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Complimentary
FIRST ANNUAL REPORT
of
THE LAGUNA MARINE
LABORATORY
at
LAGUNA BEACH, ORANGE
COUNTY, CALIFORNIA
a
POMONA COLLEGE
MAY, 1912
Jy &
Published by
Tue Department or Brotocy, Pomona CoLLeGcE
tONT, CALIFORNIA
Craremont, C
SPONSOR
THE LAGUNA MARINE LABORATORY
PERSONNEL FOR 1911
"DR. A. J. COOK, Sponsor
“MISS C. K. RICE, Chaperon
PROFESSOR C. F. BAKER, Director and Editor of Report
CHARLES W. METZ, Manager
“CHARLES W. METZ *DAVID L. CRAWFORD
"BLANCHE E. STAFFORD ‘VINNIE R. STOUT
"MABEL GUERNSEY
“HARRY V. M. HALL “JOHN GUERNSEY
FRANK R. COLE LEON GARDNER
Now California State Commissioner of Horticulture at Sacramento.
Now Mrs. A. C. Dyer of Kinsley, Kansas.
Later fellow-assistant under Dr, David Starr Jordan at Stanford University; now ap-
pointee to fellowship in Columbia University under Dr. Bashford Dean.
4 Of this group, but with table for the summer of 1911 in Pomona College Biological
Laboratory. Later fellow-assistant under Dr. Vernon L. Kellogg at Stanford Uni-
versity, and assistant in State Horticultural Commission. Recently appointed an
assistant in Cornell under Dr. J. G. Needham.
Fellow-assistant, 1911-12, Department of Biology of Pomona College and candidate for
master’s degree. Later appointed fellow-assistant in University of California under
Dr. C, A. Kofoid.
6 Fellow-assistant, 1911-12, Department of Biology of Pomona College and candidate
for master’s degree. Later appointed fellow-assistant in Cornell University under
Dr. J. G. Needham.
Appointed fellow-assistant for 1912-13 in Department of Biology, Pomona College.
8 Appointed scholar-assistant for 1912-13 under Dr. Robt. H. Walcott in the University
of Nebraska.
9 Accepted candidate fellow-assistant for 1912-13 under Dr. W. A. Setchell in the
University of California.
ot =
cr
As a matter of contemporaneous history, and of general interest to many who
are deeply concerned in all that pertains to this work, it may be mentioned that during
the years 1910-12 the following Pomona graduates also pass on into advanced biological
work: Elizabeth Heald and Sarah R. Atsatt to fellowships in Zoology under Dr. Kofoid
in Berkeley; A. S. Crawford, to a fellowship in Cornell Medical; John A. Prizer, to post
of Entomological Expert to the San Diego Land and Town Company; R. S. Vaile, to
Horticultural Commissionership of Ventura county, California; A. R. Baird, to Horti-
cultural Commissionership of Inyo county, California; E. O. Essig, to Secretaryship of
California State Horticultural Commission; H. A. Weinland, to an assistant in the
State Horticultural Commission; John E. Graf, to an Assistantship-in-charge at the Gov-
ernment Entomological Station at Compton, California; C, F. Stahl, to assist Graf; H. H.
Warner, to student assitantship in plant breeding under Prof. Babcock at Berkeley; A. R.
Davis, to a research fellowship in the Shaw School of Botany of Washington University
at St. Louis.
edicatedto the men who, having seen
a great, neto opportunity to do »
something worth while inthe world,
wasted no time, but seised upon it
with avidity and staped with it—
fo the men with whom originated
the idea of a permanent #larine »
Laboratory at Laguna Beach, and
towhom is due the practical develop-
ment of the larger possibilities for
the project:
James T. Smith
LZ. N. Brooks
I. N. Isch
N. Philvrook
and to these other pioneers, who,
accepting the challenge of a great
public service, have been the first
in addition to those mentioned sr
above, to give of their means soo
towards the founding of this insti-
tution:
Che Laguna Beach Co.
§. A. Burroughs, Pres.
G. G. Heisler, Gen. Mgr.
Charles 39. Low
James Irvine
And others
4 first Annual Beport
OUR PROFESSION OF FAITH IN
THIS WORK
fr
A man’s education most perfectly adapts him for the greatest
success in any phase of human endeavor only when it has included
thorough training in scientific method—accurate observing, truthful
recording, and logical correlating.
Ti.
as an element in education (and
Biology—the study of life
so in the progress of the human race) represents one of man’s most
intimate and fundamental intellectual activities—it is the study of
his own life forces and of the myriad influences that determine his
own setting in nature.
In
The sea, that limitless fountain-head of immeasurable life, which
without cultivation has yielded so much to the nurture of the human
race, which with cultivation can yield as much food as our best agri-
cultural lands, and which, one day, when the land is crowded with
teeming millions, will go far toward feeding the then existing peoples
—the sea, I say, presents a multitude of pressing problems of infinite
importance to the future of humanity, and is the preeminent bio-
logical laboratory of earth, which every year is attracting an increas-
ingly greater number of students, and enlisting an ever warmer and
more active human interest.
C. F. BaKer.
Laguna Warine Laboratory 5
PROLOGUE
OR many years groups of Pomona College students in
Biology, had made annual visits with Dr. Cook, head of the
Department of Biology, to the neighboring coast, usually to
Deadman’s Island at San Pedro. The single day spent there
each year, among the rich displays of life accessible at low tide, had
kept alive the intention of the Department, growing stronger year
by year, to spend portions of each summer on the coast, with a
selected group of interested Pomona students. After many attempts”
to organize such a project, early in 1911, Dr. Cook, Professor Baker,
and Mr. Charles W. Metz, at a final conference, decided to go, regard-
less of obstacles, for one season’s trial, and to take with them a group
of students interested in the work solely for the work’s sake, and to
share with them alike in all expenses of whatever nature. The selec-
tion of a location was left to Professor Baker and Mr. Metz, and
these two, during the Spring of 1911, worked over, largely on foot,
most of the coast from Redondo to Laguna. The latter locality was
settled upon without any question, as by all odds the most desirable
for our immediate purposes—studies on the fauna and flora of the
tidal zone and its immediate neighborhood. The varied topograph-
ical and ecological conditions—the high promotories, the acres of
rocky tide-pools exposed at low tide, the numerous small sand
beaches, all equally accessible—together with the varied beauty of
all the natural surroundings, showed this to be a most uniquely
favorable place. We immediately rented a large house from Mr.
James T. Smith, and June 21 found us in possession with all the
necessary outfit for the proposed work.
Miss C. K. Rice (now Mrs. A. C. Dyer of Kinsley, Kansas) had
kindly consented to serve as chaperon, and the following students
joined the party: Blanche E. Stafford, Vinnie R. Stout, Harry V.
M. Hall, Mabel Guernsey, John Guernsey, Leon Gardner, and some-
what later, Frank R. Cole. David L. Crawford, who was also one
of this group, carried on his work in the Claremont laboratory. With
*In connection with these attempts, grateful acknowledgments should be made to
Miss M. Hathaway and Mr. Llewellyn Bixby.
6 Fitst Annual Report
the energetic assistance of Mr. Metz as business manager, and the
active co-operation of all, everything was in readiness for house-
keeping and laboratory work by the 26th of June. Three tents in
addition to the house were found necessary to accommodate all the
work proposed.
Kivery day thereafter to the end of the summer was full. Low
tide hours were used for collecting and work on the beach, and this
often took a party out at five a. m. or earlier. Every foray yielded
The 1911 Laguna Company
On porch at back, Miss Rice and Mr. Guernsey. In front, left to right, Pro-
fessor Baker, Mr. Hall, Mr. Cole, Mr. Metz, Mr. Gardner, Miss Baker, Miss Stafford,
Miss Stout, Miss Guernsey, and our host, Mr. James T. Smith.
loads of valuable material that required all the remaining hours for
study and preservation. Every day was rich in discovery and inci-
dent, and every nightfall marked substantial progress in the work of
everyone connected with the laboratory. An hour given every day
to a plunge in the surf kept the health of all in prime condition. All
the varied work of the laboratory and house was well organized and
progressed with a rare smoothness and lack of friction. Sunday was
given to rest, to writing letters, to reading, to strolls along the cliffs,
and to regular but informal ethical and religious discussions.
~I
Laguna Warine Laboratory
We consider that all accomplished in 1911 amounts merely to a
cursory preliminary reconnoissance—an effort to get aequainted
with a few of the most salient features of the local field. Some work
was done on the life of the fore-shore, more thorough work on the
distribution of life between tides, and a good deal was accomplished
in the tide-way with the tow-net. Mr. Metz was very active in the
collecting of tide-pool and shore fishes, extending his operations a
number of miles up and down the coast, working out the pools thor-
oughly, and also getting a good many things by line and net. He
also visited Newport, and through the marked kindness and interest
of an expert power-boat fisherman, Mr. J. HE. Souder, made several
trips off-shore, for the purpose of visiting the gill nets and jigging,
and also made arrangements for the saving of various rare things to
be found in the fishermen’s nets and traps. Mr. Souder also loaned
to the laboratory a skiff for use at Laguna, a favor for which he has
our heartiest acknowledgments. To him are due several of our most
valuable finds during the summer.
