WALDO | — © an ears cc co Ca) Sey © Se) ~~ ~ Pes ©, i ‘ C1 1900 UNIVERSITY oF ILL. LIRRARY SGNG IS fe Shy a () SD SD B() earning and Xrbor. ; LIBRARY OF THE University of Illinois. CLASS. BOOK. VOLUME. ; MG SNe eee Re erie ae res INECESSTONN INO ie ee es ee Qe eais SE Se a sea esses Sa de ae Sst Ti ] \ i AX. OzQasQesQe seas sea e " a ® e O —] i, i Ning = a OO ROR ROK OK OK OF LN x NANT a INVB iS ey an ats, eal ee pene) i | | PROTOZOA OF SALT FORK AND ITS TRIBUTARIES | | ; er A by LA MARIE L. WALDO wall : THESIS PRESENTED FOR THE DEGREE OF . re BACHELOR OF SCIENCE 1%. Ps . in. . | a | ZOOLOGY, | ca . COLLEGE OF SCIENCE lin ys | a : ) Sea eee Pain Fa: . UNIVERSITY OF ILLINOIS ee: 1 os Ri" ’ oh (x ° is | savin 5 As Ari) . ay) a © i -..5 | é ‘ . UNIVE Rah Pray Ore RELINOIS } Fe em THIS IS TO CERTIFY THAT THE THESIS PREPARED UNDER MY SUPERVISION BY 2 ry fa error Oot hr int preterit re iter tr oer rert eer tittic ttt ttrtrttirerertttretirirt, (trtrel (Osit Cicero r DEGREE 3. Q. sretaeeeren Aarereerreensereas Seer eseursecrevarees apeaavansaeyean SevssoveeeennansneaueesnsneeevensasenesnesevevestnnnesAeunsaueseacorsrsesereneunyy Bertnr Ws AT ANASEREAAASANSEDEDSASUANRAAAAESCEE So Me UAUNRLAAANSANEERHALSUASSERAUAHAHAESECYSHUSSSNENEE SONNE: anes HEAD OF DEPARTMENT OF rv ere aan? ae 7.1) 6=2007R a ~~ 7 at i“ lk tt be 5h ; ‘TABLE OF CONTENTS. Page. ER ve aracteristics of Environment. .... ORS a6 a alsnin'aia va steams i ee ae «S06 90. mparison with Gere eerie heel elag lik aragets. aoa. 21008 eG ts cagialee > jose 4k hance ba owe median eda dou ne lOee Explanation of PlateSercecececeecccesecreccccsessscccees 106. ee etal ie sais pn -s Sine » 9) a\s'r s\ghayaie's aiaidl op winvels's s @ eee aig | 12s ce AS ona Bate ve ih an Digitized by the Internet Archive In 2013 http://archive.org/details/protozoaofsaltfo00wald INTRODUCTION, In writing this paper it was my original purpose to deter-' mine the protozoan fauna of an ordinary western prairie stream, and pe ticularly of Salt Fork and its tributaries. Later, owing to the great territory which this presents, and to convenience, the work 7 —) as ee. was largely confined to that Branch of the Salt Fork commonly cS | ae _ known as the Poneyard. I have tried to procure material at all seasons and at all “condi tions of the stream during one year. Samples have been taken qt a Boon Bis terent places along the stream, usually dipped from = _ Promising localities, where the water was still or covered with “scum, ‘or along the edges where leaves had fallen in and laid qui- . vetly for some time. Such material has usually been brought into | ‘the laboratory and kept alive in aquaria until examined. Where it es Peesible to collect in a boat,y- north of Urbana above Crystal lie. the tow-net was used. The tow-net catch was brought into the } Bore filtered through hard pressed filtere (paper which eee ains even the smallest formss the precipitate was then washed off ae and so preserved. = have also studded aquaria about the laboratory a great deat ‘These have usually been derived from the abs yard, but sometimes they contained, ’ in addition, some material Siuattt from the pide re “no is River. So the Paper contains, not only the pone sos occurring naturally Le. 2 prairie stream but also, to some extent, those peHid yy EMaviv i erry ie vr. fe We bn : £ ‘ ; ‘ ‘ 74 “3 beady) 5 belies vEM! tel’ - ane cata ot etd ir ae ae | mre 7 e i) \iveme dy spn ql And aattla ‘inetall ‘ : of il ae “ bal : “7 ‘ naps iiesal wi an se eh ofl chee i): paoke ae of fi te at sth) on ga barges oo om rot ete’ ee ! Ist Soe etets') | _— ae o? ol i. eae sebvasins | ln it Deere asah ts qhotatcdal eff PuBGe’ airiyss Poti. aéei eka vit piece c ane (edt yey? bazi5o* “4° Uy | ‘as ; ; : pus =. 7 os a io 7.¥ Sr Lili’ me! det) Feaats ies aye pitt ial Alas ; rt 7 * oe he Batarw ncn. sorta cm es, eee gundy 5 itatye athe o Sa et MALS i 1 4 , sy 5 Sea Out with a pipette, and preserved. The material mounted was kiled Pieces with one but not with another reagent. In studying para- breeding in aquaria about a zoological laboratory. The killing fluids have teen 2%-47 formalin, Fleming's fluid and 1% osmic acid. The principal stain used was Orth's Lithium Carmine. It was impossible to make many permanent preparations. Parasitic forms on small Entomostraca have bean mounted. Paramoegium ee was found in such quantity that it could be stained and mounted. om ee ee aes ee om large enough to be’ seenwith the naked eye and so easily picked with Fleming's Priidgeand stained in Orth's lithium carmine. The excess of stain was washed out with acid and the material taken mnprough the alcohol grades to cedar oil, which was used as a clear- ing agent. The material was then mounted in balsam. My methods of study have been simple. Of course it has all been done with the microscope. |] have made drawings of the dif- ferent species whenever it was possible in the time available. I have studied the forms alivé’when I could. If they moved too rapidly, and it was necessary to quiet them, one of the agents men- tioned above was used. It is sometimes necessary to exercise care in choosing the killing agent, for certain of the Protozoa will go Sitic forms the host was entangled in bits of filter paper under the cover, in order to quiet ity After the drawing was made the animal was classified. Letdy ('79) was used principally for deter- mining rhizopods and Kent (908]) and Blutschli ('80-'8°) for the Others. A list of further literature used is added at the end. % i P ' ts 4 Een As 7 tay — P A = ar c al Yi ui T pf 4 ; ’ Ls AT" i a Tae n + ra jed Oe: vale Pe Hat. acw enieatp eto liaGgirniig | ent (98 ~4 96" 0 ¢ ® i we Peet 1 eo a ha have uate) Laor bra bie anh 18 hOB 2) as vs Lidaee 3 ection E tac ele ee ngal' end ehiont, +e nae ott. bh sag Yaad asae ot aif aed wae 7 ¢ he mae} * pees re ; Hj ‘ a haw | “~~ ‘pe? ce pee ope. ae - = i, L . we . f 4) ? ¥ | Bue dova R- axe re, sett | oie ad of A r a¢feate s- tult 2* snr Ke . sy afeta Yo . ‘sto. Teheole eda! : rs hao oat o? cuit tart ts rhodiee vc>-apote od) BERRI covenotaate (le cevot aa barbuta ow 8 Ba a beaw ae rose ° Ape fead i>. i sli tee notes have been ‘Ghken eat the characters and habits of the es, and tebdrd! kept bf the times of occurrence. 2 closing” this’ preface, I wish to express my sincere Dre CoA, -Kofoid, who has so kindly directed the wortile S.A.Forbes for his encouragement and tae. and to nk pith for fais apiyate assistance with materials “i ae Behcieaga> | Veadines ey a cia Oho tintoees Ie o i nat? Characteristics of the Environment Investi sated. The locality studied is a typical western prairie with the stream, in some places little more than a ditch, winding through it. The Boneyard flhwis through both Champaign and Urbana, crossing the \University campus between Healy and Green streets, justb.ack of Engineering Hall. It empties into Salt Fork, north east of Urbana Imear the brick yard, at which place it is about twelve feet wide. (Salt Fork belongs to the Wabash system, being tributary to the Big q Vermilion which empties into the Wabash at #ugene, Indiana. | The banks of the stream are not high, having an average height of about mete feet but in some places reaching eight feet a more. They are senerally sloping, although in some places they Smpewrather steep. They consist-of black mud and clay. The bottom is partly mud aad partly sand and gravel. The banks are grass- }grown and the vegetation dxtends clear down into the water in most iplaces. In the stream are found many forms of green alzae-- Spirogyra, Protecoecus and Qscillaria are most abundant-- a great number of species of diatoms are found, and also many bacteria. Moss, too, is often found growing on stones in the stream. | The stream has been modified more or less by man, since two jcities Of Considerable size are located on it. For instance, the — | Sewage of the city have had an outlet in the Boneyard until the last year or so, and many drains still empty into it. The hot water from the University heating plant has also been blown into it for the past two- years. 2 | ge @ -S OA i aie Le - ad > 1 so ¢ 2 Wi eis ie ' : hd , ' ft! F r ‘Ss e 4 ‘nox nt dypiodtio Ki ene biel Wn TS 7 Ti F Ot 5 bt aly 20n 938 Ff Ss eT) 22 ann (ta 4 nf S 2a4 ona ssai'.% : = =i tert 154 FEF=f% lg byayenal ae ¥ 427 a ot as oi heh acne ne »4 7 Pre i ae > ea. 24 nateoe. S16 4 i ~ jet He deitod su tot etek we ~ naa we v nal ds faa = ™ je . Wa Lou te: Cea aletG e702 oe fer? @ ei stan si! 1 ao al is es 4 yy ‘cc 4 =" 80% ; Ts § a “ ia” e 19 | fal 4 = te & TL. sia Gla? = — Vane pete: sve 7 Paes io 4 ars depth Pies] I should say, from about six inches to een ‘inches. During flood, in the spring, it often overflows ts banks and spreads out for a distance of several feet on either ide. I have never seen it entirely dry, but during drouth in the ‘r months, it gets very low and often dries up save for pools lere and there. The water reaches a high degree of temperature juring August and Septembe r-- about 358° C. It is usually during @ months that the water is lowest, due to drouth. This winter here was about eight inches of water in the stream, and this was rs ‘ozen over for the best part of four months. Twice when I! colected, qéle ' fr h 1 the month of January, the ice was three inches thick. The course the stream tis exceedingly tortuous. It is a continuous pool | iach post of its course, riffles being rare. The current is very rr ae ‘aed those Jes ee inode cAnitvr ‘aon aay Tig 34 LT q9 et ai ermart git te oo.) i . egaege tn Rags fi bees gee 6 re ‘tude: «een teas lite | oe 0a # ; a Th +, mr r ) “4 € 7 ( =a] ; — ' ; ua My , i : ' ¢ P , f ~ 7 * 4 1 LF ‘ / an © - 4‘ c + ‘ A m iw? .@ a ’ 4 d ? \ 7 ' " £4 cA rR 9 ae i ee | - 4 . _ es : - —_ 4 preps 6. aA “Synoptic Key. “ “oe The following synoptic key will. serve for the identification the ‘Species listed in this paper. The classificatien in the main _— on Butschli c 80. BO) - » supplemented by Lei ly (Che aoe @ Rhizopoda and Schaudinn c! 96) for the Heliozoa. CLASS SARKODINA:- Proton lasmic mass either naked or shelted, which mq _ moves wholly by potoplasmic movement: or by the development of pro- toplasmic processes. i “Subclass Bifie peda: = mn | Naked or shelled. Moves by simple flowing of the protoplasm or by the putting forth of pseudopodia. Changeable in form, ee cif Bonewaet constant, inclined to monaxial form. e Order Andebaea : - = Naked Rhizopoda, very changeable in shape. idopodia lobose or reticulate. With or without nucleus and Bcactile vacuoles. Family Amée@baé@a lobosa:- Lobose pseudopodia not forming a net- Genus 1. An6@ba: - Containing one or More nuclei. peeudoe 1 lobose, often branched or pointed and bifid. Sometimes moves simply by a flowing of the protoplasm, without Aeaarapilnd distinct ps pseudopodia. Conteactile vacuoles present. Multiplies by division Ve i wi carigrttpy -O88 Bbiqesye bad! o) vroe Pit Sie Geran eae r ; ht wv .pmieg @RAS wt beh ‘ ’ ta 2 o. ¢ T gy *inaeeo erie . a. _ aS hire y= 6 2 “ ‘ Ln-¢ al #3 ; a ‘ Fe, ate seta hell . - . 1 / ro bi Ss . secdeaed 9 ¥Tav « pit 0" Sie lauer efAag te one tai ats? 2D we ihenge abihyd bee baimteg 1 done ha stanporg. meh: oe nt tqgo borae, ther i) Tir ede ae a in moving condition. Encysting o.served with and without repro- ji auct ah, > ' ay 1 end not villous 2, With evident peedtepodie«:. ee ee proteus Me (et aie LS ate A me eo mm ome ee te Posterior end villo_us .we..ee. yaoi: villosa Se ee a me ee Oe ee Be we peeudopodias.s..ses, A.verrucosa zp Genus II. Pelomyxa:- ~ Amoeboid. Pseudopodia very blunt and not distinct. Posterior end with villous. he hae Encloses a large number of nuclei as well as small rod-~ or bhacteria- like bodies. One species 7 ee (, ee P. villosa 4. Genus II]. Dinamésba:- Ovalywhen at rest. Pseudopodia few Or many, and everywhere bristling with rigid, cilia-like processes Posterior extremity of Body with retractile ap ieee OMS>SPECTCS VWyetcsaacrvevsess sevens coe De “ind rabilis’ Ba os me ae ee ee ots om Family Amée@baea reticulosa:- Pseudopodia forming network, and te from all sides es the body. each vacuoles many and small. Protoplasm colorless, granular and possessing power of expanding and ] & we xf . — nine itself in any direction. Pseudopodia arise from all parts | ot ae branch and Bae enges in abl Smee One bo ee giver? a6 tibnee , : Sin ¢ J ’ oie inn ine nee (i fen pibmeqg itebtee Ttiwy tT et 7 : f i a } pa (i gt j i f eee . : i eo - 4 faciwe lave ft aes 7 ! eRe Lt eet ; r ee oe f . P * ie “ on oe - cot) Ls ; air © , kan yee A & @ 4 PA 7 ; () ‘ cs oe a= & - - i; fase, + tran se legoRy atl agrie* x , - F a i ; i As & ® taeoey Martes anda bra 4Aluc m3 aixoigays i malta etfiogobiaet OA: — une at -nfeadh “ano honor! te a roe eas bas oqav’ g 7 5 a ine Bre As tieged se i ae = es pein ote tes aaines ad aca) ir a ' Testacaa: - Rhizopoda with shell of chitinous material, of silicious plates, diatoms, or quartz fragments. Bieereerinbertorata:- Shell wall solid, not perforated, 4 with fine pores. With a single mouth opening. Family frekilina: Shell monaxial, dome-shaped to elongate. Sometimes ee a one- «sided position of the mouth, it is: bilateral: = psedopodia. Nucleus and contractile vacuoles usually present. ij : Genus Vis bees: = ee ee ee ee Shell dome-shaped with convex upper side | and flat under eta in the center of which is the wide circular } mouth. Frown to pale yellow in color. Shell sometimes finely r | | | punctulate. Body does not fil! the shell. Shell hemispherical oO: Beteht half the preadth.....g. te te ath te ee ok ee Shell aa shaped Height one-fourth to one- “third the breadth. aber ohare «hie “ay seo1des Sia a eafieny Saha al ans cmt al aa Genus VI. Diff lugia:- Shell crusted with particles of sand, - Biatom frustules and other doubtful bodies, cemented with chitinous a Re aA ny - material; not brownish througout. Shape variable, spherical to elongated, and with the posterior end sometimes drawn out into gee then the form is aes! . Sometimes mouth opening is cren- I _ ae =e pl a Re el i gisotids }i8- oer - eu YO pmehart hl | Birorie " anh pee Ps) . " wire a : Ris jie « é ero A j - ‘ wy Pa ; he. { 1 ry . * | " ' } i ] Ad 1 i - 5 3 “ ‘7 é ‘ > ed ee can S «2 : lJotigeqcd tty, Satarigep ie fe tn eans woinnd: bet tarct -geA4te BME wad 3 hq) plIsinar agente 4 Weae: ; aa Ait oo eahl aanid remot ‘are sonrayese ‘ant anit of nina gees 2 ants Bade ot th go thon ania 5 nace Bete hot: cei id LAP RS WS ae pou aiiane rave 6 yh "“ BAG Lh | | ulate, toothed or lobed. Body not entirely filling shell. Pseudo- ja lobose, sometimes branching. Vacuoles and nuclei in varyang nui n pers . Ne ee tl a Fundus witha circles of spines etre 9. ih) eatewa es e without a circlet of spines. Pe Mouth with flaring collar ...... ss ss+sD-_urceolata. 11 ieic Wienout flaring collar. oe Mouth antero- terminalsesesss {rae D. constricta, 14. eis terminal. 4, MeeMeeherical or elliptical.../. SS om ByTifGRNss.i esas. be (oo ..