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Environmental  Protection  Technology  Series 


LIBBY,  McNeill  &  LIBBY 

FOOD  TECH.  RES.  LIBRARY 

CHICAGO,  ILLINOIS 


MINIMIZATION  OF  WATER  USE  IN  LEAFY 

VEGETABLE  WASHERS 


Industrial  Environmental  Research  Laboratory 

Office  of  Research  and  Development 

U.S.  Environmental  Protection  Agency 

Cincinnati,  Ohio  45268 


RESEARCH  REPORTING  SERIES 

Research  reports  of  the  Office  of  Research  and  Development,  U.S.  Environmental 
Protection  Agency,  have  been  grouped  into  nine  series.  These  nine  broad  cate- 
gories were  established  to  facilitate  further  development  and  application  of  en- 
vironmental technology.  Elimination  of  traditional  grouping  was  consciously 
planned  to  foster  technology  transfer  and  a  maximum  interface  in  related  fields. 
The  nine  series  are: 

1.  Environmental  Health  Effects  Research 

2.  Environmental  Protection  Technology 

3.  Ecological  Research 

4.  Environmental  Monitoring 

5.  Socioeconomic  Environmental  Studies 

6.  Scientific  and  Technical  Assessment  Reports  (STAR) 

7.  Interagency  Energy-Environment  Research  and  Development 

8.  "Special"  Reports 

9.  Miscellaneous  Reports 

This  report  has  been  assigned  to  the  ENVIRONMENTAL  PROTECTION  TECH- 
NOLOGY series.  This  series  describes  research  performed  to  develop  and  dem- 
onstrate instrumentation,  equipment,  and  methodology  to  repair  or  prevent  en- 
vironmental degradation  from  point  and  non-point  sources  of  pollution.  This  work 
provides  the  new  or  improved  technology  required  for  the  control  and  treatment 
of  pollution  sources  to  meet  environmental  quality  standards. 


This  document  is  available  to  the  public  through  the  National  Technical  Informa- 
tion Service,  Springfield,  Virginia  22161. 


EPA-600/2-77-135 
July  1977 


MINIMIZATION  OF  WATER  USE 
IN  LEAFY  VEGETABLE  WASHERS 


by 

Malcolm  E.  Wright 

Agricultural  Engineering  Department 

and 

Robert  C.  Hoehn 

Civil  Engineering  Department 

Virginia  Polytechnic  Institute  and  State  University 

Blacksburg,  Virginia  24061 


Grant  No.  S-802958 


Project  Officer 

Harold  W.  Thompson 
Industrial  Pollution  Control  Division 
Industrial  Environmental  Research  Laboratory 
Corvallis,  Oregon  97330 


INDUSTRIAL  ENVIRONMENTAL  RESEARCH  LABORATORY 
OFFICE  OF  RESEARCH  AND  DEVELOPMENT 
U.S.  ENVIRONMENTAL  PROTECTION  AGENCY 
CINCINNATI,  OHIO  A5268 


DISCLAIMER 


This  report  has  been  reviewed  by  the  Industrial  Environmental  Research 
Laboratory,  Cincinnati,  U.S.  Environmental  Protection  Agency,  and  approved  for 
publication.   Approval  does  not  signify  that  the  contents  necessarily  reflect 
the  views  and  policies  of  the  U.S.  Environmental  Protection  Agency,  nor  does 
mention  of  trade  names  or  commercial  products  constitute  endorsement  or 
recommendation  for  use. 


11 


FOREWORD 


When  energy  and  material  resources  are  extracted,  processed,  converted, 
and  used,  the  related  pollutional  impacts  on  our  environment  and  even  our 
health  often  require  that  new  and  increasingly  more  efficient  pollution  control 
methods  be  used.   The  Industrial  Environmental  Research  Laboratory  -  Cincinnati 
(lERL-Ci)  assists  in  developing  and  demonstrating  new  and  improved  methodo- 
logies that  will  meet  these  needs  both  efficiently  and  economically. 

This  report  covers  the  construction  and  evaluation  of  an  improved 
leafy  greens  vegetable  washing  system.   This  system  consisted  of  two  series 
drum  immersion  washers,  each  with  associated  settling  tanks  and  moving  belt 
screens.   Wash  water  was  used  in  a  counter-current  flow  regime.   Results 
obtained  when  comparing  the  prototype  process  to  current  commercial  washing 
systems  were  encouraging.   Significant  reductions  in  wash  water  requirements 
and  wastewater  generation  were  reported;  as  was  an  increase  in  cleaning 
efficiency. 

It  appears  that  this  process  modification  will  become  a  building  block 
in  the  development  of  economically  achievable  waste  management  systems  for 
the  leafy  greens  processing  industry.   As  a  result  this  report  should  be  of 
interest  to  processors  of  leafy  greens,  designers  of  processing  facilities, 
equipment  manufacturers  and  environmental  regulatory  agencies. 

Further  information  on  this  project  can  be  obtained  by  contacting  the 
Food  and  Wood  Products  Branch  of  lERL-Ci. 


David  G.  Stephan 
Director 
Industrial  Environmental  Research  Laboratory 
Cincinnati 


111 


ABSTRACT 

This  project  was  undertaken  to  construct  and  test  an  improved  leafy 
greens  washing  system  employing  water  recirculation,  to  characterize  the 
quality  of  the  wash  water  and  waste  stream,  and  to  make  comparisons  to  con- 
ventional washers.   The  prototype  system  produced  a  cleaner  product  while 
reducing  water  requirements  and  consolidating  waste  loads. 

The  prototype  system  consisted  of  two  drum  immersion  washers  in  series, 
each  with  associated  settling  tanks,  filters,  and  water  recirculation  systems. 
Construction  was  similar  to  conventional  washers  but  with  modifications  to 
improve  removal  of  floating  trash  and  increase  hydraulic  agitation  of 
product.   Fresh  water  input  was  limited  to  that  required  to  replace  water 
carried  off  by  the  product  plus  a  small,  overflow,  effluent  stream  from  the 
system. 

The  prototype  was  tested  in  a  commercial  processing  plant  during  the 
fall  and  spring  harvesting  seasons,  1975-76.   Sixty-seven  metric  tons  of 
collards,  spinach,  and  turnip  greens  were  processed  through  the  prototype 
in  52  hours  of  actual  operating  time.   Conventional  washers  were  monitored 
for  27  hours  (38  tons)  for  comparison.   Insect  and  bacteria  counts,  COD, 
TSS ,  VSS ,  and  several  other  water  and  product  parameters  were  measured  at 
predetermined  times  and  locations.   Data  were  obtained  to  predict  expected 
waste  loads  from  the  products  processed. 

Economic  considerations  indicate  that  the  annual  fixed  costs  of  owning 
the  prototype  system  would  be  approximately  $600  per  year  more  than  the  costs 
of  owning  a  conventional  system,  of  comparable  capacity.   Operating  costs, 
however,  were  $100/day  less  for  the  prototype  than  for  the  conventional 
system  in  an  example  problem  using  conditions  similar  to  those  at  the  test 
site.   These  results  would,  of  course,  vary  considerably  depending  on  local 
utility  rates  and  other  operating  costs. 

This  report  was  submitted  in  fulfillment  of  Grant  No.  S802958  by  the 
Virginia  Polytechnic  Institute  and  State  University  under  the  partial 
sponsorship  of  the  Environmental  Protection  Agency.   This  report  covers  the 
period  from  May  1,  1974  to  January  31,  1977,  and  work  was  completed  as  of 
January  31,  1977. 


IV 


CONTENTS 


Foreword iil 

Abstract iv 

Figures vi 

Tables ix 

Abbreviations   xi 

Acknowledgment  xii 

1.  Introduction  1 

2.  Conclusions  3 

3.  Recommendations  5 

4.  Prototype  Washer  System  7 

Washer  design  7 

Water  flow  Instrumentation 15 

Installation  and  modifications   19 

5.  Procedures 22 

Overview 22 

Specific  procedures  27 

6.  Results  and  Discussion 34 

Operating  parameters   34 

Product  quality  parameters   48 

Water  quality  parameters  52 

Summary  of  waste  production  from  washers   62 

Economic  comparisons   66 

References 70 

Appendices 72 

A.  Operating  parameters  data 72 

B.  Product  quality  data 80 

C.  Water  quality  data 84 


FIGURES 
Number  Page 

1  Diagram  of  washing  system  showing  water  and  product  flow 

patterns,  sampling  sites,  and  water  flow  meter  locations  ...   8 

2  Overhead  view  of  washing  system  adjacent  to  Exmore  plant. 

Rotary  sand  tumbler  and  conveyor  into  plant  are  at  right 

and  foreground 9 

3  Diagram  of  prototype  washer   10 

4  Paddle  wheel  showing  expanded  metal  covering  and  spoked  end 

construction.   Elevated  nozzle  banks  are  shown  in  fore- 
ground   12 

5  Exit  conveyor  of  washer  number  2 13 

6  View  of  washer  side  drains  in  operation 14 

7  Moving  belt  screen  in  operation  16 

8  Moving  belt  screen  and  trash  collector.   Compressed  air  hose 

for  removing  trash  from  belt  is  shown  in  foreground 17 

9  Prototype  settling  tank   18 

10  HS  flume  meter  number  4  with  water  level  recorder   20 

11  HS  flume  meter  number  5  with  water  level  recorder 21 

12  Schematic  showing  water  and  product  sampling  sites  for 

comparative  study  of  new  vs.  conventional  leafy  greens 

washing  systems  at  Exmore  Foods,  Exmore,  Va 24 

13  Water  flow  rates  vs.  operating  time,  trial  1,  Fall,  1975, 

when  processing  collards  with  prototype  system.   Refer  to 

Figure  1  for  meter  locations 37 

14  Water  flow  rates  vs.  operating  time,  trial  1,  Spring, 

19  76,  when  processing  spinach  with  prototype  system. 

Refer  to  Figure  1  for  meter  locations 37 


VI 


Number  Page 

15  Water  overflow  rates  from  conventional  washers  vs. 

operating  time,  trial  1,  Spring,  1976,  when  processing 
spinach  on  the  east  line 37 

16  Product  flow  rate  vs.  operating  time,  trial  2,  Fall,  1975, 

when  processing  collards  with  the  prototype  system  ....   41 

17  Accumulated  product  input  vs.  operating  time,  trial  2, 

Fall,  1975,  when  processing  collards  with  the  prototype 

system 41 

18  Summation  percentages  vs.  particle  size  for  grit 

accumulated  in  the  prototype  system  sub-unit  1;  trial 

5,  Fall,  1975,  when  processing  spinach  47 

19  Summation  percentages  vs.  particle  size  for  grit 

accumulated  in  the  prototype  system  sub-unit  2;  trial 

5,  Fall,  1975,  when  processing  spinach  47 

20  Summation  percentages  vs.  particle  size  for  grit 

samples  taken  from  conventional  Washer  1,  East  Line, 

trial  2,  Spring,  1976  when  processing  spinach A7 

21  Grit  (inorganic  solids)  on  spinach  vs.  accumulated  product 

at  three  sites  in  prototype  system,  trial  1,  Spring; 

unwashed  product  (Site  1) ,  product  exiting  first  washer 

(Site  3) ,  product  exiting  second  washer  (Site  4)  51 

22  Grit  (inorganic  solids)  on  spinach  vs.  accumulated  product 

at  three  sites  in  conventional  system,  trial  1,  Spring; 
unwashed  product  (Site  7) ,  product  exiting  first  washer 
(Site  8) ,  product  exiting  second  washer  (Site  10)   ....   51 

23  Grit  (inorganic  solids)  on  turnip  greens  vs.  accumulated 

product  at  three  sites  in  prototype  system,  trial  6, 
Spring;  unwashed  product  (Site  1) ,  product  exiting  first 
washer  (Site  3)  ,  product  exiting  second  washer  (Site  4)   .   51 

24  Grit  (inorganic  solids)  on  turnip  greens  vs.  accumulated 

product  at  three  sites  in  conventional  system,  trial  6, 
Spring;  unwashed  product  (Site  12) ,  product  exiting  first 
washer  (Site  14) ,  product  exiting  second  washer  (Site  15) .   51 

25  Total  bacterial  plate  counts  per  gram  of  spinach  at  three 

sampling  points,  trial  1,  Spring;  prototype  system. 
Before  washing  (Site  1) ,  exiting  the  first  washer 
(Site  3)  ,  exiting  the  second  washer  (Site  4) 53 


Vll 


Number  Page 

26  Total  bacterial  plate  counts  per  gram  of  turnip  greens  at 

three  sampling  points,  trial  6,  Spring;  prototype  system. 
Before  washing  (Site  1)  ,  exiting  the  first  washer  (Site  3) , 
exiting  the  second  washer  (Site  4)  53 

27  Total  bacterial  plate  counts  per  gram  of  spinach  at  three 

sampling  points,  trial  1,  Spring;  conventional  system. 
Before  washing  (Site  7) ,  exiting  the  first  washer  (Site  9) , 
exiting  the  second  washer  (Site  10)   53 

28  Total  bacterial  plate  counts  per  gram  of  turnip  greens  of 

three  sampling  points,  trial  6,  Spring;  conventional  system. 
Before  washing  (Site  12) ,  exiting  the  first  washer  (Site  14) , 
exiting  the  second  washer  (Site  15)   53 

29  Bacterial  populations  and  chlorine  residual  in  wash  water  at 

Site  1  of  prototype,  trial  2,  Fall,  when  processing 

collards 56 

30  Bacterial  populations  and  chlorine  residual  in  wash  water 

at  Site  4  of  prototype,  trial  2,  Fall,  when  processing 

collards 56 

31  Total  suspended  solids  vs.  accumulated  product  input  at  all 

six  sampling  sites,  trial  4,  Fall,  when  processing  collards 
with  prototype  system  58 

32  Chemical  oxygen  demand  vs.  accumulated  product  at  all  six 

sampling  sites,  trial  4,  Spring,  when  processing  turnip 

greens  with  prototype  system   58 

33  Total  suspended  solids  vs.  accumulated  product  at  all  four 

sampling  sites,  trial  1,  Spring,  spinach  processed  with 
conventional  washer   58 

34  Chemical  oxygen  demand  vs.  accumulated  product  at  all 

four  sites,  trial  6,  Spring,  turnip  greens  processed 

with  conventional  system  58 

35  Five-day  biochemical  oxygen  demand  vs.  color,  trial  2, 

Fall,  when  processing  collards  with  prototype  system  ....  61 

36  Five-day  biochemical  oxygen  demand  vs.  color,  trial  3, 

Fall,  when  processing  collards  with  prototype  system  ....  61 

37  Five-day  biochemical  oxygen  demand  vs.  color,  trial  4, 

Fall,  when  processing  collards  with  prototype  system  ....  61 


Vlll 


TABLES 
Number  Page 

1  Summary  of  Information  for  Trials  of  Prototype  Washer  System 

During  the  Fall  Season  of  1975 25 

2  Summary  of  Information  for  Trials  of  Prototype  and  Conventional 

Washing  System  During  the  Spring  Season  of  1976   26 

3  Average  Water  Use  Date  for  Prototype  Leafy  Vegetable  Washing 

System  During  Fall  Trials,  1975 35 

4  Average  Water  Use  Data  for  Prototype  Leafy  Vegetable  Washing 

System  During  Spring  Trials,  1976   36 

5  Average  Water  Use  Data  for  Conventional  Leafy  Vegetable 

Washers  During  Spring  Trials,  1976   39 

6  Product  Data  for  Prototype  Leafy  Vegetable  Washing  System 

During  Fall  Trials,  1975 42 

7  Product  Data  for  Prototype  Leafy  Vegetable  Washing  System 

During  Spring  Trials,  1976  43 

8  Product  Data  for  Conventional  Leafy  Vegetable  Washers  During 

Spring  Trials,  1976 44 

9  Dry  Weight  of  Grit  from  Various  Units  of  the  Prototype  Leafy 

Vegetable  Washer  at  End  of  Each  Trial 46 

10  Accumulation  of  Floating  Trash  from  Settling  Tank  Moving  Belt 

Screens  for  Prototype  System  During  Fall  and  Spring  Trials  •  •   49 

11  Comparisons  of  Bacterial  Population  Densities  (Total  Plate  Counts) 

for  Product  Leaving  to  Product  Entering  a  Two-Washer  System 
and  for  Water  Leaving  the  Second  Washer  to  Water  Entering  the 
First  During  Greens  -  Washing  Trials  54 

12  Magnitude  of  Average  Changes  in  Total  Plate  Counts  From  Beginning 

to  End  of  Trials  at  all  Sampling  Sites  Recorded 57 

13  Concentration  of  Phosdrin  in  Water  of  First  Washer  of  Prototype 

System  ,  Spring  Trials  60 


XX 


Number  Page 

14  Waste  Loads  Discharged  with  Water  from  Prototype  System  During 

Fall  Trials,  1975 63 

15  Waste  Loads  Discharged  with  Water  from  Prototype  System  During 

Spring  Trials,  1976 64 

16  Waste  Loads  Discharged  with  Water  from  Conventional  Washers 

During  Spring  Trials,  1976   65 

17  Waste  Stream  Characteristics  From  Prototype  and  Conventional 

Systems 57 


ABBREVIATIONS 


BOD  —  biochemical  oxygen  demand 

BODc  —  five-day  biochemical  oxygen  demand 

BOD„-.  —  twenty-day  biochemical  oxygen  demand 

COD  —  chemical  oxygen  demand 

TS  —  total  solids 

TSS  —  total  suspended  solids 

VSS  —  volatile  suspended  solids 

SS  —  suspended  solids 

0„  —  oxygen 

metric  ton  —  1000  kilograms 

PVC  —  polyvinylchloride 


XI 


ACKNOWLEDGMENTS 

The  most  generous  cooperation  of  the  personnel  of  Exmore  Foods,  Exmore, 
Virginia  is  gratefully  acknowledged.   The  open-handed  willingness  to  allow 
the  use  of  plant  space,  utilities,  and  personnel  time  in  the  conduct  of  this 
project  was  exemplary,  indicating  a  far-sightedness  that  transcends  immediate 
gain.   The  results  of  this  work  will  carry  much  additional  weight  by  virtue 
of  the  tests  being  performed  in  a  practical,  working  environment. 

Principals  to  be  cited  include  Mr.  Caspar  Battaglia,  President  of  Exmore 
Foods,  for  his  suggestion  that  his  plant  be  used  for  the  test  site  and  his 
continued  interest  throughout  the  project;  Mr.  Charles  Floyd,  Plant  Manager, 
for  his  cooperation  in  day-to-day  arrangements;  Mr.  Stoakely  Pearson,  Plant 
Engineer,  for  the  skill,  care,  and  energy  exerted  in  getting  the  equipment 
installed  and  making  certain  necessary  modifications;  and  Mrs.  Lucille  Floyd, 
Mr.  Woodrow  Brawley,  and  Mr.  James  Morrison  for  allowing  the  unrestrained 
use  of  their  laboratory.   Many  others  should  be  cited,  particularly  the 
foremen  and  workers  on  the  processing  lines.   Their  patience,  humor,  and 
apparent  pride  in  being  associated  with  the  project  were  a  source  of  inspira- 
tion, especially  during  trying  moments. 

Special  recognition  is  extended  to  the  graduate  students  associated 
with  the  project,  particularly  Bill  Robinson,  Paige  Geering,  and  Jim  Coleman. 
Several  others  also  made  significant  contributions.   The  kind  and  amount 
of  work  that  they  were  subjected  to  and  the  inconveniences  of  the  travel 
imposed  were  uncoimnon  compared  to  usual  graduate  studies.   Their  response 
and  enthusiasm  were  also  uncommon  —  well  above  the  ordinary  requirements 
implied  by  the  receipt  of  stipends  and  degrees. 


Xll 


SECTION  1 

INTRODUCTION 

A  1971  estimate  by  the  National  Canners  Associations  indicated  that  the 
1838  fruit  and  vegetable  canning  and  freezing  plants  in  the  U.  S.  used  99 
billion  gallons  of  water  and  discharged  96  billion  gallons  of  wastewater  (14). 
Approximately  626  million  pounds  of  leafy  greens  and  broccoli  were  processed 
in  80  plants  during  that  same  year  (6)  (21)  requiring  an  estimated  2.5  billion 
gallons  of  process  water.   Even  though  greens  processing  represents  only  a 
small  percentage  of  the  fruit  and  vegetable  industry  output,  research  in 
areas  related  to  it  can  have  general  applicability  in  many  instances. 

Two  major  concerns  of  leafy  greens  processors  are  water  use  management 
and  initial  cleaning  of  freshly  harvested  product.   Concerns  in  water 
management,  particularly  those  related  to  effluents,  have  assumed  added 
importance  in  recent  years  relative  to  the  new  emphases  on  environmental 
protection.   Major  problems  have  arisen  in  handling  effluents  from  the  lack 
of  knowledge  of  waste  stream  characteristics.   Design  information  on  waste- 
water parameters  for  treating  combined  flows  from  fruit  and  vegetable 
processing  is  sketchy  at  best.   Flow  and  concentrations  of  waste  stream 
constituents  from  unit  operations  within  plants  are  even  less  available. 

A  limited  amount  of  information  is  available  on  combined  waste  stream 
loadings  from  leafy  vegetable  processing  in  reports  by  Mercer  (12) , 
Ramseier  (16),  Frey  (8)  (9),  the  NCA  (14)  and  SCS  Engineers  (18).   Carter  (4), 
Bough  (2),  Frey  and  SCS  Engineers  have  reported  on  certain  unit  operations. 
The  data  available,  however,  are  still  inadequate  for  proper  design  of  in- 
plant  or  out-of-plant  waste  stream  management.   The  parameters  reported  vary 
from  study  to  study  and  might  include  any  of  the  following:   BOD,  COD,  TS, 
TSS,  VSS,  dissolved  0„,  pH,  alkalinity,  or  bacterial  counts.   Methods  of 
reporting  each  parameter  may  also  vary.   For  instance,  COD  may  variously  be 
given  in  terms  of  miligrams/liter  of  wastewater,  pounds  per  ton  of  product 
processed  or  even  pounds  per  1000  cases  of  canned  product.   Other  important 
information,  such  as  flow  rates  of  product  and  water,  is  often  omitted  or 
crudely  estimated.   Total  water  consumption  has  been  reported  to  range  from  3.2 
to  5.4  gal/lb  of  greens  processed.   Estimates  of  total  water  consumption  re- 
quired for  initial  washing  range  from  68  to  88  percent.   Obviously,  the  major 
volume  of  the  total  wastewater  comes  from  this  source.   While  the  inconsisten- 
cies cited  above  do  not  necessarily  invalidate  reported  results,  they  do  limit 
their  usefulness  and/or  credibility. 

Virtually  no  information  is  available  on  the  relative  effectiveness  of 
different  devices  used  in  the  initial  cleaning  of  various  greens.   Typical 


equipment  used  prior  to  blanching  is  described  by  Carter,  Bough,  Frey  and 
Lopez  (11).   This  usually  includes,  in  order,  a  dry  tumbler  for  removal  of 
loose  soil  and  small  particles,  hand  inspection  and  picking  belts,  and  from 
one  to  four  wet  washers. 

The  present  study  was  initiated  to  address  the  two  major  problems  of 
producing  cleaner  product  in  the  initial  processing  of  leafy  greens  and  to 
characterize  the  waste  streams  from  these  processes.   An  experimental,  two- 
washer,  prototype  system  incorporating  the  principle  of  water  recirculation 
was  constructed  and  tested  during  two  harvesting  seasons  at  a  commercial, 
frozen-vegetable  processing  plant.   Design  of  the  washers  was  based  on 
modifications  of  conventional  washers  developed  by  Frey  to  increase  their 
effectiveness.   High  recirculation  rates  within  the  system  provided  hydraulic 
agitation  to  supplement  the  mechanical  agitation.   Input  water  was  limited 
to  that  required  for  makeup  plus  one  small  waste  stream  from  the  system. 
Water  and  product  quality  and  flow  rates  were  monitored  at  several  points  in 
the  prototype  during  the  fall  of  1975  to  determine  washing  effectiveness  and 
characterize  internal  and  external  water  flows.   A  similar  testing  program, 
conducted  during  the  spring  of  1976,  included  tests  on  conventional  washers 
for  comparison  purposes. 


SECTION  2 

CONCLUSIONS 

The  experimental  prototype  leafy-greens  washing  system  was  more 
effective,  though  not  dramatically  so,  in  removing  grit  and  insects  from 
product  than  the  conventional  washers.   It  also  showed  a  potential  for 
better  control  of  bacteria  counts  on  product  prior  to  blanching.   The  final 
rinse,  with  fresh  chlorinated  water,  appeared  to  be  quite  effective  for  grit 
removal  and  bacteria  control.   There  was  no  apparent  increase  in  grit  or 
insects  on  product  as  washing  proceeded  with  recirculated  water.   A  soap- 
like foam  accumulated  on  the  water  surfaces  of  the  prototype  that  may  have 
had  a  significant  effect  on  product  cleaning. 

Differences  in  water  use  between  the  two  systems  to  obtain  cleaning 
was  dramatic,  the  prototype  using  only  about  1/5  the  amount  of  water  used  by 
the  conventional  washers.   Waste  water  discharge  from  the  prototype  was 
approximately  1/12  that  of  the  conventional  washers.   The  average  amount  of 
water  carried  out  on  product  from  the  prototype  was  2.2  £/kg   (0.26  gal/lb) 
A  fresh  water  input  rate  to  the  system  of  3.5  Ji/kg  (0.42  gal/lb)  is  a 
recommended  minimum. 

The  amount  of  each  type  of  waste  constituent  (TSS,  VSS,  COD)  discharged 
with  the  water  from  the  prototype  system  per  unit  of  product  processed  was 
less  than  that  from  the  conventional  washers  though  the  concentrations  were 
higher.   For  example,  the  average  discharges,  from  each  system  respectively, 
while  processing  turnip  greens  were:   TSS  -  0.26  and  1.54,  VSS  -  0.04  and 
0.26,  COD  -  0.16  and  2.31  kg  per  metric  ton  of  product.   Some  of  this  differ- 
ence, particularly  for  the  non-volatile  solids,  was  due  to  accumulations  in 
the  washers  and  settling  tanks  of  the  prototype  that  could  be  disposed  of 
separately  from  the  waste  stream.   VSS  and  COD  production  in  the  prototype 
was  less  than  in  the  conventional  washers,  probably  due  to  lower  osmotic 
gradients  between  the  recirculated  water  and  the  vegetables.  Average  con- 
centrations in  the  discharge  from  the  prototype  and  conventional  washers, 
respectively,  while  processing  turnip  greens  were:   TSS  -  273  and  85,  VSS  - 
37  and  14,  COD  -  135  and  128  mg/Jl  of  waste  stream. 

Approximately  75  percent  of  the  cleaning  took  place  in  the  first  washer- 
settling  tank  sub-system  of  the  prototype.   Given  steady  inputs  of  product 
and  water  similar  to  the  average  conditions  of  this  study  (1278  kg/hr  and  72 
X-/min),  the  waste  strength  parameters  in  the  washers  and  settling  tanks  will 
stabilize  at  some  maximum  value  after  approximately  5  hours  of  operation. 
This  maximum  value  will  be  affected,  of  course,  by  the  average  "dirtiness" 
of  the  vegetables. 


