MONTANA STATE LIBRARY 3 0864 1001 6071 5 SUPPORT OF AQUATIC LIFE USES IN MCDONALD CREEK AND THE TETON RIVER BASED ON PERIPHYTON COMPOSITION AND COMMUNITY STRUCTURE Prepared for: State of Montana Department of Environmental Quality P.O. Box 2 0 09 01 Helena, Montana 59620-0901 Project Officer: Carol Endicott Prepared by: Loren L. Bahls, Ph.D. Hannaea 103 2 Twelfth Avenue Helena, Montana 59601 February 199 9 STATE DOCUMENTS COLLECTION OCT 1 5 2002 MONTANA STATE LIBRARY 1516 E. 6th AVE. ELENA/MONTANA 59620 ^*> i°0.3Qjta Hannaea 9 1032 Twelfth Avenue • Helena. MT 59601 • (406) 443-2196 e-mail: lbahls@selway.umt.edu February 16, 199 9 Ms. Carol Endicott Monitoring and Data Management Bureau Department of Environmental Quality P.O. Box^ 2^9 0.1 Helena/ fiff§£69ehiQ-0901 Re : McDonaJNjS^reek -and Teton River Periphyton Report Dear Carol,, Enclosed is. a coP!^( W report on periphyton samples collected last summer 'from Mluw^ald Creek and the Teton River. The Teton River at DeriSStjBridge was, the only site that did, not fully support aquatic '^^aV uses based on periphyton composition and community s'tructureSjJ^Ahe " partial support recorded at .'this site was probabl*y due toAasc^ssive segmentation and homogeneity, of algal microhab'sj.tats .- 'T^yWriad of ^bther insults to'the Tetoii' (dewatering, salinity , nutrv^tfe. ..)- did\aot have a huge effect •'.■';■ on periphyton composition anNd^aoVnunity structure . V V \ ' \ ' Bob Bukantis called tN?day to as^ irVI would%ive a seminar, on interpreting periphyton data . Xs^ested -using this report as ■ the basis for discussion, so he ttK^SjjD^ to copy this report for those who will attend the\ seminar 7N7%kV ■ :-'\i ■' .'■-. ..■ ■■:-•-- - % ■■ ' ' Please let me know if you ha^/e any queVtafens ..or. .if you -want the results presented differently^ The fepT^^i's ',.l:iri;';my computer so can easily correct errors or make X Thanks for the work. My invoice is encloseXs&^y ..the way, your agency purchase order lists my telephone numfll&Sas- my federal ID- number. If you need my Social Security Numb er^g^.'.s ^4.7 4 -.4 1 - 94 4 3 . Sincerely, %^ (\jXZ/y\ &zJ)/s Loren L . Bahls , Ph . D . Phycologist Enclosures (Teton River Report and Invoice)' |fe Frustulia bahlaii Bdlund and Brant' SUMMARY Composite periphyton samples were collected at two sites on McDonald Creek and at nine sites on the Teton River in July and August 1998. The samples were analyzed using standard methods for the rapid bioassessment of stream periphyton. McDonald Creek had a very rich algal flora that reflected the lacustrine origin of this stream in the Pine Butte Swamp Preserve. Despite severe dewatering at the lower station on McDonald Creek, water quality and biological integrity remained excellent. Aquatic life uses would be fully supported in McDonald Creek if streamflows are restored. Sites on the upper Teton River (above Highway 221) had good water quality and biological integrity. Severe dewatering at Breen's, eroding streambanks and sediment from Deep Creek, and discharges from the Choteau WWTP and Priest Butte Lakes all had only minor effects on the periphyton community. As with McDonald Creek, restoring streamflows to the upper Teton River during the irrigation season would likely result in the full support of aquatic life uses. The North Fork of the Teton River proved to be a suitable reference stream for periphyton. Floristic richness and biological integrity decreased significantly below Highway 221 in the lower Teton River. Sedimentation and habitat homogeneity resulting from channel instability were likely responsible for the observed decline in periphyton biointegrity in this reach of the river. Periphyton metrics at Dent Bridge indicated moderate impairment with only partial support at this site. Dent Bridge was the only site on either McDonald Creek or the Teton River where periphyton metrics indicated less than full support of aquatic life uses. INTRODUCTION This report evaluates the support of aquatic life uses, and probable causes of impairment to those uses, in the Teton River of northcentral Montana, and in a tributary, McDonald Creek. This evaluation is based on the species composition and community structure of periphyton (benthic algae) communities at two sites on McDonald Creek and nine sites on the Teton River that were sampled in July and August 1998. The periphyton or phytobenthos is a diverse assortment of simple photosynthetic organisms, called algae, that live attached to or in close proximity of the stream bottom. Most algae, such as the diatoms, are microscopic. Although not visible to the naked eye, diatoms often carpet a stream bottom with a slippery brown film. Some algae, such as the filamentous greens, are conspicuous and their luxuriant growth in response to nutrient enrichment may deplete dissolved oxygen, interfere with fish spawing, clog irrigation intakes, and cause other problems. Collectively, the phytobenthos accounts for practically all of the primary production and much of the biological diversity in the mountain streams of Montana (Bahls et al . 1992). Stevenson and Bahls (1999) list several advantages for using periphyton in biological assessments of streams: • Algae are universally present in large numbers in all streams and unimpaired periphyton assemblages typically support a large number (>30) of species; • Algae have rapid reproduction rates and short life cycles, making them useful indicators of short-term impacts,- • As primary producers, algae are most directly affected by physical and chemical factors, such as temperature, nutrients, and toxins; • Sampling is easy and inexpensive, and causes minimal impact to resident biota and their habitat; • Standard methods and criteria exist for evaluating the composition, structure, and biomass of algal associations; and • Excess algae in streams is often perceived as a problem by the public. For several reasons, biological surveys are superior to water quality analyses for determining use support (Plafkin et al . 1989): (1) Biological communities measure our success at protecting the biological integrity of waterbodies, which is a goal of the Clean Water Act; (2) biological communities integrate the effects of different pollutants and provide a holistic measure of their aggregate impact; (3) routine biological monitoring can be relatively inexpensive; (4) the status of biological communities is of direct interest to the public; and (5) biological communities may be the only practical means to evaluate certain types of impacts, such as nutrient enrichment or habitat degradation from non-point sources. Biological integrity is defined as "the ability of an aquatic ecosystem to support and maintain a balanced, integrated, adaptive community of organisms having a species composision, diversity, and functional organization comparable to that of the natural habitats within a region" (Karr and Dudley 1981) . It is an objective of the federal Clean Water Act, and of the state agencies that implement the Act, to "restore and maintain the chemical, physical, and biological integrity of the Nation's waters" (Section 101) . In response to this directive, the state of Montana has developed methods and criteria for evaluating various levels of biological integrity and use impairment in Montana streams (Bahls 1993, Bukantis 1998). The Clean Water Act further directs states to develop pollution control plans (Total Maximum Daily Loads or TMDLs) that set limits on pollution loading to water quality-limited waterbodies . Water quality-limited waters are lakes and stream segments that do not meet state water quality standards, that is, do not fully support their beneficial uses. The Clean Water Act and EPA regulations require each state to (1) identify waters that are water quality-limited, (2) prioritize and target waters for TMDLs, and (3) develop TMDL plans to attain and maintain water quality standards for