-D. .pyriformis, 1G a — Se me ee me oe me ee a Ren ee DUM MENCHS EC Mccieta rete (os. eso cls teak se see 6 6 oars De Caneononat. ee Se oe FAR MUCH Gl cset close dia eos wrel’ oo 6s ole 2 Ds elobulosa, ee we oe ee oe Shell circubkar or brownish; oval: —o oo incorporated with particles of quartz. Mouth ‘rand fundus eccentric in opposite directions. Mouth round and infe- a. Fundus with or without conical spines. -—- ne nee ate te ‘(With conical chitinous Ore Tea Ons te .++C,.aguleata. 15. ped edd pag all acne ay oe we ee Family Rug lyphina:. ~ Shell chitinous or silicious, budlt up @ ’ | of pono! or round plates. Monaxial to bilateral. Pseudopodia thread ee es, eo ero te but little: Form somewhat long purse shaped ! i 7 7 ¢ eed! 16 Smid . ce . QuttometT Teo $99SR SEE é 4 fe Se meet 6, OE oad » * ’ J F a | nm é ‘ ° a er ° . * * 7 ed . = 1 ’ * y me al et —<— - = ae ‘ ty 7 el - ; ‘ ? ‘ a0 Ads Lp es ‘ “+h: in oe 1 | ;oe ‘ht 4 q “* ‘ = eo) Pe oe q+ 4 ae Yr. i] ' i ‘ > 4 4 =e > é ' Oy ee: § 4 - TY. . ire ° ‘“ nl i ’ : ry t2 : in) Pisce ; oi (vente Cid 49 Jeiwehe®..cethsT Sous : f ) Pie: (ce tyh ped griacnn? ete yn LO ~ im | ol : ; = e 4, . Pi rr h - a JS i859 x A, | ; “49 ee 7 ‘aa heaess atte gael | eg prot one / £ ae 7 em) 4 . ; ie a oan Oi | - ¥ . a tos a ge . : . N ; . ; , ; ; ih a . rs 4 : , - a i — oi 2 | on 10. | with st aight neck- like mouth or with mouth pushed to the side. he ‘ Shell chitinous, encrusted with foreign bodies. Posterior end | with horn-like processes at each side. One PERC IES easasawics oaas. lle te en ee Family Gromiinas - Chitinous, us sually structurelessshell, monaxial to bilateral. oval form, with rather narrow mouth opening. an I. i Pseudopodia reticulose, thin, thread-like and pointed. Genus IX. Pamphagus:- oe shaped. Very delicate shell mem- brane, Closely applied to body. Pseudopodia long and branching, arising fcom the broad end; nucleus large. - One SOS CWIES vues 6 ees eee @e ee wmeaeesee e PR. mutabilis: ia ee ee OD ER ae We So ee ee Ae Oe Subclass Heliozoa:~- Naked or encdosed in a silicious skeleton. Al most regular spherical form. Pseudopodia fine, more or less rib i. and but little inclined to anastomose; radiating from all sides of Behe surface of the body. One or several nuclei. Order Aphrothoraca:- Without a shell save during encystment. Genus X. Agtinophrys +- ee ee Without a stalk. Ectoplasm and ento- lasm not Clearly distinguished. Pseudopodia with granules and with axisthreads Which reach to the upper surface of the central nucaeyes One central nucleus. One pulsating vacuole at the aoe One oe ae Y iGO 8.0 0' ig *) e e Ye ae or Ghee aestetefass(-as toe Pe ee a ay oe ; oo. . ; ; m3 a) ts ' ve aT eC ihre 345% wil Laloun ‘septat ater | - -eented tie bo thie site .cuetaa wr fo Cees agit * 2 >» my eyse xc ¢ rot ads Tee Ai VaR. a x 4 i) 7 ; = S487 od /aanga . +=: ' §i< % ‘ * ‘ ; ie oBA eeluntcd, «ne ee eee Ba | ee » ‘ : . “ eee 7 | w Si 8 qatt St 4 i i vst f AX 7 = af? Jor eves TIQQy. BAY 2) AOEae ded 7 ee rloupet jetsoniva ant age ‘a oo “be s . sh « « ae) = @e48 84 Pe sored Ce Med _ ; edd i - i %. ir is ; —P e bist s , } ae ay n a ¢ 7 p : - eee Se ee oe ee —— Genus XI. Actinosphaerium:- ta No stark. Ectoplasm and entoplasm | clearly distinguished. Axis threads of pseudopodia end at boundary | between ectoplasm and entoplasm. Entoplasm with small vacuoles. oer “AO eae =| Ectoplasm with larce vacuoles, several of which are pulsating. Sever i f { ai A | . oa nuclei in entoplasm. te te ee tle Ae Oa tk Ole te A Te at ee wee One species. eon seen eereneeoowre see ee eee oe oe Poy ae éichhorrnii. 2g Oe Body amoeboid. No distinction between ect “sarc and endosaro. Protoplasm vacuolated. Pseudopodia rising from Peieetaes Or only from one-part of the surface. Some times with acute-angled branched ends. One or several nuclei. Large contractil¢ vacuoles. on _ BOE SDECIOS cesses ccc c esses stendeeell. DOlypOdia «lau. Order Chlamydophora:- | Having spherical, soft, gelatinous shell, often containing foreign bodies. ee ~~ ~— | i. Entoplasm and ectoplasm rather | clearly distinguished. One nucleus in entoplasm. One or several | an | P ulsating vacuoles. Pseudopodia on all sides, thin, with streaming o7 \ of granules. Surrounded by 4 spherical, rather thick, gelatinous a , shell, which is hyaline within and granular without. Surface pro- :H.smyriapoda. 21. Order Chalarothoraca:~ | - : With spherical shell of isolated silic- im + ihe * Coast ne Q staoatt hyd vada inaat ‘ po Pr » tanh ge ine - sohtabral i ' CF — 7 ‘4 ' 2:97 02 «3* a » TRE cri 4 os jotoe ee ~e one ro. — ~ ae a? es? y ry seit e ” ei sn eit A fe, 2a at sonusyt 26 haeeee word? 4aare ciastsengy * Kat cuotaie : ee } ; ; » epee Like : saa hl BG ‘qgaxtS bné Gs ¢ a ants “yn hl | Age ’ ' ‘eAgee tote Wtteboet, i fet! At hae. ad. giant tom tft sss is ; Pia tees netaaeetes ‘en losnges d y2 : é 1: e oat oe ae emi a He hs 12. ious particles, which are pradiaed by the animal itself. Genes > ae Acanthoomstis: oA ee ee ee -—_— Ectoplasm and entoplasm clearly Gistinguished. Entoplasm eccentric, finely granulate, wath one nucleus. Ectoplasm with several small pulsating vacuoles and fre- —_ S| quently with chlorophyll bodies. Pseudopodia fine,with axial threads which unite in a central body. Shell skeleton of eee spines. ONE SPECIES. ccevscsevrceevesseres 28 re eartaees: es Se eas a anes alin to nes ae | One-celled, more or less flexible organisms, | bearing one or several flagella which aid both in movement and in the eeprure of food. » May or may not form colonies. Order Flagellata: i | Having one or more flagella at the anterior engof the body. Mteardar Rodina: e | Small; simple structure; naked, often more or less amoeboid, but sometimes with a shell. Usually colorless. | One anterior important flagellum or one or two small side-flagella. Special mouth opening sometimes lackings sometimes present at | base of flagellum. Never a well-developed pharynx. | Family Rhizomastigina:- Simple, mouthless forms with one or two | flagella. Sometimes presenting Rhizopoda-like pseudopodia and | eometimes, heliozoa-like, Changing from a flagellate condition, ot pseudopodia, into a sarcode condition, without flagella. i a a4 be He sybe ca ew ‘ie ite seatt siiod th 0 |= le SSS SS rv. S be ; 7 a = r o + e y ai ; . 1 yy : oi i AJ sy i . - - | “akkearaatios cal ¢ . mie ° - ; ¢ a @ al 4 -, f ‘> = P a Qi a < 4 4 f] ; Art i = a Pps? ae : . . aie - . 7 oot eq ser thangs ggtitgel wea! tusce erteegaenay = 7 si ‘2 La ret ede bede leweb- lio. « ad ota Prey | 781 aq A On, pigal® - oer 4 +) 5S T antpcergens FA gon tahoe @ sh ie etait tute! rae: _aieane te eit ten hae a. = — re 7 ig > 4 a ie — arn = ety , 5 te ahenan j f ad 5 @ 300 aneers i slave ; ; i, < ofe Suliege(: sco breed Salas he eee =af YT a6? 15 e249 eyit b2@ ied ye oiasticn @. el aclted 40 Sane Se? ‘dome (hed ig Th 2 Sam baglilaseese sh On heed 4 str-914 4s Le BESTES — it | Seed “- ‘en (rah ewe ouk action es. aetio ate, c f . 24 . aE af a fh ‘i » aye - t ri Lf > Ae arg? eo y A 7 - *, 7 Ts a oa Fee j ery IR ‘s bad ate = i Colony-forming; shells united to “| each other or to common pedicle by slender footstalks. Bodies ovate. | with projecting lip-like anterior border. One flagellum. | One species.. UeRSN ata NG Caie Rie elela cise 006 4,0). ep 9 00 De ADwOLt A. eae femity Yeteromonadina:— Be) +, Smally colorless monads, distinguished 8 | | by a prominent forward flagellum, which is usually accompanied by | fromrone to two small wavy ones. Frecuently forming colonies, and then with a stalk separated from the posterior end. Subfamily (oso ee Characterized by lack of colony- | building, and also by occasional increase in number of side flagella ‘Genus PO Lele Barenonas :— Ovate Or allepuillen: Gnd lapedle eee | more ‘or less persistent in shape, incepting food substances through Mia Gistinct oral aperture, which is situated anteriorly at the base he eh oe me Om oe | of the flagellum. DE PSD OVES cise ss 6. ere.) «aie fe) waiee atures &LODOSA. 26. Subfamily Fondromonades:- Characterized by formation of col- aif ( menies, and also by the fact that there is always kut one small mY flagellum. } Genus’ XIX. Anthophysa :- 4 i : a 7 ; : ! Cone-shaped with broad truncate 2 — a r es ‘ f \ | Peristome process. At the base of t his arises the main flagellum || i anterior end, and produced on one side into a beak-like pointed a al! lees ; =v a) i pay aeigie’ FYE ' Vago | | shcela (i Mbette, ghemeb Airy Rit: 2 oc0 -yehred gee ie ent atl-3Mais ¢ ae a * e a ‘Gs x > F ’ Ls 7 5 $2 j 2 4 “ ; MO 2eaivd. a ry * rn” = j 7 \ a ; TL imply = oe AW. a » - Lon of? by Pd ‘te as) ’ ie swtiggel f = : ¢ ‘ ~pereigg” bh a cuene 2 | 2 > ; ' : A - 7 Esta ite “OQBik « ORO.) : 3) eee 4 | : cet ial ding ath? Atead «4 fal abit one to agree ary piel? cham. ent foeiie 1 16 sae: “etd ik A ; eee hl “tn nie ‘/ f an ° \ os 4: 5°. “oy -y | ~~ a = a - ‘ ; = 7 » ty j a ; = a ‘ 1 a! _ a =e : | nd besi ide it the small one. Nucleus and contractile vacuole 4 By asterior half of body. United in rose*te-like clusters ona z | ee tenously branching stock which unite in colonies. PMG SHNECICS ccs rvevectacosese Ae» vecetans. 27. rap ek lS ed Suborder Euglenoidina:- | Usually amoebold moveren echs PrPaSeT ists: “Slightly asymmetrical. Contractile. Colored or colorless. Mouth | opening just back of base of flagellum which leads into a more or ; . less prominent pharynx. Contractile vacuoles near pharynx, and some- | times with reservoir. | Family Fuz fhina:- ‘ Body monaxial, generally inclined. to bilat- | eral symmetry» since the mouth is a little on the ventral oul “Elongate, and posterior end mostly sharply pointed. une surface | more or less obliquely ciste: Metabolic. Small mouth and strong | tubular pharynx, from which the single flagellum arises. One to | | several contractile vacuoles. Body usually green. Prominent -_— Sse Without distinct shell. epindle- shaped to eer needle- cae: and with pointed posterior end; or cyl- indrical to ribbon-shaped. Very metabolic. Oblique striations very ff Jr ine. ‘Chromatophores usually numerous and disc-shaped. Mouth [and pharynx usually well-developed, with flagellum arising in the. | pharynx. : ee | Sirrace epirally “beaded. ....2 «ese . _spirosyra. 29. Surface not spirally beaded oe . es ie a a wi a Y bs e.. e's vets Plea 9 i; sot bp Uae lay ube re) het - 1 ay Weete, ud): +0 gr itonaed cts = 238 ‘4 er a S65) > 0) = z 3 v: 4 ’ a : sl ni ; mute re videdernes ¢! ie 4 * * | ® » 4 ¢ irs ar ; > Site f rn! a) ena 3 idg eel @ treact® aleni? oi? A ote t | ect jan ‘Pave qooe eae bt} Ont eae bod) le it ie + 200 goa) J uae ~- Lent te = —~ wo ‘ “ 2 4 ni Pals q 1g, So 709 J Le ae ‘nm, eis aieow peataboe 4ftecher: Hsogndacetbsha | - s@ aol edo. siidalates qray .gatedae noddin of x 4 tic? Mabey oe DE ema Suet amber dl men otiagh J re if aia rs , ive Mali pe est tte beep toned cee its ap : Fr’ 4 ade | Wi . Sallie i Be a ee = ot © — oe 16. {Anterior end tapering, abruptly truncate. Posterior end OUNCE. cele cl os cca mates se. eee BR. aCuse pe Posterior end not long acuminate Se Posterior end abruptly acuminate. Somewhat rigid. sooo ek OXYUrIS 50. ee ee ee Posterior end gradually acuminate. Very mobile. ecsvees Viridis. 28. Genus XXI. Trachelomonas:- Structure essentially as in to brittle, colorless to brown shell, whose shape varies between | spherical and long Gore che pedk The shell has a small round anterior || opening, from which the flagellum protrudes. | Surface hispid. Without tail-like process. | My QGNPes hispida. ©! 5% Surface smooth. With tail-like process. -+.T, acuminata. 2. Family Chloropeltinae- General structure like Fusenina, ” = | but different: in the strength and resistance of the cuticula and by the almost complete lack of flexibility. Always a prominent | caudal process. Sometimes from one to several disc~shaped paramylon | bodie S.° @ “Genus XXII. Phaous:- More or less asymmetrical, flattened, 7 elipsoidal to pear-shaped, with a more or less prominent posterior | i ail, which sometimes increases the asymmetry by its distorted 1 + an j i - a lade ; y ows ,000 of I sbtQea ‘J - a tJ 3 ty - iy 7 , . re si ié iia, ar | 1s —ne r re | ’ tetsod i stk0% \"q I i) | . . Bee ien | és i] ri taee , a “rely 2:04 LeQoote % A UO yi 9° ( aepesroed eon) fecoe folie Sane + | iin 17. | position. Guricular surface longitudinally or obliquely striate. | The whole body sometimes twisted. like a screw. Usually one large paramylon body in the center, back of the nucleus. F Caudal prolongation short and curved. P. triqueter. e4. Caudal prolongation long and twisted. Peeecoee, Vongicaudus, © Bes Eemils, Petalomonadina.- Blane ce. Wit constant form: somewhat oval and flattened. Large flagellum at the anterior end and close behind, on the ventral side is the mouth opening which leads into a poorly covey oped pharynx. @ Genus XXIII. Petalomonas: = | | Oval, very much flattened. | Central longitudinal furrow. Very long anterior flagellum which is vibratile only at we anterior end. Mouth on ventral side, at base of flagellum, with very short, if any, pharynx. One contractile | vacuole in anterior half of body, to the left. Nucleus to the right. GE MODE LE Shing Wisi ilele es occ se eee se P. mediocanellata. SE. Family Retostine -- | Colorless; flexi bleror rigidy simidar in A structure to Euglenoidina. Butschli reports a side flagellum. Bl ther authorities do not. : | Genus XIN 4 eee = Elongate cylindrical, pointed at both ends. Very strongly metabolic, even while swimming. Mouth terminal leading into a well developed pharynx. “Ti One co oole Wen a oe ei oe tricophora. fee ~~ ee oe mee ~-~—— = a ~ ae 7 - 4 . 5 ' ni _ -) 1G = ‘ @ - A ae a oa OP o- s Ay rm Li ( d a aa ri ; F y ‘oir Pa ae ; : 6 in : » he 1 Tee (toe thioe igeand, mandarin ie oo mie he eae savtinnge ad? Je Raat f6ba8S ont at ‘a, a it. fy ‘potted Tape: (Se eRe RS | ‘bre Spat met fcinoiatay oon ee ; atay 2 ¢ 57 » oar ‘sé g , ie) 7 on; f) ' a ) Ba tie : : ep? aan Tt ae salagnl ilied} rs _ } fey in M 7 v1 66 in slawh et Biz iy ‘ te )9elae tie } aphes . ; os ee eee ant | ; 7 ; p . ont | anaes >) afteper tlete lS: «alata sii? Ade , ore OF O88 e! (aptauie Sige etagneia aio jw Sith, ale mets aa wien _ i ot i Ba: Genus XXV. eee tena’ = Ce ceclhenlheiendiienatsttenlirantiae Slender, elongate cylindrical, tapering to both ends. Side nae ae prominent, extending poste- oe ee ae we DISC CMOS sn wesc swe be 2 e's cee te cee e ee Mee Nee es © etic lly meter ——_— Suborder Heteromastigoda:- Naked and becoming amoeboid at times, Or rigid and then Erecuyatly possessing cuticular similar to Euglenina. Two casas one is pipeoted forward and accomplishes forward motion: the other and longer one is directed btackward. Both are attached at the anterior end. In some forms the posterior One is increased to two. Mouth leading into distinct pharynx. Family Anisonemina:- Oval, persistent in shape. Body flat- tened. Bere ide rable difference in length of flagella. Distinct mouth opening at base anterionly dineoted, flagellum, on ventral side, in connection with a long tubular pharynx. Genus XXVI. Anisonema: - Pe asymmetrical; very much flat- tened. Delicate spiral striations. Trailing flagellum arises from mouth opening and extends around the anterior edge of the body mm a Curve to the right, and runs back, down the right edse of the tody. One contractile vacuolé sin*the anterior end, on the left side. Nucleus round, behind the center of the body. One BD CCd Oils de ane iss oe ep ees Felt, ee0ne39#ee#e?