VOLUME

iATIONAL INSTITUTES OF HEALTH

v: OFFICE OF THE DIRECTOR

Animal Research Facilities Uling Static Microisolators

VENTILATION DESIGN HANDBOOK ON

National Institutes of Health

Ventilation Design Handbook on Animal Research Facilities

Using Static Microisolators

VOLUME II

Farhad Memarzadeh Principal Investigator

Division of Engineering Services Office of Research Services Office of the Director National Institutes of Health Bethesda, Maryland

September 1998

1

VOLUME II: TABLE OF CONTENTS

1. INTRODUCTION ii-i

1.1 Outline of CFD Baseline Model II - 1

1.2 Whole Room Configurations II - 8

2. SUMMARY OF RESULTS OF WHOLE ROOM RUNS ii 29

3. RANKINGS OF RESULTS OF WHOLE ROOM RUNS ii 37

3.1 Introduction II - 37

4. WHOLE ROOM SIMULATION DATABASE ii 55

4.1 Description of Simulation Data Entry 11-55

4.2 Simulation Data Entries 11-57

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3 0. 3

5"

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1. INTRODUCTION

This volume contains a database of the results of the computational fluid dynamics (CFD) simulations for the research done on the National Institutes of Health Ventilation Design Handbook on Animal Research Facilities using Static Microisolators.

Section 1 contains an overview of the basic room configuration studied and the variations considered. It is impossible to present all the data generated by the CFD simulations. The analysis of all the results produces an enormous quantity of data that would be difficult to present and interpret in its entirety. This volume contains summaries of the data in several forms.

Section 2 contains histograms of mean values for each room considered, as they apply to both the scientists’ breathing zone and the cages, for temperature, relative humidity, and CO2 and NH3 concentrations.

Section 3 contains rankings of the rooms based on different variables, for example, the selection of the room configuration with the lowest NH3 concentrations in the cages.

Section 4 contains a two page summary of each room simulation, including a description of the room, mean values and standard deviations for all parameters, and histograms showing the distribution of values in the cages.

1.1 Outline of CFD Baseline Model

A typical animal research facility of average size, air change rate, rack layout, mouse population, pressurization, and so on, was modeled as the baseline model for the CFD simulations. The general features of the room are shown in figure 1.01 and are listed below.

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Figure 1.01 Overall Layout of Animal Room Basecase

Description in brief

The general features of the basecase room model were:

Room-. 6.10m x 3.60m x 4.22m (20’ 0” x 12’ 0” x 9’ 0”) Door in short wall Sink in comer

Laminar flow change station 5 racks

Cages: Microisolator (with filter top) mouse cage

5 mice per cage

Rack: Static system

6 shelves per rack

7 cages per shelf (42 cages per rack)

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Supply. 2 radial supplies each providing 270cfm (0. 13m^/s) for a total of 15 ACH

Supply discharge temperature of 18.8 °C (66 °F), set such that the exhaust air temperature was 22.2 °C (72.0 °F)

61 percent relative humidity (to provide 50 percent RH at 22.2 °C (72.0 °F) Exhausts’. 2 ceiling level exhausts removing 220cfm (0.1 mVs) each Makeup Air. lOOcfm (0.047m /s) coming from around the door Overall Geometry

In the majority of the cases considered, the animal room occupied a floor area of 6.10m (20’ 0”) X 3.66m (12’ 0”). In some cases that were considered, the width of the room was increased from 3.66m to 4.22m (14’ 0”). The ceiling height in all cases was 2.74m (9’ 0”). There was only one door in the room, mounted centrally on one of the short walls.

In all the displacement ventilation systems considered in this project, air was introduced through ceiling mounted diffusers. All devices were mounted flush with the ceiling surface; there was no ductwork present within the upper room volume. The various diffuser types considered in this project were all modeled using a combination of several boundary conditions that were validated previously (see volume I, section 4.2.2). All the air exited through general exhausts. The number and locations of the exhausts were varied. In line with common practice, there was an imbalance between the amount of air supplied to the room and exhausted from the room. This leads to an overall pressurization of the room relative to the rooms or corridors surrounding the room. The relative level of pressurization was a parameter considered in this study. The makeup air to compensate for the supply/exhaust imbalance was allowed to enter or leave the room through 6.35e-3m (0.25”) gaps on three sides of the door.

The rooms considered in this project all contained five animal cage racks. The rooms also contained one of two alternative design change stations. A fuller description of these items is given below. The only other item within the room was a sink of 0.61m (24”) x 0.61m (24”) x 0.81m (32”) that was situated in one of the comers to the room.

In all cases, the room was considered under dark period conditions, i.e., the lights were off and produced no additional heat load to the room. Dark period conditions were chosen because early experimental work had indicated that heat, CO2, and NH3 generation were higher in the dark period compared with periods in which the lights were on.

Rack Model

The overall dimensions of the rack were 1.52m (60”) x 0.61m (24”) x 1.83m (72”) high, as shown in figure 1.02. There were six shelves in the rack. The spacing of the shelves was 0.32m (12.75”) from top surface to top surface, with the lowest shelf at a height of 0.21m (8.25”) above the floor. The shelves were modeled as thin rectangular blocks. Details, such as the connecting ties between the shelves and the rollers on which the racks move, were not modeled because their effect on the overall flow field and gas concentration distributions was considered insignificant.

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Figure 1.02 CFD Model of Cage Rack

Located on the shelves of the racks were representations of the animal cages, shown in figure 1.02. The dimensions of the cage were 0.27m (10.7”) x 0.16m (6.38”) x 0.21m (8.39”) high, which maintained the volume of the original cage that had sloped sides. The sides of the cage were modeled as thin plates, with the thickness and conductivity of the plates set to those of the physical cage polycarbonate. The water bottle and food normally found in a cage were modeled as a single block in order to reduce the computational overhead. The volume of the block was the same as that of the bottle and food combined. The bedding of the cage was included as a rectangular block of 0.27m (10.7”) x 0.16m (6.38”) x 1.27e-2m (0.5”).

The mice were modeled as a block of 0.11m (4.25”) x 8.57e-2m (3.38”) x 0.22m (0.88”), the same representation that was used in the experimental cage wind tunnel tests (see volume I, section 4.1.1). This simulated the effect of ‘huddling’ by the mice. The surface temperature of the block was fixed at 30.0 °C (86.0 °F), which is agreed to be a typical mouse body surface temperature.

Surrounding this block, a source of CO2 was defined at 2.12e-7 kg/s (0.76g/hr), which was based on the generation rate indicated for the dark period in the early tests on the effect of the

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photoperiod on the mice. The supply air was assumed to have a zero concentration of CO2. This source allowed the additional concentration of CO2 in the air to be calculated in the simulation. It also allowed the concentration of NH3, among other things, to be calculated by scaling even though it has a different molecular weight from both air and CO2. This was possible as the magnitude of the source was very small and the resulting concentrations were so low as to have a negligible effect on the density of the mixture of air, CO2, and NH3. In effect, the CO2 and NH3 are intimately mixed with, and flow with, the air.

Experimental data later showed the generation rate of CO2 was actually higher than the source used in the CFD simulations at 0.90 g/hr/lOOg mouse body weight. This means the concentration of CO2 in the room and cages was derived from the simulated concentration multiplied by a scaling factor (0.90/0.76). The concentrations of NH3 in both the cages and the room were also derived by scaling the concentration with a factor specified in the post-processing of the data. This factor was assumed to vary according to the number of days that passed since the bedding in the cage was changed, along with the average relative humidity in the cages. See volume I, section 4.2. 1.2 for the experimental determination of the factors.

Background levels of CO2 and NH3 were assumed to be zero. This means that all values quoted in the CFD section of the report are relative to the background level. If an absolute value for CO2 is required, an additional amount in the range of SOOppm to 700ppm should be added for most locations.

The remaining cage boundary conditions are associated with the transfer mechanisms for air/gases to enter/leave the cage. The cracks at the side of the cage were modeled as 6.35e-3m (0.25”) high planar resistances, with the loss coefficient for these resistances having been determined from the cage wind tunnel CFD simulations (see volume I section 4.2. 1.2). The top of the cage, which was filtered, was defined as a combination of a planar resistance and a planar source. The determination of the loss coefficient for the resistance, and the coefficient for the source has been outlined (see volume I, section 4.2. 1.1).

The spacing of the cages on the shelves was dependent on whether the racks were single density (7 cages per shelf), or double density (14 cages per shelf). In the single density cases, the cages were centrally located in the short dimension, and equally spaced in the long dimension. The spacing was 4.88e-2m (1.92”) from comer of cage to comer of adjacent cage. In the double density racks, the cages were equally spaced in both the long and short dimensions. The spacing was 2.20e-2m (0.87”) and 4.88e-2m (1.92”), respectively.

Change Station Model

Two alternative change stations were considered in this project. Both stations were constmcted primarily from rectangular blocks and triangular prisms. The internal stmcture and flow field were of no concern in this project. It was only the effect of the station on the room airflow that is of importance.

The first design is shown in figure 1.03. The station had overall dimensions of 1.32m (52”) x 0.86m (34”) x 1.83m (72”). This design was effectively passive in terms of direct flow field interaction. In particular, the station internally recirculated a flow of 350 cfm (1.65e-l m^ /s).

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with only 10 percent leakage defined at the sash opening. The makeup air intake for this leakage was mounted at the side of the station. The station dissipated heat that was expected to affect the room’s overall flow field. In particular, the station contributed a load of 720W to the room. This heat was mostly confined to the lower portion of the station where the motor was located.

Figure 1.03 Original Change Station Design

Due to concerns raised over the relative passiveness of the first station design, a second design was considered, as is displayed in figure 1 .04. The station had overall dimensions of 1 .50m (59”) X 0.86m (34”) x 1.93m (76”) high. This station also recirculated air, at 300 cfm (1.42e-l m^ /s), but discharged a much higher percentage than the first design. In particular, 200 cfm (9.44e-2 m^/ s) was discharged through grilles at the top of the station. The air makeup to compensate for this discharge was mounted at the front sill at the opening to the station. The station dissipated 660W, and this heat was considered to be added to the air discharge at the top of the station.

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Figure 1.04 Alternative Change Station Design

Modeling Assumptions

• Solar load was not modeled through the walls of the room.

• The floor, ceiling, and walls were assumed to have no heat transfer, i.e., the surrounding areas were assumed to be at the same temperatures.

• All surfaces were treated as smooth when calculating surface friction.

• The sink in the animal room was modeled as a single rectangular block. The recess formed by the shape of the sink was not modeled because the effect of the recess would be negligible on the overall flow field within the room.

• No lighting was assumed in the animal room simulations. The reason for this was that higher CO2 and NH3 emissions occur during dark (scotophase) conditions (see volume I, section 4. 1.2.8. 1 and appendix I, sections 3.4. 1.9 and 3.4.1.10).

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• The animal room was intended to be kept at 22.2 °C (72.0 °F), with temperature control performed on the exhaust air temperature.

• No leakage occurred into or out of the animal room other than that specified though the cracks around the door.

• Air density variations due to temperature were negligible. Density variation was therefore ignored in all terms apart from in the momentum term for the vertical velocity component. This is known as the Boussinesq approximation.

• The levels of CO2 and NH3 were so diluted in the whole room simulations, even at their source, that the variation of the mixture density due to differing molecular weights was negligible.

1.2 Whole Room Configurations

To investigate a range of parameters, the basic model described in section 1.1 was modified.

Wherever possible, only one parameter was varied at a time to fully assess the effect.

The list of room runs as they were considered is given in table 1.01. Parameters considered in

this report are as follows:

• Supply Diffuser Type: Three different diffuser types were considered in this project: radial diffusers, low induction diffusers, and slot diffusers. While all these diffuser types are ceiling mounted diffusers, the flow patterns resulting from them are fundamentally different. See volume I, section 4.2.2 and figures 1.05 to 1.07).

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Figure 1.05 Radial Dijfuser

Figure 1.06 Slot Dijfuser

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Figure 1.07 Low Induction Dijfuser

• Exhaust Location and Number. Four different exhaust locations were considered: ceiling; high level, low level on the long walls, and low level on the door wall. The number of exhausts used in each of these locations was also considered as a parameter. See figures 1 .08 to 1.11.

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Figure 1.08 Ceiling Level Exhausts

Figure 1.09 High Level Exhausts

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Figure 1.10 Low Level Exhausts

Figure 1.11 Low Level Exhausts on the Door Wall

• Room Air Change Rate: addition to the basecase ACH of 15, the following three other

ACH values were considered; 5, 10, and 20 ACH.

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• Change Station Design and Status: Two different change station designs were considered, as outlined above. The two designs were intended to present both a passive and intrusive influence on the room volume. In the case of the former design, the station was also considered switched off.

• Pressurization of Room Relative to Corridor: The pressurization of a room is dependent on the difference between the supply and exhaust flow rates. A higher exhaust rate than supply leads to negative pressurization of the room; hence, makeup air is supplied to the room via available transfer mechanisms, namely door cracks. In this project, the amount of makeup air allowed through the door cracks was varied between 100 cfm (4.72e-l mVs) into the room to 100 cfm (4.72e-l mVs) out of the room.

• Orientation of Cage Racks in Room: The racks were considered both parallel to the long walls, and perpendicular to them. See figures 1.12 and 1.13

Figure 1.12 Racks Parallel to Side Walls

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Figure 1.13 Racks Perpendicular to Side Walls

• Side Cracks of Cage Sealed Instead of Open: A series of cases was considered in which the side cracks of the cage were sealed, leaving the cage filter top as the only transfer mechanism. The main objective was to establish the relative increase in cage NH3 and CO2 levels when the secondary transfer mechanism was removed.

• Density of Cages: The number of cages per rack was considered as a parameter. In addition to the basecase value of 42 cages per rack, a double density 84 cages per rack was also considered, as was a reduced density of 28 cages per rack. See figures 1.14 and 1.15.

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Figure 1.14 Double Density Rack

Figure 1.15 Reduced Density Rack

• Location of Change Station: The location of the change station was swapped with each of the racks in the room. See figures 1.16 to 1.20.

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Figure 1.17 Change Station Swapped with Rack 2

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Figure 1.18 Change Station Swapped with Rack 3

Figure 1.19 Change Station Swapped with Rack 4

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Figure 1.20 Change Station swapped with Rack 5

• Rack Grouping: In some series, the racks were considered to be along one wall only, rather than spread out through the room. See figure 1.21.

Figure 1.21 Racks on One Wall Only

• Room Width: Three runs were performed with the room width increased to 4.26m (14’ 0”).

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• Supply Air Temperature: In a few runs the supply air temperature was raised to 22.2 °C (72.0 °F), at 49 percent RH which would cause the room and exhaust temperatures to be higher than the design value of the baseline case.

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Table 1.01 List of All Whole Room Runs

Case Name	Supply Diffuser Type	Exhaust Location and Number	Change Station (Design/ Status)	Rack orientation	Rack density	Pressure of Room to Corridor	Supply Temperature °C (“F)	Supply ACH
Basecase	Radial	Ceiling (x2)	Thoren/ ON	Parallel	Single	Neg. lOOcfm	18.8 (65.8)	15
Case 02	Radial	High (x4)	Thoren/ ON	Parallel	Single	Neg, lOOcfm	18.8(65.8)	15
Case 03	Radial	Low (x4)	Thoren/ ON	Parallel	Single	Neg. lOOcfm	18.8(65.8)	15
Case 04	Slot	Ceiling (x2)	Thoren/ ON	Parallel	Single	Neg. lOOcfm	18.8(65.8)	15
Case 05	Slot	High (x4)	Thoren/ ON	Parallel	Single	Neg. lOOcfm	18.8(65.8)	15
Case 06	Slot	Low (x4)	Thoren/ ON	Parallel	Single	Neg. lOOcfm	18.8(65.8)	15
Case 07	Low Ind	Ceiling (x2)	Thoren/ ON	Parallel	Single	Neg. lOOcfm	18.8(65.8)	15
Case 08	Low Ind	High (x4)	Thoren/ ON	Parallel	Single	Neg. lOOcfm	18.8(65.8)	15
Case 09	Low Ind	Low (x4)	Thoren/ ON	Parallel	Single	Neg. lOOcfm	18.8(65.8)	15
Case 10	Radial	Ceiling (x2)	Thoren/ OFF	Parallel	Single	Neg. lOOcfm	20.7 (69.3)	15
Case 11	Radial	Low (x4)	Thoren/ OFF	Parallel	Single	Neg. lOOcfm	20.7 (69.3)	15
Case 12	Slot	Ceiling (x2)	Thoren/ OFF	Parallel	Single	Neg. lOOcfm	20.7 (69.3)	15
Case 13	Slot	Low (x4)	Thoren/ OFF	Parallel	Single	Neg. lOOcfm	20.7 (69.3)	15
Case 14	Low Ind	Ceiling (x2)	Thoren/ OFF	Parallel	Single	Neg. lOOcfm	20.7 (69.3)	15
Case 15	Low Ind	Low (x4)	Thoren/ OFF	Parallel	Single	Neg. lOOcfm	20.7 (69.3)	15
Case 16	Radial	Ceiling (x2)	Thoren/ ON	Parallel	Single	Pos. lOOcfm	18.8(65.8)	15
Case 17	Slot	Ceiling (x2)	Thoren/ ON	Parallel	Single	Pos. lOOcfm	18.8(65.8)	15
Case 18	Low Ind	Ceiling (x2)	Thoren/ ON	Parallel	Single	Pos. lOOcfm	18.8(65.8)	15
Case 19	Radial	Ceiling (x2)	Thoren/ ON	Perpendicular	Single	Neg. lOOcfm	18.8(65.8)	15
Case 20	Slot	Ceiling (x2)	Thoren/ ON	Perpendicular	Single	Neg. lOOcfm	18.8(65.8)	15
Case 21	Low Ind	Ceiling (x2)	Thoren/ ON	Perpendicular	Single	Neg. lOOcfm	18.8(65.8)	15
Case 22	Radial	Low (x4)	Thoren/ ON	Perpendicular	Single	Neg. lOOcfm	18.8(65.8)	15
Case 23	Slot	Low (x4)	Thoren/ ON	Perpendicular	Single	Neg. lOOcfm	18.8(65.8)	15
Case 24	Low Ind	Low (x4)	Thoren/ ON	Perpendicular	Single	Neg. lOOcfm	18.8(65.8)	15
Case 25 *	Radial	Ceiling (x2)	Thoren/ ON	Parallel	Single	Neg. lOOcfm	18.8(65.8)	15
Case 26 *	Slot	Ceiling (x2)	Thoren/ ON	Parallel	Single	Neg. lOOcfm	18.8 (65.8)	15
Case 27 *	Low Ind	Ceiling (x2)	Thoren/ ON	Parallel	Single	Neg. KXlcfm	18.8 (65.8)	15
Case 28	Low Ind	Low (x4)	Thoren/ ON	Parallel	Single	Neg. lOOcfm	16.8(62.2)	10
Case 29	Low Ind	Low (x4)	Thoren/ ON	Parallel	Single	Neg. lOOcfm	11.0(51.8)	5
Case 30	Low Ind	Low (x4)	Thoren/ ON	Parallel	Single	Neg. KXlcfm	19.8(67.6)	20
Case 31	Radial	Low (x4)	Thoren/ ON	Perpendicular	Double	Neg. lOOcfm	17.5 (63.5)	15
Case 32	Slot	Low (x4)	Thoren/ ON	Perpendicular	Double	Neg. lOOcfm	17.5(63.5)	15
Case 33	Low Ind	Low (x4)	Thoren/ ON	Perpendicular	Double	Neg. KXlcfm	17.5(63.5)	15
Case 34	Radial	Ceiling (x2)	Thoren/ ON	Perpendicular	Double	Neg. lOOcfm	17.5(63.5)	15
Case 35	Slot	Ceiling (x2)	Thoren/ ON	Perpendicular	Double	Neg. lOOcfm	17.5(63.5)	15
Case 36	Low Ind	Ceiling (x2)	Thoren/ ON	Perpendicular	Double	Neg. lOOcfm	17.5 (63.5)	15
Case 37	Radial	Ceiling (xl) / Low (x4) (Mass flow in 50/30 split)	Thoren/ ON	Parallel	Single	Neg. lOOcfm	18.8(65.8)	15
Case 38	Slot	Ceiling (xl) / Low (x4) (Mass flow in 50/50 split)	Thoren/ ON	Parallel	Single	Neg. lOOcfm	18.8(65.8)	15
Case 39	Low Ind	Ceiling (xl) / Low (x4) (Mass flow in 50/50 split)	Thoren/ ON	Parallel	Single	Neg. lOOcfm	18.8(65.8)	15
Case 40	Radial	Ceiling (x4)	Thoren/ ON	Parallel	Single	Neg. lOOcfm	18.8(65.8)	15
Case 41	Slot	Ceiling (x4)	Thoren/ ON	Parallel	Single	Neg. lOOcfm	18.8(65.8)	15
Case 42	Low Ind	Ceiling (x4)	Thoren/ ON	Parallel	Single	Neg. lOOcfm	18.8(65.8)	15

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Case Name	Supply Diffuser T)rpe	Exhaust Location and Number	Change Station (Design/ Status)	Rack orientation	Rack density	Pressure of Room to Corridor	Supply Temperature °C (T)	Supply ACH
Case 43	Lx)w Ind (rotated by 90°)	Ceiling (x2)	Thoren/ ON	Parallel	Single	Neg. lOOcfm	18,8 (6S.8)	15
Case 44	Radial (rotated by 90°)	Ceiling (x2)	Tboren/ ON	Parallel	Single	Neg. lOOcfm	18.8 (6S.8)	15
Case 45	Radial	Ceiling (x2)	Thoren/ ON	Parallel	Single	Neg, SOcfm	18.8 (6S.8)	15
Case 46	Radial	Ceiling (x2)	Thoren/ ON	Parallel	Single	Neutral	18.8 (6S.8)	15
Case 47	Radial	Ceiling (x2)	Thoren/ ON	Parallel	Single	Pos. SOcfm	18.8 (6S.8)	15
Case 48	Radial	Ceiling (x2)	Thoren/ ON (swapped with rackl )	Parallel	Single	Neg. lOOcfm	18.8 (6S.8)	15
Case 49	Radial	Ceiling (x2)	Thoren/ ON (swapped with rack2)	Parallel	Single	Neg. lOOcfm	18.8 (6S.8)	15
Case 50	Radial	Ceiling (x2)	Thoren/ ON (swapped with rack3)	Parallel	Single	Neg. lOOcfm	18.8 (6S.8)	15
Case 51	Radial	Ceiling (x2)	Thoren/ ON (swapped with rack4)	Parallel	Single	Neg. lOOcfm	18.8 (6S.8)	15
Case 52	Radial	Ceiling (x2)	Thoren/ ON (swapped with racks)	Parallel	Single	Neg, lOOcfm	18.8 (6S.8)	15
Case 53	Radial	Ceiling (x2)	Lab. Prod. / ON	Parallel	Single	Neg. lOOcfm	18.8 (6S.8)	15
Case 54	Slot	Ceiling (x2)	Lab. Prod. / ON	Parallel	Single	Neg. lOOcfm	18.8 (6S.8)	15
Case 55	Low Ind	Ceiling (x2)	Lab. Prod. / ON	Parallel	Single	Neg. lOOcfm	18.8 (6S.8)	15
Case 56	Radial	High (x4)	Lab. Prod. / ON	Parallel	Single	Neg. lOOcfm	18.8 (6S.8)	15
Case 57	Radial	Low (x4)	Lab. Prod. / ON	Parallel	Single	Neg. lOOcfm	18.8 (6S.8)	15
Case 58	Slot	High (x4)	Lab. Prod. / ON	Parallel	Single	Neg. lOOcfm	18.8 (6S.8)	15
Case 59	Slot	Low (x4)	Lab. Prod. / ON	Parallel	Single	Neg. lOOcfm	18.8 (6S.8)	15
Case 60	Low Ind	High (x4)	Lab. Ihod. / ON	Parallel	Single	Neg. lOOcfm	18.8(65.8)	15
Case 61	Low Ind	Low (x4)	Lab. Prod. / ON	Parallel	Single	Neg. lOOcfm	18.8(65.8)	15
Case 62	Low Ind	Low (x4)	Thoren/ ON	Perpendicular	Double	Neg. lOOcfm	6.6 (43.9)	5
Case 63	Low Ind	Low (x4)	Thoren/ ON	Perpendicular	Double	Neg. lOOcfm	14.8(58.6)	10
Case 64	Low Ind	Low (x4)	Thoren/ ON	Perpendicular	Double	Neg. lOOcfm	18.9 (66.0)	20
Case 65	Low Ind	Low (x4)	Lab. Prod. / ON	Perjjendicular	Double	Neg. lOOcfm	17.5 (63.5)	15
Case 66	Low Ind	Low (x4)	Lab. Prod. / ON	Perpendicular	Double	Neg. lOOcfm	6.6 (43.9)	5
Case 67	Low Ind	Low (x4)	Lab. Prod. / ON	Perpendicular	Double	Neg. lOOcfm	14.8(58.6)	10
Case 68	Low Ind	Low (x4)	Lab. Prod. / ON	Perpendicular	Double	Neg. lOOcfm	18.9 (66.0)	20
Case 69	Radial	High (x4) / Low (x4) (Mass flow split evenly amongst exhausts)	Thoren/ ON	Parallel	Single	Neg. l(X)cfm	18.8(65.8)	15

