3>4 TtefUVlVttCL INSECT DISPLAYS 330.4 C212 P1032 1958 c.3 CANADA DEPARTMENT OF AGRICULTURE Publication 1032 October 1958 m Tte/t&Junty INSECT DISPLAYS By A. A. WOOD Entomology Laboratory Chatham, Ontario Science Service • Entomology Division CANADA DEPARTMENT OF AGRICULTURE EDMOND CLOUTIER, C.M.G., O.A., D.S.P. QUEEN'S PRINTER AND CONTROLLER OF STATIONERY OTTAWA, 1958 Price $3.00 54899-0 — 1 iC-23680-IO:53 Price $3.00 Cat. No. A43-1032 Available from the Queen's Printer Ottawa, Canada CONTENTS Page ILLUSTRATIONS iv PREFACE vii COLLECTING AND REARING INSECTS 1 PRESERVING IMMATURE INSECTS Dry Mounts 2 Liquid Preserving 4 PRESERVING ADULT INSECTS 5 COLLECTING AND PRESERVING PLANTS Collecting 6 Mounting 7 Preserving Green Colors 7 PLASTER MOLDS Plaster — characteristics and uses 8 Preparing Molds 9 Plaster Slabs — preparation of 14 FLEXIBLE MOLDS Latex Molds 14 Gelatin Molds 19 METAL MOLDS: FOR CASTING LEAVES IN PLASTIC Constructing the Mold 19 Casting in Sheet Plastic 21 CASTING IN WAX Fruit and Vegetables 22 Leaves and Petals 26 Larvae 28 Plaster Casts 28 CASTING IN PLASTICS The Walters Method 28 Coldpress Casting of Leaves and Petals 34 Plastic Material for Cold Molding 35 Casting Foliage for Miniature Groups 36 Embedding in Plastic 36 PAINTING AND ASSEMBLING Assembling Plant Parts 37 PRESERVING CONIFEROUS FOLIAGE 40 BUILDING INSECT DIORAMAS 48 Example: Exhibit of Wheat Stem Sawfly — details of preparation 42 Animated Models 47 AIRBRUSH TECHNIQUES 47 BUILDING INSECT DIORAMAS 48 DISPLAY UNITS AND LABELS Display Units 50 Labels 54 iii Page SHIPPING 59 FORMULAE Preserving Larvae Dry 60 Preserving Larvae in Liquid 61 Preserving Adults 61 Alcohol: Uses and Diluting 64 Soldering 65 Preserving Leaves, Stems, Fruit and Vegetables in Liquid 65 Preserving Red in Fruits and Vegetables 66 Preserving Color in Plants for Mounting 67 Separators for Plaster Casts 68 Separators for Wax Casts 69 Base Construction (Maches) 69 Thinners for Airbrush Oil Painting 70 Preserving Natural Soil Units for Bases 70 Methods for Casting in Plastic 70 EQUIPMENT AND MATERIALS Laboratory Facilities 71 Mold Making 71 Casting in Wax 72 Casting in Plastics 73 Art Work 73 Assembling Exhibits 74 Collecting Insects 75 Inflating Larvae 75 Collecting Plants 75 Preserving Plants 76 Miscellaneous 76 APPENDICES A — Sources of Equipment and Materials 77 B — Supply Houses 79 C — References 80 D— Glossary 82 E — Index 85 ILLUSTRATIONS Page COVER — Cabbage insects, Plant reproduced in cellulose nitrate. COLLECTING AND REARING Figure 1 — Cage for rearing larvae 7 Figure 2 — Mounted herbarium specimen with label 7 PLASTER MOLDS Figure 3 — Left, potato tuber in clay with dam. Right, first section with holes drilled for keys 10 Figure 4 — Left, mold ready for second pour. Right, mold ready for third pour 10 Figure 5 — Open mold showing pouring opening and holes for holding staple... 10 Figure 6 — Tin forms for leaf mold 13 Figure 7 — Plaster mold of leaf 13 RUBBER MOLDS Figure 8 — Rubber mold in plaster packet showing treatment of undercut in a sugar beet 15 iv Page Figure 9 — Exhibit of injuries to sugar beets. The lower model was cast hollow from the rubber mold illustrated in Fig. 8. (Displayed in Science Service Building, Ottawa.) 15 Figure 10 — First stage in preparing a rubber mold. Left, tin on wood base with wires for mounting tuber. Right, tuber mounted ready for applying latex 16 Figure 11 — Left, rubber mold on tuber embedded in clay. Right, ready for first plaster pour for the mother mold 16 Figure 12 — Left, mold ready for second pour. Right, with key holes for third pour 17 Figure 13 — Left, plaster jacket complete. Right, rubber mold being removed from the tuber 17 Figure 14 — Rubber mold of cabbage head filled with plaster for storage to prevent shrinkage. Plaster jacket in sections 17 Figure 15 — Plaster casts showing one enlargement from a rubber mold 17 METAL MOLDS Figure 16 — Impression in molder's sand from plaster mold of leaf 20 Figure 17 — Metal mold complete 20 Figure 18 — Metal mold being smoked to form a separator for the second pour 21 CASTING IN WAX Figure 19 Exhibit of potato diseases. Tubers cast hollow from rubber molds. (Displayed in Science Service Building, Ottawa.) 24 Figure 20 — Exhibit of insect and other injuries to potato tubers. Cast hollow from plaster molds. (Displayed in Science Service Building, Ottawa.) 24 Figure 21 — Exhibit of the apple maggot. Cast hollow from plaster molds. (Displayed in Science Service Building, Ottawa.) 25 Figure 22 — Exhibit of some pests of the tomato. All in wax. (Displayed in Science Service Building, Ottawa.) 25 Figure 23 — Wire and cotton on leaf mold ready to cast in wax 26 Figure 24 — Wax leaves. Left, as leaf comes from the press. Right, the edges trimmed 26 Figure 25 — Rolling to thin the edge of a wax leaf 27 Figure 26 — Wax larvae cast in plaster mold 29 CASTING IN PLASTIC Figure 27 — Upper, plaster waste mold speckled with ink to aid in applying even coats of liquid plastic. Lower, plastic cast on the mold 30 Figure 28 — Exhibit of cabbage insects. In cellulose nitrate. (Displayed in Entomology Laboratory, Chatham.) 32 Figure 29 — Exhibit of the eastern tent caterpillar. Foliage in cellulose nitrate; branch in wax; larvae inflated empty. (Displayed in Forest Insect Laboratory, Sault Ste. Marie, Ontario.) 32 Figure 30 — Exhibit of the spinach leaf miner. In plastic. (Displayed in Science Service Building, Ottawa.) 33 Figure 31 — Exhibit of the tomato hornworm on tobacco. Foliage in cellulose nitrate; eggs and larvae in wax. (Displayed in Science Service Building, Ottawa.) 33 Figure 32 — Rubber mold for casting leaves in liquid plastic 35 Figure 33 — Cold-press casting in plastic. Plaster mold, plasticine, and cast leaf 35 PAINTING AND ASSEMBLING Figure 34 — Compound leaf. Upper, potato leaflets soldered to central wire. Center, leaf stalk modelled in wax. Lower, stem and midribs masked, for spraying leaf blades 38 Figure 35 — Exhibit of insects on a hill of potatoes. Prepared in wax by casting and modelling. (Displayed in Entomology Laboratory, Chatham.) 39 Page PRESERVING EVERGREEN FOLIAGE Figure 36 — Exhibit of the European spruce sawfly. Preserved foliage colored. (Displayed in Forest Biology Laboratory, Fredericton, New Brunswick.) 41 BUILDING ENLARGED MODELS Figure 37 — Exhibit of the wheat stem sawfly. Models enlarged six times. In cellulose butyrate. (Displayed at Permanent Canadian Exhibi- tion, London, England for some time after 1949.) 43 Figure 38 — Tool for scribing striae on enlarged plastic models of wheat stems 44 Figure 39 — Left, enlarged pattern of section of wheat leaf. Right, model of section in plastic 46 Figure 40 — Model of female of the wheat stem sawfly in plastic 46 AIRBRUSH TECHNIQUES Figure 41 — Gauge for liquid carbonic acid gas outfit 48 BUILDING INSECT DIORAMAS Figure 42 — Plan of diorama unit. Left, proportion of the base. Right, end elevation 49 Figure 43 — Tools for modelling paper-based leaves. Upper, veining tool. Lower, embossing tool 50 Figure 44 — Injury by the clear-winged grasshopper to Marquis wheat, and egg bed in sod. Plants in cellulose nitrate. (Displayed in National Museum of Canada, Ottawa. ) 51 Figure 45 — Grasshoppers emerging from sod, and control in wheat with poisoned bait. Plants in cellulose nitrate. (Display in National Museum of Canada, Ottawa. ) 51 Figure 46 — Plans of walnut display cases with glass tops 52 Figure 47 — Museum display unit 54 Figure 48 — Special display unit for a small exhibit 55 Figure 49 — Method of assembling plate glass in unit shown in Fig. 48 55 PREPARING PHOTOGRAPHIC LABELS Figure 50 — The Coxhead-Liner photo-lettering machine 57 Figure 51 — The Coxhead-Liner Typemaster 57 Figure 52 — Gothic copperplate in the various sizes used 57 Figure 53 — Lydian cursive, actual size and reduced 57 Figure 54 — Proof of monotype, actual size 57 Figure 55 — Completed caption ready for photocopying 57 Figure 56 — Completed caption as used in exhibits 57 SHIPPING Figure 57 — Shipping case with buffers of foam-rubber 58 EQUIPMENT Figure 58 — (1) Scissors, (2) forceps, (3) scalpel, (4) combination knife, (5) wax spatulas, (6) dental probe, (7) spoon and seeker, (8) dis- secting needle, (9) holder for razor-blade splinter, (10) Swiss pattern files 72 Figure 59 — (1) Proportional divider, (2) caliper, (3) micrometer caliper, (4) dividers, (5) ruling pen, (6) swivel stencil knife, (7) flexible spatula, (8) cutting forceps, (9) long-nosed cutting pliers 72 Figure 60 — Hand-press for casting leaves 73 Figure 61 — Tray of brushes in solvent for casting in liquid plastic; the stoppers are fixed to the handles 73 Figure 62 — Airbrushes. Left, Wold. Center, Thayer and Chandler. Right, Paasche 74 Figure 63 — Electric drill with flexible-shaft and grinder 74 Figure 64 — Cautery set and glass cannulae with spring clips for inflating larvae 76 Figure 65 — Plant press with driers and ventilators 76 vi PREFACE This manual outlines the various museum methods and procedures that may be adapted for preparing entomological exhibits — a phase of exhibition in museums that has not received a great deal of attention up to now. Exhibits of insects, because of their small size, must be displayed in small units for viewing at close range; the need for accuracy in all details presents an important problem. Insects and plant sections must be so pre- pared that each appears as a replica of the original. Exhibition work in this field is highly specialized and requires wide knowledge of art, entomology and botany, as well as many specialized techniques. Accuracy of detail and lifelike quality are keys to successful preparation of exhibits. The critical personal touch is necessary to remove the stamp of mass production and raise them to works of art. Good mech- anics alone is not enough; originality and inventiveness in tools, materials, and design are needed constantly. Many museum techniques developed through the years, especially those involving the preparation of herbaceous units, may be adapted for preparing insect displays. Success, however, depends on the ability of the worker to employ these methods skillfully. The author gratefully acknowledges the help and encouragement of the following: Mr. Ramon Bermudez, and Mr. F. C. Schmid, Academy of Natural Sciences, Philadelphia, Pa., the former for modelling and painting wax casts, the latter for group building, backgrounds, miniature models, paper leaves, and diorama lighting; Dr. James Chapin, Messrs. C. M. B. Cadwalader, J. L. Clark, C. E. Olsen, and G. Petersen, and Miss Alice Gray, American Museum of Natural History, New York, N.Y., for general museum procedure, field collecting and care of specimens, diorama tech- niques, celluloid casting, preparing plants in plastic paper, and coloring wax casts; Messrs. Brayton Eddy and Earl Chase, Bronx Zoo, New York, N.Y., for field collecting, feeding, and exhibiting living insects; Messrs. H. H. Clement, G. A. Linck, O. F. von Fuehrer, and Mrs. O. F. von Fuehrer, Carnegie Museum, Pittsburgh, Pa., for useful materials and techniques in group building, enlarged models, uses of latex in museum techniques, wax casting and modelling; Messrs. Milton Copulos, W. J. Gerhard, J. H. Quinn, Emil Sella, and L. L. Walters, Chicago Natural History Museum, Chicago, 111., for molding and casting fruit, collecting and preserving insects, rubber molds and plaster casts, plaster and metal molds, plastic casting and assembly; Messrs. D. M. Blakely, James Carmel, and Walter Nickell, Cran- brook Museum, Bloomfield Hills, Mich., for forest dioramas, enlarged models, reinforcing base accessories; Messrs. C. E. Johnson and Clyde Patch, vii National Museum of Canada, Ottawa, Ont., for plaster molds, wax casts, and group construction; Messrs. C. R. Aschmeir, W. L. Brown, and E. G. Laybourne, National Museum, Washington, D.C., for plaster molds and wax casting, group technique, and plastic casting; Messrs. H. Brooks, E. Keen, and T. B. Pitman, Petersham Forestry Museum, Petersham, Mass., for back- ground painting, the mechanics of miniature tree techniques, and the prepara- tion of miniature forest groups; Messrs. T. B. Kurata, E. B. S. Logier, Archie Reid, T. M. Shortt, L. L. Snyder, and L. Sternberg, Royal Museum of Zoology and Palaeontology, Toronto, Ont., for enlarged models and painting, airbrush technique and color blending, rubber molds and plastic casting, gallery display, plaster molds and plaster casts, Dr. D. L. Gamble, Ward's Natural History Establishment, Rochester, N.Y., for formulae; Dr. W. P. Haynes, University of Illinois, Urbana, 111., for formulae; Dr. Carl D. Clarke, University of Maryland, College Park, Md., for flexible molds and formulae; Drs. A. A. Granovsky and G. H. Vacha, University of Minnesota, St. Paul, Minn., for formulae for preserving fruit and vegetables; Dr. R. L. Post, North Dakota Agricultural College, Fargo, N.D., for formulae; Dr. Alvah Peterson, Ohio State University, Columbus, Ohio, for formulae for preserving insects; Mr. John Gates, Science Service Laboratory, London, Ont., for taking photo- graphs; and Mr. G. H. Parker, Bio-Graphic Unit, Science Service, Ottawa, for