Of the many thousands of specimens gathered during the summer,
and preserved in best of order, not one small part has as yet been
worked up, though work is in progress along many lines, either by
students on the Coast, or by well known specialists elsewhere who are
co-operating with us. The results presented in this report are to us
simply a beginning—a breaking of the ground. We have to acknowl-
edge the kindly assistance in this work of many specialists through-
out the world, including Prof. J. M. Aldrich, of Idaho University,
Prof. Walter K. Fisher of Stanford University, Dr. M. Bernhauer of
Austria, Mr. J. H. Paine of Stanford University, Mr. 8. 8. Berry of
Stanford University, Dr. W. A. Setchell of California University,
Mr. Julius Hurter of the St. Louis Academy of Science, Dr. Wim. A.
Ritter of the San Diego Marine Laboratory, and others. Large col-
lections of marine worms and of sponges are still untouched, and will
be at the disposal of interested special students.
Acknowledgments should also be made to many friends at Laguna,
notably our host, Mr. Smith, whose kind and helpful interest was
with us always; to Mr. Isch, our banker and merchant; to Mr. Brooks,
who gave us many a boost; to Mr. Trefern, on whose patience we
drew heavily, and to others. Col. Coulter, whose untimely death has
lately been announced, was a frequent visitor to the laboratory, and
8 Sitst Annual Report
was one of Mr. Smith’s first converts to the idea that the laboratory
ought to be a permanent institution in Laguna. Our stay in Laguna
was marked by this kindliness on every hand. Laguna, unlike any
other place on the coast, possesses the rare charm of a quiet and
homelike country village—an unusual and valuable asset which in
itself is much sought and highly prized by great numbers of people.
Just this feature is doubly inviting, when the village happens to be at
A view of the building used for Laboratory in 1911, with surroundings. Professor
Baker’s tent at left, with Mr. Metz’s tent back of that. Mr. Guernsey and Mr. Hall
had tables on the upper porch, others had tables in the larger room on the first floor.
Mr. Gardner’s tent was placed back of the house to the right.
the most desirable point on the coast. We are hoping that nothing will
ever destroy that unique atmosphere.
During the summer several hundred visitors went through the
laboratory, expressing keenest interest in all they saw there, and to
them we took pleasure in extending every courtesy. Among these
visitors we were delighted to number many of the friends and alumni
of Pomona College. We were also visited occasionally by men from
government institutions, and from neighboring educational institu-
tions.
Laguna Warine Laboratory 9
Hspecially does the laboratory group feel deep gratitude for his
constant, helpful, and inspiring influence as sponsor of the whole
enterprise, to Dr. Albert J. Cook, now State Commissioner of Horti-
culture, then Professor of Biology in Pomona College, an earnest
friend of all who strive, an active laborer for all things good and true,
and an ardent believer in the possibility of the ultimate realization
of all the highest in human hopes.
Within the laboratory group, perfect kindness and good fellow-
ship was the great distinguishing feature.
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Figure 39
A, Laila cockerelli. B, Chromodoris porterae. C, Chromodoris sp. (mantel
removed). D, Chromodoris sp. E, Genus? F, Aegires albopunctatus. G, Ancula
pacifica. H, Cuthonia sp. I, Hervia sp. J, Hermissenda opalescens. K, Spurilla sp.
~]
~
Laguna Marine Laboratory
Laila cockerelli MacFarland
(Figure 39A)
Color translucent whitish. Branchiw and clavate papille tipped
with orange red. Rhinophores orange red with white bases. Numer-
ous small orange red tubercles on the dorsum. Orange red marking
also occur on the tail. The one specimen taken differed from Mac-
Farland’s description in having two instead of five branchial plumes,
but this is of little moment since the branchie are constantly subject
to injury.
Genus and Species?
(Figure 39 EH)
Color white, with two black stripes from mouth to rhinophores,
where they unite and pass as one to the branchiz. Sides irregularly
marked with black stripes and spots. Tentacles yellow. Rhinophores
black, tipped with yellow. Bordering the black stripes are various
spots and stripes of yellow. Many of the yellow spots occur on small
tubercles. The branchie are black, tipped with yellow.
Rhinophores foliate and retractile into small sheaths. Branchize
seven, and also foliate and retractile. Six short tentacle-like pro-
cesses on the head, which are joimed by a thin prolongation of the
mantle edge, this being practically all of the mantle that is evident.
Head large and truneated. Length about 7 mm.
Doriopsis fulva MacFarland
( Figure 38 B)
Color lemon yellow. Mantle thickly covered with small white-
tipped tubercles. Rhinophores brownish. Branchie a paler yellow
than dorsum. Front edge of foot deeply bilobed. No notch on upper
lip. Rhinophores and branchie retractile into small smooth edged
sheaths. Tentacles rudimentary, attached to lateral folds on the
sides of the mouth. Length 9 mm. Frequent under stones between
tides. Doubtless the determination of this would not be sure with-
out a comparison of the internal anatomy.
Rostanga pulchra MacFarland
(Figure 38 A)
Color orange red, the foot pinkish. Rhinophores darker than
mantle. Anterior margin of foot bilobed. Upper lip notched. Ten-
tacles small. Rhinophores very short and completely retractile.
Mantle covered with short spiculate papilla. Length 5 mm. Found
under stones, thus differing in habit from the species as described
by MacFarland for he records it from a red sponge.
78 First Annual Report
Doris sp.
(Figure 388 C)
Mantle brown, bordered with yellow, and covered with small white
tubercles more thickly in the center than on the edges. Foot yellow,
branchiew light yellow. The branchiw and rhinophores are retractile
into smooth bordered sheaths. Body deep, with the dorsum highly
arched. ‘Tentacles rudimentary. Length 27 mm. Common under
stones during July and August but rare in September.
Cuthonia sp.
(Figure 39 H)
Color translucent whitish. Cerata translucent with a dark green
core. A dark green spot in front of the rhinophores. Length 5 mm.
Cutting edge of mandible strongly denticulate. Radula consisting of a single row of
plates. There are usually nine teeth on a plate, the central one not prominent, as short or
shorter than the prominent laterals.
Hervia sp.?
(Kigure 39 I)
Color translucent whitish. Rhinophores orange, and with an
orange spot in front of them. Cerata with greenish-black cores, and
tipped with orange. Animal very slender, the body highest in the
region of the rhinophores. Rlhinophores and tentacles slender and
non-retractile. Length 7 mm.
Cutting edge of mandible not toothed, strongly striated. Radula consisting of a single
row of plates. Usually eleven teeth on a plate, the central not prominent, the laterals long
and slender.
Hermissenda opalescens (Cooper).
(Figure 39 J)
Color translucent whitish, with light brown stripes on tentacles
and tail. Orange markings occur on the head. Cerata translucent,
with brown cores and orange markings. Animal very slender. Cerata
numerous and very easily detached. Anterior margin of foot pro-
longed into two tentacle-like processes. Lips bilobed, the upper
narrow, the lower large. Common during the early part of the sum-
mer in tide-pools.
Cutting edge of mandible with about thirty strong denticles. Radula consisting of a
single series of angularly arched plates. The central tooth is large and finely denticulated
on the lower edge; the laterals are small, three to four on a side.
Spurilla sp.
(Figure 39 Kk)
Color white. Back and top of head orange with a finely granu-
lated appearance. Cerata near head orange with brown cores, those
farther back becoming browner. ‘Tentacles white. Rhinophores
foliate, and red, with white tips. Animal slender, the foot large,
anterior margin slightly prolonged into tentacle-like processes.
Rhinophores and tentacles slightly contractile. Mouth large, under
lip bilobed, upper smooth. Cerata in constant motion and very easily
detached. Common in September. Length 15 mm.
Cutting edge of mandible not toothed, strongly striated. Radula consisting of a single
series of low-arched, pectinate plates, notched in the center.
Laguna Warine Laboratory 79
Figure 40. Limax maximus
A, Left lobe of liver. B, Right lobe of liver. C, Reproductive organs. D, Ali-
mentary canal. E, Abnormal jaw. F, Normal jaw. G, Shell.
80
Sitst Annual Report
A, Liver.
mentary canal.
Figure 41. Limax flavus
B, Nervous system (ventral view).
E, Nervous system (dorsal view).
C, Reproductive system.
F, Jaw. G, Shell.
D, Ali-
Laguna Warine Laboratory 81
PULMONATA
Two species of Limax are common both at Laguna Beach and
Claremont. They have been provisionally determined by Mr. S. S.
Berry, as Limax maximus Linn. and Limax flavus Linn. They differ
in color, both being of a yellowish-gray, but Limav maximus is spot-
ted and streaked with black, while Limax flavus has no black spots.
There are also some anatomical differences. Limax maximus is,
when fully grown, about two inches long; Limax flavus is somewhat
smaller.
Figure 42. Circulation of Limax flavus
Limax maximus L.
(Figure 40)
Limax flavus L.
(Figures 41 and 42)
PELECYPODA
FILIBRANCHIATA
Mytilus californianus Conr.
(Figure 43)
The species is gregarious, forming extensive mussel-beds on flat
rocks exposed to the surf. There are several large colonies of them
at Laguna, notably the one at Mussel Point, which furnishes to the
villagers endless supplies for food and for bait.
Mytilus bifurcatus Conr.
More generally distributed along this coast than M. californianus.
Sometimes very numerous on rocks uncovered at low tide.