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Case Name	Supply Diffuser Type	Exhaust Location and Number	Change Station (Design/ Status)	Rack orientation	Rack density	Pressure of Room to Corridor	Supply Temperature °C(T)	Supply ACH
Case 70	Slot	High (x4) / Low (x4) (Mass flow split evenly amongst exhausts)	Thoren/ ON	Parallel	Single	Neg. lOOcfm	18.8(65.8)	15
Case 71	Low Ind	High (x4) / Low (x4) (Mass flow split evenly amongst exhausts)	Thoren/ ON	Parallel	Single	Neg. KXlcfrn	18.8(65.8)	15
Case 72	Radial	High (x4) / Low (x2) (Mass flow split evenly amongst exhausts)	Thoren/ ON	Parallel	Single	Neg. lOOcfm	18.8(65.8)	15
Case 73	Slot	High (x4) / Low (x2) (Mass flow split evenly amongst exhausts)	Thoren/ ON	Parallel	Single	Neg. lOOcfm	18.8(65.8)	15
Case 74	Low Ind	High (x4) / Low (x2) (Mass flow split evenly amongst exhausts)	Thoren/ ON	Parallel	Single	Neg. lOOcfm	18.8(65.8)	15
Case 75	Radial	Ceiling (xl) / Low (x4) (Mass flow in 50/50 split)	Lab. Prod. / ON	Parallel	Single	Neg. lOOcfm	18.8(65.8)	15
Case 76 **	Radial	Ceiling (x2)	Thoren/ ON	Parallel	Single	Neg. lOOcfm	19.2 (66.6)	15
Case 77 **	Slot	Ceiling (x2)	Thoren/ ON	Parallel	Single	Neg. lOOcfm	19.2(66.5)	15
Case 78	Low Ind	Ceiling (x2)	Thoren/ ON	Parallel	Single	Neg. lOOcfm	19.2(66.6)	15
Case 79	Radial	2 Door exhausts	Thoren/ ON	Parallel	Single	Neg. lOOcfm	18.8(65.8)	15
Case 80	Slot	2 Door exhausts	Thoren/ ON	Parallel	Single	Neg. lOOcfm	18.8(65.8)	15
Case 81	Low Ind	2 Door exhausts	Tlioren/ ON	Parallel	Single	Neg. lOOcfm	18.8(65.8)	15
Case 82	Radial	2 Door exhausts	Thoren/ ON	Parallel	Single	Neg. lOOcfm	18.8(65.8)	15
Case 83	Slot	2 Door exhausts	Thoren/ ON	Perpendicular	Double	Neg. lOOcfm	17.5 (63.5)	15
Case 84	Low Ind	2 Door exhausts	Thoren/ ON	Perpendicular	Double	Neg. lOOcfm	17.5 (63.5)	15
Case 85	Radial	Low (x4)	Lab. Prod. / ON	Perpendicular	Double	Neg. lOOcfm	17.5 (63.5)	15
Case 86	Slot	Low (x4)	Lab. Prod. / ON	Perpendicular	Double	Neg. lOOcfm	17.5(63,5)	15
Case 87	Radial	Low (x4)	Thoren/ ON	Parallel	Reduced	Neg. lOOcfm	19.2(66.6)	15
Case 88	Slot	Low (x4)	Thoren/ ON	Parallel	Reduced	Neg. lOOcfm	19.2(66.6)	15
Case 89	Low Ind	Low (x4)	Thoren/ ON	Parallel	Reduced	Neg, lOOcfm	19.2(66.6)	15
Case 90	Radial	Ceiling (x2)	Thoren/ ON	Perpendicular all 5 on 1 wall	Single	Neg. lOOcfm	18.8(65.8)	15
Case 91	Slot	Ceiling (x2)	Thoren/ ON	Perpendicular all 5 on 1 wall	Single	Neg. lOOcfm	18.8(65.8)	15
Case 92	Low Ind	Ceiling (x2)	Thoren/ ON	Perpendicular all 5 on 1 wall	Single	Neg. lOOcfm	18.8(65.8)	15

Volume II - Section I - Introduction

Page n - 23

Case Name	Supply Diffuser Type	Exhaust Location and Number	Change Station (Design/ Status)	Rack orientation	Rack density	Pressure of Room to Corridor	Supply Temperature °C(“F)	Supply ACH
Case 93	Radial	Ceiling (x2)	Thoren/ ON	Perpendicular all 5 on 1 wall	Double	Neg. lOOcfm	17.5(63.5)	15
Case 94	Slot	Ceiling (x2)	Thoren/ ON	Perpendicular all 5 on 1 wall	Double	Neg. lOOcfm	17.5(63.5)	15
Case 95	Lx)w Ind	Ceiling (x2)	Thoren/ ON	Perpendicular all 5 on 1 wall	Double	Neg, lOOcfm	17.5 (63.5)	15
Case 96	Radial	Ceiling (x2)	Lab, Prod. / ON	Perpendicular all 5 on 1 wall	Double	Neg. lOOcfm	17.5 (63.5)	15
Case 97	Slot	Ceiling (x2)	Lab. Prod. / ON	Perpendicular all 5 on 1 wall	Double	Neg. lOOcfm	17.5(63.5)	15
Case 98	Low Ind	Ceiling (x2)	Lab. Prod. / ON	Perpendicular all 5 on 1 wall	Double	Neg. lOOcfm	17.5(63.5)	15
Case 99 ***	Radial	Ceiling (x2)	Thoren/ ON	Parallel	Single	Neg. l(X)cfm	22.2 (72,0)	15
Case 100 ***	Radial	Low (x4)	Thoren/ ON	Parallel	Single	Neg. lOOcfm	22.2 (72.0)	15
Case 101 ***	Low Ind	Low (x4)	Lab. Prod. / ON	Perjjendicular	Double	Neg. lOOcfm	22.2 (72.0)	5

* Sealed cages instead of open cages

** Room 4.26m (14’ 0”) wide instead of 3.66m (12’ 0”)

*** Supply air temperature fixed at 22.2 °C (72.0 °F). In all other cases, the exhaust temperature was set to be 22.2 °C (72.0 °F) by setting the supply air temperature appropriately.

Volume 11-24

Ventilation Design Handbook on Animal Research Facilities Using Static Microisolators

Table 1.02 Cases Cross-Referenced Against Supply Diffuser Type

Supply Diffuser Type	Case Number
Radial	Basecase, 02-03, 10-11, 16, 19, 22, 25, 31, 34, 37, 40, 44*, 45-53, 56-57, 69, 72, 75- 76, 79, 82, 85, 87, 90, 93, 96, 99-100
Slot	04-06,12-13, 17, 20, 23, 26, 32, 35, 38, 41, 54, 58-59, 70, 73, 77, 80, 83, 86, 88, 91, 94, 97
Low Induction	07-09, 14-15, 18, 21, 24, 27-30, 33, 36, 39, 42, 43*, 55, 60-68, 71, 74, 78, 81, 84, 89, 92, 95,98, 101

* Indicates that diffuser rotated by 90°

Table 1.03 Cases Cross-Referenced Against Exhaust Location and Number

Exhaust Location and Number	Case Number
Ceiling (x2)	Basecase, 04, 07, 10, 12, 14, 16-21, 25-27, 34-36, 43-55, 76-78, 90-99
High (x4)	02, 05, 08, 56, 58, 60
Low (x4)	03, 06, 09, 1 1, 13, 15, 22-24, 28-33, 57, 59, 61-68, 85-89, 100-101
Ceiling (xl) / Low (x4) (Mass flow in 50/50 split)	37-39, 75
Ceiling (x4)	40-42
High (x4)/ Low (x4) (Mass flow split evenly amongst exhausts)	69-71
High (x4)/ Low (x2) (Mass flow split evenly amongst exhausts)	72-74
2 Door Exhausts	79-84

Volume II - Section I - Introduction

Page II - 25

Table 1.04 Cases Cross-Referenced Against Change Station Design and Status

Change Station	Case Number
ON*	Basecase-09, 16-101
OFF*	10-15
Thoren Design	Basecase -52**, 62-64, 69-74, 76-84, 87- 95,99-100
Laboratory Products Design	53-61, 65-68, 75, 85-86, 96-98, 101

^Change in status was only considered for Thoren design change station. **Cases 10 to 15 had Thoren design change station switched off.

Table 1.05 Cases Cross-Referenced Against Pressurization of Room Relative to Corridor

Pressurization of Room to Corridor	Case Number
Neg. 100 cfm	basecase -15, 19-44,48-101
Pos. 100 cfm	16-18
Neg. 50cfm	45
Neutral	46
Pos. 50 cfm	47

Table 1.06 Cases Cross-Referenced Against Pressurization of Room Relative to Corridor

Rack Orientation	Case Number
Parallel	basecase - 18, 25-30, 37-61***, 69-82, 87- 89, 99-100
Perpendicular	19-24, 31-36, 62-68, 83-86,101
Perpendicular All 5 on 1 Wall	90-98

*** Cases 48 to 52 had change station swapped with each of five racks.

Table 1.07 Cases Cross-Referenced Against Status of Cage Side Cracks

Status of Cage Side Cracks	Case Number
Open	basecase - 24, 28-101
Sealed	25-27

Volume II - 26

Ventilation Design Handbook on Animal Research Facilities Using Static Microisolators

Table 1.08 Cases Cross-Referenced Against Density of Cages in Rack

Density of Cages in Rack	Case Number
Single	basecase - 30, 37-61, 69-82, 90-92, 99-100
Double	31-36, 62-68, 83-86, 93-98, 101
Reduced	87-89

Table 1.09 Cases Cross-Referenced Against Room Air Change Rate

Room Air Change Rate (ACH)	Case Number
5	29, 62, 66, 101
10	28, 63, 67
15	basecase - 27, 31-61, 65, 69-100
20	30, 64, 68

Table 1.10 Cases Cross-referenced Against Room Width

Room Width (ft)	Case Number
12	basecase -75, 79-101
14	76-78

Table 1.11 Cases Cross-Referenced Against Supply Temperature (1)

Supply Temperature	Case Number
Supply Temperature set such that air is at 22.22°C (72°F) at Exhaust	basecase - 98
22.22°C	99-101

Volume II - Section I - Introduction

Page II - 27

Table 1.12 Cases Cross-Referenced Against Supply Temperature (2)

Supply Temperature	Case Number
6.60	62, 66
11.00	29
14.80	63,67
16.80	28
17.50	31-36, 65,83-86,93-98
18.80	basecase -09, 16-27, 37-61, 69-75, 79-82, 90-92
18.90	64, 68
19.20	76-78, 87-89
19.80	30
20.70	10-15
22.22****	99-101

**** In these cases, the supply air temperature was set to 22.22 °C (72 °F). In all other cases, the exhaust air temperature was set to be 22.22 °C (72 °F) by setting the supply air temperature appropriately.

Volume II - Section II - Summary of Results of Whole Room Runs

Page II - 29

2. SUMMARY OF RESULTS OF WHOLE ROOM RUNS

Discussion of these figures and tables can be found in volume I, section 3.1.

Figure 2.01 Mean Room Breathing Zone Temperature

Figure 2.02 Mean Cage Temperatures

Volume II -30

Ventilation Desien Handbook on Animal Research Facilities Using Static Microisolators

Figure 2.03 Mean Room Breathing Zone Relative Humidity (RH)

Figure 2.04 Mean Cage Relative Humidity (RH)

Volume II - Section II - Summary of Results of Whole Room Runs

Page II ■ 31

Figure 2.05 Mean Room Breathing Zone CO2 Concentration

E 2000 :

Q.

Q.

o

o 1500 j

Figure 2.06 Mean Cage CO 2 Concentrations

Volume II -32

Ventilation Design Handbook on Animal Research Facilities Using Static Microisolators

Figure 2.07 Mean Room Breathing Zone NHj Concentration on Day 4

Figure 2.08 Mean Cage NH3 Concentration on Day 4

Volume II - Section II - Summary of Results of Whole Room Runs

Page II - 33

Base case Case 02 Case 03 Case 04 Case 05 Case 06 Case 07 Case Od Case 00 Case 10 Case 11 Case 12 Case 13 Case 14 Case 15 Case 16 Case 17 Case 18 Case 10 Case 20 Case 21 Case 22 Case 23 Case 24 Case 25 Case 26 Case 27 Case 28 Case 20 Case 30 Case 31 Case 32 Case 33 Case 34 Case 36 Case 36 Case 37 Case 38 Case 39 Case 40 Case 41 Case 42 Case 43 Case 44 Case 45 Case 46 Case 47 Case 48 Case 49 Case 50 Case 51 Case 52 Case S3 Case 54 Case 55 Case 56 Case 67 Case 56 Case 59 Case 60 Casa 61 Case 62 Case 63 Case 64 Case 65 Case 66 Case 67 Case 68 Case 69 Case 70 Case 71 Case 72 Case 73 Case 74 Case 75 Case 76 Case 77 Case 78 Case 76 Case 80 Case 81 Case 82 Case 83 Case 84 Case 85 Case 86 Case 67 Case 88 Case 86 Case 90 Case 91 Case 92 Case 93 Case 94 Case 95 Case 96 Case 67 Case 96 Case 99 Case 100 Case 101

1 2

0.02 0.04

0.03 0.07

0.03 0.06

0.03 0.06

003 0.05

0.04 0.07

0.03 0.06

0.03 0.06

0.05 0.06

0.02 0.03

0.03 0.05

0.01 0.02

0.02 0.04

0.03 0.05

0.03 0.05

0.02 0.04

0.04 0.08

0.04 0.08

0.03 006

0.04 0.08

0.03 0.06

0.03 0.06

0.03 0.06

0.04 0.08

0.03 0.06

0.02 0.04

0.03 0.06

0.06 0.11

0.07 0.14

0.03 0.06

0.06 0.11

0.06 0.13

0.07 0.14

0,07 0,14

0.06 0.12

0.07 0.14

0.03 0.06

0.03 0.06

0.04 0.07

001 0.02

0.03 0.05

0.03 0.06

0.03 0.07

0.04 0.07

0.03 0.05

0.02 0.04

0.02 0.04

0.03 0.07

0.03 0.05

0.03 0.06

0.04 0.07

0.03 0.07

0.03 0.06

003 0.06

0.03 0.06

0.04 0.07

0.03 0.06

0.04 0.07

0.01 0.01

0.03 0.06

0.04 0.07

0.16 0.35

0.11 0.22

0.05 0.09

0.07 0.14

0.17 0.32

0.11 0,20

0.05 0.09

0.03 0.05

0.01 0.02

0.03 0.05

0.03 0.05

0.02 0.04

0.02 0.05

0.03 0.05

0.03 0.06

0-03 0.05

0.03 0.06

004 008

0.05 0.09

0.05 0.09

0.(X 0.12

0,09 0.17

0.06 0.15

0.07 0.13

0.06 0.13

0.02 0.05

0.02 0.05

0.03 0.06

0.04 0.07

0.03 0.06

0.02 0.04

0.05 0.09

0.06 0.12

0 04 0.07

0.05 0.10

0.06 0.11

0.04 006

0.01 0.03

0.02 0.04

0 15 0.29

Day by day room breathing zona average NH3 3 4 5 6 7 6 9

0.06 0.10 0.10 0.10 0.08 0.11 0.09 0.10 0.14 0.05 0.07 0.03 0.08 0.06 0.07 0 06 0,12 0.12 0.00 0.12 0.10 0.10 0.10 0.13 0.09 0.05 0.10 0.18 0.22 0.10 0.17 0.10 0.22 0.21 0.19 0.21 0.09 0.10 0.11 0.03 0.08 0.10 0.10 0.11 0.06 0.07 0.06 0.10 0.06 0.09 0.10 0.10 0.09 0.09 009 0.11 0.09 0.11 0.02 0.09 0.11 0.53 0.34 0.15 0.21 0.50 0,31 0.15 0.08 0.04 0.08 0.07 0.06 0.07 0.07 0.09 0.08 0.09 0.13 0.14 0.14 0.18 0.26 0.24 0.21 0.19 0.07 0.07 0.10 0.11 0.09 0.06 0.13 0.18 0.11 0 16 0.17 0.13 0.04 0.06 0.44

0.11 0.15 0.14 0.15 0.12 0.16 0.16 0.16 0.20 0.07 0.12 0.04 0.11 0.14 0.11 0.10 0.20 0.20 0.16 0.18 0.16 0.14 0.14 0.16 0.22 0.16 0.24 0.25 0.32 0.14 0.24 0.28 0.32 0.41 0.34 0.39 0.14 0.16 0.16 0.06 0.12 0.16 0.17 0.21 0.14 0.12 0.11 0.16 0.14 0.14 0 18 0 18 0.18 0.17 0.17 0 18 0.13 0.16 0.04 0.18 0.16 0.77 0.58 0.21 033 0.72 0.57 0.21 0,12 0.06 0.12 0.11 0.09 0.11 0.13 0.14 0.13 0.15 0.18 0.20 0.20 0.27 0.49 0.34 0.33 0.29 0.10 0.11 0.14 0.18 0.14 0.10 0.24 0.31 0.18 0.28 0.29 0.23 0.06 0.09 0.64

0.18

0.23

0.20

023 0.16 0.23 0.27 0 26 0.28 0,10 0 19 0.07 0.18 0.21 0.16 0.15 0.32 0.32 0.25 0.27 0.26 0.20 0.20 0.26 0.41 0.32 0.45 0.36 0.44 0.20 0.35 0.39 0.45 0.69 0.56 0.65 0.21 0.22 0.27 0.09 0.16 0.23 0.26 0.35 0.23 0.19 0.16 0.30 0.23 0.22 0.30 0.28 0.32 0.28 0.30 0.29 0.16 0.22 0.07 0.32 0.23 1.08 0.94 0.29 0.49 1.01 0.93 0.29 0.16 0.08 0.17 0.17 0,14 0.17 0.21 0.23 0.21

024 026 0.29 0.28 0.40 0.82 0-48 0.49 0.43 0.14 0.16 0.20 0.26 0.22 0.15 0.36 0 49 0.29 0 46 0.46 0 39 0.09 0.13 0.90

0.26 0.32 0.26 0.32 0.25 0.31 0.41 0.37 0.36 0.14 0.27 0.10 0.26 0.31 0.23 0 22 046 0.46 0.36 0.39 0.37 0.27 0.28 036 0.63 0.51 0.70 0.50 062 0.28 0.49 0.55 0 63 1.03 0.82 0.96 0.30 0.32 0.38 0.13 0.26 0.33 0.40 0.61 0.33 0.28 0.26 0.45 0.34 0.32 0.44 0.41 0.49 0.42 0.45 0.42 0.25 0.31 0.11 048 0.32 1.50 1.37 0.41 0.70 1.40 1.37 0.41 0.22 0,11 0,24 0.23 0.20 0.24 0.30 0.33 0.30 0.35 0.36 0.40 0.39 0.66 1.22 0,67 0,70 0.61 0.20 0.21 0.27 0.40 0.32 0.22 0.66 0.71 0.41 0.68 0.57 0.57 0.12 0.18 1.25

0.40 0 45 0.38 0.46 0.38 0.43 0.61 0.54 0.53 0.19 0.39 0.14 0.39 0.45 0.32 0.31 0.66 0.87 0.54 0,55 0.54 0.37 0.38 0.49 0.99 0.81 1.10 0.66 0.94 0.38 0.68 0.75 0.86 1.55 1.21 1.44 0.42 0.45 0.65 0.20 0.36 0.46 0.59 0.76 0.49 0.42 0.38 0.67 0.50 0.46 0,65 0.60 0.74 0.62 0.63 0.62 034 0.43 0.16 0.74 0.45 2.05 2.02 0^8 0.99 1.91

2.04 0.56 0.31 0.16 0.34 0.33 0.28 0.34 0.45 0.48 0.44 0.51 0.49 0.54 0,54 0.79 1.84 0.91 1.00 0,85 0.27 0.29 0.37 0.57 0.48 0.31 0.82

1.04 0.60 1.01 0.98 0.35 0.16 0.24 1.71

0.49

0.59

0.52

0.60

0.47

0.53

0.76

0.69

0.71

0.26

0.51

0.18

0.49

0.58

0.42

0.40

0.85

0.85

0.68

0.72

0.69

0.50

0.51

0.66

1.19

0.9S

1.31

0.92

1.14

0.52

0.90

1.01

1.16

1.92

1.52

1.79

0.55

0.59

0.71

0.25

0.47

0.62

0.75

0.95

0.62

0.53

0.48

0.S4

0.63

0.59

0.82

0.76

0.91

0.78

0.84

0.79

0.46

0.58

0.20

0.90

0.59

2.77

2.55

0.75

1.30

2.58

2.55

0.75

0.41

0.21

0.45

0.43

0.37

0.45

0,57

0.61

0.56

0.64

0.66

0.73

0.72

1.04

2.28

1.23

1.30 1.13 0.36 0.39 0.50 0.74 0.59 0.41 1.04 1.33 0.77 1.27 1.25 1.06 0.22 0.32

2.30

0.59

0.76

0.69

0.77

0.60

0.77

0.91

0.86

0.94

0.33

0.63

0,23

0.59

0.72

0.55

0.50

1.06

1.06

0.84

0.92

0.85

0.66

0.60

0.88

1.36

1.05

1.40

1.22

1,51

0.68

1.19

1.34 1.54

2.29 1.85

2.15 0.71 0.75 0.90 0.31 0.81 0.79 0.93

1.15 0,76 0.64 0.58 1.01 0.77 0.74 1.00 0.94 1.07 0.94 0.99 0.98 0.62 0.76 0.24

1.05 0.78 3.66 3.13 1.00 1.65 3.42 3.10 1.00 0.55 0.28 0.58 0.56 0.47 0.57 0.69 0.76 0.70 0.79 0.87 0.97 0.96

1.35 2.73

1.64

1.65 1.46 0.46 0.51 0.67 0.93 0.75 0.52 1.27 1.64 0.96 1.54 1.54

1.29 0.29 0.43

3.06

10

0.63

0.95

0.90

0.94

0.74

1.01

0.99

1.01

1.23

0.42

0.75

0.27

0.64

0.83

0.70

0.58

1.23

1.25

0.96

1.13 0.99 0.66 0.89 1.15

1.30 0.92 1.39 1.59 1.97 0.89 1.52 1.75 2.01 2.44 2.06 2.34 0.07 0.93 1.08 0.35 0.75 0.96 1.06 1.27 0.86 0.72 0.66 1.11 0.86 0.68 1.12

1.07 1.10

1.03

1.04 1.12 0.61 0.97 0.26

1.08 0.09

4.81 3.54

1.31 2.01 4.47

3.42

1.31 0.72 0.35 0.74 0.70 0.56 0.71 0.79 0.68 0.81 0.90

1.14 1.27 1.25 1.70 2.94

2.14 2.00

1.82 0.63 0.67 0.87 1.11 0.69 0.63

1.43 1.89 1.11 1.70 1,79 1.42 0.38 0.56 4.00

1 2

0-07 0.14

0.09 0.18

0.09 0.17

0.09 0.17

0.06 0.16

0.08 0.16

0.20 0.38

0.12 0.23

0.14 0.28

0.08 0.16

0.12 0.23

0.12 0.23

0.15 0.28

0.12 0.22

0.16 0.31

0.13 0.25

0.10 0.18

0.10 0.18

0.12 0.22

0.09 0.16

0.10 0.19

O.n 0.22

0.09 0.16

0.22 0.43

0.13 024

0.08 0.16

0.13 0.24

0.16 0.31

0.14 0.27

0.13 0.25

0.16 0.32

0.18 0.34

022 0.44

0.17 0.32

0.15 0.29

0.16 0.31

0 09 0.18

0.10 0.20

0.13 0.25

0.05 0.09

0.07 0.14

0,10 0.19

009 0,17

0.13 0.24

0.13 0.25

0.11 0.20

0.09 0.18

0.16 0.31

0.13 0.25

0.10 0.20

0.12 0.23

0.19 0.37

0.09 0.17

0.15 0.29

0.13 0.24

0.08 0.16

0.15 0.26

0.13 0.26

0.05 0.09

0.09 0.16

0.16 0.31

0.47 0.91

0.34 0.66

0.17 0.33

0,17 0.32

0.43 0.83

0.33 0.63

0.14 0,26

0.14 0.26

0 06 0.12

0.16 0.31

0.13 0.26

0-08 0.15

0.13 0.25

0.13 0.25

0.12 0.24

0.10 0.19

0.11 0.21

0.10 0.20

0.11 0.21

0.14 0.28

0.17 0.34

0.21 0-41

0.20 0.38

0.23 0.45

0.20 0.38

0.07 0.14

0.04 0.09

0.11 0.22

0.15 0.28

0.10 0.19

0 06 0 IS

0.19 0.37

0.17 0.33

0.15 0.28

0.15 0.28

0.15 0-30

0.18 034

0,06 0.12

0.10 0.19

0.41 0.60

Day by day room breathing zone maximum NH3 3 4 5 6 7 8 9

0.21 0.26 0.27 0.26 0.24 0.25 0.58 0.35 0.43 0.25 0.35 0.36 0.44 0.34 0.48 0.39 0.28 0.28 0,35 0.27 0.29 0.34 0.28 0.67 0.36 0.23 0.37 0.46 0.42 0.39 0.49 0.53 0.67 0.50 0.45 0 47 0.28 0.31 0.39 0.13 0.22 0.30 0.26 0.37 0.36 0.31 0.27 0,47 0.38 0.31 0.38 0.S7 0.26 0.44 0.36 0.26 0.44 0.40 0,14 0.27 0.48 1.40 1.01 0.51 0.50 1.28 0.97 0.41 0.41 0.19 0.48 0 40 0.23 0.39 0.39 0.37 0.30 0.33 0.30 0.32 0.43 0.52 0.63 0.59 0.69 0.58 0.21 0.13 0.34 0.44 0.29 0.24 0.56 0.51 0.44 0.44 0.46 0.52 0.19 0.30 1.24