#e Aue zrandis. BO Suborder [somastigoda:- coh Lorne. Anteraor end with 1, 2 a, ‘ih se _¥ -" ek, reba’, cn ,idenicorg mahiaeaae #bi2 « Stine dtad. t | 62esige an ol ode ° se . . sr es * &e eae é ep e 2etsaqy end Al . = i ebop!tectinteten eae : oe f Peer zy ~ ison an? ll P: ; “~A . i j . =,* | ' ’ | 711% ¢ +. it mibsei 1 _ nv fuse. pe tsatae SA) wPawi Gort Pb Loney Yrrod. Wn oF es" ae - - } x » are ; = : rs oho wt Ye tataea ed? baided «bruor AAs ¥ " ‘ ' <2 uJ o/ mee ea @ t s'sa'@ 28 a oe | p uw iT ew ge aaupe xbes esr Tous > Lie ate at to laste ee * ugly Wie aee crea P| slash « Hae t j > |} * J i i 9 al 1 the 6 iv la . * * fant ie . ‘ : 4 . ‘ te Pa oY t78 petq spite witol eaaat ie lua ee unuel ectertvn sae ra ui | ‘Order Gymaos tona ta: - r | .. | Mouth round to slit-shape: closed. Without uadulatiag membrane. Cilia Surrounding mouth somewhat modified. Pharynx, if developed, never ciliate but eenerally provided wit ‘little rods. : Family Enchelina:- ssiort to lone forms inclined to bilateral] ee Muth always terminal: usually round, sometimes slit-like. | Pharynx, if developed, a Strai cht tube directed. posteriorly; ei ther short or lone. Subfamily dOlophryina: - Whole surface evenly ciliate, that around tie moutr beine sometimes muci larger. Toe cilia is ocea= | sionally limited to tre anterior half of tie body. Genus ASQVI. ‘Enchelys:- et ee ee ere ee wee ee Small, wita anterior end decreasing 7 S: i. 2 20 size to form a neck-like proloneation. Posterior end rounded. .. | Anterior end truncate and Occupied by the mouth, which is usually | Surrounded by a Sphincter-like lip. Cilaa short and fine with a row | of larcer cilia around tie mouth. Subfamily Colepina:- Peet! Pe Peta: body short, shaped like a small barrel. ae anterior end broadly truncate, and wiolly occupied by tie large ith opening. 11e€ mouth ig surrounded by a wreath of larger cilia. Ite rest of tae cilia is ratier scattered or confined to tie left | Side. Genus XXVIII. Coleps:~ -—--— = — a Mane o ta aos .% | " Body barrel-shaped to somewhat flat- a ned, and generally a little curved to one side. Posterior end | i rounded. Anterior ua broadly truncate, wiolly occupied by tie mouth. GLa iit-ea mn «a sf bet 1806 (20 LTA aay. 1S "~ ‘ r ar Meentamar gee oS mt | val geek - ee pert: it SEAS. oe mee = ie / z 4 0 ¥ } Pal i Pi ’ \ he 5x ue ; } ep. F 3 ‘ } ' < t 4 @ wetetatic? 2 ie eee = - a ae get Me eae mand ‘ae - “ah Py but slichtly resistant. Simple contractile vacuole subterminal. ves quickly, rotating on long axis. Shell made up of small. pieces OwaetoOeetaer, wiici are arranced around tne body in four chief | eircl es. Re PP Ec wine wis olen es eens en hirtus. Bl: q U000(@Eeeec Toe ey fF tsk Ree ‘a iz Family Trachelina:- | Body either bilateral or asymmetrical. o Po. ES, ; Seenoom twisted. Stronely compressed laterally and witn left side la : more arched tian right. Tne mouth is either a lone slit extending |from Siemomtertor end posteriorly, on tre ventral.side of tie pro- | jecting process,or only a slit-like or round opening at its base. | Pharynx lacking Giecherd. Cilia recularly distributed, or limited ta | the miat Pight side. i Subfamily Amphileptinae: - | The mouth always lies on tre convex |veatral edge of the snout, waicn is. bent dorsalwards. Sometimes it fi as a long slit: sometimes a round opening. Genus ZXXVIII. Amphileptus:- — et ee ee ee ee ee sac-shaped wita snout-like pro-- mecti1on anteriorly, and senerally more or less compressing tne ante- rior end. Contractile. Tie whole snout edee encloses a lone slit-like 5 | | | | } | | pou ta. No pharynx. One terminal contractile vacuole, or numerous petered ones. Irichocysts sometimes in tie snout end. acronucleus JbilObed or in four parts. One Species ee ere eee oece eee ew eee eee es wee eee Avs auser. See ee Genus X£XX x. Lionotus:— ee me Comtraetile: shape essentially like mb Lepuws, though usually longer and with a much longer snout. —S ee Oe ee de strongly arched . The snout and the body edges always compressed. | jltouth as in Amphileptus. Trichocyst® alone ventral edce of snout. le eed be ‘hall +b sr ee ace rite tree 7 | Ter hat deit tue SG0ne sll tet ra ah ny Ge east BF ty att Along tie mouth edge there is generally a row of large cilia, waicha | forms a sort of adoral zone. Contractile vacuoles, one to many. | Movement, sliding and swimming. Macronucleus bilobed. 2 fatieresiou Saort, OGhtusely pointed .Neck region less than Srey iie, wody eNO TIR. ... 5... 2.2220 L. fasciola. S05 Mie weneti OF ntie-body......-..-..- *.Lie wWhzeSsniowskitwos ae — — ee ee rs Family Chlamydodonata: - Oval to kidney-shaped, never very lone. | From rather round to flattened, dorso-ventrally. Mouth always far ‘| toward tie anterior end, sometimes in tie middle of tie ventral side, and sometimes placed to tie rigat or bert of tae middle. Pharynx | sual ly wita rods,, but Sometimes it is a smooth tube. Usually gorged with food. Subfamily Nassulina:- Round.or.a little flattened. Cilia | on all sides. Genus XL. Nassula:- 9 hee nee ee me eee Flexible to somewhat contractile. Ege- | Shaped to eloncate: sometimes flattened dorso-ventrally. Ends equally | rounded. Mouth ventral, some distance from thé anterior end. | Anterior end bent a little to the left. About tie mouti is a row of } strong adoral cilia. The remainder of the body evenly ciliate. Stria- ‘tions delicate and weakly spiral. Mouth circular and pharynx | rodded. Pharynz Usually Extends to the left and denusaiiv. Contracts | vacuoles changing: sometimes One in tie center of tie ventral Side, | sometimes as many as four, some on tre ventral, and some on tie dorsal — a et side, Usually a complete coat of trichocysts. Colorless or cotored | red, blue, or brown, by pigmeat. Macronucleus spherical and centra). | _ One spe@ies....... ae ed N. rubenas. 35 SS Th ee &¢ Oru ( 7” al el | ee. > or | i! Jeyesat, . snmt feta ty 49/1 wy eet een Tes BOP eal , é sfe er ve ' oT - a Pres i if ine J 16.90? seen, oe |? Dey meee & ae issiiey ore Se) @2 lies Ree iebak Be ii al ees is. wg , Nodes a eiaiens «5 . ae 7 & bP at ee Subfamily C1ilodontiaa: - Stronely flattened. Cilia confined to ventral Side or else muca larger on ventral, than on dorsal side. | No distinct caudal style at Lie posterior end. Genus XLI. Cailodoa:-— ' Persistent. ec =shape. Flattened dorso- | ventrally. Anterior.end bent to tie left in ratirer pointed beak. | ventral Side is slightly concave. Posterior end broadly rounded, “surfac € finely striate lonecitudinally. Only tie ventral side ciliate. Cilia thick about mouth, formine an adoral] zone. Mouth median, in @aperior Malf of body. Pharynx rods well developed: straight or wita ‘ianer ends spirally rolled. Contractile vacuoles varying from one to | Many, increasing in number with size of body. Macronucleus oval, | ceatral. | eee so As oe ocpge ste aie ee slo Ceecucniia hus.) eee Order Irichostomata: - | | Cilia of the body of very different kinds. | Mouth, ae a rule open. Pharynx uSually developed: tubular and open. Mareia Of mouth with undulating membranes which Sink into pharynz, ‘or pharyax provided with undulatine membrane, or with cilia which may be derived Promine adoradzone:.. . e Suborder Aspirotricna:- More or. less ellipsoidal to kidney-— hape: almost always distinctly asymmetrical. Mouth primitively la ‘Tongi tudinal ventral Slits, but usually an oval, kidney-Shape, or rescentic Openine, more or Less removed from. anterior end. Pharynx lis @€ither not developed or is a regular, rather long, smooth tube. PI haryngea] rods never present. At tae edge of tie mouth opening or Jin tire pharynx is one to two undulating membranes which move like lipsd | | which are not evident ia smaller forms. Mouth in anterior half of body. Pharynx | I Seiner Scarcely developed or short sac-Shaped. Undulatine membranes jj prener at the edges of the mouth or deep in the pharynx. Feristome | field leading fo tie mouth, lackine or Only poorly developed. bi. | i fenus sul. Claucoma: = EBee-shaped, rounded posteriorly aad Beiutle pointed or less round, anteriorly. Dorso-ventrally | Peete ttencs. Finely and evenly ciliate. Mouth ventral, near to ante- eens at Limes Shoved to the right. Anterior end sometimes curved foette rieht. Mouth trianeular to half- moon-shaped. At each edge of mouth: a Strone undulatine membrane, the two beine unequal. Pharynx Mardly evident. One dorsad contractile vacuole, céntral or sub- PeeiiiaieeAt tLimeS, a thick coat of trichocysts. Movement rapid aad meeady: Sometimes gliding Onvventral side.Macrcnucleus round, central. Bre OU ee Mee ts waves a 3.9 Ss GC. Serntillans. a7 ead Gomis xuLil: Frontoala: ee et ee ee Elongate cylindrical, with evenly rounded OF somewhat pointed poles, the two differing somewhat, at | times. Sometimes a little flattened dorso—ventrally. Somewhat con- | tractile. hve lly ciltate and regularly striate. Ine laree wide open | mouth in anterior half of ventral side is an eloneated: oval shape. | % tre left side is fastened one undulatine membrane, which may cover | | | little differentiated from tie body cilia and whose lively motion give sthe whole mouti amerim.. On the right edge of tie mouth is a small trichocyst-free field, upon which is a row of cilia, which are a | then tie appearance of a second undulatine membrane. Pharynx little | develoy, beh: Usually a oUlplete cOat of trichocysts. One-or two | | Body colorless, or screen from Zoochlorellae, or brown to black from et ee -picment. Pentractile vacuoles oa tie right side. Macronucleus oval aud central. Une Species’. Ja... hy A eater oe eee nacuni nate. O86, ee ee ee -7h@ Tara “oe Piccterst | yy + > Aa eet 4s “welts b Tm! Bin S ! > TBA (. é 1 v2 ee ; Pil & fee ay 4 on ia b's ; | a i A iy: . ; ‘ Rive ull hie sr be eta! mj eob* bop ekt be _,» Genus LIV. Colpidium:~= ‘Oval to kidney-shape. Somewhat Pioxiy. miterior end not so broadly rounded as the posterior end. ‘iow th Pemeererior Nalf Of body.in a. transverse depression upon thre } entral Side; leading into a long, tubular pharynx; mouti triangular Jor erescentic wit) two undulating membranes. Tne rieht membrane | extends far down Pimoettespmarynx and appears to. be fastened to its | anterior or dorsal Wall. Siriations in front of mouth twisted to the doiert, fecisteuione Of time right side run obliauely from right to Piert. Onercentral or terminal contractile vacuole. Macronucleus | spherical. | Jods) SI GG oN a ara eae Cancel pedan Boke Family Paramaécina: — }. | Moutna sometimes in anterior, sometimes Wiu posterior half of. body, wit a triancular, flat oral groove extend- ing to it from tie left anterior margin. Pharynx tubular, rather }ong, } wWita longer undulating membrane or corresponding row of cilia at- | tached fmeraorsal Wali. Cilia thick and recularly distributec. Genus xLV. Paramaeciun:- ot ae ee oe ee = — one pew hee ee a | lexible. Longer than broad. Dorso- ‘ve entrally flattened. Ends rounded cr pointed. Mouth laree, oval, eo te center of tie ventral side, with peristome field leading sto et: from tie left. Pharynx rather lone with one undulating membrane | its dorsal edee. Usually a complete trichocyst coat. l,or more of ten 2, contractile wacusles. Macronucleus oval, central: 1 or 2 micronuclei. ; 4 | | Me DSC LES ste Late cieeis a cis eae usyniata ae ee Bes caudatum. 60. ee ee ee Family Urocentrina:- SiomtiMoin tie meddle Of tre vyentral.side, AY * 1 Oh Ar Pew ! r ' 7 ~ 4 wTT) r Me lave 1 7 ‘ } ab aM oO ef", as >» al ul *'¢ oo ae iF % d Mabe teapiiqe® yn cg ——_ eer in ~ to io two broad wreaths, one in the anterior and one in tie posterior halt of the body. Genus. XLVI. Urocenotrum:- | |} | poled ples ert ce peor | Flexible. Somewhat cask-shaped ita proad rounded ends. A circular furrow in the equatorial recion | Mimrch Givides the pody into an anterior and a posterior half. ‘The farce Oval Mouth lies in this furrow on the ventral side, and from it extends a lone sroove over tie ventral side ar the posterior Mali, tO te posterior end. Atoyé the mouth a circle of delicate Siesurrounds the body. A broad wreath of strone cilia encircles th ) Ody in tie anterior half and another in the posterior half. Caudal maerenoroject from tae ventral furrow Pharyuxs Lone, a row-of tamering Meeeed cilia at tie ventral and dt 1a! = Af | J i diet et mS | . cts Ai | en tl - etl Balt, g j /& ee ' fhe “t | ios | | E my pbane May be present at ire richt edge of the peristome. Pharynx | x | tubular. Genus e On LAGS GE Aa a ge ee Ha. srandinella. | —C« On ~— wae tad a . a) '.- 2 p hited ' c 7 ‘ot Sf Saeeoe colt (Ele tae it (poad bien ai ii 0S be nS oir rt oO - eth iJ J pte, 0a2 ait Woe P80. liz 4 Uline 6) ey AX, fe sisted) ti test ns OSeere a 4 + 1 . } oa 1 40. aS Daher have appeared and age sappeared in the aquaria tiroughout Dae year. A collection was seldom made in which they were not represented. schewiakoff ('93) reports this species from Asia, Africa, Austrailia, America, and Europe. oh Amoeba’ verrucosa Ehrbe. Pl WW, WFic. G-/7. Leidy ('79) pp. 53-58. Pl. III, fies. 1-38. This is a somewhat slipyer-shaped animal, with the broad, blunt, thin end directed ahead. According to Leidy ('72) and others it is Smaller than A. proteus. The most of those which I observed were See ee —_——_—— Quite as large as ie pooteus «lire, protoplasm. like: that of the other “pecies is Pe tchcoke: eolorless-and muca vacuolated. There is a very Sharp distinction between ectosare and entosarc. It moves ver. Slowly and does not put forth such distinct pseu- G@opodia as tie other species. It rather exteads the whole curface of ectosare and then Pustecr up, Inve, tt. It varien considerably in shape, thouch owine to this manner of movement, not so much as the other weesnucteus iS round and is venerally posterior and a little to one igi Or tae center. Mumenconiractile vacuole is usually single. I1t.is large and Ound and is located posteriorly. Its food CONSTSts Of aleac, La ee aaa One large eae Division and encystment were not Bey Like tne other species it has occurred and disappeared in the etatory aquaria tiaroughout the year and has also, been found in nost coll ctions. Sehewiakoff ('93) reports the species from Asia, I iy eer Ure (DSTE ‘? Shae gon je 2en 10 ae ‘eis Pier | tice " , 4 i ~~, SLosos One Bry ™ : atrods 2th ! ’ ji 1Ss = ' + ew PTS, Throat ee | ‘ve tee, Se - Thy ors 5 df _ (éfonse: % ° MO Sb aia - 1. a i lee : ae what hontai x a Atipbarets nt on Wong pbag0sidi bolic! © see 184fG Tor Sic. sve teas ry avnyoSo ter it sn kas a (ae a, be voltae ions cu") ic ey win 52 3 els bea, Australia, Oceanica, and America. 