Waste  production  varies  greatly  between  different  varieties  of  vege- 
tables and  between  different  cuttings  of  the  same  vegetable.   All  para- 
meters —  organic,  inorganic,  insects  and  bacteria  —  are  affected  by  age  at 
harvest,  growing  conditions,  method  of  harvest,  etc.   Of  the  three  varieties 
tested,  spinach  consistently  produced  the  most  TSS  per  unit  of  product  and 
could  be  used  as  a  model  for  design  information  for  this  waste  parameter. 
Other  results,  however,  do  not  indicate  that  any  of  the  three  products 
tested  -  collards,  spinach  or  turnip  greens  —  could  be  used  as  a  general 
model  for  VSS  and  COD  emissions.   Wash  waters  were  generally  neutral  in  all 
trials  indicating  that  pH  would  not  be  a  problem  in  treatment  of  effluents. 

The  mechanical  performance  of  the  prototype  washer  was  very  satisfactory 
though  it  could  be  improved  as  outlined  in  the  recommendations  section. 
Of  particular  note  are  the  product  discharge  conveyor  belts  and  moving  belt 
screens  for  the  recirculated  water.   The  discharge  belts  were  made  of  plastic 
and  appear  to  be  a  very  effective  and  inexpensive  substitute  for  stainless 
steel.   The  screen  belts,  also  of  a  monofilament  plastic,  provided  a 
relatively  simple,  inexpensive  means  of  separating  small  leaf  fragments  and 
even  insects  from  the  wash  water. 

Processors  of  frozen  vegetables  use  large  quantities  of  water  to  cool 
blanched  product  prior  to  packaging.   After  the  cooling  water  is  separated 
from  the  product  it  is  usually  used  in  the  raw  product  washers.   Assuming  that 
alternate  means  of  economically  cooling  product  (by  chilled  air,  for  example) 
can  be  found,  then  freezers,  as  well  as  canners,  of  leafy-vegetables  would 
find  considerable  advantage  in  implementing  low-water-ase  washing  systems. 

Recycling  wash  water  in  the  initial  processing  of  leafy  vegetables  is  a 
viable  means  of  consolidating  wastes,  reducing  the  amount  of  effluent  and 
reducing  the  amount  of  total  water  required.   Increased  hydraulic  agitation 
of  product  by  high  internal  flow  rates  in  the  system  coupled  with  a  final 
rinse  of  controlled  chlorine  content  can  improve  vegetable  cleaning  compared 
to  conventional  washers.   These  findings  are  significant  in  terms  of 
environmental  protection,  resource  conservation,  and  food  quality.   They 
indicate  that  the  final  efforts  needed  to  encourage  implementation  by  the 
food  industry  should  be  taken. 

The  intial  cost  of  the  prototype  system  developed  in  this  study  was 
estimated  at  $16,000  compared  to  $12,000  for  a  conventional  system  of 
equivalent  capacity.   Annual  fixed  costs  of  ownership  were  $2208  and  $1656, 
respectively, for  a  difference  of  $552  per  year.   Assuming  a  product  mix  of 
3/4  spinach  and  1/4  turnip  greens,  operating  conditions  similar  to  those  in 
this  study,  and  using  local  labor  and  utility  costs,  the  daily  operating 
cost  for  the  prototype  was  $158  and  for  the  conventional  washers  $251   an 
advantage  of  $93  per  day  for  the  prototype.   The  difference  in  annual  fixed 
costs,  in  this  example  then,  were  recovered  in  approximately  six  days  of 
operation.   If  this  is  considered  representative,  then  the  economics  of 
owning  and  operating  the  two  systems  strongly  favor  the  low-water-use 
prototype  washing  system. 


SECTION  3 

RECOMMENDATIONS 

The  prototype,  leafy- vegetable  washing  system  is  effective  in  cleaning 
leafy  vegetables  while  using  a  minimum  amount  of  water.   No  changes  in  its 
functional  design  are  considered  to  be  necessary  at  this  time.   This  does 
not  imply,  however,  that  the  effectiveness  of  the  system  could  not  be 
improved  by  study  of  additional  components  or  techniques  in  operation.   The 
relative  effectiveness  of  the  new  system  compared  to  similar  conventional 
washers  does  seem  to  warrant  its  adoption  by  the  food  processing  industry 
as  soon  as  possible. 

The  present  prototype  has  some  limitations,  unrelated  to  function, 
that  need  improvement  prior  to  considering  it  for  commercial  use  over  an 
extended  period.   As  now  constructed,  it  requires  too  much  space  and  is 
too  complicated.   These  problems,  however,  can  easily  be  overcome  by  a 
redesign  that  will  not  affect  system  performance  and  might  possibly  improve 
it.   For  example,  overflow  water  from  each  washer  is  now  collected  in  a 
sump  and  pumped  over  a  moving-belt  screen  prior  to  discharge  to  a  settling 
tank.   From  the  settling  tank  it  is  returned  to  the  washer  via  a  high  pressure 
spray  system.   The  washer  system  could  be  greatly  simplified  by  locating  the 
settling  tanks  and  moving-belt  screens  underneath  the  washers  where  they  could 
receive  the  overflow  by  gravity.   This  would  eliminate  the  sump  pumps,  reduce 
the  floor  space  required  and  would  probably  increase  the  effectiveness  of  the 
settling  tanks  by  equalizing  the  flow  to  them. 

The  redesign  of  the  system  should  include  construction  of  a  second 
prototype,  some  limited  laboratory  testing  to  verify  certain  operating 
characteristics,  and  development  of  a  complete  set  of  plans  and  specifi- 
cations.  These  plans  could  than  be  made  available  to  interested  food 
processors  and  equipment  manufacturers.   The  food  processing  industry  as 
a  whole  is  very  large  and  includes  several  giant  corporations.   Most  food 
processing  plants,  however,  tend  to  operate  in  an  autonomous  fashion,  draw- 
ing little  more  than  administrative  support  from  their  parent  companies. 
Research  and  development  of  needed  machinery  are  pursued  rather  haphazardly, 
usually  on  a  "cut  and  try"  basis.   In  order  for  a  new  system  to  achieve 
maximum  and  speedy  acceptance  by  this  industry,  information  on  it  should 
be  presented  in  the  most  usable  form.   A  processor  given  a  complete  set  of 
plans  and  specifications  for  an  apparatus  is  more  likely  to  build  it  in 
his  own  shop  or  have  it  built  than  one  who  has  to  worry  about  design  detail. 

After  the  second  prototype  is  built  it  should  be  installed  in  a  commer- 
cial processing  plant,  somewhere  in  the  U.  S. ,  and  used  under  normal  operat- 
ing conditions  for  an  extended  period  of  two  to  three  years.   This  would 


provide  a  reference  demonstration  for  other  processors  and  allow  for  refine- 
ments in  design  and  operating  technique. 

Leafy  vegetables  go  directly  from  the  blancher  to  the  cans  in  a  canning 
process.   In  frozen  food  plants,  however,  they  must  be  cooled  before  being 
packaged.   This  is  usually  done  by  fluming  the  product  in  large  volumes  of 
fresh,  cool  water.   The  water  from  this  cooling  process,  or  a  portion  of  it, 
is  then  used  in  the  washers  after  the  cooled  product  is  dewatered.   Because  the 
cooling  water  is  usually  in  excess  of  that  required  by  the  washers  little 
economic  or  environmental  advantage  would  be  gained  by  using  low-water-use 
washers  in  vegetable  freezing  plants  without  using  alternate  means  of  product 
cooling.   Some  devices,  such  as  air  coolers,  are  available,  but  it  appears 
that  further  studies  in  this  area  of  vegetable  processing  are  warranted. 

A  comprehensive  review  of  literature  on  both  combined-  and  unit- 
operation's  effluents  from  fruit  and  vegetable  processing  should  be 
initiated  before  this  literature  becomes  voluminous.   This  review  should 
be  conducted  with  the  object  of  accumulating  known  data  in  condensed  form 
and  "normalizing"  it  to  a  standard  form  of  presentation.   A  corollary 
effort  to  this  review  would  be  the  publication  of  guidelines  for  future 
studies  to  indicate  what  data  should  be  taken  and  how  it  should  be 
expressed.   The  review  and  guidelines  should  be  developed  with  the  view 
of  providing  designers  of  processing  equipment  and  waste  treatment  facil- 
ities with  the  most  useable  data. 


SECTION  4 
PROTOTYPE  WASHER  SYSTEM 


WASHER  DESIGN 

Lopez  (11)  described  the  three  most  common  types  of  leafy  vegetable 
washers  as  the  1)  immersion,  2)  rotary  spray,  and  3)  spray  belt.   Of  these 
the  immersion  (sometimes  called  immersion  drum,  drum,  paddle  wheel  or 
dunker  washer)  is  the  most  popular.   Frey  (8)  (9) ,  in  response  to  industry 
concerns  for  cleaner  product,  made  several  modifications  to  a  conventional 
immersion  washer  and  demonstrated  their  effectiveness  for  removing  insects 
and  grit  from  spinach.   He  also  measured  BOD,  COD,  TS,  SS  and  VSS  of  the 
waste  stream.   The  water-use  rate  in  this  washer  was  approximately  one  gal/ 
lb  of  product,  well  below  the  industry  average.   The  low  levels  of  the  waste 
strength  parameters  indicated  the  feasibility  of  developing  a  washer  proto- 
type system  incorporating  the  principle  of  water  recirculation. 

General  Design  of  Washing  System 

A  full  scale  prototype  of  an  immersion  washing  and  water  recirculating 
system  was  constructed  incorporating  several  of  Frey's  modifications  to  a 
conventional  washer.   It  consisted  of  two,  modified,  leafy-vegetable  immer- 
sion washers  in  series  and  their  respective  settling  tanks  and  moving-belt 
screens  for  cleaning  the  water  in  the  recirculation  process.   Figure  1  shows 
the  arrangement  of  the  system,  as  well  as  product-  and  water-flow  patterns. 
Fresh  makeup  water  was  introduced  into  settling  tank  number  2  during  trials 
in  the  fall  of  1975.   This  arrangement  was  changed  to  apply  the  makeup  water 
as  a  final  spray  on  the  product  leaving  washer  number  2  during  the  spring 
trials  of  1976.   Excess  water  from  settling  tank  number  2  overflowed  into 
settling  tank  number  1.   This  was  the  only  hydraulic  link  between  the  two 
washing  units.   Excess  water  in  settling  tank  1  flowed  to  waste.   Figure 
2  is  an  overhead  view  of  the  washing  system  as  it  was  installed. 

Description  of  a  Washing  Unit 

Each  washer  and  settling  tank  was  constructed  of  11  gage,  type  304, 
stainless  steel  sheets,  with  a  2  x  2  x  1/4  inch  angle-iron  frame  around  the 
top.  The  washer  was  designed  to  wash  approximately  4,000  pounds  of  product 
per  hour  based  on  similar  conventional  washers  at  a  local  processing  plant. 
Each  washer  was  4  feet  wide  and  16  feet  long  with  three  "V"  shaped  sections 
forming  the  bottom  of  the  tank  (Figure  3) .  This  configuration  aided  in  the 
removal  of  grit  when  the  tank  was  drained.  The  tank  was  three  feet  deep  at 
the  deepest  point,  1  foot-10  inches  at  the  shallowest  point,  and  held  688 
gallons  of  water  when  filled  to  the  working  depth  of  2  feet,  7  inches. 


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patterns,  sampling  sites  and  water  flow  meter  locations. 


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Water  was  introduced  from  the  settling  tanks  into  the  washer  at  several 
locations.   There  were  three  banks  of  nozzles  at  the  input  end  of  the  tank, 
one  located  at  the  water  level  and  two  positioned  above  the  incoming  pro- 
duct (Figure  3).   Each  bank  consisted  of  four,  brass,  Flat-Jet  No.  1/2 
P35100  nozzles,  manufactured  by  Spraying  Systems  Company.   They  were  mounted 
on  1-1/4- inch  PVC  pipe  with  split-eyelet  connectors.  Spraying  Systems  No. 
8370A.   These  sprayers  spread  the  incoming  product,  began  the  agitation 
process  to  remove  grit  and  trash,  and  propelled  the  product  toward  the  first 
agitation  drum.   The  entire  spraying  system  was  designed  for  200  gallons  per 
minute  (gpm)  at  a  pressure  of  35  pounds  per  square  inch  (psi) . 

Three  agitation  drums,  or  paddle  wheels,  on  each  washer  served  to 
agitate  the  product  by  alternately  submerging  and  releasing  it  to  remove 
grit  and  trash.   They  were  driven  with  No.  60  roller  chain  at  11  revolutions 
per  minute  (rpm)  by  a  1-horsepower  (hp) ,  3-phase  electric  motor  coupled  to 
a  Winsmith  >D00T  right  angle,  60:1,  speed  reducer.   The  drums  were  1  foot 
11-3/4  inches  in  diameter  and  were  covered  with  16  gage,  3/4-inch  mesh, 
flattened  expanded  stainless  steel  metal  that  allowed  insects  and  leaf 
fragments  to  float  to  the  surface  inside  the  drum  while  the  product  was 
submerged  (Figure  4).   They  each  had  four,  4-inch  fins  around  their  perimeter. 
A  stationary  bank  of  three,  flat-fan,  brass  Vee  Jet  No.  H  1/2  U80100  nozzles, 
manufactured  by  Spraying  Systems  Company  was  positioned  inside  each  drum. 
The  nozzles  were  mounted  with  split-eyelet  connectors  on  a  1-1/4  inch,  PVC 
pipe.   This  pipe  was  inserted  through  a  hollow  hub  of  the  drum  which  located 
the  bank  along  the  axis  of  the  drum  and  thus  allowed  it  to  remain  stationary 
while  the  drum  rotated.   The  nozzle  bank  was  oriented  so  that  the  spray  would 
strike  the  drum  covering  at  the  water  surface  where  the  product  was  released. 
This  served  to  clean  the  drums  during  operation  by  preventing  leaves  from 
becoming  entangled  in  the  expanded  metal  covering.   In  addition  the  spray 
from  these  nozzles  was  another  water  input  to  the  washer  and  an  aid  in  the 
agitation  process.   The  drums  propelled  the  product  through  the  washer  and  on- 
to an  exit  conveyor  (Figures  3,  5).   This  conveyor  was  constructed  of  an  open- 
mesh  belting  made  of  plastic  sections  (manufactured  by  Intralox,  Inc.).   It 
had  flights  every  24  inches  and  was  driven  with  No.  60  roller  chain  by  a 
1/4-hp,  single-phase,  gear  motor  at  a  speed  of  33  feet  per  minute  (f pm) . 
The  conveyor  was  inclined  30°  from  the  horizontal. 

The  agitation  drums  had  a  spoked  construction  on  one  end  (Figure  4)  to 
allow  water  and  trash  collected  inside  the  drum  to  flow  out  through  side 
drains  cut  in  the  washer  tank.   The  side  drains  were  4  1/2  inch  diameter  semi- 
circles with  their  bottom  edges  located  5  inches  below  the  top  of  the  washer 
(at  the  working  depth  of  the  water).    Skimmers  made  of 

3-inch  diameter  stainless  steel  tubing  with  lengthwise  slots  4  feet  long  by 
2  inches  wide  were  installed  directly  behind  each  drum.   They  were  positioned 
to  allow  the  product  to  flow  beneath  them  before  surfacing  after  it  had  been 
submerged  by  the  paddle  wheels.   Their  purpose  was  to  skim  floating  trash 
from  the  water  surface  before  it  could  recontaminate  the  product. 

Water  and  trash  from  the  skimmers  and  side  drains  (Figure  6)  were 
collected  in  a  sump  box,  2  feet  by  2  feet  by  1  foot  4  inches.   A  sump  pump 
(2-hp,  230  volt,  single  phase,  Kenco  No.  34N2  submersible),  with  a  capacity 


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14 


of  14,000  gallons  per  hour  at  15  feet  of  head,  was  used  to  pump  trash  and 
water  from  the  sump  box  to  a  moving-belt  screen  that  was  mounted  on  top  of 
the  settling  tank.   This  pump  was  controlled  by  a  Kenco  Series  112-C12 
Liquid  Level  Control. 

A  gate  valve  was  used  to  regulate  the  flow  from  the  sump  pump  through 
a  3-inch,  PVC  pipe  to  the  filter.   The  moving-belt  screen  was  a  conveyor  (5 
feet  long  by  1  foot  wide)  inclined  at  an  angle  of  16°  from  the  horizontal 
to  prevent  water  from  flowing  off  the  exit  end.   The  belt  was  made  of 
No.  410  Monofilament  Polyester  Screen  manufactured  by  the  Globe  Albany 
Company  and  had  a  permeability  of  600  cubic  feet  per  minute  (cfm)  per 
square  foot  under  a  1/4-inch  head  (Figure  7) .   The  belt  was  chain-driven 
at  47  fpm  by  a  1/4  hp  electric  gear  motor.   Trash  was  carried  away  on  the 
belt  while  the  water  flowed  through  it.   A  3/4-inch  galvanized  pipe,  which 
had  forty-eight  1/16-inch  holes  spaced  1/4- inch  apart  along  its  length, 
was  positioned  under  the  exit  end  of  the  moving-belt  screen.   Compressed  air 
was  directed  through  this  pipe  and  against  the  belt  to  remove  the  trash.   This 
trash  was  collected  in  boxes  placed  at  the  end  of  the  moving-belt  screen 
(Figure  8) . 

Water  flowed  through  the  moving-belt  screen  and  into  a  settling  tank 
where  grit  could  settle  out.   The  tank  was  8-feet  long  by  4-feet  wide  with  a 
4-foot-6  inch  maximum  depth  and  a  3-foot  minimum  depth.   Figure  9  shows  the 
settling  tank  construction  as  well  as  the  direction  of  water  flow  through  it. 
The  baffles  prevented  floating  material  from  getting  to  the  pump.   The  tank 
held  approximately  700  gallons  of  water  and  had  an  overflow  rate  of  2.81  gpm/ 
ft^  based  on  an  assumed  particle  size  of  50  microns  and  a  particle  density  of 
2.65  g/cc  [Metcalf  and  Eddy  (13)]. 

An  Aurora  Model  344  centrifugal  pump,  with  a  3-inch  inlet  and  a  2  1/2- 
inch  outlet,  was  used  to  pump  the  water  from  the  settling  tank  to  the  washer 
spray  nozzles.   The  pump  capacity  was  200  gpm  against  35  psi.   It  was  driven 
by  a  3-phase,  1800  rpm,  7-1/2  hp,  electric  motor  with  a  V-belt  drive. 

A  gate  valve  was  used  to  regulate  the  flow  from  the  settling  tank 
through  a  2-1/2-inch  PVC  pipe  to  the  washer  tank.   The  flow  was  divided  at 
the  washer  and  carried  to  the  nozzle  banks  by  1-1/4-inch  PVC  pipe. 

Motor  starters  for  the  10  electric  motors  in  the  system  were  assembled 
on  a  control  panel,  making  it  possible  for  one  person  to  control  the  entire 
washing  system  from  one  location. 


WATER  FLOW  INSTRUMENTATION 

Five  meters  were  installed  in  the  washing  system  to  monitor  fresh 
water  input,  recirculation  rates,  and  overflow  rates  (Figure  1).   Meters 
and  2  measured  the  recirculation  rates  within  washing  units  1  and  2, 
respectively.   The  meters  used  were  Badger  Model  MLFT,  3- inch  totalizing 
propeller  meters  with  a  normal  operating  range  of  35  to  200  gpm. 


15 


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A.  Water  input 

B.  Water  outlet  to  pump 

C.  Baffles 

D.  Direction  of  flow 

E.  Port  for  draining  tank 

Figure  9:   Prototype  settling  tank 


18 


Flow  meter  3  measured  the  flow  of  fresh  makeup  water  into  the  system. 
A  1-1/2-inch  Badger  Model  SC-ER  totalizing,  disc-type  meter,  with  a  normal 
operating  range  between  5  and  80  gpm,  was  used  to  monitor  this  flow  which 
was  regulated  by  a  gate  valve.   The  makeup  water  was  piped  directly  into 
the  second  settling  tank  from  the  meter  during  the  test  trials  made  in  the 
fall  of  1975.   During  the  spring  trials  of  1976,  the  makeup  water  was  intro- 
duced as  a  spray  through  five  nozzles  (Spraying  Systems  Co.  Flat-Jet  No.  1/2 
P35100)  onto  the  exit  belt  of  washer  2  to  provide  a  final  product  rinse. 
This  was  the  only  modification  made  to  the  experimental  prototype  between 
the  two  seasons. 

The  overflow  from  settling  tank  2  to  settling  tank  1  was  measured  by 
meter  number  4.   This  meter  was  an  0.8-foot  deep,  Plexiglas,  HS  flume  con- 
structed according  to  specifications  in  the  Field  Manual  for  Research  in 
Agricultural  Hydrology  (10).   A  Friez  FW-2,  water  stage  recorder  was  used 
to  continuously  monitor  the  depth  of  water  in  the  flume  (Figure  10) .   For 
details  of  the  construction  and  calibration  of  Plexiglas  HS  flumes,  see 
Robinson  and  Wright  (17) . 

Meter  number  5  measured  the  water  flow  from  settling  tank  No.  1  to  the 
drain.   An  0.8-foot,  HS  flume  and  Friez  recorder  were  also  used  to  monitor 
this  flow  (Figure  11). 


INSTALLATION  AND  MODIFICATIONS 

The  washing  system  was  built  in  the  Agricultural  Engineering  Department 
Laboratory  on  the  campus  of  Virginia  Polytechnic  Institute  and  State  Univer- 
sity (VPI&SU) .   It  was  determined  to  be  operational  and  then  disassembled  and 
transported  to  the  Exmore  Foods,  Inc.,  plant  in  Exmore,  Virginia.   The 
washing  apparatus  was  reassembled  adjacent  to  the  Exmore  plant  for  testing 
at  that  site  (Figure  2) .   Leafy  greens  were  conveyed  out  of  the  plant  to  the 
washers  after  having  passed  over  a  series  of  dry  inspection  belts.  After 
passing  through  the  experimental  washers,  the  product  was  carried  back  into 
the  plant  and  allowed  to  pass  through  the  plant's  conventional  washers.  A 
reversible- feed  belt  was  used  to  carry  the  product  from  the  dry  inspection 
belts  directly  into  the  conventional  washers  when  the  experimental  washers 
were  not  in  use. 

Initial  testing  of  the  washer  system  with  turnip  greens  revealed  that 
the  skimmers  did  not  function  as  anticipated.   The  product  did  not  pass 
under  the  skimmers,  but  collected  on  top  of  them.   A  skimmer  had  been  used 
successfully  by  Frey  (8) ,  but  with  a  considerably  lower  product  flow  rate. 
The  skimmers  were  removed  and  their  drains  sealed  off.   The  remaining  side 
drains,  located  at  the  ends  of  jthe  paddle  wheels,  were  not  large  enough  to 
carry  off  the  flow  introduced  by  the  nozzle  banks,  so  two  modifications 
were  made  to  overcome  this  problem.   Larger  rectangular  side-drains  (4  inches 
high  by  7  inches  wide)  were  cut  at  these  locations  with  the  bottom  of  each 
drain  2  inches  below  the  designed  water  surface  level  of  the  washer.   In 
addition,  the  bank  of  nozzles  closest  to  the  first  drum  was  sealed  off. 
This  latter  modification  was  made  in  order  to  maintain  high  nozzle  pressure 
while  reducing  the  water  recirculation  rate. 

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SECTION  5 
PROCEDURES 


OVERVIEW 

Construction  of  the  prototype  leafy  green's  washer  was  completed  on 
June  20,  1975,  and  attempts  were  made  to  purchase  leafy  vegetables  in  bulk 
quantities  for  laboratory  tests.   Very  dry  weather  in  the  local  area,  how- 
ever, had  shortened  the  spring  harvest  season  considerably.   Packers,  who 
would  normally  have  excess  leafy  greens,  were  importing  product  from  other 
regions  to  meet  their  obligations.   Hence,  testing  was  postponed  until  the 
fall  season  of  1975.   Arrangements  were  made  in  the  interim  to  test  the 
prototype  on  site  at  the  Exmore  Foods  Plant  in  Exmore,  Virginia. 

Testing  the  washer  in  a  commercial  food  plant  had  several  advantages. 
Tests  were  more  realistic  because  the  experimental  equipment  was  subjected 
to  the  same  conditions  under  which  conventional  washers  operate.   The  minimum 
material  needed  for  a  reasonable  test  was  estimated  to  be  25  tons.   Arranging 
for  delivery  of  this  amount  of  material  to  the  laboratory  without  spoilage, 
devising  means  to  correctly  meter  it  into  the  washers  and  disposing  of  it  as 
waste  after  testing  would  have  been  a  formidable  task.   Pre-washing  treatments 
of  product,  such  as  dry  tumbling  and  hand  inspection,  could  also  be  performed 
more  easily  in-plant  than  in  the  laboratory.   A  final  advantage  was  that  the 
experimental  equipment  could  be  tested  in  comparison  with  conventional 
washers.   These  comparison  tests  were  subsequently  arranged  for  the  spring 
season  of  1976  under  an  extension  of  the  original  project. 

Several  difficulties  were  encountered  as  a  result  of  working  under 
commercial  conditions.   Principal  among  these  was  the  distance  to  the  test 
site,  350  miles.   Each  trip  to  collect  data  required  a  minimum  of  three 
days,  two  for  travel  and  one  for  tests.   A  large  volume  of  samples  was 
taken  during  each  trial,  and  it  required  special  packing  to  avoid  deteriora- 
tion during  transport  from  the  plant  back  to  the  laboratory  at  Virginia 
Tech.   For  each  trial,  a  considerable  number  of  small  instruments  and  a 
variety  of  glassware  had  to  be  transported  to  the  plant  and  set  up  in  a 
temporary  lab  on  the  processing  floor  to  supplement  the  company's  laboratory 
facilities.   Finally,  there  was  considerable  difficulty  in  arranging  travel 
schedules  to  coincide  with  the  plant's  processing  schedule,  which  was  very 
unpredictable.   Decisions  to  process  a  certain  vegetable,  dependent  on 
weather  and  several  other  factors,  were  often  made  only  a  few  hours  in 
advance  of  actual  processing.   Decisions  to  terminate  processing  were  often 
more  precipitous,  usually  depending  on  some  factor  that  affected  quality. 


22 


Additional  difficulties,  if  they  can  be  called  that,  included  the  fact 
that  the  investigators  had  no  control  over  the  rate  at  which  product  was 
processed,  its  initial  condition  before  washing,  or  down  time  during  trials. 
Even  input  water  flow  rates  fluctuated  somewhat  due  to  changes  in  operating 
pressures  in  the  plant's  water  system.   Developing  equipment  and  procedures 
to  control  all  of  these  variables  in  a  laboratory  experiment  would,  of 
course,  have  added  a  degree  of  precision  to  the  results,  plus  considerable 
time  and  expense  in  obtaining  them.   This  added  precision,  however,  would 
not  have  offset  the  insight  gained  from  working  under  more  realistic 
conditions. 