0.40 0,43 0.38 0.42 0.37 0.35 1.07 0.53 062 0 37 0.57 0.59 0,81 0.58 0.71 0.63 0.47 0.47 0.59 0.43 0.49 0.49 0.41 0.96 0.36 0.69 0.93 0.69 0.60 0,57 0.72 0.76 0,97 0.95 0.81 0.38 0.43 0.47 0.62 0.23 0.34 0.47 0.44 0.67 0,67 0.55 048 0.86 0.68 0.51 0,63 0.98 0.53 0.81 0,74 0.42 0.63 0.58 0.27 0.56 0.72 2.02 1.76 0.73 0.79 1.84 1.75 0.59 0.59 0.29 0.71 0.60 0.36 0.59 0.67 062 0,50 0.57 0.44 0.47 0.63 0.78 1.18 0.85 1.10 0.89 0.30 0.19 0.49 0.70 0.46 0.37 0.98 0.87 0.74 0,79 0.77 0.94 0.28 0.43 1.78

0.63

0.63

0,54

0.62

0.56

0.50

1.79

0.91

0.38

0.53

0.38

0.92

1.36 0 91

1.03 1.02 0.73 0.73 0.95 0.64 0,77

0 69 0.57 1.35

1 60 1.39 1.77 0,97 0.35 0.80

1.04 1.07

1.37 1.61

1.32 1.47 0.64 0.70 0,94 0.37 0.50 0.70 0.70 1.11

1.07 0.89 0.78 1.42 1.10 0.79 1.03 1.57 0.93

1.33 1.26 0,66 088 0.84 0.45 0.99 1.03 2.64 2.83 1.02 1.18

2.59 2.86 0.83 0.32 0.42 1.02 0.87 0.55 0.88

1.08 0.97 0.79 0.91 0.62 065 0.68 1.14 1.99 1.20 1.66 1.30 0.42 0.27 0.69 1.08 0.71 0.66

1.59

1.38 1.16 1.30 1.22 1.54 0.39 0,61 2.51

1.01

0.88

0.75

0.89

0.79

0.69

2.65

1.31 1.21 0.75

1.27

1.32 2.01

1.32 1.45 1.48 1.06 1.05 1.38 0.91 1.11 0.97 0.79 1.87 245 2.16 2-72

1.35

1.17 1.10 1.45 1 48

1.90

2.40 1.95

2.18 0.90 1.00 1.34 0.54 0.70 1.00 1.01

1.65

1.56 1.31 1.14 2.10 1.61

1.14 1.51 2.29

1.40 1.97 1.89 0.96 1.22 1.17 0.67 1.48 1.45

3.94

4.14

1.42 1.68 3.59

4.25

1.15 1-14 0.58

1.43 1.23 0.78

1.25

1.57 1.40 1.14

1.33 0.86 0.91 1.22 1.61

2.95

1.67

2.36 1.83 0.69 0.38 0.95 1.55 1.03 0.60

2.33 2.01

1.68

1.91 1.77

2.28 0.54 0.84 348

1.51

1.24

1.02

1.27 1.12 0.94 3.97

1.90 1.66 1.05 1.34

1.92

3.02

1.93

2.02 2.16 1.54 1.53

2.03

1.29 1.62 1.33 1.08

2.56 3-83

3.50

4.29

1.84 1.60

1.51 2.00 2.02 2.60 3.63 2.69

3.26

1.28

1.41

1.91 0.79 0.99

1.43 1.49 2.46

2.30

1.94 1.66

3.14

2.36 1.6S

2.23

3.37

2.14

2.94 2.86

1.41 1,68' 1.62 1.01

2.27 2.01

5.39 6.10

1.94

2.39

4.91

6.32

1.57

1.56 0.82 1.99 1.72 1.12 1.77

2.32

2.04 1.67

1.95 1.17

1.24 1.67

2.25

4.44

2.28 3.37

2.57 0.81 0.51

1.30 2.24 1.48

1.14

3.43 2.94

2.44

2.85 2.59

3.39 0.74 1.16 4.76

1.88

1.64

1.38

1.65

1.47 1.27 4.94

2.43

2.24

1.39 2.36 2.46

3.75

2.45

2.69

2.76 1.97

1.96 2.57 1.68 2.06

1.78

1.46

3.46 4-59

4.10

5.11

2.48 2.18

2.04 2.68 2-73 350

4.49

3.63

4.06

1.66 1,85 2.48 1.00 1.30

1.05

1.69

3.07

2.91

2.44

2.12

3.92

3.00 2.12 2.81

4.26 2.62

3.67 3.53

1.79

2.26 2,16

1.25

2.77

2.68

7.27 7.71 2.62

3.12

6.63

7.92 2-12 2.11

1.08 2.65

2.27

1.45

2.32

2.93 2,61

2.13

2.47

1.56

1.68

2.25

2.97 5.51 3.07

4.38

3.39 1.09 0.69 1.75

2.89

1.90

1.48

4.33 3.73 3.12

3.56 3.29 4.24

1.00

1.56 6.42

2 25

2.12

1.83 2,10 1.88 1.69

5.94 3.04 2.98 1.81

2.96 3.08

4.51 3.04

3.50

3.43

2.46

2.44 3.16 2.15

2.56 2.36

1.94

4.59

5.23

4.52 5.77

3.30 2.66

2.71

3.51

3.63

4.65

5.35

4.41

4.86

2.15 2 38

3.15

1.23 1.68

2.36

2.33

3.71

3.57

2.98

2.59 4,73

3.66

2.65

3.42

5.24

3.08

4.43 4.18 2.21 3.00

2.83

1.49

3.25

3.50

9.65

9.46 3.48

3.97 8.81

9.60 2.81 2.80 1.41

3.46

2.95

1.84

2.96

3.60 3.24

2.64 3.04 2.10 2.23

2.99

3.86

6.60

4.08

6.57

4.39

1.44 0.92

2.33 3.63

2.39 1.88

5.30 4.61

3.87

4.32 4.08 5.14

1.33 2.07

8.53

10

2.43

2.65

2.39

2.56

2.30 2.21

6.46

3.56

3.89

2.29

3.49

3.63

4.90 3.52

4.43

4.00 2.87 2.86

3.62

2.62

2.98

3.06

2.64

6.00

5.01 3.96

5.40

4.31

3.76

3.54

4.51

4.74 6.08

5.70

4.91

5.30

2.66 2.93 3.80

1.40 2.08

2.87 2.66

4.07

4.05 3.34

2.92 5,19

4.11

3.11 3.83 5.95 3.18

4.87

4.40

2.54 3.62 3 63 1.60

3.33

4.47 12.61 10.71

4.55

4.85

11.51

10.01 368 3.66 1.78 440

3.70 2.22

3.68 4.07

3.77

3.06 3.46

2.74

2.91

3.91

4.86

7.11

5.34

6.75

5.49

1.69 1.21 3.04 4.33 2.85 2.29

5.98

5.31

4.49

4.77

4.72 5.68

1.73

2.71 11.15

Table 2.01 Room Breathing Zone NH3 Mean and Maximum Concentrations for 10 Days

Volume II -34

Ventilation Design Handbook on Animal Research Facilities Using Static Microisolators

Basocase Casa 02 Case 03 Case 04 Casa 05 Casa 06 Case 07 Casa 08 Case 09 Case 10 Casa 11 Casa 12 Casa 13 Casa 14 Casa 15 Case 16 Casa 17 Casa 18 Casa 19 Casa 20 Casa 21 Casa 22 Casa 23 Casa 24 Cass 25 Casa 26 Case 27 Casa 25 Casa 29 Case 30 Casa 31 Casa 32 Casa 33 Casa 34 Casa 35 Casa 36 Casa 37 Case 38 Casa 39 Casa 40 Casa 41 Casa 42 Casa 43 Case 44 Casa 45 Casa 46 Casa 47 Casa 48 Case 49 Casa 50 Casa 51 CasaS2 Casa 53 Casa 54 Casa 55 Case 56 Case 57 Cose 56 Casa 59 Case 60 Casa 61 Casa 62 Casa 63 Case 64 Case 65 Casa 66 Casa 67 Casa 66 Casa 69 Case 70 Case 71 Casa 72 Casa 73 Casa 74 Casa 75 Casa 76 Casa 77 Case 76 Case 79 Case 80 Case 81 Casa 62 Case 83 Case 84 Case 85 Case 86 Caoa 67 Case 88 Case 89 Casa 90 Casa 91 Casa 92 Case 93 Casa 94 Casa 95 Casa 96 Casa 97 Casa 98 Casa 99 Casa 100 Casa 101

1.15 2.18

0.89 1.72

0.82 1,59

0.90 1.73

0.91 1.76

0.86 1.67

1.24 2.35

0.96 1.89

0.90 1.75

1.11 2.14

1.11 2.14

1.16 2,23

1.33 2.52

1.17 2.24

1.11 2.14

1.17 2.24

0.96 1.84

0.94 1.81

0.91 1.75

0.87 1.68

0.95 1.63

0.87 1.69

0.86 1.67

0.98 1.91

1.64 3.04

2.25 4.02

1.75 3.21

0.91 1.77

0.92 1.79

0.87 1.69

1.01 1.96

0.91 1.77

1.03 2.00

1.01 1.92

0.93 1.70

1.00 1.90

0.90 1.74

0.93 1.80

0.97 1,87

1.04 2.00

0.88 1.71

0.91 1.75

0.98 1.88

1.13 2.14

1.02 1.95

1.03 1.97

1.04 1.98

1.12 2.12

1.07 2.05

0.97 1,86

1.08 2-07

0.68 1.69

1.19 2.24

0.99 1.89

1.09 2.06

1.00 1.91

0.96 1.66

1.04 2.01

1.15 2.19

1.27 2.38

1.06 2.07

1.21 2.35

1.10 2.11

0.94 1.83

1 .05 2.03

1.21 2.35

1.14 2.17

0.96 1.66

0.91 1.77

1.02 1.98

1.03 1.99

0.93 1.81

1.03 1.99

0.99 1.91

0.97 1 86

0.98 1.86

0.99 1.90

1.01 1.94

0-86 1.67

0,89 1.73

0.91 1.76

0.87 1.69

0.94 1.78

0.96 1.88

1.01 1.95

1 .00 1 .94

0.72 1.41

0.81 ^St

0.65 1.66

0 68 1 69

0.90 1.73

0 86 1.65

0 96 1.84

0.93 1.78

0.96 1.84

1.04 1.98

0.91 1.75

1.04 1.98

1.03 2.01

0-90 1.75

1.25 243

3

3.37

2.66

2.45

2.67

2.72

2.56

2.69

3.31

3.30 3.46

3.89 3.46

3.31 3.45

2.83 2.79

2.69 2.58 2.82 2.60 2.SB

2.94

4.69 6.20

4.95 2.73 2.75 2.60

3.02 2.73 3.08

2.96 2.75

2.93 2.68 2.77 2.88 3.08 2.64

2.70

2.90 3.31 3.01

3.03

3.06 3.28 3.16 2.87 3.19 2.60 346

2.92

3.16

2.94

2.89 3.10 3.37

3.67 3.19 3.63 3.2S 2.82 3.12 3.62 3.34

2.67

2.73 3.05

3.07 2.79

3.07 294 2.87

2.90

2.93 2.99 2.57 2.66 2-72 2.60

2.74 2.89 3.01 2.99

2.17 2.42 2.55 2.61 2.67 Z.5S

2.84 2-74 2.84 3.05

2.69 3.05 3.10

2.70

3.74

Day by day cage average NH3

4 S 6 7 8 9 10

6.33

4.03

3.53 4.18

4.23

3.72

6.71

4.83 3.88 4.94

5.42 5.66

7.21 S.B2 4.94

5.76

4.71 4.62 4.61

4.04

4.73

3.77

3.71

4.24 11.05

16.42 12.31 3.93

3.97

3.75 4.45

3.93

4.44 5.66 4.90

5.44 4.13 4.26 4.58

5.33

4 06

4.25 4.96 6.03

5.22 5.35 536

6.98

5.56 4.73 5.64

4.49

7.02 5.32

6.22

5.01 4.17 4.58 6.38

7.56 4.73 5i3

5 64 4.07 4.90

5.21

6.03

4.13

3.94 4.58

4.56

4.22 4.87

4.53

4.96 4.85

4.94

5.16 3.70

3.84 3.92 3.92 5.15

4.17

4.76

4.54

3.13

3.49 3.68 4.22 433

4.02

4.97 4 66

4.77 531 4.54

5.50 4 46 3,89 5.40

10.66

5.92

4.96

6.29 6.32 5.22 1131

7.53 5.46

7.17

8.40

6.78 12.05

9.17

7.17 9.02 7.38 7.19

7.36 6.06 7.44 534 5.22

5.96 20 64

37.17 23 47

5.53

5.58 537

6.37

5.53 6.24 9.63 8.01 9.09

6.14 6.36

6.94 655 6.01

6.41 7-90 997 6 40 8.66 867 9.91

9.02 736

9.17 730

12.29 8.60 10.69

7.95 5,07 6.56

10.78 1331 6 81 736

9.06

5.72 736 733 9.62 5.81

5.54 6 66 6.56

6.15 739

6.72 0.01 7.61

7.78 835 531

5.40 531

5.72 B.S7 5. 86 7.19 6 66

4.40 4.90

5.17 6.48 6.65

6.06

8.02 730 7.50 9.06

7.16 9.05 638 S.47

7.59

15.82

8.35 6.88 8.96 8.99 7.24 16.60 10.88 7.57 10.06 12.10 12.68 17.85

1330 10.06

13.05

10.67 10.39 10.72 8.62 10 79 7.42 734 8.26 31.63 5831 36.19

7.67

7.74

731 8.89

7.66

6.66 14.34 11.77 13-47 0.70

9.02

9.92 12.46 8.51

9.14 1130

14.71 1237

12.72 12.68

14.62

1331

10.62 13.45 1031 1848 12.98 15.97

11.56

8.14 9.16

16.02

20.05 934 1030 1336

7.93 10.48

10.17 14,61 8.06

7.68

9.36

932 8.67

10.57

933

11- 70

11.03 11.29

12.03

732 7.49 7.64 8.06

12- 07 8.13 1036 939 6.10 6.80

7.17 932 937 8 65

11.74

10.73 10 87

13- » 1039 1333 8 70 739 10.53

23.70

11.72 9.41

12.75 12.‘^

9.92

24.07

16.79 10.36 14.W

1731

26.75

19.40

14.03 16.99 15.52

15.07

15.74 12.26

16.72 10.20

9.91 1130

49.49 93.38

57.03

10.50 10.59 10.01 1230 10.49

11.85 21.65

17.46 20.19 12.31

12.76

14.18 1636 12.02

13.03

16.87 21 96

18.08 18.82

10.73

21.83 1934

15.40 19.61 15-47

28.24 1937 2432 16.93

11.13 12.68

24.13

30.71 1336 13.96 19.55

10.85

14.92

13.91

21.75

11.03 1031 13.08 12.82

12.14 16.12 13-46

17.24 18.07

16.47 17.67 9.68

10.24 10.45 11.28 1034 11.12 14.64

13.19 835 930 9.82 13 41

13.79 12.33

1732

15.71

15.84

19.87 15.18

19.85

11.91 1038

2931

15.45

12.70 16.61 16.66 13.37

30.95 20.22

13.97 18-61

22.48 23.52 3339

24.73 18.61 24.26

19.64

19.30

19.95

15.98

20.05

13.71

13.37

15.25

59.31 109.47

67.01

14.16 14.28 13.50 1644 14.15

16.98

26.77 21.62

25.12

16.12

16.71 1639 2332

15.77 16.94 21.40

27.43

22.64 23.69 23.62

27.26

24.61

10.74

25.05 19.57

34.54 24 30 29 63 2130

16.02

16.03

29.90

37.49

17.64 1633 24 60

14.64

19.44

18.77 27.23 14.89

14.17

17.32

17.06

16.04

10.61 17.te 21.79 2031 20.08

22.38 1333

13 82 14.10

14.91

24.01 1500

19.02 1739 1136

12.55 1335 1739 17.76 1604

21.85 19.97 2031 24 88

19.33

24.85 1607

14 01 16.43

35.35 19.99 16.87

21.17 21.31 17.76

37.22

25.23

18.55 24.25 28.16

29.43

40.02

30.67

24.25 30.19 24.70 24.10

24.59 20.40

24.90 18.13

17.76

20.25

67.69 120.68

76.69 18.81 18.97 17.93

21.59

10.79

21.23

31.90

26.67 30.19

20.70 21.45 23.33

26.53 20.28

21.56 26.38 33.16

28.03

26.92

28.89

32.92 30.06

24.67

30.66

24.04

40.56

29.24

36.36

26.67

19.95 22.23

35.76

43.90

23.00

25.01

30.29

19.44

24.79

24.92

33.01

19.77 18.82

22.53

22.30 20.81 24 66 22 68 26.73

25.49 26.03 27.64

17.71

18.35

18.72

19.35

20.75

19.93

24.18

22.50

14.96

16.67

17.59

21.77 2233 2039

26.75

24.68 26.09 30.15

23.96 30.12 2134 10.61 26.81

38.09 25,01

22.05

25.80 26.14 23.22

40.46

29.56 24.25 30.72

33.21 34.67

43.47 35.51

30.72 35.20 28.62 28.27 26.11 24.94 28.90

23.55

23.22

26.47

64.80 105.66

71.78 24.59

24.80

23.44

27.73

24.57

27.75

34.00

29.69

32.78 25.65

26.46

28.19

32.42

25.10

26.19

30.19 36.40

31.77

32.44

32.55

36.12

33.76

28.99

34.22

27.33

41.81

32.12 37.24 30.53 26.08 26.50

30.37 44.97

29.42

32.69

34.30

25.42

30.23

32.56 36.49 25.84 24.61

28.44

28.39 26.16

30.01 28.03

30.30 26.63

30.16

31.38 2315 23 99

24.47

24.34

30.99

26.05

29.32

26.16

19.55

21.78 23.00 25.93

26.55 24.75

30.10

28.40

29.12

33.32

27.70 33.29 27.89

24.32

33.74

Pay by day cage maximum NH3

1 2 3 4 5 6 7 6

1.67

1.29

1.34 1.20 1.22

1.38 1.80

1.39

1.52 1.67

1.53

1.59

1.60 1.38 1.67 1.65

1.30 1.26

1.24 1.23

1.35

1.37 1.33

1.57 2.15 2.73

2.51 1.41

1.33

1.34

1.46 1.45

1.63

1.44

1.43

1.38 1.17

1.36

1.41

1.47

1.25 1.33 1.28

1.45

1.42

1.45

1.48

1.44 1.42 1.36 1.44

1.39

1.52

1.42

1.40 1.36

1.43

1.50

1.46

1.58

1.47 1.76 1.60 1-36 1.65 1.60 1.81

1.51 134

1.43 1.40 1.39

1.30

1.39

138

1.40 1.39 132 1>40 1>41

1.46 1.61

1.54

1.49 1.70 137

1.31

1.35 132

139 136 1>47 1>44

1.47 1.47 132

1.44

1.64 1.72 1.83

3.17

2.61

2.61

2.32

236

2.68

3.43

2.67 2.97

334 2.94 3.06 3.04

2.65 3.24

3.16

2.46

2.47 2.36

236

2.59

2.66 238 3.06

3.96

4.90

4.61

2.74

2.69

2.61 2.87 2.82

3.17 2.72 2.72

2.63 2.27

2.70 2.72 2.61 2.41

237 2.46

2.77

2.71

2.78

2.64

2.75

2.72 2.63

2.74 2.66 2.86

2.71 2.66

2.60 2.77

2.90 2.77

2.96 2.66

3.43 344 ^65 3.19 331

3.45 2.93 2.61 2.77

2.72 2.69

2.73 2.66

2.65

2.46

2.68

2.66

238 2.72

2.75 2.83

3.06 3.00

2.07 3.29 2.67 235 2.63 2.54 266

2.43 2.81 2.77 2.61 2.80 2.92

2.74 3.00

335

336

4.89 387 4.03 338 3.64 4 13 5.28 4.12 4.57 5.00 4.53 4.72

4.66 4.09

5.00 4.68 384 3.80

3.67 3.67 3.99 4.09

3.98 4 72

6.15 7.55

7.11 4.22

3.99 4.02 4.43

4.35 4.89 430 4.20 4.05 330

4.16 4 19 434

3.71 3.96

3.79 4 26

4.18 4 28

436 4.24

4.19 4.05

433 4.09 4.41

4.18 4.09 401

4.26 4.46

4.27 4.57 4.41 539

5.31

4.00

4.91 5>41 531 4.52 4 03 438

4.19

4.15 4.22

4.11

4.09

3.80 4 14

4.10 3.67 4.19 4.24

4.36

4.72 4.62 4.43 5.07

4.12 3.93 4.05

3.92

4.12 3.7S 4.34

437

434

4.31

4.50 433 4.62

5.16

5.50

0.19

5.07

5.81 5.62

5.67

5.95 9 79

6.81

6.59 7.47 7.43 7.7S

8.69 6.86

7.47 8.13

6.40 6.28

6.29

5.74

6.69 5.94

5 74

6 80

14.47 22 42 17.70

6.08

5.75 5.79 6.52 637

7.05 B.03

7.49 731

5.41

6.42

6.65

7.50

5.72

6.23

6 49

7 77 736

736

7.67

7.73

737

6.67

7.40

7.06

6.96

7.61 8.00 6.82 6.17

6.60

6.09

9.40 6.54

7.62

9.23

6.69

7.70

7.60 936 6.52

5.61 642 632

637

6.70

6.33

7.10 636 6.96

7.05

5.30

6.05 6.12

638 8.87 6 67 7.00

7.70 6.93

6.66 5.85

6.34 6 69

5.92

7.58 734

738 7.78

7.59 7.64 6.66 743

7.92

15.47

8.61

8.16

8.45

6.46 837 16.35 10.62 9.27

10.83

11.51 12.02

14.52

10.84

10.83

12.74 10.01 9,78 10.04 8.00

10.52

6.41

8.07

9.56 27.02

45.23

33.74

8.56

8.08

8.15

9.33 8.62 9.91

13.66

12.24

12.56 8.03 9.54 10 08 12-02

8.47 9.40 10.34

12.66 11.68 12.26 1240 12.81 11.95 1037 12.16 1135 16.70 12.60

13.76

10.84 8.67

9.45 13.66

16.57

9.42 10.72

14.84 636

1137

10.97

15.76

9.17

8.17

6.33

8.97

9.16

10.16 9.39 11-42

9.98

10.97

1138

7.45 8.60 8.60 9 59 14.92 9.37 1037 1138 0.34 7.96 832 9.73 10.29 8.93 1234 11>49 11,44 12.80

