bd. 48, pp. 191-223, Taf. XII-XIV. 

Sobotta, J. 

'96. Die Gastrulation von Amia calva. 

Verh. d. anat. Ges., 1896, pp. 108-111. 

Sobotta, J. 

'98. Die morphologische Bedeutung der Kupffer'schen Blase. 

Verh. d. phys. med. Ges. zu Wurzburg, Bd. XXXII, pp. 16, 1 pi. 

Sumner, F. B. 

(See below.) 

Virchow, H. 

'95. Ueber das Keimhautrand der Salmoniden. 
Verh. d. anat. Ges., 1895, pp. 201-219. 

Wilson, H. V. 

'91. The Embryology of the Sea Bass (Serranus atrarius). 

Bull. U. S. Fish Com. for 1889, pp. 209-277, pi. LXXXVIII-CVII. 



The following is a list of brief abstracts of papers read by the author of the pres- 
ent memoir and referred to in the text : 

1. 1899 a. On the Early Development of the Catfish (Noturus). Read before Amer. Mbrph. 

>Soc. Dec. 28, 1898. 

Abstracted in Science, Mar. 3, 1899, pp. 313, 314. (No mention is made in the ab- 
stract of the " prostomal thickening " of the blastoderm, although this appearance 
was described in the paper.) 



84 



MEMOIRS OF THE NEW YORK ACADEMY OF SCIENCES 



2. 1899 b. Observations on the Germ-layers of Teleost Fishes. Head before Section of jBi 

JV: Y. Acad. Sci., Mar. 14, 1899 
Abstracted in Science, May 19, 1899, p. 718. 

3. 1900 a. The Teleost Gastrula and its Modifications. Head before Arncr. Morph. Soc. Dec. 

27, 1899. 
Abstracted in Science, Feb. 2, 1900, p. 169. 

4. 1900 b. Kupffer's Vesicle and its relation to Gastrnlation and Concrescence. Read before 

Section of Biology, N. Y. Acad. Sci., Feb. 12, 1900. 
Abstracted in Science, Mar. 16, 1900. 



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