BENTON HARBOR POWER PLANT LIMNOLOGICAL STUDIES PART IX. THE BIOLOGICAL SURVEY OF 10 JULY 1970 John C. Ayers William L, Yocum H. K. Soo Thomas W. Bottrell Samuel C. Mozley Luis C, Garcia Under Contract with: American Electric Power Service Corporation Indiana and Michigan Electric Company Special Report No. 44 of the Great Lakes Research Division The University of Michigan Ann Arbor, Michigan March 1972 INTRODUCTION In Part VII (March 1971) of our report series relative to the Donald C. Cook Nuclear Station, we established the following report format: A. COOK PLANT PREOPERATIONAL STUDIES A.l Recording of Local Water Temperatures A. 2 Study of Floating Algae and Bacteria A. 3 Development of a Monitor for Phytoplankton A. 4 Study of Attached Algae A. 5 Study of Zooplankton A. 6 Study of Aquatic Macrophytes A. 7 Study of Benthic Organisms A. 8 Study of the Local Fishes A, 9 Support of Aerial Scanning B. SURVEYS OF EXISTING WARM WATER PLUMES C. THE ICE BARRIER AT THE COOK PLANT SITE D. EFFECTS OF EXISTING THERMAL DISCHARGES ON LOCAL ICE BARRIERS E. EFFECTS OF RADIOACTIVE WASTES IN THE AQUATIC ENVIRONMENT E.l Gamma Scan of Bottom Sediments E.2 The Most Sensitive Organism for Concentration of Radwastes E.3 Study of Lake Michigan's Present Radioactivity Content (FINISHED) This report covers only items A, 2, A. 5, and A. 7 of the above format. These studies constitute our initial survey of the large-scale set of biology stations related to the Donald C. Cook Plant and were carried out on 10 July 1970. The layout of sampling stations, with indication of how the stations are numbered is given in Figure 1. The sampling stations, their positions relative to the Cook Plant, tl;eir distances offshore, and the water depths encountered are given in Table 1. CO Q) •H s O OO —i I I u o I I u X.. \ CO a o u cd u en a •H o o o I ■^ o oo#~^ I u \ o o«— . I IT) Q O qOO#— '^. o o o ^ <=> o w ->o o oo»— ^ O 0«— rH 00«— CN o a o c ro o li o •H 4J fD CO Q) 4J Q) B O u oo«— ^ in I I o Q CO \ CO Q) •H ■H O O OO — [- I "I 'M" ' I 00 CN iH O CO 0) ^ ^ O 4J •H +J CO Cd -H CU 4J ^ CO fl H o U -H O ■!-» • iz; cd a C o •V txO •H 4J 'H 4J a CO cd CO 0) 4-J tH ^ CO P-. (U CU ri^ ^ rC O 4-J 4J o cj a M-l •H o T3 rH M u cd 0) (U a ^ rQ o e e Q :3 ^J a a ^X) Cd tH fl ■M o • CO U a o C CU CU O M CO CO •H -H •H 4J 'x:) «» CO cd iH ^ ^ CU a o CO rC O tH ■p 1 tH o a O Q M-l O M-l O -P CU AS CO rC ^ a cd 4-J +j cd :3 rH Xl XI o p. Q) a CO O 4-) cd +J Cd J-l >. a •^ O rC W) 4J a •H a rC CO cd U Q) Q) tH u ^ x> a. O 4J a CU Q) M-l u x^ CO o cd 4J CU rH J-l CO U-l •H (U a o 6 ^ o •H ^ M-l :3 4J 4J o c cd u 4-» O J-l rH CO a CU cd ^ -H CU CU 6 u rC J-l :3 CU H cd C CO TABLE 1. The Sampling Stations, Their Positions Relative to the Cook Plant, Their Distances Offshore, and the Water Depths Encountered on 10 July 1970. station Position Relative to the Cook Plant Water Depth (ft) DC-1 Directly off the plant, 1/4 ml offshore 19 DC- 2 It It It 3/4 •• It 40.5 DC-3 It It tt 1 1/4 " It 56.5 DC-4 It It It 2 1/4 " tt 65.5 DC-5 It It It 4 It 79.5 DC- 6 It It It 7 It 130.5 NDC-.25-1 1/4 ml north of the plant, 3/4 mi offshore 38 NDC-.5-1 1/2 " II It II It 1/4 It It 20.5 NDC-.5-2 II II It It It II 1/2 II It 26.5 NDC-.5-3 II II It It It " 1 1/4 II It 56.5 NDC-1-1 1 II It It It It 1/4 It It 18.5 NDC-1-2 It II It It It It 3/4 It It 33.5 NDC-1-3 II " It It It It 2 1/4 It It 57.5 NDC-2-1 2 II It It It It 1/4 " It 18.5 NDC-2-2 11 II It It It II 1/2 It II 21.5 NDC-2-3 II II It It It It 1 1/4 It It 51 NDC-2-4 II It It It It It 4 It It 74.5 NDC-4-1 4 It It It It It 1/4 It It 17.5 NDC-4-2 II II It It II It 1/2 It It 29 NDC-4-3 II It It It It It 2 1/4 It It 55.5 NDC-4-4 It II It It It It 7 It It 134.5 NDC-7-1 7 It It It It " 1/4 II It 22 NDC-7-2 II It tt It It II 1/2 It It 27.5 TABLE 1 continued Station Position Relative to the Cook Plant Water Depth (ft) NDC-7~3 NDC-7-4 NDC-7-5 SDC-.25-1 SDC~.5-1 SDC-.5-2 SDC-.5-3 SDC-1-1 SDC-1-2 SDC-1-3 SDC-2-1 SDC-2-2 SDC-2-3 SDC-2-4 SDC-4-1 SDC-4-2 SDC-4-3 SDC-4-4 SDC-7-1 SDC-7-2 SDC-7-3 SDC-7-4 SDC-7-5 7 mi north of plant, 1 1/4 mi offshore If 11 If n I! 2 1/4 " " II It II It 1/4 " south " " -j /o It 11 It It It It It It It It It II It It It II It It It It It It II It It It It It It II It It It II It It It II It It II It It It It It It It It It It It It It It It It It It It It II M It It It It It 3/4 " 1/4 " 1/2 " 1 1/4 " 1/4 " 3/4 " 2 1/4 " 1/4 " 1/2 " 1 1/4 " 4 1/4 " 1/2 " 2 1/4 " 7 1/4 " 1/2 " 1 1/4 " 2 1/4 " 4 It II It It 48 52,5 71.5 49.5 19.5 28.5 54.5 13.5 40 61.5 18 27.5 51.5 72.5 14 37.5 59.5 102.5 14 26.5 51.5 53.5 70.5 TABLE 1 continued. Additional Stations for Phytoplankton Only. (All in 4 Ft of Water) station Position Relative to the Cook Plant NDC-.5-0 1/2 mi north of the plant, just off the beach NDC-1-0 1 II II II II II II II II II NDC-2-0 2 II II II II II II 11 II II NDC-4-0 4 II II 11 11 11 11 II 11 11 SDC-.5-0 1/2 " south " " " " " " " SDC-1-0 1 II 11 11 11 II 11 11 11 II SDC-2-0 2 II 11 11 11 II 11 11 11 11 SDC-4-0 4 II II 11 II II II 11 II 11 Phytoplankton samples were taken at all the stations of Table 1. At all stations with serial numbers greater than zero, zooplankton, benthos, and physical measurements were collected as well. Total collections were: 53 phytoplankton samples and 46 each of zooplankton, benthos, and the physical measurements. The physical measurements consisted of surface water tempera- ture, water depth, bottom types, Secchi disc water transparency, and water color as seen above the white 30-cm Secchi disc. Weather conditions and wind and wave characteristics were taken and meteorological data taken on 10 July 1970 apply to all the sections of this report; these data are presented in Appendix A. A. COOK PLMT PREOPERA.TIONAL STUDIES A. 2 Study of Floating Algae and Bacteria Techniques for bacteria had not been mastered at the time of this survey. Phytoplankton Techniques Phytoplankton samples were collected by Hansen bottle at a depth of 1 m, with the exception of the nearshore stations. Nearshore collection (serial number zero stations) were made by submerging an open 1-liter bottle 4 in. below the water surface. All samples were 1-liter whole samples. Each sample was fixed with Utermohl's iodine fixative immediately after collection and stored in an opaque container. In the laboratory, each sample was concentrated to 100 ml by settling in a 1000-ml graduate cylinder and siphoning off 900 ml of fluid. The concentrated sample was stored in a 100-ml opaque bottle. Samples were prepared for counting by placing an aliquot of the concen- trated sample in a tubular combination settling and counting chamber and allow- ing the aliquot to settle overnight. The counting chamber containing the settled cells was then separated