Historic, archived document Do not assume content reflects current scientific knowledge, policies, or practices. Diet as a Factor in Length of Life and in Structure and Composition of Tissues of the Rat with Aging — Home Economics Research Report No. 24 ae Agricultural Research Service . UNITED STATES DEPARTMENT OF AGRICULTURE — Diet as a Factor in Length of Life and in Structure and Composition of Tissues of the Rat with Aging by > Mildred Adams | Human Nutrition Research Division Agricultural Research Service UNITED STATES DEPARTMENT OF AGRICULTURE Home Economics Research Report No. 24 Washington, D.C. Issued October 1964 For sale by the Superintendent of Documents, U.S. Government Printing Office Washington, D.C. 20402 © Price 70 cents Preface This research was done as part of a project supported by an allotment made by the Secretary of Agriculture from Special Research Funds (Bank- head-Jones Act of June 29, 1935). Staff members in the Human Nutrition Re- search Division responsible for the results reported in this publication were as follows: Mildred Adams, Research Chemist. Organi- zation and evaluation of data; preparation of report. Elizabeth C. Hartman,! Nutrition Physiologist. Initiation of this investigation ; general supervision for the longevity studies. Anna M. Allen Durand, Medical Officer (His- tology). Evaluation of histological findings in the tissues. Murray Fisher, Biologist. General supervision of experimental animals; collection of data on organ weights; evaluation of gross findings at necropsy; preparation of tissues for histological examination. Doris D. Taylor, Nutrition Specialist, and Emily 8S. Conway, Biological Aid (Gen.). Chemi- cal analysis of diets, livers, kidneys, and serum cholesterol. Mrs. Conway also assisted in com- piling and tabulating the data. Hazel E. Hildebrand, Nutrition Specialist. Calorie determinations. Florence L. Lakshmanan, Donald Higginbotham,’ phoretic analysis of serum. Mention of specific products throughout this publication does not imply recommendation by the U.S. Department of Agriculture over other products of a similar nature not mentioned. Biochemist, and Biochemist. Electro- 1 Present address: Chief, Training Grants and Awards Branch, Extramural Programs, National Institute of Neurological Diseases and Blindness, National Institutes of Health, Bethesda, Md. 2 Present address: E. I. duPont deNemours & Co., as Fibers Department, Dacron Division, Kinston, N.C. ENxpenlment al Maes) s eee eae See a ee ae Description and management of animals_-_-_--- DiCtS me ea eee eee ee ee eee Criteria for evaluating response to diet__-_---- Results and discussion_______________---------- IO oa fF w Composition of experimental diets_____-_---- Proteins aan eee oe ee eee ae eee Gi (aes See eae ee ee ee eS Body weight, intake, and age (stock, SP 8 HVO, and SPE diets) __________-_--_- Growth and food intake of young rats (all Gilets) eee Ake ote Woes hoo Sed Body weight in relation to age and diet in adulijratsse" 28e eos See! 22 oo eee Calories for maintenance of body weight _- Maximum body weight and diet________- Experimental Diets . Diet codes and description of experimental CLIC ES eae atts Cee Maal Siok, coer S es Be ay . Protein and amino acid content per 100 grams of diet, and suggested requirements for the DE sng ee Lg a . Fatty acid content per 100 grams of diet and iodine value of dietary fat______----------- . Fat, fatty acid, and cholesterol content per 100 PTAMSHOMGiCtH ee es oe ea eee Vitamin content of diets, and suggested require- mentsiorthe:rats £2 7 #42222 25-2 e . Ash and mineral content per 100 grams of diet, and suggested requirements for the rat__-__- . Calorie values per 100 grams of diet, and per- centage of calories as carbohydrate, fat, and T OVOR HEY BO pee ee pg Body Weight . Growth, food intake, and calories per gram of gain in young rats fed various diets__--_--- . Body weight of rats at different ages fed various CIS ES mate ae Same we Ue Pea Le ee = . Calories per gram of body weight per week for maintenance of adult rats fed various diets_- . Maximum weight of rats fed various diets, sac- rificed before or after 500 days of age__---_-- . Age at death and mortality rate of rats fed VATLOUS GICtS a een ee eee eee ee eS Contents a) © og o OOO OOo > NR ar —~ = an co — lo — wee) oo Results and discussion—Continued Body weight and longevity as influenced by diet— Continued Histology of liver__.__..__._.._....._____- Kidney and liver weight________________ Adrenal weight_______________________- Thyroid weights: = 2 3-2 ee Thymus weight Chemical investigations____________________ IG eh iYehi eee en een Serum protein components_____________- General summary and implications for future re- SCAT Chase ome Sate ee oe ea ae a ed ns he eR ee HN 0) {SOG Bb Cee ke a Se eg is a ee ate Tables Page 13. 14. 15. 16. Body Weight—Continued Maximum age of rats before weight loss in relation to caloric intake during the first 300 days on SP 8 HVO and SPE diets______ Histology Kidney damage determined microscopically for rats maintaining weight at different ages on stock, SP 8 HVO, and SPE diets________ Kidney damage determined microscopically for rats losing weight at different ages on stock, SP 8 HVO, and SPE diets__--__-_--- Calcium determined microscopically in kidneys of fasted and nonfasted rats losing weight at different ages on SPE diet____-_-_------ . Kidney damage determined microscopically for rats maintaining or losing weight at different ages on SPM, SPB, and SPPB diets______-- . Kidney damage determined microscopically for rats fed all other diets__.________--____-_--- . Liver fat determined microscopically for fasted and nonfasted rats maintaining or losing weight at different ages on SPE diet___-__-- . Liver fat determined microscopically for rats fed SPM, SPB, and SPPB diets___--------- . Liver fat determined microscopically for rats fed other experimental diets__-_----------- Page Page 30 44, 45. Kidney and Liver Weight . Kidney weights in fasted and nonfasted rats maintaining weight at different ages on stock, SP 8 HVO, and SPE diets_____...__.-__-- . Liver weights in fasted and nonfasted rats main- taining weight at different ages on stock, SP 8 HVO, and SPE diets____..__-__________ . Correlation of liver, kidney, and body weights in fasted and nonfasted rats fed stock and SPS HVO dietss cc. 26 ee . Kidney weights in fasted and nonfasted rats losing weight at different ages on stock, SP 8 HYVO, and SPE diets...-.-2.-.<22shs52.38.- . Liver weights in fasted and nonfasted rats losing weight at different ages on stock, SP 8 HVO, SandsbP Hi diets.. ios Joke . Kidney weight as related to kind and extent of damage in fasted and nonfasted rats fed stock, SP 8 HVO, and SPE diets__________ . Liver weight as related to kind and extent of kidney damage in nonfasted rats maintaining weirht onistock diceti2- 2. 25o.c2 4 252222 . Kidney and liver weights of fasted and non- fasted rats maintaining weight at different ages on diets with protein-fat-containing 1TOO0S nes. SoS a Se ee . Kidney and liver weights of fasted and non- fasted rats losing weight at different ages on SPM, SPB, and SPPB diets... 02-22.2.-.- . Kidney and liver weights of rats losing weight on SPE diet containing added purified nu- (PlentSe se Se See ae eee ee . Kidney and liver weights of fasted and non- fasted rats maintaining or losing weight on diets containing different kinds and levels of on SP 8 HVO diet modified to contain the protein and fat level of SPE diet__-__-_-__- . Kidney and liver weights of rats fed various diets containing egg, egg yolk, or egg white__ . Kidney and liver weights of rats fed stock or SPE diets reversed at 250 days__________-- . Kidney and liver weights in two strains of rats at terminal age from parents fed two stock diets, with young fed SP 8 HVO and SPE lets sec bowed se a ee oe Seo Adrenal Weight . Adrenal weights of rats maintaining weight at different ages on stock, SP 8 HVO, and SPE CN GUS ea coe ee So ae ene a i lene . Adrenal weight in relation to body weight of rats fed stock, SP 8 HVO, and SPE diets__ . Adrenal weights of rats losing weight at different ages on stock, SP 8 HVO, and SPE diets____ . Adrenal weight in relation to extent and kind of kidney damage in rats maintaining or losing weight on stock, SP 8 HVO, or SPE diets. __ . Adrenal weights of rats maintaining weight at different ages on SPM, SPB, and SPPB diets_ . Adrenal weights of rats losing weight in two age eroups fed SPM, SPB, and SPPB diets___-_-_ . Adrenal weights of rats fed other experimental ANGUS hts i Roe tee ERA Le nee eS Thyroid Weignt Thyroid weights of rats maintaining weight at different ages on stock, SP 8 HVO, and SPE @lets..2% 22. 8 Nene Sn en Ron - dees Bae ee Thyroid weight in relation to body weight of rats maintaining weight on stock, SP 8 HVO, and SEH dlets 222242222 eee ee ee Page 44 45 46 47 48 49 49 49 50 57 58 46. 47. 48. 49. 50. 55. 63. 64. 65. Thyroid Weight—Continued, , and SPE of kidney damage for rats maintaining or oe weight on stock, SP 8 HVO, and SPE iets Ds a a a Thymus Thymus weights of rats at different ages on stock, SP 8 HVO, SPE, SPM, SPB, and SPPB diets Kidney Chemistry . Protein, fat, and ash in kidneys from rats main- taining or losing weight at different ages on stock, SP 8 HVO, and SPE diets . Protein, fat, and ash in kidneys of different weights from rats fed stock, SP 8 HVO, ang OS PE diets’: = 22 .etee see oe ee . Protein, fat, and ash in kidneys from rats maintaining or losing weight on SPM, SPB, and SPPB diets . Protein, fat, and ash in kidneys from rats fed other experimental diets Urinary Protein Urinary protein of fasted and nonfasted rats at different ages on various diets__.________ Liver Chemistry . Protein, fat, and ash in livers from rats main- taining weight at different ages on stock, SP 8 HVO, and SPE diets_.__.....______- . Protein, fat, and ash in livers of different weights from fasted and nonfasted rats fed stock, SP 8 HVO, and SPE diets___________ . Liver fat and body weight of fasted rats main- taining weight on SP 8 HVO diet__________ . Protein, fat, and ash in livers from fasted and nonfasted rats losing weight on stock, SP 8 EV ©) and S:Pidietss. 200 2ee- = eee . Kidney damage and liver fat in nonfasted rats losing weight on SP 8 HVO diet___________ . Protein, fat, and ash in livers from fasted rats maintaining weight on SPM, SPB, and SPPB diets). 22. emes-4sutunoce steers . Liver fat in rats of different body weight maintaining weight on SPM, SPB, and SPPB diets 2. 222 5. oe ae ee ee ee Protein, fat, and ash in livers from fasted and nonfasted rats losing weight on SPM, SPB, and S PPB idiets22:52 252 ee eee Protein, fat, and ash in livers from rats fed other experimental diets___--._.----------- Serum Cholesterol Influence of diet and age on serum cholesterol Page 58 59 60 61 63 64 65 66 67 78 66. 67. 68. 69. 70. 71. 72, 73. PF WwW WN Serum Cholesterol—Continued Influence of diet and age on serum cholesterol levels, in rats losing weight on stock, SP 8 HVO, SPE, SPM, SPB, and SPPB diets___- Serum cholesterol levels of rats with kidneys of different weights on stock, SP 8 HVO, SPH, SPM, SPB, and SPPB diets______________- Serum cholesterol levels of rats with normal kid- neys at different ages on SP 8 HVO diet____ Serum cholesterol levels and organ weights for rat littermates fed SPE diet______________-_ Serum cholesterol levels in rats fed other experi- mental diets: 2. 34 sthee2 oat eo. eet ee Serum Protein Protein components of sera from rats maintain- ing weight at different ages on stock, SP 8 EVO-andiSPH diets: 222225202. 55-22=. =. Protein components of sera from rats losing weight at different ages on stock, SP 8 HVO, and: SP Eidietss=) 22. =2-- Lo 2fh22 b22e25 5 = PA, serum cholesterol, kidney weight, and kind and extent of kidney damage in rats fed SP 8 HVO and SPE diets_......_...__.__.__.--.._- . Average weight gain and food intake of rats fed SP 8 HVO and SPH diets_________________-_ . Weight in relation to age of selected individual rats fed SP 8 HVO and SPE diets__________ . Average weight in relation to age of rats fed stock, SP 8 HVO, and SPE diets__--________ . Percentage of total number of rats dying within different age intervals on SP 8 HVO and SPE GILG GS ta reyatat ee ites al eieens Ty eo tet ok . Percentage of total number of rats dying within Page 79 80 80 81 81 87 88 89 Serum Protein—Continued 74. Protein components of sera from rats at differ- ent ages on SPM, SPB, and SPPB diets___- Appendix Tables 75. Protein and amino acid composition per 100 grams of ingredients in experimental diets__ 76. Lipid composition per 100 grams of crude fat and of ingredients in experimental diets_-_-__- 77. Vitamin composition per 100 grams of ingre- dients in experimental diets_____________-_- 78. Ash and mineral composition per 100 grams of ingredients in experimental diets___________ 79. Weekly weight gain and food intake of rats fed SP 8 HVO, SPE, SPM, SPB, and SPPB diets for first Ziweeksaeeees a2 8-2-2 ees 80. Weight, weight gain, and food intake of rats for 100-day intervals on SP 8 HVO, SPE, SPM, SPB and SPPB diets: 2-22-22 22222 22222 81. Body weight, food intake, survival, and organ weights of individual BHE nonfasted rats fed stock and SPE diets throughout life or reversed at 250 days____-_---__---------- Figures Page 12 13 13 24 different age intervals on SPM, SPB, and SRP Bidictse" sesso eae eee ea ee 6. Comparison of growth curves of obese rats fed SP 8 HVO and SPE diets with those of long-lived rats fed the same diets____.-_------------- 7. Comparison of growth curves of obese rats fed SPM, SPB, and SPPB diets with those of long- lived rats fed the same diets_-_-_----------- 8. Liver weight in relation to kidney weight in fasted and nonfasted rats fed stock diet_____-_ Page 90 102 103 104 105 106 107 108 Page Diet as a Factor in Length of Life and in Structure and Composition of Tissues of the Rat with Aging By Mitprep ApAms Human Nutrition Research Division, Agricultural Research Service Early investigations with the laboratory rat as experimental animal have dealt with nutritional factors important for normal growth and develop- ment of the young animal, and have provided much information of basic importance to human nutrition. In recent years, increasing emphasis has been placed on the need for information con- cerning the requirements of adult animals at various stages of their lifespan. In this laboratory, investigations have been underway for several years to determine the influence of various dietary combinations on the length of life and on the appearance of changes in the structure and composition of tissues of the rat. 10 percent. SPBBa22 222424 SP 8 HVO, 80 percent + peanut but- ter,® 20 percent._ SP 16 HVO____| SP 8 HVO with HVO level increased to 16 percent and sucrose decreased to 44 percent. SP’ 8 lards—.— 2 SP 8 HVO with lard? replacing HVO. SP 16 lard_____ SP 16 HVO with lard replacing HVO. SP 8 butter___-_ SP 16 butter___ SPa 16 HVO__- SPb 8 HVO_-_-- Y97-+salt mixture______ SP 8 HVO with butter * replacing HVO. SP 16 HVO with butter replacing HVO. SP 16 HVO with casein increased to 20 percent, lactalbumin increased to 10 percent, and sucrose decreased to 38 percent. SP 8 HVO with casein increased to 20 percent, lactalbumin increased to 10 percent, and sucrose decreased to 46 percent. SP 8 HVO, 86 percent, except HVO, 15 percent; sucrose, 41 percent; and egg white,’ 10 percent. SP 8 HVO, 66 percent, except no HVO; sucrose, 43 percent; and egg yolk,$ 30 percent. SP 8 HVO with egg white, 24 percent, replacing casein and lactalbumin. Whole commercially dried egg, 100 percent. Fresh egg yolk, cooked and dried, 100 percent. Y100, 97 percent, + salt mixture, 3 percent. 1 Whole egg, spray dried, from Henningsen Bros., Inc. 2 Starlac, from Borden Co. 3 Butter washed free of protein and _ salt. Husbandry Research Animal Division, Agricultural Research Service, Beltsville, Md. 4 Beef rounds from dual-purpose cattle with all visible fat removed. Meat Quality Laboratory, Animal Hus- bandry Research Division, Agricultural Research Service, Beltsville, Md. 5 Beef suet from kidney area. Meat Quality Laboratory, Animal Husbandry Research Division, Agricultural Research Service, Beltsville, Md. 6 Peanut butter from two sources: (1) Prepared from roasted white Spanish peanuts by Southern Utilization Research Laboratory in accordance with directions published by the Laboratory (18). The peanut butter contained 1.2 to 1.3 percent commercial hydrogenated peanut oil (Onesta Hardener, Procter and Gamble Co.). (2) Peter Pan Peanut Butter containing salt was used for most of the investigations. 7 Lard. Animal Husbandry Research Division, Agri- cultural Research Service, Beltsville, Md. 8 Prepared in the laboratory from hard-boiled eggs. jacketed kettle, for 30 minutes. The beef was then ground, dried, and reground to produce a fine powder for blending with other ingredients of the diet. The beef suet was rendered and refrigerated until used. For diets containing egg yolks or whites, hard- boiled eggs were used. Yolks and whites were separated, ground, and dried at 150° F. The whites were reground after drying. The diet containing a high level of egg yolk was reground after mixing. Cholesterol or vitamin supplements, except for choline, were added dry and mixed with the SPE diet by blending in the Hobart mixer. Choline was dissolved in water first and then added to the dry ingredients before final blending. The diets were prepared fresh at least every 2 weeks and were kept refrigerated until used. All of the fats and the ingredients containing fat were kept under refrigeration. Aliquots of the dietary ingredients and of the experimental diets were kept for chemical analysis. The dietary ingredients and the experimental diets were analyzed for moisture, nitrogen, fat, and ash. Moisture was determined by drying in a vacuum oven at 70° C. Ash values were obtained by incineration in a muffle furnace at 575° CC. Nitrogen was determined by the Kjeldahl-Wilfarth-Gunning method using mercury as a catalyst (12) and distilling into boric acid (175). Protein values were obtained by applying to the nitrogen values the factors indicated. Fat was determined by a modification’ of the AOAC acid hydrolysis procedure (13). Carbo- hydrate values were calculated by subtracting the weight of fat, protein, and ash from the total dry weight. Mineral components were deter- mined by spectrochemical analysis for the various dietary ingredients and for most of the diets. Those diets not actually analyzed were calculated from the values for the dietary ingredients. Gross calorie values were obtained for some of the diets by use of the Parr Bomb Adiabatic Calorimeter; others were calculated by application of appropriate gross calorie values for the proteins, fats, and carbohydrates which they contained. The values for thiamine, riboflavin, niacin, pyri- doxine, folic acid, pantothenic acid, vitamin By, choline, essential and nonessential amino acids, fatty acids, and cholesterol were all calculated. Criteria for Evaluating Response to Diet Physical measurements, histological examina- tion of the tissues, and limited chemical analyses of liver, kidney, blood serum, and urine provided the criteria used for evaluating the changes occurring in the rat with age and/or with diet. Measurements obtained on BHE rats fed the stock diet provided information on the charac- 7 Unpublished. teristics of the rats that served as a source of the experimental animals. A group of rats fed SP 8 HVO diet was included for comparative purposes in each of the experimental series alinged chiefly with modifications of this diet. Rats fed SPE diet served as a basis of comparison for the series of rats fed SPE diets containing various supplements and for those fed high levels of egg or egg fractions. Weight and intake data provided the informa- tion needed to compare the rate of growth, maximum weight attained, and efficiency of food utilization. The influence of age and/or diet on the size of the organs was determined by weighing immediately, at the time of sacrifice, the liver, right kidney, right adrenal, and right lobe of the thyroid. Also, at the time of sacrifice, any gross abnor- malities in the animals were noted. The left kidney and adrenal, the left lobe of the thyroid, and part of the median lobe of the liver (approxi- mately 20 percent by weight of the total liver) were fixed in 10 percent formalin to preserve these tissues for histological examination. Other tissues prepared routinely for histological examination included the heart, lungs, aorta, salivary glands, spleen, pancreas, and testes. These tissues were embedded in paraffin, sectioned, and stained with hematoxylin and eosin. The methods used for rating the microscopic findings and the results of gross and microscopic examination of the tissues are being reported in detail elsewhere (54). This report includes only those phases of the histological findings that are related to the chemical investigations reported, and deals chiefly with the kidney and _ liver. Damage to the liver and kidney was rated arbi- trarily 1 to 4, with 4 the most extensive damage. Chemical analyses included determinations of moisture, fat, protein, and ash in livers and kid- neys, cholesterol and protein fractions in blood serum, and coagulable protein in urine. Most of the chemical analyses were obtained for rats that were fasted before sacrifice. Immediately after removal of the section of liver retained for histo- logical examination, the weight of the remaining portion was recorded and both the liver and kid- ney were stored frozen, for chemical analysis. Homogenates of these organs were prepared by use of a Virtis homogenizer, and the various analyses were carried out on weighed aliquots of these homogenates. The values reported for the liver content are for the whole liver, based on the assumption that the composition of the sections analyzed was similar to that of sections removed for histological examination. During the early series, small kidneys were pooled in order to obtain sufficient material for duplicate analysis. Once the conditions had been established for producing a uniform homogenate from the kidney, the results from chemical analyses showed excellent reproducibility. It then seemed preferable to analyze individual kidneys to permit a direct comparison of the results with the other measurements under consideration and to avoid masking differences that may result from average values. Only one analytical value could be obtained for kidneys weighing 2 grams or less. Duplicate values were obtained when sufficient material was available. The methods for analysis of tissues were the same as those used for the food samples, except that fat determinations were done by the AOAC acid hydrolysis method (13). The coagulable protein in the freshly collected urine samples was determined by precipitating with 5 percent acetic acid (63) and weighing the washed, dried precipi- tate. Blood serum samples collected at the time of sacrifice were stored, refrigerated, at 4° C. until analyzed for cholesterol and for the various protein components. Serum cholesterol was determined by the direct method of Zlatkis, Zak, and Boyle (192). It has been established (108) that the values obtained by the use of the direct method are high, but further investigations in this labora- tory have indicated that the relationships reported here are substantially correct. Serum protein components were determined by electrophoretic analysis, using the method of Tiselius (183) as modified by Longsworth and Jacobsen (118). Results and Discussion Composition of Experimental Diets On the basis of current information, the majority of the diets investigated provided adequate amounts of the nutrients essential for the growing rat and amounts generally considered adequate or more than adequate for maintenance of the adult animal. Admittedly, our knowledge of the re- quirements of the rat at various stages of life is far from complete. The analyzed or calculated values for the nutrient content of the diets are summarized in tables 2 through 7. Corresponding information for the ingredients used in preparing these diets is summarized in tables 75 through 78 of the appendix. To facilitate evaluation of the adequacy of the experimental diets under con- sideration, the amounts of nutrients suggested as requirements for the growing rat and for the adult rat have been included when such informa- tion was available. Many factors may influence the requirement for a specific nutrient such as the heredity of rats under investigation and the pro- portion of other dietary components. Most of the values for nutrient requirement that are re- corded represent the average of results obtained by several investigators, and have been limited to requirements of rats fed relatively simple diets similar to the semipurified diet used in the in- vestigations reported in this bulletin. Protein The protein content (table 2) of all but three of the experimental diets was within the range of 25 to 30 percent of the diet—an amount suggested for optimum growth of the rat. Two diets, SPM with 23.8 percent protein and SPW with 24.6 percent protein, were only slightly below the 25- percent level; the third diet, consisting of 100 percent whole egg, provided a considerable excess. The stock diet contained most of the essential amino acids in relatively small amounts when compared with the other diets under investigation but still provided sufficient amounts to meet requirements. The diet consisting of 100 percent ege with its high protein content supplied the essential amino acids in amounts considerably in excess of requirements. The other diets provided from two to four times the amount of the essential amino acids required for the growing rat, and still greater excesses for the adult animal. Considering tryptophan as the base line, no marked differences in the amino acid patterns of these diets were observed except for stock and SPW diets. The stock diet contained relatively small amounts of tryptophan when compared with the other amino acids. Methionine and cystine were high in SPW diet in relation to its tryptophan content. Fat Data for the fatty acid composition of the diets are presented in table 3 as a percentage of the dietary fat and in table 4 as a percentage of the diet. No data were available for the fatty acid composition of yeast fat, and the results recorded are exclusive of the fat from this source. The small amount of fat in the yeast (0.5 percent or less of the diet) would not be sufficient to change materially the major characteristics of the dietary fat. Table 3 also includes the iodine values for the dietary fats, and table 4 includes the choles- terol content of the diets. The iodine values of the food fats (table 3) ranged from 33.4 for SP 8 butter or SP 16 butter to 87.8 for SPPB diet, reflecting the relatively high linoleic acid content of peanut butter. Of the fat in SP 8 HVO, SP 16 HVO, SPa 16 HVO, SPb 8 HVO, SPEW, and SPW diets, more than 60 percent was oleic acid. Saturated fatty acids of chain length 16 or less accounted for more than 40 percent of the fat in the diets containing 8 and 16 percent butter. The concentration of fat in these diets (table 4) differed widely, with the smallest amount 6.3 percent in the stock diet and the largest amount 58 percent in the diet consisting of 100 percent egg yolk. Although the daily intake of the latter diet was less, fat consumption was higher on this diet than on any of the other experimental 3 “poqiodoi BVP ON 9 ‘lomnqovynuevur Aq pofddns son[va yotp YooIS = ("gL o[ vy x~puodde oag) ‘son[@aA pozA[Cuy 5 “(L9T-98T ‘dd ‘9) “stnburpy :001n0g ¢ (861) LOQOW ‘90.1n0g ¢ “SpOOJ [VNPLATIPUL ULOIJ PoPL[NO[VI SoON]VA “JoIp 3904S OJ ydoox gy 1 0 LG ise es CGM.» ee nea 08° 02° 83° $9" 83" ee | ates = |e? in ance noes nas |e nue oan ~“Wnpy L° are or Samer aa ey ia OT 8° ¢° g° z OG=OG a ae duno xX 1S] U0UL -dUINDOI POYVUALSHL OL'T 96° Go" bre €9'T OF" 86° 0€ 'T T OL'T oe 62° 80°T 9 'T T9'T £6" ca" SSG, eee a Gh Gee lero oP wr aes MOT £68 09% 99 °T 109 82 °8 CLT 20°€ 8P'E z OLS 60°T OL'T 00°E GL ¥ TE'é £8 °% LL* 8 OF ~~ ysl :S}OIP AOJ os UvYT 29% 4 ral b0'T $9 '¢ €9'T 29° G0°% £073 if 08 'T og * 9L° 96'T 6h % 18 'T 09 'T er" 9°66 =| 93'9 «| SLR PT ~~" 00TA £6 86'T 99°T 62 °9 82'S alt L0°€ 8h'E G OLS 60°T Lb'T 00°€ ama, IL'€ £83 LL” 8°OF | S6°O | GFL PO ~~" 00TH 68 'T 96° 00°T brs IL'Z 99° 68 'T 68 'T T QP 'T eo° OL'T £91 L0°% oo alt RE° 9 °bG “OAH 8 MdS 8L°T 181 89° GE 'b 66°T 99° Té'T PLT T GT 18" 19° 96 °T PFS 6¢ T $21 OF" 8°96 | 089 | GOR PTT ~""AAdS 16° CLS LL’ LE°9 99% 92° LE'T 80°% I 8o'T 8F° 88° St 68% L8°T 88 'T LY* Tos | 189 | 82° J "MAS 8L'T 09°% 29° 109 L9°% 68" ¥e'T G0'% I 09 'T OF" 18° 6h S OL's 16°1 eP'T OF" 9°66 | 889 | 89% J “OAH 8 4d8 8L'°T 09% 29" 109 29° a8" ¥e'T 60% I 09 'T OF" 18° 6h '% OLE 16'T eT OF’ 9°62 | 89 | 89°F | “OAH 91 ®d8 6P'T GL SG gg" o6 0 % 89° F0'T OL‘T T ¥e'T ee" 19° L0°% go % 8o'T 02 'T IF" $9 «=| 889 | 668 [OO ~~ dyn 9T dS 6h 'T GL % 99° G6'P G 89° POT OL'T I ¥e'T ee" 49° L0% Go'% 89'T 00 'T ia €9e «86| €8°9 «| 66° ~doyNG 8 ds 6F'T SL's gg 96°F % 89° b0'T OL'T I FT ee" 19° LOS Go '% 89 'T 02 'T ia be GRA ESA i Je PAB OL ds 6P'T SL % gg: 96°F % 89° b0'T OL'T T ¥G'T £e° 19° L0°% go'% 8o'T 02 °T Ir’ 9G =| 889 | 668 PU Pll 8 ds 6P'T CLG gg" 969 4 89° $0'T OL'T T aa | ee" 19° 10°% 99% 891 02 °T iz €9G | 889 | 668 fe “““OAHL 9T dS 69 'T 10% 82° eleg G 69° 8F'T 99 'T I 08 T 98° 69° 18° iad T9'T aa | 6S: 6°96 «6©| 819) | SLR OT a add 8 09°T 9L'% CLT brS 7% 16° Co 'T 16 'T T OFT a 8L° ca'% 98% 8L°T OFT th O°0g =| 089 | OLR [OT ~~ a dS ert 81% og" 96°F % 99° 86° 49 'T I 6I'T 08° £9° 96 °T bb G co 'T PLT 88° BES. VEO! GGA Go WW d8 OL'T 60°% £8" 91 '¢ % 62° go'T FG a! 19 'T Zo ° 18° 08% $6% 96°T 8h 09° 8°08 | 089 | eB pO Hd 8 6F'T a ard go°0 96h % 89° 60'T OL ‘T 161 bo'T ee" 19° L0°% co'% 8¢o'T 02 'T I 6°99 | 889 | 66° | “OATL 8 dS (9) (9) (9) (9) (9) OF 0 eh 08 'T (9) OL'T £10 620 80°T $91 99 'T £6°0 a) aL CN (SAR a © A i i a 90S SULDIP) | SULDIF) | SULDAZ) | SULDIY | SDL | SULDI | SULDID | SULDIY | SDI | SULDLID | SULDID | SDL | SULDLD | SULDLH | SULDLDH | SUDI | SDI | WL | SULDLYD SULDLE prow oULIOg jouTporg jouATH] ole, | prlovoy jouruvtpy} outp Oulu | OUNVA | oUIS | oUTURTeloUTJsAO]} oUTUO | OUISATT joUTONOT| OUTDO our | ueyd jutojorg |; aojory | , uod 4oldL ApH | -aedsy “suey | -18.0y -O1AK, |-[Auoy “TOTAL -N9OST | -Oo1qyL, |-OVdAAT, ULoJO1 | -O.UYIN wu ayy Lof ., spuamaunbas pasabins pup Yap fo swoub oop vad JUazywod , pap OULD PUD UWII}I0NJ—"Z LIAN J, “uIBIS GO’ UY} SsoT z “qBy ysvod JO DAISNIOXO o1B SONTeA DUTIPOT puB sploe 447R] {ysvod IOJ o[qRTIVAR BIVP ON (‘92 21G¥} xIPUOdde 99g) ‘SpOO} [VNPIAIPUL UI 4BJ JO WOTJISOduIOD UIOIJ PoPB[NI[VO SPOIp IOJ SONTBA _ sist ood xi COMMr~OKOARDH WoO HOWMAHHHROHOOO oO Pe Rarkarhenkond ys A>] +] wR 8°28 ony[Ba aurTpoy (z) ea Gee |r Se T° F 6g | oo 6 S| 6220s T° (z) (2) Ree Ie SECC i eee aaa MOT $3 FT ¢ to | y'S9 | GG O82 | LOS LPL | Sl0S) 1 62% 9°E Sia: 8° La 0'€ (Ohl (T = Ue ae ESOS Ys :SJOIp JOJ osuey G3 ian 8'2 Slr | ¢°¢ Lee Oae a es 6S He ize lege lee co oP © walle oe al wee NUS eee btn” “““O00TA G3 VT 82 8 27> SS ae |e 6 a 6°S atte ae Wie aiec ss aller. ei = eae eg OHS Gane eo ioe OOTH SKS Se (és Gad ¥99 | €° OSzZ, (9-8 €°9 OTE 9 Se Soen lpr le ces |ss = eae) IOEGG penises OAH Sods TG FT Leek e-lp | pS CaO | aes T 9 G VS | 9 ii (z) (2) lie {hy hoes are aor eet es ae AUdS ile Go QRZ 6°€9 | Fv’ 912) 9 T 9°9 9 FI | 6 (a i (z) T° | GAUGE | eae ae MudS (<) tia ve9) 9'19 | G° 0°69 | FT OL CGT) || tGeat T° oe la (Oe Ve OSOGa i tie week OAH 8 4d8 (z) G 69 PCO] F- 602 | ST 49 8F7L | OT Oh eae (z) les (a AVG |e nana a OAH 9T ®d8 Sp 8° 6G PSE | 0G TAGST |e oe oe 0 ‘ZGI | 8°S% | 6°L 9°E tae 8° ZI: ORS GOS | sae ae eas 1094nq 9T dS ca 8° 6G FSS. (3006 V i6€- io 2 eae 0O'CI | 8°92 | 672 9°E ET Sai Ley 0'¢ CrOGs |e 2 eae = 133404 8 d§ a 9 TOL | o°Sh | T° i aS ae pt We ¢ ‘0s | v° Gc" TE? () 1 Gc" GG Sale ae nes pre, 91 dS a 9° 8 6 9h | 3° 9 GG J 6°L €°0€ | 8 ian lee le (an ca ONOF) |Get i teens Soe PIeT 8 dS (z) (a 0°2 2°€9 |v €'1L | ST 9°9 Levit OT GS" ey (2) | Bae G- CAG: sa lee OAH 9T dS Tis Ee G ‘3S | OLY | 6° 8 69 | To 69 Oh Gl er Gaall G | te (z) T° i SNS Gate tee ogee addd$8 (z) ls Le | $09 4.o° bre | ¢° 2PM SSG aes Ti (2) () | T° [be GuGSr an ab Le adds (a 9° v PT 8 FF | PT y1¢ | 9° 0OOr | 2°1¢ | €°S € 7S 6° ¢° LT 0G GColValepe eae WdS GT OT VL €°Eo |) 9S 8 ‘99 | 9° €°9 £.'O6- |Z." IE (z) (z) T° ibe 8 Gu eos canes eee adds (2) oO 8°9 & 769 | § 0 2°69 | ST 69 G GE. | 26-0 FO T 0 TO (am) SO GES Gish cerns nna OAH 8 d$ SUDAE) SUD 14) UD LE) SUD LE) SUDLY) SUDLY SUDIYH SU DL) SUD LE) SUD LE) SUDLE) SUD LE) SWDLE) SUD ALE) SULD LE) SUDLE) er) 08) Id Le) 8Iry 1 @ ear®) ow 1#30.L Or) bl) re) se) ate) 1) 8 ie) 78) 1820, ITV UL -Id -ouoy, pd spioe £448} poyeinyesuy, sploe A448} poyeinyeg yof hapjarp fo anjoa aurpor pup , yarp fo suns QQ] 4ad yuajuoa prov hyyoy—'§ ATAV I, “WMBIS [Q'Q UB SSOT , (92 9148} xIpuodds 90g) ‘syusTpolSul ULOIJ PoJB[NITVD ¢ C92 9148} xtpuodde 00g) ‘syuoIpoisul AIvJoIP WO] POYE[No[BO SontBA—ysRoA WUOIJ SPIOw A44BJ JO QAISNTOXAT z “qey ysvod Sulpnyout ‘sonpea pozATeuy 0 () |20° |9Z° {26° |Z0° o¢’s (60° |o¢° |er‘t |So° |Z0° (s) () |30° (20° |I8‘T |e@°9 [U7TTTTTT MOT 082‘ (SPT 18° |@S°h I02°246 |61°€ |92°48 |88° irs [PO FT [GST |T9° |eo° WT’ (66° |eS° l69'ZT [OSS |777 77777 Ysiy ‘SZOIP IOJ oSUBY 082‘% ISh'T 18° |S °F |O@L°20 1618 = =J9L LE [7777 Gracin HORI ICG ee Lee alee lease Slee el ce 69: -21..