all water quality-limited waters (MDEQ 1998) . The underlying purpose of this report is to provide information that will help the state determine whether McDonald Creek and the Teton River are water quality-limited and in need of TMDLs . PROJECT AREA AND SAMPLING SITES The project area is in Teton and Chouteau Counties west and east of the town of Choteau in northcentral Montana. McDonald Creek is a tributary of the Teton River and the Teton River is a tributary of the Marias River in the Missouri River drainage. Periphyton samples were collected at two stations on McDonald Creek and nine stations on the Teton River (Table 1) . The North and South Forks of the Teton River head in the Bob Marshall Wilderness Area and converge just east of the Rocky Mountain Front about 2 0 miles west of Choteau, Montana. The source of McDonald Creek is McDonald Swamp, a unit of the Nature Conservancy's Pine Butte Swamp Preserve. Pine Butte Swamp and McDonald Swamp are natural fens that are fed by groundwater flows from the Teton River. McDonald Creek enters the Teton River below the confluence of the Teton's North and South Forks and above the Teton River sampling site at Breen's (Table 1) . McDonald Creek begins and ends in the Montana Valley and Foothill Prairie Ecoregion (Omernik and Gallant 1987) . McDonald Creek is classified B-l in the Montana Surface Water Quality Standards. The most significant source of stress to McDonald Creek is dewatering for irrigation (MDEQ 1998; Carol Endicott, MDEQ, pers . comm. ) . Two sampling sites were located on McDonald Creek, one (upper) just below McDonald Swamp and another (lower) near the mouth of McDonald Creek below the zone of dewatering (Table 1) . The Teton River begins in the Northern Rockies Ecoregion, flows across the Montana Valley and Foothill Prairies Ecoregion, and ends in the Northern Great Plains Ecoregion (Omernik and Gallant 1987) . The Teton River is classified B-l above Deep Creek near Choteau, B-2 between Deep Creek and Interstate 15, and B-3 from 1-15 to the mouth. The Teton River is stressed by dewatering for agricultural irrigation, salinization (mostly discharges from Freezeout Lake and Priest Butte Lakes) , channel instability, habitat alteration, and sedimentation (MDEQ 1998; Carol Endicott, MDEQ, pers. comm.). The nine sites on the Teton River extend from the North Fork just above its confluence with the South Fork, to the mouth of the Teton River near Loma, Montana (Table 1) . The site on the North Fork has excellent stream and riparian conditions and may be considered as a reference (MDEQ field notes) . The site behind Elizabeth Breen's house is just south of Eureka Reservoir and about 4 miles above Highway 89. This site was established mainly to assess the effects of dewatering for irrigation. The site at Highway 89 is below Deep Creek and the Choteau wastewater lagoon and above the discharge from the Priest Butte Lakes. In contrast to the clear, riffle/run reaches upstream, this site shows significant sediment loading (MDEQ field notes) The site at Miller's is below the discharge from Priest Butte Lakes, which is a significant source of salinity. There is lots of bank erosion at this site but it still has relatively good habitat compared to sites farther downstream (MDEQ field notes, - C. Endicott, MDEQ, pers . comm. ) . Sampling sites below Miller's were established to evaluate deteriorating habitat conditions in the lower reaches of the river (Carol Endicott, MDEQ, pers. comm.) . The Highway 221 site had a stream reach assessment score of only 41% of the maximum possible score. The site at Interstate 15, located below the frontage road and rest area, scored 56% of its potential (MDEQ field data) . Significant downcutting has occurred at the Kerr Bridge site, but riparian areas and the channel appear to be recovering; a stream reach assessment at this site scored 68% of the maximum possible points (MDEQ field data) . At Dent Bridge, the stream reach assessment score was only 43% of the maximum possible score, and macroinvertebrate habitat rated only 29% of its potential; extensive channelization and moderately unstable banks were noted here (MDEQ field data) . The habitat assessment score was twice as high at the site near the mouth at Loma, indicating much improved habitat conditions over the Dent site (MDEQ field data) . METHODS Periphyton samples were collected in July and August 1998 using the composite, multi-habitat technique described by Bahls (1993) . All samples were collected by MDEQ personnel as one component of a suite of biological, habitat, and water quality assessments . Samples were examined to estimate the relative abundance and rank by biovolume of diatoms and genera of "soft" (non-diatom) algae according to the method described in Bahls (1993) . After the identification of soft algae, raw periphyton samples were "cleaned" of organic matter and permanent diatom slides were prepared in Hyrax following Standard Methods for the Examination of Water and Wastewater (APHA 1998) . For each slide, 400 diatom cells (800 valves) were counted at random and identified to species using standard taxonomic references. The diatom proportional counts were used to generate an array of diatom association metrics (Table 2) . A metric is a characteristic of the biota that changes in some predictable way with increased human influence (Barbour et al . 1999) . Metric values from study sites are compared to numeric criteria for Montana streams (Tables 3 and 4) . These criteria are based on metric values measured in least- impaired reference streams (Bahls et al . 1992) and on metric values measured in streams exhibiting various levels of use support, which are known to be impaired by various sources and causes of pollution (Bahls 1993) . Because of inherent differences in periphyton composition and community structure between mountain streams and prairie streams, two different sets of criteria are provided (Tables 3 and 4) . For the purpose of periphyton assessment, mountain streams are those located in the Rocky Mountain and Montana Valley and Foothill Prairie Ecoregions (Omernik and Gallant 1987) . These streams are generally classified B-l and B-2 in the Montana Surface Water Quality Standards. McDonald Creek and the five upstream sites on the Teton River may be considered mountain streams for the purpose of periphyton assessment, and metrics from these sites will be compared to criteria in Table 3 . These sites are described in the Montana Surface Water Quality Standards as supporting cold- water or transitional (cool-water) fisheries (Table 1) . The lower four sites on the Teton River may be considered as prairie streams, and metrics from these sites will be compared to criteria in Table 4. These sites are classified as supporting warm-water fish and associated aquatic life (Table 1) . Because of the transitional nature of the Teton River in the vicinity of Highway 221, metrics from this station will be evaluated using criteria for both mountain streams and prairie streams (Tables 3 and 4) . In some cases, natural stressors (e.g., high gradient, low light, cold temperatures, low nutrients) can mimic the effects of Hp man-caused impairment on these metrics. An experienced phycologist with some knowledge of the study stream can usually sort out the natural stressors from the man-made ones . The criteria in Tables 3 and 4 distinguish among four levels of impairment and three levels of aquatic life use support: no impairment or only minor impairment (full support) ; moderate impairment (partial support) ; and severe impairment (nonsupport) . These impairment levels correspond to excellent, good, fair, and poor biological integrity, respectively. Only periphyton samples collected in summer (June 21- September 21) can be compared to reference stream samples because metric values change seasonally and summer is the season in which reference streams were sampled for biocriteria development. RESULTS AND DISCUSSION Results are presented in Tables 5 through 8, located near the end of this report following the Literature Cited section. In each table, stations and their associated data are listed in order from upstream to downstream (left to right) . Streamflow and conductivity in the Teton River (MDEQ field data) are charted in Figures 1 and 2, which follow the tables. Completed diatom proportional count forms are attached as Appendix A. NON- DIATOM (SOFT) ALGAE McDonald Creek McDonald Creek had an exceptionally diverse flora of green algae and cyanobacteria (Table 5) . Some of the taxa recorded for McDonald Creek (e.g., Oocystis, Selenastrum, and Chroococcus) are planktonic and reflect the lacustrine origin of this stream. McDonald Creek had an assortment of green algae, diatoms, and cyanobacteria, which is the typical mix of algae in least- impaired Montana streams (Bahls et al . 