Presented to the UNIVERSITY OF TORONTO LIBRARY by the ONTARIO LEGISLATIVE LIBRARY 1980 Penna., Harrisburg, 2 S 5) ~ o —— = =| = 2 '@) <3) nN ns oa 2) = on) ri Ms a aA me NN 1 EON = St ty 23 os} aS —_—-_— T) beste = 5) ie _ of delegates and guests at the Group tu * a "6 >\\ SF pete 3? eRe: = O12 ‘Ss THE CONFERENCE.” Called by the Governor of Pennsylvania to ease ssc Ways and Means for Preventing the Spread c of the Chestnut Tree Bark Disease (t heg Frac SHE, CAPITOL Chamber of the House of Representatives HARRISBURG - PENNSYLVANIA FEBRUARY 20 and 21, 1912 Stenographic Report of Proceedings of the Conference Reported by GUILBERT & LEWIS 519 Land Title Building Philadelphia, Pa. ; HARRISBURG: 0. E. AUGHINBAUGH, PRINTER TO THE STATE UF PENNSYLVANIA 1912. 7 ! Bow ft Pi th ERR URSA MICROFILMED BY UNIVERSITY OF TORONTO LIBRARY MASTER NEGATIVE NO.: e.. 13aiag Sees FOREWORD. The following report of the proceedings of the Harrisburg Chestnut Blight Conference is distributed with the compliments and best wishes of the State of Pennsylvania. The numerous papers and the discussions thereon contain many new and valuable ideas. It is believed that the ultimate worth of the Conference will lie in the fact that it brought home to the east- ern United States the truth concerning a most serious tree dis- ease, and started discussions and a new trend of thought which must evolve real benefit for the whole people. If the Conference can produce a better understanding and higher appreciation respecting the value of trees, and of one tree in particular, its calling will have been of great public benefit. The officers chosen by the meeting take this last opportunity of expressing to the Governor, the Chestnut Tree Blight Com- mission and the Delegates from the States, their sense of high appreciation for the honor conferred upon them in being invited to preside over the deliberations of the Conference. They also have hopes that information may be found in the following pages which will incite greater interest in the earnest work now being undertaken in Pennsylvania and other States to prevent the fur- ther spread of this serious and destructive Chestnut Bark Dis- ease. LCL ¢ New York, Chairman, Pennsylvania, t Secretaries. Maryland, (3) ¢ G OFFICIAL CALL FOR CONFERENCE. The Official Invitation for the Chestnut Tree Bark Disease Conference, issued by the Governor of Pennsylvania. Se Cae of linnsyloanta COW “lly wn hes YO Me he furcsent a he bapitol Zp Harvitoy on Mhe Land YS Wf) flay. D IU2 Me, furtr Vide W0 q Convento walled, YZ, he purifies of ONMM IY lhe dango probed ty Mw prwatine and ircadr if a pongo décae f thowwld Ctl: free hncunw Eos Chest Lark Disease and the anithods l he pursed Za arm fpliting ti fe allecontiol ‘ ) . >: = 2 ? A CvccuttiieD ofp arbvnent, e Vasrve iharg, GF on dh SMMNUWOY LIA? Mir carly aid yf potitle AL “i walle vofilyy tothe Levlay of Vhe’ Denndyloania then Pree hight GomainissionDr Harold feirce.xoom W/2 Boogvis Buildin GY. Vlad pha, wld le i pectaled aa a ‘ = . : 4-2 ae es Oe ee ee a Seg, | i re A ir Py ee ey ee , 7 a ma | sla ae hy ia ee 7 "i ca 7 < re ee POTTER TIOGA CEN-TEFR =a ¢ Ri 2 L Cc AWrTORE Map of Pennsylvania Showing Infected Zones and Percentage. 1. Bucks, Montgomery, Chester, Delaware and Philadelphia counties, SO per cent. 2. Pike, Monroe, Carbon, Northampton, Lehigh, Berks, Lancaster and York counties, 50 per cent. 8. Wayne, Lackawanna, Wyoming, Luzerne, Co- lumbia, Montour, Northumberland, Union, Snyder, Juniata, Perry, Dauphin, Schuylkill, Lebanon, Cumberland, Franklin and Adams counties, 15 per cent. 4. From the western boundary of these counties to the quarantine line indicated on the map, the infected trees are estimated at 1 to 5 per cent. COMMONWEALTH OF PENNSYLVANIA THE PENNSYLVANIA CHESTNUT TREE BLIGHT COMMISSION. The call issued by the Governor, in which he urged the im- portance and necessity for prompt and concerted action in com- bating the Chestnut Bark Disease, included the following statement: “In 1911, the Pennsylvania State Legislature passed a bill au- thorizing the Governor to appoint a Commission of five citizens for the purpose of thoroughly investigating the Chestnut Tree Bark Disease which is rapidly destroying the chestnut trees of the Commonwealth. The Act placed an appropriation of $275,- 000 at the disposal of the Commission for the investigation and scientific study of the problem, and more specifically to ascer- tain the exact extent of the blight, and to devise ways and meaus through which it might, if possible, be stamped out, The Commission was appointed in June, 1911, and, after or- ganization, began its work immediately by sending a large force of experts into the field. The reports of these experts together with the results of the work of the pathological staff, will, among other matters, be presented for discussion to a Convention called by the Governor to assemble at Harrisburg, February 20th, 1912. In order that the other States not yet touched by the blight, but certainly in its line of advance, may realize the seriousness of the situation, the Governor, who is much interested, has called this Convention for a consideration of ways and means, in the hope that the States may be aroused to action and be ready to meet the invasion at their borders. Pennsylvania’s problem is now or soon will become the problem of Maine, Vermont, New Hampshire, Massachusetts, Rhode Island, Connecticut, New York, New Jersey, Delaware, Maryland, Virginia, North Caro- lina, South Carolina, Georgia, Alabama, Mississippi, Tennessee, Kentucky, West Virginia, Ohio, Indiana and Michigan. Active co-operation of the States is essential. The attendance of a large number of Delegates is respectfully urged.” (7) /) WW) COMMONWEALTH OF PENNSYLVANIA PROGRAMME of THESGONFERENCE Called by the Governor of Pennsylvania to Consider Ways and Means for PREVENTING THE SPREAD of the CHESTNUT TREE BARK DISEASE February 20 and 21, 1912 THE CAPITOL Chamber of the House of Representatives HARRISBURG - PENNSYLVANIA An office for registration and information will be opened in one of the ante-rooms of the House of Representatives, and it is earnestly requested that all delegates and guests will promptly register. (9) (10) PROGRAMME OPENING SESSION Tuesday, February 20, 2 o’clock P. M. ORGANIZATION OF THE CONFERENCE. 1. Call to Order and Address of Welcome to Delegates and Visit- ing Friends, by the Honorable John Kk. Tener, Governor of Pennsylvania. Election of Permanent Chairman for the Conference. Hlection of Two Secretaries. Designation of Official Reporters. Appointment of a Committee on Resolutions. 2. Responses to the Governor’s Address by Delegates on Behalf of the States Represented. 3. “Historical Review and the Pathological Aspects of the Chest- nut Bark Disease.” A discourse and illustrated lecture by Dr. Haven Metcalf, U.S. Department of Agriculture, Washington, D.C. (Dr. Metealf’s paper will summarize the record of work to date, and present the leading pathological features of this tree disease. ) Many of the lantern views will be shown for the first time, having been especially made for this occasion. 4. “Can the Chestnut Bark Disease be Controlled?” By Prof. F. C. Stewart, N. Y. Agricultural Experiment Station, Geneva, N. Y. | (ual) 12 5. “How Further Research May Increase the Efficiency of the Control of the Chestnut Bark Disease.” By Prof. W. Howard Rankin, Cornell University, Ithaca, Neve 6. “Recent Notes on the Chestnut Bark Disease.” By Prof. H. R. Fulton, Division of Pathology, Pennsylva- nia State College. =~ . “The Possibility of a Medicinal Remedy for Chestnut Blight.” sy Dr. Caroline Rumbold, in charge of the Pennsylvania Chestnut Tree Blight Commission’s Laboratory. 8. “Treatment of Individual Trees,” By Prof. J. Franklin Collins, U. S. Department of Agri- culture, Washington, D. C. 9. General Discussion. EVENING SESSION Tuesday, February 20, 8 o’clock, P. M. 1. “Chestnut Culture.” An illustrated lecture by Prof. Nelson I’. Davis, of Buck- nell University, Lewisburg, Penna. In this lecture Prof. Davis will exhibit the value of the chestnut trees as a source of food (nuts), and outline the progress made in the new American industry, chestnut cultivation. The insect enemies of the chestnut, and the methods of con- trolling them will be shown. Many of the views have been especially prepared for the oceasion, and will be shown for the first time. 2. General Discussion. MORNING SESSION Wednesday, February 2l, 9 o’clock, A. M. HKADICATION AND CONTROL OF THE CHESTNUT BARK DISEASE. 1. “The Pennsylvania Programme.” By Samuel bB. Detwiler, Executive Officer of the Pennsyl- vania Chestnut Tree Blight Commission. to . Reports by State Foresters, or other officials of States repre- sented, on the present extent of the bark disease and esti- mate of the present and possible future loss. 3. “Chestnut Blight and the Future of our Forests.” By Dr. H. P. Baker, Department of Forestry, State College, Penna. 4. “Chestnut Blight and Constructive Conservation.” By Dr. J. Russell Smith, Professor of Industry, University of Pennsylvania, Philadelphia, Pa. 5. Open Discussion of the Problems Presented. AFTERNOON SESSION Wednesday, February 21, 2 o’clock, P. M. 1. Presentation of the Report of the Committee on Resolutions, 2. General Discussion, Adjournment. (13) 14 In addition to the above stated papers on the advance pro- vranune, others were read or formally presented as follows: 1. A paper on the “Botanical History of Diaporthe parasitica and Allied or Identical Fungi,’ by Prof. W. G. Farlow, of Harvard University ; read by Prof. G. P. Clinton. 2. A paper on the “Relation of Insects to the Chestnut Bark Disease,” by Dr. A. D. Hopkins, of the Bureau of En- tomology, U. S. Dept. of Agriculture, Washington, and read by him. 3. A paper entitled “Chestnut Blight and its Possible Remedy,” by Mr. W. M. Benson, of the Oak Extract Company, Newport, Perry Co., Pa. ~ 4. A paper entitled “The Field Work of the Chestnut Tree Blight Commission,” by Thomas KE. Francis, Field Su- pervisor of the Pennsylvania Chestnut Tree Blight Com- mission, 5. A paper entitled “A Report on Scout Work on the North Branch of Bald Eagle Mountain, between Sylvan Dell and Williamsport, Lycoming county, Pa.,’ by Hugh E. Wells, Field Supervisor of the Penna. Chestnut Tree Blight Commission. Conference for Preventing the Spread of the Chestnut Tree Bark Disease. OPENING SESSION Tuesday, February 20, 1912, 2 o’clock, P. M. CALL TO ORDER AND ADDRESS OF WELCOME TO DEL- EGATES AND VISITING FRIENDS, BY THE HON. JOHN K. TENER, GOVERNOR OF PENNSYLVANIA. GOVERNOR TENER: Gentlemen, the meeting will please be in order. Let me say at the outset, speaking for this Commonwealth and less for myself personally, that we are gratified indeed at the splendid representation here to-day, bearing testimony to the ereat interest manifested in the work at hand. I know that many of you have come from afar, many of you at great inconvenience and certainly at expense to yourselves or to the State or Association that you represent, in order that you might meet with us here, in the Capital City of Pennsylvania, to discuss and to consider seriously the objects and the pur- poses of this meeting. | It is not my purpose to enter into an extended discourse upon the subject of the chestnut tree blight or bark disease, but rather to extend just a word of welcome to you, on behalf of our Commonwealth and our city, and also to suggest what might be proper for your consideration at this time; to go over briefly the extent of this disease in the area it now covers; what it means to us if it spreads farther, and what it has meant to us; the value of our chestnut trees, and a suggestion of what I hope (15) 16 you may be able to arrive at before you leave us.. We know that in conventions, we cannot exercise any governmental function; yet we want this to be something more than a “resolve to re- solve” meeting, and we hope that something really tangible will result from it. I have noted just a few things which, as I stated before, I would like to have you consider in your delibera- tions: This Conference has been called for the purpose of obtaining all possible information concerning the best methods of fighting the destructive fungous disease known as the chestnut tree bark disease or the chestnut tree blight, which was first detected in the neighborhood of New York City about eight years ago, and has since spread to the Northeast as far as EKastern Massachu- setts, and to the Southwest as far as Central Pennsylvania, Maryland and Northern Virginia. This tree disease is virulent in character. To date, no specific remedy to be applied to individual trees is known. It seems almost unthinkable that a disease of this character should have invaded so large an area and that no means of pre- venting its spread is yet at hand. Unless this disease be stopped by concerted action among the States, it is certain that within a few years very few living wild chestnut trees will be found in America. It is, therefore, entirely in accord with the American spirit that we make every effort to destroy or check the advance of this blight. The value of the standing chestnut stock to-day in America is enormous. In Pennsylvania alone, the wild chestnut tree is found native throughout the State, and in its southern counties is the principal remaining forest tree. The value of this tree in the State of Virginia is reliably conceded by competent au- thority to be not less than thirty-five millions of dollars. I be- lieve that here in Pennsylvania, by a very conservative estimate, placing a valuation of fifty cents upon each tree in our wood- lands, which you will admit is a very low estimate, the value of the wild chestnut trees is at least forty millions of dollars. The best chestnut in the world is still standing in the moun- tains of North Carolina, West Virginia, Eastern Kentucky and Tennessee. The chestnut stock of the future must necessarily be drawn from these states. To date, the blight has not reached 17 that region, but is steadily tending in that direction. This tree is also of great value in Ohio and the remaining Atlantic Sea- board States, and by reason of the all too prevalent forest de- struction going on, the tree can ill be spared; much less its value wasted, as it largely will be, should the remaining chestnut stock be attacked. The destruction of the wild chestnut trees in New Jersey, in Southeastern New York, Western Connecticut and Massachu- setts and Southeastern Pennsylvania is marked to be complete. The industries depending upon the wild chestnut tree for their support are of large proportions and great value. Every part of the tree is valuable for making tannic acid, used in the tanning industry. Telegraph and telephone companies depend mostly upon this tree for their stock of poles. The railroad com- panies are largely dependent upon it for their best railroad ties. The nuts are no inconsiderable part of this valuable product. Many thousands of men are employed in the industries depend- ing upon the saving of the wild chestnut tree, and many other thousands of real estate owners will find their land values seri- ously affected should the tree ultimately be destroyed. Two great facts to be borne in mind are, first, that the plague is with us and it must be reckoned ‘with; and second that har- monious action and complete co-operation among all the inter- ests involved, as well as the governments of the various states, can and will be the only means of checking this disease, if it can be checked. We are not so much concerned with its origin as we are with its presence and effects. While its botanical his- tory and pathology are of importance, the real thing is prepared- ness to repel the invader, using every means known to science and practical experience. It is, therefore, to be hoped that this aspect of the problem will be thoroughly taken hold of and discussed from every point of view, that concerted action will be immediately inaugurated, and no effort left unemployed that might produce desirable re- sults. The time to act is now, and not after the scientific world has more fully worked out the history and pathology of the dis- ease. Present day practical measures may well be aided by scientific inquiry, but the one by no means must wait upon the 9 ~_ 18 other. It was because of Pennsylvania’s realization of the im- portance of this work that the Legislature, at its last session enacted a bill creating a commission and defining the duties of that commission, as well as appropriated an adequate amount to carry on the work. Without reviewing that bill in its full text, it might be said that the proposed Commission was given the direction to seek out and destroy this disease. As Admiral Dewey, you remember, at about the outset of our war with Spain was directed by President McKinley and the Cabinet to seek out the Spanish fleet and destroy it, so it might be said that the only direction given this Commission was to find this dread chestnut bark disease, and destroy it. That Commission has been organized, and this State is in- deed fortunate in being able to command the services of such splendid men, such capable men as Messrs. Sargent, Peirce, Craig, Bodine and Ely, who have gone about their work with the determination to do all that is possible to bring about the de- sired results. Were the cause of this disease known, and did we know how to combat it and how to destroy it, a meeting of this kind would be unnecessary; but we do know something of its ravages, how it attacks the trees, and now we are here to consider how we shall blot it out; how we shall arrive at the source of it, if pos- sible, and then blot out the disease completely. I am prepared now to consider a motion looking to a proper organization of this convention for the carrying out of its work, and for the proper recording of your deliberations to-day. MR. HAROLD PEIRCE: I would nominate, as permanent chairman of the Conference, Dr. R. A. Pearson, former Commis- sioner of Agriculture of the State of New York, and as secretar- ies, Messrs. F-. W. Besley, of Maryland, and Samvel B. Det- wiler, of Pennsylvania. THE GOVERNOR: You have heard the motion. The ques- tion is upon the election of Mr. R. A. Pearson, former Commis- sioner of Agriculture of the State of New York, as chairman of this Conference, and Messrs. IF’. W. Besley, of Maryland and 8. B. Detwiler, of Pennsylvania, to serve as secretaries of this Con- ference. The motion was put and unanimously carried. 19 THE GOVERNOR: Mr. Pearson is unanimously elected chairman, and Messrs. Besley and Detwiler are unanimously elected secretaries. I would suggest, gentlemen, for the com- plete organization for the transaction of your business, that some one be selected or designated to report the proceedings of this convention. MR. I. C. WILLIAMS: I suggest the name of Mr. Victor G. Marquissee, who is here prepared to report the proceedings of this convention. THE GOVERNOR: Without objection, the gentleman named in the motion will report the proceedings of this Convention. I now take very great pleasure in presenting to you, and calling to the Chair, the Chairman whom you have elected, Mr. Pear- son, of New York. (Applause). Mr. Pearson took the chair. THE CHAIRMAN: Governor Tener, Ladies and Gentlemen: I appreciate that it is a great honor to be asked to preside over your deliberations. I accept the honor, and thank you for it, with appreciation also that it carries with it great responsibili- ties, for this is an important Conference. It is important be- cause of the great commercial interests involved, and it is also im- portant because of the intricate scientific questions that are involved. That its importance is well recognized could not be better shown than by the fact that the Governor of this great Commonwealth has called this Conference together, that it meets in these splendid quarters, and that this State has taken the lead in providing for practical, efficient work to be done in checking the ravages of the chestnut blight, through the efforts of a special Commission, the competency of the members of which is recognized not only in your State, but in many other States as well, where the work which they have begun has come to be known. Four months ago we held in the Capital city of New York, a Conference of much smaller proportions than this, but called together to consider the same questions; and at that time we were told that it was the purpose of Governor Tener to call this larger Conference, and we have been looking forward to this time as an epoch-making event. 20 It has been suggested that we should do nothing to counteract the ravages of the chestnut tree disease, because we are not fully informed as to how to proceed. That is un-American. It is not the spirit of the Keystone State, nor the Empire State, nor the New England States, nor the many other great States that are represented here, to sit down and do nothing, when catastrophies are upon us. It has been suggested that we should wait patiently until the scientists have succeeded in working out these ques- tions in all their minutiae; that thus we may be able to accom- plish our results more quickly. But that is not the way that ereat questions are solved. If we had waited until the appli- cation of steam should be thoroughly understood, we would be still waiting for our great trains and steamboats, which are the marvel of the age. (Applause). We know some things about this curse, and we are here to exchange ideas; to tell, on the one hand, what we have learned through our scientific studies, and, on the other hand, what we have learned through our practical work; and thus we believe that at the close of this Conference, we will all go away from here, wiser and better prepared to carry forward the great work in which we are interested. Now we are here for business. The Governor has given us the keynote for the meeting. I should not take your time further in making remarks, but let me say to you that, so far as in me lies, these meetings will be expedited; they will begin on time; the programme will go forward without unnecessary delays; and I only ask that the Chair may have the sympathy and the cordial co-operation of the many delegates who are attending the meet- ings, to the end that when we close, we may all feel that it was well that we came together. Unless other arrangements are made, the Chair will understand the usual rules of procedure will govern our deliberations, and he will follow those rules to the best of his ability, being always willing to be corrected or to be overruled by those who are participating in the Conference. The Chair will now recognize Mr. Samuel T. Bodine, of the Chestnut Tree Blight Commission. MR. BODINE: In order that the deliberations of this Con- ference may be properly summed up, Mr. Chairman, I move that a Committee on Resolutions be appointed by the Chairman 21 of this Conference, of which he shall be a member ex-officio, which Committee shall be representative of the various States inter- ested in the wild chestnut, and represented at this Conference. The motion was seconded. MR. 8S. M. ENTERLINE, of Pottsville, Pa: I would further add, Mr. Chairman, that these proceedings should be reported and printed, if that be possible, and forwarded to the delegates, as far as the supply of reports may reach. THE CHAIRMAN: That question may come up properly a little later. The motion now before you is on the appointwent of a Committee on Resolutions. The motion was put and unanimously carried. THE CHAIRMAN: The Chair will be pleased to receive, if the opportunity offers, suggestions from members as to their de- sires in this or any other matter. The programme now calls for brief responses to the Goyernor’s address, and it has been suggested that the best manner of pro- cedure will be to call the roll of States which are represented here, asking one person from each State to make a response; and, in order that we may get through the list promptly, unless directed otherwise, the Chair will have to ask each State to limit its response to three minutes. It may be that some of the first names on the list are not prepared to respond at once. In that case we will pass them over and return to the names a little later. Alabama. (No response). Connecticut. DR. GEORGE P. CLINTON, New Haven, Conn., Expt. Sta- tion: Mr. Chairman: I hold a commission from the Governor of Connecticut to represent that State, with two other delegates, at this Convention. In Connecticut we have studied this disease somewhat longer than you have here in Pennsylvania, and we have it in a very serious manner. I am not officially on the pro- gramme, but I have prepared some of my ideas and views on this subject which I wish, at the proper time, to present to this Con- vention. Ihave also a paper by Professor Farlow, from Harvard University, who has studied the history of this fungus, that I wish at the proper time to present to the Convention for their 22 consideration. I take it that we want in this Convention, to know everything that is known concerning the chestnut blight and from that to deduce our conclusions. In that respect 1 am prepared to present all that I know and my views on the subject, in order that the truth, if such is known at present, may come out. THE CHAIRMAN: The District of Columbia. This in- cludes the Federal Department of Agriculture. Is Professor Collins in the room? PROFESSOR J. FRANKLIN COLLINS, Department of Agriculture, Washington, D. C.: I am not prepared to make any remarks for the District of Columbia. I come from another direction. JT have no remarks to make particularly. THE CHAIRMAN: We will give you an opportunity later, Professor Collins. The Dominion of Canada. (No response). Delaware. Dr. WESLEY WEBB: Mr. Chairman, Delaware sends a delegate up here to learn the situation. Delaware itself is pretty thoroughly infested with this disease. Every chestnut growth and every forest has diseased trees in it. The only way to destroy the disease in Delaware, in my opinion, is to destroy every chest- nut tree and clean it up. I doubt if any measures short of that would be successful; but still, something may be learned here that will modify that opinion. THE CHAIRMAN: Georgia. (No response). Illinois. (No response). Indiana. (No response). Maryland. MR. J. B.S. NORTON: Mr. Chairman, I had supposed that Professor Patterson would speak for our State, as he is inter- ested from the forestry standpoint, and I am interested in the Jxperiment Station from the nursery standpoint. We will have a problem to meet in our State in controlling this disease, and I am sure we are very actively interested in this work, because we are in the same condition as a few other States. We have a large part of our area already infested, and a considerable part 23 of it that is free, so it makes it a more active and important ques- tion to us than to sections where the territory is entirely covered with the disease. THE CHAIRMAN: Massachusetts. PROFESSOR EF. W. RANE, State Forester: Mr. Chairman, I was sent out here by Governor Foss. I had an opportunity to have a conference with the Governor shortly before coming. We had hoped to bring along some of our large timber owners, but, at the last moment, it was impossible to make arrangements. The Governor said it would be impossible for him to be here, but urged me to extend his compliments to you by all means. In Massachusetts we are just beginning to realize that the chestnut bark disease is a very serious menace to us. During the past year we have had a man from the Department of Agriculture with us for three months, and I have had all my assistants in the State Forestry Department out in the field hunting it down. We find that it is scattered pretty much over the State. Our simple remedies we send out by men that are with us, and we are always ready to assist anybody in any part of the State with any sug- gestions possible in regard to it; but I do not care to talk about that at the present time. Iam here to learn everything possible, and am glad to be here, I assure you. THE