|0 S89! (2-355 ee OOTA O88 ‘T 40'T |0o9° |€8'°S Th ‘Os’ |e‘ l08‘4e |--77 77 Glace CS Ole le S| eee | eles epee, irate GOLGI: eG ram |e enenen a teed ae OOTH Om lec ZO° {8g° |€z°¢ |Z0° wSeS: LIST, AOS: CmelGiely OGOhe Wiha plese, Fae een gee 1S) 21.9 Se Pie eazes OAH 8 Md§g 18 er’ GZ°|88T «(joe's 26° PSRs eee OLE |Ser It’ 120: () GSO ZO = VEO Gi pte eer sr ANds 6 ra €0° |OT ‘LE |90 OT 90° 8S ‘Il 83° POT joe's iT" |so° Zo" GP 1Z0rn “NCOs “WSs WGCOT. leap ee MWdS €1 (5) |€0 19° |o9’¢ {60 126°9 |Sl° 9° IPT |FT° [0° |Z0° () (20° |PO GPG FG OT OAH 8 4d$ SI () |€0° |ST'L |P8 ‘OT 120° GIGI |9S° = IPT TT «OSS @ JLT SCO 20° () |Z0° |€0 Coy |LLE | OAH 9F ®d8 8F GO° 4 en (6) A a a ggg) oT €0'% |98% [SET |T9° |eo° IHL’ (63° [2S° \6r'6 |T‘zL [77777 10nd OT dg 6% £0" 10 93° |26'6 0%" Oss [Po 20'T |66°% |OL° [aE ZT° {20° |ST 10° «|66'b | 6 [TTT royynq 8 dg 6% 10° Ol’ OL T I$9°2 [20° CSG) hecns, Of T [Sts {20 €0° |20° (G) |20° {0 699 ILA. ro Pll OT dg 02 FO" go" 6{248° 96S «/Z0* VS.Ves | es OL° (69°% |20° |FO° (y) )" 120i" 01S 9G 83 poG Sale =e PLBL 8 dg Or (5) {80° |ST‘T [SZ ‘OL |20° PO GT \SS° LTT ‘IT s«8h'@ IAT" O80 *a0° G) (20° [80° OL‘ JL 2ZE yo 77 OAH 91 d9 8 ZO * ZO" 3=6j98'€ 120°8 [0° 86 TT |88° |90'T ]1Z'%3 |1%° |go° |Z0° (He IO ICO \SRae “\Ge20, Vesa Sse ddds GP () |20° OL’ |ee'6 {0° 12°01 60° |SL°% |PR P [60° [20° () G) 120° (20° |9F°2 I@°6E [TTT TTT ads 9g €0° Ol’ |tl° |SS°L |ro- 99 ‘8 |OT 89'T |F9'€ [68° |6e° [St'O |s0'0 IST rE CVoL clo Ll i\petats 5: wo Wds Lb 920 = {LT 82 TL |Fo 6 109° 8¢ ‘TL |Or 60'T [6S °E ler Z0 ° () G) (20° (20° |86‘R fRoZt Jo tttttTtT Wds OL () |20°0 j09 0 |2¢°S |F0°0 |6t9 {et 0 {190 |SE't |2t-o io 0 (+) G) |20°0 |s0'0 joe's | 6 TTT OAHS8 d§8 tema ima Waa aie Sigg | nee nro (tee ae if weg | ee pale ge eee ad leer peg ae acell ere tee [ieee oe |r ee gee egg eer eg e'9 Toner ==" 904 “BIN SUDLE) | UDLE) | SWDL) | SUWDAD) | SUDAE) |SUDID) | WDIE) |SWDLY| SUDLY |SWDLD | WDA | UDI | WDA | UDI | Wd |supsy | sUDLyH ee 8Iry alr) 8Ir4y 0 a ®) e)—08) ww i ih. WV = 4 - | [Rqo7,.)) 2% Bry are) re) cate) fe) 8 9%) ve) [BIOL ¢ [O10 “UL -Iq -OuOJy 1 3BJ VIC -sojoug, TROL z Splow A44RBj poyeunyesuy z Sploe A44BJ poywainyeg jaup fo supib QO] sad yuazwod pouaysajoya pun ‘prop fiqof Qo g—p WAV J, diets. The total fat content of the diet was, of course, a determining factor in the total content of the individual fatty acids and was responsible for the large amounts of oleic and linoleic acid in the diets composed entirely of whole egg or egg yolk. Although the linoleic acid content of the egg-yolk diet was higher than that of the SPPB diet, the total consumption of this fatty acid when egg yolk was fed was about 60 percent of the consumption for SPPB diet. The latter diet supplied about eight times as much linoleic acid as did the diet containing a comparable level of fat, chiefly as butter fat. Linoleic acid, the only fatty acid so far shown to be essential for the rat, was present in all of the diets in amounts sufficient to supply the 25 mg. per day reported (122) necessary to prevent the development of skin lesions in the young rat and to permit growth to proceed at a normal rate. Linoleic acid as well as other fatty acids may play a role in other phases of lipid metabolism, but the importance of specific fatty acid patterns in the nutrition of the rat has not yet been established. The cholesterol content of the diets was rela- tively low, amounting to less than 50 mg. per 100 grams of diet except for those diets containing egg. The concentration of cholesterol was 478 mg. per 100 grams of SPE diet and was exceedingly high— 2,780 mg. per 100 grams—in the diet consisting of 100 percent egg yolk. Vitamins The data in table 5 summarizing the vitamin content of the diets and the requirements for the growing rat indicate that all of the vitamins known to be required by the rat were supplied in ample amounts. The high level of some of the B vitamins in the semipurified diet and its modi- fications was provided by the brewer’s yeast, which was present in all of these diets. Although the thiamine content of the stock diet and of the 100 percent whole-egg or egg-yolk diet was low in comparison with the other diets, it was sufficient to supply the amount of this vitamin considered essential. Choline requirements depend on many factors (85) such as sex, strain, age, and dietary methi- onine, cystine, betaine, or cholesterol. Although 10 to 20 mg. daily have been fed by many investi- gators to supply the needs of the actively growing rat, this amount is considerably more than is necessary under some circumstances. In diets containing from 24 to 30 percent casein, chloine requirements are small (0 to 6 mg. daily). The requirement of the rat over 30 days of age is also small. According to Slanetz (173) only from 1 to 3 mg. daily are required by older rats. Although the level of choline was low in most of the experi- mental diets reported in this publication, the amount supplied should be adequate for the adult rat and even for the growing rat in view of the methionine content of these diets. Levels con- siderably in excess of requirements were provided by the diets containing egg or egg yolk unless the high cholesterol content of these diets increased the need for choline. Vitamins A, D, and E were not incorporated into the diets, and the values recorded for these vitamins are in terms of daily intake. The chief source of vitamins A and D for all except rats fed the stock diet was the supplement of percomorph oil. Carotene from the kale supplement also con- tributed to the vitamin A potency of the diets. Egg and egg yolk were the only other foods to provide significant amounts of this vitamin. The supplement of dl-alpha-tocopherol acetate supplied generous amounts of vitamin E compared to reported requirements. Nerurkar and Sahasrabudhe (137) reported that pure vitamin A is toxic to young male rats when given orally at a dose of 40,000 I.U. daily. When feeding was continued for 10 days, there was a gradual decrease in the percentage retention of calcium, phosphorus, and nitrogen. The toxic dose of vitamin D is generally high, but no exact data can be given. In man and dogs (161, p. 430), 20,000 I.U. daily may produce toxic symptoms. The amounts of vitamins A and D in the experi- mental diets under consideration in this publication were in excess of requirements, but they were considerably below the amounts that have been reported to be toxic for relatively short-term studies. Minerals The salt mixture used for preparing the semi- purified diet was the chief source of minerals and, as seen in table 6, relatively small differences were observed in the ash content of these diets. The stock diet contained from two and a half to three times the ash content of the experimental diets, and large amounts of calcium and phosphorus in satisfactory proportions. Diets consisting of 100 percent egg or egg yolk were low in calcium, with a ratio of calcium to phosphorus below the desirable range. Potassium intakes were consider- ably in excess of requirements. Manganese tended to be low. Aluminum values have not been included. The were high and variable be- cause of comtamination with aluminum from the erinders used in preparing these diets. Calories The data for the calorie values of the experi- mental diets are summarized in table 7. In the majority of the diets, sucrose was the chief carbo- hydrate. The cereal starches supplied most of the carbohydrate in the stock diet. Fat supplied less than 20 percent of the gross calories from the stock diet and from the various modifications of the semipurified diet containing approximately 9 percent of fat. The remaining diets, except for E100 and Y100, supplied 30 to 35 percent of the calories as fat. The calories from fat in diet Y100 were 74 percent of the total gross calories from this diet. (66 “4 “@e7) AOD :oo1n0g = *oywyUT UIvAS-QT JO SISUq UO “YT OF 10 ‘pooj Jo Wes aod ‘AI € JO UOTyIppe Aq poaoad “WI ST qolp ‘osuvd sty opisyng “yuosoad st snaoydsoyd quooaod ¢'Q puv ‘fz puv [:] UdoMyod SI yoIp oYyy UL OTyUA snaoydsoyd-wntoywo jl paatnbod YON} “(8LP “d ‘797) Sanquosoyy :o01M0g g "p 9JOUYOO} OVG = “Yolp OY} UT ST UTosvo UOYA [eIyUOSSe JON , ‘p 9JOUYOO} OSTV 90g ‘ose pu “UIRAYS ‘xos SuIpNpoul ‘s1ojovy AULA UO YUopUDdop syuoWoIMboy °z OYOUYJOOJ DOG ‘SAOATT AYJVJ PUG SUOISOT [VUOI JUDADIG 04 JUDLOTYNE 4 ‘syed poyo[dop-ey ULHULYTA JO 0FRA YIMOIS YOoM-F UO posuq JoIp JO SURAT YO] 10d “Sr T YIM wUNWIXeUL 04 OSOPD YYMOIS Sulyworputr wyep Wor “pL “OASIog ‘OolAdog youvosoy [eang[Nosy ‘UOISTAIGE Youvosoy AIpueqsnyy PeuUTUy ‘UvlaqQIv YT “JY INYAy YM VOTYworuNWIWOD [CUOSIOG ¢ “(¢E) JUBADJINGG puv UMOIG :od1IN0g “A[Iep swILAS QT JO UOTYduINSUOD UO poseg , “(@B1) AODOVJT 2091N0g ¢ 069 Osud ‘TF o[qey Ul VYEp WOAT poyL[NoTvo “(Sf) Toned, :00an0g “Avp dod ‘sur | Apoywwurxoidde poynqiayuoo 4044nq ynuvod Sururezuoo sylqE *yuoor0d QT SUM [OAR] UOYM “HUT gE IO “RJ SIYY JO yuooIOd g SUTUILIUOD SJoIp UL OAH Aq poynqiayuoo Avp aod “Sur g*y AToywurrxoadde ‘uory[ppt uy “quouroyddns sv pogp ; ‘lounjovjnuvw Aq pol[ddns yolp yoo4s toy vyeqE “22, o[qv4 xIpuoddy oog —“syuotpoasul woay poyepnopeo o1aM SonTeA “JoIp YOo4s oJ ydooxny y OTe O€-OT 8 OF ‘¢ GST + (ear O€ 6 Vy O. O€-02 9 OTe O'Ts OOT re Joo 0+ “GZ s OOM ¢. |p otn esse 2 tae pel SUNOX ‘SPU WIN DIL poyeulysny Tg COR 006 F'8 GG G8 LL° io) a 10° Or - 99 ° SG” OP een MOTT T'¢ 9¢ 090 ‘T 0 “St OFL ‘ZS LT 66 °L ST T TS ° 96 °L 61 T EOD Ee UST] ‘SPOIP LOF OSUBYT 1g 9g 0f0‘T | 0 FF OL ‘SZ Ll | 26°. Sit 60° OL’ 99° ST et eee ne oe oes OOTA Tg 9S OS6 0 SP P98 ‘T 0 OL PEL PG ° TO" 0Z ° 90 'T VG ele ee a ea ees OOTEL Lg 9G O19 8 ‘61 OG) ia ee GST GG * TS ° LI ¢ 6T T O0K98 Tae oe OAH 8 Md§ T‘¢ 9g O18 6 ‘8ST 6E8 IT ¢ 13 '€ OS * T° LEE ay ia Vila: D lncaw. hs Ss oo AUds Leg 9¢ O19 GGL GG 06° 61 T 6L° ST° 96 'T 6° TASS "eae a ee ea se MiUdS T'S 9¢ O19 4°8 9G GE UT ee T GG 13° ZO “G 68° LOO ie eel bee oe mraae OAH 8 dd8 T'S 9¢ O19 1°83 9G GET Se ‘1 GG 13° ZO “G 68° OOD” | PS Bes Ses rs OAH 9T ®dS Eg 9¢ 069 9°8 9G 90 'T oe T GG 13° OG Gg ° TOMO”, il ie aime cea eee 10990q OT dS jie 9S 0S9 9°8 9% 90 'T Ge OT GS 13° 60 °G G8 ° LOEOe eS Sage ines ee 199ING § dS Ls 9G O19 9°8 9% 90 ‘T Goa GG” 13° 20'S G3° ROMO ree ne ae ee Plvy OT dg T'¢ 9S O19 9°8 9% 90 T Gs UT (a 13° ZO “G G8 ° TOg0) Biles? he sac ~ ag oo ares PBT 8 dS fea: 9G O19 9°8 9G 90 'T GET GG 1G ° GO 'G Gg TOO UN Ho ge oa OAH 91 dS T¢ 9¢ O19 6 PI OP G8 6F T GS * roe 96 “2 hee Voie Wer oo gg ee ee dddS tg 9¢ O19 v8 (Ay) 06° GST 6E ° 9T° 09 °¢ a Wao Glee oces 30 a ee adds T'¢ 9G 099 LTT OF 9¢ ‘T 6F 'T GG 9T° G6 E 6 Oa, wives. we ge, ee ee INdS T'S 9¢ OSL LOR OSPF O€ € GS GS O€ OT ° G8 ES 06 OORT ei ee a ere tldS Tg 9¢ O19 9°8 9% 90 'T GE CT GG 1G O'S G8 Os Vi ee wie a OAH 8 d§8 aa we Cex 006 0 ‘ST Tél Gh ZG Le0 83 ‘0 LO 96 °E 90 ‘T ad: ec ape ign @ ae ge IOS OW eae | at “bn ‘OW ‘bn OW 5 OW “OW OW “ON plow 2 OL ral Vv ulyorg | ourpoyp ley oruoyy oUuIxXOp poe UlDBIN, UAB]. OUTWIT, UlUUBITA | ULUUBITA | UTIL A UIWIBITA | -OJUBT Whig DIJO, -Oqnyy PIC ayByul ATIVE Jolp JO SUIBIS YOT Jod yuoquog JDL OY} LOf syuamawnbas paysabins pun ,‘syap fo yuayuoa UUDLA—'G ATAV Ada 10 TABLE 6.—Ash and mineral content per 100 grams of diet, and suggested requirements for the rat Ratio— Diet Ash! }Caleium| Phos- | Iron | Copper | Sodium | Potas- Mag- Man- |Boron|Calcium: phorus sium nesium | ganese hos- phorus Grams| Grams | Grams | Mg. Mg. Grams | Grams q. q. Mg. Stockiweesn see 2s 0. 0 2.17 1, 22 | 17 0. 7 0. 64 0. 59 290 3.20 eels 1.8 SPs) H VO 82.2 eS 3. 6 . 56 . 52 | 14 5 . 16 . 78 83 . 66 0. 14 ial Fel Go) the eee ie ans 3.1 . 41 .o1 | 14 .9 . 22 . 68 72 solo. 13 .8 SIR Nisa tee Pi 3.9 . 58 .42 | 12 .8 Ss . 81 57 .46 | .12 1.4 SR Bsteet nese ole 3. 1 . 36 .45 | 12 1.1 13 aie 67 .45 | .17 .8 SREB ems st ake 3.1 . 46 .48 | 14 1.0 15 . 95 110 1.50 | . 32 10 SP 16 HVO 4_______ 3. 6 . 56 .52 | 14 1.5 16 . 78 83 . 66 | . 14 1.1 SiRSilardst2 2-3-2. 22 = 3. 6 . 56 . 52 | 14 1.5 16 . 78 83 . 66 | . 14 1. i SP 16 lard 4_________ 3. 6 . 56 .52 | 14. 1.5 . 16 . 78 83 66 | . 14 ieee SP 8 butter 4________ 3. 6 . 56 .52 | 14 1.5 . 16 . 78 83 66 | .14 eet SP 16 butter 4_______ 3. 6 . 56 .52 | 14 1.5 . 16 . 78 83 66 | . 14 ial SPa 16 HVO 4______- 4.1 . 54 .51 | 13 .9 . 16 . 98 85 . 46 | . 06 ial SPb 8 HVOt_______- 4.1 . 54 . 51 | 13 9 . 16 . 98 85 . 46 | . 06 11 IPE Wie tees re ae 3. 3 47 .43 | 11 a7, . 22 “93 82 . 40 | . 06 11 SBHYatee se See eS 3. 3 . 43 .54 | 11 .6 14 . 69 61 . 34 | . 06 .8 SPW 8 HVOt________ 4,4 . O4 .44 | 12 nat 35 1.18 104 45 03 1,2 LOO 3 eo ee 3.5 .21 . 62 8 .4 . 40 50 42 .18 aL 7¢ .3 WT OO Goce Biri eb et 2 3. 2 . 24 . 72 8 2 11 13 16 13 10 an) Range for diets: Migherss. =) 2! 10.0 2.17 2A ay, 1.5 . 64 1.18 290 3.20 | . 32 1.8 Wows eee 3.1 .21 . 42 8 .2 ail 13 16 Beales | Pes} a3 Estimated require- ments:° Young ratevso |b 2 . 50-. 60 |. 45-. 55 2.5] .5-1.0 . 07 ~ 15 Dili ero . 008 | 1:1-2:1 1 Analyzed values. 2 Except for ash values, data supplied by manufacturer. 3 Analyzed spectrochemically by A. W. Specht and J. W. Resnicky, Soil and Water Conservation Research Division, Agricultural Research Service, Beltsville, Md. TasLE 7.—Calorie values per 100 grams of diet, and percentage of calories as carbohydrate, fat, and protein Percentage of gross Heat of calories as— Diet com- bustion Carbo- | Fat | Protein hydrates Calories SHO 6) feats ay St aa 1399 AT 14 39 SIRES EIA, © ew ie eet 1470 50 19 31 ft Bl ab sceet etl Rig tn sc 1520 35 32 33 RO PANY Legieteee ok aS. ee ee 1493 41 32 27 SIRI ee pa eee at 1520 33 35 32 SIP IRIB Bese pr cere yn (2 1498 39 33 28 SP 16 HVO2 220222. 1504 40 32 28 SP 8 lard_____ sue AR al 1470 50 19 31 SP ligulardias 2s 1503 40 32 28 SP Sbuttersea 22 470 50 19 31 SP 16 butter_______- 503 40 32 28 SPa 16 sHVOe 222-2 506 35 31 33 SPb CO VOn. ee 468 44 19 36 SIR Wise aa cane 2 511 37 30 33 SIRs Pg 523 38 33 29 SPW 8 HVO_______- 460 52 18 30 OOS Seas ae 684 2 59 39 Ys TO Ochs et ca 739 3 74 23 Range for diets: Wen spl oe ae re 739 52 74 39 E70 i ee ay re 399 2 14 23 1 Analyzed values; all other values were calculated by using 5.65 Calories per gram for protein, 4.00 Calories per gram for carbohydrates (chiefly sucrose), and 9.3 Calories per gram for fat. 4 Calculated from ingredients. See appendix table 78. 5 Source: McCoy (122). 6 Minimal requirements for reproduction. Body Weight and Longevity as Influenced by Diet Body weight, intake, and age (stock, SP 8 HVO, and SPE diets) WEIGHT GAIN IN RELATION TO FOOD INTAKE (SP 8 HVO anv SPE prers).—Records of food intake and weight throughout life were maintained for 44 rats fed SP 8 HVO diet and for 38 rats fed SPE diet, and included 4 separate experimental groups of animals allocated for longevity studies on each of these diets. Data for individual groups as well as average values for all animals are sum- marized in appendix tables 79 and 80. Figure 1 represents graphically the average food intake and gain in weight of these rats from weaning until 300 days of age, a period relatively uncomplicated by the occurrence of excessive weight loss or death. The general pattern of food intake and weight gain was similar for both diets, although young rats fed SP 8 HVO diet tended to gain more slowly than did those fed SPE diet. From the second to the sixth week on the experimental diets, the animals progressively increased their food intake and maintained a relatively constant average rate of gain of approximately 40 grams weekly on SP 8 HVO diet and of 45 grams on SPE diet. The rats were still continuing to gain weight at 300 days of age, although their intake was relatively constant after the sixth week. i1 WEIGHT GAIN (Grams per week) SP 8 HVO 0 40 80 120 DIET FOOD INTAKE (Grams per week) 120 FOOD WEIGHT 80 INTAKE GAIN oA s—A SPE o--0 40 0 160 200 DAYS ON DIET Figure 1. Average weight gain and food intake of rats fed SP 8 HVO and SPE diets. Bopy WEIGHT AND AGE—INDIVIDUAL RATS FED SP 8 HVO anv SPE prsets.—Many of the rats continued to gain as long as they appeared to remain healthy, increasing their food intake when- ever there was a tendency for their body weight to remain constant. This response is best illus- trated in figure 2, which shows the change of weight with age of three individual animals. Curve | represents data for a rat that was fed SPE diet and died before he reached 400 days of age. This animal gained rapidly for 350 days, at which time a precipitous weight loss occurred in spite of a food intake averaging 17 grams per day. Curve 2 shows the continued gain for 550 days of a rat fed SPE diet and the rapid loss in weight that occurred during the 25 days before death. This animal had decreased his food intake slightly from 14 to 12 grams daily. Curve 3 represents the body weight of a rat that was fed SP 8 HVO diet and was still gaining when 800 days old. A marked decrease in food intake from 19 to 9 grams daily paralleled the decrease in body weight ob- served between 800 and 900 days of age, at which time the animal was obviously moribund. AVERAGE BODY WEIGHT AND AGE (sTocK, SP 8 HVO, anv SPE piers).—Data for weight changes throughout life were obtained for 53 rats fed SP 8 HVO diet and for 74 fed SPE diet. The tendency for continuing increase in weight with age noted for individual rats (fig. 2) was not apparent in the average weight curves of rats fed these two diets (fig. 3). The third curve in figure 3 represents cross-sectional data available for a large group of rats from the stock colony that were sacrificed at different age intervals. Animals fed the stock 12 diet tended to be smaller than those fed SP 8 HVO or SPE diets and showed little change in average weight after 250 days. Of these animals 52 per- cent weighed between 450 and 500 grams; 2 per- cent exceeded 600 grams. In contrast, 59 percent of the rats fed SP 8 HVO diet and 61 percent of the SPE-fed rats weighed 600 grams or more, and 21 and 18 percent, respectively, exceeded 700 grams in weight. Rats fed SPE diet appeared to have reached their maximum weight by 350 days, whereas those fed SP 8 HVO diet attained a comparable average weight between 500 and 600 days of age. The lower average weights observed for the older surviving rats fed SP 8 HVO and SPE diets seem to be due to the early death of many of the heavy rats, and will be discussed further in relation to the longevity data. Discussrion.—Reports in the literature on weight changes in the rat throughout life have been chiefly for diets of natural foods suitable for raising stock animals. Changes made in the diets of stock animals based on increasing knowledge of their nutritional requirements have resulted in an appreciable increase in their size. Mendel and Hubbell (128) have reported a gradual increase in rate of growth of their stock (‘“‘Yale”’ strain) rats over a period of 25 years, with the most marked change occurring when the diet of Anderson and Smith (9) was introduced. This diet produced adult animals weighing about twice as much as rats on the earlier stock diets. This change was attributed to diet rather than to selective breeding, and the improved growth rate was accompanied by superior reproductive performance. Mayer (127), also using the ‘‘Yale” strain of rats, reported GRAMS 600 200 400 #]. a----o SPE #2. a---a SPE #3. o——o SP 8 HVO 600 DAYS 800 1,000 Figure 2.—Weight in relation to age of selected individual rats fed SP 8 HVO and SPE diets. still more rapid weight gains for animals fed a synthetic diet. Inherent differences in the growth potential of different strains of rats complicate comparison of the size of animals from different laboratories. Mature animals from the stock colony maintained in this laboratory appear to weigh as much as or more than most stock rats of comparable age from other laboratories. Their average maximum weight was slightly less than the 522 grams re- ported for the rapid-growth-producing diet of Anderson and Smith (9). Rats fed the semipuri- fied diet reached weights comparable to those observed by Mayer (127) using a synthetic diet. Numerous equations have been suggested to represent changes of weight with age, and several investigators (44, 53, 78) provided evidence for the usefulness of the equation proposed by Zucker, Hall, Young, and Zucker (/94) for evaluating rat growth and relative efficiency of various experi- mental diets. These authors proposed an empiri- cal formula for expressing growth which defines K, a growth intensity factor, and A, an inherent size factor. When the formula was applied to data from their laboratories as well as to data from other laboratories, these authors report that a straight line was generally observed and_ that neither size nor growth rate appeared to affect the growth property measured by K, the slope of the line, as long as the diets were free from growth- inhibiting factors. Dunn, Murphy, and Rock- land (53), however, observed a change in the GRAMS 600 400 CROSS-SECTIONAL DATA** LONGITUDINAL DATA * a—a~ SP 8 HVO o----0 SPE o—ao Stock 400 DAYS % NUMBER OF RATS SURVIVING; ANIMALS INCLUDED AS LONG AS HEALTHY. REACH POINT REPRESENTS DATA FOR AT LEAST 35 ANIMALS, Ficure 3.—Average weight in relation to age of rats fed stock, SP 8 HVO, and SPE diets. 13 slope of the line at about 14 weeks of age for rats fed the rapid-growth-producing Anderson-Smith diet, and suggested that this deflection may be related to the beginning of a normal adult period. Copping, Crowe, and Pond (44) observed a deflec- tion at 11 weeks on two of the eight diets that they investigated, and suggested that this deflec- tion may be due to the rapid early growth of rats on these two diets that contained more than ade- quate amounts of protein. When Zucker’s formula was applied to the data here reported for rats on SP 8 HVO and SPE diets, a straight-line relationship was found to hold reasonably well until the rats reached 15 weeks of age. During this period no deflection was apparent that would indicate any nutritional deficiencies. The K values of 3.8 for SP 8 HVO and of 4.0 for SPE diet were similar to the 3.8 value reported by Zucker for male rats. The change in the slope of the line after 15 weeks provided further evidence that the age range over which this formula applies may be limited when very rapid growth occurs in the young rat. Everitt (58) and Berg and Harmison (20), reporting data relating body weight to age throughout life, observed a rapid period of growth followed by a plateau similar to the results re- ported here (fig. 3) for rats fed SP 8 HVO and SPE diets. The subsequent decline in body weight which these authors observed was also noted for the majority of rats fed SP 8 HVO and SPE diets, as seen in figure 2 in the examples for individual rats. Although there is general agree- ment that such weight loss frequently occurs before death, there still seems to be some question as to whether or not weight loss is a necessary accompaniment of the aging processes. Everitt (58) reported an average decrease in weight from 381 to 249 grams during the last 200 days of life of 68 male rats. Eighty percent of the animals had lung abscesses, but their loss in weight was similar to that in a comparable number of rats with healthy lungs. No data were reported on the incidence of other lesions except for three tumors. According to Everitt and Webb (59), this weight loss may be due to disease or to senescence. Results obtained with BHE rats in this labora- tory, however, as well as those reported by Berg and Harmison (20), indicate that weight loss in older animals generally reflects some pathological condition. These authors separated the results for rats with no lesions from those with lesions. The average age of the group without lesions was 681 days. The body weight of these rats increased with age up to 522 days, with no marked difference thereafter. A progressive decrease in weight with age was observed in the older rats with lesions. Growth and food intake of young rats (all diets) A pattern of weekly weight gain and food intake similar to that discussed for rats fed SP 8 HVO and SPE diets was observed for rats on the other 14 dietary regimens under investigation. Data for a minimum of 10 rats were generally obtained with each of the experimental diets. Although there was considerable variation in the response of individual rats to any one diet, the data reported for SP 8 HVO and SPE diets summarized in appendix tables 79 and 80 indicate that average values for groups of approximately 10 rats generally agree well with those obtained for the larger number of animals. In table 8 are summarized data on the weight gains during the first 12 weeks on each of the experimental diets, and except where indicated, the data reported have been confined to groups of littermates. Included also are data obtained on food intake in grams and in calories and on the efficiency with which these diets were used as measured by the calories-per-gram gain. Calories are reported as gross calories throughout this publication. To determine available calories from these diets, data are needed on the digestibility by the rat of the various diets under investigation, on the energy from protein that is stored in the body of the growing animal, and on the loss of energy in the urine either as protein or as incom- pletely oxidized products from protein. The factors 4, 9, 4, frequently used for calculating available energy from dietary protein, fat, and carbohydrate, were developed for use with man from the extensive investigations of Atwater and Bryant (14). Metta and Mitchell (131) showed that these factors are not applicable to the rat. MopIFICATIONS OF SEMIPURIFIED DIET—WITH SELECTED FooDS.