Prototype  Installation,  Plant  Layout  and  Conventional  Washers 

The  prototype  washer  system  was  installed  adjacent  to  the  Exmore  Foods 
Plant  in  Exmore,  Virginia  during  the  week  of  August  4,  1975  as  described  in 
Section  4.   Exmore  Foods  has  two  leafy  vegetable  processing  lines,  an  east 
line  and  a  west  line.   The  prototype  was  located  so  that  it  could  operate  in 
series  with  the  conventional  washers  of  the  west  line  or,  when  not  in  use, 
could  be  bypassed  (Figure  12) . 

The  east  and  west  conventional  processing  lines  had  two  paddle  wheel 
washers  each  in  series  (Figure  12)  followed  by  a  combined  paddle  wheel 
washer/pre-blancher,  and  then  a  blancher.   The  washers  were  three  and  one- 
half  feet  wide,  approximately  eighteen  feet  long,  and  three  feet  deep  at  the 
deepest  point.   There  were  four  paddle  wheels  in  each  washer  for  propelling 
the  product  through  the  washer.   Product  from  the  blancher  was  cooled  and 
transported  in  a  cooling  flume  fed  by  fresh  water.   Dewatering  and  recircula- 
tion of  the  cooling  flume  water  provided  all  the  input  water  for  the  washers 
on  the  west  line.   The  water  input  was  at  the  head  of  each  washer  through  a 
perforated  pipe.   The  dewatering  of  the  cooling  flume  water  on  the  east  line 
provided  only  a  portion  of  the  input  water.   The  majority  of  this  water  was 
fresh,  piped  into  the  bottom  of  the  washers.   All  the  overflow  from  the 
washers  was  wasted  to  an  open  channel  floor  drain. 

Overflow  from  the  conventional  washers  was  measured  with  HS  flumes  and 
water  stage  recorders  like  those  used  in  the  prototype  system.   An  attempt 
was  made  to  measure  input  water,  which  was  under  pressure,  with  propeller- 
type  totalizing  water  meters.   These  meters  soon  became  inoperative  because 
some  large,  vegetable  particles  were  being  pumped  from  the  dewaterers  to  the 
washers.   However,  an  accurate  estimate  of  the  input  water,  it  was  reasoned, 
could  be  made  by  measuring  the  amounts  of  effluent  water  from  each  unit  and 
using  the  data  in  water  carried  off  by  the  product  available  from  the  proto- 
type trials. 

Summary  of  Trials 

Tables  1  and  2  summarize  the  trials  that  were  made  during  the  fall  and 
spring  processing  seasons.   A  total  of  35,200  kilograms  of  product  was 
processed  through  the  prototype  in  27  hours  of  actual  operation  during  the 
fall  and  31,500  kilograms  in  24.4  hours  during  the  spring.   A  total  of 
16,500  kilograms  was  processed  in  11.7  hours  through  the  east  conventional 
line  and  21,200  kilograms  in  15.3  hours  through  the  west  conventional  line 

23 


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Figure  12:   Schematic  showing  water  and  product  sampling  sites  for 
comparative  study  of  new  vs.  conventional  leafy  greens 
washing  systems  at  Exmore  Foods,  Exmore,  Va.  Circled 
numbers  refer  to  water  and/or  product  sampling  sites. 


24 


TABLE  1.   SUMMARY  OF  INFORMATION  FOR  TRIALS  OF  PROTOTYPE  WASHER  SYSTEM 

DURING  THE  FALL  SEASON  OF  1975 


Trial 

Date 

Product 

Total  Operating 
Time  (hrs) 

Total 
of  Pi 

* 

Fresh  Weight 

roduct  Washed 
(kg) 

1 

10/24/75 

Collards 

3.80 

** 

4418 

2 

11/4/75 

Collards 

5.78 

9975 

3 

11/20/75 

Collards 

6.68 

8866 

A 

12/1/75 

Collards 

6.68 

7945 

5 

12/15/75 

Spinach 

4.05 

3989 

** 


Fresh  weight  through  washer  system  obtained  from  weight  of  product 
packaged  adjusted  by  determinations  of  relative  moisture  contents 
of  incoming  and  packaged  product.   These  figures  approximate  raw 
product  entering  first  washer. 

Product  samples  for  moisture  content  analysis  were  damaged  in 
transport  to  laboratory.   Estimate  was  made  from  data  from  other 
collard  trials. 


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26 


during  the  spring.   The  amounts  of  product  quoted  here  (and  in  the  last 
columns  of  Tables  1  and  2)  are  fresh  (i.e.  raw)  weights  as  delivered  into 
the  first  washer  from  the  dry  inspection  belts.   Each  trial  consisted  of  a 
complete  or  partial  eight  or  nine  hour  shift.   Down  time  and  breaks  were 
substracted  from  total  time. 

The  company  packaged  five  different  varieties  of  leafy  greens  during 
the  fall  season,  the  largest  volume  of  which  was  collards.   Consequently, 
travel  schedules  of  the  investigators  coincided  with  collard  processing 
four  out  of  five  trials.   The  leaves  of  this  variety  tended  to  be  large, 
very  mature,  relatively  clean,  and  easy  to  wash.   Spinach  was  processed 
during  the  fifth  trial.   It  was  not  as  clean  as  the  collards,  and  the 
leaves  tended  to  be  small  and  immature. 

Initial  plans  for  the  spring  season  included  dual  trials  with  the 
prototype  on  the  west  line  running  simultaneously  with  the  conventional 
east  line.   Unfortunately,  there  were  only  a  few  days  early  in  the  season 
when  both  lines  were  processing  the  same  product,  and  travel  to  the  plant 
could  not  be  arranged  at  those  times.   As  a  best  alternative,  the  prototype 
system  was  tested  during  the  day  shift  (8:00  a.m.  to  5:00  p.m.),  and  data 
were  taken  on  one  of  the  conventional  lines  the  same  night  for  the  first 
half  of  the  night  shift  (6:30  p.m.  to  11:00  p.m.).   Clean-up  and  sample 
preparation  were  usually  complete  by  12:30  a.m.   Material  processed  during 
a  given  day  usually  came  from  the  same  field,  so  comparisons  between  the 
prototype  and  conventional  lines  could  be  made.   Taking  data  on  the  con- 
ventional washers  for  complete  shifts  was  not  necessary  because  the  water 
was  not  recirculated  and,  thence,  effluent  characteristics  and  product 
quality  parameters  were  not  time  dependent.   Four  trials  were  made  in  this 
manner. 

Data  were  taken  on  conventional  lines  alone  during  trials  2  and  3.   The 
west  line,  where  the  prototype  was  located,  was  not  operated  on  the  day  of 
trial  2.   Plant  operations  were  precipitously  curtailed  on  the  day  of  trial 
3  by  a  deterioration  in  the  quality  of  locally  grown  product.   Only  one 
large  truck  load  of  spinach,  purchased  in  a  neighboring  state,  was  processed. 
Consequently,  a  decision  was  made  to  take  data  on  the  conventional  washers 
of  the  west  line  because  data  for  spinach  washing  with  the  prototype  were 
already  available.   Quality  of  the  spinach  processed  in  the  spring  varied 
consider ablly — from  prime  (trial  3)  to  overly  mature  (trials  1  and  2)  and 
from  very  clean  (trial  3)  to  very  dirty  because  of  sprinkler  irrigation  in 
the  field  (trial  2).   The  variation  in  quality  of  turnip  greens,  although 
great,  was  much  less  than  spinach.   Those  in  trial  4  were  average  in  quality 
and  cleanliness,  in  trial  5  overly  mature  and  clean,  and  in  trial  6  good 
quality  and  dirty. 

SPECIFIC  PROCEDURES 

The  following  sections  outline,  in  order,  (1)  water  and  product  sampling 
sites,  (2)  typical  procedures  that  were  followed  for  a  trial  during  the  fall 
season,  (3)  modifications  of  those  procedures  for  the  spring  trials  and 
(4)  analytical  procedures. 

27 


Sampling  Sites 

Water  and  product  sampling  sites  were  selected  so  that  the  effect  of 
each  major  component  of  the  system  could  be  evaluated  for  each  parameter 
measured.   Product  samples  were  taken  on  the  feed  conveyor  to  the  first 
washer,  the  exit  conveyor  for  the  first  washer  and  the  exit  conveyor  for 
the  second  washer  in  each  case  (product  sampling  sites  1,  2,  3,  Figure  1 
for  the  prototype;  sites  7,  8,  10,  for  the  east  conventional  line  and  12, 
13,  15  for  the  west  conventional  line,  Figure  12).   Packaged  product  samples 
were  taken  at  the  end  of  the  respective  line  in  each  trial  (sites  11,  16, 
Figure  12) . 

Water  sampling  sites  for  the  prototype  are  depicted  in  Figure  1.   Sites  1 
and  3  were  the  spray  nozzles  at  the  head  end  of  washers  1  and  2,  respectively, 
sites  2  and  4  the  sump  boxes  that  collected  the  total  flow  from  each  washer, 
and  sites  5  and  6  the  input  ends  of  the  settling  tanks.   Hence,  for  example, 
difference  in  samples  between  sites  1  and  2  measured  the  effect  of  washer  1, 
between  sites  2  and  6  the  effect  of  the  sump  pump  and  filter  belt,  and  between 
sites  6  and  1  the  effect  of  the  settling  tank.   Similarly,  water  samples  from 
the  conventional  line  were  taken  at  entrance  end  of  each  washer  and  from  the 
overflow  at  the  exit  end  of  each  washer.   (Sites  7,  8,  9,  10,  12,  13,  14,  15, 
Figure  12) . 


Typical  Day  -  Fall  Trials 

The  sequence  of  events  for  each  trial,  of  course,  varied, 
outlines  a  typical  work  day  during  the  trials. 


The  following 


Time 


Event 


1)   Night  prior  to  trial 


a)   Laboratory  set  up  on  location. 


b)  Water  quality  instruments  and  flow 
meters  calibrated. 

c)  BOD  dilution  water  prepared  and  aerated. 

d)  Prototype  washers  and  settling  tanks 
rinsed  and  filled. 


2)   Morning  of  trial,  before 
processing 


a)  Washer  and  instruments  turned  on. 

b)  Flow  rates  set  on  meters  1,  2,  and  3. 
(See  Figure  1). 


c)  Recorders  started  on  meters  4  and  5. 

d)  Sodium  sulfite  prepared  to  neutralize 
chlorine  in  BOD  and  bacteriological 
samples. 


28 


Time 

3)  Beginning  of  trial 

4)  After  15  minutes 
operation 


Event 

a)   Start-up  time  recorded. 

a)   Grab  samples  of  product  taken  for  moist- 
ure content,  bacterial  counts  and  insect 
counts. 


5)   After  each  hour  of 

operating  time  (exclusive 
of  breaks  and  dovmtime)  . 


b)  Grab  samples  of  water  taken  for  biochemical 
oxygen  demand  (BOD) ,  chemical  oxygen  demand 
(COD),  color,  turbidity,  chlorine  residual, 
total  suspended  solids  (TSS)  ,  volatile 
suspended  solids  (VSS) ,  conductivity  and 
pH. 

c)  Grab  samples  of  water  collected  in  sterile 
bottles  for  total  plate  counts  and  con- 
form counts  (repeated  at  middle  and  end 

of  trial) . 

a)  Same  as  4,  a  and  b. 

b)  Number  of  packages  of  product  processed 
recorded. 


c)   Flow  meters  1,  2  and  3  checked  with  stop 
watch. 


6)   Between  samplings 


a)   Chlorine  residual  determined- 
fa)   bod's  set  up  and  placed  in  a  low 

temperature.  Precision  Model  815 

incubator. 


c)  Conductivity,  color,  turbidity,  and  pH 
determined. 

d)  Samples  for  TSS  and  VSS  filtered,  cruci- 
bles and  filters  prepared  for  shipment 
to  Virginia  Polytechnic  Institute  and 
State  University  laboratory  in  Blacksburg, 
Virginia. 

e)  Remaining  water  preserved  with  acid  for 
return  to  Blacksburg  for  COD  determin- 
ation. 

f)  Water  samples  for  bacteriological 
analysis  plated  and  incubated  at  35°C. 

g)  Product  samples  weighed  and  frozen  in 
plastic  bags. 


29 


Time  Event 

7)  After  trial  a)  Trash  collected  and  weighed. 

b)  Tanks  drained. 

c)  Grit  collected. 

8)  Day  after  trial  a)  Product  samples  packed  in  ice. 

b)  BOD  bottles  placed  in  cartons  to 
maintain  approximate  temperature  of 
20°C  during  transport. 

c)  Bateriological  samples  with  sufficient 
growth  were  counted.   Others  were  packed 
for  transport. 

d)  Samples  and  equipment  transported  to 
Blacksburg. 

9)  Week  following  trial  (in    a)   Moisture  contents,  grit  particle  sizes, 
V.P.I.  &  S.U.  laboratory)       COD's,  TSS,  VSS ,  BOD^'s,  BOD„q's,  insect 

counts  and  bacterial  counts  determined. 

b)   Preparations  made  for  next  trial. 

Variations  Between  Spring  and  Fall  Trials 

Procedures  for  the  spring  trials  were  essentially  the  same  as  those  for 
the  fall  with  the  following  exceptions: 

(1)  Product  samples  were  taken  for  grit  analysis  in  addition  to  samples 
taken  for  moisture  content,  bacterial  counts,  and  insect  counts.  Samples  for 
grit  analysis  were  taken  at  two-hour  intervals  of  operating  time  rather  than 
every  hour.  Product  samples  for  grit  analyses  were  hand  washed  and  the  wash 
water  filtered  between  sampling  periods  for  later  determination  of  suspended 
solids. 

(2)  Water  samples  were  taken  for  analyses  of  COD,  TSS,  VSS,  chlorine 
residual,  pH,  and  pesticides  both  spring  and  fall,  but  BOD,  color,  turbidity, 
and  conductivity  were  not  measured  in  the  spring  trials.   Samples  for 
pesticide  analysis  were  taken  twice  during  each  trial,  mid-way  and  end,  at 
the  outlet  of  the  first  washer  of  the  prototype  only  (site  2,  Figure  1). 
During  the  fall  trials  water  samples  were  taken  at  the  input  end  of  each 
settling  tank  (sites  5  and  6,  Figure  1)  by  dipping  the  container  into  the 
surface  of  the  water.   Some  rapidly  settling  solids  may  have  been  lost  by 
this  technique.   In  the  spring  trials  these  samples  were  taken  by  holding 

the  container  directly  under  the  moving  screen  belt  to  catch  the  water  before 
it  entered  the  settling  tank. 


30 


(3)  Water  and  product  sampling  for  the  conventional  washers  were 
essentially  the  same  as  those  for  the  prototype  except  for  sampling 
associated  with  the  settling  tanks.   An  attempt  was  made  to  get  a  quanti- 
tative analysis  of  grit  accumulated  in  the  conventional  washers,  but  this 
could  not  be  accomplished  without  interfering  with  company  personnel  in 
their  clean-up  procedures.   One  set  of  soil  samples,  however,  was  obtained 
for  particle  size  analysis. 

Analytical  Procedures 

Following  is  a  brief  summary  of  the  procedures  used  in  analyzing  samples 
for  each  type  of  data  taken  during  the  investigation. 

Water  Flow  Rates  — 

Totalizing  water  meters  on  the  prototype  were  read  at  the  beginning  of 
each  trial  and  hourly  thereafter  to  obtain  flow  rates  as  a  function  of  time. 
Depth  of  water  flow  through  the  HS  flumes  was  continuously  recorded  on  strip 
chart  recorders.   Flow  rates,  as  they  varied  with  time,  were  later  calculated 
using  these  recordings  and  the  calibration  curve  of  flow  vs.  depth  that  had 
been  developed  for  the  flumes  (17). 

Product  Flow  Rates  -  - 

Product  samples  for  moisture  content  determination  were  taken  from  input 
conveyors,  weighed,  sealed  in  plastic  bags  and  frozen  for  transport.   Sample 
packages  of  product  from  the  end  of  the  processing  line  were  taken  simul- 
taneously and  frozen  for  transport.   At  the  lab,  these  samples  were  dried  at 
105°C  for  24  hours  in  a  forced  convective  oven,  and  moisture  contents  were 
calculated  on  a  wet  basis.   The  relative  moisture  contents  of  input  and 
output  product  and  the  package  counts  of  output  product  were  then  used  to 
calculate  the  rate  of  fresh  product  input  to  the  washers.   This  was  a 
better  measure  of  input  product  than  total  field  weight  because  considerable 
material  was  lost  from  the  dry  tumbler  and  the  dry  inspection  belts  ahead  of 
the  washers  and  very  little  was  removed  by  subsequent  inspections  between 
the  blanchers  and  packaging. 

Grit  Accumulation  -  - 

The  washers  and  settling  tanks  of  the  prototype  were  drained  after 
each  trial  and  the  volume  of  grit  on  the  bottom  of  each  tank  was  measured. 
Some  grit  was  inevitably  lost  while  the  tanks  were  draining.   An  attempt  to 
recover  this  loss  was  made  by  filtering  the  water  as  it  flowed  from  the 
tank.   These  filters  were  effective  in  trapping  the  larger  sand  particles, 
but  allowed  some  of  the  smaller  silt  and  clay  particles  (those  particles 
with  a  diameter  of  less  than  50  microns)  to  be  lost.   Grit  loss  in  the 
washer  tanks  was  minimized  by  draining  the  tank  through  the  port  farthest 
from  the  incoming  product,  where  the  grit  accumulation  was  lowest.   At  the 
end  of  trial  5  in  the  fall,  a  submersible  sump  pump  was  used  to  empty  the 
two  settling  tanks.   This  technique  was  used  to  empty  the  washers  and 
settling  tanks  in  the  spring  trials. 


31 


After  the  volume  of  grit  had  been  determined,  samples  were  taken  from 
each  of  the  three  bottom  sections  of  the  washers  and  from  the  settling 
tanks.   At  the  laboratory,  they  were  dried  in  a  forced  convective  oven  at 
lOS^C  for  24  hours.   A  soil  particle  size  analysis  was  then  performed 
using  standard  hydrometer  methods  (5)  for  particles  in  the  range  below 
50  microns  and  standard  sieve  analysis  (3)  for  larger  particles. 

Trash  Accumulation  -  - 

The  trash  collectors  for  the  prototype  were  emptied  at  the  end  of  each 
trial  and  their  contents  weighed.   Samples  from  each  moving-belt  screen  were 
taken,  weighed,  and  frozen  for  transport  to  the  laboratory.   There,  they  were 
dried  by  the  same  procedure  as  used  for  the  product,  and  moisture  contents 
were  calculated.   These  figures  were  compared  with  those  for  the  incoming 
product  to  determine  the  weight  of  trash  collected  when  corrected  to  the 
moisture  content  of  the  incoming  product. 

Water  Sample  Analyses  -  - 

Following  is  a  summary  of  methods  used  in  analyzing  water  samples  taken 
during  the  trials. 

Chlorine  residual  —  The  total  chlorine  residual  was  determined 
amperometrically  as  described  in  Standard  Methods  for  the  Examination  of 
Water  and  Waste  Water  (Sec.  114B)  (19)  with  a  Fischer-Porter  amperometric 
titrator. 

pH  —  The  pH  was  determined  as  described  in  Standard  Methods  (Sec.  144A) 
(19)  with  a  Corning  Model  F  pH  Meter  in  the  fall,  with  a  Fisher  Accumet  Model 
230  in  the  spring. 

BOD;.  —  The  BOD_  was  determined  as  described  in  Standard  Methods  (Sec. 

219)  (19) .   Sodium  sulfite  was  added  stoichiometrically  to  neutralize 

the  chlorine  residual,  which,  if  not  treated,  could  kill  the  microorganisms 
present  in  the  sample.   Experiments  were  conducted  to  determine  the  difference 
between  BOD_  of  a  seeded  and  unseeded  sample.   No  difference  was  detected,  so 
the  samples  were  not  seeded. 

Color  —  True  color  was  determined  by  filtering  a  portion  of  water 
sample  through  a  Reeve  Angei  glass- fiber  filter  and  determining  the  optical 
density  on  a  Klett-Summerson  Photometer,  using  a  #42  (blue)  filter. 

Turbidity  —  Turbidity  was  determined  by  measuring  the  optical  density 
of  a  small  portion  of  water  sample  with  a  Klett-Summerson  Photometer,  using 
a  #42  (blue)  filter. 

Solids  —  Total  suspended  and  volatile  suspended  solids  were  determined 
according  to  Standard  Methods  (Sec.  148B  and  244D,  respectively)  (19). 

COD  —  Water  samples  for  COD  were  acid-fixed  (ph  'v  2.0)  for  preservation 
until  analyzed.   Determinations  were  made  in  the  laboratory  as  described  in 
Standard  Methods  (Sec.  220)  (19). 

32 


Bacterial  counts  —  The  total  plate  count  was  determined  according  to 
Standard  Methods  (Sec.  660)  (19)  except  during  trials  3,  4  and  5  during  the 
fall  and  trials  1,  2  and  3  during  the  spring  when  the  streak-plate  technique 
was  used  instead  of  the  pour-plate  technique. 

In  addition  to  total  plate  counts,  a  non-specific  coliform  count  was 
made  in  the  fall  trials  using  desoxycholase  agar,  a  selective  medium  for 
conforms.   The  pour-plate  technique  was  used  during  trials  1  and  2  and 
the  streak-plate  technique  was  used  during  trials  3,  4  and  5.   All  colonies 
growing  on  the  media  were  counted. 

Pesticide  Analyses  —  Pesticide  analyses  were  made  on  water  samples 
taken  from  the  first  washer  in  the  prototype  during  the  spring  trials.   The 
samples  were  examined  only  for  organophosphorus  pesticides,  as  they  were  the 
only  ones  applied  to  the  crops  during  the  growing  season.   Phosdrin  was  the 
principal  one  in  use  at  the  time  of  the  study.   Methods  of  analyses  were 
EPA-approved  (Federal  Register  38,  No.  85,  Part  II,  Nov.  28,  1973),  gas 
chromatrographic  analyses  of  solvent  extracts  of  the  samples.   These  were 
conducted  in  the  Department  of  Biochemistry  Pesticide  Analysis  laboratory. 

Product  Sample  Analyses  — 

Following  is  a  summary  of  methods  used  in  analyzing  product  samples 
during  the  trials. 

Bacterial  counts  —  The  method  used  for  determining  the  total  plate 
count  of  the  product  was  that  used  by  technicians  at  Exmore  Foods.   Eleven 
grams  of  product  were  placed  in  a  bottle  containing  ninety  milliliters  of 
sterile  dilution  water.   From  this  bottle,  a  series  of  dilutions  was  made 
and  plated  out  on  Total  Plate  Count  agar.   The  test  proceeded  as  described 
in  Standard  Methods  (Sec.  660)  (19).   Coliforms  were  determined  on  several 
occasions  (but  not  routinely)  by  plating  aliquots  of  the  water  on  desoxycho- 
late  agar. 

Insect  counts  —  Insect  counts  on  product  samples  were  made  using  the 
gasoline  extraction  method  described  by  Townsend  et  al.  (20). 

Grit  on  product  —  A  simple  hand  washing  test  was  devised  to  determine 
the  amount  of  grit  on  the  product  at  each  product  sampling  site.   Duplicate, 
1000-gram  samples  of  product  from  each  site  were  agitated  1-2  minutes  by 
hand  in  containers  with  15  liters  of  water.   Product  was  separated  from  the 
water  with  a  large-mesh  screen  and  an  aliquot  of  500  ml.  from  each  water 
sample  was  filtered  for  solids  determination. 


33 


SECTION  6 
RESULTS  AND  DISCUSSION 


OPERATING  PARAMETERS 

The  following  is  concerned  with  those  quantities  measured  during  the 
trials  that  could  be  considered  operating  parameters,  i.e.,  those  things 
that  were  either  under  the  control  of  or  required  action  by  personnel 
operating  the  systems.   They  include  water  and  product  flow  rates  for  both 
the  prototype  and  conventional  systems,  and  grit  and  trash  accumulations 
in  the  prototype  system. 

Water  Flow  Rates 

Tables  3  and  4  summarize  the  water  flow  rates  used  in  the  prototype 
during  the  fall  and  spring  trials,  respectively.   Figures  13  and  14  are 
examples  of  the  water  flows  in  the  prototype  for  the  fall  and  spring  trials. 
Meters  1  and  2  in  each  graph  represent  the  recirculation  rates  for  washers  1 
and  2,  respectively.   Meter  3  is  the  input  of  fresh  water  to  the  system; 
meter  4,  the  flow  from  settling  tank  2  to  settling  tank  1;  and  meter  5,  the 
overflow  to  waste  from  settling  tank  1  (refer  to  Figure  1).   All  tables  and 
graphs  relate  only  to  water  flows  during  actual  processing  time.   Meters  1,  2, 
and  3  were  totalizing  meters  and  the  graphs  were  plotted  from  readings  taken 
at  timed  intervals.   Meters  4  and  5  were  open  channel  HS  flumes  with  water 
level  strip  chart  recorders.   Data  for  the  graphs  was  taken  from  the  strip 
charts  at  15  minute  intervals.   Graphs  for  the  spring  trials  were  plotted  by 
hand  (Figures  13,  A-1  through  A-4) .   Graphs  for  the  spring  trials  were  taken 
from  computer  plots  (Figures  14,  A-5  through  A-12)  . 

Recirculation  rates  in  the  washers  varied  somewhat  but  were  generally 
maintained  near  404  S,/min  (107  gal/min).   A  recirculation  rate  of  530  Jl/min 
(140  gal/min)  in  washer  1  was  tried  for  a  few  hours  in  one  trial  (trial  5, 
fall.  Figure  A-4) ,  but  it  was  discovered  that  the  control  system  on  the 
sump  pump  did  not  react  fast  enough  to  handle  this  flow. 

Fresh  water  to  the  system  was  introduced  into  the  second  settling  tank 
during  the  fall  trials  (meter  3,  Figure  1).   No  attempt  was  purposely  made 
to  vary  this  flow,  maintained  as  near  the  average  of  66.8  Ji/min  (17.7  gal/ 
min)  as  fluctuations  in  the  plant  water  pressure  would  permit.   Fresh  water 
was  introduced  as  a  final  rinse  spray  on  the  produce  discharge  belt  of 
washer  2  during  the  spring  trials.   In  trials  4,  5,  and  6  (Figures  A-5,  A-6 , 
A-7)  this  rate  was  increased  with  each  trial  [52.9,  71.3,  94.8  fc/min  (respec- 
tively, 14.0,  18.8,  25.0  gal/min)].   During  the  fall  trials,  the  input 
water  was  turned  off  during  periods  of  down  time.   This  technique  was  tried 

34 


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Figure  13:   Water  flow  rates  vs.  operating  tlae,  trial  ]  ,■  Fall, 
1975,  when  processing  collards  with  prototype 
syscen.   Refer  to  Figure  1  for  meter  locations. 


