An Investigation of Operant Elements in Desensitization: A Connparison of Differential Reinforcement of Other Behaviors and Desensitization in the Reduction of Phobic Responses in Rats By ARTHUR M. WELLS, JR. A DISSERTATION PRESENTED TO THE GRADUATE COUNCIL OF THE UNIVERSITY OF FLORIDA IN PARTIAL FULFILLMENT OF THE REQUIREMENTS FOR THE DEGREE OF DOCTOR OF PHILOSOPHY UNIVERSITY OF FLORIDA 1970 To My Wife ACKNOV/LSDGSHEi^TS The author wishes to acknov/ledge his indehtedness and to ex- press his ;:^ratitude to those v.'ho gave so willingly of their guidance, support, and patience. First of all appreciation is due to the Supervisory Coamittee Chairi'ian, Dr. Harry A. Grater, Jr., and to all mem'bers of the Supervisory Cocunittee, Dr. Benjamin Barger, Dr. Hugh C. Davis, Jr., Dr. Mary lou McSver, and Dr. Henry S. Pennypacl:er. Appreciation is also due Dr. Frederick A. Xing for his kind assistance with experimental equipien.t and to Dr. Edv/ard F. Malagodi, Jr., for his lean of equipment and lahoratory facilities. Thanks are due also to Mrs. Irma Smith for her careful preparation of the m.aiiuscript. Finally, special gratitude goes to the author's wife-., Anastasia, to v;hora this study is dedicated. She provider an invaluable source of meaning to this work and to the achieveaent it reoresents. ill TABLE OF COHTEI^TS Page ACKNOWLSDaMSOTS iii LIST 0? TABLES v LIST OF FIGaRiS vi ABSTHACT vii INTRODUCTIOIJ 1 METHOD 13 RESLTl.TS 25 DISCUSSION /}1 REFEIlSiCESo 51 APPEl^iDlCL^ 54 APPENDIX A S5 APPENDIX B 56 /J'PENDIX C 57 BIOGRAPHICAL SKETT-H 58 iv LIST OF TA5LES Ta.-hle P^2 1. ONSET AKD DURATION 0? TONE MI) IX)OD PRiSSNTATION IN TRSATMEt^T 19 2. ANALYSIS 07 7ARIANCE 0? AVOIDANCE RESPONSE OF THRE3 GROUPS ON THE EIETH DAY 0? ACQUISITION TRAINING 2? 3. DAILY TOTAJ.. NUMBER OF PHOBIC RSPONSSS MADE BY THE OPERANT ANII-'IALS OYER FIVE TREATMENT DAYS 29 4. MEA-MS ^m) STAl'IDARD DEVIATIONS OF >IU]1BSR OF PHOBIC RESPONSES MADE DURING THE FIVE DAYS OF TEN TRIALS PER DAY EXTINCTION PHASE 32 5. ANAJ.YSIS OF VARIANCE OF NUl-IBER OF PHOBIC RESPONSES I'ulDE Bf THE THREE GROUPS OVER FIVE DAYS OF EXTINCTION .... 33 6. POST-HOC COilPARISONS OF THE THREE GROUPS IN ilFMBFJl OF AVOIDANCE R5SP0N3JS DURING EXTINCTION. 3-'^ 7. MEANS AND ST/JIDARD DEVIATIOIS OF CS - OR LATE^TCIIS OF THREE GROUPS OVER FIVE EXTINCTION DAYS 38 8. ANALYSIS OF VAPJ/JCE OF CS - CR LATENCIES OF THREE GROUTS OVER FIVE EXTINCTION DAYS 39 9. POST-HOC COMPARISONS OF THE THREE GROUPS IN LAI'ENCY OF AVOIDANCE RESPONSE DURING MTINCTION 40 LIST OF FIGURiS rigor e Page 1. Mean number of aToidance responses of three groups of nine subjects over five days of conditioning 26 2. Mean nujnber of avoidance responses of tl-n^ee groups of nine subjects over five extinction days 31 3. Average time betv;een CS onset and phobic response of three groups of nine subjects over five extinction days 37 Yi Aostracl of Dissertation Presented to the Graduate Council in Partial i\ilfillcent of the Require:: entr, Tor the Degree of Doctor of Philosophy at the University of Florida M INYESTIGATION OF OPERAITT ELE1-3NTS IN DESffilSITIZATIO}!: A COMPARISON OF DIFFiiL^.aiTIAL RSniFORCSISiT OF OTHER B3HAVI0RS A?iD DSSENSITIZATION IN THE REDUCTION OF PHOBIC PJISPOIISES IN MTS Arthur K. Wells, Jr. March, 19?0 ChairEian: Dr. Harr/ A. Grater, Jr. Major Dspartnent: Psycholo^ An animal analogue studi' was designed to conpave the effectG of desensitization and operar:t conditioning In reducing phobic tehayiors. The intent of this comparison was to investigate opDiV-nt ilcirents in desensitization rather than to evaluate the relative sfficacv of the two treatments. On the tasis of previous research an e:yplana.tion of desensiti- zation in terms of operant rather than classical conditioning principles was thought to be feasible, leading to the follov;ing hiypotheses: 1. An operant treatment procedure similar to an operant pro- cedure possible in desensitization and presented within the framework of desensitization facilitates the extinction of phobic behavior:" more so than a desensitization treatment in which operant elements exe cinimizedj thereby demonstrating the dependence of desensitization on operant con- ditioning. ?.. Both the operant and desensitization procedures facilitate the extinction of phobic beliaviors to a greater extent than does a Tii control procedure, therety demonstrating treatment effects. To test these hypotheses, rats v'ere conditioned to hurdle juip in a shuttle hox to avoid electric shock \:hich followed a 500 llz. tone (C3). After five days of avoidance conditioning the rats v;ere assigned to tv;o treataent and one control groups, matched according to their strength of avoidance responding. Shock then v;as discontinued and treatment began. Both treat- ment groups received eq.ual exposure to the CS tones (phobic stimuli) which were presented in hierarchial arrangement. Initially the treat- ment CS was an 8,000 Hz., 66 db tone presented for two seconds, follo-.;- ing t'jo seconds of a seven second food presentation. On subsequent treatment days the CS becane lower, louder and longer. By the fifth (final) treatment day it was equal to th3 original CS tone used in avoidance