11.98 1236 9.36 10.46 11.14

22.96

12.14

11.32 12.03

12.03 11.60

24.20

15 34 12.86

15.20 16.59

17.33 21 .50 15.72

15.20 18.43 14.46

14.12 14.63

12.25 15.24 11.69 11.16

13.26 41.42 70.83

52.03 11.86

11.21

11.30

13.03 12.23

13.74 2034

17.99

16 60 1138

13.54 14.40

17.55

11.99 1340 15-06

18.96 17 06

17.07

18.15 18.91 1731

14.97 17.82

16.56 23 61 1839 20.55

16.76 12.02

13.21

20.30 24.95

1330 14.66 21 69

11.48

16.49

15.21 23.20 12.71 1133

13.12 1238

12.90 14.53

1331 1669 14.46

15.91

16.45 1033 11.79 11.93 1331

22.16 13.00 15.09

15.92 1137

11.04 11>40

13.98

14.76

12.74 17.91 16 70 1639 ie.BS 1739

16.50

12.08 1430

15.45

34.63

17.05

16.50

17.13

17.07 15.86

36.26

22.26

17.60 21.20

24.01 2S.Q9

32.23 22.94 21.20 26.82

21.07 20.48

21.46 17.41 22 22

16.06

15.32

18.14

64.81

113.63

61.98

16.24

15.34

15.47

18.02 16.74

18.61 30.71 26.70 27,88 16.10

19.15

20.59

25.62 16.93 19.11

22.09

28.33

26.13

26.60 26.80 26.23

25.69

21.70 26.39

24.37

36.08

27.76

31.16

23.07 1646 18.29 30.67

38.22

18.33 2034

31.98

15.71 23 46 2032

34.63 1740

15.50 1833 17.46 18.06 20.78

18.81 24.58

21.07

23.22

24.17

14.13

15.14

16.33 18.91 33.31

17.76 21.54

22.35

15.84

15.10 15.60

20.13 2132 16.16

26.44

24.44

24.18 28.06

25.38

27.64 17.76

19.84

21.14

42.64

22.48

20.90 22.31

22.30 21.42

45.13

28.50 23.74 28.12

30.62

32.20 40.10

29.23 28.12 34.26 26.93

26.24

27.21

22.70 28.34

21.59 20.67

24.47

77.66 13331

97.63

21 90

20.70 20.B6

24.08

22.59

25.37

37.98 33.61

34.69

21.09

25.06 26.72

32.66 2231

24.86 28.02

35.38

31.76

33.48 33.79

35.25

32.60 27.81

33.19 30.83

44.13

34.66

38.38

29.31

22.20 24 42

37.69

46.66 24.40 27-44 40.37 21.20

30.67 28.08 43.24

23.47

20.91 2438

23.26

23.87

26.95 24 66

31.06 26.89 29.59

30.61

19.07

21.77

22 03 25.00

41.33 24 00

27.99

29.47

21.36 2037 21 04

26.96

27.47

23 63

33.36 31.06

30.64 35.12

32.33

34.48

23.96

26.77

28.51

Table 2.02 Cage NH3 Mean and Maximum Concentrations for 10 Days

9

51.32

29.08

27.76 28.44

28.53

28.44 54.28

35.57

31.53 36.65

38.60

40.30 48.21

36.26

36.65

42.65

33.54

32.77

33.53 28.98

35.20

28.57

27.45 32.50 8B.S1 146.85

110.26

29.09

27.49 27.71 31.63 30.00

33.69

45.24

40.70

41.70 27.08 32.19 33.89

40.12

28.56 31.63

34.54

42.75

38.96

40.87 41.34

42.58

39.82

34.76

40.49 37.80

51.62

41.68

45.49

36.21 29.48

32.05

45.30

64.63 31 .92

36.44 49.52 26.15

38.97

37.30

52.41

31.17

27.77

31.58

30.40 30.96

34.17

31.69 38,11

33.42

36.70

37.68 25.32 26 91

29.25

32.45

49.60

31.87

35.68

38.13 28.37

27.06 27.95

32.66

34.54

30.06

40.83

36.40

38.30

42.57

40.07 41.79 31.82

35.55

37.87

10

55.30 36.39 36.29 34.67

34.99 37.18

59.00 41.66

41.21 46.42

45.52

47.47 52.37

41.99

46.42

49.71

39.13

36.44

38.34

35.42

40.85 37.11

35.88

42.49

84.85

126.57 103.19 38.02

35.93

36.22

40.61 39.21

44.04

48.23

45.36 45.28

33.42 39,73

40.94

45.60

35.36

36.42

30.52

46.95

44.16

46.64

46.57

46.71

44.72

40.85

45.34

43.05 63.41

46.00

47.92

41.01 38.54 41.10

48.62

55.05

40.69

47.64

56.09 36.80

47.53

46.76

57.95

40.76

36.30

39.88

30.70

38.92 41.25

39.17 43.20

36.85

42.61

42.02

33.10 37.79

38.24

40.61

53.35 41.66

43.14

47.71 37.09

36.37

36.53 38.92

41.07

36.48

46.07

44.18

44.45

47.05 46.34

46.19 41.60 46.47

49.50

Volume II - Section II - Summary of Results of Whole Room Runs

Page II - 35

Cas> Nama 1

Basacasa 0.02

Caaa02 0.03

Casa 03 0.03

Casa 04 0.03

Case 05 0.03

Casa 06 0.04

Casa 07 0.03

Casa 08 0.03

Casa 09 0.05

Casa 18 0.02

Casa 11 0.02

Casa 12 0.01

Casa 13 0.02

Casa 14 0.03

Casa 15 0.02

Casa 16 0.02

Casa 17 0.04

Casa 18 0.04

Casa 19 0.03

Casa 20 0.04

Casa 21 0.03

Casa 22 0.03

Casa 23 0.03

Casa 24 0.04

Casa 25 0.03

Casa 26 0.02

Casa 27 0.03

Casa 26 0.06

Casa 29 0.07

Casa 30 0.03

Casa 31 0 06

Case 32 0.06

Casa 33 0.07

Casa 34 0.07

Case 35 0.06

Casa 36 0.07

Casa 37 0.03

Casa 38 0.03

Cass 39 0.04

Casa 40 0.01

Casa 41 0.03

Casa 42 0.03

Casa 43 0.03

Casa 44 0.04

Casa 45 0.03

Casa 46 0.02

Case 47 0 02

Casa 48 0.03

Casa 49 0.03

Casa 50 0.03

Casa 51 0.03

Casa 52 0.03

Casa S3 0.03

Casa 54 0.03

Casa 55 0.03

Casa 56 0 04

Casa 57 0.03

Casa 58 0.04

Casa 59 O.Oi

Casa 60 0.03

Casa 61 0.04

Casa 62 0.16

Casa 63 0.11

Casa 64 0.05

Casa 65 0.07

Casa 66 0.17

Casa 67 0.10

Casa 68 0.05

Casa 69 0.03

Casa 70 0 01

Casa 71 0.03

Casa 72 0.02

Casa 73 0.02

Casa 74 0.02

Casa 75 0.02

Casa 76 0.03

Case 77 0.03

Casa 76 0.03

Casa 79 0.04

Casa 80 0.05

Casa 81 0.05

Casa 82 0.06

Casa 63 0.09

Casa 64 O.OS

Casa 65 0.07

Casa 86 0.06

Casa 87 0.02

Casa 88 0.02

Casa 89 0.03

Casa 90 0.04

Casa 91 0.03

Casa 92 0.02

Casa 93 0.04

Casa 94 0.06

Case 95 0.04

Case 96 O.OS

Case 97 0.06

Casa 98 0.04

Case 99 0.01

CasalOO 0.02

Casa 101 0.15

2

0 04 0.07 0.06 0 06 0.05 0.07 0.06 006 0.09 0.03 O.OS 0.02 0.04 O.OS

005 0.04 O.OS 0.08 0.06 o.oe 0.06 0.06 0.06 0.06

006 0 04 0.06 0.11 0.14 0.06 0.11 0.13 0.14 0.14 0.12 0.14 0.06 0.06 0.07 0.02 0.05 0.06 0.07 0.07 0.05 0.04 0.04 0.07 0.05 0.06 0.07 0.07 0.06 0.06 0.06 0.07 0.06 0.07 0.01 0.06 0.07 0.35 0.22 0.09 0.14 0.32 0.20 0.09 O.OS 0.02 0.05 O.OS 0.04 0.05 0.05 0.06 0.^ 0.06 0.08 0.09 0.09 0.12 0-17 0.15 0.13 0.13 0.05 0.05 0.06 0.07 0.06 0.04 0.09 0.12 0.07 0.10 0.11 0.08 0.03 0.04 0.29

Day by day room breathing 2ona average NH3 3 4 6 6 7 s'"

0.06 008

0.10 0.15

0.10 0.14

0.10 0.14

0.06 0.11

0.11 0.16

0.09 0.13

0.10 0.14

0.14 0.20

0.05 0.07

0.07 0.11

0.03 0.04

0.06 0.06

0.06 0.12

0.07 0,11

0.06 o.oe

0.12 0.17

0.12 0.18

0.09 0.13

0.12 0.17

0.10 0.14

0.10 0.14

0.10 0.14

0.13 0.16

0.09 0.14

O.OS 0.10

0.10 0.15

0.18 0.25

0.22 0.32

0.10 0.14

0.17 0.24

0.19 0.28

0.22 0.32

021 0.31

0.19 0.27

0.21 0.30

0.09 0.13

0.10 0.14

O.n 016

0.03 0.05

0.08 0.11

0.10 0.14

0-10 0.15

0.11 0.17

0.06 0.12

0.07 0.10

0.06 0.09

0.10 0.15

0.06 0.12

0.09 0.12

0.10 0.15

0.10 0.15

0.09 0.13

0.09 0.13

009 0.13

0.11 0.16

0.09 0.13

0.11 0.15

0.02 0.03

0.09 0.13

0.11 0.15

053 0.77

0.34 0.46

O.IS 0.21

0.21 0.30

0.50 0.72

0.31 045

0.15 0.21

0.08 0.12

0.04 0.05

0.08 0.12

0.07 0.11

0.06 0.08

0.07 0.11

0.07 0.11

0.09 0.12

0.08 0.11

0.09 0.12

0.13 0.18

0.14 0.20

0-14 0.20

0.18 026

0.26 0.38

0.24 0.34

021 0.30

0.19 028

0.07 0.10

0.07 0.11

0.10 0.14

0.11 0.16

0.09 0.13

0.06 0.09

0.13 0.19

0.18 026

0.11 0.16

0.16 0.22

0.17 0.25

0.13 0.19

0.04 0.06

0.06 0.09

0 44 0 .&4

0.11 0.16

021 0.28

0.20 0.26

0.20 027

0.16 0.22

0.23 0.31

0.16 0.25

0.20 0.28

0.28 0.36

0.09 0.13

0.15 021

0.05 007

0.12 0.16

0.16 0.23

0 IS 0.21

0.12 0.16

0.25 0.34

025 0.35

0.19 0.26

0.24 0.33

0-20 0.27

019 0.27

0.20 028

026 0.36

0.19 0.26

0.18 0.26

0.21 0.30

0.36 0.50

0.44 0.62

0.20 0.26

0.34 0.47

0.39 0.6S

0.45 0.63

0.43 0.60

0.39 D.54

0.42 0.59

0.19 026

0.20 0.27

0.22 0.31

0.07 0.09

0.16 022

0.20 0.28

0.21 0.29

0 23 0.32

0.17 0.23

0.14 0.19

013 0.17

0.20 0.28

0.16 0.23

0.17 0.24

0.21 0.29

0-21 0-28

0.18 026

0.19 0.26

0.18 025

022 0.30

0.18 0.25

021 0.30

0.05 0.06

0.18 0.25

022 0.30

1.08 1.50

0.63 0.94

0.29 0.41

0.42 0.59

1.01 1.40

0.64 0.83

029 0.41

0.16 022

O.Oa 0.10

0.16 0.23

0-15 0.21

0.12 0.16

015 0.21

0.15 021

0.17 0.24

0.16 022

0.17 0.24

0.26 0.36

0.29 0.40

0.28 0.39

037 031

033 0.73

0.48 0.67

0.42 D.53

0.39 035

0.14 0.20

0.15 0.21

020 0.27

0.23 031

0.18 0.25

0.13 0.1B

027 038

0.37 031

022 0.30

0.32 0.44

0.35 0.49

0.26 037

0 09 0.12

0.13 0-18

O.SO 1 .25

0.22 0.29

039 0.53

0.38 0.52

0.37 0.50

0.30 0.40

0.43 0.53

0.34 0.46

0.38 0.52

0.53 0.71

0.16 0.24

0.29 0.39

0.10 0.14

0.22 0.30

0.31 0.42

0.29 0.39

0.22 0.30

0.47 0.63

0.47 0.64

0.35 0.48

0.45 0.61

0.37 O.SO

0.37 0-49

0.38 0.51

0.49 0.66

0.36 0.49

0.40 0.49

0.42 0.56

0.68 0.92

0.84 1.14

0.36 0.52

0.64 0.86

0.7S 1.01

0.86 1.16

0.82 1.10

0.73 0.59

0.60 1.08

0.35 0,47

0.37 0.60

0.43 038

0.13 0.17

0.30 0.41

0.38 031

0.39 0.63

0.44 0.60

0.31 0.42

0.26 0.35

0.24 0.32

0.39 0.52

0.31 0.42

0.33 0.44

0.40 0.S4

0.39 0.53

0.35 0.47

0.36 0.49

034 0.46

0.42 0.55

0.34 0.46

0.41 0.55

0.09 0.12

0.34 0.46

0.41 0.56

2.05 2.77

1.29 1.74

0.56 0.75

0.60 1.08

1.91 2.58

1.21 1.63

0.56 0.75

0.31 0.41

0.14 0.19

0.31 0.42

0.29 039

032 0.30

039 0.39

0.29 0.39

033 0.45

0.30 0.41

033 0.45

0.49 0.66

0.54 0-73

034 0.72

0.70 0-64

1.00 1.36

D-Sl 1.23

0-79 1.07

0.75 1.01

037 036

0.29 039

0-37 030

0.43 0.55

0.34 0.46

0.25 0.33

032 0-70

0.70 0.65

0.42 0 35

0.60 D.B1

0.67 030

030 0-68

0.16 032

034 032

1.71 2.30

9

0 39 0.70 0.69 0.67 0.53 0.77 0.61 0.68 0.G4 0.31 0.51 0.16 0.39 0.56 0.52 0.39 0.83

0.67

0.65

0.68

0.83

0.65

0.59

0.72

1.22

1.S1

0.68

I.IS

1.34

1.54

1.46

1.31

1-44

0.63

0.67

0.76

033

0.SS

0.68

0.70

0.79

0-47

0.43

0.69

0.S6

0.59

0.72

0.70

0.53

0.84

0.61

0.74

0.62

0.73

0.16

0.61

0. 74 3.66 2.31 1.00 1.44

3.42 2.16

1. C-0 035 0.26 0.55 032 0.40 031 0.51 039 0.S4 939 D-S7 037 0-95

1.25 1.79 1.64

1.42 1.34 0.48 031 0.67 0.77 •0-61 0.44 ■0.93

1.26 0.73 1.07 1.20 030 0.29 0.43 3.06

10

0.51

0.91

0.90

0.87

0.69

1,01

0.80

0.89

1.23

0.41

0.67

0.24

0.52

0.73

0.67

0.52

1.09

1.11

0.83

1.05

0.87

0.86

0.89

1.1S

0.85

0.62

O.SO

1.59

1.97

0.89

1.50

1.76

2.01

1.91

1,72

1.88

0.82

0.88

1.00

0.30

0.71

0-89

031

1.04

0.74

0.61

0.56

0.91

0.94 031 0.82 0.84 0.80 0.S7 0.81 0.85 0.21 0.8 D 0.97 4.81 3.02 1.31 1.88 4.47 2.63 1.31

0.72

0.68

032

0.67

0.67

0.78

0.71

0.78

1.14 1.27 1.25 1.63 2.34

2.14 1.86 1.76

0.87

0.87

1.01

0.80

1.21

1.64

0.58

1.40

1.56

1.17

036

036

4.00

Day by day room breathing zone maximum NH3 1 2 3 4 5 6 7 6 "

0.07 0.09 0.09 0.09 0 06 0.08 0.19 0.12 0.14 0.08 0.12 0.12 0.16 0.11 0.16 0.13 0.09 0.09 0.12 0.09 0.1C 0.11 0 09 0.22 0.12 0.06 0.12 0.16 0.14 0.13 0.16 0.18 0.22 0.17 0.15 0.16 0 09 0.10 0.13 0.04 0.07 0.10 0.09 0.12 0.13 0.10 0.09 0.16 0.13 0.10 0.12 0.10 0.09 0.15 0.13 0.08 0.15 0.13 0.05 0.09 0.16 0.47 0.34 0.17 0.17 0.43 0.32 0.14 0.14 0.06 0.16 0.13 0.08 0.13 0.13 0.12 0.10 0.11 0.10 0.11 0.14 0.17 031 030 0.23 0.19 0.07 0.04 0.11 0.15 0.10 0.06 0.19 0.17 0.15 0.15 0.15 0.17 0.06 0.10 0.41

0.14

0.18

0.17

0.17

0.16

0.16

0.36

0.23

0.28

0.16

0.23

033

0.28

0.22

0.31

0.25

0.18

0.18

0.22

0.18

0.19

0.22

0.18

0.43

0.24

0.15

0.24

0.31

0.27

0.25

0.32

0.34

0.44

0.32

039

0.31

0.18

030

035 0.09 0.14 0.19 0.17 0.24 0.25 0.20 0.18 0.31 0.25

030 033 0.37 0.17 0.29 0.24 0.16 0.28

036 0.09 0.18 0.31 0.91 0.66 0.33 0.32 0.83 0.63 0.26 036 0.12

031 036 0.15 035 035 0.24 0.19 031 0.20

031 038 0.34 0.41 0.38 0.4S 0.38 0.14 0.09

032 0.28 0.19 0.15 0-37 0.33 038 0.28 030 D.34 0.12 D.19 O.BD

0.21

0.28

0.27

036 0.24 0.25 0.5B 0.35 0.43 0.25 0.35 0.36 0.44 0.34 0.48 0.39 0.26 0.28 0.35 0.27 0.29 0.34 0.28 0.67 0.36 0.23

037 0.48 0.42 0.39 0.49 0.53 0.67 0.50 0.45 0.47 0.28 0.31 0.39 0.13 0.22 0.30

036 0.37 0.38 0.31 0.27 0.47 0.38 0.31 0.36

037 036 0.44 0.38 0.25 0-44 0.40 0.14 0.27 0.48 1.40 1.01

031 030 1.28 0.37 0.41 0.41 0.19 0.48 0.40 0.23 0.39 039 0.37 030 0.33

030

032 0-43 032 0.53

038 0.39 038 0.21 0.13 0.34 0.44 0.29 0.24 036

031 0.44 0.44 0.46

032 0.19 0-30 1.24

0.31

0.41

0.38

0.36

0.35

0.35

0.83

0.51

0.62

0.36

0.50

0.52

0.63

0.49

0.89

0.S7

0.41

0.41

0.50

0.39

0.42

0.49

0.41

0.86

0.S2

0.45

0.57

0.69

0.60

0.57

0.71

0.76

0.97

0.71

0.S5

0.58

0.40

0.44

038

0.19

0.31

0.43

0.37

0-53

0.55

0.45

0.40

0.58

035 0.44

032 0.32

038 0.54 034 0.35 0.53 037 0.20 0.39 0.70 2.02 1.46 0.73 0.72 1.84 1.40

039 039 037 0.59 037 0.33

036 036

033 0.43 0.48 0.44 0.47 0-S3 0-75 0.91

C. 35 1.00 0-W 0.30 0.19 0.49 0-S3 0.41

034 0.51 0.74

D. S3 O.S3 0..S6 0.73 0.2B 0.43 1.78

0.43

0.57

0.54

0.54

0.49

O.SO

1.17

0.71

0.80

O.SO

0.70

0.73

0.89

0.70

0.97

0.30

0.57

0.57

0.70

0.55

0.59

0.59

0.57

1.35

0.74

0.76

0.83

0.97

0.85

O.SO

1.00

1.07

137

1.01

0.92

0.96

0.56

0.62

0.79

0.27

0.44

050

0.52

0.75

0.78

0.83

036 0.9S 0.78 0.62 0.72 1.15 0.53 0.90 0.76 030 0 88 0.30 039 0.5S 0.9B 2.84 2.06 1.02 1.02 2.59 1.97 G.83 0.82 0.39 0.97 O.SO 0.46 0.73 C.78 0.75 0.60

037 0-32 0.35 0-58

1.05 1.27 1.20 1.41 1.18 0.42 0.27 0 59 0-5B D3B 0.48 1.14 1.04 C'.SS 039 0.93

1.06 0-39 0-51 2.S1

0.50

0.79

0.75

0.74

0.67

0.69

1.63 0.99 1.21 0.69 0.9S 1.01

1.23 0.96

1.34 1.10 0.79 O.SO 0.97 0.76 0.S2 0.95 0.79 1.87 1.02

1.13 1.18

1.35 1.17 1.10 1 38

1.48 1.00 1.39 1.26

1.33 0.78 0.86 1.09 0.37 0.51 0.83 0.72 1.04 1.0B 0.66 0.77 1.32 1.08 0.87 1.00

1.59 0.74

1.24 1.06 0.59 1.22 1.11 0.40 0.76

1.36

3.94 2.85 1.42 1.41

3.59 2.73 1.15

1.14 034

1.34 1.11 0-B4 1.08 1.08 1.04 0.54

033 0.56 031 1.22

1.46 1.77 1,67

1.95

1.64 039 0-38 0.95 1.22 D.3D 0 55 1.50 1.44 1.23 1.23 1.29

1.46

034 0-54

3.48

0.83

1.08

1.02

1.02

0.92

0.94

2.23

1.35

1.66

0.95

1,34

1.39 1.69

1.32 1.63

1.51 1.08

1.09

1.33

1.05 1,12 1.30

1.06

2.56

1.40 1.71 1.65 1.84 1.60 1.61

1.69 2.02 2.60 1.91

1.75 1.62 1.07 1.18 1.50 031 0.84 1.14 0-98 1.42 1.48 1.20 1.06 1.81 1.48 1.19 1.37 2.18 1.01

1.70

1.45 0.94 1.68

1.52 0-S4 1.05 1.86 5.39

3.90 1.94 1.93

4.91 3.74

1.57 1.56 0.73 1.83 1.52 0.37

1.46 1.48

1.42 1.14

1.27

1.17

1.24

1.67 2.00

2.42

2.28

2.67

2.24 0.31 0.S1 1.30

1.68

1.10 0-90

2.17 1.97 1.69 1.68

1.76 2.00 0.74 1.16

4.76

1.11

1.46

1.38

1.37

1.24

1.27

3.00 1.82

2.24

1.28 1.80

1.87

2.27 1.78 2.48 2.04

1.46

1.47 1.60 1.41 1.61 1.75

1.46

3.46

1.88 2,12 2.18

2.48 2.16

2.04

2.55 2.73 3.50

2.57

2.36

2.46 1.44

1.59 2.02 0.59 1.13

1.53 1.32 1.92 1.99 1,62

1.43

2.44

2.00

1.60 1.85

2.94

1.36 2.30

1.95

1.27 2.26

2.05 0.73

1.41 2.61

7.27

5.26 2.62 2.60 6.63

5.05 2.12 2,11 0.99

2.47

2.05 1,18 2.00 2.00

1.91

1.54 1.71

1.58 1.68

2.25

2.69

3.26 3.07 3.60 3.03 1.09 0-99 1.75

2.26

1.48 1.22

2.92 2.66

2.27

2.27

2.38

2.70 HjOD

1.56

6.42

9

1.40

1.94

1.83

1.83

1.65

1.69 3.99

2.42

2.98

1.70 2.30 2.48

3.02 2.36

3.29

2.70

1.94

1.95

2.39

1.87 2.00

2.33 1.94

4.59

2.60 2.52 2.81

3.30 2.68

2.71

3.39

3.63

4.65

3.42 3.13 3.26 1,92 2.12 2.68 0.92 1.50

2.03 1.76 2.55

2.64 2.15

1.89 3.25

2.65 2.12

2.46

3.90 1,81

3.05

2.59

1.69 3.00 2.73 0.97

1.87

3.33

9.65

6.99

3.46 3.45 8.81

6.70 2.61

2.60

1.31 3.28 2.73

1.57

2.66 2.66 2.54

2.05 2 28 2.10 2.23

2.99

3.58

4.33 4.08 4.78 4-02 1.44 0.92

3.00 1.97 1.62 3.86 3,63 3.02

3.01 3.16 3.59 1.33 2.07 8.53

10

1.93

2.53 2.39

2.39 2.16 2.21

5.21 3.16

3.89

2.22 3.13

3.26 3.95

3.09

4.30

3.53

2.54

2.55

3.12

2.45 2.62

3.04 2 54 6.00

3.27 2.66 3.61

4.31

3.76

3.54 4.43

4.74 6.08

4.47

4.09

4.27

2.51

2.77

3.61 1.20

1.97 2.66 2.30

3.33

3.46 2.81

2.48

4.24

3.47

2.78 3.22

5.10

2.36

3.98

3.39 2.21 3.92 3.57

1.27

2.45

4.36

12.61

9.13

4.55

4.51

11.51 8.76

3.68

3.66

1.72 4.29

3.56

2.05

3.48

3.46

3.32

2.68

2.98

2.74

2.91

3.91

4.67

5.67

5.34

6.25

5.26

1.89 1.21 3.04

3.92

2.67 2.12

5.06 4.62 3.95

3.94

4.13 4.69

1.73 2.71 11.15

Table 2.03 Room NH3 Concentrations for 10 Days with Increased Cage Temperature

Volume II -36

Ventilation Design Handbook on Animal Research Facilities Using Static Microisolators