CHAIRMAN: New Jersey. DR. MELVILLE T. COOK: Mr. Chairman, in the State of New Jersey I find, although I have been there but a short time, that those who have looked into the situation most carefully are inclined to believe that, so far as the State is concerned, the situ- ation is practically hopeless. Almost every chestnut growth in the State is infected at the present time. We expect, of course, to do some work in combating the chestnut blight, because we will not give up until the chestnut timber is entirely destroyed. While the majority of those who have been making a study of the conditions over the State look upon the situation as hopeless, yet we can say that there has some good come out of evil, because at the present time the people are wike-awake to the importance of the careful study of plant diseases. At the present time there is no difficulty, whatever, in getting the people to listen to any 24 advice that looks toward the protection of the natural interests of the State. So the State of New Jersey greets the Convention here to-day with honest hopes that something may be accom- plished which will advance the public interest and welfare. THE CHAIRMAN: New York. GEORGE G. ATWOOD: Mr. Chairman, the State of New York appreciated very highly the honor extended by the invita- tion of the Goyernor to be here to-day, so as many as possible of the delegation accepted with pleasure. We are here to-day to learn something in order to perfect a plan that has been brew- ing in New York State. New York State has a large chestnut area to save. We have a small section of the State where the chestnuts are practically gone. Arrangements are being per- fected for carrying on the work under the advice of the botanists of our stations, and we hope soon to have a forest plant patholo- gist, working either with the Department of Agriculture or with the Conservation Commission. The Governor of the State is very much interested in this proposition. We are waiting for some definite plan, which will be taken hold of as quickly as it can be devised, and as thoroughly as the necessities of the case re; quire. THE CHAIRMAN: North Carolina. (No response). Ohio. DR. AUGUSTINE D. SELBY: Mr. Chairman, Ohio is very much interested in this Conference, because Ohio lies in the western part of the Appalachian chestnut belt, and, as State Pathologist, the problems of the chestnut bark disease would be- come our laboratory and field problems. As yet we are not aware that the disease exists in Ohio, although it may be so; but we are perfectly aware that our success is indissolubly bound up with the success of Pennsylvania and the states to the east of it. If Pennsylvania, either by reason of a natural change in conditions by which the parasite of this chestnut bark disease becomes less virulent, or by the trees becoming more resistant, is not able to save a portion of its chestnut growth, then Ohio will not be. If, on the other hand, Pennsylvania and West Virginia, as well as New York, are able to save their trees from the wrecking of this disease, then Ohio will realize the advantages of such a Confer- or av ence and such work. I assure you that whatever efforts are made by this Conference, or whatever conclusions are reached by this Conference and whatever efforts are made by other States, these will be supplemented with vigor in our own area. Personally, of course, We are without experience in the disease. For ourselves, we feel that we have in the chestnut bark disease one of those occasional and epoch-making parasites which has arisen from the unknown and wrought incredible damages; that it will continue its aggressiveness through a long period may or may not prove to be true. If it prove to be true, then our difficulties are very, very great. If the conditions prove more favorable, our forests may be preserved. THE CHAIRMAN: Rhode Island. JESSE B. MOWRY, State Forester: Mr. Chairman, in be- half of the State of Rhode Island and the other delegates repre- senting that State, I desire to acknowledge the very cordial wel- come extended to us by the Governor of Pennsylvania. Last sum- mer a systematic inspection of the State of Rhode Island was made, under direction of Professor Collins, and this disease was found to exist in the chestnut-growing portions of the State. We are very glad to be here, to learn what we can about it, and to profit by the pioneer work which the State of Pennsylvania is doing in behalf not only of its own Commonwealth, but in the interest of all the other States which grow the wild chestnut tree. THE CHAIRMAN: Tennessee. (No response). Vermont. (No response). Virginia. MR. GEORGE B. KEEZELL: Mr. Chairman, on behalf of the General Assembly of Virginia, I desire to return thanks to the Executive of this great Commonwealth for the invitation to be present on this occasion, and to take part in these delibera- tions. So far as Virginia is concerned, we are at this time per- haps fortunate in the fact that, if we have this dread disease with us, we have so far had very little complaint of it. We are not here to give any experience of our own which may be helpful to others, but to learn from others what may be of benefit to the whole 26 Commonwealth of Virginia. As was suggested by the Governor in his remarks, a great deal of the wealth of the Commonwealth of Virginia is in our chestnut timber interests. Within the last decade her chestnut timber has been the source of a great deal of income to Virginia, and of a great deal of wealth. Its more re- cent use, for tannic acid, has brought into great value the waste places of the State, and timber heretofore regarded as not very valuable has become one of the most valuable assets of the Com- monwealth. Naturally, we are very much interested in anything that goes toward the preservation of this valuable timber, and at this time we are especially grateful for the invitation to be here, because our General Assembly is now in session, and bills have already been introduced looking toward appropriations to com- bat this disease; and we are particularly anxious to get all the in- formation we can here, in order that we may go back and give our legislators the necessary facts. I have no doubt provisions will be made by the Commonwealth to fight the ravages of this dis- ease. THE CHAIRMAN: West Virginia. DR. N. J. GIDDINGS: Mr. Chairman and Ladies and Gentlemen: I can assure you that the people who are most in- terested in West Virginia appreciate the opportunity which this Commonwealth has offered for meeting here and considering matters in regard to the chestnut bark disease. The chestnut in West Virginia is a very important tree. Just recently I learned of shipments from one station amounting to one hundred and fifty-five thousand pounds of chestnuts,—the wild nuts,— during last fall, and there may be other shipments that run as high, or higher. The annual cut of chestnut in West Virginia for the last two years has been about one hundred and eighteen million feet, and has neither increased or decreased; but the disease is present in the State. To what extent, we do not know. We are in hopes to have at least one or two men in the field this spring to learn more in regard to the conditions in the State, and we hope to be in a position, after getting the details which we may from this Conference, to go back and undertake the work in.a much better manner than we otherwise could. 27 THE CHAIRMAN: For the Dominion of Canada, the Chair will call on Dr. H. T. Gussow, of Ottawa, the Dominion botanist. DR. GUSSOW: Mr. Chairman: On behalf of the Depart- ment of Agriculture of the Dominion of Canada, I am here to thank you for your very great courtesy in asking us to partici- pate in this very important meeting. I may say that, as far as we are concerned in Canada, we have not this dreaded disease at the present time, and we have been very anxious to ayoid the im- portation of it across the border, by passing stringent legislative measures prohibiting the importation of chestnuts of any kind, nursery stock or even chestnut wood, or anything else connected with chestnuts. I find that this will probably be the only means to restrict the disease to the States in which it is found at the present moment, and I can only extend to you, neighbors of the United States, my best wishes to succeed in combating, or at least, restricting this very serious disease. THE CHAIRMAN: Are there other States represented who have been passed over? Are there any States we have not heard from? MR. J. W. FISHER, of Tennessee: Mr. Chairman, we are very greatly interested in this subject, because we have such a marvelous growth of chestnut in Tennessee. It is receiving very considerable attention at the present time from the axemen, for lumber and tannic acid. It has a vital connection with our water sources, because it covers the area so completely that if it were destroyed, it would vitally affect vast water powers and irriga- tion. We are therefore, extremely interested that you, in your deliberations, should find some means of checking this disease, that we may have our forests preserved to us. I shall take a great deal of pleasure in reporting whatever I can to our Goyernor, Hon. Benjamin Hooper, whom I have known for years and who comes from our town, so I think I am in an attitude to bring the attention of the State to this matter, and I shall be extremely glad to do so. THE CHAIRMAN: Are there still other States represented, who have not been heard from? A number of delegates are ex- pected in later in the day. You will all agree with the Chair: ‘ 28 when he suggests that it is very much like having the play of Hamlet with Hamlet left out, when we fail to hear from the great State of Pennsylvania; but, as usual, this State asserts her modesty, and has insisted on being excused for the present. The Chair will assure you that later we will hear from the State of Pennsylvania, and from more than one person. Unless it is otherwise decided by motion and vote, the Chair will request that all resolutions be handed in at the desk, without taking the time of the Conference to read them, to be referred directly to the Committee on Resolutions. This, how- ever, may be overruled if the delegates desire to take the matter into their own hands. | | T am informed that provision has been made for registration at one of the ante-rooms outside of the entrance to this chamber, and each one is earnestly requested to register his name, home ad- dress, official position, and his temporary Harrisburg address. The program now calls for an address upon the ‘Historical Review and the Pathological Aspects of the Chestnut Bark Dis- ease,’ by Dr. Haven Metcalf, of the United States Department of Agriculture. It is with the greatest regret that we have learned of the serious illness of Dr. Metcalf, which makes it im- possible for him to be present at this time. Fortunately, however, we have with us Professor J. Franklin Collins, the Assistant Pathologist in the Federal Department of Agriculture, and Pro- fessor Collins has kindly consented to address us at this time. ADDRESS OF PROFESSOR J. FRANKLIN COLLINS, OF THE DEPARTMENT OF AGRICULTURE, WASH- INGTON, D. C. Mr. Chairman, Ladies and Gentlemen: It is with very great regret, for many reasons, as you can imagine, that « have to take Dr. Metcalf’s place here. I came here rather unprepared to take his place. The accident to Dr. Metcalf occurred on Satur- day night, and I had the chance to see him only a little while on N. No. 35. Distribution of the chestnut Bark disease. Horizontal lines indicate approximate distribution of uninfected chestnut; dots indicate isolated infected spots; the heavier lines in various directions indicate varying degrees of infection culminating in an area about New York City in which all chestnut trees are dead. > “ > { * * © 7 7 ae oa) i i a 7 No. 1. Branch of a chestnut tree showing a disease lesion on smooth bark. No. 2. Portion of a branch of chestnut tree, exhibiting a lesion started around dead stub, the pustules being especially prominent. No. 4. Surface section of chestnut bark, with pustules in the crevices. Lower illustration shows pustule greatly enlarged, fiom which three spore threads have been produced. 29 Sunday. I have come here without many of his ideas. However, he has some slides which are to be shown, and perhaps I can tell you something about those, and so add to their interest. Before the slides are shown, L want, very briefly, to give a short sketch of the history of this disease. It will be very brief, and of a general nature only. The history of the disease has already been published in quite a number of cases, so [ will touch only upon the main points. Our attention was first called to this disease, I believe, in the fall of 1904 by Dr. Merkel, of the Bronx Zoological Park, in New York city. He noticed that chestnut trees were dying in greater numbers than seemed to be warranted by any previous knowledge of the dying of chestnuts. He looked the matter carefully over, as [ understand it, and decided that there was a definite disease there, and later turned the material over to Dr. Murrill, of the New York Botanical Gardens. Dr. Murrill studied this disease and later published his findings upon it, naming the fungus which caused the trouble, Diaporthe parasitica, a new species of the genus. At that time, I believe, Dr. Murrill stated that it was a very serious disease, and sent out a warning to that effect. If 1 am misquoting him, I hope he will correct me, for he is in this room to-day. It was not until 1907, three years after the dis- covery of this disease, that a laboratory was established in Wash- ington for the study of tree diseases. Since that time—almost immediately and since then—certain investigations, both in the laboratory and in the field, have been carried on in Washington, I do not propose to say anything about these studies at the pres- ent time. My point here is to give you a general idea of the disease, what it looks like, how it affects a tree, and things of a general discussion of the topic. This review will that sort, be, will necessarily have to be, primarily an explanation of the views which will be thrown on the screen. I may elaborate at points, but, as I say, I am not primed as Dr. Metcalf would have been had he been able to be here. I think perhaps we may as well proceed to the views at once. Slide No. 1. This, to begin with, shows a diseased spot, as we will find it on the smooth bark of a branch of a chestnut tree, a branch which is perhaps anywhere from three to six inches in diameter. The disease is a fungous disease, and starts its 30 growth from a very miscroscopic, one-celled body, which we know as a spore. By some means the spore reaches a place in the bark of the chestnut, where conditions are favorable for its growth. Its growth is not essentially different from that of the spores of other fungi. It consists mainly, or principally, of a threadlike growth coming from the spore. This threadlike growth branches, and finally we have a great mass of threadlike fila- ments. In the case of the chestnut disease, the spore may gain entrance at some point, say here, or some little break here, pos- sibly (indicating on slide), and perhaps occasionally without any break at all in the bark. The growth in the bark continues to in- crease in size, that is, the general area of the growth, and sooner or later, the same as in practically all plants, we have a fruiting stage of this fungus. This view shows some of these fruiting stages, as we ordinarily see them on the chestnut. Some of the stages, which are not quite so common, will be shown a little later; but I want to call your attention to the fact that, from this point to the point away over there (indicating) we have an area of disease. As a rule the bark in the smooth-barked limbs is somewhat sunken, where the limbs are two or more inches in di- ameter. Where they are below that diameter, the diseased area may be an enlargement rather than a depression in the bark. These little yellowish spots which you see all over here, many of them, are smaller than the head of a pin. They are of various colors, but usually some tint of yellowish brown or orange, or sometimes they weather to a darker color. Those pustules are what we know as the fruiting pustules of this fungus. These pustules, during the growing season, in the summer as a rule, produce a certain type of spore, and later in the season, or at a later stage in the age of the disease, at least another type of spore. Tor convenience we will speak of the first type as the summer spores and those of the later stage as the winter spores. No. 2. This shows a similar branch with a lesion, which has started evidently from around this old dead stub, and this has spread until we get the diseased area from this point, from here probably, (indicating) up to the top of the picture. Now dur- ing the summer, or rather after a rainy spell which is followed by a dry spell, perhaps two days or one day or three days after the rain has ceased, we shall find that these pustules, or fruiting 31 spots, have pushed out a little mass, a threadlike mass, in much the same way as you would press out the paste from a collapsible tube by pinching the tube. As a result we get, perhaps, from one of these pustules, anywhere from one to fifteen or twenty structures of that sort, (indicating) which are, of course, here greatly magnified. This represents the pustule at the base, this yellow area; and this is one of the threadlike masses which has been forced out by the swelling of the mucilaginous matter in the pustule. No. 3. Each one of those masses shown at the right hand side of the view is composed of many hundreds of thousands of spores, no larger than bacteria. One of these spores may, so far as we know, under favorable conditions, reproduce this fungus and con- sequently reproduce the disease, if it starts growth in the proper place. No. 4. This shows simply a somewhat larger view of one of those pustules, from which three of those spore threads have been produced. At the upper part of this picture we have a sur- face view of the chestnut bark in which we find the pustules gathered in the crevices. This is rather characteristic on chest- nut bark that is of a sufficient age to be cracked. Only on smooth chestnut bark, as a rule, do we find these pustules all over the bark. In the cracked bark we find them primarily, if not entirely, - in the crevices. No. 5. There we have a section of a small branch that shows some of these pustules, and above some of these threads as they appear on the bark of the chestnut. I have nothing special to say about that view, except that, so far as the color is concerned, we are apt to get it just that color, but quite as often somewhat darker, with a little orange or reddish tint to the pustule. No. 6. Now if we take one of those areas of disease on smooth bark and cut into it, if we shave the top of the bark off with a sharp knife,—suppose we take just such a case as we have at the left here (in fact this is made from the same branch) and shave it so as to show what is beneath,—we get a discolored area, a rather characteristic area, which is not shown as well in this view as it will be in another; but remember that this view at the right represents such a branch as that at the left, with the sur- face of the bark removed with the knife. 32 No. 7. Here is a view which represents a branch, from which the surface of the bark has been shaved in the same manner as in the last view, but here we have the characteristic fanlike mott- ling, which we often get in the bark beneath the surface. Some times the effect which you see here is produced immediately be- neath the surface of the bark, at other times down in the middle of the bark, and at other times you have to get in pretty well to- wards the wood in order to find this characteristic marking, de- pending largely upon whether there is a perfect epidermis, or perfect skin, over the bark, or whether there is a corky layer; but it is not entirely gauged by those characters. This line (in- dicating) representing the line of discoloration; the infection started at this point and radiated in all directions from the com- mon starting point. Of course, if we shaved off the other side of that branch, we should have expected to find about the same condition of affairs there; but here we have shown only the half circle of the more or less circular area of the disease. No. 8. Here are two branches of a chestnut tree, an orchard tree as I recall it. These branches are about four or five inches in diameter. This represents a very common appearance on chestnut in the smooth-bark stage. Of course, this has begun to crack more or less from age. That is not an exceptional case by any means, as all who have seen the disease will readily realize. ' No. 9. This represents another case of a diseased portion, in which the disease started about at this point (indicating). One of these cracks probably represents the position of the starting point of the disease, and it has radiated in all directions, tending to form the circular mass which is shown here, running down there and across the bottom and of course off of the view entirely at the right. That is a grafted tree, by the way, and the enlarged portion at the middle of the tree represents the graft line. No. 10. This is merely a section of a little older piece of bark, where we get the pustules of a darker color, that is, more of the brownish tinge, as we often do in weathered bark. This, as I said a moment ago, is found in material which has withstood the weather for some time. No. 11. This is another view which shows merely some of the older pustules. This is intended more to represent the winter stage of the fungus. I do not think, however, that you will be No. 6. Sections of smooth-barked chestnut twigs showing disease lesions. Sur- face of bark removed from right-hand specimen, showing discolored and diseased areas. No. 7. Characteristic fan-like mottling revealed by shaving the bark of a diseased branch. No. 8. A large area of disease pustules on a smooth-barked orchard tree.—Photo- graph by Prof. Collins. No. 9. Bark removed from over a canker, showing the cracks at the centre and the fan-shaped spread of the yellowish fungous mycelium; also, at the lower edge, the circular margin of the disease.—Photograph by Prof. Collins. able to make out the individual little spots which go to make up one of these common masses. The winter stage of this dis- ease produces its spores down in the bark; that is, down beneath the surface of the bark, and so also does the summer spore stage, except that in the summer spore stage they are extruded in the form of these threads, while the winter spores are not extruded in the same way, although they are extruded later. No. 12. This view represents a diseased spot on an orchard tree. The diseased spot is less than three years old, but more than two years old, according to the records which were kept. This shows, at the upper part of the picture, how the bark soon loosens and later falls from the tree and the branches, until finally we have simply the bare trunk or a bare branch left. Sometimes this bark breaks away in less than two years, to much the extent that is shown there. No. 13. Here is a small twig of a chestnut. A little while ago T mentioned the fact that, in the smaller twigs, we sometimes had an enlargement when the disease was present, rather than a depression. flere at the left we get the normal size of the twig, and then, running out this way towards the apex of the branch, we see where the disease started, and we have this considerable swelling. This is quite characteristic, under certain conditions, of twigs which are less than a half inch in diameter. It some- times occurs in larger branches, but as a rule we get it quite com- monly in this type of branch. No. {4. In the older trees, where the bark has become deeply furrowed, I said that we found the diseased pustules almost en- tirely in the cracks or crevices of the bark. This represents the surface,—egreatly magnified, of course, and beyond what you might imagine,—and some of the furrows. We get the yellowish- orange pustules in the crevices there, and in various places, whereas the other parts, the raised places, show no pustules at all. No. 15. So much for the disease as it appears on the branches. Now when the disease appears on a branch, or on the trunk of a tree, it starts from the common point and radiates in all direc- tions, forming the more or less circular area of disease. Of course, on the trunk of a tree it goes up the trunk from the com- 3 o4 mon point, down the trunk, and around the trunk. When these portions of the disease which go around the trunk meet on the other side, we have a branch or a trunk which we speak of as eirdled. Now a girdled branch, or a girdled twig, or a girdled trunk, means the early death of all parts of the tree beyond the girdled area. If it is a twig, it means the death of the twig be- yond the girdled area. Jf it is the trunk, it means the death of the whole tree at once, or soon after the girdling is completed; not immediately, as a rule. Now I want to call your attention to some of the obvious effects of this girdling upon the foliage of the tree. When you are looking for this disease during the sea- son of foliage, it can be detected oftentimes at a great distance. I have myself detected diseased trees more than a mile away, or trees supposed to be diseased, by the characteristics which I want to call your attention to now. ‘To be sure, you must bear in mind that the coloration of the leaves to which I am going to call your attention can at times be brought about by other things than this disease; but we have in the coloration of the leaves, as we gener- ally say, the “danger signal” which suggests where to look for the disease; for, if the disease has been going on very long, for a few months, or weeks even, in certain places, we shall get some of these discolored leaves as the result of the girdling of some one or more of the twigs or branches. I have shown here a somewhat normal chestnut leaf. It is a little broader than the normal leaf; this is intended to represent not, perhaps, a perfectly typical chestnut leaf, because we have on the margin a little paler green than in the portion in the centre. The pale green in the margin of every leaf at times, is one of the first symptoms of discolora- tion. It becomes a little pale. First of all, perhaps, the leaf wilts a little, if you notice it carefully, and if this paleness of the leaves is extended over the leaves of a whole branch, the effect as a whole is quite noticeable. No. 16. Here is a greenhouse plant which has been inoculated with the disease. At the left we find some of the normal chest- nut leaves; at the right a branch which had been inoculated and has been girdled way down here. (Indicating). Now I do not know about that particular specimen, but, if we were looking for the disease on such a specimen as that, we should never look up here for ‘it; that.is, not primarily. What is causing the trouble No. 10. Bark showing pustules of a dark color or of a brownish tint, due to longer exposure to weather.—Photograph by Prof. Collins. No. 12. Diseased ches‘nut tree showing shredded bark after two or three years in- fection.