aN SN ~ s eS * WARS SN SSN AS AN NN BY Se AS SS " AN S aa eel ~~ SSNS Ree SNES ASS Y SANE. SN WN OY WS RY AX RAS WALS OS Sek RP ‘ REN Tee Avie w aes ARENT SS.' SSS aa a FROM THE Library of Rhoda A. Esten A teacher and supervisor in the schools of the City of Providence from January, 1878, to May, 1901. Donated to this Library under tbe provisions of ber will. - ee MOE 1OHM/18WW ous - Sh iow Sa ee oe! alia . ye ter yea Were a ete tae a. he, ANALYTICAL KEYS TO THE GENERA AND SPECIES OF THE FRESH WATER ALGE AND The Desmidiee of the United States, FOUNDED ON THE CLASSIFICATION OF THE REV. FRANCIS WOLLE’S MONOGRAPHS, BY DR. ALFRED C. STOKES, Author of “Microscopy for Beginners;” “A Contribution toward a Natural History of the Fresh Water Infusoria of the United States,” etc. PORTLAND, CONN.: EDWARD F. BIGELOW, PUBLISHER. 1893. Entered according to act of Congress, in the year 1893, by L. F. BIGELOW, Publisher, in the office of the Librarian of Congress, at Washington. Sto CONTENTS. Explanation of Plate, - - Value of of Mikrons in parts of an inch, Introduction, : - - Key to the Classes of the Fresh-water Alga, Key to the Genera of the Fresh-water Algz, Key to the Species of the Fresh-water Alge, Key to the Genera of the Desmidiez, Key to the Species of the Desmidiez, Glossary, - : - Index, ~°*- . . . Value of Microns (/) in fractions of an inch. fd Inches.| yw Inches.| ym Inches.| yw Inches.| yw Inches. I,.0=I-25000 | 18.0=1-1383 | 45.0=1-533 | 79.0=1-316 | 113.0=1-221 * 1.5=1-16666 | 18.5=1-1351 | 46.0=1-543 |80.0=1-312 | 114.0=1-219 2.0=1-12500 | 19.0=1-1315 | 47.0=1-532 | 81.0=1-308 | 115.0=1-217 2.5 = I-10000 | 19.5 =1-3333 | 48.0=1-521 |82.0=1-304 | 116.0=1-215 3.0= 1-8333 | 20.0=1-1250| 49.0=1-510 |83.0=1-301 | 117.0=1-213 3.5 = 1-7222 | 20.5=1-1214| 50.0=1-500 |84.0=1-297 |118.0=1-211 4.0==1-6250 | 21.0=1-1143 | 51.0=1-490 |85.0=1-295 | 119.0=1-209 4.5=1-5555 | 21.5=1-1162 | 52.0=1-480 |86.0=1-291 | 120.0=1-208 5-O=1-5000 | 22.0=1-1131 | 53.0=1-471 |87.0=1-287 | 121.0=1-206 5-5 =1-4545 | 22.5=I1-1111 | 54.0=1-464 | 88.0=1-284 | 122.0=1-205 6.0= I-4333 | 23.0=1-1087 | 55.0=1-454 |89.0=1-281 | 123.0=1-203 6.5=1-3846 | 23.5 =1-1064|56.0=1-446 |90.0=1-277 | 124.0=1-201 7.0=1-3285 | 24.0=1-1042 | 57.0=1-438 |91.0=1-274 | 125.0=1-200 7.5—=1-3333 | 24.5=1-1020 ,58.0=1-431 |92.0=1-271 |126.0=1-198 8.0=1-3125 | 25.0=I1-1000 | 59.0=1-423 |93.0=1-268 | 127.0=1-196 8.5=1-2941 | 26.0=1-961 |60.0=1-416 |94.0=1-266 | 128.0=1-195 9.0=1-2777/27.0=1-926 |61.0=3-409 |!95.0=1-263 | 129.0=1-193 9.5 =1-2631 | 28.0=1-893 | 62.0=1-403 |96.0=1-260 | 130.0=1-192 10.0=1-2500! 29.0=1-862 |63.0=1-396 |97.0=1-259 | 131.0=1-191 10.5 =1-2381 | 30.0=1-833 |64.0=1-390 |98.0=1-255 | 132.0=1-189 II,O=1-2272 | 31.0=1-806 | 65.0=1-384 |99.0=1-252 | 133.0=1-188 II.5=J-2173|32.0=1-781 | 66.0=1-378 | 100.0=1-250| 134.0=1-186 12,0=1-2166 | 33.0=1-787 |67.0=1 373 | 101.0=1-247 | 135.0=1-185 12,5 =1-2000 | 34.0=1-706 |68.0=1-368 | 102.0=1-245 | 136.0=1-183 13.0=1-1923 | 35.0=1-714 |69.0=1-390 | 103.0=1-242 | 137.0—1-182 13.5=1-1851 | 36.0=1-694 | 70.0=1-357 | 104.0=1-240/138.0=1-181 14.0=1-1785 | 37.0=1-675 | 71.0=1-352 | 105.0=1-238| 139.0=I1-179 14.5=1-1724 | 38.0=1-657 | 72.0=1-345 | 106.0=1-235 | 140.0=1-178 15.0=1-1666 | 39.0=1-641 | 73.0=1-342 | 107.0=1-233| 150.0=1-166 15.5=I-1612 | 40.0=1-625 | 74.0=1-337 | 108,0=1-231|175.0=1-142 16,0=1-1562|41.0=1-609 | 75.0=1-333 | 109.0=1-228 | 200,0=1-125 16.5=1-1515 | 42.0=1-593 |76.0=1-328 | 110.0=1-227 17.0=1-1470 | 43.0=1-583 |77.0=1-324 | I11.0=1-225 17.5=I-1421 | 44.0=1-567 | 78.0=1-320 | 112.0=1-223 Explanation of Plate. [FRONTISPIECE.] GENERA OF DESMIDS. Each Figure is numbered to correspond with the number of the genus, FIG, CON AKHAW D4 IO, Gonatozygon. Omitted; the cell needs no figure. Genicularia. Hyalotheca. Bambusina. Leptozosma. Omitted; the cell needs no figure. - Desmidium., Phymatodocis. Sphzrozosma. Onychonema. Mesotznium. Spiroteenia. Penium. Closterium; three figures, showing central inflation and ends produced into beaks. Docidium. Triploceras; end and part of cell. Calocylindrus. Cosmarium. Tetmemorus. Xanthidium.. Arthrodesmus; 20a, front view, 4, end, view. Euastrum. Micrasterias; five forms. Staurastrum; four forms; 23a, front view, 4, end. Pediastrum; three forms. INTRODUCTION. To know the origin of any object, event or act, is always of in- terest. To know what lead up to a certain acceptable innovation or aid is pleasing to everyone, and often important to the historian of any department of the world’s work. What was the form of the first analytical key, who made it and what induced him to make it, are in- teresting questions that the writer at least cannot answer. Such tables have become very popular in the various departments of. bot- any and of zoology, but who originated them is not known; nor when the first was published, nor where. Like many other good things, these keys may have been the result of simultaneous thought and experiment on the part of several workers that felt the need of some outside aid to the inside treasures of the ever increasing mass of scientific knowledge. But to whomsoever belongs the credit of or- iginating these devices, to him belong much praise and many hearty thanks. That analytical keys were needed is proved by their popularity. They are welcomed by the student in all the sections into which Na- ture is for convenience divided. It is true that there are some ob- servers who scoff and laugh in derision when “keys” are mentioned, but these few belong to that class, fortunately limited, whose mem- bers are unable to usethem. There are persons that seem to be de- ficient in those mental qualities that make the use of such tables a pleasure and a delight, as there are others unable to arrange even the simplest of keys. If in a scheme of the kind there are three groups, a blue, a black anda green, these unfortunate opponents of keys seem unable to decide what to do next, when the specimen hap- pens to be green whilethe first description in the list calls for a blue ‘ 12 INTRODUCTION, object. That they should glance down the list tosee if by any chance a green specimen may have been provided for, does not oc- cur to them; they toss the paper aside, and laugh at the utter folly of the statement that any intelligent person should be able to use a key. The truth is that inability to use these valuable aids is an evi- dence of adefect inthe mental organization. Such critics are forced to page through the monographs they attempt to study, with great loss of time and with much labor, to find the information that may be wanted; whereas with the object and a key to its class in hand the labor becomes a delight, and the result a valuable acquisition, be- cause no key to any department can be used evento a limited extent without teaching the student many facts that he might otherwise have overlooked. A table of the kind will draw attention to the morphology of the object in a way that will lead not only to its proper classification, but to something much more important, the enlarging of the stu- dent’s powers of observation. Some of those that oppose the use of artificial keys are themselves compelled not only to page through an unfamiliar monograph, but in many instances to scan the pictures for the light they are’ groping after; and failing pictures they guess as to the character of the object from its resemblance to something re- motely similar, and in the end, at great expense of time, and perhaps of temper, may get aclue that may lead them to the goal they have been so laboriously seeking. With the intelligent use of an artificial - key the place, the character, and even so unimportant a matter as the name, can be rapidly obtained, and the student be led on to fur- ther study and investigation. It is always encouraging to feel that by one’s efforts a way has been opened to further conquests, or even that a path has been cut to some previously unknown view. This the use of artificial keys always accomplishes. When the beginner feels that he can do some- thing unaided he is inspired to do more. When he feels that he has travelled alone through some pathway in some department of natural science, he is anxious to go further. One step taken with the aid of an artificial key is sureto be an encouragement toward another. The powers of observation are increased. The ability to balance one thing against another is cultivated. In a word, an artificial key can be made one of the most important aids in the mental development of the beginner that can be imagined. The ob- server that scoffs at these aids, the observer that refuses to take advantage of them, is doing a foolish thing and losing a good one. INTRODUCTION, : 13 There are two distinct varieties of these valuable aids, the nat- ural and the artificial. The former is founded only on the charac- ters that the advanced scientist uses in his classification. These are often so exceedingly natural that to learn the proper position of the object it must be destroyed. To learn something of an animal through them the animal must be dissected, and the anatomy of its nervous system, and the morphology of its osseous system, and the structure of its heart be accurately observed before it can be classed. These arrangements may be, they often are exceedingly valuable to the advanced scientist; to the amateur and the beginner they are terrible. They haunt his dreams like horrid night-mares, provided he is incautious enough, or ignorant enough to attempt to use them. I have not a word to say against such analytical tables. I commend them for use in their proper places. They are important for their purpose, but that purpose cannot be the beginner’s or the amateur’s. It is the learned man that can delight his soul with such a scientifickey. To him itisinstructive and helpful. Toany one else itis a bugbear anda horror. The beginner is not prepared to begin with the nervous system of his ‘‘find,” or even with the structure of its heart. He must have something nearer the surface and more easily seen than are these parts. His key must deal with the external and the evident characters. It is for him that the artificial key is in- tended, and to him it is inspiring and helpful. This second kind of key, this artificial kind, is as its name indi- cates, founded not on what the advanced investigator would use in his classification of the animal or the plant, but on some obvious, prefer- ably external, points that may be of no use in the art of classification, but that can be used as crutches to help the student over the miry and the stony ground to the hills of science where he will be ,able to look back and smile at his former helplessness. These artificial keys have this for their object, and only this. They aim to help over the hard places; to encourage the user to go further, and ‘to do mote, so that finally he may become an expert, when he will. no, longer need any but the natural aids, or may even get along without any other assistance than skilled eyes and a “learned touch.” The beginner, however, must have a guide, especially if he be trying to work alone. This is true in every department of natural science. It is especially true in microscopy. The field is so im- mense, the outlook so vast, the work so apparently endless, that with- out an aid the beginner at once begins to flounder in