conditioning. Food presentation increasingly followed CS onset in the operant procedure and was made contingent upon the animal's fflaking various inhibitory responses involved in confronting the CS for increasing periods without making an avoidance (phobic) response. Koweirer, in desensitization the tone onset followed and was concomitant with food presentation. Following treatment all three groups received five dsjs of extinction, during which the number and latencies of the phobic responses •rfere recorded. Statistical analyses revealed the following: 1. The hj-pothesis of superiority of the operant procedure as compared with dCiGnsitisation in reducing phobic behaviors v;as rejected. viii In fact, the deEie.nsitization procedure demonstrated a stronger treat- ment effect—though not significantly so. 2. Both treatments resulted in less resistance to the extinction of photic behaviors than did the control procedure. The results of this work are consistent with the view that the treatment of phohic ^jehavior may "be accomplished either hy rewarding phohic object approach behaviors at the motor level or by the desensiti- zation of anxiety at a more internal level. These results further support a unified view of behavior and experience which v;ould regai'd changes occurring at one level of overt or covert beliavior as having implications for cha.ngc:s at other such levels. A futui'e determination of the most effective of these two treatments could be accomplished within the experimental framework here introduced by removing certaj,n restrictions which were placed on the operant procedure. These restrictions were regarded as necessary in this present study in order to ma2:e the operant treatment more comparable to the operant procedure involved In desensitization. Desensitisation with humans could perhaps be made more effective by employing and maximizing conditioned positive reinforcers, making rewards contingent upon the subject's report of successful progression thi'ough the hisrarcliy of threatening stLtiulus situations. ix INTRODUCTION Desensitizt.tion techniques as introduced '07 V/olpe (I958) have been successfully employed ty many therapists for the relief of pri- marily psychoneurotic symptoms (V/olpe, 195S; Lazarus, 19^1; Lang and Lazovik, I963; lazai-us, I963, and Paul, I966). This study represents an attci'ipt to further our understanding of the processes by v;hich dessnsitization success is achieved in reducing phobic behaviors. Fears and phobic behaviors are comrion distressful problems and the treatment of these problems is worthy of investigation. The term £hoMc behavior is used here in agreement with tfolpe's (1958) definition as behavior wliich is persistently mal- adaptive in view of changed environruGutal conditions of reinforc^ricnt. According to classical conditioning theory (Pavlov, I927) such phobic behavior is esta'^lished when a previously neatral stimulus situation is followed closely by punishment. The neutral stimulus v;ill be referred to as the conditioned stimulus (C3) while the punishing situ- ation will be regarded as an unconditioned stimulus (UCS). After re- peated presentations of the C3, follo.-;ed closely by the UCS, the subject (S_) reacts to the CS in a manner similar to his reaction to the UCS. This overt avoidance behavior, termed the conditioned response (CR), is generally accepted as a measure of fear on the part of the S_ learning this phobic behavior. The persistence of phobic behavior in response to the CS, lorg after removal of the UCS, wry be regarded as unadaptive behavior. Treatment of phobic behavior will be defined here as any process which facilitates, or otherwise results in, the reduction of the overt phobic behavior as a response (CR) to the CS when the UCS ceases to occur. In this study shock (UCS) will he used to con- dition auinals to jump (CR) over a hurdle frcni one end of a shuttle box to the other in response to a tone (CS). This jumping behavior following onset of the tone persists long after the shock is discon- tinued. The jumping is regarded as a phobic response rather than an avoidance response under these conditions. A pai^ticular interest here is with the explanation of treatment of phobic responses by desensiti- zatlon. £oijjit_eix:Oj;iditiqnijj|^oi;_^es_en^^ Couiiterconditioning is the tera sonetiaes used in describing experimental laboratory v.-ork with animals which is analogous to desensitization in clinical treatment v/ith ha-nans. However, both teri.!S describe essentially equivalent p'l'ocesses (Uolpe, 1952; i?5'3). Wclps bases his desensitization technique on early animal studies using shock as the UCS and using eating responses on the pfvrt of the S_ as the reciprocal inhibitor to the previously conditioned fear (Wolpe, 1952 j 1958). This approach was similar to ths.t employed by VJatson eM Rayner (1920) and Jones (1924); a previously conditioned phobia in a child was treated with food given to the child at progressively diminishing distances to the phobic object. In a similar vein, flooding (Weinberger, I965) is the laboratory animal study equivalent of implcsive therapy in treating humans (Sta-apfl and