from the settling chamber, covered, and placed on the microscope. The samples were counted on an inverted binocular microscope at lOOOx magnification. Solitary species, green and blue-green algae colonies, and the filaments of filamentous forms were each counted as one cell. Each colonial diatom cell was counted except when the size of the filaments or colonies prohibited counting the individual cells; in this case, the number of individual cells was estimated. Phytoplankton Summary The phytoplankton summary which follows (Table 2) is based on the one used by the Michigan Water Resources Commission in reporting their phytoplankton collections. Our summaries differ only in that we have counted or estimated the cells in filamentous and colonial diatoms, while the Commission counts a filament or colony as a single organism. The station-by-station phytoplankton records constitute Appendix B. Dominant and Codominant Phytoplankters In each phytoplankton sample, one species or group typically was present in substantially greater numbers. We have called these species or groups "dominant." In six of the stations, however, a second species or group chal- lenged the numerical superiority of the dominant species. When the challenging species or group closely approached the cell numbers of the dominant species, the second most abundant species or group was recorded as a "codominant," and both are listed in the dominant species column of Table 2. In Table 3, those species or groups which were numerically dominant in the samples of the 10 July 1970 Cook Plant survey are presented. In the 53 phytoplankton samples of the survey, there were 59 dominant or codominant species or groups, of which 49 were diatoms. On the basis of the dominants and codominants of this survey. Lake Michigan in the region of the Donald C. Cook Plant was definitely a "diatom lake" in early July 1970* Qi U Q +J O Q U 4-1 cd J-l H 0) o C/2 0) •H o :3 PQ H td a •H e o Q CO CO O iH H < U Q) (1) cd 4-> H o <: en Tl •H e CO (U Q Q) CO ■P CO o C +J C CO Q) •H Pm Q a CO •H u u ■P c CO 0) •H u Q 0) CO W) -i }-i Q) Q) ^ w CO •H 4J TO CO C M (U ■U C CO o (1) ■u c o (IJ }-l MH o CO to •H •H J-l }-i CO CO r-{ iH tH •H (1) M ^ CO CO VI H tM C30 00 cx) vO 00 0) CO TO cn 4J •H •H CO TO CO U ^ U a CO d) TO (U ^J a (U c 4J o a o TO 4J Q) 4J Q) O U-l O C u M Q) o CO ^ M-l M CO CO ' ■ VD CM O 00 - a a 0) Q) Q) M-l M-l m TO c 43 0) f W 0) CO CO CO CO CO a CO CO J-l 4J ^ 4J 4J U 0) 4J

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CM o m CM o> 00 in vO in r^ C3> r^ o vD O CM in CM CO 00 in o d- ^ m VO 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 u CJ Q_) u C_) CJ o u U CJ U O o U U u u U CJ u O CJ u QJ u U p Q p p P p p Q P p P Q P p P P p P p Q Q p p p P ^ 53 'iZi » 525 !z; ;zi z ^ IS IS ^ ^ » z 125 iz; ;z; 53 13 53 53 Q) < H CO (U •H o C/D c •H e o Q CTJ Cd •U GO O rH Q) cd O <1 (U Q (U W ■M 6 Cd o a 4J c Cd 0) •H eiH P a cn •H B j-i o 4-J ■u c cd dJ •H u Q 0) CO 60 (U Cd 4J iH cd P^ ,H CO :3 1 o c cd 4J Q) rH a Q) •H CU )-i f^ e o o a O }-i 0) I 3 QJ PQ cd CO •H O 13 •H O I C U 0) 0) O 13 0) O «H }-( CJ pq O I cd CU u u a. 4J C30 rH • CN CO cd cdt 4J A. cti ct »-l U 4-t +J CO cr Q) • a C a c ^ CO o r^ -vT rH CO ^ vO O O CM CSJ CT\ O CJ> CO CM 00 -< 4-J Cd c iH (U iH ^d CD •H JD C Cd P H C3^ <» •. o o VO o CX5 VO <7^ VO VO VO CO a\ o C30 CO CO CM CO CO tH rH CM -sf r^ vt C3^ CJ> CO O rH rH CO CO CM Cvj O bO o VO d- CO O r^ m iH rH VO tH iH VO CN O C30 vO CvJ CM o 00 C>4 00 00 iH CO vd- o CO CM VO CO LO Cvl CO CO rH o CO VO CM vO vO 00 o :zi CM I O in I CM in 00 in C3^ <3^ in m CO CO O O CM o o o CM I CO I in I Q 53 I I I CJ C-3 CJ Q Q Q CO c/3 en I Q CO 00 ON o 00 o • Q rH o "z; o CM CM CM CM Cy\ O CN rH o (7N jz; o CM CNj rH CN 00 vo in CJN rH rH CN o a\ CM ,H I I CO CO CO I I I CD Q CO CO -^ I I CM CM I I CD CD O I -^ I CD Q CO I St I CD Q I I CD Q CO CO I I CD Q CO 11 TABLE 3. Dominants and Codominants in the Samples of the Survey Species or Group Dominant or Codominant Occurrences Tabellaria fenestrata (diatom) Cyclotella sp. (diatom) Melosira spp. (diatoms) Fragilaria crotonensis (diatom) Unidentified green algae Unidentified blue- green algae Dinobrvon diver^ens (flagellate) Aphanothece sp. (blue-green) Hypnodinium (?) sp. (dinof lagellate) Microcystis aerup;inosa (blue-green) Chlamydomonas sp. (flagellate) 32 7 6 4 4 1 1 1 1 1 1 TOTAL 59 Spatial Distribution of Dominants and Codominants Little can safely be said about spatial distribution of phytoplankters, for they can be exchanged from water mass to water mass by turbulent mixing. The problem is compounded by the annual, seasonal, preoperational, and post- operational differences which may be encountered in a continuing series of surveys, such as required for the Cook Plant. Although we are, at present, only beginning to be accustomed to having massive phytoplankton data with which to work, we have noted some character- istics of the spatial distribution of dominant and codominant phytoplankton species which appear to be worthy of record. 12 In this survey, the dominant organisms in the surf -zone stations (serial number zero) were the diatom groups Melosira sp. and Cyclotella sp. In the rest of the area, the diatom Tabellaria fenestrata was the most frequent domi- nant. In stations farthest offshore, the diatom Fragilaria crotonensis was more apt to be dominant than in the inshore stations. The Master List of Phytoplankters Collected During the Survey Another of the requirements in a long-term series of surveys aimed at de- tecting changes in phytoplankton populations over a period of time is the rou- tine presentation of lists of all the phytoplankters collected during each sur- vey. Over a period of years, such "master lists" become the means of detecting the arrival of new species or the vanishing of species originally present. We do not expect the latter to happen; our present knowledge of the Lake Michigan phytoplankton indicates that species are not eliminated from the phytoplankton population, but rather that new species appear and are added to the population. The master list of phytoplankters collected during the survey of 10 July 1970 is presented in Table 4. In this table there are incomplete identifica- tions and unidentified organisms , generally occurring in the green or blue- green groups. We make no apologies for this. Our primary attention is di- rected to the diatom groups where population composition change is apt to show soonest. Our collections are preserved, and may be re-studied if other organ- isms or groups exhibit changes which indicate the desirability of re-study. For convenience in inspection of names, the contents of Table 4 are arranged alphabetically . TABLE 4. Master List of Phytoplankton Collected on 10 July 1970. 