his sea of troubles, and before long he sinks discouraged to rise no more. He ~ 14 INTRODUCTION. becomes so disheartened since everything is so new and strange, and there seems to be no one within call to lead him out of the difficult- ies, or out of the embarrassment of scientific riches, that he is entirely discouraged, his enthusiasm is lost, and with this loss is abandoned a study that would have lessened his cares, increased his joy in living and lengthened his life. The beginner working alone wastes much time. ‘To be sure, he may before long learn that a green object that stands still is a plant, or may be; and also before long he hears that all green microscopic objects that stand still are not always plants. If he have a teacher his condition is a more favorable one; but if he be alone he must have an artificial guide, or fail. For such students the artificial keys are providential. | Once introduced to them he is ready to go on to new conquests, The use of such tables in the popular treatises on botany has done more to popularize that beautiful study than a score of learned monographs on the subject. As soon as the pupil learnsthat he can by his own efforts obtain even the bo- tanical name of his plant,a feeling of enthusiasm fills his heart, and he is eager for another specimen to analyse. And in the analysis he is learning the principles of classification, the structure of. the specimen and the function of the various organs. By means of the key he goes with little trouble and waste of time to the order, there he finds another key that leads him pleasantly to the genus, and perhaps a third that takes him to the species. There he finds the plant exactly described. He has had the prominent characters brought to his no- tice in a pleasing way, and the final result is that he not only learns to love the science of botany, but that he soon learns to know at a glance, without the use of the artificial key, to what order a strange specimen may belong. He has become scientific without knowing “how he*became so. Hehas travelled by the royal road. It is so, or should be so, in the science of microscopy, to call for convenience a ‘sciéncé that which is really a combination of all the sciences. If every department of microscopy could have an artificial key to open the doors of its treasure-houses, the amateur would have a hap- pier time, science would be benefitted and the maker of the key, the scientific artisan, would himself be blessed both in this world and in the next. Such a pleasing state of affairs can not be attained. The doors are too numerous to be opened by a single key. To make the proper number is not possible for one worker, however willing he may be to try. Each specialist could forge a key to the doors or to the dark passage-ways of his special scientific castle, and offer it to those that would follow after himif they could. But the specialists are not INTRODUCTION. ace C disposed to become scientific blacksmiths, and to forge keys to the treasures they have locked up in their formidable monographs. In- deed, it is not possible that they should. Theyhave no time to make the entrance ways easy and agreeable. They are after the facts of the science; they are seeking the internal secrets of the creatures they have studied and written about; they are content to leave the en- larging of the building to other specialists that may come after. It is no fault of the writers of learned monographs that they put forth their treatises without a single loop-hole of entrance for those that would, if they could, enter in to see some of the treasures of which rumor has told. When a man has spent his days and his nights and his earthly and bodily substance on the production of a treatise that shall make his name known to the world, he is naturally disin- clined to labor longer and harder to make easy grades and smooth paths and shady retreats along the way to his treasure house; and when the learner has arrived.at the gates, the learned man is not dis- posed to stop his further investigations to throw a pretty key out of the window and ask the uninvited guest to come in and sup with him. He is too busily engaged in the new investigation that his completed investigations have made necessary. There is no end to the questions to be studied and decided. He cannot, muchas he may be willing, condescend to make keys for the beginners. If he makes any at all, ‘they will be of the severest kind and intended for his scientific equals only, not for the humble followers in his foot-steps. We should never blame the writers of learned monographs for not offering these gilded keys. They cannot. They have gone up too high to stop, for the higher they go the more there is to be done. Andthey that have climbed that high are the ones best adapted to climb higher, for they find it easier to go up than to come down. But if such books are to be used by any others than the rather limited class of experts that have the knowledge needed to find their way unaided through the pages, then a guide of some kind must be furnished. An index will not answer the purpose, as to use it de- mands just that information not at the disposal of the novice orof the amateur. All of these scientific treatises may be as useful to the modest student as to the learned investigator, provided, as I have so often said and repeated, some means can be devised by which he may be helped to make an intelligent entrance into its various depart- ments. Mr. Wolle’s monographs on the Algz and the Desmids are no exception to the rule. They are not adapted to the use of the ama- 16 INTRODUCTION, teur microscopist in the state in which the author has left them. It is not possible for the youthful microscopist to read the description of one hundred and twenty genera of Algz in order to locatea single specimen that he may find in the wayside ditch. To ascertain with any degree of comfort the probable resting place of the speci- men he must have some help. It is that help that this little compila- tion hopes to supply. By its intelligent use the student may ascer- tain the specific name of any fresh water Alga or Desmid in the ponds and the ditches of the United States. This he can do with the ex- penditure of the least time and labor, leaving them to be spent in the subsequent study of the plant, after he has had the satisfaction to learn what it is, and where inthe classification it belongs. If the worker has the name of the specimen, he then feels that he has a sup- port on which he may hang his subsequent information, and a starting point for further investigations. Not to know the name of the ob- ject and yet to try to study it, is like an attempted conversation with a stranger whose antecedents and local habitation are unknown. The effort is not an agreeable one. Theconversation languishes, and interest is lacking. The name and the habitat of the person add much to the pleasure of the meeting, and many suggestions to the in- terview. So the name of the microscopical or other specimen is al- most essential to the well being of the young student. The name is, too, about the first thing the advanced scientist tries to learn. It is impossible to do otherwisé. The object can not be re- ferred to by speech or in writing until its name is known; what other workers in other parts of the world may have said about it or done with it, can not be known until its name is learned, as without the name all indexes are closed in all the books of all the libraries. The name is the clue to further knowledge, its starting point, even the hook upon which further information is to be hung. Whatever advanced scien- tists may say to the contrary, their first effort, perhaps it is an uncon- scious one, but their first real effort is to ascertain the name of their new specimen. If it has none, they at once proceed to give it one. All the wild talk about the undesirability of learning the name is wrong in principle. ‘The name is, as every one will cheerfully admit, only of secondary importance when compared with a study of habits or of morphology, but it is as essential, since it is and must ever be the starting point for further investigations, at least on the part of the amateur. If he chooses to stop at the name, that is his misfortune; but even then he has gained something. If those whom I may be able to help by these keys shall do nothing more than identify their INTRODUCTION. A “finds,” I shall feel that Ihave done some little thing to help them and their friends. I shall feel pretty sure that the mere finding of the name will not be the end; but if it should be, where will be the harm? Is it acrime to know the scientific name of the plant or of the animal? Knowing that is knowing something, I think. Would you have the pupil dissect the nervous system before he has learned to recognize a nerve, or before he knows the muscles to which the nerves are distributed? Of course he might say ‘“‘This is a nerve,” and “That is a nerve and it goes to that muscle,” but would you have him carry the dead body about with him in order to dissect it and point out the nerves and the muscles that receive them whenever he wished to com- municate with you or with any of his friends in reference to the nerves and the muscles of that special creature? This is exactly the result which those that cry out against learning names would accomplish if their desires prevailed. No, no! Get the name the very first thing, if you wish. It will make a good starting point. It is not the end and the aim of any study; it is only the bridge that takes us over the swamp. . After awhile we will be big enough and strong enough to pass the swamp without the bridge, as after awhile we will be able to get along without an artificialkey. But do not condemn the bridge that has carried you safely over. The keys in this little book are as artificial as they could be made. In some instances they are perforce somewhat natural; that is, the fruiting plant has been used to the exclusion of the more frequent sterile condition. This, however, has been avoided as much as possi- ble, and in several instances two keys have been prepared to the same genus of plants, one for the fertile, the other for the sterile filaments. They are all, as the title page indicates, founded on the classification contained in the admirable monographs by the Reverend Francis Wolle on the fresh water Algze and the Desmids of this country. Un- til Mr. Wolle took up the subject our American microscopic plants had scarcely been touched, except by a few observers in a desultory way. Hehas brought order out of chaos, and has prepared two mon- ographs that are monuments to his learning, skill, care and patience. These keys are made not alone with the object of leading the begin- ner to the study of the fresh water Algz and the Desmids, but to in- troduce him to these fine works of Mr. Wolle’s. While the beginning mcroscopist in his study of microscopical botany may, toa certain ex- tent, succeed in identifying his “finds” with these keys alone, to do so with entire satisfaction he should have access to the monographs on the Algz and on the Desmids. The references at the end of the 18 INTRODUCTION, classes and of the genera are to the numbers preceeding those classes and genera in this list; those following the species are in every case