5; | PL. VAFig. I-26. | eo evo upp. cesee. Fld, fies. 9, 10: II, 14-16: VIII, 1-16. = This is an elongated ovoid form when at rest, broader at the an- s da a granular aad exceedingly vacuolated entosarce. Jhe protoplasm is very MObile so that the animal constantly changes shape, muca resem- dling ; The animal may have a great number of pseudopodia at one time and exteadiag EREom any, part of tie ere nReLy althouch usually there are but two or three, and these are mostly anterior or antero- ) dateral. Tne pseudopodia are never lone nor pointed wt are simply blunt Extensions of tae ectosarc wit the entosarc running down into Mem. They Serve for taking food as well as for locomotion. ieee is one almost spherical nucleus toward tie posterior end, al though Often this can not be seen in the living animal. iMiewsontractilevacuoles vary in number. Sometimes there is, but miewecna sometimes there are several. lhere is usually one large one rd tie posterior end, and the others are scattered through the toplasm elsewhere. They pulsate rather guickly and attain to guite a size before systole. | Their food consists priacipally of diatoms and otter algae. Ihe fcod is caught. by the peeudopodia, the body is folded over it, and the protoplasm ingests the part needed for food, then lets tne re anne. WN ? SGORE Seton, .% .° }2R0R Wee) .cz i: Pe a Wy & J [hve8o Sree si. { . ; sco raw A ay . 0) TSS .y ; ; F- f : + iG ot A +f . 7 - + rth oad a ey ’ feo ht) Baro j mer 2 -creliiceaie rege Le Seals < 2am Teoh . is B5My 1 b» , te) 0 SS eee ae ; sm = P rm 7. 3 i ‘ Mee 11a Tt bh S785 Le 5 rsdasaf > ae ae Eee ey. 7 1-9 Gi ei ou 114 SI ri ah te, -ied ‘hh, mabOnobis:; oct ef tee * ea > <<) eee 0 Pebdpe 5: 2) Sree : 7 - = 5, 7 riot, . ene ong Eas. wap seen ine ae | eps Je et e) "ae a . : - _ d lieu et 473:60-4, iq ’ : 7 = ea cL ‘ ae. This species has occurred and disappeared in aquaria in tie aS h Boratory throucout the year. In nearly every collection there has — Seeeteast a few. Leidy ('/o) reports it from the United States. : 4. Pelomyxa villosa Greeff. PL.V/Fig.27 2a eenenro pm. Cocoa hs Vs VIII, 31-32. Bitsenli ¢'80-S8) Piterl lea file. Oe A very characteristic form in the species I observed, was an elo Sate one with two posterior horns. It is brown in color. The specimens erved, by me were very small. It. is amoeboid in locomotion. The Moearc iS a very thin film, and is thrown out at times for great Bemce-. Ine eatosarc is very granular and very flexible, and is not muci vacuolated. eae necudOpodia Extended from all parts of tne periphery, ut ihe most prominent oné were from tae corners. These were Sometimes i Very lone, being almost equal in length to the length of the body. Q 1 menly . mo cGOntractile vacuole was observed. Leidy ('72) says that } They were oftei branched at the ends, and were extended and withdrawn the contractile vacuoles are numerous, but: that they are small and in- conspicuous. 3 ‘The thin ectosarc was thrown out around the animal and engulfed maé and everything with which it came in contact, so that the es were coreed with foreicn matier.The animal moved rapidty evenly, With a characteristic Sidewise motion. ani observed but two. This was on November 8, 1839, in material Mean aquarium Started a few weeks, before from Crystal Lake. Schewiakoff ('292) reports it from Australia and America. 5.Dinamoeba mirabilis heidy. Pl.vw Fig.29-I2- . iehdy ce to) pe eee. “rte! Vie VIL, 1-Il. is Miesanimal 1S Ovyate in form wien at rest,, but is very changeable. It is almost colorless except for contained food particles. The proto-| why a eq ral ow: - S 2eMspia 2 htegtaial 4121S ala nes PPh, GAGs dl MSE Ted aU ih ys SIRE TES pee ae eS actoiely 7 begaHy, fui ad its ie tye aah, rom! aie acd Ket eer peNeaae ae on agen os ys 45. plasm is sharply distinguished into ectosare and endosarc. It is granular and very mobile. The surface is covered with spicules which @o not Occur on the pseudopodia. Tae pseudopodia are uSually of clear. ectosarce and do not attain to any great length. They are extended and withdrawn very quickly. Both the nucleus and contractile vacuole were hidden because of the sorged condition of the endoplasn. Within the body were diatoms and many smaller food particles. One was Observed taking a diatom. [It was first caught in the ecto- Sere ajugd then in the: endoSarc, and .so passed in until it was all contained within tne inner, body. While swallowing tae diatom the body and thickening posteriorly, and then lengthening out again, until at last. it regained its normal state. One gorged specimen was seen exuding food particles from one side. This Species did not occur in great numbers at any time. One was obServed November 27, from an aquarium which had been about the laboratory for over a year and contained material from several Sources. Another was observed on January 5, 19C0O, and five more on January 29, 1900, from collections made under the ice in the Boneyard. Of these latter three were large ones and two were very 'small. One was observed apes Onnpril 2, 1900, from a Boneyard col- lection. schewiakoft ('eS) reports this species from America only. 6 Biomyxa yagaus Leidy. Plsske Fic. 62-25, Meld) foo eppeecel—257. Fl. XUVII;, fies. 5-12: XLVIII. ints is a larce, colorless, irrecularly Shaped creature. | It resembled a group of Amoebae connec ted by strands of protoplasm. | It was constantly changing, however, some parts growing together and others separating. At times the protoplasm would thin out So as iol Ye? ese Agieni’ ao ste Soo a te -* a * * 1 é Pie phAVan O8Se~ > tenis -. ie ’ A heer ahl vissliteetti tasltales - 2 S172 UBT RANGES wath surs to qed * ww 16GTy) 21307 G8 Serescon . oe neta foo. wh wlvoe 26 iqgtene eft eerk th) nee — = wy ~= pty 44. to form aoles. The pseudopodia were usually short and rather slender. They were sometimes branched at the ends and formed a network. The nucleus I did not observe. There were uSually two eood-sized contractile vacuoles but sometimes three. They varied in position with the change of siape of the animal. Many, Naviculae and Bacteria were eaten. Associated with it ee ee eon on oe oe FO ee ee et ee were Navicula, Spirillum, Spirocheata, Actinoparys sol_and Amoeba. I found, but one. This was on April 4, 1892, in material taken from aN aquarium started early in January, 1999, from tne Loneyard fear tie Neatine plant. It is reported and described. by Leidy ('79) from New Jersey and Pennsylvania, while Schewiakoff ('93) reports it also from Australia. Pa ‘Arcella vulgaris Earbe. Pl. 4. Fig. J6>S 7 emi Vien, 1f0-175. Pl. ZXVYI3) XXVIII, 1-7. Eutoewa s0- of) pp. 150. Pl. 11, fig. 2. This is a circular disc in shape, when seen from above, and like a concavoO-convex lens: from the side,. and is from a light to a very sore eorown in color. Jnere is’ a central spot which is lighter in color and which marks the place of the mouth opening. The shell is really dome-Shaped. This species of arcella_was not observed with sarcode extended. Leidy ('79) tells us that the pseudopodia are digitate and that the Sarcode mass is oblately spheroid. The specimens which I observed were of such a dark brown that both nuclei and contractile vacuoles were obscured. The animal did not creep about while observed. One edge of the shell is sometimes reflected back over the dome making the outline Of the shell appear more as a semicircle. The food consists of small diatoms and desmids ae we _ | La -@t SBan as aa RA ae gad afouda: act : ce 4 4 a “A ‘ic fe bP j It occurred in material collected from under the ice January 29, 1900, in the Boneyard, and which had been Standing in an aqua- rium in the laboratory. Schewiakoff ('93) reports it from As#a, Africa, Australia, Oceaniea, eee and America. Sey to) pp. 173-175. Pl. XXVIII, hes 4 a This species, too, is circular when spread out, but it is not SO thick as A. vulgaris. It is also a very dark, brown in color. The sarcode of this species was extended and was colorless, though more or less granular. The pseudopodia are long, dicitate, and sometimes branching. One granular nucleus was obser ved to the richt. It occurred associated wita A. vulgaris. in material collected from under tne ice in the Boneyard, January 25, 19CO. Schewiakoff © vo) reports it ras from Australia and America. . Difflugia _vlobulosa. Dujardin. PLAN Fig oo leidve 7Ieepp..26-98. FL XV, fies. 25-51; XVI, 1-24. pdS.1S spheroidal in shape. Usually the border of the shell is smooth, but occasionally there is a conical projection. They are light in color, some, being almost colortess. Many of the shells are ‘Made from quartz Sand particles but I found them in great numbers, / building shells from diatoms. Tne: mouth. is circular and subterminal. But one waS Observed with pSeudopodia extended. These were very delicate, pointed, and finally branching. | [tiOccurred during the Jatter part of December, 1892 and during January, 1900, in an aquarium of several months standine. Schewiakotf ('95) reports it from Asia, Australia, and America. Butschli ("80~'82} gi reports it from Europe. fos O) 10 Difflugia pyriformis ferty. PL ati Fig. 10-42. Deere 70) pp. eo-1os. Pl, ar 41: XII, 1-18: XV, 32-53: oi, oO; XTX, 24-26. The shell is flask-shaped, with a neck-like prolongation at the narrower end. The shell is uSually brownish in color and is made up Of angular bits of quartz sand and some diatoms. It present*a: bi- lateral Ssymmetry,.i.e., a-line drawn, bisecting the mouth and perpen- @icular to the plane of the mouth, bisects also the fundus and divides the shell into two symmetrical halves. The mouth is terminal. The animal puts out very long pSeudopodia which move slowly. They are round at the end and branched. The protoplasm is usually Granular. dhe pSeudopodia extend far out and procure the fod, which consists largely of diatoms and algae. It also moves by means Of tne pseudopodia. several contractile vacuoles were observed, but the nucleus was not seen. One waS Observed dividing. The sarcode divides by cross division and the naked animal formed provides 2 new shell for itself. The Old shell was quite dark, while the new one was colorless. The two mere we Same Size, which Seems to prove that the shell does not erow after its formation at the time of division, a conclusion which enard ( elas receduly réatiarmed. | first found this Species in uné, 1899, since when it has occulred.in nearly every collection made and in aquaria about the laboratory. It was the largest Difflugia ee © Se ee largest species known. Scarewiakoff (193) reports it from Asia, frica, @ustralia, Oceanica and America, and Butschli. ('80-'S9) | from Europe. ‘lek : wot a iG sci onus at ata Z +‘ JO Sa x at 20> lea 1 + 74 ih ehoepyns D i210 ase i [oe -ean eee 7. cw hve testen Pyer Hho gw RY ; | Jatt Sens J orts o2B2 a! , (OES SERA ele eet 7h ot -aad , . ji atic \wEkb 2 Wewkthiesy clreeces Ge ¥ « ™ ard iised wh betlossd kc /) ode sont ee $i fs | OP See 7 -YIereiof et) aes hubds- shige ee tem) soteis 1ST‘: vated faneotie , eee GO. oa rt ringer here. is..a clearer space im. taccecnter.er,. the. body.,.waich :»:roh- Bory represents the location of the nucleus. The contractile vacuole is sinele, although it did not always reappear at the same place. It pulsates very slowly. The animal moves slowly. At first one would think it was fixed, , because of its easy floating: movement. These were found rather abuudantly in a collection made Jan- vary 5, 120C, from under the ice. It is reported by Schaudinn ('9¢) from different parts of Europe, North America, and the East Indies. oe —— Lm feos fer at amen att, eae ar hale Kent ('S0482) pp. 221-222. Pl. I, fig. 30. This was found first October 25, 1899, in a collection made from| 23. Mastigamoeba siamlex Saville-kent. Pl.x/4Fig.52-55 e | | | Sound +t Since , rather abundantly in aquaria about the laboratory but P not in fresh collections. It is reported from Ireland and from Germany l ty ti -This is a small mass of protoplasm, which is amoeboid, i.e., | constantly caanging in Shape. It is very small and almost spherical | | when contracted, and is differentiated into ectosarc and endosarc. | fit has both pseudopodia and a flagellum. / ! The pseudopodia are usually but two or three in number, and | are generally directed backwards. They are exteiied and withdrawn jouickly and somewhat reeularly, i.e., first:on one side and then on ‘the other. There is one flagellum, alittle longer than the vody. mere agirected forwards and is in constant motion. Taere is one contractile vacuole situated anteriorty, but tae hucleus was not observed. Teiound tais species twice, once in April, 1399, and again in Marca, 1900. cota times, it occurred in aquaria of lone Standing eecsoues not foul. It is reported only, by Kent ('SO-'&2). who worked ! in Englaad. | 24. ‘Cercomonas ‘typica pavidde-Kenthy Fl. AX, fic 56,09, re me ee re me ree ts me pet me ee ee ee ne ce | } Ment ('S0-"82)\p. 259. Pl. XIV} figs. 22-30. Butschli ('80134) enol, rie. 12" ia¢e is 4 stall colorless form, spherical when atrrest,, but soft and changeable in shape wien moving. The posterior end is drawn Out into a thread-like process. OL Thereis one flagellum, four five times as lone as the body ave diameter. It is very active, lashine in: food particles and assisting in Swimming. There is one contractile vacuole near the center of thebody. | I observed one in the process of division, which is a simple act | | of cross-division. After the two have nearly Separated, they are come the posterior ro eees put this finally divides in the middle a ‘they (seit apart. | oF | connected for some time by the protoplasmic thread which is to be- oO. | _ This was found throughout tne sumer ane fall of 1899 i) | > in lavor- jatory aquaria. It is also reported from infusions , by Kent ('80-89) 25. Stylobryon abbotti Stokes. Pl .Yxi,Fie. 60, ae pee dhe aeee sees ome prores’ ('SSB pp.. 79-81. Pl. eres Le. The lorica is conical in snape and is about twice as long as broad. Ine enclosed body is small and ovate and is attached to the posterior part of the lorica, but does not half fill the lorica. | They form colonies, two crowine out from each precedine one. I did mot find a colony containing over seven individuals. They apvear to be Sessile on the antero-lateral margin of the supporting lorica but are really attached to the iuner lateral wall by 2a short stalic he primary stalk is about six times as fone: as a lorica’. The enclosed, body has two flagella, one short one and one long ome. The body darts forward and projects from the lorica and then, it disturbed, retreats into the lorica. laere is one contractile -vacuole posteriorly located. These were found in April, 1900, in aquaria, attached to threads 26. Paramonas slobosa Promentel. “Fi. -Fie. foie 60 — S20upe oie rl. AA, fig. I. This has a small Spherical, body, is very granular, and quite persistent in snape although slightly distorted when eating. The Peath is large and circular, Situated at the base of the flagellun. There is one long, actively vibrating flagellum, which lashes in ood bodies and transfers them to the mouth openine at its. base. The contractile vacuole is simple and is Situated near the peri- phery. They occurred-abundantly durine Septemver, October and November, LIT TOGSee 3 7 Z 7 . abi b _USTMONge..8 a Ol ~ in aquaria which had been standing in the laboratory for. some time, | It is reported from furope. | <7 Anthopaysa vegetans Muler. Pl Aa i. 6/-63, ae So- see pe eov—-71. Pl.