Kigurp  14:  U.iter  flow  r.ntt-s  v^.  o|>crt.it  1  iig  tine,  trial  I.  •^priiiR, 
1476,  wlien  processing  spinanrli  '.Itli  protntypo  Rvstctn. 
Refer  to  Figure  I  for  meter  loi.iiion^. 


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OPERATING  TIME  (hrs.) 


Figure  15.   Water  owerflc«  rates  Iron  conventional  washers 
operating  time,  trial  1.  spring,  1976,  when 
processing  spinach  on  the  east  line. 


37 


during  trial  1  of  the  spring  trials,  but  the  amount  of  water  carried  off 
by  the  product  was  such  that  there  were  periods  of  time  after  breaks  when 
the  overflow  to  waste  and  the  overflow  between  settling  tanks  was  reduced 
to  zero  (Figure  14) .   In  trial  4  (Figure  A-5)  the  input  was  left  partially 
open  during  breaks.   Again,  however,  there  were  periods  of  no  overflow  to 
waste.   This  was  due  to  water  carried  off  by  the  product  plus  the  low  rate 
of  fresh  water  input  (52.9  Jl/min)  .   On  subsequent  trials  the  fresh  water  was 
left  on  during  breaks  which  ultimately  had  the  effect  of  reducing  the  con- 
centration of  the  waste  components  used  as  measures  of  water  quality. 

Differences  between  the  flow  rates  at  meters  3  and  5  represent  the  water 
being  carried  from  the  system  on  the  product.   Differences  between  meters 
4  and  5  represent  water  carried  from  washer  1  into  washer  2  by  the  product, 
and  differences  between  meters  3  and  4  represent  additional  wetting  of  the 
product  in  washer  2.   As  expected,  the  amount  of  additional  wetting  of  the 
product  in  washer  2  was  relatively  slight  in  trials  1  and  2  during  the  fall 
(Figures  13  and  A-1) .   However,  this  trend  was  not  observed  in  the  remainder 
of  the  fall  and  spring  trials.   This  additional  wetting  may  have  been 
influenced  by  a  number  of  factors  including  the  age  and  variety  of  product. 
A  particularly  interesting  phenomenon  was  the  build-up  of  a  soap-like  foam 
on  the  surface  of  the  water  in  the  settling  tanks  and  washers.   It  resulted, 
no  doubt,  from  the  surfactant  action  of  organic  matter  leached  from  the  greens. 

Table  5  is  a  summary  of  the  water  flow  data  for  the  conventional  washers, 
and  Figure  15  is  an  example  graph  (trial  1,  spring).   Graphs  of  the  flows  in 
other  trials  are  included  in  Figures  A-8  through  A-12 ,  Appendix  A. 

The  flow  rate  of  water  to  the  conventional  washers  was  left  entirely  to 
the  judgement  of  the  line  foreman  during  each  shift.   More  or  less  water  was 
used  in  each  washer  based  on  his  judgement  and  experience.   The  graphs  show 
considerable  variations  in  flow  that  did  not  always  appear  to  be  related  to 
initial  product  quality  or  product  flow  rate.   One  mechanical  influence  on 
the  east  line  (trials  1  and  2,  Figures  15  and  A-8)  was  the  inefficiency  of 
the  cooling  flume  dewaterer,  requiring  that  most  of  the  input  water  to 
these  washers  be  fresh.   Consequently,  water  use  on  this  line  tended  to  be 
minimized,  especially  in  washer  2.   Conversely,  the  dewaterer  on  the  west 
line  (trials  4  through  6,  Figures  A-9  through  A-12)  worked  well,  and  all  of 
the  input  to  these  washers  was  recirculated  product  cooling  water,  used 
unstintingly.   In  most,  but  not  all,  cases  more  water  was  used  in  the  first 
conventional  washer  in  a  line  than  in  the  second.   It  seems  obvious  that 
some  means  of  cooling  blanched  product  other  than  with  large  quantities  of 
fresh  water  would  be  necessary  in  order  for  a  company  to  take  maximum 
advantage  of  a  water-conserving  washing  system. 

Several  other  observations  can  be  made  from  the  water  flow  data.  1) 
Though  the  number  of  trials  for  each  product  are  few,  there  is  an  indication 
that  different  varieties  of  greens  tend  to  carry  away  different  amounts  of 
water  from  a  washing  process.   Average  values,  in  order,  are:   collards  - 
1.79  Jl/kg  (0.22  gal/lb),  turnip  greens  -  2.40  Jl/kg  (0.29  gal/lb),  and 
spinach  -  2.78  A/kg  (0.33  gal/lb).   These  figures,  on  a  relative  basis,  are 
consistent  with  expectations  based  on  qualitative  evaluations  of  leaf  sur- 
faces; i.e.,  collards  have  a  waxy,  smooth  surface  compared  to  spinach. 

38 


TABLE  5.   AVERAGE  WATER  USE  DATA  FOR  CONVENTIONAL  LEAFY 
VEGETABLE  WASHERS  DURING  SPRING  TRIALS.  1976 


Trial   ^  ^ 
XT      Date 
No. 

Product 
and 
Line 

Input  tot 

Two 
Washers 
il/min) 

Output  From 
Two 
Washers 
(Jl/min) 

Input 
Water/ 
Productf 
(^/kg) 

Water* 
Carried  Out 
On  Productf 

()l/kg) 

1    4/22/76 

Spinach(E) 

380.3 

328.3 

20.3 

2.78 

2    5/12/76 

Spinach(E) 

179.0 

107.2 

6.9 

2.78 

3    5/21/76 

Spinach (W) 

341.5 

266.3 

12.6 

2.78 

4    6/4/76 

Turnip 
Greens  (W) 

461.1 

405.9 

20.0 

2.40 

5    6/10/76 

Turnip 
Greens  (W) 

455.9 

396.6 

18.4 

2.40 

6    6/11/76 

Turnip 
Greens  (W) 

438.6 

393.0 

23.1 

2.40 

Averages 

376 
(99  gal /rain) 

316 
(84  gal/min)  (2 

16.9 
.02  gal/lb) 

*    Estimated  from  average  values  determined  in  prototype  trials. 

t    Calculated  from  output  data  and  estimated  values  of  water  carried  out 
on  product. 

(E)   =  East  conventional  line. 

(W)   =  West  conventional  line. 

+    Fresh  (raw)  product  entering  first  washer  from  dry  inspection  belts 
(Figure  12) . 


39 


2)  The  water  input  to  two  conventional  washers  averaged  5.2  times  that  of  the 
prototype  system,  the  water  output  or  waste  stream  12.7  times  that  of  the 
prototype.   3)  Average  fresh  water  input  for  the  conventional  washers  was 
16.9  Ji/kg  (2.02  gal/lb)  vs.  3.43  J!,/kg  (0.41  gal/lb)  for  the  prototype,  a 
ratio  of  5:1. 

Product  Flow  Rates 

Several  different  sizes  of  packages  were  used  at  the  Exmore  plant. 
Retail  packages  for  collards  and  turnip  greens  were  283  g  nominal  net  weight 
(10  oz)  and  those  for  spinach  were  340  g  (12  oz) .   In  some  cases  diced 
turnip  roots  were  included  with  the  greens  (8  oz  of  greens,  2  oz  of  roots). 
The  weight  of  roots  was  accounted  for  when  analyzing  the  moisture  content  of 
samples,  and  only  the  greens  processed  are  reported  here.   These  packages 
were  counted  with  an  electronic  counter  located  immediately  following  the 
packaging  machine.   Some  product  was  packed  in  6.8  kg  (15  lb)  trays  for 
institutional  packs  and  some  on  open  trays  in  lots  of  181  kg  (400  lb)  for 
bulk  freezing  and  storage.   The  number  of  these  units  was  recorded  as  they 
were  put  into  the  freezer. 

Figures  16  and  17  are  examples  of  the  instantaneous  flow  rate  and  cumu- 
lative flow  of  fresh  product  into  the  washers  versus  operating  time.   Figures 
A-13  through  A- 30  depict  flows  during  other  trials.   Initially,  it  was 
assumed  that  the  flow  of  material  through  the  system  would  be  rather  uniform. 
Consequently,  only  the  total  product  processed  was  recorded  during  trial  1 
in  the  fall.   During  this  trial  it  became  obvious  that  input  to  the  system 
was  very  erratic.   Inputs  to  the  prototype  system  ranged  from  456  to  2251 
kg/hr  (1105  to  4963  Ibs/hr) ;  for  the  conventional  washers  459  to  3330  kg/hr 
(1012  to  7341  Ib/hr) .   These  wide  fluctuations  in  input  rates  undoubtedly 
affected  the  quality  of  the  product.   An  obvious  means  of  improving  washing 
quality,  and  perhaps  increasing  the  average  processing  rate,  would  be  to 
devise  a  means  of  metering  the  product  more  evenly  into  the  washers. 

Tables  6,  7,  and  8  are  summaries  of  the  product  data.   The  average  rate 
of  fresh  product  input  into  the  washers  for  all  trials  was  1324  kg/hr  (2918 
Ib/hr) ,  and  the  average  rate  of  output  from  processing  was  1808  kg/hr  (3985 
Ib/hr) ,  for  an  output/input  ratio  of  1.37.   A  limited  number  of  tests  during 
the  fall  trails  indicated  that  very  little  water  was  absorbed  in  the  washing 
process.   It  is  assumed,  therefore,  that  most  of  the  water  absorption  took 
place  during  blanching.   The  average  input/output  ratios  for  the  three 
varieties  tested  were:   spinach  -  1.54,  collards  -  1.34,  and  turnip  greens  - 
1.25,  indicating  the  relative  abilities  of  each  vegetable  to  absorb  moisture 
during  processing.   The  range  of  absorption  within  each  variety,  however,  was 
considerable  indicating  that  other  factors — such  as  initial  leaf  condition 
(turgid  or  wilted),  age,  size,  etc. — would  have  effects.   Particularly  note- 
worthy is  the  fact  that  variation  in  moisture  content  of  the  fresh  product 
(8.8%)  was  much  greater  than  that  of  the  packaged  product  (3.2%).   Plant 
records  indicate  that  overall  ratios  for  packaged  product  to  product  deliver- 
ed from  the  field  are  approximately  0.75  for  turnip  greens,  0.90  for  collards 
and  0.95  for  spinach.   These  ratios  include  wastage  such  as  the  losses  on  the 
dry  inspection  belts  and  the  gains  due  to  water  absorption.   They  vary  con- 
siderably both  within  seasons  and  from  year  to  year. 

40 


3  4 

OPERATING    TIME    (HRS  1 


Figure  16: 


Product  flow  rate  vs.  operating  time,  trial  2,  Fall,   19*5,  wlien  processing 
collards  with  the  prototvpe  systeo.  Fresli  product  into  first  washer. 


2 

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^'^11,    197S,   wlicn   procciiKlng   L-ollArils   with    the   prototvpe 
sv;tP"-       Fresh   product    into   llrnt   wjbIut. 


41 


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Grit  Accumulation 

Removing  accumulated  grit  from  the  washers  is  one  of  the  clean-up  tasks 
required  at  the  end  of  a  shift,  or  sooner  if  necessary.   Mechanical  or 
hydraulic  means  for  continuous  removal  could  have  been  incorporated  into  the 
experimental  system  at  considerable  expense.   An  expedient,  but  workable, 
alternative  used  in  the  operation  of  the  conventional  washers  was  to  open 
the  first  drain  valve  on  the  first  washer  for  a  few  seconds  as  necessary  to 
"flush  out"  excess  grit,  because  most  grit  tends  to  settle  out  immediately 
beneath  the  fresh  product  input.   This  technique  was  not  often  required, 
although  it  did  increase  the  effort  needed  in  clean-up  when  used. 

The  amount  of  grit  on  incoming  product  varies  greatly  depending  on  the 
particular  type  green  being  processed.   Spinach  is  usually  the  "dirtiest" 
because  the  convolutions  in  the  leaf  surfaces  tend  to  trap  soil  particles 
and  because  it  grows  close  to  the  ground.   Collards,  on  the  other  hand,  have 
smooth,  waxy  surfaces  and  grow  erect.   Turnips  greens  are  intermediate  in 
these  characteristics.   Other  factors  include  soil  splashing  from  recent 
rains  or  sprinkler  irrigation,  age  of  the  leaves  (older  leaves  tend  to  be 
larger,  smoother  and  cleaner) ,  and  the  cutting  (first  cuttings  are  made 
closer  to  the  ground  than  subsequent  ones) . 

Table  9  summarizes  the  measurements  of  accumulated  grit  in  the  trials  of 
the  prototype  washer.   The  collards  processed  during  the  fall  trials  1 
through  4  were  very  clean,  leaving  very  little  grit  in  the  system.   During 
these  trials,  several  techniques  were  tried  to  capture  or  collect  the  grit 
as  the  water  flowed  out  of  the  drains.   In  trial  5  (spinach)  a  measurable 
amount  of  grit  accumulated  in  the  system,  and  it  was  scooped  out  of  each 
tank  after  the  tank  had  been  drained  over  the  top  with  a  sump  pump.   This 
technique  was  used  in  the  spring  trials  with  the  prototype  and  required 
approximately  an  hour's  work  for  each  clean-up  plus  considerable  agility  on 
the  part  of  the  clean-up  crew. 

The  majority  of  the  grit  collection  took  place  in  the  first  washer  (38% 
avg.)  and  in  the  first  settling  tank  (43%  avg.).   Only  4  percent  was  collected 
in  the  second  washer  and  15  percent  in  the  second  settling  tank.   Although 
the  figures  for  the  maximum  amount  in  each  trial  vary  between  washer  1  and 
settling  tank  1,  the  amount  accumulated  in  washer  2  was  always  the  lowest 
for  all  four  units.   In  only  one  case  (trial  6,  spring)  did  settling  tank  2 
collect  more  grit  than  settling  tank  1.   These  figures  strongly  indicate 
that  the  majority  of  grit  removal  took  place  in  the  first  washer  sub-system. 

The  washers  each  had  three  drains,  located  at  the  apex  of  the  V-shaped 
bottom  sections  (Figure  3) .   Approximately  60  percent  of  the  grit  collected 
in  each  of  the  washers  settled  in  the  first  V-section,  26  percent  in  the 
second  and  13  percent  in  the  third.   Figures  18  and  19  show  the  summation 
percentages  of  the  particle  size  analyses  for  grit  from  the  various  units  of 
the  prototype  system  for  trial  5  in  the  fall.   Figures  A-31  through  A-34, 
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indicate:  (1)  that  most  of  the  larger  particles  (>100  y)  settle  out  in  the 
washers,  (2)  that  the  settling  tanks  were  removing  some  particulate  matter 
smaller  than  the  design  diameter  of  50  y,  and  (3)  there  was  little  or  no 

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47 


variation  in  the  particle  size  distribution  of  grit  from  spinach,  collards  or 
turnip  greens.   These  analyses  should  be  useful  for  designers  developing 
similar  equipment  for  product  grown  on  sandy  and  sandy-loam  soils.   For 
other  soils,  particle-size  analyses  would  have  to  be  developed. 

The  effectiveness  of  grit  removal  in  the  settling  tanks  could  probably 
be  improved  by  using  a  lower  recirculation  rate.   Necessary  hydraulic  agita- 
tion could  be  provided  by  increasing  the  pressure  on  the  spray  nozzles  in 
the  washers.   The  surges  in  flow  to  the  settling  tanks  produced  by  the  sump 
pumps  probably  reduced  effectiveness  of  the  tanks.   This  problem  could  be 
eliminated  by  locating  the  settling  tanks  beneath  the  washers  so  that  they 
would  receive  the  overflow  by  gravity. 

The  amount  of  time  required  to  remove  acciamulated  grit,  and  the  con- 
figuration of  the  conventional  washers  made  it  impossible  to  measure  the  grit 
collected  in  them  during  the  trials.   Only  in  trial  2  (spring)  was  the  amount 
of  grit  on  incoming  product  significantly  different  from  that  processed 
during  the  prototype  runs.   Accumulated  grit  had  to  be  shoveled  out  of  the 
first  conventional  washer  during  the  lunch  break — approximately  1700  kg. 
Visual  observation  of  the  conventional  washers  in  the  other  trials  indicated 
that  the  amount  collected  was  of  the  same  order  of  magnitude  as  that  collected 
in  the  prototype  washers.   Again,  most  of  this  accumulation  was  in  the  front 
section  of  the  first  washer.   Only  limited  amounts  accumulated  in  the  second 
washer.   Samples  for  particle  size  analysis  were  taken  during  trial  2  (Figure 
20) .   Because  the  accumulated  soil  had  reached  a  level  within  a  few  inches 
of  the  water  level  in  the  washer  where  smaller  particles  could  be  deposited 
it  is  assumed  that  these  results  are  representative  of  the  total  soil  brought 
in  on  the  product.   If  true  then,  86  percent  of  the  grit  to  be  removed  from 
the  product  was  in  the  size  range  above  100  microns. 

Trash  Accumulation 

Two  operational  characteristics  can  be  derived  from  the  trash  collection 
data  for  the  prototype  system.   They  are:   (1)  the  amount  of  waste  product 
that  would  have  to  be  disposed  of  and  (2)  the  amount  expected  to  be  lost 
during  washing.   Table  10  summarizes  these  data  and  indicates  that  neither 
of  the  above  would  be  a  major  concern.   The  data  for  equivalent  weight  of 
fresh  product  lost  to  trash  was  determined  by  adjusting  the  wet  weight  of 
the  trash  to  the  average  moisture  content  of  the  incoming  product  during 
each  trial. 

The  trash  generated  by  the  prototype  consisted  primarily  of  leaf 
particles.   This  type  of  material  from  the  conventional  washer  flowed 
directly  into  the  waste  stream  and  had  to  be  removed  by  vibrating  screens 
before  the  plant  effluent  was  released  to  treatment  facilities. 


PRODUCT  QUALITY  PARAMETERS 

The  product  quality  parameters  measured  were  insect  counts,  bacterial 
contamination  and  grit  on  the  leaves.   Of  these,  only  insect  and  bacterial 


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49 


counts  were  made  during  the  fall  trials.   The  hand  washing  test  for  grit 
analysis  was  not  devised  until  the  start  of  the  spring  trials. 

Insect  Counts 

Insect  infestation  of  product  was  extremely  low  during  the  fall  trials 
and  not  very  severe  during  the  spring  trials.   Though  fortunate  for  Exmore 
Foods,  this  s'.tuation  did  not  allow  for  a  very  rigorous  test  of  the  proto- 
type for  removing  insects.   Insect  counts  for  trial  5  (fall)  and  trials 
1-6  (spring)  are  included  in  Tables  B-1  through  B-7  in  Appendix  B.   A  total 
of  only  10  insects  were  found  on  all  the  product  samples  taken  during  trials 
1  through  4  in  the  fall  with  no  more  than  2  in  any  one  sample. 

Significant  insect  populations  on  the  product  appeared  only  during 
trial  2  (spring)  when  the  prototype  was  not  in  operation.   In  this  trial 
the  two  conventional  washers  removed  63%  of  the  insects  (aphids)  from  the 
spinach,  and  for  all  trials  averaged  62%.   Whether  or  not  this  could  be 
considered  representative  is  not  known. 

Several  bits  of  evidence  indicate  that  the  prototype  was  effective  in 
removing  insects.   First,  the  data  show  that  it  was  consistent  in  lowering 
insect  counts  as  the  product  was  washed.   Second,  there  was  no  evidence  of 
build-up  of  insects  or  insect  fragments  on  the  product  as  time  proceeded. 
This  is  significant  in  view  of  the  fact  that  the  wash  water  was  being 
recirculated.   Finally,  counts  on  the  trash  collected  from  the  washers 
during  the  fall  averaged  124  whole  insects  and  81  fragments  per  100  grams 
even  though  incoming  product  averaged  only  4  whole  and  1  fragment  per  100 
grams  of  sample  taken.   Over  all  trials  the  prototype  removed  70%  of  all 
insects. 

Grit  on  Product 

Figures  21  through  24  depict  some  of  the  results  of  the  hand  washing 
tests  of  product  samples  for  grit  analysis  in  the  spring  trials.   Complete 
data  are  included  in  Tables  B-8  through  B-13,  Appendix  B.   The  amount  of 
grit  on  entering  product  varied  greatly.   The  amount  on  product  leaving  the 
first  washer  varied  less  than  initial  readings  and  that  leaving  the  second 
washer  even  less.   In  all  four  trials  where  both  the  prototype  and  conventional 
systems  were  operated  the  prototype  removed  a  greater  percentage  of  the  grit  - 
averages  of  73  percent  and  69  percent  respectively. 

Spinach  harbored  more  grit  than  turnip  greens.   In  trial  1  the  prototype 
removed  80%  of  the  grit  and  the  conventional  washers  73%.   The  conventional 
washers  averaged  70%  removal  in  trials  1,  2  and  3  (spinach).   The  maximum 
amount  of  grit  observed  on  incoming  spinach  was  22,000  mg   per  lOOOg  product, 
10  times  greater  than  the  maximum  observation  for  turnip  greens,  2070  mg  per 
lOOOg  of  product.   In  trials  4,  5,  and  6  (turnip  greens)  the  prototype  aver- 
aged 64  percent  removal  and  the  conventional  washers  59  percent.   Removal 
percentages  for  turnip  greens  appeared  to  be  lower  in  general  than  those  for 
spinach.   This  probably  relates  more  to  the  amount  of  grit  on  product  than 
any  other  factor  in  these  trials.   Given  several  varieties  of  greens  of  "equal 
dirtiness"  spinach  would  undoubtedly  be  the  most  difficult  to  clean. 

50 


6  O 


10 


ACCUMULATED     PRODUCT     INPUT.    KG  X   10 


ACCUMULATED      PRODUCT    INPUT,    KG  X  10 


Figure   21:      Crlt    {inorganic    solids)    on   splnacli   vs.    accumulated 

product   at    three   sttes   in  prototvpe   svstcm.    Trial    I, 
Spring;    unwaslied   product    (Site    1) ,    product    ex  i  ting 
first   washer    (Site    3),    product   exiling   second  washer 
(Site  i>). 


figure    22  :      (^r  i  r     ( ln»i  ftani  r    snl  i,K)    .-ii   •^piiiAi  '>   vs.    a(-t'ur>ti>lat<  ■! 
product   al    tliroc   sites    in   cnveni  ional    syiic:-,    Tri^ 
I ,    Spring;    unwashed   product    (Sin-    7)  ,    product    pxit  i 
firsE  wASher    (Site   8),    product   exiting   s^ecoiiJ  wa^-io 
(Site   10). 


leo 


O   13  5 


O     9  0 


7  8 

ACCUMULATED    PRODUCT    INPUT,    KG  X  10^ 


)  12  3  4 

ACCUMULATED    PRODUCT     INPUT,    KG  X 10 


Figure   23:      Crit    (inorganic    solids)    on    turnip   greens    vs. 

accumulated   product    at    three   bltos    in   prototype 
svstem.    Trial    6,    Spring;    unwashed    product    (Site    1), 
product   exiting    first   washer    (Site    i) ,    product 
exiting  second  washer    (Site  4). 


'  rlt    (iiiurganic    solid^^)    on    turnip   greens   vs. 
accumulated    produn    .it    three   sites    in   ccnveniicna] 
svstein,   Trul    h.    Spring;    unwashed   product    (9,itc    12), 
product    cxiiiiiji    (irst   w.isl.er    (Site    14).    product 
exiting  secoiid  washer    (Sltr    15). 


51 


The  greater  percentage  removal  of  grit  by  the  prototype  over  the  conven- 
tional system  may  be  attributed  to  the  increased  hydraulic  agitation  and 
the  final  fresh  water  rinse.   However  there  was  a  decrease  in  the  percent 
of  grit  removal  with  time  in  three  of  four  trials,  probably  due  to  the  use 
of  recirculated  water.   Grit  removal  in  the  conventional  washers  varied 
randomly  with  time. 

Bacteria  Counts 

Bacterial  counts  for  both  product  and  water  samples  are  not  as  complete 
as  originally  planned  for  a  variety  of  reasons  —  including  considerable 
variation  in  the  amount  of  bacteria  on  input  product,  problems  in  transporting 
samples  under  controlled  conditions  and,  in  general,  the  rather  primitive 
laboratory  facilities  on  site.   The  data,  however,  are  sufficient  for  certain 
inferences. 

Bacteria  counts  on  product  samples  are  included  in  Tables  B-14  through 
B-23,  Appendix  B.   Table  11  is  a  summary  of  the  principal  effects  on  product 
and  water  bacteria.   Over  all  trials,  the  prototype  and  the  conventional 
systems  each  reduced  the  bacterial  counts  on  product  74  percent  of  the  time. 
Of  special  significance,  however,  are  the  prototype  trials  in  the  spring  — 
1,  4,  5,  6.   Unfortunately,  a  spreader- type  organism  present  in  the  water 
overgrew  the  plates  in  trial  4,  obscuring  the  colonies.   In  the  other  three 
trials,  however,  there  was  a  very  consistent  and  obvious  lowering  of  bacterial 
counts  (100%  of  the  time)  as  the  product  passed  through  the  system.   Undoubt- 
edly the  addition  of  fresh  chlorinated  water  as  a  final  rinse  on  the  product 
in  these  trials  had  an  influence  here. 

Factors  other  than  chlorine  in  the  wash  water  appear  to  be  operating 
in  the  reduction  of  bacteria  on  product.   It  may  be  that  something  from  the 
product  itself,  which  accumulates  in  the  water,  has  a  bacteriacidal  action. 
Again,  the  results  of  the  spring  trials  with  the  prototype  showed  a  consistent 
reduction  in  counts  in  the  first  washer  which  received  no  fresh  water  (Figures 
25  and  26) .   Counts  for  the  conventional  washers  did  not  exhibit  this 
consistency  (Figures  27  and  28).   Two  other  observations  can  be  made 
from  the  data  on  bacteria  counts.   1)  There  does  not  seem  to  be  a  clear 
relationship,  if  any  at  all,  between  counts  in  the  wash  water (data  presented 
below)  and  on  the  product.   2)  Though  not  obvious,  it  appears  that  higher 
initial  bacteria  counts  may  be  expected  on  spinach  than  on  collards  or  turnip 
greens.   There  are  many  uncontrolled  influences  here,  however. 

WATER  QUALITY  PARAMETERS 

A  total  of  10  different  water  quality  parameters  were  measured  during  the 
trials.   In  the  fall  these  included  bacteria  counts  (including  total  colif orms) , 
chlorine  residual,  BOD  ,  BOD   ,  COD,  TSS,  VSS,  turbidity,  color  and  pH.   In 
the  spring  bacteria  counts,  chlorine  residual,  COD,  TSS,  VSS  and  pesticide 
analyses  were  made. 