—Young rats grew well on all of the experimental diets. The most rapid growth was observed with animals fed the diets in which ego, milk, beef, or peanut butter replaced 20 to 25 percent of the semipurified diet. Weight gains were similar for all of the diets containing these foods and were consistently greater than were those observed on the semipurified diet. The difference between 16.8 Calories-per-gram gain on SP 8 HVO diet and 15.3 on SPE diet was highly significant (P <0.01). Differences in digestibility of these two diets do not explain the differences observed in food utilization. Marshall and Hilde- brand (/25) recently reported that BHE rats were able to digest SP 8 HVO and SPE diets equally well. No significant differences were noted in the utilization of the diets containing ego, milk, beef, or peanut butter. SPE DIET WITH PURIFIED NUTRIENTS.—No change in rate of growth or in calories-per-gram gain was observed as the result of adding the various nutrient supplements to SPE diet. KIND AND LEVEL OF FAT.—Data for the group of diets containing different kinds and levels of fat were limited, and the calorie values of the diets were less accurately established than those for the semipurified diet or for the SP diets containing egg, milk, beef, or peanut butter. There was a consistent trend for rats fed diets containing 8 percent fat to grow more slowly than those fed the diets containing the same fat at the 16-percent level. Rats fed the diet containing butter tended to be smaller than those fed comparable levels of HVO or lard. The diet containing 16 percent lard was the only one used with an efficiency comparable to that seen for the diets containing ego, milk, beef, or peanut butter. The range of values observed was wide, and more data on this group of diets are needed to establish the significance of these trends. LEVEL OF PROTEIN AND FaAtT.—When diets contained 30 percent protein as casein and lactalbumin (SPb 8 HVO and SPa 16 HVO), erowth rate and calorie-per-gram gain were similar to the results obtained for comparable levels of fat when the diet contained 25 percent protein (SP 8 HVO and SP 16 HVO). EGG AND EGG COMPONENTS.—Rats fed a diet (SPEW) in which egg white replaced 30 percent of the protein in SPa 16 HVO diet tended to be smaller. Growth response and utilization of diets in which egg yolk replaced approximately 50 per- cent of the protein and approximately 80 percent of the fat in SP 16 HVO were similar to the results for rats on SPE diet. Rats fed a diet (SPW) in which egg white replaced all of the casein and lactalbumin in the semipurified diet were small. No data were obtained on the calories-per-gram gain for this diet. Evaluation of growth on and utilization of diets of 100 percent whole egg or egg yolk was complicated by the tendency to frequent diarrhea in the rats fed these diets. Food consumption and caloric intake of these two high-fat diets were low, and rate of growth was correspondingly reduced. WISTAR RATS FED SP 8 HVO anv SPE piEets.— Wistar rats fed SP 8 HVO and SPE diets ate less and grew at a slower rate than did BHE rats fed comparable diets. Wistar rats seemed to use both diets more efficiently for growth than did comparable BHE rats, but the differences ob- served may be due in whole or in part to the smaller weight that was being maintained by the Wistar rats during this period of rapid growth. However, both Wistar and BHE rats tended to grow more rapidly and to use their food more efficiently when fed SPE diet than when fed SP 8 HVO diet. Discusston.—Although the levels of fat in the diets investigated were not considered excessive, there appeared to be a consistent trend for rats to become larger at an earlier age when the fat level was 17 to 19 percent than when it was 9 percent or less. In some instances, the increased growth rate appeared to be explained by differences in intake alone; in others, a decrease in calories-per- gram gain—that is, more efficient utilization of the diet—accompanied the increased growth rate observed. Deuel (48) in reviewing the subject of dietary fat and growth, concluded that the preponderance of evidence favors the hypothesis that in the rat greater increases in weight and improved effi- ciencies of the diet are associated with increased consumption of fat. In studying the associative dynamic effects of protein, carbohydrate, and fat, Forbes and Swift (64) showed that fat is particu- larly effective in reducing the specific dynamic effects of diets. Reports in the literature on the response of the rat to fat have dealt chiefly with the influence of different kinds and levels of fat rather than with fat-containing foods such as egg, milk, beef, or peanut butter. Lard has been most frequently used in investigations dealing with the response to dietary fat. In weanling rats fed for a period of from 8 to 10 weeks, there has been a tendency for weight gain to correlate with the level of this fat. The differences reported have usually been small and in some investigations have not proved statistically significant. Hoagland and Snider (93) found weight gains per 100 Calories to be approximately the same for diets containing 15, 30, or 54 percent lard, but at these levels the gains were significantly greater than those observed with 5 percent of this fat. Hoagland, Snider, and Swift (94) found that the differences were not significant when isocaloric amounts of diets containing 5, 10.98, or 18.27 per- cent lard were fed. Forbes, Swift, Elhot, and James (65) reported weight gains to correlate with level of fat when rats were fed isocaloric quantities of diets containing 2, 5, 10, or 30 percent fat. Two percent corn oil was present in all of the diets, and the additional fat was lard. The largest difference observed was that between the diets containing 2 percent fat and those containing 5 percent fat. Forbes, Swift, James, and others (67) reported a similar experiment using large increases in the intake of 10 of the vitamins, and again demonstrated that fat confers efficiency of utilization of food energy for the growing albino rat; however, the small increments in weight gain observed as the level of fat increased from 2 to 30 percent were not statistically significant. Hoagland and Snider (93) compared the relative efficiency of lard and hydrogenated cottonseed oil as dietary fats. At all except the lowest level tested (5 percent), lard proved significantly more efficient in promoting growth than did hydroge- nated cottonseed oil. The diet containing 15 per- cent hydrogenated cottonseed oil appeared some- what more efficient than the diet containing 5 per- cent of this fat; at still higher levels the response was similar to that observed with diets containing 15 percent fat. Marshall, Hildebrand, Dupont, and Womack (126) fed ad libitum diets containing 15 percent hydrogenated vegetable oil or lard. Weight gain per 100 Calories during the period of rapid growth was slightly more on the diet con- taining lard, but the differences were small and not statistically significant. According to Barki, Collins, Elvehjem, and Hart (17), conclusions with regard to the growth-pro- moting properties of fats may be misleading if comparisons are confined to one level of fat. These 15 j Or~ndao Oe roid © mereid xs NO hwOMO10 80 ‘61-0 ‘ST | 2°91 OzE ‘2-O9T ‘¢ | 026 ‘SG | 099 ‘T-OOT ‘T | G2z‘T | Sgh—-G6z | 8gE 09-68 | LF Ole + ipa Ne gaaes caer OAH 8 4d8 4I-Z] ‘ST | 6 ST OLE ‘2-082 ‘S | OFT ‘9 | OFF ‘T-OGO‘T | OTZ‘T | GLP-FZE | S8E 9G-IPF | 9F OR J Secsore 2 eee OAH 91I %d8 ‘61-24 ‘ST | 6 ‘OT 0&2 ‘9-020 “F | 0&8 ‘G O€F ‘I-080‘T | OFZ ‘T 96E-E0E | SFE 09-68 OF Ol vs 92") =o rene OAH 8 dS —poj So} euLIoyyvyT ‘81-0 ‘91 | O'LT Og ‘2-000 ‘S | 080 ‘9 | OSF ‘T-000 ‘T | 00Z‘T | L2h-G8z% | FSE IG-88 | 2P (One se bee eee zoynq 9T dS Sl=G Ol wea OLO ‘2-082 ‘G | O88 ‘G O6F ‘I-OTT ‘T | 0&2 ‘T P6E-68S | LEE CS-8E PP OL at eo era 1099NG § dS OTe Si Oe ST 000 ‘2-001 ‘¢ | 068 ‘G 068 ‘IT-O10 ‘T | o21 ‘T LGV-8EE | LLE PS-6E $V (1 Ss gee ll Sie nen cml piv] 9T dS ‘8T-9 “GIT | 9 OT 092 ‘L-0F6 ‘F | 086 ‘G OFS ‘T-OG0 ‘T | 092 ‘T 61P-90E | OSE 8P-8E cP Oly . it ae ee ane ee ee ple, § dS il—-OmG ia seob 029 ‘9-0&T ‘G | O10 ‘9 OTS ‘T-0z0 ‘T | S61 ‘I TZP-GOE | OLE OS-8$ PV Ollie el 1k iene rane OAH 91 dS ‘21-0 91 | 2 9T O€¢ ‘9-028 ‘F | OT8 ‘G 068 ‘I-OFO ‘T | OFZ ‘I LOP-ELS’ | OGE OS-8E PP Ci Sith sees ce ee OAH 8 dS —povj $0} BULI04 VT aaa — = a —= — SSP-IPE | OOF Gcg-LEé LV ()) Ot eee | aE tee Ne ag % OF) ‘[O10}4 -sopoyo + %Z'0 ‘plow’ oIqIoosy — == = aa a aan OLP-¥CE | LOD Co-SE LY OR! ‘ales se ee: %S'O ‘plows o1q1oosy = =— a aa == = GhP-OTE | TOP PS-LE LP OW, J Res Sen) WET “Jor19ysopoy — = = = — _ 61G-16z | SOF 09-28 | SP OL —' |e er. %OP'O ‘o19ysoToyD, = —e = a = ce OSP-IEE | S6E 9S-6E LP Oi i/o era ae ae i Se ouoN —yYIM pejusuelddns qqs —po9j soyvur4e} qr] ‘OT=G FL | SST 098 ‘9-014 ‘G | 092 ‘9 0ZE ‘T-020 ‘T | 002 ‘T O9P-TEE | SOF I9-8€ SP Ole. ult ire re SS sees See ws OOT/ sur 100 “E+ "us OOT /su gO “Ga+%G'o ‘ourfoypD ‘OI-G ‘FI | € ST 002 ‘2-OT2 ‘E | OZT ‘9 OSs ‘T-OOT ‘T | OST ‘T L6b-SGE | 66E 69-LE SP OV, | et oe ws OOT fs oo “gq+%s'o ‘oulfoyg ‘QI-G ‘GI | 3ST 000 ‘2-0FZ ‘¢ | OLT ‘9 ocs ‘T-O10 ‘T | O6T ‘T TCF-G9OE | SOF T9-8€ OF Ol "Weaver es ws QOT/ su GO “_ Gi=p SL, (| 1 Sia OSF ‘9-OTG ‘¢ | OST ‘9 OFZ ‘T-090 ‘T | OST ‘T EFP-F9E | SOF G9-GP 6P Ole Vike sote oS ae MEO ‘ourpoyg ‘OI-G ‘FI | $ ‘ST OZT ‘2-002 ‘G | O86 ‘E OL€ ‘I-000 ‘T | OST ‘T FSP-8SE | OSE I9-LE SP Ole i ae oe ae ae 9u0 NT —Y IM poyusweddns WdS —pery $O}PBULIO} IVT ‘OI-G ‘PI | 2°ST 089 ‘2-069 ‘G | OG ‘9 | OSPF ‘T-OOT ‘T | 092 ‘T €IG-19E | 6ZPF Lo-GE | SP Ollie 0 |) 22 ete ne ws OOT jsut 100 “gq +% "0 ‘ourjoypD O-8 "St | bk St Oss ‘2-086 ‘S | OOF ‘9 OTF ‘I-OFT ‘T | OFZ ‘T P8P-68E | 8ZP 09-OP cP ORD Ales 2 ws QOT/sut TOO “ef ‘GI-9 ‘SI | 6 FT 089 ‘8-029 ‘G | 009 ‘9 O6F ‘IT-060 ‘T | 022 ‘T CSG-L8E | SPP 8S-€€ OF Oli WP yea er a %GO ‘ourpoyg ‘ST-¥ ‘SI | 6 FT OT8 ‘9-029 ‘G | 092 ‘9 OTS ‘T-060 ‘T | 002 ‘T LSP-Z9E | OCP Z9-OF cy Oi ee ei caeee ne ae ouoN —yUM pojyuswmoetddns Wqs —podj soyvuliozqyy ‘OI-I ‘FI | 6 FI 020 ‘2-022 ‘S | O21 ‘9 | OZF ‘T-OGO ‘T | O&Z‘T | SLZP-OTE | ZIP 09-68 | LP oak, Vinee xanga a > ne oe addds ‘OT-O FI | VST OFS ‘L-08Z ‘G | OFT ‘9 06 ‘T-000 ‘T | OST ‘TL €9F-LVE | OOF 9¢-6E LY UGA eli. eee ee We Ce Pe dds ‘LI-* FI | SST OOF ‘2-089 ‘G | 06Z‘9 | OOG ‘T-OFT ‘T | 082 ‘T GOS-TEs | SOP 6S-LE | LP Pam Ae ee ee i Wds ‘OI-9 FI | € ST O6T ‘2-020 “G | 002 ‘9 OSS ‘T-096 O61 ‘T IL¥-OLE | FOF LS-6€ Lv ate i|iaeaiebs Sea Re et HdS8 ‘SI-6 FI | O'LT OFg ‘2-002 ‘S | OEE ‘9 009 ‘T-OLT ‘T | 0¢¢ ‘T 90S-T0€ | €L4& f9-8E LY Tome hile = oO ee OAH 8 dS —prj $0} BULI09 VT ‘OIF ‘ET | € ST O6T ‘2-020 ‘¢ | OFT ‘9 OSE ‘T-096 OST ‘T TL¥-OLE | OOF 69-6E OF Sie a ile « © ree ee ye ent Ads ‘61-6 FT | 8 9T OFS ‘2-028 ‘F | 0G0 ‘9 009 ‘I-OF0 ‘T | 062 ‘T 90S-EL¢6 | O9E o9-8E SP PG ae. |i; es ape eee en ay OAH 8 dS $91.0]D/) S$91L0]D) SULDLE) SULDLY) SULDLE) SULDLE) SUDLE) SUDLY) aque NAT —pvj IV syetl WHA asUeyy ISBINOAY ddUBYIT OSBIDAY aSUBY aseIoAy | osuny |oseioay | osuvy | odeIoAy Szyey JoIp pues UIBIYS ules wield SyooM Z[—-oyeyUI SYOOM ZT YSIy SyooM ZT Say QYySIOM -lod-salloyeEg DILOTBI SSOINH dYVIUL POO UIVS JUSIOM SUIUBI AA spp snoiupa paf syou Bunoli ur urob fo wosb sad sarsojpo pup ‘aypjur poof ‘Yjnogg—S ATAY J, 16 g 6 NoRTa} TS Taeci: ‘OI-4 FI ‘LT-0 ST ‘02-G “ST SS ISSSST ‘02-Z@ “ST ‘SI-I “ST ‘6I-L OT iOTSS 71 ‘ST-I “ST ‘SI-G FI LO K o 092 ‘S-082 ‘F 0&2 ‘9-OLT ‘F OOF ‘S-060 ‘F 06 ‘S-082 ‘F OSF ‘S-019 ‘F OF8 ‘F-008 ‘€ 09% ‘9-022 “¢ 029 ‘9-092 ‘F 096 ‘9-09T ‘¢ 029 ‘9-026 ‘F 046 ‘9-O&T ‘¢ 00 ‘¢ OLT ‘¢ OIL 'F 00¢ ‘¢ OOT ‘¢ OOF ‘F 028 ‘¢ 008 ‘¢ O12 ‘9 098 ‘¢ 00 9 OOT ‘T-026 0ZE ‘T-068 0&L OL8 092 099 002 ‘T-O10 ‘T 0OL6 —0S¢ —002 —0¢9 —OT¢ —00L OLE ‘1-066 062 ‘I-026 OVE ‘T-066 6ZP—-9CE O8E—-99G IVE-LES GGE-ELS PSE-ZIS PEE—GIST 90E-S8T SOF—-608 ESE-EVS PLVVEE 80P-E8G 69P-FIE POE VEE 666 STE 082 £0 8hS EGE 962 OOF CGE LLE €G-LE Go-6E 9S-8E 6P-68 TL-SP €9-6& 89-68 6S-IP €S-6E TS-8€ 1G-68 6S-6& SP SP OT Or ST Or —poy soyeur9} yy] $y@d Jest —pay $o}vurs19}}1] JON yea Wore ‘ornyxtur y[es+ 16H eS Fa es te napa ere eT MddS SS tO Ce ae (330 Yselj) Ads —pe2j Soyeuse} qT 17 investigators reported that early growth of young pair-fed rats was consistently less when the diet contained 10 or 28 percent butterfat than when it contained a comparable level of corn oil. When the level of butterfat was increased to 35 percent, rats were as large as or larger than those on diets containing 10, 28, or 35 percent corn, soybean, or coconut oll. More efficient utilization of the diets was observed as the level of butter was increased; no such relation was noted for corn oil. Pair feeding and ad libitum feeding gave similar results. Hoagland and Snider (92) observed a growth- promoting value of peanut oil (gain per 100 Calories) similar to that obtained with lard or hydrogenated cottonseed oul when the level of fat was 5 percent. At 30-percent levels, diets con- taining lard were used more efficiently than either hydrogenated cottonseed oil or peanut oil. Aaes- Jorgensen and Dam (1, 2) included peanut oil in many of their investigations, and in general found similar growth-promoting qualities for lard and for peanut oil. The increase in growth rate that resulted when the fat level was increased from 7 to 28 percent was not due to increased caloric intake. Body weight in relation to age and diet in adult rats SP 8 HVO, SPE, SPM, SPB, anp SPPB piets.—Differences were observed in the rate at which adult rats gained weight and in the maxi- mum weight eventually attained, even when diets produced a similar growth response in young animals. In table 9 are summarized data showing the influence of diet on body weight at different ages. Included also for ready reference are the more extensive data already considered for SP 8 HVO and SPE diets. Adult rats fed the diets containing milk, beef, or peanut butter continued to gain throughout their healthy lifespan and were consistently heavier than rats of similar age fed the semipurified diet. They also tended to become heavier than rats fed the SPE diet. The older surviving rats fed SPM or SPPB diet tended to be larger than those of comparable age fed SPB diet. Differences in caloric intake did not account for some of the weight differences observed. For example, between 500 and 600 days of age the rats fed SPM diet gained, on the average, 70 grams, whereas those fed SPB diet gained 22 grams. The intakes for rats fed these two diets were simil: and 9,340 Calories respectively. Additional data on the caloric intakes of these rats at different ages are included in appendix table 80. KIND AND LEVEL oF FAT.—Differences in the weights of rats fed diets containing HVO, lard, or butter seemed to be attributable chiefly to differences in caloric intake except for rats fed 16 percent lard. At 300 days of age on a similar caloric intake, the average weight of rats fed semipurified diet was 60 grams less than that for the corresponding littermates fed SP 16 lard. 18 LEVEL OF PROTEIN AND FAT.—The caloric con- sumption of rats fed SPa 16 HVO and SPb 8 HVO Was approximately 5 percent more than that of rats fed the semipurified diet, and accounted for the tendency to larger animals on these two diets. Eee AND EGG COMPONENTS.—No appreciable change in the weight curve of adult rats resulted from replacing 30 percent of the protein of SPa 16 HVO diet with egg white (SPEW). When egg yolk supplied all of the fat in the diet (SPEY), weight changes for the adult, like those for the young rat, resembled those observed with SPE diet. Rats fed 100 percent egg (E100) remained relatively small but did eventually reach weights similar to those of stock rats. On SPW diet, ‘Tats tended to be small throughout life although the weight of the older rats was Ss somewhat oreater than that of the rats fed E100 diet. On 100 percent egg yolk, food consumption increased with age, so that weights of rats surviving 400 days were comparable to those of SP 8 HVO rats. WISTAR RATS FED SP 8 HVO anv SPE piets.— Wistar rats fed SP 8 HVO and SPE diets showed a somewhat different age-weight relationship than did BHE rats. On the semipurified diet, Wistar rats were consistently smaller than BHE rats of comparable age; on SPE diet, Wistar rats even- tually became much heavier. Discusston.—Reports in the literature on diet in relation to weight throughout the life of the adult rat are limited and, as with the growing rat, deal chiefly with the level or kind of dietary fat. No reports have been located dealing with protein- fat-containing foods such as have been included in this publication. In general, the results obtained in this laboratory and elsewhere provide consider- able evidence that differences in weight gain are not necessarily associated with differences in caloric intake. With mature rats 205 to 212 days old, Forbes, Swift, Elliott, and James (66) and Forbes, Swift, Thacker, and others (68) observed that the energy expense of utilization of isocaloric diets decreased as the level of dietary fat (chiefly lard) increased from 2 to 30 percent. A reduction in heat from the catabolism of carbohydrates and from fat synthesis was responsible for the resulting economy of utilization of food energy as the level of fat increased. French, Ingram, Uram, and others (69) reported that by the time rats were 28 weeks old on a diet in which 20 percent of the stock diet was replaced with corn oil, their weight exceeded significantly that of animals fed a diet in which 20 percent of the stock diet was replaced with sucrose. Lundback and Stevenson (1/9) obtained a gain of 0.6 gram per day for adult rats fed a diet containing 60 percent fat chiefly as lard, in contrast to no weight gain for rats on a comparable caloric intake of a diet containing 71 percent sucrose. *peqyVoIpUl Sosv ayy JB JYUSIOM SUIUTRIUTVUT [YS S]VI JO JeqUINU dy} OJBOIpPUL SesoyjuoIed UI SIOqUINN ‘OfI]T INOYSNOAYY PoYwoIpuUl SJoIp oY} UO POUTVJUIVUL S}VI OJ 1B BIVG | | (€) TLL 902 (9) 889 c99 | (4) 289 | (8) Ogg (Z) 669 |(¢) F9g (2) Sho | (8) STS £6F oLP ee (1) 109 | (#) Sse | (2) OTS e32--5-2--|---+--5+--|------2--- (9) gg¢ GPS ets Sees. See (2 0G ™ Ose Lit |..C8) CLF v6P Bee ees Se eae Sea (e)e7eo sO) 629 969 car wes (Z) 829 (F) 209 | (4) 199 | (8) @T19 66 eeeasoesks Se ale == el be 980) WCE). 99 419 = Saar a (¢) £69 (¢) #89 | (4) 829 | (6) 29 92S a oa (1) 182 (Z) 999 | (€) 889 | (8) zE9 909 Sir aaa (1) 209 (F) Z9L | (24) 029 409 eg¢ ere s2=-"==-==|\(e)<7ee> |e) Yeo | (9). O6¢ $68 eis ee (1) T¢¢ (G) 629 | (2) $09 | (8) 209 | (6) 86g Saga (1) OS (Z) 699 | (¢) 689 | (2) OT9 OF9 Pie Ee ChE PT (F) 799 | (F) Ze9 | (9) 66g +09 ery (@) $02 (¢) g¢9 | (2) Ze9 | (6) 929 L19 Saar (@) 88¢ (F) 22g | (9) 89¢ | (6) 69¢ 18g oe (z) OT8 (G) 832 | (9) 799 | (6) $¢9 |(ZT) 8T9 Spree (S) 934 (F) 299 | (¢) e99 |(OT) Ze9 609 ee ici ee (9) GLL (9) z€2 | (2) 689 |(TI) 299 9 ~---------/S25-205_.- (1) OSS | (Z) SIS | (F) PLE 868 (1) e6¢ |(z) F2¢g (€) ZS | (2) 999 (OL) F8E |(eT) Tg¢ Soo a Soe (€) G6¢ |(€T) €09 |(¥E) FZ9 |(69) LES (€) 819 |(2) 109 |(1%) 6z9 |(Se) G09 |(Zh) Sse |(ZG) TEE SUDL) SUWDLY) SUDLY) SWDLO SUWDLE) SULDLD) sdep 008 | Shep 004 | Shep 009 | skep 00g | SAep OOF | Sep 008 —1 38 FYSTIOM Cie | 86g e1Z SF OW | Rieibe Genacire wets Ads ccp | 9g e61 SF OULD Sek eee rae OAH 8 dS —pyj $0} BUI} 4VT SJV1 IVISIA (6) 69% | eTE LEI GP (ens | asi a ia OOTA 6cr | LIE 061 6S i naa eae nae a MdS — po] 8e7yVULI9}4}1] JON err | zee OST CF Ohl en ak Sta die eee OOTa $8S | P&P 91Z CF (Oe Riera ol ae eee Adds Ife | 8ge $02 FF (0) | Galen pa es MUdds og | SIF C1Z GF O12 = Ne Saas Se (389 Yselj) Hds —peoj SO}VULIO} YT L0G | 688 902 LP (Senge cme Sea gene OAH 8 4d8 (6) 89 | LIP 102 OF (0) eee cag 2 ee ae OAH 91 ®d8 L8¢ | SLE 10Z OF (0) oa | aes OAH 8 dS —p2} SOYBVULIOP IVT 61g | 78e Z61 Gr Oe” se ees ~1099nq 9T dg FOE =| 89 68I +P Olney So cas ee as Joyyng 8 dg gg | SOF $02 +P (Nin (teehee se ear pie] 91 dS 91g | ese 102 GF (i SS Se eS are PleT 8 dg Ges | 00F C0Z +P Oat |e ends OAH 91 dS IZ | O88 161 +P OR Blirie se oa OAH 8 dS —p9j soyvurI044yWT (81) 899 | ZtF 02% OF A neice Pegioeereece, S Mineo Neca ge dddg sec | O&F SIZ oF Pile seas Eo Ge Eat eee oe eee ads (81) 99¢ | 6aF 602 OF Filho Mase Dias Pee CS a ea a Wds LES | S&F 12% 9F Pe a Gar ae ice Se ace eae Ads F6r | 668 602 9F Pilg arg aoe ae kaos SS ee oy OAH 8 dS —podj soyeul194TT (€2) Ze | OF TOG OF 2 Raed tee em cic ears tarts a Ads 96F | F8E 002 oF CG Ne See Fane) nay OAH 8 dS SUWD.L) SULDLY) SULDLE) SUD) | LaQUNNT —p2J IIV sel THE skep 00Z |sfBp OOT | Shep OG JYSIOM SuluveA | $I 4yoIp pue ured spaup snoipa paf sab yuasaffip yo syou fo yybvam fipog— 6 WIV J, 19 Calories for maintenance of body weight BHE rarts.—Although many of the animals tended to gain weight throughout most of their lifespan regardless of diet, there were often 50- to 100-day intervals when weight remained rela- tively constant. The caloric intake during these intervals served as a basis for calculating calories required for maintenance. ‘The results available are recorded in table 10. The values were ob- tained for rats varying considerably in weight, but in general included data for rats on each diet with a similar weight range. On the basis of the more extensive data obtained for rats fed SP 8 HVO diet, the calories required for maintenance appeared to decrease with increasing age and/or body weight. With rats fed SPE diet, however, no such relationship appeared to exist. The tendency for heavy rats on those diets with the lowest maintenance requirement suggests that there may be a real difference in the way that these diets are utilized. The differences observed in the average calorie requirements with diet, however, were relatively small considering the wide range of values observed for individual rats. More data under controlled conditions of intake and activity are needed to establish the significance of these trends. Tasie 10.—Calories per gram of body weight per week for maintenance of adult rats fed various diets Calories per gram of body weight Strain and diet Rats Average Range BHE rats Number SH geil a RAO Bee ee ee 26 0.98 | 0. 82-1. 08 Dik wes oe Re oe 24 95 . 73-1. 14 SPs se ee Eee ee 8 90 . 76—- . 99 le ae 2 ee en 4 96 . 938- .98 SPPRBt.2 22 22ee ts feda. 6 90 . 84— . 95 Seil6é BVO... 222522 8 95 . 85-1. 13 Sie S lard: eee eee 8 92 . 80-1. 05 SPO lard 222.2 6 89 .79- .95 Db So butberve 252.22 8 92 . 89- . 99 SP WG buttere 22325 8 91 . 79-1. 04 Wistar rats DE SHELLY © tees ace 7 287 . 838- . 96 SP He wie eee 5 . 79 .73- . 85 Wistar RATS.—The lower calorie requirement for maintenance of the Wistar rats fed SP 8 HVO or SPE diets when compared with BHE rats parallels the greater efficiency in their use of these diets during early growth. In the young rats, the differences were due in part at least to the somewhat smaller body weight that was being maintained during this period by Wistar rats. A comparison of the weight and intake of the two strains of rats fed SPE diet (appendix table 80) shows a lower intake even by older Wistar rats 20 during periods when the weight was as great as or ereater than that of BHE rats. Discussion.—Little information is available on the energy requirements for maintenance of the adult rat. Fixsen and Jackson (62) estimated the requirement of the rat at 12.0 metabolizable Calories per 100 grams per day for animals weigh- ing 375 grams or less, and 11.5 metabolizable Calories per 100 grams for those weighing more than 375 grams. With two strains of rats, Palmer, Kennedy, Calverley, and others (151) demon- strated a difference in the utilization of foods for erowth and in the energy requirement for mainte- nance. The high-efficiency strain of animals stored a larger proportion of their food energy and lost less energy as heat than did the low-efficiency strain. These authors, using 4.1 Calories per gram for protein and carbohydrate and 9.3 for fat, ob- tained a value of 13.2 Calories per gram per day for maintenance of the high-efficiency rats and 14.6 for the low-efficiency animals. Application of these factors to the data reported in this publication for BHE rats fed SP 8 HVO diet re- sulted in a value of 12.7, similar to that of the high-efficiency strain. Wistar rats appeared to be still more efficient in their utilization of this same diet for maintenance. Maximum body weight and diet Average weight curves provide little informa- tion with regard to the variation in the rate of gain or in the maximum weight attained by indi- vidual rats. In table 11 are summarized data on the average maximum weight and the range of values observed for rats sacrificed between 300 and 500 days of age and for those that were older than 500 days. These values include data for rats scheduled for sacrifice in the age groups indi- cated, as well as those from longevity studies, and thus represent a relatively larger group of animals for some of the diets than are included in table 9. Data are also summarized for rats fed the various supplemented SPE diets and for a small group of rats fed SP 8 HVO and SPE diets from parents raised on the Wistar stock diet. Although the average maximum weights ob- served were generally higher, particularly for the younger group of rats, than were apparent from the average weight-age relationships seen in table 9, the results show the same general trend that has already been discussed. Rats fed SPE diet with the various supplements were similar in size to those fed the unsupplemented diet. When BHE rats were fed the stock diet on which the Wistar rats had been fed, weanling rats tended to be small and generally reached a maximum weight on SP 8 HVO or SPE diet that was less than that observed with these same diets when the young were from parents raised on the usual stock ration. Dirt AND OBESITY.—Large rats were observed with all of the experimental diets, particularly with SPM or SPPB diet. The largest rat was a TaBLeE 11.—Mazimum weight of rats fed various diets, sacrificed before or after 500 days of age Strain and diet Rats | Average age Number| Days BHE rats Stockman acre sek PRINS ee ee ee ee ee 31 416 SPeSeEVViQ seen tee erie. ne ti ae te 52 380 Protein-fat-containing foods SR piece ott We Me eae 131 392 SIR Mien he se ed eee ate 20 408 SRB eo tas & are Si SAR 19 410 SIPRBS ite ae Stes cw ee ook ds 26 394 SPE supplemented with— Choline 0:59. 2 222-225-228 16 419 By, 0.01 mg./100 gm_____-__-------- 6 412 Choline, 0.5%-+ By, 0.01 mg./100 gm_ 6 398 Be, 0.5 mg./100 gm____---_--------- 4 408 Choline, 0.5%-+ Be, 0.5 mg./100 gm_- 9 412 Choline, 0.5%+ Bry, 0.01 mg./100 gm.+ Be, 0.5 mg./100 gm_____--- 8 408 Cholesterol, 0.46%__.------------- 7 416 Cholesterol, 1.88%__..------------ 6 402 Ascorbic acid, ODO jp ities ae 5 377 Ascorbic acid, 0.2%-+ cholesterol, QUA C per ee ee ol ee RL 8 394 Fat, 8% or 16%: SRALG REV Ome Als ET 29 409 Sy Siler Be ceieyat a t e s ls 6 429 SIREIGi area eee Wee Ly lady ae 4 416 SPrstbutters22 2 eee os ae ee 2 394 SPelGibutterss se ee ewes 6 409 SP 8 HVO with protein or fat to level SBRalOveViOes: ewrece 85 sso 4 454 Fell 24 obtoy al UNA © Miah ere apap 3 456 Egg and egg fractions: SPli(Greshtege) es ess ee Ole 6 429 SRA re Ee ire ek Daeg 1 459 Ry EURO AYA sts oh Spr ea ee 3 407 SIR HYVeuiige inde Were i Pole 5 420 TSB ICO) dc gl ee 8 451 ATMO) Oise a EAD SC Ce ae ee 14 402 Y97+salt mixture, 3%-__---------- 5 430 Diet reversal: Stock throughout life-___.