13 Achananthes hauckiana Achnanthes sp . Amphipleura pellucida Amphiprora ornata Amphora ovalis Amphora ovalis v. pediculus Amphora sp. Anabaena circinalis Anabaena sp. Ankistrodesmus braunii Ankistrodesmus falcatus Aphanothece sp. Asterionella formosa Blue-Green unknown colonies Ca lone is sp. Caloneis ventricosa Caloneis ventricosa v. truncata Ceratium hirundinella Chlamydomonas sp. Chlorella sp. Chroococcus limneticus Chroococcus turgidus Chroococcus sp . Closterium sp. Closteriopsis longissima (continues on right column) Coelastrum sp . Coelastrum sphaericum Coelosphaerium sp. Cosmarium sp. Crucigenia quadrata Crucigenia sp. Cryptomonas sp. Cyclotella meneghiniana Cyclotella sp. Cymatopleura solea Cymatopleura solea v. apiculata Cymbella sp. Dactylococcopsis sp. Diatoma tenuis v. elongatum Diatoma vulgare Dictyosphaerium pulchellum Dinobryon divergens Dinof lagellate cysts Diploneis sp. Flagellates Fragilaria brevistrata Fragilaria capucina Fragilaria construens Fragilaria crotonensis Fragilaria intermidia Fragilaria leptostauron TABLE 4 continued 14 Fragilaria pinnata Franceia droescheri Franceia ovalis Franceia sp. Glenodinium sp. Gloeocvstis sp. Golenkinia radiata Gomphonema sp. Green cells Green cells, little Green cells, round, unknown Green cells, tiny Grenn colony, unknown Greens , unknown , chains Greens, unknown, grape like Hvpnodinium sp. Kirchneriella sp. Lagerheimia citriformis Lagerheimia longiseta Lagerheimia longiseta v. major Lagerheimia sp. Mallomonas sp . Melosira binderana Melosira granulata Melosira granulata v. angustissima Melosira islandica Melosira italica Melosira sp. Melosira varians Meridion circulare Microcystis aeruginosa Microspora sp. Mougeotia sp. Navicula capitata Navicula costulata Navicula decussis Navicula gastrum Navicula sp. Navicula tripunctata Neidium dubium Nephrocytium sp. Nitzschia acicularis Nitzschia sp. Oocystis borgei Oocystis solitaria Oocystis sp. Oocystis submarina Oscillatoria sp. Fed last rum duplex Pediastrum simplex Pediastrum sp. Peridinium sp. Phormidium sp. Quadrigula chodatii (continues in right column) TABLE 4 continued 15 Quadrigula lacustris Quadrigula sp. Round cells, broken colonies Scenedesmus abundans Scenedesmus acuminatus Scenedesmus armatus Scenedesmus bijuga Scenedesmus bijuga v. alternans Scenedesmus dimorphus Scenedesmus incrassatulus Scenedesmus opoliensis Scenedesmus quadricauda Scenedesmus quadricauda v. maximus Scenedesmus sp. Schroederia judayi Sorastrum spinulosa Spores Spores, resting Stephanodiscus sp. Staurastrum sp, Stauroneis sp. Surirella angustata Surirella sp. Synedra acus Synedra delicatissima Synedra delicatissima v. angustissima Synedra filiformis (continues in right column) Synedra ostenfeldii Synedra sp. Synedra ulna Synedra ulna v. chaseana S ynedra ulna v. danica Synedra vaucheriae v. fragilarioides Tabellaria fenestrata Tetraedron lunula Tetraedron minimum Tetraedron obesum Tetraedron pentaedricum Tetraedron regulare Tetradesmus smith ii Tetradesmus wisconsinensis Tetrastrum sp. Treubaria setigerum Tribonema sp. Unknown cells Unknown colonies Westella sp. Zoospores 16 Diversity Indices of the July 1970, and Earlier, Phytoplankton Collections In this section we follow Wilhm and Dorris (1968), who developed from information theory a technique for evaluating the structure of bottom fauna communities. We have applied their technique to our phytoplankton collections because (1) the technique is considered to be very largely independent of sample size (allowing the use of our smaller, earlier collections); (2) the technique mathematically considers each component of the population collected; and, most important, (3) the technique is an accepted index of community struc- ture by which to watch for changes in the phytoplankton community structure around the Cook Plant in the ensuing years. Basically, the Wilhm and Dorris diversity index considers that, in a popu- lation composed of a few species or groups and with large numbers of individuals of each species or group, the uncertainty that any one organism collected will belong to a species or group already taken will be low (and the technique com- putes a low diversity index). Conversely, in a community composed of many species or groups, but with fewer numbers of individuals of any species or group, the uncertainty that any particular organism collected will belong to a pre- viously recognized species or group is hi^h (and the technique computes a high diversity index) . The computation of Wilhm and Dorris is d = -^(N^/N) log^ (N^/N) in which (N./N) is the percentage of the population, N, that is represented by any one species or group, N., of the collection. Logarithms to the base 2 are natural logarithms multiplied by 1.44269; the logarithms are negative, and a *Wilhm, J.L.. , and Dorris, T.C. 1968. "Biological Parameters for Water Quality Criteria." BioScience 18(6) :477-81. 17 negative summation is used to provide an answer in positive numbers. The over- bar on d denotes a mean, for their method (extended) also yields maximum and minimum diversity indices. NOTE: The average diversity index shown at the end of Table 6 is merely the arithmetical average of d values of the individual station collections. In accordance with our policy of continued analysis of our earlier surveys of the Cook Plant area, we have computed the Wilhm and Dorris diversity indices of our phytoplankton collections earlier than July 1970 (the collection on 25 April 1969 was not made by a comparable method and is not included). The station lists of phytoplankton collected from which these diversity indices have been computed are Tables 3, 4, 5, and 6 of Part VII of our report series rela- tive to the Cook Plant. The results are given in Table 5. TABLE 5. Diversity Indices of Phytoplankton Samples 1-1.3 Miles Off Cook Plant Station and Date Distance from Shore Depth of Collection Diversity Index, d CP-2 11 August 1969 COOK 4 October 1969 COOK 26 April 1970 COOK 6 June 1970 1.3 miles 1#0 mile 1,0 mile 1.0 mile 6 inches 15 meters 15 meters 15 meters (and, for comparison, from the present report) DC- 3 10 July 1970 1-" "'^^^^ ^ '"^'^^^ 3.64 2.38 3.11 3.27 3.60 18 TABLE 6. Numbers of Phytoplankton Species, Number of Individuals Per Milliliter and Diversity of the 10 July 1970 Survey station Number of Number of Diversity Species Individuals Index DC-1 23 390 3.18 DC- 2 24 407 3.63 DC- 3 30 647 3.60 DC-4 15 448 1.85 DC- 5 12 286 2.88 DC- 6 11 333 2.39 NDC-.25-1 26 458 3.39 NDC- . 