- XVII, figs. 13-26; Tite l-lO.putcsenli GS0-"69) Pl. xL1, fig. 5. The body is irregularly pyriform and is truncate anteriorly. ‘| The zooids are erouped in rosette-like clusters at ine ends of gran- | ular, brown, branching pedicles, which are arranged in branching colonies. There are two flagella attached at the anterior end and both eeewaoerected fOrward. One is shorter than the other. Miesnucleus iS situated a little. below the center. There are two contractile vacuoles in the posterior part.. Sometimes the clusters or heads break loose from the pedicle and then they go Swimming about rapidly and with a whirling motion. sOmetimes, too, the clusters break up into Single zooids. IT found these abundantly-—in June, 1829, in material from Crystal Bake. In March, 19CC, they. became very abundant in an aquarium, forming a thick brown Scum over the top. Iney are reported from Europe, ASia, Africa, AuStralia, Oceanica, and America by Schewiakoff | (195). 28. Euglena viridis. Ehrbg. Pl. auFig. 64-46. Tei Geleppe ocl-ose. Pl. XX, fies. 29-51. Butschli sveveomerts AyViL, fice. €. Tiis is a highly metabolic animal, which is sub-cylindrical when at rest, with a short, pointed, tail-like profongattion. It.is full of ovate chlorophyll bodies which give it a green color. There is a red pigment sPot at the anterior eid. Ihe surface shows a faint oblique striation. Tne caudal prolongation is colorless. The flagellum is Sinele and is long and slender ae | | close to the red pigment spot. and Europe. /body is ereen. Tne surface has oblique rows of bead-like »; = places. a The nucleus is spherical and is centrally located. The contractile vacuole is located at the anterior extremity These were found at all seasons, throughout the year, in aqua- Seeeeand if t2€]0 collections, even under the ice. It is reported by schewiakoff ('95) from Asia, Africa, Australia, Oceanie2, America, <2. Euglena spirogyra Ehrbe. = P1.AX¥Fig.67. Sie coe. Fl. KX, fies. 27-28. Butschli ("80-'88) Pl. XGVII, fig. 9. This is an. elongate animal, six times as long as broad when ex ° menged. [£18 slichtly truncate anteriorly, and at the posterior end #S prolonged into a decided *tail, which is colorless, while the There are two Oval amylaceous bodies located about one-third of the way trom the head and from the posterior end of the colored body. There iS an anterior red pigment spot. - . . | The single long flagellum is directed forward and is very active, The nucleus is spheroidal and is located about the center, be- tween the two amylaceous bodies. The contractile vacuole is anterior and is in close relation to the pigment spot. | These have occurred throughout the year in connection with St mewieamnanwneseseme i -ceenencncoans E. viridis and —. acus, but not so abundantly as *0°8®species. schewiakoff('93) reports it from Asia, Australia, Oceanica, America, and Europe. 30 Guglena oxyuris Schmarda. Pl.AxvyFig. 6s. Poe Oe pie O80. FEC XX, figs 26. This is an elongate form which never straightens itself out _ - Qi is 6) put is twisted spiraly. The anterior end is rounded, while tie nost- | erior end is prolonged into a curved, abruptly and snarply pointed | tail. Pe iS ereen in color and contains two elongate rectancular ") amylaceous corpuscles. The usual red pigment spot is developed in ) | the anterior extremity. Ihe surface is obliquely striate. The slender flagellum is about equal to the body in length. The nucleus is oval and is. located centrally, between the two | rectancular amylaceous corpuscles. The contractile vacuole is large and is located just at the side Of the pigment spot. I found this form but once. It was in association with the other re me of Crysial Lake the latter part of September, 1899. Schewiakoff ("23) | reports it only from America, but Kent ('80-'82), who worked in England has also reported it. / ol Buglena_acus Eorbe. PL. XXWAFIiC. G67. Pend (S0='S2) pp. 385-084. Pls, XxX, figs. 24-25. Batschli meO="89).Pl: XLVII, fie. §. is ‘ Body is elongate and very slender. It’ten times as long as =) broad and tapers toward both extremities, Tie anterior end being abruptly truncate, wiaile the posterior one tapers to a point. The body is green in color and contains many elongate rectangular amy laceous bodies. A red pigment spot is developed at the anterior end. The flacellum is Slender and is not longer than the body. The contractile vacuole is larece and is. situated in the anterior -end just, back of the pigment spot. ee ee ee ee ee Uceanica, and America, while butschli ('S0-'89) reports it from Burope. : . a : = Ay . \ i 4 * ty < 4 r : ~welhon & ~ « T A 2 ana ts 23) Fidhatence. opps ngs thoes iy bene cane puce ® fax rt ut Sthoter aif? set one = mn maa ines cee aw Becy > a 's 2) suldael, itech oem ‘ ry 7 . : ih Bevo al 2yaleonneee #l i ee a — a + Al | « . Pe he [ ;, P “) S== ze 2 = \ —_ _ s , oth") . ale of yf : FS pil te feels ohare 9d elias | bin igo Gf eaeteral mal bas | ’ . wit Tatoaty at mm lieeal? +9 inept an) 16 sont im a a5) Oo ve. Ipacnelomonas hispida Perty. = P1.XXWAFig. 76. ment ©°S60-"S2) p: 590. Pls XXT) figs. 21-23. Butschli- ('&0- gee FS AVI | fies 2. These animals are provided with an evenly ovate,dark brown shell, , ‘which is hispid upon the surface which also has a scarlet or crimson | tint. At the anterior end is a very short cylindrical neck. The | i" inner body is granular and vacuolated, and fills the snell save for } a narrow border. There is a red pigment spot in the anterior end. From the neck extends one lone Slender flagellum. There is one contractile vacuole situated near to the pigment | Spot. | This species was Obtained durine the latter part of September, eeeinea tow net collection from Crystal Lake. It is’ very cosmo-, politan, being reported by Schewiakoff ('93) from Europe, Bere Aus- ‘tralia, Oceanica, and North america. ve Iracelomonas acuminata Schmarda. Pl. Fig. Peete soe pp etS91s4P1l kel), fig. 26. | Thesiell is flask-shaped beine inflated posteriorly and with the /pasterior extremity produced into acuminate tail-like process. inesanterior end is. produced into a short, obliquely. truncate, cylindrical neck. The inner body follows the shape of the shell, and | mearly fills it. A red pigment spot is located in the anterior part of the granular inner body. The lone slender flagellum protrudes from the short neck. | The contractile vacuote is located in the anterior etd close to the pigment spot. _ I found but one specimen of this species. It was associated with two. aispida in tow net collections from Crystal Lake , September 30, 1892. It is reported from Europe. | ee. ae t OT 2 KYRA 61. 34.Phacus ‘triqueter Enpbes PF). kXviFic. 7/. On Gy OF re oe ow =m Ow On On adh He ome See oe em oO Se) pp. se7 i Plage fie. 1. This is a flattened leaf-like form, with a ridge down the center Of the right hand Side, with a pointed tail-like prolongation. dae mouth is terminal. lhe surface is longitudinally striate. | Itis ereen in color with a red pigment spot at the anterior end. | There is a siugle lone flacellum, arising from the mouth. |. The contractile vacuole is small, and located near to the “7 mp@ueam the anterior end. ESS SE penewiakoff ('935) reports it from Australia, Oceanica, pee and | Europe. On. eens oneicaudus Ehrbe. eaee Ph, Pie isoaige) pp. 367. Pl. x41. figs. 647. Butsehli, ('S0= 189) Pl. XLVII, fig. 12.. The body is flatténed and leaf like in Shape. It is green and | Contains a red pigment spot.in the anterior end. The surface is lone- itudinally striate. Tne body is usually twisted more or less on its axis, and has a caudal proloncatio which is long and pointed, and which is equal to the body in length. 2. _longicaudus does not ‘have the ridge down the right hand side, is’ more flattened, and has a much longer caudal prolongation than, P. “trioueter. The flagellum islong and slender. The contractile vacuole is located in close relation to the nioment spot. I observed this ‘Species, but once, September SO" age. Lt was in association with P. tricueter, Euglena, Hallomohas, and green | ‘ fee bay garters alent eta oe Or ee mE ee nm ee nee tee nme algae. The collection was made from water bloom on tne surface of Crystal Lake on a sunny day, september 25, 1892. Schewiakoff ('935) reports it from Asia, America, and Zurope. ee , S “ : i > a s ¥ ‘ y ' oo aa a ¥ > Boats fet . ; = -_ ‘. = f * » -@ cy = ge wi tle KOtveipoeeee > “~~ ’ a@tisa tics on 5 : ; ts i a i 62. > waa rier aa XXIX, ae: Petal none mediocanellata Stein. Fly. feos ves S ceemedkeneimealiondieelieaeientioe dd Lee me re eee — Rent ('80~'82) p. 371.. Pl. XE, fic. 3. The body of this animal is ovate, rounded posteriorly and pointed | anteriorly. The mouth is terminal and. extending, back from it in a | | median line and almost to the posterior end of the body, is a groove | @eecnannel: It is colorless. Tnere is One yvibratile flagellum about equal to the body in sleneth and which is very active, drawing in food bodies with which eeecomes. in contact. The contractile vacuole is situated in the anterior end, to the left of the median groove. The nucleus igs more posterior than the contractile vacuole and is On the right hand side of the channel. They multiply by longitudinal division. This process was obser ved by me, and while dividing, the flagellum was very active in each. | Proud: -i t in October, 1899, in aquaria in the laboratory: schewiakoff ('93) reports it from America, and it has also been found a Europe. o7 aStaSia irichophora “hrbg. pra ate. Weme © o0—" Ge) pe aro. Pl. 2e> fies. 17-214 72. Maen Stretched out, the body, which is a colorless: mass of protdé- \r plasm, iS about six times as long as broad, broad at the posterior €nd and tapering sradually at the anterior eid. The protoplasm is homogeneous, containing many granules and vacuoles,a distinct ‘pharyngeal cavity, and a nucleus. It is extremely mobile and contorts itself into unrecognizable shapes. | | | The flacellum is fixed and is about one and one-half times as long as the body. It wraps about food and draws it into the mouth, whica is terminal, and which is succeeded posteriorly by a pharynceal i : i j c - ly c A, P | « : ‘ yt as PROUT a: pas: ol : - ‘(at : a | a : a) * 7 ; « i ¢ G a © ea 4 mae ; ; ‘ _ 7 Oo ets las oie a beaten ' Aa e r% ie) | ) nt Of TT , n ; Th oy a Loos 3 tive ang ¥ J ; ‘ af a . i : 5 | 7 ~. a8 | i {. ? ¥ é 7 y ' \ F’ ; £ shy : } ’ f od L 7" ‘. Pleodorina illinoisensis Kofoid Pl. Fig. Kofoid ('98) pp. 273-293. Pl. XXXVI; xxyvIl. his is an ellipsoidal eolony of S2, rarely 1c, cells and is ‘a ons tant. in shape. The cells are of two kinds, vegetative and li tec n zonidial. ‘The vecetative cells are four in number and are in the as interior end, peine always directed ahead. The cells are zreen.in witn a reddish brown stioma in the anterior end of eac. a ) Prat nc el] nas a distinct cell membrane . B mach | cell has two equal flagella, which unite with the cell cae a ee anterior end, adjacent #6 the stiema. - The mucleus lies in about the center of the cell in the midst | of a mass of protoplasm enclosed. by the chromatophore. No contractile vacuole was observed. The colonies moved by rotation on the principal axis, some times Pight to left and sometimes from left to rent. aay occurred abundantly in tow net collections from Crystal Lak@, Kofoid im 159a, (* 1899. This species was described by br. v. i. weve 46. Platydorina caudata Kofoid. Pl NAXLFic. 77 Sy res eee See Sieg oe ee Se ee re ee eee ee he oe "ofoid (19°) po. 419-440. Pl. XXVIII. This is a flattened, norse-shoe-shaped colony of 1€ or 32 ane cells. There are tiree or five tails formed at the posterior wy the extension of the common outer sheath. The colony is some- times twisted about one-eignta of a turn from right to left. here s » is a a ae san in each zooid in the protoplasm at the anterior - s ae 7 ‘ " “-s . pep ena Liethr ching G2 ee a 7 - = ay alias alt i Pi : : ae oa 2.21 Oe Me rerio ane . : rg is " ; 7 8 mit | & : iy 7 . . : 3 : = a et ier! Lk @ ae xis ; nie ‘eect B/ ‘am eS rabbi et nf i- fy La I : ang) a ae taf i” We 2 <3 ee | | york % - : bites) abe) Nery riper eee a. bee " yeh tin! ei Lindh wre a ais tA : og he fy o oo Wea» 5d | There is a prominent central nucleus, round in shape, and con- tainine a central round nucleolus. There is one small contractile vacuole situated a little poste- rior to the pigment spot. I found this Species abundantly in August, 1&9, and less abund- analy in september, 1692. It was_reported, and described by Kofoid ('92) Soreine first time. It has never been reported outside of the Mississ-z ippi basin. | 47. Volvox aureus Ehr bg. Bae) Sue. Monona (SS) pp. 427-426. This is a colony of great numbers of cells, .arranged in a Sphere or an ellipsoid. the cells are all connected ty protoplasmic proe- cesses.into which the chromatoph*re does not enter. The animal moves by rotation Oon_its principal axis from left to right, which rotation ay be reversed occasionally.*Eackward motion is rarely seen and asts but a short time. This Species was abundant in tow net collections from Crystal bake, August 5, 1699. It. is repor ted from Europe and America. 48.,Feriginium taqulatm Enrbg. Fl. Fig. | Pate CEG. VEe op. 446-420. PF] ., X80, fies. 1+£ and - ff <£7. Peiteenla { ECZEQ) Pl. LIL, fig.66. The shell is a somewhat elongate ege-shape..the, body is divided transversely into two almost. equal parts, by 2 ciliated esroove, and the upper half is divided longitudinally by another eroove | which is not ciliated, while the whole shell is irresularly crooved, Mlarking it off into polygonal spaces. .The mouth is on the ventral Side at the junction of the transverse and longitudinal erooves. } ne shell is of a brownish hue and has a redpigment spot developed. | .thnere.is 2 single long flagellum, which arises from the oral | aperture. fj o—_— 69. c found them eee in September, 1899, associated ted by ee ieiorrt: 93) from Asia, Australia, Oceanica, America, and Europe. 