—Photograph by Prof. Collins. Live S oo i & } a O No. 13. Small twig of chestnut with enlargement due to disease. At the left side the normal size of the twig is shown. No. 15. Normal chestnut leaf. A pale green in the margin is one cf the first symptoms of discoloration and disease. oo with that stem is down here somewhere, down below all these dead leaves. That applies to looking for the disease on the tree, ov on the sprouts or suckers which may come up from the base of a tree. No. 17. In very young nursery stock, or the young sprouts which come up from a tree, or the vigorous growth on a tree, on the twigs at least, we often get this type of the disease at its very beginning. This is often more brilliantly colored than shown in this view. It is very conspicuous indeed, particularly on nursery stock. Although the view does not show any fruiting pustules at all, by cutting into that area we get the characteristic mottled mycelium or vegetative stage of the fungus beneath the bark. No. 18. Now we have a branch which shows the withered and yellowish leaves. This yellow color follows along after the pale green color. It is not a pure yellow, as a rule, although some- times it has been quite strikingly of a pure yellow color. You will notice that the leaves wither after awhile; that is, they crumple up after a time and that crumpling is shown, to a certain extent, in this view; and also the yellow color. No. 19. A little later we have the deeper color. This shows the browner coloration around the margin of the leaves. At the left we have two leaves which show merely the beginning of the discoloration. At the right the leaf is somewhat crumpled, bent, and discolored. No. 20. This is a stage much the same as that of the little branch which was shown three views back, this showing a larger view of the same thing. No. 21. Finally the leaf assumes a somewhat brownish tint, which is shown here. The leaves in this condition are often more crumpled and curled up than shown here. These two leaves have been flattened out somewhat so as to show the color. No. 22. Now to take some of the woodland views, to show how the disease looks in the landscape. Here is a large tree which, owing to lack of special instruction as to the coloring of it, lacks one or two features which it ought to have. For instance, this branch up here, and that whole branch (indicating), ought to have shown the yellow brown color. The coloring, however, was not noticed in time to give instructions in regard to it. This view, however, is shown primarily to represent the type of tree 56 Which is so valuable in the large estates in the various States. This particular tree had a circumference, above the settee which is there, of more than nineteen feet. The view was taken three years ago. That tree now has only two or three of the green branches left and the whole top of the tree is cut off. IT am sorry I do not have the other views to go with this, but through some slip somewhere they were not forwarded to be shown. No. 25. Now we have a view in which the disease has a start up in this corner, and the discoloration of the leaves, or the Inasses Of leaves, is here shown. Now a discoloration of this sort, particularly when it comes to a little later stage and has a more brilliant color, is quite conspicuous in the landscape. This view does not do credit by any means to the point which is intended to be brought out here. No. 24. Here is a view taken on Long Island, which shows the effect on the tree; a tree which has been nearly killed by the disease, showing the practically defoliated type of tree. Here is another type, (indicating), which has become badly diseased, and we have a bunch of sprouts appearing at this point, alse here, and also basal sprouts coming up. These sprouts are rather char- acteristic; perhaps I should not say characteristic, but they are commonly found connected with this disease, and are supposed to be more or less characteristic of the disease, but the sprouts can be produced by other means than as a result of the disease. No. 25. Another tree, also on Long Island, in which all but two of the lower limbs on the left hand side haye been killed by girdling from the disease, and now we have remaining only those two, or perhaps three, lower left hand limbs. No. 26. This is a tree showing the sprout growth which I alluded to in one of the last pictures, to even better advantage. Notice the sprouts which come up around the base, and the sprouts which come from the trunk at various places up in the crown. No. 27. There you have another type of the same thing, a more pronounced example, in which the sprouts are confined almost entirely to the trunk of the tree and everything is dead or dying, except perhaps one or two branches. No. 28. This view is shown in order to call to vour attention this particular tree (indicating), which shows four good lesions No. 16. A green-house chestnut tree in pot, three menths after artificial inoculation with summer spores. Photograph by Brewer. No. Uf Early effect of the disease upon young chestnut sprouts and nursery stock. No. 18. Characteristic withered and vellewish leaves on chestnut twig infected with the disease. -_ 37 of the disease, diseased spots, on the trunk of the tree. That is the way the tree looks when this disease attacks the trunk. That tree is practically dead. The lower part, represented by the lower half of that picture, shows some life. No. 29. In the course of two or three years we find that the bark begins to peel from the trunks of the trees. At the left we have a tree which las only recently been killed, that is, within a year or so perhaps, and the next one to it is one which is a little older, and the bark has begun to peel off. The one which is so prominent is probably the first in the group which was attacked and killed, and the bark has practically disappeared from the tree, so far as this view shows. No. 30. Now to consider the more general appearance of the woodland, here is a view taken in Forest Park, Brooklyn, along the Boulevard. This is one of the main boulevards through the Park, and any of you will have no difficulty in picking out the chestnuts. They are the most conspicuous objects. Not one of the green trees you see there is a chestnut. No. 51. Here is another view taken, I think, at Port Jeffer- son on Long Island. It may have been a New Jersey view; I am a little uncertain as to just where it was taken. That shows the young growth coming up and beconing diseased, and shows the effect along the hedgerow that we get from this disease. No. 32. This is one of the most southern stations which we know for the disease. This view was taken in South-western Vir- ginia, in Bedford county. The more prominent trees there have lost the bark entirely. Those trees, I understand, have been cut out and no longer exist. No. 53. If you want to see what the chestnut disease can do in a very nearly pure stand of chestnuts, there is a view which will show it. That was taken in Forest Park on Long Island. Any of you who have been in Forest Park will probably recognize that view. No. 34. The next view, I think, is another view of a little different portion of the same Park. These trees at the right are not chestnuts at all. This one up here, I believe, is a chestnut and there are some oaks there at the left. No. 55. T want to call your attention to the distribution of the chestnut, and, to do so, T want to call your attention to this map. 9 oc This map represents the eastern portion of the United States and the horizontal lines represent the approximate general dis- tribution of the chestnut tree. It may not be exact. I think most any of you who live at or near the border line represented here would have some suggestions to offer, but the map has been com- piled from as reliable general sources as we could obtain. Thus we have the chestnut from northern Mississippi, through northern Alabama and Georgia, northwestern South Carolina, western North Carolina, up through this region and up into the northwestern edge of Androscoggin county in Maine. In New Hampshire and Vermont there are only a few chestnuts present, as compared with the region farther south. Down through here (pointing to the southern Alleghanies), we have our great chest- nut stand, particularly on the western slope of the mountains. In the State of Connecticut a bulletin which was published with- in a few years stated that probably more than fifty per cent. of the forest trees in Connecticut were chestnuts. That was on very good authority, and I do not hesitate to quote it. In Rhode Island the chestnut is of a little less importance, but probably pretty nearly half of the trees in Rhode Island are chestnuts. The proportion further south I am not so well informed about, but we have the bulk of the heavy chestnut timber south of the Potomac River. The black area on the map represents the places where practically all the chestnuts are now dead, and the various forms of lines which are shown on the map represent varying de- grees of infection, until we come down to the line right here. (In- dicating). These vertical lines represent the approximate limits of what you might call somewhat general infection. The black spots which are shown there represent the outlying spots of infee- tion, so far as we knew them in December. Here is the line through Pennsylvania. The eastern part of Pennsylvania is pretty well infected with the disease, and the work now being done in this region, (indicating), will be told about a little later by someone who is better informed than I am. In closing this address, I want to read just a few words and, if we can have the lights now, I will finish in about two or three minutes. Having seen what this disease is and what it is doing, we now come to the question which, T take it, we are gathered here to No. 21. Leaves of the chestnut. showing brownish tint from effect of girdling by the disease. No. 22. Types of ornamental chestnut trees killed by thousands. Note the small, diseased branches. Scene near Philadelphia, Pa.—Photograph by Prof. Collins. No. 19. Leaves of the chestnut exhibiting discolorations and curling of leaves caused by the disease. No. 20. Curled and discolored leaves of the chestnut at an advanced stage of the disease. 39 answer as best we can: What are we going to do about it? That is the question. Three conditions lie open before us, as we see it: First: Do nothing; lie down and let the disease spread as far as it will, and destroy as much property as it can. It must be acknowledged that there is ample precedent for this course, us well as ample scientific support. Beyond question, this is the easiest thing to do. Second: Conduct scientific investigations of the disease, but make no attempt to control the disease until these investigations yield conclusive results. Such a course would unquestionably yield results which would be valuable in future epidemics of dis- ‘ase, but it would not save the chestnut trees at this time. The President of the Carnegie Institution, in a recent address, enun- ciated the principle that the results of scientific research must be stated in decades, not in years. We must investigate the dis- ease as thoroughly as possible, but investigation alone, without application, will not save the trees. Third: Investigate as thoroughly as possible, devote as much money as possible to research on the fundamental problems re- lating to the disease, but, at the same time, put into force im- mediately whatever measures against the disease appear to be most promising, recognizing clearly that there is not time first to prove absolute efficiency. I am informed that, as an immediate result of the recent burning of the Equitable Building in New York city, a special commission was appointed to devise better methods of fighting fires in the congested business section of New York. The appointment of the commission was necessary and will unquestionably yield excellent future results; but I notice that the New York Fire Department, went ahead and did its best to put out the Equitable Building fire, without waiting for the re- ports of any commissions. It appears to me that we are in much the same situation. The fire is burning too fast for us to wait for the reports of experiments which will take from two to ten years time to carry out. We must go ahead, using the best methods that we have, and leave the results to the future. (Applause). THE CHAIRMAN: I am sure everyone will agree that this talk has been both instructive and interesting, and we are par- ticularly indebted to Professor Collins for stepping in at the eleventh hour, as he has done, and favoring us so generously. : 4idve ‘ ~>* e 40 PROFESSOR SELBY: Mr. Chairman, would it not be proper for us to send, on behalf of this Convention, at this time, an expression of our sympathy with Dr. Metcalf in his serious accident? I move you that such an expression be sent by the Convention. Seconded by Mr. f. C. Williams. THE CHAIRMAN: Such a motion naturally would go at once to the Resolutions Committee, but the Chair is glad to make an exception in this case. Professor Selby moves that this Con- ference send a message of sympathy to Dr. Metcalf, with hopes for his speedy recovery. The motion was put and unanimously carried. THE CHAIRMAN: The Chair will appoint Professor Selby a Committee of One to prepare and forward the message. The next on the program is a paper entitled “Can the Chestnut Bark Disease be Controlied?” by Professor I*. C. Stewart, of the New York Agricultural Experiment Station. CAN THE CHESTNUT BARK DISEASE BE CON- TROLLED? By PROF. F. C. STEWART, New York Agricultural Experiment Station. Mr. Chairman and Ladies and Gentlemen: My views are so much at variance with what I conceive to be the sentiment of this Conference that I hesitated somewhat to present them. I feel like one throwing water on a fire which his friends are dili- gently striving to kindle. But a sense of my duty to the publie and, also, myself, impels me to proceed. I assume that you are all familiar with the method of control Which has been recommended, namely, the one which has been outlined by Dr. Metcalf and Prof. Collins in Farmers’ Bulletin No. 467, so I shall not take time to explain it. If you are not familiar with it, vou will become familiar with it before the close of this meeting. od. Complete destruction of trunks have lost their bark. Photograph by Prof. Collins. chestnut Scene trees in Forest in a ne arly Park. n pure stand. ar Brooklyn, Many New , ae bP ee | : : a ® Seta 1 ‘ F _ a _ - = t ‘ i 7 ot [ie a "oe Ty No. 99 25. Very early stage; infection of twigs in top of trees, at upper right- hand side. Lancaster county, Penna——rhotogiaph by Prof. Collins. No. 24. Type of diseased chestnut tree on Long Island, New York, showing characteristic sprouts.—Photocraph by Prof. Collins. a ey * a; Py Aan ip hae OE ¢ i ie kee ene parr See ee ee ee wos * ee. oe am, No. 25. Tree nearly dead from the disease. Only the two lower left-hand branches remain alive. Scene near Cold Spring, New York.—Photograph by Prof. Collins. No. 26. Chestnut trees on Long Island. New York, showing the effect of the girdling of the tree by the chestnut bark disease-—Photograph by Prof. Collins. A chestnut tree on Long Island, New York, with sprouts points on the trunk.—Photograph by Prof. Collins. at various ay Ve No. 28. The chestnut tree in the centre of the picture shows four well-developed lesions.—Photograph by Prof. Collins. No. 29. Typical group of dead chestnut trees. Note dead suckers on the trunks. From left to right:—the first trunk shows the disease less than one year old, (nothnig evident in this photograph); the second, an infection of from two to three years old; the third four or more years old: and the fourth about three years old. Scene near Brooklyn, New York.—Photograph by Prof. Collins. e Fark No. 30. Dead chestnut trees along a boulevard near Richmond Hill, New York. Note healthy condition of trees of other species—Photograph by Prof. Collins. No. 31. Dead and dying sprout growth. Note healthy condition of trees of other species. Scene at Port Jefferson, New York. Photograph by Prof. Collins. Lee aS een © 1 oe E i © S. = th - y ~ FR me ee re mee ie ns cols te pc No. 32. The most southern point of infection—a group of diseased chestnut trees at Fontella, Bedford county, Virginia. Photograph by Prof. Collins. No. 53. Complete destruction of chestnut trees in a nearly pure stand. Many of the trunks have lost their bark. View in Forest Park, neat Brooklyn, New York.—Photograph by Prof. Collins. 41 It is my opinion that we are rushing into this enormously ex- pensive campaign against the chestnut bark disease without con- sidering as carefully as we should the chances of success. The first question to consider is, can the disease be controlled by Met- calf’s and Collins’ method,’ of destroying advance infections and establishing an “immune zone?” This is a technical question of fundamental importance. It is a question to be answered by ex- pert mycologists and plant pathologists. I have observed that the leading advocates of the method avoid, as far as possible, dis- cussion of its probable effectiveness. In Farmers’ Bulletin 467, the question is disposed of by inserting into the letter of trans- mittal the following sentence: “The experimental data upon which the recommendations contained in this publication are based will be published in full in a forthcoming bulletin of the Bureau of Plant Industry.” The authors then go on to say (page 10) that “so far as tested” the method is practicable; and on page 11, after giving an account of what they consider a success- ful attempt to control the disease in the vicinity of Washington, D. C., conelude with the following statement: “It is therefore believed that this method of attack will prove equally practicable in other localities and if carried out on a large scale will result ultimately in the control of the bark disease.” Up to the present time the promised bulletin has not appeared and we are still in the dark as to the nature of the “experimental data.” I had hoped that it might be presented at this meeting. In justice to the public it should have been published before Bulletin 467, There is great need of some real evadence that the disease can be controlled. Apparently, the sole foundation for the optimis- tic statements made by Metealf and Collins in Bulletin 467 is the result of the field test? which they made at Washington and I hold that no definite conclusions can be drawn from that test. The chief criticism to be made of it is that there is no means of knowing what would have happened if the diseased trees had not been removed. There was no check, and experimenters are agreed that experiments without checks have little value. This is one of the first principles of experimentation. Weather con- ditions may have been unfavorable for the spread of the disease, 42 Most fungous diseases have periods of quiescence alternating with periods of activity, depending largely upon varying weather conditions. Also, there is reason to believe that the region covered by the test is not now as free from the disease as Metcalf and Collins think it is. Last summer there were found two centres of in- fection previously overlooked.’ One of these consisting of a croup of six diseased trees, was within a few miles of Washing- ton. In company with Dr. Metcalf and others I had an oppor- tunity to examine these trees on December 30, 1911. One of them, a tree over three feet in diameter, was in an advanced stage of the disease. Large limbs were dead and the lower portion of the trunk was thickly covered with spore masses of the fungus. How long these trees had been affected it was impossible to de- termine, but it is safe to say that some of them had been diseased for at least a year and probably longer. That is to say, they be- came infected in 1910 or earlier and must have been discharging millions of spores at the very moment Dr. Metcalf was writing his statement that the country within a radius of 35 miles of Washington was apparently free from the disease.t It is quite probable that other overlooked cases of the disease exist in the vicinity of Washington at the present time. Further, We visited two places where diseased trees had been removed and the disease “eliminated” in 1909. In one case, one tree had been cut; in the other case two trees. The bark had not been removed from the stumps. On one stump we found a few spore masses of the fungus; also on the base of a nearby tree. On the other two stumps no fungus was found. The first-men- tioned stump had not sprouted, but the other two were -sur- rounded by healthy sprouts. At both points there were a few chestnut trees in the immediate vicinity, but, so far as could be determined, none of them were diseased. It should be stated, however, that it is very difficult to locate diseased trees in win- ter. It is inevitable that the bark around the base of a diseased tree and also the surrounding soil, fallen leaves and other litter will become covered with spores carried down by rain. Hence, when the diseased trees were removed thousands of spores were left behind. How long such spores live and retain their power of infection is not known. Now does it seem probable that the ’ 43 failure of the disease to spread to nearby trees was due to the removal of the diseased trees? Is it not more likely that its spread was prevented by the conditions being unfavorable for in- fection? Returning now to the main question: No such method of controlling a fungous disease has ever been attempted. Our knowledge of fungous diseases in general indicates that it is im- practicable. It will be extremely difficult to locate all of the diseased trees and absolutely impossible to remove all of the fungus after the diseased trees are found. The fungus spores, which are produced quickly and in enormous numbers may be widely disseminated in several different ways, some of which cannot be prevented. The work will be exceedingly expensive and must be continued indefinitely. Taking all these things into consideration, the chances of success are much too small to warrant the expense. It is true that some fungous diseases, notably the plum black knot, are more or less successfully controlled by the prompt re- moval of diseased plants or parts of plants; but it should be noted that the diseases successfully controlled in this way have two characteristics which make this method of control possible: (1) The diseased plants may be readily detected in the early stages of the disease; (2) the causal fungus requires a long time to ripen its spores. Plum black knot may be readily detected from one to several months before the ripening of the spores of the causal fungus. Hence, the knots may be remoyed before they have had a chance to spread the infection. Not so with the chestnut disease. It possesses neither of these characteristics. It is difficult to detect in the early stages, and multitudes of spores may be produced within a month after infection. Undoubtedly, the spores are carried long distances by birds, especially woodpeckers, which visit the diseased trees, seeking borers, in the tunnels of which most of the infections occur.’ It naturally follows that the “Immune zone” must be many miles wide,—Dr. Metcalf suggests ten or twenty miles wide. In this connection, please note that while the main line of infection is now somewhere north of the Potomac river, advance infections already occur in southern Virginia and West Virginia, 150 miles or more southwest of Washington. In fact, Metcalf and Collins 44 say :° “Observations made by the junior writer indicate that the disease may have been present in an orchard in Bedford county, Va., as early as 1903.” The advance infections are widely scat- tered. Back of the “immune zone” extensive areas must be inspected frequently and thoroughly. Should the ‘immune zone” be lo- cated at or north of the Potomac, the entire States of Virginia and West Virginia must be covered by such inspection. ‘There is no knowing when or where the disease may break out, and when conditions for its spread are favorable, a single diseased (ree overlooked may start an uncontrollable epidemic which will necessitate establishing a new “immune zone” farther south and starting all over. It is quite generally admitted that it will be difficult to locate all of the diseased trees, but there is some difference of opinion as to the importance of this fact. ft may be argued that by the de- struction of 90 or 95 per cent. of the diseased trees the spread of the disease will be reduced to that extent. This is very improb- able. If this disease behaves like fungous diseases in general, its spread depends more upon weather conditions and the sus- ceptibility of the host than upon the number of spores produced. When the conditions for its spread are favorable five per cent. of the spores may be sufficient to nullify any attempt to control the disease. All experience with such methods of treatment goes to show that the work must be done thoroughly, else it is not effective. The history of the chestnut bark disease is unparalleled in the annals of plant pathology. Here we have an unknown fungus, none of the relatives of which are parasites, suddenly becoming widespread and taking high rank as a destructive parasite. This indicates that it may be expected to behave in an erratic manner and be unusually difficult to. control; also, that something unusual has happened either to the host or to the fungus, or per- haps to both, making this epidemic possible. Just what this may be I am unable to say. There is no reason for believing that the fungus is either a recent creation or a recent introduc- tion from abroad. The only rational theory yet advanced re- garding the origin of the epidemie is Dr. Clinton’s winter-and- drought-injury theory,’ but even this seems insufficient in some respects. 4D It has been asked “What then would you have us do? Stand idle while the disease destroys our chestnut forests ~ My answer is this: It tay be well to restrict the transportation of diseased nursery stock, but this is all that it is worth while to attempt at present in the line of combating the disease. /t is bet- ter to attempt nothing than to waste a large amount of public money on a method of control which there is every reason to be- lieve cannot succeed. 1 believe in being honest with the public and admitting frankly that we know of no way to control this disease. I fayor moderate-sized appropriations for investigation of the disease, but none at all to be used in attempts to control it by any method or methods at present known. What will be the future course of the disease can only be con- jectured, but it can be safely predicted that nothing which man can now do will materially alter its course. However, the situa- tion is by no means hopeless. That the disease has already reach- ed its zenith and will now gradually subside is quite possible. There’ have been other epidemics, and other kinds of trees and plants have been threatened with destruction through disease, but such a thing has never actually happened. So far as known, no plant has ever been exterminated by disease. It is unlikely that the chestnut will be exterminated. THE CHAIRMAN: It occurs to the Chair that the situation would suggest discussion at this time, but it would probably be hetter to continve with our programme as it was ably laid out by those who have provided for this Conference, and have the dis- cussion after we have heard the papers. We will, therefore, call for the next paper, entitled “How Further Research may Increase the Efficiency of the Control of the Chestnut Bark Disease,” by T'rofessor W. Howard Rankin, Cornell University, Ithaca, New York: 1. Metealf. H. and Collins, J. F. The control of the chestnut bark disease. U. S. D. A. Farmers’ Bul. 467, 28 O. 1911. Ze Oe. 1eib:) D: IL. 3. Reported by Dr. Metealf at a conference on the chestnut bark disease held in Albany, N. Y., October.19, 1911. 4. U. S. D. A. Farmers’ Bul. 467:11. 5. U. S. D. A. Farmers’ Bul. 467:10. 6. Metealf and Collins. The present status of the chestnut bark disease. U. S. D. A. Bur. Plant Indus. Bul. 141, Part V, p. 46. 30 S. 1909. 