5-0 42 1,794 3.92 NDC-.5-1 43 504 3.88 NDC-.5-2 26 647 3.53 NDC-.5-3 17 528 2.89 NDC- 1-0 36 3,052 3.90 NDC- 1-1 31 1,244 3.88 NDC- 1-2 20 974 1.65 NDC- 1-3 25 543 3.28 NDC- 2-0 36 1,180 4.27 NDC- 2-1 43 940 4.05 NDC- 2- 2 21 504 3.85 NDC- 2- 3 21 601 3.46 NDC- 2- 4 20 515 2.91 NDC- 4-0 30 1,856 4.01 NDC-4-1 46 3,024 3.89 NDC-4-2 42 1,521 4.01 NDC-4-3 19 277 2.85 NDC- 4- 4 19 331 3.40 NDC- 7-1 46 1,594 3.78 NDC- 7- 2 50 11,523 1.09 NDC-7-3 41 1,081 3.92 NDC-7-4 17 1,344 2.77 NDC-7-5 21 220 3.29 TABLE 6 continued 19 Station Number of Species Number of Individuals Diversity Index SDC-.25-1 9 143 1.94 SDG-.5-0 40 1,038 3.90 SDC-.5-1 33 718 3.62 SDC-.5-2 17 278 2.84 SDC-1-0 33 1,396 3.81 SDC-1-1 27 830 3.33 SDC-1-2 19 757 2.03 SDC-1-3 16 386 2.75 SDC-2-0 34 962 3.55 SDC-2-1 29 1,012 3.58 SDC-2-2 13 265 2.55 SDC-2-3 18 337 3.23 SDC-2-4 12 316 2.29 SDC-4-0 40 1,104 4.22 SDC-4-1 31 567 3.56 SDC-4-2 26 798 3.08 SDC-4-3 18 438 2.84 SDC-4-4 17 331 3.15 SDC-7-1 32 632 3.68 SDC-7-2 42 13,274 0.49 SDC-7-3 37 630 3.54 SDC-7-4 26 712 3.20 SDC-7-5 23 413 2.88 Overall Average : Diversity Index 3.20 20 In Table 6, the number of species or groups present, the number of indi- vidual cells per milliliter, and the diversity index for each phytoplankton sample of the 10 July 1970 survey are listed by stations. The field of diver- sity indices has been contoured and is shown in Figure 2. Comments on the Phytoplankton Collections Consideration of the numbers of species collected, the nimibers of individ- ual phy toplankters , and of the diversity indices of the station collections (horizontally across Table 6) show only that the individual station collections were greatly different from each other. The adjacent stations, SDC-7-1, SDC-7-2, and SDC-7-3, for example, show variations in numbers of species or groups rang- ing from 32 to 42, variations of numbers of individuals per milliliter from 630 to 13,274, and variations of the diversity index from 0.49 (at SDC-7-2) to 3.68 (at SDC-7-1). The conclusion being forced upon us is that small water masses, each with different biotic characteristics, move through the Cook Plant area. The data from our grab-sampling technique is the manifestation of uneven phytoplankton population distribution in these water masses. We have seen, but perhaps not fully appreciated, similar conditions before (see our conclusion in Part VII of our report series wherein floating-bag experiments at NIPSCO's Bailly Station produced only evidence of plankton patchiness). There are many other evidences of plankton patchiness shown in Table 6. The demonstrable phytoplankton patchiness shown in Table 6 leads us to the conclusion that the overall average diversity index for this survey should be presented only as an objective mathematical summary of data from several 21 w u ;3 o u c o u CO o •H 0} > a o PL. o u O M 22 biologically different water masses. This figure will be retained for pos- sible future usefulness. Possible Influence of the St. Joseph River Dr. E. F. Stoermer has provided a list of river-associated phytoplankters which he believes would, if heavily dominant in our Cook Plant surveys, in- dicate an undesirable amount of influence by the St. Joseph River on the en- virons of the Cook Plant. Our phytoplankton collections at Cook Plant on 10 July 1970 have been inspected for the presence and degree of numerical dominance of these species. In no station did all 13 of the species occur. In 16 of the 54 stations none of the proscribed species occurred. When numbers of these species are plotted on the map of Cook Plant sampling stations (Figure 3), the only pattern that emerges is of their more frequent occurrence in shallow water, not a surprising result since these species also are known as shallow-water lake plankters. Thus, the evidence from the 10 July 1970 sur- vey shows no demonstrable effect of the St. Joseph River on the Cook Plant plankton. Similar analysis will be made for each of the subsequent surveys. 23 ^ 4J bO 0) C rC •H 4J ^ O TJ rC C en cd CO a a o O 4J •H ^ •u C • CO cd c 4J rH CO CX-H O 4-» W) 4J cd a >> 4J •H 4:: en rH fL, a ^ e ^ cd QJ Cd Oi u w cd 4J -H +J c Cd Cd tH CO u CL, CO C < 0) ^ 1 CO J-l CU Q) u u > (^ •H M-< pc:; CO QJ ^ •H a. a cd OJ is^ a p. en •H • 4J M-l CO :3 rO s-d ^ u rn a CO H -H 3 LU o S8 — b- t m W ^ S c 4J OJ Cd C/3 • u (U 4-» 'H >^ c -d p a' c CO •H 4-J QJ ro CM ro J-i CO 000— ^ c a -H a c ^ QJ 4-J •u > - ^10 Jt^ ^ ^ 00 CT3 P QJ cd CO — ^* .^ rH tH ^ P- p. • u 4-J 4J CO QJ > ^ >-. QJ ^ a. a u 13 OCX)- "^ TH QJ a x) p. o"?)S- - CU Cd 0) CO 4J 4-» P Cd M-< QJ •H •H a u Cd M -ri m (p CO CO QJ 'Td •00- CM CO cd CO X» Cd Cd g Id QJ j-i V4 P ^ QJ QJ 4-i > > QJ •H •H ^ >, P^ (^. ^ r^ E o s • • 000— ^ CVJ ^ CNJ »0 o o • 00 — CO UJ 1 I I "I "I'^'l r<. ^ fo CM — o 24 A. 5 Study of Zooplankton Zooplankton Techniques Zooplankton collections were made by a vertical haul, from bottom to sur- face, with a #5-mesh (0.282-mm average openings) net of .5-m diameter. A pro- peller-type flowmeter was affixed in the center of the net mouth to obtain quantitative measurement of the volume of water sampled by the net. The volume of water that passed through the net was indicated by the number of revolutions made by the flowmeter propeller; this figure was recorded and later converted to an equivalent expressed in liters of water. The net was then raised above the surface and rinsed to free organisms impacted on the net and to concentrate the sample in the collecting jar tied on the narrow cod-end of the net. Then, excess water in the brim-full jar was decanted through a small area of the net just above the cod-end. This small area of the net was then rinsed carefully to wash all zooplankters into the collecting jar with a minimum amount of water. The jar was removed from the net, and Koechies fixative, a solution of formalin and sugar, was added as a preservative. An identification label containing pertinent collection data was placed in the jar. The jar was capped and labeled exteriorly for delivery to the laboratory. In the laboratory, the sample volume was measured by transferring the entire sample to a graduated cylinder. The entire sample then was returned to the collecting jar and mixed thoroughly and continuously with a magnetic stirrer while 1-ml subsamples were extracted with a Henson-Stempel pipette. Each sub- sample was placed in a depression in a clear glass spot plate. Each depression 25 received a few drops of soap solution to break the surface tension film and allow the zooplankton to settle to the bottom for easier counting. A variable- magnification binocular microscope was used, with transmitted light, for counting and identification. As many 1-ml subsamples as were necessary to ob- tain good statistical parameters were counted. The number of zooplankton per liter of water was obtained by conversion with standard factors. The station collections of zooplankton on 10 