1992) . No significant differences in the soft algal flora were evident between the upper and lower sites on McDonald Creek. Worthy of note were BulJbochaete, a pollution- sensitive green alga, and Tolypothrix, a pollution- sensitive cyanobacterium, which were abundant and very common at the upper and lower sites, respectively (Table 5) . Upper Teton River Only diatoms were found in the sample collected from the North Fork of the Teton River (Table 5) . Field personnel were unclear about sampling methodology at this station (C. Endicott, MDEQ, pers . comm.) . Insufficient sampling effort may explain the lack of soft algae and the sparse number of diatoms collected at 10 this site. Sites at Breen's, Highway 89, Miller's, and Highway 221 all supported green algae, diatoms, and cyanobacteria (Table 5) . The number of genera of soft algae increased downstream from 4 at Breen's, to 10 at Highway 89, to 11 at Miller's, to a Teton River high of 13 at Highway 221. The small number of algal genera at Breen's may be due in part to the extreme reduction in streamflow recorded at this site (Figure 1) . The observed downstream increase in floristic richness from Breen's to Highway 221 is probably due to an increase in habitat complexity, coupled with floristic contributions from tributary lakes and streams. Moderate increases in dissolved solids (Figure 2) and nutrients (from the Choteau WWTP and Priest Butte Lakes), a wider range of water temperatures, and an increase in habitat diversity would all increase the number of niches available for algae species. Zygnema, an unbranched filamentous green that prefer warmer and slower moving waters, was the dominant soft alga at Breen's (Table 5) . Zygnema was replaced as the dominant alga downstream (at Highway 89, Miller's, and Highway 221) by Cladophora, another filamentous green, but one that prefers cooler and faster moving waters. All of the Cladophora collected from the upper Teton River was senescent and covered with epiphytes, indicating that the seasonal peak of growth for this alga had passed. Audouinella , a pollution-sensitive red alga, was very common at Highway 89, Millers, and Highway 221. Euglena, an alga that indicates organic loading, was present but rare at Highway 221. Highway 221 was also the only site on the river at which Chara (stonewort) was found. 11 Lower Teton River Genus richness of soft algae declined significantly downstream from Highway 221 (Table 6) . The number of genera declined from a Teton River high of 13 at Highway 221 to only 5 or 6 at Interstate 15, Kerr Bridge, and Dent Bridge. Only one genus of non-diatom algae (Cladophora) was found in the sample collected at the mouth of the river near Loma. Cladophora at the Loma site was in poor condition and covered with an opaque floe. Significantly, Audouinella disappeared downstream from Highway 221, to reappear in reduced numbers only at Dent Bridge. DIATOM ALGAE McDonald Creek Several species of tychoplanktonic diatoms (e.g., Rhopalodia gibba, Mastogloia smithii, Fragilaria construens , and Fragilaria pinnata) confirm the lacustrine origin and slow-moving nature of McDonald Creek (Table 7) . McDonald Creek supported a rich diatom flora commensurate with its exceptionally diverse non-diatom algal flora. The lower site on McDonald Creek had slightly less than half of its diatom flora in common with the upper site (Similarity Index = 46.93%, Table 7), indicating no major floristic changes between the two sites. With two minor exceptions, both related to the stream's origin in McDonald Swamp, diatom metrics indicated excellent water quality in McDonald Creek. The pollution index and the percent dominant species both indicated minor impairment at the upper site. The pollution index was probably depressed because of natural organic loading from McDonald Swamp. The percent dominant species was elevated because of the large number of naturally occurring Mastogloia smithii at the upper site. 12 Despite the serious dewatering that occurs in lower McDonald Creek, water quality and biological integrity- -as indicated by the periphyton community- -were excellent. If flows are restored to lower McDonald Creek during the irrigation season, aquatic life uses would be fully supported. Upper Teton River The attached, sensitive species Achnanthes minutissima ( = Achnanthidium minutissima) dominated the diatom association in the North Fork (Table 7) . Achnanthes minutissima is often the dominant diatom in streams that have cold, fast, and nutrient- poor waters, and sometimes this taxon achieves a relative abundance of 60% or more where these natural stressors are operating (Bahls et al . 1992). Dominance by Achnanthes minutissima resulted in a slightly elevated disturbance index and percent dominant species index in the North Fork, indicating minor impairment by natural stressors (Table 7) . All diatom metrics indicated full support of aquatic life uses in the North Fork and validated this as a suitable reference stream. (The North Fork at the West Fork Guard Station was sampled as a biological reference site by Bahls et al . 1992.) The predominance of tychoplanktonic diatoms (e.g., Synedra ulna and Fragilaria construens) at Breen's may be due to greatly reduced flows (Figure 1) and pooling at this site, or to the floristic influence of McDonald Creek upstream. Two common diatoms at Breen's were Diatoma tenue (7.25%), an unattached species, and Cyclotella distinguenda (7.37%), a planktonic species. Both diatoms prefer waters with elevated dissolved solids but neither diatom was recorded at upstream sites in McDonald Creek or in the North Fork, lending credence to the pooling theory. However, there are other potential upstream sources of these diatoms (e.g., South Fork and Eureka Reservoir). 13 • The site at Breen's had only about one quarter of its diatom flora in common with the North Fork site (Similarity Index = 23.36%, Table 7), indicating a significant change in diatom composition and environmental conditions between these two sites. Diatoms introduced from the South Fork and from McDonald Creek may explain much of this observed change in floristics. Reduced flows and the concentration of nutrients may explain the slightly depressed pollution index at Breen's (Table 7). Otherwise, water quality was excellent at this site and aquatic life uses would be fully supported here if streamflows were restored during the irrigation season. The siltation index (percent abundance of motile diatoms) indicated minor impairment by sediment at Highway 89 (Table 7) . Deep Creek and eroding stream banks upstream are possible sources of sedimentation at this site. The Choteau lagoon apparently did not have a significant effect at this site because the pollution index was still within the "excellent" range and somewhat higher than it was at Breen's. All other periphyton metrics indicated full support of aquatic life uses at Highway 89. Highway 89 had less than a quarter of its diatom