52 


2  50 


150 


50  ■ 


I 


Ni^i  [^  Ka 


I 


8 


01 

SITE   ,  I       3       4 

TIME      .25H0UR 


li 


i 


ill 


I 


4H0UR 


7  HOUR 


4   HOUR 


65  HOUR 


Figure  25:   Total  bacterial  place  counts  per  gram  of  spinach  at 

three  saiapling  points,  Trial  1,  Spring;  prototype  system. 
Ik-fore  washing  (Site  1).  exitinR  the  first  washer 
(Site  3),  exiting  the  second  washer  (Site  it). 


Figure  26:   Total  hacterial  plato  count'i  per  Rr.im  <>(  turnip  Krrfns 
at  three  sampling  points.  Trial  h,  spi ihr;  protntvpc 
system.   Before  washing  (Site  1).  exiting  the  (irsr 
washer  (Site  3).  exiting  the  second  wa-^her  (Site  4). 


a:     150- 


(00 


**00 


.1. 


! 


II 


4  HOUR 


TIME    25  HOUR      1  HOUR       4  HOUR 


Figure  27:   Toial  bacterial  plate  counts  per  gram  of  spinach 

at  three  sampling  points.  Trial  1,  Spring;  convention 
svscem.   Before  uasliing  (Site  7).  exiting  the  fir^^t 
washer  (Site  9),  exiting  the  second  washer  (Site  10). 


Figure  28:   r..tal  hacterial  plate  counts  per  gram  of  turnip  greens 
of  three  sampling  points.  Trial  6,  Spring;  conventional 
svstcm.   Before  washing  (Sltr  J2),  exiting  the  first 
w.ishcr  (Site  14),  exiting  the  second  washer  (Site  15). 


53 


TABLE  11.   COMPARISONS  OF  BACTERIAL  POPULATION  DENSITIES  (TOTAL  PLATE  COUNTS) 
FOR  PRODUCT  LEAVING  TO  PRODUCT  ENTERING  A  TWO-WASHER  SYSTEM  AND 
FOR  WATER  LEAVING  THE  SECOND  WASHER  TO  WATER  ENTERING  THE  FIRST 
DURING  GREENS  -  WASHING  TRIALS. 


Trial 

Product 

Water 

Product 

Total  Chli 

Drine, 

*** 

mg/L 

A* 

B** 

A* 

B** 

Beginning 

End 

Average 

Fall  trials,  prototyp 

e  washer 

1 

Collards 

3/3 

0.15 

- 

- 

2.16 

0.65 

1.10 

2 

Collards 

3/3 

0.02 

1/4 

2.17 

2.58 

0.0 

1.39 

3 

Collards 

3/3 

0.23 

3/4 

0.46 

0.4 

2.03 

1.35 

A 

Collards 

2/2 

0.09 

6/8 

0.64 

1.16 

2.30 

2.06 

5 

Spinach 

2/3 

0.98 

3/5 

0.50 

1.30 

1.50 

1.52 

Spring 

trials,  p 

irototype  washe 

r^ 

1 

Spinach 

3/3 

0.11 

3/3 

0.72 

1.30 

0.57 

0.61 

5 

Turnip 
Greens 

2/3 

0.73 

3/3 

0.33 

1.79 

0.68 

1.02 

6 

Turnip 
Greens 

2/2 

0.28 

3/3 

0.07 

1.32 

0.62 

0.91 

Spring 

trials,  conven 

tional  washers 

1 

Spinach 

2/3 

2.57 

2/2 

0.59 

1.23 

1.15 

1.07 

2 

Spinach 

- 

- 

2/3 

0.04 

0.28 

0 

0.47 

3 

Spinach 

1/3 

1.49 

3/3 

0.03 

0.23 

0 

0.08 

A 

Turnip 
Greens 

1/2 

1.15 

4/5 

6.26 

0.55 

0 

0.18 

5 

I'tirnlp 
Creens 

1/3 

L.22 

3/5 

0.  J8 

1.56 

0 

0.52 

6 

Turnip 
Greens 

1/3 

0.92 

3/5 

0.85 

0.54 

0 

0.18 

* 
A   =  ratio  of  number  of  observations  during  a  trial  that  the  bacterial  count 

was  lowered  (input  of  first  washer  to  output  of  second  washer)  to  total 
number  of  observations. 
** 

B  =  ratio  of  average  bacteria  counts  during  trial,  output  of  second  washer 

to  input  of  first. 
*** 

"  Residuals  measured  amperometrically  and  represent  concentrations  at  the 

back  of  the  second  washer  at  the  beginning  and  end  of  each  trial,  nnd 
the  average  for  the  entire  trial.  (3  to  8  observations  per  trial). 


54 


Bacteria  Counts  and  Chlorine  Residual 

Total  plate  count  data  are  recorded  in  tables  C-1  through  C-10,  Appendix 
C.   These  counts  ranged  over  5  orders  of  magnitude  during  the  fall  trials  and 
4  orders  of  magnitude  in  the  spring.   The  relative  counts  for  water  where  the 
product  entered  compared  to  those  where  it  was  discharged  in  the  prototype 
system  exhibited  some  consistency  as  shown  in  the  first  two  columns  of  Table 
11.   The  bacteria  count  was  lower  for  the  output  of  the  second  washer  compared 
to  the  input  of  the  first  washer  in  20  of  22  observations.   The  opposite  was 
true  in  the  conventional  washers  where  the  output  counts  were  lower  only  for 
6  of  14  observations.   These  last  results  seem  to  indicate  that  the  last  water 
the  product  is  exposed  to  does  not  necessarily  have  a  direct  effect  on  the 
product  bacteria  counts  which  were  consistently  lowered  by  the  conventional 
washers . 

The  response  to  chlorine  was  not  always  consistent  either  as  indicated 
in  Figures  29  and  30.   Total  chlorine  was  measured  and  recorded  each  time  a 
water  sample  was  taken.   Chlorine  input  to  the  fresh  water  in  the  plant  was 
manually  regulated  and  varied  considerably.   A  high  value  of  3.8  mg/£  was  re- 
corded in  the  fall  trials  and  a  high  of  1.79  mg/Jl  in  the  spring.   Concentra- 
tions usually  but  not  always  decreased  toward  the  end  of  the  trials  (Table  11) , 
often  falling  to  zero.   This  correlates  with  the  fact  that  73  percent  of  the 
counts  on  water  samples  taken  at  the  end  of  the  trials  (all  sites)  were 
greater  than  those  taken  at  the  beginning.   Fewer  (54  percent)  of  the  product 
samples  at  those  times  showed  higher  counts ,  and  the  magnitude  of  these 
changes  was  not  as  great  as  for  the  water  samples. 

The  data  in  Table  12  re-enforce  the  lack  of  correlation  between  bacteria 
in  water  and  on  product.   There  appeared  to  be  a  consistent  increase  in 
bacteria  counts  in  the  water  in  both  systems  as  time  proceeded.   The  build-up 
in  the  conventional  system  may  indicate  a  trend  to  higher  levels  because 
these  trials  were  usually  shorter  than  those  with  the  prototype.   The 
differences  in  counts  on  the  product  for  the  fall  and  spring  prototype  trials 
may  be  attributed  to  the  generally  higher  levels  of  chlorine  in  the  water 
during  the  fall.   Warmer  weather  in  the  spring  may  have  also  been  a  factor. 
The  differences  between  the  build-up  of  counts  on  product  for  the  prototype 
and  conventional  washers  in  the  spring  may  merely  be  the  influence  of  exposing 
the  product  to  more  chlorine  treated  water  in  the  conventional  washers. 
In  summary  the  influences  on  bacteria  counts,  both  product  and  water,  are  not 
clear.   A  system  similar  to  the  prototype  using  a  final  product  rinse  with 
closely  controlled  chlorine  content  would  appear,  however,  to  have  an 
advantage  over  conventional  washers  in  bacteria  control. 


55 


1/3W  '3Nia01H3    TViOi 


(  nw  a3d  jOix  S3iNonoo) 

INnOO    3iVld    "IViOi.    30   DOT 


1/  ow '  a^iHOHHO  nviOi 


—    3 


(  nw   y3d   jOIX  S31N0103) 

iNnoD  3ivnd  nwiOi  30  ooi 


56 


TABLE  12.   MAGNITUDE  OF  AVERAGE  CHANGES  IN  TOTAL  PLATE  COUNTS  FROM  BEGINNING 
TO  END  OF  TRIALS  AT  ALL  SAMPLING  SITES  RECORDED. 

Trials Water* Product** 

Prototype  (Fall)  +169.0  -110.3 

Prototype  (Spring)  +78.3  +  41.7 

Conventional +356.0 -  4.6 

_ 

Colonies  X  10  per  milliliter 
Colonies  X  10  per  gram 


Tables  C-11  through  C-15,  Appendix  C  record  data  from  the  fall  trials 
on  total  conforms  in  the  wash  water.   These  organisms  indicate  the  presence 
of  fecal  material  on  the  incoming  product.   Their  effect  on  final  product, 
however,  is  not  known. 

TSS,  VSS,  COD  and  BOD 

Figures  31  and  32  are  two  examples  of  water  quality  parameters  at 
strategic  points  and  times  during  the  fall  and  spring  trials  of  the  prototype. 
In  both  cases  the  waste  strength  parameter  is  plotted  vs.  accumulated  fresh 
product  input  to  the  washers  during  the  trial.   All  parameters  in  all  trials 
tended  to  follow  similar  patterns.   Data  on  all  trials  are  contained  in 
Tables  C-16  through  C-57,  Appendix  C. 

The  six  sites  in  the  figures  represent  sampling  locations  (refer  to 
Figures  1  or  12)  and  differences  between  adjacent  sites  in  the  flow  pattern 
represent  the  effect  of  a  certain  component  of  the  system  on  the  quality  of 
the  wash  water.   Values  at  site  2  minus  those  at  site  1,  for  instance, 
represent  the  amount  of  a  waste  component  added  to  the  wash  water  in  washer  1; 
similarly  for  sites  3  and  4  in  washer  2.   Sites  2  and  6  bracket  the  effects 
of  the  sump  pump  and  moving-screen  belts  in  sub-system  1;  sites  4  and  5  in 
sub-system  2.   Sites  6  and  1  bracket  the  effects  of  the  settling  tank  in  the 
first  sub-system;  sites  5  and  3  in  the  second.   Site  1  also  represents  the 
overflow  to  waste  for  the  entire  system. 

The  most  obvious  fact  from  these  graphs  is  the  considerable  difference 
in  waste  strength  in  the  first  sub-system  (sites  1,  2,  and  6)  compared  to  the 
second  (sites  3,  4,  5).   Approximately  75  percent  of  the  product  cleaning 
took  place  in  the  first  washer  and  settling  tank  based  on  analysis  of  grit 
accumulated  in  the  bottom  of  the  tanks  and  remaining  in  the  water  at  the  end 
of  each  trial.   These  graphs  also  indicate  the  general  effectiveness  of  the 
moving-belt  screens  and  settling  tanks.   The  effectiveness  of  the  settling 
tanks,  as  noted  earlier,  could  be  improved  by  lowering  the  recirculation 
rate  and  eliminating  the  surges  in  flow  caused  by  the  intermittant  operation 
of  the  sump  pumps.   Also,  the  settling  tanks  apparently  performed  better  in 

57 


250 


225 


.200 


cn 

Q 

_i 
o 

CO 

Q 
UJ 
Q 

■z. 

Ld 
Q. 
(/5 

3 
If) 

_l 
O 


175 


150 


125 


100 


0123456789 

ACCUMULATED  PRODUCT  INPUT, kgxIO^ 


Figure  31:   I'Hal  5;uspended  solids  vs.  accumulated  product  input 

at  .ill  six  sampling  !;ltes.  Trial  '<,  Fall,  uhen  process! 
cul lards  with  prototype  system. 


200 


100 


Figure    32 


ACCUMULATED       PRODUCT      INPUT,         KG  X   10 


il.rimcal    oxygon    drmand    vs.    acriimu  lai  cd    |>roilucl    at    nil    six 
-i-impilnR   «;Iti.s.    Trial    U ,    ■Spring,    when   prcccssing   lurnJp 
greens   with   prototvpc    sv^lem. 


J  200 


'0  1  2  3  'i 

ACCUMULATED    PRODUCT     INPUT,    KG    X 


o  SI  rE  I-' 

o  SITE  n 

•  SITE  I^ 

■  SITE  1^ 


ACCUMULATED     PRODUCT     INPUT,   KG    X     10^ 


Figure  33.  Total  suspended  ';olld<;  vi .  accumulated  prod 
at  all  four  sampling  siti--;.  Trial  1,  Spring 
spinach   processed   with    conventional    washer. 


Figure     ^u.     Cliemical   oxygen   demand   vs.    arcumulated   product   at 
all    four    sites.    Trial    b.    Spring,    turnip   greens 
processed  with  conventional   system. 


58 


the  fall  trials  than  the  data  indicates  due  to  the  method  of  taking  samples 
at  sites  5  and  6  (input  ends  of  the  tanks).   In  the  fall  trials,  water  samples 
were  taken  at  these  points  by  dipping  the  container  into  the  surface  of  the 
water.   Some  rapidly  settling  solids  may  have  been  missed  by  this  technique. 
In  the  spring  trials,  these  samples  were  taken  by  holding  the  container  under 
the  moving-screen  belts  to  catch  the  samples  before  the  water  entered  the 
tanks . 

Ideally,  a  waste  strength  parameter  in  any  one  of  the  washers  or  settling 
tanks  in  a  recirculating  system  should  increase  by  some  relationship  such  as: 

X  =  A(l  -  e'^"^) 

where : 

X  =  concentration  of  the  particular  parameter  at  any  time,  t. 
q  =  total  material  processed  to  time,  t. 
A  and  B  =  constants. 

In  other  words,  given  constant  inputs  -  i.e.,  constant  rate  of  material 
input  of  constant  "dirtiness"  and  a  constant  fresh  water  input  rate  —  the 
waste  strength  parameter  should  approach  the  asymptotic  value  A  with  time. 
Material  input  rates,  the  amount  of  soil  on  the  vegetables,  and  even  the 
range  in  volatiles  produced  varied  too  much  to  be  able  to  make  precise 
predictions  of  concentrations.   In  general,  however,  it  appears  that  the 
water  quality  parameters  in  the  prototype  system,  operated  as  in  these  trials, 
could  be  expected  to  stablize  in  approximately  5  hours.   Leaving  the  fresh 
water  on  during  breaks  in  processing  would,  of  course,  tend  to  dilute  the 
waste  strength  parameters,  (note  dip  in  values  of  TSS  for  first  sub-system 
in  Figure  31) .   Very  low  fresh  water  input  rates  as  in  trial  4  in  the  spring 
(Figure  32)  would  tend  to  increase  the  time  until  stability  is  reached. 
Accumulations  of  dissolved  organics  may  also  have  the  effect  of  lowering  the 
emission  rates  of  COD  and  BOD.   The  decrease  in  osmotic  gradients  between 
the  product  and  the  wash  water  could  reduce  the  leaching  of  these  materials 
as  washing  proceeds  with  recirculated  water. 

Twenty-day  BOD  values  were  taken  on  some  of  the  water  samples  during  the 
fall  trials.   These  are  tabulated  in  Tables  C-54  through  C-57,  Appendix  C. 

Figures  33  and  34  show  examples  of  water  quality  parameters  vs. 
accumulated  product  for  the  sampling  sites  on  the  conventional  washers.   The 
waste  strength  parameters  are  again  plotted  vs.  accumulated  fresh  product  in- 
put to  the  washers.   Generally  the  concentrations  in  the  first  washer  (sites 
7  and  8  or  12  and  13)  were  higher  than  those  in  the  second  (sites  9  and  10 
or  14  and  15).   This  was  not  always  consistent,  strongly  affected  by  the 
amount  of  water  used  in  each  washer.   Waste  strength  also  varied  considerably 
from  beginning  to  end  of  the  trials  and  inconsistently  with  the  amount  of 
product  processed.   This  inconsistency  was  the  result  of,  not  only  variations 
in  incoming  product  quality,  but  also  in  water  flow  rates. 


59 


pH  was  measured  and  recorded  every  time  water  samples  were  taken. 

The  range  in  the  fall  trials  was  6.5  to  7.6  and  in  the  spring  trials  7.6 

to  8.5.   These  ranges  do  not  indicate  any  problems  for  waste  treatment  due 
to  pH. 

Color,  Turbidity  and  Conductivity 

Readings  on  color,  turbidity  and  conductivity  were  taken  and  recorded 
during  the  fall  trials  with  the  prototype.  These  parameters  generally 
followed  the  trends  of  the  other  waste  strength  parameters.   These  measurer 
ments  are  easy  to  make  and  any  or  all  of  them  may  provide  simple  means  for 
controlling  the  operation  of  recirculating  systems  in  the  future.   For 
example,  Figures  35,  36  and  37  present  simple  regressions  for  BOD  vs. 
color  for  trials  2,  3,  and  4  (fall)  with  collards.   Further  study  on 
these  general  relationships  for  each  commodity  appears  warranted. 


Pesticides 

Water  samples  for  pesticide  analysis  were  taken  midway  and  at  the  end  of 
each  trial  of  the  prototype  at  the  overflow  of  washer  1  (site  2)  during  the 
spring  trials.   These  samples  were  analyzed  for  Phosdrin,  the  insecticide 
used  by  Exmore  Foods.   The  results  are  listed  in  Table  13  below. 


TABLE  13.   CONCENTRATION  OF  PHOSDRIN  IN  WATER  OF  FIRST  WASHER  OF  PROTOTYPE 
SYSTEM,  SPRING  TRIALS 


Trial 


Water/Product 
ii-/kR 


Hours  of  Operation 


Concentration 
ppb* 


2.18 


3 
5 


1.45 
0.81 


3.99 


0.17 


4.22 


7 

4 


non-detectable 

Trace 
(<  0.01) 


6.5 


non-detectable 


Parts  per  billion. 


Samples  from  Trial  1,  the  first  of  the  four  trials  with  the  prototype 
in  the  spring  became  overheated  in  transit,  began  to  decompose  anerobically, 
and  consequently  could  not  be  analyzed.   Samples  from  the  other  trials  indicate 
that  1)  concentrations  of  pesticide  were  very  low,  2)  they  tended  to  decrease 
with  time  rather  than  build  up  in  the  recirculated  water  and  3)  they  tended 
to  decrease  with  increased  water/product  ratios. 

60 


MO 

• 

/ 

130 

/ 

120 

•                             / 

/      Y<O49X-0l0 
R=0  9l 

110 

/ 

100 

■                                  •       / 

. 

90 

(7 

80 

70 

60 

•  / 

50 

/ 

40 

30 

"  "/. " 

20 

-  •  / 

10 

'■III 

1            ■ 

100 

- 

90 

- 

.  / 

80 

- 

'/ 

70 

- 

/    Y=  I.34X-6 

60 

- 

.          / 

/               R=095 

50 

- 

/ 

40 

- 

/• 

30 

, 

•     / 

20 

,                / 

<;•• 

10 

50        100       ISO      200     250      300 
COLOR  IN  KLETT    UNITS 


20       30       40       50       60       70 
COLOR   IN   KLETT    UNITS 


Figure  J5.  FjvL-Jav  biochemical  oxvgcn  dcmnn.l  «<; .  color.  Trial  2, 
Fall,  ulien  processing  collards  with  prototype  svsten. 


Figure  ^h.   Flvc-riay  hiochenical  oxvgcn  demand  v 
when  processing  collards  with  protol 


.■lor.  Trial  3.  Fall. 


120 

- 

/ 

110 

- 

/ 

KDO 

- 

•        • 

/. 

90 

- 

•.■ 

'        Y=  I43X+  836 

80 

- 

, 

/. 

R=  0  77 

70 

- 

•  • 

/ 

60 

- 

• 

/ 

50 

- 

••/ 

40 

■ 

/     . 

. 

30 

_ 

/ 

" 

20 

/ 

10 

r    : 

10       20       30       40       50       60       70       80       90 
COLOR   IN   KLETT    UNITS 

Figure   37.  Five-day  bloi hemlcal  oxvgcn  demand  vs.  color.  Trial  U.    Fall, 
when  processing  collards  with  prototype  system. 


61 


SUMMARY  OF  WASTE  PRODUCTION  FROM  WASHERS 

Waste  is  carried  from  the  washers  by  the  water  in  three  different 
ways:   1)  with  the  effluent  during  processing ,  2)  with  the  water  carried  out 
of  the  system  on  the  product  and  3)  with  the  water  dumped  from  the  washers 
(and  settling  tanks  for  the  prototype)  at  the  end  of  a  processing  shift. 
Tables  14,  15  and  16  show  the  amounts  of  each  waste  parameter  measured  in 
these  trials  per  metric  ton  of  product  processed.   Concentrations  for  each 
time  period  multiplied  by  average  water  flow  during  the  period  were  summed 
for  an  entire  trial  to  obtain  the  total  waste  leaving  in  the  effluent. 
Similarly,  the  concentrations  in  the  last  washer  multiplied  by  average 
product  flow  and  amount  of  water  leaving  per  unit  of  product  were  also 
summed.   The  amounts  of  waste  in  the  washers  and  settling  tanks  at  the  end 
of  processing  was  determined  by  multiplying  the  final  concentrations  by  the 
volume  of  the  tanks  in  which  they  were  measured. 

Tables  15  and  16  (spring  trials)  show  the  amounts  of  waste  leaving  the 
washers  by  each  route.   The  percentages  in  each  case  varied  considerably. 
The  waste  leaving  the  prototype  (Table  15)  on  the  product  may  be  slightly 
less  than  estimated  here  because  of  the  final  rinse  on  the  discharge  belt  of 
the  second  washer.   One  consistency  to  be  noted  for  the  three  trials  when 
turnip  greens  were  processed  (4,  5,  6)  is  that  the  percentage  of  waste 
removed  by  the  system  overflow  increased  very  rapidly  as  the  amount  of  fresh 
water  input  increased. 

There  are  at  least  three  things  that  affect  total  waste  production  and 
waste  stream  concentrations.   They  are:   1)  the  variety  of  vegetable  being 
processed,   2)  the  amount  of  water  per  unit  of  product  processed,  and   3) 
the  condition  of  the  vegetables.   Potter  (15)  shows  that  collards  are  high  in 
nutritive  value  —  7.5  vs.  4.3  grams  of  carbohydrates  and  40  vs.  26  calories 
per  100  grams  compared  to  spinach.   This  implies  that  there  should  be  con- 
siderable difference  in  VSS  and  COD  production  from  different  varieties. 
On  the  other  hand.  Bough  (2)  found  that  spinach  produced  significantly 
higher  waste  loads  during  washing  when  compared  to  collards,  turnip  greens, 
mustard  and  kale.   The  results  of  this  study  however,  do  not  indicate  that 
any  one  of  the  three  products  tested  could  be  used  as  a  model  for  maximum 
VSS  and  COD  emission.   Emission  rates  for  TSS  were  consistently  higher  for 
spinach.   The  combination  of  savoy  leaf  surfaces  and  low  growth  profile 
undoubtedly  increases  grit  accumulation  compared  to  other  leafy  vegetables. 

The  amount  of  water  used  to  wash  a  unit  of  product  appears  to  have  con- 
siderable influence  on  waste  production.   VSS  and  COD  mass  emission  rates 
were  consistently  lower  for  the  prototype  than  for  the  conventional  system. 
There  are  at  least  four  possible  reasons  for  this:   1)  The  concentrations 
of  these  waste  components  in  the  recirculated  water  of  the  prototype  were 
considerably  higher  than  those  in  the  conventional  washer.   The  more  dilute 
and  larger  amounts  of  water  used  in  the  conventional  washers  may  have 
affected  the  surface  of  the  leaves  and  induced  more  leaching  of  organics, 
a  possibility  noted  by  EPA  (7) .  2)  The  water  used  in  the  conventional  washers 
was,  for  the  most  part,  taken  from  the  product  cooling  flumes  and  did  contain 
some  soluble  material.  3)  A  significant  amount  of  these  components  may  have 
left  the  system  with  the  leaf  fragments  separated  out  by  the  moving  belt 

62 


TABLE  14.   WASTE  LOADS  DISCHARGED  WITH  WATER*  FROM  PROTOTYPE  SYSTEM 
DURING  FALL  TRIALS,  1975 


Trial 


Product 


Waste  Stream/ 
Product 
(Ji/kg) 


Waste  Load,  kg/metric  ton+ 
TSS    VSS    COD    BOD^ 


1 
2 
4 
5 


Collards 
Collards 
Collards 
Spinach 


1.97 
0.69 
1.30 
1.55 


0.38 

0.19 

0.92 

0.25 

0.27 

0.21 

0.77 

0.16 

0.43 

0.24 

0.88 

0.22 

2.44 

0.21 

0.91 

0.17 

(15.0)** 

Sum  of  wastes  carried  out  overflow  of  system  during  trials,  plus  waste 
in  water  carried  out  on  product,  plus  waste  in  water  remaining  in  system 
at  end  of  processing. 


** 


Grit  collected  from  bottom  of  washers  and  settling  tanks  at  end  of  trial. 
Amount  of  grit  from  this  source  was  negligible  in  other  trials. 


Waste  loads  given  in  kg/metric  ton  of  fresh  (raw)  product  entering  washing 
system.  See  Table  6  for  factors  to  convert  these  readings  to  field  weight 
or  packaged  weight. 


63 


TABLE  15.   WASTE  LOADS  DISCHARGED  WITH  WATER  FROM  PROTOTYPE  SYSTEM  DURING 
SPRING  TRIALS,  1976 


Trial 


Product 


Waste  stream/ 

Product     Waste* 
Jl/kg       Source 


Waste  Load,  kg/metric  ton"*"  and 
percent  of  total 


TSS 


VSS 


COD 


Spinach 


1.38 


Turnip 
Greens 


Turnip 
Greens 


Turnip 
Greens 


0.16 


1.35 


1.68 


A 
B 
C 
TOTAL 
D 

A 
B 
C 
TOTAL 
D 

A 
B 
C 
TOTAL 
D 

A 
B 
C 
TOTAL 
D 


0.65(25.4%) 

0.97(38.2%) 

0.93(36.4%) 

2.54(100%) 

5.30 

0.43(38.1%) 
0.06(  4.9%) 
0.64(57.0%) 
1.13(100%) 
2.10 

0.19(32.5%) 

0.20(34.2%) 

0.20(33.3%) 

0.59(100%) 

1.10 

0.06(  6.9%) 

0.53(65.6%) 

0.22(27.5%) 

0.81(100%) 

2.90 


0.11(26.6%) 
0.15(38.0%) 
0.14(35.4%) 
0.40(100%) 


0.07(44.8%) 
O.OK  6.9%) 
0.07(48.3%) 
0.15(100%) 


0.04(38.1%) 
0.03(28.6%) 
0.04(33.3%) 
0.11(100%) 


O.OK  8.3%) 
0.08(66.7%) 
0.03(25.0%) 
0.12(100%) 


0.37(32.4%) 
0.39(34.7%) 
0.37(32.9%) 
1.13(100%) 


0.20(35.2%) 
0.02(  3.6%) 
0.34(61.2%) 
0.56(100%) 


0.13(27.7%) 
0.15(31.9%) 
0.19(40.4%) 
0.47(100%) 


0.04(  8.2%) 
0.32(64.3%) 
0.13(27.5%) 
0.49(100%) 


A  -  Carried  out  with  water  on  product. 