July 1970 are given in Table 7. Zooplankton Abundances The Cyclopoid copepod zooplankton group exhibited the highest abundances during this survey, reaching 21.99 individuals per liter at station SDC-4-4 , 13.52 individuals per liter at station DC-6, and 13.44 individuals per liter at station NDC-4-4; these copepods , however, were not present in the collection from station SDC-4-3. Other maximum abundances during this survey were: Diaptomus copepods, with 12.66 individuals per liter at station SDC-4-4 (this was the highest abundance for this group, greatly exceeding its abundances at other stations); Bosmina cladocerans, with 12.62 individuals per liter at sta- tion SDC-2-1 (a single-station maximum approached only by 9.60 individuals per liter at station SDC-7-3) ; Polyphemus cladocerans, with 2.35 individuals per liter at station DC-1 (at all other stations this group had abundances of less than 1 individual per liter); and Asplanchna rotifers, with 1.42 organisms per liter at station SDC-2-1 (abundances from other stations closely approached this figure). Actual numerical abundances of zooplankton in the collections of this survey indicate primarily the patchiness in spatial distribution of the zooplankton. 26 In seeking to establish a biological baseline against which future com- parisons may be made, we list below the dominance frequencies of the zooplank- ton groups of the 10 July 1970 survey. Although the Cyclopoid copepods were occasionally present in higher numbers, Bosmina cladocerans dominated the samples most frequently. Zooplankton Group Cyclopoid copepods Diaptomus copepods Bosmina cladocerans Polyphemus cladocerans Asplanchna rotifers Dominant or Codominant Occurrences 8 37 2 Just as the phytoplankton samples in the preceding section of this report required that the Cook Plant area of Lake Michigan be put on record as being a '*diatom" lake on 10 July 1970, the zooplankton collections of the same day require that the Cook Plant region of the lake be recorded as a "Bosmina" lake. Diversity Indices of the July 1970, and Earlier ^ Zooplankton Collections In the preceding section on phytoplankton, we have introduced and discussed the Wilhm and Dorris computation of the diversity index. In this section, their index is applied to the zooplankton collections because it appears to be, at present, the best means by which the zooplankton community can be represented objectively in a way that can be used in watching for changes in community composition over time. We, at this time at least, attach no pollution-related interpretations to the numbers for diversity indices that are computed. They are used merely as objective parameters against which comparisons over time are to be made. 27 TABLE 7. Zooplankton, 10 July 1970. Samples by Vertical Haul of Metered #5 Net. Organisms Per Liter. ^ Stations DC-1 DC-2 DC-3 DC-4 DC-5 DC-6 Organisms ^ Copepods : Diaptomus 0.26 0.17 0.26 0.49 1.54 5.02 Epischura - - 0.01 0.01 0.01 Eurytemora .----- Limnocalanus - - - - - 0.02 Senecella .----- Cyclopoids 0.05 0.38 0.11 0.79 3.96 13.52 Harpactacoids - 0.01 - - - Cladocerans : Alona - 0.02 - Bosmina 0.56 1.69 2.49 Ceriodaphnia - 0.04 Daphnia - 0.02 0.02 Diaphanosoma ------ Eurycercus ------ Holopedium - 0.01 0.02 0.03 Leptodera - 0.04 0.01 Polyphemus 2.35 0.47 0.11 0.14 0.25 0.39 Rotifers : Asplanchna 0.42 0.34 0.21 0.14 0.04 0.05 3.27 1.86 4.61 0.03 0.02 0.02 0.21 0.17 0.13 28 TABLE 7 continued "- ^Stations NDC.25-1 NDC-.5-1 NDC-.5-2 NDC-.5-3 NDC-1-1 Organisms \ \ ^ Copepods : Diaptomus 0,30 0.16 0.22 0.41 0.13 Epischura - - - - » Eurytemora - - - - « Limnocalanus - - - - _ Senecella - - - « . Cyclopoids 0.34 0.06 0.24 0.24 0.18 Harpactacoids - - - 0.01 Cladocerans : Alona - 0.06 0.02 - 0.03 Bosmina 3.36 1.07 2.20 3.22 1.50 Ceriodaphnia 0.04 - 0.04 0.02 0.03 Daphnia 0.02 - - 0.04 0.03 Diaphanosoma - - - - - Eurycercus - - - - - Holopedium - - - 0.01 - Leptodera - - - 0.01 - Polyphemus 0.98 0.14 0.82 0.25 0.41 Rotifers : Asplanchna 0.68 0.53 0.98 0.23 0.52 29 TABLE 7 continued \ Stations NDC-1-2 NDC-1-3 NDC-2-1 NDC-2-2 NDC-2-3 Organisms^ Copepods : Diaptomus 0.27 0.41 2.02 - 0.26 Epischura - - 0.02 Eurytemora - - - . . Limnocalanus - - - - - Senecella - - - _ - Cyclopoids 0.24 0.32 4.43 - 0.24 Harpactacoids - - - _ « Cladocerans : 2.31 0.01 0.04 Alona 0.01 0.36 - Bosmina 1.58 1.17 4.72 Ceriodaphnia - - 0.03 Daphnia 0.02 0.01 0.35 Diaphanosoma - - - Eurycercus - - - Holopedium 0.01 - 0.01 Leptodera - - 0.01 Polyphemus 0.58 0.36 0.06 Asplanchna 0.42 0.04 0.13 0.02 0.01 0.31 0.32 30 TABLE 7 continued ^^ Stations NDC-2-4 NDC-4-1 NDC-4-2 NDC-4-3 NDC-4-4 Organisms -^ Copepods : Diaptomus 2.02 0.27 0.21 0.51 6.60 Epischura 0.02 0.02 0.02 0.01 0.01 Eurytemora - - - - - Limnocalanus - - - - 0.03 Senecella - - - - - Cyclopoids 4.43 0.07 0.17 0.70 13.44 Harpactacoids - - - 0.01 - Cladocerans : Alona - - 0.01 - - Bosmina 4.72 1.96 1.58 1.59 3.77 Ceriodaphnia 0.03 0.02 0.02 0.07 0.02 Daphnia 0.35 0.02 0.01 0.08 0.23 Diaphanosoma - - 1.58 - - Eurycercus - - 0.02 - - Holopedium 0.01 0.02 0.02 0.02 0.01 Leptodera 0.01 - 0.01 0.01 - Po lyphemus 0.06 0.50 0.11 0.25 0.13 Rotifers : Asplanchna 0.13 0.38 0.25 0.24 0.05 31 TABLE 7 continued ^ stations NDC-7-1 NDC-7-2 NDC-7-3 NDC-7-4 NDC-7-5 Organisms" v^ Copepods : Diaptomus 0.66 1.13 0.81 0.44 0.99 Epischura - - - 0.04 0.03 Eurytemora - - - - - Limnocalanus - - - - - Senecella - - - - - Cyclopoids 1.33 2.02 0.93 0.55 3.19 Harpactacoids - - - 0.01 - Cladocerans : Alona 0.08 0.42 0.14 0.01 - Bosmina 5.72 4.22 1.62 1.11 1.60 Ceriodaphnia - 0.07 - 0.04 0.03 Daphnia 0.05 0.13 0.05 0.04 0.14 Diaphanosoma - - - - - Eurycercus 0.05 - - 0.01 0.01 Holopedium - 0.05 0.01 0.01 0.01 Leptodera - - 0.03 0.01 0.01 Polyphemus 0.23 0.15 0.47 0.16 0.10 Rotifers : Asplanchna 0.20 0.37 0.29 0.10 0.03 32 TABLE 7 continued ^ ^tations SDC-.25-1 SDC-.5-1 SDC-.5-2 SDC-.5-3 SDC-1-1 Organisms^ Alona 0.04 Bosmina 4.31 Ceriodaphnia 0.02 Daphnia 0.08 Diaphanosoma - Eurycercus - Holopedium 0.03 Leptodera - Polyphemus 0.69 Rotifers : Asplanchna 1.08 0.02 Copepods: Diap tonus 0.37 0.25 0.08 0.60 0.08 Epischura - - - - « Eurytemora - - » . _ Limnocalanus - - - - . Senecella - - - « _ Cyclopoids 0.48 0.23 0.23 0.38 0.25 Harpactacoids - - - - - Cladocerans : 4.88 0.02 - 0.06 2.25 3.53 3.23 - 0.07 - - 0.15 « 0.03 - 0.01 0.03 0.21 0.45 0.02 0.18 0.66 0.44 1.26 0.36 0.48 33 TABLE 7 continued "^ ^tations SDC-1-2 SDC-1-3 SDC-2-1 SDC-2-2 SDC-2-3 Organisms ^ Copepods : Diaptomus 0.34 0.68 1.78 0.08 0.35 Epischura 0.05 0.02 0.24 0.04 - Eurytemora - - - - - Limnocalanus - - - - - Senecella - - - - - Cyclopoids 0.44 0.66 3.44 0.10 0.26 Harpactacoids - 0.01 - 0.04 0.01 Cladocerans : Alona 0.08 0.42 0.18 0.02 0.01 Bosmina 3.93 3.50 12.62 2.24 1.65 