contributing the section on preparing labels. A. A. WOOD vm COLLECTING AND REARING INSECTS In preparing an entomological exhibit, an insect net and killing bottle are essential equipment. Beirne (1) gives detailed directions for collecting and mounting insects for systematic collections. However, there will be little need for standard mounting boards; insects used in exhibits are usually mounted in lifelike positions. Sometimes it may be necessary to rear larvae in the laboratory; netted specimens of adult Lepidoptera, for example, often lose scales and are not satisfactory for this type of display. This is one case where newly emerged specimens should be used. Bring the larvae into the laboratory alive with a supply of their host plant. Lantern breeding cages (Fig. 1.) for small larvae can be made using sheet galvanized metal for the base and a shallow metal can with a friction top with a hole in the center for inserting plant stems in water. A lantern globe fits into a flange about f-inch high around the top of the metal can. Enclose the top of the glass with cheesecloth or screening. This type of cage will hold evergreens fresh for a week (see Fig. 1 p. 7). Many larvae may be reared by placing foliage in large vials and loosely plugging the openings with moist cotton. Almost any type of clean container will do if it will confine the larvae, hold the food plant fresh, retain moisture, and furnish sufficient space for proper expansion of wings. Insects that pupate in the ground will need a layer of soil in the cages. Refrigeration saves time in rearing insects and especially helps by keeping food plants fresh. It retards surface drying, the main limiting factor in keeping a supply of food. Fresh deciduous or coniferous foliage, layered between sheets of wet paper towelling and placed in a covered crisper, will remain in good condition for a week or two at temperatures a few degrees above freezing. Larvae of many insects held for making molds, inflating, or coloring, can be kept in tight containers under similar conditions of temperature. The last two instars of lepidopterous and hymenopterous insects stand refrigera- ion better than younger larvae. Peterson (27) describes various breeding cages and traps for insects. PRESERVING IMMATURE INSECTS Preserving insect larvae has always been a disappointing procedure. Loss of color and form resulting from the various formulae used have dis- couraged most workers from using larvae in exhibitions. Some larvae when inflated hold their color very well but the form of most is undesirable when conventional methods are used. 1 There are improved methods for preparing artificial insects but pre- served specimens must still represent many forms in exhibits. Following are some approved methods for preserving larvae. Dry Mounts With Formulae 1 to 3 (p. 60)* specimens may be mounted dry after processing. Newly hatched larvae are difficult to preserve dry because shrinkage is great; Formula 2 causes less shrinking than Formula 1. Larvae should be preserved dry for exhibits only if it is impossible to make wax casts. If Formula 3 (p. 60) is used, place larvae over one centimeter long in cold water over a slow fire and remove the container when the water reaches the boiling point. After the water cools to room temperature, puncture the larvae in several places with a fine needle so that alcohol solutions will penetrate. Do not boil smaller larvae or pupae but place them in water at the boiling point and leave to cool to room temperature then pass them through Formula 3. Larvae or pupae over one centimeter long should be left in each of the solutions six to eight days, and smaller larvae or pupae two to six days. Then take the specimens from the turpentine and let them dry on blotting paper; mount as desired when dry. Post (28) suggests the following method for dry-mounting larvae: boil them one-half to one minute in water, remove, and pass through the alcohols. Finally place them in xylol, then pin, and dry. This method preserves Phyllo- phaga larvae better than Barber's method. Scarabaeid larvae should not be boiled too long; they will suddenly deflate and resist all attempts to bring them back to normal appearance. Preserving Insect Eggs — Formula 1 or 2 (p. 60) may be used. When the eggs are thoroughly dry, color tint them using pigment mixed in xylol, turpentine, or white shellac. Do not use water because it will deflate the specimen. Dipping the eggs in a very thin solution of Formula 45 (p. 70) will impregnate them and make them rigid. To preserve tent caterpillar egg masses place the twigs with eggs in an oven at 225 °F. for 20 minutes. When the eggs cool, impregnate them with a thin solution of cellulose nitrate, Formula 45 (p. 70), or Gelva V 15, Formula 12 (p. 63). Cut off the twig near the egg mass. The shrinkage of the twig will allow the ring of eggs to slide off. To assemble for a permanent display, slip the egg mass over the prepared wax twig before it is built up to full size and model wax around it. Large insect egg shells may be preserved by blowing as with birds' eggs. Make a small hole in one side of the egg with a small needle and with a blowpipe — made by drawing a small glass tube to a fine point — expel the contents. A short piece of rubber tubing attached to the end of the glass will make the blowing operation less difficult. Hold the tip of the blowpipe either near the hole in the egg shell or insert it slightly. Air forced into the egg will empty the shell. *The various formulae used for preserving insect and plant specimens are listed together from p. 60 to p. 71 in the back to avoid repetition in the text. All will be referred to in the text by number and page only. Gunder (11) gives detailed instructions for blowing insect eggs using T. M. Blackman's method. One useful point made in this article, is the method of holding the egg while blowing it. If the egg is not attached to something, a minute drop of adhesive can be used to fasten it to a piece of paper. This frees the right hand to operate the blowpipe; the paper with the egg attached is held with forceps in the left hand. Inflating Empty Larval Skins — Living larvae may be used. First cut around the anus, freeing the alimentary canal attachment then squeeze gently near the opening to extrude the viscera; with the forceps, carefully remove all body contents. Place the larva between two pieces of blotting paper leaving the caudal end exposed. Roll the larva gently, pressing at the middle to force the body contents through the anal opening. Repeat the process, pressing at the head until all fat and body fluids have been removed without enlarging the opening in the skin. For all specimens except certain green forms, it is most important to remove all the alimentary canal and contents. Some pale- green larvae hold their color better if all body juices are not removed. To dry the skin, Ross (32) suggests using a 250-watt infra-red lamp with ruby red glass and a nickel reflector clamped on a stand. If the skin is held about 2 inches above the center of the lamp and rotated it will dry without scorching the integument or the setae. This process will not appre- ciably alter the natural color. If you use an inflating oven, heat it with an alcohol lamp. Insert the cannula tip into the anal opening and either fasten it with clips or hold it in place with forceps. If you use spring clips, apply vaseline to the glass tip to keep the skin from sticking. Be sure not to pull the skin too far onto the glass. Maintain just sufficient air pressure in the bulb to extend the skin to natural size. One of the most glaring faults of this technique is over- inflation which causes the segments to separate. Turn the specimen constantly to ensure even drying. When the skin has dried thoroughly remove the cannula. If the skin sticks to the glass, great care must be taken not to tear it; a little moisture may aid removal. After the skin has dried for a day or two, coat the inside with a thin plastic lacquer, Formula 12 (p. 63), injected through the anal opening with a hypodermic needle. Inflating Larvae with Wax — A more recent method of preparing larvae for exhibits consists of inflating the skin with hot wax; it is a modification of the Silver (37) method. It has given better results than the standard pro- cedure above in which the skin is left empty. This method has been used successfully to prepare smooth-skinned species of dipterous, coleopterous, and lepidopterous larvae and some of the sawflies. Empty the skin as described above for inflation. Use larger cannulae made from i-inch glass tubing. Fasten the watch spring clips to the tubing with fine annealed wire. Melt a mixture of 1 part pure white beeswax and 2 parts paraffin wax in a double boiler. Tint the wax with artists' tube colors, blended and thinned with turpentine to match the basic body color of the larva. Draw hot wax into the cannula for half its length. When the wax has cooled, slip the skin over the glass tip and fasten with clips. Attach the cannula to the cautery set. Hold the wax-filled end of the cannula over the inflating oven in such a position that all the wax will liquefy without the larval skin becoming too hot. Regulate the pressure in the bulb to force the wax into the skin, gently filling it to natural size. Then immediately dip the specimen and the glass tip in cold water. Bend the specimen to any desired lifelike position as soon as you remove it from the glass or place it in warm water until the wax softens sufficiently. Do the shaping before the skin dries. Fill the hole left in the wax by the cannula and shape the integument to a natural poise. To pest-proof the skin, treat it with a weak solution of mercuric chlor- ide, Formula 10 (p. 62). When thoroughly dry, dip it in a very thin Gelva solution, Formula 12 (p. 63); this will seal and strengthen the specimen. The skin should not show more than natural glossiness; usually two thin coats give better results than one heavier one. After each dipping, roll the specimen about on porous paper to get rid of excess plastic lacquer. If the skin needs coloring, do this before treating with plastic. Liquid Preserving Specimens processed by the following methods must be kept in liquid preservatives. Kahle's fluid, Formula 6 (p. 61), is used chiefly to preserve larvae permanently. Place the larvae in cold water and bring them slowly to a boil. Leave them over the heat until the body begins to straighten, but not until the segments separate. Allow the water to cool to room temperature before transferring the larvae to the preserving fluid. Craig's method of preserving color in larvae, Formula 5 (p. 61) appears to hold some colors well. A series of fifth-instar larvae of the tomato hornworm was successfully prepared by this method. Post (28) offers the following method for avoiding bubbles in vials. Form a blunt plug of good-quality absorbent cotton by twisting it between the thumb and the forefinger. Saturate the plug with the solution and insert it in the full vial. Fill the space above the plug and insert the cork. The plug should fit the vial snugly. Any bubble formed between the plug and the cork cannot reach the specimen. To make sure corks remain firmly in vials, lay a fine insect pin along the side of the cork as it is pressed in; this allows air to escape. Remove the pin and twist the cork to fill the groove left by the pin. The cork will be held in by the vacuum formed. Preserving Larvae in Gelatin (13) — Kill the specimens by dropping them into boiling water. Then place them in Formula 6 (p. 61) for 12 hours. Wash the material in tap water until all traces of the odor of the fixative have disappeared; this may take 24 hours. Keep in 3 per cent formaldehyde- distilled water until ready to mount in gelatin. Remove the specimens and allow them to dry for a few minutes on a towel. Cut cards to fit the vials and attach the specimens with Duco cement. 