Levis, I967). This latter treatment involves repeated and continued presentation of the CS until the CR diminishes, in a massed extinction procedure. The treatment procedure of desensitizatiorij as describsd by V.'olpe (1958), will be employed here with anicals. This treatnant in- voItos having the phoMc aniaal experience the phobic stimulus while eating food. The CS or phobic stimulus is presented along a dimension of stimulus generalization ranging from weakest to strongest in terms of CR evocative potential. V/hile the animal is eating, the C3 is presented in increasingly stronger forms until the original CS form Is presented. In working with humans the first step in desensitization is constructicn of a hierarchial arrangement of stimulus situations which evoke the phobic behavior, arranged from weakest to strongest in terms of their evocative potential. After rali-Tation training, the subject is asked to imr^gine these stimuras situations while remaining relaxed. Wolpe caution^, that the new responce to be learned (relaxation) must be stronger than the unadaptive response to be eliminated (anxiety). Thus, by presenting weak portions of the C3 first, and by not progressing to a stronger CS item until all anxiety in response to that CS item has been reciprocally inhibited by the reciprocal inhibitor (relaxation), desensitization will be maintained without hindrance of sensitization (conditioning instead of removing anxiety in response to the CS). This caution on Wolpe's part is important, though not necessarily for the reasons which V/olpe would offer. Gale, Sturmfels, and Gale {I966) found that though this use of progressive approximations in presenting these threatening stimuli (C3) facilitates extinction, this method is most effective v;hen used in conjunction with desensitization. Goldstein's (1967) carefully controlled study suggests th;-.t desensitization is more effective than extinction alone onlx v.'lien progressive approxiraation in presentirxg the CS is employed; without hierarchially arranged presentation of the CS, there is no significant difference between extinction and counterconditioning. Thus, the procedure of progressive approxiraations in presenting the CS is of therapeutic importance, particularly in conjunction with the desensitization treatment method. 9l£l^ ical Conditioning- Explanation of Desen'sitisatioii I'/olpe (1958) offers an explanation of desensitization based primarily on classical conditioning principles. Wolpe's explanation of desensitization treatment of phobic behavior primarily involves the "masking" or reciprocal inhibition of anxiety by a reciprocal in- hibitor, i.e., a response state which is antagonistic to anxiety and which prevents the occurrence of the anxiety. In desensitization, cues which originally evclsed anxiety and phobic behavior come to be associ- ated v;ith and come to evoke relaxation, which being antagonistic to anxiety comes to eliminate the anxiety aid phobic behavior. Wolpe (1961), using his principles cf reciprocal inhibition, clearly suggests that in desensitization it is the inhibiting properties of the incompatible responses (relaxation) that reduces araiety and thus eliminates phobic behavior: "if a response inhibitory to anjciety can be made to occur in the presence of anxiety-evoking stimuli so that it is accompanied by a complete or partial suppression of the anxiety response, the bond between these stimuli and the anxiety response will be weal:ened" (VJolpe, I96I, p. I89). Note that '..'olpe believeo that ;.'hat is treated is the anxiety and not its outv:ard raani- festations in the foriu of oLserrable phobic behavior. Also note th?.t Wolpe believes it is the associations between the C3 and internal response states, such as anxiety and relaxation, which are changed in desensitization treatment. As previously noted, counterconditioning of laboratory animals is the equivalent of the desensitization process. In his research with animals, VJolps (1952) used eating responses as a reciprocal in- hibitor and argued that the neural and physiological responses associ- ated with eating and those associated with fear cannot occur simul- taneously because the first are antagonistic to the latter. Thus, in desen3iti7:ationj eating responses reciprocally inhibit fear and thereby decrease and eliminate those beMviors based en such fear. This study investigates desensitization with aninals cmployiiig eating responses as the reciprocal inhibitor. Cautela {I966) offers an explanation of the desensitization process in ter^ns of Pavlovian theory. V/hile relaxed, the subject's cortex is thought to be in a predominantly inhibitory state when the excitatory stinulus (CS) is presented. The excitatory stimulus thus loses its anxiety arousing potential after desensitization. This ex- planation is similar to V/olpe*s in that cue-anxiety bonds are weakened as a result of strengthening of cue-relaxation bonds. Reciprocal Inhibitors as^Rej-vards Relaxation responses are nost commonly used in desensitization as a reciprocal inhibitor of anxiety. EovevGr, a wide variety of other such inhibitors have heen eiTiployed including assertive responses (Wolpc &M Lazarus, 