Ceriodaphnia 0.03 0.06 0.06 - 0.05 Daphnia 0.03 0.12 0.06 - 0.03 Diaphanosoma - - - - - Eurycercus - - - - - Holopedium 0.09 0.04 - 0.02 0.04 Leptodera - 0.04 0.12 - 0.05 Polyphemus 0.44 0.12 0.47 0.38 0.25 Rotifers: Asplanchna 1.20 0.32 1.42 0.50 0.22 34 TABLE 7 continued ^ Stations SDC-2-4 SDC-4-1 SDC-4-2 SDC-4-3 SDC-4-4 Organisms \ Copepods : Diaptomus 1.68 0.33 0.08 0.40 12.66 Epischura 0.01 - 0.05 0.04 - Eurytemora - - - - - Limnocalanus - - - - 0.09 Senecella - - - - - Cyclopoids 2.59 0.08 0.16 - 21.99 Harpactacoids - - - 0.04 - Cladocerans : Alona - - 0.06 - - Bosmina 4.75 1.69 1.08 2.74 9.83 Ceriodaphnia - - - - - Daphnia 0.19 0.03 - 0.12 0.35 Diaphanosoma - - - - - Eurycercus - - - - - Holopedium - 0.02 - - - Leptodera 0.01 - 0.02 0.03 - Polyphemus 0.23 0.14 0.35 0.08 0.18 Rotifers : Asplanchna 0.08 0.56 0.32 0.25 0.08 35 TABLE 7 continued ^^ ^ \S tat ions SDC-7-1 SDC-7-2 SDC-7-3 SDC-7-5 **v^ Organisms "^ '>>^ - ^ Copepods : Diaptomus 0.31 1.05 6.20 1.34 Epischura 0.04 - 0.19 0.03 Eurytemora - - - - Limnocalanus - - - - Senecella - - - - Cyclopoids 0.61 2.73 6.86 2.78 Harpactacoids - - - 0.02 Cladocerans ; Alona 0.19 0.46 0.20 0.03 Bosmina 3.51 3.97 9.60 1.05 Ceriodaphnia 0.04 - 0.05 - Daphnia - - 0.79 0.14 Diaphanosoma - - - - Eurycercus - 0.03 0.23 - Holopedium - - 0.05 0.02 Leptodera - 0.06 0.06 0.01 Polyphemus 3.66 0.99 0.64 0.12 Rotifers : Asplanchna 0.15 0.78 0.88 0.07 36 The diversity indices have been computed from the station collections of 10 July 1970 given in Table 7. Table 8 gives indices computed from earlier collections • TABLE 8. Diversity Indices of Zooplankton Samples from 1-1.3 Miles Off Cook Plant. Station and Date Distance from Shore Diversity Index COOK 1 A .1 on / r> *. 1. m^n 1-0 mile 2.0 4 October 1969 COOK T A -1 10 o^ A -1 TA-7A -^'O mile l.z 26 April 1970 COOK 1 A •-! 1 / r T TO-7A 1-0 mile 1.4 6 June 1970 (and, for comparison, from the present report) DC-3 10 July 1970 ^-25 "'^^^^ ^-^ Table 9 presents station by station: (1) the dominant organism in terms of numbers in each station collection, (2) the total number of zpoplankton organisms per liter captured by the #5 plankton net, and (3) the diversity in- dices as computed from the data of Table 7, At the end of Table 9 there is presented the overall average diversity index for this day's collections, which is given as a possible summary parameter for the station collections, Conaments on the Zooplankton Collections The numbers of zooplankton per liter found in the water in the Cook Plant area varied considerably. The offshore samples tended to exhibit higher abun^ dances; however, some stations closer to shore produced samples with high abun- dances of zooplankton. Table 9 lists, by station, the total number of organisms 37 TABLE 9. The Numerically Dominant Zooplankters, Total Numbers of Zooplankters Per Liter (Metered #5 Net), and Diversity Indices of the 10 July 1970 Collections, Station Dominant Organisms Total Organisms/Liter Diversity Index DC-1 DC-2 DC-3 DC-4 DC-5 DC-6 NDC-.25-1 NDC-.5-1 NDC-.5-2 NDC-.5-3 NDC-1-1 NDC-1-2 NDC-1-3 NDC-2-1 NDC-2-2 NDC-2-3 NDC-2-4 NDC-4-1 NDC-4-2 NDC-4-3 NDC-4-4 NDC-7-1 NDC-7-2 NDC-7-3 NDC-7-4 Polyphemus 3.64 Bosmina 3.19 Bosmina 3.24 Bosmina 5.11 Cyclopoids 7.85 Cyclopoids 23.76 Bosmina 5.72 Bosmina 2.02 Bosmina 4.52 Bosmina 4.44 Bosmina 2.83 Bosmina 3.13 Bosmina 2.67 Bosmina & Cyclopoids 11.78 missing Bosmina 3.52 Bosmina & Cyclopoids 11.78 Bosmina 3.26 Bosmina 2.41 Bosmina 3.13 Cyclopoids 24.29 Bosmina 8.32 Bosmina 8.19 Bosmina 4.35 Bosmina 2.53 1.5 2.1 1.3 1.7 1.8 1.6 1.8 1.8 2.0 1.3 2.0 2.0 2.2 1.8 1.7 1.8 1.8 1.8 2.4 1.6 1.5 2.6 2.4 2.3 38 TABLE 9 continued Station Dominant Org; anisms Total Organisms /Liter Diversity Index NDC-7-5 Cyclopoids 6.14 1.8 SDC-.25-1 Bosmina 7.10 1.8 SDC-.5-1 Bosmina 6.04 1.1 SDC-.5-2 Bosmina 4.50 1.8 SDC-.5-3 Bosmina 5.34 1.7 SDC-1-1 Bosmina 4.52 1.4 SDC-1-2 Bosmina 6.58 1.9 SDC-1-3 Bosmina 5.57 1.8 SDC-2-1 Bosmina 20.39 1.8 SDC-2-2 Bosmina 3.42 1.0 SDC-2-3 Bosmina 2.92 2.1 SDC-2-4 Bosmina 9.54 1.8 SDC-4-1 Bosmina 2.85 1.7 SDC-4-2 Bosmina 2.12 2.1 SDC-4-3 Bosmina 3.43 1.4 SDC-4-4 Cyclopoids 45.18 1.6 SDC-7-1 Polyphemus & Bosmina 8.51 1.8 SDC-7-2 Bosmina 10.04 2.2 SDC-7-3 Bosmina 25.75 2.0 SDC-7-4 broken SDC-7-5 Cyclopoids 5.61 1.9 Overall Average Diversity Inc 1^^ 1 a IcA — — — — — — """*" J..O 39 per liter for each sample. Zooplankton patchiness is evident, although not as pronounced as the phytoplankton patchiness. Ue cite stations NDC-2-1, NDC-2-4, DC-6, NDC-.5-1, SDC-2-1, SDC-2-2, SDC-2-3, and SDC-2-4 as examples. 40 A, 7 Study of Benthic Organisms Benthos Techniques Benthic organisms were collected by use of the ponar grab-sampler. Two grabs were combined and passed together through a washing device in which the benthic organisms were retained on a 0.5-mm mesh screen. In subsequent count- ing, the counts were divided by two to give the average of the duplicate samples. Organisms from the washing device then were collected into pint mason jars, labeled internally and externally, preserved with buffered formalin, and re- turned to the laboratory for processing. In the laboratory, the samples were concentrated on a small mesh net, and transferred with minimum fluid to the counting tray. For general survey purposes , the benthos are counted into the groups : amphipods, oligochaetes , sphaeriids, chironomids , and others (mostly leeches and snails). The averaged counts were converted by standard factors to give numbers of organisms per square meter. The counted samples are preserved by appropriate standard museum techniques and retained as a reference collection. We are well aware of some weaknesses in our treatment of benthos collec- tions. We know that sorting and counting into family groups as outlined above is a compromise between the desirable identification to species and the time- wise impracticality of such identifications to species. For the same reason, another compromise has been necessary to expedite enumeration of the oligochaetes. These worms tend to fragment during processing, and it is not possible to rapidly distinguish fragments from whole individuals. Therefore, to estimate oligochaete abundance, all worms and parts of worms were counted, and the total 41 divided by three. More detailed examination of some samples has shown that this factor actually varies from sample to sample, but we feel that our proce- dure is adequate to distinguish any major features of oligochaete distribution. We have tried the computation of diversity indices from our higher-taxon separa- tion of benthos, and have found that they are unrealistic; diversity indices will not be applied to our benthos collections for this reason. In view of the necessity to maintain