4 Insert the mounts in the vials and fill with hot gelatin, which is prepared as follows: Dissolve 5 gm. of bacteriological gelatin in 100 ml. of distilled water. Heat until it becomes clear. Stir in 20 drops of 40 per cent formal- dehyde. The solution hardens slowly. When it has set, cork the vials and seal them with two or three coats of Duco cement. Gelatin mounts are superior to mounts in liquid preservatives because the specimens do not move around in the vials and there is little loss from evaporation. The reaction of the gelatin and formaldehyde produces a per- manent gel that will not melt in hot water. The jelly-like material in the vial acts as a shock absorber for the mount. PRESERVING ADULT INSECTS Adult insects pinned and dried in the usual way will not last long in exhibits. It is necessary to preserve them in such a way that colors will not fade, that the specimens will remain free from pest attack and be strong enough to withstand the vibrations of moving or shipping. A few of the techniques used for preserving in color are briefly noted. Lepidoptera — Large-bodied moths should not be dried and mounted in exhibits without suitable processing. They are subject to attacks from pests and become greasy. To avoid greasiness, cut a slit along the underside of the abdomen and clean out thoroughly, removing as much fat as possible. Swab dry with bits of absorbent cotton and apply arsenical soap, Formula 11 (p. 63), to the interior, working it up through the thorax. Insert a manikin of cotton, filling the cavity. Mount the specimen in a lifelike posi- tion and allow it to dry. When the wings are in a position so that the bottom sides will not show in the exhibit, coat the underside of the specimen with white shellac to strengthen it; pay particular attention to the bases of the wings and the undersides of the body, head, and leg attachments. Coat the antennae with thin white shellac or plastic lacquer and apply a second coat to the bases. To reduce fading of colors in moths and butterflies, paint the scales. It is better to use fresh specimens but it is possible to restore faded ones this way. You can probably do the finest work with an airbrush. Mount the specimen on a base with supports against the undersides of the wings to protect them from the blast of the airbrush. Use masks to work out the color pattern, and spray. Claude E. Johnson, formerly preparator in the National Museum of Canada, prepared a polyphemus moth by this method. This specimen has held colors well although exposed to light for several years. You may also brush in colors using the transparent oil colors for slide and photograph tinting. Thin the pigment with xylol and allow it to run into the scales. Orthoptera — The following method can be used to preserve grasshoppers satisfactorily. Immerse living specimens in Formula 8 (p. 62) for one day. Cut along one lateral abdominal suture and remove all viscera with fine forceps. Insert wads of absorbent cotton and rub the walls of the cavity and up into the head until the swabs come away clean and dry. Brush thinned arsenical soap, Formula 11 (p. 63), over all of the interior. Insert a cotton manikin to fill the cavity. Close the incision, mount in the desired position, and let it dry thoroughly. Dip in a very thin Gelva solution, Formula 12 (p. 63), at least twice which will impregnate and hermetically seal the specimen. Liquid cellulose nitrate or acetate may be used, but has not the penetrating quality of Gelva. This method holds the colors and greatly strengthens the specimen. For green katydids, place freshly killed insects in Formula 8 (p. 62) for one day and then treat them in the same manner as grasshoppers. Coleoptera — With large beetles, remove the viscera through an incision in the underpart of the abdomen. Insert absorbent cotton saturated with arsenical soap, Formula 1 1 (p. 63). When dry, dip the specimen in thin Gelva solution, Formula 12 (p. 63), to impregnate and seal. Small Soft-bodied Insects — Use Formula 2, (p. 60), for insects whose abdomens will remain plump. The method works well for larvae and midges and other small, soft-bodied adults that are to be mounted in lifelike positions. COLLECTING AND PRESERVING PLANTS The reproduction in wax or plastic of insect injury to plants often makes it necessary to collect insect-injured plant series to be used as patterns in copying the injury on artificial reproductions. The natural material may be mounted as scientific specimens on botanical mounting sheets, or small twigs and leaves may be mounted on filing cards and completely covered with scotch tape. Collecting Choose plants typical of the species. Bend tall specimens several times, or cut out sections of the stem to conform to the size of the mounting sheets. Always leave roots attached. Be careful with the artistic arrange- ment of plants before drying, so that the appearance of the finished mount will be satisfactory. Arrange the plant in the press as soon after collecting as possible. Fold a sheet of porous paper, such as newsprint, and place the plant inside, paying particular attention to arrangement of leaves and flowers. Place the folded paper, with the plant inside, between driers in the press. Leave the plant in the paper until dry, so that the driers may be changed without disarranging the specimen. Pressure should not be too great when plants are first put in the press; it may be increased when they become partially dry and there is no danger of crushing soft parts. After the day's collecting, if plants have been put in the press in the field, re-arrange any parts needing attention. Place a few ventilators between driers. Figure 1 Cage for rearing larvae. Figure 2 Mounted herbarium specimen with label. The first 12 to 24 hours will determine the quality of the pressed plant. Rapid drying preserves natural colors. Change the driers several times during the first day or two as they absorb moisture from the plants. Used driers may be dried in an oven or in the sun. To hasten drying, place the press where there is good circulation of air; leave it in the sun, or hang it over some source of heat. Mounting When the plant is thoroughly dry, mount it permanently on a botanical mounting sheet. If the plant is not longer than the sheet, mount it straight in the center; if it is longer, place the root toward the lower left corner and the top diagonally toward the upper right. Attach the plant to the mounting paper with narrow strips of mounting tape, using sufficient points of attachment to hold all parts of the specimen. Attach the label in the lower right corner (Fig. 2). A useful method of mounting is to coat a sheet of glass with Gelva solution, Formula 12 (p. 63), lay the specimen on and when it is well coated on the underside transfer it to mounting paper. Advantages of the method are neatness, strength, and the absence of mounting tape covering parts of the plant. Should it be necessary to remove the specimen, acetone will dissolve the adhesive. Preserving Green Colors In Liquid — Methods of preserving green colors in plants and foliage, and also the various colors of fruits are given in Formulae 20 to 27 (pp. 65 to 67). Formula 20, the boiling treatment to replace the natural greens with copper acetate, gives very good results when the specimens are stored permanently in liquid. Dry Preserving — Green colors may be preserved in plants by using Formula 28 or 29 (p. 67). The specimens may then be pressed and mounted. PLASTER MOLDS To reproduce plants and certain immature forms of insects in wax and plaster, it is necessary to prepare molds of the originals from which to make casts. The various plasters and methods of making plaster molds are discussed. Characteristics and Uses of Plaster Calcined gypsum, commonly called plaster of pans, is calcium sulphate- hemihydrate (2CaS04.H20). Variation in color of the gypsum rock determines the shade of the finished plaster, which may vary from white to gray. When water is added to calcined gypsum, it recrystallizes back to hydrated rock. This chemical reaction produces heat in the plaster as it sets. The heat of the mold indicates the time to remove it from the object being treated. Each type of plaster has a definite setting time, which may be controlled by adding accelerators or retarders to the water. The plaster generally used in Canadian museums is Hammer Brand Molding Plaster, formerly known as Hammer Brand FF, a Canadian brand. The company states that this plaster is processed to the same specifications as Red Top White No. 1 Molding Plaster of the United States Gypsum Company. The latter company processes a wide range of plasters suitable for different types of work which you may also buy from local dealers. Some of these plasters are described below: USG Red Top White No. 1 Molding Plaster — This is the standard form of plaster of pans and is the general-utility plaster used in many casting shops. The dependable uniformity in fineness of grinding and setting characteristics makes it suitable for molds, plaques, and general work. USG Red Top Superfine Gypsum Casting Plaster — This plaster has a finer grain than the above, giving smoother castings and excellent detail. It is ideal for modelling. USG White Art Plaster — This plaster was designed especially for cast- ings that are to be painted or that require a hardened, smooth-textured finish. Adding a surface-hardening ingredient minimizes paint absorption yet sacrifices none of the smooth-working qualities. To obtain the best and most uniform surfaces, dry these castings rapidly in circulating warm air. Trim and sand the castings before drying. USG No. 1 Casting Plaster — This plaster was developed recently for use when unusual surface density and hardness are desired. USG No. 1 Casting Plaster (Special) — This plaster excels in structural strength. A lower proportion of water is needed, giving greater strength and hardness. Castings are uniformly hard from core to surface. 8 USG Pattern Shop Hydrocal Plaster — This plaster is moderately low in setting expansion, giving accurate dimensions. The material sets gradually, has a long period of plasticity, does not get too hard, and can be carved. The normal mixture is 100 parts of plaster and 50 to 54 parts of water by weight. Setting time is 20 to 35 minutes. The setting expansion is 0.00125. Compressive strength dry is 3,200 pounds per square inch. USG Hydrocal A-ll — This is a high-strength gypsum cement with a low setting expansion and is suitable for making hard, tough models of uniform and stable dimensional accuracy. It sets rapidly after the setting actions begins. It is recommended for pouring or slurry casting technique. The normal mixture of this plaster is 100 parts of plaster and 40 to 42 parts of water by weight. The setting expansion is 0.0004. When set, its compressive strength is 4,500 pounds per square inch. Coecal Plaster — Coecal plaster, a Canadian dental plaster, is excellent for fine work. It takes only 1 part water to 3 parts plaster by weight and has a compressive strength of 7,000 pounds per square inch after setting for 22 minutes. To produce best results, the plaster should be sifted into the water, allowed to stand one minute, then spatulated to a creamy consistency. Vibrating the mix improves surface density and strength. It is usually possible to remove a mold after 20 minutes. Coecal plaster is more expensive than white molding plaster but it gives finer definition and allows wax casts to free perfectly from the mold using only hot water as a separator. Preparing Molds Plaster may be used for molds, casts, and carved models, or as one of the ingredients in mache when making bases. The proportion of water in the mix affects the hardness, strength, density, and porosity of the mold or cast. To obtain more uniformity in mold texture, precisely weigh or measure the ingredients and carefully record notes on the results obtained. Test for the correct amounts of plaster and water for particular plasters. For example, a good technique for hard molds is to use as little water as possible to form a mix that will pour. Whenever possible, it is best to use the normal setting time for each particular plaster. If in special work it becomes necessary to speed up or slow down the period of plasticity, or free-flowing state, add accelerators or retarders to the mix. A little glue added to the water will hold back the setting; common salt (sodium chloride) or potassium sulphate dissolved in the water will cause plaster to set more quickly. Accelerators in the plaster, however, will make molds more brittle. A mold is like a photographic negative. It is made to obtain a negative of an object from which to cast a positive, thus obtaining a detailed replica of the original form. Piece Molds — Those requiring more than one piece or section, must have enough pieces so that the cast can be freed; undercuts in a mold will lock the cast permanently. 54899-0—2 Fruit and Vegetable Molds — An apple, if not misshapen, usually releases freely from a 2-piece mold. A potato tuber, however, because of its uneven shape and deep eyes, requires three or more pieces. A material is needed for shaping around objects before making molds; the best material is water clay. A mixture of clay and sand that has gone through a brick or tile machine works well; it is not too sticky. Storing clay in a tight metal box with a little water in the bottom will keep it soft indefinitely. The following method of preparing a piece mold of a potato tuber in plaster will serve as an example. First if there is dust or soil on the potato skin, wash it thoroughly. Decide which side is to show in the exhibit, and where it will be best to join the several mold pieces and then: 1. Roll out a piece of clay to slightly more than half the thickness of the tuber. Lay the tuber on the clay, keeping uppermost the side to be featured in the exhibit. Mark on the clay around the tuber with a scalpel point and cut out the section, leaving a cavity that will fit the tuber. Line the clay cavity with thin, soft paper to keep it from sticking to the tuber. 