1966), dinigs which induce relaxation (IJolpe and La?anis, I966) , a comforting relationship (Bentler, I962), emotive or enjoyable iroasery (Lazarus and Ahranjovitz, I962), playing responses with children (White, 1959), and eating responses ('/Jolpe, 1952; 1958). An elcnent coiar.on to all these reciprocal inhihitors is their potential reinforcement value in operant conditioning. Solyon and Miller (196?) h-ave used withdrav/al of punishment (termination of shock) instead of relaxation as a "reciprocal inluhitor" in treating pLohias. The fact th^t reciprocal inhihitors can also he positive reinforcers suggest? the possilility of operant processes in desensitization treatment of phohic tehavior. Operant Processes in Desensitization Desencitization involves having the su.hjcct imagine increasingly threatening scenes vhile he is in a state of relaxation. The seq.uence occurs as follows: relaxation—therapist's su^estion of the imagination of an anxiety provoking image (the C3)-»suhject's report of having successfully iaiagined the scene without disturbance — therapist's suggestion that the subject relax (Wolpe and Lazarus, I966, p. 81). The subject reports v/hether or not he is disturbed over imagining the scene by signaling with raising his finger. B:,'- slowly progressing through the hierarchial list of threatening scenes, from least to most threaten~ ing, anxiety experiences on the part of the subject can be eliminated or kept to minisial levels. Although 7/olpo offers an explar^ation of this process in terms of classical conditioning principles as previously described, an alternate erplanation in terms of operant learning principles appears to be tenable and more parsimonious. Relaxation instructions or any other "reciprocal inhibitor" would serve as a conditioned reinforcer in sucli an operant explanation. The behavior rev/arded would be the subject's self-report of having imagined a threatening scene. As previously noted this self-report is given via finger signal in the desensiti nation procedure. As the subject reports that he has suc- cessfully imagined threatening scenes without experiencing disturbance he is then operantly conditioned to progressively continue in this process until he has imagined the most anxiety provoking scene in the hierarchy. If the subject reports being disturbed in the descnsitization procedure then he is presented with previously given discriminative stimuli in the hierarchy and must progress back through the hierarchy again. This procedure could be regarded as operant conditlouir^ of finger elevation (representing the person's report of successful imagination of the threatening discriminative stimulus) in the presence of a fading procedure v;herein the discriminative stimuli progress in presentation along a stimulus dimension from least to most threatening in terms of phobic response evocative potential. The alternate explanation of desensitizatiou in operant terms involves modifying a response by bringing behavior under stimulus control rather than by hypothesizing the reduction or elimination of anxiety, upon which the phobic behavior is based. The sequence of events in descnsitization plus the reward potential of reciprocal inhibitors make such an alternate exolanation feasible if it can be 8 shovm that reduction of anxiety is not a necessary element in eliminating phoMc tehavior. VJolpe, as previously descri'oed, attributes treatment success of photic tshavior as due to reduction or elimination of anxiety. The successful modification of phohic "behavior using an operant procedure vould show that reduction of amiiety is not a necessary eleir.ent in the reduction of phohic behavior, though it might still be regarded as a sufficient element. Studies SuriT-'Ortive of an Operant Exjjlanation" Studies which have attempted to analyze and study the components of desensitization 1-iave shovm that whereas relaxation has no treatment value alone (Cooke, I968; Johnson and Sechrest, 19^8), px-essntatio'i of the C3 in hierarchial form without usiri^^ relaxation does Lave treatment value (Cooke, I968; Ra,chman, I968; and Rachman and Hodgson, 19^7) • Thus, it appears that relaxation to counteract ajixiety is not a necessary treatment condition; ar^iety does not need to be directly eliminated in the successful treatment of phobic behavior. Wolpin and Raines (I966) found that having subjects simply imagine the original full CS (most threatening scene in the hierarchy) for more than two minutes resulted in extinction of the phobic behavior. Implosive therapy, which involves the technique of flooding whereby the subject is repeatedly presented with the CS in massed and prolonged extinction trials, has been successfully used in eliminating phobic behavior (Hogan and Kirciner, 1963; Stampfl and Levis, I967). These procedures also mal:e no attempt to minimize or reciprocally inhibit anxiety. V/olpc biio. Lazarus (I966, p. 1^-0) believe that v;hen such treatment is sonetimes successful , it is only teca.usc of "transsiarginal (protectivG) inhibition . . . the diminution of response that is ol>- servecl vhen stimulus intensity exceeds a certain limit." Hov/ever, this does not