flora in common with the next upstream site at Breen's (Similarity Index = 22.46%, Table 7), indicating a significant change in species composition and environmental conditions between these two sites. Deep Creek is a potential source of floristic change between Breen's and Highway 89. The pollution- tolerant diatoms Gomphonema parvulum and Cymbella silesiaca peaked in abundance at Miller's, resulting in a depressed pollution index that indicated minor impairment (Table 7) . The combined discharge from the Priest Butte Lakes and waterfowl-rich Freezeout Lake may be a significant source of organic nutrient loading to the Teton River at Miller's. 14 Although conductivity nearly tripled at Miller's compared to the Highway 89 site upstream (Figure 2), this increase did not trigger a major change in the diatom assemblage (Similarity Index = 51.55, Table 7) . Habitat conditions aside, water quality at Miller's was good and aquatic life uses were fully supported. The brackish water diatom Diatoma tenue reached its maximum abundance (15.12%) at Highway 221 (Table 7). This, together with an abundance of other pollution- tolerant species {Cymbella silesiaca and Fragilaria vaucheriae) , caused the pollution index to remain at a level indicating minor impairment. Overall, the diatom flora at Highway 221 was quite similar to the flora at Miller's upstream (Similarity Index = 51.93%, Table 7). Highway 221 was the only site where teratological diatoms were observed: four crooked valves of Diatoma tenue. Abnormal diatoms are caused by a variety of factors, including heavy metals (McFarland et al . 1997) . In Tenmile Creek near Helena, acid mine drainage has produced abnormalities in up to 20% of the cells at some sites (Bahls 1998) . Elevated salinity (Figure 2) was probably the cause of the abnormal diatom valves observed in the Teton River at Highway 221. However, four deformed valves out of 800 (0.50%) is within an acceptable range for full support of aquatic life uses (Table 3) . The pollution index and percent abnormal cells indicated minor impairment but good water quality and biological integrity at Highway 221. Lower Teton River When compared to criteria for plains streams (Table 8) , diatom diversity at Highway 221 was judged to be somewhat depresssed and indicating minor impairment. All other diatom metrics at Highway 221 were within the fully supporting range for 15 prairie streams. An elevated siltation index indicated minor impairment at Interstate 15 (Table 8) . Species of Navicula and Nitzschia appeared as major species (>10% abundance) for the first time at Interstate 15. Most species of Navicula and Nitzschia are motile and adapted to living on silty, aggrading substrates. The total relative abundance of species in these genera, together with the relative abundance of Surirella species, is used to calculate the siltation index. Other periphyton metrics indicated good water quality and biological integrity at Interstate 15. Specific conductance was considerably lower at 1-15 and at downstream sites than it was at Highway 221 (Figure 2) . Interstate 15 had about 40% of its diatom flora in common with the next upstream site at Highway 221, which is quite a large similarity index considering the distance between these two sites (>20 miles) and the several intervening tributaries, including Muddy Creek. Along with Achnanthes minutissima , the pollution- tolerant species Cymbella microcephala (also a salinity indicator) and Navicula cryptotenella dominated the diatom association at Kerr Bridge (Table 8). The large percentage of A. minutissima (37%) here resulted in borderline values for the diversity index, disturbance index, and percent dominant species, all indicating minor impairment (Table 8) . Chemical, physical, and biological disturbance (e.g., toxins, abrasion, and invertebrate grazing) may all cause an increase in the abundance of Achnanthes minutissima . The specific cause or causes of disturbance at Kerr Bridge are unknown, but channel downcutting and instability may be involved. The Kerr Bridge site had over half of its diatom flora in common with the 1-15 site (Table 8), which is about 10 miles upstream. 16 The diatom flora at Dent Bridge was dominated by Cymbella sinuata (Table 8) . Cymbella sinuata (= Reimeria sinuata) is a free-living and motile species (Round et al . 1990). Although not included in calculating the siltation index, it probably should be included by virtue of its motility and free-living lifestyle. If C. sinuata had been included in calculating the siltation index, it would have resulted in a rating of moderate impairment with partial support at this site. The other major diatom at Dent Bridge was Navicula tripunctata, another motile species. N. tripunctata is more sensitive to organic pollution than Navicula cryptotenella, but is still considered an "eutrophic" species (Lowe 1974) . The very large percent abundance of Cymbella sinuata at Dent Bridge, and the resulting low diversity index, indicated moderate impairment with only partial support at this site (Table 8) . Dent Bridge was the only site on either McDonald Creek or the Teton River where periphyton metrics indicated less than full support of aquatic life uses. The specific cause of the low diversity at this site is unclear, but sedimentation and the homogeneity of microhabitats (lack of habitat diversity) is likely responsible. The similarity index comparing the Dent Bridge diatom flora with the Kerr Bridge diatom flora was only about 10% (Table 8) . Adjacent riffles in the same reach of stream can be expected to have about 60% of their diatom floras in common (Bahls 1993). A great distance between sites (about 20 miles in this case) , intervening tributaries and pollution sources, and large changes in stream habitat will all result in lower similarity values. The site near the mouth of the Teton River near Loma had a much more equitable distribution of diatom species than did the Dent Bridge site (Table 8) . Low diatom diversity, an elevated 17 disturbance index, and an elevated percent dominant species- -due all or in part to the abundance of Achnanthes minutissima-- indicated minor impairment with full support at Loma. In spite of, or perhaps because of the relatively great distance between the two sites (about 40 miles) , the Loma site had less than a quarter of its diatom flora in common with the Dent Bridge site upstream (Table 8) . ACKNOWLEDGEMENTS Carol Endicott of the Montana Department of Environmental Quality, Monitoring and Data Management Bureau, provided the author with copies of field data and with other helpful information about station locations and sources and causes of impairment along McDonald Creek and the Teton River. LITERATURE CITED American Public Health Association. 1998. Standard Methods for the Examination of Water and Wastewater. 20th Edition. A.P.H.A., Washington, D.C. Bahls, L.L. 1998. Biotic Integrity of Upper Tenmile Creek Based on Periphyton Community Structure and Composition. Lewis and Clark County Water Quality Protection District, Helena, Montana . Bahls, L.L. 1993. Periphyton Bioassessment Methods for Montana Streams (Revised) . Montana Department of Health and Environmental Sciences, Helena. Bahls, L.L. 1979. Benthic diatom diversity as a measure of water quality. Proc . Mont. Acad. Sci . 38:1-6. Bahls, L., R. Bukantis, and S. Tralles. 1992. Benchmark Biology of Montana Reference Streams. Montana Department of Health and Environmental Sciences, Helena. Barbour, M.T., J. Gerritsen, B.C. Snyder, and J.B. Stribling. 1999. Revision to Rapid Bioassessment Protocols for Use in Streams and Rivers: Periphyton, Benthic Macroinvertebrates , and Fish. EPA 841-D-97-002 (Revised 1999) . 18 Bukantis, R. 1998. Rapid Bioassessment Macroinvertebrate Protocols: Sampling and Sample Analysis SOPs . Montana Department of Environmental Quality, Helena. Karr, J.R., and D.R. Dudley. 