2. Sink the tuber into the clay slightly more than half way. Model the clay smoothly against it, being careful not to cut the skin. 3. Cut a strip of thin card about two inches wide and long enough to reach around the tuber. Leave £ inch of clay all around between the card and the tuber. Apply vaseline to the inside of this band, pin the overlapping ends, and press it into the clay. This will act as a dam to contain the poured plaster that will form the first section of the mold (Fig. 3, left). Figure 3 Left, potato tuber in clay with dam. Right, first section with holes drilled for keys. Figure 4 Left, mold ready for second pour. Right, mold ready for third pour. Figure 5 Open mold showing pouring opening and holes for holding staple 4. Pour water (about 4 ounces for an average tuber) into a flat bowl and sift in twice the weight of plaster, more or less, depending on the type of plaster used. Let it stand for one minute, or until the water seeps throughout the plaster. Stir carefully to avoid trapping air bubbles until a smooth, creamy consistency is obtained. Use a brush and apply a thin coat of plaster over the exposed part of the tuber. This will avoid bubbles when pouring the balance of the plaster to a thickness of about I inch. Keep the plaster uniformly thick for each section of the mold. A thick area of plaster is likely to form a chalky surface on the mold. 5. After the plaster sets (when it becomes warm), remove the paper dam and the clay from the tuber which remains fast in the plaster. With a i-inch bit, make six hemispherical cavities in the plaster around the tuber to form keys for the next pour. This ensures perfect alignment with other pieces of the mold (Fig. 3, right). 6. Apply a thin film of vaseline to the edges of the mold section, working it well into the key holes. This acts as a separator for the next pour of plaster. Pin a strip of card, with vaseline on the inside, around the mold section, and insert a clay dam to expose half of the portion of tuber that was not in the first mold section (Fig. 4, left). 7. Paint plaster over the exposed area of tuber and pour the second section. When this section becomes warm, remove the paper and the clay dam. Drill holes for keys in the newly exposed edge and apply vaseline. Place a vaselined strip of paper around the mold making it ready for the third pour (Fig. 4, right). 8. Pour the third section and when the plaster is warm trim all sutures until clean lines appear at the joins. Release the mold from the tuber by wedging a knife blade into the joins until they separate; the pieces can then be lifted off. For a subject that will be fastened to the back of a display unit, carve a ^-inch hole through the underside of the mold for pouring the wax. On each side of this hole, bore a small hole to carry a wire staple for supporting the finished wax cast in the display case. Bind the two small sections of the mold together with fine wire to protect the edges of the join (Fig. 5). Two-piece Leaf Molds — For leaf molds, Coecal plaster lasts longer and gives better definition than white molding plaster. It takes only a small amount of the material to face the molds; ordinary white plaster may be used to build up the necessary bulk to give it strength and support. To be efficient, leaf molds should fit a hand press (Fig. 6, p. 13), used for casting and made of uniform size with tin forms. For molds 1\ by 5 inches by 2i inches high, cut two pieces of tin 151 inches long, one of them li inches wide and the other 2\ inches wide. Cut a wood block to the above mold measurements. With a mallet and a vise, bend each tin strip separately around the block, overlapping the ends at the join (Fig. 6). Select leaves with strong varietal characteristics, of normal size, and as fresh as possible. Leaves will stay fresh for a week or more if layered between wet paper in a tightly covered metal box and refrigerated at a few degrees above freezing. To make the molds, roll out a sheet of soft clay, cut it into pieces a little larger than the bases of the tin forms (do not use 11 54899-0— 2i clay that is too sticky) and press a form lightly on the clay to mark the area within which the leaf or leaves are to be placed. Apply vaseline to the insides of the tin form. When a single large leaf is used, place the petiole at one end of the mold. If you are making several small leaves in one mold, arrange them side by side with the petioles all one way. Model the clay under the leaves to support their natural shapes. Do not press the leaf or leaves into the clay but make sure all edges are in contact with it or plaster will run under when poured. If the leaf surfaces are soiled with clay, swab them clean with water. Place an elastic band or a cord around the narrower form, press it evenly into the clay. Paint Coecal plaster quickly over the leaf surface to exclude air bubbles. Pour on about I inch of Coecal plaster and fill the form with white plaster (small molds may be made entirely of Coecal). Just before the plaster begins to set, stroke off the top level with the tin form. Remove the plaster from the form after it has set. Remove the clay from the leaf very carefully so as not to loosen any leaf surface from the plaster. Should this happen, you must fasten the leaf to the plaster evenly or a thick spot will result on the cast; thin a little bookbinders' paste with water and slide it in between the leaf and the plaster with a fine brush. Press the thumb on the leaf back of the loosened area to avoid lifting more leaf surface. See that all the leaf edges are free from plaster that may have run under. Drill depressions for keys with a i-inch bit and apply vaseline to plaster surfaces with which the next plaster pour will come in contact. Apply vaseline to the insides of the 2i-inch-wide tin form and fit the form around the first piece of the mold surrounding the leaf, to contain the second pour of plaster.. Make sure the bottom of the tin form is even with the bottom of the plaster block and tie the form with wire or cord so that it fits tightly against it. Treat this second block the same as the first; after levelling the top, let stand until it sets. When the plaster becomes warm, remove the tin, trim the join if necessary, and tap the mold along the join so that it may be pried apart with a knife. Remove the leaf from the mold, wipe any bits of plaster from the face, and bind the two halves together with fine wire to dry (Fig. 7.). To make plaster molds for thin leaf casts, especially when the undersides will not show, use the following procedure. Remove the leaf from the mold piece after the first pour. Paint the plaster surface with a separator, Formula 32 (p. 68). Place the wide tin form in position and finish as described above. Cut a groove for the midrib wire in the under half of the mold. Molds of Larvae — Thoroughly remove chemicals from preserved larvae before molding. It is much better to use living larvae; any distortion in a preserved one will show in the mold. One of the difficulties is to keep the specimen from collapsing while making the mold. To obtain best results with large larvae, place the living larvae in cold water, bringing them slowly to a boil. Allow the water to cool with the larvae in it. Injecting absolute alcohol hypodermically through the anus will also harden the larvae. Very small larvae are best killed with ether. 12 Coecal dental plaster works well for molds of larvae. Cut a narrow strip of thin cardboard, apply vaseline to one side, overlap the ends and pin. Shape this dam so that it will surround the larvae evenly, and press the edge of the paper into a smooth piece of clay or plasticine. Pour plaster to fill the dam and level off the top, teasing any surface bubbles to the edge with a pin. With forceps, pick up a larva and, beginning at the caudal end, carefully lay it on its back in the plaster, sinking it evenly halfway. With the point of a pin, even the plaster where it touches the sides of the specimen. Allow the plaster to set, remove the paper dam, trim the plaster where necessary, and drill holes for keys. Paint a thin film of vaseline on the mold edge and pin another dam around the mold. It is now ready for the second pour. Cover the larva with a thin coat of plaster, blowing to rid the surface of bubbles, and then pour the rest of the plaster; alternatively the larva may be painted lightly with thin plaster and the pour made. Figure 6 Tin forms for leaf mold. Figure 7 Plaster mold of leaf. 13 When the second pour has set, separate the mold by wedging it apart with a knife. Scribe a fine channel with a scalpel point from the head to the edge of the mold to allow air to escape when injecting hot wax. Carve a funnel-shaped opening in the caudal end of the mold for injecting the wax. Preparing Plaster Slabs Use two pieces of plate glass and four spacer blocks of the required thickness. Mix plaster free of bubbles and to creamy consistency. Pour the plaster on one of the pieces of glass, to slightly more than the thickness desired. Make a high ridge down the middle so that the second piece of glass, when pressed on the plaster until it rests on the spacer blocks, will not trap air bubbles. No separator is needed. Allow the plaster to set fully before sliding the glass off. Cut and shape the slabs soon after freeing. When dry, they may be impregnated with thin Gelva solution, Formula 12 (p. 63). These slabs are useful for mounting small insects and showing life histories in exhibits. FLEXIBLE MOLDS Latex Molds: Description and Uses — Latex is made from the milky juice of rubber trees. For molds, natural latex is processed in such a manner that it will dry or vulcanize without heat. It is one of the best mediums for flexible molds, giving even better definition than plaster. Another advantage over plaster is the absence of troublesome flanges resulting from joins. It is not always possible to eliminate the flanges on wax casts made from plaster molds; with rubber molds the only openings are at the back where they do not show. Preparing the Latex Mold — The following is the procedure for preparing a latex mold of a potato tuber. Prepare a base on which to mount the potato tuber. The tuber needs to be mounted so that all the skin is exposed; the latex must not be touched with the fingers when it is drying. For a medium-sized tuber, cut a piece of galvanized iron sheeting 2 by 2f inches. Trim one end to conform with the contour of the underside of the tuber. Bend the opposite end of the sheet to make a right-angled flange i inch wide. Tack the sheet to a wooden block big enough to hold the tuber firmly when mounted on the tin. Cut two pieces of 1 8-gauge wire 2 inches long, and solder them vertically to the free end of the tin; keep them I inch apart and projecting I inch above the tin. Besides acting as a support for the tuber, these wires will leave two holes in the mold for receiving a wire staple when casting; it is used to fasten the cast in a display unit. To complete the base, cut a f-inch cork stopper in half lengthwise and fasten one half to the metal between the wires with Duco cement. Leave the small end up and trim to fit against the tuber. The cork forms 14 INJURIES TO SUGAR BEETS y WIRFWORM MEADOW K Figure 8 — Rubber mold in plaster jacket- showing treatment of undercuts in a sugar beet. Figure 9 — Exhibit of injuries to sugar beets. The lower model was cast hollow from the rubber mold illustrated in Fig. 8 (Displayed in Science Service Building, Ottawa.) a hole in the bottom of the mold through which to pour the casting medium (Fig. 10, left). Press the two projecting wires into the tuber until the metal sheet comes in contact with the skin, but without cutting it (Fig. 10, right). Use only a 1 per cent aqueous solution of ammonia for thinning; do not dilute latex unless it is necessary to pour or spray it. Between coats, brushes may be left in the ammonia solution or in a 10 per cent solution of soap flakes in water. After applying the coats of latex soak the brush in carbon tetrachloride and remove the latex by scraping should it become filled with partly dried rubber. No separator is needed when applying latex to a potato tuber; it is applied directly to the tuber. Six coats should be sufficient depending on the thickness of each. Apply the first coat evenly with a soft bristle brush or your finger over the entire surface, and about one inch down the tin and the cork. Drying time between coats depends on the thickness of the coat applied. To determine when to apply the next coat, press a hard object into the drying latex. If the impression disappears, the rubber is dry. Should the mark 15 remain, let the rubber dry longer. Apply additional coats similarly, making each coat even with your finger. Allow two or three days for final drying; pull out the wires and the tin, leaving the tuber on the mold. The next step is to prepare a plaster jacket, or "mother mold". This is a 3-piece jacket to support the latex mold for casting in it. Cover the mold with moist tissue paper and with the pouring hole at the top and the tuber still inside, embed the mold to half its depth in moist clay (Fig. 11, left). Cut a strip of thin cardboard wide enough to be level with the highest part of the mold when the cardboard is pressed into the clay. Apply vaseline to the inner side of the carboard, overlap