for reference to the pages on which will be found the extended de- scriptions in the monographs already so often referred to, where ref- erences will also be found to the illustrations. The measurements of the Alge are given in parts of amikron (j); in the Desmids, by both fractions of an inch and of mikrons. The former will necessitate a little calculation, if the reader is not en- tirely familiar with the minute space called a mikron and represented by the Greek letter 4. It measures the 5,4,, of an inch in length, and the calculation needed to bring it to the fractions of an inch as given in the measurements of the Alge, are slight. Twenty-five mikrons, for instance, being 55,5 inch, or z;45 inch. It is only necessary therefore to use the number of mikrons as given with the Algez for the numerator of a fraction of which the 25000 forms the denomi- nator, and reducing the whole tothe lowestterms. Inthe Desmids the calculations have been made, and the measurements given in both forms. ‘The student will therefore need an eye-piece micrometer, but it may be ruled to parts of an inch. The use of the mikron, like the use of the metric system, has not and probably never will come into popular favor. Does the reader know how to use a key of this kind? To some this appears to be a mysterious thing, although a little inspection of the key itself should make it plain. It is simply a matter of exami- nation, comparison and rejection. With the specimen in hand, which in this instance means under the microscope, begin at the beginning of the key and compare the description in the first sentence with the object under the instrument. Do the two agree in every particular? If not, leave that reference and go to the next having the same letter or other symbol at its front. If that should describe the specimen, notice the letter in the parenthesis at the end of the line, and seek that same letter at the beginning of a line in some other part of the key. You will probably find several lines beginning with that letter. Starting at the first, compare the description there given ina word or two with the actual specimen, and if it does not agree pass to the line next below. If at the end of this line there is another reference let- ter in parenthesis, turn to that, and so continue until at the end of some line you find the generic or the specific name of the plant. Mis- takes are easily made, here as elsewhere. If you should happen to make one at the start every step forward will lead you further and further astray. With care and intelligent observation, errors may be INTRODUCTION, 19 avoided and the specimen readily run down to its proper place. Af- ter a little practice, the use of such artificial keys becomes a great pleasure. The benefit has already been referred to. Theonly secret of their successful use is to be certain that each progressive step is right before it is abandoned for the next. And after a little practice, too, the student will become so familiar with the Algz or with the Desmids that he will not need to apply to the generic keys for aid, but will be able toturn at once to the proper genus, and there use the keys to the species; and with a little further experience he will be en- abled to recognize at a glance the species, and then will be prepared to proceed with his investigations, or to refer intelligently to the ob- ject so as to communicate with his friends and fellow workers in re- gard to it. It is as impossible to speak or to write of an Alga or of a Desmid without using its proper name, as it is to speak or to write of your most intimate friend without using his. ) Alge and Desmids are singly invisible to the naked eye. It is only when they occur in large masses that the eye can take cogni- zance of them. It'rarely occurs, however, that the Desmids are so abundantly congregated that they thus obtrude themselves on the observer. When a large quantity has been collected and the vessel placed near a window, they will collect ina green film at the surface of the water on the lighted side, and there become visible in mass. In the ponds and shallows such an occurrence isnot common. At times they are found so abundantly that by holding a glass vessel of the water up to the light they may be seen floating about as minute green objects, which the trained eye will recognize and the pocket lens make distinct. But these varieties are among the largest of the forms; according to my experience they are always exclusively con- fined to the Closteriums. Other large forms, like JZicrasterias, at least in the writer’s locality, rarely occur in such profusion... To col- lect the Desmids, therefore, it is necessary to collect by faith. The microscopist can know exactly what he has only when he gets home and examines the water drop by drop under the microscope. With the Alge it is different. These are usually visible to the naked eye, as they are almost invariably collected in large masses floating on the surface, submerged just beneath the surface, or at- tached in waving tufts or fringes to sticks and stones and other plants in the ponds. ‘The eye of faith is not needed to recognize them. They usually force themselves on the wondering attention of the observing pedestrian in the wayside lanes, beside the ditches and slow brooks. As soft emerald clouds, or graceful streamers floating 20 INTRODUCTION. in the sluggish current, or resting like a green skum on the surface, they are readily seen and as easily gathered. No collecting tools are demanded for either Algze or Desmids, except a dipper of some kind, a common tin dipper is as good as anything, and a few bottles or other vessels to carry the treasures home, and to keep them con- cealed from inquisitive people; the collecting naturalist must always be prepared for a meeting with such persons. Many media have been recommended for the preservation of these beautiful plants. Thé trouble with the majority of these prepara- tions is that they will in time, some usually ina very short time, allow the cell contents to contract and to become so distorted that the object is worthless for any serious study, and worthless, too, as the object of beauty that it was when first mounted. Some of the commended media act well on some kinds of Algz and Desmids, but fail on others. The following are among the best, yet even these are not all adapted to all the different kinds. They are mentioned in the order of their excellence. CAMPHOR WATER.—This is made by placing a lump of camphor in distilled water and leaving it there for several days. Mr. W. H. Walmsley, anexpert preparer of microscopical slides, says that he has had a specimen of Draparnaldia in camphor water for twenty years, and that it is now as beautifully green as when first mounted, and that the chlorophyll, its green coloring matter, seems to be un- changed. When he desires to mount an Alga or a Desmid, he places it ina small quantity of camphor water to which a few drops of glyc- erine have been added. At first the plant will become lemon color, but after a few hours the original green returns in all its vividness, and then he at once mounts the plant in some of the fluid. } CARBOLIC ACID WATER, made by adding afew drops of the acid to a phial of water, is recommended by the Rev. Mr. Wolle, but he says that although this will preserve them for months or even years without deterioration, it may allow the coloring matter, the chlor- ophyll, to fade; this however in the case of the Desmids is of little importance. A SOLUTION OF CHLORAL HYDRATE, in the proportion of five grains of the salt to one ounce of water, answers well for some specimens. Like camphor water it has the merit of being easily made and easily used. A SOLUTION OF COPPER made after the following receipt is admir- able for some of the Algze, preserving the color and the form entirely unchanged, while on others it will not act so favorably. INTRODUCTION, 21 : 50 grammes; : : 50 grammes; - 0.5 grammes; - 2 grammes; - - 2 grammes. Camphor water, - - - Distilled water, - - - - Glacial acetic acid, - - - Crystallized chloride of copper, - - Crystallized nitrate of copper, - - Dissolve and filter. When mounting with this copper solution the cell should be made and the cover cemented down with shellac. With almost any other cement failure is pretty certain, as the cement will surely run under and ruin the preparation. After the shellac is hard, it isa good plan to add some of Brown’s rubber cement around the cover. This is entirely transparent and will make the mount more secure, and-at the same time add something to the beauty of the finish. TRENTON, N. J., 1892. Powe. fF Key to the Classes and the Genera of the Fresh-Water Alge. KEY TO THE CLASSES AND THE GENERA OF . THE FRESH WATER ALG. CLASSES OF THE ALG. § Rosy red, blood-red, reddish-brown, purple or blackish; many Se NE a ee Vw gel tN. ok aA) ig Aas § Chlorophyll-green, sometimes becoming crimson, purplish, flesh- color or yellow-brown; 1, 2 or many celled; often nucleated, CLASS IL. § Never chlorophyll-green; colorless or variously colored; 1 or many celled; in jelly when out of the water . ° ..'. 39>. CLASS 111: KEY TO THE GENERA OF THE ALG. CLASS I. @ Olive, brown or grey, black with age; filamentous, often hollow, bristle-like, rigid (A). “ Violet, violet-purple or bluish-green; filaments articulate, branched or not (E). “{ Purple; tufts loose; filaments r in. long, not branched (D). “ Purplish, smooth, forming a firmly attached coating to stones, flildebrantia, 7 { Brownish red, vermillion, or greenish with red centre; cells 4-8, free or in a short lived cluster . . . . . Chlamydococcus, 38 { Blood red, cells large, tegument thick, lamellose, stem ringed, Urococcus, 66 A. Filaments nodose, tufted; fertile filaments hollow, with internal axillary thread; in rapid water (B). a A. Filaments transversely banded or annularly constricted; with in- ternal central axis (C). ) 3 26 GENERA OF ALG&., . Axillary thread a single series of cells with transverse threads at regular intervals .. . oS Sin ete a a aE Se Axillary thread of cohering Rlamente: ie many whorls of mon- iliform branchlets So8 5 ENT aR Gin ARENA, ae . Simple or branched, slinnery: cells in a ieee layer, membrane thick, colorless . . Os ear mee ts” a . Branched; main stem often wane an extemal parallel series of cells; branches moniliform, clustered . . Satrachospermum, 4 . Branched; main stem without external parallel series of cells; branches not moniliform (F). Filaments articulate, villose, branchlets dichotomous . TZhorea, 5 Filaments articulate; branches not whorled; plant steel blue or PUP DUST Ma et OF Fag Cs ae Filaments articulate; brahoben gree: anfeaninn: plant olive-green, | Compsopogon, 8 CLASS II. § Multicellular or apparently so (A). § Unicellular, the cells often clustered or forming a colony (F). A, A. B. B. o: aoimmoo QO Chlorophyll diffused, not in patterns (B). Chlorophyll in spiral bands, axillary lamine, or twin, stellate nuclei (f). Filamentous (C). Not filamentous; articulate, prostrate, or net-like, often membra- nous, sometimes parasitic (M). Basal cell lobately divided, or with a terminal disk attaching the young plant (D). . Basal cell not lobate nor disciform (E). . Not branched; terminal cell sometimes setiform, C@dogonium, 10 . Not branched; no setz; strongly contracted at intervals, Schizomerts, 24a . Branched; with long terminal sete bulbous at base, Bulbochete, 11 . Branched; no setz; cells long, cylindrical . . . Sctadium, 46 . With branches (G). . Without branches, but sometimes with lateral processes (L). Plants filamentous (N). Plants not filamentous, sometimes curved or spiral, without root- lets (T). . Plants globose, small, on moist earth, with colorless rootlets (O). . Filaments erect (H). . Filaments prostrate or creeping, branched or not (*). s GENERA OF ALG&. 