________-- 1 420 SPE throughout life___._._______-- 2 396 Stock changed to SPE at 250 days__ 0 SPE changed to stock at 250 days____ 1 384 Wistar rats SRsStEV OMe Okara St oy 1 446 ISIAH eva eee an fe ye! UL oe a 2 355 BHE parents fed Wistar stock diet BHE young fed— SIRUSHELV Oe eine ee et Ont erels SIR Bopanna A ety | ee Mae 3 358 Sacrificed at 300-499 days Sacrificed at 500 days or over Weight Weight Rats | Average age Average| Range Average| Range Grams Grams Number| Days Grams | Grams 470 370-579 57 680 484 | 399-670 602 423-771 57 636 663 | 514-890 626 456-790 65 575 645 | 505-792 684 575-791 21 649 753 | 85-1,020 615 486-800 23 618 690 | 565-902 680 510-976 28 581 715 | 610-908 659 548-756 8 584 670 | 583-790 630 573-713 3 566 713 | 666-780 648 511-764 3 562 730 | 668-767 664 565-790 4 570 666 | 645-713 654 590-735 I 508 623 | 623 661 605-723 2 522 624 | 564, 685 648 568-856 3 552 660 | 544-820 644 536-765 2 578 665 | 627, 703 630 510-740 3 535 596 | 500-769 645 551-781 2 531 689 | 600, 778 663 565-780 27 611 709 | 593-875 613 550-684 4 673 676 | 627-722 686 630-766 6 652 675 | 599-797 600 597, 602 7 662 645 | 564, 732 627 593-662 4 644 624 | 528-700 651 571-736 15 696 725 | 555-982 635 501-845 % 689 674 | 524-870 633 566-729 4 562 687 | 566-788 524 524 6 550 574 | 440-668 598 497-651 7 652 692 | 601-791 668 582-744 5 560 666 | 590-763 541 442-668 17 555 541 | 424-698 522 448-698 6 539 589 | 532-646 555 SV S—5 95 | eee | eee re ee ee tee | eee 537 537 2 675 612 | 528, 697 554 530, 578 1 629 825 | 825 SLES De ees (Ere Coreg eerd |e rer oe 4 686 694 | 608-791 535 535 3 705 680 | 643-709 445 445 9 772 576 | 475-673 538 519, 673 iG 791 758 | 588-970 Bad is Loan oem | had aR achat 9 759 561 | 482-684 533 446-583 5 592 565 | 510-616 No extremely large Wistar 720-day-old animal fed SPM diet that weighed 1,020 grams. Among the rats 500 days and older that were fed this diet, four reached weights exceeding 900 grams. On SPPB diet, one rat reached a maximum weight of 976 grams by 341 days, and three additional animals that were less than 500 days old reached maximum weights exceeding 800 grams. rats were obtained with the semipurified diet, but with SPE diet one rat weighed 970 grams at 750 days of age, exceeding by “178 grams the largest BHE rat fed this diet. No quantitative data on body composition were obtained for the studies reported in this 21 bulletin, but animals that were obviously obese were obtained with several of the experimental diets. Marshall, Hildebrand, Dupont, and Wo- mack (126) observed for adult rats an apparent difference in the conversion to body fat of calories from different diets, even on comparable calorie intake. Apparent differences in the utilization of calories by the adult rat fed some of the diets such as SPM or SPPB may be related to the extent of the conversion of calories from these diets to body fat. Discussion.—Mickelsen, Takahashi, and Craig (132) observed exceedingly obese rats when the Osborne and Mendel strain of animals were fed ad libitum a diet containing 60 percent Crisco. The largest animal weighed 1,655 grams. Sprague Dawley rats or NIH black rats fed these high-fat diets became heavier than stock animals but not so heavy as the Osborne and Mendel strain. Obese rats approaching 1,000 grams in weight were also observed when the Osborne and Mendel strain were fed the authors’ “best” low-fat regimen for a period of 60 weeks. The weight curves of these rats fed the low-fat diet (3 percent) were similar to those already discussed for BHE rats fed many of the experimental diets. A reduction in growth rate occurred at about 15 weeks, with many of the rats continuing to gain slowly throughout the 60- week experimental period. Some rats showed a spurt in body weight gain at the 20th or 30th week. This ‘best’ low-fat diet of Mickelsen, Takahashi, and Craig (132) was a relatively simple diet with casein as the chief source of protein and with a protein level of 25 percent. Sucrose was the carbohydrate in this diet, amounting to 66 percent. The sucrose content of the semipurified diet and its various modifications discussed in this publica- tion was high (39 to 52 percent) and may be re- sponsible for the ready acceptance of these diets by BHE rats. Food intakes frequently were as much as 19 or 20 grams daily even at a relatively early age. The high digestibility and correspond- ingly low fecal bulk observed by Marshall, Hilde- brand, Dupont, and Womack (126) with similar diets make it possible for rats to consume excessive amounts of these diets without apparent digestive disturbances. In general, the results obtained with BHE rats provide further evidence that obesity in normal male rats may be produced by diet and that rats may overeat voluntarily if sup- plied with diets that are sufficiently acceptable and that can be consumed in relatively large amounts. The failure to obtain equally large rats when the stock diet was fed was probably due to a limitation in the amount of this diet that could be consumed because of the large fecal bulk and the poor digesti- bility observed with this diet (126). Longevity In table 12 are summarized data dealing with the survival of rats on all of the experimental regimens under investigation. In addition to the average 22 results for the more extensive investigations with SP 8 HVO and SPE diets, data are also presented for individual series to permit a direct comparison of the response of littermates to those diets for which only limited information is available. SP 8 HVO pier.—A group of 53 rats representing five experimental series on the semipurified diet provides data on the influence of this diet on longevity. The average age of the animals at death was 629 days, with 50 percent dead by the end of 616 days. Fourteen rats survived more than 700 days. Figure 4 shows the percentage of rats that died within different age intervals on this diet. The highest death rate occurred between 600 and 700 days. SPE piet.—Similar data were obtained for 85 rats representing eight experimental series on SPE diet (table 12). The lifespan of these rats was considerably shorter than that observed with the semipurified diet; average age at death was 464 days, with 50 percent dead by 449 days. Only one of the 85 rats survived more than 700 days. As shown in figure 4, the maximum death rate for these rats occurred between 400 and 500 days of age. PROTEIN-FAT-CONTAINING Foops.—Two series of rats were fed the diets containing milk, beef, or peanut butter. In addition, comparative data were obtained on the response of littermates to SP 8 HVO or SPE diets. The results for the 21 littermates fed SP 8 HVO or SPE diets were, in general, similar to those already discussed for the larger number of animals fed these diets. The shortest lifespan was observed for animals fed SPE diet. Rats fed the SPPB diet also tended to die at an early age. Although the average age at death was approximately the same—about 580 days—for rats fed SP 8 HVO, SPM, or SPB diets, the number of rats dying within comparable age intervals was not the same. Figure 5 presents data for SPM, SPB, and SPPB diets similar to that seen in figure 4 for SP 8 HVO and SPE diets. On SPM diet there were several early deaths but 8 of the 21 rats survived more than 700 days, in contrast to only 3 of the littermates fed SP 8 HVO diet. On SPB diet only 1 rat died before reaching 400 days of age; the maximum death rate occurred between 600 and 700 days; 4 rats survived beyond 700 days. On SPPB diet the maximum death rate occurred at a somewhat earlier age, between 500 and 600 days, with 5 of the 21 rats dead before 400 days of age. SPE DIET WITH SELECTED SUPPLEMENTS.—Rats fed SPE diet supplemented with choline, vitamin B,, or vitamin By, alone or in combination, were generally as short lived as on SPE diet alone, and there was no evidence that any of the supplements investigated had a measurable effect on the longevity of these animals. The addition of cholesterol or ascorbic acid also seemed to exert little influence on the length of life of rats fed SPE diet. TABLE 12.—Age at death and mortality rate of rats fed various diets Strain and diet Littermates fed— SPE supplemented with— By, 0.01 mg./100 gm______ Choline, 0.5%+By, 0.01 migs/VOOvem 22 ee Littermates fed— SPE supplemented with— @holine;:0:5% =. 2. = Be, 0.5 mg./100 gm_________ Choline, 0.5% + Bg, 0.5 mg./ Choline, 0.5% + Be, 0.5 mg./ 100 gm. + By, 0.01 mg./ NO Of orm se eke eee SS Littermates fed— ee supplemented with— Cholesterol, 0.46%________ Cholesterol, 1.38%_--_-_-_- Ascorbic acid, 0.2% ______- Ascorbic acid, 0.2%-+ cho- lesterol, 0.46%_______.__- Littermates fed— Littermates fed— SPE (fresh egg)___.-________ SPEW E100 IS GOC Keser aia eye ue cada oy) Age at death Rats dying before— Rats 50 per-| Old- Aver-| cent est 400 500 600 700 age | died | rat | days | days | days | days by— Num- Per- | Per- | Per- | Per- ber | Days | Days | Days | cent | cent | cent | cent 53 629 616 917 2 17 43 73 85 464 449 705 28 64 88 99 21 579 574 889 5 24 57 86 21 467 419 705 38 57 76 95 21 580 578 903 19 43 57 62 21 589 603 852 5 29 43 81 21 525 541 722 23 37 66 85 8 444 435 581 25 62 1OOR| Sasa 8 441 414 551 38 88 100) |e e 8 475 431 632 25 62 88 100 8 423 400 642 38 75 88 100 9 458 426 626 33 78 89 100 9 432 410 581 22 89 1000 |252—--— 9 464 423 625 33 56 89 100 9 427 418 508 22 79 O08 2e22== 9 443 438 527 22 78 HOO e222 8 415 375 597 50 88 10Q S22" 2 8 477 474 578 25 62 100K See 8 410 406 574 38 88 LO0Mes.22e 8 405 361 533 50 75 TOO} je22 = 2 8 414 358 520 50 88 LOOM ess o= 9 631 643 774 0 22 33 67 9 618 569 775 11 11 44 67 9 524 444 706 11 67 67 89 9 553 506 776 D2, 44 56 78 9 602 610 774 11 22 33 78 9 514 487 707 33 56 67 78 16 618 614 889 6 25 44 69 16 566 518 799 12 44 56 81 8 676 623 917 0 0 25 75 8 582 522 810 0 38 62 75 8 593 505 813 0 38 50 88 18 680 653 917 0 11 28 56 18 654 621 893 0 Live 38 61 10 482 465 604 10 60 90 100 10 578 552 749 10 30 50 70 10 490 441 585 10 50 HOO ass 2 2 10 548 525 696 10 30 70 100 25 559 522 762 8 32 64 92 19 393 381 591 58 74 TOO! | eee 3 590 |e wees 727 0 33 33 67 3 v1 ss Sep 629 67 67 67 100 4 G86s|2 22 2 2 805 0 0 25 25 4 624 Joes 767 25 25 25 50 800 days 900 | 1,000 | 1,100 days | days | days Per- | Per- | Per- cent cent cent 96 OOF See ae LOO} 2 sess | eer 95) 100 |o. - TOO) 52235 |eeass UOOs | 2eees | Sean 88 100) 222222 MOOS |Se ae |b eee LOOW| 22a. |b2ee 89 1007 === VOOR see So) eee OO) | Saas |e TABLE 12.—Age at death and mortality rate of rats fed various diets—Continued Age at death Rats dying before— Strain and diet Rats 50 per-| Old- Aver- | cent est 400 500 600 700 800 900 | 1,000 | 1,100 age oe rat | days | days | days |; days | days | days | days | days v= Wistar rats Num- Per- | Per- | Per- | Per- | Per- | Per- | Per- | Per- Littermates fed— ber | Days | Days | Days | cent | cent cent cent | cent cent cent cent OEE iO tee 28 eee ee ae 10 739 836 876 0 10 20 40 40 LOOM Sense Dy Boe. See eee ae 10 654 742 888 30 30 40 40 60 LOO 222224 |seeee— BHE parents fed Wistar stock diet Young fed— Ser VO) 2. 2 0 oe tea 9 703 755 |1, 028 11 11 33 33 78 78 78 160 S124 0 eee eee ee ee eee Pee 9 424 381 762 44 56 89 89 LO Oe) oer = | eee DIET: SP 8 HVO DIET: SPE % DYING % DYING | 30 20 10 NONE eee 0 Paci® 9 2 Ss 4.” SS C: Hees AGE (hundred days) Ficurn 4.—Percentage of total number of rats dying within different age intervals on SP 8 HVO and SPE diets. KIND AND LEVEL OF FAT.—Data available, al- though limited, suggest that the lifespan of rats may be influenced by the kind and/or level of fat in the semipurified diet. When the dietary fat was HVO, the average age at death was approxi- mately the same whether the level of fat was 8 or 16 percent. Although there appeared to be no difference between the survival of rats fed diets 24 containing 8 or 16 percent lard, the lifespan of both groups was less than that of animals fed the diets containing HVO. When butter was the dietary fat and the level was 8 percent, the average age at death was close to that observed with HVO but decreased when the diet contained 16 percent butter to approximately that observed when lard was the dietary fat. DIET: SPM < 4 AGE (hundred days) Figure 5.—Percentage of total number of rats dying within different age intervals on SPM, SPB, and SPPB diets. Sait, OO LS The average length of life of rats fed SP 16 butter diet was less than that observed on SPM diet. The type and level of protein in these two diets were the same; mineral content as well as level of fat was similar. The major differences between these diets were the presence of 6 percent HVO and of 5 percent lactose from the dried skim milk in SPM diet, whereas all of the fat in SP 16 butter was from butter and all of the carbohydrate was sucrose. The presence of lactose may have been a factor contributing to the difference in the survival on the two diets, although the amount was low in comparison with the 39 percent sucrose in this diet. No antioxidants were added to these diets, and the tendency for somewhat re- duced consumption of the SP 16 butter diet may be related to lower stability of the fat in this diet. The content of the low-molecular fatty acids (Cy, and below) in the SP 16 butter was higher, about twice that of the SPM diet. Data available pro- vide no answer as to the cause of the differences observed. PRotTEIN LEVEL.—Modification of the semipuri- fied diet by increasing the protein or the protein and fat level to that of SPE diet resulted in no significant change in the lifespan of these rats. Respiratory infection was responsible for some of the early deaths of the small group of eight litter- mates fed SPa 16 HVO and SPb 8 HVO diets; no difference in survival was apparent for the larger group of littermates fed SP 8 HVO and DIET: SPB DIET: SPPB 6 T> < 4 5 6 1 ? SPa 16 HVO diets. The results with SPb 8 HVO and SPa 16 HVO were similar, suggesting again that increasing the level of HVO from 8 to 16 percent was without effect on survival. EGG AND EGG COMPONENTs.—Rats fed a diet containing a relatively high level of egg white (SPEW) and those fed a diet consisting of 100 percent whole egg survived longer than those fed a diet containing 25 percent egg (SPE) or 30 per- cent egg yolk (SPEY). The lifespan of rats fed SPEW was similar to that observed for those fed the modified semipurified diet (SPa 16 HVO) with comparable levels of fat and protein, whereas the average age at death for rats fed SPEY diet was similar to that obtained for littermates fed the SPE diet containing cooked fresh egg. The survival period of rats fed 100 percent egg yolk was short. No data were obtained on longevity of rats consuming 97 percent egg yolk and 3 per- cent salt mixture, but other results obtained with this diet, to be considered more fully later, suggest that the lifespan of rats fed this high egg yolk diet may be increased appreciably when the diet is supplemented with a suitable salt mixture. Although some of these data seem to indicate that it is the yolk of egg that is contributing to the shortened lifespan of rats fed SPE diet, the finding that rats tolerate diets containing 100 percent egg better than a diet containing either 25 percent whole egg or 30 percent egg yolk 29 indicates that other dietary ingredients also are contributing to the response of rats to SPE diet. Drier REVERSAL.—The number of rats included in the series to investigate the influence of reversing stock and SPE diets at 250 days of age was small, and details for the individual rats are summarized in table 81 of the appendix. The harmful effects of the SPE diet appear to have been overcome or prevented for 3 of the 4 rats that were changed from SPE to stock diet at 250 days of age. The 4th rat was losing weight before the diet was changed and died shortly thereafter. This sug- gests that irreversible damage had already occurred before the change in dietary y regimen at 250 days. The average age at death of the 3 animals that were maintaining their weight at the time of the change in diet was 705 days, in contrast to a life- span of 473 days for rats continuing on SPE diet throughout life. Three of the 4 rats placed on SPE diet after 250 days of age lived more than 700 days, in marked contrast to 1 out of a total of 85 that reached this age when SPE diet was fed throughout life. These findings suggest that the harmful effects of SPE diet are due to a stress imposed upon the young rat, and that this diet may be well tolerated by the adult rat. HEREDITY AND RESPONSE TO SP 8 HVO or SPE prer.—Wistar rats lived longer on both SP 8 HVO and SPE diets than did BHE rats. Over 50 percent of the rats fed these diets lived more than 700 days, in marked contrast to the results with BHE rats, particularly those fed SPE diet. Although the average age of survival of Wistar rats was somewhat less on SPE than on SP 8 HVO diet, the difference was of questionable significance considering that the early death of two of the rats fed SPE diet was due to respiratory infections and seemed unrelated to diet. The shortened lifespan that resulted from feeding SPE diet to BHE rats when their parents were raised on our regular stock diet also occurred when their parents were raised on the stock diet that had been used for the Wistar animals. Genetic differences, therefore, and not differences in dietary history of the parents appear to be responsible for the short survival of BHE rats fed SPE diet. Stock prnT.—No systematic data were collected to determine the longevity of BHE rats fed the usual stock diet, but apparently these animals are relatively long lived. Among the rats that were sacrificed to provide information on the characteristics of stock animals at different ages were 10 rats over 800 days of age. Three of these were still maintaining their weight and appeared to be in good health at 820, 916, and 976 days of age. The remaining 7 animals, from 808 to 897 days old, were losing weight at the time of sacri- fice. Likewise, BHE rats fed the stock diet that had been used for raising the Wistar rats were long lived. Five rats that were continued on this diet to provide data on older animals were still maintaining their weight when sacrificed at 26 an average age of 938 days. No information was obtained with older Wistar rats fed their usual stock diet. EARLY WEIGHT GAIN AND SURVIVAL.—Although the experiments reported in this bulletin were not designed to determine the influence of food con- sumption and/or weight on survival, they afford considerable indirect evidence indicating that ex- cessive food consumption and the accompanying rapid gain in body weight were important factors in determining the lifespan of these rats. In table 13 are summarized data for gross caloric intake by rats fed SP 8 HVO and SPE diets during the first 300 days of life, with the results separated into groups based on the age at which consistent weight loss began. The average intake on both diets tended to be less, particularly during the 200- to 300-day period, for rats with the longer span of healthy life, although there was consider- able variation in the food intake of individual rats. All except 1 of the 9 rats fed SP 8 HVO diet with a caloric intake of 1,900 Calories or more lost weight and showed signs of ill health before they reached 600 days of age. Considering the simi- larity in the caloric intake of rats fed SP 8 HVO and SPE diets, however, it was apparent that differences in the lifespan on these two diets were not due to differences in the amount of food eaten by these animals. The adverse effect of rapid weight gain in the young adult rat is still more apparent from the data shown in figure 6. Curves 1 and 2 contrast the growth curve of a group of rapidly growing rats fed SP 8 HVO diet with that of rats of rela- tively long lifespan; curves 3 and 4 represent similar data for rats fed SPE diet. The 14 rapidly growing rats fed SP 8 HVO diet all had attained a body weight of 550 grams by the time they reached 200 days of age; 4 exceeded 600 grams in weight. The average age at death of these rats was 553 days. Curve 2 shows the slower growth rate of 14 rats that were fed the same diet and survived 600 days or more without weight loss. Most of the rats in this latter group weighed less than 500 grams at 200 days of age, and none had reached a weight of 550 grams. The average age at death was 812 days. The 16 rapidly growing rats fed SPE diet all exceeded 600 grams in weight by 200 days, with an average age at death of 423 days. By 600 days, most of the rats fed SPE diet were dead or were losing weight, regardless of early weight gain. Curve 4, therefore, represents data for rats maintaining or gaining weight at 500 days of age. Their average lifespan of 623 days was oreater than that of the rapidly growing rats fed the same diet, but did not equal that of rats growing at a comparable rate when fed SP 8 HVO diet. Figure 7 represents similar data for a small group of rats fed SPM, SPB, and SPPB diets, and provides further evidence for the harmful effects of early rapid gain in body weight. The curves for the most rapidly growing animals fed these TaBLeE 13.—Mazrimum age of rats before weight loss in relation to caloric intake during the first 300 days on SP 8 HVO and SPE diets Intake during— Diet and age of Average rats (days) Rats | healthy 0-12 weeks 100-199 days 200-299 days lifespan Average Range Average Range Average Range Number| Days | Calories Calories Calories Calories Calories Calories SP 8 HVO: Less than 500__---_- 15 387 6,110] 5, 260-6,860 |} 8, 320 | 7, 190- 9, 780 8, 510 7, 100— 9, 870 HOOEtORD99 Sse 28 | 12 541 6,000 | 5, 220-6,960 |} 8,170 | 7, 280-10, 060 8, 220 7, 280— 9, 780 600 to 699________- 11 624 5,870 | 4, 840-6, 720 | 7,760 | 6, 160- 8, 930 7,900 | 6, 530— 9, 020 a 700 and over___---- 6 757 5,590 | 5, 030-5, 920 7, 890 | 6, 670— 8, 410 7,450 | 6, 720— 7, 990 PE: Less than 400_____- 20 330 | 6,180 5, 540-7,120 | 8, 260 | 6, 760-10,180 | 8, 610 7, 060-— 9, 290 400 to 499_____.-.. 11 423 | 6,330) 5, 690-6, 820 | 7,940 | 6,170— 9,140 | 8,210] 6, 690-10, 180 500 and over___-_--- 6 533 | 5,610 | 5, 020-6, 340 6, 830 | 5, 500— 8,100 | 6, 950 5, 570— 7, 730 DIET: SP 8 HVO DIET: SPE GRAMS 800 600 400 ; r : | | I | =o Obese rats g ox Obese rats | 200-4 (553 days*) i (423 days*) 4—A Long-lived ie A--A Long-lived rats (812 days*) rats (623 days*) 0 0 150 300 450 600 0 150 DAYS 300 450 600 DAYS * a vERAGE AGE AT DEATH FicurE 6.—Comparison of growth curves of obese rats fed SP 8 HVO and SPE diets with those of long-lived rats fed the same diets. diets are all for rats weighing 600 grams or more by 200 days. Of the 18 rapidly growing rats fed these three diets, 8 were dead before reaching 400 days of age; only 1 survived 600 days (603 days on SPB diet). The rats that were still maintaining their weight at 600 days of age grew slowly on all three diets and survived approxi- mately 300 days longer than did the rapidly growing rats fed the corresponding diet. The slowly growing rats tended to reach maximum weights comparable to those attained by the rapidly growing rats but at a much older age. _ No data were available from this study to indicate the possible advantage of restricting food 721-631—64——_3 intake to prevent the excessive weight gain of the older rats fed these diets. ‘The tendency for rats to become heavy at an early age when fed SPM and SPPB diets may be a factor in the relatively large number of early deaths on these diets. Al- though a rapid gain in weight was generally asso- ciated with a short lifespan, a slow rate of early gain did not necessarily result in a long, healthy life. The results with other experimental diets show a similar trend for rapid early growth to be associated with early death, but are too few to permit their separation as has been done for the diets just discussed. The tendency to a shortened 27 DIET: SPM DIET: SPB DIET: SPPB GRAMS 7 800 = | ie pes ue A- pes 600 PL pane 4a a we i Oo Obese rats (382 days*) A--A Long-lived rats (682 days*) o—o Obese rats (437 days*) A--A Long-lived rats (775 days*4) 2001-4 o—o Obese rats (456 days*) A--A Long-lived rats (737 days*) 0 150 300 450 600 0 DAYS 150 300 450 600 0 DAYS *,VERAGE AGE AT DEATH 150 300 450 600 DAYS Fiaure 7.—Comparison of growth curves of obese rats fed SPM, SPB, and SPPB diets with those of long-lived rats fed the same diets. survival period for rats fed lard (SP 8 lard and SP 16 lard) may be due in whole or in part to excessive consumption, along with the tendency to more efficient utilization of these diets. Discussion.—There have been extensive inves- tigations on the nutritional requirements of the rat, but the majority of them have dealt with the period of early growth. Relatively few have covered the entire lifespan. Many of the early experiments concerned with longevity have dealt with diets deficient in one or more nutrients, result- ing in retarded growth and in premature death. Although growth has been the basis for studying nutritional adequacy of many diets, considerable evidence has accumulated to indicate that rapid growth in early life may not insure optimum health throughout life. McCay (120) was the first to demonstrate that lifespan of rats could be extended by severely restricting caloric intake while maintaining adequate levels of essential protein, minerals, and vitamins. With much less restricted intakes (33 and 46 percent) and without severe retardation of growth and sexual maturity, Berg and Simms (27, 22) showed an extension of life expectancy and a delay in the onset of major diseases. Ross (166, 28 167), investigating the effect of uniform lifelong dietary regimens on the mortality pattern of rats, demonstrated the possibility of modifying life expectancy not only by quantitative dietary restriction but also by the ratio of the protein and carbohydrate components in the diet. Riesen, Herbst, Walliker, and Elvehjem (158) obtained a beneficial effect on survival when the caloric intake of rats fed a synthetic diet was restricted. Carlson and Hoelzel (41) found that intermittent fasting tended to increase the lifespan of rats under conditions that did not result in drastic retardation of growth. Everitt and Webb (59) reported for male rats that the faster an animal reached its maximum weight, the sooner it deteriorated and died. Callison, Orent-Keiles, and Makower (40) compared the results of feeding human-type diets with rats fed a stock ration. The stock diet which produced the smallest early weight gains resulted in adult animals with the lowest maximum weight and in the best physiological condition. In contrast, the fast- growing animals which attained the highest maximum weights were inferior in physical condition, as judged by bronchiectasis and skin condition. McCay, Maynard, Sperling, and Osgood (121) found that the maximum lifespan of their animals occurred when rats weighed between 350 and 450 erams. No animal with a body weight exceeding 450 grams at any time in life lived more than 810 days. In considering nutrition during the latter half of life, these authors reported that the degree of fatness of the body is the most important factor as far as lifespan is concerned. Silberberg and Silberberg (/71) reported the results of feeding male mice a stock diet containing 5 percent fat with or without an additional 25 percent lard. The mean lifespan of the mice consuming the fat-enriched diet throughout their life was 107 days shorter than that of the stock-fed animals. When the high-fat regimen was initiated at the age of 6 or 12 months, the lifespan was also shortened but less so than when this regimen was started at weaning. When fed for 5-month intervals, the results varied, depending on the period of life during which the fat-enriched diet was consumed. The differences observed were due to high fat and not to the caloric intake. French, Ingram, Uram, and others (69) com- pared the results of ad libitum feeding of a diet containing 22.7 percent fat, chiefly corn oil (20 percent), with one containing 3.4 percent fat and 20 percent sucrose in place of the corn oil. The lifespan of male rats fed the high-fat diet was markedly decreased. Decreased lifespan was cor- related with increased efficiency of utilization but not with caloric intake. These authors indicate that this decrease in longevity may have been due to the fat as such, or may have been the result of the improved growth rate conferred by the high- fat diet. According to Comfort (42), heredity may be as important as dietary reduction in determining lifespan. Although the lifespan of laboratory animals can be increased by eliminating specific heritable diseases, inbred stock rats tend to reach a shorter lifespan than do random-bred animals, and the author suggests that information on the mechanism for vigor in hybrids may prove of significance in studies of aging. Sperling, Loosli, Barnes, and McCay (176) also reported data im- plicating inheritance as a factor in survival. Six and one-half percent of the litters of rats investi- gated by these authors accounted for 27 percent of the short-lived rats that died within 500 days; 18 percent of the litters accounted for 47 percent of the animals dying after 700 days. Lane and Dickie (111) presented data showing the shortened lifespan that results from excessive food consump- tion by genetically obese mice fed ad libitum and, as the result of long-term restriction of food intake of these mice, increased their lifespan by more than 300 days. Summary In general, growth was good on all of the experi- mental diets under investigation, and no evidence of any dietary deficiency was apparent. Young rats tended to grow more rapidly when the level of fat was 17 to 19 percent than when the diet contained 9 percent fat or less. With some diets, growth rate was associated with caloric intake; with others, such as those containing high levels of egg, milk, beef, or peanut butter, it appeared to be associated with efficiency of utilization. Rats fed stock rations generally attained their maximum weight at a relatively early age and maintained a constant weight thereafter. On the semipurified diets and the various modifications of it, rats tended to continue to gain throughout their healthy lifespan. Rats exceeding 800 grams in weight were frequently obtained, especially on the diets containing milk or peanut butter. The results reported provide further evidence of the many factors that need to be considered in evaluating the effect of diet on the lifespan of the rat. Survival of rats varied even on diets of simi- lar fat and protein content. The tendency to excessive consumption of certain diets may explain some of the differences in survival observed. The extent of weight gain and the amount of a diet that can be tolerated by the adult rat without ad- verse effects appear to vary with diet. Diets con- taining high levels of egg or egg yolk resulted in a shortened lifespan, but the longer life of rats fed 100 percent egg indicates that other dietary in- eredients were also contributing to the response to the SPE diet. The results obtained when stock and SPE diets were reversed at 250 days contribute further evidence that the age period under study may be a contributing factor in the response to diet. The results of feeding the same diets to BHE and Wistar rats emphasize the importance of recognizing inherited characteristics in evaluat- ing dietary response. Histology and Size of Selected Organs Histology of kidney When subjected to gross and to microscopic examination, the kidneys from 113 rats fed the stock diet, 99 fed SP 8 HVO diet, and 201 fed SPE diet, provided information on the influence of diet, age, fasting, and weight loss on this organ. Rats MAINTAINING WEIGHT ON sTocK, SP 8 HVO, ano SPE piets.