7. Clinton, G. P. Report of the Botanist, 1908. Conn. Exp. Sta. Rpt. of 1907-1908: 879-890. July, 1909. HOW FURTHER RESEARCH MAY INCREASE THE EFFI- CIENCY OF THE CONTROL OF THE CHEST- NUT BARK DISEASE. BY PROFESSOR W. HOWARD RANKIN, Cornell University, Ithaca, N. Y. Mr. Chairman, Ladies and Gentlemen: Up to this time investi- eations concerning the chestnut tree canker disease and the causal fungus have not brought forth facts as rapidly as we could wish. It was the opinion of the conference held at Albany, N. Y. last October that we did not have facts enough about the disease and that scientific research was the one thing needed. To emphasize this point we may consider some important phases of the disease which are yet little understood, but the knowledge of which is fundamental to devising efficient control methods. Con- cerning the means of spread of the fungus from one tree to another we have nothing except secondary evidence. Most writ- ers have theorized on the different methods by which the conidia or summer spores might be carried from one tree to another and 2 new infection started. . Reasoning by analogy with what is known of the behavior of many fungi, such agencies as borers, birds, ants and the wind, ete., have been suggested but in no wise proved to be responsible. It seems that the ascospore stage has not been considered by any writer in the dissemination of the fungus, yet this stage follows the conidia very quickly and is the more abundant fruiting stage which is formed in the red or brown pustules on the surface of the cankers. Under moist conditions the ascospores are shot forcibly out in the air where they can be caught up by the wind and carried for a considerable distance. The speaker found the ascospores being shot from mature pus- {ules during every rainy period last summer. These spores ger- minate readily in rain water producing a new mycelium of con- siderable length in fifteen hours. The question at once arises, why could not these ascospores once shot into the air be carried long distances and owing to their abundance cause a large ma- jority of the infection? The time of year at which new infee- 47 tious took place last summer in the Hudson River Valley was evidently about the time when the ascospore stage was just be- coming abundant. It is an important matter then to determine the spore stage and the ageney responsible for the spread of the fungus before we can hope to advise an efficient and effective con- trol. For example, such precautionary measures as the peeling of logs before allowing them to be moved could be limited to the time of year when this was necessary and thus obviate a great cost, Likewise the problem as to how the present epidemical char- acters exhibited by the disease have come about is as far from solution as it was six years ago. The speaker has recently col- lected and examined a fungus indistinguishable from the chest- nut canker disease fungus on dead chestnut bark in several places in Virginia. No case of this fungus attacking living trees was found in the short preliminary examination made near Lynchburg, although several specimens were collected on dead bark of stumps from which trees were cut about two years ago. Also a fungus found in Pennsylvania on white, red and black oak has great similarity to the canker disease fungus. The pos- sibility of having several strains of the same fungus identical as to microscopic characters, some saprophytic and others causing a virulent disease, is at once puzzling. One of two things has evidently happened, either the host plant has, under existing conditions, been altered in its physiological process enough to change its susceptibility to this heretofore saprophytic fungus, or the fungus has developed a parasitic habit independent of any change in the host. Possibly, of course, both factors may have combined to bring about this disease-condition. Prelim- inary investigations carried on by the speaker seem to point to the fact that the susceptibility of the chestnut tree to this fungus depends upon drought conditions; that is a low water content in the tree. This requires confirmation however by further detailed experiment. Weather conditions causing winter injury as sug- gested by Dr. Clinton may quite possibly be of importance also in this connection, and accurate data concerning past weather conditions and experiments to determine the effect of low temper- ature on the chestnut tree in connection with the production of susceptibility is highly important. 48 If the results of Dr. Munch on the cause of susceptibility and immunity of forest trees to disease should prove true in the case of this disease also, we may hope to be able to control the bark disease in shade, lawn, and park trees, by keeping up the water content of the tree. Whether nursery stock serves to introduce the disease into new localities is an important problem to be determined by ob- servation and experiment. The present method of inspection and cutting out would be inefficient if the fungus lives commonly as a saprophyte at the base of the tree on dead bark and can at- tain a parasitic habit with some slight change in weather condi- tions. If, on the other hand, it exists only as a wound parasite, then inspections would be possible and the cutting out method effective. However, with such problems as these undecided, no ohne can pronounce definite judgment upon the efficiency of the cutting out method. Once however, these facts are established, modifications may be made in the present method by which its cifectiveness may be insured at possibly a lower cost than can now be expected. The present method which the Pennsylvania Commission has adopted of eradicating only spots where the fungus is distinetly parasitic, can accomplish a great good in a sanitary way, and once sufficient facts are forthcoming, the method may be altered to suit our knowledge and thus its efficiency assured. THE CHAIRMAN: The next paper, entitled “Recent Notes on the Chestnut Bark Disease,’ will be delivered by Professor H. R. Fulton, Division of Pathology, Pennsylvania State College. RECENT NOTES ON THE CHESTNUT BARK DISEASE. BY PROFESSOR H. R. FULTON, Pennsylvania State College, State College, Pa. The steady and devastating spread of the chestnut bark dis- ease brings us face to face with a grave situation, and raises many questions of great importance. Most of these will centre about the three great questions: Is it possible to check effectively Orchard chestnut tree girdled at base, showing characteristic growth of sprouts. Scene near Westbury, New York.—-Photograph by Perley Spaulding. ee " a5 = 7 = * - ‘ ae = , _ ; 7 fe i ! 7 —_ * F " i _ = _ Large forest tree girdled at base, showing characteristic growth of sprouts; near Richmond Hill, New York.—Photograph by Prof. Collins. Large trees with some branches girdled. Note condition of the foliage. Seene at Westbury, New York.—Photograph by Prof. Collins. Large trees with some branches girdled. Note condition of foliage. Scene at Westbury, New York.—Photograph by Prof. Collins. = om Ps a Wah 2 - +z “y Bal Sh he ay . oe 7 , oo _ ts) —_ e, oe. * et ; es wd . _ = | b - = s LJ a - 7 i al se 7 % : ‘> . > A Orchard chestnuts, (grafted varieties), nearly dead. Note sprouts on the trunks. Photograph by Prof. Collins. Orchard chestnut with limb girdled by twig-girdling borer. Easily mistaken at a short distance for chestnut bark disease-—Photograph by Prof. Collins. Examples of tree surgery, showing healing process after cutting out cankers, in treatment of orchard trees. This treatment undoubtedly prolongs the life of the trees.—lhotograph by Vrof. Collins. Example of tree surgery, showing healing process after cutting out cankers in treatment of orchard trees. Will prolong life of tree—Photograph by Prof. Collins. Chestnut tree showing early stage of disease; note small girdled twig on upper part of the tree in the centre of the picture. Large chestnut tree partly dead. Note sprouts with leaves near the top, the dwarfed leaves on the middle right-hand limb, and the healthy lower branches with normal leaves. Scene at Rawlinsville, Penna.—Photograph by Prof. Collins. Bye #1) >, f *\ Rare oe Karly stage of infection in an orchard tree; note girdled twigs with withered leaves at top. Scene in Lancaster county, Penna.—Photograph by Prof, Collins. Complete destruction of the chestnut trees in mixed stand. Note healthy con- dition of trees of other species. Views along Long Island Railroad, near Richmond Hill, New York.—Photograph by Prof. Collins. Complete destruction of chestnut trees in mixed stands. Note healthy condition of trees of other species. Views along Long Island Railroad, near Richmond Hill, New York.—Photograph by Prof. Collins. Small orchard chestnut nearly dead.—Photograph by Prof. Collins. A dying tree on Long Island, New York. Examples of tree surgery, showing healing process after cutting out cankers, in treatment of orchard trees. ‘This treatment undoubtedly prolongs the life of the trees.—Photograph by Prof. Collins. AY the spread of this disease? Is it worth while doing so? > What are the best methods to use = While no one, perhaps, will ven- {ure to prophesy the outcome, all doubtless agree that the great interests at stake justify an aggressive fight; and all alike are anxious to see the warfare waged in the most effective way. Other contests against fungous foes have been won in spite of apparently insuperable obstacles, and we now look back from the vantage ground of knowledge gained through the contests, and wonder that the tasks should have seemed hard. Each year Witnesses the conquest of more than one important pest, just as each year is apt to bring into the limelight some hitherto unob- trusive pest. Mention might be made of scores of animal and plant pests that, in the wide interchanges incident to modern civilization, have been brought into contact with new host species, or with new environmental conditions, and have forthwith en- tered upon a period of riotous devastation. At the present time, federal and state resources are being drawn upon, and concerted state action is being had, in the fights against the gypsy and brown-tail moths in New England, and against the cotton boll weevil in the southwestern portion of the cotton belt. I cannot refrain from recalling to mind the eradication of the cattle tick in certain districts within its range, and the stamping out of yel- low fever in territory under United State jurisdiction, as notable examples of success that has in recent times come from complete knowledge of the situations, combined with efficient administra- tion. As a citizen of Pennsylvania, I take pride in pointing to the successful suppression of the fcot and mouth disease of cattle, during 1908, by the State Livestock Sanitary Board in co-opera- tion with the Federal Bureau of Animal Industry. These were campaigns of quarantine and sanitation. These examples of yery diverse nature do not prove anything in regard to the chestnut bark disease; but they do serve to em- phasize the fact that persistent effort in the right direction may win in the face of great odds. To the specialist in plant diseases, a most interesting question is, Why is it that this disease has made such headway in this coun- try in so short atime. Is it that there are factors involved, aside from administrative difficulties, that are not found in the many 4 50 fungous diseases that affect our crops,—less spectacular in their working, but none the less damaging in their effects? Or is it that well recognized factors are here found in a unique combina- tion that adds to the seriousness of the situation? Is this dis- ease inherently more serious than pear blight or cotton wilt or wheat stem rust? Answers to such questions involve considera- tion of the habits and value of the host plant, as well as definite knowledge on all important points in the life history of the causa- tive organisin, Diaporthe parasitica. lor chestnut bark disease infection to occur, three general con- ditions must be met just as for any other fungous disease. Broadly stated, these are (1) the presence of infective material, (2) a host plant in a condition of susceptibility, (8) general en- vironmental conditions that are favorable. All rational control measures for the disease must be based on the peculiarities of this fungus with reference to these three things. The infective material for Diaporthe parasitica seems to be pre-eminently the spores, which are of two types, the pycnospores, sometimes called conidia or summer spores, and the ascospores, or winter spores. We wish to know definitely the conditions that influence the formation of each type, the longevity of each under favorable and under unfavorable conditions, their modes of shed- ding and of transfer, the conditions favorable and unfavorable to their germination, their abilities to establish the fungus upon various materials, and the relative importance of the two types in spreading the disease. General environmental conditions may have their effect upon longevity of spores, upon germination of spores, upon rapidity of growth of the fungus, and upon spore production by the fungus. Susceptibility in the host has refer- ence to qualities of genera or species or varieties or strains or individuals, that render them liable to attack by the fungus, which qualities may be inherent or possibly induced by environ- mental conditions. Here must be included the exposure through various wounds of susceptible portions of the host; and the pro- tective effects of measures that may lessen the susceptibility of the host. Other points in the general life history of the organism may be of interest and importance, aside from any direct rela- tion to the setting up of infection. 51 Realizing the importance to the public welfare of more com- plete knowledge along these lines, the Pennsylvania Agricultural Experiment Station, through its laboratory of plant pathology, has undertaken certain investigations upon the life history of Diaporthe parasitica, in hearty co-operation with the work of the Pennsylvania Chestnut Tree Blight Commission. While a com- plete report cannot be made, in the nature of the case, for a long time, we beg to submit a brief preliminary report on the labora- tory work now being carried on by Mr. R. A. Waldron, of the Experiment Station staff; to which is added at the request of the xecutive Officer of the Pennsylvania Commission, a summary of field studies made by Mr. R. C. Walton, one of the field agents of the Commission. Credit for the findings reported here is due to the careful work of these two men. AIR CURRENTS AS CARRIERS OF THE CONIDIA. The tests were made with the blast from an electric fan, with a velocity of perhaps twenty miles an hour. The material used was bark of chestnut with tendrils of conidia projecting from the mouths of the fruit-bodies. The tests were made with these tendrils dry, with them moist, and with the spray from an atomi- zer playing over them, the last to imitate conditions prevailing during storms. The attempt was made to catch the spores on the surface of sterilized potato agar exposed about six inches away, in the blast; and to determine the carrying power of the air cur- rent from the subsequent growth of Diaporthe parasitica in this material. Also, wet cotton was similarly held in the blast; it was then squeezed out in sterile water; this was centrifuged, and microscopic examination made of the sediment, as well as cul- tures from it. There was unmistakable evidence, from each line of testing, that the conidia may be detached by strong air currents, and carried short distances. The detachment was greater when the spray played over the material. The test will have to be carried further before quantitative results can be given. It seems likely that the detachment was largely of small bits of the tendrils made up of large numbers of spores, and that these are too heavy to be carried great distances; and suggests that under natural conditions infection may be spread short distances by wind. 52 LONGEVITY OF CONIDIA AND ASCOSPORES. The length of time that conidia retain their power to germinate will doubtless vary with the conditions under which the spores are kept. Spores from bark collected in late summer and kept dry at ordinary room temperature, germinated readily for four months, but three weeks later could not be induced to germinate, Material exposed out of doors and that kept moist and at about 75 degrees T°, in a greenhouse, did not give germination of conidia after four months earlier tests not having been made. GERMINATION OF CONIDIA AND ASCOSPORES IN DIFFERENT MEDIA. Both kinds of spores germinate in a decoction of chestnut bark, in rice broth, ete. Ascospores germinate in spring water, the conidia do not. EFFECT OF TEMPERATURE ON GERMINATION, Conidia germinate best at a temperature of 60 degrees F., and distinetiy less rapidly at temperatures 10 degrees above or below this point. Ascospores germinate best at a temperature of about 70 degrees I*., but a good percentage of germination occurs at 85 degrees F, and 45 degrees I’. Even at 38 degrees F. the germination of as- cospores was 25 per cent. in the first 24 hours, and reached 70 per cent. in three days. Ascospores germinate readily after at least moderate freezing. These facts indicate that the ascos- pores may play a more important part in causing infection under certain conditions, than has been commonly attributed to them. The effect of extremely high and low temperatures on spores has not yet been completely investigated in our laboratory. EEFEECT OF TEMPERATURE ON BARLY GROWTH. In general the most rapid early growth is at the optimum tem- perature for germination. In a nutrient solution of boiled chest- nut bark, the ascospores will send out a length of mycelium 10 to 15 times the spore length in the first 24 hours at 70 degrees F., which becomes an indefinitely large mass of mycelium in two days. At 358 degrees F., the growth is about one spore length the first day, and 15 times this in five days. GROWTH ON OTHER MATERIALS THAN CHESTNUT. In the laboratory the fungus grows well on a variety of artifi- cial media, perhaps most readily on potato agar that has been made slightly acid. Material was submitted to us of white oak and black oak bark, collected by Mr. J. Kh. Guyer, agent of the Pennsylvania Commission, which bark had been killed by fire pre- vious to its observation, and siowe. pustules of what seemed to be Diaporthe parasitica, Careful microscopic examination show- ed that the morphological features corresponded closely to those of Diaporthe parasitica, as did also the growth of the fungous in artificial culture. Red oak twigs killed by steaming in the process of sterilization, were readily infected by Diaporthe parasitica ob- tained from a typical chestnut lesion. While it is desirable to carry on further cross inoculation experiments, it seems rea- sonable to suppose, in the light of present evidence, that Diapor- the parasitica may, under unusual circumstances, establish itself saprophytically on portions of trees outside the genus Castaneda, if these portions are already dead. We have found no evidence that the fungus produces in any sense a disease of such trees as the oak. - RELATION TO LIGHTNING INJURY. In August, 1908, Mr. George Wirt, of the Pennsylvania For- estry Department, directed the attention of the speaker to a chestnut tree in an advanced stage of infection, that had been struck by lightning earlier in the season, when its leaves were half grown. Where the wood had been splintered along the lightning track, there were numerous pycnidia standing apart one from the other, as is characteristic of Diaporthe parasitica when fruiting on wood rather than on bark. Many of these fruit- bodies were deep in the cracks made by the lightning, and evi- dently had been formed after the stroke. Specimens taken from the wood and from the bark near by, when tested, gave good germination of spores. Probably the bark infection, whieh seemed to date far back, existed at the time of the stroke, and the fungus spread from this to the shattered wood, the lightning presumably not having killed the fungus in the vicinity. 54 DEVELOPMENT IN SAPWOOD AND HEARTWOOD. Where a section of a large infected branch was kept in a moist atmosphere constantly, an abundant development of pycnidial fruit bodies was noted in about two months from both sapwood and heartwood at the more moist cut surface. The similar de- velopment in wood shattered by lightning has been mentioned above. In two cases, the fungus was found on young, unligni- fied shoots; in both cases, the parts had been distinctly injured by insects. SUMMARY OF FIELD STUDIES AT ORBISONIA, PA. During the fall and early winter of 1911-12, Mr. R. C. Walton made a detailed study of an advance spot of infection at Orbi- sonia, Huntingdon county, in Central Pennsylvania. The tract covered some forty-six acres on the north and northwest slope of a mountain. It had been cut over originally forty-five years ago, and at intervals since, the last cutting being in 1908. Most of the chestnut growth was coppice of four years standing. Rather severe fire injury had occurred in 1902, and the land had been pastured recently. Soil conditions and density of stand varied considerably over the tract. The infection was found in detached spots over about thirteen acres. There was one spot that seemed to be the original centre of infection, dating back two years; but elsewhere in the area there were lesions apparently as old. Altogether three thousand and fifty-nine chestnut trees, - sprouts, and stumps were examined and tyo hundred and eighty, or 9.1 per cent. were found to be infected. Of these, practically all were four year coppice growth. The oldest lesions were seemingly two years old, and ten of these were found. The youngest were for the current season, and of the total, about half seemed to be less than one year old; and estimates of the age of all the leSions indicated a very uniform rate of spread during the two years. It may be added from a recent investigation that 153 trees in southeastern Pennsylvania, near Haverford exposed to natural infection, carefully examined and marked as unin- fected in January 1911, showed 25 trees infected in a recent ex- amination. This would indicate something, perhaps, of the ‘apidity of the spread ot the disease, where observations were made upon that point. ~ AD Out of 18 sprouts showing two lesions, 15 had the younger lesion above and 5 the older, which might indicate the probable work of insects in carrying infection. Sprouts were originally infected at the base in more than four- fifths of the cases. Forty per cent. of the oldest lesions on sprouts showed twigs as a centre of infection; eighteen per cent. showed cracks, fourteen per cent. wounds; thirteen per cent. beetle holes, eleven per cent. crotches, and four per cent. were in- determinate. More infections were found in medium dense growth than in dense growth, and yery few in rather open growth. Of all in- fections recorded, 47.3 per cent. were within twenty feet of old logging roads, 7.4 per cent. from 20 to 50 feet away, and 45.5 per cent. at greater distance. Many more infections were found where soil conditions were moderately moist than where they were dry. Of 150 original sprout infections, 62, or 41 per cent. had a north to northeast exposure; 20 or 15 per cent. a south to southwest exposure; and the remainder ‘were about equally divided between the other two quadrants of the compass. This might suggest moisture again as an important factor. There were 28 cases of pycnidia observed developing on wood. Only eight trees larger than seven inches in diameter showed in- fection. One of these had a lesion apparently two years old; and half had the oldest lesion less than one year old. ] ‘Can you?’ said the credulous woman, looking up to the dead top of a noble tree. ‘I have noticed that there was something the matter with them. Tow much will it cost?’ ‘Let’s see,’ mused the tree-doctor. ‘Eleven trees, two dollars apiece. Well, I'll make it twenty dollars for the lot. They're worth more than that to you, ain’t they?’ 60 ‘I should say they were,’ said the owner of the estate. ‘My husband said before he died that he wouldnt take five hundred dollars for that big chestnut out in front there. I will willing!y pay twenty dollars to have them saved.’ ‘All right. Let me get my outfit.’ He went to his buggy, brought back a paper bag of powder and a whitewash brush, and borrowed a pail, some water and a step- ladder. In an hour he had swabbed the trees from as high as he could reach from the ladder down to the ground, pocketed the pleased widow's twenty dollars, got into the buggy, said ‘Gid- dap’ to his horse, and was down at the next door yard, swabbing more trees and pocketing more dollars.” It is true that many unscrupulous persons have been making money in a manner similar to the one mentioned in this story. It is true also that the ravages of the disease, and especially the legislative appropriation to combat it in Pennsylvania, have sud- denly brought to light numerous unsuspected infallible cures for all the ills (including the chestnut bark disease) to which trees are or ever will become heir, if we should judge only from the statements of the advertisers and inventors. Apropos of this, the Chestnut Tree Blight Commission of Pennsylvania might relate some of their experiences along this line that would make more interesting reading than the above, though the incidents were less profitable financially to the fakirs. The main point that I want to emphasize, however, is that the value of ornanmental trees cannot, like forest trees, be gauged by the mere timber value of the wood, nor, like the orchard tree, merely by the value of the annual crop of nuts. The chestnut tree undoubtedly attains its highest value as an ornamental tree. You will all recall, I am sure, certain estates where one or more chestnut trees are the main aesthetic or decorative features. Per- haps the tree may have been a veteran, famous in the country- side, long before the present owner purchased the land and built his domicile. Oftentimes the value of the ornamental tree is largely enhanced by its location with reference to the house, and even more largely, at times, by historic or ancestral traditions with which if may have been, lone since, associated. The value placed by the owner of the estate upon such tree may oceasion- ally be almost without limit. 61 The very fact that the tree is of much greater value to its owner than any tree in the forest could be, means that more labor and more care, can and will be expended upon it, if it needs it, than would be considered possible, from almost any economic point of view, on either the orchard or the woodland tree. Con- sequently some methods of combating the disease may be profit- ably applied to ornamental trees that would not for a moment be cousidered in connection with a tree in the forest. At the very beginning of the experimental work undertaken by the United States Departinent of Agriculture, this fact was recognized, and has since been kept in mind. Considerable of the experimental work has had for its main object the solving of the problem as to whether or not it will be possible to eradicate or control the disease on individual trees. Notwithstanding the fact that much of this work has been done in chestnut orchards, there are probably few orchard trees that would be worth the expense involved in an attempt to save them; however, on account of their smaller size and greater ac- cessibility, they would be more profitable for individual treat- ment than the forest tree. Consequently these orchard trees be- come, in most cases, nothing more or less than experimental martyrs for the possible future benefit of their more aestheti- cally valuable ornamental kin. It is yet much too early to make a very definite statement, cer- tainly not a final report, upon the possibilities of being able to control fully the Chestnut Bark Disease on ornamental trees without recourse to the radical methods at present advocated for controlling it in a woodland. Nevertheless, certain facts have been repeatedly demonstrated in the course of the experi- mental work which apparently point in a very encouraging man- ner to the probable ultimate accomplishment of this highly de- sirable end though perhaps not on a very encouraging economic basis, as such a basis is usually figured. IT want to call your attention to some of these facts, as well as to the bearing that they may have upon control work of this general character. But in order to make clear certain points I must first refer very briefly to the general line of treatment which is being followed in the experimental work mentioned. 