the ends and pin to form a ring large enough to expose f inch of clay all around the mold. Push the cardboard into the clay. Cut a thin strip of clay and press it upright against the mold to act as a dam to retain the plaster when poured on half the surface of rubber above the clay base. It is now ready for the first pour (Fig. 11, right). Pour the plaster. When it sets, remove the paper containing-ring and the clay dam. Bore four hemispherical holes in the edge of this section of the plaster mold and apply vaseline as a separator. Place a strip of vaselined paper as before around the rubber mold (Fig. 12, left). Pour the plaster for the second section. When it has set, remove the strip of paper and peel off the clay. Drill holes as before in the edges of the two sections and apply vaseline as a separator (Fig. 12, right). Place a paper dam around the mold, exposing the rubber that has not yet been covered. Pour the plaster for the third section. When it sets, fi ppg ' \* .pp* Figure 1 0 First stage in preparing a rubber mold. Left, tin on wood base with wires a^^^ ■ "^ for mounting tuber. Right, tuber mounted ready for ^^^Hu ,fF Is- - jJm &f metal for casting temperature by dipping a piece of white paper into the melted metal momentarily. The paper should not burn, but turn yellow. Be careful that no free water comes in contact with the molten metal or it will explode. Pour the metal on one section of the impression, beginning carefully at the base and filing the cavity left by the plaster mold. Let the metal cool and remove it from the sand. Repeat the above opera- tion with the other section of the plaster mold and the two sections of metal will fit together as did the plaster mold (Fig. 17). Caution: Any disturbance of the impression of the plaster mold in the sand, before or during casting, will prevent the two pieces of the metal mold from fitting together. *\ Figure 18 — Metal mold is smoked to form a separater for the second pour. Preparing Impression of One Leaf Surface — Make one half of the metal mold exactly as described above, using the section of plaster mold of the desired surface. When it is cold, smoke the impression surface by holding it over a burning cotton cloth previously saturated with turpentine (Fig. 18) This will act as a separator when the pour for the next section is made. Cut a strip of asbestos paper twice as wide as the height of the metal section and bind it around the metal, leaving the smoked impression up. The asbestos will contain the molten metal until it cools. Be sure the metal section is cool and dry; then pour the metal, filling the asbestos ring. Never pour molten metal on a hot mold surface because it will stick. Casting in Sheet Plastic from Metal Molds Metal molds will not give the minute detail obtained by casting liquid plastic from plaster molds. However, where large numbers of leaves are needed, as in group exhibits, casting sheet plastic from metal molds is fast and useful. Casting Plastic Leaves from a Cold Metal Mold — Use molds that have been prepared by the method described under the above heading "Preparing Impression of One Leaf Surface". Cut a piece of sheet cellulose nitrate, .005 or .001 inch thick and slightly larger than the mold. Place it in a cold bath, Formula 46 (p. 70). When the sheet becomes rubbery, remove and wash it quickly in cold water. Quickly remove all water, using absorbent cotton. Complete removal of water is important to prevent defects in the cast. Place the sheet between the two halves of the mold, with the keys matching, and clamp the 21 mold in a vise until the plastic has set. Fifteen minutes or more are neces- sary. Trim the edges of the impression. If it is difficult to follow the edges on the clear cast, spray paint lightly on one side to bring out the detail. Casting Plastic Leaves from a Hot Metal Mold — Use iron tongs to hold the two halves of the mold together to keep the section in alignment and act as a handle to remove them from a boiling water bath. Tongs must be large enough to fit either end of the mold when the two sections are together. Solder the jaws of the tongs to the mold sections while the mold is closed and the keys registering perfectly. Cut a piece of sheet cellulose nitrate or acetate slightly larger than the mold, and place it on the leaf impression. Over this lay a piece of thin rubber (cut from a tire inner tube to the size of the plastic sheet) place the other half of the metal mold in position and clamp the mold shut. Place a ring tightly over the ends of the tong handles to hold the mold shut. Immerse the mold in boiling water until the plastic becomes soft. Then clamp the mold in a vice for ten seconds, change ends and clamp again. Glycerin in a double boiler with sand in the bottom sec- tion of the boiler will provide greater heat. Casting Plastic Leaves from a Metal Mold in a Hydraulic Press — A hydraulic press with controlled pressure from 10,000 to 30,000 pounds per square inch is needed. Leaf casts of good detail are rapidly produced. Tool out a channel for the midrib wire in the under section of the mold, place a wire in the groove and put the mold on a hot plate. Place tenite plastic in sheet or granular form over the impression in the mold over the wire, close the mold and position in the hydraulic press. While in the press, the plastic becomes softened to such a degree that it flows into all details. The wire for the petiole and midrib will be firmly embedded in plastic. CASTING IN WAX Casting in wax plays an important part in preparing exhibits. Fruit, vegetables, and the larvae of insects reproduced by this method are more lifelike than when cast in plastic or plaster. Foliage also may be successfully cast in wax; fewer molds are needed because it is possible to alter the shape of casts after they are made. Casting in wax involves making a mold or negative of the original object and pouring wax into it to obtain a positive. Fruits and Vegetables in Wax The proper separator to release wax casts from plaster molds should be chosen. No separating agent is needed for molds made with Coecal plaster heated in water but one should be used for white molding plaster. Use Formula 37 (p. 69). Melt pure, refined white beeswax in a double boiler. Turn the heat to low when the wax has melted. Tint the hot wax by thinning tube oil color with turpentine and stirring it into the wax. Formerly balsam was added to beeswax to temper the casts and make them less brittle; but, contrary to expectations, as the casts aged the balsam-treated wax became more brittle than untreated wax. Before casting, check the following points and little difficulty should arise: adjust the room temperature to 80° F, and the wax 22 temperature to 158° F; soak the plaster molds for at least 30 minutes in water held at 128° F. If the molds are made of white plaster, add the soap solution a few minutes before casting. Have a supply of special staples ready to anchor one in each cast. For a small cast such as of an apple or a potato, make the staple from a 3-inch piece of 18-gauge wire (bend the ends at right angles to the center part). The crosspiece of the staple should be i inch long. Apply shellac to the crosspiece and bind cotton around it. Casting Solid^To cast, remove the mold from the hot water and swab the inside with cheesecloth dipped in hot water and wrung out. Insert the prongs of the staple from the inside of the mold through the holes pre- pared to receive them, leaving the cotton-wound crosspiece projecting into the mold \ inch or more to make sure that the staple will be well anchored in the cast. Assemble the mold hot and, with a ladle large enough to fill it in one pour, fill the mold. It is usually necessary to add a little wax as it cools. Casting Hollow — The procedure for casting hollow is the same as for making a solid cast up to the time the mold is filled with wax; wait a moment and then pour out nearly half. A good way to judge the quantity removed is to pour it back into the ladle. Stop the hole with a cotton plug held by the thumb and rotate the mold in every direction; the wax will cool in an even layer over the entire mold. Open the hole from time to time during the early part of the rotation to allow heated air to escape. Continue rotating for a short time after no liquid is heard when the mold is shaken. Release solid or hollow casts when the wax has set but is still rather warm. Remove the wire holding the mold pieces and pry the pieces apart slightly with a knife to loosen them from the cast. If a small cast sticks in a piece of the mold, hold the cast in the hand between thumb and fingers and just above the palm. Tap the mold gently with a wooden mallet or a small hammer; this will usually release the cast. A slight flange is left on the cast where the mold pieces join. Remove this while the wax is still warm, with a spatula, modelling tool or other instrument. If any disfigure- ment remains, dip your finger in carbon tetrachloride and rub it along the tooled area. Do not remove any wax from either side of the flange. Rest the cooling wax cast on two wooden blocks, with the staple between, until the wax is cold. To make a hollow cast that will be viewed from all sides, fill one half of the mold with wax and replace the other half. Rotate until the wax sets. Even with this method it is necessary to make a small hole in the mold which you can open occasionally to release hot, expanded air; otherwise it will force its way out between the joints and ruin the cast. Causes of Difficulty in Casting in Wax • If the cast sticks to the mold, the wax or the mold is too hot or the mold has not been thoroughly soaked. • If there are cracks in the cast, a draft chilled the mold on one side while the wax was cooling. • Pits in the surface of the cast are caused by droplets of water left on the mold. • If there are waves on the cast, the mold or the wax has been too cool, or the wax has not been poured fast enough. 23 04 H O Q CO CO O H O o o. cvj * I o> o c o *l * a I:? C (A O 1° It. 2 o-2'S 4. °- »+. i- <*. Q) O . WO .O — U - o c ir 3 SJ :?3 CO W CO CO s O O 0) * I 0 I II = ° •/> "^ o 73 w 3 i. DO o .o o 3. if »- £ • 01 a u "» 5 o.E o .2 *. a Is UI m :* 3 24 X o c "i •" o = * oO *- o .. E £ o .= *--o l/l 1- 0» 0) Q.«/» E 2 O c (A 0) V4- *U o V> •t E JS — J=-o X CJ Ul >s - "5 o ^ k> 3 Cft 2.E ji o w to o v on c o.SJ £ °^ ,.2 — a o c »*. ± O C 3 *■— o E «" I - £ « .. o "Z *• o — Ov -Q ~ o £.2 o «. 1- 0 *- *- c c o ^ 1) o 4- c o L. o o X on o o -1 XI X) >- u Ol «J o ■*- o o ■4- £ o X 4- 00 ex 3 01 32 JO *i D . 4- v. ■»- a» o c E ? -•- TjJ 0 JH 3 CO j: u 1- a 0) to l/> 01 V -c u •*- c - o a o m 5 o k. 3 Ol 33 Casting in Plastic from Rubber Molds — Walters' method of casting leaves with liquid cellulose nitrate from rubber molds has been used with good results. Latex takes finer details than plaster and eliminates the extra work of removing plaster from the cast; it can be removed without a separator. The method has its limitations, however. It is more applicable to small leaves, because these may be fastened more securely than large ones to bases to counteract curling caused as the plastic dries and shrinks. To avoid flat places on the mold, pour a "pancake" of plaster on glass. Lay the leaves in rows on the plaster but be careful not to trap air under the leaves. Make sure that all edges are in contact with the plaster. Let the plaster harden. Make the rubber mold in one sheet; no separator is needed. Paint the coats of latex directly on the leaves and surrounding plaster, making the coats uniformly thick over the entire mold. Let each coat dry before applying the next one. While the second last coat is still tacky, sprinkle a layer of glass fibre or absorbent cotton on the mold for reinforce- ment; the final coat over this acts as a bond. Allow at least three days for drying before releasing the mold. Before casting, tack the mold all around the edge on a thick plywood board; this avoids curling while the plastic cast is drying. Casting proceeds in the same manner as with plaster molds (Fig. 32.). Cold Press Casting of Leaves and Petals This method has been practised in several museums. Clear cellulose acetate sheet was used as the casting medium by some, but for large leaves or petals cellulose nitrate sheet makes stronger casts. Preparing the Molds — Hang the leaves or petals individually in a row by running pins through the bases, exposing the undersides. Mix plaster, and paint these surfaces with successive coats until the plaster holds the leaves firmly in position. When the plaster has set, remove the leaves or petals. Roll out a sheet of soft water clay. Lay the plaster mold, from which the leaf or petal has been removed, face up on the clay. Model around the mold to form a smooth edge on the clay. Follow this procedure through the series. Place a dam or form around each mold to contain the plaster that will be poured. Apply a separator, Formula 32 (p. 68), to the plaster. Paint thin plaster over the mold to exclude air bubbles and pour additional plaster to a depth of 1 inch. When the plaster has set, separate the pieces. Casting — Prepare a lump of plasticine a little larger than the mold. Press the face of the last section of the mold poured into the plasticine until a good impression is formed. Cut a piece of cellulose nitrate sheet, .005 or .010 inch thick, slightly larger than the mold. Place it in a cold bath, Formula 46 (p. 70). When the sheet is limp and rubbery, dip it in cold water and quickly remove all water with absorbent cotton. This is important. Any water left on the plastic will cause defects on the cast. Place the sheet of plastic over the impression in the plasticine and press 34 Figure 32 Rubber mold for casting leaves in liquid plastic. Figure 33 Cold-press casting in plastic. Plaster mold, plasticine, and cast leaf. the mold firmly into it. Lay a weight on the mold and allow it to set; time needed is usually about fifteen minutes. Trim the waste edges. If the cast is a petal, it is ready for coloring. With leaf casts, fasten a plastic- coated wire on the back of the midrib base with Duco cement to form the petiole (Fig. 33.). To color this type of cast, use artists' tube oil colors thinned with spirits of turpentine. If no gloss is desired, add 25 to 50 per cent carbon tetrachloride to the turpentine. With casts having sharp details, the pattern may be worked out with an airbrush by spraying across the cast surface, just striking the high parts. Iridescence may be attained by laying one color over another. Do not use vermilion or the cadmiums if translucency is desired. A Plastic Material for Cold Molding Methyl methacrylate comes in liquid, granular, and sheet form. The addition of a plasticizer such as dibutyl phthalate forms a paste that can be molded. The resulting medium has a white, marblelike translucence. This material is useful in building up thick midribs in plastic leaf casts. 