explain the lasting effect of such treatment to all degrees of stimulus intensity in CS confrontation. Reduction of phobic 'behavior is facilitated or phobic behavior is successfully treated v/hen the subject is prevented from raalcing the unadaptive response v;ith repeated presentations of the CS without any presentations of the UCS (Carlson and Black, 1959 j Weinberger, I965). In these studies the subject was prevented from makir^g the unadaptive response (phobic behavior) ly utilizing a barrier in a shuttle box, where jumping from one end of the box to the other upon presentation of the CS V7as defined as the phobic behavior. An operant method of reinforcing the subject to stay in the situation vhen the CS is presented without the UCS should be equally successful in facilitating elimina- tion of such phobic behavior, demonstrating the possibility of operant processes in desensitization. Lang and Lazovik (19^3) reported that following desensitization of snake phobia, using relajcation as a reciprocal inhibitor, seme sub- jects were able to demonstrate treatment success by touching a snake, in spite of the fact that after treatment they reported no decrease in their pre-treatment anxiety concerning touching a snake. This is exactly what vrould be expected in terms of an operant learning view of desensitization technique. Other subjects who reported a decrease in anxiety levels following treatment may have been responding to the demand characteristics of the experimental situation (Orne, I96Z) or 10 the e>:pectations of th3 erpsrinenter (Rosenthal, I966), Another possihility for dscrenent in anxiety is that airciety vfas experienced and then extingaished in the absence of the UC3 during desenpitization treatment. Results of studies cited in this section all demonstrate that anxiety does not hs.re to he reciprocally inhibited in the treatment of phohic hehavior. This fact coupled with an alternate explanation cf events in the desensitization procedure in terms of operant conditioning leads to the hypothesis that at least part of desensitization success is due to operajit procedures. Formulation of. ,.the^ Hypotheseg Treatment of phohic heha?ior may involve facilitation of ex- tinction processes. These extinction processes do not al\:o.ys operate. ^Ihevi the subject cakes an unadaptive response (CR) in response to the CS, this cay preclude the subject from experiencing the absence of the UCS. Fii.i.'theraore, the frequently short latency betv.'een onset of the CS and the occurrence of the CR may serve to prevent an anxiety reaction, thus conserving conditioned anxiety from undergoing extinction (Delude and Carlson, 196k ; Solonon and Wynne, 195^). Desensitization, operant techniques, flooding/carrier methods, and other procedures used in treating phobic behavior are considered here to be successful to the extent th^t they allow, encourage or force the subject to experience both the C3 and the lack of the occurrence of the UCS, so that extinction caji occur. 11 Wolpe's eiqilanation of descnsitization in terms of a primarily classical conditioning paradigm, in v;hich anxiety rast be eliminated to eliminate phobic behavior, is questioned here. This e'-qjlanation requires inferences as to unobservable interral processes vMch have not been experimentally established (Breger and I'cGraugh, I965). This present study concerns an attempt to show that phobic beha,vior can be treated effectively by operant methods, concerned only with bringing the subject's observable behavior under stiEulus control, without attempting to reduce or eliminate internal anxiety. This bears on the question of the necessity of reciprocally inhibiting anxiety in treating phobic beliavior. The first hypothesis is that an opera.nt treatment procedures presented within the frajnev/orl: of the technique of desensitization, v;ill result in significojitly less resistance to extinction compared to the disuse or controlled rest condition. Previously an alternate explanation vas offered of the desensiti- zation process in terns of oper?^t principles rather than in terms of classical conditioning theory. Such an alterriite explanation v;ould find support if an operant procedure proved to be a more effective treataent procedure than a desensitization procedure in vhich operant processes were minimally operative, i.e., restricted to a continuous and simultaneous presentation of revfard with the presentation of the CS (so tiiat coni'rontation v;ith the CS is possibly rev/arded). The second h^-pothesis is that an operant treatment procedure, presented within the fraineworl: of desensitization, v/lll result in significantly less resistance to extinction compared to adesensitization procedure, in which operant procedures are minimally involved. 