27 * Without dorsal spine;cell walls thick . . . . . Gongrosira, 21 power amee Spe 4 66 Ss , -» Aphanochele, 20 Pe teereea in firm jelly 2 Ce hetophora, 3 H. Not in a firm jelly (I). I. Filaments dichotomously or trichotomously branched, Microthamnion, 19 I. Filaments not dichotomously branched (J). J. Branches in lateral clusters; cells of main stem with a transverse, central chlorophyll band .... . . . WDraparnaldia, 16 J. Branches not in lateral clusters (K). K. Cell membrane usually thick, cells longer than broad; ultimate branches, much thinner than the primary, Cladophora, 23, or Pithophora, 24 K. Cell membrane very thin and hyaline; stem and branches barely separably in size and appearance Cg ee MACON, 47 K. Cell membrane thin; branches radiating, umbel-like, one-celled, Sciadium, 46 L. Distinctly many celled (P). L. Articulate (apparently multicellular) (Q). oe P: Q. . Articulate, branched, ee a little mass or a subdisciform layer et Bits oa OO a Coleachatle:¢ . Articulate, mr Gateate or OS a spiné on the back, Aphanochete, 20 . Expanded, leaf-like, smooth, formed of angular cells; always ad- herent ieee ge ea Ey aio. NS Membranaceous, vaBnlar: or ‘periutar. fixed only when young, Enteromorpha, 15 . Forming a green net, visible to the naked eye, Mydrodictyon, 41 . Forming a colony, plane; discoid or stellate, often perforated; cellspolygonal. .. . see oie Pasir ame, “40 . Filaments tufted, long, apiietinees ‘branched: . . Vaucherta, 30 . Rootlets much divided, descending from the cell base into the earth Up lgtan OA es OPAL SE Cells long; ehlorophy!l eaploaine hire: regularly placed vacuoles, . Spheroplea, 12 Cells long, filaments somewhat geniculate, without vacuoles (¢). Cells short, cylindrical or oblong; without vacuoles; filaments not contracted; attached when young. . . . Cylindrocapsa, 13 Cells short, broader than long, filaments contracted irregularly, Schizomerts, 24a Filaments simple, not laterally united, (R). 28 Q. GENERA OF ALG, Filaments 2 or more laterally united, or in flat narrow bands, Schizogonium, 26 R. With one row of cells, (S). S. Without lateral processes (except Ulothrix rivularts), strictly un- branched io sce. les 4. Chars ee onsera: 27 S. With short, irregular, ioteral processes, 1-3 or more celled, és y Rhizoclonium, 29 Parasitic, z. ¢. penetrating the membrane of the host (g). Not parasitic (*). * Cells united or clustered in families (U). * Cells scattered, independent or irregularly clustered (Z). Meats diddd re Mastigonema, 82 Filaments slowly motile . . . . . . . . . Oscillaria, 104 Filaments not motile (U). Filaments very slender, articulations more or less indistinct, Leptothrix, 106 Filaments cruciformly branched . .. . . Asterothrix, 106a Free-swimming, (¢. e. not adherent), (W). . Not free-smimming (X). Cells globose, or oblong when dividing, in a single, quadrate layer, bluish-green . . . . . . Merismopedia, 112 . Cells globose, sometimes in a tial colony, brownish or red, sometimes greenish . .. . . . . « Chlamydococcus, 38 Cells wedge-shaped, in radiating ities . Gomphospheria 115 . Cells filamentous or ring-like, spirally twisted, usually motile, Spirillum, 107 . Cells filiform, very minute, spirally twisted . , Spirulina, 108 Sheath present, gelatinous or jelly-like, often colorless (Y). Sheath none; cells spherical or angular by pressure, solitary or in small families Ronee tur re OE Te a . Aquatic (a). On moist timbers and earth; cells small, in globose bodies in a thin membrane. . Le gon eet OLY abe EE . Sheaths thin, not confluent: calle sabhlar: short, parasitic, Chamesiphon, 100 . Sheaths confluent, in a mucous stratum, or elongation or membra- nous (0). \ . Sheaths bladder-like, colorless; cells cylindric, ends rounded, Gleothece, 109 Sheaths confluent (c). . Sheaths membranous (/). Cells spherical or nearly so (@). Cells not spherical (e). GENERA OF ALGA, 33 . Tegument mucous, cells spherical or elongated, one pole color- less, notin families . . . Re ee 50 ia ee . Tegument mucous, with small eetucd families of innumerable eee. ieee ai ee a ee iy, OE, EF . Tegument soft, thick SOK aap eee eat id PS PE 120 . Tegument a mucous stratum, jactaiaiuie groups of two cells, these surrounded by another tegument. . . . . Gleocapsa, 119 . Tegument a mucous stratum; thallus spherical, hollow, cells many, small, onthe surface .. . . 4 1... -Calospherium, 113 . Tegument confluent, firm; cells longer than broad, Aphanothece, 110 . Tegument inconspicuous; cells elongate, cylindrical, Synechococcus, 111 . Tegument soon perforate, breaking into lobed forms; cells very small embedded «s+ 52... Sei. Y Clathrocystis, 118 . Cells globose, united in families, ‘hastened grape-like, Polycystis, 118 . Irregularly branched, often closely interwoven; stems and pri- mary branches:of the same thickness; often aerial, Chrvolepus, 22 . Cruciformly branched, ends acute; filaments articulate or not, Asterothrix, 106a . Somewhat dichotomously branched, apices rounded; floating and yellow, gr not yellow and coating wet rocks . . Calothrix, 81 . Filaments erect, laterally adherent, basally attached, forming layers on flat surfaces Pampa fe: kes . Filaments distinctly sheathed at hase. sheaths broad, often sac- cate, transversely plicate; heterocysts basal . . Gleotrichia, 84 . Filaments agglutinated by mucilage, and forming hemispherical or PUMNeTY POTMIS ee ee ee OIA SS Pai Li. Key to the Species of the Fresh-Water Alge. KEY TO THE SPECIES OF THE FRESH WATER ALG. I. LEMANEA., A. Filaments straight, 4-5 in. long, . . . . . . . fluviatilis, 52 A. i arcuately curved (a). @, 1-2 in. long, olive green, black withage.. . . . . torulosa 51 a. 5 in. long, violet in mass; regularly constricted . . catenata, 52 2. TUOMEYA. A. Much and irregularly branched; tufts 1-2 in. long . fluviatilis, 53 Me TaMONe a ee Oe grande Rg 3. BANGIA. Filaments about 1 in. long; in lax purple tufts . atro-purpurea, 55 4. BATRACHOSPERMUM. ~ A. Branches sometimes setigerous, internodes naked or nearly so; gelatinous. Variable .. . . . moniliforme, 56 A. Branches not setigerous, whorls inperiecdy developed, the branch- lets often impacted; color bluish green . . . . . vagum, 57 5. THOREA. Much branched, ramuli horizontal, long and short alternating, ramosissima, 58 6. CHANTRANSIA, A. Tufts 1 in. long; filaments with many straight branches; fertile DTAnCHGs Very Sacre. so eh ES ee eerogpora, - 5a A. Tufts 1-6 mm. long (a). a, Parasitic on other plants (3). a, Not parasitic (c). 6. Fresh water; bright violet, 2 mm. long; branches erect, ends obtuse, violacea, 59 > Ses SPECIES OF ALGA, 6. Marine; 1-4 mm. long in close fringe; branches rarely opposite, often Haity.. oss. oe Sa a, c. Terminal cells cuspidate or ; viliterses . . . . ermannit, 60 c a4 iM TORNGER |e WE ee a eee ee ; 7. HILDEBRANTIA. a. Fresh water; cells red; on river stones . . . . . rivularis, 61 a. Marine; cells spherical J hig eos POR Oey nce eee en ae 8. COMPSOPOGON, One species es eS Ea ee Se 9. COLEOCHATE. A. Filaments irregularly disposed, variable in form . trregularis, 65 A. ¢ radiating (a). a, Filaments not laterally adhering, forming small disks . soluta, 64 a. . adhering side to side, forming a disk (6). 6, Disk irregularly orbicular; cells subquadrangular . . scutata, 64 6. “ exactly orbicular, thin, flat, . . . . . . orbicularis, 64 6. “ orbicular, upper surface rounded, about 2 mm. high, pulvinata, 64 10. CEDOGONIUM. It is only possible to identify the sixty-three species of this genus when the plants are in fruit, the forms being arranged in groups ac- cording to some characteristic or peculiarity of the odspore (the seed or spore), and of the odgonium (the sac containing the odspore). It is therefore scarcely possible to devise an artificial key. § Male and female organs on the same plant (A). § Male and female organs on different plants (B). § Male organs of fructification not known (C), . Odspores globose or nearly so (D). «elliptical or ovate (2). Males short (dwarf), 1-celled (2). Males short (dwarf), 2—many-celled (J). Males filamentous, many-celled plants (gg). Odspores globose or nearly so ( ff). ‘ elliptical or ovoid (ss). . Odgonia globose or nearly so (a). ‘¢ elliptical or ovoid (2). ee opening by a pore (0). ““ ie ee 3 lid (/). . Pore median; veg. cells 4-6 times as long as wide . cryptoporum, 70 fT Sere rer re RHP XN RAHA DO & SREMXENSHSNRNKRNHBDBS SREYYYRHKPSDDWMS . Odgonia without vertical constrictions (/). . Odspore globose or nearly so (m). “6 subellipsoid (a). . Diameter veg. cells 15-174, 4 times constricted . undulatum, i" «28-384, not constricted . . cataractum, . «from 9-15 44 (72). . Odspore depressed-globose ...-. .. . . . decipiens, xt exactly globose . . . prig oo LN resehougt, Dwarf males adhering to all parts of the female . mudltispora, ss “ onornearthe odgonia . . . . . cyathigerum, . Odspore membrane smooth (g). ‘6 é beset with bristles (cc). . Odspores globose or nearly so (7). m ellipsoid or ovate (zw). : USecns opening by a lid (s). “ ‘“¢ a pore (?). ) Terminal cen Toupge 26). eee ek oe Beene, apie miate. © 345<. . . . macrandrium, Pore in the middle or a little above i: ““ above the middle (z). . Veg. cells 4.5-6 times aslong as broad... . flavescens, “ “ 2-4 ZT: COE ee “6 ““ PONS See Braunit, SPECIES OF ALG&. 39 . Pore above the middle (c). Odspore not quite filling the odgonium (2). Odspore entirely filling the odgonium (e). . Veg. cells 3-5 times aslong as wide... . . . obsoletum, 71 - ee RTA on ee oe . . . . plustospermum, 72 i BAA er a ee . . . . polymorphum, 73 Veg. cells 4-5 timesaslongaswide ..... . . fragile, 71 ““ ‘“ 2-4 CO he 6k rT: “ gee Nee . Zig-Zag, 71 . Odspores subglobose, filling the o6gonia . . . autumnale, 73 . Odspores obovate, not entirely filling the odgonia (g). . Veg. cells 4-6 times aslongas wide... . . ... wernale, 72 Dee COG Soh ts oy i pis + 3 eraspum, 72 Odgonia single, opening by a seagate pore; GAcouce globose not filling the odgonium : pg MPO iee F2 Odgonia opening by a pore Shave the ‘uaddie ( aN, Odgonia . PM en) i pot SU i, hs SAT Ph Veg. cells 3-7 times as long as wide ... . . paludosum, 74 cc < 2-5 é< “ec “ ‘<“ “ ; : f : ; CraSSUM, 74 . Odgonia with 7-12 vertical Gonebrictions . . . platygynum, 75 40 : SPECIES OF ALGZ. v. Diameter of veg. cells 13-14u¢ . ... . . . pyriforme, 95 v. ef 6 ES OO Oe BOE SE a mee ae v. $ FE BONE ee ee ee w. Odgonia opening by a pore (2). WwW. “é “6 ce Ge lid (aa). x. Pore above the middle (9). x: below ‘“‘ odspore with 4 spiral ridges . Huntit, 85 y. Odspore longitudinally costate . . . . . . . Wolleanum, 82 z. Diameter of odgonia 45-504 by 60-754 . . . #£Boriseanum, 81 Z. prac: 3 a 70-8314 by go-1o5fu@iwsi(‘(‘(wsst«Cf:SCCOnCatenatum, 81 Z. va it tl 29-32 by 33-38u . . . . + sexangulare, 82 aa. Odspore longitudinally costate, filling the odgonium, acrosporum, 83 OA. eK not ef 5 (40). 