continuity of method, we will retain the benthos-handling routine outlined in the preceding paragraphs . Benthos Abundances The abundances of benthic organisms collected on 10 July 1970 are presented in Table 10. In this table the collections are arranged into six parts, each of which contains stations at different distances from shore and in roughly the same water depths. Location of stations by distance from shore is navigationally convenient and desirable. It bears upon biological collections through its ef- fect upon water depth, which in turn bears upon biota through its effects on wave action, bottom stability, food materials remaining in the area, and other factors. The bottom in the area of Cook Plant is gently, but not uniformly, sloping and its sand is known to move with storms and currents. Because of this, the variations of depth at given distances from shore contain transient factors due to bottom movement. The two deepest stations were characterized by fine sediments with a high proportion of clay, indicating the occurrence of a sedi- mentation boundary at approximately 130 ft. As a whole, benthic macrofauna increased strongly with depth between 15 and 80 ft. This was also true of the major taxa amphipods (represented by the single 42 2 TABLE 10. The Benthos Collections of 10 July 1970. Numbers Per Meter . station Depth (ft) Amphipods Oligochaetes Sphaeriids Chronomids Others Part 1. Stations 1/4 Mile Offshore NDC-7-1 22 8 17 86 8L NDC-4-1 17.5 295 NDC-2-1 18.5 no data NDC-1-1 18.5 17 504 NDC-.5-1 20.5 26 113 DC-1 19 86 399 8 452 17L SDC-.5-1 19.5 226 SDC-1-1 13.5 278 SDC-2-1 18 17 660 SDC-4-1 14 8 95 SDC-7-1 14 26 8 86 Avei rages Stations 11.6 1/2 and 3/4 44 0.7 254 2.3 Fart 2. Mile Offshore NDC-7-2 27.5 86 982 69 86 l7L NDC-4-2 29 339 521 165 147 NDC-2-2 21.5 missing NDC-1-2 33.5* 747 1,817 321 113 8L NDC-.5-2 26.5 missing DC-2 40.5* 1,582 547 243 147 SDC-.5-2 28.5 156 686 113 60 8S SDC-1-2 40* 1,417 2,556 730 243 34L SDC-2-2 27.5 121 843 139 165 SDC-4-2 37.5 95 765 78 269 SDC-7-2 26.5 139 1,104 121 147 8S Averages 520 1,091 220 153 8.3 3/4 mile offshore. 43 TABLE 10 continued Station Depth (ft) Amphipods Oil gochaetes Sphaerlids Chronomids Others Part 3. Stations 1 1/4 Miles Offs hore NDC-7-3 48 556 573 26 43 8L NDC-2-3 51 139 1,104 34 34 8L NDC-.5-3 56.5 78 3,738 1,886 104 17S 52L DC-3 56.5 5,382 860 956 121 SDC-.5-3 54.5 2,278 2,608 217 34 17L SDC-2-3 51.5 721 8,781 921 69 17L SDC-7-3 51.5 1,704 660 104 130 Averages 1,550 2,618 592 76 17 Pcxrt 4. Stations 2 1/4 Miles Offshore NDC-7-4 52.5 495 78 34 NDC-4-3 55.5 1,973 78 34 43 NDC-1-3 57.5 4,243 3,199 1,982 513 104L 43S DC- 4 65.5 695 3,869 2,634 486 208L SDC-1-3 61.5 3,756 1,165 652 130 52L SDC-4-3 59.5 547 2,825 921 78 52L 43S SDC-7-4 53.5 4,573 1,747 226 8L Averages 2,326 1,852 926 179 73 Part 5. Stations 4 Miles Offs hore NDC-7-5 71.5 1,843 5,695 1,121 252 78L NDC-2-4 74.5 10,451 6,008 278 52 DC- 5 79.5 7,668 4,590 704 43 26L SDC-2-4 72.5 2,825 4,617 1,356 286 34L SDC-7-5 70.5 2,425 2,825 947 295 95L Averages 5,042 4,747 881 186 47 TABLE 10 continued 44 Station Depth Amphipods Oligochaetes Sphaeriids Chronomids Others (ft) Part 6. Stations 7 Miles Offshore NDC-4-4 134.5 5,208 5,686 DC-6 130.5 939 1,365 SDC-4-4 102.5 1,199 5,625 617 104 34M 147 34 17L 573 52 34L 17S Averages 2,449 4,225 446 63 34 45 species Pontoporeia af finis ), oligochaetes , and sphaeriids, Chironomids were present in low abundance over much of the area, and dominated the benthos in depths less than about 20 ft. On 10 July 1970, the water depths at 1/4 mi from shore were slightly deeper directly in front of the plant site. This condition was reflected in higher (but still low) benthos collections directly in front of the plant site (see station DC-1 collections in comparison to collections at adjacent NDC sta- tions north and SDC stations south of the plant site). To a lesser extent, this condition is shown also in Part 2 of Table 10, though it should be noted that stations NDC-1-2, DC-2 , and SDC-1-2 were at 3/4 mi from shore, while the rest were at 1/2 mi. The tendency for deeper water and higher collections con- tinues to be evident through Part 5 of the table, which presents station col- lections at 4 mi from shore. It is not present in Part 6, which gives collec- tions at 7 mi from shore. At present we do not know whether the increased depth in front of the plant site is a temporary feature due to transient condi- tions of bottom movement or a permanent feature. In spite of the deeper water in front of the plant site, the benthos col- lections at 1/4 mi from shore clearly demonstrate a relatively sterile zone or relative biological desert there, populated mainly by chironomids. If the Cook Plant outfalls go in at the planned 1160 ft from shore, they will be in this relatively deserted zone, where there is little benthos to be damaged. If the outfalls were to be placed at 1/2 mi from shore, they still would be in an area of reduced benthos. 46 The Benthos Species List Detailed study of the benthos reference collection from this survey has been carried out and the list of resident benthos of that day has been prepared. It is presented in Table 11, Altogether, 38 kinds of benthic macrofauna were distinguished, and many of these were identified to the species level, or larval type in the case of chironomids. The only animal listed as a single taxon, but which included several species, is Pisidium . Comparisons of the types of chironomids and oligochaetes with other areas of the Great Lakes revealed a mixture of "oligotrophic" and "eutrophic" con- ditions (as defined by indicator species) in the Cook Plant area during this survey. Future publications will deal with these relationships. Application of the diversity index to the samples provided no additional insight into the ecology of the area, for the values varied so much that their significance was ambiguous. The cause of many of the low diversity values was the high proportion of Pontoporeia in the samples. 