12 It is assumed that these treatment procedures can be applied to the previously conditioned phobic behavior of animals in the follov;- ing v:ay. First, using a 500 Hz. tone as the CS, a conditioned avoidance response to shoclc (UCS) can be established. This response can then be treated by using the progressive approximations method in presenting the CS without the UCS, starting vjith an 8,000 Hz. tone which does not evoke the avoidance response, then progressing tc the original CS (500 Hz. tone). Such progressive presentations of the CS, in hierarclrial arrange- nent frora veekest to strongest item, can then be combined with either a desensitizatioii treatment or an operant treatment. In the desensiti- zation treatraent the hierarchially arranged conditioned stinrali can be gradually introduced in increasing approxination to the original CS while the animal is eating. The various responses involved in this eating behavior should serve to reciprocally inhibit the anxiety aroused by presentation of the CS tone. In the operant treatnent the food is presented in a way such that obtaining it and malting an avoidance response are mutually exclusive. In this operant procedure, obtaining food requires that the animal inhibit his making an avoidance response, making instead various other responses which are competitive with the avoidance response, for increasing periods of time. Both of these procedures can be called effective treatment procedures only if they result in significantly less resistance to ex- tinction than the disuse or controlled rest conditions. Thus, the third b^-pothesls is that the desensitization treatment will also result in significantly 1g-=5s resistance to extinction compared to the disuse or controlled rest conditio:).. METHOD Subjects Thirty experimentally naive, initially 80 days old Long-E^ans male rats were used as subjects. These 30 rats were selected from a gro::i.p of k-Z on the basis of their overall performance during the Acquisition Phase. Selection v:as accomplished by taking out the four fastest a2id eiglit slov.'est rats in terns of average reaction tins bet'.;cen CS onset and CH or hurdle jumping. In the middle of this study three rats died, leaving data on Z? animals (see discussion of matching S_s in H^catinint section belo.^'). Animal Maintenance Each ani-Tal was separately housed in a hanging, letal C2,^q In a controlled tenperature environment. ¥ater was given ad ]ib in all phases of the experiment. Food vas presented ad lib for five days prior to experimentation and then the animals were reduced to 30 per cent of their normal ;;eight. Normal weight v;as determined oj taking the median of the daily weights over th.e last three days of this five day pre-experinenlal period. After normal bcdy veiglit v;as dcterainGd the ajiimals went on their diet for tv/o djajs prior to beginning acquisition training. They were maintained at 80 per cent of normal body weight during the Acquisition and Treatment Pha.se3 of the experi- ment, but were again placed on an ad lib food schedule during the Extinction Phase of the exoeriment. Tlie rationale for placing the 13 14 animals on an ad lib focd schedule during the extinction trials is presented in the Discussion section. All animals were fed each night at the end of the experimental work^ Purim Laboratory Chow was used. Apparatus The wooden shuttle box was painted black and had interior dimensions of 2''i inches long by 6 inches wide by 18 inches high. The floor consisted of k6 copper alloy bars spaced one-half inch apart. The grid floor was divided in half by a four and one-half inch high wooden hurdle which v;as in place at all tines. Only one end of the floor was wired for shock. This saaie "hot" end also contained a food tray which could be pushed into the side of the box pnd which v:as centered betv.'eoii the hurdle and the end of the box. During the Treatment Phase, 20 mg lloycs food pellets were presented by sliding this tray into the bo/.. The empty tray was kept out of the box, the end of the tray covering the box tray opening, during the Acquisition and Extinction Phases. A plexiglass sheet covered a rectangular opening on one side of the box and permitted full observation of S^Sj behavior. The other side of the box contained numerous one-quarter inch holes to permit sound to enter from an adjacent speairer. The shock for the grid was delivered by a Lehigh 7alley Electronics Shock Generator and Scrambler unit which reversed the polarity randomly aniong the 23 grid bars on the hot side of the box. Level of shock was set at one and two-tenths milliapperes (D.C.) as measured by the Simpson meter on the shock unit. Iji AR^x spealier was used adjacent to ons side of the shuttle box with the woofer nsar the "hot" end of the tox. The tones were supplied froa a Keulett-Packard, model 200AB audio Oscillator. Tones vere aeasured ly a General Radio Coicpany Sound-Level Meter, type 1551-B, inside of the shuttle hox with background noise at a 35 db level. A Hunter interval timer was used to measure event duration to the nearest hundredth of a second. Manually operated switches controlled the shod:, tone and timer. V^hen appropriate the switches fro^i the tone generator could be yoked to the timer to measure the duration of the tone. The miming ticier was also used to measure food presentation duration. Procedure Acquisition Phase rurin^ this Phase each of the 42 rats received 20 trials of acquisition training for each of five consecutive days. On the first trial of each day the animal was placed in the hot end of the shuttle