82 First Annual Report
Septifer bifurcatus Roe
Distribution same as Mytilus bifurcatus but rarer.
PSEUDOLAMELLIBRANCHIATA
Ostrea lurida Cpr.
Found in lower tide pools and on rocks uncovered but a short
time at low tide. Not at all common.
Figure 43. Mytilus californianus
EULAMELLIBRANCHIATA
Phacoides californicus Conr.
Semele rupium Sby.
Tivela stultorium Mawe
Chama pellucida Sby.
The above four shells are found washed up on the beach, the last
two quite common.
Laguna Marine Laboratory 83
ON A CEPHALOPOD NEW TO CALIFORNIA
WITH A NOTE ON ANOTHER
SPECIES
S. 5. BERRY
The two species of cephalopod mollusks (one squid and one
octopus) which are the subject of the following notes were obtained
by Mr. C. W. Metz during the 1911 session of the Pomona College
Marine Laboratory at Laguna, Orange County, California. Although
so few of this class of animals were taken, one of the two specimens
submitted to me represents a species new not only to California, but
apparently to the entire western coast of North America as well. I
am indebted to the gentleman named for the opportunity to examine
and report upon the material.
Figure 44. Funnel of Onychoteuthis banksii
Laid open along the medio-ventral line to show the funnel organ (semi-dia-
grammatic).
Onychoteuthis banksii (Leach 1817) Ferussac
1817 Loligo banksu Leach, Zool. Miscell., vol. 3, p. 141.
1826 Onychoteuthis banksu Ferussae, in D’Orbigny, Ann. Sci.
Nat. (1), vol. 7, p. 151.
1879 Onychoteuthis banks Tryon, Man. Conch. (1), vol. 1, p.
168, pl. 73, figs 291-294.
1908 Onychoteuthis banks Pfeffer, Nord. Plankton, IV, Ceph.,
p. 65, figs. 71-77.
A single female specimen of this widespread oceanic species was
obtained from J. H. Souder, who captured it in a seine off the en-
trance to Newport Bay, S. 8S. B. No. 295. In a report written some
84 First Annual Report
months ago and now in press, the writer expressed the opinion that
O. banksii would very likely be found to occur in the waters of our
region, but so prompt a confirmation of the statement was scarcely
to have been expected.
From all other West American species excepting only the gigantic
Moroteuthis of Alaska, O. banksii is readily distinguishable by the
double series of powerful hooks on the tentacle clubs as shown in the
accompanying photograph (Figure 45). The curious ‘‘fixing appar-
atus’’ at the base of the club (Figure 46) furnishes another con-
spicuous character. This structure comprises a compact, rounded
Figure 45
Showing hooked tentacle clubs of Onychoteuthis banksi1.
group of suckers and pad-like organs so arranged that the suckers
on one tentacle fit perfectly over the pads of its mate, securing a
most powerful adhesion at a point where such support very greatly
increases the prehensile power of the tentacles. The immense rhom-
boid fins are also characteristic.
So far as I have been able to determine from the literature the
present individual is by far the largest specimen of the species which
has happened to be placed on record. D’Orbigny* gives the total
length of O. banksii as 310 mm., mantle length 1380 mm.; of the
identical O. angulatat as 400 mm.; mantle 130 mm. Tryon gives the
*Moll, viv. et. foss., 1845, p. 387.
;Voy, Amer. Merid., Moll., p 43, 1845.
CO
oO
Laguna Marine Laboratory
Figure 46
Fixing apparatus of right and left tentacle clubs of Onychoteuthis banksii.
Figure 47. Polypus bimaculatus
86 first Annual Report
ordinary length of the body as six inches (i. e., about 150 mm.).
Joubin® records a specimen having a total length of 195 mm., mantle
110 mm. The body of our specimen is considerably over twice as
long as the largest here cited. Its more important dimensions are
therefore appended below:
Figure 48. Anatomical details of Polypus bimaculatus
A, Circulatory system (right kidney removed). B, Male reproductive organs.
C, Jaw. D, Female reproductive organs. E, Alimentary canal and liver. F, Ali-
mentary canal. From dissections by Miss Guernsey.
Length to tail 680 mm.; length, exclusive of arms, 353 mm.; length,
exclusive of tentacles, 488 mm.; length of mantle (dorsal) 313 mm.;
width of mantle 80 mm.; length of fins, total, 183 mm.; length of fins
along plane of attachment 164 mm.; width across fins, 215 mm.;
length of head 40 mm.; width of head 49 mm.; length of dorsal arm
101 mm.; length of second arm 120 mm.; length of third arm 116 mm.;
*Ceph. “Prine.-Alice,” 1900, p. 63.
Laguna Marine Laboratory 87
length of ventral arm 135 mm.; length of tentacle 327 mm.; length of
tentacle club 73 mm.
Polypus bimaculatus (Verrill 1883)
(Figures 47 and 48)
A specimen of this species (S. S. B. No. 324) was taken by Mr.
Metz in one of the lower tide-pools at Laguna, and numerous other
specimens by other members of the laboratory. It is the common
shore ‘‘octopus’’ of Southern California and has been previously
reported from White’s Point, San Pedro and San Diego. The large,
eye-like, lateral markings near the base of each arm of the third pair
constitute its most conspicuous specific character. In the present
specimen the usual bluish ring* surrounding the central spot is
obscured or absent.
A microscopic examination of a portion of the integument in the
neighborhood of these markings shows that the outer ring of the
oculation owes its pale color chiefly to a diminution in the number of
chromatophores over this area.t Similarly the dark center is due
to a great and sudden increase in their frequency. The exact number
is somewhat variable, but a given space in the dark center appears
to contain fully twice as many as an equal area in the paler border.
Even when expanded, all the chromatophores are excessively
small. In the present material the dimensions of one of these organs
is .09 x .16 mm. expanded, and .04.x .06 mm. in diameter contracted.
Their detailed structure is correspondingly difficult to make out.
The illustrations accompanying this paper were prepared by Mr.
John Howard Paine of Stanford University, Mr. Metz and Miss
Mabel Guernsey.
*Berry, Univ. Calif. Publ. Zool., vol. 18, p. 302, 1911.
~Whether the pigment within the chromatophores themselves is likewise differen-
tiated either in color or quantity, cannot of course be determined without examining fresh
material.
88 First Annual Beport
SOME ECHINODERMS COLLECTED
AT LAGUNA
Cc. F. BAKER
Starfish, serpent stars, sea-urchins, and sea cucumbers are very
much in evidence between tides at Laguna, some species occurring
in enormous numbers. As yet, no special effort has been made to
collect the species thoroughly. Of most of those taken a set was de-
termined by Prof. Walter K. Fisher of Stanford University, and he
very kindly furnished the notes incorporated below under quotation
marks.
HOLOTHUROIDEA
Synapta inhoerens O. F. Mull
These beautiful pale-colored little holothurians are frequent in
the sand under stones in tide-pools. Some of their movements are
extraordinarily worm-like. The peculiar character of the dermal
anchors and plates in this species are very distinctive.
Stichopus californicus (Stimpson)
The large brown sea cucumber is a common object in the tide-
pools. Some grow to eight and ten inches in length and even more.
We examined large numbers of them for commensals but did not
happen to encounter any.
ASTEROIDEA
Linckia columbiae Gray
The smooth red starfish is common in the tide-pools. Its capacity
for arm motion is very limited compared to other starfishes here, and
through frequent mutilations it is rarely normally armed. Prof.
Fisher says of it: ‘‘This curious little starfish is a member of the
Panamie fauna, the type having been taken on the west coast of
Colombia. It has been recorded previously from California at La
Jolla, San Clemente Island, Santa Catalina Island and San Pedro.
Miss 8. P. Monks carried on some interesting studies on the varia-
bility and autonomy of this species. It is able to sever its arms, and
not only to regenerate new arms, but also to regenerate new disks on
the severed rays. The number of rays varies from one to nine, but
there are usually five. There may also be more than one madreporic
body, and as many as four anal apertures. Very rarely there are
two mouths.’’
Laguna Marine Laboratory 89
Coscinasterias sertulifera (Xantus)
The ‘‘soft starfish’? is very common in the tide-pools. It is one
of the most unpleasant starfishes to handle, due to its extraordinary
sliminess. Its soft body gives a very wide range of possibilities in
movement. Of this species Prof. Fisher remarks: ‘‘This is the
species (under the name Asterias ferreri) upon which Prof. H. S.
Jennings carried on a number of experiments at La Jolla. It is a
member of the southern fauna, the type locality being Cape San
Lucas. The true Coscinasterias ferreri belongs to the northern fauna
and is not found along shore.’’
Pisaster capitatus (Stimpson)
Not common in the tidal pools, but evidently much more numerous
just at and below low tide mark. Prof. Fisher says of it: ‘‘This
species grows to a large size and is characterized by the heavy, well
spaced tubercles of the back. It was formerly included in the genus
Asterias.’’
OPHIUROIDEA
Ophioderma panamensis Lutken
Apparently the largest serpent star at Laguna, and abundant in
the lower tide-pools. Its smoothish body and commonly bright and
varied shades of brown and yellow make it a very conspicuous species.
Prof. Fisher remarks of it: ‘‘This is a common littoral serpent star
from Panama to Catalina. It has a finely granulated disk and four
genital openings on each interbrachial space.’’