B  -  Discharged  from  settling  tank  #1. 

C  -  Remaining  in  water  in  system  at  end  of  processing. 

TOTAL  -  Total  of  wastes  from  water  in  system 

D  -  Total  grit  collected  in  the  bottom  of  the  washers  and  settling  tanks 
at  the  end  of  each  trial. 

+  -  Waste  loads  given  in  kg/metric  ton  of  fresh  (raw)  product  entering 
washing  system.  See  Table  7  for  factors  to  convert  these  readings 
to  field  weight  or  packaged  weight. 


64 


TABLE  16.   WASTE  LOADS  DISCHARGED  WITH  WATER  FROM  CONVENTIONAL  WASHERS  DURING 


SPRING  TRIALS,  1976 


Trial 


Product 


Spinach 


Spinach 


Spinach 


Turnip 
Greens 


Turnip 
Greens 


Turnip 
Greens 


Waste  stream/ 
Product     Waste* 
Jl/kg       Source 


Waste  Load,  kg/metric  ton"*"  and  per- 
cent of  total 


17.5 


4.1 


9.8 


17.6 


16.0 


20.7 


TSS 


A  1.74(12.2%) 

B  12.10(85.1%) 

C  0.39(  2.7%) 

TOTAL  14.23(100%) 

A  4.30(46.5%) 

B  4.52(48.9%) 

C  0.43(  4.6%) 

TOTAL  9.25(100%) 

A  0.81(16.8%) 

B  3.55(74.2%) 

C  0.43(  9.0%) 

TOTAL  4.79(100%) 

A  0.24(  9.3%) 

B  2.26(87.4%) 

C  0.09(  3.3%) 

TOTAL  2.59(100%) 

A  0.12(  8.1%) 

B  1.24(87.3%) 

C  0.07(  4.6%) 

TOTAL  1.43(100%) 

A  0.21(14.6%) 

B  1.14(79.2%) 

C  0.09(  6.2%) 

TOTAL  1.44(100%) 


VSS 


0.04(10.4%) 
0.29(85.1%) 
0.02(  4.5%) 
0.35(100%) 

0.03(13.0%) 
0.19(82.6%) 
0.01(  4.4%) 
0.23(100%) 


COD 


0.22(13.0%)  0.39(  8.7%) 

1.37(82.5%)  3.93(87.1%) 

0.07(  4.5%)  0.19(  4.2%) 

1.66(100%)  4.51(100%) 

0.40(42.0%)  0.65(46.8%) 

0.51(54.3%)  0.65(46.8%) 

0.04(  3.7%)  0.09(  6.4%) 

0.95(100%)  1.37(100%) 

0.15(17.2%)  0.56(15.9%) 

0.62(73.4%)  2.59(74.1%) 

0.08(  9.5%)  0.35(10.0%) 

0.85(100%)  3.50(100%) 

0.04(10.3%)  0.45(13.5%) 

0.29(85.3%)  2.74(81.9%) 

0.02(  4.4%)  0.15(  4.6%) 

0.35(100%)  3.34(100%) 


0.49(15.1%) 
2.57(79.3%) 
0.18(  5.6%) 
3.24(100%) 

0.42(18.9%) 
1.62(73.6%) 
0.16(  7.5%) 
2.20(100%) 


A  -  Carried  out  with  water  on  product. 

B  -  Discharged  from  washers  1  and  2 

C  -  Remaining  in  water  in  system  at  end  of  processing. 

TOTAL  -  Total  of  wastes  from  water  in  system.  Grit  remaining  in  bottom  of 
washers  at  end  of  each  trial  could  not  be  collected  without  inter- 
f erring  with  plant  operations. 

+  -  Waste  loads  given  in  kg/metric  ton  of  fresh  (raw)  product  entering  wash- 
ing system.   See  Table  8  for  factors  to  convert  these  readings  to  field 
weight  or  packaged  weight. 


65 


screens.   4)  Some  of  these  components  may  have  settled  out  with  the  finer 
soil  particles  in  the  prototype  settling  tanks.   Soil  samples  taken  from 
these  tanks  were  observed  to  be  high  in  organic  matter. 

The  amount  of  grit  removed  by  the  water  from  the  conventional  system 
(TSS  minus  VSS)  appears  to  be  greater  than  that  for  the  prototype  system. 
No  direct  comparisons,  however,  can  be  made  because  the  grit  collected  in  the 
bottom  of  the  conventional  washers  could  not  be  measured  in  these  trials. 
The  prototype  showed  a  consistent  advantage  over  the  conventional  washers  in 
reducing  the  amount  of  grit  on  product  samples  that  were  hand  washed  as 
described  earlier.   Assuming  that  this  is  a  correct  representation  of  the 
relative  effectiveness  of  the  two  systems,  then  the  prototype  has  the  added 
advantage  of  consolidating  more  of  the  wastes  in  its  washers  and  particularly 
in  the  settling  tanks  for  disposal  separate  from  washer  effluents. 

Emission  rates  of  the  various  waste  components  are  also  strongly 
influenced  by  the  conditions  of  incoming  product  within  a  variety.   This 
condition  is  influenced  by  many  things  including  maturity  of  plants,  growing 
conditions,  whether  wilted  or  turgid,  rain  or  irrigation  prior  to  harvest,  etc. 
These  effects  are  demonstrated  by  the  data  for  each  variety  presented  in 
Tables  14,  15,  16  and  in  Table  17  (described  below).   Even  if  these  data  were 
"normalized"  to  constant  amounts  of  water  per  unit  of  product  processed  there 
would  still  be  considerable  differences  in  TSS,  VSS  and  COD  per  kg  of  product 
within  each  variety. 

Table  17  is  a  summary  of  average  waste  stream  size  and  average  waste 
component  concentrations  for  each  of  the  trials,  fall  and  spring.  These  data 
do  not  reflect  the  changing  concentration  of  waste  components  in  the  prototype 
system  with  time.   They  should,  however,  be  useful  in  planning  for  design 
of  a  waste  treatment  system  that  uses  either  conventional  or  recirculating 
washers. 

Overall  average  operating  conditions  for  the  prototype  washing  system 
during  these  trials  included  a  product  input  rate  of  1278  kg/hr  and  fresh 
water  input  of  72  X,/min.   Under  these  conditions  2.20  Jl/kg  left  the  system 
with  the  product,  1.18  left  via  the  waste  stream  and  the  waste  concentrations 
(TSS,  VSS,  COD)  in  the  various  units  of  the  system  could  be  expected  to 
stabilize  in  approximately  five  hours  of  continuous  operation.   The  number 
of  trials  run  were  insufficient  to  indicate  whether  or  not  these  were 
"optimum"  conditions.   Nevertheless,  they  appear  to  be  "good"  or  minimum 
conditions  for  producing  suitably  clean  product.   For  all  product  flow 
rates  a  minimum  fresh  water  input  of  3.5  Jl/kg  (0.42  gal  /lb)  is  recommended. 


ECONOMIC  COMPARISONS 

The  following  is  an  example  problem  to  demonstrate  the  comparative 
economics  of  owning  and  operating  a  two-washer  prototype  system  vs.  a  two- 
washer  conventional  system  of  equal  output.   Many  variables  will,  of  course, 
affect  this  type  of  comparison.   The  basis  here  is  an  assumed  "reasonable" 
set  of  operating  and  economic  conditions. 


66 


TABLE  17.   WASTE  STREAM  CHARACTERISTICS  FROM  PROTOTYPE  AND  CONVENTIONAL 
SYSTEMS 


Product 

Average  Waste 
Stream  ± 
«,/min 

Avg. 

Waste  Cone. 

.*,  mg/Ji 

Trial 

TSS 

VSS 

COD 

BOD 

1-F*-P+ 

Collards 

38.2 

80.8 

30.6 

137.6 

46.8 

2-F-P 

Collards 

20.0 

91.7 

68.6 

264.1 

62.6 

3-F-P 

Collards 

18.9 

158.0 

95.0 

346.3 

62.5 

4-F-P 

Collards 

25.8 

121.4 

65.9 

127.7 

57.0 

5-F-P 

Spinach 

25.5 

516.8 

45.6 

144.0 

42.3 

1-S-P 

Spinach 

29.5 

818.5 

126.9 

331.1 



4-S-P 

Turnip 
Greens 

3.9 

414.5 

48.0 

191.7 



5-S-P 

Turnip 
Greens 

24.1 

194.4 

28.0 

131.7 

— — — 

6-S-P 

Turnip 
Greens 

37.7 

230.0 

35.6 

139.9 



1-S-C 

Spinach 

328.3 

1157.1 

103.1 

216.2 



2-S-C 

Spinach 

107.2 

3618.0 

168.9 

255.1 



3-S-C 

Spinach 

266.3 

336.7 

60.1 

228.9 



4-S-C 

Turnip 
Greens 

405.9 

129.3 

17.2 

156.2 



5-S-C 

Turnip 
Greens 

396.3 

70.6 

16.4 

147.8 



6-S-C 

Turnip 
Greens 

393.0 

54.9 

9.1 

78.6 

F  =  fall  trial,  S  =  spring  trial 

P  =  prototype,   C  =  conventional 

±  =  Values  are  averages  for  the  single  waste  stream  from  the  prototype 
system  and  are  weighted  averages  for  the  total  waste  flow  from  the 
two  conventional  washers. 


67 


Annual  Fixed  Costs 

The  assumptions  here  are:   1)  The  two  conventional  washers  will  cost 
$12,000  ($6,000  each.   Estimate  from  A.  K.  Robins  Co.,  Baltimore,  Md.)  and 
the  prototype  will  cost  $16,000.   The  prototype  cost  is  based  on  an  estimate 
of  1.33  times  that  of  the  conventional  washers  and  assumes  that  it  will  be 
constructed  to  include  the  simplifications  cited  in  the  recommendations 
(section  3  of  this  report).   2)  Useful  life  of  the  equipment  is  12  years  and 
salvage  value  at  the  end  of  usefulness  will  be  10%  of  initial  cost.   Straight 
line  depreciation  will  be  used.   3)  Interest  on  investment  will  be  equal  to 
8%  of  the  average  value  of  the  equipment  over  its  useful  life,  per  year;  and 

4)  cummulative  over  ownership  costs  (taxes,  housing,  insurance)  are  equal  to 
2%  of  the  initial  cost  per  year.   The  following  annual  costs  are  derived 
using  these  assumptions. 


Item 

Depreciation 

Interest  on  investment 

Taxes,  housing,  insurance 

Totals 


Washer 

Line 

Conventional 

Prototype 

$  900 

$1200 

516 

688 

240 

320 

$1656 


$2208 


Yearly  difference:    $2208  -  1656  =  $552.00 


Operating  Costs 

Operating  costs  and  variables  are  taken  from  local  information 
(Blacksburg,  Va.)  and  conditions  comparable  to  those  reported  in  this  study. 
Only  the  waste  stream  from  the  washers  is  considered  in  computing  sewer 
charges.   Assumptions  include: 


Item 

Hours  of  operation 

Man-hours  labor/day 

Labor  cost,  $/hr 

Product  throughput,  Ib/hr 

Input  water  rate,  gal/min 

Water  for  filling  system,  gal. 

Waste  stream,  gal/min 

Waste  production,  into  waste 

stream,  lbs/ton  of  product, 

(Assumes  that  3/4  of  the  product 

processed  is  spinach,  1/4  turnip 

greens) : 

TSS 

VSS 

COD 


Washer 

Line 

Conventional 

P] 

rototype 

16 

16 

18 

20 

3.5 

3.5 

3000 

3000 

100 

20 

1500 

3000 

85 

6.5 

11.53 
1.49 
5.14 


3.16 
0.48 
1.33 


68 


Power  to  operate  washers,  kw 
Electricity  costs,  c/kwh 
Fresh  water  cost,  $/1000  gal 
Sewer  charge  for  water,  $/1000  gal 
High  strength  surcharge  rates,  <?/lb 
TSS  above  200  mg/«, 
COD  above  120  mg/ I 
Repairs  and  maintenance, 
(0.02%  of  initial  cost/hr.)  $/hr. 


3.0 
3.4 
0.5 
1.0 

10 
7 

2.40 


14.6 
3.4 
0.5 
1.0 


10 
7 

3.20 


Using  the  above  assumptions  the  following  daily  operating  costs  were  calcu- 
lated: 


Item 


Washer  Line 
Conventional        Prototype 


Electric  Power 

$  1.62 

$  7.96 

Water  (including  two  flll-ups/ 

day) 

49.50 

12.60 

Sewer  Charges 

84.60 

12.24 

Sewer  Surcharges 

14.03 

4.38 

Repairs 

28.40 

51.20 

Labor 

63.00 

70.00 

TOTAL 

$251.15 

$158.38 

Daily  difference  $251.15-158.38  =$92.77 

Under  the  assumed  conditions  for  this  problem,  then,  the  average 
annual  difference  in  owning  the  two  types  of  systems  could  be  recovered  in 
slightly  less  than  6  days  of  operating  time. 


69 


REFERENCES 


1.  Agricultural  Research  Service,  1963.  "Composition  of  Foods."  Agriculture 
Handbook  No.  8,  USDA,  Washington,  D.  C. 

2.  Bough,  W.  A.  1973.  "Composition  and  Waste  Load  of  Unit  Effluents  from 

a  Commercial  Leafy  Greens  Canning  Operation."  Journal  of  Milk  and  Food 
Technology,  Vol.  36,  No.  11,  pp.  544-553. 

3.  Buckman,  H.  0.,  and  N.  C.  Brady.  1969.  The  Nature  and  Properties  of 
Soils,  7th  Edition,  The  MacMillan  Co.,  Printed  in  U.S.A. 

4.  Carter,  L.  W.  1970.  "A  Study  of  Water  Conservation  and  Reuse  at  the 
Stillwell  Canning  Co.  in  Stillwell,  Oklahoma."  Report  prepared  for 
the  Ozarks  Regional  Commission. 

5.  Day,  P.  R.  1965.  "Hydrometer  Method  of  Particle-Size  Analysis." 
Methods  of  Soil  Analysis,  Part  1,  Amer.  Soc.  Agron.,  Madison,  Wis. 

6.  Directory  of  the  Canning,  Freezing  and  Preserving  Industries.   1971. 
E.  E.  Judge,  and  Sons  Publishers.  Westminister,   Maryland. 

7.  E.P.A.  1976.  "Final  Effluent  Guidelines  and  Standards  for  Phase  II 

of  the  Canned  and  Preserved  Fruits  and  Vegetables  Processing  Industry 
Point  Source  Category."  Federal  Register.   Chapter  I.  Subchapter  N, 
Part  407. 

8.  Frey,  B.  C.  1973.  "Modification  of  a  Leafy  Vegetable  Immersion  Washer." 
Unpublished  Master's  Thesis,  Virginia  Polytechnic  Institute  and  State 
University. 

9.  Frey,  B.  C,  M.  E.  Wright,  and  R.  C.  Hoehn.  1974.  "Modification  of  a 
Leafy  Vegetable  Immersion  Washer."  Transactions  of  the  A.S.A.E., 
Vol.  17,  No.  6,  pp.  1057,  1058,  1059  and  1063,  St.  Joseph,  Mich. 

10.  Holtan,  H.  N.,  N.  E.  Minshall,  and  L.  L.  Harrold.   1962.   Field  Manual 
for  Research  in  Agricultural  Hydrology.   Agricultural  Research  Service. 
Agricultural  Handbook  No.  224,  Washington,  D.  C. 

11.  Lopez,  A.  1969.   A  Complete  Course  in  Canning,  9th  Edition.  The  Canning 
Trade,  Baltimore,  Maryland. 

12.  Mercer,  W.  H.   1956.  "Canner  Foods."   Industrial  Waste  Water  Control. 
Edited  by  C.  F.  Guinham,  Academic  Press,  New  York,  n.  Y.,  pp.  65-71. 


70 


13.  Metcalf  &  Eddy,  Inc.   1972.  Wastewater  Engineering.  McGraw-Hill  Book 
Co.,  New  York,  N.  Y. 

14.  National  Canners  Association.  1971.   "Liquid  Wastes  from  Canning  and 
Freezing  Fruits  and  Vegetables."  Water  Pollution  Control  Research 
Series,  12060  EDK-08/71.   U.S.  Government  Printing  Office,  Washington, 
D.  C. 

15.  Potter,  N.  N.   1968.   "Food  and  Waste."  Food  Science,  The  AVI  Publishing 
Co.,  Inc.,  Westport,  Conn.,  pp.  471-587. 

16.  Ramseier,  R.  E.   1942.  "The  Evaluation  of  Industrial  Wastes  in  the  East 
Bay."  California  Sewage  Works  Journal,  XIV,  No.  1,  pp.  26-37. 

17.  Robinson,  W.  H. ,  Jr.,  and  M.  E.  Wright.  "A  Note  on  Plexiglass  H  S 
Flumes."  Water  Resources  Research.   In  press. 

18.  S.C.S.  Engineers.   1971.   Industrial  Waste  Study  on  Canned  and  Frozen 
Vegetables,  Interim  Report,  Contract  No.  68-01-0021  for  the  U.S. 
E.P.A. ,  Long  Beach,  California. 

19.  Standard  Methods  for  the  Examination  of  Water  and  Wastewater.   1971. 
Edited  by  AWWA,  APHA,  and  WPCF,  13th  Edition. 

20.  Townsend,  C.  T.,  I.  I.  Somers,  F.  C.  Lamb,  and  N.  A.  Olsen.   1956. 
A  Laboratory  Manual  for  the  Canning  Industry,  2nd  Edition,  National 
Canners  Association  Research  Laboratories,  Washington,  D.  C. 

21.  U.S.  Department  of  Agriculture.  1971.   Agricultural  Statistics. 
Government  Printing  Office,  Washington,  D.  C. 


71 


APPENDIX  A 


460 
440 
420 


•METER  I 


METER  2 


METER  5 


o -METER  2 


METERS 


OPERATING  TIME   (HRS.) 


Plpire  A-1:   Water  flow  racee  vs.  operating  tine,  trial  2,  Pall.  1975. 
whan  prooeaslog  collardi  with  prototype  waahcr. 


OPERATINO  TIME  (HRS) 

Figure  A-2:   Hater  flow  races  ve.  operating  tlac.  trial  3,  Pall,  1975, 
when  processing  collards  with  prototype  waaher. 


400 

1 

380 
360 

en 

1 

340 

b. 

80 

a: 

60 
40 

20 

^i— METER  3 


_i_ 


2  3         4  5         6  7 

OPERATING    TIME    (HRS) 


Figure  A-3:      Uater   flow  race*   vs.    operating   clBc,    irlal   it,   Fail,   1975, 
when  proceaalog  collards  with  prototype  ayaceiii. 


560 

540 

520 

500 

480 

^ 

^ 

460 

^ 

*< 

e 

440 

3 

420 

i 

400 

fH 

K 

i 

380 

■METER  2 


METER  I 


METER  5 


0  12         3         4        5 

OPERATWG   TIME   (HRS.) 

Figure  *-«;  Hater  flow  rates  vs.  operating  clac,  trial  5.  Pall. 
1875.  when  proceaaing  aplnach  wlch  prototype  ayetea. 


72 


^^^ 


OPERATING  TIME  (hrs.) 

Figure  A-5:   Water  flow  rates  vs.  operatlnR  time.  Trial  i.  Spring, 
1*176,  when  processing  turnifi  (treens  with  prototypp 
systes. 


Figure  A-6:   Untct  flr>w  rates  ■ 


OHtRATINC  TIME  (hrs.) 

oper.irloR  time.  Tri.il  5.  Sprinc, 


when  processing  turnip  greens  with  prototype  washer. 


HETF.R  3 
METER  <• 
METER  5 


3       4      S      6 
OrERATINC  TIME  (hrs.) 


TOTAL 

WASIIKR  1 
WASHi.k  2 


Figure  A-7i   Water  flow  rale*  vs.  oporatlng  tlin'.  Trial  6,  SprlnR.  1976. 
when  processing  turnip  grecn.«  with  prototype  washer. 


OPERATINC  TlHV  (hrs.) 


Water  overflow  ralefi  from  conventional  washers  vs. 
operating  time.  Trial  2.    Spring.  1975,  when  processing 
spinach  on  the  cast  line. 


73 


500 

- 

1       1       I      1       1 

1 r 

400 

- 

■ 

300 

^ 

-r 

»-. 

■ 

200 

• 

, — i 

i-i 

■ 

100 

— 1 

1 1 —  ..  i      1       1 

Ed  200 


-I \ r 


OPERATING  TIHF  (hrs.) 

Figure  A-9:   Water  overflow  rates  from  conventional  washers  vs. 

operating  time.  Trial  3,  Spring.  1976,  wlien  processing 
spinach  on  the  west  line. 


(TEKATINC  TI^U  (hrs.) 


Klgiirc  A-tO:   Wnter  over  fltiw  rotes  from  c<invrnt  Ioii.tI  w^iHhcr>i  vs. 

oiiomtinR  linu-.  Trl.il  '4,  Spring,  iy7f».  when  proc.-;slnR 
turnip  greens  on  west  line. 


,  WASllEH  1 
^WASHER  1 


500 

- 

■  T- 

T    ■  "t 

_  100 

E 

* 

— 

^^    TOTAL 

■ 

- 

a  100 

• 

^    WASHTH  1 

2 

s 

K  200 

, 

^ 

5 

100 

^  WASHER  2 

1       1       1 

1              1 

OPERATING  TIME  (hrs.) 

Figure  A-II.   Water  overflow  rates  from  convent lon^il  washers  vs.  operating 
time.  Trial  5.  Spring.  1976,  when  processing  turnip  greens 
on  west  line. 


ni'EKATIHr.  TUU   (hr.s) 

Figure  A-U:  Uator  overflow  rates-  from  tonvont  f  on.il  washers  vs. 
operating  time.  Trial  fi.  Spring,  l')76,  when  procctJ 
turnip  greens  on  west  line. 


74 


■ 

(D 

sx^ 

' 

3 

"x^ 

■             I 

<    ^\ 

111             x, 

i          1          i          1       \ 

i 

i 

UJ 


o 
o 
o 


o 
o 
o 


o 
o 
o 


o 
o 
o 


OX)  indNi  ionaoad  oaivinwroDv 


S- 


JM/fin  '3iva  Monj  ionooMd 


(aH/ox)  31VH  AMTij  ionootw 


75 


■9  « 


(^H/>:i)  2vn  noii  i^aaond 


(tiH/OX)  31VU  MOId  lOnOOHd 


<  ^  "> 


(9)1)  indNi  ionooHd  03i.vnnwnD3v 


-J  a 
-«  o 


3    —    . 


OX)  indNi  ionooHd  aaxvnnwnoov 


76 


S   9000 

s 

a   75O0 

u 

g   6000 


^ 

^ 

y^ 

/           ^ 

. 

/  */^^                  • 

PROTOTYPE 

, 

y/\^^                                                * 

CONTONTIONU. 

(East    Line) 

r            X                t               •                1               • 

OPERATING  TIME  (hrs) 


Figure  A-20:   Accunulited  product  input  vs.  operating  tine,  Trl«l  1, 
Spring,  1976,  when  processing  spinach. 


3000 

2  700 

\        . 

21i0O 

/           . 

5    2100 

/ 

A          / 

ju  leoo 

/\                    /\        / 

g     1500 

e! 

/         N,^,--*.          /      \         /          ■ 

y  1200 

\y  \  /  ■ 

a. 

900 

\  /   ■ 

60O 

V 

300 

1 1 1 L..                 .... 

"ll'KRATINr.  rJMK  (lir<) 


Figure  A-21;   Product  flow  ratr  vs. 


1 1 1 r 

12000 

/■ 

« 10500 

/     - 

£     9000 

y^-^       . 

H 

y 

=     7500 
1 

y 

o     bOOO 

s 

.J 

/" 

• 

1     4500 

, 

< 

300O 

y' 

- 

1500 

■    y^ 

G 

Z3 

o  u 


6000 

• 

4500 

y 

3000 

^y 

1500 

^      1        1        >        •        1        •        I        1 

operating  tiw.  Trial  2.  Spring. 


0        12        3      4        5      6       7       8 
OPERATING  TIME  (hrs.) 

Figure  A-22:   Accumulated  product  Input  vs.  operating  tine.  Trial  2, 

Spring,  1976,  when  processing  spinach  with  the  conventional 
washers .  east  line. 


1976  when  procesRlng  spinach  with  the  conventional  washi-rs. 
east  line. 


3       ft        5 

OPERATING  TIME  (hrs) 

Figure  A-21;   iToJuct  flow  rate  vs.  onerntlng  tftne.  Trial  3,  Spring, 
1976,  when  processing  spinach  with  the  conventional 
washers,  west  line. 


OPERATlNt;  TIME  (hrs) 

Figure  A-2A:  Accumulated  product  Input  vs.  opi-rotlng  time.  Trial  3, 
Spring  1976,  when  processing  spinach  with  conventional 
washers,  west  line. 


77 


2100 

\ 

j: 

IHOI) 

^^~^^ 

. 

3 

___/^ 

\                A 

2 

1300 

^ 

^^\ 

. 

• 

i 

1200 

• 

V      . 

\ 

• 

a. 

900 

, 

\^^ 

, 

600 

^ 

• —    rRtTOTYPE 

300 

* —  CiiNVENTIONAL 

(Went    line) 

1                 1                  .                 I 

• 

0         12        3       4        5       6        7       8 
OPERATING  TIME  (hrs.) 

Figure  A-25:   Product  flow  rate  vs.  operating  time.  Trial  4,  Spring 
1976,  when  processing  turnip  greens. 


KOOU 
/■)(HI 

^ 

B 

ftOUO 

/^ 

c 

o 

4  500 

- 

y^ 

\ 

3000 

• PHOTOTYPE 

1500 

— * CONVfNTIONAL 

(W.St    llnel 

OPJRATIKC  TIMK  (hrs) 


Figure  A-26i   Accumulated  product  input  vs.  operating  tine.  Trial  k. 
Spring.  1976,  when  processing  turnip  greens. 


moo 

. 

T        "T       I 1 r- 

^ 

1500 

/ 

p>^ 

■ 

< 

1200 

-        /              / 

\.      y 

■ 

1 

900 

w^^ 

6 

o 

s 

a. 

600 

•         / 

—■ • mOTOTVPE 

■ 

r 

1       . 

(Wfst    line) 
1                  1                 I                  i                  , 

3       4       5      6 
OPERATING  TIME  (hrs) 


Figure  A-27:   Product  flow  rate  vs.  operating  time.  Trial  5.  Spring, 
1976.  when  processing  turnip  greens. 