1981. Ecological perspectives on water quality goals. Environmental Management 5:55-69. Lange-Bertalot , H. 1979. Pollution tolerance of diatoms as a criterion for water quality estimation. Nova Hedwigia 64:285-304. Lowe, R.L. 1974. Environmental Requirements and Pollution Tolerance of Freshwater Diatoms. EPA-670/4-74-005 . McFarland, B.H., B.H. Hill, and W.T. Willingham. 1997. Abnormal Fragilaria spp . (Bacillariophyceae) in streams impacted by mine drainage. Jour, of Freshwater Ecology 12 (1) : 141-149 . MDEQ . 1998. Waterbodies In Need of TMDL Development. Montana Department of Environmental Quality, Helena. Omernik, J.M., and A.L. Gallant. 1987. Ecoregions of the west central United States (map). U.S. Environmental Protection Agency, Corvallis, Oregon. Plafkin, J.L., M.T. Barbour, K.D. Porter, S.K. Gross, and R.M. Hughes. 1989. Rapid Bioassessment Protocols for Use in Rivers and Streams: Benthic Macroinvertebrates and Fish. EPA 440-4-89-001. Round, F.E., R.M. Crawford, and D.G. Mann. 1990. The Diatoms: Biology & Morphology of the Genera. Cambridge University Press, Cambridge, U.K. Stevenson, R.J., and L.L. Bahls. 1999. Chapter 6, Periphyton, in Barbour, M.T., J. Gerritsen, B.C. Snyder, and J.B. Stribling, Revision to Rapid Bioassessment Protocols for Use in Streams and Rivers: Periphyton, Macroinvertebrates, and Fish. EPA 841-D-97-002 (Revised 1999) . Whittaker, R.H. 1952. A study of summer foliage insect communities in the Great Smokey Mountains . 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County Te-fp-n 2 ♦ ♦♦if**********************************************************************-*- Cells Counted 4 oa Total Species _ S ' ^ Species Counted SQ? Diversity Index T-Kg*^. Pollution Index Z ,447 . Siltation Index 5~.&>L Similarity Index (compared to site-sample no . I 73" jj - o I ) 4-£> ■ ^3 No Taxon No. C-o11sV<*-[k5> PRA PTC RJ^-o- &^| ru_<-- -£- lH><,-i-P-g Lq-u»— 5-rv^wTJU>C. £4Cs> 3o,75" ^4 ■CWi |\ Za^ tjTjgg | Un-<_^_- bjTfAj l S \rXJ^c\-a^ z& 3.ZS ■=5 ^l 5.75- r r^f Lv-i jua^s: P m k-l^.7) k7 8.57 f~. (C-c-vuS 'K^-g-^-5 ^' u-e-x/QK^i &>£> 7. So 6pALflJ^2 S-g^k^Li3^ Cr^eS 1 fU^n-cJ -T^--f"^^— ^ Ull O.So Aci.ki^x>^s ■^S'^A. 45 6,00 QaeAfcaJgLgilo, iH±£^a cg_^> kgtjg^ .75" 0- -SiU-c,ua^A^- 0.5"0 2. H^ax-ff^,. lsUw«-Jll^V Z3 Z-67 /Wv^k^ 0.2s- z iryki (L^a ULUlAg fltl 1-37 p>k*Oft m/iAi;^M5 i 0.4.1. /^^Lo|)lxu^,fl_. ^fJ[M_Cx^ck-U^ 1 X 1.12. (STy-yvi^lMrvveiVl^* j >/V^fTAjC-<«JK<>vv. 0.Z5" Avwf? WtrtA, (U.<&c^liUl_ O.G.Z. 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County -f-, «-1-o->n ***************** Cells Counted ******* ************************************************ Total Species Pollution Index Diversity Index Similarity Index (compared to site- sample no Species Counted, Siltation Index No. Taxon No. eeaajwkg PRA PTC 1_ 2_ 3_ 4_ 5_ 6_ 7 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 % Ull c<_ (^M-^-^-^t o . iz. §^j l^l^A-*^- ^-a^U^-Sl I I C_£^ _ O.Z6" -D j «oi-p-»v\-n. , "IC/tiaa.? _ 0.2S" Z- l^> i j- 3 :> cXul*-^ \ta1' 3 Avv^p k^LA, &D - C!> ■ A ■ \J g-VJL-T" O.Z.S O.IZ. A?-v^ U^yiA. ^LLgaJ3a.l sc 4 7Z. Kan.naea/09-98/250 SITE SAMP i' AX A CHEM DIATOM PROPORTIONAL COUNT // Page No Site-Sample No. \ 75 4 Ol Notebook No. Water and Location M g. D-d-h^U o O^ejoC , icuj-c^. (au,c^_ /■vt-^-^-H-t. J Sample Date 01 I "Z-Z- 1^8 Community 3 . Substrate KJ Collector/Aaency C • gVidtcp tt" / 'Mfrfcff. Project TWbl_ HUC ioo'b O'LOS Reach No. . County ^7 I «.f a-*n •*************+**++*****+++*•********+**********•******++++*************»** Cells Counted 4 CO Total Species. (eZ Species Counted 6o Diversity Index A ■ S7 -^ ■ Pollution Index Z ■ 8o7 . Siltation Index 4> -CrO Similarity Index (compared to site- sample no. I 7 5" 3 c./ A^.^3 No 1 Taxon No. -Cellc^cJois PRA PTC W^lfeiU^ b4hr^Ax\ Q^. 22 Z.lS 2_ 3_ 4_ S_ 6_ 7 A-*"v-l l/un<3-. XJ> JLlp^JU-l^^ WMMMM 3.37 4. LOvi^ c^— £>, IZ A -^ cf . A . tM-tuJ-g- o,So a* (-A-J^r\j-LAJUI 3<\ 4.87 ^3 ra HHMM 3. so •Fy^^i Lt/ui^ l?v^(Sn<-^jhv_, 7- 75- (9) T^ pi^^ua>^-- Zo )S~,ao h (SfrvuS'l^^-e^LS /3Z EHMj Ik.ifo 10 F7 lL^>j-o-^>'+T^A^-^r-r\ 3.\z li i^d^A.. 4jbj; 'd>— 4^ s>z. 2. 12 A= A I^CiL- t: w 1,87 13 "^ <-Vl(»-Ufc-C cr-LfM-ld I ffl ,7S 14 ft. k^st,J>J MJi >r n i. z,£,z 15 16 %*..*!&> A ^ U-J H *v_ ■^o^n Lgk t^U^HVI^ Hi I. So Z,37 17 18 19 20 21 22 23 24 25 26 C^jdotUi^ dl ^XCsAJfr Ufl^Xfj^ o.$o A^-L?a^o SXVl^- C^LA-S<.(P c^nr_J7T^-HT>_ ^.3? A ■ T£iAUAsL^t^ o.zs A . ts La^JL-c£>>~. . o.31 ktbnA*M*s nu^Ar '^'W^ £(* A-^o (<^-yv^t^^rrvP^*^^rv_. W)'KU', I <3.G>Z- C^n^Ul^L-- dM-4_c-^->k^-Lx H .zS '^Wt^j-is-g-Us-l^-^ 9yy-AJrU^^ 10 Z5" C-A^r^AM^ O-JLfu^cfns I 0,37 C^kJ»^.cia1 /*- YK cwc s cl^ I. 37 28 K) . by la ppImJ /■ 0-S7 29 >0. p v^x^^-c ^c^cn rcM. ^vox_^ 30 K) H2- f. jjOgJ n. 1 rfiY- I^Lt- <9. 25" Z. 31 N ■ c **ff tLtUL a j 25" 32 M. -k" p-^o^r € 0.75 33 M I qn*> O. (Z £ 34 AO . m.£/V>-KL CsL^JuA^L j o.zs z. 35 N ■ UU4A^t4^vvv(gO' £.25" 36 M o.z^r z. 37 Mir^s^-A-o^ -go O.TS Z. 38 Coi /vA-4M,.ll/ >OM-OCO ' at 25" Z. 39 s>afg=*=&£Ss cleXic^J-i'ss O.IT. 40 (^Vrv^lvrrU PvUL- IJ3- Y-a^-(S^v_- M > j- g s cJa^la^ ^^idJrgjh^-- o.iz. 41 -£- 42 M ■ ULCl^v^ d>. IZ. 43 4<^^uvrfa,s cie^x^^ s,37 44 /4ot^?U<^(-€UI/U»1^' -p ■g-iU^-OC-tf^w^ 0,25" 45 £ -f k^fr>vl tedB — 4-w^L*-!.* I^UMAJ'/n^ 1 5 O.IZ. 46 £ 0.37 47 A C Jfl. '/^aS^Ji S jgfgjg uJf \&rU^ o.So 48 /4r/i tt^asJpjm La^ct&Uj MM 1.25" Z. 49 £l ^ jl-tr-~v», tJ^, 1 o.lZ 50 CUjrwA^if a, iMiaO^A^ O. II 51 aU to Uv-Kg_ c 0,7.5" z. 52 r^[uyvKjd*^>*i^ki s (-^.crr ioq.5 M 0.07 ii_ C^uaaf=yLSa ^ i ifi^s t<<-cA^- £>.£7 Notes/Sketches/Additional Taxa : S-L_^U- (^dL--i-e_JL^ &Z./tz.lqq y<. ^. B« tfa/inaea/09- 98/250 SITE SAMP TAXA CKEM DIATOM PROPORTIONAL COUNT ISA Site-Sample No . _ Water and Location Mc fc> g-n^l d Ofejj\c, [ Notebook No . II Page No . 27 ( *-€.rt^i y^&^—kW ) Community. Sample Date £> ? / zz / 48 Col lee tor /Agency C ■ E*\ d i c!o ft" / M 2^> fc"(? . Project TrnbL- Hue f O 03 QZ-oS' Substrate K Reach No. County, k I <2- rcr>-\ *********+******+********•+**++***+*+*+********+*********+*******+*♦*****♦♦ Cells Counted Total Species Pollution Index Diversity Index Similarity Index (compared to site-sample no. Species Counted, Siltation Index No 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 Taxon No. eet-JrS.Vgdi'g.i PRA PTC ^-e_- . Z3 o.+37 c.o 6.6.- >C - 373 Hannaea/09- 98/250 SITE SAMP ;axa CHEM DIATOM PROPORTIONAL COUNT 747 Notebook No. If Page No. -2-Q Site-Sample No._ Water and Location Mq-t-Ha fi^iic-TeJ-v^ R.n>e-v~ v\Y\ <^-o-^M^ ( /\ ^bo if 's ) Sample Date ^7 / 2.Z. / ^8 , Community & . Substrate bL Collector/Agency C. fcCwvcUccft' / MPtS g . Project TMbt- Hue |QQ 3 c?2.g ^~ . Reach No. . County Te.4-trv-i *************************************************************************** Cells Counted A OCD Total Species _A 1 _. Species Counted ^G Diversity Index 3.574- . Pollution Index Z-.Q^O . Siltation Index & . U7- Similarity Index (compared to site-sample no. ' "7 4 3 - ° I ) Z3 ■ 3u> No Taxon No. Colls-V^J^es PRA PTC AclU^u* 4W-5 kyu^x^4<-£Siw->^ ^44- ,5b, .So 2_ 3_ 4 Gl 7 ^ . biU-ff imA-g— 44 5,5o Soi^-X^^a Vu.W^U'Ai £>. 5- uj ^-^. <0.5"O (S-grA^-^'lMTH-Ci^w?^- ■p'-<-yv\ 1 LdUo S4- /o.5o v-A t\ Le^ a± 1.75- £ O-M-Ci u^S )— e^^v-oc^^V JHTMff .37 2. U-&{-^_Wy ( - <£ . l"\.A-l-(-t^eot-uA.Uo ) 75" 337 @ 6. M 0.75" G« c-C-zrw-e.l.S P LgLX-g-M-^-v-^La- 5 o, 12. 11 12 b-Ol-kr-^JflL- "feu^d^ 0.25^ C^w^jy^dla, qjPAt/K^. 1-25" 13 14 15 16 17 18 19 20 21 22 23 24 25_ 26 d- •$\*JU<-&Jtc^-' 34- /j,z£- 6. cUilc*jLi. £- A^p^^t^ CrO-otA^ ^Ift-U^ y^M-A-<3 aLgrv^ M I.OO 4 aj^v^uux^ oa^c^.^ & uri{ o.S7 D~C-K H-t-QcA Le>— sSLaja «^s -£- tLsSsi \^\Xxi-c^o CXeUt,^, t£l fzofc ■L 0. 6>Z 4 a '25 Q^jSJ^gJl^M i^-q^vo ci^Pk^-la^ ltt£ • 2S" 2. 