62 This has been fully described in Farmer’s Bulletin No. 467, of the United States Department of Agriculture, and need not be considered in its entirety here. For this work the most essential implements are a gouge, a mallet or hammer, a pot of tar or paint, and a brush to apply the latter; also a whetstone for keeping the gouge sharp. When a diseased spot in the bark is located, it is carefully cut out with the gouge and mallet, care being taken to cut the bark perhaps one-half inch beyond the discolored area which is usually so prominent a characteristic of diseased bark. It is extremely im- portant that the gouge be kept scrupulously sharp. If it is dull, the pressure required in forcing it through the bark will invari- ably result in some injury to the delicate cambium cells at the edge of the cut. This means that the new growth will start back under the bark some distance, an eighth, a quarter, a half inch, or even more, and not close to the edge of the cut, where it should start under the most favorable conditions. During the growing season the new growth begins to lift the old bark within a week or ten days. If this growth does not be- vin close to the edge of the cut, we shall find in the course of three weeks, under the uplifted edge of the bark, the finest kind of a shelter for all kinds of small grubs, beetles, ete.; all of which are well known danger factors in connection with the spread of the disease. At most seasons of the year, it is highly important that the edge of the cut along the cambium line be covered with paint or tar as promptly as possible. This is an important, and often essential, point in coaxing the new growth to start closer to the edge of the cut than it ever would under perfectly normal conditions. By using a sharp gouge and promptly covering the cut edges, we have many times had the satisfaction of seeing the new growth start within a thirty-second of an inch of the edge of the cut, and be readily visible to the unaided eye in less than a week. Anything better than this can scarcely be expected. Of course, all portions of the cuts must be finally, carefully and completely painted with tar, paint, or other suitable waterproof coating, and it is, theoretically at least, a good plan to paint the cut surface with copper sulphate or Bordeaux before waterproof coating is applied. 63 In discussing ‘the possibilities pro and con of controlling the disease on individual trees after it has become established, there are many factors that should be clearly understood and carefully considered. It should be determined just what bearing each will have on the main problem, just how each unfavorable one can be overcome or at least neutralized, just how each favorable one can be made even more helpful in the fight; all these, and more, if we are to enter the combat fully equipped. Irom numerous points of view it is extremely unfortunate that the disease has spread with such rapidity from its first known centre, that nearly every person who has been detailed by the States or the federal Government to work on the disease has, of necessity, been obliged to devote most of his energies to lo- cating or destroying infected trees, and relatively little or none to the research or investigation phase of the problem. Everybody who has had much to do with the disease will agree with me, I am sure, when I say that in our efforts to con- trol it we have been enormously handicapped by lack of just such knowledge as comes only from systematic and painstaking research. If we had this knowledge at the present time we would undoubtedly see with clearness many things which are now shrouded in the mistiness of uncertainty or in the darkness of complete ignorance. Who, I wonder would venture to foretell the effects upon the whole question of control if we had spread before us a complete, or fairly complete, positive knowledge of the many important points connected with the disease, about which we now know so little; e. g., to mention a few of these, its origin, methods of dissemination, detailed effects upon the host, immediate cause of the death or the lost vitality of the spores, resistance of spores and mycelium to toxic agents, climatic in- fluence upon host and disease, the extent to which it is possible artificially to introduce various fluids into the circulatory sys- tem of a tree without killing it, the extent to which insects are responsible for the spread of the spores, the precise knowlege of the relation of birds, rodents, wind, etc., to dissemination of the spores. In attempting to control the disease on individual trees, there are certain facts, as I have already stated, which have been re- _* 64 peatedly demonstrated in the course of experimental work, that are worthy of consideration at this time. I want to mention and very briefly discuss six of these: (1). Lateral or oblique conduction. There seems to be a rather widespread (but erroneous) idea that the crude aud elaborated sap of a tree can pass up and down the trunk or branch only in a longitudinal direction, that is, lengthwise of the fibres or “grain” of wood or bark, or at most with but slight deviation from this route. The fact that it is transferred almost entirely in a longitudinal direction in a healthy uninjured tree may be true enough under normal con- ditions, but it is far from true in trees that have been injured in certain ways, and, as all students of plant physiology know, not strictly true under perfectly normal conditions. It is a fact of common knowledge that a tree will ordinarily cover or grow over, an area of bare wood where the bark has been removed. It is common knowledge to all observant persons that these scars heal over mainly from the sides. In all proba- bility this is largely because they adjoin the uninjured vessels through which sap is being conducted in the normal longitudinal direction, but doubtless in part also to other causes to which I shall allude directly. If a partially or entirely healed over scar should be dissected, it will be found that in the layers of wood formed immediately after the injury the fibres are curved out- ward around the injury, and continue in a nearly longitudinal direction both above and below the scar. When the scar is par- tially covered, the newly formed fibres are straighter, and finally after the scar is entirely covered, the youngest fibres will be found to have assumed their normal longitudinal direction, or very nearly so. If it were not for this possibility of oblique conduction, a tree that had a large lesion extending half way around the trunk on the north side, for instance, and an equally large one on the south side, either above or below the other, would, to all intents and purposes, be girdled. In the chestnut tree, the angle from the perpendicular to which these fibres can be made to curve, as a result of experimen- tal cuttings, may seem surprisingly great. In one instance the O65 writer very nearly succeeded in an attempt to force this new growth to produce fibres at right angles to the normal direction : i. e., they were made to bend more than SO degrees. The fact that new fibres can, if necessary, be formed at such a great angle from the normal is of very great advantage to the chestnut in the process of healing over scars made, for example, by cutting out diseased spots in the bark. As food is conveyed through a plant in very dilute watery solutions, it is necessary that a great amount of sap be circulated or conveyed to a point where any considerable’ amount of food is demanded. If the tubes which primarily convey sap should be severed, as when a diseased spot has been cut out of the bark, the free transfer of Sap is at most seasons of the vear immediately reduced to a mini- mum in the severed or “dead ends” of these sap conducting tubes, which from the point of view of circulation, now hold about the same relation to the uninjured tubes that the stagnant arm of a river does to the main river. So far as the actual food is concerned, it is obvious that the amount of sap necessary to supply the requisite food cannot reach the upper and lower edges of a scar by means of the dead ends of the conducting tubes as readily and rapidly as at the edges where there is a. continuous stream of sap passing along the uninjured tubes. Oftentimes just below a broad scar which reaches to the wood, and less often above it, a triangular piece of bark will die. This is due directly or indirectly to the inability or great difficulty that the sap has in reaching these places. In order to preclude the possibility of the bark dying back either above or below a sear, and thus furnishing favorable shelters for insects, the top and bottom of the scar should be pointed instead of allowed to remain abrupt or rounded. Under ordinary conditions it takes no longer for a scar six inches long and an inch wide to heal over completely than it does for one an inch long and an inch wide, simply because the healing over depends almost entirely upon the growth at the sides of the scar. As I have already in- timated, all cuts should be made with instruments that are kept very sharp. (2). Mycelium in the wood. 5 66 The mycelium of the fungus almost always produces a very characteristic mottled fan-like appearance in the bark, and ap- pears to penetrate through the tissues of the bark but a short distance, if at all, beyond this discolored area. The mycelium also penetrates the sapwood very freely, when the disease reaches as deep as the wood, as it generally does sooner or later; but, unlike its effect in the bark, no pronounced discoloration is pro- duced in the wood, and it is impossible to determine with the unaided eye the approximate limits of the mycelium, as in the case of the bark. In all efforts to control the disease without destroying the tree, it is of course necessary to gouge out this disease infected sapwood. The depth to which it is necessary to remove it can- not at present be definitely stated, as insufficient time has elapsed to demonstrate this point experimentally. Many cut- tings, some with the sapwood partially removed from beneath a lesion, and others with all of it removed, are now being watched for results. However, in a diseased spot from three to four inches in diameter apparently at least three annual layers of wood in the centre of the diseased spot must be removed. Of course where sapwood is cut, enormous numbers of minute tubes, which conduct the crude sap from the roots through the trunk and branches to the leaves, are severed, and, should the cutting happen to have been done during warm, dry weather, it often happens that one or more branches directly above the cut-out area will show much wilted leaves within an hour or two. This is a direct and inevitable result of the suppression, from any cause whatsoever, of a considerable portion of the sup- ply of water for the leaves. Considerable careful judgment may at times have to be used when making cuts of this nature, and occasionally it may be wise to remove one or more healthy limbs, or perhaps to strip the foliage partially from a branch situated just above a place where much sapwood has been removed. This will at least tend to pre- vent wilting, which if excessive, may result in the subsequent death of the branch. (8). Preservation of exposed wood from decay. If exposed surfaces of wood are left with no protective cover- ing they soon become weathered, dried, checked, and easily in- fected with fungi, causing decay of the wood, In the chestnut, wye 67 moreover, there is the additional danger of infection from the spores of Diaporthe parasitica. In order to reduce the chances of infection from wood rotting and other fungi, it has been the prevailing custom for many years m this country as well as abroad, to paint all exposed surfaces of wood with tar or lead paint. Judging from our own experience perhaps these are as good general preparations for this purpose as any that we care to recommend at this time, though they are not ideal and they do not prevent the checking of the wood. Moreyer, they must be renewed from time to time in order to accomplish permanent good. Creosote is excellent for a preliminary coating, but it sinks into the wood readily and apparently has waterproof quali- ties of only temporary value. It should always be followed (within a few days, for example) with some thick or heavy coat- ing, such as_ tar or paint. For preventing the drying back of the cambium layer at the edge of a cut, we have so far found nothing better than orange shellac. This does not long remain a waterproof covering under ordinary conditions, and should, as in the case of creosote, be covered with a heavy coating of paint or tar, say within two or three weeks after it is applied. Many other preparations for covering exposed wood have been tried, but those mentioned ap- pear to have been the most satisfactory from the point of view of our experiments on ornamental and orchard chestnut trees. (4). Sanitation. In cutting out diseased spots in the trunk or branches of chestnut trees, the chips should be carefully gathered in papers, or better, paper bags, and destroyed by burning. They should not be left scattered about on the ground. In other words, sani- tation is one of the essentials for success in this kind of work, just as it is in the case of diseases of human beings. In all of our experiments with the disease on one particular plot the chips were left where they fell. No attempt was made to de- stroy them. Later many of these chips were examined and ap- parently good, though dormant, fruiting pustules were present in the majority of cases. To take one particular case: In March, 1911, some diseased spots, with good fruiting pustules, were cut from a chestnut tree and the chips left on the ground in a sunny exposed place on a dry hill-top. These remained on the ground ; 68 throughout the spring, through the hot dry weather of early July, and the drought of July and August. In early September, two days after the almost unbroken week of rain during the lat- ter part of August, these chips were again examined, and on a few of them which were composed entirely of bark, two or three inches long and half as wide, many spore threads were found. These, remember, from chips that had been lying on the ground for more than five months through the hot summer drought. Pos- sibly this may be regarded as an extreme case, but in any event it clearly emphasized the necessity of extreme care in destroying -all diseased bark, chips, ete., in all attempts to control the dis- ease. Again, extreme cases of the sort mentioned are often the very ones that must be guarded against. In certain instances a gasoline torch has proved an efficient adjunct for the burning out of the diseased spot and thus destroying the fungus, whether or not followed by the gouge and mallet. (5))5. insects: Soon after beginning work on the disease in 1908, our atten- tion was irresistibly drawn to the evident intimate relation that insects bore to the spread of the disease. It is singularly inter- esting to note that practically every person who has been work- ing on the disease in the field for any length of time has, sooner or later, been strongly impressed with this very apparent inter- relationship between insects and the chestnut bark disease. Per- sonally, we have made many observations upon the topic, but as this work properly belongs to another Bureau of the U. 8. Dept. of Agriculture, we have limited our work to observations. flere is a phase of the work that could easily influence the plans of control to a large extent if we knew absolutely the relation of insects to the disease. It is gratifying to know that the Commis- sion has an expert entomologist already at work on this particu- lar part of the general problem. (6). Immunity. From what is now known regarding the spread and virulence of the Chestnut Bark Disease, there seems little immediate promise of individual trees or variations of the American Sweet Chestnut (Castanea dentata) developing immunity. As _ this + species is the only forest tree of the genus in the country, 1 69 would appear that the question of immunity can have practically no direct or immediate bearing upon the saving of our forest chestnut trees. At the present time there is every prospect that we can rea- sonably expect to procure immune pure bred varieties or species of chestnuts from northern Asia and Japan. Indeed, we already know that some of the Japanese and korean chestnuts are al- most, if not quite, immune to the disease. I think it is safe to say, Where Japanese varieties have been killed by this disease, that in more than ninety per cent. of the cases which have come under our personal observation, the trees have been grafted with Japanese scions on American or Huropean stocks, and the Jap- anese trees have been killed by girdling below the graft. We have repeatedly observed such cases where the stock has been absolutely covered with disease up to the graft line, with not a sign of it anywhere on the Japanese portion. Naturally, this fact in itself is strong proof of the immune nature of these particular Japanese varieties. As these highly resistant, or perhaps im- mune, trees are with us small, and the nuts, though often huge, are of inferior quality, their value will be almost entirely as or- hamental trees, and probably never, in our time at least, of any value in replacing the American chestnut. If the better flavored native and Paragon nuts should disappear from the market, we would doubtless soon turn to the inferior Japanese nut as a sub- stitute. In recent years much has been accomplished along the line of breeding hybrids or strains of plants which are not only often fine in quality, but also highly resistant to disease. The results that have been attained in this direction within a comparatively few years are truly gratifying, but the future will witness greater results. There is no reason to doubt that we may eyen- tually see an immune hybrid chesinut that will rival the Ameri- can sweet chestnut in flavor of the nut, and the Paragon in size. THE CHAIRMAN: You will recall that, while we were listening to the addresses in response to the remarks of Gover- nor Tener, the gentleman from Connecticut stated that he had 70 some results which he desired to present to us at sometime dur- ing the Conference. It has been suggested to me that, as it is a little late, it would be best to put over all general discussion until this evening, when we are to have only one set paper and at this time to call upon the gentleman from Connecticut, Pro- fessor Clinton, who has his results in the form of two short papers. If that meets with your approval, then, we will ask Professor Clinton to speak at this time. He is not “a long, lean man with a grizzled beard,” but he has some other points that will commend themselves to us. (Applause). PROFESSOR GEORGE P. CLINTON (Botanist, Connecti- cut Agricultural Station): Mr. Chairman, Ladies and Gentle- men: The first paper that I will present is written by Profes- sor Farlow, of Harvard University. For the benefit of those who do not know Professor Farlow, I will say that he is the oldest mycologist in this country, has had the greatest experience in studying fungi and has some of the best herbaria dealing with fungi, especially those bound in book form, known as Exsiccati, in the world. He took up the study of the nomenclature of the chestnut blight disease, at my request, about two years ago. He has not supplied a title to the paper which I will now present. PAPER BY PROFESSOR W. G. FARLOW, HARVARD UNIVERSITY, MASSACHUSETTS. The cause of the disease of chestnut trees prevalent in our Kastern States is ascribed to the growth of the fungus named Diaporthe parasitica by Murrill in 1906. If as is generally be- lieved, this fungus is the cause of the disease, in searching for the best method of combating it we not only should obtain all the information possible in regard to the microscopic structure and pathogenic action of the fungus, but we should see whether we may not get some practical suggestions from what has been written in regard to the distribution and pathological action of fungi which are most nearly related to our chestnut fungus. The first question we may ask is: Is Diaporthe parasitica, as at first supposed, really a species new to science? If so, is it a native species which has hitherto escaped the notice of our my- cologists, or has it been introduced from some other country? In disease due to fungi the presumption is always in favor of the 71 theory that they have been introduced when they produce sudden and virulent epidemics, as in the case of the potato rot. The presumption, I say, is in favor of this theory, but a presumption it should be borne in mind is not a certainty. If Diaporthe para- sitica is not a species new to science, what is it, and where did it come from? The microscopic structure of the chestnut tree fungus as we now know it, is well known, and its habit and its reproductive organs have been described and figured in many publications accessible to everyone. What, however, is not so generally known is what has been written in times past on fungi found on chestnut trees in different countries, and a review of What is known to mycologists in this connection may he instruc- tive although, it must be admitted, the subject is not very easy to follow. On account of dried specimens in the older herbaria and a summary of the often obscure and conficting descriptions to be found in old treatises, even if desperately dull, will enable us to form certain practical conclusions. When I first received fresh specimens of the fruiting fungus of the chestnut tree I was struck by their great resemblance to what is generally known in American herbaria as Hindothia gyrosa. Unfortunately most of the specimens of that species in herbaria are sterile and from the habit alone one cannot be sure of the species of a fungus of this group. The fresh fungus also recalled a specimen I had seen in an Italian collection, and on looking it up and comparing it miscroscopically with the fresh material, I found the two to be identical. The gross structure and the characters of the spores and asci were the same in both. The Italian specimen to which I refer is No. 986, First Series of the Erhario Crittogamico Italiana, issued in 1863. The label states that the fungus grew on chestnut trunks at Locarno on Lake Maggiore, where it was collected by Daldini in 1862. The name there given is Endothia radicalis, but the question of the name need not be considered at present. As other botanists have examined the specimen just mentioned and agree as to the identity of the Endothia radicalis and the Diaporthe parasi- tica, some having already expressed their opinion in print, we may state definitely that our American chestnut tree fungus does not appear to be new but to have been known on chestnuts in Italy fifty years ago. (2 It may be well to glance at what has been written on the sub- ject in Italy. The earliest reference known to me is that of Ru- dolphi in Linnaea, 1829, where the Hndothia is said to grow on Quercus Ilex, Q. pubens and Castanea vesca. Later accounts were given by Cesati and De Notaris in 1863 in their Schema and the Sphaeriacei Italica, where there is a good description and a rather crude figure apparently drawn from somewhat immature specimens, for the spores are represented as one celled, although in the description they are said to be sometimes obscurely two- parted. The fungus is said to be common on dried branches and denuded roots of oaks and chestnuts in Northern Italy and to occur also on elms. Italian specimens were distributed in Rabenhorst’s Herbarium Mycologicum, Thuemenis, Mycotheca Universalis and Saccardo Mycotheca Veneta; but in the copies which I have examined the specimens had spermogonia but no asci. The most recent notice of the fungus in Italy is that of Traverso in Flora Italica Cryp- togama, in 1906, who uses the name Nndothia gyrosa, It is said to grow on Aesculus, Alnus, Carpinus, Castanea, Corylus, Fagus, Juglans, and Quercus, and to occur not only in Europe and North America but even in Ceylon and New Zealand. We have early notices of the fungus in I*'rance. In 1830 Fries stated in Linnaea that he had received it from that country and Tulasne in his Carpologia, Vol. II, 1863, gave a long notice of the fungus, which he says grows on Carpinus, with critical notes on the synonymy of the species. In 1870 Fuckel recorded its appearance as rare on Alnus at Oestrich in Nassau, and Winter, in 1886, in Rabenhorst’s Crytogamen Flora, stated that the Wn- dothia grew on different deciduous trees in Germany. The records of the fungus in France and Germany are less satisfac- tory than its record in Italy, and the specimens distributed from the former countries in exsiccati are few and poor. Irom this rather long account of the history of the chestnut fungus in Kurope, we may draw the following conclusions: Our chestnut tree fungus is widely spread in Europe and is common in Northern Italy, where it was first noticed as long ago as 1829. It is of interest to notice that writers are very generally agreed that it grows on bark, dried branches, and dead roots, rather than on living branches, and the hosts on which it is said to grow are not merely chestnuts and oaks but a considerable number of deciduous trees. Yet, although the fungus has been so well known in Italy, where it is in some places certainly common, there is no record whatever of any serious disease of the chestnut due to it. The chestnut, which is a tree of great economical im- portance in Italy, is subject to a good many diseases which have been carefully studied by the Italian pathologists but, so far as I know, not one has suggested that any is due to the Endothia. Were it a fact that the Endothia, whatever specific name we please to call it, is a species endemic in Italy but not found in North America until the appearance of the present epidemic, we could understand why the fungus might cause a serious disease in this country although it causes no trouble in Italy, for, if in- fected plants were imported from Europe, the fungus, as in other well known cases, might be transferred to our native chestnuts which unlike the chestnuts of Italy have not become immune. Italian botanists did not and do not regard their chestnut En- dothia as merely an endemic species but consider it to be the same as Nphaeria radicalis described by Fries in 1828 from North American specimens collected by Schweinitz. We learn from Schweinitz, in his North American Fungi, that the species Was very rare on roots of Fagus in North Carolina. The syn- onymy is too complicated to be followed here but some reasons why it is so complicated should be stated. Prior to the publica- tion of N. radicalis, Schweinitz had in 1822 described a Sphaeria gyrosa from North Carolina said to grow on Fagus and Juglans. Later Fries made this species the type of a new genus, Endothia. The earlier Italian writers regarded NS. gyrosa and NS, radicalis as two distinct species, apparently basing their opinion on the fact that Fries placed the two in different sections of the old eenus Nphaecria rather than on an examination of American specimens of the two species. Traverso and some later writers, however, consider that the so-called two species are really only two different stages of a single species. It appears to me that their opinion is quite possibly correct, but the question can be settled definitely only by an examination of original Schweinit- “zian specimens. Thanks to the kindness of Dr. Stewartson Brown I have been allowed to examine the specimens in the Schweinitzian Herbarium in the Academy of Natural Sciences 74 in Philadelphia, and I have also examined Schweinitzian speci- mens in the Curtis Herbarium at Harvard. Unfortunately I have not as yet succeeded in finding a Schweinitzian specimen of S. radicalis which shows ascospores; possibly none of the