66. Terminal cell setiform ) oa a a a a eee oe bb. Jy “not setiform . . . . . . obtruncatum, 84 cc. Odspores ellipsoid odgonia opening ay a pore in the middle, Lystrix, 87 ce. “i globose (dd). dd, Odgonia opening by a pore at the middle . echinospermum, 86 ad. 1 SE SS DONS Ce ake ceed. ee. Spines of odspores conical (//). Jf. Diameter of veg. cells 2.5—5 times as long as wide . stellatum, 85 Tt. es ae “ 1-2 times as long as wide . Donnellit, 85 Tae " MAUS 6 64 IO OM OS en rr, oe gg. Odgonia not or scarcely swollen (4%). eed manifestly swollen (77). hh, Diameter of odspore 22-52 by 38-624 =. w:C(«wsC‘(“«‘€;:sS«CCaptlaae, 87 ie a ro i: 47-49 by 50-664 . . . . sStagnale, $7 z. Odspores globose or subglobose (7). ii i ellipsoid or oval (00). D- i dies opening by a pore (£2). Th " ‘“ “ lid; odspore globose . . Pringshetmit, ga kk. Pore in the middle; tuceabbaie of veg. cells and of o6gonia spirally PUnClate 4.525; . . puncto-striatum, go kk. Pore in the middle; mena ‘en wiihictake . 6 WAP CSCERS ABO kk. Pore above the middle (ZZ). 47. Odspore filling or barely filling the o6gonia (mm). Zi. Odspores not filling the odgonia (nz). mm. Odspore globose, filling the o6gonium, diameter 25-29 by 24-30) _ Franklinianum, 89 SPECIES OF ALG, 41 mm. Odspore sub-globose, ais filling the odgonia, diameter 42-soyu by 46-56 : carbonicum, go nn. Diameter of odspore aaa I by ieese fie capilliforme, 88 nn. 2 4 4 51-54 by 73-85 pachyandrum, 89 un. Diameter of odspore 42-60 by 42-6op . cardiacum, 90 oo. Diameter of odgonia 40-454 by 80-1004. Boscit, 91 00. . sf 4 63-75 by 105-120 . | Landbaeehe. gi 00. 3 - s 7o-85 4 by 130-160p . rivulare, 92 00. is os = go—1ooft by 130-150 . _ var, major, 92 pp. Diameter of veg. cells less than 164 (99). pp. és rit “ 16 or more (77). gg. Diameter of odgonia 17-18 by 20h . adelicatulum, 93 99. i “ «24-28 by 28-35 . montiliforme, 93 99. DEN OF ares MD Sard i Londiense, 94 99. A . “24-25 by 26-28 hexagonum, 94 rr. Diameter of odgonia 36-40 by 44-45) . . fonticola, 93 rT. és . as 61-754 by 68-80p . princeps, 93 ss. Diameter of veg. cells less than 25 (¢t). SS. 3 woe - 254 or more (wu). tt, Diameter of odspore 35-36 by 45-48 yu pyriforme, 95 tt. ey fi ee 15-16 by 17-19 longatum, 95 wu. Diameter of odspore 54-654 by 75-103) gigateum, 94 uu. - fs “45-50 by 68-724 grande, 95 Il. BULBOCHATE. In reference to the sixteen species forming this genus, the remarks made in connection with @dogonium might be repeated. The spe- cies can be identified only when in fruit; to make an artificial key is therefore almost an impossibility. Replying to a personal inquiry in regard to the division of the species into moncecious and dicecious groups, with the presence of dwarf males on or near the odgonia of all the so-called dicecious forms, Mr. Wolle writes: ‘These male forms are not developed when they appear; they are supposed to have been formed in dis- tinct cells known as androsporangia, and when they have developed and escaped, they float about and then attach themselves on, at or near the oédgonium, but they are not necessarily a part of the plant on which they may be seated.” 42 SPECIES OF ALG, § Odgonia globose or nearly so (A). § ‘¢ ellipsoid or nearly so; odspores longitudinally crenulate- costate (F). A. Dwarf males 1 celled, on or near the odgonia, elachistandra, 97 A « “2 celleds¢B). B. Basal part (stem) of dwarf males, shorter than the spermogonia (C). B “ mad iT) “c “ “é longer than the spermogonia; oOspore warty .. . je 2 ag Coe ee arenes. eee C. Diameter of veg. cells 204 or ‘ean (D). | ea Ls eee “ more than 20; odspore granulate, setigera, 98 D. Odgonia without terminal seta. . . . . . intermedia, 97 D. ‘¢ with terminal seta, or beneath a eat cell (E). E. Dissepiment supporting odgonia in the middle of cell or. slightly below ara digg tly TERE: AE E. Dissepiment ahave ete sniddie ag the eet . . . . polyandra, 98 E. pe below the middie ofl. So rebisonas, a6 F, Moneecious (G). F. Dicecious (H). G. Diameter of odgonia 27-354 by 46-564 . . . . mirabilis, 100 G. re sf i 20-25 by 33-364 . . . . . mana, 100 H. Dissepiment of cells supporting odgonia obsolete (1). H. iti ‘74 “é 73 “cc present (J. ) I, Diameter veg. cells 12-54, length less than diameter, pygma@a, 101 J. Diameter veg. cells 16 or less (K). i . 2 ‘* more than 16y (L). K. Veg. cells 1-1.5 times as long as wide . . . . subsimplex, 101 kK ‘c“ << 2-3. 5 ““ “<“ “ “cc ““ repanda, 102 Bes ies Oe ee Si Ss RN etree rhadinospora, 103 L. Veg. cells 2.5-4.5 times as long as wide .. . . émsignis, 101 RD ree eres ee ce ign ey eee ‘san minor, 10% A ae yj Meera ne ron pne) PouNE Mey ee a Se 12, SPHAZROPLEA. Cells 8-10-20 times as long as broad, chlorophyllose rings 20-30 in Carb ey ee a ie 13. CYLINDROCAPSA, nds of Cells cleat and pearly a see amena, 105 Ends of cells notclear . . . . . .. . 4 +. geminella, 104 SPECIES OF ALG#, 43 14. PRASIOLA. Tufts 2-6 mm. high and broad, cells 4-6 in diameter, crispa, 106 § a, a, “« 1-3 inches long and broad, cells 6-8 ‘“ «Mexicana, 107 I5. ENTEROMORPHA, In brackish water (a). Fronds simple, elongate; cells 3-5-6 angled . . intestinalis, 107 Ny COMPLOsheG, LNDUIAE fo 2 ak a, OPES, TOT 16. DRAPARNALDIA, A. Every mature joint with 2-4 clusters of branches, Ravenellii, 110 A. Branches less abundant, not at every joint (qa). a, a. Or Nw Ww & Q MH RS NS KAKA Se So S. Branches opposite, their stems thick, short, tapering to a point or ae ras Boa a EOS en a ia aay, | AOS EO Branches opposite or alternate . . glomerata, 108, plumosa, 109 I7. STIGEOCLONIUM. In warm or hot water; 1-2 in. long, much branched, apex a bristle, thermale, 111 Not in water of high temperature (a). . Branches ending in a hair or bristle (4). . Branches not ending in a hair or bristle (A). . Bristle a colorless, apparently hollow prolongation (c). . Bristle a delicate, filamentous, hair-like extension (¢). . Cells nearly cylindrical, equal or twice as long as broad, protensum, 112 . Parasitic, dwarf,2mm.long;in mucus . . . . . radians, 115 . Not parasitic (e). Diameter of largest cells 20m or less (/). ws " “« 25-40“ . . . | Mudiusculum, 113 . Diameter of filaments 11-20; basal cells 6-8 times longer than MG eas Os wae 2 SOCAN UI TEA . Diameter of filaments 10-16 (4g). . Branches opposite on cells smaller and more oval than the others, flagelliferum, 112 . Branch-bearing cells not smaller (A). . Upper branches clustered, moniliform . . . . fastigiatum, 114 ‘ not moniliform (7). . Two or more inches long, branches mostly opposite, fasciculare, 114 . Two to ro mm. long, simple to near the end, there branches Ciistered i a ee gi a oe ioe pare £8 44 SPECIES OF ALG, » 7 Length 4-50 mm.; somewhat branched, branches simple, tenue, 110 , ‘¢ - 2-3 mm.; branches alternate, ends obtuse, . mnanum, 112 R. «« 12 mm.; branches mostly opposite, ends acute, | amenum, 113 18. CHATOPHORA., Jelly mass globose or nearly so (a). Jelly mass elongated to 3 inches, margins tattered, forming ragged Oranches 00 Ses . . . endiviefolia, 117 § Jelly mass orbicular, small: Hlameuts heir: pointed . Jongipila, 118 a, Branches more or less moniliform; jelly mass 2 mm. diameter, montilifera, 118 SR LI a. Branches not moniliform, radiating (0). 6. Jelly mass smooth (c). b. “ tuberculose, ends of branches rarely hair-like, tuberculosa, 116 c. Diameter of branchlets 6; 1.5-3 times longer . jpistformis, 116 ¢. ey “ branches 7-10; 1-1.5 “ OO i anges a ee 19. MICROTHAMNION. But one species. io Pe ree 20. APHANOCHAETE. § Parasitic (a). § Not parasitic (4). a, Cells globose, a bristle on the back ey iy POP OMS, IO a. Cells various, elongate, curved, worm-like, sibs iotsae: vermiculoides, 119 6. Cells globose, or subpyritform, a seta on the back or apex; in PCB oe a el eee el ee aa 21. GONGROSIRA. CONG SPECIES ui, Ae ae gee a ere es ee 20. CHROOLEPUS. a. Cells globose, broadly elliptic or ovate (0). a. Cells more or less elongate (c). 6. Dull or bright red; diameter 14-224 = wg = ws s=C«wsSCiwssts em brrinum, 123 6.-Ash color when dry; diameter 20-25 . . . . moniliforme, 123 é. Diameter of cells more than 25-40; branches rather dichotomous, Lolithus, 122 c; 25 « — less than 25 (a). SPECIES OF ALG#, 45 . Stratum tawny red, drying greenish; diameter of filaments 9-14, odoratus, 122 ; Stratum orange-red, drying yellow; diameter of filaments 7-9, lichentcolus, 122 . Stratum red or orange, soft, silky; much branched; diameter of Re RSS aiitg ae a es ae ee MT US VST 23. CLADOPHORA. Chlorophyll diffused, not in spirals (a). ¢ disposed to be net-like or in lax spirals (¢). . Cell membrane smooth (4). " se plicate-striate; contents of young slightly spiral, crispata, 126 6, Cells more or less swollen; irregularly branched; variable, Jracta, 124 6. Cells not noticeably swollen (c). c. Diameter of branches 28-40 . . . . . . .. oligoclona, 126 é a ¥ abouti5u St . . . &lotowiana, 126 d. Branches united (connate) at base; cells 5-8 times as long as broad, cantlicularis, 117 d. Branches not connate at base (e). e. Filaments not radiating from common centre, cells smooth (/). é. ‘ 5 e cell membrane plicate . flavescens, 128 é. ei radiating from a common centre, much branched, | agagropila, 124 jf. Diameter of stems 60-100u . . ... . . . glomerata, 127 Fe ss Pe SAO Rae my er Coe LN es eee eae 24. PITHOPHORA. me Fertile stem Goiethick 9s 3 dagonna, 1136 An OPO OIC a ee a ee aequalis, 13% Pe “59 thick ART NE ard oh era Fs, ef 24a. SCHIZOMERIS. COR Sette i ee eg a es eereeing, FEC £, 25. ULOTHRIX. § Length of cells equal to width (a). eo “« “greater than width (e). Bek «« «less than width (A). a. Color dark, light or bright green (0). a. Color yellowish green (@). 46 SPECIES OF ALG&, 4, Filaments dividing longitudinally; mucous; 4 in. long, floating, Lenormandt, 138 b. Y not dividing longitudinally (c). ¢. Joints often constricted; diameter of cells g-I0ofk . rivularis, 136 c. Joints not constricted; aquatic; variable; diameter of cells 5—soy, subtilis, 135 Bs 3 * = filaments tortuous, very long, muralis, 137 Bey é: “ On damp walls, pavements, exposed rocks, etc., (*) *. Cells 6-7 in diameter; on walls, pavements, etc. . mitens, 137 Ms EOS Mee si cell contents usually at one side; on ex- — posed TOCKS, ETC Fea PE eee es d. Cells 12-14 in diameter. . . . @gualis, 134 d, Cells about 7 in diameter; on moist ley or tose . . Varta, 138 e. Green, cell contents usually on one side, hemispherical, flaccida, 137 e. Green, dark, bright or pale; contents diffused (/). f. Joints often constricted; cells g-1oy indiameter . rivularis, 136 f. ©“ not constricted; filaments attached, 17-25 in diameter, tenuls, 134 PRE y ’ filaments not attached (g). g. In brackish water, filaments 1-3 in. long, 1o-30y dia., Younganum, 135 g. Infresh water. . fy EE, hae hk. Filaments dividing lonpitiiinally, 3 4 in. en Aspley: Lenormandt, 138 h. not dividing (2). t. Joints often constricted, cells g-1oy in dia.. . . rtvularis, 136 7, not constricted in sterile filaments (7). j. Filaments roy or more in diameter (£). y less than roy in diameter (m), k. In brackish water; filaments 20-30 in dia. . Younganum, 135 Rk. In brackish and in fresh water; filaments 38—soy in dia., Spectosa, 135 Rk, In fresh water only or on the ground (2). 