4-U-U^y5, g^LA, Ltd I C<*_ ^,£7 Qnr^-V) UervU n^»— Pol^iiac-con El 0,75" Site-Sample No 174 Page 2 of 2 No. Taxon No rollO'VoJues PRA PTC 27 N a^JA£j^Le^y . ZS 44 ficX^aaa^kg^ -^p. # z c£ Usui's, &U aj-tnuj^ Ia^^p^^lX,^ a 2^' 45 O.IZ 46 4c£ ^WHaT>^-5 $p- #" ! C-f- (^wvX^^u-^ ii_ 0.25 47 CUi^i^-e-ii HUAUv J=_ asrfl 48 ($-p-»^f3 U&nt. HA^- ^7 <5, E^r 49 Lm^wIa- gl£> -tA_jL*_^ 0,37 50 rVgt-gi i Lft^vc^x-v p ' y\ K-gQ-g'— o.zs 51 Col p^jla^s £ P ■ C^fcLU. ) o.zS 52 A^L^c ill fl.sO 5i ^M^baJCA- ^p : Lfi£ p^-g^^^-iT'^-'s j O.50 Notes/Sketches/Additional Taxa: S>w=U_- ^n.^L^^^L^ ^/o?/^ ^u j_ . /2>.a-Us kcTvuj. HZ. 3fi /5" 1^2.2. e-P. A.. ^v-o^vlJox^-cs^. S^vvfLe iku^m^CuUI*-, c^IwjU— £j <"**< Pollution Index: PRA PTC No. 1 O .LrZ. x 1 = PRA PTC No. 2 4.74 X 2 = PRA PTC No. 3 ^H.lf- x 3 = = 2.^4.02. Divided By 100 = ©.^2- ^•48 2-63.9Z ZA4o Siltation Index: PRA Navicula sp . PRA Nitzschia sp . PRA Surirella sp . o.c^ + * + o.o c> . 6. SC : ^-ikS Hannaea/09- 98/250 Site-Sample No.. SITE SAM? TAXA CKEM DIATOM PROPORTIONAL COUNT 748 o I Notebook No. Water and Location ' -v-jg_a_^ ' s QaJgp^-e. . Page No. -2<^' Sample Date Q7 / Z3> / °\Q , Community & . Substrate. Collector/Agency C- ■ g^diooff' / At £*= e? . Project Trvi p i_ Hue ' °° 3 O 2-c5~ Reach No. . County T^fo — > M * + ************************•********** + ******* + ***** + * + + ** + + ********•*•**•»•*»** Cells Counted 4oo Total Species _ (pi Species Counted 6>Z- Diversity Index A • 4^3 . Pollution Index 2.. 4 2-2- . Siltation Index 2. .73 Similarity Index (compared to site-sample no. 1 "7 4 "7 - o I ) Z 3 .3L, No Taxon No . Ge-HrC Valves PRA PTC CD- QcJl i*-tft^w.-|-Ufl-& 3 t s S i w\u^-~ ^4- H.75" 2 A. b lO-^^O kiL CJ_M_^-^- UflU I.OO uJ H-0k_ 15*4- HI 13.25" S. ^^y^C\AJ^CJ_C\^UK\i \.Sc C~ir* bjdVc $t* y*J—Jb<—^- TzMvuA i C£tiU-~ex\t A-o S. OQ WMM LIS AcJ^hjwsJtUjl.<> L&wj-jP^> IaJh^- 5- 4^L*-e-o 'lt-^1 0.2S 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 /4- is \-4^^Jb~c^*-' Mm (•75 /4-wv-f fujCcA^cJL^ 0.37 C^joL ■UjU, g^$ h^uix^-*. j53 7.3 7 £a CX<»v-g^U ft L*^cc^j/-^-^L^ — o,^o hmi rr^vi.g. t e*-^~f s& 7.z$ rv~MU. LusT*iasY\s\ O.^C 29 0. 12. 30 (5. 50 31 0,37 32 £> I p LcryilxUL £g (•^jr^JLcgj cj-Cj [ C; o.z> 33 fV5 ^ gtxlX^»^ c-g^jrc S £ <^t->wg^ M 0,87 34 n^^Lf.Kfl sp- cP. Web- £.E5 35 O.Zb 36 M^vi d/t^v, CJE£&±igjsLg O.Z5* 37 Q^clold,^- fclj-yvi^ 0. 12. r I n KA>,L£t-LX_^_^ H^U-OLgrSJ-gt^x-^O-y-) (7.1 Z- 39 GrTrr^-^V^r^JLV** £t/H-ly<^> rP^r^Ov^ o.zS 40 Kjjj^^OM^a: P^omxvlxaJ o.25 41 Mlf^^^^^-^— V^-C^l [[ LLt^~\ -£- 42 K^)- cltsSi f" Ct.'ES 43 N "pgOu^g^— ffl o.S7 44 M . C-AvvX-io \-M^L- O.ZS 47 (2 , g^w^ UajcjljP k-gq O.Z6 48 4-laLax-o .-tz H^-ci^l . nil l.^>0 49 £=>--cry>A_l U^-yutHA-O— p i>n^JUxJLc^vt^_ Ml I. 2b 50 ($-p->~i-j0l fi\-*-yyiA_-L<^t "^ urnr .25 51 d^k-clL^- pj sj-^ .Li „ -£- 52 *TVK^^£i^=. )^-^>->^vr?t>t^\_ o. So -5_3_ A I >t<2xuX-fOS i S Notes/Sketches/Additional Taxa : Sl^SU. c^uxL^-tjt-J2__£>2-/ce>/qc\ \pu. k "S>c«JaIs Pollution Index: PRA PTC No. 1 X 1 = PRA PTC No. 2 X 2 = PRA PTC No. 3 X 3 = Divided B y 100 = Siltation Index: PRA Navicula sp . PRA Nitzschia sp . PRA Surirella sp . o. WL- Z + •'1 o + .o Z ,'/ jpo-cj^ 3 of tfinnaea/09-98/250 SITE SAMP TAXA CHEM DIATOM PROPORTIONAL COUNT Site-Sample No . j 7 4-6 Water and Location I ■e-T'o-v-i. Rj Sample Date 07 / z3 / ^8 . Community Collector/Agency £ ■ £ -^ drc-off"/ M D t=6? . Project "Trno /I pl Notebook No . _ / Tg Page No. ^ ° Substrate M HUC_ \OC3c 2-oS Reach No. County Tg-Tcrv-N ♦ IT************************************************************************* Cells Counted Total Species ._ Pollution Index Diversity Index Similarity Index (compared to site-sample no.. Species Counted. Siltation Index No 1 2 3 4 5 6 7 Taxon No . ■ Colic- vkjues PRA ?t:~ Co. <^T7-rKjgU^rvU>tA^- •Sp- C^LWjriC^gCi,,?^ ^jl/dU.) 0.2S a 37 Kj ) ■)- -^ ^ g-^-g^— ^W^Pu^fvu O.ElT Z- 4^-Uu^o ^-g^-^3^_ sp ■ cJr. cj-isn^^-c, £>.!£ M ,a^M-CA^.lA . T~t*~./Z ^4-o*-Lg«-X-o~S Xx^La . cr^si 0.37 rm^i LoA^-gk^- c^lo 4-^~>^e-v<-s ( s 0.2.S" CU-UrrTXUU k/M^j l(uU~-\ O.Zlf z. 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 2G »M CUMf IU 11 a.Z5" 0.Z5- O.IZ O.ZS Z^ Site-Sample No.. 74 4 4- Page X of X No. ,__ Taxon No. Ge-Hrs V«.li/*s PRA PTC 2^ Lf 26 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 # 45 46 47 48 49 50 51 52 53 i Notes /Sketches/Additional Taxa : s(l j,S8 X 2 = PRA PTC No. 3 ^4.3>Q x 3 = = 2.4. z., I & Divided By 100 = 2.IZ- + lo7. It, I3 2.*q0 2/|ZZ Siltation Index.- PRA Navicula sp . PRA Nitzschia sp . PRA Surirella sp . o . CfZ. o- o 2 .7 SC A-qc Hannaea/09-98/250 SITE SAMP ;axa CHEM DIATOM PROPORTIONAL COUNT 7 4*\ - <=? I . Notebook No. 1 Page No 3 Site-Sample No. Water and Location 1 ^-tc^ Ri ^-e^_ Cg^ 4iC|U^>^^i g^ (2- /4/] c fc_gL^f Sample Date £> 5 / °7 / ^9 . Community S . Substrate. Collector/Agency C ■ t£* d< g-otf"/ Al D£c7j . Project T7ni)i_- HUC ) C 0 3 O Za 5" Reach No. . County Te-4-rrv-i J2./ /^ ****** + ***»********** + ******* + ******** + *** + ************** + *■»•******»»******* Cells Counted 4v0 Total Species _ ^^ Species Counted -5"3 Diversity Index 4- 6>og . Pollution Index 2-^7^7 Siltation Index So. 81 . Similarity Index (compared to site-sample no. 1 ~7 4 6 - oj ) z.^. - ^U . No 1 2 3 4 5 6 7 8 a. Taxon No Q^^G^'cdu^s. PRA PTC £ occotvUX ,u cAjO' 44 5^c (2- p-g-^.c^^LM-^t^ as 4^1 f-ytu.1 l^UiU VtU>.CUU^»-t lUI =rt i«««i)(f« -5.00 ■F? ULjO \-os4^^Q^e-r[ O.^O t~- C, I 2- K^-P-lCCS p tlXvU^L^ jL. 55 [0.37 10 Bf t 4ke^vu-^- ^ir .y 3E A. DO £>-g-»>v»~jdU^r>*-<.; **-&— oA- 13.00 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 ^ 7^ 24 3. do <=> • oU^rztx^. U->n ■£- C^^fcxK ^fkWuTS^ JW LIZ. £ C «-v^-e^crj> j?U**J ^ WMM \.1£ 2 'Sxx^mi' Uft^. L -P- i"^ i-^ -^ hot Z.kZ ^'■nk-ULa Sf»vtA^4^ - Si. a£ C*aiak&iifl **-&- ■^rVim^ mi (S-crv^ — -p LtrA4.t ■£- Jfri .2^ 2. Sice-Sample No 17-s c?[ 4- Page 2 of / No. Taxon No. eoi^aVW^^s. PRA 27 ^ gu_>-A Lu_ '^-d u£U- mtufhiw ■IWJHUP m 3.67 29 R> ** u ^gL^l<-^_ jL J£i A- A.zs z_ 30 Ki . <^d\a^iJtUi '-a.*c< Ml 'A \.S0 31 N . v-lvuS- WJfA. o.Sp 32 M hA^>u,S ccuxj^L. -&■ 33 M.t o. 5o 34 AJ IHOMOKM 3.37 35 M. v/iyi oU^-Lev. V- Vc^s-i-eJ '(gv-f-ea- O.I2 z 36 KJ. cU-e^g'3 /[ (5.25" 37 N). c^ SI 0-5Q 38 M . /-vi^ma^s cxa,((^_^ O.Z^" 39 M /-Vl in lrw£0 lgi— Mil l.OD 43 K) IVLCgVLSP' P m t l.fcZ. _£_ 44 M £ -L£-x_ C 0. uz. 45 /0 pgl-(£^_ tJBtJBg z.uz 46 ^ ■ uv.u.lq^ 11 £>,37 47 M. i£Ov-v pbuJi- O.So z. 48 w. ? g.Y' >v\ i iax^O i O. fi 49 asLedftaik^s lavJULZ) LcJr**— 0.37 Z. 50 (-r&^JD \*~Q~r*UM^-i*- V1 g.H_f* ''^S' o.zg 51 p I YbL~y*J-+~ &-ck-\A-*y\p^urui. i n.cmc£^\,s i s Hi £.57 £3_ ^ «-( it-UJ^ I?' '*q^o iL<_^ *■ Notes/Sketches/Additional Taxa : ^>l > eU_ r\ l^i l>*a_ (g>, Hc^^-^^i g^ Cg Mgk^^js Page No. ^ Sample Date Q& / &7 / ^P, . Community E Collector/Agency C ■ fc=Vidi co tf~ / Al£>&<3 . Project HUC lOo 30 Z&5~ Reach No. . County Substrate N r^t>i Ifejn g-v"l *********+*******+****•*****+******+**************•**********************»» Cells Counted Total Species. Pollution Index Diversity Index Similarity Index (compared to site-sample no.. Species Counted. Siltation Index No. Taxon No. Qellg^^es PRA PTC 1 MlT^Scivc<^ T^vrtc* ( o~— 11 0. Z5 2 N • Oja-c^ u£ |-F(jU^La_- 0.12 2. 3 HI 0.50 4 M ^UMi^wi*- lfc*1 z Jul f4ixAfrd-f I 3>^ C^VL^cl^A^— 0.1$ 2- 7 I " J / fc o.iz Z_ 3 1 1 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 Sit =»- Sample No. 1 74 - O 1 4- -f- Page /of/ No. Taxon No. eeHrS-vUlc/as PRA PTC 2 7 i f 28 2 9 30 31 12 33 34 35 3 6 37 38 39 40 41 42 43 44 45 "• 46 ' 47 48 49 50 51 52 53 i Notes/Sketches/Additional Taxa : Pollution Index: PRA PTC No. 1 (*.U2- X 1 47 1)2. HI ss PRA PTC No. 2 2g, 8! X 2 = 57-CeZ- + PRA PTC No. 3 M- 57 X 3 = <^3. 7J _ 2-57.9^ Divided By 100 = 2.^7^ Siltation Index: PRA Wavicula sp . PRA Nitzschia sp . PRA Surirella sp . is.q7 l4-.+84- + — P So. £ = i zoo tfajxnaea/09- 98/2 5 0 SITE SAMP i-AXA CHEM DIATOM PROPORTIONAL COUNT Site-Sample No . ° & ^5 OZ. Notebook No. L Page No. Water and Location lej-p^ |2^t>-e^CiP M^fev's b-gi^w Pyu2.sl-BLA.ffe, L«-kg_ Sample Date 0& I os I °>& Community ^ . Substrate. M Collector/Agency C-- fc-^di c-eff / /V1 ft fe-Q . Project T^ b L- HUC 10 0 3c 2-Q^~ Reach No. . County T&fan **** + + ******** + **********■ + + ******■*** + ***** + ********** + ***••**************** Cells Counted 4^0 Total Species. £> I Q zL ■ Pollution Index 2- ■ z^ 2- . Siltation Index_ Similarity Index (compared to site-sample no. 1 ~7 4 3 - O1 1 ) 31 ■ 55" -EJ No Taxon No. CGllaV«=J^es PRA PTC f\ dw^^-J^JC^ J^^iMAyd-lSSfW ■JH^_ (p3 2^,37 2. 