—In table 14 are sum- marized for different age groups the results of eross and microscopic examination of the kidneys from rats that were maintaining weight on these three diets at the time of sacrifice. The results for fasted and nonfasted animals have been com- bined. There appeared to be a tendency toward somewhat higher ratings for the presence of hyalin casts when rats were sacrificed without fasting, but the differences were too small to warrant a separation of the results without more data to establish the significance of this trend. 29 Taste 14.—Kidney damage determined microscopically for rats maintaining weight at different ages on stock, SP 8 HVO, and SPE diets Diet and age of rats Rats Gross (days) rating Hyalin Stock: Number Score Score Less than 200_.....-- 19 0 200't0: 29908220525 18 2 300 to 399___________ 11 .6 400 to 499___________ 10 .6 . HOO tO 690 ras eS ata .8 i 600 and over_____---- 16 1.0 iti: SP 8 HVO: Less than 200_______- 5 4 200 to: 299.255. Lecce 12 a3 300 to 399_____-___-- 8 a? 400 to 499___________ 7 <4 500't0:599 222-2 --c 9 .8 SPE: Less than 200______-- 5 ce? 0 200 to 299__________- 23 :8 1. 300 $0: 399 22cecce ses 12 1.6 1. 400 to 499__________- 14 125 al HOO t0' 599 2. 2 eis 9 2:2 de In general, the kidneys of rats under 200 days of age were normal on all three diets. On stock or SP 8 HVO diet extensive degenerative changes in the kidney were rarely seen. A _ gradual increase occurred with age in the number of rats fed stock or SP8 HVO diets, with kidneys showing the presence of eosinophilic albuminous material or hyalin casts. Kidneys from 81 percent of the rats fed the stock diet that were over 600 days old had a hyalin rating of 1 or more, although one of the oldest rats (916 days) had a kidney that was apparently normal. Cystic or glomerular damage was found occasionally and was seen more often in the kidneys from rats fed the stock diet than from those fed SP 8 HVO diet. On SPE diet the results were in marked con- trast. Of those animals that were fed this diet and were more than 200 days old, relatively few had normal kidneys. Kidneys with hyalin ratings of 2 or 3 were observed even in rats 200 to 300 days old. Cystic or glomerular damage was occasionally seen but was generally slight. Calcium deposits were not observed in the kidneys of rats that were maintaining weight on any one of these three diets. RATS LOSING WEIGHT ON stock, SP 8 HVO, AND SPE prets.—Both age and weight loss were found to influence the type and extent of kidney damage observed, and the data summarized in table 15 have been separated accordingly. For rats fed the stock diet and with weight loss less than 100 grams, little damage was observed in the kidneys from the few animals that were less than 700 days old; extensive degenerative changes were observed in kidneys from rats over 700 days old, with 40 to 75 percent of the kidneys showing cystic and glomerular damage as well as hyalin 30 NONWH Histological rating NUR RDO Or ar Kidneys with— Cystic |Glomerular) Hyalin Cystic |Glomerular Score Score Percent Percent Percent (0) 0 0 0 0 0 11 0 0 1 Pee 18 9 9 a 2 50 10 10 4 5) 64 27 18 4 4 81 19 31 0 0 20 0 0 0 0 17 0 0 0 0 38 0 0 0 0 29 0 0 3 0 56 11 sal 0 0 0 0 0 mal . 04 78 0 4 4 wel 92 33 8 al 2 64 14 21 3 3 100 33 33 casts. When weight loss exceeded 100 grams, 86 percent of the kidneys showed all three types of damage and the extent of the damage observed bore no relation to age. When cystic damage was large, there was a tendency for a reduction in hyalin casts. Fibrosis was generally apparent when all three types of damage were evident. Calcium was present in only one of these kidneys. Animals fed the semipurified diet with weight loss less than 100 grams presented a somewhat different picture from that of animals fed the stock diet. ‘The most extensive damage was found in kidneys from the small group of rats between 500 and 600 days of age; kidneys from animals sur- viving over 600 days tended to show progressively less damage with increasing age. Relatively little cystic or glomerular damage was observed. When weight loss exceeded 100 grams, only 2 of the 29 kidneys from rats 400 days of age or older were normal; no consistent relation between age and extent of damage was observed. Cystic and glo- merular damage was found in 65 percent of these kidneys, a marked increase over that seen in rats losing less weight but somewhat lower than that found for comparable groups of rats fed the stock diet. Calcium deposits were found in the kidneys of 41 percent of these animals and appeared to be the heaviest between 400 and 600 days of age. Calcium was apparent only when all three types of damage were present. The data for fasted rats were too few to determine whether or not the nutritional state of the rat at the time of sacrifice was a factor in determining the presence of calcium in the kidneys of these rats. On SPE diet, relatively few normal kidneys were observed, regardless of age or weight loss. For rats under 400 days of age and losing less than 100 TaBLe 15.—Kidney damage determined microscopically for rats losing weight at different ages on stock, SP 8 HVO, and SPE diets Weight loss, diet, Histological rating Kidneys with— and age of rats Rats Gross (days) damage Hyalin | Cystic |Glomerular| Calcium | Hyalin | Cystic |Glomerular| Calcium Rats losing less than 100 grams: Stock: Number | Score Score Score Score Score | Percent | Percent | Percent | Percent 400 to 499____ 1 0 0) 0 0 0) 0 0 0 500 to 599__-_- 2 2 9 0) 0) 0 50 0 0) 0 600 to 699____ 2 1. 0 1.0 5 0) 0) 50 50 0 0) 700 to 799____ 4 2.2 2. 0 1.0 1.8 0 75 75 75 0 800 and over__ 5 2. 4 1.8 1.0 1.4 0) 100 40 60 0 SP 8 HVO: Less than 300_ 4 0 0 0 0 0 0 0 0 0 300 to 399____ De 0 0 0 0 0 ) 0 0 0 400 to 499____ 7 .6 9 0 0 0 57 0 0 0 500 to 599____ 5 2.3 2. 0 1.0 4 0 80 40 40 0) 600 to 699___- 6 14 5 ne, nes 0 33 ile 17 0 700 and over_-_ 3 oh nO 0 0 0) 33 0) 0 0 SPE: Less than 300_- 6 a) vit 0 2 0 50 0) 17 0 300 to 399____ 21 1.8 1.8 9) er sul 95 29 38 14 400 to 499____ 18 2.7 2. 2 1.8 1.2 3 94 ed 72 17 500 to 599____ 9 3.8 1.9 2. 6 1.3 7 100 89 78 44 600 and over_- 8 Baz 245 2. 0 1.6 4 100 88 62 12 Rats losing more than 100 grams: Stock: 300 to 399____ 2 4. 0 2. 0 2.5 3. 0 LO 100 100 100 50 400 to 499____ 2 3.5 2.5 DED 2. 0 0 100 100 100 0 500 to 599____ 3 3.3 20:7, 3. 0 27) 0 100 100 100 0 600 to 699____ if 4.0 4. 0 2.0 3. 0 0 100 100 100 0 700 to 799____ 4 2.0 2. 0 .8 1.5 0 100 50 75 0 800 and over_- 2 3.5 2:5 2.0 3. 0 0 100 100 100 0 SP 8 HVO: Less than 300_- 2 0 aD 0 0 0 50 0 0 0 EXOD) Troy aH ONS ye | een Ds aca Io sw] DO a Eo | S| en | DSC OE | (a 400 to 499____ 4 3. 5 2.5 2.8 1.8 1S 100 15 75 50 500 to 599___-_ 9 24 251 11 1a, 1.2 100 56 67 44 600 to 699____ {6 2.3 2. 0 1.9 LZ .6 86 el il 43 Spee and over_-_ 9 3. 0 2d 14 1.3 3 89 67 56 22 Less than 300__ 1 4.0 3. 0 1.0 1.0 2.0 100 100 100 100 300 to 399____ 19 3.9 2.50 3. 2 2. 0 2.4 100 95 95 79 400 to 499____ 23 3.8 Qik 3. 0 2.1 19 100 100 100 74 500 to 599____ 21 Sil 20) 3.3 1.9 1.3 100 100 95 62 600 and over_- 12 3.8 PA) 2.9 2.1 .9 100 100 92 42 grams in weight before sacrifice, the extent and kind of damage found was similar to that already seen (table 14) for rats that were fed this diet and were maintaining or gaining weight. In the older rats, however, there was an increase in the extent of cystic or glomerular damage. When weight loss exceeded 100 grams, no normal kidneys were seen and more than 95 percent showed glomerular and cystic damage as well as hyalin casts. Degenera- tive changes were extensive regardless of age, and the scores for cystic damage tended to exceed those for glomerular damage, in contrast to the results with rats fed the stock diet. Calcium was present more frequently in the kidneys of rats fed SPE diet than in those fed SP 8 HVO diet ; 18 percent of the kidneys showed evidence of calcium deposition when weight loss was less than 100 grams; 62 percent, when the loss exceeded 100 grams. Data on SPE diet were sufficient to permit a further evaluation of the factors influencing the occurrence of calcium deposits in the kidneys. In table 16, the results of microscopic examination for calcium are considered in relation to age, weight loss, and nutritional status of the rat at the time of sacrifice. Calcium was found occasionally in the kidneys of rats losing less than 100 grams and was frequently found in the rats losing more than 100 grams. It was observed more often in kidneys from nonfasted rats than in those from fasted rats, regardless of weight loss. When weight loss was under 100 grams, 9 percent of the kidneys from fasted rats and 32 percent from nonfasted rats 31 TaBLe 16.—Cailcium determined microscopically in kidneys of fasted and nonfasted rats losing weight at different ages on SPE diet Fasted Nonfasted Weight loss and age of rats (days) Kidney Rats with Kidney Rats with Rats calcium calcium in Rats calcium calcium in kidneys kidneys Rats losing less than 100 grams: Number Score Percent Number Score Percent Ness: then 3002 a = ee ee 4 0 0 2 0 0 BOOMO) 699" 2. a4 = ne coun ae as ee eo 12 0 0 9 aS, 33 40010400". 6 coe to ee 13 ae 8 5 .6 40 DOO TOLD OO row ees ae eee a oe 8 .6 38 1 ae A0) 100 OOO MndtoVvier?.2. 3. ee 6 0 0 2 ees, 50 Rats losing more than 100 grams: Hest Fl aY: Rass) 0,0 So Ne Ae ET ee IO [aCe RE a NEB i | NS See am AMR Es Ne REAL 1 2. 0 100 300 40-690. Se. oe oo oe eee 7 1.4 Lys 12 3. 0 92 BOD tO 4098 oo eet a eee ne Se 10 1.6 60 15 2.0 93 DOOM O99 soos ea ee oe 12 28 50 9 2.0 78 G00sand Over: 5.22 a ee 7 at 43 5 1, 2 40 showed evidence of calcium deposits; when weight loss was more than 100 grams, the corresponding percentages were 53 and 83. Calcium was found most frequently and in the greatest amounts in the kidneys of nonfasted rats 300 to 600 days of age that had lost more than 100 grams. Although fibrosis was not necessarily accompanied by calcium, calcium was observed only in kidneys showing extensive damage with evidence of fibrosis. Rats FED SPM, SPB, anp SPPB piets.— In table 17 are summarized data from the micro- scopic examination of kidneys and livers from rats fed diets containing milk (SPM), beef (SPB), or peanut butter (SPPB). The results for rats that were maintaining weight did not differ mark- edly from those for rats that had lost less than 100 grams in weight before sacrifice, and the limited data for these two groups of rats have been com- bined. No separation by age was necessary when weight loss exceeded 100 grams. In rats maintaining or losing less than 100 grams in weight, the chief evidence of degenerative changes in the kidneys was the presence of hyalin casts. On all three diets, the kidneys of rats under 300 days of age were generally normal in appearance. On SPB diet, little evidence of kidney damage was observed except in rats 500 days of age or older, whereas hyalin casts were a frequent finding in the kidneys of rats 300 to 500 days of age fed SPM or SPPB diets. When weight loss exceeded 100 grams, kidney TasiLe 17.—Kidney damage determined microscopically for rats maintaining or losing weight at different ages on SPM, SPB, and SPPB diets Weight loss, diet, and age of rats (days) Rats Rats maintaining weight or losing less than 100 grams: SPM: Number Wess when 0O2. 2. SS hs SOU eee 16 BOO TO: 499 8. 3 Sa 12 DOO BndsoVer: 2s). fw Re ee 9 SPB: bess than o002 2-222 2 eo oon ee 16 SOO0M4OvA99) 2 ol ee ee ae es 12 p00 and Over: 25.- 5 es 8 SPPB: Less than /300 220s se ge es 19 S000 4990 ufo ee ee 21 500: and overs=- 222-5 L224 8 eee 16 Rats losing more than 100 grams: oH 2A, Ee ee een Sree 14 ae yee 2 ee 15 EB ees a a ne te ne ae 13 Histological rating of kidney Average age . ‘ Hyalin Cystie /|Glomerular| Calcium Days Score Score Score Score 217 0. 4 0 0 0 353 .8 a 32 0 577 raid .2 .2 0 206 mAb 0 Al 0 400 ae) 2 22 0 627 1,2 aD <2 0 217 3 2 Al 0 387 1,2 .6 .6 0 597 0 2 api 0 624 1.4 1,2 aiff .6 556 2.3 2.1 1.3 Beal 512 2.7 2.5 lea wo 32 damage was generally present, regardless of age. Kidneys from rats fed SPM diet showed the least evidence of degenerative changes. In spite of extensive damage, calcium deposits were rarely seen in the kidneys of rats fed SPPB diet although, otherwise, these kidneys were similar to those from comparable animals fed SPE diet. With regard to calcium deposition, the kidneys from rats fed SPB diet resembled more closely those from rats fed SPE diet. RATS FED OTHER EXPERIMENTAL DIptTs.—In table 18 are summarized the results of micro- scopic examination of the kidneys from rats fed all of the other experimental diets. The data were obtained chiefly from rats that were sick or moribund. The influence of weight loss on the extent of kidney damage was seen consist- ently in all of the experimental series, but for most diets the data were too limited to permit a separation of the results on this basis. The results reported, therefore, include all available data, along with the average weight loss for each group to aid in comparing the results of the various experimental regimens. None of the supplements investigated were able to prevent extensive degenerative changes that occur in the kidneys of rats fed SPE diet. How- ever, the data summarized in table 18 do show some trends suggesting that these supplements may have influenced the metabolic processes TABLE 18.—Kidney damage determined microscopically for rats fed all other diets Strain and diet Rats BHE rats SPE supplemented with— Number @holineQ0i5Y Sos eee oe ee sees 22 By 0-0F met/l00:'¢m_ =~ _ 222-22 Uf Choline, 0.5%+ By, 0.01 mg./100 gm_________ 10 Be Ocorme. /MOOsemes2 Ue ie SE 8 Choline, 0.5%-+ Bs, 0.5 mg./100 gm__________ 8 Choline, 0.5%+ Bi, 0.01 mg./100 gm.+ Bg, Oloemes/ OOF emis ool ea ee ee ek ee 1 Cholesterol 0:46 9, 2 ee ee eee @holesterol#il38 Qe v2 28 fas he Ss ee Ascorbic acid?:0:2 22 .- 2 2 228 eal ee Ascorbic acid, 0.2%-+- cholesterol, 0.46%___-- SP 16 HVO RM rg (ee) — i) ied Q. ! 1 1 1 i) 1 1 1 ' i] 1 1 1 1 ' 1 i) 1 ' ! i] 1 1 1 } ' \ 1 1 ' 5 ' i] — RM ar) i) a lor) an) 2-8 == 11 258 466 1. 67 1. 38-2. 01 . 36 27 54 18 0 300 to 399__________ 5 376 482 2.01 1. 34-2. 96 .4l 20 40 40 0 400 to 499__________ 7 465 486 1, 87 1. 07-2. 70 . 88 14 57 29 0 500 to 599__________ 11 538 490 2.15 1. 41-3. 61 . 43 9 54 27 9 600 and over________ 11 679 457 2. 23 1. 45-2. 93 47 9 9 §2 0 SP 8 HVO: Less than 200______- 5 154 521 2. 08 1. 79-2. 54 . 40 0 60 40 0 200; tOrZ99e Sem ies ae 6 248 532 2. 02 1. 43-3. 32 Sol 17 50 Ale 17 300 to 399__________ 23 332 636 1. 88 1. 41-2. 71 . 80 13 61 26 0 PE: Less than 200_______ 5 154 520 1. 92 1. 76-2. 17 Nan 0 60 40 0 2005¢0:299 os 18 27. 613 2; 22 1. 49-4. 19 . 37 6 22 67 6 3000 B99n eee 29 330 614 3. 01 1. 69-8. 88 .49 0 38 38 24 1 Weight before 17-hour fast. the percentage of rats with large livers and not to an occasional excessively heavy liver. The increasing difference with age between both the kidney and the liver weight of fasted and nonfasted animals suggests that a slowing of the metabolic processes has occurred in the older rats, resulting in a temporary enlargement of these organs, which can still return to normal after a 17-hour fast. In fasted rats fed SP 8 HVO diet, livers showed a consistent tendency to increase in size with age, in contrast to the relatively constant values ob- served for the weights of livers from comparable stock rats. The livers represented 2.1 percent of body weight, with no apparent influence of age on this relationship. The increase in liver size with age, therefore, seems to be due chiefly to the tendency for adult rats fed SP 8 HVO diet to continue gaining in body weight with age. Although the livers from these animals weighed more, on the average, than those from stock rats, they were significantly smaller in relation to body weight (P<0.01). There was no evidence that age was a factor within the limited range for which data were available. Livers from nonfasted rats averaged 20.3 grams in weight, 8 grams heavier than those from fasted rats of corresponding age. In nonfasted rats fed this diet, large livers were observed even in rats less than 200 days old. In contrast to the stock rats, the livers from these nonfasted SP 8 HVO rats tended to represent a smaller percentage of the body weight with increasing age, 4.5 percent for rats under 200 days, and 3.1 percent for those between 300 and 400 days of age. On SPE diet, the livers from fasted rats were larger than those from rats fed stock or SP 8 HVO diets, averaging 21.0 grams in weight and 3.4 per- cent of body weight. The range of values was wide, from 12.8 grams for the smallest liver to 31.8 39 TABLE 23.—Liver weights in fasted and nonfasted rats maintaining weight at different ages on stock, SP 8 HVO, and SPE diets Liver weight Rats with livers weighing— Average Condition, diet, and Average| weight Liver to age (days) Rats age at body Less | 10.0 to] 15.0 to| 20. 0 death ! | Average Range weight | than 14.9 19.9 | grams 10.0 | grams | grams} and grams over Fasted rats Stock: Number | Days Grams | Grams Grams Percent | Percent|Percent| Percent| Percent Less than 200_-_--_--- 4 160 381 9. 6 8. 3-10. 2 2. 5 50 50 0 0 200 to 299.255 22° 15 248 447 115 8. 3-14. 3 226 20 80 0 0 300 to 399___-_-__--_ 6 382 428 V7 8. 6-16. 1 2.6 33 50 17 0 400 to 499__.-_______ 5 436 436 11. 2 9. 1-12. 2 2.5 20 80 0 i) 500 and over-.===2-.- 7 723 405 10. 7 8. 7-13. 6 2.4 43 57 0 ) SP 8 HVO: 200) t0 299 28 222 6 252 502 L110 9. 1-13. 9 2,2 33 67 0 0 300 to 399___..-_-_-- 8 353 541 12.0 9. 6-14. 1 202 12 88 0 0 400 to 499__________- r 457 600 13. 4 10. 1-18. 2 2. 2 0 ff 29 0 Pais GOVO99 2 eee 14 546 680 14.3 10. 8-17. 9 2.1 0 71 29 0 BOOMtOrA GO emt cs as 7 252 552 18. 2 12-8-24. 3 3.3 0 14 [al 14 300 to 399___-_-_---- 8 348 588 23. 6 18. 2-30. 1 3. 9 0 0 12 88 400 to 499___________ 14 455 616 21. 7 14. 3-31. 8 3.5 0 uh 29 64 500'(0:599-. 226222 1 530 624 20. 0 13. 5-26. 6 3. 1 0 9 46 46 Nonfasted rats Stock: Less than 200____---- 15 148 390 13. 3 9. 4-15. 8 3. 4 87 13 0 0 200 t0:299 2 a eae 11 258 466 15.1 12. 0-17. 8 3.2 46 54 0 0 300 to 399___-_______ 5 376 482 16.8 14, 2-21. 7 3. 4 20 60 20 0 400 to 499___________ 6 465 486 a eas 14. 4-25. 9 3. 6 29 57 14 0 D000 599. = a al 538 490 18. 6 13. 0-26. 9 3. 7 18 46 36 0 600 and over__-_---- 11 679 457 18.7 11. 7-21. 7 3.9 9 54 36 0 SP 8 HVO: Less than 200___----- 5 154 521 23.0 21. 2-30. 7 4.5 0 0 80 20 200 to 2992. 6 248 532 18. 7 14. 3-27. 2 3. 5 17 50 33 0 . 300 to 399___-______- 23 332 636 20. 0 14. 6-26. 3 3. 1 9 39 52 0 Less than 200_.__---- 5 154 520 22. 7 19, 2-27. 1 4.3 0 20 80 0 200 to:2992. 2 2k 18 277 613 28. 4 21. 8-36. 4 4.6 0 0 72 28 300 to 399___-.__-_-- 25 331 GL7 29. 9 20. 0-48. 1 4.8 0 0 60 40 1 Weight before 17-hour fast. grams for the largest. No consistent trend with age was noted except for the larger percentage of rats less than 300 days old with livers weighing less than 20 grams. The livers from nonfasted rats fed SPE diet averaged 28.6 grams in weight and represented 4.7 percent of body weight. For rats less than 200 days old, livers were large in comparison to those from rats fed the stock diet but similar in size to those from comparable SP 8 HVO rats. The largest livers obtained with stock, SP 8 HVO, or SPE rats were from nonfasted SPE rats 200 to 399 days old. CoRRELATION COEFFICIENTS.—The data in ta- bles 22 and 23 show that the factors affecting kidney weight generally exert a parallel influence on liver weight. In figure 8 are seen the lines ob- tained from the regression equation expressing the relationship between kidney and liver weights of fasted and nonfasted rats fed stock diet as well as 40 the data for individual rats. Food intake imme- diately before sacrifice was not controlled for the nonfasted rats and undoubtedly accounts, in part at least, for the greater variation in the relationship observed for these rats. For any one diet, liver and kidney weights tended to parallel body weight, and variations in body weight within age groups seemed to account in part for the wide range of values observed in the weight of these organs. Consideration of the indi- vidual data, however, indicated that the close parallelism between liver and kidney weights was not due entirely to the relation of the weight of these organs to body weight. In table 24 are sum- marized, for rats fed stock and SP 8 HVO diets, data relating liver to kidney weight and these organs to body weight. Correlation coefficients of 0.84 to 0.88 were obtained relating liver and kidney weights of fasted and of nonfasted rats fed these two diets. Correlation coefficients relating liver KIDNEY WEIGHT Fasted Regression equation: y=0.29+0.113x 0 4 8 12 Nonfasted o Regression equation: y=0.052+0.109x 16 20 24 26 LIVER WEIGHT (grams) Fieure 8.—Liver weight in relation to kidney weight in fasted and nonfasted rats fed stock diet. or kidney weight to body weight tended to be lower than those for liver and kidney, although for fasted rats the differences observed were generally small. The lowest correlation coefficients were those relating liver or kidney to body weight in nonfasted rats fed the semipurified diet. There was little evidence for a quantitative rela- tionship between liver and kidney weights or be- tween these organs and body weight in rats fed SPE diet. Even in fasted rats, ratios for liver to kidney weight varied from 3.4 to 15.5. KIDNEY WEIGHT OF ANIMALS LOSING WEIGHT on stock, SP 8 HVO, anv SPE piets.—In table 25 are summarized data on the kidney weights of fasted and nonfasted rats that were fed stock, SP 8 HVO, or SPE diets and were losng weight when sacrificed. The results for rats Josing less than 100 grams have been separated from those for rats losing over 100 grams in weight. When weight loss was less than 100 grams, the kidneys from fasted rats fed stock or SP 8 HVO diets were similar in size to those from rats of comparable age that were maintaining weight on these diets. On SPE diet, however, large kidneys were observed even in young rats, and in animals 400 to 599 days old 68 percent of the kidneys weighed more than 4 grams. When weight loss exceeded 100 grams, the kidneys from fasted rats tended to be larger with all three diets than did those from comparable animals with little or no weight loss. The largest kidneys were from rats fed SPE diet. The tendency of the nonfasted rats to have larger kidneys than the fasted animals was apparent among those that were losing weight, as well as among those maintaining weight, although the differences were small for older SPE rats when exceedingly large kidneys were found. In nonfasted rats losing more than 100 grams, kidneys were large at all ages regardless of diet; kidneys from SPE rats tended to be larger than those from animals fed stock or SP 8 HVO diets. LIVER WEIGHT OF ANIMALS LOSING WEIGHT ON stock, SP 8 HVO, anp SPE pints.—In table 26 are summarized data for the liver weights of rats that were losing weight on stock, SP 8 HVO, or SPE diets. In general, the results for rats losing less than 100 grams were similar to those for comparable rats that were maintaining weight except for the tendency to smaller livers in young nonfasted rats fed SP 8 HVO diet. When weight loss exceeded 100 grams, the difference between the liver weights of fasted and nonfasted rats became small, reflecting the reduced food intake of these animals. The livers from SPE rats were consistently larger than those from rats fed stock or SP 8 HVO diets. The marked increase in kidney weight that has been seen to occur with increasing Joss in body weight was not accom- panied by a parallel increase in liver weight. 41 TABLE 24.—Correlation of liver, kidney, and body weights in fasted and nonfasted rats fed stock and SP 8 Condition and diet Fasted rats: HVO diets Liver to kidney weight Rats Ratio Correlation coefficient Number 37 7.2 0. 88 34 7.6 . 85 59 8.9 . 85 33 10. 7 . 84 Liver to body weight Kidney to body weight Percent Correlation Percent Correlation coefficient coefficient 2:5 Oeil 0. 35 0. 72 201 . 80 . 28 . 60 BRD 74 . 40 60 3. 4 49 Rol 45 TaBLE 25.—Kidney weights in fasted and nonfasted rats losing weight at different ages on stock, SP 8 HVO, and SPE diets Weight loss, condition, diet, and age (days) 500 ay over be opts SP 8H Less hee DOO s.2'2.- 38 500 and over____------ SPE: Less than 400______--- 400 to 599_______---- 600 and over_______--- Nonfasted: Stock: Less than 500___-___-- 500 and over__-------- SP 8 HVO: Less than 500_-------- 500 and over____------ SPE: Less than 400_-_..---- 400 to 599_________---- 600 and overszoce.-. Rats losing more than 100 grams: Fasted: Stock: Less: than. 500.2222. -s2lewooscse SPE: Less than 400___-_-_- aes 400 to 599___________-_- 600 and over____------ Nonfasted: Stock: Less than 500___-___-- oe and over..-------- SP 8 HVO: Less than 500__-_-____- 500 and over____-_---- SPE: Less than 400.- 2222. AQO' to 599.2 2222.c0.- 600 and over______-_-- Rats Kidney weight Average age Average Range Days Grams Grams 718 1,89 | 1.62— 2 295 1. 30 8i- 1. 644 1. 84 | 1. 46— 2. 296 2.18 85- 7. 489 5. 73 | 1. 85-11. 643 3. 38 | 1. 79- 6. 456 ul 1: 787 2.72 | 2. 17— 3. 375 1.79 | 1. 33- 2. 645 3.28 | 1. 81- 6. 316 4.36 | 1. 51-13 498 5. 30 | 1. 78-12 608 8. 60 | 5. 78, 11 682 2.74 | 2. 09- 3 419 4.90 | 1. 53- 8 654 2.92 | 1. 86- 4 376 7. 74 | 3. 56-13. 504 6. 93 | 3. 37-11 644 4.98 | 2. 79- 7 411 4.50 | 2. 69- 8 695 3. 35 | 1. 65— 5. 426 3.35 | 2.17, 4 685 3. 81 | 1. 27- 7. 339 8.13 | 4. 80-12 486 7.14 | 3. 06-12 643 6. 32 | 3. 50- 8 Rats with Less 1.50 to than 1.50} 1.99 grams grams Percent | Percent 54 0 v1 71 véai 29 59 10 60 79 37 32 2 0 9 22 0 17 48 100 0 70 0 0 21 20 50 32 0 25 3 0 18 4 0 14 0 0 95 0 0 OL 0 33 49 0 50 2 (0) 0 6 0 0 O07 0 0 . 05 0 0 98 0 29 . 53 0 0 . 80 i 19 0 0 0 0 elod: 0 0 kidneys weighing— 2.00 to | 3.00 to More 2.99 3.99 |than 4.00 grams | grams | grams Percent | Percent | Percent ~ 4 29; #+#O| Oo 0 0 0 30 0 0 21 0 11 18 5 68 50 17 17 0 0 0 62 38 0 30 0 0 25 25 25 36 18 2h 14 29 43 0 0 100 <5 25 0 0 0 67 0 25 25 0 14 86 0 5 95 14 0 86 50 0 50 0 43 29 50 0 50 19 19 38 0 0 100 0 LS 85 0 20 80 42 TABLE 26.—Liver weights in fasted and nonfasted rats losing weight at different ages on stock, SP 8 HVO, and SPE diets Liver weight Rats with livers weighing— Weight loss, condition, diet, Rats | Average Less 10.0 15.0 to | 20.0 to More and age (days) age Average Range than to 14.9 19.9 29.9 than 10.0 grams grams grams 30.0 grams grams Rats losing less than 100 grams: Fasted rats: tock: Number | Days Grams Grams Percent | Percent | Percent | Percent | Percent NF Sse Lae Talay) (ecm ee Pape epee (vee eae a |) ee ee ee eee ee ee eee se |e 500 and over___--___- 6 712 12.5 10. 7-15. 5 0 83 74 0 0 SP 8 HVO: Less than 500_______- 7 295 9.4 6. 5-11. 7 OL 43 0 0 0 B00 andiover:.=2225-- 9 645 12. 4 9. 1-15. 4 11 78 11 0 0 SP ee than 400________ 19 296 16.1 7. 6-26. 0 16 37 21 26 0 400 to 599___________ 22 489 20. 7 15. 9-27. 0 0 0 50 50 0 600 and over____-___- 6 643 19. 6 16. 5-25. 6 0 0 67 33 0 Nonfasted rats: Stock: Less than 500_______- 1 456 10.3 10. 3 0 100 0 0 0 500 and over_____-___- 8 787 19.3 14. 6-24. 1 0 12 50 38 0 SP 8 HVO: Less than 500_-______ 10 375 14. 3 10. 0-23. 0 0 70 20 10 0 A 500 and over________- 5 619 20. 1 14, 0-24. 7 0 20 20 60 0 PE: Less than 400_______-_ 11 315 25. 4 15. 0-38. 7 0 0 18 55 27 400 tO: 599 ae a oe 6 489 26. 7 15. 1-388. 9 0 0 33 33 33 600 and over_________ 2, 608 43. 1 33. 6, 52. 6 0 0 0 0 100 Rats losing more than 100 grams: Fasted rats: Stock: Wessathaniys (0 Seeeaete eee |e ee | ee alee ree oan ee ee eee ee loa eee Sole See Le SIL ete aus and over_____-___-_ 4 682 15. 4 11. 9-18. 2 0 50 50 0 fe) SP 8 HVO: Less than 500_______- 3 419 14. 7 13. 1-18. 0 0 67 33 0 0 P 500 and over________- 4 654 13. 8 13. 8-14. 0 0 100 0 0 0 PE: Less than 400________ vé 376 21.2 14. 0-380. 2 0 14 43 29 14 A000 59 Ours aes 22 504 1OEe 14. 7-27. 5 0 5 55 41 0 600 and over________- 7 644 16. 5 14, 3-19. 8 0 28 72 0 0 Nonfasted rats: Stock: Less than 500_______-_ 4 411 19.8 14. 0-23. 1 0 25 0 75 0 poe and over_______--_ 7 695 16. 6 14. 6-19. 4 0 29 (fll 0 0 SP 8 HVO: Less than 500________ 2 426 14, 7 14. 2,15. 2 0 50 50 0 0 ae andeOveras =. 