7, On shaded ground; flexuous, interwoven. . . . .parietina, 138 7, Aquatic; filaments 1o-t2uin dia. . . . . . . oscillarina, 137 . ¥: 6). Ten 4Op te aes wig Naan: } Ma ley m. Bright, grass green; filaments ‘eebacien pe long, interwoven, muralis, 137 m. Yellowish green, mucous; cells shorter than wide . compacta, 136 SPECIES OF ALG&, 47 26. SCHIZOGONIUM. One species; found on moist cliffs, Colorado . . . . murale, 139 . ' 27 CONFERVA. § In brackish water; diameter 12-14) PIRES, var. salina, 141 § In fresh water (A). A. Diameter of filament from 3-5 (a). A “ ie “from 6-124 (4). A. Peer s from 14-25 (g). a. Very pale; cells 1%-2-3 times as long aswide . /enerrima, 143 a. 6c 66 ““c 4-9 “cc ce “ eh ay Se. 2 eee eee ee. “ 58-65 “ at ‘ . . . orthospira, 218 Jf. Filaments of two dinnuetere: one saa ithe other 80p, inequalis, 218 Tf, ¢ “ same diameter, 150-1560). FN ere, 280 ff ‘6 io ah ‘< 102-110. . ._~—s Set formis, 219 ef, “ “ek é r1I8—125m@ Fo Samia, 218 Sf. ‘6 “Ke as 54-62u . . . . . mazjuscula, 221 gg. Cells 4-10 times as long as broad, 24-27. diam. . mitrabile, 211 PO ae eae ee ee “ 25-30udiam. . J/ongata, 214 Il.—KEY TO FERTILE FILAMENTS OF SPIROGYRA. For method of determining the number of bands in the cell, see the Key to the sterile filaments of Spirogyra, p. 56 § Cell membrane folded in at the ends (a). aia not folded in at the ends (). a. cBro eng band single, rarely double (0). a. «two or more (A). 6. Membrane of spore smooth (c). b. " “« “punctate (2g). c. Spores elliptical or fusiform (dz). c. “ ovate or oval (e). | @. Spore 24-30" by 50-58u . «www wtwttstCi«w#stSsChMUISSIMA, 207 d. © 30-36 in dia:, twice as tong =... ok. . . efiefa, 207 d. “ 27-32in dia., 2-4 timesaslong . . . . . guadrata, 208 ¢. Spore-bearing cells fated (/). es ten 28 “« slightly dilated; spore 26-30, in dia., Weberi, 208 fF. Spores 36m in diam., 2-3 times as long as wide . Spreetana, 208 Sf. “ 30-36 in tok. 2-254 Ms ig APO ane, 200 SN Nw wD. DDS ee ee sess SPECIES OF ALG. 59 . Mature spores yellow or orange, 40-42 in dia. 2-3 times longer, calospora, 209 : Seey Meshing cells not swollen, spores twice as long as wide, Flantzschit, 211 . Spore-bearing cells swollen (7). Mature spore yellow, 42-48u long . .. . . . Hassallit, 210 i: “ brown, elliptic, 284in diameter . . . imsignis, 210 One chlorophyll band in each cell (£). Pear more Sc Fy a Fe fd). . Membrane of spore smooth (2). Hf so Sct ate: (ay. Mature spore yellow (7). " “not yellow (/). . Spore cell slightly swollen on one side, spore oval,. twice as one aswide =. |. Se AL gee ON gk eg PRIN EE . Spore cell not swollen ta . Spore cell swollen (o). . Filaments 20-224in diameter . . . . . . . communis, 213 + 25-30) “ ae DR Ble gta uae tre ROE, Ve . Filaments 24-26y in Hamers hee ak tit Me Ene da es Sy Meek - 40-434 “ e: Se cade RAINE 2 ak Spore-bearing cell decidedly bwolien ie : “ not or slightly swollen (7). " “ *¢ swollen on one side only . . . varians, 212 Filaments 25 2) Camicter ea nial, 282 s 13-20 “ és Sl DESCERS.” 25% . Filaments more than 30pm in diantcter 4). “ 30 or less in diameter (Z). . Diameter of filaments 33-40; spores polymorphous, gwinina, 213 ‘s yy 3 40-554; spores polymorphous, fusco-atra, 215 - 2 48-75 4; spores broadly elliptic, condensatla, 215 . Diameter of filaments 25-304; spore twice as long as wide, ; _ longata, 214 ‘6 46 “ 22-254; spore slightly longer than wide, subsalsa, 212 . Mature spore yellow, spore cell much inflated, . . pwunctata, 215 . Spirals 2 (w). ee Lee). 60 SPECIES OF ALG. Sey eines C8 & v. ‘ 6or more (7). w. Spore-bearing cells inflated, spore elliptic or ovate, elongata, 221 ee eR . “not or only slightly swollen (x). x. Filaments 32-40 indiam. . . .. ... +: . . @ecimina, 216 m8 20 It 15 Io I2 23 | KEY TO THE SPECIES OF THE DESMIDS. 1. GONATOZYGON. . Cells swollen at base, with 6 longitudinal lines of short sete, sex-spiniferum.,** . Cells not swollen at base (a). . With hair-like spines clothing the surface . . . . fpélosum, 22 . Without hair-like spines; surface minutely roughened, asperum, 22 2. GENICULARIA,* FIG. 2. . Cells 3% to 6 times longer than broad; granules in spirals, Americana.* . Cells ro—-12 times longer than broad; granules scattered, spirotenia.* 3. -HYALOTHECA. FIG. 3. . Cells slightly constricted, length % the width, sides not notched, disstliens, 22 19 a oa i! “« sides arched, cen- traity rotcheds.)7::..% pe ale AS aoe 2. Cells slightly concave, iehotn twice the wads . . undulata, 23 . Cells not constricted, margins straight; sheath wide, mucosa, 23 4 4 “ Sheath absent, dubia, 24 4. BAMBUSINA. FIG. 4. . Cells hub-shaped, somewhat longer than broad . SArebissonit, 24 . Cells hub-shaped, nearly twice as long as broad’. gractlescens.** 3. Cells subcylindrical, 4 times longer than broad . delicatissima, 25 *Journ. R. Micr. Soc., Dec., 1885. #*F W. Alge, p. 21. SPECIES OF DESMIDS. 81 5. LrEprozosMa. An immature form of Desmidium, 6. S AY LS Q& Qe NNR 6. DerEsmipium. FIG. 6. p ieads sieath present. 23. eylendricum, 25 . Mucous sheath absent (a). . Cells united by their entire end margins (4). . Cells united by the outer portions of the ends (¢). . Cells nearly twice aslongas broad . .. . . . longatum, 26 Cells less than twice as long as broad (c). | Cells in side view quadrate . guadratum, 26; guadrangulatum, 27 Cells in side view triangular’. . .°. ; .°... . Swartstt, 26 . Borders crenate or undulate . . aptogonium, 27; diagonum, 159 . Borders straight, filament twisted . . ... . . Batleyi, 27 7. PuHyMatTopocis. Fic. 7. Re ea el See 2 Aordsbeinanum: 28 — KASS SsSRnrn- ES 8. SPHAROZOSMA. Fic. 8. . Cells twice éroader than long, lobes not constricted (a). “ Ms “ “ lobes constricted near the end, constrictum,** . Cells twice /onger than broad, in sheath or not . excavatum, 29 . Cells less than twice longer than broad (6). . Cells closely approximate, ends rounded . . . . pulchrum, 29 As a eh ends truncate, concave, rectangulare, 31 Cells more or less remote, ends rounded . . . vertebratum, 30 Ends pointed; semi-cells remote; sinus deep, wide, *monzliforme.** Ends rounded, spinous; cells slightly constricted, spznulosum, 31 Ends rounded, not spinous; cells deeply constricted, fliforme, 29 Pree srmcate, CONCAVE) 6 re te oe eWeek, 30 minds truncate, mot concave © ...:0-. 0.0 soe. | pulchellum*®* 9. ONYCHONEMA.* FIG. 9. . Cells with spine-like projecting ends ... .-. . = serratum, 30 . Cells without spine-like ends . . . . . . . Wordstedtianum.+ 10. MESOTANIUM.. FIG. 10. . Cells cylindrical (a). Cells oval or elliptical, about twice longer than wide, in mucus on WU Oe gore OR ce OCCU, 32 *Mr. Wolle joins this to Spherozosma. + Journ. R. Micr. Sec., Dec., 1885. **F —w, Algee, .pp 23 ef seq. 82 a. a, QR 2 - A R Rw N Raney Peers NX & SPECIES OF DESMIDS. Mucous masses floating; cells 2-2% times longer than wide, Braunit, 3% Mucous masses mingled with filamentous algze; cells 3-4 times longer than wide Endlicherianum, 32 . Mucous masses on wet rocks and? mosses; cells 2-3 times longer than: witke 3) eA Ot a ae ee II. SPIROTANIA, FIG. II . Spiral band single (a). ~ Spiral bands more than one... 350 ck, Whee eee as _ Cell 8 to 10 times longer than broad . . . . . condensata, 33 . Cell4timeslongerthan broad . ... . . . O6ryophila, 33 I2.:. PENIUM:, ... FIG. 22. . Chlorophyll interrupted by 1 central transverse band (a). ‘“ ‘6 “‘ 3 transverse bands; cytioderm smooth, interruptum, 35 ée &< 6 66 ‘< “ cytioderm spirally Striate © 2c ies Fey al a SPAT OSLO DEGTE” . Chlorophyll sonceatteieds into 2 or more nuclei; in mucus, crassa, 37 a diffused (0). . Endstruncate, square .. . ny nee at A TREE SR . Ends not truncate; cells slightly worethioued: sides straight, minutum, 35 ‘“ ‘ ‘6 ‘“ ‘< “ sides convex, cructferum.** 6 x as cell not ae 3-5 times longer than WIGE borane: Bee ee Cad ee . Ends not truncate; Celi not sonata: gel times longer than wide: if@2%2 SS SOAS | ROT a Gs rc or . Cytioderm smooth (c). ee with pearly granules in longitudinal rows, margaritaceum, 34 Cells in mucus, diameter 34, to z4, in. (63-83) 3 to 4 times as, long aswide .... . . . . oblongum, 34 i) REA OE Ce aa iphes as ivide i Hiile 1204 MP ESTPE,” 29 «< “more than 3 timesas long as wide . Arebissonii, 36 Cells not in mucus; each semi-cell a truncated cone . stumidum.** . Cells oblong, often slightly constricted . . . . Jlamellosum, 34 . Cells subcylindrical, in families of various sizes intermingled, polymorphum, 36 . Cells subcylindrical, not in families . . . . i fernere, 26 . Cells broadly fusiform, 4-5 times longer bed wide: navicula, 36 **J. R.M.S., Feb. ’89. S S Nn ON ey sais Pe SY & Q&RQN AO OR SPECIES OF DESMIDS. 83. 13. CLOSTERIUM. FIG. 13, @, d,c¢. Ends not or but slightly produced (1). Ends produced into long, often setiform, beaks (2). . Cells straight or slightly curved; ends slightly tapering (a). a as se 5 if dorsum convex, ventrum nearly straight (¢). . Cells conspicuously curved; ventrum concave, with a central in- flation (2). . Cells conspicuously curved; ventrum without an inflation (7). . Body margins equally convex; beaks longer than body, cetaceum, 47 ets « “beaks % as long as the body, pronum,.** . Body margins not equally convex (mm). . Length 5-12 times the width (4). . Length more than 12, less than 20 times the width (e). . Length 20 times or more than the width (/). . Ends suddenly contracted; cell fusiform, 5 times longer than wide, SMM aig . . . masutum, 41 Ends not contracted, Hut Gerag, Site: ehorophyi bands sev- eral grandest foW 2. i. a. Sanceolafum. 39 . Ends not contracted, rounded (c). yi : truncate (2). Cell slightly curved, small, 6-12 times longer than wide, smooth, acutum, 44 “ ts bs 5-10 longer than wide, smooth, odtusum, 38 Cell nearly straight, decussately striate . . . . decussatum, 39 bs x ** smooth, apices obtusely rounded, addymotocum.* . Cell slightly curved, 6-12 times pees than wide, striate; vacuole Gistinct i 4 ei . . . . @tdymotocum, 39 Cytioderm with 4-5 eristeudintal strie, often with 2 or 3 transverse bands and decussating strie . . . . . angustatum, 40, Cytioderm striate; ends slightly incurved; globules about 20 in each, eemi-cell axillary 5°; 2. Seika 2 COMORES ee Cytioderm striate; ends truncate, corners rounded; with three Seeererse Butaies OS Pe ee. sabdircthan > Diameter 2 orless;smooth .. . . . gracillima-levissima.* Diameter 3-4; slightly curved, smooth, or with 1-4 trans. striz, subtile, 158 " RE ee MICE Ge yg epee ee ye Se RU Oey AO S 10-1644 (4). *F —w. Alge. p. 23 ef seg. **J. R. M.S., Feb. 89. 84 ne f. ee q q « & i | SPECIES OF DESMIDS. Diameter 18; about 30 times as long as wide, smooth, ends FOuNdEg SA eee ae ee . 20-21; striated: éndé raioded” vee anh pheonguam.® = 364; about 20 times as long as wide, smooth, ends not curves "5. é Rees Acc Surface smooth; slightly Sanevins froin the middie . strigosum, 42 ne longitudinally striate only; diam. 11-124, juncidum, 38 ‘ ‘“ a with 1-4 trans. striz; diam. 12-13), mactlentum, 38 . Ends inclined upward at a dorsal depression; ventrum slightly con- cave; strie fine, numerous .. . i PUNE |r ee gt . Ends suddenly contracted to a narrow palit: cell slightly curved, attenuatum, 41 . Ends not suddenly contracted (A). . Cytioderm deeply striate; distinctly Reaniiets or areolate, arcolatum, 43 tf more or less distinctly striate; cell linear-fusiform, 15-24 times longer than wide .. . . . @cerosum, 41 . Cytioderm 9-12 longitudinally costate; cell paper aa, 4-5 times longer thanwide ... . . . . subcostatum,* . Cytioderm more or less diguisenae striae: may semi-lunar, 5-6 times longer than wide... . Shvhe ey Sa, . Diameter 51, to sf, in. (75-1104); eyodern smooth, Ehrenbergii, 45 Diameter ;4, to zh in. (40-60); globules a single row, moniliferum, 45; Letbleinit, 46 Ane ay “ cell curved, rapidly tapering into narrow, somewhat peas fh) ends; cell 6-8 times longerthan wide ... . oe Sco tee ee Diameter =}, to x4, in. (35-454); 2 or 3 Sutures in the middle; about 20 times longerthan wide. . . . . . .