Cosa Name 1

Basecaso 1.12

Case 02 0.89

Case 03 0.82

Case 04 0.89

Case 05 0.91

Case 06 0.86

Case 07 1.21

Case 08 0.97

Case 09 0.90

Case 10 1.10

Case 11 1.10

Cose 12 1.15

Case 13 1.30

Case 14 1.16

Case 15 1.10

Case 16 1.16

Case 17 0.94

Cose 16 0.93

Cose 19 0.90

Case 20 0 66

Case 21 0.94

Cose 22 0.67

Case 23 0.66

Case 24 0.98

Case 25 1.56

Case 26 2.12

Case 27 1.66

Case 28 0.91

Case 29 0 92

Case 30 0.87

Case 31 1.01

Case 32 0.91

Case 33 1.03

Case 34 0.99

Case 35 0.92

Case 36 0.98

Case 37 0.89

Case 36 0.92

Case 39 0.96

Case 40 1.03

Case 41 0.86

Cose 42 0.90

Cose 43 0.97

Case44 1.10

Case 45 1.00

Case 46 1.01

Cose 47 1.02

Case 46 109

Case 49 1.05

Case 60 0.96

Case 51 1.06

Case 52 0.87

Case 53 1.16

Case 64 0.97

Case 55 1.06

Case 66 0 98

Cose 67 0.96

Cose 56 1.03

Case 59 1.12

Cose 60 1 .22

Cose 61 1.06

Cose 62 1.21

Case 63 1.06

Case 64 0.94

Case 65 1.04

Cose 66 1.21

Case 67 1.11

Case 66 0.96

Cose 69 0.91

Case 70 1.02

Case 71 1.02

Cose 72 0.93

Cose 73 1.02

Case 74 0.98

Case 75 0.96

Cose 76 0.97

Cose 77 0.96

Cose 70 1.00

Case 79 0.86

Case 80 0.89

Cose 61 0.91

Cose 02 0.87

Case 83 0.91

Case 84 0.96

Cose 65 1.00

Cose 86 1.00

Case 87 0.72

Case 68 0.81

Case 89 0.85

Case 90 0.87

Casa 91 0.69

Cose 92 0.66

Case 93 0.95

Case 94 0.91

Cose 95 0.05

Case 96 1.02

Case 97 0.60

Case 96 1.02

Case 99 103

Cose 100 0.90

Case 101 1.26

2

2.18

1.72

1.59

1.73 1.76 1.67

2.35

1.89

1.75 2.14 2.14

2.23 2.52

2.24 2.14 2.24 1.84 1.81

1.76 1.66 1.83 1.69 1.67

1.91

3.04 4 02 3.21

1.77 1.79

1.69

1.96 1.77 2.00

1.92

1.70

1.90

1.74

1.60

1.87 2.00

1.71

1.76 1.86 2.14

1.95

1.97

1.98

2.12

2.05 1.86

2.07 1.69 2.24 1.89

2.06

1.91

1.88 2.01 2.19

2.36

2.07 2.35 2.11 1.63 2.03 2.35 2.17 1.66

1.77

1.96

1.99 1.81 1.99 1.91

1.90

1.94 1.67 1.73 1.76 1.69 1.78 1.66

1.95 1.94 1.41 1.67

1.73

1.66

1.84

1,70

1.84

1.98

1.75

1.98

2.01

1.75

2,43

Doy fay day cage average NH3

3 4 5 6 7 8 9 10

3.37

2.66

2.45 2.67

2.72 2.58

3.62

2.92

2.69 3.31

3.30

3.45

3.69

3.46

3.31 3.45

2.63 2,79

2.69 2.56 2.62 2 60

2.56

2.94

4.69 6.20

4.95

2.73 2.76 2.60

3.02

2.73 3.06

2.96

2.76

2.93 2 68

2.77 2.66 3.06 264

2.70 2.90 3.31 3.01

3.03

3.06 3.26 3.16

2.67 3.19 2.60 3 45 2 92

3.16

2.94

2.69 3.10 3.37

3.67 3.19

3.63 3.26 2.62 3.12 3.62 3.34

2.07

2.73 3.05

3.07

2.70

3.07 2 94

2.07 2 90

2.03 2.99

2.57 2.66 2.72 2.60

2.74

2.89 3.01

2.90

2.17 2 42 2.65 2.61 2 67 2.55

2.64

2.74 2.64

3.05

2.69

3.05 3.10

2.70

3.74

4.85 3.83

3.53 3.64

3.02 3.72 5.22

4.21 3.66 4,77

4.76

4.07

5.60 4 98

4.77

4.98

4.08

4.03

3.88 3.72 4.07

3.75

3.71 4.24

6.77 11.92

7.53 3.93 3.97

3.76 4.36

3.93 4 44 4 26

3.06

4.22

3.86

3.99

4.16 4 44

3.60

3.69

4.17

4.76 4 34 4 37 4.41

4.72 4 55 4.14

4.60

3.75 4 97 4.20 4 50 4 24

4.17 4.47

4.86 5.29

4.69

6.23 4.68

4.07 4.S0 521 4.62

4.13

3.94 4.40 4.43

4.03 4 42 4 24

4.14

4.18 4 23 4 32

3.70 3.64 3.92

3.76

3 96 4,17

4 34 4.31 3.13 3.49

3.66

3.76 3.64

3.67 4.10

3.95 4.10

4.40

3 88

4 40 4 46

3.89

5.40

6.63

5.39

4.96

6.40

5.51

5.22 7.34 5.92 5.46 6.70 6.70

6.96 7,68 7.01 6.70

7.00 5.74

6.67

5.46

5.24

5.72

6.27

6.22

5.96

9.51 20.30 11.08

5.53

5.58

5.27

6.13

6.53

6.24

6.00

5.67 5 94 6-43 6.62

5.65

6.25

5 35

5.47

5.07

6 70 6.10

6.14 6.20 664

6.41 5.82

6.47

5.27 7.00 S.91

6.45 5.96 6 67 6.29

6.64

7.45

6.46 7,36 6.S6

5.72

6.33

7.33 6,78 6.81 6.64 6.16 6 22

5.66 6.22 5.96 5.62 6.87 5.95

6.07 6.21

5.40

5.51

5.27 S.5S 5.86 6.11 6.06

4 40 4.90 5.17 6.20

5.41

5.16

5.76

6.66

5.76 6.19 5,40

6.16

6.26

5 47

7.59

9 47 7.47 6.86 7.49 7.64

7.24 10,18 6.21 7.57

9.29

9.20

9.66

10.93

9.72 0.29 0.71

7.98 7.86

7.57

7.26

7.93

7.30

7.24

6.26 13.10 30.25

15.62

7.67 7.74

7.31 ,6.60

7.M

8.66

8.31

7.72

6.24 7.52 7.79 0.11 6.66

7.41

7.60

8.14 0.29

6.46

6.62

6.60

9.21 8.66 6.07 6.97

7.30

9.70 8.20 8.04 8.27

6.14 0.73

9.46 10.32 6.96 10.20

9.13 7.93 6.76

10.17

9.40 6.06

7.60

6.58

6.63 7,66 8.62 8.27 6.07

8.15 8.26

8.42

7.22 7.49

7.64

7.31

7.70

8.13

8.47

8.41

6.10

6.60

7.17 7.34 7.60 7.10

7.99

7.71 7.90

8.58

7.57

8.57 8,70 7,69 10.53

12.96 10.22 9.41 10.26 10.46 9.92

13.94

11.23 10.38 12.72

12.71

13.25 14 95 13.30

12.72

13.29 10.69 10,75

10.36

9.94 10 65 10.00 6.91

11.30

18.05

45.71 21 95 10.50

10.59 10,01

11.63 10.46

11.65

11.36

10.67

11.27

10.30

10.66 11,09 11.66 10.16 10 37 11.14

12.71 11.56 11.66 11.77

12.60 12.16

11.05

12.27 10.00

13.26 11.22

12.24 11.32

11.13

11.94 12.07

14.13

12.26

13.96 12.40 10.65 12.02

13.01

12.67

11.03 10.61

11.74

11.01

10.74 11.60

11.31

11.04 11,16 11.29 11,52 9.66

10.24 10.45 10.00

10.64 11,12 11.60 11.61

6.35 9.30 9.62

10.04 10,26 9.79 10.93

10.65 10.03

11.74

10.35

11.73 11.01 10.38 14 40

17.40

13.79 12.70

13.64

14.11 13.37

18.80

15.15

13.97

17.16

17.15 17.86

20.17 17.94

17.16

17.92

14.69

14.50 13.07

13.41

14.64

13 48

13.37

15.25

24.35

56.46 28.91

14.16

14.26

13.50

16.69

14.16

16.98

15.35

14.26

15.20

13.69

14.38

14.96 16.00 13.69

13.09 15.02 17.15

15.62 15,73

15.68

17.00

16.40

14.90 16.56

13.46

17.91 15.14

16.51

15.27 15.02

16.11

17.50

19.06

16.54

18.63

16.65

14 64

16.21 18.77

17.36 14.60

14.17

15.63

16.93

14.40

15.92

15.26

14.69 16.04 15.23

15.54 13.33

13.82

14.10

13.50

14.22

15.00 15.63 15.62

11.26

12.65 13.26

13.65

13.84 13.21

14.75

14.23

14.75

16.84

13.97

16.83

16.07

14.01 19.43

23 21

18.31

16.87

18 38

18.74

17.76

24.97 20.12

18.55

22.78

22 78

23.75

26.79

23 62

22.70

23.80

19.52 19.26

18.56

17.81 19.44

17.91

17.76

20.25

32.35 67.24

37.36

18.81

18.97

17.03 20.84 10,79

21.23

20 39

18.03 20.19 18.46 10.10

19.88

21.24 16.18 16.58

19.95

22.78 20.74 20.60

21 09

22.67

21.78

19.70 21.99

17.01

23.70 20.10

21.03

20.26

10.95 21.40

23.24

26.32

21.96

26.01 22.38

19 44

21.53

24.92 23.05

19.77 16.82

21.03 21.10

10.25 21.14 20.27

10.70

19.97 20.23

20.64

17.71

16.35

10.72

17.92 18 88

19.03

20.76

20 62 14 96

16.67 17.50 17.90

18.36

17.54 19.69 18 00 19.69

21.04

16.65 21 02 21.34 18.61 25.81

30.34

23.94

22.05

24.02 24.50 23.22

32.63

26.30 24 25 29.78

29.77

31.04

35.02

31.14

29.78

31.11

25.61 26.16

24.26

23.28 26.42 23.41 23.22 26.47

42.28

71.62

46.66

24.60 24.80 23 44

27.26 24.57

27.75 26 65

24.76

26.40

24.11

24.96 26.68

27.77

23.76 24.20 26.08

29.77

27.11

27.31 27.56

26.61

26.47 25,87 28.75

23.41

31.09

26.28

28.67 26.61 26 06

27.97 30.39

33.09 28,71

32.60

29.25

25.42

26.15 32.se

30.13

26.64

24.61

27.49 27.66

25.16

27.63

26.49 25.85

26.11 26 44 26.08 23.15 23.99

24.47

23.43

24.68

26.05

27.14 26.95 19.55

21.78 23.00 23-52

24.02 22.93

25.61 24.70

26.61 27,61

24.25

27.48 27.89

24.32 33.74

1 2 3

1.63 3.17 4.89

1.26 2.51 3.87

1.34 2.61 4.03

1.19 2.32 3.58

1.21 2.36 3,64

1.38 2.68 4.13

1.76 3,43 6,28

1.37 2.67 4.12

1.52 2.67 4.57

1.67 3,24 5.00

1.51 2,04 4.53

1.57 3.06 4 72

1.56 3.04 4.66

1.36 2.65 4.09

1.67 3.24 5.00

1.63 3.16 4.68

1.26 2.49 3 64

1.27 2.47 3.80

1.22 2.36 3.67

1.22 2.38 3,67

1.33 2.69 3.99

1.36 2.66 4.09

1.33 2.56 3.66

1.57 3.06 4 72

2.05 3.96 6.15

2.58 4.90 7.65

2.36 4.61 7.11

1.41 2.74 4.22

1.33 2.59 3.96

1.34 2.61 4.02

1.48 2.07 4.43

1.45 2.62 4.35

1.63 3.17 4.89

1.40 2.72 4,20

1.40 2.72 4.20

1.35 2.63 4.05

1.17 2.27 3.60

1.39 2.70 4.16

1.40 2.72 4.10

1.45 2.81 4.34

1,24 2.41 3.71

1.32 2.57 3.96

1.26 2.46 3.79

1.42 2.77 4.26

1.36 2.71 4.10

1.43 2.78 4,28

1.46 2.84 4.38

1.41 2.75 4.24

1.40 2.72 4.19

1.35 2.63 4.05

1.41 2.74 4.23

1.36 2.66 4.09

1.47 2.86 4.41

1.36 2.71 4.18

1.36 2.66 4.00

1.34 2.60 4.01

1.43 2.77 4.26

1.49 2.00 4.48

1.42 2.77 4.27

1.52 2.96 4.67

1.47 2.86 4.41

1.76 3.43 5.20

1.77 3.44 5.31

1.36 2.65 4.09

1.64 3.19 4.91

1.80 3.61 S.41

1.77 3.45 5.31

1.61 2.63 4.52

1.34 2.61 4.03

1.43 2,77 4.28

1.40 2.72 4 10

1.36 2.69 4 16

1.41 2,73 4,22

1.37 2.66 4.11

1.36 2.65 4 09

1.27 2.46 3.80

1.38 2.68 4.14

1.37 2-66 4.10

1.22 2,38 3.67

1.40 2.72 4.19

1.41 2.75 4.24

1.45 2. 63 4.36

1.67 3.06 4.72

1.54 3.00 4.62

1.48 2.67 4.43

1.69 3.29 5.07

1.37 2.67 4.12

1.31 2.55 3.03

1.35 2,63 4,05

1.31 2.54 3.62

1.37 2.60 4.12

1.26 2.43 3.76

1.45 2.81 4.34

1-42 2.77 4.27

1.45 2.81 4.34

1.44 2.60 4.31

1.50 2.62 4.60

1.41 2.74 4.23

1.54 3.00 4 62

1.72 3,35 5.16

1.63 3.66 6.50

Day by day cage maximum NH3

4 5 6 7 6 9 10*

7.05

5.57

5,81

5.16

5.25

6.95

7.61 5.93

6.59 7.20

6.53

6.60

6.75

5.69 7.20

7.03

5.54 5.46 5.29

5.29

5.75 5.60

5.74 6.80 6.86 14.51 10.62 6.08

5.75

5.79 6.36 6,27

7.05

6.05

e.os

5-63

6.05

6.00

6.04

6.25

5.35

5.70 5.46

6.15 6.03 6.18 6.31 6.11 6.03 6.63

6.09 5.90 6 36 6.02 6.00 5.78

6.17

6.45

6.16

6.59

6.35

7.62

7.65

5.89

7.08

7.80

7.66 6.52

5.81 6.16 6.03 6.99

6.08

6.92

5.90

5.46 6.97 6.00

5.30

6.05

6.12

6.26

6.60

8.67 6.39

7.31

5.63 5.66

5.65

6.66

5.96 6.41

6.25 6.15

6.26 6 21 6.49

6.10 6.66 7.43

7.92

9.91

7.04

6.16

7.26 7.38 8.37

10.71 8.34

9.27 10.13 9.18 9.56

9.46 6.26 10.13

6.69

7.79

7.70 7.44

7.44

e.oe

8.30

8.07

9.56

12.46

24.70 15.90 6.66 8.06 8.15

8.96 6.82 9.61

6.50

8.51 6.20 7.10 8.43 6.49

6.79

7.53 6.02

7.66 8 64

6.48

8.66

8 67

6.59

6.49 8.20 0,57

8.30 8.94 8 47

6.30 8.13

8.67

9.07 6.66

9.27 6.03

10.72

10.76 8 28

9.96 10,97

10.77

9.17

8.17

6.67

8.49 8.42

6.54

8.33 8.29 7.70

8.36

8 30

7.45

8.50

8.60

6.64 0 56

9.37 6.66 10.28

6.34 7,06 8.22

7.64

6.36 7.60 0.79

8.65 8-79 8.74

9 13

8.56

9.36

10.46 11.14

13.74

10.87

11.32 10.07

10.23 11.60

14.85

11.57

12.86 14.04

12.73 13.26

13.16 11.49

14.04

13.71 10.81 10.68

10.32

10.32 11.21 11.51

11.16

13.26 17.28

36.81

22.45 11.86 11.21

11.30

12.45

12.23

13.74

11.76 11.80 11.38 6.84 11.69

11.77

12.19 10.44 11.12

10.65 11.98

11.76

12.04

12.30

11.91

11, n

11.38 11.86 11.51 12.36

11.74 11.61

11.27 12.02

12.58 12.01

12.85

12.39

14.86

14.92 11 48

13.81 16.21

14.63

12.71

11.33 12.02

11.77 11,60 11.85 11.55 11.60

10.66

11.63 11.61

10.33 11.79

11.93 12.26 13.26

13.00

12.46 14,25 11,57

11.04

11.40

11.01 11.69

10.65

12.19 11.90 12.16 12.12

12.65 11.89 12.96 14.60 15 45

16.81

14.87

15.50

13.78

14.00

15.88

20.32

15.63

17.60 19.22

17.43

16.15

18.01 15,72

19.22

18.76

14.79

14.62

14.13

14.12 15.34

15.76

15.32

16.14

23.64

55.62

31.65 16.24

15.34

15.47

17.04

16.74 18.81 16.14

16.16

15.57

13.47 16.00 16.11 16.68

14.26

15.22

14.58

16.40

16.09

10.48 16.64 16.29

16.10

16.57

16.26

15.75 16.96 16,07

15.75

15.43 16.46

17.22

16.44

17.58 16.95

20.34

20.42 15.71

18.60 20.82

20.43

17.40

16.50

10.45 16.10 16.98 16 22

15.80 16.74 14 62 15.02

15.76

14.13

16.14

16.33

16.77

18.14 17.79

17.05

19.50 16.84 15.10 15.60 16,07 16-87

14.43 16.69

16.41 16.69

16.58 17,32 16.26 17.76 19 64

21.14

25.37

20.06

20.90

16.59

16.69 21.42 27.41

21.36 23.74 25.92 23.51

24.48

24.30 21.21 25.92

25.31

19.05

19.72

19.06

19.05

20.69 21.24

20.67

24.47

31.89

68.72

41.67

21.90

20.70 20.86

22.90

22.59

25.37 21.77 21.79 21.00 18.17 21.58

21.73

22.50 19.28 20.62

19.67 22.12

21.71

22.23

22.72 21.66

21.72 21.00 21.94 21-24 22.86 21.66

21.24 20.81 22.20

23.23 22.16

23.72

22.87 27.44

27.55 21.20

25.49

28.06

27.67

23.47 20.61 22.19

21.72

21.56

21.87

21.32

21.23

19.72

21.47

21.25 19.07

21.77

22.03 22.63

24.48

24.00

23.00 26.31

21.36

20.37

21.04

20.33 21.40 19.47 22.61 22.14

22.51

22.37 23.36

21.96

23.96

26.77

28.51

33.70

26.65

27.76 24 69 26.09

26.44 36.40 28,37 31,63 34.43

31.22

32.52 32,27 26.16

34.43

33.62

26.49

26.19

25.31

26.30 27.46 28.21

27.46

32.50

42.36

81.62 53.72 26.09

27.49

27.71

30.63

30.00

33.69 26.61

26.94

27.89

24.13 28.67 28.87

29.86

25.60 27,26 26.12

26.36

28.63

29.63

30.17

29.19

28.85

27.69

29.14 28.21

30.39 20.79 26.21

27.64

29.46

30.85

29.45

31.60

30.37

36.44

36.50

28.15

33.66

37.30

36.61

31.17

27.77 29.48 28 85 28.63

29.05

28.32

26.20 26.19

26.52

26.23

25.32 28.91 29.25

30.05

32.51

31.87

30.54

34.94

28.37

27.00

27.95 27.00 28.43

25.66

29.90

29.40 29.90

29.71 31,03

29.17 31.82

35.55

37.67

44.05 34.63

36.29 32.27

32.80

37.18

47.59

37.00

41.21

45.00

40.81 42.50

42.19

36.82

45.00 43.95

34.63 34.24 33.08

33.07

35.92

36.88

35.88

42.49

55.37

87.03

67.08 36.02

35.93

36.22 39.90 39.21

44.04

37.60

37.63

36.46 31.54

37.47 37.73

39.06

33.47

35.63

34.16 38.41

37.69

38.60 39.44

38.16

37.71

36.46

36.09

36.66

39.72

37.64 36.68 36.14 36 54 40.33

38.50

41.10

39.70

47.64

47.63 36.80 44.26

48.76

47.06

40.76

36.30 36.53

37.71

37.43 37.98

37.01 36.86

34.23 37.28 36.00

33.10 37.79

36.24 39 28

42.49

41.66

39.93

45.67 37.09

35.37 36.53 36.30

37.16

33.60 39.08

36.43

39.06

36.64 40.56 38.12 41.^

48.47

49.50

Table 2.04 Cage NH3 Concentrations for 10 Days with Increased Cage Temperature

Volume II - Section III - Rankings of Results of Whole Room Runs

Page II • 37

3. RANKINGS OF RESULTS OF WHOLE ROOM RUNS

3.1 Introduction

The following tables present rankings of the results of the CFD room simulations based on Room (scientists’ breathing zone) and cages mean values for CO2 and NH3 (day 4) concentrations, temperature, and relative humidity. The tables start with the case with the lowest value and list the rest in ascending order. The case number should be used to identify all details of the case.

The supply diffuser type, exhaust location, supply flow rate, and supply discharge temperature are given to provide a quick overview of the case.

Discussion of these tables can be found in volurpe I, section 3.3.