222 = 20 695 16. 0 10. 8-22. 4 0 35 50 15 0 Less than 400________ 13 339 20. 5 16. 4-24. 1 0 0 38 62 0 ANOKtO2599 zu ee oh sae L 25 482 20. 5 14. 3-40. 1 0) 8 48 40 4 600 and over________- 5 643 20. 2 16. 1-26. 9 0 0 60 40 0 KIDNEY WEIGHT AND DAMAGE.—Table 27 shows __ the presence of a few hyalin casts. When kidneys the relation of kidney size to the histological find- ings. Because of the influence of fasting on the size of this organ, the results of fasted and non- fasted rats are reported separately. For both fasted and nonfasted rats, a gradual increase in kidney damage with increasing kidney weight was observed with all three diets. Kidneys weighing less than 1.8 grams were generally normal in appearance. The only evidence of degenerative changes in kidneys weighing 1.8 to 2.0 grams was weighed between 2 and 3 grams, more extensive damage was apparent, with glomerular damage occurring in many of the kidneys from stock rats. Kidneys weighing more than 3 grams generally showed evidence microscopically of all three types of damage regardless of diet, but the ratings for glomerular damage were consistently greater for rats fed the stock diet. LIVER WEIGHT, LIVER FAT, AND KIDNEY DAM- AGE.—The weights of livers from rats fed the 43 TaBLE 27.—Kidney weight as related to kind and extent of damage in fasted and nonfasted rats fed stock, SP 8 HVO, and SPE diets Condition, diet, and range of kidney weight (grams) Fasted rats Stock: d fst 96,0) a: 0d | 0 pp ee ee ns ppt na. Tien a ges gape HUSOtO 100 2 ee eo a ee eae PRL ULE IR ro 16202 | Ne enn ae Seo ed ee Pine ep) en te ety BOO 00M 90 6 arose oc ke as ee SP 8 HVO: Mess: than esOUW oe ae eee a ee eee SOV BOWLED Oe aie ata als ee a, Dee Be 2 OObO 2. 00 = ed bees ee cei ee ee ee ay ND ts ae es a S:00-an dO veres2 2) a ee ee Less Hany 1 S02 aa eis ee Oe 2 ON eee ee ee eee ME OER OO pa oe nny hn eee Rene Rae, ee eee eee POO sO ne OO x nae on RS I a le a ee eee SPE Nonfasted rats Stock: SPSS day 0 Mn PS | 0 een em a rte Eee usenet © TSO) TO OO ret Nok Bo be Pe as en ee ee ee ee Se OOM 10 Oia oe a se het Pe a a ne SOON TO AOD am Rete ewe ee ene ee SO0camMC OVER: 2 yr ht ei coe we eyo a ae ee ee SP 8 HVO: Whésoathannd (S00 See Baas ee Dee ee 180 tLOsLOOLct 6a iF eae 8 eee ee ae FANON GO! DOO) eae rs Eo, atom, at heaping at gets es OO EONA OD te nok hae ce ae ee SPE stock diet also paralleled closely the extent of the damage observed in the kidneys. In table 28 are summarized data from nonfasted stock rats that were maintaining their body weight. Little evidence of kidney damage was observed in these rats when livers weighed less than 16 grams; ex- tensive damage was found when livers exceeded 20 grams in weight. ‘There were too few data to establish a comparable relationship in the fasted rats, and in moribund rats this relationship was complicated by variable weight loss. In nonfasted rats fed SP 8 HVO diet, larger livers were not necessarily accompanied by damaged kidneys. No evidence of kidney damage was apparent in the group of five rats 150 days of age, although three of the livers from these rats weighed more than 20 grams. On SPE diet no consistent relation was found between kidney damage and liver size or between size of liver or fat in the liver as seen microscopi- eally. A liver weighing 13.5 grams showed evidence of fat in amounts comparable to that 44 Average Histological rating Rats kidney | _ weight Hyalin Cystic |Glomerular Number Grams Score Score Score 25 1. 44 Weal 0 0 2 1. 89 1.0 0 0 7 2231 129 .9 1.4 1 PARTE 4.0 2. 0 3. 0 34 1. 44 2 0 0 7 1. 89 rl ) 0 6 2. 38 ez 2 .2 3 4,12 203 2.0 2.3 1 8. 67 3. 0 4.0 20 23 1455 mG) 0 0 10 1. 87 ileal 0 zo 25 2. 53 2.0 aD 2 16 4, 22 DAs 1.9 1D 43 7. 44 2.4 3. 4 200) 34 1. 47 a 0 0 10 1. 90 ai) aul 0 23 2. 38 1,4 “ial ah 9 Sa0L 3. 0 2.0 2.9 2 W202 2.0 4.0 3.0 12 1. 62 4 0 0 ih 1.90 6 0) 0 12 2. 34 de? 2 .2 1 4.18 2.6 2.2 1.8 5 6. 82 3. 6 3.0 2. 4 iA 1. 70 6 0 0 5 18h 1.0 0 0 Pall 2. 28 Hee, 0 aul 13 OAL 2.5 1.2 1.4 40 8. 52 2.4 wo 2. 2 TABLE 28.—Liver weight as related to kind and extent of kidney damage in nonfasted rats maintaining weight on stock diet Liver Aver- Histological rating weight age range Rat liver grams) weight | Hyalin | Cystic Glo- merular Num- ber Grams | Score | Score Score Less than 14.0 15 1235, 0 0 0 14.0 to 15.9___ 18 14.9 ai 0 0 16.0 to 17.9 __ 11 17.0 9 0) 0 18.0 to 19.9 __ 6 18. 6 i=5() Bass 3 20.0 and over. 10 22.8 2.1 2 1.6 seen in livers weighing 25 grams. Fat was apparent at an early age before kidney damage occurred, and was seen in all but one of the livers from rats with normal kidneys. As_ kidney damage increased, liver fat tended to decrease and often was too small to be apparent microscopically without special stains for fat. RATS MAINTAINING WEIGHT ON SPM, SPB, AND SPPB piets.—In table 29 are summarized data for the weights of the livers and kidneys from fasted and nonfasted rats that were main- taining their weight on diets containing milk, beef, or peanut butter. For comparison, data are also included for rats fed SP 8 HVO and SPE diets from the same experimental series. The younger fasted or nonfasted rats were littermates except for an occasional death before the age scheduled for sacrifice. The influence of fasting on the size of the kidney as well as the liver was again apparent in the results for the young rats. In general, the results for SPM, SPB, and SPPB diets were similar to those obtained with the semipurified diet except for the tendency to large livers in animals over 400 days old fed SPPB diet. For all diets except the SPE diet, an increase in liver weight with age was accompanied by a parallel increase in body weight, with the liver remaining a relatively constant percentage of the body weight. The tendency for certain litters to be particularly prone to enlarged, damaged kidneys was apparent in the group of rats 400 to 600 days of age. One litter accounted for the largest kidney recorded in table 29 for each of the diets except SPE. The littermate fed SPE diet was moribund at the time of sacrifice and had a kidney weighing 8.7 grams. The kidney weighing 5.57 erams from the littermate fed SPB diet was responsible for the high average ratio of kidney to body weight noted for this diet. RaTs LOSING WEIGHT ON SPM, SPB, anp SPPB pints.—Differences among these diets became apparent in rats that were losing weight, as is seen in table 30. Some extremely large kidneys were found in young rats fed SPPB diet, particularly in the nonfasted animals. The largest kidney observed, weighing 15.8 grams, was from a 341- day-old nonfasted rat fed this diet. Although large kidneys were a frequent finding in SPPB rats, the kidneys of a small group of older non- fasted rats that had lost less than 100 grams were normal in size. This group of animals included the oldest surviving rats on this diet. Enlarged kidneys were a frequent finding when weight loss exceeded 100 grams, regardless of diet, although there were relatively few large kidneys among the older SPM rats. The relation of kidney size to the extent and type of damage observed was similar to that previously discussed for rats fed SP 8 HVO or SPE diets; hyalin casts were gen- erally present in kidneys weighing 2 grams or more and cystic and glomerular damage in those exceed- ing 3 grams in weight. The livers from SPPB-fed rats tended to be large but were generally smaller than those from SPH- fed rats. In contrast to the results with SPE diet, the large livers from rats fed SPPB diet generally showed little microscopic evidence of fat. Rats FED SPE DIET WITH ADDED NUTRIENTS.— In table 31 are summarized data for the kidney and liver weights of rats fed the 10 supplemented SPE diets. Enlarged kidneys and livers such as TaBLE 29.—Kidney and liver weights of fasted and nonfasted rats maintaining weight at different ages on diets with protein-fat-containing foods Aver- Kidney weight Liver weight Aver- | Kidney| Liver | Liver Condition, age of rats Rats age age to to to (days), and diet age body | body | body | kidney Average Range |Average| Range weight | weight | weight | weight Fasted rats 200 to 399 days: Number| Days | Grams Grams Grams Grams Grams | Percent| Percent| Ratio SPISCHViOmene nek 5 313 1.37 | 1. 23-1. 60 10.7 | 9. 1-13. 1 523" 0 0427 al hs fell Bal end a his ScD Sea peters 5 313 2.09 | 1. 60-2. 91 21.7 | 17. 0-30. 1 578 . 36 ay ff 10. 6 SiIRAVN a Bial aiccnrm fines 6 304 1.38 | 1. 06-1. 85 11.9 | 7. 3-16. 2 580 . 24 2. 0 8. 6 SRA Beene alee atin SS 6 304 1.52 | 1. 16-2. 23 11.9 8. 7-15. 5 540 . 28 Phe ete RSE 1 BEY Bel re 6 289 1.43 | 1. 12-1. 91 11.8 | 10. 4-14. 3 495 . 29 2, 4 8. 6 400 to 499 days: SP SHEViOseS toes. 10 523 2.01 | 1. 43-2. 59 14.6 | 11. 9-18. 2 716 . 28 2. 0 7.4 SP hima tesart awh os 6 496 | 2.09 | 1. 55-3. 82 18.9 | 14. 3-24. 4 648 AS2 2.9 9. 9 PHI PAIK Crees an ea a 7 503 1.90 | 1. 438-3. 20 13. 8 | 10. 4-19. 6 659 . 29 2. Tes SAB ee apoee ee cathe es 8 8 502 2.16 | 1. 33-5. 57 13. 8 9. 4-18. 8 642 . 34 a. I 6. 6 SIPIAB cremains aot 8 509 2.06 | 1. 46-3. 68 16.9 | 13. 0-22. 5 741 . 28 Das 8. 5 Nonfasted rats 150 to 250 days: SP 8 HVO 10 201 2.08 | 1. 43-3. 32 21.6 | 14. 3-30. 7 530 . 39 4.1 10. 5 10 201 2.04 | 1. 76-2. 22 23.9 | 19. 2-27. 1 540 23o 4.4 11.8 10 202 1.98 | 1. 51-2. 34 21.2 | 16. 5-27. 5 581 . 34 Sy ie LOST, 10 202 1.90 | 1. 55-2. 24 18.6 | 16. 5-21. 9 540 . 35 3. 4 9.8 10 202 1.96 | 1. 59-2. 85 | 20.0 | 15. 0-27. 7 569 . 34 3.5 10. 3 TABLE 30.—Kidney and liver weights of fasted and nonfasted rats losing weight at different ages on SPM, SPB, and SPPB diets Kidney weight Liver weight ! Condition, weight loss, age (days), and Rats Average diet age Average Range Average Range Fasted Losing less than 100 grams: 200 to 499 days old: Number Days Grams Grams Grams Grams SPM a2. Sc 5 350 1. 63 | 0. 99- 2. 60 13.4 (4) 7.7 -17.2 pgp pte eae ee ie ee 3 268 1.22 . 85- 1. 47 9.9 7.3 -11.9 Nol 2a oe & fees eee eee em ty ee eae eee 17 330 2. 26 . 56- 7. 41 15. 2 5.0 -21.5 500 days and older: lS Deel of a ee = 5 528 2.50 | 1. 93- 4. 35 16.2 14.7 -18.1 DE Bie ete eee eee ete 4 651 2.52 | 1. 68- 4.53 15. 6 12.7 -20. 2 SPPBe 2225. cece cect enlace 5 586 3. 25 | 1. 38- 7. 09 18.2 11.0 —24. 7 Losing more than 100 grams: 200 to 499 days old: Db Mi 222i eee sues 1 362 1.10} 1.10 9. 63 9. 6 ii og 2 anne Re ON ciee aha ne eee i 461 fees ae 12.5 12. 5 DEP Ben 28a ec eee aoe e Se ae [teas a eee at a ec a | 500 days and older: PM m2 oS Soke oe sete cee 3 796 297° | 1e63=25. 53. 13.0 10.1 —15. 6 DP Be Sie ae ee tee See 4 630 2.82 | 1.50—- 3. 48 12.2 11.0 -14.8 DEPP Bs 2222 son ceneweseaeecel 4 531 3.80 | 2.12- 5.78 19.4 (38)} 12.5 -28.8 Nonfasted Losing less than 100 grams: 200 to 499 days old: PV Si DE eae ene Sh 7 354 2.67 | 1. 30- 6. 28 18:2 14, 7 -22.3 No fb Rane ee eee ee eee CR oa I 7 312 2. 05 . 96- 3. 70 16.5 (6) 8.7 -27.7 SERB ec leaneee tee es 6 373 5. 50 | 1. 52-15. 8 22. 5 9.8 -33. 1 500 days and older: DIME 222 oo eee ce ae 2 728 2.23. |) 1.83). 2.63 19. 4 18.0, 20.8 SRDS 22 sec cooceecce sees 3 696 2.53 | 1. 64— 3. 36 22. 8 15, 231.3 NP PBe i522 eede eben ee eee 6 643 1.98 | 1. 77- 2. 28 19. 4 15.2 -25. 8 Losing more than 100 grams 200 to 499 days old: PIV fae he eh See tesa 5 433 4,46 | 1. 48- 8. 68 16.5 12.0 -21.8 Rs tos ooh ee ee 5 435 4.93 | 3. 01- 6. 40 16.0 13.9 -18. 1 PIP Bis e528 ae one Sie ec ee 5 397 6. 06 | 3. 84- 7.99 22. 2 11. 8 —29, 2 500 days and older: Vie Ae oe cee ese t 706 2.94 | 1.58- 4. 41 15.1 (5)| 11.4 -18.3 Mii oase tt ee roses caeesee 9 609 5.08 | 2. 00- 8. 03 16. 3 13. 4 -21. 4 RS erat ae eee te 4 606 5. 20 | 3. 89- 6. 25 18. 4 14,2 -21.9 1 Numbers in parentheses indicate when number of livers do not correspond to number of kidneys. have generally been observed in rats fed SPE diet were also obtained with all of the supplemented diets. The kidneys from rats fed the diets supple- mented with vitamin B, tended to be smaller, on the average, than those from rats fed the unsup- plemented diet, paralleling the histological find- ings. The largest average kidney weights were obtained when the diets were supplemented with vitamin By. or with cholesterol. Although these supplements were without effect on the lifespan of these animals, there is some indication that they may have influenced the metabolic processes in- volved in the utilization of these diets. Consider- ing the wide range of values obtained in moribund rats, however, data uncomplicated by excessive weight loss are needed to establish the significance of the trends observed. RATS FED DIETS CONTAINING 8 OR 16 PERCENT HVO, tarp, oR BUTTER.—The kidney and liver 46 weights for rats fed diets in which the kind and/or level of fat was varied are summarized in table 32. Data from animals that were maintaining weight are limited to a group of five nonfasted rats fed SP 8 HVO diet and the corresponding results with the littermates fed SP 8 lard. No significant differences in the liver or kidney weights of these young rats were obtained. The results for sick or moribund rats have been separated on the basis of weight loss before sacrifice. When weight loss was less than 100 grams, the kidneys from rats fed diets containing hydrogenated vegetable oil at either the 8- or 16-percent level tended to be smaller than those from rats fed diets containing 8 or 16 percent lard or butter. When weight loss exceeded 100 grams, the results for fasted and for nonfasted rats have been combined because of the small differences usually observed in these two groups of animals. The kidneys from this latter 0 LE-F FI | 8 ‘0G €8T VET 6 LL -LEG G 66-8 “CI | 3 ‘6T OST 967 8 v OL-0F @ 9 °SE-L ST | G2 al SOF OT 8 GI-L0 % G "€e-9 “LT | 8 GS EST SSP 6 0 ‘TI-9¢ ‘% Py “S¢-9 ‘OT | 6 6I 6&§T O87 OT 6 OL-1L % v ‘9G-2L ET | 9 61 SEI (any 6 8°L -69 'T 6 ‘96-0 ‘9T | € 0G 9GT GLP 8 G GI-ZGI @ 0 ‘EF—-0 ‘OT | 6 FG FIL TEP OT L GI-0F % L°L6-8 “ST | 0 'TG Té1 GOP 8 T “ST-66 @ 8 ‘O&-S FI | 6 02 aa LSP 8T 6 ‘OI-06 'T 6 GE-E FL | GTS al SSP 9T 9 ‘TI-82 T SWDID supp | sun shogq | saqunyy SUDLD asuBy | oseIOAY osuBy SSOT ase qyslem |osvlaay | syey QYSIOM IOAVT WSBIOAY spuarunu payfiind pappo Gururpjuos yaip Tdy wo 1ybrvam burso) syou fo sqybran waay pun haupry— Te ATA, OF ¢ Egg €8 ‘9 LVL 91 °S tL YP €¢ 'P ST 2 €L 9 9T 9 99 SUWDLED dSBIOAYV 4ysIom AOUPIST OLT €é1 (Gal €ST 6ET 8cI O Bee ood 19 6. 90 82. 5 85. 2 8.5 6.1 1027 102 72 10.0 and! over. =.2-5- 2 Sees 6 i a 82. 7 87.0 Seti Das: 1675 153 96 64 GY ai) 669 Vaz lic T’¢ 6. LE=6:6 Ze OT Fy S8-T ‘92 T G8 6 G8 OLS T9¢ | | rags ek ine ddd8 gE 69 962 Taig Sa a 8 ‘GI-G '€ 9°9 ¢ “L8-9 G8 G8 G G8 08 °¢ 669 cheese ee peer eros rr dds 61 6¢ Ge Go Ger G c'T GeLl=l 6 9 FI T 98-T “82 € ‘08 6 “G8 69% gE9 ea | Ee ce earns ners a Wds :SUIVIS OOT UBY} V10UL SUISO'T €& 68 90¢ EeSeG av. GT T “€6-G 6 6 ET € G8-6 TL 997 Q Lh 06 € STP Ose ar | sepia eae Se adds GG SY 69€ 8 °S-0 7 v'? 6 FI-0 2 @ OT 1 ‘O8-§ “GL G LL € LL 9T % TGV Oty 2a aoe 4 ote aged See aie dds GG OL VrV 6 77 E vv L ¥I-0°8 0 T & 148-0 TL S Lz 8 82 GL S8P OLA 51 |e ays ees Fe eee Wds :SULBIS QOT UBY} SST SUISO'T 6T £9 OGE € 9-1 7% & 7 E-81=8 aif ¢ TI 8 “28-0 “22 ¢ 08 GEL, 86 T EGG Cet See en 669 °F OOF 8 8V GLE GS-0 G GG 0 ‘91-6 ‘TI 6 ET 2 “T8-0 82 F 08 6 LL 67 T G66 | Seen Soe nal 668 °F 00% ‘ddds IG 89 O8E GS-8 E ov G ‘02-0 ‘OT Soa T ‘P8-T “82 8 ‘62 8 LL GEG 867 OSes nae See ee 66¢ °F OOF ST 0g O8% 6 9-Z 'E S 7 6 LI-8 TT € VI & 18-6 ‘82 0°08 8 92 6G T SOE Oe Sse cr ae se 668 0F 006 £ ‘dd LT €¢ Tés g‘o-T SE cw 6 FI-€ ‘OT 0 ET © G8=poLZ 108 BOLL 18 T 86P ES AA os Paneeentl Shtcat 66 94 OOF €I eV €9G T 9-9 % 07 8 “STI-T ‘OL G €T & I8-F 82 T 08 T ‘92 LET FOE O beers lie epmpauie ayer 66€ °F 00% ‘by | ‘bn bY JUIIIag | jUaolagd quaola gq qU9ILIT QUAIL g UII |JUIIIg | SWDLD | Shog aq -INdS -UNN [VUSIOM SULUTGYUIVYL ule} 93U 93v ase usy | 3%q -O1L] asuUvyYy -I0AW asuey -IdAV asuvy -IDAW {USIOM Aouply | 5B (skvp) syei Jo UsV YRiy uloJOLg 1048 A 3B 95R SUEDE f ase pur ‘orp ‘snzvys IYSIOM 4u97U0D [RIO T, 4ysIom AIp uO poseg “LOAY -LIAY S19 TddS Pv» ‘TdS ‘Wd Vo 1ybram burso7 40 Buvurvpjyurpwu _syou Worf shaupry ur yso pun ‘yof ‘ur1aj01g—' EG AIAV I, 65 TaBLE 54.—Protein, fat, and ash in kidneys from rats fed other experimental diets Aver- | Aver- Based on dry weight Total content Aver-| age age ; | Strain and diet Rats | age |weight\kidney|Water age loss |weight Pro- | Fat | Ash | Pro- | Fat | Ash tein tein BHE rats Num- Per- | Per- | Per- | Per- SPE supplemented with— ber | Days |Grams|Grams| cent | cent | cent | cent | Mg. | Mg. Mg. Choline; 0:5 95222522 eee oe 19 | 492 139 | 6.33 | 82.1 | 85.4] 90] 5.9] 950 95 67 By, 0.01 mg./100 gm_______---_- 8 | 463 181 | 7.11 | 81.8) 788] 96] 52] 954] 105 69 Choline, 0.5%+By, 0.01 mg./ POOQC Si Bo: 2 eee ee 10) 4384 | 114] 7.18 |} 82.3} 825)103)] 58] 963 112 71 ‘Bg, .0'o me: /LOOiem=. 22. = 22 ee 7 | 502 129 | 5.13 | 79.5] 820] 93] 56] 824 89 58 Choline, 0.5 %+ Be, 0.6mg./100gm 9 412 127 | 4.74 | 80.5 | 82.8 | 10.2 5. 4 724 80 49 Choline, 0.5%+ By, 0.01 mg./100 em.-+ Bz, 0.5 mg./100 gm______ 10 | 431 | 129 | 5.76 | 81.4|/808]103] 66] 896] 110 72 Cholesterol, 0.46%___________-- 8 451 150 | 7.54 | 81.5 | 83.2 | 11.0 5. 1 |1, 240 155 76 Cholesterol, 1.88% ___..-_____-- 10 409 142 | 6.83 | 81.6 | 85.3 | 10.1 6.8 971 109 78 Ascorbic acid, 0.2%_____------- 9| 415 115 | 5.00 | 80.1 | 83.0] 8&6] 5.2] 802 76 50 Ascorbie acid, 0.2%-+choles- arly O46) eet 9 434 183 | 5.74 | 80.2 | 85.1 9.9 6. 0 940 109 67 DE 16s Vi Se oe 8 629 84 | 2.76 | 77.9 | 78.9 | 18.4 4.7 446 71 26 Db Sard ses cose ste 11 557 99 | 3.26 | 79.5 | 82.2 | 11.6 4.2 540 7A 26 Seal 6derd: 2 ee. _..@ 2... ee 9 572 LI7 1:3. 31. | 79.7% | :82,2 | 13.2 4.7 529 81 30 SES DUbbeE enc 4i.. eee eee 8 | 601 82 | 2.58 | 79.3 | 80.5 | 12.4) 40] 400 59 20 Se 1G Diernca Sauer 9 486 72. | 2. 74°\ (8:0 | 7953.) 13.5 4.8 459 69 29 12210) | ea eee ee 9 | 559 141 | 4.36 | 82.9 | 82.7] 13.6 | 5.6 | 523 86 35 5 | ean ee eae eee ar 14 | 375 80 | 2.42 | 79.4 | 79.5 | 13.7 | 62] 363 61 31 Littermates fed— 1 el 3) ee le i er es alk em I prem 5 428 66 | 3.88 | 79.5 | 83.5 9.9 6.5 637 70 54 NOOR. a eae ee 4 420 42 | 2.54 | 80.2 | 79.4 | 146 6. 0 383 69 29 Y97-+salt mixture, 3.0%-________- 4} 424 6 | 1.66 | 77.8 | 80.8] 13.5] 5.71] 299 50 21 Diet reversal: Sacrificed at approx. 250 days: DbOGWe cn. oes a eee eee 3 | 250 0 | 2.07 | 788 | 79.8] 13.2] 3.0] 3841 56 13 Eee ee oe eee ee 3 249 0 | 2. 74 | $1.3 | 76.8 | 12.8 2.0 375 61 10 Continued on— DGG s ae Sank ee ee 3 | 590 £95") 4525 Sle Sneeeed | 58-15 ee oee™ 654 63) |2aeee2 Bias eee ee oe eee 2! 396 133 | 464 | 84.5] 788] 9381] 591] 565 67 41 Reversed at 250 days: Stock changed to SPE__________- 4 686 109 | 3.94 | 80.9 | 842 9. 2 4.9 624 67 35 SPE changed to stock__________-_ 3 STE 116 | 3.55 | 80.6 | 82.4 9.5 3.8 560 63 26 Wistar rats DE HEY Cheer yet Se 8 762 119 | 2.02 | 80.0 | 82.0 | 13.5 4.3 323 54 18 to] Edo eee ae on ee ee 5 772 103 | 2.80 | 79.6 | 80.9] 164] 43] 446 89 23 kidney and a high percentage of ash did not are grouped by age at which urine collection was necessarily parallel one another. Differences between the composition of kidneys from BHE and Wistar rats were due chiefly to differences in the kidney weights of these two strains of animals. Urinary protein BHE rats.—Data on urinary protein excretion were obtained for BHE rats fed 11 of the diets under investigation, and the results are sum- marized in table 55. Urine was collected under two conditions: (1) a 7-hour collection period without access to food; (2) a 17-hour collection period with access to food. The conditions of collection are indicated in the table, and the urinary protein values recorded are for total protein excreted during the collection period. Results 66 made; in most cases the age range within groups was less than 2 weeks. Data for age at death and size of kidney are included to relate as well as possible the protein excretion with age at death, although urine samples were not collected at this time. The amount of protein excreted by rats under 200 days of age was generally small regardless of diet. When urine collections were made for a 7-hour period without access to food, protein excretion was less than 10 mg. for 67 percent of the rats. Only 3 of the 61 rats in this group excreted more than 50 mg. of protein. When data obtained under comparable con- ditions were available, a tendency for increased protein excretion with increasing age was apparent. Dietary differences were observed in the older “OATIB [[I]S JI JBI OUTRS OY} IOJ 919M JOIP YOVa WO SdnoIs 93B OA} OY} IOJ VIVE ¢ *S]BI POSRJMOU WO] SINOY LI IOJ ‘s1VA paysvy WIOIJ sIMOY JL OJ poyo[[0o oul 1 0 -----2----]----------] 9 23°% FOL z 81% 9F8 b ener non| Seeeaeead 0 a oa 80°E SES z 36 'T Fg8 b pepe Pmen- aes 0 68'€ LOL I Il‘? LPL ion Pt, (een cenernee) |Iaeeaaana 0 a be Saeee | ieaeasnacninan | Seana 0 #0 Pel in | caciaieiie | 0 SSokeeniae | erent 5/10 Lb Geo I 69 '€ Leh SSI a fe ears a| er ocoie ng | 9 0 LP oes T SIF 104 T SF's gg. z ae Rete silt sos cal 0 G3 629 | nn CaS ech | Seana 0 Eee; | encanta | nena 0 wv 968 z 2'e 629 TE | cama | Gaceaama Q000 [reeecesene|ecessesee a 50" Ozh I 988 S19 AS clasps katy cer iy ee Melee Soe 0 40'S OzF I 96 'F LOL if 9L°% £29 Te. 4 Ulaetesan or | ees 0 86°F RoE To, Ore "| eao | aeeerall 0 6 F ggg g 0S 'F &19 4 S05E Tres |2 00% I 00°2 399 g ge'e 869 é ze 699 I ojos 0 = tie 1-aai lite ee EO) an 0z¢ z 19° ggg 8 +---------|---------- 0 8o°¢ 00¢ Zz 709 (aG7 z 89g alg 9 ~-+-------|---------- 0 scomsncied pareemoncel| 09°6 LOP z 16 °€ Te9 8 ed ee 0 ze'8 Ieb I #901 ehP g 63°9 arg 9 ieee | aera 1) 86 €19 T oF TD 119 P G6 808 g 0 c6°L GPS I 16 € 06¢ z 16 °E ELE, L 19 °¢ oa CS in Geico, |B canes 0 oe'€ $29 9 02% 129 z Ig ‘F OSF T CPG org F PL'G 88¢ 9 go's GOL g 8 °F Por C= Wiel | Pc cee || ee aioe 0 86% O19 g (age PL9 L 68 ‘TT FIP a 60°2 O6F € &% 9 og¢ 4 68 °L 609 z S850) oral: Shr. 8 69 II Ch I (ans 629 if 588 08¢ I OR sent |imipecamere |nanaa nian 0 ¥9'°E 862 8 Sh SG 608 1 LG aieaetoe || Leone I 68% 802 z GL 's £19 L 18:8 969 g ; ONS ee re as | aps cae a 0 8L°E agg g 16° OFL L Obs oe | OFS aa) I 18 € 688 | es | ee eared beer Oi yo Be ea 0 0 661 CGF I 8g £29 z 29'S POL L peer | tae os. | 0 be eeeb tes | ha ae So ao | areciew Ons el pig oie hale ee z Oates | eit eas ca | ae an ge Teh os cee lig One ek Rae G) fa ee Dea en | aes 9 SWUDL}) shog Laqunn | smDip shog daqunn | swDpip shog waqunn | swpsp shog LaQUenny WY sTOM yyvep qUSsIom yyeop qy3IOM yyeep 4ystom yyeop Aoupry | yeosy SJB Aoupry | 7B 0sV syey Aoupty | 38 08V $78 y Aoupty, | 4v 03 V Sqey JOAO PUB “BUI ODT “SUL 66 01 0G “BUI GF 01 OL “SUL OT URYY Ssa’T —JO WOTJOIOXO UIO}OIg 02-0 6 68-0 IT #9-$E cy LI@-LIT 89 F6-08 0g TL-¢ 1 6¢ 6g €II-$9 c6 883-88 OFT 80G-F3 lane SZI-9 ¥G IST-@ 9g IIl-@ 9 S6-0 FG SI-1 9 gs-% ST &S-F LI S8-T LI OIg-p og I9I-¥ (Gi €12-@ 62 ELE-S 961 IFI-9 18 La-% Or FPG FF It-F 6 02% ‘66 | O9T €8-T 6r 96-0 9¢ €L-% LT “ON ‘ON osuvy | osRI0Ay ul9}0.1d 9ULIN [8}0.L P99 #99 93B asBl0AV 9 1Z ta Or z Or g ai LaQuunny S}VY paras wes poqsBq ‘ads haa Re cie eam ae pose, :4004s 0} posueyo Wdsg isc a ara peyseT ‘Hd 0} posueyd yoo1g :sABDP 0GZ 1B PISIOADNY 2209S —u0 panuyjuod $7By T[BSIOAOI JIC Cee i dea. mia at pose ‘ddds a poqsejJuON ‘dds eGR Seay poqseJUON ‘Wds Poo pt saa poqsvjJuON :ads as ele Oe poJsejJaoON ee ae ae ee as ee pose y (OAH 8 ds ee ea iene po4se 230019 5781 GHE UWOT}09T[09 oUT.M JO} SUOT}IPUOD PUB “YyoTp ‘UTeIIg 1 SJ2Up SNOWDA UO Saby qualaffrip yp SDL payspfuou pun pajsvf fo uraqoud fiuwur1t4qQ—'sg AAV, 67 64——_6 721-631 rats. Of the nonfasting rats approximately 375 days old, those fed SPE diet excreted the most protein. Differences in excretion by rats fed SP 8 HVO, SPM, or SPB diets were small. Rats fed SPPB diet tended to excrete somewhat more protein, and the excretion of 3 of the 11 rats exceeded 100 mg. WISTAR RATS.—Protein excretion by Wistar rats was low on SP 8 HVO and SPE diets,in marked contrast to the results with BHE rats. For a 7-hour collection period, urinary protein aver- aged 9 mg. for fasted 614-day-old Wistar rats fed SPE diet. No data were available for a comparable age group of BHE rats because of their short survival on this diet. However, by approximately 350 days, BHE rats were excreting 87 mg. of protein when urine was collected under comparable conditions. URINARY PROTEIN AND KIDNEY DAMAGE.—Most measurements of urinary protein were made con- siderably before the age at which the animals died, and provide little direct evidence to relate extent of protein excretion to kidney damage or age of survival. The low urinary protein from rats less than 200 days old gave little indication of the extent of kidney damage at death or of the lifespan of the animals. The only young rat (180 days) to excrete over 100 mg. of protein had a 5-gram kidney when he died at the relatively early age of 358 days. In rats 350 to 400 days of age, urinary protein appeared to be a fairly good index of kidney damage and expected survival, with lfespan generally decreasmg as urinary protein increased. Discussion.—The results for BHE and Wistar rats reported in this publication confirm the find- ings of other investigators that some protein may be excreted by animals with kidneys that appear normal in all respects. Urinary protein for BHE rats was considerably greater than that generally reported by other investigators. Gilson (72 found proteinuria to be a usual occurrence in Wistar and Sprague-Dawley-Holtzman strains of rats. The average excretion during fasting was 3.0 mg. globulin and 3.3 mg. albumin in 24 hours. Particularly heavy protein precipitates were ob- served in the urine of a group of animals main- tained at a temperature of 4°C. for 3 months. Saxton and Kimball (168) reported appreciable excretion of albumin by rats over 300 days old. Proteinuria was found to increase with age, although there was somewhat reduced frequency of protein excretion in rats over 800 days old. McCay, Maynard, Sperling, and Osgood (121) obtained an average daily protein excretion of 23 mg. for rats on a low level of dietary protein and 82 mg. for those on a high level. Albumin was found in the urine of mature rats with normal kidneys, but with chronic nephrosis increased amounts were present. There appeared to be a rough correlation between kidney damage and increased protein excretion, although the lifespan of the animals did not appear to be related to the 68 latter. Rather (155) suggests that the thresh- old of the kidney to protem may be due to tubu- lar resorption with proteinuria occurring if tubular resorption capacity is exceeded. Using rabbit anti-rat kidney serum to produce nephrotic rats, Drabkin and Marsh (51) observed a marked increase in labeled urinary protein after injection of labeled glycine into the nephrotic animals. Total serum protein decreased and the albumin moiety almost completely disappeared. Summary.—Urinary protein excretion in BHE rats tended to increase with age and to be in- fluenced by diet. The extent of the proteinuria observed appeared to parallel the occurrence of degenerative changes in the kidneys of these animals. Protein excretion in the urine was con- siderably greater than has generally been observed and seemed to be related to the shorter lifespan of these rats. Protein excretion by Wistar rats was generally small and within the range reported by other investigators. Liver RATS MAINTAINING WEIGHT ON stock, SP 8 HVO, anv SPE pirers.—In table 56 are sum- marized data for protein, fat, and ash in livers of rats maintaining weight on stock, SP 8 HVO, or SPE diets, and included are results for per- centage composition and total content. Most of the results recorded are for fasted rats. Limited data were obtained for nonfasted stock rats 300 days of age and older and for a group of young rats less than 300 days old fed SPE diet. Age appeared to have little influence on the composition of livers from rats fed stock diet when the glycogen content of the livers from nonfasted rats was taken into consideration. Values of 76.2 percent for protein, 15.8 percent for fat, and 5.0 percent for ash were obtained when the results for nonfasted rats were calculated on a glycogen- free basis. The percentage of protein and of ash in the livers of rats fed SP 8 HVO diet did not differ with age, but the percentage of fat showed a con- sistent tendency to increase. With SPE diet, as with stock diet, the composition of the liver appeared to be uninfluenced by the age of the ani- mal. When the data for nonfasted rats fed this diet were calculated to a glycogen-free basis,’ values for protein, fat, and ash were 55.8, 34.1, and 3.9 percent, respectively—values differing greatly from those on the stock diet. Data comparing the content of the livers from fasted and nonfasted rats were limited but seemed to indicate that diet could influence the response of the liver to fasting. As the result of a 17-hour fasting period, the protein, fat, and ash content of the livers of rats fed SP 8 HVO diet were reduced. Both protein and ash content were smaller in the 8 Assuming a liver glycogen of 8 percent on the dry- weight basis using a value obtained for comparable rats (unpublished data). 