Ophioplocus esmarki Lyman
As this species occurred to us at Laguna, it was smaller than the
foregoing, and usually unicolored, instead of variegated. Prof.
Fisher records this as occurring from San Diego to Monterey.
Ophionereis annulata LeConte
With a heavy vestiture and comparatively small, this serpent
star occurs in great numbers under stones in tide-pools, sometimes
dozens under a single stone. Prof. Fisher says: ‘‘This is a common
form from Central America to Southern California. It has long,
cross banded arms, short arm spines, and a disk covered with fine
overlapping scales.’’
Ophiothrix spiculata LeConte
We found this species only in kelp holdfasts from three to six
fathoms, and common in large sponge masses. It is common in these
places and is doubtless common also under other conditions. Prof.
90 Sitst Annual Report
Fisher says of it: ‘‘This is one of the most beautiful of echinoderms,
being characterized by many long thorny spines which are delicate
and glassy. It ranges from Monterey Bay to Central America.’’
ECHINOIDEA
Strongylocentrotus franciscanus Agassiz
This is the large, commonly blackish purple, long spined sea
urchin, usually found singly in the lower tide-pools. As Prof. Fisher
remarks, it is commonly bright purple or even reddish purple. It
is far less common than the following.
Strongylocentrotus purpuratus (Stimpson)
The bluish-purple gregarious sea urchin occurs in large colonies
in some of the lower tide-pools. One such pool at Mussel Point con-
tains a remarkable display of these urchins. Many of them are seated
in deep, cylindrical bores in the rock from which it is frequently
impossible to extricate them. They apparently do not favor pools
that are beyond the reach of low tide spray.
Dendraster excentricus (Eschscholtz)
“The common sand dollar of the curio store. It was formerly
included in the genus Echinarachnius’’ (Fisher). The shells of this
species are occasional on the beach at Laguna.
Laguna Warine Laboratorp 91
STUDIES IN PYCNOGONIDA, I
HARRY V. M. HALL
In all our collecting but twenty-two pyenogonids were taken,
twenty of which belong to the same species and one of the others to a
different species of the same genus. The commonest had a spread of
legs of about three-fourths of an inch, while the other species was
about three times as large. The latter were so nearly the color of the
fueus on which they lived and so covered with debris that it is pos-
sible that they may be much more numerous than the number taken
would indicate. In working out this report I am indebted to Dr.
Leon J. Cole for many kind suggestions and much valuable assist-
ance. I have described the species found as follows:
Anoplodactylus californicus n. sp.
(Figure 49)
Body rather short, lateral processes about as long as their own
diameter, radiate, with bases contiguous. First two intersegmental
lines barely visible. Proboscis cylindrical with rounded end, almost
as long as the length of the body. Diameter of the proboscis one-
half its length. Eyes not apparent, but a large conical eye tubercle
(bent to the right in the cut as is also the abdomen) arises from the
anterior edge of the body which projects over nearly the first half
of the proboscis. The abdomen is much the same shape as the last
joint of a man’s middle finger and, like the eye tubercle is deeply and
closely pitted. The rest of the body is pitted but less deeply. Cheli-
fores large with well developed chele and stout shaft, the whole
reaching about half their length in front of the proboscis. (In my
specimen the chele are extended straight in front but there seems to
be no reason why they might not be bent in front of the proboscis.)
A few short spines on the chele; basal joints grown together and
apparently supporting the eye tubercle. Palpi and ovigerous legs
very rudimentary and wholly within the body (see plate for details).
The legs are rather long but stout, sparsely set with short spines.
First coxa shorter than its diameter, second coxa over twice the
leneth of the first and enlarged at its distal end, third coxa one and
one-half times the length of the first. Femur longer than the com-
bined length of the coxe. Tibial joints each about three-fourths the
leneth of the femur. All joints of the lees stout. Tarsus about one-
half the length of tibial joints; claw two-thirds the length of tarsus
Sitst Annual Report
Figure 49. Anoplodactylus californicus
Laguna @atrine Laboratory 93
and folding down to rows of fine hairs. Auxiliary claws very small.
Genital openings not apparent. Color straw. Measurements in mm.
Proboscis 1.424; body (from anterior edge to insertion of abdomen)
1.5; leg (approximately) 8; diameter of lateral (leg-bearing) pro-
cesses .428,
This specimen was swept from fucus at low tide and was put in
a bottle with a small nudibranch mollusk which we caught about the
same time. About half an hour later we discovered this pyenogonid
greedily feeding on the nudibranch. This is of special interest as
very little is known of the feeding habits of these interesting crea-
tures.
This species bears a superficial resemblance to Pallenopsis, how-
ever it differs from that genus in the following respects, i. e.: The
abdomen is neither long nor slender, there are no eyes apparent,
and the ovigerous legs, instead of being ten-jointed and present in
both sexes, are in my specimen reduced to the merest rudiments and
are within the body wall so that externally they do not show. On
the other hand it is not a typical Anoplodactylus, the body being
more compact than is usual in that genus, though not nearly so
compact as that of 4. anarthrus (Loman).
Ammothella bi-unguiculata var. californica n. var.
(Figure 50)
Body distinctly segmented, leg-bearing processes moderately
separated and moderately developed. Their length is about one-half
their diameter. Intersegmental lines all distinct. Proboscis slim,
spindle shaped; in length two and one-half times the diameter, and
four-thirds the length of the body; ending in front with a rounded
obtuse angle as seen from above. Four eyes in pairs on a very low
eye tubercle; well pigmented. Abdomen small, cylindrical, less than
one-fourth the length of the body, with bluntly rounded tip. Anus
in notch at the tip. Chelifores short, one-sixth the length of the
proboscis, three-jointed; chele undeveloped; first joint very short,
shaft not quite as long as terminal joint which is nearly.spherical.
Diameter of chelifores slightly less than that of the palpi. Palpi
nine-jointed; as long as the proboscis. First joint short and thicker
than the others. Second joint four times as long as the third; fourth
joint almost as long as the second; fifth and sixth joints about the
same length as the third. Terminal joints decrease in order. Very
few hairs on the palpus except on the terminal joint. Ovigerous legs
slightly longer and with slightly greater diameter than the palpi.
The ten joints named in the order of their lengths (except the first
which is short and much thicker than the rest) are, 4, 2, 5, 6, 3, 7,
8,9, 10. The terminal joints are spirally rolled and on the tip of the
Laguna Warine Laboratorp 95
last are three stiffly plumose hairs. cE 0
vy, mf phe =
os
ar) : ,
|
i |
Figure 67. Ligyda occidentalis (Dana)
A, Dorsal view. B, Mandible. C, Second maxilla. D, First maxilla. E, Max-
illiped. F, First antenna. G, First leg of male.
Laguna Warine Laboratorp 123
Figure 68. Ligyda occidentalis (Dana)
A, Fourth leg of male. B, Fifth leg of male. C, Second leg of male. D, Third
leg of male. E, Second antenna. F, Second pleopod of male. G, Third pleopod
of male. H, First pleopod of male. I, Uropod.
124 first Annual Report
sharp and short. Uropoda about half the length of the body, 11
mm.; peduncle 3.5 mm., and about two-thirds as long as the outer
‘amus; outer ramus slightly shorter than inner, which is armed with
a single spine.
Alloniscus perconvexus Dana
(Figure 69)
Locality—Abundant in sand of upper beach under kelp; very
abundant at high tides when driven out by the water.
Color—Dull gray mottled with black and white.
3ody ovate and convex, about twice as long as wide, 15 mm. by
7 mm. Head has frontal margin produced in the middle into a
rounded lobe; antero-lateral angles into an acute process extending
slightly beyond the eyes. Eyes composite and situated close to
lateral margins. First pair of antenne very small and rudimentary,
consisting of three small articles. Second antenne composed of five
articles and a flagellum. First two articles short, third and fourth
longer and subequal, fifth over one and a half times as long as fourth.
Flagellum has three subequal articles. Second antenne thickly cov-
ered with small spines. Mavxillipeds have a palp of three articles.
Palp of mandible wanting.
First segment of thorax longer than the others, which are sub-
equal. On the first segment of the thorax a faint suture line extends
a short distance from the posterior margin and at an obtuse angle,
indicating the epimeron. On the next three segments sutures extend
the length of the segments. On the last three segments epimera are
not visible. Legs ambulatory, thickly spined.
Abdomen consists of six segments, five subequal in length, the
sixth somewhat longer. First two are covered laterally by the last
thoracic segment. Last abdominal segment triangular and narrower
than all the preceding segments. Uropoda very short, about the
leneth of last abdominal segment; inner branch about half as long
as outer.
The whole surface of the body is covered with minute spines.
Idothea rectilinea Lockington
(Figure 70)
Locality—Abundant in pools, under rocks, on Phyllospadix and
among fucus at low tide.
Color—Male usually a light brown; female usually a very dark
brown with antenne and legs of ight brown.
Body narrow and elongate; sides almost parellel and segments
very closely articulated; five times longer than wide, 20 mm. by 4
mm. Dorsum depressed longitudinally on either side of median line.
Laguna atrine Laboratory 125
Figure 69. Alloniscus perconvexus (Dana)
A, Dorsal view. B, Maxilliped. C, First maxilla. D, Mandible. E, Seventh
leg. F, First leg. G, Second maxilla. H, Third pleopod of male. I, Second
pleopod of male. J, Mandible. K, First antenna. L, First pleopod of female. M,
Second antenna. N, First pleopoda of male.