49. Ceratium kumaonense Carter. Pl. XXXU,Fige FO. ee ee ee ee re ee re ee ee ee ee ee ee ee Kent ("80-'82) Pps 4oo.F). KXV, fig. 25. The shell is triangular, having two anterior and one posteri- or, large horn-like processes. These processes are produced from the angles of the triangle. The shorter anterior horn and the posterior one together are a little more than equal to the body in length. The other anterior horn is about half as long as the other two. They are all finely cerrate. The shell is divided trans- versely into two equal parts, by ciliated grooves. The shell is brown in color. The mouth is located about the center of the body. From the mouth arises a single, long, slender flagellum, which is very active. They were very abundant in a tow net collection made from Crystal Lake in September, 1899. They were reported in 1871 from Hindostan by Carter ('71), and this seems to be the first report | of their occurrence elsewhere. | 50. Enchelys’ SD. Pil Fig. ee me ree ee ee me This is a colorless, elongate, cvlindrical form, with the ante- rior end somewhat pointed. It closely resembles Enchelys pupa, but differs in that it is longer and more slender. It is very | contractile, and its movements are manifold. The body is delicately striate longitudinally. The mouth occupies the anterior end and | there is no pharynx. ! The ates surface is evenly ciliate, but about the mouth the | == 70. ilia are longer and are very thickly placed. The nucleus and contractile vacuole were not observed. They occurred but once and then very abundantly for a few days during the latter part of March, 1900, in aquarium started from the Boneyard. ~~ en 51. Coleps hirtus Ehrbe. PL. XXMUWFig. SL BZ. Kent ('80-'82) pp. 506-507. Pl. XXVII, figs. 3-4. Butschli ('80-'89) PI.LVIII, fig. 1. . The body is barrel-shaped,rounding posteriorly and about ‘ twice as long as broad. The cuticular surface is divided into ‘Many square spaces by longitudinal and transverse ridges. The most of the specimens observed were brownish in eolem The cilia are found not on the squares, but in the grooves between, and are very active. The animal swims rapidly, revolving on its longi- tudinal axis. The mouth is terminal, and is surrounded witi cilia of a larger size than those of the general surface. There is but one contractile vacuole, which is placed poste- riorly and can not always be seen. When this species was found it was usually in large numbers. It occurred in the aguaria at all seasons of the year and was especially plentiful in very stagnant water. It is reported by Schewiakoff ('93) from Asia, Africa, Australia, Oceanica, America, and Europe. 52. Amphileptus Yanser Ehrbg. PL.XxXANM Fig. %3. ~~ eee ee ee ee es Rent {"30-"82) pp. 526. Pl. XXVIII. figs. 39-40. The body is elonsate-lanceolate, pointed posteriorly, and with a neck-like prolongation in front, equal to the body in lene th. ‘ i] The protoplasm is very granular and very vacuclated. The animal is a ¥ ‘ The is - ™y Aan ; pam <4 5 wi Vier. 4 710q : «Faring? age 7 : 7 / a : i i 1s ed bei .. ar * “ U : . a4 aT otto | Sinai 102 | ‘sootounas hte. ‘aoe ued woman > a > 7 am. i ft40% ‘Tetat , i eo é 1 | fhed ut ‘ , , ¥ re ‘ rd 5 “e° bag KY eqn aft? wet sitaupe spt at ber | 107m lulitmele vil led ot? (8R") Vieee 1s ; Q f Ce) yao tls | ‘| very flexible. The mouth is situated at the base of the neck. | The whole surface is evenly and finely ciliate but the cilia around the mouth are some larger. The aeronucleus is bi-lobed and lies near the center of the body- | The contractile vacuole is garse and situated vosteriorly. These were found in abundance in collections made from under th ice in January, 19000; also in early March collections, and they have continued abundant in aquaria founded from these collections. It is reported from Europe. ee 5S. Lionotus fasciola Ehrbc. Pik. Fig. Kent ('80-'82) op. 743-744. Pl. XLII, figs. 5-11. Paveenl 1 ("802"89) Pl. LIX, fig. 6. This is a colorless elongate form. The neck, body, and tail are not so sharply distinguished from each other as in Le wrzespi- owskii, The neck is less than half as lone as the body, and is not. so slender as in the other species found. It is set with tricho- cysts along the left border and the cilaa are larger than those | of the body. The tail is short, and pointed. The mouth is situated at the left and about the center of the middle vortion. The ani- | mil swims rapidly. It is very flexible but not contractile. The single large contractile vacuole is situated posteriorly. This was observed in February and in March, 1900, from mate- rial collected from the Boneyard on two cold days. This material was collected in February and remained stanHine in the laboratory. Schewiakoff ('93) reports it from eee Oi nt ia. (Oceandicas Americas and Rurope. ry ee Pe tk f4¢ Ad, oA Co y icin yim ve sad “onl | ‘Tetial Mie. rete gi ‘ so eer aaa soe ich eee oT | ‘ + oftdonv va ‘i . ont ‘ i Gongs i- Toe ~€ My . ae Uimcinalys | | « heunhtd i} 702 x [th vl Qrene se town onl @ Vedio off #f =e qaeba wiod +tal ef wrote a (Sante . (ies ast athe “so ent Jdgeds itis etel: ise at eb gir see st ines vgtal etpate ef Alvona y fet ae Nevregde eae “eu . ; ‘one OD Oe. a : ‘ od 22 Sort berustt ‘fii-ah wl tina se Renliee: Ane eran det ah anoQ + ei Tee saes eer | # ) straddle, ; nd = a T2. 'Y, Pic. Su | XXX 54. Lionotus wrzesniowskiiSaville-Kent. Pl. I Mant © "60." 82) pp. 742-745. Pl. XLII, figs. 12-13. Butschli ('80-'89) Pl. LIX, fie. 5. This is a colorless, elongate form. It shows three distinct regions. The anterior portion is prolonged into a slender neck which is very flexible and is about half the length of the body. “The cilia in this region are longer than those of the rest of the body and there is a row of trichocysts on the left margin. The mouth is at the base of this neck, to the left. The central thick~ ened portion contains a granular endoplasm. The cilia are fine and ‘evenly distributed in this region. The posterior vortion is a short, Clear, pointed, tail-—like region. There is mn bi- lobed spherical macronucleus about the center, and alarge spherical contractile vacuole at the vosterior end of the thickened vortion. The animal swims very rapidly, bending and turning particular- ly in the neck region. It is very flexible and contractile. This was first observed in December, 1899, in ag aquarium Started in the fall from the Boneyard. They occurred here in num- bers. Again in January, 1900, I broke iee near the bank of the Boneyard and found many of them. I have not observed them since. | 1} Tt is reported from Europe by Butschli ('80-'89). . i == - NON URV | 55. Nassula_rubens Claparede & lachmann. pi Fig. 53 Kent ('80-'82) vp. 495-496. The body is ovate and is ecually rounded at both ends. It is finely and evenly ciliate throughout and contains a gran- ular endoplasm. The mouth is situated one-fourth of the way back | on the left hand side, and leads into a pharynx armed with Beyl- is oft iw lead be ‘aA oo tyeezgw E> GhT, -. a0 (oa Gaal eT = ~ A, a8 1 nae eae io ee he * shred és ca 1d +o ii.shl yews iia ot) A mt i. al Stas ’ 1 aunel: comm hawet tax beg i Age iu@eud. sdot wz WoT? hertoqer ‘isceh gqegyr sluers 8 Ok “sam (86-089 dines | ete Pe sed sah inte f bod einen lee taat tum t evesi cut wert? ¢ ‘Ss Aru | fans bs wes) mies | a ad ea aS potest | 4 h¢reag ie nin? ae [eas Pe 13. i” € dilated anteriorly. The most striking characteristic of this species | | is its striking and brilliant none color. | | There is but one macronucleus, which is large, granular, and is located a little back and_to_the right of the : center. The contractile vacuole is single. It is large and spherical, land is situated_to_the left of the nucleus. It sometimes leaves _two smaller ones after systole. ee ee } observed_two of _these on March 20, 1900, in an aaouarium which had been started about two months before from_the Boneyard and had become foul. A scum was formed on.the top which was largely made up of Stentors. It is reported from Europe. by Ms Oe 56 Chilodon cucullulus O.F.Muller. Pl Fig eee ee ee meee em ee te ee ee nee EW ore oe Ment ('80-"82). pp. 746-747... Pl... XLII... figs. 16-22. Pubeehld <'60-'69)- Bl. LX». figs, 8. The body is flattened, sub-ovate in outline,. and is very flexible. It is rounded at the posterior end: anteriorly, on the right side, the body is projected forward in a sort of lip, and meeecurved over to the left, The cilia about this projection and down the left border are larger than elsewhere. The surface is longitudinally striate. 4A vibrating line leads from the tip of the lip into the mouth opening. This line projects a little beyond the | edge of the body. , | The macronucleus is single, ovate; and centrally placed. There are many contractile vacuoles scattered irregularly throuch the endoplasm. } This is one of the first forms which |] observed. It has oc- a 6 ad L «Be eee 1, Loe oe ;° 726 He sleit oleae tin a ; °F " b ards i? F a oe ae an he iA ‘Liigd She eripeee rl Pa: és *2 7 ' 7 7 , ¥ : eo eer ~y itr) tid z m5 om ‘ as 2 ; + near » - } : i hed br r 4t ” ar 7 ve | ’ : o { C ; he r . - . bl 14. uurred plentifully throuchout the year, and especially in very ee ee ne ee ee ee eee ee ee | Asia, Oceanica, America, Europe, 57. Glaucoma scintillans Fhrbg. Pl. Fig. 0 ew ee ee ee ee ee ee ee ee ee mm ee aad Kent ('80-'82) po. 795-796. Pl. XLV, figs. 39-40. Peer 0-89) Pl. LEXI. fig. °S. The animal is egg-shaped, being rounded posteriorly and a little pointed anteriorly. It is somewhat flattened. The animal is colorless. [It has a steady and rapid movement. The body is long— itudinally striate, and is finely and evenly ciliate. The mouth jew on the ventral side, toward the anterior end, a little to the right of the center. It is curved and is bounded by a strong undu- | lating membrane. | The large spherical macronucleus is contractile. The contractile vacuole is single and located posteriorly. This was found in a mid-winter collection from the Boneyard’, in the latter part of January, 1900. I saw but a single specimen. | Schewiakoff('93) reports it from Africa, Oceanica, America and ! Europe. | ; | 58. Frontonia acuminata Ehrbe. Pl. Fic. Biteen id’ (F080) Pla LXII, fig.’ 4. This has an ovate body with one end rounded, and the other prolonged into a point. It is somewhat Pip ichade, Theictnt ate is longitudinally striate, and is finely and evenly ciliate. The elongated mouth lies along the left side on the ventral surface, | and has an undulating membrane. The endoplasm is considerably vac- olated. The nucleus is oval and centrally situated. oa 3s te ate ae es ' z ba a Pipa at . ; veanTUS a cathe "ere ridden alien 7. = [ — ; a? ey 4A 88 oor nn vty tr rege 8a (3@ i oo le 349 , ‘ # = 7) oaxys nes na un) saw e - lo ogra ne ; , sha a wo : ME he 3 tio “ tn oe a TB the re is but one contractile vacuole and it is posteriorly t E ted e 1 found this form but once. It was associated with Glaucoma — — ay scintillaps. It is reported from Europe by Butschli ('80-'89) . 59. Colvidium colpoda Schrank. PL.AMVYFic. SZ SN ee rE ee ee eee re Meese? «e0-"S9) Pl. LXII, fig. & The body is kidney-shaped with the anterior end less rounded | than the posterior. The endoplasm is considerably vacuolated and 1 fe | | granular. The surface is finely and evenly ciliate and is longi- ai] — striate. The mouth is on the ventral side to the left, ry ) . and is similar to that of Glaucoma. It is curved and bounded on the | sides by an undulating membrane. I The nucleus is oval and is centrally’ located. } ; | The single contractile vacuole is posterior. - “first identified it March 5, 1900. They were very numerous Eat that time. It occurs abundantly in aouaria which are stagnant | anc contain numbers of Paramoecium and Bacteria. Schewiakof f (192) | reports it from Africa, Oceanica, America, and Europe. be i } Loy 60. Paramoecium caudatum Ehrbe. piss ie. TS, FF en ee bee ee me ee ee ee ee re ee ee ee me ee ee Piagetian e0s, 69) Fl. EXIT, fig. ]..Blochmann ('95) py JOS. Pl. VI, fig. 194. This is an elongate spindle-shaped animal three or four times as long as Lohan The posterior end is somewhat pointed, and there the cilia, which are evenly distributed over the rest of the sur- Rea: are longer. The mouth is at the termination of the oral | groove, which extends from the anterior left hand extremity peciiee riorly over the ventral surface, to the center of the body. The | animal has a complete coat of trichocysts. % qt 5 Vhit ie 1) . 7 » - + a Tus PS “ - ” gli aaa <') aren bh Pot ee wood sotreteog! a nirpe ant tah 4 “taivia£ 7 ot veltet | if ae ion Oo ae ot (oo wine ll eek tae - ‘1 vente ‘ ; Joni & ‘eit mor se Tete 2S Lidaesut 'V 919 SOT -@ rsvrote We ms al 16. “The macronucleus is large and ellipsoidal, with one micro- ucleus. They are situated about the center of the body. | There are two contractile vacuoles, one near the anterior, and: f] one near the posterior, ends. They freouently have a star-like | appearance, due to the canals leading to then. q I have frequently seen them in conjugations, with the oral groove closely applied. They occurred abundantly throuchout the year, especially in stagnant aquaria. Schewiakoff ('93) reports ther from Africa, Oceanica, and Europe. | 61. Brocentrum turbo 0.F.Muller. Pl. Fie. Kent ('80-'82) pp. 641-643. Pl. XXXIII, fig. ep Pratachli, (180269). Pl. LXLX, fic», 15. The body is somewhat pear-shaped, being largest at the vest-— | | erior end. There is a stylate caudal appendage which is about | | bis the length of the body. The cilia are distributed in two | wreaths which encircle the body. The anterior wreath is just a | littleback of the anterior border of the body: the posterior. | | wreath is a little back of the center of the body. The mouth lies | on the ventral side in this posterior wreath of cilia. The animal G swims rapidly with a whirling motion. | Both the nucleus and the contractile vacuole are located poste- | riorly. The vacuole presents some peculiarities, by takine ona | series of forms. When it is full it is. round, but on contracting | it has somewhat the appearance of a rosette. The nucleus is band- | like. -[t occurred in November, 189, in.a large aguarium started | ‘more than a year before and to which had been added, besides Bone- | _ yard Material a little water from the Illinois River at Havana. | _Schewiakoff(' 93) reports it from Asia, Kustralia, geome oe Ate ee ) sioueas ed? ad .earro) to me P en! erie Oe Cat vip! w wll 2 - + oy = | TV bi AXAVIIhy 62. Pleuronema chrysalis ‘Ehrbeg. ag Fig. 70. Per ©0=' be) 'p. S450 Pl. XeVIT, fig. 55. Butschli | fee OO) Pls LXIV, fig. 6. ° Me ‘The body is ovoid, eoually rounded at both ends, concave elo and convex above. The cilia are somewhat rigid and ane: deuete ! @ all over the cuticular surface. It is rather ouick, but when | irritated, it moves out of the way with a ouick leap or spring. A large extensile undulating membrane is found beneath ventrally tached to the left edge, which may be either ektended or ne. | drawn. The mouth is located centrally, on the ventral side, in | a littlecdevression, and leads into a tubular pharynx. i | The nucleus is situated a little below the center. | The contractile vacuole is single and is located toward the | | posterior end. | | mtr Food is caught by the extensile membrane. [| have often found | | the animal paired, as thouch in conjucation. | | a They occur very commonly and in great numbers. [| have found then| _throuchout the year in various aauaria about the laboratory, | often with Paramoecium, but more generally present than this genus. | | Schewiakoff ("93) reports it from Asia, Africa, Australia, Oceanica, | “America, and Europe. | . RAIN 2 63. Cyclidium glaucoma Ehrbe. Pl. Fig. D/ ee ee ret ee Se re ee re ee ee er oe Kent ('80-'82) op. 544-545. Pl. XXVII, figs. 57-58. Butschli ('80-'89) Pl. XIV, fac. &. | The body is ovate, convex above and a little concave beneath. Fine setae are developed over the surface, and at the posterior 44 | _ end are several very much longer setae. The mouth opening occurs NTA Ane SAOEEER yensyiyu) - ; SAA = hi i W4 EEA af ‘ ce f a I8 ~~ “ i ’ Vee A ‘ui < 7 ’ er2 Pay re *) SET act P = ~~ —S —_— sag roqeT: (S@") |} lot 7 4 " my _ ; eqoTticg bre aa “TSE RRSE : t | | ova) eo ys ot : ; : ace: * a : ‘ on i ’ ~— + % | idsetua a Rociaee bas deve ate rod. aft . . A eres ene. Sa ae 78. times extended. 