96 ° 6y € | 624°8 Os a v's P GS-8 “OE T SP € "€9-8 “68 & '6P 9 “09 66° c6 7 | IL P L&-¥ @ TS € ‘€9-0 “66 8 '8P 9 ‘¥9-9 “SE GSP 02g TS * 0g FV | 88°F T P-G % as 1 ‘OG-T '8& 8 St V PG-8 “OP GLY ¢ 69 9g ° GEE | 93'S G ¥-0'E 9° ¥ Lb-E CLT VTE G 19-3 TV v Ig I ‘G9 GG * ge '€ | 49 € 0 'V-¥ % T's G “GS-0 “LE T 8? € ‘99-T “IP € 1g 9 6S Was 66 ° &I T ‘$-0 7 97 9 “8E-7 “LT 8 1G 8 EL-8 “9 6 ‘89 £19 OLS 68° | 46% L0-G 'V oT ¢ 'E¢-8 ‘FI 9 ‘6T 6 ‘PL-Z “19 LOL 9 89 81° €L° 62% T 9-2 7 97 ZIG-€ ‘ST 9 ‘8ST G 8L-¥F “19 9 GL L°L9 Ses 09° TG % Te Secey. 9° 9 ‘1é-G ST 6 LT 0 SL-T “L9 612 F 89 LE ° PS ° 0 F Lae Gov, Lv 9 ‘PI-Z FT € FI 6 ZL—-L “99 0 ‘OL 8 TL GG * €2° OL '€ 6 ‘-0'F vv 0 ‘8I-¥ ‘ET 6 ‘FI v GL-0 “69 GOL T ‘04 tT ‘0 | 89°0 |} 19% So F-SS a LZ '06-G ‘ET 6 ‘OT 8 08-8 “EL 9 ‘92 T 29 SUDLD |SUDLD | SWDLH | quaoiag |yuao1ag Ualag — |\juaowag JUIILI JUIDLAT |\JUIILIT ulo4 958 938 3B ysy | 38 -O1g osuByy -1OAVW asUvyYy -I9AV osUuRyy -I9AV USV ye] ulo}Olg Id4eM 4U9}U0D [BIO J, 4ysiom AIp uo poseg "S]BI poySBJUON 1 ARPOOD oS Nn 8g ESP EGE 926 CSG LUG LSP eG GGG O8¢ civ PGS shoqg bal Oro COOrN wPHroeo al LaQUnN 058 o5eB -IDAV SPBY Domine ake eoe 66¢ 04 00S Ge Silane mere 66F 03 OOF hae Patel teeeanet 66€ 07 00 Saran aetna 1002 Ue} SsorT he ak Aa 00g UBYY Sso'T ‘dds ee Sia Cn © eee 66¢ 94 00S (Pe mee ae od a ee 667 °F OOF Rect Se 66€ 97 OOE art am meranty 00 UeYy Sso'T ‘OAH 8 d$8 fo cya ae es , 1OAO pue QOS el Sas 1 66F 9F 006 Baa, sepes aa 00g UBYy sso'T :yoo1g (skep) Syer Jo ose pue JoId SjUp Ad S| PY” ‘OAH 8 dS ‘2048 uo sabv quasafjp yo yybram bururpyuroun spon WoL Sad UL YsD pup “yoH ‘ULd},0L—' 9G DAV], 69 livers of fasted animals fed SPE diet than in those of nonfasted rats, but the fat content re- mained approximately the same whether or not the rats were fasted prior to sacrifice. Although the percentage of liver fat among individual rats varied considerably, the livers from stock or SP 8 HVO rats were not considered fatty; those from SPE rats were consistently fatty. Only two of the animals fed SPE diet had livers with less than 30 percent fat. The highest per- centage of fat observed was 63.3 percent in a liver from a rat fed SPE diet. With the techniques used, fat was not detected microscopically unless present in excess of 30 percent. When the concentration of fat was be- tween 30 and 40 percent, numerous small vacuoles were observed. When fat exceeded 40 percent, large as well as small vacuoles were apparent. Although protein and ash in the livers from SPE rats were diluted by fat, the total content of each was equal to or greater ‘than that found for these components in the livers of stock or SP 8 HVO rats. In table 57 are summarized data on the composi- tion of livers from rats fed stock, SP 8 HVO, and SPE diets as related to liver weight. There appeared to be little evidence that the composition of this organ was influenced by its size. Livers from stock rats were similar in composition whether their average weight was 14.9 or 23.1 erams. The percentage of ‘Tat j in livers weighing less than 16 erams was low in comparison with the larger livers from rats fed SPE diet. However, small livers were rarely seen in rats fed this dict. The increase in liver fat with age that was ob- served in rats fed SP 8 HVO diet seemed to be related, in part at least, to the body weight of these animals. In table 58 are summarized the results for liver fat as related to body weight. For animals weighing less than 600 grams, the increase in the average percentage of fat with age was small and, considering the range of values observed, was of questionable sionificance. The percentage of fat in the livers of rats weighing between 600 and 700 grams was significantly higher (P < 0.01) than in the livers of the lighter animals. Of the 11 rats weighing between 600 and 700 grams, 10 had livers containing more than 20 percent fat. In the larger rats weighing more than 700 grams, there was some indication that liver fat was lower when kidneys showed evidence of damage. The 4 rats in this group with liver fat less than 20 percent had kidneys showing de- generative changes; the 2 rats with normal kidneys TaBie 57.—Protein, fat, and ash in livers of different weights from fasted and nonfasted rats fed stock, SP 8 HVO, and SPE diets Condition, diet, and range Rats of liver weights (grams) Nonfasted rats: Stock: Tress ethan 6.0) <2 a ee ee lee T6sOnton 1 Oi0 et ens ae ee 20.0 andover..-- 22-523 h eee ee Fasted rats: SP 8 HVO: Toess*than’ 12.0228 oe ee TOGO de 2 tee eee, ee ee ee i Le: O10 rte al Hs a, ° Rn end ae em eye yee L6:0 ‘and OVversel kes 6 eee See SPE: (béss: than: 16:02) saeco seek ees LOO) COehO. Oe es a ee ee ee 20:0 SG OVERE 225.6 oe ee ee a wocr a Number 4 wWnmw Average Based on dry weight liver Water weight Protein Fat Ash Grams Percent Percent Percent Percent 14.9 710 70. 4 15; 4. 4.3 16. 6 Ville 74 68. 0 14.3 4.5 2301 69. 9 69. 4 14.8 4.7 10. 7 67.9 72.0 19. 0 4.6 13. 0 68. 4 TAS: 18.1% 4. 6 14. 6 67. 4 68. 2 22. 4 4.6 18. 0 10:2 7022 18. 0 4.7 14.1 64. 6 64. 7 28.9 3.8 18. 2 60. 3 50. 9 42.6 oi 24.1 59. 0 48. 4 45. 6 ono TABLE 58.—Liver fat and body weight of fasted rats maintaining weight on SP 8 HVO diet Liver fat on dry- Body weight range (grams) 70 Rats Number 6 10 i 6 Average body weight Grams 462 550 653 742 Average weight basis liver weight Average Range Grams Percent Percent 10. 4 17.1 | 14 8-19. 1 12. 4 18.0 | 15. 5-21. 7 13. 7 22.1 | 19. 4-28. 6 14.6 21.0 | 17. 8-28. 3 had livers containing 24.9 and 28.3 percent fat. The number of rats with damaged kidneys that were maintaining their weight on this diet was small, and more data are needed to establish the possible significance of this relationship. On the stock diet, there was relatively small variation in body weight, and data for rats weigh- ing more than 600 grams were too limited to deter- mine whether or not there was any relationship between body weight and liver fat. On SPE diet, with high concentrations of fat in the liver the usual finding, there was no evidence that the percentage of fat in the liver was related to body weight. A rat weighing 783 grams had a liver containing 38.6 percent fat; one weighing 530 grams had a liver containing 51.2 percent fat. Rats LOSING WEIGHT ON stock, SP 8 HVO, anp SPE prers.—In table 59 are summarized data for rats that were losing weight on stock, SP 8 HVO, and SPE diets. The results are reported for non- fasted and fasted rats with further separation on the basis of the extent of weight loss even though the number of animals in some groups is small. Most of the results with the stock diet were for nonfasted rats and were similar to those observed with animals maintaining weight on this diet. When weight loss of nonfasted rats was less than 100 grams, the influence of glycogen on the per- centage composition of the livers was apparent. Regardless of the extent of weight loss of fasted moribund rats fed SP 8 HVO diet, the composition of the liver was similar to that obtained for fasted rats that were maintaining weight. In nonfasted rats fed this diet, livers tended to contain a higher percentage of fat than in the fasted animals, but again there was little evidence that extent of weight loss influenced appreciably the composi- tion of the livers. In contrast, the extent of weight loss before sacrifice for rats fed SPE diet seemed to influenced liver composition more than fasting. The fatty livers characteristic of rats that were maintaining weight on SPE diet were seen in many of the moribund rats fed this diet, although the percentage of fat was generally somewhat lower than in rats maintaining weight. When weight loss exceeded 100 grams, there was a marked decrease in the number of rats with liver fat exceeding 30 percent. The wide variation in liver fat of nonfasted rats that were losing weight on SP 8 HVO diet did not appear to be merely a reflection of reduced food intake and extent of weight loss before sacrifice. Low liver fat in these rats seemed to be associated with excessively damaged kidneys, whether or not there had been appreciable weight loss. Data for liver fat and kidney damage are summarized in table 60. In the first group are included values for liver fat when kidney damage did not exceed a rating of 2 for hyalin casts, with no glomerular or cystic damage. In the second group are the results for rats with kidneys showing cystic and glomerular damage as well as hyalin. Of the 8 rats with kidneys showing little evidence of dam- age, 6 had livers containing more than 26 percent fat; only 1 of the 7 rats with extensive kidney damage had a liver containing more than 20 per- cent fat (21.5 percent). A similar trend has already been discussed for fasted SP 8 HVO rats that were maintaining weight. On SPE diet, high liver fats were found consistently in rats with kidneys showing little or no kidney damage; the highest fat was observed in a rat with small normal kidneys. However, high liver fats were also ob- tained frequently in rats with extensively damaged kidneys, and no consistent relation between fat in the liver and kidney damage was observed on this diet. RATS MAINTAINING WEIGHT ON SPM, SPB, ann SPPB prers.—In table 61 are summarized the more limited data for the composition of livers from rats fed SPM, SPB, and SPPB diets. Data are presented for two age groups: those 200 to 399 days, and those 400 to 599 days old. No marked differences were observed in the composi- tion of the livers of the young rats fed these three diets, and the results are similar to those already reported for comparable animals fed SP 8 HVO diet. In the older rats, the percentage of fat in the livers was higher and the percentage of protein correspondingly lower than in 200- to 399-day-old animals. Liver fat for rats fed SPB diet was similar to that observed for comparable rats fed SP 8 HVO diet. Higher liver fats were obtained, however, for rats fed SPM or SPPB diets. The percentage of ash in these livers was similar to that found in the livers of SP 8 HVO rats, and no differences with age were observed. The high liver fats for rats fed SPM and SPPB diets appear to be related to their body weight. Differences in body weight, however, do not explain the results for rats fed SPB diet, as seen in table 62. On the diet containing milk (SPM), liver fat increased on the average from 16.9 for rats weighing less than 500 grams to 29.7 for those weighing 700 grams and more. A similar trend was seen with rats fed the diet containing peanut butter (SPPB). On beef (SPB), however, only 3 of the 13 rats had livers containing more than 20 percent fat, with one of the highest values observed in a rat weighing 486 grams. RATS LOSING WEIGHT ON SPM, SPB, anp SPPB piets.—No consistent trend with age was apparent from the data on the composition of the livers from rats that were losing weight on SPM, SPB, or SPPB diets. Therefore, the data in table 63 are the results for all age groups combined. The livers from these moribund rats showed differences in composition that were similar to those from rats that were maintaining weight. Liver fat was consistently low for SPB rats, both fasted and nonfasted. Liver fat tended to be high in SPM rats, and again the high fat values seemed to be related to the large number of heavy animals on this diet. The highest liver fat with SPM diet was 47 percent in a rat that lived to be 799 days old and reached a maximum weight of 1,020 grams. vA NOD NOH mN aN 6G ° 6h S| GE 6 ¥-0 0 ?T L8S-€ “ST 6 66 IZ ° 60 T | ves 8 PFE & 'v 9 ‘LE-6 TI 9 TS 96 ° tL 66 6 y'S-L PV E'S 9 °ST-9 ‘SI G ‘FI 96 * 64 F | 918 8 E-€ % € 0 09-T ‘8E SP GS ZEW AeS Sis LvV's Tv 9 ‘9Z-G “LT 8 1G 0s * 16° G9 P GT's? v? T PTL OI 9 ST 62° 8L'T | ol? o S-0 7 9? 6 ‘0G-G ‘FI € 9G GS * GL° GSE 87-17 87 9 ‘9I-L “ST GOT &Z * LYS | 99'S 1 G-L 1 8 'E § ‘09-8 “ST 8 TE Le g9° GGG & G-9 'P 4 ¢ ‘0c-T ‘FI 6 ‘LT 8T 0 c9 ‘0 | 89% ES is 8 LT 8 CLT SUDID |suDID | SDI | JUuaIIag UIILIT qUIILIT JUIDLIT ulo4 ose sic) Usy qe -Olg osuevyy -I9AV oSUvyY -10AV Usy Ve 4U94U09 [BIO J, “GL-P GP ‘6Z-T “LS ‘08-9 “GL ‘VS-T “EE "GL-8 LG 69 ‘2 “89 ‘T8-Z GE "EL-@ 69 PL-G GE ‘LL-6 ‘99 GcLL quaa1ad WUIaII | WUawWag NOOO HHA uw “LO ‘69 ¥ S9 9 69 9 ‘OL AY G 8 I g9L I 969 G 966 i P8E G Pgs NOD WHO ¢ ‘61 CGP 8 0ST 0sg € ¢cLT 168 8T LTT TOG 9 8 TT LGL T suDiy | sing |saqunay dsUByy uloyolg yyustiom Ap uo poseg o8eB -10AW TOE M VUSIOM IOATT 9B a08 ase SJVYy -I9AW | -IoAV Tie ee OAH 8 d$ 3048 ‘SUIBIS QOL UBY} A1OUL SuISO'T 9048 ‘SUIVIS OO] UYY SSe] Sutso'y S}V1 poysvjuon HOS :SUIBIS NOT UBYY SSeT SuIso'T s}ei poysey 4oIp pue ‘ssoT VYSIOM ‘uOTIpUOD Sap Ads PUP ‘OAH 8 dS ‘2038 Uo yybram Burso] syou payspfuow pup pajyspf wodf sua) Ur ysp pup Gof ‘ur9j01g—"6S AAV, 72 Tas LE 60.—Kidney damage and liver fat in nonfasted rats losing weight on SP 8 HVO diet Kidney damage o 2.0 hyalin, no cystic or glomerular damage_______- ~~ Ot 2.0 hyalin or more, with cystic and glomerular damage___-__-_ 1 Based on maximum rating of 4. Liver fats tended to be high in nonfasted SPPB rats. Rats FED OTHER EXPERIMENTAL DIETS.—In table 64 are summarized the data available on the composition of livers from rats fed some of the other experimental diets. The results are chiefly for moribund rats, and interpretation is com- plicated by the variable weight loss of these animals before death. In spite of the wide range of values obtained, some differences were observed that seemed to be related to diet. On the various diets that consisted of the SPE diet with purified supplements, liver fats were generally high and similar to the results with the unsupplemented diet. When the diet contained cholesterol as the supplement, however, liver fat tended to be some- what higher than for the other modifications of SPE diet, in agreement with the results already discussed for microscopic examinations of this organ for fat. In the series to determine the influence of kind and/or level of fat, the livers from rats fed SP 16 HVO tended to contain a higher percentage of fat than those from rats on SP 8 HVO. Livers were not excessively fatty with any of these diets, and no consistent differences were observed that related to the level or kind of dietary fat. When diets of 100 percent whole egg or egg yolk were fed, liver fats tended to be low and protein correspondingly high in comparison with the liver fat of rats fed a diet containing 25 percent whole egg. The results for rats fed 100 percent whole egg and 100 percent egg yolk were similar to each other. The results for a small group of littermates showing little or no weight loss on SPE, Y100, and Y97-+ salt diets confirmed the finding that liver fat was lower with 100 percent egg yolk than with SPE diet. Supplementation of the 100 percent egg yolk with salt mixture was without influence on liver fat. The livers of Wistar rats fed SP 8 HVO diet were generally low in fat; 18.4 percent was the highest value obtained. The only high liver fat (42 percent) observed in Wistar rats was for a rat 874 days old that reached a maximum weight of 970 grams on SPE diet. Discusston.—Liver fats have been reported to be susceptible to many factors, including age, heredity, and diet. Much of the research dealing with liver lipids has been done on relatively young animals, and little information is available Rating for kidney damage ! Liver fat on Rats dry-weight basis Hyalin Cystic Glomerular Number Score Score Score Percent 8 0. 4 0 25. 6 7 3.0 2.4 a3 18.5 on the liver lipids of the rat throughout life. Andrew, Shock, Barrows, and Yiengst (/1) reported the results of histological examination of the livers from stock animals 1 and 2 years of age. The two groups of rats were very much alike. Vacuolation indicating fat storage was observed occasionally, but no consistent change with age was apparent. Periportal infiltration of lympho- cytes was seen in the connective tissue around the bile ducts, portal vein, and hepatic artery in some of the older rats. From chemical analysis, Yiengst, Barrows, and Shock (191) reported no differences in fat content of the livers from these two groups of rats. Grunt, Berry, and Knisely (80), and Grunt and Knisely (S/) reported that old animals have more hepatic fat than have the young ones, and that genetic factors appear to play a significant role in the development of fatty livers in the rat. Literature dealing with the many dietary factors that may produce fatty livers has been exten- sively reviewed by Deuel (47). Publications on this subject are numerous, and only a few that seem closely related to the results under consideration in this bulletin will be discussed. Blatherwick, Medlar, Bradshaw, and others (31) fed rats diets containing 75 percent dried liver for periods of 30 and 60 days, and obtained livers of high fat and cholesterol content. Fat accumulation was well marked by 28 days. Fractions from liver were fed alone or with various supplements, including lecithin and cholesterol. Marked differences in the fat and cholesterol content of the livers were noted, but changes in liver fat were not necessarily paralleled by changes in liver cholesterol. Cholesterol, when added to a diet containing a 70 percent alcoholic precipitate of an aqueous extract of liver, resulted in livers of extremely high fat and cholesterol content. Feeding certain fractions containing cholesterol, however, had relatively little effect. The feeding of cooked egg yolks produced livers with increased cholesterol content and, to a less degree, increased fat. More marked increases were observed with cooked whole eggs. McCay, Maynard, Sperling, and Osgood (121) reported higher total lipids, cholesterol, and phospholipids in the liver of rats fed dried liver than in those fed milk proteins. The lipid com- position of the livers of these animals was about 13 &Z° OV 'T | 89 '€ LV SS || Er | 9 66—G ‘0G | 6 FZ § ‘TZ—@ ‘9 | € 99 PLO GLI GGG TE ili eee Ae ek eee 66¢ °F OOF cI° Gis 88% So Bee | AOE v GC—0 ST | 8 '8T 0 °8Z2—8 19 | 9 EL 8°19 6 GI G63 Cee ee ee ne aa 66€ 94 00Z ‘adds 0% * €8° 90-8 G’c—07|3 7 8 '9Z—8 ‘OT | ¢ ‘0G GoVZ—v 99) | 2. 0% 6 89 6 “ET 60S (RE (a es a ea 669 93 OOF cT° 149° tL @ G ‘YS '€ | oP L'0@—6 ‘ST | 0°81 6 GZ—9 TL | V PL 6 ‘89 8 IT GOS 1S DY (aie ct es aa 3 66€ 9F 00Z ‘dds 61° 6T ‘I | 80°E GtT—9E | CF G EE—8 ST | 9 FG 8 GL—Z “GG | “19 Z ‘99 6 “ST v0g Se) Nits Biome sere 66¢ °F OOP 110 8Z°0 | 98% OL Vi -Se¥ T ‘96—L ‘9T | 6 61 € ‘92—Z 19 | 8 GL 8 19 6 GL TOS Otel ae = 5 Net aaa re 66€ 97 00G SUDLD |SUDI | SUDL | Juaag | JuaaLag JUIILI JUIILAT JUIILIT quaaag |jUIIag | supiy | svg |saqunyy -INdg ulo} o8B ase 5B sy Very -Olg osuBy -IdAY osuey -IOAY osuey -IdAV VysIOM i IOAI] 058 ysy 487 UuldJOI 10ye Bye o0e syey (sdep) syvl Jo ose pue yoiq -I0AVy | -IaAV 4uo4yUod [BIO], qyystom AIp uo poseg. sp TddS' PUP ‘TdS ‘NdS Vo ry bvan Gurwpyurput syou payspf wouf suaar) ur ysp pup “yf ‘urdj04g—" [9 HTAV J, 74 TaBLe 62.—Liwer fat in rats of different body weight maintaining weight on SPM, SPB, and SPPB diets Diet and body weight Average | Liver fat (grams) Rats body on dry- weight | weight basis Number | Grams Percent SPM: Less than 500_____- 2 434 16. 9 500 to 599___.__._- 3 575 20. 8 G00=to 6992222 === 4 659 23. 0 700 and over____-_- 2 774 29. 7 SPB: Less than 500____-- 3 441 19. 6 500 to 599________- 2 549 20. 1 600 to 699________- Uf 654 19. 0 700 and over_-_-__-_- 1 740 19. 0 SPPB: Less than 500____~- 3 444 17.3 500 to 599_________ 1 550 19. 7 600 to 699_________ 5 667 23. 6 700 and over_____- iG 777 Oso, the same whether the diets contained 10 or 41 percent liver. Reussner and Thiessen (156) reported 29.3 percent fat in the large livers from rats fed an ege and bacon diet, in contrast to 12.5 percent for a cereal and milk diet and 15.1 percent for a milk diet. These differences in liver fat were not related to the level of fat in the diet. In spite of the high liver fat, the bacon and egg diet resulted in good survival of rats on long-term feeding. Okey (140) fed diets containing 1 percent cholesterol to rats from weaning throughout middle and old age, and observed no significant differ- ences in growth, health, and survival between control and cholesterol-fed rats in spite of the high fat and cholesterol content of the livers of the latter. Histological examination of the livers showed fatty infiltration rather than degeneration of functioning tissues. Ridout, Lucas, Patterson, and Best (157) re- ported a progressive increase with increasing dietary cholesterol in the accumulation of both glycerides and cholesterol esters in the livers of rats. Okey and Lyman (143) reported the results of feeding 5, 10, and 15 percent coconut oil or cottonseed oil with or without added cholesterol. The response of female rats was occasionally quite different from males and, for comparison with the results reported in this publication, only the results with males will be discussed. In the absence of dietary cholesterol, elevated liver fats were observed only when coconut or cottonseed oil was fed at the 15-percent level. In the presence of cholesterol, liver lipids and_ total cholesterol increased with increasing concentra- tion of dietary fat and were consistently higher with cottonseed oil than with coconut oil. Okey, Lyman, Harris, and others (145) also compared the response of rats to 13 different fats with iodine numbers varying from 8 to 143, each fed at the 10-percent level. Liver fat and choles- terol values in the absence of dietary cholesterol were not exceedingly high with any of the 13 edible fats investigated. ‘There was a tendency to relatively low values with the more highly satu- rated fats. When cholesterol was included in the diet, liver fat and cholesterol increased; the increase in cholesterol was generally smallest in rats fed fats with low iodinenumbers. According to the author, not one but a number of factors appear to influence the effect of composition of dietary fat on liver lipids. Choline has been found effective in preventing various types of fatty livers in rats (24). In a diet free from cholesterol, Best, Lucas, Patterson, and Ridout (23) reported that regardless of the kind of fat used, total liver lipids were essentially normal when the amount of choline chloride pres- ent was between 0.12 and 0.16 percent. According to Benton, Harper, and Elvehjem (19), the type of dietary fat had little effect on the deposition of liver fat when the diet of the rat contained adequate amounts of choline and protein. Rid- out, Lucas, Patterson, and Best (157) found that the amount of choline needed to maintain liver glycerides within or slightly above normal when the diet contains cholesterol does not necessarily prevent the accumulation of cholesterol esters in the liver. Jackson (100) observed a tendency to fatty livers with degenerative abnormalities when rats were fed a diet containing 80 percent sucrose. This diet, however, did not appear detrimental to growth and general health. On diets containing 45 percent sucrose or starch, no marked differ- ences were observed in size or histological structure of livers or kidneys. Summary.—The results with BHE rats reported in this publication indicate that age may or may not be a factor in the percentage of fat in the liver, depending on the diet under investigation. The increase that occurred with age on some of the diets appeared to be related to an increase in the number of extremely heavy animals on these diets. Differences in liver fat were not neces- sarily related to level of dietary fat. The per- centage of fat in the diet containing beef was somewhat higher than that in the diets containing ege, milk, or peanut butter, but liver fat for rats fed this diet was generally low when compared with the results for the other diets. Liver fats were high in rats fed the diet containing 25 per- cent egg and much higher than were obtained with the extremely high fat diet consisting of 100 per- cent ege yolk. The results with rats fed 8 or 16 percent HVO, lard, or butter also showed no consistent relation between level of dietary fat and liver fat, although these results were compli- cated by the weight loss occurring in moribund rats. Damaged kidneys were a frequent finding in BHE rats regardless of diet, and they were not 15 LG OSE ies: & GS-0 7 Gc‘? ¥ 66-6 “ST 8 “ES oo LOM eS 3s 9 ‘P-8 '€ ov & €6-0 ‘GT 8 ‘8T 06° EV ‘I | 90°E 0 “S-€°G 6:-§ L LAB Si. € GZ 83° ales S076 Pv G-8 '€ 9 ‘PF 8 “€S-0 ‘ST G ‘6 Slew 7 06 °% G ‘P-€ PV GP 6 “81-36 “GT 08ST 610 IG ‘TL | SOE SHG G 9°¢ © tS=0 (26 9 "6% SUDIL) |SUDLY | SWDLD WWIII — | JUIDMAT UII JUIN uloy o8e o5V ysV On ERY | -Olg ODUBYT -1IOAY DDUBYIT -1DAV Ysy YET qUoJUOD [RIOT iL 4yysiom Ap uo poseg v V9-G99 & GL-T ‘09 I €L-8 ‘PE vb PL-€ “8S 8 €L-~% “OL 8 “L9-9 '8¢ TUIDI OSUBY Uld}O1 qu ose ~1IDAY T ‘89 g “G9 IMI | PWIA JOYE M, G GG 6 LT TAT 6ST 8 OT GPL SUDA) VYSIOM OAT onde OAV IT¢ 8GE OSG SGP Lov 866 shog o3v asev -10AW Se 2 ee ooze ddd$ ET | ar ee Doers San dds ON at Se See ene Wds :S7BI poysBjuoN 6 Serene Sie Ae eS ey Adds | An i ea ee Par eee adds [re ean ieee! ira 5 Wds LaQUunny ISYBI poUsByy] SPBYL §yU1 JO JoIp pus UOl}IPUOL) S19 TddS PUY ‘TAS ‘IN dS UO ry vam Burso) syou payspfuou pun payspof wowf saucy ur yso pup Gof 129,04 JT—' EY VILV J, 76 HS 61 'T ¥9 OT * vo. L9G 83" 86 G8 T 8S ° 18 T Li '¥ 02 G8" G9 “ES TS ee Gs ° G8 'E Laue: ZL ‘I (| OF 8 cis 61 T GZS 81° 8ST T 18 € 86° 06 '€ 09 ‘7 1G ° PIT £6 7G GG * Ot 'T 61 GS Ole FILS TZ ° 00 ‘T ZO 'E GG" 56° LE 8 83" va Tg 'S GG © LET 9G "E 66 09% GG PV 83° G6 'T G6 cE ¥8 € 0G P AS IZ 8o 7 Se" TE 7% 60 'T LG" 6G 66'S 0s * IP @ tL Y eS i 10 @ 86 7 8S" 96 T Ge T 83 0 GZ % GV SUDLD | SUDI | SWDLD sy yeq | uloyorg yuoyUod [VIO], v? T GS 6 69 &'P [ervey ¢ ‘OL vv LST 6 89 6S L VG ¢ ‘6S 6 7 TST 0 ‘LL aan ee 0 91 GTZ td 8°16 € Fg 8% € GG § 02 9 °E 9 GS 0 FL 9 '€ 8 '8E g ‘6S co? EWG T 89 & 7 ¥ 1G 0 19 Gv 6 SG G g9 97 6 1G @ ‘99 99 € 61 v 69 Lv 8 GS 6 LO vv 6 FG 0 “9 & 7 8 LG v F9 8°E 0 GZ 9 °¢9 8 ¢ '6E § Sg oP 0 96 G 9G Lv L 6G v 6S 6S Go TE 6 8G 1? 8 66 8 8g vv 6 SS y G9 (a 8 TG G G9 Gv 9 ‘9G G G9 quawad | qwaaiag | jwaaag UsV VET ulojJorIg qystom Alp uo poseg wo © eae Ke) MORO OMlro it Wo) G ‘99 quad JOYR AA 8 LT sor CLL 6IT GOL 8 syed Jest AA L1G 99T LLG i. Wel ee fre se 4904S 0 posuvyo FdS "ZS 60T 989 ‘Pe ie f°) 7S Ad8 0} pasuvyo y90Ig :shVP OCZ 7B pesI9Adzy LOT €éI 968 (6d earn ee Pa na re ae ae Ads GST S6I 06¢ Wi al ce ie late eaaeine areca 99048 —uo penur}u0y 7 9G 0 6G S| operates nn es i aHd$ OLE 0 OGG ns | Cini el anne amine Sinuiin fest haa 9098 ishep oez Apoyeutxoidds ye psoyiwoeg S[BSIOAOL JOT, I FI 9 VCP Ta | Cae oe ee %O'g ‘orngxtur yes+ 16% € LT gE SGP Cy eee er ro See Cr err F OOTA GIG 99 SGP OE mae ig i a ee ads —poj soyeurdoqywy 9 FI 08 GLE 7a (EC Cg oe ee en eee OOTA G LI StI 89¢ :: PEE (deco ea a a: Sg Ge a rena 00TH 8 FI 18 987 GO Seer or een ee tn qoynd 91 d§$ anal L8 €9¢ (PN | he Sas eben Pane art eee 40741nq 8 dS 9 “ST LIT oLG GA a Vege ere ae es pet re plel OT dS ¢ ‘GT T8 87S O18 ieee ag Sat Pee = as cpt wove PIVL8 ds € ‘OT v8 669 Sip 5 ola cg ey ae 0 ate ee OAH 9T d$S 8 0Z est ver Gea «| eee naa as net Ie. Sar %9¥'0 ‘ForaysopoyI + %ZQ ‘plow oIqtoosy Z 61 OST 9EP ae IPT as pe oee ere %SO ‘plow siq1oosy G GS (Gal 607 OP oe Flees Sens %HBE'l ‘Toreysopoup PES OST Ic? Ses. Malte te nao eae %9F'O “Joreysopouy 6 6T 6G1 T&7 OL Ale ee ws QOT/sut go “g + "urs ooT/ su To'O0 “gq +%e'o ‘ouroyp 9 ‘61 ral ol 6 “wi3 QOT/‘sut G0 “G+%G"'O ‘ourfouD € 02 SZI CLD Saati u sae ecu toile ws QOOT/ su ¢'0 “¢ 6 TG FIT PEP a reer | ete i ae, ee eo ee ws ooT/su 100 ““aA+%so ‘ourjoyp 0 1G T&T e9P Sis Bile Save Gare ws QOT/su 100 “Eg GIG 681 G6P Oli le ee ee %e'0 “ourpoupn SsuDiQy | SUDLD shogq | 4aqunn —Y}IM pojyuoulddns qqg s7@1 WHa 4ySIoM SSO] 938 IdAT] YASIOM | oSVIOAY | Sey yop pus urelyg ISVIOAY | OSVIOAY syaup poyuamrsadaa 1ay}0 paf ype WO sua) UL YsD puDd “of “UWI9}0L —FY TTA I, 7] necessarily accompanied by fatty livers. There was some indication with other than SPE diets that large livers tended to be associated with kidney damage and that changes in the liver with age might be important in the production of kidney damage even though the liver itself showed no evidence microscopically of damage. On SPE diet alone or with added supplements, liver fat was high at a relatively early age, often in rats with apparently normal kidneys. The infiltration of the liver with fat that was observed with SPE rats may, however, accelerate kidney changes. The tendency to enlarged, damaged kidneys on all diets suggested an inherent kidney weakness in the BHE strain of rats. It is conceivable that an excessive load may be placed on the kidneys be- cause of improper functioning of the liver resulting from some defect in certain enzyme systems responsible for the normal activity of this organ. Differences in the accumulation of fat in the livers of BHE and Wistar rats when fed SPE diet under identical conditions provide evidence sug- gesting inherent differences in the metabolic proc- esses involved in the utilization of this diet by these two strains of rats. Serum cholesterol Serum cholesterol measurements were usually made on the blood from fasted rats, and only the results with fasted animals are included in this section. RaTs MAINTAINING WEIGHT ON sTocK, SP 8 HVO, anv SPE piets.