VoSume II -38

Ventilation Design Handbook on Animal Research Facilities Using Static Microisolators

Table 3.01 Ranking of Cases with respect to Room Temperature

Case Name	Supply Diffuser Type	Exhaust Location and Number	Supply ACH	Supply Temperature (°C)	Supply Temperature (°F)	Room Temp CC)	Room Temp CF)
Case 66	Lx)w Ind	Low	5	6.6	43.9	18.6	65.6
Case 62	Low Ind	Low	5	6.6	43.9	19.1	66.5
Case 53	Radial	Ceiling	15	18.8	65.8	19.8	67.6
Case 55	Low Ind	Ceiling	15	18.8	65.8	19.8	67.6
Case 54	Slot	Ceiling	15	18.8	65.8	19.8	67.6
Case 36	Low Ind	Ceiling	15	17.5	63.5	19.9	67.8
Case 35	Slot	Ceiling	15	17.5	63.5	20.0	67.9
Case 34	Radial	Ceiling	15	17.5	63.5	20.0	67.9
Case 07	Low Ind	Ceiling	15	18.8	65.8	20.0	67.9
Case 98	Low ind	Ceiling	15	17.5	63.5	20.0	68.0
Case 96	Radial	Ceiling	15	17.5	63.5	20.0	68.0
Case 97	Slot	Ceiling	15	17.5	63.5	20.0	68.0
Case 16	Radial	Ceiling	15	18.8	65.8	20.1	68.1
Case 46	Radial	Ceiling	15	18.8	65.8	20.2	68.3
Case 67	Low Ind	Low	10	14.8	58.6	20.2	68.3
Case 19	Radial	Ceiling	15	18.8	65.8	20.2	68.4
Case 18	Low Ind	Ceiling	15	18.8	65.8	20.2	68.4
Case 17	Slot	Ceiling	15	18.8	65.8	20.2	68.4
Case 94	Slot	Ceiling	15	17.5	63.5	20.2	68.4
Case 95	Low Ind	Ceiling	15	17.5	63.5	20.2	68.4
Case 43	Low Ind (rot 90°)	Ceiling	15	18.8	65.8	20.2	68.4
Case 47	Radial	Ceiling	15	18.8	65.8	20.3	68.5
Case 93	Radial	Ceiling	15	17.5	63.5	20.3	68.5
Case 45	Radial	Ceiling	15	18.8	65.8	20.3	68.5
Case 60	Low Ind	High	15	18.8	65.8	20.3	68.5
Basecase	Radial	Ceiling	15	18.8	65.8	20.3	68.5
Case 25	Radial	Ceiling	15	18.8	65.8	20.3	68.5
Case 27	Low Ind	Ceiling	15	18.8	65.8	20.3	68.6
Case 56	Radial	High	15	18.8	65.8	20.3	68.6
Case 59	Slot	Low	15	18.8	65.8	20.3	68.6
Case 63	Low Ind	Low	10	14.8	58.6	20.3	68.6
Case 90	Radial	Ceiling	15	18.8	65.8	20.4	68.7
Case 77	Slot	Ceiling	15	18.8	65.8	20.4	68.7
Case 76	Radial	Ceiling	15	18.8	65.8	20.4	68.7
Case 21	Low Ind	Ceiling	15	18.8	65.8	20.4	68.7
Case 92	Low Ind	Ceiling	15	18.8	65.8	20.4	68.7
Case 26	Slot	Ceiling	15	18.8	65.8	20.4	68.7
Case 78	Low Ind	Ceiling	15	18.8	65.8	20.4	68.7
Case 04	Slot	Ceiling	15	18.8	65.8	20.4	68.8
Case 44	Radial (rot 90°)	Ceiling	15	18.8	65.8	20.4	68.8
Case 91	Slot	Ceiling	15	18.8	65.8	20.5	68.8
Case 50	Radial	Ceiling	15	18.8	65.8	20.5	68.8
Case 52	Radial	Ceiling	15	18.8	65.8	20.5	68.8
Case 08	Low Ind	High	15	18.8	65.8	20.5	68.9
Case 42	Low Ind	Ceiling x4	15	18.8	65.8	20.5	68.9
Case 51	Radial	Ceiling	15	18.8	65.8	20.5	69.0
Case 05	Slot	High	15	18.8	65.8	20.5	69.0
Case 41	Slot	Ceiling x4	15	18.8	65.8	20.5	69.0
Case 40	Radial	Ceiling x4	15	18.8	65.8	20.5	69.0
Case 49	Radial	Ceiling	15	18.8	65.8	20.5	69.0
Case 20	Slot	Ceiling	15	18.8	65.8	20.6	69.0
Case 48	Radial	Ceiling	15	18.8	65.8	20.6	69.1
Case 02	Radial	High	15	18.8	65.8	20.7	69.2
1 Case 37	Radial	Ceiling/Low 50/50	15	18.8	65.8	20.7	69.3

Volume II - Section III - Rankings of Results of Whole Room Runs

Paee II - 39

Case Name	Supply Diffuser Type	Exhaust Location and Number	Supply ACH	Supply Temperature (°C)	Supply Temperature (°F)	Room Temp (°C)	Room Temp (°F)
Case 85	Radial	Low	15	17.5	63.5	20.7	69.3
Case 38	Slot	Ceiling/Low 50/50	15	18,8	65.8	20.8	69.4
Case 39	Lx)w Ind	Ceiling/Low 50/50	15	18.8	65.8	20.8	69.4
Case 65	Low Ind	Low	15	17.5	63.5	20.8	69.5
Case 13	Slot	Low	15	20.7	69.3	20.8	69.5
Case 10	Radial	Ceiling	15	20.7	69.3	20.9	69.7
Case 72	Radial	High x4/ Low x2	15	18.8	65.8	21,1	69.9
Case 74	Low Ind	High x4 / Low x2	15	18.8	65.8	21.1	70.0
Case 75	Radial	Ceiling/Low 50/50	15	18.8	65,8	21.1	70.0
Case 73	Slot	High x4 / Low x2	15	18.8	65.8	21.1	70.0
Case 14	Low Ind	Ceiling	15	20.7	69.3	21.1	70,0
Case 12	Slot	Ceiling	15	20.7	69.3	21.1	70.0
Case 64	Low Ind	Low	20	18.9	66.0	21.2	70.1
Case 86	Slot	Low	15	17.5	63.5	21.2	70.1
Case 29	Low Ind	Low	5	11	51.8	21.2	70.1
Case 15	Low Ind	Low	15	20.7	69.3	21.2	70.2
Case 68	Low Ind	Low	20	18,9	66.0	21.2	70.2
Case 22	Radial	Low	15	18.8	65.8	21.2	70.2
Case 69	Radial	High x4 / Low x4	15	18,8	65.8	21.3	70.3
Case 82	Radial	2 Door exhausts	15	18.8	65.8	21.3	70.3
Case 11	Radial	Low	15	20.7	69.3	21.3	70.4
Case 31	Radial	Low	15	17.5	63.5	21.4	70.5
Case 83	Slot	2 Door exhausts	15	17.5	63,5	21.4	70.6
Case 70	Slot	High x4 / Low x4	15	18.8	65.8	21.4	70.6
Case 84	Low Ind	2 Door exhausts	15	17.5	63.5	21.5	70.7
Case 71	Low Ind	High x4 / Low x4	15	18.8	65.8	21.6	70,8
Case 57	Radial	Low	15	18.8	65.8	21.7	71.1
Case 23	Slot	Low	15	18.8	65.8	21.8	71.2
Case 58	Slot	High	15	18.8	65.8	21.8	71.2
Case 79	Radial	2 Door exhausts	15	18.8	65.8	21.9	71.5
Case 32	Slot	Low	15	17.5	63.5	22.0	71.5
Case 33	Low Ind	Low	15	17.5	63.5	22.0	71.5
Case 61	Low Ind	Low	15	18.8	65.8	22.0	71.7
Case 03	Radial	Low	15	18.8	65.8	22.1	71.7
Case 80	Slot	2 Door exhausts	15	18.8	65.8	22.1	71.8
Case 24	Low Ind	Low	15	18.8	65.8	22. 1	71.8
Case 06	Slot	Low	15	18.8	65.8	22.3	72.1
Case 28	Low Ind	Low	10	16.8	62.2	22.3	72.1
Case 81	Low Ind	2 Door exhausts	15	18.8	65.8	22.5	72.4
Case 87	Radial	Low	15	19.2	66.6	22.5	72.4
Case 88	Slot	Low	15	19.2	66.6	22.6	72.6
Case 09	Low Ind	Low	15	18.8	65,8	22.6	72.6
Case 30	Low Ind	Low	20	19.8	67.6	22.6	72.6
Case 89	Low Ind	Low	15	19.2	66.6	22.9	73.3

Volume II -40

Ventilation Design Handbook on Animal Research Facilities Using Static Microisolators

Table 3.02 Ranking of Cases with respect to Cage Temperature

Case Name	Supply Diffuser Type	Exhaust Location and Number	Supply ACH	Supply Temperature (°0	Supply Temperature (°F)	Cage Temp rc)	Cage Temp (“F)
Case 66	Low Ind	Low	5	6,6	43.9	20.6	69.1
Case 62	Low Ind	Low	5	6.6	43.9	20.9	69.6
Case 35	Slot	Ceiling	15	17.5	63.5	21.3	70.4
Case 34	Radial	Ceiling	15	17.5	63.5	21.4	70.4
Case 36	Low Ind	Ceiling	15	17.5	63.5	21.5	70.6
Case 55	Low Ind	Ceiling	15	18.8	65.8	21.6	70.9
Case 97	Slot	Ceiling	15	17.5	63.5	21.6	71.0
Case 54	Slot	Ceiling	15	18.8	65.8	21.7	71.0
Case 53	Radial	Ceiling	15	18.8	65.8	21.7	71.0
Case 98	Low ind	Ceiling	15	17.5	63.5	21.7	71.1
Case 19	Radial	Ceiling	15	18.8	65.8	21.7	71.1
Case 96	Radial	Ceiling	15	17.5	63.5	21.8	71.2
Case 94	Slot	Ceiling	15	17.5	63.5	21.9	71.3
Case 67	Low Ind	Low	10	14.8	58.6	21.9	71.4
Case 75	Radial	Ceiling/Low 50/50	15	18.8	65.8	21.9	71.4
Case 60	Low Ind	High	15	18.8	65.8	21.9	71.4
Case 63	Low Ind	Low	10	14.8	58.6	21.9	71.4
Case 90	Radial	Ceiling	15	18.8	65.8	21,9	71.5
Case 95	Low Ind	Ceiling	15	17.5	63.5	21.9	71.5
Case 93	Radial	Ceiling	15	17.5	63.5	21.9	71.5
Case 92	Low Ind	Ceiling	15	18.8	65.8	22.0	71.6
Case 91	Slot	Ceiling	15	18.8	65.8	22.0	71.6
Case 43	Low Ind (rot 90°)	Ceiling	15	18.8	65.8	22.0	71.6
Case 46	Radial	Ceiling	15	18.8	65.8	22.0	71.6
Case 21	Low Ind	Ceiling	15	18.8	65.8	22.0	71.6
Case 59	Slot	Low	15	18.8	65.8	22.0	71.6
Basecase	Radial	Ceiling	15	18.8	65.8	22.1	71.7
Case 18	Low Ind	Ceiling	15	18.8	65.8	22.1	71.7
Case 45	Radial	Ceiling	15	18.8	65.8	22.1	71.7
Case 17	Slot	Ceiling	15	18.8	65.8	22.1	71.7
Case 20	Slot	Ceiling	15	18.8	65.8	22.1	71.7
Case 47	Radial	Ceiling	15	18.8	65.8	22. 1	71.7
Case 78	Low Ind	Ceiling	15	18.8	65.8	22.1	71.8
Case 56	Radial	High	15	18.8	65.8	22.1	71.8
Case 27	Low Ind	Ceiling	15	18.8	65.8	22.1	71.8
Case 48	Radial	Ceiling	15	18.8	65.8	22.1	71.8
Case 76	Radial	Ceiling	15	18.8	65.8	22.1	71.8
Case 26	Slot	Ceiling	15	18.8	65.8	22.1	71.8
Case 77	Slot	Ceiling	15	18.8	65.8	22.1	71.9
Case 44	Radial (rot 90°)	Ceiling	15	18.8	65.8	22.2	71.9
Case 04	Slot	Ceiling	15	18.8	65.8	22.2	71.9
Case 50	Radial	Ceiling	15	18.8	65.8	22.2	71.9
Case 49	Radial	Ceiling	15	18.8	65.8	22.2	71.9
Case 40	Radial	Ceiling x4	15	18.8	65.8	22.2	71.9
Case 42	Low Ind	Ceiling x4	15	18.8	65.8	22.2	71.9
Case 51	Radial	Ceiling	15	18.8	65.8	22.2	71.9
Case 25	Radial	Ceiling	15	18.8	65.8	22.2	72.0
Case 83	Slot	2 Door exhausts	15	17.5	63.5	22.2	72.0
Case 08	Low Ind	High	15	18.8	65,8	22.2	72.0
Case 41	Slot	Ceiling x4	15	18.8	65,8	22.2	72.0
Case 37	Radial	Ceiling/Low 50/50	15	18.8	65.8	22.3	72.1
Case 05	Slot	High	15	18.8	65.8	22.3	72.1
Case 82	Radial	2 Door exhausts	15	18.8	65.8	22.3	72.1
Case 86	I Slot	Low	15	17.5	63.5	22.3	72.1

Volume II - Section III - Rankings of Results of Whole Room Runs

Paee II - 41

Case Name	Supply Diffuser Type	Exhaust Location and Number	Supply ACH	Supply Temperature (°C)	Supply Temperature (°F)	Cage Temp (°C)	Cage Temp (°F)
Case 02	Radial	High	15	18.8	65.9	22.3	72.2
Case 85	Radial	Low	15	17.5	63.5	22.4	72.3
Case 38	Slot	Ceiling/Low 50/50	15	18.8	65.8	22.4	72.3
Case 39	Low Ind	Ceiling/Low 50/50	15	18.8	65.8	22.4	72.3
Case 65	Low Ind	Low	15	17.5	63.5	22.4	72.4
Case 07	Low Ind	Ceiling	15	18.8	65.8	22.5	72.4
Case 52	Radial	Ceiling	15	18.8	65.8	22.5	72.5
Case 22	Radial	Low	15	18.8	65.8	22.5	72.5
Case 84	Low Ind	2 Door exhausts	15	17.5	63.5	22.5	72.5
Case 72	Radial	High x4 / Low x2	15	18.8	65.8	22.5	72.6
Case 73	Slot	High x4 / Low x2	15	18.8	65.8	22.6	72.7
Case 74	Low Ind	High x4 / Low x2	15	18.8	65.8	22.6	72.7
Case 29	Low Ind	Low	5	II	51.8	22.6	72.8
Case 87	Radial	Low	15	19.2	66.6	22.7	72.8
Case 64	Low Ind	Low	20	189	66.0	22.7	72.8
Case 31	Radial	Low	15	17.5	63.5	22.7	72.8
Case 23	Slot	Low	15	18.8	65.8	22.7	72.9
Case 69	Radial	High x4 / Low x4	15	18.8	65.8	22.7	72.9
Case 11	Radial	Low	15	20.7	69.3	22.7	72.9
Case 68	Low Ind	Low	20	18.9	66.0	22.8	73.0
Case 13	Slot	Low	15	20.7	69.3	22.8	73.0
Case 14	Low Ind	Ceiling	15	20.7	69.3	22.8	73.0
Case 16	Radial	Ceiling	15	18.8	65.8	22.9	73.1
Case 70	Slot	High x4 / Low x4	15	18.8	65.8	22.9	73.2
Case 32	Slot	Low	15	17.5	63.5	22.9	73.2
Case 12	Slot	Ceiling	15	20.7	69.3	22.9	73.2
Case 71	Low Ind	High x4 / Low x4	15	18.8	65.8	23.0	73.3
Case 57	Radial	Low	15	18.8	65.8	23.0	73.4
Case 58	Slot	High	15	18.8	65.8	23.1	73.5
Case 79	Radial	2 Door exhausts	15	18.8	65.8	23.1	73.6
Case 61	Low Ind	Low	15	18.8	65.8	23.1	73.6
Case 33	Low Ind	Low	15	17.5	63.5	23.2	73.7
Case 24	Low Ind	Low	15	18.8	65.8	23.2	73.7
Case 80	Slot	2 Door exhausts	15	18.8	65.8	23.2	73.8
Case 10	Radial	Ceiling	15	20.7	69.3	23.2	73.8
Case 15	Low Ind	Low	15	20.7	69.3	23.2	73.8
Case 28	Low Ind	Low	10	16.8	62.2	23.3	74.0
Case 03	Radial	Low	15	18.8	65.8	23.4	74.0
Case 81	Low Ind	2 Door exhausts	15	18.8	65.8	23.4	74.1
Case 06	Slot	Low	15	18.8	65.8	23.4	74.2
Case 88	Slot	Low	15	19.2	66.6	23.5	74.4
Case 09	Low Ind	Low	15	18.8	65.8	23.6	74.5
Case 30	Low Ind	Low	20	19.8	67.6	23.6	74.6
Case 89	Low Ind	Low	15	19.2	66.6	23.7	74.6

Volume II -42

Ventilation Design Handbook on Animal Research Facilities Using Static Microisolators

Table 3.03 Ranking of Cases with respect to Room Relative Humidity

Case Name	Supply Diffuser Type	Exhaust Location and Number	Supply ACH	Supply Temperature (°C)	Supply Temperature (°F)	Room RH ( percent)
Case 89	Low Ind	Low	15	19.2	66.6	47.1
Case 88	Slot	Low	15	19,2	66.6	48.1
Case 30	Low Ind	Low	20	19.8	67.6	48.2
Case 09	Low Ind	Low	15	18.8	65.8	48.4
Case 87	Radial	Low	15	19.2	66.6	48.4
Case 81	Low Ind	2 Door exhausts	15	18.8	65.8	48.7
Case 06	Slot	Low	15	18.8	65.8	49.2
Case 28	Low Ind	Low	10	16.8	62.2	49.5
Case 03	Radial	Low	15	18.8	65.8	49.7
Case 24	Low Ind	Low	15	18.8	65.8	49.8
Case 80	Slot	2 Door exhausts	15	18.8	65.8	49.8
Case 61	Low Ind	Low	15	18.8	65.8	49.8
Case 79	Radial	2 Door exhausts	15	18.8	65.8	50.3
Case 32	Slot	Low	15	17.5	63.5	50.5
Case 58	Slot	High	15	18.8	65.8	50.6
Case 33	Low Ind	Low	15	17.5	63.5	50.7
Case 23	Slot	Low	15	18.8	65.8	50.7
Case 57	Radial	Low	15	18.8	65,8	50.7
Case 71	Low Ind	High x4 / Low x4	15	18.8	65.8	51.2
Case 70	Slot	High x4 / Low x4	15	18.8	65.8	51.4
Case 11	Radial	Low	15	20.7	69.3	52.0
Case 69	Radial	High x4 / Low x4	15	18.8	65.8	52.2
Case 31	Radial	Low	15	17.5	63.5	52.3
Case 15	Low Ind	Low	15	20.7	69.3	52.3
Case 84	Low Ind	2 Door exhausts	15	17.5	63.5	52,3
Case 22	Radial	Low	15	18.8	65.8	52.3
Case 12	Slot	Ceiling	15	20.7	69.3	52.3
Case 73	Slot	High x4 / Low x2	15	18.8	65.8	52.6
Case 83	Slot	2 Door exhausts	15	17.5	63,5	52.6
Case 68	Low Ind	Low	20	18.9	66.0	52.7
Case 14	Low Ind	Ceiling	15	20.7	69.3	52.7
Case 74	Low Ind	High x4 / Low x2	15	18.8	65.8	52.7
Case 75	Radial	Ceiling/Low 50/50	15	18.8	65.8	52.7
Case 82	Radial	2 Door exhausts	15	18.8	65.8	52.8
Case 72	Radial	High x4 / Low x2	15	18,8	65.8	52.8
Case 64	Low Ind	Low	20	18.9	66.0	52.9
Case 86	Slot	Low	15	17.5	63.5	53.1
Case 10	Radial	Ceiling	15	20.7	69.3	53.1
Case 29	Low Ind	Low	5	11	51.8	53.2
Case 13	Slot	Low	15	20.7	69.3	53.5
Case 38	Slot	Ceiling/Low 50/50	15	18.8	65.8	53.9
Case 39	Low Ind	Ceiling/Low 50/50	15	18.8	65.8	54.0
Case 37	Radial	Ceiling/Low 50/50	15	18.8	65.8	54.2
Case 02	Radial	High	15	18.8	65.8	54,3
Case 40	Radial	Ceiling x4	15	18.8	65.8	54.4
Case 65	Low Ind	Low	15	17,5	63.5	54.4
Case 48	Radial	Ceiling	15	18.8	65.8	54.5
Case 49	Radial	Ceiling	15	18.8	65.8	54.6
Case 05	Slot	High	15	18.8	65.8	54.6
Case 41	Slot	Ceiling x4	15	18.8	65.8	54.6
Case 85	Radial	Low	15	17.5	63.5	54.7
Case 20	Slot	Ceiling	15	18.8	65.8	54.8
Case 42	Low Ind	Ceiling x4	15	18.8	65.8	54.8
Case 51	Radial	Ceiling	15	18.8	65.8	54.8

Volume II - Section III - Rankings of Results of Whole Room Runs

Paee II - 43

Case Name	Supply DiffOser Type	Exhaust Location and Number	Supply ACH	Supply Temperature (°C)	Supply Temperature (°F)	Room RH ( percent)
Case 50	Radial	Ceiling	15	18.8	65.8	54.9
Case 91	Slot	Ceiling	15	18.8	65.8	55.0
Case 08	Low Ind	High	15	18.8	65.8	55.0
Case 26	Slot	Ceiling	15	18.8	65.8	55.0
Case 59	Slot	Low	15	18.8	65.8	55.0
Case 52	Radial	Ceiling	15	18.8	65.8	55.0
Case 92	Low Ind	Ceiling	15	18.8	65.8	55.0
Case 78	Low bd	Ceiling	15	18.8	65.8	55.1
Case 04	Slot	Ceiling	15	18.8	65.8	55.1
Case 77	Slot	Ceiling	15	18.8	65.8	55.2
Case 44	Radial (rot 90°)	Ceiling	15	18.8	65.8	55.2
Case 76	Radial	Ceiling	15	18.8	65.8	55.2
Case 21	Low bd	Ceiling	15	18.8	65.8	55.2
Case 90	Radial	Ceiling	15	18.8	65.8	55.4
Basecase	Radial	Ceiling	15	18.8	65.8	55.4
Case 56	Radial	High	15	18.8	65.8	55.5
Case 47	Radial	Ceiling	15	18.8	65.8	55.5
Case 27	Low bd	Ceiling	15	18.8	65.8	55.5
Case 25	Radial	Ceiling	15	18.8	65.8	55.6
Case 45	Radial	Ceiling	15	18.8	65.8	55.6
Case 60	Low bd	High	15	18.8	65.8	55.6
Case 43	Low bd (rot 90°)	Ceiling	15	18.8	65.8	55.8
Case 95	Low bd	Ceiling	15	17.5	63.5	55.9
Case 46	Radial	Ceiling	15	18.8	65.8	55.9
Case 19	Radial	Ceiling	15	18.8	65.8	55.9
Case 93	Radial	Ceiling	15	17.5	63.5	55.9
Case 17	Slot	Ceiling	15	18.8	65.8	56.0
Case 18	Low bd	Ceiling	15	18.8	65.8	56.0
Case 16	Radial	Ceiling	15	18.8	65.8	56.2
Case 94	Slot	Ceiling	15	17.5	63.5	56.3
Case 07	Low bd	Ceiling	15	18.8	65.8	56.7
Case 63	Low bd	Low	10	14.8	58.6	56.8
Case 98	Low ind	Ceiling	15	17.5	63.5	56.9
Case 96	Radial	Ceiling	15	17.5	63.5	57.0
Case 97	Slot	Ceiling	15	17.5	63.5	57.0
Case 67	Low bd	Low	10	14.8	58.6	57.2
Case 35	Slot	Ceiling	15	17.5	63.5	57.4
Case 55	Low bd	Ceiling	15	18.8	65.8	57.4
Case 54	Slot	Ceiling	15	18.8	65.8	57.4
Case 53	Radial	Ceiling	15	18.8	65.8	57.5
Case 34	Radial	Ceiling	15	17.5	63.5	57.5
Case 36	Low bd	Ceiling	15	17.5	63.5	57.7
Case 62	Low bd	Low	5	6.6	43.9	62.6
Case 66	Low bd	Low	5	6.6	43.9	64.4

Volume II -44

Ventilation Desi2n Handbook on Animal Research Facilities Using Static Microisolators

Table 3.04 Ranking of Cases with respect to Cage RH

Case Name	Supply Diffuser Type	Exhaust Location and Number	Supply ACH	Supply Temperature (°C)	Supply Temperature (“F)	Cage RH ( percent)
Case 62	Low Ind	Low	5	6.6	43.9	52.8
Case 66	Low Ind	Low	5	6.6	43.9	53.0
Case 84	Low Ind	2 Door exhausts	15	17.5	63.5	53.0
Case 29	Low Ind	Low	5	11	51.8	55.8
Case 87	Radial	Low	15	19.2	66.6	56.3
Case 64	Low Ind	Low	20	18.9	66.0	56.5
Case 23	Slot	Low	15	18.8	65.8	56.9
Case 69	Radial	High x4 / Low x4	15	18.8	65.8	57.1
Case 68	Low Ind	Low	20	18.9	66.0	57.5
Case 32	Slot	Low	15	17.5	63.5	57.5
Case 57	Radial	Low	15	18.8	65.8	58.3
Case 79	Radial	2 Door exhausts	15	18.8	65.8	58.6
Case 33	Low Ind	Low	15	17.5	63.5	58.7
Case 24	Low bd	Low	15	18.8	65.8	58.7
Case 80	Slot	2 Door exhausts	15	18.8	65.8	59.1
Case 28	Low Ind	Low	10	16.8	62.2	59.1
Case 03	Radial	Low	15	18.8	65.8	60.2
Case 81	Low Ind	2 Door exhausts	15	18.8	65.8	60.2
Case 06	Slot	Low	15	18.8	65.8	60.2
Case 88	Slot	Low	15	19.2	66.6	60.4
Case 09	Low Ind	Low	15	18.8	65.8	60.6
Case 30	Low Ind	Low	20	19.8	67.6	60.9
Case 89	Low Ind	Low	15	19.2	66.6	60.9
Case 22	Radial	Low	15	18.8	65.8	61.1
Case 31	Radial	Low	15	17.5	63.5	61.4
Case 58	Slot	High	15	18.8	65.8	61.4
Case 61	Low Ind	Low	15	18.8	65.8	61.6
Case 71	Low Ind	High x4 / Low x4	15	18.8	65.8	61.6
Case 10	Radial	Ceiling	15	20.7	69.3	61.7
Case 15	Low Ind	Low	15	20.7	69.3	61.7
Case 70	Slot	High x4 / Low x4	15	18.8	65.8	61.8
Case 82	Radial	2 Door exhausts	15	18.8	65.8	61.9
Case 72	Radial	High x4 / Low x2	15	18.8	65.8	61.9
Case 86	Slot	Low	15	17.5	63.5	62.0
Case 02	Radial	High	15	18.8	65.8	62.0
Case 41	Slot	Ceiling x4	15	18.8	65.8	62.3
Case 74	Low Ind	High x4 / Low x2	15	18.8	65.8	62.3
Case 37	Radial	Ceiling/Low 50/50	15	18.8	65.8	62.4
Case 38	Slot	Ceiling/Low 50/50	15	18.8	65.8	62.4
Case 05	Slot	High	15	18.8	65.8	62.5
Case 20	Slot	Ceiling	15	18.8	65.8	62.6
Case 65	Low Ind	Low	15	17.5	63.5	62.7
Case 04	Slot	Ceiling	15	18.8	65.8	62.7
Case 42	Low Ind	Ceiling x4	15	18.8	65.8	62.8
Case 92	Low Ind	Ceiling	15	18.8	65.8	62.8
Case 85	Radial	Low	15	17.5	63.5	62.8
Case 39	Low Ind	Ceiling/Low 50/50	15	18.8	65.8	62.9
Case 73	Slot	High x4 / Low x2	15	18.8	65.8	62.9
Case 90	Radial	Ceiling	15	18.8	65.8	63.3
Case 91	Slot	Ceiling	15	18.8	65.8	63.4
Case 11	Radial	Low	15	20.7	69.3	63.6
Case 12	Slot	Ceiling	15	20.7	69.3	63.6
Case 50	Radial	Ceiling	15	18.8	65.8	63.7
Case 18	Low Ind	Ceiling	15	18.8	65.8	63.8