35 Casting Foliage for Miniature Groups Rowell (34) used type metal or stereotype plates for mold material. He sketched the leaf pattern on the metal face and worked out details with engraving tools. A separator was applied and the cast made in liquid cellulose nitrate, which was released from the mold by soaking. The Harvard College forest models in the Petersham, Mass., Forestry Museum made by Theodore Pitman are excellent dioramas. Copper wire was twisted together to form branches of trees, and the long ends were bound together by wrapping wire around them to form the trunks. Needles and leaves were made of sheet copper by photo-engraving process, soldered to wires, and twisted into the branches. The branches were dipped in molten solder to stiffen, and colored by hand painting and spraying. Embedding in Plastic This method may be used for displaying preserved biological materials. The embedding medium is a syrup of polyester resin. The catalyst may be one of several chemicals; Ward's catalyst for their monomer (Bio-plastic) is tertiary-butyl-hydroperoxide. Adding the catalyst to the monomer causes it to jell, bringing about the process of polymerization. It is later cured by heat to give a hard, clearer-than-glass plastic in which the specimens are embedded. Much has been published on this method and the techniques involved need not be discussed here. Ward's (5 1 ) gives the results of a wide range of experiments, both of embedding in plastic and of casting from flexible molds. Special treatments for various subjects are described. Apparently insects were the most difficult. Fessenden (8) dealt very thoroughly with the preserving of natural color in plant specimens mounted in plastic blocks. The Castolite Company (6) in their instruction manual have explained the simple method of embedding in plastic without heat and the professional method with heat. The publication also describes the casting of solid subjects in plastic from flexible molds. Raizenne (30) adapted the method to display deciduous leaves. PAINTING AND ASSEMBLING Painting Correct color and texture are important features of plant and insect exhibits. The form and detail are obtained by careful casting, but faulty finishing may ruin an exhibit. Use good artists' oil paints in the painting process. Painting 3 -dimensional objects is very different from painting on a flat surface. Shadows in pictures are fixed, but insect and plant exhibits must be colored so that the lights used to display them produce the highlights and shadows naturally. All colored objects, when placed in display cases, tend to appear darker; it is important to use tones that are not too dark. 36 Fresh insect or plant material from which molds are made is seldom available by the time casts are ready for painting. It is imperative, therefore, to make good color sketches from the subjects while they are still in a fresh, natural condition. Make the sketches in oil paints if oils are to be used to color the exhibit. When it is feasible to use the airbrush it is possible to obtain more lifelike results. This is especially true with thin leaves; transmitted light brightens them and shows brush marks if they are hand-painted. With all subjects, it is well to use paint sparingly. An object that looks painted never duplicates the original. Beeswax dissolved in benzene or xylol and lightly tinted and sprayed on painted wax casts of fruit will give them greater depth of color. Adding a little Damar varnish to the solvent produces a glossy effect on the cast. Many special color textures may be obtained. For example you can duplicate the flaky effect on the skin of new potato tubers by painting a coat of liquid plastic on a rubber glove. When it dries, stretch the glove and the film of plastic will crack and curl. Apply the flakes to the surface of the potato while the paint is still tacky. Assembling Plant Parts Before molds can be made of a plant, it must be taken apart to prepare molds of the individual parts. The drawings and color sketches made at this time determine the accuracy of the final assembly. In plant sketches give the general outline of the plants, positions of leaves, and distances between them. A convenient method of identifying positions of leaves on a plant sketch is to number them clockwise or counterclockwise around the stem. A Potato Plant — A potato plant will serve as an example. Assume that all the wax leaflets are cast, shaped, colored, and lying in position on the original sketches of the compound leaves. The terminal leaflets will have wires long and strong enough to support the lateral leaflets. File flat areas on the rachis wires for attaching the wires of the lateral leaflets; flatten all wire ends of the leaflets parallel with the leaf blades and bend to fit the central wire for soldering. Solder all leaflet wires to the rachis wire, beginning with the first pair below the topmost leaflet. Place the rachis wire in a vise. See that the flat areas on the wires make even contact then apply soldering flux, Formula 19 (p. 65), to the wires and solder quickly. The heated areas of the wires must be cooled immediately or the heat will travel through the wire and melt the wax. To cool quickly apply wet swabs of cotton or pour a spoonful of cold water over each join. Tint beeswax pale green and build up the rachis by applying successive layers of hot wax with a spatula. Model the rachis making smooth con- nections with all petioles. Model the characteristic grooves on the rachis surface using a specially shaped scribing tool. To prepare this tool, flatten the end of a wire and file it to make a template to conform with the pattern on the original stalk. Scribe the rachis while the wax is warm but not too soft. 37 Figure 34 Compound leaf. Upper, potato leaflets soldered to central wire. Center, leaf stalk modelled in wax. Lower, stem and midribs masked for spraying leaf blades. Spray the entire compound leaf with pale, greenish yellow as a ground color. When the paint is dry, cut masking tape to fit all midribs, petioles, and the rachis. Fit these in their exact positions before further spraying (Fig. 34). Spray all the leaf surfaces with the blade color, leaving the undersides slightly lighter. It is good practice to test the density and evenness of the spray by holding the leaf between the eye and a light. To blend colors, remove all masks and spray the entire leaf very lightly with the last color used. A pale green tint of paint sprayed lightly will represent the bloom usually seen on plants. Dip a bristle brush in white cotton flocks and stipple the freshly painted surfaces to duplicate pubescence. Cut a piece of 12-gauge galvanized wire for the plant stalk and taper one end for the top. File flat areas for the compound leaf attachments. Locate the points of attachment from the drawing prepared from the original plant stalk. Apply shellac to the wire and wind it tighly with cord, keeping it away from the flat areas; this will act as a bond for the wax modelling. It is easier to model wax over this wire before attaching the compound leaves. In order not to lose the points of attachment, solder a section of a 38 common pin at the upper edge of each flattened area. Model hot wax over the wire to the size of the original stalk. Cut a section of wax away just below each pin marker. Beginning at the top, solder each compound leaf in place and model over the joins with tinted wax. Prepare the winged flanges of the stalk by using Japanese paper dipped in pale green wax. Cut narrow strips from this, fold them sharply lengthwise, and cut narrower strips from the folded edge. Cut grooves in the wax with a scalpel point and insert the strips, building up against them to restore the original shape. A final light spray finishes the coloring. Insect injuries and other fine hand painting should be done under a lens. When the painting appears satisfactory through the lens, there should be very few brush marks noticeable with the naked eve. Branches of Trees — Green sections of tree trunks or heavy tree branches usually crack badly near the cut ends while drying. This may be overcome by coating the ends with melted beeswax and making a fresh cut above the wax when the wood has dried. When assembling exhibits of tree branches in permanent cases, it is often difficult to attach small ones to the back of the display case. Make special drills by grinding sewing needles of various sizes to drill points. Use a speed drill with a foot control so that both hands will be free; fasten the drill head to a small bench vise. Hold the branch against the drill point to drill the holes. Arrange the branch in the case and push a pin through each hole to mark for drilling in the back of the case. Drill the holes with the same size of drill as was used for the branch. Push the pins in the branch through the Figure 35 Exhibit of insects on a hill of potatoes. Prepared in wax by casting and modelling. (Displayed in Ento- mology Laboratory, Chatham, Ont.) 39 holes in the back and clinch them into the wood. The pin heads in the branches can be hidden by paint or covered with bits of bark. Fruit and Vegetables — Casts of small fruits and vegetables are temporarily mounted on wooden blocks for coloring and storing. Two holes are bored in the blocks for inserting the staple prongs. To assemble the finished casts in a display unit, make a template of thin cardboard to fit inside the unit. Mark the label positions on the template and place the models on blocks in the arrangement they are to have in the finished exhibit. Mark around each block on the template and for each item use the following procedure. Remove a cast from a block, cut a paper the size of the top of the block, and mark the positions of the two holes. Lay this paper within the outline of the block on the template and mark for the two holes. When ready to attach the models, first fasten the labels, from measurements on the template, with bookbinders' paste. Then lay the template in place and punch through the pairs of hole marks into the case back to mark for drilling. Drill all holes with a drill the size of the staple wire. In the back of the case cut a slot between each pair of holes to receive the wires when clinched to hold the casts. For soil profiles made over wood and mache forms, fasten with two screws through the back of the case. Mount photographs in exhibits by applying bookbinders' paste over all the back surfaces of the prints to keep air from getting under the prints and buckling them during weather changes. A photographer's rubber roller is a useful tool for obtaining an even contact of photographs and labels in the cases. PRESERVING CONIFEROUS FOLIAGE Fortunately, it is not necessary to reproduce artificially the foliage of coniferous evergreens. A recent technique by Raizenne (29) retains the needles on the twigs almost indefinitely. After treatment, painting will restore the natural color. Soft needles of spruce or balsam in the new spring growth do not process; after curing they will shrink and curl as they dry. To overcome this disadvantage, duplicate the new growth artificially or use older needles of the proper size and depend on coloring to simulate the new growth. Ethylene Glycol-Gelatin Method — Select branches of suitable size and shape and process them as soon as possible after cutting. Pines, balsams, and other evergreens with a high pitch content should have the surface pitch removed before treatment. Thoroughly wash all frass, webs, and dirt from evergreens with severe insect injury before they go into the glycol bath. Any substances left on the needles when the branch is dipped in gelatin will be sealed in, causing roughness over the foliage. Submerge the branches for two weeks in the ethylene glycol solution, Formula 30 (p. 68); remove excess preserva- tive by shaking gently. Dip several times in the gelatin solution, Formula 3 1 (p. 68), while it is still warm. Shake the foliage well after each dipping to remove excess film between needles. 