so- called S. radicalis has ascospores, but I am not yet certain that that is the fact. Specimens supposed to be S. gyrosa are com- mon in American herbaria and have frequently been distributed in different sets of exsiccati. Unfortunately of the considerable number of specimens I have examined, the greater part were sterile although judging by the habit alone, they might very well be S. gyrosa.. I have, however, seen no specimens in the older American herbaria where the fungus supposed to be S. gyrosa was certainly growing on chestnut. In general the hosts were not specificially stated but a large per cent. were evidently on oak. There is a fungus common on oak in the Southern states which has the external habit of Endothia, and appears fre- quently in herbaria as HLndothia gyrosa. An examination of a number of fertile specimens on oak from different localities, hay- ing all the appearance of being 1’. gyrosa, has shown that the as- cospores are unlike those of the Endothia of Northern Italy or like those of what is called Diaporthe parasitica. Stated in words the differences may seem to be slight but in practice one can without difficulty distinguish the two. The spores of the form on oak have hardly half the diameter of those of the chest- nut and the spores are nearly linear. Naturally no definite ae- count of the spores was given by Schweinitz and therefore ex- cept by an examination of authentic specimens we are not able to say whether the form on oak should be considered the true NS. gyrosa of Schweinitz or not. As I have said, I have not yet been able to complete my examination of original material, not as yet having found mature S. radicalis. Although further examination is necessary before expressing a final opinion, certain facts seem to be settled. Our form on chestnut called Diaporthe parasitica, described in 1906, and that on chestnut in Italy collected by Daldini in 1862 are identical as far as can be determined by a study of the dried, herbarium specimens which we have been able to examine. As far as I have been able to examine the older herbaria, I have found no speci- men of Endothia on chestnut in North America. There is, how- 75 ever, an Endothia on oak not uncommonly found in fruit in the Southern States which has spores which seem to me to be speci- fically different from those found on the chestnut. The question, however, is still open as to whether the form on chestnuts may not also be found on oaks on further examination. If so, how- ever, it must be less common, if I may judge by the considerable number of specimens [ have examined, than the form with nar- row, linear spores. DR. JOHN MICKLEBOROUGH, of Brooklyn: Mr. Chair- man: I would suggest that Professor Clinton be given the first opportunity to present his own paper the first thing this evening. We have had a very long session, and [ think the time has come for adjournment. THE CHAIRMAN: That seems an excellent ‘Suggestion. What is the pleasure of the Conference? Is there objection to it? If not, then, Professor Clinton, if it is agreeable to you, we will ask you to present the other paper the first thing this even- ing. The Chair will remind you, gentlemen, that you are invited to register and he would state, also, that the Committee on Reso- lutions will be announced to-night. We will then now stand in recess until sharp at eight o’clock, when we will again meet in this chamber. EKVENING SESSION. Tuesday, February 20, 1912, eight o’clock P. M. THE CHAIRMAN: Gentlemen, the meeting will please be in order. We will first hear the short paper that we had expected to hear at the close of the afternoon session, by Professor Clin- ton. (Applause). SOME FACTS:AND THEORIES CONCERNING CHEST- NUT. BEIGET; BY PROFESSOR GEORGE P. CLINTON, BOTANIST, AGRICULTURAL EX- PERIMENT STATION, CONNECTICUT. Mr. Chairman, Ladies and Gentlemen :— At a recent meeting of the American Phytopathological So- 76 ciety held in Washington, D. C., during a discussion of the chest- nut blight problem, the writer made the following predictions: (1). That chestnut blight was not imported into the United States from Japan; not saying that it does not occur in the lat- ter country. (2). That it is a native American species. (3). That it is a previously described species. (4). That there is evident relationship between its rise and spread in this country and weather conditions. (5). That it is impossible to eradicate it by the cutting out method. (6). That there will in time be a decline in its prominence due to natural conditions. (7). Unpublished—by which was meant that the fungus oe- curs in Kurope. I propose here to discuss some of these predictions, thus giv- ing my reasons for presenting them. There have been advocated two almost diametrically opposed views concerning the chestnut blight in this country. The first of these, if I understand it correctly, assumes that the chestnut blight is a recently introduced disease, apparently from Japan, and that its spread and destructiveness here have not been at all influenced by weather conditions; that if left un- controlled, it will continue to spread and devastate our forests until they are practically ruined, The second view, advanced by the writer, assumes that the chestnut blight is a native American fungus, apparently also indigenous to Europe, and that weather and other unfavorable conditions, which have weakened the vitality of the chestnut trees in the northeastern United States, have had much to do with its sudden, destructive, and wide-spread appearance, and that it will not necessarily wipe out all of our chestnuts, as it is likely to decline gradually with the disappearance of the fae- tors that have favored its rise into prominence. Between these two extremes there are those who take one or the other view in modified form, or agree in part with both. It is highly important that the truth of the matter be ascertained, since upon the nature of the fungus and the manner of its appear- 77 ance in this country depend in large measure the pragticability or impracticability of the only method now advocated for its control, namely, the cutting out and destruction of the diseased trees. c Before proceeding to a discussion -of the reasons why L hold the view I do, let us consider for a moment the apparent reasons for the other view. So far as I can make them out, they are as follows: (1). The trouble appeared suddenly and seriously, and as it is unusual for a fungus thus to spring up in a country where it has never been known before, it is presumably an imported one. (2). But such a serious disease of chestnuts has never been known before in any other country. However, insects and weeds and fungi also, that have been comparatively inconspicuous in their native countries, when introduced into a new country, sometimes develop into serious pests because of their new and unusually favorable surroundings. (3). The Japanese species of chestnut has apparently shown considerable immunity to the chestnut disease, more so than any other. It may therefore be supposed that the fungus is an in- conspicuous native of Japan, and was brought into this country on seedlings from there. It spread to our native chestnuts, and finding these much less resistant to its attacks, has suddenly spread through the regions in which it is now known to occur. (4). The preceding statements being true, there is no reason why it should not go on spreading, and annihilating the chest- nuts of the eastern and southern United States. (5). Preliminary cutting out experiments in a region with- ’ in thirty-five miles of Washington, D. C., are claimed to have prevented the spread of the disease in that region, and based on this, the much more extensive work in Pennsylvania is now being carried on, and similar work is advocated in other States to prevent its further spread through the south and west. Now, if the preceding points are true, Pennsylvania has pos- sibly taken a wise step in trying to control the disease. That it ~ ean ever be eradicated, the writer does not believe for one in- stant, and he has serious doubts about the control being effective or financially profitable, since it means a continuous fight, much 78 like the gypsy moth work in Massachusetts, to prevent re-infee- tion. If the above points, however, are not true, it seems to me, at least, that the efforts for control planned for this State will be time, money and trees thrown away. The author of the first view has not, to my knowledge, claimed that the chestnut blight was imported from Europe, or that the Kuropean chestnuts in this country are especially immune to the disease. If he should ever advocate that it is a Kuropean im- portation, I do not see how he can account for the fact that it has caused no very noticeable trouble on that continent, and yet, when introduced here, kills off the European chestnuts as readily as the native ones; unless he admits that weather or other con- ditions have been unfavorable for these chestnuts, and have thus favored the development of the fungus. Proceeding now to my own theory, let me take it up point by point. First, that the chestnut blight is a native of this country. In 1909 I sent to Professor Farlow, of Harvard University, the first specimen of Diaporthe parasitica that he had examined, and asked his opinion as to whether or not it was the same as a cer- tain species that Schweinitz had years before described on chest- nuts from this country. He replied that it was not, but that it agreed more perfectly with the genus Endothia than with Dia- porthe, and that it was closely related to, but apparently dis- tinet from, Lndothia gyrosa. Endothia gyrosa was originally described from Carolina and Pennsylvania by Schweinitz as Sphaecria radicalis and Sphaeria gyrosa, and reported by him on Fagus and Juglans. It has since been reported in the United States on Liquidambar and Quercus species, chiefly on the lat- ter. : With the clue furnished by Professor Farlow, I found and so stated in my 1908 report, that a specimen of Hndothia gyrosa on chestnut collected by Scarrado in Italy had been issued in de Thuemen’s Myc. Univ. No. 769, and that so far as its gross ap- pearance and pyecnidial stage (the only stage present in my speci- men) were concerned, I could not distinguish it from Diaporthe parasitica Murr. As the ascospore stage was not present, I did not venture to claim that they were the same species. 7 The writer has since made a careful hunt for Ludothia gyrosa and has specimens of it on two species of oak collected in Cou. necticut and the District of Columbia. Cultures have been made of these, and from Diaporthe parasitica on chestnut obtained from the same localities. Our studies of these cultures and specimens from yarious localities are not yet complete, but they have gone far enough to say definitely that Diaporthe parasitica belongs in the same genus with the Lndothia gyrosa on oak, and at least is very closely related to it, though at present my opinion is that they are distinct species. Professor Farlow has also made further studies, and I have presented his paper on the subject. We have not been able so far to find in literature a reference to Hndothia gyrosa on chestnut in this country before the outbreak of Diaporthe parasitica in 1904. | Neither have we found speci- mens in an herbarium that were collected before that date. We have not, however, quite exhausted all opportunities for investi- cation along this line. If it is ever proved that our Hndothia gyrosa on the oak is exactly the same as Diaporthe parasitica on the chestnut, of course it is at once apparent that Diaporthe para- sitica is a native and not an imported fungus. A second observation that leads me to believe that Diuperthe parasititca is a native species is the fact that frequently in Con- necticut I have found it as a languishing parasite on the roots and base of trees, where it was doing no very apparent harm, and this is somewhat the way Hundothia gyrosa occurs on oak here and elsewhere, and is also the way that the so-called Hn- dothia gyrosa on chestnut acts in Europe, where it causes no particular trouble. This makes me_ believe that these particular occurrences of Diaporthe parasitica in Connecticut represent the fungus in its native condition as an inconspicuous parasite, rather than as an introduced pest that is bound to kill those particular trees. Likewise, I believe that at least part of the so-called spread of the disease in this country is merely an unusual development of the fungus which has existed there for years in an inconspicuous way. A third indication that the chestnut blight is a native species is a comparison of the situation of Hndothia gyrosa in Europe and in this country. In Europe Endothia gyrosa has been re- SO ported on chestnut, oak and various other hosts in different places, but apparently the natural home of the fungus is Soutl- ern Europe, as it has been reported most frequently from Italy and France. In Germany, Winter reported that it produced its pycnidial, but not its perfect stage, though both are found in Italy." Now, if Hndothia gyrosa has a variety of hosts, including chestnut, in Europe, and prefers a southern habitat, what of its preferences in this country? From an examination of literature and of specimens in the New York Botanical Gardens, it is ap- parent that Mudothia gyrosa has been reported much more fre- quently south of Pennsylvania than north of it. For two years, I and others have been looking for it in Connecticut, and only this winter was it found by our forester. This specimen, like those reported by Winter from Germany, has only its pyenidial stage, though this is the time of year to find the asco-stage. /)i- dothia gyrosa las been found on as many hosts in this country as in Hurope, and likewise chiefly from the south. Why may we not then expect to find it there on the chestnut? We certainly] have had trouble enough with the chestnuts in the South in for- mer years to believe that it might occur there.* The second point expressed in my view is that the chestnut} blight fungus is also a native of Europe. Briefly stated, my rea- sons for this belief are: (1) The specimens in deThueman’'s exsiccati on chestnut in Italy already referred to; (2) the state- ment of Professor Farlow that he has seen identical herbarium specimens of it from Europe; and (3) a recent letter from Pro- fessor Saccardo of Italy, who states that he and Professor Hol- nel simultaneously recognized that Diaporthe parasitica Murr. is the same thing as Hndothia gyrosa, both in its ascospore and conidial stages. A critical study of more specimens on all hosts from each country may, however, settle differently some points’ at present not clear to me. *After the Harrisburg conference the writer went South especially to see if Pndothia gyrosa or Diaporthe parasitica occurred there on chestnut, as suggested in this paper, though never having been so reported. Stops were made at Roanoke and Blacksburg, Va., Bristol. Va., and in Ten- nessee and at Asheville and Tryon, North Carolina, and Lynchburg, Va., and at each place there was found the suspected fungus on both chestnut and oak, and more frequently on the former, This fungus occurred asa languishing parasite or as a saprophyte, usually at the base or on the roots of the trees, and was never found forming isolated ecankers on the otherwise sound sprouts, as is Diaporthe parasitica in the North. Apparently this fungus is the same on both the oak and chestnut, and the same thing as the so-called Endothia gyrosa on the same hosts in Eurepe. What its exact relationship is to Diaportha parasitica has not yet been fully determined. In gross ap-| pearance its fruiting pustules are searcely different, except possibly slightly less luxuriant, as a rule. Its pyecnidial spores or Cytospora stage is apparently identical with that of PD. para-| silica, but the asco-spores are evidently as a whole less luxuriant; that is, they are somewhat} smaller, and especially slightly narrower. Whether these differences are those of a strain, yariety, or distinct species, is yet to be determined by cultures, inoculations, and further study. Sl The third point in my theory is that weather and other un- favorable conditions have weakened the vitality of the chestnut in the eastern United States, and that the fungus has developed into prominence because of this. The reasons I have for advocat- ing this theory are as follows: (1). The chestnut blight came into prominence suddenly in 1904, just after the severe winter of 1903-4. From my own ob- servation at that time and since, I know that this winter was un- usually severe on fruit, and to a less extent on shade and forest trees in Connecticut. I am corroborated in my views by the ob- servations of Professor Stone, botanist of the Massachusetts Ex- periment Station, who has made a specialty of the diseases and injuries of shade and forest trees. Various experiment stations and other publications show that the fruit trees in New York, Michigan and Ohio suffered from this, and possibly from subse- quent cold winters. (2). Sinee 1907, speaking particularly for Connecticut, we have had five summers with unusual periods of drought, culmi- nating with that of last season, which lasted from June until about the first of August. I know that these droughts have been hard on forest and shade trees from their weakened condition and from the unusual number that have died. Except in the ease of chestnuts, the death of these trees has been laid directly to the drought, by many observers. I have given somewhat more detailed accounts of these weather conditions in my previous re- ports, and will not dwell further on them here. We have found ‘that chestnut trees on the south and southwest exposures, (and on that side of the trees) where they have suffered most from drought and winter injury, have sometimes developed severe outt- breaks of the blight, while the trees on the more protected nortl- ern exposures in the same vicinity did not. (5). We have found cases of chestnut blight developing more severely in woods suffering from fire injury than in surrounding woods not so injured. It has been our almost universal experi- ence that blight develops first and most severely in the easily in- jured chestnut sprouts from one to ten years old, whose new roots have not yet become thoroughly established, and last on the 6 82 Sm sturdy old seedling trees. Hlow many times we can renew our chestnut woods by sprout growth is a question, but that such trees in time are weakened foresters generally acknowledge. Most of our Connecticut chestnut timber has already been cut over at least two or three times. (4). The unusual spread of the disease in very dry years is contrary to the general experience of fungous troubles, which are favored by moist years; and yet here is a case where the severer the drought, the worse the fungus became. If I am wrong about its relation to weather conditions, what a deluge of trouble we may expect with the return of a few moist years! As to my statement that chestnut blight cannot be eradicated in this country by the cutting out and burning method perhaps no one now thoroughly conversant with the trouble will deny, though there are those that evidently believe it can be controled in this way. Man never yet has eradicated a fungus so widely distributed as this, unaided by nature, and is never likely to un- less he eliminates the host. Professors Stewart and Murrill have given reasons why they believe it is impractical even to try to control the disease. I agree in the main with their contentions. The method that is advocated in the present case aims at the com- plete destruction of the infected trees and in some regions, if I am informed correctly, of the healthy as well. This is a decidedly unusual procedure in the control of plant diseases, since usually we aim to save not only the healthy plants but the infected ones as well. I know of no similar practice, outside of nursery in- spections, except that applied in a few regions for the control] of peach yellows. There the infected trees only are destroyed, but the yellows would kill those any way in a short time. There is, howeyer, no National effort to control peach yellows even in this way and at least one State, Connecticut, that started under authority of law to inspect orchards and to destroy all infected trees, repealed that law after a few vears’ trial. Now as to my last contention: that the disease of itself will eradually decline with the return of a series of years favorable to the chestnut trees. If unfavorable weather conditions for the trees have been the chief cause of the rise of the fungus as an ageressive parasite, favorable weather conditions for the chest- ¢ 83 nut will of course bring about the decline of the fungus, unless it has already attained an unusual and lasting virulence from its present aggressiveness. That chestnuts have in the past in our southern States sulfered from disease or injury of some kind yet unaccounted for, no one who has looked up the literature of the subject can deny. I have gathered together statements of this sort from various sources, but will not take the time to present them here. From the fact that no trained mycologist has studied these outbreaks in the past, and from the further fact that the observers often speak of them by such terms as “blight,” “root rot” and so forth, and did not find insects responsible, I, for one, am open to proof as to their relation to Diaporthe pasasitica, despite the statement of two or three observers who have recently examined trees in the South, that there is no such relationship. Anyway, the chest- nuts have suffered severely in these States at different times dur- ing the past seventy-five years, and have been apparently crowded out of the lower lands, but they still seem to be quite vigorous and abundant in the higher regions of those States, since the chief object of the campaign in fighting Diaporthe para- sitica seems to be to keep it north of the Potomae River in order to preserve the valuable timber said to exist south of it. THE CHAIRMAN: We are now to be favored by hearing an illustrated lecture on Chestnut Culture, the speaker being Pro- fessor Nelson T°. Davis, of Bucknell University, Lewisburg, Pa. CHESTNUT CULTURE. AN ILLUSTRATED LECTURE BY PROFESSOR NELSON F. DAVIS, OF BUCKNELL UNIVERSITY, LEWISBURG, PA. Mr, Chairman, Ladies and Gentlemen: TI wish to take you to-night on a little trip to Irish Valley, situated near Shamokin, Pa. I will take you on this trip by a series of lantern slides. 1 wish to show you to-night what has been done in spite of ene- &4 mies, by Mr. C. K. Sober, who has been working with the Para- gon chestnut since 1896 and 1897. In 1896 Mr. Sober began to eraft the Sober Paragon chestnut, as it is now called, on native chestnut sprouts. He had on his farm in Irish Valley about four hundred acres of waste mountain land. This mountain land he wished to reclaim. It was not suitable for ordinary farm crops. His method was to remove everything and, by means of cleanli- ness, which he obtained by using the grubbing hoe, the saw, the axe, and the pruning knife, and then burning everything, to keep his growth clean. In this way he hoped to keep out the enemies, such as the weevil, and another worse than the weevil, the burr worm. There are two species of the burr worm, one of which is new to scientists. It belong to the genus //olcocera, and has been named. in honor of Mr. Sober, Holcocera Soberii. The other larva, the adult of which is not known, is equally injurious. By means of removing the nuts as soon as they emerge, removing the burrs from the grove and burning the shucks as soon as the nuts are taken out, Mr. Sober on fifty acres has practically re- moved the weevil and burr worm, so that last year the nuts gathered from fifty acres contained scarcely a peck of wormy chestnuts. He has done this by means of cleanliness in every way, and by removing the larvae and not allowing them to mature, In other parts of the grove it has not been possible to do this in every respect, and there the weevil is an enemy. It has been his custom, during the last ten years, to remove every dead limb that has appeared in the four hundred acres and if there was chestnut blight, it has been cut off and burned. An actual count of the chestnut trees now in the grove showed forty-four thousand and thirty-five trees that are bearing, and in addition to those there are others that are not yet matured. By means of these slides I will take you in harvest time over the grove as it now is, and then, by means of other slides which I have taken during the last ten years, show you the various steps that have been taken in developing this grove. If we may have the lantern, we will begin our trip. The first slide is a portrait of Mr. C. K. Sober. (Applause). The next slide represents a portion of a fifty-acre tract, as it appeared when he took possession of it. It was covered with waste wood of various sorts. Very little of this was of any use. A typical cluster of burrs of the Paragon chestnut. at ke Les. C 85 Some could be used, of course, for pulp wood; but notice, among the old stumps, there are a few sprouts coming up. These are sprouts of the native chestuut, and it was upon these sprouts that Mr. Sober conceived the idea of grafting. Of course, it had been done elsewhere, but not upon his four hundred acres. That was the beginning of his Paragon chestnut. As we approach the grove at the present time, you will see the mountain side in Irish Valley from this view. This shows on the hillside from a distance a portion of the four hundred acres, which is now grafted, and from one end of the picture to the other represents a distance of over a mile. In the next view, as we approach the farm, coming near to the buildings, you can see the nature of the surrounding country, the hillside. Back in the centre of the picture, at the top, is ninety acres now grafted to the Paragon chestnut. In the next view we see the farm buildings and, starting from the buildings, we will now visit the grove as it appeared last October. Driving up the road you notice along the roadside everywhere seedlings grafted to the Paragon. They have been transplanted, and all along the road wherever you drive, you will see these trees. As we approach the grove, you can see its condition in this view. This is a portion of the four hundred acres. There are Shown in the view about three hundred acres. Above you see the the mountain side, as this grove would now have been had he not cleared it. A nearer approach to the grove shows the grafted trees, and above them the native chestnut principally. This land was ori- ginally covered with, I suppose, white pine. That was removed and later hard woods came in its place, oak, chestnut, and other hard woods. Now it meant considerable work removing and clearing and grafting these trees, and I wish to show you the various stages as we pass along. | As we enter the grove, it is harvest time, as shown in this view. They are gathering the nuts, which have been placed in bags at this particular portion, so that we are just entering the grove. 