box and allowed to roam for a period of two minutes. The 500 Hz., 90 db tone (the original CS) was then turned on if the animal was in the hot end of the box. If the animal was not in the hot end after the two minute period then he was gently placed there. After the CS tone h3.d been turned on for a period of ten seconds shock was delivered. The CS tone (and shock if turned on) was immediately terminated and time recorded as scon as the an.icial jijmped, withdrawing all contact from the hot grid floor. In effect then the animal quit receiving both the 16 sliock and the tone as soon as he jumped. He did not receive shock prior to ten secon'^.s from the tone onset. The latency "bctvfeen the onset of the CS tone and the jumping or avoidance rci-ponse was recorded to the nearest hundredth of a second. Recording of latency was accomplished hy having the CS tone duration switch yoked to the timer so that the duration of the CS tone (v/hich terr'.inated v/ith ^s jumping clear of the hot grid floor) was timed. A latency of ten seconds or greater indicated that the animal did not jump lofore receiving shock. On trials follov/irig the first trial of each day during the acquisition training, the animals \/ere allowed 20 seconds in the "cold" end of the shuttle toz following hurdle jyjnpir>>g and CS termina- tion vhich ended a trial. They did not receive the CSj marking the beginning of a nev; trial, until at least ten seconds aftc-r "being placed hack in the hot end of the "box. Animals jumping vitMn ten seconds of "being placed in the hot end of the "box to "begin a new acquisition trial did not receive either the 03 or the UC3 (shock) but were gently placed "back into the hot end to "begin the trial again. Following the 20 daily trials each animal v;as weighed, returned to his ce^e and fed an amount of Purina La"boratory Chov; "based on the deviation of his weight from 80 per cent of his normal weight. By the fifth day of training, all animals reached the acquisition criterion by hurdle jumping at least 7^ per cent of the time before the dui'ation of the CS reached ten seconds (thus avoiding the shock). 1? Hatching Folloying the fivs days of acquisition training all i|2 animals vere ra_nked according to their overall average reaction time (betv/een the CS onset and jumping behavior). The four animals with the fastest reaction tiaes as well as the eight slowest reacting animals were dis- carded from further experimentation. Three groups of ten animals each vere then drawn for the tv.'o treatment and control groups. The Control group vas comprised of the following ranked aninals: 1, 6, 8, 12, 14, 16, 20, 22, 27 and 30. The Operant treatment group was sade up of animals v;ith these ranked numbers: 2, A"-, 9$ 10, 15, 1?, 21, 23, 25 and 29. The Deseusitization treatnsnt group vas coir:?csed of the follov;- Ing rank numbered aninals: 3, 5, 7, 11, 13, 18, 19, 2^1, 26 and 28. The average reaction time over the 100 trials of acquisition training, computed for ea-^h group of Icn rats aft^r the anioials were formed into the three groups, v,'as as follGv;s: Control group, k.33 seconds; Operant group, 4.94' seconds; and the Desensitization group, 4.94 seconds. The groups of animals v.-ere ihus thought to be well matched in terms of the overall average time it took then to jump the hurdle upon onset of the CS during acquisition training. On the third day of treati:.ent three of the experimental animals, one from each of the three groups, were founl dead. Although no autopsy vas performed the presence of blood traces near the external urinary region of these animals suggested a urinary tract infection. The remain- ing 2? S^s survived the experiment, apparently in good health. The elimination of these three animals did not appear to upset the previous matching. The ranl-ed positions of these S_s, in terms of overall rraction time in the acquisition trials, were 17, 18 and 20. 10 The average reaction times of the three groups changed as fol],ov;s wltl; the eliDination of the three animals: Control group, dropped fron 4.93 to 4.90 seconds; Operant group, reiaained the same at 4.9^ seconds; and the Desensiti7ation group, dropped from 4.9^ to 4.93 seconds. Since matching appeared to he maintained the study progressed as scheduled v;ith the remaining 2? S_s, Treatment Phiise Treataent tsgan the day after conpletion of the acquisition trials. Both the Operant and the Desensitization treatment gi-oups received equal exposure to the CS stimuli components during treatment. Both groups also received equal exposure to the food pellets used in treatment. The tonal hierarchy presented during treatment in accord- ance with ths method of progressive approximations consisted of the following five tones! an 8,000 Hz., 66 dh tone presented for two seconds; a 4,000 H2., ?2 dh tone presented for three seconds; a 2,000 Hz., 78 db tone presented for four seconds; a 1,000 Hz., 84 dh tone presented for five and four seconds; and a 50O Kz., 90 dh tone (original CS) presented for four, six and seven seconds. These tones were pre- sented over the five dsy Treatment Phase period in a total of 84 treat- ment trials. Food was also presented. The timing of the food presenta- tion in relation to the tone presentation defined and distinguished the Operant and Desensitization treatment groups. Table 1 provides a detailed description of the tonal hierarchy and the presentation of these CS tones In relation to food pellet presentation in the two treat- ment groups. 