Volume II - Section III - Rankings of Results of Whole Room Runs

Page II - 45

Case Name	Supply Diffuser Type	Exhaust Location and Number	Supply ACH	Supply Temperature (°C)	Supply Temperature (°F)	Cage RH ( percent)
Case 08	Low Ind	High	15	18.8	65.8	63.8
Case 17	Slot	Ceiling	15	18.8	65.8	63.9
Case 16	Radial	Ceiling	15	18.8	65.8	64.0
Case 76	Radial	Ceiling	15	18.8	65.8	64.1
Case 21	Low Ind	Ceiling	15	18.8	65.8	64.1
Case 95	Low Ind	Ceiling	15	17.5	63.5	64.1
Case 77	Slot	Ceiling	15	18.8	65.8	64.2
Case 14	Low Ind	Ceiling	15	20.7	69.3	64.2
Case 97	Slot	Ceiling	15	17.5	63.5	64.2
Case 94	Slot	Ceiling	15	17.5	63.5	64.4
Case 56	Radial	High	15	18.8	65.8	64.4
Case 43	Low Ind (rot 90°)	Ceiling	15	18.8	65.8	64.6
Case 19	Radial	Ceiling	15	18.8	65.8	64.6
Case 78	Low Ind	Ceiling	15	18.8	65.8	64.7
Case 52	Radial	Ceiling	■ 15	18.8	65.8	64.8
Case 40	Radial	Ceiling x4	15	18.8	65.8	64.8
Case 45	Radial	Ceiling	15	18.8	65.8	64.9
Case 75	Radial	Ceiling/Low 50/50	15	18.8	65.8	64.9
Case 63	Low Ind	Low	10	14.8	58.6	64.9
Case 93	Radial	Ceiling	15	17.5	63.5	65.0
Case 47	Radial	Ceiling	15	18.8	65.8	65.1
Case 49	Radial	Ceiling	15	18.8	65.8	65.2
Case 46	Radial	Ceiling	15	18.8	65.8	65.2
Case 51	Radial	Ceiling	15	18.8	65.8	65.3
Case 35	Slot	Ceiling	15	17.5	63.5	65.5
Case 67	Low Ind	Low	10	14.8	58.6	65.8
Case 98	Low ind	Ceiling	15	17.5	63.5	65.8
Case 96	Radial	Ceiling	15	17.5	63.5	65.8
Case 54	Slot	Ceiling	15	18.8	65.8	66.0
Case 44	Radial (rot 90°)	Ceiling	15	18.8	65.8	66.0
Case 48	Radial	Ceiling	15	18.8	65.8	66.1
Case 07	Low Ind	Ceiling	15	18.8	65.8	66.4
Case 13	Slot	Low	15	20.7	69.3	66.5
Case 36	Low Ind	Ceiling	15	17.5	63.5	66.5
Base case	Radial	Ceiling	15	18.8	65.8	66.8
Case 83	Slot	2 Door exhausts	15	17.5	63.5	66.8
Case 59	Slot	Low	15	18.8	65.8	66.9
Case 34	Radial	Ceiling	15	17.5	63.5	67.2
Case 55	Low Ind	Ceiling	15	18.8	65.8	67.8
Case 53	Radial	Ceiling	15	18.8	65.8	68.8
Case 60	Low Ind	High	15	18.8	65.8	69.1
Case 25	Radial	Ceiling	15	18.8	65.8	73.0
Case 27	Low Ind	Ceiling	15	18.8	65.8	74.8
Case 26	Slot	Ceiling	15	18.8	65.8	81.2

Volume II -46

Ventilation Design Handbook on Animal Research Facilities Using Static Microisolators

Table 3.05 Ranking of Cases with respect to Room CO2

Case Name	Supply Diffuser Type	Exhaust Location and Number	Supply ACH	Supply Temperature (°C)	Supply Temperature (°F)	Room CO2 (ppm)
Case 59	Slot	Low	15	18.8	65.8	15
Case 12	Slot	Ceiling	15	20.7	69.3	17
Case 40	Radial	Ceiling x4	15	18.8	65.8	21
Case 70	Slot	High x4 / Low x4	15	18.8	65.8	24
Case 10	Radial	Ceiling	15	20.7	69.3	29
Case 26	Slot	Ceiling	15	18.8	65.8	35
Basecase	Radial	Ceiling	15	18.8	65.8	36
Case 13	Slot	Low	15	20.7	69.3	37
Case 16	Radial	Ceiling	15	18.8	65.8	37
Case 73	Slot	High x4 / Low x2	15	18.8	65.8	37
Case 47	Radial	Ceiling	15	18.8	65.8	40
Case 92	Low Ind	Ceiling	15	18.8	65.8	41
Case 46	Radial	Ceiling	15	18.8	65.8	43
Case 87	Radial	Low	15	19.2	66.6	45
Case 11	Radial	Low	15	20.7	69.3	48
Case 74	Low Ind	High x4 / Low x2	15	18.8	65.8	48
Case 75	Radial	Ceiling/Low 50/50	15	18.8	65.8	48
Case 88	Slot	Low	15	19.2	66.6	48
Case 15	Low Ind	Low	15	20.7	69.3	48
Case 72	Radial	High x4 / Low x2	15	18.8	65.8	48
Case 05	Slot	High	15	18.8	65.8	49
Case 77	Slot	Ceiling	15	18.8	65.8	50
Case 41	Slot	Ceiling x4	15	18.8	65.8	51
Case 69	Radial	High x4 / Low x4	15	18.8	65.8	51
Case 71	Low Ind	High x4 / Low x4	15	18.8	65.8	51
Case 49	Radial	Ceiling	15	18.8	65.8	52
Case 14	Low Ind	Ceiling	15	20.7	69.3	52
Case 45	Radial	Ceiling	15	18.8	65.8	52
Case 50	Radial	Ceiling	15	18.8	65.8	55
Case 76	Radial	Ceiling	15	18.8	65.8	55
Case 78	Low Ind	Ceiling	15	18.8	65.8	55
Case 60	Low Ind	High	15	18.8	65.8	57
Case 07	Low Ind	Ceiling	15	18.8	65.8	57
Case 55	Low Ind	Ceiling	15	18.8	65.8	57
Case 91	Slot	Ceiling	15	18.8	65.8	57
Case 57	Radial	Low	15	18.8	65.8	57
Case 53	Radial	Ceiling	15	18.8	65.8	58
Case 37	Radial	Ceiling/Low 50/50	15	18.8	65.8	59
Case 19	Radial	Ceiling	15	18.8	65.8	59
Case 54	Slot	Ceiling	15	18.8	65.8	60
Case 25	Radial	Ceiling	15	18.8	65.8	60
Case 22	Radial	Low	15	18.8	65.8	61
Case 27	Low Ind	Ceiling	15	18.8	65.8	61
Case 21	Low Ind	Ceiling	15	18.8	65.8	62
Case 04	Slot	Ceiling	15	18.8	65.8	62
Case 89	Low Ind	Low	15	19.2	66.6	62
Case 38	Slot	Ceiling/Low 50/50	15	18.8	65.8	62
Case 23	Slot	Low	15	18.8	65.8	63
Case 42	Low Ind	Ceiling x4	15	18.8	65.8	63
Case 30	Low Ind	Low	20	19.8	67.6	64
Case 08	Low Ind	High	15	18.8	65.8	64
Case 03	Radial	Low	15	18.8	65.8	64
Case 48	Radial	Ceiling	15	18.8	65.8	65
Case 02	Radial	High	15	18.8	65.8	65

Volume II - Section III - Rankings of Results of Whole Room Runs

Page II - 47

Case Name	Supply Diffuser Type	Exhaust Location and Number	Supply ACH	Supply Temperature (°C)	Supply Temperature (°F)	Room CO2 (ppm)
Case 52	Radial	Ceiling	15	18.8	65.8	65
Case 43	Low Ind (rot 90°)	Ceiling	15	18.8	65.8	65
Case 51	Radial	Ceiling	15	18.8	65.8	67
Case 58	Slot	High	15	18.8	65.8	68
Case 61	Low Ind	Low	15	18.8	65.8	69
Case 56	Radial	High	15	18.8	65.8	69
Case 95	Low Ind	Ceiling	15	17.5	63.5	69
Case 39	Low Ind	Ceiling/Low 50/50	15	18.8	65.8	71
Case 06	Slot	Low	15	18.8	65.8	72
Case 90	Radial	Ceiling	15	18.8	65.8	72
Case 44	Radial (rot 90°)	Ceiling	15	18.8	65.8	74
Case 20	Slot	Ceiling	15	18.8	65.8	75
Case 17	Slot	Ceiling	15	18.8	65.8	78
Case 18	Low Ind	Ceiling	15	18.8	65.8	79
Case 79	Radial	2 Door exhausts	15	18.8	65.8	81
Case 24	Low Ind	Low	15	18.8	65.8	81
Case 98	Low ind	Ceiling	15	17.5	63.5	83
Case 93	Radial	Ceiling	15	17.5	63.5	86
Case 09	Low Ind	Low	15	18.8	65.8	88
Case 81	Low Ind	2 Door exhausts	15	18.8	65.8	89
Case 80	Slot	2 Door exhausts	15	18.8	65.8	90
Case 68	Low Ind	Low	20	18.9	66.0	93
Case 64	Low Ind	Low	20	18.9	66.0	93
Case 96	Radial	Ceiling	15	17.5	63.5	100
Case 31	Radial	Low	15	17.5	63.5	107
Case 97	Slot	Ceiling	15	17.5	63,5	1 1 1
Case 28	Low Ind	Low	10	16.8	62.2	1 13
Case 82	Radial	2 Door exhausts	15	18.8	65.8	1 16
Case 94	Slot	Ceiling	15	17.5	63.5	1 17
Case 35	Slot	Ceiling	15	17.5	63.5	1 22
Case 86	Slot	Low	15	17.5	63,5	124
Case 32	Slot	Low	15	17.5	63.5	125
Case 85	Radial	Low	15	17.5	63.5	132
Case 65	Low Ind	Low	15	17.5	63.5	133
Case 36	Low Ind	Ceiling	15	17.5	63.5	134
Case 34	Radial	Ceiling	15	17.5	63.5	136
Case 29	Low Ind	Low	5	11	51.8	140
Case 33	Low Ind	Low	15	17.5	63.5	143
Case 84	Low Ind	2 Door exhausts	15	17.5	63.5	152
Case 83	Slot	2 Door exhausts	15	17.5	63.5	167
Case 67	Low Ind	Low	10	14.8	58.6	201
Case 63	Low Ind	Low	10	14.8	58.6	215
Case 66	Low Ind	Low	5	6.6	43.9	318
Case 62	Low Ind	Low	5	6.6	43.9	342

Volume II -48

Ventilation Design Handbook on Animal Research Facilities Using Static Microisolators

Table 3.06 Ranking of Cases with respect to Cage CO2

Case Name	Supply Diffuser Type	Exhaust Location and Number	Supply ACH	Supply Temperature (°C)	Supply Temperature (°F)	Cage COj (ppm)
Case 87	Radial	Low	15	19.2	66.6	1391
Case 88	Slot	Low	15	19.2	66.6	1550
Case 03	Radial	Low	15	18.8	65.8	1568
Case 92	Low Ind	Ceiling	15	18.8	65.8	1631
Case 89	Low Ind	Low	15	19.2	66.6	1636
Case 79	Radial	2 Door exhausts	15	18.8	65.8	1647
Case 23	Slot	Low	15	18.8	65.8	1652
Case 06	Slot	Low	15	18.8	65.8	1652
Case 20	Slot	Ceiling	15	18.8	65.8	1656
Case 52	Radial	Ceiling	15	18.8	65.8	1665
Case 22	Radial	Low	15	18.8	65.8	1665
Case 82	Radial	2 Door exhausts	• 15	18.8	65.8	1667
Case 30	Low Ind	Low	20	19.8	67.6	1667
Case 90	Radial	Ceiling	15	18.8	65.8	1673
Case 41	Slot	Ceiling x4	15	18.8	65.8	1690
Case 02	Radial	High	15	18.8	65,8	1703
Case 80	Slot	2 Door exhausts	15	18.8	65.8	1707
Case 04	Slot	Ceiling	15	18.8	65.8	1709
Case 91	Slot	Ceiling	15	18.8	65.8	1709
Case 37	Radial	Ceiling/Low 50/50	15	18.8	65,8	1715
Case 97	Slot	Ceiling	15	17.5	63.5	1725
Case 09	Low Ind	Low	15	18.8	65.8	1725
Case 19	Radial	Ceiling	15	18.8	65,8	1725
Case 42	Low Ind	Ceiling x4	15	18.8	65.8	1728
Case 81	Low Ind	2 Door exhausts	15	18.8	65.8	1741
Case 05	Slot	High	15	18.8	65.8	1742
Case 32	Slot	Low	15	17.5	63.5	1747
Case 28	Low Ind	Low	10	16.8	62.2	1749
Case 69	Radial	High x4 / Low x4	15	18.8	65.8	1750
Case 83	Slot	2 Door exhausts	15	17.5	63.5	1756
Case 94	Slot	Ceiling	15	17.5	63.5	1757
Case 35	Slot	Ceiling	15	17,5	63.5	1761
Case 29	Low Ind	Low	5	1 1	51.8	1764
Case 38	Slot	Ceiling/Low 50/50	15	18.8	65.8	1776
Case 72	Radial	High x4 / Low x2	15	18.8	65.8	1790
Case 18	Low Ind	Ceiling	15	18.8	65.8	1791
Case 64	Low Ind	Low	20	18.9	66.0	1808
Case 21	Low Ind	Ceiling	15	18.8	65.8	1808
Case 17	Slot	Ceiling	15	18.8	65.8	1815
Case 95	Low Ind	Ceiling	15	17.5	63.5	1822
Case 93	Radial	Ceiling	15	17.5	63.5	1822
Case 68	Low Ind	Low	20	18.9	66.0	1838
Case 75	Radial	Ceiling/Low 50/50	15	18.8	65.8	1839
Case 50	Radial	Ceiling	15	18.8	65.8	1840
Case 39	Low Ind	Ceiling/Low 50/50	15	18.8	65.8	1848
Case 84	Low Ind	2 Door exhausts	15	17.5	63.5	1853
Case 57	Radial	Low	15	18.8	65.8	1855
Case 43	Low Ind (rot 90°)	Ceiling	15	18.8	65.8	1855
Case 76	Radial	Ceiling	15	18.8	65.8	1857
Case 54	Slot	Ceiling	15	18.8	65.8	1869
Case 08	Low Ind	High	15	18.8	65.8	1871
Case 36	Low Ind	Ceiling	15	17.5	63.5	1878
Case 77	Slot	Ceiling	15	18.8	65.8	1881
Case 24	Low Ind	Low	15	18.8	65.8	1883

Volume II - Section III - Rankings of Results of Whole Room Runs

Page II - 49

Case Name	Supply Diffuser Type	Exhaust Location and Number	Supply ACH	Supply Temperature (°C)	Supply Temperature (°F)	Cage CO2 (ppm)
Case 74	Low Ind	High x4 / Low x2	15	18.8	65.8	1884
Case 56	Radial	High	15	18.8	65.8	1886
Case 34	Radial	Ceiling	15	17.5	63.5	1896
Case 86	Slot	Low	15	17.5	63.5	1917
Case 78	Low Ind	Ceiling	15	18.8	65,8	1919
Case 45	Radial	Ceiling	15	18.8	65.8	1929
Case 85	Radial	Low	15	17.5	63.5	1930
Case 31	Radial	Low	15	17.5	63.5	1938
Case 46	Radial	Ceiling	15	18.8	65.8	1943
Case 98	Low ind	Ceiling	15	17.5	63.5	1955
Case 70	Slot	High x4 / Low x4	15	18.8	65.8	1955
Case 96	Radial	Ceiling	15	17.5	63.5	1957
Case 47	Radial	Ceiling	15	18.8	65.8	1961
Case 73	Slot	High x4 / Low x2	15	18.8	65.8	1966
Case 71	Low Ind	High x4 / Low x4	15	18.8	65.8	1968
Case 33	Low Ind	Low	15	17.5	63.5	1974
Case 40	Radial	Ceiling x4	15	18.8	65.8	1975
Case 58	Slot	High	15	18.8	65.8	1990
Case 65	Low Ind	Low	15	17.5	63.5	2002
Case 49	Radial	Ceiling	15	18.8	65.8	2025
Case 55	Low Ind	Ceiling	15	18.8	65.8	2039
Case 61	Low Ind	Low	15	18.8	65.8	2042
Case 51	Radial	Ceiling	15	18.8	65.8	2045
Case 63	Low Ind	Low	10	14.8	58.6	2081
Case 48	Radial	Ceiling	15	18.8	65.8	2099
Case 11	Radial	Low	15	20.7	69.3	2118
Case 44	Radial (rot 90°)	Ceiling	15	18.8	65.8	2118
Case 10	Radial	Ceiling	15	20.7	69.3	2119
Case 15	Low Ind	Low	15	20.7	69.3	2119
Case 67	Low Ind	Low	10	14.8	58.6	2144
Basecase	Radial	Ceiling	15	18.8	65.8	2158
Case 59	Slot	Low	15	18.8	65.8	2161
Case 12	Slot	Ceiling	15	20.7	69.3	2208
Case 53	Radial	Ceiling	15	18.8	65.8	2212
Case 16	Radial	Ceiling	15	18.8	65.8	2213
Case 14	Low Ind	Ceiling	15	20.7	69.3	2215
Case 66	Low Ind	Low	5	6.6	43.9	2318
Case 07	Low Ind	Ceiling	15	18.8	65.8	2321
Case 62	Low Ind	Low	5	■ 6.6	43.9	2325
Case 60	Low Ind	High	15	18.8	65.8	2354
Case 13	Slot	Low	15	20.7	69.3	2491
Case 25	Radial	Ceiling	15	18.8	65.8	3008
Case 27	Low Ind	Ceiling	15	18.8	65.8	3171
Case 26	Slot	Ceiling	15	18.8	65.8	3975

Volume II -50

Ventilation Desien Handbook on Animal Research Facilities Using Static Microisolators

Table 3.07 Ranking of Cases with respect to Room NH3

Case Name	Supply Diffuser Type	Exhaust Location and Number	Supply ACH	Supply Temperature (°C)	Supply Temperature (°F)	Room NH.1 (day 4) (ppm)
Case 59	Slot	Low	15	18.8	65.8	0.04
Case 12	Slot	Ceiling	15	20.7	69.3	0.04
Case 70	Slot	High x4 / Low x4	15	18.8	65.8	0.06
Case 40	Radial	Ceiling x4	15	18.8	65.8	0.06
Case 10	Radial	Ceiling	15	20.7	69.3	0.07
Case 73	Slot	High x4 / Low x2	15	18.8	65.8	0.09
Case 16	Radial	Ceiling	15	18.8	65.8	0.10
Case 87	Radial	Low	15	19.2	66.6	0.10
Case 92	Lx)w Ind	Ceiling	15	18.8	65.8	0.10
Basecase	Radial	Ceiling	15	18.8	65.8	0.11
Case 13	Slot	Low	15	20.7	69.3	0.11
Case 88	Slot	Low	15	19.2	66.6	0.1 1
Case 47	Radial	Ceiling	15	18.8	65.8	0.1 1
Case 15	Low Ind	Low	15	20.7	69.3	0.1 1
Case 72	Radial	High x4 / Low x2	15	18.8	65.8	0.11
Case 74	Low Ind	High x4 / Low x2	15	18.8	65.8	0.11
Case 69	Radial	High x4 / Low x4	15	18.8	65.8	0.12
Case 46	Radial	Ceiling	15	18.8	65.8	0.12
Case 71	Low Ind	High x4 / Low x4	15	18.8	65.8	0.12
Case 05	Slot	High	15	18.8	65.8	0.12
Case 41	Slot	Ceiling x4	15	18.8	65.8	0.12
Case 11	Radial	Low	15	20.7	69.3	0.12
Case 57	Radial	Low	15	18.8	65.8	0.13
Case 75	Radial	Ceiling/Low 50/50	15	18.8	65.8	0.13
Case 77	Slot	Ceiling	15	18.8	65.8	0.13
Case 14	Low Ind	Ceiling	15	20.7	69.3	0.14
Case 22	Radial	Low	15	18.8	65.8	0.14
Case 89	Low Ind	Low	15	19.2	66.6	0.14
Case 50	Radial	Ceiling	15	18.8	65.8	0.14
Case 37	Radial	Ceiling/Low 50/50	15	18,8	65.8	0.14
Case 23	Slot	Low	15	18.8	65.8	0.14
Case 45	Radial	Ceiling	15	18,8	65.8	0.14
Case 49	Radial	Ceiling	15	18.8	65.8	0.14
Case 30	Low Ind	Low	20	19.8	67.6	0.14
Case 03	Radial	Low	15	18.8	65.8	0.14
Case 76	Radial	Ceiling	15	18.8	65.8	0.14
Case 91	Slot	Ceiling	15	18.8	65.8	0.14
Case 78	Low Ind	Ceiling	15	18.8	65.8	0.15
Case 38	Slot	Ceiling/Low 50/50	15	18.8	65.8	0.15
Case 04	Slot	Ceiling	15	18.8	65.8	0.15
Case 02	Radial	High	15	18.8	65.8	0.15
Case 42	Low Ind	Ceiling x4	15	18.8	65.8	0.16
Case 58	Slot	High	15	18.8	65.8	0.16
Case 19	Radial	Ceiling	15	18.8	65.8	0.16
Case 61	Low Ind	Low	15	18.8	65.8	0.16
Case 26	Slot	Ceiling	15	18.8	65.8	0.16
Case 06	Slot	Low	15	18.8	65.8	0.16
Case 21	Low Ind	Ceiling	15	18.8	65.8	0.16
Case 07	Low Ind	Ceiling	15	18.8	65.8	0.16
Case 08	Low Ind	High	15	18.8	65.8	0.16
Case 54	Slot	Ceiling	15	18.8	65.8	0.17
Case 43	Low Ind (rot 90°)	Ceiling	15	18.8	65.8	0.17
Case 55	Low Ind	Ceiling	15	18.8	65.8	0.17
Case 52	Radial	Ceiling	15	18.8	65.8	0.18

Volume II - Section III - Rankings of Results of Whole Room Runs

Paee 11-51

Case Name	Supply Diffuser Type	Exhaust Location and Number	Supply ACH	Supply Temperature (°C)	Supply Temperature (°F)	Room NHj (day 4) (ppm)
Case 39	Low Ind	Ceiling/Low 50/50	15	18.8	65.8	0.18
Case 90	Radial	Ceiling	15	18.8	65.8	0.18
Case 60	Low Ind	High	15	18.8	65.8	0.18
Case 95	Low Ind	Ceiling	15	17.5	63.5	0.18
Case 20	Slot	Ceiling	15	18.8	65.8	0.18
Case 79	Radial	2 Door exhausts	15	18.8	65.8	0.18
Case 24	Low Ind	Low	15	18.8	65.8	0.18
Case 56	Radial	High	15	18.8	65.8	0.18
Case 48	Radial	Ceiling	15	18.8	65.8	0.18
Case 51	Radial	Ceiling	15	18.8	65.8	0.18
Case 53	Radial	Ceiling	15	18.8	65.8	0.18
Case 09	Low Ind	Low	15	18.8	65.8	0.20
Case 81	Low Ind	2 Door exhausts	15	18.8	65.8	0.20
Case 17	Slot	Ceiling	15	18.8	65.8	0.20
Case 80	Slot	2 Door exhausts	15	18.8	65.8	0.20
Case 18	Low Ind	Ceiling	15	18.8	65.8	0.20
Case 68	Low Ind	Low	20	18.9	66.0	0.21
Case 64	Low Ind	Low	20	18.9	66.0	0.21
Case 44	Radial (rot 90°)	Ceiling	15	18.8	65.8	0.21
Case 25	Radial	Ceiling	15	18.8	65.8	0.22
Case 98	Low ind	Ceiling	15	17.5	63.5	0.23
Case 93	Radial	Ceiling	15	17.5	63.5	0.24
Case 27	Low Ind	Ceiling	15	18.8	65.8	0.24
Case 31	Radial	Low	15	17.5	63.5	0.24
Case 28	Low Ind	Low	10	16.8	62.2	0.25
Case 82	Radial	2 Door exhausts	15	18.8	65.8	0.27
Case 32	Slot	Low	15	17.5	63.5	0.28
Case 96	Radial	Ceiling	15	17.5	63.5	0.28
Case 97	Slot	Ceiling	15	17.5	63.5	0.29
Case 86	Slot	Low	15	17.5	63.5	0.29
Case 94	Slot	Ceiling	15	17.5	63.5	0.31
Case 29	Low Ind	Low	5	1 1	51.8	0.32
Case 33	Low Ind	Low	15	17.5	63.5	0.32
Case 85	Radial	Low	15	17.5	63.5	0.33
Case 65	Low Ind	Low	15	17.5	63.5	0.33
Case 35	Slot	Ceiling	15	17.5	63.5	0.34
Case 84	Low Ind	2 Door exhausts	15	17.5	63.5	0.34
Case 36	Low Ind	Ceiling	15	17.5	63.5	0.39
Case 34	Radial	Ceiling	15	17.5	63.5	0.41
Case 83	Slot	2 Door exhausts	15	17.5	63.5	0.49
Case 67	Low Ind	Low	10	14.8	58.6	0.57
Case 63	Low Ind	Low	10	14.8	58.6	0.58
Case 66	Low Ind	Low	5	6.6	43.9	0.72
Case 62	Low Ind	Low	5	6.6	43.9	0.77