86 In the next view, the largest tree at the right is about nine years old. Really the work from 1896 until 1900 consisted in experimenting. When the methods were perfected, the real work began, in 1900. Another portion of the grove shows a tree on which the nuts are maturing. This tree is about eight years old. A branch from that tree shows the nuts almost ripe, just ready to open. If we examine under the trees, many of the burrs are fallen to the ground. You can see the burrs and the nuts in the burrs. It is harvest time and the harvesters are gathering the nuts and placing them in piles, whence they can be hauled to the threshing machine, which will be shown later. The next view shows a normal burr, containing three nuts. I will now show the different stages through which fifty acres of this four hundred have passed. I do not have the photographs taken in 1896 and 97. The photographs 1 have were taken be- ginning with 1903 up to the present time. This view shows the work of removing the brush piles, which were left on the ground. These had to be burned, the logs removed and all the sprouts pro- tected. Every native sprout was protected in every way from fire and from injury, and in the front of this view you see a num- ber of sprouts that have been left. These are ready to be grafted. When the logs are hauled out, these have to be protected; when fires are made, to burn the brush and rubbish, these need to be protected. A sawmill was set up, and what wood was valuable used either for railroad ties, or mine props, or pulpwood, for whatever it could be used, so that it partly paid for clearing. When the sprouts are ready to graft, they are about six feet high. Four sprouts are here shown. The two on this side were cut off about the point where the hand is, and these two were selected because, coming from the stump, they came from lower down and a little farther out and apparently had better roots. So two were selected and two were left. The two were grafted on this side and two left, in case of injury to the other two; so that, if anything happened, the others could be grafted the next season. Old trees were cut down in different parts of the farm. This shows a giant tree that was cut in order that this little sprout at 87 the side might be grafted. This was about two years after the tree Was cul. This shows another tree from which four sprouts were grafted, This was grafted in May, and in June the sprouts were started. Of course, all buds below the graft were removed in order to pre- vent the strength passing iba, cie buds. This view shows the same grafts as they were maturing dur- ing the first summer. Three have started; the fourth was a little slow in starting. Here they are shown after one season’s growth. The roots from the old stump contained lots of nourishment and pushed the growth rapidly, so that during one season the growth that you see took place. This was taken in October. Another view showing one season’s growth, after the leaves had been removed. This shows four sprouts grafted. They are growing together. This is a typical sprout after the first year’s growth. Notice it makes a fan-shaped tree. At this point, sometime during the early spring this limb would be cut off here (indicating), this one and the one at that point, thereby insuring the next year a low crown. The growth is so rapid that frequently the wind would break them off if they were not cut back, so that it is much better to cut them back. The next view shows a grafting outfit. These are the sprouts cut from the Paragon trees, called the “scions,” to be grafted on the native sprouts. This shows the tape, which is waxed, and some of the grafting wax. This is the machine for winding the waxed tape, previous to the beginning of the grafting. The wedge graft was used first. This view shows the method of insertion of the wedge graft. It is then waxed and wound with the waxed tape. The wedge graft was used by professional grafters who were employed in 1897, 1898 and 1899, but only about two per cent. survived. The season is very short during Which this could be used, because the bark separates from the stock so early that the union would not take place. This view shows one of the trees, showing a successful union of the wedge graft. This is one of the oldest trees now to be seen in the grove. 88 This view shows a wedge eraft, one of the original ones, that cid grow. This photograph, I think, was taken in 1903, but only about two per cent. of the grafts in 1897, 98, and 99 lived, so that there are only a few of these surviving. The wedge graft method was consequently abandoned. Then budding was tried. This method you are familiar with. This is the bud to be inserted. It is then inserted, wrapped with wax and covered with the cloth. This method, however, was not successful when used in the grove. A few of them lived. The next view shows such a case; two on either side are buds that did live, and in the centre is a whip graft. Here is one that was suc- cessful. After a time the tree heals up perfectly at the union. This view shows the manner of inserting the knife in the whip graft. It should be inserted at a considerable depth. ‘This one is shown with the top cut off ready for grafting. This is the sprout, on which the graft is to be set. This shows another view of the whip graft, the method that has been successful. This came in 1900, when Mr. Sober person- ally took charge of the grafting. He instructed green men rather than professional grafters and had them use his method, being particular to make the scion fit perfectly to the stock. It is then inserted and driven down so that the tongue holds it at that point; it is cut back a little later, waxed there (indicat- ing) and the bud is allowed to develop. This view shows the completion of it. The stock may be even a little larger than the scion. It is better to have them the same diameter. It is then waxed and wrapped with tape and a little piece of wax put on the top of the scion to keep the moisture in. This is the most successful method with chestnuts. This shows one after the graft has started. This is waxed muslin, which is old muslin that will tear readily as the tree grows, and will remove itself, so that it does not girdle the tree. This is after one year’s growth, the union practically complete all the way around. The next view shows a through section, showing the complete union. Here is the tongue which held them together; and here is another section through. Occasionally they decay at that point. This shows a perfeet union of the whip graft. 89 It is very necessary to keep the buds removed from below the graft. The four grafts shown in this view started, all of them, but the buds below took all the strength from them. The bark has been removed from the three on this side; from the other it has not. The sap, of course, flows along the line of least re- sistance and takes all the strength and the graft dies. The next is a photograph to show the Paragon grafted on oak. The tree is still living. The oak now is smaller in diameter than the chestnut, the chestnut growing faster. This was not very successful; still, it is succecssful to the extent that it lives and bears nuts. A defective union. At that point (indicating) enemies can enter,—fungi and beetles. The wind also will frequently break olf a tree at the point of union, if the union is not perfect. A sprout was allowed to mature on this and later was grafted. The growth is very rapid, and the chestnut not being a strong wood, many were lost in this way where the union was defective. This is a portion of the fifty acres as it appeared six years ago. Ilere we have a view of it a little later. All the roots of the other trees begin to sprout and it is necessary to clean out every- thing. If the underbrush is allowed to grow, it will, sooner or later, choke out the trees and will allow enemies to develop; you cannot keep it too clean. Fires will run through it; so Mr. Sober early found that it was necessary to keep the growth clean. Many parts of it are now clean enough to mow with a lawn mower. In many places the grass is beginning to grow. I wish you would notice how clean the grove is in places. This is the condition soon after the grafting. Then it was necessary to employ from twenty-five to fifty men to clean out and, in order to save the young grafted trees, screens were made. At one time I saw as many as twenty-five of these screens. They were covered with asbestos to protect them from the fire, and the young sprouts that have been grafted are back of the screens. These men are grubbing.out and cleaning, trying to get the eround clean enough to raise grass. This view shows one of the screens a little nearer and some of the men, who rested a moment while I took the photograph. 90 Here they are again. Even after the trees are of this size, it is necessary to finish the cleaning. They are cleaning out every- thing; any suspicious sign, any dead tree, is cut out. Another view showing them carrying the material to the screens for burning,—grubbing out sprouts, so that later it was possible to run a specially constructed mowing machine through it, and much of the undergrowth could be cut off in that way. It is possible to run a mowing machine through nearly all of the four hundred acres, except where there are too many stones, Originally the idea occurred to Mr. Sober to graft the tops of a few of the trees, and we had full sized trees in which eight or ten grafts were set on the top. This view shows one where the top was grafted; this one is another, with the top grafted. That, however, did not prove successful, because you would have only « few limbs in the top, and in a little while the others would catch up with them. Here is another view of a tree grafted at the top, and this a younger tree, two years old, beginning to bear nearly as many nuts as the grafts at the top of the other tree. You can see, therefore, why that method was abandoned. The Paragon be- gins to bear very early, the second year after grafting; occa- sionally the first year a burr or two will mature. Now the grove is beginning to look cleaner. These trees are two or three years old. This was taken in the summer time, in June, before the trees had blossomed. This is a young tree two years from the time the graft was set, really the third summer for it; a typical tree. It is now making independent roots for itself and in a little while it will be free from the old stump. Many of the old stumps are still standing. Some of them have rotted away. Another portion of the grove, just a little later, showing trees one, two, and three years old, and the tops of a few trees that were grafted in the top. This view shows two trees by the roadside, one two years old; the other in the third season of its growth. Notice the shape. They were cut low, so as to secure this low crown, which makes it convenient in harvesting the nuts. It keeps the trees low. It is like it is with a peach tree; the shape is much the same as that of a peach tree. ~ brie pes al hit Group of Paragon chestnut trees, two, four and six years old. 91 This is a view of an ideal tree, three years old, with a low crown, two feet from the ground. In this view, the huckleberries are beginning to grow under- neath; all through the grove the huckleberries have filled prac- tically everything. The ground has been burned over, to burn up the leaves and the burrs, which contain the enemies, and the huckleberries and chestnut sprouts are coming up; but it is nee- essary to keep these down. This view shows how that same ground was cleared, and how it appeared in the winter time. Everything that could be removed was grubbed off and burned, the screens which you saw before being used. This is a young tree, three years old, in the third summer. From that tree three hundred burrs were removed. Two lun- dred were left to mature. This was too many, and many of them dropped otf. The leaves were picked from that same tree, and this view shows it with over a hundred burrs in which the nuts are practically ripe. This is the grove as it appeared in 1904. This is a portion not of the fifty acres, but a portion in the flat which was grafted about 1800, some of it in 1899. Notice now that the grass is be- ginning to grow below the trees. The stumps are there, but the tree is becoming independent. It is now possible to have the mowing done by means of cattle and sheep in the grove. This is another view showing trees out by the edges, as the sprouts come up. They were grafted until there is a stand all over the four hundred acres, and now it is necessary to use the axe to trim out, because they are too thick. Another portion, showing a four-year old tree, with nuts. This view shows the trees a little older. This was taken in 1910 and shows the character of the mountain side. It is coy- ered with stones; impossible to mow around it; it has to be grubbed,—but an ideal place. The stones help to keep the mois- ture in the ground and the trees do exceptionally well. This is another tree, a photograph taken in 1911. It was in October and the nuts were ripening on the tree. The next view shows part of an old hedgerow that had grown up with everything. Stones from the field on either side of this had been thrown along a gully that existed there at one 92 time. In that row, in which there are altogether about twenty- nine trees, three years ago there matured nine and a half bush- els of nuts, just on that old hedgerow, that could not be used for anything else, and full of stones. Some of the trees in that row last fall had almost half a bushel of nuts on single trees. The trees on the four hundred acres last year were practically all bearing; some of them less than a pint to the tree; others almost half a bushel to the tree. This view was taken in 1903, or 1904. Notice the size of the trees, especially. About five hundred sheep were put into the erove and they are doing the mowing and fattening themselves, Where the machine is no longer needed. This view shows the same trees in 1911. The fence by the side shows the growth from 1904 to 1911. They are rapid growers, because they have a good root system. This is a view of the ridge, a portion of the ridge that has a southern front. There are ninety acres grafted, and all bearing. The red spider appeared on this southern side, the sunny slope, and interfered with the growth. Here is a near view on the three hundred acre lot. It shows the condition which might have existed up to the top of the ridge. That is Mr. Sober’s line. A fence is built,—you can just see the posts,—and that fence is a mile long, running from one end to the other, and below it is what you see and above is waste mountain land, containing chestnut and rock oak. Through that, of course, fires run every now and then and it is necessary to establish fire lanes at the upper end, so that below the fence is a fire lane which will prevent a fire from getting into the grove. This photograph shows what was there in 1896 and 1897—that same grove that you saw up at the edge. This has been possi- ble with Mr. Sober, and it is possible anywhere where the chest- nut grows. You can make the change from this to what you saw before. This is the identical spot that you were looking at in the pie- ture preceding. The preceding picture was taken five years ago, and here it is to-day. These trees have been grafted two years and three years, and they are bearing. On this fifty acres ever since they have been bearing, every nut, practically, has *s]nujs ayo uo BR. 1B] 7 oo ulysaty] pure a oO SUMSOATBTT His) been removed, so that last year on this fifty acres there was scarcely a weevil, and scarcely a burr worm, in the nuts that ma- tured there. Another portion, showing young trees bending over, breaking down, almost, on that same fifty acres. It was taken in 1911, about the first of October. The trees are heavily laden, the nuts perfect. Another view showing the sheep doing the mowing. The cat- tle help with the work. Pigs help, but I do not have a photo- eraph showing them. pass an unselfish law. If we could only have a Federal law that would be as broad as the law of Pennsylvania ought to be and could easily be made, by simply in- serting the words after “the chestnut tree blight,” “and any other fungous or insect pest,’ we would have no trouble with our fun- gous or insect pests after a certain length of time. Sometime ago 1 wrote that only when we considered a tree that is dangerously infected with an insect or fungous pest as dangerous as a person infected with smallpox or as a rabid dog, will we get rid in our forests of insect and fungous pests. [I was very glad to hear that Mr: Williams and the members of the Commission have not. be- come discouraged by the large amount of cold water that has been thrown on their plans. I am sure that the two hundred and seventy-five thousand dollars that the State of Pennsylvania has appropriated will never be missed, even if no beneficial results are obtained; but that the everlasting shame that the State of Pennsylvania would suffer if she made no attempt to save her. chestnut trees, should be enough not to discourage any and all citizens from unselfish effort for their fellow men. GEORGE G. ATWOOD, of New York: Mr. Chairman: There is a little desk in Albany that has been open for about a 117 year, and in that desk is about everything that has been said, or thought, or dreamed of, relative to the chestnut bark disease, We have had the advice of our friend Stewart, who thinks as Dr. Clinton does, along the same lines. What they have stated here to-day we must accept as the honest statement of men who know enough to make such statements. They know what they are talking about, because they have investigated this disease and they have investigated similar diseases, so that we must take what they say with a great deal of confidence. They have been talking to the point whether chestnut bark disease could be con- trolled or eradicated. If I were to ask either one of those gentle- men what they would do with a chestnut tree in their own yard that was infected with this disease, they would probably say, “Cut it out.” That gives us the keynote of what I think should be done wherever there is a possibility that single trees, or small infections, can be removed. That seems to be the simple thing, and the proper, sensible thing to do. It may have to be done by the force of statute, but a great deal can be done by advising owners of chestnut trees that become slightly infected, asking, urging, forcing them in every way you can, to cut that timber while it is still alive and save it. If that were done in the State of Pennsylvania, their entire two hundred and seventy-five thou- sand dollars would be well expended. We are up against a proposition in New York. We have probably two-thirds of our chestnut timber still intact, and we want to save it if we can. Now why should we not go out in the borders and carry on a missionary work, or something stronger, and see if we cannot cut a dividing line? Let scientific men go on with their investi- gations. We need all the advice that their broad knowledge can bring to us; but the other thing is a practical thing, a thing that is at our doors, and a few hundred thousand dollars spent now may result in a saving of that valuable property lying all to the west and south of us. (Applause). DR. J. RUSSELL SMITH, of Pennsylvania: Mr. Chairman: Professor Clinton advanced a very interesting point; that it was the dry weather that made these trees amenable to blight. The evidence was that people in Connecticut thought the dry weather had killed other trees that died, if I remember the 118 gentleman correctly. It seems to me that that matter of the drought would be much better tested by showing that, in loeali- ties of low, moist, abundantly watered soils, the trees had not had blight. There must be many such localities of chestnut in Connecticut where even the recent droughts of past years have not subjected many trees to a dearth of water. THE CHAIRMAN: Can you answer that in a word, Pro- fessor Clinton? PROFESSOR CLINTON: I was giving the various things that weaken trees. Drought is one of them. We have had severe droughts in Connecticut, and I hold that the situations that have been the most moist have been the regions that have suffered most from the drought, because when a tree is trained to live in a moist place, during a drought it will suffer more than a tree on higher land which has been used to dry soil. MR. CRANMER, of Pennsylvania: Mr. Chairman: While still well on the sunny side of life’s meridian, I distinctly re- member, as a barefoot boy on a little farm on the eastern sea- board of New Jersey, the advent of what was known then as the Colorado beetle, commonly called the potato bug. As a little boy about this high (indicating) I was put in between the rows to catch those fellows and get them off the vines. Naturally they appeared on the vines of other farmers in that section, and many of the old fellows shook their heads in despair. They said “We will never raise any more potatoes. The potato crops are done in America.” My father did not feel that way, although [ would have been pretty well satisfied if he had. He made me hunt potato bugs, and then we later began to use the London purple and the Paris green, and so forth. We are still raising potatoes in New Jersey and other places throughout the United States, with success. We still have specimens of the Colorado beetle in the United States, but we expect to go on raising pota- toes, and doing our best. So it seems to me, gentlemen, in rela- tion to this chestnut bark blight, this chestnut tree disease, we are not to hold up our hands in despair and listen to too much of the expert advice and opinion that falls from the lips of our university men. I come from a university myself, and I dare say that. We have heard much to-day. There have been numer- 11) ous expressions of opinions and of guesswork. We have yet to hear from any person who tells us what he has done in a practical way for the cutting out and eradication of this dis- ease in any extended form and over any very large tracts of land. JY am unfortunate in the fact that my chief, who is custodian of all the property at Lehigh University, is not able to be here to- night, Dr. Henry 8. Drinker, whose hame appears in the roster of officials of the American Torestry Association, and who is president of Lehigh University. He is custodian of a large tract of land, adorned on its campus with many primeval chestnut monarchs from eighteen inches to three feet in diameter, giants of the old forest tract. In the rear of this campus we haye some two hundred acres covered with a coppice growth of chest- nut and various hardwoods of Pennsylvania. We were exceed- ingly fortunate, some years ago, in having heard from the lips of Mr. C. W. Levitt, an eminent landscape engineer of New York City, the warning that our chestnut trees were likely to be visited with an insidious enemy, which would destroy them all. It was not, however, until the summer of 1908 that I as custodian of those grounds, saw any unusual discoloration on either the bark or foliage of a chestnut tree, except that which seemed to be natural in the decay of any specimen of deciduous trees. Dur- ing that summer I saw, on a small chestnut, this unusual dis- coloration and the appearance of small red or brown pustules. This tree was immediately cut down and portions sent, after all other portions were burned, to Mr. I. C. Williams, Deputy State Commissioner of Forestry of Pennsylvania, who placed it in incubation and pronounced it the chestnut bark blight, or dis- ease. I am not familiar with the scientific name. I was then cautioned by the president to be careful, observant, and vigilant, and to watch for any recurrence of this thing. To hasten from that time on, through the summer of 1910, when it appeared, and in 1911, we have done exactly as was recommended to us by Mr. Williams and by Dr. Rothrock, who visited us during this period of time and walked through our coppice grove of chestnut. I am not able to say, after extended experience along this line, that all trees which are treated by severe -pruning, which have been touched by this blight, may be saved. We do know, however, that we have tided trees over one year and two 120 years, that were striken with the blight, by removing all such portions as were affected by it, treating them with a ‘composi- tion of coal tar, diluted slightly with spirits of turpentine, so that it might be easily applied with a brush, using it both as a fungicide and insecticide; using it on bark, wood, and broken places. Thus far we feel that our work has been successful along this line. Last year it is true we cut out forty trees, all of them less than ten inches in diameter. We have as yet lost but three trees in all this large tract of land that were more than this size. We have, as I said before, saved many trees by severe pruning and trimming, cutting out all diseased places and treating them with this solution of coal tar, ordinary coal gas tar; so that we feel it is worth while to do something along this line. We do not feel like the dear old lady who stood up on the banks of the Hudson River when Mr. Fulton was about to experiment with his steamboat, and said, as it was puffing and * and when the ropes were cast off and the boat moved out into the stream, blowing, “It will never move, it will never move : b) she said “It will never stop, it will never stop.” We hope this will be a suecessful work, prosecuted for the highest end by this worthy and able Commission of the State of Pennsylvania, and we, as representatives of Lehigh University, Dr. Drinker, Professor Hall, of the Department of Biology, and myself as custodian of the grounds, stand ready to help you with anything we can do for you. We stand ready to listen to what you say to us, stand ready to take your advice as a Commission, and go with you hand in hand along this line. (Applause). DR. H. S. REED, of Virginia: Mr. Chairman: Regarding one of Dr. Smith’s questions, we have a few observations upon the chestnuts in Virginia. Reference has been made this after- noon to the blight in Virginia. It has been found there in some instances,—probably there is more there than we think,— but we have observed this that wherever it has been found, that it was at an altitude of less than 800 feet. Most of the chestnut timber that is healthy, and the greatest majority of it, is at an altitude of more than a thousand feet, and on none of that which is more than a thousand feet above the sea level has any trace of the blight been found; but it is found occurring at altitudes less than 800 feet and in regions where the rainfall is great. 