126 first Annual Report
Figure 70. Idothea rectilinea (Lockington)
A, Dorsal view. B, Maxilliped. C, Mandible. D, Second maxilla. E, Second
antenna. F, First maxilla. G, First antenna. H, First leg. I, Second leg. J,
Seventh leg. K, Posterior pleopod of male. L, First pleopod of male. M, Second
pleopod of male.
Laguna Barine Laboratorp 127
Head wider than long; anterior margin broadly emarginate and
slightly narrower than posterior margin. Eyes very small, com-
pound and placed on the middle of the extreme lateral margin. First
antenne have four articles; basal article enlarged much _ broader
than the other articles. Fourth article clavate and finely haired on.
the superior margin. Second antenne composed of peduncle of five
articles and a flagellum of twelve. Basal article of peduncle very
small. Second slightly longer than wide; third somewhat longer
than second and almost twice as long as wide; fourth and fifth about
equal in length and longer than the third. The maxilliped has a palp
of four articles. The mandible is without a palp.
The first segment of the thorax is only half as long medially as
laterally. The rest of the segments are more regular in shape and
sub-equal in length; last two slightly shorter than the others; pos-
terior margins of all slightly excavate. The epimera of second and
third segments oceupy shghtly less than half the lateral margin;
epimeron of fourth occupies about half and like the other two is very
narrow; epimeron of fifth segment is broader and occupies about
two-thirds the lateral margin; epimera of sixth and seventh seg-
ments occupy the whole length of lateral margin.
The legs are all similar in structure; propodus di-dactylous and
slightly haired with short hairs; the three preceding segments pro-
vided with longer hairs which are more abundant except on the last
two pairs of legs, where they are very scant.
Abdomen consists of three distinct and one partially coalesced
segment. First segments are very short, last is long and produced
medially to a point.
Philoscia richardsonae Holmes and Gay.
(Figure 71)
Locality—Under old seaweed on the edge of a salt marsh, La-
guna Beach, California.
Color—Brown, microscopic examination shows distinet pigment
markings over the whole surface.
Body oblong ovate; length slightly over twice the greatest width,
6 mm. by 2.5 mm. Surface of body thickly covered with fine spines.
Head twice as wide as long, with arched frontal margin and
lateral angles subacute. Antenna long; first article of peduncle
about half as long as second; second and third subequal in length;
fourth twice as long as third and narrower; fifth somewhat longer
than fourth; flagellum composed of three articles, of which the sec-
ond is slightly shorter than the first or third; the latter ends in a
large spine and the whole antenna is provided with numerous short
spines, especially on the margins. Antennule rudimentary, only vis-
128 first Annual Report
Figure 71. Philoscia richardsonae (Holmes and Gay)
A, Dorsal view. B, Second maxilla. C, First maxilla. D, Mandible. E, Second
antenna. F, Maxilliped. G, First pleopod of female. H, Second pleopod of female.
I, Terminal segment of abdomen. J, First leg. K, Seventh leg. L, Third pleopod
of female. M, Antennule.
Laguna Barine Laboratory 129
ible by dissection, composed of three small articles. Maxillipeds
have a palp of three articles. Palp of mandibles wanting.
Segments of thorax subequal; post-lateral angles of last three
very marked and produced. Epimera not distinct. Legs all similar,
ambulatory, very much spined and increasing in length from first
to seventh.
Abdomen abruptly narrower than thorax; first five segments sub-
equal. Post-lateral angles of third, fourth and fifth produced. Last
abdominal segment produced medially. Median length about half
the greatest width. Basal segment of uropod about as wide as long
and extends beyond last abdominal segment; inner ramus a little
less than half as long as the outer; both rami are spined.
Dynamene glabra Richardson
(Figure 72)
Locality—F ound on Phyllospadix at Laguna Beach, California.
Color—Pale orange brown, somewhat mottled, with white spot
on telson; become dark brown in alcohol.
30dy convex ovate, about twice as long as wide, 3 mm. by 114
mm.; surface smooth.
Head small and rounded anteriorly. Eyes placed in the post-
lateral angles. First pair of antenna with first article of peduncle
less than twice as long as wide; second and third articles subequal
and narrower that first. Flagellum composed of twelve articles.
Second antenna with first article of peduncle about as long as wide,
small; second twice as long as first; third about the same length as
first; fourth similar to second; fifth one and a third times as long as
fourth. Flagellum composed of sixteen articles. Mavxilliped pro-
vided with a palp of five articles. Mandible with a palp of three
articles.
Thorax has first segment longer than those succeeding, which
are subequal. Post-lateral angles of last three are produced. Legs
all ambulatory; dactylus bi-ungulate.
Abdomen has the penultimate segment composed of several coal-
esced segments, though the suture lines were not clear in the speci-
mens at hand at the time of the drawing. Other specimens found
later showed two short suture lines. Last abdominal segment trian-
gular with a median fissure at the posterior extremity. Inner ramus
of uropod about as long as telson and immovable; outer branch half
as long as inner, and movable.
Cirolana harfordi var. spongicola n. var.
(Figure 73)
Locality—Sponges at low tide mark, Laguna Beach, California.
Color—White, but marked with fine black dots.
130 first Annual Report
Figure 72. Dynamene glabra (Richardson)
A, First leg. B, Second leg. C, Third leg. D, Fifth leg. E, First pleopod of
male. F, Sixth leg. G, Seventh leg. H, Maxilliped. I, Second maxilla. J, Man-
dible. K, Uropod. L, Telson.
Laguna Watrine Laboratorp 7 13
Body very convex and oblong, about three times as long as wide,
7 mm. by 2.5 mm. being the dimensions of a small specimen.
Head twice as wide as long with frontal margin rounded. Eyes
composite, occupying lateral margins of the head. Second antenna
has a peduncle of five articles; the first three are small; fourth about
as long as first three; fifth longer and narrower than fourth; flagel-
lum composed of twenty-two articles; in five specimens examined the
number of articles varied from twenty-two to twenty-nine, average
twenty-four. Antennule has a peduncle of three articles; first two
small and subequal; third half as wide and equal in length to the
first two; flagellum composed of twelve articles, average out of five
specimens examined, ten articles; maxillipeds composed of seven
articles; last five provided with many long plumose spines; third
article has two blunt hooks. Mandible robust, broad and strong
distally where it ends in a tridentate margin. Mandibular palp has
three articles. Frontal lamina short and broad and is sub-trian-
gulate at the distal end.
First segment of thorax longer .than the following three, which
are subequal. Last three longer than the three preceding. Hpimera
distinct on all but first segment. Last four segments marked with
an oblique, first two with a longitudinal carina. Epimera of last
three, especially of last two, segments produced at post-lateral
angles. First three legs prehensile, last four ambulatory. In the
first pair the propodus has three spines on the inner margin which
increase in size toward the distal end, where one long slender spine
also occurs; on the outer distal end there are one or two slender
spines. The carpus usually has but one long spine; the merus has
five or six thick, blunt spines on the inner margin and two or three
sharp ones. On the outer distal end there are two long slender
spines; the ischium has one blunt spine on the inner distal margin
similar to the blunt spines on the merus; there is one long, stout one
and one long, slender spine on the outer distal end. The propodus
of the second and third legs has on the inner margin four spines
increasing in length toward the distal end. The carpus has three
blunt spines on the inner distal end. The merus has eight or nine
large spines on the inner margin; one long and strong and from one
to four shorter ones on the outer distal end. The ischium has two
very blunt spines at the inner distal end, and two or three smaller
spines at the outer distal extremity. Last four legs provided with
many stout spines.
Abdomen composed of six visible segments. Last, triangular,
gradually narrows to a rounded apex which is provided with about
eight teeth and numerous long hairs. Peduncle of uropod is pro-
duced to two-thirds the length of the telson; inner ramus is wider
132 first Annual Report
Figure 73. Cirolana harfordi var. spongicola
A, Dorsal view. B, First maxilla. C, Mandible. D, Maxilliped. E, Frontal
lamina, clypeus and labrum. F, Second maxilla. G, Second antenna. H, First an-
tenna. I, First leg. J, Second leg. K, Fourth leg. L, Seventh leg. M, First pleopod
of male. N, Second pleopod of male. O, Telson and uropod.
Note:—The ‘extremities of both the inner and of the outer rami are very
distinctly serrate, especially the outer.
Laguna Marine Laboratorp 133
than the outer and somewhat longer, provided with spines and teeth.
Outer branch is also thickly spined. Both the inner and outer rami
are very markedly serrate, especially the outer, in which the extrem-
ity is so sharply divided that it appears bifurcated.
These specimens found in the sponges appear to be very closely
related to Cirolana harfordi (Lockington) but the marked difference
in the number of spines at the extremity of the telson and the evident
difference in the extremities of the uropod rami, which are more
rounded and much less emarginate in Cirolana harfordi seems to
warrant a varietal distinction. The lateral margins of the telson
of Cirolana harfordi are curved near the base; in the specimens
obtained from the sponges these margins are straight. These differ-
ences, with those of size and habitat, allow at least a distinction in
variety.