3_ 4_ 5. 6. 7 (Cv-i ^ylvcl^A. i^^^i^<-(U_Jk^S *■ Ooi WA.lt> aJfcJL >^mxi-c\ H 0.75" Z_ C- aj 0.75" G>-VW~JP Wo~Y^L^^\ ^VU^L>H W^a^ J^fL 8.t* T~V~g*-^ i Lu-y^g^. V ^C^-^i«-€^ ^£_ 4.37 2. b^^jkw^. iLLeuAL JHt 1.00 Rj^tXX^CTS KJk^vU^flt^ C^u^M>c»ch? 1.07 9J 10 11 12 13 14 15 16 17 18 19 20. ? 22 23 24 25 26 £yt>w^ U^GvUj l*-\J&~- 13 [AAA & ■ C)[^ jJ-tX-UUA^y^ £. J^-U_ 2^ 3.12 Mvy^U^-T^i, Z> £ J/ A c**i j , ^ ^> a 0.75 UAjZAAJ^cSt S O. ZiT Cc|KnL<- &_ Y*A-C\r,w^OVCL^4 I JJ^A— (1 0.25" (^rv^^^t^vL^u^tc ^^jcx,c-£^>^~Ky^\ -R- r/"a^. I ( QLy^L-q^ CtM.S f-lH>CjZ-|A_^ ^ A ^P^uJ>Llua-A_^ ? ^Ucc^-cLo^ .D tftiTrwicc \/i>ilc|a^i_ il o.87 o.i 2. z_ Site-Sample No ce>. vM_X-, K' £>.&■£ 36 k-l pUrnxx^L- p-^-t-JLQ— ©.37 Z_ 37 £ Uo-a aJLa-Jx^ gx^fe I WO— -£- 38 A-<^i-^X^? S-lA-Vtt— j £>L«^-c(u_C a. rz. 39 J-lX-Kj/- 1 C-C^Jxv^ d-vyc, £.5o 40 fi *"t l^T^t^ gw Le^4trST Sf'^Xt^-yi £>.|Z 41 ^ir?<,cXu_^ ois_gj p<^-j-gw E o.75 42 43 kL M. -t* L ■c^- \JUK JW 1.00 fl.gp z- 44 w HO 1 .5o 45 ^- Ctn^buJh*- (~ >Q ■ cs^l -P- 46 )0 . tvjU^l^Lg^X(-^^ -£- 47 M ■ LgC-C^LA_M_>v^ -£- 48 Kl. sp. cf. g-tA-ggv^J uu o.z-Z z. 49 w. g^v^g^-S y -^ 50 M. S I. cf HMM-u»C^ ^-£-£- ^'z-/^>^[e\ei bt^kls Pollution Index: PRA PTC No. 1 By X 1 = X 2 = X 3 = 100 = PRA PTC No. 2 + PRA PTC No. 3 + Divided Siltation Index: PRA Navicula sp . PRA Nitzschia sp . PRA Surirella sp . rkc.e 3 cf <-! HcLonaea/09- 98/250 SITE SAMP TAXA CHEM DIATOM PROPORTIONAL COUNT g Z- . Notebook No. \ Page No Site-Sample No. O 8 ZS" Water and Location TnJ{g LcJd^_ Sample Date 08 / 0-^ / ^. Project T>nh(- HUC \OC3o 2-C 5" Reach No. . County Tgl.\-q-y\ 2-Z. ********************+********+*•** **+**+****••*****+****■ •*********+*** Cells Counted Total Species. Pollution Index Diversity Index Similarity Index (compared to site-sample no.. Species Counted, Siltation Index No. Taxon No. Cells Values PRA PTC 1 M &-+JA. LajSj^s ^i^AJLXT^-- wr I I. DO 1 2 S^^\JuJUu^_ (J_gJj~C-CkJi. & f 1 <~-e^ O.lZ- 3 HlT^Ou^i/ {-Ota."-LC^>(c^ i OAZ- 4 C-j--|haJkJUx. CLS «J'm^ i o.z$ 5 / . 1 C1. . c^^& pi It^^— OAZ. z. 6 N (umulU/ f" c k G-r»— - l( o.So z. 7 Kl\t-?5cU*^^ ^p • II o ,zS~ z. 8 Prn^fL*i£<_ UJ?M co. - OAZ 9 N i r^? s clvcc^ i?£^ !oaL. OA2- 1 10 f r t II o.i& z. 11 Gryrvv^\/^OVJLiM-A~ "UlU£A;ii*M- I 0, \Z 12 Co|^^(L^ -,p- I 0.\Z- 13 14 15 16 17 18 19 20 21 22 23 24 25 26 Site-Sample No D£ 0 2. 4 *r Page / of / No. Taxon No . Col3r6 Vtpju e$ FRA P,J.£, 2 7 i ' -f. 28 29 30 31 12 33 34 35 36 37 38 39 40 41 42 43 44 # 45 46 47 48 49 50 51 52 S3 i Notes/Sketches/Additional Taxa 1^7 xj 7 ^4- M2. 07 5^ Pollution Index: PRA PTC No. 1 lk>. 2-4 x 1 = PRA PTC No. 2 3g,32. x 2 = PRA PTC No. 3 A iT. 44 X 3 = = ZZ^.ZQ Divided By 100 = l(p. z-4- + 6.4- + I3fe. 3z- Z. z-^z. Siltation Index: PRA Navicula sp . PRA Nitzschia sp . PRA Surirella sp . B.0(p 5*7 3 + tfajmaea/09-98/250 SITE SAMP TAXA SCL r I, 3co :hem DIATOM PROPORTIONAL COUNT Site-Sample No . ' Z=£ £ <0 Notebook No. [/ Water and Location i e. j-ov^ R.i^e.v (S- M i ^ k *>gt*-. 2-z-/ ( . Page No . ^3 , Sample Date °S> / os" / ffg Community, Collector/Agency £■■ fcS^clt^-off /^^^^P . Project Tmih — HUC / o o 3 o 2- c S" Reach No. . County Tg,-h^o Substrate NJ + *****»********* + ****** + + ***************** + ** + + **************■*******»****** Cells Counted 4 ©o Total Species 7 7 Diversity Index ■% . C> b 5" . Pollution Index z • 2?3 Similarity Index (compared to site-sample no Species Counted -S"Z. Siltation Index l2-.7o . Q8Z^ - e> z. ) S I ■ ^3 . No l 2 3 Taxon ^>^yivnx»J \/ali>c9 No CollG VUi^es PRA PTC HwkJI g^ H/Ull J 1 JLas~ 0.12- 2. 0- <.Uu ajL-As 75 ^11 Z. Qj nvCCA^>r.^f3^L>J.^ 7c £.75 CI. a^^fUA.c^pLa j a ^ 0,12- 0_ • Iwcn^AjJ-i <>-^ HOI 1. 1Z. -2l Ac>in- Z4,5 Ql eA-Trryy* . -T&r^u-e_ -f '7 - 15/ 2. 2. Rtv-tf-C^-C? 5? VJL*V^_s O.I z. 9 10 fa i&sa*fi o.zS ^a^cl^LA C2S ■Fr^^'UxU^ Va^jzl&u^UL 14s- laiz 2. 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 0.5b ^MiAfffa . CM>tclc5 -£- S^Kll— Jt^CH «J ^a •f- Z5- - ^» 3.2-5 Z. ^ . 4-a.yvr-fJl c-o. ¥- "§>\-&jAS.Sn\JU^J. ^>vUji-M-/ -P- £xipL?wej.s |tu^eJ[^, 0,1 2 Z. (S^zt^vu^3 U^rvi-o<^-loQ'U>i 0.5D ^^-txj (La , Bvsktligal^L. 0,50 CL^i cLf, vcXLa K^vLe q L^k^ -£- ^ Qfl. kWL CMXS ^ 5G_ 0.2> Z r^cJUldt.^ di*h >^C^JL^. jL ¥- '^2rUy\XAJLi\ £l_ _ -p- 37 Avv^U^U^iA^ ftl /LoL^L-^a— M 0.75- 2- S^kjlJ^. i^utoiA. 0.25" 39 Kl iUO-CC-CV U. Cua^A-c^ U^ 40 rwL*; .1-0 p luU^, 'eroKjiS^ 41 N ' V^ScJua^- t^rYV^fi^Linp- (, - K). <3-tiJ 1 todfcj^: J 0,1 2 42 K) , asrv^L^trCc^ 0.7S z. 43 kJ- djssj ! pa-j-r^. 7 o.81 44 ^ ■ 2>oJ-UA_s MM 1.37 45 Kj . Lx^-g-& / OS / Cj8 , Community. B Substrate rt Collector /Agency (2 . £>\Jalq H" / jjj t>EQ . Project T/v>bL- Hue IQQgg2.d)5" Reach No. . County TeJro-v\ *******+******+******+***+********+**++*+*+******+*+***********■ ********* Cells Counted Total Species. Pollution Index Diversity Index Similarity Index (compared to site-sample no.. Species Counted. Siltation Index No Taxon No. GeHrS Vetoes PRA PTC 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 typrvul U-o-nj^-tfL^ O. So KI -cx^ [a^ ^ frg-ovuL^- TVgLgi > L^LCdV^ (^-7VS>-UA^vl, j. O.I2 £- ^mulJ^^- (XjAjuc^j^ S>£l*dK^ 0,37 Ld o-ajzw^SlS k ,-> ! j-kt-ryvm-^ f&VLX f ■P- M rCMJ~lr.X/l. L, 4sVUZ-^ a. o. Z5" z. -p- (0 ^ .£- "^Scii^cg^' lMJr^Jvie_Ju_^_; -P- ■P(j2^c^r< I qu^«_> b5 iT2SC^lu^^ fejJ-gA^ 5 T^ C<~/KviM-(Lfl, (^JLJX-^«--%-uc>o. O.Z5 A-vK^U-o-ia Uk z. 2- M^l^a-*/-^.!^ VjgbodafcS *a < O.J 2- 9 O.Zi? 2. cH-iHX-^U^i 0^^i^~cJt-^pi<^etAi o.25~ Hi- j-g^rivLa- \A^ixaa\m I gj o-t^ O.I2. 2. Site-Sample No. 1 7-5 ^ 0\ Pa 4 ge / 4- Df i No. Taxon No. Gei4« \ZaAoas PRA PTC 27 £>~ctvy\ Y'lurr^JUAA-f— ti'VW-tcLu/TU m. 28 ' 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 -M 45 ft w 46 47 48 49 50 51 52 53 . Notes/Sketches/Additional Taxa : S-U-e^e- Ct^-aSL^2^_JL O2.^\o\<\<\ h^ k- • B^Jtls Sifc- L-cc^J-^ : T2.4M Ro4uJ 2.3 ^Cb u«4-. 4? 4# 4 2- kcwxj. |I2 £>4 5Z Pollution Index: PRA PTC No. 1 3-74 x 1 = PRA PTC No. 2 kS~.2-Q X 2 = PRA PTC No. 3 31 . OU x 3 = = ZZ7.3Z. Divided By 100 = 3.74- + i3o . 4o 9 5! 16 Z,Z73 Siltation Index: PRA Navicula sp. PRA Nitzschia sp . PRA Surirella sp . 6 .8S~ gV3£ + m \z c fc_s-f- A 4 I °\ & , Community "^ . Substrate. Collector/Agency C ■ <£s\ tjic-cjr / MD& . Project ~^.D <— HUC 1Qo3o 2-T^uJ^g^)M __________ _____ 17.25" _ 3_ 4_ _ 6_ 7 up LsCVU HA-n- &^^J-0JUL*As*vk_s 0.37 _£ p tX4xU-<^_Lx^V|A £>./Z /Wv^luvUC t KUZ/l/L&c/l g i 5 € ©.75 Arr^JoV-^L^^ f-A-JiJ. U<£m^^ ____! 5.6.2- £rp'4^^Lg_ _^-e_x_ CM .75" fV'C-Ci\ Vsi^ULA^ CCT^S.Wa-LjLsI*J> __i ■?- FT VfUA_C- v^tAA^ue. € Q.67 __ ______ 10 11 12 13 14 ______ 16 17 18 19 20 KiiA-p -xJLgH-gL^*, H_<^Cx<__U^g' OJZ. r^Uii __££ IKU. m \ .z> Q-- nO-cA-0 o-J)L^_ _____ Ao S~.oo _____ C- s. ^ _- mi 0.>O z. &h p U?vr_l<__. iy_[Cx3^ <9.£5 (__ A^JLt-<1 ba____ H U.ty^ HI Q.75- z_ R^^x^s^pA__^tx-x> £^/u>*iiT3^ _z 0. 87 imr^An^j. *. , KT^^t_A^Srw--^-^---rv-\ 0,(Z Qy- oCrv\^L. - p^e^(_^-JLuU-__. E CU^bd!^ £irv_ua_Ju^ mm .is Crrrv^jAX^Ty^Ji Ken. M^vvu-^r^-u-v^ m 2-b 21 22 23 _____ 25 26 J_l_ 'r^Jhm_fx II_____u___i O.5o 4-vi^Ii^l^Iju^^ a _; P-^La/-c-^__-*lj_^ l-7£T '^t^-JL ^ « 12. CM>w--_[r._ KMar<^ i_ o. IZ Ccc.c^pt-U..-- pUu^_|v-A c.^5 A^ia.r^) -S^JL^k ,1Xv-^M_M^ O.50 Site-Sample No. OS osr No 27 28 4- Page 2 of / N.C5^ 10 3.3) No. G&±±S.^cdoa-5 PRA PTC ^4- 3. co ■£- 29 N ■ ug-n^ Op ■37 30 NJ ■ v ej duA.r U2y\^^ O.Z5" (2> N •Mi it*—- e\U IZ.co 32 11 UMB o.z^r z. 33 N ■ C*-llyK> 3.oo 34 Al C#~<3 . 4-wJ-ei l^Lct^iO-jI'C^ — • Ml 1 -75- Z. 35 M jS^ aa /vUjf ^- 0.25" 37 M ■ "fevuJU-t^JLi ki- 47 M. -P Hi w C 2.SD 48 M ■ Vo-ld^i-u.^: c.37 z. 49 Ni. j£p_ P. So 5 0 M . ^Zxj-^5^ o.\Z 51 ^ ■ (jssULOAUl, o.37 52 M W~jc^rv^t> f ! ^~-cJL^?