—The influence of age and diet on cholesterol levels in the sera from rats maintaining weight on stock, SP 8 HVO, and SPE diets is summarized in table 65. Age appeared to exert no influence on the serum cholesterol levels or rats fed stock diet. A cholesterol level of 114 mg./100 ml. was obtained for a 976-day-old rat fed this diet. Cholesterol values were below 150 mg./ 100 ml. in 80 percent of the sera analyzed. On SP 8 HVO diet, serum cholesterol levels were similar to those on stock diet when the age of the rats was less than 500 days. In rats more than 500 days old, however, only 43 percent of the cholesterol values were below 150 mg./100 ml. and 3 of the animals in this group had sera containing more than 200 mg./100 ml. On SPE diet, serum cholesterol levels were high even in the youngest eroup of rats, and were consistently higher than 150 mg./100 ml. for all age groups. Values ex- ceeding 200 mg./100 ml. were a frequent finding, and 9 of these SPE rats had serum levels exceeding 300 mg./100 ml. The high serum cholesterol levels in rats 300 to 399 days of age seem to be another indication that this period is a critical one in the response of rats to SPE diet. TABLE 65.—Influence of diet and age on serum cholesterol levels in rats maintaining weight on stock, SP 8 HAVO, SPE, SPM, SPB, and SPPB diets Serum cholesterol eee EEE EE EE EE ee Diet and age of rats Rats | Average (days) | age Average = as) ae eee ee Number | Days | Mg./100 ml. Stock: Less than 300___-__- 19 229 120 300 to 499_________ 5 410 ila by 500 and over______- 6 691 127 SP 8 HVO: Less than 300_____- 6 252 103 300 to 899________- 8 353 12 400 to 499_________ 6 457 131 500 t0:599- 2 2s2222- 14 546 166 SPE: Less than 300_____-_ 7 252 199 300 to 399__._-__-- 7 353 351 400 to 499_________ 14 455 232 500 16599. ..-22. 11 530 287 SPM: Less than 400_____-_ 6 304 118 400 to 599________- 6 512 164 SPB: Less than 400______ 6 304 103 400 to 599________-_ 6 angst 163 Sees Less than 400_____- 6 289 135 400 to 599________-_ 11 530 221 78 Rats with cholesterol levels of— Range Less than | 150 to 199 | 200 to 299| 300 mg./ 150 mg./ | mg./100 mg./100 100 ml. 100 ml. ml. ml. and over Mg./100 ml. 79-217 79 16 iss 0 97-151 80 20 0 0 107-179 83 17 0 0 90-117 100 0 0 0 81-185 15 25 0 0 112-154 83 U7 0 0 99-270 43 36 21 0) 157-240 0 57 43 0 178-670 0 14 43 43 152-402 0 43 43 14 197-432 0 9 55 36 102-128 100 0 0 0 87-265 50 Uy 33 0 80-152 83 17 0 0 79-243 50 ie 33 0 95-236 83 0) Ur 0 129-343 9 36 36 18 0 ee RATS MAINTAINING WEIGHT ON SPM, SPB, anp SPPB pirts.—The limited data for rats fed diets containing high levels of milk, beef, or peanut butter are reported in table 65. The results for rats fed SPM and SPB diets were similar to those for SP 8 HVO rats. In general, serum cholesterol levels were low in rats under 400 days of age, and tended to be higher in rats 400 to 599 days of age. The slightly higher value for rats under 400 days of age on SPPB diet was due to inclusion of one rat with a value of 236 mg./100 ml., and is of questionable significance considering the limited data available. In the older rats fed this diet, however, cholesterol values were significantly higher (P<0.01) than those for rats of comparable age fed stock, SP 8 HVO, SPM, or SPB diets, with 54 percent exceeding 200 mg./100 ml. RATS LOSING WEIGHT ON sTock, SP 8 HVO, SPE, SPM, SPB, anp SPPB priers.—In table 66 are summarized similar data for rats that were losing weight on these same diets. The results are reported without regard to weight loss before sacrifice. Although there seemed to be a trend toward somewhat higher cholesterol values when weight loss exceeded 100 grams, the data available were too few to warrant separation on this basis. Differences in serum cholesterol with age and with diet were still apparent in the moribund rats. Cholesterol levels exceeding 150 mg./100 ml. were rarely seen in rats less than 400 days old on SP 8 HVO, SPM, and SPB diets. In the intermedi- ate age group, elevated cholesterol values were found with all diets, although the proportion of rats with high serum levels and the extent of the elevation depended upon the dietary regimen of the rats. Except with SPPB diet, rats with a relatively long lifespan tended to have lower cholesterol levels than did those in the intermedi- ate age croup. Rats fed stock or SP 8 HVO diets generally had lower levels than did those fed the other diets. One exceedingly high value of 1,813 mg./100 ml. has been omitted from the average value recorded for stock rats. In this animal the thyroid gland was almost entirely replaced by tumor. On SPE and SPPB diets, the proportion of elevated cholesterol values was high in compari- son with those obtained in rats fed the other diets investigated. CHOLESTEROL AND KIDNEY DAMAGE.—The in- crease in serum cholesterol with age appeared to be associated, in part at least, with an increase in the number of rats with enlarged and damaged kidneys. In table 67 are summarized data relat- ing kidney size to serum cholesterol level. On stock, SP 8 HVO, SPM, or SPB diets, levels exceeding 150 mg./100 ml. were rarely found in healthy or moribund rats with kidneys weighing less than 1.8 grams. Kidneys in this weight range showed little evidence of degenerative change. Somewhat higher serum cholesterol levels tended TABLE 66.—Influence of diet and age on serum cholesterol levels in rats losing weight on stock, SP & HVO, SPE, SPM, SPB, and SPPB diets Diet and age of rats Rats | Average (days) age Average Number | Days | Mg./100 ml. Stock: 500 and over____-_-_- 9 PR 166 SP 8 HVO: Less than 400______ 5 264 91 400 to 699_________ 12 570 182 700 and over______-_ 3 743 113 SPE: Less than 300______ if 189 153 SOOMOVS99 Sots 2 ee | 16 367 386 400 'to15992 222 2 40 497 346 600 to 699________- 12 640 218 SPM: Less than 400______ 4 340 184 AQOtOTD99 22S] 5 501 258 600 and over______-_ 1 600 175 SPB: Less than 400______ 33 268 118 400 to 599-222! 3 535 348 600 and over______- 5 704 162 SPPB: Less than 400______ 12 306 163 AQOtoOjO99s = 22% Set 10 520 276 600 and over______- 2; 663 315 Serum cholesterol Rats with cholesterol levels of — Range Less than | 150 to 199 | 200 to 299] 300 mg./ 150 mg./ | mg./100 mg./100 100 ml. 100 ml. ml. ml. and over Mg./100 ml. 101-235 56 22 22 0 80-103 100 0 0 0 84-385 42 33 8 17 96-129 100 0 0 0 90-248 57 0 43 0 121-904 6 6 19 69 132-932 5 2 35 58 115-290 17 0 83 0 103-419 75 0 0 25 122-573 20 20 40 20 175 0 100 0 0 98-134 100 0 0 0 155-620 0 33 33 33 105-220 40 20 40 0 51-298 67 8 25 0 138-654 30 10 30 30 289, 341 0 0 0 100 TaBLE 67.—Serum cholesterol levels of rats with kidneys of different weights on stock, SP 8 HVO, SPE, SPM, SPB, and SPPB diets Kidneys less than 1.80 grams Diet Rats Rats Average Average weight | cholesterol Number | Grams | Mg./100 ml. | Number Stock 232-2532 = 31 1. 49 108 6 oy egret) s yd 6 Male ee 34 1. 47 114 10 Si es eee SS 27 1. 52 192 10 Oe Mies ee 13 1. 33 115 a oll eee 15 1. 44 113 1 Sila ef: a eee 18 1. 46 147 13 to accompany the small normal kidneys from rats fed SPPB diet. The highest levels to accompany kidneys in this weight range were those from rats fed SPE diet. Kidneys weighing between 1.8 to 2.2 grams frequently showed some signs of damage, chiefly evidenced by the presence of hyalin casts. Cholesterol levels accompanying kidneys in this weight range were consistently higher than those found in rats with small kidneys. Cholesterol values in rats with badly damaged kidneys exceeding 3.0 grams in weight were generally high, but neither kidney size nor the types of kidney damage observed showed any quantitative relationship to the extent that the serum cholesterol was elevated. A further consideration of the cholesterol data suggests that the influence of age on serum levels may be related chiefly to the increase with age in the number of rats with enlarged damaged kidneys. Evidence for this is seen in the data summarized in table 68 for rats fed the semipurified diet, where the results for rats with damaged kidneys have been excluded. The main difference between the results in this table and in table 65 lies in the results for rats 500 days and older. The 5 animals in this age group with small normal kidneys had an average cholesterol value of 120 mg./100 ml. similar to that found for younger rats, in contrast to 166 mg./100 ml. (table 66) for all 14 animals in this age group. TasiE 68.—Serum cholesterol levels of rats with normal kidneys at different ages on SP 8 HVO diet Cholesterol Average age (days) Rats Average Range Number |Mg./100ml.|Mg./100ml. 75 ae ee ae ee 6 103 90-117 5 ie ees ee ee eee 6 119 84-164 7.16) 0 eee eee ene 5 133 112-154 DO tee es oes eee 5 120 99-150 Kidneys 1.80 to 2.19 Kidneys 2.20 grams grams and over Rats pets Average Average Average Average weight | cholesterol weight | cholesterol Grams | Mg./100 ml. | Number | Grams | Mg./100 ml. 1. 94 142 4 2.78 225 1.95 157 10 3. 88 228 1.91 280 76 5. 51 343 1.95 187 3 3. 06 397 1. 86 241 8 3. 32 302 1. 99 222 10 4.45 304 SERUM CHOLESTEROL IN LITTERMATES.—No con- sistent relation of serum cholesterol to liver size, liver fat, and adrenal or thyroid size was observed. There was some indirect evidence that the wide range of serum cholesterol levels was due in part to differences in inherited characteristics in the mixed strain of rats under investigation. Litter- mates were used in each of the experimental series for comparison of the various dietary regimens, but relatively few data were available to compare the response of littermates fed the same experimen- tal diet. One series with SPE diet provided some data that permitted a direct comparison of the response of littermates to this diet under strictly comparable conditions. In table 69 are sum- marized the findings for two littermates from each of four litters. All were sacrificed at about 550 days of age. Data are included not only for serum cholesterol levels but also for the organ weights of these rats. Only one of the animals had lost more than 100 grams when sacrificed. Although the range of serum cholesterol values for the individual rats in the four litters was wide, varying from 152 to 432 mg./100 ml., the serum levels for littermates were relatively close. In contrast, organ weights of individual rats varied considerably even for littermates. Rats FED OTHER EXPERIMENTAL DIETS.—In table 70 are summarized limited data on serum cholesterol levels in rats fed the other experimental diets. Most of the data were for sick or moribund rats that were losing weight at the time of sacrifice. The wide range of values and the tendency to elevated serum cholesterol values observed in SPE rats were also apparent in rats fed the various supplemented SPE diets. No significant differences were observed in the serum cholesterol levels on any of these diets. Most of these rats had lost more than 100 grams before sacrifice and had large damaged kidneys. A comparison of the serum levels in rats that were maintaining weight might show differences not apparent in these moribund animals. When the level of HVO was increased from 8 to 16 percent, the average serum cholesterol level TABLE 69.—Serum cholesterol levels and organ weights for rat littermates fed SPE diet Weight Organ weights Litter No. Identifica-| Age | Maximum at Serum tion No. weight death cholesterol Liver | Kidney | Adrenal} Thyroid Days Grams Grams | Mg./100 ml. | Grams | Grams Mg. Mg. 7 5 513 714 611 267 19. 1 4, 73 46 26 rete gpa ahr Sark alec dRE eRe 2 513 675 657 275 26. 6 2. 58 27 21 8 e 510 530 516 285 16. 9 1. 76 18 15 MGM LM Nar eG 2 511 590 585 220 13.5 2.32 24 20 10 i 504 580 560 432 25. 0 2. 32 18 18 Tae a ee i 2 504 535 500 362 18. 6 1. 88 17 15 13 ‘3 494 563 542 154 16. 4 4, 50 27 19 EU Te aed 2 494 566 511 152 16.8 6. 37 29 27 TasiE 70.—Serum cholesterol levels in rats fed other experimental diets Weight status and diet of rat Losing weight on— SPE supplemented with— @holinepOM pac see ee eee Bro sOOlsme yO ORM 1 te Fe ee Choline, 0.6%+ By, 0.01 mg./100 gm________-____________- Be Oroame lO Oem See etait ee Se coe eos ees Choline, 0.56%+ Bs, 0.5 mg./100 gm______________-_______- Choline, 0.5%+ By, 0.01 mg./100 gm.+ Bs, 0.5 mg./100 gm__ @holesterol0:46.07 sos. os sous fe Ee Cholesterol WSS pe wees se Dike ase fo eae Ee NSCOR DIGHACIO WONZ On sus Nae Sa Ascorbic acid, 0.2%-+ cholesterol, 0.46%___--___- MOG saltiamixiures 3:02 82 oe oe os Se SPW 8 HVO (138 mg./100 ml.) was similar to that observed on the lower level of this fat. With butter or lard there was no evidence that increasing the level of the fats caused any appreciable change in serum cholesterol. Serum cholesterol values, however, tended to be much higher when the dietary fat was lard than when it was HVO. Serum cholesterol values were obtained for rats on only two of the diets investigated to de- termine the influence of egg or egg fractions— SPW 8 HVO and E100. On SPW 8 HVO diet, cholesterol levels were similar to those for com- parable SP 8 HVO rats. On the diet consisting solely of egg yolk, serum values were significantly lower than when the diet contained 25 percent whole egg. Supplementation of egg yolk with salt mixture had little influence on serum cholesterol. Serum cholesterol Rats Average age Average Range Number Days Mg./100 ml. | Mg./100 ml. Ee ene eer 11 483 365 264-700 a Src titen: Li 4 549 296 221-440 3 562 291 204-382 5 512 346 252-510 6 431 334 257-457 u 447 344 196-549 Fern tein ss 8 451 351 261-452 ite TEES 7 422 370 210-470 eps eer ee 5 460 425 224-930 ee eee vi 431 421 164-840 wade a 6 679 138 96-209 Bee a sat 10 548 282 137-674 sa Oe 6 591 285 198-455 ee nas 6 626 204 156-294 rer eae 6 529 168 118-209 Spe eee 10 420 186 115-311 Pattern ee 4 461 213 176-252 Bed yen 2 5 430 190 169-218 clears ies i 6 550 110 80-158 Discussion.—The results reported in this publi- cation as well as those reported by other investi- gators have shown that many factors influence cholesterol levels in the blood, and that the inter- actions between dietary factors and other factors such as heredity, age, and sex make the problem of interpreting serum values a difficult one. Many review articles have appeared dealing with various aspects of cholesterol metabolism. Kritchevsky’s (109) book provides information on the biological significance and function of cholesterol. Portman and Stare (153) reviewed the many factors im- portant in the dietary regulation of serum choles- terol levels. Deuel (46) covered many phases of lipid metabolism in relation to blood cholesterol levels. Discussion of the literature dealing with serum cholesterol will be limited to those investigations 81 that seem most closely related to the results reported in this bulletin. Blood cholesterol levels vary considerably among species and only reports dealing with rat as the experimental animal will be considered, along with a few of the reports dealing with humans because of interest in the possible application to humans of the results obtained with rats. The results for rats will be confined to those reported for male rats. The response of serum cholesterol levels to diet often differs, depending upon the absence or presence of cholesterol in the diet. Comparison of serum cholesterol values, therefore, must take into account whether we are dealing with en- dogenous cholesterol or with serum cholesterol levels that may be reflecting both endogenous and exogenous cholesterol. The liver is the chief source of endogenous cho- lesterol and not only is able to synthesize this sterol but also is active in its breakdown and excretion. Serum cholesterol values in the ab- sence of dietary cholesterol generally reflect the balance of activity in the liver with regard to these two processes. With humans, serum cholesterol levels reflect lifetime dietary habits which generally include the consumption of cholesterol-containing foods. With the rat, however, many investigations deal with diets that contain little or no cholesterol so that blood cholesterol is strictly endogenous in origin. Even in the absence of dietary cholesterol, the results relating the kind and/or level of fat to serum cholesterol levels of rats are not entirely consistent. In the absence of dietary cholesterol, several in- vestigators report a tendency for blood cholesterol levels in rats to increase with increasing unsatura- tion of dietary fat. Klein (105), feeding diets containing 5- and 30-percent levels of Crisco or corn oil found plasma cholesterol levels to increase as the intake of linoleic acid increased. Swell, Flick, Field, and Treadwell (179) reported in- creased levels with increasing unsaturation of fat when rats were fed diets containing soybean fat hydrogenated to different iodine values. Nath, Wiener, Harper, and Elvehjem (136) reported little effect on serum cholesterol levels in rats as the result of feeding increasing quantities of hy- drogenated coconut oil but a slight increase when 1 percent corn oil was added to the diet. Sun- flower seed oil (79) has also been reported to elevate blood cholesterol. With diets in which sucrose was the carbo- hydrate, Marshall, Hildebrand, Dupont, and Womack (126) obtained significantly higher cho- lesterol levels with 15 percent corn oil than with 3 percent corn oil or with 15 percent lard or HVO. In contrast, no significant differences in serum levels were observed when the carbohydrate was starch. Okey, Lyman, Harris, and others (144) reported that the degree of saturation of dietary fat exerted little influence on serum cholesterol levels when 10 percent of fat was added to a nutri- 82 tionally adequate synthetic diet. Best, Lucas Patterson, and Ridout (23) also reported that the kind of fat had little effect on serum cholesterol when the diet contained sufficient choline to pre- vent fatty livers. Aftergood, Deuel, and Alfin-Slater (6) found no significant difference in the plasma cholesterol levels of rats fed a diet containing 15 percent cottonseed oil or lard after a 12-week feeding period. After 24 weeks, however, plasma choles- terol levels were significantly lower in rats fed cottonseed oil than in those receiving lard. Avigan and Steinberg (15) reported an increase in serum cholesterol when either coconut oil or corn oil was added to a Purina chow diet, but the increase was ean greater with coconut oil than with corn oil. There is at present no satisfactory explanation for these divergent findings. The results for BHE rats reported in this publication indicate that, in the absence of dietary cholesterol, serum levels change slowly with diet, and suggest that some of the discrepancies in the literature may be due to the relatively short feeding periods generally studied. Another factor that may be responsible for some of the differences observed is the hered- ity of the strain of rats under investigation. Kohn (106) reported evidence for considerable variation among strains of rats in their average serum cholesterol values which varied from 65 to 132 mg./100 ml. Investigations of the influence of dietary cho- lesterol on the serum cholesterol levels in the rat have dealt chiefly with the addition of cholesterol per se rather than with the use of cholesterol- containing foods. The response to feeding these cholesterol-containing diets is apparently in- fluenced by accompanying dietary components. Dietary cholesterol may be absorbed by the rat in the absence of dietary fat, but the presence of fat in the diet results in an appreciable increase in serum cholesterol levels (33). The fatty acid component, not glycerol, is reported to be the important factor (179, 189). Dietary cholesterol may result in elevated values in the blood in the absence of fat if sufficient bile salts are fed (179). Wilgram, Lewis, and Best (189) reported increased cholesterol levels when choline was added to a diet containing cholesterol. Unsaturated fats tended to lower serum cho- lesterol levels of rats fed cholesterol in contrast to the elevated values reported by several investi- gators when this sterol was absent from the diet. Okey and Stone (147) and Aftergood, Deuel, and Alfin-Slater (6) reported lower serum values with cottonseed oil than with lard, and small but com- parable differences in liver lipids. The latter investigators report that the differences in serum levels observed were not due to differences in the absorption of these two fats. The addition of large amounts of vitamin E to the lard diet eli- minated the differences observed in the liver lipid but did not influence blood cholesterol levels. Nath, Wiener, Harper, and Elvehjem (136) demon- strated a marked accumulation of cholesterol in the blood and livers of rats fed 1 percent cholesterol and 10 percent hydrogenated coconut oil, although no accumulation was observed with cholesterol or coconut oil when fed alone. Replacement of 1 percent of the coconut oil with an equivalent amount of corn oil resulted in a marked decrease in blood and liver cholesterol and in a proportion- ately greater decrease in total liver lipids. Shapiro and Freedman (1/70) found that the addition of safflower oil and methionine to a cholesterol- containing and sulfur-deficient diet was more effective in reducing hypercholesterolemia than a supplement of methionine with a hydrogenated fat (Crisco). No exceedingly high levels of cholesterol were observed with the 13 fats in- vestigated by Okey, Lyman, Harris, and others (145) even where cholesterol was included in the diet. The highest value for male rats was 96 mg./100 ml. when coconut oil was fed. Lower values were associated with the more highly un- saturated fats, but there was no consistent trend relating serum levels to the degree of saturation of the dietary fat. The influence of the unsaturated fats on serum cholesterol levels does not appear to be related to absorption of the sterol. Lin, Karvinen, and Ivy (117) and Ivy, Lin, and Karvinen (99) reported a limited capacity for cholesterol absorption based on measurements of fecal excretion. Byers and Friedman (37) compared the immediate response of rats to cholesterol added to the diet in soybean oil, corn oil, lard, or coconut oil as determined by measurements in intestinal lymph, and found absorption to be greater with the unsaturated than with the saturated fats. Okey and Lyman (143) observed a difference in response to dietary cholesterol depending on the level as well as the kind of dietary fat. Choles- terol levels tended to be higher when cholesterol was fed with 10 percent coconut oil than when fed with an equivalent amount of cottonseed oil. At the 5- and 15-percent levels of these two fats, however, no significant differences were observed. Very little has been reported on the serum lipids of rats fed cholesterol-containing foods. Blather- wick, Medlar, Bradshaw, and others (31) reported high plasma cholesterol as well as fatty livers of high cholesterol content as the result of feeding diets containing large amounts of beef liver. Reussner and Thiessen (156) did not determine blood cholesterol values for rats on the cereal and milk or egg and bacon diets, but did obtain evidence of differences in the serum lipid components based on flotation rate measurments that showed a much higher value for the S; 12-400 class for the bacon and egg diet than for the cereal and milk diet. Rosenkrantz and Bruger (162) found that the feeding of egg yolk resulted in an elevation of the cholesterol content in blood and liver. Although there has been some evidence that dietary cholesterol has little influence on the serum cholesterol of human _ subjects, evidence has increased indicating that under some circum- stances dietary cholesterol may be an important factor in determining serum cholesterol levels. A single dose of 10 grams of cholesterol fed in a meal with ample fat caused only a small and transient change in the serum cholesterol of young men (1/04). Serum cholesterol levels may be elevated, however, as the result of consuming cholesterol-containing foods such as ege (34) or butter (25, 26). From investigations of the re- sponse to various fractions from butter with and without various supplements, Beveridge, Connell, Haust, and Mayer (25) showed that relatively small amounts of cholesterol, depending on the dietary fat with which it is associated, may effect highly significant increases in plasma cholesterol in man. Beveridge, Connell, Mayer, and Haust (27) fed varying levels of cholesterol with a homogenized diet containing 30 percent of the calories as a butter-fat fraction low in cholesterol to a group of university students for a period of 16 days. Between intakes of 13 and 634 mg. of cholesterol daily, serum cholesterol levels increased sharply, but no further significant increases were obtained with daily intakes of 1,300 to 4,500 mg. Cook, Edwards, and Riddell (43) reported 15 percent absorption of crystalline cholesterol by one subject (male) in contrast to 60 percent when ege was the source of the sterol. Y9049 aSBIDAY | S}BY (sfep) Syel Jo ode pus JIC sabn quasaffrp yo yybran Burso) syou wouf dias fo spuawod wos Ur9},01q— EL ATAV J, 88 TaBLE 73.—PA,!' serum cholesterol, kidney weight, and kind and extent of kidney damage in rats fed SP 8 HVO and SPE diets Serum cholesterol ? Kidney weight Rating of kidney damage Diet and PA Rats | Average} Average range age PA? Average| Range | Average} Range Hyalin | Cystic | Glomer- ular Mqg./100| Mg./100 SP 8 HVO: Number | Days | Percent mi. ml, Grams Grams Score Score Score PA, none_______- 25 426 117 81-185 Wal .2- 3.4 0. 3 0 0 Less than 4.0___- 10 493 PT 129 112-192 1.5] 1.2- 2.0 .3 0 0 AnORtor (es == 2 6 520 6.8 188 108-278 2.5 | 1.8- 4.5 1.5 0. 5 0.5 a SO and over-_-__-- 1 544 17. 6 270 270 5. 2 5. 2 2.0 3. 0 2.0 SPE: PAS Nome. =.= 2- = 20 405 0 225 152-326 $2.2 | 1.5- 4.7 1.2 4 se Less than 4.0__-- 8 419 2. 6 286 132-434 2.1 | 1. 8- 3.6 Lee, 0 0 AO. COnG9 a6 22 - 15 426 Beak 314 152-437 4.8 | 1. 6-10. 1 1.8 1.5 .9 8.0 and over___-- 12 434 11.7 488 237-743 6.1 | 1. 8-11. 2 2.1 2. 7 1.2 1PA represents prealbumin component or components moving more rapidly than albumin. 2 PA values represent relative percentage of serum protein components. per 100 milliliters in serum. Cholesterol values represent milligrams 3 Omitting one rat with kidney weight of 13.2 grams—no PA and cholesterol 202 mg./100 ml. PA was absent from the sera, cholesterol levels were generally low and the kidney small with little evidence of damage. Only 3 of the 25 rats in this group had kidneys exceeding 2.0 grams in weight, and only 5 had serum cholesterol levels exceeding 150 mg./100 ml. When the PA level in the serum was less than 4 percent of the serum proteins, cholesterol levels and kidney weights were similar to those for rats with no PA in their sera. When the PA level was above 4.0 percent, kidneys were generally enlarged and cholesterol levels exceeded 150 mg./100 ml. in all but 1 rat. On SPE diet a similar trend was observed, although the range of values was wide for each group. In the group of 20 rats with no PA in their serum protein, only 1 had a serum cholesterol level in excess of 300 mg./100 ml. and 2 of the kidneys exceeded 3.0 grams in weight. Excluded from the data was 1 rat with a very large kidney weighing 13.2 grams. This rat had no PA in the serum and a serum cholesterol of 202 mg./100 ml. In the group of 12 rats with PA levels 8 percent and over, only 1 had a serum cholesterol value of less than 300 mg./100 ml.; the others had values exceeding 400 mg./100 ml. Three of the kidneys weighed less than 3 grams; eight exceeded 6.0 grams. In general, high concentrations of PA in serum proteins tended to parallel serum cholesterol levels somewhat more closely than kidney size. Rats rep SPM, SPB, anp SPPB pirts.—In table 74 are summarized limited data from electrophoresis of the sera from rats fed SPM, SPB, and SPPB diets. There were insufficient data to establish the influence of age. With each of these diets the fast-moving component was present in the sera of some of the rats, even among the relatively young animals. The highest level observed was for a rat fed SPM diet, with the fast-moving component representing 20 percent of the serum proteins. The other serum proteins were present in amounts that were similar to those found in rats fed SP 8 HVO or stock diets. Discusston.—Many factors have been shown to influence the results of electrophoretic studies of blood proteins, and hence to complicate com- parisons of the results of such studies (28, 49, 133). Concentration and kind of buffer, optical devices used to resolve the protein concentration gradients, and species, strain, sex, and age of the experi- mental animals may all be determining factors. Many of the investigations have dealt with at- tempts to characterize certain pathological condi- tions by means of the electrophoretic pattern of the blood proteins. There appear to be no reports of investigations comparable to those included in this bulletin dealing with the electro- phoretic pattern of the blood proteins of rats on various dietary regimens throughout their lifespan. In 1945, Deutsch and Goodloe (49) investigated the plasma proteins of 20 species of animals and obtained evidence of a small amount of protein migrating more rapidly than albumin in certain species, including the rat. These authors reported poor electrophoretic separation for some of the proteins in the blood plasma of rats. Halliday and Kekwick (83) reported, in the blood of young rats, a component moving ahead of albumin, possibly a second albumin, which varied in con- centration from 8.6 percent at 12 days of age to 4.2 percent at 90 days. Total albumin increased from 60 to 70 percent of the total protein during this period.