134 Fitst Annual Report
STUDIES IN LAGUNA AMPHIPODA
VINNIE R. STOUT
Orchestia traskiana Stimpson
(Figures 74 and 75)
Numerous specimens taken agree very well with the description
of O. traskiana in Das Tierreich, except in having the distance be-
tween the eyes varying from considerably less to a little more than
one diameter, also in having the flagellum of antenna II with the
flagellum somewhat longer than peduncle and 16-18 jointed in male,
all the female specimens observed having fourteen joints. Color,
dull shades of grayish brown and green, with the appendages bluish.
Very common under decaying alge about edges of a brackish
water slough which is reached by salt water only at times of extreme
high tides. Laguna Beach, California. Coll. V. R. Stout.
Amphithoe corallina n. sp.
(Figures 76 and 77)
The color of the body varying from a fairly bright brownish
green 282 (Valette) to dull orange-green 182 and 178; antenne about
tone of green 203; other appendages light and transparent; back,
sides, legs, and antenne spotted with white; females carrying bright
orange colored eggs.
Body robust and smooth. Head very broad and deep. Hyes
small, subrotund, black. Antenne one and two nearly the same in
length, a little more than half as long as the body. First joint of
peduncle of antenna one a little longer than the second joint and
almost three times as long as the third; flagellum about twenty-six-
jointed and a little longer than the peduncle. Antenna two stouter,
peduncle longer than the twenty-four-jointed flagellum; ultimate
joint of peduncle a little shorter than the penultimate. Lower lip
similar to that of A. flindersi. Mandibular palp large, third joint
longer than second and armed with six long spines. Maxilla two
normal with inner plate fringed with compound hairs. Gnathopods
one and two about the same length; gnathopod two broader and more
thickly setose. Second joint of persopods one to five slightly expand-
ed, joint of pereopod three most strongly so. Propodus of
pereopods one and two strongly narrowed distally; propodus of
pereopod four expanded distally, and propodus of persopod five
slightly expanded distally. Pleopods one to three thickly clothed
with long sete; basal joint of rami about one-third as long as pedun-
Laguna Marine Laboratory 135
Figure 74. Orchestia traskiana
A, Adult male. B, First maxilla. C, Second maxilla. D, Mandible. E, Lower
lip. F, Upper lip. G, Maxillipeds. H, First antenna. I, Second antenna. J, First
gnathopod of female. K, First gnathopod of male. L, Second gnathopod of female.
M, Telson.
136 first Annual Report
cle; rami subequal. Uropods one to three normal, two reaching not
quite as far back as one and three; none of the rami as long as their
peduncles; outer rami in one and two shorter than inner, in three
longer than inner. Telson somewhat broader than long, bluntly tri-
angular, with several lateral spinules. Length 6-8 mm.
Laguna Beach, California. Occasional in tufts of coralline alge
between tides. Coll. V. R. Stout.
Amphilochus litoralis n. sp.
(Figure 78)
Entire animal colored a delicate pink, varying from red 21 to 3B
(Vallette).
Body smooth, stout, and quite compact, the dorsum strongly
arched. Rostrum long. Side-plate very small and convex in front.
Antenne short and stout, less than one-fourth the length of the body.
Antenna one with the six-jointed flagellum longer than the peduncle.
Antenna two more setose, and with the peduncle nearly twice as long
as the six-jointed flagellum. Upper lip incised, with somewhat un-
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Figure 75. Orchestia traskiana
A, Second gnathopod of male. B, First peraeopod. C, Third peraeopod.
D, Fifth peraeopod. E, Third uropod. F, First uropod. G, Second uropod. H,
First pleopod.
Laguna Marine Laboratorp 137
Figure 76. Amphithoe corallina
A, Adult male. B, Second antenna. C, First antenna. D, First maxilla. E,
Second maxilla. F, Lower lip. G, Maxillipeds. H, Mandible. I, First, second and
third pairs of uropods, and telson.
138 first Annual Report
Figure 77. Amphithoe corallina
A, First gnathopod. B, Second gnathopod. C, First peraeopod. D, Second
peraeopod. E, Third peraeopod. F, Fifth peraeopod. G, Fourth peraeopod. H,
Third pleopod. I, First pleopod.
Laguna Warine Laboratory 139
Figure 78. Amphilochus litoralis
A, Adult male. B, Right mandible. C, Second maxilla. D, First maxilla.
E, Left mandible. F, Maxillipeds. G, Inner plate of maxillipeds. H, First antenna.
I, Second antenna. J, Second pleopod. K, First gnathopod. L, Palm and finger of
first gnathopod. M, Second gnathopod. N, Palm and finger of second gnathopod.
O, Fifth peraeopod. P, First peraeopod. Q, First, second and third pairs of uropoda,
and telson.
140 Sitst Annual Report
symmetrical front lobes. Lower lip without inner lobes; outer lobes
edged with long, slender spinules. Mandibles with three-jointed
palp; third joint of palp longer than both the first and second, and
armed with short, scarcely discernible setules. Left mandible with
inner plate, which is more finely toothed than the outer; molar obso-
lete. First maxilla with small inner plate bearing a single spine at
the slightly concave apex; outer plate with seven spines at the oblique
apex, and a bunch of small sete at the inner edge of the spine-row;
two-jointed palp with three short spines at the apex. Outer plate of
maxilla two-tipped with three compound spines, and fringed on the
outer margin with slender sete; inner plate tipped with six com-
pound spines. Maxillipeds with slender inner plates reaching about
to distal end of the first joint of the palp, and bearing three short
spines at the apex; outer plate broader, extending a little beyond
the first joint of the palp, the broadly rounded distal margin bearing
one long spine, several small spines on the inner part, and finely
serrate on the outer part; the joints of the palp gradually diminish-
ing in size from the first to the finger.
Gnathopod one the smaller, fifth joint with spines extending
almost to the palm, which bears ten slender spines. Second gnath-
opod with the sixth joint widening to the oblique palm, the front
margin of which bears a row of twelve small spines, and at the apex
two strong spines; in neither one does the finger extend quite to
the apex of the palm. Permopods one and two slender, nearly equal.
Pereopods three to five with second joint greatly expanded into a
broad, flat plate, gradually increasing in size to the fifth. The rami
of the subequal pleopods bear long, finely compound sete. Uropod
one extending almost to distal end of uropod three, peduncle much
longer than the subequal rami. Uropod two much shorter, the
peduncle of equal length with the inner ramus; the outer ramus
narrow and more than half as long as the inner. Uropod three
extending beyond uropods one and two; peduncle much longer than
the rami; inner ramus the longer. Telson about half as long as the
peduncle of uropod three, about twice as long as broad, and con-
verging slightly to the pointed apex. Length 2 mm.
This species resembles most closely A. marionis, but differs
especially in the characters of the mandible, maxillipeds, uropods,
and telson.
Laguna Beach, California. Common in Phyllospadixa. Coll. V.
R. Stout.
Genus Dulichiella n. gen.
Body slender; perwon segment one the shortest, sixth and sev-
enth not coalesced. Pleon of six segments beside the telson, third
most elongate; some of the pleon segments toothed posteriorly.
Laguna Watrine Laboratorp 141
Head not produced in front. Antenne strong and setose; antenna
one much longer than antenna two, flagellum longer than peduncle,
accessory flagellum present; antenna two with peduncle about twice
as long as flagellum. Mouth parts normal. Gnathopod one sub-
chelate, fifth joint longer but not broader than sixth. Gnathopod
two in female and left gnathopod two in male similar to gnathopod
one, though with the sixth joint longest and strongest. Right gnath-
opod two in male with sixth joint very large and powerful. Pere-
opods one and two long and slender. Persopods three to five stronger
and very long; much alike, fifth slightly longest; second joint ex-
panded, sixth joint longer than any other except second. Uropods
one and two with rather strong, unequal rami. Uropod three a con-
cave, spinose plate projecting over lateral margin of telson. Telson
rectangular.
This genus is evidently nearest the genus Dulichia, but differs
from it particularly in the character of the first antenna, second
right gnathopod of male, shape of perzeopods three to five, presenec
of rudimentary third uropods and shape of telson.
Figure 79. Dulichiella spinosa. Adult male
Dulichiella spinosa n. sp.
(Figures 179 and 180)
Body colored a pale, delicate green 228C (Valette), speckled
with dark red 27.
Body slender and smooth. Head longer than broad, but not
greatly produced in front. Eyes large, black, and irregular. An-
tenna one with thirty-eight-jointed flagellum, which is longer than
the peduncle; accessory flagellum five-jointed; second joint of
142 Fitst Annual Report
Figure 80. Dulichiella spinosa
A, Second antenna. B, First antenna. C, Lower lip. D, First maxilla. E, Max-
illipeds. F, Mandible. G, Second maxilla. H, Second right gnathopod of male.
I, First gnathopod of male. J, Second left gnathopod of male. K, First paraeopod
L, Fifth peraeopod. M, First pleopod. N, First uropod. O, Second and third pair
of uropoda, and _ telson.
Laguna Warine Laboratorp 143
peduncle somewhat longer than the first joint and about three times
as long as the third joint. Antenna two about two-thirds as long
as antenna one; thirteen-jointed flagellum about one-half as long
as peduncle. Upper lip slightly bi-lobed. Mandibular palp quite
large, second joint broader and longer than the third, both armed
with long, slender spines. Maxilla one with rather large, triangular
shaped inner plate, fringed with fine sete on the inner edge, and
armed with one compound, apical spine. Maxillipeds with outer
plates much larger than the inner, and carrying several compound
sete along the apical margin, finely toothed and setose along the
inner margin; palp long and strong. Pleopods rather large and
very much alike, the sub-equal rami bearing long, compound sete.