-z_Jl_ ^^//0/'^^ ^ ^ l^*Ix(.s Pollution Index: PRA PTC No. 1 By X 1 = X 2 = X 3 = 100 = PRA PTC No. 2 + PRA PTC No. 3 + = Divided Siltation Index: PRA Navicula sp . PRA Nitzschia sp . PRA Surirella sp . • Pk-^e 3 of Hannaea/09-98/250 SITE SAMP TAXA :hem DIATOM PROPORTIONAL COUNT Notebook No Site-Sample No Water and Location Tj*bL"g*3 &jq<^, (g> X"- /S" b^eL»^o tCcs,)- 4^t-^_ Sample Date OS / o4 / <="£ Page No. ^4- E> Community Collector/Agency C ■ t^i^i <=-o -H~ / rtb^c? , Project TMfr. Substrate N HUC Reach No . County. ♦ if************************************************************************ Cells Counted Total Species. Pollution Index Diversity Index Similarity Index (compared to site-sample no.. Species Counted. Siltation Index No 1 Taxon No. Gells~l4ic*?_s PRA PTC Fy-g»-^j i bz-^L& — Ca^> VxrrUL^S | S !L G. ZS 2_ 3_ 4_ 5_ 6_ 7 N^lj--gScioC* — (JLOU_U_yv-t O.Sp M, ■La^, ■fL^^CV^e UL^>U~*J.£ O.Z5 N^ ■ /V\J^A>CUXOucg4-^ £ O.Zb Ml4-^ciu*^^ *T*-^uXf^ O.S"o z fc^ p iA-K^wuj^-, £x-J(y~*\&-' fltyJ/ikiC^ 6f> O.IZ N < £A*Jui C-l KV< i e>.Z;f 12 13 N i -}- a S cXi^i_^ gyn^^_^-u^i*-Ia.^- O.'Z- 14 N O^L^O ii dZ 15 16 17 18 19 20 21 22 23 24 25 26 "S-r-^^u-yuJU— IpygJouss, o,37 Z. £it^^tr.Lc^ ^j? aJje 0,25" Z. n;1 4 S d^l<»^ iLOA- 0.2-5" ^fetdlCatAJfeJ o.Z5" \rv^)Um^, Kvum-b o.37 h-tpUvUui CbUm^fK £*=. 0,25" o,l2 — — ■ ■ — — ■ * ■ 9X^*Jjr-Su-*^ Qibb exudes 0,(Z- Sir**- Sample No. 0 3"\ 3 - 05~ 4 Page ^ 4- of A No. Taxon No. CollsV.UeS PRA PTC 27 ■ r. 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 0 45 46 47 48 49 50 51 52 53 i Notes/Sketches/Additional Taxa : S~L_J2_i_ £t*v>-a42cj *-cJP <=>Z-/lo[qc^ y ,. ^ ^^i_kLs ^>if& Uo<^J^rU • T2-SM R,oiu) ^a^fc^^^ 1.5" E>B/\ Pollution Index: PRA PTC No. 1 A A°\ X 1 = PRA PTC No. 2 46 : IB x 2 = PRA PTC No. 3 47-33 x 3 . = Z4z.84pivided By 100 = A 4l ^i* + . 5^ I4i to + PRA Nitzschia sp . ZfL 34- PRA Surirella sp . Q-37 • 5t- Sd r 75C Hannaea/09-98/250 Site-Sample No SITE SAMP IaXA CHEM DIATOM PROPORTIONAL COUNT 75 1 °f . Notebook No. lj_ Page No *-4- gjgg ^IJ-C-L ^g klew &rickaQ_ (u.S-^3 ^^.a-) Water and Location Sample Date ££ / °^ / ^& . Community &_ Collector /Agency C- . £Jy\d[c~g-\\~ /rfb£6( _ Project HUC loo 3c 2-g^" . Reach No. . County Substrate M 'mb. Te_k o^-\ *»****»**+*******+*******+*********+*+*******+*****+**+*********»********** Cells Counted A* Total Species. Az Species Counted 3& Diversity Index 3. 0^6 . Pollution Index 2 • S ' ^ . Siltation Index z-g-lj0. Similarity Index (compared to site-sample no.__£SO - C/5 ) ±>k? . 40* , No 2 3 Taxon No . eejAfl^gg1 PRA PTC /Iriiia^.'Wf, )-ni>vu|iS5(Kvm.^ Soo 37 5 A^y^pU^nM XJL^J^-C^\XW--*<~<)Ti be. MM 111 1.75" £ P . T^v^-a-^ /^-g^n^_>-g d».lZ 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 g; j> 1 -j^v^lcu- ^-ig-vXf q I. 12. ferl>g-cAu3i^ iku: ms-grT, mXi-*^ cn I. IZ Fya-g,i Lgyu-i^' U^fgrsfa/U^rvi [ jA d iaJ<7 > **- ) -£- t~- <^o-yT-S^-Txjq2^^.5 0.Z5" A, nj c^.C^e: ~\JLv^laJ~&\^ $- ArYY~yVjru^ i^-c-AXeA^sts £ 1 .00 A, y-e^jd-a-. © , Zb (^aLrv^tJ^. b0 Cg,ox^?-Kve^^_- ^.e^u^Xu^ 0,Z> (--^X^r^e_ju^ ULQ2A>Vl<3_U S (tJTM.il .) v\* O.ZS' Oj\1ch»*0-h 3 £>U^S ( g J f ciU_, J O.Zi' e^Aj'Ti 1 Ce gt 0.37 D t gLytrvm*-^ Tg^wA, 0."Zi> Site-Sample No o Page 2 of 2 €e4r3r3-Vtf./i.'es No. Taxon No PRA _PTC_ T 27 M. e^i CajJ c^ Z3 Z.87 (£1 M CA^p ■/-p-fe^xJd 0 13.75" 29 hj ■ vj l^yiur^- s.25 30 N >h*tx^i-KU^J . O. IS z. 36 M ■ WM0pUi ifr^- N . (lY I p^^oftAj 0,Z> 37 }-e^ a 0.75 38 N\ ■ XTa-dlo-Sg.^ o.iZ 39 Ki. ft a^ca — - 0.2i> 40 o,'Z z. 41 ^lf ^"pw* — ^0> 7.oo 2. 43 NJ • VgJdc&TrcgJT'i c>— 0,5fi Z_ 44 M • U^.b-<-A- O.JZ 45 M * 47 N ■ KwwS t* o.ZS z. 48 49 50 51 52 il '1 Notes/Sketches/Additional Taxa : ^"Uude*. o^\aXJi,r?<_JL OZ.J[oie\t^ ^ J^ . B«ckU •^ IS Lo^vo. Ill 35 1^ Pollution Index: PRA PTC No. 1 I. Z-g X 1 = f-Z-S" PRA PTC No . 2 4S-.37 x 2 ^\.^4 PRA PTC No. 3 ^2. IB X 3 = 158-34 _ Z5"1.53 Divided By 100 = 2-S/3~ Siltation Index: PRA Navicula sp . PRA Nitzschia sp . PRA Surirella sp . \<\. 73 + 3 87 + o . e> 7So Hannaea/09- 98/250 Site-Sample No SITE SAMP TAX A CHEM DIATOM PROPORTIONAL COUNT M5Z Notebook No. // Water and Location j g-jgvi ^o-C\- (g> "b ^-^f £r.'cU|g_ Page No. 25 Substrate M Sample Date OB I O 4 / ^8 , Community £> Collector/Agency C- ■ fjsVcj^ ££JL /^btS^? . Project ' Hfri— HUC I Q c 3 c Z- C S Reach No. . County Tm^i - Siltation Index n=» • 08. Similarity Index (compared to site-sample no . f ~2J=L * - o I ) _l_oj:_CLs£L • No. Taxon No. GeJrJrs^^eb PRA PTC ©- L^-|-vJylll C^ it HHA-<»VJtC- 6-3.IZ- (^yvw-ptMr^^J.^^^*— -^u.^niL^- ua: O.t.1 a M fiAAJL&AAJt^ ^S" O.kl K). £- 11 O.E5- CgJlo-^X^tf . vajl^IVx*.^ ii o.Z5 z Arv^pLwrL^ Ijl^Hjl c^-a Lu-^? MWflMnft 3. oo H 1. 00 (^u<^ 3 } iM-a^. <^ 1 O.IZ 9 10 11 12 13 ax IIOI I, oo 14 15 16 17 18 19 20 21 22 23 24 25 26 /L (/jui^jlMa, c^SSif^ofgJ i K ,' i 1.37 S\-vuusUrAJClsO *?- 0,(Z €Ipi \kjUvJL+^ •gnrULJt- CEJ1 I.SO nr^s)U Occc^ersxSli ^ ^ a^Ji^-c^^-Lu^iL o.fZ AvTA^k^tA n^-n ©, z^" ^l^Se^W-^ gj^k^JU^-. 7" o.37 Z. Nl^uxc^oLa, viyiciui^ Q. fa^n. cgr^SJ^-c-fp^-O ja 0,G>2- 0.37 bL ^f- O.I2- HffJUL I. 25" cunc U- o, (Z- 2 Site-Sample No. (*?5 Ol Page 2 of 2 No Taxon No aui&S 27 ($-p-^vp k<,-^ KAjt- S-^-c>tLg a£ o.ul 28 G^n^l^HlX Oc^ >H k-l T-P-1 n-QOg__ 29 4-u^ii^r.^-S -XA^l^— li/irA L £X JHL o.uz 30 (^yy^f. .urMA*-'* — ^A^> -^^u^ o.lZ z_ 31 ^i o.IZ 32 fH UI(a m-t/nv U*^ O.lZ 2 3 t^-W- tS I ^\ tvUD I N ja32 — o.ii 34 O .So 35 efKL \Ma^^ , C^^Sj fw-&vl3 O.fO 36 iL o.z-p 37 N -Uv. c^pioikjZU^& f~g — L^UrKJLMi^ L6w>-^-5 ©,IZ 42 OJZ 43 N £LO-CCiA_ LA_Lg^ 11 O.ZiT 44 >\o£ H^-* Hll^UM ,TT^->|K^U— II o,5o 45 aUaJ_j^_c ^ 1^*14^., J '^ u ^ ^^j^^ ^,f4£ <3-<<_ <_ ■y ^xcJ^-w^ lhu^, &(>«^-t- Pollution Index: PRA PTC No. 1 cs. l£ X 1 = U4\ H7 SZ -5^" Lovui .Ill |l SO 12.. I| t PRA PTC No. 2 g. As X 2 = Ik ■ *10 PRA PTC No. 3 ^1-^3 X 3 = ^74. Z^ = zq\ .3 I Divided By 100 = 2-^/ 3 Siltation Index: PRA Navicula sp . PRA Nitzscnia sp PRA Surirella sp Z*S5~ + _ 15. 0 c £ 20 H«Lanaea/09-98/250 SITE SAMP .AXA CHEM DIATOM PROPORTIONAL COUNT Site-Sample No . ° j ^ 2 OZ- Water and Location Ig-rgv} Ki . Notebook No . 1 1 Page No . Z^ 43 Sample Date og> / ° 4 / ?<9 Community Collector/Agency c ■ £^vclico if- /^fcegj^^ Project Substrate NJ >*lft HUC )PQ3C2-C5 Reach No County Ck, sk^-t -g-^A_ *** + **■ ■*********+**********+*****+***+***+*********+******************«*»* Cells Counted ^L CO Total Species. 4^ Species Counted 4~l Diversity Index 3. UBQ . Pollution Index z ■ S ~> 7 , Siltation Index 2.7. o^) , Similarity Index (compared to site- sample no . 1 "75 2- - O | ) 2 j , £?7 . No I] Taxon No. Cells^a-d^S' PRA PTC A ci\v\jLiAtU£/lA^vA_1£ 1 £ EM 1.75" i$T?^>^t^(ryi-e^L<»- 5-^-c re U -£- rra-^i 1 Lt/U,^ UJ_p f-zrs-f-a^^fr-to yj ■ cloJg t o^. -£- A\ ^Xa. C^r9 j . Uj£ avu-aJIl*7 a ir 0.(e>Z r<~a.e\ \ Lg^ca t KA*uy>^A_ O.Z5 Aro\/T Cec^JLUa Ij£asjjJJkl o.37 ^* Ju^x.Ll, -gi^q g-n.6 ^<«-^ c-^^oi?Jr^_ O.Zi>" l),j>Uvuu-^ (U^ffrf.. o.2S Z_ -hrou^LAA^^ ^a^e-UiA^sLi « I I.Z5" ^v^iv^Ce^i^L^ P^CUjcaJZa^ ©,Z5~ i|w|Lia yy\^rr\\TiAL0— &.l> &v*y« ^U-mg-m a. _ pU^tce-tAjy^ 0.37 Site-Sample No . g_3 7 - o g No 27 28 29 Taxon N guMx^ibx, M. {.*, {U*sK-£ fls\J-~0- rT~r (7 30 31 32 33 34 35 36 K Pc*-.' -•>-G^^l-g<_- U2^iv,v M ■ V1 g^LUT-p^- £j - U-B77M^vi-^M^ia^ M c*^ jg yp bL Sr^y^yVLgJfM-T^ - /vcck-^fx, „ NL flj, QrLu»-^-, . [0 . p*jLe^ N- luwiq CLAJ^UJU- M. ^A-cJ^ybspirxoa N ; V^Jt^i^lfa ^P'CM N. N- Nl. 4 UxjJii N • ^^u^{-goUj^»„ t^aj 52 .52. Page 2 of 2 No. eeJr4rsV^./u-e^ 1 iiOJt it ft (cIjux^ \ PRA 6.z5" 1.37 a.ZS" PTC X iET/ S3 o.5o /.tz £.56 ■ CO 0.75- o.SO o,Z3~ o.?7 &37 C37 2- Z_ z o.s~o \.oo -£- o . ?S o.ZS o.\ z o.so iz .is z Z- Notes/Sketches/Additional Taxa : ^Ul^. ^^L^ 62/1'/^ ^ k • £«*JU.U SiH«— ^oc^ofx^v, ; T-as-fO Roqt S^cjfucC-y lz.Cb4.c_ Uce-'h 4 7 ss ^^ kcnacj. no S0 5"? Pollution Index: PRA PTC No. 1 S.8 7 X 1 = g,g? PRA PTC No. 2 SO. CO x 2 = U\.Zo PRA PTC No 3 ("5.53 X 3 = I'io.S^ = Z-S?.(,L> Divided By 100 = Z,S77 Siltation Index: PRA Navicula sp . PRA Nitzschia sp . PRA Surirella sp . 13 .73 + 27,t