19 1-f o CD -C) f:i o o o .-^ f:; O rj c- + q3 f-i •O' C> E-^ O f-1 o o p; Ps &-. o 'i t: CJ ■♦-•' Ti d o o 10 O r-i C P=H o o cd (is E-. Qj a « o O r-H E-. < t-j-c! t> c r! c it! o a> ■r^ ;U i-i +^ W CD c3 ei t> f 1 ^^B r-t ri •r^ fH EH >? c5 « I O w C0"0 CO O ^ O O ,0 O ,o O w O «n T-H vr\ 20 -Cf 03 •ci ±^% 227-229. ~" — . Winer, B. J. Statistical principles in erperimental design. Nev; York: McGrraw-HiTr, i'9c2. WoIdc, J. SroeriEental neurosis as learned behavior. British Journal of Psycholog:--, 1952, 43 > 192-206. Wolpe, J. Psychotherapy by reciprocal inhibit io.n. Stanford: Stanford Press, I95B". Wolpe, J. The systematic desensitization treatment of neuroses. Journal_ of Nervous and Mental Disease, I96I, 1^2, 139-203. Wolpe, J. and Lazarus, A. Pehavior therapy techniques. Nev/ York: Pergaraon Press, 19^7 ~^ Wolpin, M. and Raines, J. Visual imagery, expected roles, and extinction as possible factors in reducing fear and avoidance behavior. Behavior Research and Theraw, 1966, 4, 25-37. J^PENDICSS 55 iPPE^IDIX A EXTINCTION PrlASE: DAILY AVERAGE UTENCY RECORDED TO THE NEAREST FUl'IDREDTH OE A SECOND AIID SU^-CISD ITOiBER (GIVSI PAREHTHETIC.lLLl) OE AVOIDANCE RESPONSES MADE BY THE CONTROL GROUP OF TEN TRLA.LS OVER FIVE DAYS Days Animals 1 2 3 4 5 1 10,85 (8) 11.32 (8) 9.01 (9) 12.38 (9) 5'S(^ (10) 2 4.^?. (10) 5-3 (10) 8.73 (9) 8.76 (9) 6.46 (9) 3 5.69 (9) 1.82 (10) 5.S8 (10) 3.43 (10) 1.94 (10) k 7.58 (10) 9.72 (10) 10.40 (10) 9.74 (9) 7.88 (iO) 5 5.79 (9) 7.98 (10) 3.93 (10) 7.04 (10) 2.48 (10) 6 6.89 (9) 12.7^ (6) 12.50 (6) 8.53 (10) 12.22 (9) 7 9.75 (8) 14.01 (6) 6.41 (10) 12.89 (10) ^'S5 (9) 8 10.57 (8) 14.65 (8) 13.0? (5) 10.82 (8) 11.10 (6) 9 6.33 (10) 9.86 (10) 9.90 (9) 8.82 (9) 6.60 (10) 56 APPEi^IDIX B EXTINCTION PHASEt DAILY AVE.RAGS UTENCY RECORDED TO TPJ) KE.1PJST HUIIDREJJTK 0? A SECOND AIJD SUIIMSD NUMBER (GIVEN PARSJTHETICAI-LY) OE AVOIDANCE REfiPONSES M'lDE BY TKpI OPERA^^T GROUP OE TEN TRIALS OVER EIVE DAYS Days Animals 1 2 3 4 5 1 1?.33 (2) 12.33 (6) 11.25 (8) 16.23 (4) 10.80 (7) 2 10.68 (8) 9.62 (8) 9.1''^ (10) 11.24 (8) 6,60 (10) 3 10.11 (9) 12.07 (7) 6.40 (10) 11.36 (9) 8.26 (8) ^ 18.70 (1) 15.01 (7) 8.76 (9) 9.81 (8) 11.3^ (8) 5 15.59 (5) 8.98 (8) 8.33 (9) 10.08 (8) 7.53 (10) 6 20.00 (0) 18.42 (3) 10.68 (?) 10.79 (7) 8.20 (7) 7 20.00 (0) 20.00 (0) 20.00 (0) 20.00 (0) 20.00 (0) 8 13.50 (7) 13.22 (7) 12.96 (7) 8.12 (10) 12.98 (5) 9 11. 2^^ (7) 13.90 (7) 13.41 (6) 13.19 (6) 14.69 (6) 57 APPE:roix c EXTINCTION TRIALS; DAILY AVER/.GS LATENCY RECORDED TO TKE NEAREST HUNDRIIDTH OE A SECOND AI'ID SiJK.iED NUI-IBER (GIVEN PAHEilTHETI CMXY ) OF AYOIDMCE R2^P0NSE5 MADE BY THE DiSKNSITIZATION GROUP 0? TEN TRIALS OYER FIVE DAYS Days Animals 1 2 3 4 5 1 13.17 (4) 17.69 (2) 9.01 (9) 12.38 (7) 18.90 (1) 2 19.57 (1) 16.88 (8) 12.30 (6) 18.55 (2) 18.33 (1) 3 19.53 (1) 8.59 (7) 13.60 (6) 11.02 (7) 10.86 (7) 4 19.53 (1) 17.05 (4) 15.71 (5) 14.06 (5) 8.74 (C) 5 20.00 (0) 2O0OO (0) 12.36 (8) 19.30 (1) 13.10 (4) 6 20.00 (0) 19.40 (?.) 14.20 (5) 16.34- (4) 12.00 (6) 7 7.69 (9) 5.89 (10) 7.16 (10) 11.59 (8) 8.72 (9) 8 15. t9 (3) 20.00 (0) 17.53 (2) 11.12 (7) 15.00 (4) 9 9.61 (7) 8.00 (9) 5.88 (10) 8.27 (8) 14.69 (9) BIOGRAPHICAL SKETCH Arthur H. VJells, Jr., V7a«> "born on October 9, 1939, at Birming- han, Alabacia. He mo^ed to Florida in 19^9, where he attended puhlic schools. He v;as graduated from Ft. Lauderdale High Scliool in June, 1958. In August, 1962, he received the degree of Bachelor of Arts froa the University of Florida. Dyeing the s^oa^er of I96I, he worked with maladjusted children under David Wineman while attending the University of Michigan. In August, I965, he received his Master of Rehahilitation Counseling degree from the University of Florida. After working for one year in MitM, Florida; as a Reh-ahilitation Counselor, he returned to the University of Florida to work to\,'ard his doctorate in p-ychology. During his graduate studies in prycholog;.', he was a Vocaticn-il Rehahilitation Administration fellow. In Au^st, 1969, he completed a one year's internship in clinical psychology at the J. Hillis Miller Health Center. Since Septemher, 1969, he has worked as a psychologist at the Northeast Florida State Hospital in Macclenny, Florida. He was honoraoly discharged from service in the U. S. Air Force. Arthur M. Wells is married to the former Ar^stasia E. Lawlor. He is a menher of Psi Chi a.nd Phi Kappa Fhi. 58 Thri.s discertation vas prepared under the direction of the chairfflan of the candidate's supervisory comiiittee and has been approved "by all inefflhers of tliat coa-'nittee. It v/as sutmittcd to the Dean of the College of Arts and Sciences and to the Graduate Council, and uas approved as partial fulfillaent of the requirements for the degree of Doctor of Philosopiiy. March, 19?0 Dean, College of Arts and Sciem; Dean, Graduate School Supervisory Coiniiitteej