Telson long. Pleon segments all toothed posteriorly. Length 5-7
mm.
Laguna Beach, California. Frequent in kelp holdfasts from deep
water. Coll. V. R. Stout.
Genus Acanthogrubia n. gen.
D>
Head without rostrum. Side-plates one to five well developed,
fifth the broadest and deepest. Antenna one not as long as peduncle,
with four-jointed accessory flagellum. Antenna two, two-thirds as
lone as antenna one, with peduncle slightly longer than flagellum.
Mouth parts prominent. Upper lip broadly rounded. Lower lip
with well developed inner lobes, and bifid outer lobes with prominent
mandibular processes. Mandible normal, third joint of palp broadly
rounded at apex, and armed with numerous long sete. Mavxilla one
armed with ten apical spines, second joint of palp broad with several
spines on the apex and inner margin. Mavxilla two outer plate the
broader; inner plate as long as outer and thickly fringed on inner
margin. Maxillipeds, outer plates large. Gnathopods one and two
subechelate, gnathopod two the larger. Perswopods one and two long,
slender, second joint slightly expanded. Pereopod three, second
joint expanded, broader than long. Perawopods four and five the
longest. Uropod three, outer ramus with two hooks. Telson short
and broad, armed only with sete.
This genus differs from Amphithoe in having an accessory flagel-
lum on the first antenna. It also differs from Paragrubia in having
a four-jointed instead of one-jointed accessory flagellum, in having
the flagellum of the second antenna not elongate, and the second
joint of the third pereopod as broad as long.
144 first Annual Report
Nay’
me
Figure 81. Acanthogrubia uncinata
A, Adult male, the lines separating the two lobes of side-plate 5 to be considered
as a groove. B, Upper lip. C, Lower lip. D, First maxilla. E, Mandible. F, Second
gnathopod. G, Maxillipeds. H, Third pair of uropoda, and telson.
Laguna Warine Laboratorp 145
= Se
NS
Figure 82. Acanthogrubia uncinata
A, First uropod. B, Second maxilla. C, First gnathopod. D, First antenna. E,
Second antenna. F, First peraeopod. G, Second uropod.
146 First Annual Report
Acanthogrubia uncinata n. sp.
(Figures 81, 82 and 83)
Entire body varying between the two tones of orange-green 152
and 177 (Valette). Flagella of antenne ringed with white. Eyes
bright rose.
Body robust. Head produced in front of eyes. Side-plate one
produced forward. Side-plate five with very deep front lobe and
small hind lobe. Eyes small, subrotund. Antenna one, first joint of
peduncle slightly longer than second, and four times as long as third;
flagellum forty to sixty-jointed, accessory flagellum as long as first
four joints of primary flagellum. Antenna two, stout, setose; sec-
ond joint of peduncle longer than third, and about twice as long as
first; flagellum thirty-one-jointed. Mandible, palp stout, third joint
fringed with compound sete on the broadly rounded apex, principal
and secondary plates dentate, toothed spines in spine-row, molar
small. Maxilla one, outer plate with ten spines, second joint of palp
broad with nine small spines around the apex and inner margin,
with a diagonal row of slender setules around the whole joint.
Maxilla two, both plates thickly fringed with compound sete. Mavxil-
lipeds, outer plates fringed with two rows of short spines; inner
plate thickly fringed with long, compound sete.
Gnathopod one, fifth joint long, but not so long as the oval
sixth; sixth joint setose, palm not defined, finger faintly serrate.
Gnathopod two in female thickly setose, similar to gnathopod one,
but with shorter and broader fifth joint; enlarged sixth joint, with
palm distinct and eurved, finger longer than palm and _ faintly
serrate. Permopods one and two, second joint long and narrowly
rectangular, sixth joint narrowing distally. Persopod three, second
joint expanded, as broad as long, sixth joint with fine spines. Perso-
pods four and five much longer and proportionately narrower; sec-
ond joint somewhat expanded and narrowing towards the distal end;
sixth joint with six spines and not narrowing distally. Pleopods
with unequal rami fringed with long compound spines. Uropods one
and two, peduncle longer than the unequal rami. Uropod three,
peduncle a little longer than the rami, with a transverse row of
short spines on the apical margin; outer ramus with two spine-like
hooks; inner ramus broader with a transverse row of short spines,
one apical spine, and a row of seta. Telson as broad as long. Length
8-23 mm.
Laguna Beach, California. Occasional in tubes in kelp holdfasts
from deep water. Coll. V. R. Stout.
Laguna Marine Laboratory 147
Figure 83. Acanthogrubia uncinata
A, Fifth peraeopod. B, First pleopod. C, Third peraeopod.
148 Sitst Annual Report
Figure 84. MHyalella azteca
A, Adult female. B, Maxillipeds. C, First maxilla. D, Mandible. E, Second
maxilla. F, Lower lip. G, Second gnathopod of male. H, Second gnathopod of
female. I, First gnathopod. J, Second antenna. K, First antenna. L, Second
peraeopod. M, Third peraeopod. N, Fifth peraeopod. O, First uropod. P, First
pleopod. Q, Second uropod. R, Third uropod. S, Telson.
Laguna Warine Laboratory 149
Hyalella azteca (Sauss)
(Figure 84)
Body smooth, slightly compressed. Pleon segments one and two
each produced into a small dorsal tooth. Side-plates one to four
somewhat rectangular. Eyes nearly round. Antenna one, five to
eight-jointed, flagellum longer than peduncle; joints of peduncle suc-
cessively diminishing in length. Antenna two, slightly longer, the
seven to ten-jointed flagellum longer than peduncle; joints of
peduncle successively increasing in length. Maxillipeds, inner plate
tipped with three short, stout spines, and edged on the inner side
with slender compound sete; outer plate with nine toothed spines
and very small palp. Mavyilla two, both plates apically fringed with
fine spinules, which, on the inner plate, are edged with two plumose
sete. Mandible normal, principal and secondary plates dentate,
plumose spinules in spine-row, molar large, with compound seta.
Gnathopod one, small, slender, fourth joint with small pellucid
boss on the distal hind end; fifth joint with bulging hind margin,
fringed with row of spines and short teeth; sixth joint not so wide,
but sub-equal in length, hind margin covered with fine, short hairs,
curved finger reaching to end of the transverse, toothed palm.
Gnathopod two in both male and female, with bulging fifth joint
edged with spines, sixth joint in male very large and stout, longer
than broad, oblique palm with two notches underneath the strong,
curved finger. Finger not reaching to hinder margin of palm, but
closing between two spines just in front of a third notch. Gnath-
opod two in female, longer and stronger than gnathopod one, fifth
joint considerably produced behind, sixth joint twice as long as
widest part; hind part produced somewhat beyond the small curved
finger and the convex, transverse palm. Perzopods three to five,
second joint broadly expanded, serrate; pereopods four and five
nearly equal in length. Pleopods plumose. Uropod three, ramus
slender, tipped with sete, and subequal to peduncle. Telson, broad
as long; rounded apical margin with slender seta on either side of
tip.
Specimens agreeing well with description of H. azteca in Das
Tierreich, except in being smaller, and in having, commonly, fewer
joints in the antenne. They differ from the family description of
Talitridae in having the inner plate of maxilla one tipped with three
plumose sete, and in having the fine spinules of the inner plate of
maxilla two edged with two plumose sete.
Color varying from light bluish green to light green and brown.
Frequent in fresh water pond about five miles inland from
Laguna Beach, California. Coll. H. V. M. Hall.
150 first Annual Report
SOME DIPTERA OF LAGUNA BEACH
F. R. COLE
Laguna Beach is an excellent collecting ground for Diptera. The
hills and inland vegetation extend almost to the ocean in many places,
and with the varied topography and conditions, the insects are very
varied. The beach with its rocks, sand stretches, beach plants, and
decaying wrack, is swarming with Diptera. A small, shallow brack-
ish water lagoon at the inner edge of the beach gave some interesting
results in Diptera, but Diptera were collected on the rocks even to
within reach of the salt spray.
The collection of this year is but a mere beginning. I spent only
a few weeks at Laguna, and a large part of this time was consumed
in anatomical studies. Most of the flies reported in the present
paper are either peculiar to the beach itself, or are found in the
varied vegetation of the upper beach.
Without extensive assistance from Prof. J. M. Aldrich of the
University of Idaho, it would have been impossible to present this
paper at this time. Messrs. Knab and Hine have also kindly made
determinations. The accompanying habit sketches of new species
will be amplified by drawings of anatomical details, during the com-
ing summer.
Q
Figure 85. Scatcpse californiana n. sp.
CULICIDAE
Aedes squamiger Coq.
_ This mosquito was common and occasionally annoying at Laguna,
breeding in great numbers in the small brackish water lagoon. De-
termination by Mr. Knab.
Laguna MWatrine Laboratorp 151
BIBIONIDAE
Scatopse californica n. sp.