121 THE CHAIRMAN: On account of the particularly interest- ing address that we heard from Professor Davis to-night, the Chair thought that there would be some questions directed to him, but it seems that the discussion has gone along on somewhat different lines. There is one question howeyer, which Professor Davis has not answered, with reference to gathering the crop while it is still green, if I remember the question. Will Pro- fessor Davis kindly answer that question? PROFESSOR DAVIS: In September, when the burrs are green, you can shake them from the trees as you can apples, and the entire crop has been harvested without frost. When they are shaken off, they are allowed to dry a little while. When you shake them off in September they color up brown and the frost, I think, has nothing to do with it. THE CHAIRMAN: This note has been sent to the Chair: “Will you please ask Dr. Spalding, of the United States Bureau of Plant Industry, what has been done in the vicinity of Wash- ington, D. C., to prevent the spread of the chestnut bark dis- ease?” Of course, it wiil be impossible now to go into that sub- ject at length, but if Dr. Spaulding will tell us, in a minute or two, something of what has been accomplished, and in a word, the main features of the method, T feel sure it will be appre- ciated. DR. SPAULDING: [Tam not very familiar with the work that has been done in the vicinity, because I have been working on other problems most of the time during the last few years. I simply know, in a rough way, that the method of cutting out had been practiced wherever diseased trees have been found and, as far as I know, that has been fairly successful. There are cases where spores have been found on the stump of an old tree. In many cases, | am sure from Dr. Metcalf’s statement, no special precautions were taken to remove the diseased chips, or eyen to remove the bark from the stump, so that certain cases might very well be expected to have the fungus at this time. THE CHAIRMAN: It seems now, the time being half past ten, that we had best do one of two things: either take a recess until to-morrow morning at sharp nine o'clock, or decide to spend 122 the rest of the night here and finish this subject The Chair learns that Mr. H. P. Marshall is not here and therefore cannot serve on the Committee on Resolutions for New York. He will ask Mr. Merkel to take his place. This Committee will meet at the right of the Chair immediately after adjournment, only for a minute or two. MR. THALHEIMER, of Reading, Pa.: .Mr. Chairman: I was listening to the gentleman from New York. I think he has the proper theory, that is, that the spores are spread by the wind blowing them from place to place, and just according to how the wind blows at a certain time. Take the Orlansa tree. It is called Orlansa in Latin, Lancewood in English and Para- dise tree in German. It is a tree like a sumac. There is prob- ably one out of fifty that has a seed on it, like grapes, and at certain times of the winds they are blown for thousands of feet. Some may land between the mortar, or between the bricks, of a building, and a tree will grow there. If you go up Third street from the ferry after you land there, you will see here and there and everywhere in the front yards a nice little tree growing there, if they have let it grow. You have all seen that, especially in Washington. That seed is just like a leaf, and it is as sharp as a knife, and the seed is encased in that leaf and that gets into any crevice. I shave had some taken out of my wall that grew there, and they would grow to a good size. I have seen them grow out of a brick pavement, where there was not any sweep- ing or any work done around. THE CHAIRMAN: As President Drinker cannot serve on the Committee on Resolutions, Mr. Green is asked to serve in his place. We will now take a recess until nine o’clock to-morrow morn- ing to meet again in this room. (Adjourned until Wednesday, February 21, 1912, at 9 o’clock A. M.) ] wo ~~ MORNING SESSION. Wednesday, February 21, 1912, 9 o’clock A. M. THE CHAIRMAN: The meeting will please be in order. We have a busy session before us and in.a few moments oppor- tunity will be given for the presentation of such business as ought to come up, and then we will proceed with our programme. It has been suggested to the Chairman, and he very heartily ap- proves of the suggestion, that we should start our morning ses- sion with a good taste in our mouths, which would be provided by hearing a few remarks from our old friend, Dr. J. T. Roth- rock, who is recognized as the father of Pennsylvania forestry conservation, and, if there is no objection, the Chair will change from the established order to call upon Dr. Rothrock for a few remarks at this time. (Applause). DR. JOSEPH T. ROTHROCK: Mr. Chairman and Gentle- men: This question of chestnut blight, although of course it is a portion of the forestry work of the State, is somewhat foreign to the line in which I have been most actively interested. I would say, though, that it was my good fortune in 1880 to spend nine months in the laboratory of Professor DeBarry at Strass- burg, Germany. DeBarry at that time was recognized as the leading fungologist of the world. I departed from the faith that was in me then, not because of lack of interest in the field, but because my eyesight gave out, and I drifted then into forestry. So that you will see that I am not wholly without a knowledge of the rudiments of this work that you are engaged in. Now when a contagious disease breaks out among men or among domestic animals, the first thing that is done is to limit, as far as possible, the spread of the infection, or of the contagion. Meanwhile, the laboratories of the land are doing all they can to find out the causes and what is to be done to end the trouble. The two lines of work are progressing side by side. When the Peronospora invaded the vine-growing districts of France and Germany, the laboratories of the Old World were busily en- 124 gaged in finding out how the fungus that produced the trouble in the wine-growing districts found its access into the vines. I had the pleasure of having Professor DebBarry point out to me himself the first spore that I ever saw, sending its germ threads down into the tissue of the plant. I do not know who discovered the Bordeaux mixture, but I do know that that was very in- fluential in limiting the spread of the disease and restoring the wine industry to its normal and natural condition. I do not be- lieve, however, that it was discovered by our scientific friends; but they did discover the life history of the disease, which was a most important, permanent contribution to the vine-growing, Wine-producing industry of the Old World. Now it seems to me that we are in a somewhat similar condition here. We have with us a pest, which is destroying our forests. It seems to me that the proper thing to do is to destroy every spore-produc- ing specimen that we know is actively engaged in disseminating and widening the area of the disease. That would seem to be one commonsense remedy to adopt. It is along the line of what we know in the treatment of contagious and infectious diseases. In the meanwhile, let our laboratory men go on with renewed energy and keep up the work. I think that every State in this Union ought to have a laboratory of well equpiped scientific men, men who follow their work not for their salary but for the love of the work. Those are the men that give you the perma- nent results. I would like to see every State in this Union have a laboratory well equipped and well provided with all that is necessary to produce effective work. Mark Twain on one occasion made the remark that they had a queer way of dealing with criminals out West. He said “They hang them first and try them afterwards.” Now it seems to me that we have the known criminal with us here. Let us hang him first and then let our jaboratory friends try him in the meanwhile. (Applause and laughter). MR. HAROLD PEIRCE, of Pennsylvania: Mr. Chairman: I move that at 11.30 A. M., the Conference take a recess until two o’clock, and at that time, 11.80 A. M., the Committee on Resolutions meet in the House Caueus room. IT would also move that no resolutions be received after 10.30, and that up 125 to that time, all resolutions that are desired to be brought to the Committee on Resolutions be sent to the desk, to be presented to the Resolutions’ Committee. Seconded by Dr. Russell Smith, of Pennsylvania. THE CHAIRMAN: The motion is that we adjourn this meet- ing at 11.30, to reconvene at 2 o’clock, and that at 11.30, the Resolutions’ Committee meet in the House Caucus room, which is below this room, on the main floor, and that no resolutions be received after 10.30 this morning, and that all resolutions should be presented at the desk during the next sixty minutes. You have heard the motion, which has been seconded. Are there any remarks? If there are no remarks, we will call for a vote. The motion was put and unanimously carried. MR. PEIRCE: I have a letter that has been sent me, that I think it would be well to have read. THE CHAIRMAN: Let the Secretary read the letter. Secretary Besley read the following letter, written upon letter head of the Harrisburg Board of Trade: “Dear Mr. Peirce: It occurs to me to suggest that it might be well to haye Mr. Pearson call the attention of the chestnut tree bark disease con- ference to several things relating to the stay of the delegates in Harrisburg. 1. The Capitol Building, itself easily one of the ten great buildings of the world, with its appropriate and memorable art decorations, is an exhibit worth looking at. There are courteous guides at hand to explain to visitors its features. 2. The State Museum, loused in the Library building, just south of the Capitol building, is almost unique in character. It presents an epitome of the life and manufactures of Pennsyl- vania. 3. The City of Harrisburg is a civic exhibit well worth the attention of any visitor to the conference. It has in ten years made more progress, in proportion, than any other city in the United States, toward true civic improvement. Its two-mile-im- proved water front, open to the public; its 55 miles of paved streets; its great park system, including 749 acres, which last 126 year cared for more than a million and a quarter visitors; its notably efficient and pleasing water filtration plant, open to visitors, on Island Park; its dignified city entrance, at Market Street and the river,—all make it worth a look from those in at- tendance upon the conference. I have instructed the secretary of our Board of Trade, Mr. James A. Bell, to present this to vou and to proffer his assistance in connection with any information about the city. Congratulating you on the already apparent success of your splendid work, and on the monumental and unique character of this conference, I am Yours truly, J. HORACE McFARLAND, President.” THE CHAIRMAN: The Chair would suggest that if Presi- dent McFarland will kindly do so, it would be most agreeable if he would be in the ante-room at the close of this session, to meet delegates who desire to secure further information or sug- gestions from him. Certainly his letter is much appreciated. Is there further business to be attended to at this time? One of the first rules of physies is that two objects cannot occupy the same space at the same time. The Chairman is reminded of this rule when he looks at the programme and reflects upon sey- eral requests that have come to him for other matters than those mentioned on the programme to be presented in the short session of this morning. The fact is, we have now just two hours, and a programme which easily could occupy double that time. If mem- bers wish to give instructions for the guidance of the proceed- ings this morning, it might facilitate matters. MR. SHEPPARD: Mr. Chairman: I move you that the Chairman be empowered to confine all discussions to three points upon this morning’s programme, and that all talks on these sub- jects be limited to three minutes. Seconded by Mr. Merkel, of New York. THE CHAIRMAN: The motion is the discussion on this morning’s session shall be confined to the three points on the 127 morning programme, It woud save a little time if Mr. Sheppard would tell us just how he defines those points, there being four papers. MR. SHEPPARD: First, the Pennsylvania programme, the third, the chestnut blight and the future of the forests, and the fourth, the chestnut blight and constructive conservation. The second item (reports of the State Foresters), is one that would be so general that it seems to me we could hardly get very far with it. THE CHAIRMAN: It is moved, then, that we confine dis- cussion to those three subjects, remarks to be limited to three minutes, which, of course, would govern except by exception being made by unanimous consent. PROFESSOR CLINTON, of Connecticut: What is this? A Pennsylvania Conference, or a Conference of the United States? THE CHAIRMAN: Are there other remarks? PROFESSOR HOPIKENS, of Washington: It might be well, Mr. Chairman, to state some additional subjects that are to be presented this morning, to be taken into consideration along this line. We would like to discuss the inseets before we are through. Pen) THI CHAIRMAN: There have been numerous suggestions that we should give some attention to insects. MR. PEIRCE: I think it would be well for that resolution to carry this morning, not in order to cut off discussion, but because the programme this morning was formed for constructive work and for utilization; and I think it would be well if we would carry out that line this morning. An opportunity will be given this afternoon, I should think, for all other subjects to be pre- sented. If we confine ourselves to the one thing that is specially mentioned in those three subjects, I think we can get more effec- tive work that if we try to have a diverse discussion this morn- ing. THE CHAIRMAN: If you observe the subjects on the pro- gramme I think you will find that they would not confine discus- sion to Pennsylvania questions. Are there further remarks? 128 MR. CASSELL, of Pennsylvania: Do you think it might be well, under the circumstances, to make No. 2 on your programme No. 4? Then, if we have time for it, it could come up and some of our friends, who have come prepared to report under that, would have their opportunity. THE CHAIRMAN: Do you offer that as an amendment? MR. CASSELL: Yes, sir. THE CHAIRMAN: An amendment is offered, that question No. 2 follow No. 4. Is the amendment seconded? The amendment was seconded by Mi. Peirce. THE CHAIRMAN: Do you wish to take any further action, or suggest any further action? If not, we will put the amend- ment first. The amendment was put and carried. THE CHAIRMAN: Now you have the original motion as amended, that discussion be strictly confined to the three sub- jects. Is there any desire to open up the insect question this morning? If so, we should hear another amendment. PROFESSOR CLINTON: I understand that Mr. Hopkins has something to say, and I, for one, should like to hear what he has to say. I move that, at sometime at least, we hear from him. I do not care whether it is this morning or this after- noon. THE CHAIRMAN: The Chair would be glad to entertain an amendment. PROFESSOR RANE, of Massachusetts: It seems to me that we are losing a good deal of time on these amendments. I should like to hear the papers, and then also hear Professor Hopkins on the insect question. MR. PEIRCE: TI would move that Professor Hopkins pre- sent his paper at two o'clock this afternoon. The motion was seconded. 129 THE CHAIRMAN: The Chair has one motion before the House, to confine the discussion to three subjects and remarks to three minutes in each case. (The motion was put and carried). THE CHAIRMAN: Mr. Peirce makes a motion that Profes- sor Hopkins be requested to speak on the insect question at two o'clock this afternoon. The motion was seconded by Mr. I. C. Williams, was put and duly carried. THE CHAIRMAN: Having executed the criminal, we will proceed with the trial, and ask Mr. Hopkins if that will be agree- able to him. PROFESSOR HOPKINS: I had planned to leave for Wash- ington directly after dinner, at least at three o'clock, and I am afraid that will interfere with my plans; but, if it is the wish of the meeting, I will submit. THE CHAIRMAN: It would be very kind of Professor Hop- kins to remain over. It seems almost the unanimous wish. We will proceed with the morning programme, the first paper being “The Pennsylvania Programme,” by the first secretary of this Conference and the executive officer of the Chestnut Blight Com- mission, Mr. 8. B. Detwiler. (Applause). THE PENNSYLVANIA PROGRAMME. By 8S. B. DETWILER, EXECUTIVE OFFICER, PENNSYLVANIA CHEST- NUT TREE BLIGHT COMMISSION. Mr. Chairman, Ladies and Gentlemen: Although a deter- mined effort to control and eradicate the chestnut bark disease was made by a number of public spirited citizens, residing in the vicinity of Philadelphia, it soon become evident that they were unable through individual efforts, to save their valuable chestnut trees from destruction. As a result, Pennsylvania took up the fight against this destructive tree disease in earnest, realizing the necessity for prompt and vigorous action on the part of the Commonwealth. A Commission was appointed in 9 15¢ June, 1911, for the purpose of thoroughly investigating the chestnut blight, to devise and apply ways and means through which it might, if possible, be stamped out. In 1909, according to the report of the State Auditor General, there were 7,633,180 acres of forest land in Pennsylvania, of which it is estimated that 21 per cent., or approximately one- fifth, is chestnut timber. Allowing two poles, four ties, and two cords of wood per acre, and allowing $2.00 per pole, 53 cents per tie, $1.00 per cord for wood, the total value of the chestnut timber in Pennsylvania would be $55,000,000, in round numbers. If we allow $15,000,000 as the total value of the nut crop, and orchard, park, and shade trees, the total value becomes $70,000,- 000. This does not consider the value of chestnut forests as protection for water-sheds. By dividing the counties in the eastern half of the State into zones, as shown on the map, on the same basis as the above estimate is made, the value of the chestnut trees already killed or affected by blight in Pennsyl- Vania is estimated at $10,000,000. Of this amount $7,000,000 is the value of poles, ties, and other wood products, and $3,000.- 000 is estimated as the value of orchard, park, and shade trees, the loss to nurserymen, and to real estate owners. It is believed that $3,000,000 is a low estimate for the value of these trees, since the loss to real estate owners and to owners of shade and orchard trees has been particularly severe in the southeastern corner of the State where the chestnut tree is of great importance in this respect. No reliable estimate of the annual income from the sale of chestnut products in Pennsylvania can be given. The statistics of the Forest Service, for the vear 1909, show that for the United States, the value of the annual cut in that vear was approxi- mately $20,000,000. Of this amount, about one-half was the value of lumber, lath, and shingles, the other half representing the value of poles, ties, and extract wood. The Pennsylvania Chestnut Tree Blight Commission began its investigations in August, 1911. The general plan adopted by the Commission is that recommended by Dr. Metcalf in his recent bulletin on the control of the chestnut bark disease. In brief, this consists in first determining the exact range of the Il disease, especially the advance points of the infection. The dis- eased trees of these spot infections are destroyed as soon as possible after being located. Ultimately, it is planned to es- tablish a zone free from the disease which will be constantly patrolled for new infections. The portion of the State west of this zone will be thoroughly scouted over at least once each year and new spot infections eradicated as soon as found. Kast of the immune zone no immediate attempt will be made to eradicate the disease, partly because most of the energy will be required to fight the disease in the immune zone and west- ward, and also because of the poor market for chestnut pro- ducts, especially cordwood, of which a large amount will be pro- duced. It is planned, however, to place competent men in the region of general infection for the purpose of encouraging tim- ber owners to cut their diseased trees before they deteriorate, and to assist them in finding a market for this material. In com- munities east of the general advance line where the per cent. of blight is not high and the owners desire to co-operate in cutting out the diseased trees, the Commission plans to give all possible encouragement and assistance. At the risk of being tedious, I will give a resume of the pro- visions of the Act which governs the work of the Pennsylvania Conunission. Section 1. A commission consisting of five persons, to serve for three years, is created. They are given power to use all practical means to destroy the chestnut tree blight. The Department of Forestry is directed to work in collabora- tion, Section 2. The Commission and its agents or employes are given power to enter upon any property to determine whether trees are attacked by blight. They are directed to co-operate with owners for the removal of the trees and eradication of the disease. The commission will furnish every owner with infor- mation respecting the location of his blighted trees. Section 3. If an owner refuses to co-operate with the Com- mission in applying remedies or doing any act directed to be done to prevent further spread, the Commission may give him 152 twenty days’ notice that it will proceed if he does not. At the end of the period of notice the Commission may cause trees to be destroyed and the cost of doing such work is collectible from the owner; and if the cost be not paid within sixty days, the Commission is directed to proceed by action at law. An owner may appeal from the decision of a member of the Commission or any of its agents or employes, within ten days after receiving his notice. The Commission will then direct a re-examination and accord a hearing to the person making the appeal. Proceedings in the meanwhile will stay. Section 4. The Commission is given power to establish a quarantine or destroy trees not affected by blight, if so doing will result in preventing spread of the disease. Good trees so de- stroyed are to be paid for at current stumpage prices. In case an owner be dissatisfied with an amount allowed him for the destruction of good trees, he may appeal to a court for such remedy as he thinks he may be entitled to. Section 5. Violations of this Act or any of the regulations adopted by the Commission, or resistance to an officer of the Commission, are declared to be a misdemeanor, and upon con- viction, the defendant may be fined $100 or imprisoned one month; and the provisions of the Act are extended to corpora- tions as to individuals. Section 6. The Commission shall receive no pay but actual expenses only. The employes of the Cominission are to receive such compensation as the Commission may determine. The superintendent of Buildings and Grounds shall furnish them with suitable offices. Twenty-five thousand dollars is appropriated for scientific re- search and office expenses, and $250,000 additional for general field work. Section 7. Repeals all inconsistent legislation. A quarantine on the shipment of chestnut nursery stock was declared by the Commission soon after its organization. Regu- lations were made requiring that all nursery stock prior to ship- ment be inspected by an agent of the Commission and dipped for several minutes in an approved fungicide, preferably Bordeaux mixture, in the presence. ofan inspector. Nurserymen are pro- hibited from shipping, and transportation Companies from carry- ing chestnut stock not bearing the Commission's tag. Chestnut nursery stock shipped into the State from without is to be held at the border of the State for inspection. The nurserymen and transportation companies of the State deserve credit for will- ingly co-operating with the Commission to make this regulation effective. A field force of over thirty men has been organized and the extent of the blight in the State has been determined approxi- mately. The infected region in Pennsylvania occupies the east- ern two-fifths of the State. The western-most line of general advance may be shown by drawing a line from Susquehanna to Williamsport, and southward through Huntingdon to the south- ern boundary of the State, although there are scattered spot in- fections west of this to near the Ohio State line, in the south- western corner of the State. The field work done by the Com- mission last summer and fall was largely scouting to locate the extent of the disease. From January 15 to February 15, 1912, 1,552 infected trees on 87 tracts have been disposed of according to the regulations of the Commission, and fully as many more are in the process of removal. This is part of the work, in ad- dition to general scouting and the holding of meetings for the purpose of educational work on the part of the field agents. During the summer months, when the work is carried on to the best advantage, it is planned to increase the field force so that the State may be thoroughly scouted and all diseased trees cut out west of the advance line. On the advance line and to the westward, the owner of the trees marked for removal is required to burn the bark from visibly diseased or cankerous portions of the trees. He is also required to destroy the bark of the stumps of infected trees, either by peeling the bark to the ground line and burning it, or by burning the brush over the stump until the bark is consumed. Iixperiments are being tried to determine if it is not practical to cover the stump with kerosene, crude petroleum, tar, or some similar material, to make the destruction of the bark thorough and less expensive. ial 134 the year can peel six stumps 10 to 15 inches in diameter in an hour. That is a conservative figure. It is the policy of the Commission to use every possible means of securing the co-operation of Owners in cutting infected tim- ber, before resorting to their power under the law. The power that the law gives the Commission is sufficient to insure respect for its powers, but we realize that the law alone is not suflicient to make the plan of controlling this disease effective unless it is backed by strong public sentiment in its favor. This is being | accomplished by educating the public to recognize the symptoms of the disease and to realize its serious character through lee- tures, field meetings, circulars, newspaper articles, and other work of an educational nature, such as interesting school chil- dren and boy scouts in the movement. So far, no serious oppo- sition has been met with in the work of eradication; on the con- trary, we have had exceptional co-operation from all classes of timber owners. The Commission maintains a laboratory for determining doubtful infections, and for conducting experiments in the con- trol of the disease through the use of sprays, fertilizers, and medi- cations. The Commission is giving an impartial trial to the many remedies submitted, to determine their effectiveness. These ex- periments are being pushed forward as rapidly as may be done, but no remedy will be endorsed by the Commission until its efficiency has been demonstrated beyond all doubt. Most of those submitting remedies for the blight have in mind the size of our appropriation rather than the practicability and efliciency of their remedies to the public. The Commission keenly realizes its responsibility to the pub- lic for the proper expenditure of the funds placed at its disposal. Yesterday's proceedings of the conference emphasized the great need for comprehensive scientific investigation into all phases of the blight problem. It is only by finding out all the facts relative to the disease that we can hope to eradicate it, and it is evident that many scientific facts of practical importance are still unknown. Tor instance, it has not yet been definitely deter- mined what agents are of primary importance in distributing the spores, or to what extent the disease may be spread by the 135 transportation of barked and unbarked products of diseased trees, two points which have a direct bearing on cost and efliciency of control. The woodpecker and other birds have been blamed for spread- ing the blight, when in my opinion it is more apt to be the fault of insects. Further investigations may prove this to be as much a problem for the entomologist as for the pathologist. We feel a sentimental interest in the birds. Nevertheless, this does not free us from also investigating them to find out scientifically their exact relation to the spread of this disease. In other words, we must investigate everything, whether we believe one thing or another. At the present time three field agents have been de- tailed to make special studies of field conditions for the purpose of securing further facts relative to several of these problems. Many lines of co-operative investigation and experiment are in progress and others are planned. Detailed knowledge of the agents causing infection and the time of year when infection occurs, Which will be obtained as the work progresses, will un- doubtedly assist in making control more etfective and in cheapen- ing the cost of the work of eradication, by pointing out the simplest methods required to give satisfactory results. In the meantime, however, it is our belief that sanitation is practical and should give good results in checking the spread of this dis- ease as it has done in the case of other diseases. Quarantine measures proved successful in checking outbreaks of yellow fever after the mosquito was convicted. It is more than prob- able that by destroying the diseased bark of infected trees in the eastern half of the State, we shall also destroy the agency which spreads the disease. In my opinion, the big problem which confronts us and which more than any other will determine the success or failure of our undertaking is the question of profitable utilization.