47 TABLE 11. Species List, Cook Plant Benthos Survey July 1970 Arthropoda Crustacea Amphipoda Pontoporeia af finis Mysidacea Mysis relicta Insecta Diptera Chironomidae (larval types, not species) Chironomus f luviatilis-group ^. anthracinus-group C^. halophilus-group Kiefferulus sp. Crypt o chi r o nomu s sp. 1 . CO CJ in 4-J V^ ^ 'd • rH cd o a o •H o u cd CM CO o rO CO CX) CO iH c O d •H -H J-4 Cd CO M-l ,Q CO iJ >^ >^ O rH 'O u a vD CO a •p u :i >. cd Q) QJ 1 CM ^ M-l }-i O N o (U ;3 CU CJ VO ^ cd iH cd • iH rH u P rH S c^ CV4 a o ^ 00 O rO bO CJ o MH in o CO +^ iH a O i-> CO ^ >> 60 'd cd cd iH cd rd p CO CO o a •H «4H Cd u Q) MH > O Cd >mH U rC >^ c Cd O CO CO CO o ON in r^ 1 in u in Q iH o P^ >> H 13 h a 4-» :3 >. M CU 0) >-< O N CX) tH :3 CU Cd iH Cd • •H rH M P. CJ ^ -d- 6 rQ W) CJ o (U e rH 4J 'H a CO g CO rP CO CU p. < C/) ^ ffi P-. Csl 1 in CJ ^«H j>% a cd i+H Cd Cd H CO rH g Cd cd 00 >> CU rH cd O O CO +J CU § ^3 rH C O P •H -H J-i Cd CO UH ^ CO 5^ P +J CU 3 'd rH a o a •H 'H }-i cd CO ^H ^ CO tH 00 I o a in p rH CN CO rH CO U -H •H rH O •H U CO e r^ 00 CD o CT^ CD CM O C7^ ■P CU 5 TJ nH a o a •H 'H J^ Cd CO i+H rQ CO o •H Cd 4J en C +j O MH •H 4-» # a 'd 4J H CU CU x: CO j-i CU oc W •H a •H u P C/D CU CU # ffi ^ (U 'd •d +J a a Cd cd •3 •H •H CU (U H 1^ :2 en rs o v< CO o •H rH P o u •H rP u a CU a +J CU cd en 1^ o +j o l+H u (U * » U CU ,i2 CU cd u 4J p rs :3 P >- ^ CU H (U cd P a u B cd CU u o IH p CU p M € p 4J r> S cd o en H :2 PQ 50 o o <1 CO 1 1 CM 1 CX) u -sT p CM ^ rH 1 CM 1 <■ U CO P CN ;z: rH 1 CM 4J o 1 rH M P :3 o ^ a\ CO 1 1 o o o p . C o t>. CO rH W)^ Q) 0) -sT o 4-» M nd Xi in •H iH :3 0) • • iH CO a XI ^ • rH -H rH M O rH •H O CO a cu -d- w e ,j=> 60 CM uo CO O CO CO P. C O iJ 4J ^ c >. >> >> U tw 0) Q) CO 42 r-\ nd >^ J-J a o iH rH TJ P. *J :3 >> ^ 42 Q) ^ 4-i CJ MH p^ 42 >. § o CM in MH 42 ^^ U >. ^ in •H rH ^ Q) • • rH a (2 CO a rH C Q> u CO rH C c iH 'H iH u O r** CO a CO rH 'H •H CO > Q) M •H •H CO ^ CO e 43 W) CM in 42 -H u O MH CO CO O > 60 CO M-i CO 4-» u MH 42 >^ a o >. tobX O Q) in in U Q) '2 CX) •H 7-i ^3 0) • • 'i 3 S r-\ 'H rH M o CO •H 'H CO CM CO e 43 W) CM CO CO MH CO rC CO ■p •H CJ 14H ^ a a o >. d CO in rff S S ^ CO CO a •H 42 >. W) r^ 0) 0) o 00 in 0) >. 0) 3 TJ 42 5 M cO V-i Q) O •H rH :3 0) • CO rH c o a 4J o J-i 4-) rH 'H rH v^ o so ^ -H •H Vj CO •H a MH O cO U (U H CD CO p a u 6 CO cu J-I o MH P 0) 4-J :3 (U CO o c^ H :s PQ 51 CO 1 1 1 1 P ^3 1 1 1 Csl CO Csj 1 1 1 Q 13 CO I I p U o iW >^ CO CM o u J-i Q) 'd • • tH cd -d a C O CX) •H o C •H CO CNI -d- CO a Cd m CO . rj v£> uo u 0) § X) • • rH C O C o r^ •H •H J-i cd CNJ CN 4-J CO MH rQ CO U M-l Q) >^ CO CX) O 4-J J^ nj • • iH cd C o ^ J>. U •H cu tH tj V4 a o in 4J iH +J 13 >^r^ cd Q) (U vO • Cd 4-> >^ -u >. J-i O N O m cu ;3 0) • -d- rH M-l cd •H Cd Cd rH CO O rH rH J-l cr» CO (U O U V-i iH pL. O ^ O ^ O ^ W) rH tH 60 CO W) W) O LO 4-» u U-l rC P^ o >> cj tiOM cu CU G\ in •P Q) ^ 'O CM •H tH :3 cu m • ^H C o a • rH -H iH u a^ LO *H 'H u cd -d- htly s y m nish b rH u o in M-4 CO M-l Xi CO CO 0) rH :3 W)^ ^ cu CJN o 4J CU TJ X^ a CO •H rH O (U • • iH C c u ^ cd • tH -H M M C7^ a\ •H -H cd •H CU }-< CN Ui B r^ txO rH CM CO m CO :^ M-l W) CO 4-» rC 4J CO CO 4-» O Cd •H U 14-J s C o >^ c c o 1^ 3 § ,i*i 4-J }-l >.tH M ^ Q) C7^ in U -H -a M-l (U N O in iH O 0) • • iH Ti O a iH > Cd o • •H J-4 J-» ON r^ •H CU J-4 Cd rH iH o ^ o rH e ,o w) rH rH CO B ^ CO CU •H d o o x> a cu a. cu < I Cvj I p o 4J M-l C 4J O IW •H J-J • O 'd U H CU cu X CO U cu w a w •H a •H o P cyD CU •H tn 4J cu Xl TJ Cd a c C cd JJ •H •H •H » C o >. a W)ri^ CU CU CO in U Q) ^ T3 in •H rH :3 CU • • rH a O C • rH -H rH M ON 0) H p M 5 cu 4J u 4J cd o IS pq 52 1 1 1 r^ CJ • 1 00 U iH P r^ C/3 iH 1 00 U o P a^ W o 1 1 in v>l 1 o u tH p r^ en «H in I I CJ P 13 I P 00 o o o o m CO ■p iH u IH H >. J-» a o ^ >. g M rfi 0) 00 in 60 4J ^XI ;3 H CvJ tH o cu • • .H a O a u Cd a O •H }-• }-l iH ^ d a o >^ cj ri^ 5 cu 00 in +J (U ^ t^TJ in rH O (U • • H a O cd C • •H M }^ o 00 •H -H U V-i cd CO e ^ 60 CvJ CM 4-» CO M-l iH 4J (U 60 CO >^ -P a o rC >^ CO Cj i?^ cu l a^ a\ rH -H o M cd iH e H CO 60 iH o H M-l CO CO a ,n CO -5^ S . CO cu vO in M-l cu >-i >^ >. (U • rH C a (U cd Cd a a\ tH cd t\3 •H > u rH -H tH 4J ^ 4J M-l o 60 CJ MH u M-l g >. 13 vO in U *r{ 'd • iH -d a O CM •H (U CO eg in CO e CO CJ 4-> O MH •H 4-» « O -Td H H Q) -i CO o •H r-\ P o o •H ^ >-l a (U o 4J cu cd en rs CJ H o MH U Q) * #< +J i u > >^ TJ ^ g c o 0) 0) .^ § (U 00 in M-l ^ >^ 0) T? u >^ P^ CU TJ o iH O 0) • • iH o C CO c c 0) cd Cd a C o *d J^ u o ON cd c CO iH vH CC > J-i iH 'H cd u e r^ W) CM LO 43 •H 4-» a m CO o W) O M-l CO CM 1 1 u p CO o en o CO cd u u >. > cd o ^ u cd o o •H J^ e to o O ID CO iH C O S •H -H J^ cd CO <4-i rO CD 1 1 in u tH Q 00 C/^ o -si- 1 1 CM 1 0^ O in Q 00 en iH o CO cd o (U > cd Cd o ,i2 :5 o CM O O CM o CO u Q) cd o a 'a p. cd G a ^ (0 CO o iw M c o >^ cd (U 0) o in m ^ 4J (U TJ o a; :3 0) • • rH U C iH a c • iH iH >-l Oi CM Cd C cd •H "H Cd vO o ^ W) rH r^ rC .H +-> CO M-l CO J-l >^ CU 4J 'O cu Cd a iH a > >-^ 'H 'H cd O CxO ^-4 CO CO CO 1 CM 1 CJ Q C/3 CO 00 o u CO Cd u u >, > Cd O rC in CO rH O •H V^ 6 rO 0) u u o CT» CD CM 4J in in CO o •H CO CU ^T^ rC >^ CU -d rH rH o a C •H ^ -H Cd "H CO rQ ^ CO 15 Cd C C o ^ 'H Cd C W) 'W CO CM 1 1 CM 1 CM U CM Q 00 CO r^ ^ e a ri«i 5 0) 00 rH O 0) • •H V^ M CO e rn W) o CM CM m CM rH C O S •H 'H M Cd CO M-i ^ CO 1 CM 1 m O CO Q 00 C/D o vO rH t>0 (D •H 'H }^ e rH W) O CNj o 00 rH J3 •H -H 0) rH CI4 Cd CO ^H ^ CO CO (I) :3 •H +J a o o T3 CO 1 rH 1 CO u p 00 C/D rH H d CO W •H • 4J (1) Cd 4J ^ to H o CO c 4J M-l •H 4J # 'd +J 0) 0) ^ M > d a rH O cu •H J-i M o u CO •H r-\ P u •H rC u cu p CD cd CO 1^ o 00 o CM m rH cu Cd ^ cr«H u 4J IW M cu • ^ cu rC *J (U H (U cd p u g cd cu u iw p. CU ■u ^§ 4-» 4J cd CO H :si pq >. > >^ J-i tH 'H cd W) a m CO 54 I Q CO CN m o CN r^ ^ u M Cd Cd TJ T3 o O o O o 00 M-l in o ■M (U 3 na iH c o a •H 'H V^ cd CO m ,£3 CO I I Q CO CM U Cd o o u u Cd o o 4J 00 o CM in en in rH a b CJ •H 'H J^ cd •H CO IW ,iD CO 15 13 ^ >. > p in 4-> Q) ^ 13 • rH d O a vD •H -H u cd CN CO M-l ^ CO (1) d •H d o a X •H 13 d 0) I I o P CO I I u p CO CO Csl o CN Cd 13 o o d 4-» o 1+-I •H 4-> #^ O 13 +J H Q) 03 rC CO U 0) W W •H P •H P CO 0) » ffi (U 13 13 6 d d Cd •H •H •H 0) H :2 :2 CO u cd 13 o o o >-l CO o •H .H P O CJ •H ^ u O (U CJ 4-J >. rt o +J 0) 5 13 • fH d o d ^ 13 4J a >. d cd d M-l d cd cd M cd •H tH CO bO >. CO in a 0) • m (U >-i t>. nH -H iH rd +J M-l O O M-J iJ o MH M o (U * • JJ CD rC 0) cd j-^ u P :^ :3 Q. > 4-J 0) H 0) cd P O J-i g cd QJ u o M-l Q. 0) 4J 13 S 4J 4J Cd O CO H 12 pq 55 Appendix B PHYTOPLANKTON COLLECTIONS, 10 JULY 1970 Identification of Plate Components Top line (left to right) Station number, number of species or groups, total number of individuals per milliliter, and the diversity index of the collection. Columns (left to right) : First Names of species or groups collected. Second Numbers of individuals of each species or group, per milliliter. Third Percentages of the total individuals that are represented by the individuals of each species or group. These are the N /N factors used in the diversity in- dex equation. 56 ir\oooooooo^oo^o oo (rk>omCOCe«-«Ctf>«-4 • •••••••••••••••••• •^0©OOOOOc«MQCCOCOCC c •-4 • 1^ IM p^ ^ ^ OMTN 0» *NI flO *^ -^ •-• in *N» f^ ^ •-♦OJCM »-• h- «M ' «m < I/) 3 < K X a. a UJ z , . Z I/) D ^^tU UJ 3 M Ciu. z o • ec «/» n u» o. 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