40 EUROPEAN SPRUCE SAWFLY Gilpiniae hercyniac (Htg.) HEALTHY LARVAE FEEDING ON NEEDLES Of SPRUCE COCOONS SPUN UP IN GROUND FEMALES LARVAE DEAD AND DYING FROM VIRUS DISEASE ADULTS FEMALE LAYS ABOUT 45 EGGS LARVA INSIDE - i ADULT INSIDE - ADULT EMERGED «» 1 EATEN BY SHREW VERY RARE AND DOES NOT FUNCTION : FEMALE PRODUCES YOUNG FEMALES WITHOUT MATING LAYING EGG IN SLIT MADE IN NEEDLE WITH SAW-LIKE OVIPOSITOR Figure 36 — Exhibit of the European spruce sawfly. Preserved foliage, colored. (Displayed in Forest Biology Laboratory, Fredericton, New Brunswick.) With evergreens having closely set needles, it is necessary to separate the needles after each dipping so that they will not stick together. When two needles dry in contact, you will tear the gelatin coat when separating them. Allow a few days to dry and then color the needles with oil paints to match the prepared color chart. Use thin oil paint and with a fine sable brush paint each needle individually. Starting at the base of the needle make each stroke continuous to the tip. The bloom effect may be obtained by spraying with a pale paint mix. If the needles are not firmly attached after processing, it is the result of one of two things: either the branches were not treated in the glycol bath soon enough after cutting or not enough gelatin was deposited over the needles. 41 54899-0—4 Mr. Emil Sella, Natural History Museum, Chicago, processes his ever- greens with glycerine and restores the color with an airbrush, removing color on the branches with a brush and turpentine. This method of color- ing may be used for group work, however hand painting of small sections to be viewed closely will produce finer results. Glycerin-Shellac Method — Other methods of preserving evergreens are avail- able. Mr. D. M. Blakely (in litt.), Cranbrook Museum, Bloomfield Hills, Mich., using an acetone-alcohol and glycerin-formaldehyde method, pro- cessed a black spruce tree in a habitat group which was very successfully mounted in a museum, but was too fragile to ship. Processing Larch Branches — The glycol method does not work well on larch. The leaves are held firmly in the fascicles, but they curl and shrink. Select branches with mature leaves. Place them in glycol solution and agitate the individual branches until the leaves separate in the fascicles. Leave them in a natural position for two and a half weeks. Remove the branches from the preservative, shake each well, and dip in warm gelatin solution while still wet. Remove the excess gelatin by striking each branch gently on paper. Dip one branch at a time and shape it before the gelatin dries and seals the needles together. Use an air hose with a pressure of about 25 pounds with a circular motion on each fascicle until the needles stand free from one another. Separate needles that stick together. When the gelatin solution has become cooler and the branch partly dry, dip it again taking care not to melt the first coat. Repeat the air nose treatment and pin in position on a sheet of soft plywood, cork, or corrugated paper. Separate any needles in contact and allow the whole to dry thoroughly. After the branches have dried, spray them with gelatin twice more to strengthen the fascicles. More natural foliage may be made artificially from thin sheets of plastic cast in color. Cut needles individually, assemble them in fascicles and fasten to the branches with "Duco" cement. BUILDING ENLARGED MODELS For exhibiting certain insects, it may be necessary to prepare enlarged models. These appear as seen through a microscope with all external parts highly magnified. This is the only graphic method for exhibiting small insects other than using enlarged drawings or photographs. Make models from suitable materials such as plastic, plaster, wax, wood, or metal; in each case the medium or media chosen must be suitable to portray the several parts of the insect. The detailed procedure that follows is an example; it was used to prepare an exhibit of the wheat stem sawfly for the Empire Exhibition in London, England, in 1949 (Fig. 37.). An Exhibit of Wheat Stem Sawfly — To avoid making molds and casts for the enlarged stems of wheat, a medium of the correct size, I inch in diameter, was found in tubes and solid rods of cellulose butyrate. On these it was 42 HOW THE WHEAT STEM SAWFLY HARMS THE CROP HOW SCIENCE RESCUED THE FARMER'S CROPS Figure 37 Exhibit of the wheat stem sawfly. Models enlarged six times. Made of cellulose butyrate. (Displayed at Permanent Canadian Exhibition, London, Eng. for some time after 1949.) possible to model furrows imitating those on a wheat stem. These furrows were longitudinal in groups of two to four fine ones alternating with a deeper one; a short length of the material was used for a trial modelling of the surface texture. The Stems — You use six tubes and two solid rods of cellulose butyrate for modelling the six wheat stems that appear in the exhibit. Cut the tubes and rods to the desired lengths and leave \ inch to spare on each one for later trimming. This practice will also allow cutting of the striae — which is described later — to reach the ends. To form a node, the swollen joint on a stem, cut two sections from a spare piece of tubing; make one piece H inches long and the other 1£ inches long. Split each down the side and place them in hot water to soften. Apply "Duco" cement around the tube where the node is to appear and place the longer section from the hot water around the tube on the "Duco" cement. Fill in the gap caused by spreading of the section with a piece of the same material. Repeat with the shorter section, placing it over the first. When the adhesive has hardened, grind to the shape of the original node. Model the striae, the fine longitudinal grooves on the stem, directly on the plastic stems. A standard 10-inch file has striae properly spaced to represent the pattern on a wheat stem enlarged six times. To make a holder, or sled, for the cutting tool and a base to guide it, proceed as follows: Nail two boards 2 by 20 by T% inches to a base board 4| by 20 by f inches, leaving a slot in the center into which the butyrate tube fits snugly. Gouge a hollow in the base where the node will rest. To make the sled, which will hold the cutting tool, fasten a piece of wood 3 by 5 i by f inches with glue and screws on the edges of two blocks, each If by 3 by I inches. Fasten a 10-inch file horizontally on the end of the sled with four small screws. The bottom edge of the file should lie about i inch from the bottom of the sled runners which rest on the outside edges of the baseboard. 43 54899-0—4* Screw a stopper on the end of the base to hold the plastic tube from slipping endwise. Fasten the base on the bench, pointing away from you. With the plastic tube in the slot, grasp the sled with the file on the side next to you, and tipped so that the corner rests against the plastic. With a firm, continuous stroke, pull the file the full length of the stem. Turn the plastic tube after each stroke so that all the spaces between the striae are equal. Figure 38 — Tool for scribing striae on enlarged plastic models of wheat stems. Now cut the deeper, longitudinal furrows, mentioned above. With a fine-pointed scalpel, cut a groove in the plastic tube where it rests in the slot; use the wood against which it rests for a guide. Turn the tube after each groove is cut and continue around the tube. Make these grooves about i inch apart, but vary the spacing slightly. With the striae now completed, cut the stem ends to shape and slice out sections of the stem to expose the interior as illustrated in Fig. 37. Using a ^-inch steel bit in a speed drill, model the texture of exposed interiors and the cut ends. With the bit held vertically, draw it over the surface of the area being shaped. If the drill is held lightly, its slight bounding motion will form small pits in the plastic which will then resemble the natural texture. Heat the modelled stems in hot water and bend them to the desired shapes; holding them in correct position, dip them in cold water until they set. The Leaves — Cut a leaf section from a wheat stem, and separate the blade from the sheath. Flatten them under a sheet of glass. Draw enlarged patterns from them (Fig. 39A). Fasten a sheet of cellulose acetate 0.002 inch thick over the patterns and, with a sharp-pointed tool, scribe the outlines and all striae appearing on the pattern. Cut out the two sections and sand- paper both sides of each lengthwise to make the proper texture. Bind the sections in position temporarily around a piece of f-inch rod of butyrate. Place the leaf and rod in hot water until they are well heated and then dip them in cold water while they are still bound on the rod. Remove them, lap the edges slightly, and fasten the sheath with ligule to the blade with 44 "Duco" cement. Joining these two pieces, one with a straight edge and the other with a concave edge, causes the leaf blade to stand out from the stem as in nature (Fig. 39B.). The Stubble — The surfaces of pieces of tubing used to represent stubble have no striae; they are just sandpapered lengthwise to remove gloss. Cut triangular sections out of the bases of tubes used for stubble so that the remaining points, when pressed together, will form rounded ends. Heat the bases in hot water and bend the points until all the cut edges join. Fill any gaps with butyrate chips softened in a flame. To form roots for the stubbles, rip pieces from a plastic rod to the approximate sizes of the roots required. Dip them in acetone and scrape to shape. Attach the roots to the stems by boring holes to fit the root bases and insert the roots. Build up around the root bases with thick liquid butyrate. Model all cut edges and exposed interiors of the stubbles as described for stems. Sheaths on dry stubble are usually badly worn by wind; this condition must be simulated in the enlarged sheaths. Make them from a 0.005-inch sheet of cellulose nitrate that has been scribed for striae and painted with brownish yellow acetone. This colors and withers them in one operation. Cut out the sheaths then shape and fit them as previously described. Coloring the Stems, Leaves, and Stubble — Coloring of the models is very important. This is best accomplished by spraying, using masks to cover parts not to be colored in the first spraying. Cut pieces of masking tape to fit all cross sections of stem ends, openings made to expose interiors, and the light areas at the bases of the leaves. This way you expose only the exteriors of the stems and the portions of the leaves and sheaths to be colored green. For coloring enlarged stems and leaves in plastic when color has not been incorporated into the medium, best results are obtained by applying the color with an airbrush. Lay one tint over another to give a more lifelike appearance. Mix three tints from tube oil colors — a pale green for the first coat, the full blade or stem color next, and last a bluish white — the last represents the bloom, a light powdery-appearing effect found on many plants. To thin the paint, use Formula 43 (p. 70). Hold the airbrush well away from the object and at right angles to it. Apply the various coats one after the other without waiting for the previous coat to dry. When the paint is dry, remove all masks and spray the exposed interiors lightly with sap green and white tint. Spray the stubble white, and then yellowish brown, made by properly blending yellow ochre, zinc white, and raw umber. The bases of all stems and stubble that will be covered by sheaths may be left uncolored. The Female Adult Sawfly — This model was made mainly by modelling with liquid plastic over a plaster core and using wire reinforcements for the antennae and legs. The construction proceeded as follows: Model the head, thorax, and abdomen in one piece from white beeswax, working directly from the microscope or an enlarged drawing. Make a 2-piece plaster mold from this model and from the mold cast the body of the enlarged model in plaster. Run a 16-gauge wire through the center of the cast from the head through the ovipositor. Leave a length of wire with 45 which to mount the model. When the plaster cast is dry, paint it with black cellulose nitrate until no white plaster shows. Paint the color pattern on the black plastic with yellow liquid plastic. Cut monel wires for antennae from 0.018-inch wire and for legs from 0.022-inch wire. Coat the wires with tinted liquid cellulose