7 a : The nucleus is spheroidal and is situated below the center. The single contractile vacuole is located in the nosterior end of the body. joe These, too, are very common in all collections throughout They are reported from Asia, Africa, Australia, Oceanica, America, | and Europe, by Schewiakoff (19%). 64. Spirostomum ambiguum Ehrbe. Pl. Fig. Se es eo er ee Kent ('80-'82) po. 586-587. Pl. ¥XI¥, figs. 13-14. Batechii iC"S0."80)' Pl. LYVIT; fie. 2. The body is an elongate cylinder, about fourteen times as | | a | long as broad. The animal is colorless, flexible and contractile. Tre | 4 i} | | | mouth is an elongate opening beginnine in the middle of the ventral | Bids; and extending down into the middle of the body. The whole | cuticular surface is finely ciliate, but the cilia about the mouth | are of a much larger size. a Tie nucleus is a moniliform chain and extends through the central two-thirds of the body. | } The contractile vacuole extends almost through the body like | | a canal. It is much dilated at the posterior end. | | These were observed through the month of February, being | plentiful in one collection and in the aouarium started from it as | | long az it was kept. |] have not found them since. Schewiakoff ('93) i, . : | meenorts them from Asia, Africa, Australia, Oceanica, America,’ || | | and Europe. } + te oe | a? ; fad bas +ahte i 2 a) edt ps a 9q° ua bos | Lg [ dove o¢ eveloun ake Lo abtidi-owt yi clitosti non s pizt dorm et ${ edo Stew ovody , oe ue a! 109 al 79. 65. Bursaria truncatella Muller. PLAXMLF ig. G2. — Se eee ee Se et ment. A e0=)52).5p. S74..P1 KXKXIX, figs 1-2. Butschli ('80-% P69) Pl....LXVie i fi€.. 6. | | ae _ These animals are broadly ovate, very much flattened, and truncate at the anterior end. The mouth cavity is sac-shaped. It has a broad opening in front anda lateral fissure wh¢ch extends from pee left side of it back into the midile of the body. The mouth leads into along, ene. funnel-shaped eames. | 7 The nucleus is band-like and curved, lying in the central part | ‘of the body. There are many small contractile vacuoles scattered through “the body. qi The first that I observed of these animals were very larze- | "so large that they could be seen with the naked eye. Large individ- uals measured ¢.5 mm. in length. The material was from Crystal | Lake, and was very foul, having been collected when it was very “hot and the scum was forming on the water. This was in September, 1899. They occurred azain in February, 1900, in ERoneyard material. ' They are reported by Schewiakoff ('93) from America and Rurope. During February, 1900, another species was found which must have ‘been Bursaria, although it was considerably different from | the above described form. It was ovate, not so flattend as RB. t-runca. tella, and finely and evenly ciliate. The oral aperture was antero- Petminal and the anal aperture was postero-terminal. The body | was so gorged with food that the structure could hardly be made. | out. When some of these food particles were expelled, a contractile | vacuole was seen 4t the vosterior end. The animal moves with a _ Tolling motion. | -& TS2£75 re _— a oe (o7°s ne ye f a =_ 7% : stad ke on ' i all ol he aks oft 80. 66. Stentor polymorohus O. F. Miilller. Pl.ALPig.93, —_—— Se ee ie ee eee ee ee This is a grayish white, trumpet-shaped animal. It is larger Be nie it equals one-‘third the length of the body. It swims free oe attaches itself to bits of algae. The body is highly flexible and contractile, and so varies in shape. . There is a single monaliform nucleus. | : The contractile vacuole is large and situated near the anteri- | or border. Food is swept in by the current of water which the large | adoral cilia keep up about the mouth. | Peo rst found S2 Po lymorphus in July, 1899, in a Boneyard collection made below the heating plant, at which time they were | i ; numerous. I°did not find it again until March 20, 1900. This time | | | i} | | it was ina very stagnant < aquarium started two months previous. | la scum had formed over the top, which was largely composed of l | Stentors,. Schewiakoff ('93) reports it from Australia, Oceanica,’ | } | i | America, and Kurope. eS Se we Sw ew ee I | 67. Stentor roeselli Ehrbe. PIOMLIFic. 74. | Kent ('80-'82) pp. 591-593. Pl. XX¥, figs. 22-23. | Butschli ('80-'89) P1.LXVIIT, fig. 5. | The body is lone. The diameter of the peristome region, | | when fully extended, is equal to about one-fourth of the length | of the body. The surface is finely ciliated throughout, and in | addition to the cilia a few setae are deve loved. The adoral cilia are larger and stronger than the surface cilia. This species dwells \™ a mucilaginous’ tube, and when irritated darts back within the Bi. sould not escape, even by retreating into the tube, and so it left and swam away. The nucleus is moniliform, as is characteristic with this family. reg 2 FMLOM oa ze i The contractile vacuole is large and anterior. | * coeruleus an a stagnant aquariam in March, 1900. It is reported ly | Schewiakoff ('95) from America and Rurope. 68. Stentor coeruleus Ehrbe. Piss Fig. iy Fe ee RS EG OE an) Kent ('80-'82) pp. 593-594. Butschli ('80-'89) Pl. L¥IX, p) fiesed. | | of its bluish green color. It is trumpet-shaped. It attaches itself | Ib to bits of algae or even Saieginacate the surface of the slide | or to the surface film of the water. The width of the pveristome a equal to about one-third of the length of the body. The cilia | are fine and are evenly distributed over the cuticular surface, | but those around the peristome are loncer and stronger. The “fe | is highly metabolic, often extending to its full length, then when | irritated in any way at the anterior extremity, suddenly contracting | into little more than a ball. The nucleus is compound and extends through the body like | a chain of small nuclei. The contractile vacuole is situated in the veristomal The adoral cilia keep uv a constant current of water about i, : ' . seve -otsone BB os agi ie. ; . iY = yn a on B2. e mouth, and sweep everything that comes into the current into dia I have seen it take all kinds of small algae and many 11 Protozoa. Once 1 saw it sweep a small Pleuronema into its zs. One of these animals was one day crushed by the cover glass. Upon watching it a few minutes, it was seen to form three new ee ee ee ee ee oe lot time. S.. coeruleus. was found first in January, 1900, and from that ee ee ee ee on was abundant in several aquaria. They seemed to be most plentiful jin stagnant water. Schewiakoff ('93) reports this species from Africa, Ereanic2. America, and Kurope. 69. Halteria, vrandinella-Muller. P1.X/¢,Fig.73, — ce A ey om Se ee ee ee ee Pent (60-5) 82)0pi, 632. Pl. XXXII, fig se 55-38. Bitschli ('80-'89) Pl. Py. @ a fig. 6. os ee — = —= SSS The body has a truncate oval shape, being cut off at the Janterior end, where there is a wreath of rather large cilia. Long ee tS setae called springing setae are found on the body forming a central lgirdle, by means of which the animal moves so rapidly, darting = and there with a leaping or springing motion, that it is dif- |ficult to make out detail. There is one large spherical contractile vacuole near the |center of the body, and*near to it a spherical nucleus. | This was first found in November, 1899, in an aquarium |Started a year before, which contained, besides Boneyard material, ® some from the Illinois River at Havana. They were very numerous. jit was also found plentifully in the collectionscduring January and i" £ i x] 3 aie : ys iT! es 79 efentiti es a ‘ee | J -t yo tiae hh hppa ei. a] Lb ' Toe pe oo Vm if. . <4 (68°03 nek ; att xTaR ove 98 Vite lun ai nk Yale! yao -Leotage at vhod § 40 gipdlers + atience se aiaks Ll isee es bus bang odd pak: wa iT eiOos Ba if 0d TeBole e_ebnetoe- ane tole tna 7, Bie 2 NG REP vem mat. pees: * bodtetl oes aft hie noon donOd ee é ight limb of the wreath of cilia descends into the vharynx. The nucleus is elongate and band-like. “The contractile vacuole is sincle and spherical and is sit- ted in’ the anterior end. ‘These occurred abundantly at all seasons throughout the year n aguaria and in field collections. It is cosmopolitan. : XL¥Z, 75. ‘Catriche's ttt? vo Vy sur Ehrbe. PLALY Fig.9Z 10e, Kent (' 80." 82) DP. 690- 691. Pl. XXXV, figs. 30-31 & 51; XXXVI,” press 1-8. This somewhat resembles Vorticella’, but is united in social clusters. The bodies are conical, with a dilated peristome. ¥ There is a compound pedicle, consisting of one main stalk which branches freely, and these branches may again divide. A muscular fiber runs throuch the center of this stalk but it is not contin- uous at the places of branching, so the stalk mam contract either all together, or in part. There are large numbers in a colony, sometimes as high as one hundred and fiftm or two hundred. The | cuticular surface of the body is smooth, the cilia being distrib— ute ed as in TOPALOWT Ta’, in a wreath around the peer SS ee i; The nucleus is ribbon-like, long and curved. The contractile vacuole is single and placed near the anterior border. ; | int These were found in quantity in crllections from under the ice i made from the Boneyard in January, 1900, and they occurred again in February. It was reported by ‘Schewiakoff ('93) from Africa, Oceanica, America, and Surope. } 7 16. Ado'thatin' fur seN Ts Claparede & Lachmann. Pl. Fig. ee ee Kent ('80-"82) pp. 698-699. _ The zooids are elongate, being about three times as lone as = —_— a . ~ > a — “al ‘-ored bre, ohm a! Lao’ : - a - - = ae alanis al lawl rans RE. i ne x feats > * ; 'Y 2 Parse ‘ 8 _ one re in * se olfés mah tenddlben »s ese 6 pmwet ean one on? bite (WOT . ry nual, al brayarion ont a4 =) Ligtatee: vd wet tuget: wey, ni a ea04 uP bri sean wid BUD eek OM at os [ en @adis iy and occurring in laree colonies, attached to a dichotomously lk, so that when one part of the colony contracts, it all con- i sts. The cilia are distributed about the veristome, and keen a constant current of water. ‘The nucleus is small and oval, situated near the center of ae eee erectile: vacuole is small and near the anterior margin. bat cus, to which it attaches itself merely for purposes of locomotion. The colontes were found in large numbers. It has been reported only from fBurope. | ¥y 1. Opercularia’ mutatis Bhrbe. Pliapeeeaes De ee ee ee ~ Kent (180-189) pp. 710-711. PL AVI, fies. L2-VS; These occur in large colonies having an exceedingly dichot- omously branched pedicle. The bodies are ovate and are three and a half times as lon. as broad. They taper toward both extremities, but more toward the posterior one. The cilia are arranged in two rows on a ciliary disc which iseattached by one side to the peri- stome margin. A membranous collar can readily be seen protruding Over the edze of the peristome. The zooids may be either erect or drooping, and the pedicle is transversely ee ehiets The nharynx extends to about the center of the body. ; ie noniats is pageivependr curved It is somewhat centrally /placed. | | | The contractile vacuole is single and is located towards the | anterior end. | _ Colonies of these were numerous in collections made in the - - a rie F 4 ¢ _ ; ean eel’ 9 io fe ie. , wt > 4 ie! peluq ines IN ove arise eye. a » a rf i “4 q 5 - is v n . "ojeog\ ait REa¥os J , ide oc Fe egetients " il. > ome eee ate r 7 ht) . EF ‘cired: 642 Ia Ssbe @ tye Saket ey i! ein ites. ef) bra ome Ta4 noo oft tuedh oF me - .3 : : ’ i. “4 woman t st 4 6 oo) feed BD Bleed pun : . > Hi : : 4. fevo! 2! elesic «ft al enon i = me. oy ; . ee |), : é J “lottroi lode @) #iottin oreR eed > . _ For Po Mae Aj aa yay, Uae rae * —— E I : SE etre one last of February, 1900. They were attached to mw ee ee 78. Operolaria stenostoma ‘Stein. Pl. Fig. Peneet Poe 62)" pp. 712-715. Pl. XXXIX, fic. 17. The pody is elongate pear-shaped, with a narrow peristome, The Cilia are arranged on a narrow ciliary disc which is attached by — pné side to the peristome margin, and which fits into the peristome. There is a membranous collar which extends from the peristomal Opening, and which can only be made out with difficulby. The mouth extends down into a oharynx which reaches to about the center of the body. There were but four zooids in the colony eeincee The pedicle was very short and stout, and the zoids seemed to grow richt out from it because the secondary branches were so short. “4 pme nucleus is long and curved lake a horse-shoe. The contractile vacuole is sinele and is placed to one side, near the peristome margin. ] observed colonies of these but eee April, 1899. They each contained four zooids and were attached to the body of ' is reported from America only by ‘Schewiakoff ('93), [though ‘Stein has found it in Europe. | 719.6 Sbhaerobh'rvra pu'si'lle Claparede & Lachmann. PL.XLVUBic. 144 10%. Kent ('80-'82)p. 1 108. Pl. XLVI, fig. 6. Butschli '80-'89) Pie CEERI. fies 10. Blechnanr ay 95) Der 2s This is a very small,colorless spherical animal in the adult stage which is free swimming. The "larvae" are ellipteal. The proto- plasm is ine vacuolate. The adult is provided with numerous slender - - ~< i ee 4 , % 4 & - ne on. a y 7 ray sae “ : : i ee SS eal = p © : ; x eh os ea aie F Jed SPAR Ser ee ra r © wy ot + ‘ = fi mh 7 in, | = a tee Die Dee acl Cg, | oy Slike oe he — . oo As fe 3 o. ; Ses “J : a me : ' re ye ate ah Jct cS ‘q . él 4 % 4 " ’ « P Li ! 