° Delpontii.* Cytioderm with many distinct striz; ee 6-16 times the width; vacuole large . . . . . . Sstrtolatum, 42 Cytioderm with fine striz; length 12— ioe fies the width; vacuole SMaa oe. . . decorum, 43 Cytioderm with 5-8 Manet striz; ‘derh 6-8 times the width; vacuole large .. . .. costatum, 42 Cytioderm smooth; cell crescent- shaped: often ‘subabsbicincutar (2). a . cell not conspicuously crescent-shaped (/). . Ends separated 7-10 times the diameter; width ;,4,5 to +> in. (EORO JE) i oe Fie OSE eee ea ye ir *F-w. Algz, p. 23. SPECIES OF DESMIDS, 85 k, Ends separated 7-10 times the i, existe to zq55«Cin. (25-28) 2 +. . . acuminatum, 44 k. Cell 6 to 8 times longer than wide; ‘ends. obtuse; width =, in. (14) Ea bod ite Seen fo 7g sae © k. Cell 6 to 8 times longer than wide, ends obtuse; width 5,4, in. eat . . parvulum, 45 k. Cell 8 to 12 a3 longer han wide, aes semicircular, ends sharp; width 57, to,755 in.(8-10m) . . . , . Venus, 44 7, Cell stout, ends taste rounded; width ,1, to z 45 _*=in. (25-30), cucumts, 40 m. Beaks slender, nearly as Jong as the body, ends obtuse, curved, : Kuetzingit, 47 m, Beaks thin, % as long as the fusiform body . . rostratum, 46 I4. DOCIDIUM. . FIG. 14. § Suture a projecting or conspicuous rim (a), § Suture not projecting (2). a. Cytioderm hirsute; semi-cell with 3 or 4 undulations, spznosnm, 51 a. Cytioderm not hirsute (4). 6, End dentate or crenate; semi-cell with 1 basal inflation (c). A og fc “ “« semi-cell with 4 alias inflations; teeth onthe margin. . . . . constrictum, 50 6, End dentate or crenate; semi- cell wab 3 or more inflations; teeth below themargin . . . . . nodulosum.*™ 6. End truncate or rounded; semi- cat with: 1 or 2 basal inflations, Trabecula, 48; truncatum, 48 4 ey ‘ ie . semi-cell undulate to the contracted end, crenulatum, 47 he rs i . i 1-2 undulate at base only, Archerit.* c. End with 1 tooth oneachangle ... .. . . Slowtowit, 49 ¢c. End crenulate with tubercles . . . . . ¢coronatum, 49 d. Cytioderm hirsute; base of semi-cell slightly faflatodk hirsutum, 51% d. Cytioderm not teats (e). e. End dentate or crenate (/). e. End not dentate nor crenate (7). J. Semi-cell with 4 or more inflations (4). if . with whorls of quadrangular prominences, verrucosum, 52 7. ds with 20 or more constrictions . . . . . costatum, 53 y: “with 1 inflation (g). | *F.-w. Alge, p. 25. a > % OF SPECIES OF DESMIDS. . End with numerous pearly teeth or beads . . coronulatum, 49 . End with prominent teeth, about 3 in view . . ¢ridentulnm, 52 . End with 3-5 minute tubercles; semi-cell with 4 or more undu- lations . . Mee rem TE . End with toothed annie (i). Semi-cell with 4 prominent nodes; 8 to to tees longer than wide, nodosum, 50 as “« 4 constrictions; ro—12 times longer than wide, breve, 51 ¥ “ 8 constrictions; 20-24 times longer than wide, sinuosum, 5% Semi-cell with 1 basal inflation (2). undulate to near the end (Z). 43 with 4 larger and smaller undulations; cells 12 times as long as wide (m). : Semi-cells not or slightly undulate; densely granulate, Jdreve, 158 . Cytioderm densely, irregularly punctate . . . . clavatum, 48 pf smooth; ends truncately rounded, margins often wavy, ~ Baculum, 49; rectum.* ae 3 ends round; cell minute; diam. 7-12), minutum, 52 a : 2 " cell, large; diam. ends soy, Woodit.* Diameter ;,)5 in. (25/4); about 20 times longer than wide, repandum, 50 Diameter 7755 tO zyp in. (13-164); 15-20 times longer than wide, adilatatum, 50 . Diameter 3355 tO sy/g5 in. (10-124); 18-20 times longer than wide, undulatum, 51% m. Surface punctate; suture none; ends roundly truncate, Georgicum.* I5. TRIPLOCERAS. FIG. 15. (Mr. Wolle unites this with Docidium, 14.) 1. Tooth-like prominences oblong . . . . . .. vwerticillatum, 53 S § $6 acute Bia fig cing ig eg Hise tae ad i 52g ios 7 rrr 16. CALOCYLINDRUS, FIG. 16, Chlorophyll homogeneous (1). " divided or scattered in each semi-cell (2). *F.-w. Algz, p. 25 ef seq. A RAR YAR ® so SS 8 Y&R RNR HN HH HH HOD SPECIES OF DESMIDS. 87 . Length twice the width or less; cytioderm punctate or granulate (a). 6c ‘c ‘“ ‘< 7a sade cytioderm smooth (4). és ‘ ‘é rT “Ok cytioderm with 5-7 coste, costatus, 56 . Length 2% or 3 times the width; cytioderm punctate (c). “« 4to 6 times the width; cytioderm smooth . minutus, 54 . Cell twice or more longer than wide; cytioderm punctate, pseudoconnatus, 55 “ ‘ “ “ 6 “ cytioderm smooth, Thwaitsi, 56 . Semi-cell subquadrate . . Pena age ors); i One | ‘§ cylindrical, rounded; Raeeeelion slight . cucurbita, 54 eS . constriction wide, shallow . . connatus, 55 " sub-semicircular; constriction slight; ends rounded or somewhat truncate; end view circular . . . Cordanum, * . Cell somewhat fusiform, ends subconically rounded . curtus, 54 . Cell subcylindrical,ends broadly rounded . . . dadplospora, 56 . Cell subcylindrical, ends rounded; nuclei large, single or double, Clevet, 56 17. COSMARIUM. FIG. 17. End view without central inflations (1). End view with central inflations (2). . Cytioderm smooth or punctate (a). si more or less verrucose or granular (/). ih spinous (g). ; Cytioderm smooth or punctate (Z). * more or less verrucose or granular (2). . Chlorophyll diffused (4). . Chlorophyll concentrated in 1 or more nuclei (e). . Margins crenate, undulate, dentate or granulate (c). f not crenate, undulate, dentate nor granulate (@). Margin crenate (s). ‘“« —undulate (f). “granulate (z). “dentate or notched; end truncate, smooth; sides toothed, CONVEX 8S got ac, tek AR CPMSCRER: OS Margin dentate; pak 4- sphidd: Sides seek convex, aculeatum, 66 . Cell twice or more longer than wide (7). . Cell 1% or less than twice longer than wide (A). . Cells less than 1% times longer than wide (7). *F.-w. Alge, p. 27. $8 SPECIES OF DESMIDS. *~ if 3 Se teks ~ aie | NON & ews. ®. SSS, Margins crenate, undulate, dentate or granulate (z). Margins smooth; basal angles not toothed (zw). Margins smooth; basal angles each with 1 tooth or papilla, Smolandicum, 69 Verruce or granules in patterns, or more or less restricted (2). am . . not in patterns; more or less covering the surface (cc). Spines on the whole surface; cell suborbicular; sinus elliptical, aculeatum, 66 Spines marginal, conical; centre with 7 granules, and punctate, trachypleurum, 73 . Margins crenate, undulate, or dentate (77). . Margins smooth (//). Verruce or granules in patterns, or more or less restricted (zm). -s . fe not in patterns, more or less covering the surface (vv). | End rounded; semi-cell oblong, sides almost parallel, De Baryz, 58 < “ semi-cell spherical, . . . . . moniliforme, 60 fr semi-cell elliptical or oval . cordanum,* oblongumt End truncate; semi-cell quadrate, sides almost parallel, anceps, 59 ef “«°, semi-cell pyramidal: 2300.0.) pa . Cell joined by pellucid bands into families . . . Quimbyit, 61 . Cells net joined into families (2). Ends rounded; semi-cells oblong ... . . . ¢cucumts, 58 i ‘“¢ * semi-cells oval; diameter ;44; in. (23,4), Witrockitt Ends truncate; semi-cells pyramidal, sides convex; diameter 4, to aise (22-284) . . . 2. motabile, 306 bath on truncate; semi-cells cyrotael sides convex; diameter x4, to shy (50-854) . . . . . . pyramidatum, 69 . Ends truncate; semi-cells pytamndal sides convex; diameter >;455 to zt5 heen sie ay ae . . . pseudopyramidatum, 69 Ends truncate; semi-cells asrasnidat: sides straight or slightly CONCAVE, 295% 2 bg ee yeramatmnr, Oe . Cell conspicuously phaddey chin ee semi-cells subsemicircular; side view circular, end view elliptical; diameter 32-38, scenedesmus, 59 m. Cell conspicuously droader than long; semi-cells subsemicircular; side view ovoid; diameter 75-854 .. . . . ctrceulare,** * Journ. R. Micr. Soc., Dec., 1885. + Journ. R. Micr. Soc., Feb., 1886. ** FW. Alge, p. 27 ef seg. Mm, 2. SPECIES OF DESMIDS. 89 Cell nearly as long as_ broad; semi-cells rhomboid, sides all equal: sinus deep, wide... <.. . . . rhombusoides.** . Ends rounded; semi- -cell subcircular, sinus a mere notch, globosum, 60 _ g semi-cell subsemicircular (0). . . semi-cell oval (7). 2 a semi-cell elliptical, or hexagonal-elliptical; cell 4 longer than wide; diam. 25-42, sexangulare, 63 . Ends rounded semi-cell elliptical; cell % longer than wide; diam. A POM 2 . . microsphinctum,** Ends truncate; semi- heel hemos diameter ag in. (21) or OS aaa . . . polygonum, 65 Ends truncate; semi- cell triangles: inmicses siz in. (50) or less. galerttum, 7° . semi-cell subsemicircular, smooth; sinus deep, nar- row . . . nitidulum, 62; psendonitidulum, 62 ve . punctate or granular; si- nus narrow ALC Ur Cede cum lobulatulum.* Sinus deep, narrow ( ). . Sinus deep, wide, almost linear; cytioderm smooth, sejunctum, 62 % rounded or oval; semi-cells lunately curved; cytioderm DUNC LAMe gs So a fematam, Os Sinus deep, wide, obtuse; semi-cells idenine from a narrow base, inflatum.* Sinus acute angled, enlarging outwardly, rarely linear, per foratum,* Per eluptical; basal anglés acute ©. 5°. 2... Batley, G4 . Cell suborbicular; basal angles obtuse; diameter 54, to z4, in. [DOXTOO} i ee eh a ae, OS : ca “ diameter giv to ghy in. £3-38), 85. Se wat eae 3s constrictum, 58 . Cytioderm centrally somewhat soaular nucleus 1 in each semi- Wee aah aay tumidum, 61 ¢ smooth or punctate; eelts shall bioculatum, 60; tinctum, 61 Peis rounded; semi-cells semi- porpiemiar; crene usually 9, undulatum, 67 x s¢ semi-cells subsemicircular; margins finely crenulate- MGHCAte re ee Ge eg a 2 oc ORR * Journ. R. Micr. Soc., Dec., 1885. + Journ. R. Micr. Soc., Feb., 1886. ** FW. Alge, p. 27 et seg. go SPECIES OF DESMIDS. s, Ends rounded; semi-cells subsemicircular, with 8-10 crenulations, Sendtnerianum,* s. Ends truncate; semi-cells pyramidal; cells small, . . motadbile, 66 Ss sides almost parallel; inmate gy tO ghy in. (30-384), . . . . erenatum, 67 S. sides converging; diameter ae: in. (20/4), Negelianum, 67 ¢. Semi-cell quadrate, smooth, angles rounded; end retuse or convex, guadratum, 59 Z. pyramidal or subquadrate; end undulate, Reina 68 £ as . end truncate; diameter =1, to <4, in. (32-36), integrum, 68 Z. ‘ rx a diameter 3p/5y tO qo'gy in. (20-24), | Hammert, 79 Z, ‘s sf end rounded, surface smooth, octogonum.* z. Semi-cell pyramidal, punctate, base flat, angles rounded, ansatum, 68; Vymannianum, 79 z. Semi-cell sub-semicircular, end truncate, sides with 2 .emargina- tions; smooth (0<))5'2. 7% Pie More P ee ie uz. Ends truncate. Diameter <4, to > tra in. (20-30f), punculatum, 74 uw. Ends convex. Diameter >s45 to ayy in (14-16) . . lave, 62 v. Cell twice as long as wide, rectangular; sinus linear, not widened, sinuosum, 05 v. Cell less than twice as long as wide; semi-cell pyramidal, venustum, 68 v. se " +4 ie semi-cell subquadrate, small, Meneghinit, 65 w, Sinus narrow, not eidied outwardly; cell elliptical, end convex, vartolatum 63; exiguum, 66 w. Sinus widened and rounded ete: narrowing outwardly, cell wider than long .. . : . . . obsoletum, 64 w. Sinus widened outwardly; semi- oan ‘oval hase and end convex, contractum, 63 WwW. a os " base and end flattend, depressum, 64 w. Sinus widened outwardly; semi-cell quadrate, Meneghinit, 65 w a Ss semi-cell subsemicircular, end rounded; diameter 51, to x4, in. (75-100) . . . . pachydermum, 70 x, Margins crenate or granulate (7). x. Margins not crenate nor granulate (40). * F.