(Figure 85)
This is a small, slender black fly with polished body. The abdo-
men is about eight times as long as wide, and the head is long, narrow
and flattened. The antenne are large for the size of the head, and
the eyes are long and narrow. The abdomen is thinly pilose, the
hairs on the last segment and hypopygium longer than the rest. The
tibia and tarsi are slender and broadened at the apical ends. ‘The
body is spineless. Last joint of antenna longest. Proboscis entirely
black. Wings clear hyaline. Length 3 mm.
A single specimen collected by Prof. Baker. Apparently the first
Scatopse noted as peculiar to the Pacific Coast.
Figure 86. Euparyphus lagunae
STRATIOMYIDAE
Euparyphus lagunae n. sp.
(Figure 86)
A black, yellow marked fly, with a rather broad and flattened
body. The last three joints of tarsi are black, the first two black on
the distal ends. Body thinly pilose with long yellowish hair. Head
flattened. Femora and tibiew orange yellow, with no bristles. Front
black and very narrow. Base of antenne with two yellow stripes,
152 first Annual Report
which pass part way down the face. Four basal joints of the anten-
ne with long dark bristle-like hairs. Last joint of antenne slender
and twice as long as any other joint. Two longitudinal narrow yel-
low stripes on the thorax do not quite reach the scutellum. Halteres
lemon yellow. This fly is much darker than EF. bellus or E. ornatus.
Thorax above the wing with a small yellow patch, and a round yellow
patch on the lower corner of each abdominal segment as seen from
above. Last segment with base only black, the remainder yellow.
Ventrally the first segment of the abdomen is black, the rest sordid
yellow. Thorax black ventrally; there is a lateral narrow yellow
stripe running from the base of the wing, the length of the thorax.
Occiput convex and black. Mouth parts yellow. Most of the sub-
costal, and the end of the costal cell, is brown, the rest of the wing
hyaline. Length 5.5 mm.; length of wing 5 mm.
A single specimen collected by Prof. Baker at Laguna Beach.
BOMBYLIDAE
Toxophora pellucida Coq.
Several specimens.
THEREVIDAE
Psilocephala costalis Lw.
Psilocephala laevigata Lw.
Psilocephala marcida Coq.
The above three flies were taken in vegetation just back from the
beach.
ASILIDAE
Mallophora fautrix O. S.
Not uncommon. This species was described from Mexico.
Stichopogon trifasciatus Say.
(Figure 87)
This Asilid is widely distributed in the United States. At Laguna
it was common on the beach sand. It is a silver gray fly, with black
markings, and its colors blend with the sand perfectly. This pro-
tective coloration makes it very hard to locate when at rest. In
habits it is a typical robber-fly. Determined by Prof. Hine.
EMPIDIDAE
Drapetis nitida Melander
This fly was very common on the beach sand. It is quite small
and jet black, and runs rapidly here and there over the sand, seldom
flying to any distance. It is also found on the edge of the lagoon
and is very quick and hard to catch.
Laguna Watrine Laboratory 153
Figure 87. Stichopogon trifasciatus
vA
Figure 88. Parathalassius melanderi
154 First Annual Report
Parathalassius melanderi n. sp.
(Figure 88)
A small black fly, silvery gray pruinose, with all the bristles white.
The hypopygium is darker. The vertex is broad and concave, and
the face below the antenne narrow, broadening again at the gray cly-
peus. The antenne are dark brown, the first two joints small, the
terminal arista slender and about twice as long as the third joint
of the antenne. The lower half of eye covered with fine white
pubescence. ! Ga
sae Ope
Figure 122.
Figure 123. Herposiphonia sp.
Laguna Marine Laboratory
Figure 124. Heterosiphonia subsecunda (Suhr.) Falk.
211
DAD Sitst Annual WBWeport
BONNEMAISONIACEAE
Ricardia montagnei Durbes and Solier
Commonly epiphytic on Laurencia virgata.
RHODOMELACEAE
Laurencia virgata J. Ag.
(Figure 116)
This is a very variable species in both form and habits, growing
either on rocks or on the basal portions of Phyllospadix. In the lat-
ter case the stems usually exhibit a remarkable tendril action. Diam-
eter of the stems is commonly 1.5 mm.
Figure 125. Dasya pacifica Harv.
Laurencia pinnatifida (Gmel.) Lamx.
On rocks of the lower tidal zone.
Polysiphonia verticillata Harv.
(Figure 117)
Epiphytie on other alge of the lower tidal zone.
Pterosiphonia woodi (Harv.) Falk.
(Figures 118 and 119)
Commonly epiphytic on Nereocystis. Length of cells commonly
2mm. Diameter of cystocarps, .4 mm.; tetraspores .12 in diameter.
Laguna Marine Laboratorp
Figure 127. Griffithsia sp.
214 : First Annual Report
Pterosiphonia bipinnata (P. and R.) Falk.
Epiphytie on Nereocystis.
Pterosiphonia baileyi (Harv.) Falk.
Occasional on rocks of low tidal zone.
Strebdocladia camptoclada (Mont.) Falk
(Figures 120 and 121)
Abundant as an epiphyte on other alge of the whole tidal zone.
>
Cystocarps .8 mm. in diameter.
= y a
ie
G = Sa
Figure 128. Callithamnion rupicolum f. pygmaeum Collins.
Strebdocladia sp.
(Figure 122
Abundant on rocks and shells of the lower tidal zone. Of this
plant, Dr. Setchell says: ‘‘Possibly a Strebdocladia; perhaps, how-
ever, a Polysiphonia. I have had it before, but as yet have not sat-
isfactorily placed it.’’
Laguna Warine Laboratorp Di
Herposiphonia sp.
(Figure 123)
This was found in tow-stuff from the tide-pools, so that it is prob-
ably an epiphyte on other alge growing in such situations.
Lophosiphonia obscura (Ag.) Falk.
Common on low rocks of higher tidal zone, growing in dense col-
onies and acting as a sand gatherer.
Figure 129. Callithamnion rupicolum f. pygmaeum Collins.
Lophosiphonia villum (J. Ag.) S. and G.
Occurs intermingled with L. obscura.
Heterosiphonia secunda (Suhr) Falk.
Frequent as an epiphyte on various alge, especially corallines.
Dr. Setchell remarks that ‘‘this is the plant which has passed in our
lists under the name of Dasya subsecunda. The name given in the
list is merely a synonym. There seems to be some reason for sus-
pecting that our plant is not identical with the type of the species
given, but belongs rather to H. pulchra of the Japanese coast.’
216 Fitst Annual Report
Heterosiphonia subsecunda (Suhr) Falk.
(Figure 124)
Hpiphytic on other alge. Stichidia .8 mm. in length.
Dasya pacifica Harv.
(Figure 125)
Occasional in beach wrack. Stichidia .45 mm. in length.
CERAMIACEAE
Spermothamnion snyderae Farlow
(Figure 126)
Occasional in small colonies on the under side of ledges in the
lower tidal zone. Sporangia .1 mm. in diameter.
Griffithsia sp.
(Figure 127)
A common epiphyte on the alge of the lower tidal zone. Spor-
angia .08 mm. in diameter. Dr. Setehell says of this plant: ‘‘The
specimens all have tetraspores. I wish very much that it might be
found with cystocarps. Until then we shall be uncertain as to the
genus. Griffithsia is perhaps hardly to be applied to this plant. In
all probability it is more likely to belong to one of the related genera,
but it is impossible to decide this with certainty until we have the
eystoearpic fruit.’’
Callithamnion rupicolum forma pygmaeum Collins
(Figures 128 and 129)
An oceasional epiphyte on other alge of the lower tidal zone.
Sporangia .07 mm. in diameter. Cystocarps .2 mm. in diameter.
Antithamnion floccosum (Muell.) Klein
An epiphyte on Nereocystis.
Spyridia filamentosa (Wulf.) Harv.
Rare in the beach wrack.
Ceramium codicola J. Ag.
(Figure 130)
Common on Codium mucronatum, length commonly 1 em.
Centroceras clavulatum (Ag.) Mont.
Abundant on the rocks of the upper tidal zone.
Microcladia coulteri Harv.
Epiphytie on Prionitis.
Laguna Warine Laboratory 217
GRATELOUPIACEAE
Prionitis decipiens (Mont.) J. Ag.
Occasional on rocks between tides.
Grateloupia prolongata J. Ag.
Common on the rocks of the entire between tide zone.
Figure 130. Ceramnium codicola J. Ag.
Polyopes bushiae Farlow
One specimen found in wrack on beach.
NEMASTOMACEAE
Schizymenia sp.
One specimen collected in wrack on beach.
218 first Annual Report
CORALLINACEAE
Choreonema thuretii (Born.) Schmitz
Common in the middle tidal zone.
Lithothamnion sp.
Apparently several species of this genus form a common coating
on rocks throughout the tidal zone. Dr. Setehell remarks of them,
‘‘No one knows the species of the crustaceous corallines.’’
Amphiroa tuberculosa forma typica S. and G.
Amphiroa tuberculosa {. frondescens (Kuetz) S. and G.
The above two forms are common on the rocks of the lower tidal
zone.
Corallina officinalis L. var.
A common coralline among rocks of the middle tidal zone.
Corallina gracilis Lamx.
Frequent on rocks toward the lower tide mark.
Jania crassa Lamx.
Occasional on rocks about lower tide mark.
Jania rubens (L.) Lamx.
Occurs in middle tidal zone.
Lithothrix aspergillum J. E. Gray
Frequent on rocks of the middle tidal zone.
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