nitrate thick enough to model antennae and legs. For the spines near the joints of the legs, use sections of entomological pins. Drill holes in the head and thorax to receive the antennae and legs. Mount the natural wings on a microscopic slide and project them to the correct magnification on a card. Trace the wing margins and the venation pattern. Fasten a piece of cellulose nitrate, 0.005-inch thick, over the wing outlines and trace them with a sharp-pointed tool. Cut out the four wings and paint the grooved sides with black India ink. Before the ink dries, wipe the plastic, leaving ink in the grooves only. Mix oil paint to match the wing tint, add a drop of essence of pearl to give slight iridescence, and spray lightly on both sides of the wings. Attach the wings to the thorax with "Duco" cement. Mount the model on the stem by boring a hole through it to carry the wire protruding from the ovipositor. Adjust the antennae and legs and fasten them in the holes prepared in advance (Fig. 40.). The Larva — Model the larva in wax from microscopic examination or enlarged drawings. From the wax model make a 2-piece plaster mold, and from the mold cast in wax the several larvae needed. Secure proper-sized spines from cactus leaves or a similar source and insert them in the casts for setae. Assembling the Parts — To attach a leaf to the stem, apply "Duco" cement to the inside of the sheath and bind it in place on the stem with a cord. Let it dry thoroughly. To fasten a wax cast of a larva in the stem or stubble, embed entomological pin points in the stem at the point of attachment, place a piece of cotton saturated with "Duco" cement between the pins, and press the larva firmly into position. Distribute frass made of tinted plastic sawdust around the larvae. For the plug in the end of the stubble section, showing the position of the overwintering larva, use cork covered with brown-tinted plastic sawdust. Figure 39 Left, enlarged pattern of section of wheat leaf. Figure 40 Model of the wheat stem sawfly in plastic. 46 To fasten the exhibit in the case, make two rectangular staples for each stem, the middle section of the staple is 1 inch across. Cut two grooves in the underside of the stem deep enough to anchor the staples, with the middle cross section in the holes. Fill the grooves over the staples with liquid cellu- lose butyrate. The two prongs of each staple go through the back of the case. The base of this exhibit measured 20 by 27 inches. Animated Models Construction of enlarged models opens a large field for mechanically minded operators, especially for making animated models. Carmel (5), of the Cranbrook Museum, Bloomfield Hills, Mich., constructed three animated fireflies, enlarged 17 times. By means of a synchronous motor and a series of cams operating microswitches, the abdomens of the fireflies were illumi- nated in proper sequence of timing. Balsa wood, plastic, and metal were used. He also made a model of a blue-bottle fly, enlarged 46 times, that showed the halteres in motion while the case lights were on. Uses of Glass in Model Making — Von Fuehrer (47) used blown glass to make insect models; legs and antennae were strung in segments on wire. Glass takes paint and plastic coating satisfactorily. AIRBRUSH TECHNIQUE An airbrush is almost indispensable for preparing exhibits. Wax and celluloid leaves painted with a standard brush appear uneven and blotched when viewed by transmitted light. With the airbrush, it is possible to apply successive light timings to ensure even coverage and proper color tones. It is ideal for applying the ground color on wax fruit but not altogether satisfactory for spraying preserved evergreen foliage because it is not feasible to mask the branches. Painting the branches the same as the needles with the airbrush, will result in some loss of natural tints from the bark when the paint is removed. Types and Performances — Wold, Paasche, and Thayer and Chandler are three popular makers of airbrushes (Fig. 62, p. 74); these brushes have been used in museums, some preferring one and some another. With the Paasche ABG and the Wold BBF brushes, you can use thicker oil paint which is an advantage when producing special finishes with talc, soil, or flocks on freshly painted surfaces. Small airbrushes made to use water colors may prove difficult to use when applying oil paints. Low surface tension in oil paint allows the paint to run through the paint slot and hang on the tip of the cup. If the paint is too thin it will be blown off and spatters the work; if it is too thick, the paint slot is not kept full and the spray stops. Only experience will show the operator how to mix oil paint suitably for use in airbrushes. If you use oil paints, buy the best artists' paints and thin when needed with Formula 42 or 43 (p. 70). Strain the oil paint used in airbrushes but use paint as it comes if mixed thick for special uses, as mentioned above; spatulate it on a palette and when the paint runs sluggishly from the spatula, it is in proper condition for spraying. 47 Before attaching the airbrush to the hose, blow the hose out well to remove any dirt or moisture. Check the needle point to see that it is in perfect condition. Never leave an airbrush for more than a few minutes before cleaning. Flush it with turpentine or carbon tetrachloride until all paint residue is removed. Air Supply — A constant air supply adjustable from 25 to 30 pounds pressure is desirable. An electric compressor unit with capacity in this range will give the most satisfactory results. Open the drainage valve periodically to blow out accumulated water and dirt from the tank. Check the oil level in the crankcase and oil the motor bearings at regular intervals. When a compressor unit is not available, a cylinder of compressed, liquid, carbonic acid gas may be used. With most cylinders, it is necessary to have a reducing valve to control the working pressure. When reducing pressure, it is important to reduce the pressure at the cylinder first. For detailed instructions in air- brush technique, consult Kadel (15) or Tobias (40). Figure 41 — Gauge for liquid carbonic gas outfit. BUILDING INSECT DIORAMAS More and more museums are using dioramas to portray subjects in their natural habitats. These exhibits produce the illusion of a view through a picture window. Up to now, dioramic exhibits have mainly portrayed large species of animal life; little has been done to adapt the method to exhibit insects. An insect group must necessarily be small so that the habitat does not dominate the exhibit. By carefully choosing subjects successful groups may be prepared. The method would be useful for portraying severe out- breaks by insects such the armyworm and the Japanese beetle. General Principles — In such a group the foreground, consisting of actual insects or reproductions of them and the injury they do to plants, blends with a curved, painted background suggesting the area of infestation. To be successful the groups must depend entirely upon illumination from within the case; it should be stronger than room light to prevent reflections. It is impossible to give any illusion of distance in too small a case. No attempt should be made to build an insect diorama with a depth of less than 42 inches. For correct perspective the ratio of depth to width should be 6 to 10. Place the horizon line at eye level at a height of about five feet. The glass front should be smaller than the background to hide the ends and top of the painting and also to avoid distortion of the background. The glass should slope slightly outward at the top to eliminate reflections. 48 Base and Foreground — The base should slope slightly upward toward the back so that it will more readily blend into the background. Prepare soil surfaces as follows. Build a wooden form, cover it with galvanized wire screen, and model the soil contours with mache (any one of Formulae 38 to 41, pp. 69 to 70). Sterilize the natural soil to kill insects or bacteria that may be present. Sprinkle the soil with 5 per cent glue water and mix thoroughly until it is slightly moist but will still crumble. Apply a liberal coat of liquid glue to part of the base and sprinkle the moistened soil evenly over the glue; press firmly and add more soil until no glue shows through. Continue until the entire base is covered, and then sprinkle lightly with soil. When it is thoroughly dry, remove soil that is not attached. Some- times it is necessary to glue on a second coat of soil to form a perfect coverage. To make cracks in the soil use an excess of water in the soil before fastening it to the base. Duplicate the moisture line on soil profiles by spraying with paint thinned with boiled oil and turpentine. Choose subject matter for the foreground and arrange it so that it will blend into the background with no break. A photograph of the completed exhibit should appear as a single unit, showing no break between foreground and background. Background — Use a sheet of galvanized iron in constructing a small group. Nail it to a wooden frame of the correct contour; the metal should form an even sweep all the way from near the front toward the center back with no undulations. Nowhere on the background should there be flat areas, yet the curve should not be part of a circle. If the sides and the center back are not curved and are connected by curved areas close to the corners, distortion will occur on the sides and the back; in fact, the center will appear to bulge. Use long curves as indicated in Fig. 42. Clean the metal surface with vinegar and water, and then apply three coats of flat, pale blue paint. On this, sketch the scene and paint it with oil colours. To obtain true tones, paint the background under the type of lights to be used in the finished exhibit. Objects should be less than life-size, even where they occur near the foreground. If the background should appear too cold when finished, spray lightly with rose madder. View Window — To achieve greater illusion of distance and perspective, limit the size of the front glass. Cut off from view the edges of the sides and the top of the background so that nothing extraneous obstructs the line of vision. Use an apron, as indicated in Fig. 42 right, inside at the top of the glass to further restrict the line of vision at the ceiling. Figure 42 Plan of diorama unit. Left, proportions of the base. Right, end elevation. 49 Lighting — Arrange the lights so that no shadows are cast on the background. Accomplish this by using a wide area for light sources and having the lights shine through ground glass. It is better to place the lights in a separate, ventilated unit; fluorescent lights will reduce heat. Leaves from Paper — When large numbers of leaves are needed it is best to use metal molds but paper-based leaves may be used in dioramas that have sufficient depth. Use cream, double-faced, crepe paper sized with thin, liquid cellulose nitrate, Formula 45 (p. 70). Cut leaf blades from original patterns, lay them on blotting paper, and form the veins with a veining tool, copying the pattern of the natural leaves. With an embossing tool, rub between the veins to shape these areas. To make this tool, solder ball bearings of two sizes on a metal rod, one on each end. Wind string on the center of the rod and lacquer it for a handle (Fig. 43.). Figure 43 Tools for modelling paper- based leaves. Upper, veining tool. Lower, embossing tool. Attach tapered wire coated with liquid plastic to the back of the midrib, and then shape the blade as desired. Dip in hot, colored wax and twirl rapidly by the wire to remove excess wax. To keep the wax from spattering, tie a paper collar around the top of the pot. Almost any type of leaf may be made by this method. Although not dioramas, two 8-foot groups were made illustrating damage and control of the pale western cutworm and the clear-winged grasshopper on wheat for the World's Grain Show at Regina, Sask., in 1933. These were constructed in cellulose nitrate, one of which is illustrated in Figs. 44 and 45. DISPLAY UNITS AND LABELS Display completed exhibits in closed units to protect them from dust, breakage, and insect attack. Also, the exhibit must be securely fastened for shipment. It is necessary, therefore, to provide a case that is attractive in design, that has a glass front; there must be a dustproof base or a back on which to fasten the exhibit. Display Units Display units may be of various designs and sizes to suit the exhibits. Most exhibits made at the Chatham laboratory were mounted in standard units of two sizes, 16 by 19 by 3i inches and 18 by 21 by 3i inches. They 50 Figure 44 — Injury by the clear-winged grasshopper to Marquis wheat, and egg bed in sod. Plants in cellulose nitrate. (Displayed in National Museum of Canada, Ottawa.) Figure 45 — Grasshoppers emerging from sod, and control in wheat with poisoned bait. Plants in cellulose nitrate. (Displayed in National Museum of Canada, Ottawa.) 51 CO Ul o Q UJ _l - h- co — X 1- D o z < Q ui I 1- 5 t: $ o z H < UJ l- a. 10 O < CD t- u 3 cc Z uj UJ _l zz < \ Q. -1 — *Sr ■S OO oo < -I :i -•I ~<»u Q. O \ /' V)|