J    7500 

■    T 1 1 1 r- 

•^ 

A.     60U0 

. 

y^ 

S    4500 

y    /^ 

■ 

ACCUMULATED 

X          y^                              * 

IMiMirrvfK 

/    y^              --*- 

i:nKVF.NTio;i.M. 

(kost    line) 

3       u  5 

Orr-RATINC  TIME  (lirs) 


rigurc  A-28;   Accumulated  product  Input  vs.  oper.iting  time.  Trial  5, 
Spring,  1976,  when  processing  turnip  greens. 


-• —     I'RinOTYPE 

A  COHVKNTlONAI. 

(WcHC    line) 


90U0 

• 

1 1 T-  -       T      r 

7500 

- 

y 

■ 

6000 

■ 

y^ 

■ 

4500 

/O 

■ 

300» 

^                                    — • PKOTUTYPE 

■ 

^""^               1                                   I 

* —   Cl'NVENTlOKAl 

OPKRATINC  TIME    (hrs) 


A-30:      Ai'cuiTiulatcd    priKJuct    Input    vs.    oprrjttng    tlmo.    Trial    6, 
RprlnR,    1976,    vlien   proces<;lng    turnip   greens. 


OPERATING  Tl^ff    Oirs) 

Figure  A-29:      Product    flow   rate    va.    operating    time.    Trial    6,    Spring, 
1976,   wh«n  proceaalng   turnip  greens. 


78 


S3JVJ.N33H-I.1    NOIlVWWnS 


S'JDVJ.H33HHd    NOIlVHHnS 


N  o   e 


snoviNHnan.!  Noiiviwns 


SHtJVlN^DUtH.r      NOllVHHnS 


79 


APPENDIX  B 


TABU  B-1. 

TOrrU.    IKSECIS  ON    PRODUCT   SAMPLES      OP  SPIHACH  GREENS. 
TRIAL   S,    PALL,    DECEHBCR   IS,    1975 

Hours  of  Operation 

Sice 

1.0                     2.0                     3.0                     t.O 

12 
0 
10 


100  graa  saaples,  replicated  values. 


9 

10 


TABLE  B-2.   TOTAL  INSECTS  ON- SAMPLES*  OF  SPINAOI  CREEHS, 
TRIAL  1,  SPRING,  APRIL  22.  19?t. 


Houra 

0 

f  Operation 

Sice 

.2S 

1 

2 

3 

«              5 

6 

7 

1 

0 

- 

- 

0 

2              1 

0 

0 

3 

0 

- 

- 

0 

0              1 

0 

0 

k 

0 

- 

- 

1 

0              1 

0 

0 

100  graa  saaplea 


TABLE  B-3.   TOTAL  INSECTS  AND  FRAOIENT  COUNTS  ON  PRODUCT  SAMPLES  OF  SPINACH  GKEEHS,  TRIAL  2.  SPRING, 
MAY  12.  1976 


Houra  ot  Operation 


Ina.  Frag?   Ins.  FraR.   Ins.  Frag.   Ina.  Frag.   Ins.  Frag.   Ins.  Frag.    Ins.  Frag. 


71"   K"    91    23 


'lOO  gran  sanples    Average  of  Two  VaLues     Fragments     Inaeccs 


TABLE  B-4.   TOTAL  INSECTS  AND  FRAGMENT  COUNTS  ON  PRODUCT  SAMPLES*  OF  SPINACH  GREENS.  TRIAL  3.  SPRING. 
HAY  21.  1976 


Hours  of  Operation 


Ins.  Frag. 


Ins.  Frag. 


Ins.  Frag. 


100  graa  saaplas 


80 


lA  lO 

d 


sg 


I  I 


I  r  I 


o  o 


o  o 


o  o  o  o 


o  o  o 


I 

m 

i 

u 
ee 

8 


I 
9 

M 

S 


05, 


G2 
S   ■ 

O  -^ 
K  -4 

w 

s 

8 


000 


000 


o  o 


81 


TABLE   B-9.      MILLIGRAMS   OF  GRIT   TtR  KILOGRAM  OF   PRODUCT   KOR  TRIAL   2. 


TABLL   B-IO.      MILLIGRAMS   OF  GRIT   PER   KUAXIRAM  OF   PRODUCT   FOR  TRIAL   3, 
SfRINC.    WASHING  OF   SPINACH   GREENS,    MAY   21.    1976 


SPRING,  yASHI 

INC  OF  SPINACH  CKEENS,  HAT  IZ 



— 



' 

Hnurs  of  Operation 

SlEC 

.25 

Hours 

n_f 

Dpctilt 

Ion 

3              5 

7 

2 

7 
9 
10 

6255 

1845 
1275 

22275          4245 
6525          1920 
3765          1215 

3510 
1920 
1080 

12 
U 
IS 

1740 
1230 
660 

1890 
1230 
900 

TABLE   8-11.      HILLICRAMS  OF  CRIT   PFR  KILOGRAM  OF  PRODUCT    FOR  TRIAL   4, 
SPRING.    WASUmC  OF  TURNIP  GREENS.    JimL   4.    1976 


TABLE   8-12.      MILLIGRAMS   OF  CRIT   PER   KILOCRAM  OF   PRODUCT   FOR   TRIAL   5, 
SPRING,    WASHING   OF  TURMIP  GREENS,    JUNE    10.    1976 


Hours  of  Operation 


1 
3 
4 
12 
14 
15 


.25 


2070 
1080 
673 
835 
613 
375 


1560 
855 
765 

11B5 
750 
630 


1530 
1035 
660 
750 
285 
270 


690 
675 
480 


Hours  of  Operation 


Site 

.25 

2 

4 

6.5 

1 

1005 

945 

1230 

615 

3 

300 

352 

1410 

451 

4 

195 

300 

345 

242 

12 

615 

1)95 

1005 

- 

14 

300 

720 

570 

- 

15 

345 

510 

465 

- 

TABLE  B-13.   MILLIGRAMS  OF  CRIT  PER  KILOGRAM  OF  PRODUCT  FOR  TRIAL  6, 
SPRING,  WASHING  OF  TURNIP  GREENS.  JUNE  11,  1976 


TOTAL  PLATE  COUNT   (COLONIES  XIO   PER  GRAM)  ON  PRODUCT  FROM 
PROTOTYPE  FOR  TRIAL  2,  FALL,  WASHING  OF  COLLARD  GREENS, 
NOVEMBER  4,  1975 


1 
3 
i 
12 
U 
13 


.25 


1080 
343 
ISO 

1125 
675 
435 


Hours  of  Operation 


Hours  of  Operation 


1740 
840 
330 
750 
630 
433 


915 
660 
375 
1935 
945 
510 


840 
840 
480 


1.7 

4.1 
6.0 


7.3 

3.5 

1.7 

11.6 

54.5 

2.7 

5.3 

11.2 

8.3 

Each  value  is  average  of  two  reading: 


TABLE  B-15.   TOTAL  PLATE  COUNT   (COLONIES  XIO  PER  CRAM)  ON  PRODUCT  FROM 
PROTOTYPE  FOR  TRIAL  3.  FALL.  WASHING  OF  COLLARD  GREENS, 
NOVEMBER  20,  1975 


682.0 
509.0 
2S0.O 


Hours  of  Operation 


1227.0 
473.0 
163.0 


350.0 

109.0 

1418.0' 


7 


359.0 

682.0 

80.0 


TABLE  B-16.   TOTAL  PLATE  COUNT  (COLONIES   XIO"'  PER  GR,SM1  ON  PRODUCT  FROM 
PROTOTYPE  FOR  TRIAL  4,  FALL,  WASHING  OF  COLLARD  GREENS, 

DECEMBER  I,  1975 


Hours  of  Operation 


0.25 


160. 0 
180.0 
80.0 


''        140.0     290.0     420.0     150.0     430.0     630.0     180.0 
20.0         -  100. 0     350.0       30.0         4.0     120.0 


520.0       40.0     100. 0     650.0       50.0         1.0 


70.0 


'single  value,  other  values  are  average  of  two  readings. 


Cram  positive  rods. 

Single  vaiuL.,  other  values  are  aver.igc  of  two  readings. 


82 


TABLE  B-U.   TOTU,  PLATE  COUNT  (COLONIES  Xio'  PER  CRAM)  ON  PRODUCT  FROM 

PROTOTYPE  FOR  TRIAL  5,  FALL.  WASHING  OF  SPINACH  GREENS. 
DECEMBER  15.  1975  


TABLE  B-I8.   TOTAL  PLATE  COUNT  (COLONIES  XIO  PER  CRAM)  FOR  PRODUCT  FROM 

PROTOTYPE  AND  CONVENTIONAL  SYSTEMS  FOR  TRIAL  1.  SPRING. 
WASHING  OF  SPINACH  CRLENS.  APRIL  22.  19 ?6 


Site 

3 

1 

! 

1 

Sit 

1 

600 

o' 

2090 

0' 

210 

0' 

820 

0= 

170 

0-= 

3 

710 

o' 

100 

0^ 

230 

o' 

- 

140 

0* 

i 

255 

o' 

150 

0' 

1090 

0' 

110 

0"^ 

300 

0' 

10 

Average 

of 

two  values. 

Average 

of 

three 

values 

Average 

of 

four 

or 

more  values 

Hours  of  Operation 


.25 


164.0 
14.7 
6.0 

155.0 
27.3 
96.4 


245.0 
222.0 
52.7 
31.6 
218.0 
14.2 


7 


265.0 
182.0 
104.0 


TABLE  B-19.   TOTAL  PLATE  COUNT  (COLONIES   XIO  PER  GRAM)  FOR  PRODUCT  FROM    TABLE  B-20.   T(7TAL  PLATE  COUNT  (COLONIES   XIO   PER  GRAM)  KOR  PRODUCT  FROM 

CONVENTIONAL  SYSTEM  FOR  TRIAL  2.  SPRING,  WASHING  OF  SPINACH                 CONVENTIONAL  SYSTEM  FOR  TRIAL  3.  SPRING.  WASHING  OF  SPINACH 
GREENS.  HAY  12.  1976  GREENS.  MAY  21.  1976 


Hours  of  Operation 


Hours  of  Operation 


9 

10 


35.5 
2.1 
1.6 


95.5 
1.2 
0.9 


0.6 
0.4 
2.9 


12 
14 
IS 


3.3" 

0.2^ 
0.3 


4.1" 

2.6 

0.7 


136.0' 
1.8" 
2.0' 


Average  o(  two  values. 


TABLE  B-21.   TOTAL  PLATE  COUNT  (COLONIES   XIO   PER  CRAM)  FOR  PRODUCT  FROM 

CONVENTIONAL  SYSTEM  FOR  TRIAL  4.  SPRING,  WASHING  OF  TURNIP 
GREENS,  JUNE  4,  1976 


TABLE  B-22.   TOTAL  PLATE  COUNT  (COLONIES   XIO  PER  CR.\H)  FOR  PRODUCT  FROM 

PROTOIYI'L  AND  CONVI.NTIONAL  SYSTLHS  FOR  TRIAL  5,  SPRING. 
WASHING  OF  TURNIP  GREENS.  JUNE  10,  1976 


12 
It 
IS 


.25 


4.1 

0.7 

1S7.0 


Hours  of  Operation 


Hours  of  Operation 


4.6 
4.3 
1.7 


5.6 
S.6 
3.0 


2.1 
2.« 


5.5 


Site 


3.5      1 

2.3      3 

1.5     4 

12 


14 


0.2 

- 

- 

- 

0.1 

- 

- 

- 

0.1 

- 

- 

- 

2.6 

3.7 

61.8 

10.1 

1.2 

0.5 

0.8 

11.9 

2.8 

0.5 

19.5 

4.8 

0.4 
0.3 
0.2 
2.9 
3.1 
3.8 


I.l 
0.1 
0.2 


TABLE  B-23.   TOTAL  PLATE  COUNt'  (COLONIES   XIO  PER  GRAM)  FOR  PRODUCT  FROM 
PROTOTYPE  AND  CONVENTIONAL  SYSTEMS  FOR  TRIAL  6.  SPRING, 
WASHING  OF  TURNIP  GREENS.  JUNE  11,  1976 


Hours 

of 

Operation 

Site 

.25 

1 

2 

3 

4 

6 

1 

2.32 

- 

- 

- 

0.9 

10.7 

3 

0.7 

- 

- 

- 

0.6 

O.i 

i 

0.3 

- 

- 

- 

0.4 

0.3 

12 

20.5 

2.3 

15.7 

8.1 

1.4 

- 

14 

0.4 

9.1 

2.8 

1.7 

1.9 

- 

IS 

1. 5 

29.3 

2.4 

6.2 

1.6 

- 

Average  of  two  values. 


83 


APPENDIX  C 


TABLE  C-l.      TOTAL   rL*Tt  COUNT    (COLONIES''   X    10     PER  HH.Llt.lTEII)    IN   WASH 
WATER   FROM  PROTOTYPE   FOR  TRIAL   1.    FALL,   WASHING  OF  COLLARO 
CREEBS,   OCTOIIER  24.    1975 ^_^ 


Hourit  of  QpTntlon 
1 


ua.o 

0.2 
0.2 
U.6 
13.3 
10.2 


10.2 
IS.O 
33.0 
6.0 
O.S 

s.s 


232.0 
>30.0'' 
3.3 
3.9 
110.0 
710.0 


Slca 
1 


TOTAL  PUTE   COUNT    (COLONlt.s   X    10"^   PKR  MILI.I  LITER)    IN   WASH 
WATER   FROM   PROTOTYPE   FOR  TRIAL   i.    FALL,    WASHING  OF  COLLARD 
GREENS.   NOVEMJER  4,    H;-; 


.0.25  . 
15. t*^ 
24.7"= 
U.O' 
14.0"= 
13.7' 

le.o' 


Houra  of  Operation 

)_ 

345.0'' 

561.0^ 

2.7" 

7.6' 

3.2^ 

1573.0" 


4 

1177.5' 

1065.0' 

9.2'> 

3.8" 

12.7" 

1270.0*' 


Average  of  two  values.  ""Average  of  ihrce 


'aluea.    Average  of  four  values 


Average  of  nore  Chan  one  value.   Insufficient  dilutions  before  placing. 


TABLE  C-4. 


TOTAL  PLATE  COUNT  (COLONIES  X  lo'  PER  MILLILITER)  IN  WASH 
WATER  FROM  PROTOTVPt  FOR  TRIAL  6.  FALL  WASHING  OF  COLLARD 
GREENS.  DECEMBER  1.  n;s ^^ 


TABLE  C-3.   TOTAL  PLATE  COUNT  (COLONIES  X  10   PER  MILLILITER)  IN  WASH 

WATER  FROM  PROTOTYPE  FOR  TRIAL  3,  FALL,  WASHING  OF  COLLARD 
GREENS,  NOVEMBER  20,  1975 

Hours  of  Operaclon 


Hours  of  Operacloo 


U.O 
0.1 


100.0 
18.0 


100.0 
32.0 


All  values  replicated.   Colonies  were  991  Bacillus  subtllls. 


TABLE  C-5.   TOTAL  PLATE  COUNT  (COLONIES  X  10  PER  MILLILITER)  IN  WASH 
WATER  FROM  PROTOTYPE  FOR  TRIAL  5.  FALL,  WASHING  OF  SPINACH 
(3f£ENS.  DECEMBER  15.  1975 , 


36. 0" 
18.0* 

0.2= 
0.2 
16.1 


Average  of  two  values. 


3 


3.9 

7. a" 


9.2' 
9.4" 
2.8' 
2.9» 
6.6' 
14.0 


Hours  of  Operation 


TABLE  C-6.   TOTAL  PLATE  COUNT  (COLONIES  X  10  PER  MILLILITER)  IS  PROTOTYPE 

AND  CONVENTIONAL  WASH  WATER.  TRIAL  1,  SPRING,  WASHING  OF 
SPINACH  GREENS.  APRIL  21.    1976 


.3.0" 

>3.0' 

2.7 

0.04' 

0.04 

>3.0" 

Hours  of  Operation 


8.4" 
13.9' 

5.8» 
24.9' 

3.0' 
16.5* 


15.5- 
10.1' 
3.2' 
4.9' 
5.0° 
12.9° 


0.3 

- 

90.0 

- 

25.4 

0.2 

- 

7.0 

- 

5.3 

12.0 

14.6 

- 

700.0 

- 

3.6 

4.4 

- 

1860.0 

- 

'Average  of  cwo  values.    "insuf f icienc  dilutions  before  plating. 


TABLE  C-7.   TOTAL  PLATE  COUNT  (COLONIES  X  10  PER  MILLILITER)  IN  WASH 

WATER  OF  CONVENTIONAL  SYSTEM  FOR  TRIAL  3,  SPRING,  WASHING 

OF  SPINACH  GREENS,  MAY  21.  1976 


Hours  of  Operation 


TABLE  C-a.   TOTAL  PLATE  COUNT  (COLONIES  X  lo'  Pl.R  MILLILIIFR)  IN  rROTOTYPE 
AND  CONVENTIONAL  WATER  FOR  TRIAL  4.  SPRING.  WASHING  OF  TURNIP 


1 » 

Hours  of  Ope 

ration 

site 

.25 

2 

3 

5.5 

1 

>30.0' 

- 

200.0 

30.0' 

2 

>30.0' 

- 

450.0 

300.0" 

4 

.30.0' 

- 

390.0 

600.0 

13 

>30.0* 

63.0 

- 

38.0 

15 

>30.(V' 

85.0 

31.0 

'Avetags  of  two  values. 


'insufficient  Dilution. 


84 


TABLE  t-9.      TtTTAI,   riATf.  CdUMT    (rjlUWlfS   I    10 '   ri«  Mll.l.l  I.ITKI)    III   MCOTOTTPE     TAIIJ  C-IO.      TOTAl.  PUTT.   CUUfT    (■.'OURIIES  X    lo'  PKt  NIIXILITa)    l«  PMITOnn 

AHD  COHVarriOIIAL  UATLII    ro«  THIAl.    S.    SPKlHi;.    MASIIIIir.  or  TUWIP                                      AND  COUVDtTlOIIAL  yATtH    ITMI  TilAL  (..    SPHtHC     ■AUIIB  0»  Tvnir 
CHEEKS,    JWE    10.    1976 -       i»p»ir 


Hour*  of  OpTatlon 


9.2 

- 

26.0 

24.0 

».7 

- 

2*.0 

3S.0 

2.S 

- 

1.2 

40.0 

13 

3S.0 

220.0 

55.0 

- 

15 

ti.O 

300.0 

39.0 

- 

Houra 

ot  Oxra 

lion 

""       ... 

.2> 

2 

4 

6 

1 

M.S 

- 

25.0 

2).0 

2 

IM.O 

- 

SJ.i 

11.0 

A 

- 

- 

?.5 

6.0 

IJ 

A9.0 

290.0 

160. 0 

- 

IS 

70.0 

IJO.O 

260.0 

" 

TABU  C-U.      TOTAL  roLlKOIlM  COUNT    (COLONIES    X    lo'    PE«  MlLLlLlTt«)    IN  WASH 
HATER   rHOII   PHOTOTYPE   ro«  TRIAL   1.    FALL.    HASH  INC  OF  COLLA«D 
CBEEMS.    OCTOBER  24,    1975 


1.00 
0.32 


Hour«  of  Operation 


>3.ao 

0.02 

0 

M.O 


TABU  C-12.      TOTAL  COLIPORH  COUNT    (COLONIES   I    lo'   PU  MILLILITER)    IH  HASH 

HATER    rtM  PROTOniE    FOR   TRIAL   2,    PALI..    HASIIIHC  OF  COLLARS 
(jREtMS.   MOWER  4.    1975 


0.25 


0.01 

O.JI» 

O.Jl" 

7.n» 


Hwifa  of  Operation 


IS.M 
11.7S» 


39.00 


90.00 
6.15' 
1.00 
5.10" 


'insufficient  dilution  before  plating. 


Avvrage  of  two  vaiuon. 


of    tlirrc  v.iltics. 


TABLE   C-n.      TOTAL   CoHFOWl  COUNT    (COLONIES   X    10      Pt«   MlLLlLlTtR)    IN    HASH 

HATER   mOM  PROTOTYPE    FOR  TRIAL    3,    FALL,    HASHING  OF  COLLARD 
CKEENS,    NOVEMBER  20.    1975 


Houra  ot  Operation 


14.00- 

o.os 


4.00 
22.00 


^Average  of   three   values. 


TABU  C-15.      TOTAL  COLIFORM  COUNT    (COLONIES   X   10      PER  MILLILITER)    IN  HASH 

HATER  FROM   PROTOTYPE    FOR  TRIAL    5.    FALL.    HASHINC  OF  SPINACH 
CREENS,    DECEMBER    15,    1975 


Hours  of  Operation 


3 


>ip.oo 

>i0.oo'' 

>io.oo'' 


1.20 
2.»0» 

14.00 
21.60" 


"Average  of   tvo  values.  Insufficient   dilutions  before  plating. 


TABU  C-14.      TOTAL   OOLIFURM  COUNT    (COLnMlfS    X    10      PER  MILLILITER)    lU  HASH 

HATER    FROM  PROTOTYPE   FOR  TRIAL    i.    FALL.    HASHING  OF  COLLARD 
CRKBiS_L  DECQBER    1.    1975    


Hours  of  Operation 


33(...l" 
174.0'' 

1.." 

2.0" 

134.0 


39. 0' 


w.r 

29.0* 


Average  of  two  values.      Aver.-icc  of  (our  vjlues.    ''Average  of  five  values. 

TABU  C-10.   ANALYTICAI.  CCMCKNTR.U10NS  (•f/c)  OP  TOTAL  SUSI'KHDED  SOLIDS, 
TRIAL  1.  FALL.  HASHING  OF  COLLARD  (a£EMS.  OCTOBER  24.  1975 


Houra  of  ilpcratlon 


10" 
M 

»" 

2 

r' 

2 


79* 
132 
9' 

4 

12" 
64 


10»' 
120 

17" 
2 

10 
112 


91 

100- 
4i" 
12 
»■• 

us" 


109" 

152" 

20 

14 

40 
124 


Average  of  two  valu«s. 


85 


T*BLE   C-17. 

ANALYTICAJ.   CONCtNTRATlONS    (he/D    UF  TOTAL   SUSPENDED   SOLIDS. 
TRIAL  2.    FALL.    UASHINC  OF  COLLARD  CRKENS.   NOVEMBER   4.    1975 

Hours  of  Operation 

Sice 

0.25                  12                  3                  4                  5                  6 

TABLE  C-18.      ANALYTICAL  CONCENTRATIliNS    (mc/O    UP  TOTAL  SUSPENDKD   SOLIDS, 


2»' 

32 

20 

10 

!•• 

25 

58" 


92 

92» 

119 

220' 

120" 

128 

155* 

180» 

24 

29« 

22 

64 

44» 

36 

58 

72 

12 

44^ 

60 

76 

134" 

116 

145" 

210» 

* 

-I 

Sii i — "   "^ 

jy_j — iji  J 

Hours  of 

Operation 

Site 

1 

3 

5 

7 

52- 

26' 


Average  of  two  voiues. 


240 
68 


156 
225 


Average  of  two  values. 


TABLE  C-19.   ANALYTICAL  CONCENTRATIONS  (»g/t)  OF  TOTAL  SUSPENDED  SOLIDS, 
TRIAL  4,  FALL,  WASHING  OF  COLLARD  GREENS,  DECEMBER  1,  1975 

Hours  of  Operation 


16' 


7 
10 
10 
58 


,62 

69' 

68= 

94 

15 

22 

10 

37' 

18 

31 

63" 

96 

TABLE  C-20.   AHALYTICAL  CONCENTRATIONS*  (mg/t)  OF  TOTAL  SUSPENDED  SOLIDS, 
TRIAL  5,  FALL.  WASHING  OF  SPINACH  GREENS.  DECEMBER  15,  1975 


Hours  of  Operation 


184 

166 

148' 

200 

230" 

184 

214" 

232" 

33 

47 

42 

44 

41 

56" 

46" 

36" 

48 

60 

48 

20 

211" 

196 

184" 

200 

6 

0 

1 

2 

3 

4 

39 

384 

720 

752 

689 

32 

491 

802 

902 

632 

16 

112 

199 

224 

227 

29 

137 

217 

274 

280 

18 

121 

207 

274 

274 

41 

456 

766 

902 

712 

Average  of  two  values. 


Average  of  two  values  in  each  case. 


TABLE  C-21    AHALYTICAL  CONCENTRATIONS  (mg/l)  OF  TOTAL  SUSPENDED  SOLIDS. 
TRIAL  1.  SPRING.  WASHING  OF  SPINACH  GREENS.  APRIL  22.  1976 


3 

4 
5 
6 
7 
8 
9 
10 


69 
78 
80 
347 
1321 
1277 
449 
445 


Hours  of  Operation 


637 

1069 

98 

100 

112 

960 
2188 
2175 
1219 
1156" 


1057 

1537 

351 

371 

361 

1485 

1252" 

1145" 

849' 

680" 


3 


1213 
1673 
514 
559 
543 
1680 
199 
265 
544 
471 


913 
533 
475 
158 
480 
910 
295 
239 
366 
379 


797 
1130 
337 
369 
358 
1101 


750 
690 
277 
333 
289 
827 


938 
1280 
318 
397 
320 
1104 


TABLE  C-22.   ANALYTICAL  CONCENTRATIONS  (mg/!)  OF  TOTAL  SUSPENDED  SOLIDS, 
TRIAL  2.  SPRING,  WASHING  OF  SPINACH  GREEHS,  MAT  12,  1976 

Hours  of  Operation 

Site         2        3 4 5 6 7 T^J 


9 
10 


2596" 

6830" 

1354 

1047" 

46S" 

953" 

950 

2464" 

8306" 

1386 

996" 

455" 

1022" 

929 

I760" 

3:eo" 

24:4' 

1U7  7'' 

515" 

951" 

1026 

1612" 

3716" 

2195" 

1041" 

4  36" 

851" 

985 

Average  of  two  values. 


TABLE  C-23.   ANALYTICAL  CONCENTRATIONS  (me/n  OF  TOTAL  SUSPENDED  SOLIDS. 
TRIAL  3.  SPRING,  WASHING  OF  SPINACH  GREENS.  MAY  21.  197& 


Hours  of  Operation 


Average  of  cwo  values. 


12 

162 

343 

469" 

523 

446' 

13 

158 

423 

304" 

490 

419' 

14 

128 

356 

321 

446" 

376" 

15 

121 

311 

280 

449" 

416' 

Average  of  two  values. 