—w. Alge, p. 27 ef seg SPECIES OF DESMIDS. gti y. Central verruce none or scattered on each semi-cell (2). y- as more or less clustered on each semi-cell (aa). zg. Verruce none central, si ons I or 2 rows; cell about twice longerthan wide... Ce et ROE g. Verruce none central, Rcinal ¢ I or two rows; ea less than twice ionger than wile. triplicatum, 73; LVordstedti1, * gz, Verruce centrally scattered, marginal in series of 3 each; semi- cell quadrate, anglesrounded .... . . triplicatum, 73 aa. Central verruce 3, in a single row; surface smooth, Donnellii, 71 aa, ' 3, 2 above the base; punctate between, | polymorphum.* aa. “f 5, in 2 transverse rows, a row within the margin, guinarium,* aa. : 6, in a triangle, apex toward the isthmus, polymazum, 70 aa. se 6 or g, in 2 or 3 transverse rows; marginal rows I or 2; semi-cell semicircular, ($B eof) oy ca cee eg ea Mantes, Ox . Four times longer than wide, leach: diameter of (484); linear elliptical, nolip . . . . . pentoides,* . Four to six times longer than we: Gecath. or indistinttly punc- tate; front view tapering, lateral fusiform, ;,1,, toz,ky (20-22), levis, 91 19. XANTHIDIUM. FIG. 19. Spines divided atthe ends... . Seg es. LOIRE GS Spines subulate, ends not divided oe Spines more or less scattered, numerous . . . aculeatum, 92 $6 ag sf ra 4 within the margins, Columbianum'** . Spines marginal (4). . Basal angles with 2 spines (@). és ” i. I spine (c). . Other spines geminate in 4 pairs. . ee cristatum, 93 s - i in 2 pairs on the ene single on the sides, asteptum, 93 if not geminate, single on theends . . Torreyi.** . Other spines 6 to 10 pairs on semi-cell; protuberance beaded, bisenarium, 93 . Other spines 2 to 4 pairs (e). “s rN an ge gir rae OPE Ce RET Ole, (OS Other spines, 4 pairs, terminal . . . . . . . fasciculatum, 93 a “¢ 2 pairs, basal, vertjcal . . . . rectocornutum, 94 ee ‘« 2 pairs, terminal; a row of granules above the cen- tral projection, a spine above the granules, J/tnneapoliense, 94 . Other spines, 2 pairs, terminal; a row of granules above the cen- tral projection, no spine above the granules . polymazum, 94 . Other spines, 2 pairs, terminal, no granules above the projection : (/). . Diameter 74, in. (55-654) or more, Zylerianum+, fasciculatum, 93 as shy in. (50) or less; semi-cell truncate-triangular, — asteptum, 93 - ds . semi-cell not truncate-triangular, antilopeum, 94 *Journ. R. Micr., Soc., Feb., 1886. **F, W. Alge, p. 34 et seq. ¢Journ. R. Micr. Soc., Dec., 1885. 96 § § a. a. Semi-cell with more than 2 spines (¢). 6, Cytioderm with deciduous spines. . . SASS ocaey Cae ane 4. " verrucose in rows, margins cheouen . guadridens, 96 b. ig “ « ends alone crenate, xotochondrus.** ¢. Spines on the same side diverging (e). m # be parallel (g). ra * . converging See es OCR S Oe ad. Margin of semi-cell angular, each angle with 1 or two spines, octocornis, 97 e. End truncate; spines widely divergent . . .. . . Juncus, 97 e. End convex; spines moderately divergent (/). J+ Piameter of cell che (40M) Geo a aaa es : shyt (30-35) « . e..) CSubulatus, 96 i +" FE praia CAMOIE) digs iste 5 aetna: ere iets an an g. Nuclei, 2 in each oblong-oval semi-cell . . . . . fragilis, 95 g. Nuclei none; semi-cell oval, diameter z5/55 in. (20) . ovalis, 96 g: #5 semi-cell orbicular, diameter 574, (12), orbicularts 96 r¢ semi-cell elliptical; spines often very short, convergens Var., 95 g. 7 semi-cell triangular, sinus wide, isthmus cylindrical, triangularis, 36** 21, EUASTRUM, FIG, 21. § End lobe evidently distinct (a). § End lobe evidently not distinct (6). a. End lobe deeply notched (c). a. End lobe more or less concave or sometimes convex (g.) 6. End deeply notched (e). é. End more or less convex; semi-cell with 7 or 8 lateral, short, coni- CHUTOCER a ieee og A REE 104 End more or less convex; ace pe ioe ‘teeth ‘130 2 DERSME, AOe SPECIES OF DESMIDS. 20. ARTHRODESMUS, FIG. 20, a, 0. Cytioderm smooth (a). Cytioderm verrucose or spinous (4). Semi-cell with 2 spines (c). Margins smooth (d.) . Margins more or less spinous or beaded (/). Margins dentate (2). *Journ. R. Micr. Soc., Feb., 1886. **F, W. Alge, p. 34 ef seg. SPECIES OF DESMIDS, 97 d. Basal lobe deeply notched; basal /odule broadly marginate; central WARUIE ODTSUC 5 ew ee ab hlobatym, 98 d, Basal lobe undulate (7). a, «¢ rounded or angular (/), e. Margins smooth (A). é. cuspidate, spinulose or beaded (7). j. Basal lobe undulate (zw). Be " rounded or angular (2). g. Margins smooth (7) | ees cuspidate, spinulose or beaded (7). A. End lobes horizontal; diam. 45-504 . . . Wordstedtianum, 105 | bE it “5 diam. 35-409 . . . . . . Spinosum, 106 (Aiea P Uprignt, Civerging 3 4 formosum,; 103 z. Basal lobes deeply notched (4). rz . undulate (z). j. Cytioderm rough with conic granules; semi-cells with one large central inflation, a smaller one on each side, two on end lobe, verrucosum, 100 @, Basal lobes undulate (cc). R, $ rounded or angular (ee). 7. A short spine on the angles of end and basal lobes divaricatum, 104 /, A small projection on each side near the apex . compactum, 107 m, Cytioderm more or less tuberculate (7). m. 5 punctate (9). m. smooth (7). Beweuoercies basal, mostly 5 sl eo ow Creare, 101 nt. - R Conta A Marval | Gee So Bae, TOG 2. ie: scattered; end lobe with a tooth on each side, | ornithocephalum, * 0. Semi-cell 5-lobed, basal lobe emarginate, the lateral small, entire, pinnatum, 98 0. . not 5-lobed (/). p. Basal lobe with 1 lateral, subcentral tubercle, not emarginate, ampullaceum, 100 p. Basal lobe without lateral tubercle, slightly emarginate, affine, 100 p.: . s vs . not emarginate; diam. 22-25 p, adidelta, 99 p. ‘« “not emarginate; diam. 60-694 . . . wentricosum, ** *Journ. R. Micr. Soc., Feb., 1886. 98 SPECIES OF DESMIDS. vy. Semi-cell subrectangular, basal lobe very broad, end lobe partly in- cluded ‘between: the: lateral. . 50°. Moos. ehessam OF vr. Semi-cell more or less pyramidal (s). s. Diameter 4, in. or more (50-55). . . . . : Ewuerettense, 102 s. Diameter less then 54, in.; apical angles acute, * Porkornyanum, 104; erosum, 104 s. Diameter less than in.; apical angles rounded . elegans, 106 ¢, End lobe ona long slender neck; basal lobe with 6 protuberances, mammillosum, 102 ALE Suen vane Fe “« basal lobe without protuberances, insigne, 102 ¢, End lobe not on a long neck (z). uw. Basal lobe much wider than end lobe (z). tu. ‘ scarcely wider; diameter less than x4, in. (42) stmplex, 106 . Basal sinus narrow, basal lobes approximate (f) és wide, basal lobes widely separated . tutermedium, 102 Diameter 51, in, (1004) . . . . . . . . . magnificum, ** v v as less than in.; no pits on the surface 250 p ’ ansatum, 99; purum, ** Ww Ww ° « «4 pits on semi-cell . scrobiculatum, ** . End lobe beaded: angles of basal lobes beaded. ventricosum, 160 * dentate; angles of basal lobes dentate . simplex, 106 Ww. sy smooth, its angles spinous orcuspidate. rostratum, 106 x. Angles of end lobe and margins of basal each with 3 diverging spines, cuspidatum, 105 s “ with short a margins of basal dentate or Sranvlate os iki, delta a ik OIE Sears . Angles of end lobe gin one dauh orspine. . . . vrostratum 106 . Basal and central lobules both slightly emarginate, oblongum, 98 . Basal lobes slightly emarginate, central obtuse, multilobatum, 98 . End lobe columnar, margins nearly parallel, end truncate, attenuatum, 103 z. End lobe not columnar, partly included between the lateral lobes, oblongum, 98 8 n eS eS & z. aE ss not included (aa). aa, Cell 2-3 times longer than broad; diameter 54, in. (75,4), humerosum, 99 aa, Cell about twice longer than broad, diameter 45, in. (14), Lundellit, aa, ‘s 4s a - diameter 4, in. (75), pectinatum.** ** F -W. Algz, p. 36 et. seq. SPECIES OF DESMIDS. 99 aa, Cell % or less longer than broad (0d). 66. Semi-cells urn-shaped; diameter s4, in. (50) . urnaforme, too bb. - more or less quadrate; basal lobes horizontal, emargin- ate; protuberances minutely granulate. . . gemmatum, 101 66. Semi-cells more or less pyramidal, basal lobes emarginate, . insulare, 104 cc. Angles of end lobe acute cid as an A MANS.” LOG CC. és " rounded or Benue (id). dd. Diameter 1, to ~h, in (32-38). . «1... Omerme, 104 dd. i qo in. (144); length 51, (281) + + erassicollé, 105 dd. “ ass tO qolgy in. (20-22); length 51, (28), compactum, 107 ee. Angles of end lobe acute (/*). ee - F obtuse or rounded (gg). ff. End notch broad, gaping, the apices upright . . . dbinale, 107 fF os narrow, Close, the apices horizontal . . simplex, 106 gg. End broadly rounded, continuous with the sides; diameter jh, 2 GAL) oe ie: | 0btesamys 104 s i 5 : <6 semi-cells wedge- shaped; diam. 24-28 é . cuneatum,** gg. End elevated above the sides, a small Breeton near the apex on PROMI sy ee ee ale 8h COMPACLME, 107 . End elevated, no Pion peaiebtiod: Da aca eae BOR 22. MICRASTERIAS, FIG, 22. FIVE FORMS. Cell more or less circular (1). Cell oblong (2). End lobe narrow, lengthened into divergent arms (a). ‘ ‘3 not lengthened into arms, semi-cells 5-lobed (4). End lobe broad, not lengthened into arms (c). Semi-cell 5-lobed, lobes horizontal; end lobe with 4 arms (¢). £3 ef 2 vs end lobe with 2 arms (2). Hy s lobes not horizontal, approximate; no arms (aa). . Semi- cell 3-lobed, lobes horizontal; end lobe with 4 arms (¢). be ag - “s end lobe without arms (/). Semi-cell 5-lobed (4). . 3-lobed, lobes radiate (/). “Rad lobe not or slightly exserted (¢). " conspicuously exserted (7). SSR RNY NN NN FH HOD 100 SPECIES OF DESMIDS. c. Semi-cells 5-lobed (7). c. Semi-cells 3 or obscurely 5-lobed; lateral sinus shallow, obtuse; lateral angles mucronate . . . . . . . decemdentata, 113 d. Basal lobes with 3 linear processes on each side . muricata, 118 d. . without linear processes, but (e). e. Forked once only, margins finely serrate, Mahabuleshwarensis, 112 é. " ‘“‘ margins and surface spinous . . . spinosa.**™ A tf * “margins smooth . . . . Wordstedtiana, 113 e. Forked twice (lobules forked); cytioderm spinous . . sfinosa.* é. ‘6 cytioderm smooth; margins serrate, Hermanniana, 112 é. ss re ~ margins not serrate, Americana, 112 jf. End lobe nearly as wide as the basal, apices deeply notched (g). if a 3 s me s apices not deeply notched (Z). jf. End lobe much narrower than the basal, end convex, oscttans, 116 be “ is . - é *« end deeply emarginate, foliacea, 118 ve e ad See ei “« end slightly concave, Rabenhorstit.** g. Basal lobes furcate (with lobules), (7) &. zs not furcate (7). : Ah. End lobe convex, without prominences . . . . . J/aticeps, 115 h. ‘4 truncate, with 2small prominences . . . . recta, 112 h. retuse, basal lobe furcate . . . . . . Bailey, 118 z, " with 2 slender, transverse, bidentate projections, 3 guadrata, 117 z. End lobe without projections, convex; sinuses broadly rounded, Kitchellit, 116 e * without projections, concave; neck short; sinuses acut- isha PS oo ial ROT OnE. tee z. oy * és " neck long; basal lobes curved Upwards 6 0064 so ieee en ca a oe Jj. Basal lobeshorizontal, not curved . . . . . jpinnatifida, 116 Le e curved upward, narrow, expansa, 117; arcuata, 117; simplex.* | k, Basal and lateral lobes deeply furcate (with lobules), furcata, 111 R, “i