7 , La F r AIC Taye ; > iin fit: ‘70 43 hee secon i ( /. fee NE. 2U7Sons | boperked ae BA 4 2e67 aF.3{- . vee aaerc * hwo? ead gileth rir gh: — ey : ’ = : av At 21% \ r) stu Oe Ive fe .8Or +1198" RD ‘grea TRL ate A ae isefe of ast} 7 TARR sit : er : iy fa (eo pteeoe geoitsios, ieee cae rt ‘se oP a 4 mas [esiciile "ential" nor thie beg Odrtw- ererun Orie bebisegeunee ee nitty alfa oe me a - — SS EE 89. bere but Ge free swimming, adults. In March, 1900, Oe ee _— om ct a cc aN a cee lat The rT. The cysts are oueer horned forms with a secmented outer over, and are Stalked and attached by the stalk. A contractile a acuole can be plainly seen about the center of the cyst. al The species occurred in October and November, 1899, and in farch y 1800. co is endoparasitic in THOStyTS, So 1a ee ee ee ee ee ~— = See tor. ‘Schewiakoff ('92) reports it from America and Bloch-’ mann 95) from Europes “a 7 ot /1 erie wiedelat oo a reine Te weet ot suet care en ernie : ¥ y vie @& s Y ae MSCUSSION. The list includes 79 species, ‘17 being Rhizovoda, 5, Helto— } erase iata) | ae Mer las eT sts: and 29, Ciliata: and 1, ae I think the list would be Considerably extended in a “time, and especially in certain groups, since every fresh ‘ium examined and every collection made under different con- itions contained species not before seen. vit has been very evident that climatic conditions, esvecially me re mC err sbt oh the occurrence of many of the las low. This scum also contains PHY in abundance. Then, too, at this time a delicate screen film, known as water bloom, was often found on the surface of Crystal Lake where the water was deeper. This film was caused by the little animals and plants collecting tc the peeane on warm ea and was Teh in Tube e PRESS, — ee me es —- See oliienlentieetenie Gent Say ere on oe ws es es —— ae ee we represented here vather abundantly. |] also founa wareHeetUt and item iF he ta a, Th ney + Peas W ‘ [e@ AD @9£)1%¢ if a a ‘pate ate rey: vitwet. od) 1 164 ma: ud torr ona te wnat t Dae h eT cipLee cect wat mths that ih cr O42 eh GPR tats. oy aed la eos al : Dteiiarel » Oe erie: oo wes aie Teli astnuds 19H9s ewes hey we wees: (5. incke if ‘eT Vee? cal war tH? deg. se he = a ee Se yreres ake “é 4 : pe ; 7 i 7 others persisted throuchout the year. Protozoa in the laboratory aquaria varied also. ‘Those nant aouaria differed Gran those in fresh ones. One snecies ar and be abundantly represented for a time, then it enly disappear, sometimes to return and sometimes not. > one instance, in April, 1899, ‘Ketirfoph'rv's'’ sol' was very nt in a sample from one of the aquaria, and the next day not Ould be found. Whether this was due to the disturbance of the hee 4 taking eae the first day, to variation in ; ch ate or killed the Heemiters, is a ouestion not sn seeel vered. Neither is, it known where they so when a species suddenly @ars from an aquaria in that way. _ Following, are. two tee), one describing the kind of loca~— nin which the svecies occurred; the other, a table representing > seasonal distribution. e y oe oe 7 = i eS 1. So (etna e ; oJ cn & ; vi wee © . bal antes a - : 7 * #1 Oat: Ni Amamcpde ne F ¢ ee ~~ i .. : : A es > 7 y LOVVLIALQYVVVY © g | c : | | + ae AS VAAN Vy lat a aah 4 WWV¥VY aay ¢ ; > | ; ) | } : OY Td UW VE h VOW Vy vroyry = ae | | | < fo os te ae | | 7 WEIL OVAL. (WIV FOI Fl Ie | — —_—— = ¥ y | | — TIE otra. OVO IV IVY | OF a | + = u | aie =e | } | pages ——+ | ae | + | i =| peo 2 a | | = ; PF IIR (a YY yy AAP G | =f | eé IV OVE ry) D VY LY 7G ? . VYyY Fins VL) J { Z, SVIAV TREAT AI LAY eee oS ee ela — meee —! fe ee a ee ese aie al me + a ae eae PS i an eee = india oaks ae hen Sole eae Pere Se are ae ee etek ee | | ALAALAA TA} a 9b. | . Discussion of local Occurrence. The zreatest number of spéciés occurred in field collections. Thalf of thése were common to both field collections.and i. 19 species found were rare, i@c never havin> bean found! foul water. The Rhizopoda occurred about equally in fresh col- sctions and in aouaria and. they were not abundant in foul water. selongéed to this group. Thé Dinoflagellata occurred only in field ections, and one of them has never been reported from America efore. The Ciliata occurred abundantly in field collections and n aquaria, and most of the foul water forms were ciliates. The Rhizopoda and the Ciliata, as sroups, seem to show less reference = ng . % ioe oe ‘ for any particular environment than the others. sy ¢% ~~) ' — us — —— —___. SS Porras! T = ——— pt "7 | U i er Ret 4 i =! | EREERRE TT ERERREED EEREREED cERERAE ‘ a ; ee "| i « SP) ee eS SS oe —_ . ldo, et YOM Ae 7 oT \ a 7 j ————_ = the least, 19. The small number found in any single month I ably due to the accidents and limitations of observation. Small number observed during the summer months is doubtle Jue the same cause. Individual zroups, however, show some indicati: - ~- of a seasonal preference: thus the Rhizopoda 3re most January; the Heliozoa are apparently absent during the warr ew } ct =>] oO — j ) months: the Flaecellata are more abundant c NY) 5 ) ry ° woriisand the Ciliata, during the colder part of the The data at my disposal are insufficient for any extended general- izations on the subject of seasonal distribution. The limits of this paper do not vermit of an extensive con- Parison of the list of species o other parts of the world. | make but a few to illustrate the Similarities and differences of speciés occurine i different le Calitie r rag tw J FO) ig aa ; (oeua TE Bei Ld Dasa oaat B Routers a ee TZ Ss aa __uihtaegellata SS 7 Dis ion of Seasonal Distribut The largest number of species reported in any mont S. Bits (eeefout tote Withewhich My list, is here compared have bear made from field collections, without atténtion having been pv: to aquariam collections. I have found —— | | } | | ~ a, 4 oe wa iw ae Ferro Se ee ——aae ae P 7 a ee: ee. hick paled > pene oer |< is (iw | . < — E A « - _ ‘: 93), who worked in the same stream, reports. This indicates further work here would largely increase the list. Of Hemple's’ S2*were found in the Boneyard; of Kofoid's 25: and of the list of Awerinzeff, only 87. The conclusion is that the Pactozoa are larsely cosmopolitan. irger the lists found, the greater is the similarity in dif-' t localities. The cosmopolitan distribution is due to the ease Be sk: germs are carried in dust by the wind. no ; + Las Ven? Vee (veal 7 7 =? a Pardee a # ,~ S | | \ LITERATURE ‘CITED. 3lochmann, F. 185. Die ‘Mikroskopische Thierwelt des ‘Susswassers. Th. II, peth. 1: Protozoa. pp. [- XV; ]- 134, Pie eV ls utschlis 0. . Pad: "aa, Broun’s Klassen und Ordnungen des Theirreichs Proto-_ 20a. Vols. le dls ana JIT, 2028 pp. Pl. [-LX¥XIX. Bertex, Hed. Do. Note on a-Fresh water species of ceratium from ie Lake of Nynee (Naini) Tal in Kumaon. Atn.Mag. Nat. Hist., Ser. 4, Vol. Vil.» pp. 229, 250. “France, Raoul 7 ) @ "96. Beitrase zur Kentniss der Algengatteing ‘Carteria. Termes— 1 zetrajzi: = aes Min MOlets Pp." JO5-112,.P1. 111, Goroschankin, J. . Bes]. Beitrage zur Keuntniss der Morvholovie und ‘Systematik der chlamydomonaden. I]. Chlamydomonas Reinhardi (Daugeard) ee, < _ une seine Verwandten. Bull. de Moscow, No.1,1891. op. 1-50, P ae peroid, ro "98. Plankton Studies Teta oer Pleordina illinoisensis, a new species from the Plankton of the Illinois River. Bull. Ill. maate lab. Nat. Hist. «Vol. VY. ERs 273-093, Pl. MOON ge KEV “Kofoid, Sh. "99, Plankton. reer II]. On teen eee a new genus of the : family Volvocidae, from the Plankton of the Illinois River. Bull. Mele rstate Lab. Nat. Rist., Vol. Vay op. 419- 440, Pls ell. ee eclioewias ' 4 ‘ ¢ ve Hed aanie eas ant) ae Ope 0(k aoe iV Pia eet at ae as j « weeded aii godt solse Bt —_ Yk oe t vi, ¥ — re | ey otG 7A eae ee WH en tneide no? lanl Vi want + fad et Ae He ots oa Baier Chiciarons of North America, pp. I-XI¥ 1-319, Pl. "99. ‘Sur la croissance suposee de la coauille chez les Theca-' “moebiens. Arch. “Sci. Phys. et Nat., IV. Per., tome VII., 23 pp. haw, WR: oS Pleedorina, a new Genus of the Volvocinae. Bot. Gaz., Wol. x XIX. Pde 279- ORS, Pl. XXVYI ‘Saville-Tent. “780-"82., Manual of the Infusoria. Vols. I, II, III, pp. I-¥; 1-918, Pl. I-L3 London. maudinn, Fritz. 96. Das Tier reich, Heliozoa. 24pp. ‘Schewiakoff, eke Mem. l'Acad. Imp. des ‘Sci. de ‘St. Petersbourg, VII. ‘Ser. T. Miiiiagine. 3. 201pp.,’ Pl. 1-1V. Stokes,Alfred C. “a & Preliminary Contribution toward a History of the Fresh- ater Infusoria of the United. ‘States, Jour. Trenton Nat. Hist. ‘Soc. NOt [5 NO. Ss» pp. 71. 319, Pl. I -YIII. Stein, Pek. 150-178. Der Orcanismus der Infusionsthiere nach Fizgenen Forschung en in ‘Systematischer Reihenfolze Bearbeitet. ABRs | Ls ? ont i -¥; {-206, Taf. 1-¥1Vs 1-VIIT; 1-355,"Taf. 1-¥vI: 1-¥3 By 154, Taf. I-¥XV. LBipzie. Zur astein, Hans. "90, Pur, Morphol: te und Physiolozie der Tubletal orci T's! Klebs. __ '98. Uber die Geographische Verbreitung der ‘Susswasser— Protozoen,) — oma’ { rote, Te le ee we oa AY Bavilh,€ ‘istcn? Cemeiies? a iVety wl el a gel - hey - 2.9 Ay ; ye Pt eats 46> eMaineshael 64 WAP) wheat 9 / yaa Telos? hametey” ay Ler Aa ap Pr er wv x s - - % 7 Gini itt On. at ba 7 ; Sid lac lide pod vy Ay aneey : fi i 4 of) *, op ap eae) n = poll ee oT Sy Oe eee —- -- —- OO — re 105. - Sural-Dissertation, Basel. 1899. 5Opp., Taf. VI. (Jahrb. iss. Bot. Bd. YY¥XIV.).; eff, S. | ir Zenntniss dér Protozogn-fauna in der Umeebune von Ologoje. Travaux de la Soc. imp. des Nat. de St. Petérsboure, rol. XXX. Livr. 1i°pp- 213-251; 262-264. ety Ae : . | A List of the Protozoa and Rotifera found in the Illinois River and adjacent lakes at Havana, Illinois. Bull. Ill. Staté Tab. Nat. Hist., Vol. V., pp. 301-388. ucke, P.M. | | 793. A Contribution to the Knowledee of the Ciliated Infusoria of Champaign County, Illinois. Thesis for B.S.- Degree in Col. of Sci Os of is, 20pp., XX. Pl. Kofoid, C.A. | 194, A Report on the Protozoa Observed on Lake Michigan and the Inland Lakes in the Néishborhood of Charlevoix, during the Summer of 1894. Bull. Mich. Fish Come, No. 6s pp. 16-84, ¥ MCA ERATE: ip Gh aves re’, Aer , A! r, i rs a‘ PAu “hye ee. satea if p-cch NP oe AT ew hewed — e} ' = in 7 “A freee o'o: 9 oft ne ° = iF) e » ° Sele, ae [oe , nly iiaSe ‘ off 7) gttealpeiale " wt ee Ye «re 2S Saar Ye FYPLANATION OF PLATES. Abbreviations. amylaceous body. (Os Ere oral groove. adoral cilia. hats parasites. body. | Poe pigment. cilia. pg-s. pigment spot. ciliary disc. ph. ‘pharynx. cluster. pher. pharyngeal rods. conical projection. | Dere § pseudopdal rays. collar. DS. pseudopodia. caudal spine. p.st.. primary stalk. caudal style. pste. sowie Cowal | contractile thread. tists pseudopodal thread. contractile vacuole. Ds ridge. diatom frustule. Se sarcode. ectosarc. set. setae. entosarc. ; sh. shell. equatorial groove. cnehts sheath. food. SD. “spicules. facet. Spl. spine. flagellum. s.st. secondary stalk. groove. Sis stalk. Worica. Spi. striations. mouth. : “a macronucleus. | ut trichocysts. micronucleus. UeMe undulating membrane. “mucilasinous tube. Ve vacuoles. | erate. Weve water vacuole. neck. Ze “zooid. Yen W ate la blaiei apfa ee. ce: a ease ee etapa - i 107. | Plate I. me ). Amoeba proteus Pig. 2. Amoeba proteus, same individual a few moments later. Plate Il. Pigs. 3-8. Successive changes in form in Amoeba proteus as seen in 10 minutes. . Plate Ill. Fig. 9. Amoeba verrucosa. Z Fic. 10. Same showing pseudopodia. | Plate IV. Fies. 11-7. Successive changes in form in Atioeba’ vérrucosa as seen in 10 minutes. Plate V. Fig. 18. Amoeba villosa. Fig. 19. Amoeba villosa a féw moments later. Plate VI. ‘Figs. 20-26. Successive changes in form of Amoeba villosa as seen in 10 minutes. } Plate VII. Fig. 27. Pelomyxa villosa. Fig. 28. Pelomyxa villosa a few moments later. Plate VIII. Fig. 29. Dinamoeba mirabilis. plate 1X. Figs. Pee yeni vanseheeiranlis,’ssuccéssive stages in the capture of a diatom. Plate X. Figs. ee Geese ee es oaceesive-vhases. | Plate XI. Fig. 36. Arcella vulgaris, latéral view. Pig. 87. Arcella vulgaris, with shell folded. | En : a ; P i Ye ht Ae _ ee = ry a ree A~ a Pee Ph rae . Ab iis ee | oe aie}: "SEE 9 © ql geen ub a aes eres eee * <[peeary iy 70%) Bi ua ree ih by oY oateis \ fon@Eews «tre ory eee SAY een Te Aiy ‘{peetet ontitey ae : rh ims spohiol (\enh, Eee aaitag AY, Meee = « ae a TY Plate XII. Pig. 38. Arcella discoides. 39. Difflucia globulosa. Plate X1ill. 40. Difflugia See rceet latéral view. Plate XIV. fale Difflusia pyriformis, top viéw. , 42. Difflugia priformis, dividing. : . Plate XV. 43. Difflugia. urceolata. 44. Diffluzia corona. Plate XWI. Pig 2456 1Difflosiatconstricta. Pie 216. Centropyxis aculeata. Fig. 47. Centropyxis aculeata var. ecornis. | | Platé XVII. Fig. 48. Campascus cornutus. Fig. 49. Pamphagus mutabilis. Pilate XVIII. Fig. 50. Actinophrys sol. Plate XIX. Fig. 51. Nuclearia polypodia. Figs. 52-55. Mastigamoeba simplex, showing alternation of pseudopodia. | , . Plate XX. Piss 56. Heterophrys myriapoda. Praté XXI. Fig. 57. Acanthocystis turfacea. , | | “Plate XXII. 7 Pig. 58. Cercomonas typica. ares a = ae a ‘a : sth? Arete a, ody ) 25h a? 9 sehen Pe ie ok ese tee Gee ore 7 oe ot aan Fri! | ‘eit pee 1a say a? | eine ate openly t hey wate «alt ks yy ast it) ee = ivan 4 Pe = 7s = . e. 59. Same dividine. Pig. 60. Stylobryon abbotti. -@ Plate XXIII. Be Figs cr. Anthophysa véegétans, branching colony. Fig. 62. Same, detached zooids Plate XXIV. Fig. 63. Anthophysa vegetans, detached cluster. Plate XxV. Figs. 64-66. Euglena viridis, showing metabolic changés. Fig. 67. Buglena spirogyra. Fic. 68. Eusléna oxyuris. 1 Platé XXVI. Fils. Euglena acus. | Plate XXVII. Fig. 70. Trachélomonas hispida. Fig. 71. Phacus triquéeter. Pik Platé XXVIII. Migs 72. Astasia trichophora. Pilate! XXX. ane. 73. Petalomonas medicanel lata. Pie. Ta. Heteronema acuS. Fig. 75. Mallomonas plossilii. Plate XXX. Fig. 76. Anisonema grande. | Platé’ XXXI. Fig. 77.CCartéria mulitifilis. | Fig. 78. Samé, four young individuals in a quadraté plate. Fig. 79. Platydorina caudata. | Ee)" Plate XXXII. Fig. 80. Ceratium kumaonense. we Coa Eee ' «omer rund ‘ ie: ‘AY * . < a — "OOS TARO LAT | OF ‘ mo. Lian e -% “ae pnegy Pero et ag -* erat" eelare aiversiemate oa $ Y , \ es res. ei tet wea a a. -. el 110. , 81. Coleps hirtus. ‘ig. 82. Same, end view. a Plate XXXIII. ‘Pig. 83. Amphiléptus amsér. -/ Plate XXXIV. 7 Fig. 84. Lionotus wrzesniowskii. | Plate XXXV. Pigss5. Nassula rubens. . ws Plate XXXVI. a Fig. 86. Chilodon cucullulus. | Fig. 87. Colpidium colpoda. > Pilate XXWITs Pic. 88. Paramoécium caudatum. Fic. 89. Samé,in conjugation. y Plate XXXVIII. 90. Pleuronéma chrysalis. 91. Cyclidium glaucoma. | Plate X¥XIX. 92. Bursaria truncatélla. | Plate XL. 93. Stentor polymorphus. Plats XLI. 94. Stentor roeselii. | Plate XLII. , 95. Haltéria srandinélia. | Plate XIII. 96. Urostyla srandis. 7 97. Same, parasitized by Spherophrya pusilla. Plate XLIV. 98. Vorticélla nebuliféra. Mise eFsi" Pd re * ; i Leaia a lnel? PO ne oe - ¢ ialG sideqiael abt a A : tne + ec i? : ee eh) Ce erts® etary Orel = 1@ yay! ae Plate XLV. ir. 99. Carchesium polypinum, branchine colony. fy | Plate pa Pie. 100. Carchesium polyvinum, cluster of Zzooids. ; Plate XLVI. Fig. 101. Sphaérophrya pusilla. Pig. 102. Same. encysting. 7. + SOT. Swap ok ss eee ‘ ‘ihe. > Zo. Bl 24¥, 23. 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