86 


TABLE  C-24.      A1IA1.YTICAL   COKCENTRATIOIl    (mg/t)    OF  TOTAL  SUSPENDED   SOLIDS, 


TABLE   C-25.      ANALYTICAL  C0NCEN1  RATIONS    (tnR/O    OK  TOTAL   SUSPENDED  SOLIDS. 
TRIAL    5.    SPmNC,    UASHIWC  OF  TURNIP  CREENS.    JUNE    10,    1976 


Hours  of 

Operation 

Site 

Hours 

of  Operation 

Site 

0.25 

I 

2 

3 

4 

5.5 

578* 

0.25 

1 

2 

3 

4   _ 

274 

5 

6.5 

1 

lis 

367 

399 

440* 

588* 

1 

66 

166 

153 

410° 

158 

132 

t 

324* 

530" 

4  76° 

608* 

635* 

2 

94* 

210 

200 

5)0* 

33) 

187 

155 

3 

67 

221 

212-' 

275° 

32b* 

3 

27 

75 

40 

160 

149 

101 

68 

4 

117 

200 

258" 

280' 

329* 

4 

27 

85 

40 

16S 

110 

69 

72 

S 

103 

2  38 

230° 

316* 

339* 

5 

23 

77 

90 

120 

15« 

65 

157 

« 

123 

49»' 

494* 

603° 

635* 

6 

87 

152 

229 

4  71° 

)24 

184 

154 

12 

S3 

230 

119 

81 

- 

12 

31 

82 

133 

94 

88 

- 

- 

13 

62 

227 

166 

125 

- 

13 

39 

76 

148 

106 

89 

- 

- 

14 

S3 

185 

115 

79 

- 

14 

21 

52 

76 

74 

54 

- 

- 

15 

54 

164 

119 

72 

15 

26 

40 

79 

59 

48 

~ 

~ 



Average  of    two  values. 


'Average  of   two  values. 


TABLE  C-26.      ANALYTICAL  CONCENTRATIONS    ('S/t)    OF  TOTAL    SUSPENDED  SOLIDS,  ANALYTICAL  CONCENTRATIONS    (mg/f)    OF  VOLATILL    SUSPFJIDED  SULIUS. 
TRIAL   6.    SPRING,    WASHING  OF  TURNIP   GREENS,    JUNE    U,    1976              TRIAL    1,    FALL.    MASHINC   m    COLLARD  CHLENS .    OCTUbF.R    24.    1975. 


Hours  of   Operation 


Hours  of  Operation 


1 
2 
3 

4 

s' 

6 
12 
13 
14 
IS 


138 
216 

45 

62 

50 
214* 

41 

37 

22 

23 


204 
290 

68 

91* 

70 
2  78 

43 

50 

32 

40 


376- 

461° 

129° 

141 

139 

476° 
45 
52 
47 
40 


*Average  of   two  values. 


365- 

4)0* 

1)4 

142 

137 

414 
79 
71 
46 
59 


179 
224 
79 
91 
84 
226 
77 
75 
59 
84 


166 
213 


182 
232 
89 
92 
88 
220 


17" 

36" 

38 

61' 

44 

46 

50 

70' 

15' 

13* 

15* 

14 

2 

4 

18 

34 

12* 

18 

20* 

34 

28 

3)" 

53* 

80 

Average  of  two  values. 

TABLE  C-28.      ANAI.YTICAI.  rONCrNTHATlON*;  (hir/O  OF  VilJ.ATll.F:  SnSPr.NIIKR  SOLIDS. 
TKIAL  2.  FALL.  HASIIINC  UK  COLLAKO  GKEUNS.  NOVEHBrB  U.    1975. 

Hogrs  of  Opcrntton 


13" 

20 

7 


27 
36* 
9 
15* 


32 
39* 


155 
145" 


Avor.ige   o(    twn   valued 


87 


TABLE  C-29.      AHALYTICM,  CCWCmTRATIONS    (m»/0    OF   VOLATILE  SUSPWOFD  SOLIDS,  lABLF.   C-IO.      ANALYTICAl.  CONCENTRATrwiS    (ait/l)   OF    VOLATFIF   SUSrF.HDtO  SOLIDS. 
TRIAL   3,    FALL.   UASHINr.  OF  COUMW  r.»t.e>IS.    HOVrFgH    ZO,    1975 TBIAL   «.    FALL.   WASIIIMC  OF  COLLAM)  r«F.tNS .    UFI  FMI\F»    I.    1975 


Hours  of   Op«r«clon 


Hours  of  Operation 


46" 
2»' 


*Averase  of  two  values 


106 
120 


120 
SO 


108 
150 


17- 

124 

27* 

17' 

6 

12 

- 

10 

10 

15 

20 

36' 

36 

70 

7b 

82* 

114' 

48 

84' 

lOO 

94' 

lOo' 

8 

24 

27 

32 

20 

33* 

26 

38' 

37' 

14' 

21 

27 

36 

44 

- 

TABLE  C-31.   ANALYTICAL  CONCENTRATIONS  (mg/t)  OF  VOLATILE  SUSPENDED  SOLIDS. 
TRIAL  5.  FALL.  UASHINC  OF  SPINACH  QUEENS.  DECEMBER  15,  1975 


Averaee  of  two  values. 


Hours  of  Operation 


•  TABLE  C-32.   ANALYTICAL  CONCENTRATIONS  (ag/t)  OF  VOLATILE  SUSPENDED  SOLIDS, 
TRIAL  1,  SPRING,  WASHING  OF  SPINACH  GREENS,  APRIL  22.  1976. 


52 
1) 
It 

18 

45 


66 
69 
19 
31 
23 
68 


61 
6i 
17 
2$ 
29 
54 


32 
20 
27 
29 
40 


Houra  of  Operation 


Single  values,  all  others  are  average  of  two  readings. 


TABLE  C-33.   ANALYTICAL  CONCENTRATIONS  («g/l)  OF  VOLATILE  SUSPENDED  SOLIDS, 
miAL  2.  SPRING,  UASHINC  OF  SPINACH  GREENS,  MAY  12.  1976 

__^ Hours  of  Operation 


Site 
1 
2 
3 
4 
5 
6 
7 
8 
9 
10 


26 

36 


40 
134 
136 


68 

112 

182 

180 

144 

153 

150 

103 

164 

257 

92 

188 

131 

188 

14 

41 

74 

91 

70 

55 

55 

17 

44 

88 

11 

72 

61 

58 

26 

44 

82 

93 

72 

60 

54 

90 

160 

259 

183 

20o 

156 

163 

241 

123' 

22 

62 

- 

- 

- 

228 

114' 

25 

54 

- 

- 

- 

144 

106" 

58 

71 

- 

- 

- 

137' 

7  7' 

52 

71 

_ 

. 

. 

7 

8 

9 

10 


190' 

306' 

138 

156' 

55 

81' 

77 

177' 

521' 

160 

152' 

65 

84' 

77 

161' 

2  78' 

200' 

165' 

72 

90' 

85 

145' 

267' 

184' 

164' 

70 

86' 

86 

Average  of  two  values. 


TABLE  C-35.   ANALYTICAL  CONCENTRATIONS  (hk/D  OF  VOLATILt  SUSPt.NDED  SOLIDS, 
TRIAL  4,  SPRING.  WASHINL  OF  TURNIP  GREENS.  JUNE  4,  1976 


Hours  of  Operation 


Average  of  two  values. 


TABLE  C-34.   ANALYTICAL  CONCENTRATIONS  (niB/t)  OF  VOLATILE  SUSPENDED  SOLIDS, 
TRIAL  3,  SPRING,  MASHING  OF  SPINACH  GREENS,  KAY  21.  1976 


12 
13 
It 
IS 


20 
21 
20 
IS 


Hours  of  Operation 


60 
67 
67 
62 


64* 
50" 
57 
55 


95 
8) 
93* 
85* 


6 
12 
13 
14 
15 


18 

36' 

10 

19 

15 

34 

12 

12 


32 

28 
51 
15 
20 
21 
17 


45 

6  3'^ 

29 

30 

35" 

61^ 


38" 
35' 


34» 
36' 


18 

15 

1 

15 

20 

13 

42° 
7l' 


Average  of  two  values. 


Average  ut    two  values. 


88 


TABLE  C-3«..   ANALYTICAL  CONCENTRATIONS  (ruR/t)  OF  VOLATILE  SUSPENDED  SOLIDS,  TABLE  C-37.   ANALYTICAL  CONCENTRATIONS  (cg/l)  OF  VOLATILE  SUSPENDED  SOLIDS, 
TRIAL  5,  SPRING.  HASHING  OF  TURNIP  GREENS,  JUNE  10,  1976 TRIAL  6.  SPRING,  UASHINC  OF  TURNIP  GREENS.  JUNE  11,  1976 


Niiura  or  Oncratlon 


lie 

0  ._25_ 

1 

2 

5 

1 

7 

18 

17 

64'' 

26 

2 

12^ 

28 

26 

..a 

30 

1 

A 

8 

- 

12 

4 

8 

12 

- 

13 

5 

1 

11 

11 

12 

6 

6 

14 

34 

j.a 

28 

12 

11 

20 

21 

- 

13 

15 

15 

19 

- 

14 

13 

25 

21 

- 

15 

12 

17 

20 

- 

.  Jl^ur^.  J^  Oi>e ration 


llj. 

0.25 

.-.I 

2 

25 

49 

56" 

39 

73 

102-^ 

9 

17 

2)' 

14 

21" 

23 

U 

17 

22 

41" 

85 

64" 

12 

8 

7 

9 

13 

8 

8 

8 

14 

10 

11 

9 

IS 

9 

10 

8 

28 
29 


2J 

25 


24 

15 

14 

15 

23 

15 

16 

15 

54" 

26 

25 

25 

Average  of  two  values. 


Average  of  two  values. 


TABLF.  C-38. 

ANALYTICAL  CONCENTRATIONS  (mg/1)  OF  ClltMICAL  OXYCFN  DEHAND, 
TRIAL  I,  FALL.  UASIIINC  OF  tOLLAW)  CREtNS.  OCTOBER  24.  1975 

Hours  of  Operation 

Sice 

0.25           12            3         4 

26 
13 
10 


91 
132 
33 

47 
45 
110 


136 
169 
43 
57 


173 
207 
69 
96 
93 
206 


TABLE  C-39. 

ANALYTICAL  CONCENTRATIONS  (mg/I)  OF  CHFMtCAL  OXXC.F.K   DEMAND. 
TRIAL  2.  FALL.  UASHINC  OF  COLLARD  GREENS.  NOVEMBER  4.  1975 

Hours  of  Operation 

Site 

0.25        1        2        3        4        5        6 

262 

1 

146 

2 

103 

3 

144 

4 

132 

5 

303 

6 

45 
49 
25 
33 
31 


92 
129 
35 


214 
59 


75 
192 


304 

355 

430 

419 

349 

453 

492 

428 

98 

13] 

172 

103 

121 

123 

209 

219 

117 

148 

201 

212 

155 

194 

414 

557 

Single  value,  all  others  are  average  of  two  values. 


Average  of  tw 


TABLE  C-40.   AN\LYTICAL  CONCENTRATIONS  (mp/f)  OF  CIIFHtCAl.  OXYGEN  DEMAND. 
TRIAL  1,  FALL,  UASHINC  OF  C0LI.AKD  GKtXNS,  NOVEMBER  20,  1975 

Hours  of  Operation 

Site  1  3  5 7 


149 
93 


378 
186 
409 


458 
194 


Avpr.igc  of  two  v,ilues  in  each  rase 


400 
264 


lABlE  C-42.   ANALVTICAI.  CONCKNTRATIONs'  (mg/l)  OF  ClltMlCAL  OXYGIN  DEMAND, 
TRIAL  5,  FALL.  UASHINC  OF  SPIWACU  GREENS,  DECEMBER  15.  1975 

Hours  of  Operation „ 


TABLE  C-41.   ANALYTICAL  CONCENTRATIONS  (mg/l)  OF  CHEMICAL  O.XYGEN  DEMAND, 
TRIAL  4.  FALL.  HASHING  OF  COLLARD  GREENS,  DECEMBER  1,  1975 

^ Hours  of  Operation 


24 

86 

96 

204 

244 

275 

292 

321 

26 

140 

158 

214 

290 

315 

359 

167 

20 

16 

192 

67 

81 

67 

106 

112 

28 

58 

l,l< 

89 

108 

no 

111 

210 

24 

40 

66 

91 

87 

1  17 

127 

127 

29 

42 

218 

220 

2(.5 

290 

110 

141 

Average  of  two  valuea  in  each  case. 


25 
25 
25 
16 
31 
35 


113 
115 
62 
63 


171 

194 

79 

112 


200 
240 
98 
129 
130 
240 


211 
246 
95 
113 
115 
214 


"Average  of  two  values  in  each  case. 


89 


TAIL!  C-«3.      ANAUTICAL  CONCENTRATIONS    (ii|M)    OF  CIICHICAL  OXYOIN   DUMND, 
THIAL   1.    aniNC.   WASHIKC  07  SPIWACM  CMENS.   APML  22.    H7t 


TABLE  C-«4.      ANALYTICAL  CONCEHTKATIUNS    (m|/t)   Or  ClIIIHICAL  OXYCLN   DEHAND, 
miAL  2.   SrRINC.   WAJHINC  Of  SUMACH  GREENS.   HAY   12.    197t 


Houri  ot  Operation 


Houf  of  Oporatlon 


1 
2 
3 
4 
S 

e 

7 
8 
9 
10 


0.25 


82* 
89'' 
U» 
63 

109 

lit 

274 

261' 

117 

in" 


161" 
194* 
106 
124 

72' 
318* 
506 
514 
247 
277" 


313 

354 

76 
136* 
105 
343* 
271* 
204 

97* 

96 


433*  463* 

579  765^ 


173 
203* 
183 
563* 

49 

53 

69 


220 

347* 

229 

641° 

164* 

168" 

155 

134 


431- 

538° 

170* 

185* 

181 

414* 


388 

509* 

132 

164* 

145 

492* 


; 


378 

464 

136 

168* 

152 

492* 


9 
10 


7 


280* 

557° 

154° 

2  79* 

113-' 

221° 

210' 

222 

724 

139 

249 

87 

208 

202 

277* 

386* 

267* 

247* 

128 

192* 

205' 

236 

385 

301 

274* 

111 

149 

190' 

Average  of    two  value* , 


TABLE   C-45.      ANALYTICAL  CONCENTRATIONS    (i»g/«)    OF  CHEMICAL   OXYCtN    DEHAND, 
TRIAL    3.    SPRING.    WASHING  OF   SPINACH   GREENS,    MAY    21.    1976 


Site 

.25 

1 

3 

»e  nf 

two  valuea. 

3.25 

*Aver 

12 

80* 

237 

252* 

309° 

304° 

TABLE 

C-46. 

ANALYTICAL 

CONCENTRATIONS  (lug/l)  Ot 

■  CHEMICAL 

OXYGEN 

DEMAND 

13 

14 

85 
82* 

236 

219 

190* 
213° 

321 
308° 

349 

TRIAL  4,  SPRIHC^  WASHING 

OF  TURNIP 

GREENS, 

JUNE  4.  1976 

a 

375° 

lloura  of  Operation 

IS 

71 

222 

186* 

301 

329 

Slce 

0.25 

1 

2 

4 

5.5 

60 
77* 

141 
152" 

163 

200* 

269* 

255 
282* 

288 
316* 

'Average  ut 

two  val 

UL'B. 

43 

N 

102 

149 

204 

45" 

82" 

108* 

1S1° 

159* 

205* 

TABLE  C-47 

.   ANALYTICAL  CONCENTRATION  (og/t)  OF  CHEMICAL  OXYGEN  DEMAND, 
TRIAL  5,  SPRING.  WASHING  OF  TURNIP  CRtENS,  JUNE  10.  19  76 

47 

94 

114 

157 

208 

u 

13 

87* 
SO* 
63 

141" 

141 

145 

190* 
185° 
188 

256* 
167° 

283* 
137* 
125 

306° 

Hours  of 

Operatl 

on 

Site 

0.25 

1 

2 

3 

4 

5 

6.5 

- 

1 

32 

78* 

155° 

216* 

151* 

155 

135* 

It 

67" 

192 

263* 

7n* 

190* 

- 

2 

28 

102 

157 

220 

178 

149 

153* 

IS 

61 

194 

259 

190 

- 

3 
4 
5 
6 

19* 

16 
16° 

44 

45° 
18 
38* 
98 

106° 

63 

60* 
149 

122° 
118 
119° 
243 

72* 
82 
87° 
169 

61° 
67 
55° 
145 

67 
63 
159° 

141 

*Aveiag«  of 

two  valuea. 

12 

65* 

88° 

199* 

193° 

186° 

- 

- 

13 

49* 

69 

200 

184 

180° 

- 

- 

14 

40* 

133° 

295* 

225° 

228° 

- 

- 

13 

41 

92 

235 

222 

204 

_ 

_ 

Average  of   two  valuai. 


90 


TABLE  C-4H.      ANALYTICAL   CONCENTRATION    (mg/t)    OP  CllfcHICAL  OXYCbN   DEHAHD, 
TR I AL_  /jj_  SPRING,    WASHIWti   UF^  fURN IJJiW.MH ,    J UNE__Ujl  J9±b 


TABl.t  C-69.      ANAI.YTfCAL  CtWCKNTRATIdNS    (mg/O    OV   FIVK-I>AY    HItK;tli:MH:AI. 
nXYUKN   nKHANI).    TKIAl.    I.    VALL,    WASItINC  OF  CUl.LAKU  i:k>.KNS, 


lltMirw   of    li|ifi(il  Iti 
2  3 


li.i^u(j»_  iJ__up_»miii_op__ 


1 

92  = 

1B5' 

194° 

178' 

114' 

104" 

112' 

2 

144 

240 

228' 

200 

US' 

120 

124 

3 

38' 

68' 

92 

98' 

61' 

56' 

65' 

4 

44 

80 

104 

98' 

66 

57' 

70 

5 

47  = 

79' 

122' 

90' 

66' 

55' 

72* 

6 

136 

240 

228 

200 

124 

116' 

130' 

12 

36' 

64' 

93' 

89' 

115' 

- 

- 

13 

36' 

60 

99' 

74 

115' 

- 

- 

U 

35' 

86' 

133' 

112' 

157' 

- 

- 

15 

32 

76 

92' 

'>2 

166' 

Average   of    two  values 


Average  of    three  values. 


sit. 

1 

2 

4 

1 

28 

31 

8I' 

2 

17 

34' 

89 

3 

9 

8 

20" 

i 

10 

15' 

36' 

5 

18" 

10 

36' 

Average   of    two  values. 


TABLE    C-51.       ANALYTICAL  CONCENTRATIONS    (m%/l)    OF    F1VK-0AY    BIOiHI.MlLAL 
OXYCtN    OEMAWD.    TRIAL    3.    FALL.    WASHING   OF  COLLARD  CRttNS, 
NUVLMBER   20.    1975 


TABLE   C-50.      ANALYTICAL  CONCENTRATIONS    (mg/l)    OF   FIVL-DAY   BIOCHtHICAL 
OXYGEN   DEHAND,    TRIAL   2,    FALL.    WASHING  OF  COLLARD  GREENS 
NOVEMBER  ^.    1975 


Hours  of  Operation 


lliL 

Hours  ot 

Operation 

site 

0.25 

1 

2 

3 

4 

5 

6 

8 

24' 

44 

62 

95' 

108 

97 

11 

30 

45 

88 

89' 

134 

103' 

4 

8 

11' 

24' 

28 

35 

20' 

3 

5 

12 

25 

18 

35 

59 

4 

6 

8 

21' 

27' 

47 

46 

10 

21 

50 

87 

114 

98 

91' 

Average   of    two   values,    other   values   are   average   of    three. 


TABLE  C-53.      ANALYTICAL  CONCENTRATIONS    (mg/O    OF   FIVE-DAY    BIOCHEMICAL 
OXYGEN   DEMAND,    TRIAL   5.    FALL,   WASHING  OF   SPINACH  GREENS. 
___^ DECEMBER   15,    1975 


Hours  of  Operation 


Slf 

e                                 1 

2 

3 

4 

1 

30" 

49'' 

44" 

46" 

30" 

52' 

57' 

39* 

5- 

24*' 

25"= 

22* 

i 

»' 

21' 

Si" 

22* 

»• 

22'' 

32' 

19" 

( 

21* 

^^ 

Si' 

32"= 

Average   oC    two   values. 


TABLE   C-52.      ANALYTICAL  CONCENTRATIONS    (ng/!)    OF   FIVE-DAY   BIOCHEMICAL 
OXYGEN    DEMAND,    TRIAL   4,    FALL,    WASHING  OF  COLLARD  GREENS. 
DLCLUBER    1.    1975 

Hours   u(    Operatltin 


27 

50 

60 

31' 

52" 

71' 

12' 

14' 

19' 

14'' 

15' 

22' 

75 

88' 

76 

91' 

116' 

97 

22' 

30' 

35 

30' 

36' 

39 

39 

42 

38 

99' 

101' 

24 

Average   of   two   v.tIucb.      Average   of    three  values.    '^Average   of   four   values. 


"Average  of   two  values.         ''Average  of    three  values.      ^Average  of    four  vdJues. 


91 


TAILE  C-St.      ANALVTICAL  COCUITIUTIONS    (ag/I)    OF   ZO-DAV    BIOOIEmCAL  OnCEM 
DUIAMD,    TlltAL    1,    FALL,    UASHIHC  OF  COLLARS  CUENS,    OCTOIER   2i, 
H75 


Houf  of  Optratton 


tHe «.0 

1  99 

2  lOS 
1  10 
»  21 
i  M 
«  »] 


lAlU  C-55.  AKALYTICAL  COHCtHT HAT IONS  (■«/')  OF  20-DAV  BIOCHEMICAL  OXYGEN 
DCHAND,  T«UU.  2,  FALL,  UASHIHC  OF  COLLARD  UREEHS,  NOVEMBER  4, 
1975 


Uoura  of  Opgration 


Average  o(    two  v.ilu«-9. 


TABLE  C-S«.      AHALVTICAL  COMCENTRATIONS    (•(/!)    OF   20-DAV    BIOCHEMICAL  OXVCEN 
DEHAMD  TRIAL  *■   FALL.   WASIUHC  OF  COLURP  (■.Rt:tJIS.   DECEMBER  1.    197} 


Houri  of  Opration 


1  35" 

2  12  S0° 

3  It  10* 
«  -                                                      13" 

5  »" 

6  0  J?*" 

'Avaraga  of   two  valuea.        Avaragc  of  threr  valuea.       *^Avcrage  of   four  values. 


TABLE  C-il.      AHALVTICAL  COHCRMTRATIONS    (•«/>)    OF  20-l>AY    BIOOILMLCAL  OXVCEN 
DEHAHII.   TRIAL  5.    FALL.   MASHING  OF  SPIHAOI  CKtEHS.    DFX . .    IS.    1975 


Houra  of  Oparatlon 


1»* 

3J' 

41" 

»»" 

15* 

37' 

w-^ 

m" 

12* 

6* 

2." 

30' 

17* 

13* 

30" 

»3« 

17* 

12* 

27* 

«' 

13* 

31" 

62"= 

J7' 

Awrat*  **'    '^'O  valuua.  Avcrat*  ot   thrae  valuon.  Avorngc  uf    four   valuaa. 


92 


TECHNICAL  REPORT  DATA 

(Please  read  Inuructions  on  the  reverse  before  completing) 


1.  REPORT  NO. 

EPA-600/2-77-135 


3.  RECIPIENT'S  ACCESSIOI*NO. 


4.  TITLE  AND  SUBTITLE 


5.  REPORT  DATE 

July  1977  issuing  date 


Minimization  of  Water  Use  in  Leafy  Vegetable  Washers 


6.  PERFORMING  ORGANIZATION  CODE 


7.  AUTHOR(S) 

Malcolm  E.  Wright 

Robert  C.  Hoehn  (Civil  Engr.  Dept.  -  VPI  &  SU) 


8.  PERFORMING  ORGANIZATION  REPORT  NO. 


9.  PERFORMING  ORGANIZATION  NAME  AND  ADDRESS 


Agricultural  Engineering  Department 

Virginia  Polytechnic  Institute  &  State  University 

Blacksburg,  VA  24061 


10.  PROGRAM  ELEMENT  NO. 
1BB610 


11.  CONTRACT/GRANT  NO. 

S-802958 


12.  SPONSORING  AGENCY  NAME  AND  ADDRESS 

Industrial  Environmental  Research  Lab 
Office  of  Research  and  Development 
U.S.  Environmental  Protection  Agency 
Cincinnati,  Ohio   45268 


-  Cin. 


OH 


13.  TYPE  OF  REPORT  AND  PERIOD  COVERED 

Final     5/1/74   -   1/31/77 


14.  SPONSORING  AGENCY  CODE 


EPA/600/12 


IS.  SUPPLEMENTARY  NOTES 


16.  ABSTRACT 

This  project  was  undertaken  to  construct  and  test  an  improved  leafy  greens  washing 
system  employing  water  recirculation,  to  characterize  the  quality  of  the  wash  water 
and  waste  stream  and  to  make  comparisons  to  conventional  washers.   The  prototype 
system  produced  a  cleaner  product  while  reducing  water  requirements  and  consolidating 
waste  loads.   The  prototype  system  consisted  of  two  drum  immersion  washers  in  series, 
each  with  associated  moving  belt  screens,  settling  tanks  and  water  recirculation 
systems.   Construction  was  similar  to  conventional  washers  but  with  modifications  to 
improve  removal  of  floating  trash  and  increase  hydraulic  agitation  of  product.   The 
prototype  was  tested  in  a  commercial  processing  plant  during  the  fall  and  spring 
harvesting  seasons,  1975-76.   Sixty-seven  metric  tons  of  collards,  spinach,  and 
turnip  greens  were  processed  through  the  prototype  in  52  hours  of  actual  operating 
time.   Conventional  washers  were  monitored  for  27  hours  (38  tons)  for  comparison. 
Insect  and  bacteria  counts,  COD,  TSS,  VSS,  and  several  other  water  and  product 
parameters  were  measured  at  predetermined  times  and  locations.   Data  were  obtained 
to  predict  expected  waste  loads  from  the  products  processed.   Wastewater  discharge 
from  the  prototype  was  approximately  1/12  that  of  the  conventional  washers. 


17. 


KEY  WORDS  AND  DOCUMENT  ANALYSIS 


DESCRIPTORS 


b. IDENTIFIERS/OPEN  ENDED  TERMS       C.      COSATI  Field/Group 


Food  Processing,  Circulation,  Canneries, 
Freezers,  Water  Quality 


Leafy-vegetable  process- 
ing, Process  modification 
Washing  systems.  Water 
reuse 


13/B 


IS.  DISTRIBUTION  STATEMENT 


Release  Unlimited 


19.  SECURITY  CLASS  (This  Report) 

UNCLASSIFIED 


21.  NO.  OF  PAGES 
105 


20.  SECURITY  CLASS  (This  page) 

UNCLASSIFIED 


22.  PRICE 


EPA  Form  2220-1  (9-73) 


93 


,v  U.  S   GOVERNMENT  PRINTING  OFFICE   1977-757-056/6'.99  Region  No.    5-11 


J 


TX  601  .Un3 


2^fC 


United  States.  Environmental 
Protection  Agency. 

Minimization  of  Water  Use  in 
Leafy  Vegetable  Washers. 

DATE    I  ISSUED  TO 


TX  601  .Un3 


e.s/(^ 


United  States.  Environmental 
Protection  Agency. 

Minimization  of  Water  Use  infers 
Leafy  Vegetable  Washers. 


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