Volume II -52

Ventilation Design Handbook on Animal Research Facilities Using Static Microisolators

Table 3.08 Ranking of Cases with respect to Cage NH3 (Day 4)

Case Name	Supply Diffuser Type	Exhaust Location and Number	Supply ACH	Supply Temperature (°C)	Supply Temperature (°F)	Cage NHj (day 4) (ppm)
Case 87	Radial	Low	15	19.2	66.6	3.13
Case 88	Slot	Low	15	19.2	66.6	3.49
Case 03	Radial	Low	15	18.8	65.8	3.53
Case 89	Low Ind	Low	15	19.2	66.6	3.68
Case 79	Radial	2 Door exhausts	15	18.8	65.8	3.70
Case 23	Slot	Low	15	18.8	65.8	3,71
Case 06	Slot	Low	15	18.8	65.8	3.72
Case 30	Low Ind	Low	20	19.8	67.6	3.75
Case 22	Radial	Low	15	18.8	65.8	3.77
Case 80	Slot	2 Door exhausts	15	18.8	65,8	3.84
Case 09	Low Ind	Low	15	18.8	65.8	3.88
Case 81	Low Ind	2 Door exhausts	15	18.8	65.8	3.92
Case 82	Radial	2 Door exhausts	15	18.8	65.8	3.92
Case 32	Slot	Low	15	17.5	63.5	3.93
Case 28	Low Ind	Low	10	16.8	62.2	3.93
Case 69	Radial	High x4 / Low x4	15	18.8	65.8	3.94
Case 29	Low Ind	Low	5	1 1	51.8	3.97
Case 92	Low Ind	Ceiling	15	18.8	65.8	4.02
Case 02	Radial	High	15	18.8	65.8	4.03
Case 20	Slot	Ceiling	15	18,8	65.8	4.04
Case 41	Slot	Ceiling x4	15	18.8	65.8	4.06
Case 64	Low Ind	Low	20	18.9	66.0	4.07
Case 37	Radial	Ceiling/Low 50/50	15	18.8	65.8	4.13
Case 68	Low Ind	Low	20	18.9	66.0	4.13
Case 84	Low Ind	2 Door exhausts	15	17,5	63.5	4.17
Case 57	Radial	Low	15	18.8	65.8	4.17
Case 04	Slot	Ceiling	15	18.8	65.8	4.18
Case 72	Radial	High x4 / Low x2	15	18.8	65.8	4.22
Case 90	Radial	Ceiling	15	18.8	65.8	4.22
Case 05	Slot	High	15	18.8	65.8	4.23
Case 24	Low Ind	Low	15	18.8	65,8	4.24
Case 42	Low Ind	Ceiling x4	15	18.8	65,8	4.25
Case 38	Slot	Ceiling/Low 50/50	15	18.8	65.8	4.28
Case 91	Slot	Ceiling	15	18.8	65.8	4.33
Case 33	Low Ind	Low	15	17,5	63.5	4,44
Case 31	Radial	Low	15	17.5	63.5	4.45
Case 52	Radial	Ceiling	15	18.8	65.8	4.49
Case 74	Low Ind	High x4 / Low x2	15	18.8	65.8	4.53
Case 97	Slot	Ceiling	15	17.5	63.5	4.54
Case 86	Slot	Low	15	17.5	63.5	4.54
Case 71	Low Ind	High x4 / Low x4	15	18.8	65,8	4.56
Case 58	Slot	High	15	18.8	65.8	4.58
Case 39	Low Ind	Ceiling/Low 50/50	15	18.8	65.8	4.58
Case 70	Slot	High x4 / Low x4	15	18.8	65.8	4.58
Case 19	Radial	Ceiling	15	18.8	65.8	4.61
Case 18	Low Ind	Ceiling	15	18.8	65.8	4.62
Case 94	Slot	Ceiling	15	17.5	63,5	4.66
Case 17	Slot	Ceiling	15	18.8	65.8	4.71
Case 61	Low Ind	Low	15	18.8	65.8	4.73
Case 21	Low Ind	Ceiling	15	18.8	65.8	4.73
Case 50	Radial	Ceiling	15	18.8	65.8	4.73
Case 85	Radial	Low	15	17.5	63.5	4.76
Case 95	Low Ind	Ceiling	15	17.5	63.5	4.77

Volume II - Section III - Rankings of Results of Whole Room Runs

Page II - 53

Case Name	Supply Diffuser Type	Exhaust Location and Number	Supply ACH	Supply Temperature (°C)	Supply Temperature (°F)	Cage NHj (day 4) (ppm)
Case 08	Lx)w Ind	High	15	18.8	65.8	4.83
Case 76	Radial	Ceiling	15	18.8	65.8	4.85
Case 73	Slot	High x4 / Low x2	15	18.8	65.8	4.87
Case 65	Low Ind	Low	15	17.5	63.5	4.90
Case 35	Slot	Ceiling	15	17.5	63.5	4.90
Case 77	Slot	Ceiling	15	18.8	65.8	4.94
Case 10	Radial	Ceiling	15	20.7	69.3	4.94
Case 15	Low Ind	Low	15	20.7	69.3	4.94
Case 43	Low Ind (rot 90°)	Ceiling	15	18.8	65.8	4.96
Case 93	Radial	Ceiling	15	17.5	63.5	4.97
Case 75	Radial	Ceiling/Low 50/50	15	18.8	65.8	4.98
Case 56	Radial	High	15	18.8	65.8	5.01
Case 83	Slot	2 Door exhausts	15	17.5	63.5	5.15
Case 78	Low Ind	Ceiling	15	18.8	65.8	5.16
Case 66	Low Ind	Low	5	6 6	43.9	5.21
Case 45	Radial	Ceiling	15	18.8	65.8	5.22
Case 62	Low Ind	Low	5	6.6	43.9	5.23
Case 54	Slot	Ceiling	15	18.8	65.8	5.32
Case 40	Radial	Ceiling x4	15	18.8	65.8	5.33
Case 46	Radial	Ceiling	15	18.8	65.8	5.35
Case 47	Radial	Ceiling	15	18.8	65.8	5.36
Case 11	Radial	Low	15	20.7	69.3	5.42
Case 36	Low Ind	Ceiling	15	17.5	63.5	5.44
Case 98	Low ind	Ceiling	15	17.5	63.5	5.50
Case 96	Radial	Ceiling	15	17.5	63.5	5.51
Case 49	Radial	Ceiling	15	18.8	65.8	5.56
Case 51	Radial	Ceiling	15	18.8	65.8	5.64
Case 63	Low Ind	Low	10	14.8	58.6	5.64
Case 12	Slot	Ceiling	15	20.7	69.3	5.66
Case 34	Radial	Ceiling	15	17.5	63.5	5.66
Case 16	Radial	Ceiling	15	18.8	65.8	5.76
Case 14	Low Ind	Ceiling	15	20.7	69.3	5.82
Case 48	Radial	Ceiling	15	18.8	65.8	5.98
Case 44	Radial (rot 90°)	Ceiling	15	18.8	65.8	6.03
Case 67	Low Ind	Low	10	14.8	58.6	6.03
Case 55	Low Ind	Ceiling	15	18.8	65.8	6.22
Basecase	Radial	Ceiling	15	18.8	65.8	6.33
Case 59	Slot	Low	15	18.8	65.8	6.38
Case 07	Low Ind	Ceiling	15	18.8	65.8	6.71
Case 53	Radial	Ceiling	15	18.8	65.8	7.02
Case 13	Slot	Low	15	20.7	69.3	7.21
Case 60	Low Ind	High	15	18.8	65.8	7.56
Case 25	Radial	Ceiling	15	18.8	65.8	1 1.05
Case 27	Low Ind	Ceiling	15	18.8	65.8	12.31
Case 26	Slot	Ceiling	15	18.8	65.8	18.42

Volume II - Section IV - Whole Room Simulation Database

Page II - 55

4. WHOLE ROOM SIMULATION DATABASE

4.1 Description of Simulation Data Entry

The first page contains a listing of the room geometry and ventilation configuration together with a picture. Summary result data is then presented for both average rack and room breathing zones. Temperature and CO2 are presented as single figures whilst NH3 values are shown over a ten day period. Please refer to Volume I: Section 4. 1.2. 8. 2 for an explanation of the relationship between NH3 generation rates and cage relative humidity.

The second page shows three histogram population distributions of temperature, contamination (concentration) and relative humidity in the cage occupied zone of all cages in the room. The histograms show the number of cages (frequency) which have values that fall in the range between two values marked on the horizontal axis with the upper-value indicated. For example, in the sample histogram on the next page the column labeled 22.2 indicates that just over 40 cages have temperatures between 22°C and 22.2°C.

Volume II - 56

Ventilation Pesi2n Handbook on Animal Research Facilities Using Static Microisolators

Cascname BasecasC

Descriplion

				t'xhausi	Eihaust	"S		Room cage and ventilation
SuppK	Supply Discharge	Supply Discharge	I \haust
Configuration	Temperature (°C)	Rll	C ..nripii ati.m	Temperature (®0	RH			arrangement
Radial	18. »	61%	Ceiling				>
Change	Rack	Rack	Number of	Total mass of	Room
Station	Orientation	Density	Mice in Room	.NUce in R.K.m	Pressurisation
ON	On v\all	Single	loss	21100 gr	neg lOOcfm

Analysis Results

Cage Occupied Z^ine

	Tempenturc	CO.	RH
	“C		(ppm)
Mean	22 06	7! 70	2158	66 78%
SD	ruo	072	359	2 60®-'..
Max.	22 99	73 38	3133	75 U6“/.

Cage Occupied Zone NHj (ppm)

Dav	t	2	3	4	S	S	7	8	9	i9
Mean	1 15	2 18	3 37	6.33	10 of)	15.82	23 78	29 51	35.35	38.09
Max.	1 67	3.17	4 89	9 19	1547	22 96	34 53	42 84	51 32	55 30
Room Breathing Zone
	Teoste nature	CO,	RH
	»c		(ppn>
Mean	20 291	68 52	36	55.371',.
S.D	0.46	0 82	20
Max.	21 78	71 20	1.37
R(Him Breathing Zone	NHj (ppm)
Dsv	t	2	3	4	S		7	8	9	19
Mean	U.02	0t>4	OOh	0 1 1	0 18	0 2(5	0.4U	049	0 59	O.o3
Max.	0 07	0 14	021	0 41)	0.f)8	1 01	1 51	I 88	2 25	243

J

>

J

Cage occupied zone summary result data

Room breathing zone summary result data

15

10

Sample histrogram population distribution

Volume II - Section IV - Whole Room Simulation Database

Page 11-57

4.2 Simulation Data Entries

The following section presents data entries for each of the CFD animal research facility simulations.

Volume II - 58

Ventilation Design Handbook on Animal Research Facilities Using Static Microisolators

Casename BaSCCaSC

Description

Supply	Supply Discharge	Supply Discharge	Exhaust	Exhaust	Exhaust
Configuration	Temperature (°C)	RH	Configuration	Temperature (°C)	RH
Radial	18.8	61%	Ceiling	22	50%
Change	Rack	Rack	Number of	Total mass of	Room
Station	Orientation	Density	Mice in Room	Mice in Room	Pressurisation
ON	On wall	Single	1050	21000 gr	neg lOOcfm

Room

ACH

15

Cage

Condition

Top On

Cage Occupied Zone

Analysis Results

	Temperature	CO2	RH
	(ppm)
Mean	22.06 71.70	2T5S	0^ OC c
S.D.	0.40 0.72	559	2.60%
Max.	2199 73.38	3133	75.06%
Cage Occupied Zone NHj (ppm)
Day	I	2	3	5	5	6	7	8	9	TO
Mean	1.15	2.18	3.37	6.33	10.66	15.82	23.78	29.51	35.35	38.09
Max.	1.67	3.17	4.89	9t9	15.47	22.96	34.53	42.84	51.32	55.30

Room Breathing Zone

	Temperature	CO3,	RH
		T	(ppm)
Mean	20.291	68.52	36	55.37%
	0.46	0.82	20
Max.	21.78	71.20	137

Room Breathing Zone NHj (ppm)

Day	1	2	3	3	5	s	7	8	9	ID
Mean	0.02	0.04	0.06	0.1 1	0.18	0.26	0.40	0.49	0.59	(I63
Max.	0.07	0.14	on	0.40	0.68	1.01	1.51	1.88	2.25	2.43

Volume II - Section IV - Whole Room Simulation Database

Page II - 59

Histogram Distributions

Cage occupied zone average temperature (°C) distribution

Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors

(kg/kg ^ ppm)

Day	CO,	NH,
1	785000	418
2	785000	795
3	785000	1225
4	785000	1922
5	785000	2848
6	785000	3443
7	785000	8409
8	785000	8483
9	785000	8442
10	785000	11384

Cage occupied zone average relative humidity (%) distribution

Volume II - 60

Ventilation Design Handbook on Animal Research Facilities Using Static Microisolators

Casename Case 02

Description

Supply	Supply Discharge	Supply Discharge	Exhaust	Exhaust	Exhaust
Configuration	Temperature (°C)	RH	Configuration	Temperature (°C)	RH
Radial	18.8	61%	High	22	50%
Change	Rack	Rack	Number of	Total mass of	Room
Station	Orientation	Density	Mice in Room	Mice in Room	Pressurisation
ON	On wall	Single	1050	21000 gr	neg lOOcfm

Room

ACH

15

Cage

Condition

Top On

Analysis Results

Volume II - Section IV - Whole Room Simulation Database

Page 11-61

Histogram Distributions

Cage occupied zone average temperature (°C) distribution

Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors

(kg/kg ppm)

Day	CO,	NHj
1	785000	410
2	785000	795
5	785(XX)	1225
4	785(XX)	1860
5	785(XX)	TFTT
6	785000	3847
7	785000	5401
8	785(XK)	7122
9	785tXX)	9214
10	785(XX)	11384

Cage occupied zone average relative humidity (%) distribution

Volume II -62

Ventilation Design Handbook on Animal Research Facilities Using Static Microisolators

Casename Case 03

Description

Supply	Supply Discharge	Supply Discharge	Exhaust	Exhaust	Exhaust
Configuration	Temperature (°C)	RH	Configuration	Temperature (°C)	RH
Radial	18.8	61%	Low	22	50%
Change	Rack	Rack	Number of	Total mass of	Room
Station	Orientation	Density	Mice in Room	Mice in Room	Pressurisation
ON	On wall	Single	1050	21000 gr	neg lOOcfm

Room

ACH

15

Cage

Condition

Top On

Analysis Results

^agTn«:upietl Zone

	Temperature		RH —
	T	sp	(ppm)
Mean	22.06	71.71	64	49.73%
S.D.	0.37	0.66	25
Max.	23.37	74.07	170
Room Breathing Zone NH3 (ppm)

Day	i	2	3	3	5	6	7	S'	5	IG
Mean	0.03	0.06	0.10	0.14	0.20	0.28	(T3?i	0.52	(169	(I90
Max.	0.09	0.17	0.27	0.38	0.54	0.75	1.02	1.38	1.83	2.39

Volume II - Section IV - Whole Room Simulation Database

Page 11-63

Histogram Distributions

Cage occupied zone average temperature (°C) distribution

Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors

(kg/kg ppm)

Day	CO,	NII3
1	785000	408
2	785000	795
3	785000	1225
4	785000	1766
5	785000	2483
6	7850OO	3443
7	785000	4712
8	785000	6356
	785OO0	8442
10	785000	11384

Cage occupied zone average relative humidity (%) distribution

0

Volume II - 64

Ventilation Design Handbook on Animal Research Facilities Using Static Microisolators

Casename Case 04

Description

Supply	Supply Discharge	Supply Discharge	Exhaust	Exhaust	Exhaust
Configuration	Temperature (°C)	RH	Configuration	Temperature (°C)	RH
Slot	18.8	61%	Ceiling	22	50%
Change	Rack	Rack	Number of	Total mass of	Room
Station	Orientation	Density	Mice in Room	Mice in Room	Pressurisation
ON	On wall	Single	1050	21000 gr	neg lOOcfm

Room

ACH

15

Cage

Condition

Top On

7*ag?7yccupie^Ton^

Analysis Results

	Temperature	—	— RH —
	(ppm)
Mean	20.42 68.76	62	55.14%
S.D.	0.17 0.0	26
Max.	21.18 70.12	170
Room Breathing Zone NH3 (ppm)
Day	1	2	3	3	5	6	7	8	9	ID
VI can	o.o5	0.06	0.10	0.15	0.23	0.32	0.46	0.60	0.77	04
Max.	0.09	0.17	0.26	0.42	0^2	0:59	1.27	1.65	TTO	2.56

Volume II - Section IV - Whole Room Simulation Database

Page II - 65

Histogram Distributions

Cage occupied zone average temperature (°C) distribution

n n ri ri rt ri ri ri

Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors

(kg/kg ^ ppm)

Day	CO,	NHj
1	785000	411
2	785000	795
J	785000	1225
4	785000	1922
5	785000	2888
6	785000	4114
7	785000	5857
8	785000	7629
9	785000	9725
10	785000	11384

Cage occupied zone average relative humidity (%) distribution

Volume II - 66

Ventilation Design Handbook on Animal Research Facilities Using Static Microisolators

Casename Case 05

Description

Supply	Supply Discharge	Supply Discharge	Exhaust	Exhaust	Exhaust
Configuration	Temperature (°C)	RH	Configuration	Temperature (°C)	RH
Slot	18.8	61%	High	22	50%
Change	Rack	Rack	Number of	Total mass of	Room
Station	Orientation	Density	Mice in Room	Mice in Room	Pressurisation
ON	On wall	Single	1050	21000 gr	neg lOOcfm

Room

ACH

15

Cage

Condition

Top On

Cage Occupied Zone

Analysis Results

	Temperature	n p	RH
		op	(ppm)
Mean	212B	72.10	1742	62.51%
S.b.	0.25	0.45	276	2.09%
Max.	22.86	73.15	2333	67.21%

Cage Occupied Zone NH3 (ppm)

T5ay	1	2	3	4	5	5	7			10
Mean	0.91	1.76	2.72	4.23	6.32		12.75	16.66	TT.31	26.14
Max.	1.22	2.36	3.64	5.67	8.46	12.03	17.07	22.30	28.53	34.99

Room Breathing Zone

Volume II - Section IV - Whole Room Simulation Database

Page 11-67

Histogram Distributions

Cage occupied zone average temperature (°C) distribution

Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors

(kg/kg -> ppm)

Day	CO,	Ml3
1	785000	411
2	783000	795
3	785000	1225
4	785000	1906
5	785000	2848
6	785000	4048
7	785000	5744
a	785000	7504
9	785000	9599
10	785000	11384

Cage occupied zone average relative humidity (%) distribution

0

Volume II - 68

Ventilation Design Handbook on Animal Research Facilities Using Static Microisolators

Casename Case 06

Description

Supply	Supply Discharge	Supply Discharge	Exhaust	Exhaust	Exhaust
Configuration	Temperature (°C)	RH	Configuration	Temperature (°C)	RH
Slot	18.8	61%	Low	22	50%
Change	Rack	Rack	Number of	Total mass of	Room
Station	Orientation	Density	Mice in Room	Mice in Room	Pressurisation
ON	On wall	Single	1050	21000 gr	neg lOOcfm

Room

ACH

15

Cage

Condition

Top On

lied Zor

Analysis Results

Volume II - Section IV - Whole Room Simulation Database

Page II - 69

Histogram Distributions

Cage occupied zone average temperature (°C) distribution

Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors

(kg/kg ^ ppm)

Day	CO2	NH3
1	785000	408
2	785000	795
3	785000	1225
4	785000	1766
5	785000	2483
6	785000	3443
7	785000	4712
8	785000	6356
9	785000	8442
10	785000	11384

Cage occupied zone average relative humidity (%) distribution

Volume II - 70

Ventilation Design Handbook on Animal Research Facilities Using Static Microisolators

Casename Case 07

Description

Supply	Supply Discharge	Supply Discharge	Exhaust	Exhaust	Exhaust
Configuration	Temperature (°C)	RH	Configuration	Temperature (°C)	RH
Low Ind	18.8	61%	Ceiling	22	50%
Change	Rack	Rack	Number of	Total mass of	Room
Station	Orientation	Density	Mice in Room	Mice in Room	Pressurisation
ON	On wall	Single	1050	21000 gr	neg lOOcfm

Room

ACH

15

Cage

Condition

Top On

Cage Occupied Zone

Analysis Results

	Temperature	^0,	RH
^	(ppm)
Mean	19.96 67.93	57	56.73%
S.D.	0.68 1.22	82
Max.	2220 71.96	371
Room Breathing Zone NH3 (ppm)
15ay	i	2	3	3	5	5	7	g	5
Mean	0.03	0.06	0.09	0.16	0.27	0.41	0.61	0.76	(T9T	(199
Max.	0.20	0.38	0.58	1.07	1.79	2.65	3.97	T94	194	6.46

Volume II - Section IV - Whole Room Simulation Database

Page 11-71

Histogram Distributions

Cage occupied zone average temperature (°C) distribution

Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors

(kg/kg ^ ppm )

Day	COj	NH3
1	785000	418
1	785000	795
5	785000	1225
4	785000	2270
5	785000	3791
6	785000	5612
7	785000	8409
8	785000	10466
9	785000	12587
10	785000	11384

Cage occupied zone average relative humidity (%) distribution

Volume II - 72

Ventilation Design Handbook on Animal Research Facilities Using Static Microisolators

Casename	Case 08		Description
Supply	Supply Discharge	Supply Discharge Exhaust	Exhaust	Exhaust
Configuration	Temperature (°C)	RH	Configuration	Temperature (°C)	RH
Low Ind	18.8	61%	High	22	50%
Change	Rack	Rack	Number of	Total mass of	Room
Station	Orientation	Density	Mice in Room	Mice in Room	Pressurisation
ON	On wall	Single	1050	21000 gr	neg lOOcfm

Room

ACH

15

Cage

Condition

Top On

Tage Occupied Zone

Analysis Results

Volume II - Section IV - Whole Room Simulation Database

Page II - 73

Histogram Distributions

Cage occupied zone average temperature (°C) distribution

Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors

(kg/kg ^ ppm)

Day	CO,	NH3
1	785000	415
2	785000	795
3	785000	1225
4	785000	2027
5	785000	c^ 0
6	785000	4565
7	785000	6625
8	785000	8483
0	785000	105^6
10	785000	11384

Cage occupied zone average relative humidity (%) distribution

Volume II - 74

Ventilation Design Handbook on Animal Research Facilities Using Static Microisolators

Casename Case 09

Description

Supply	Supply Discharge	Supply Discharge	Exhaust	Exhaust	Exhaust
Configuration	Temperature (°C)	RH	Configuration	Temperature (°C)	RH
Low Ind	18.8	61%	Low	22	50%
Change	Rack	Rack	Number of	Total mass of	Room
Station	Orientation	Density	Mice in Room	Mice in Room	Pressurisation
ON	On wall	Single	1050	21000 gr	neg lOOcfm

Room

ACH

15

Cage

Condition

Top On

Cage Occupied Zone

upiec

Analysis Results

	Temperature	CO,	RH
^ cp	(ppm)
Mean	22.57 72 62	88	48.37%
S.b.	0.60 1.08	31
Max.	23.76 74.76	277
Room Breathing Zone NH3 (ppm)
Day	1		3	4	5	6	7	8	9	ID
Mean	0.05	0l)9	0.14	0.20	0.28	ojS	0.53	0.71	OO	1.23
Max.	0.14	OS	03	02	0.88	1.21	1.66	2.24	IM

Volume II - Section IV - Whole Room Simulation Database

Page I! - 75

Histogram Distributions

Cage occupied zone average temperature (°C) distribution

Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors

(kg/kg -> ppm)

Day	CO,	NHj
1	785000	408
2	785000	795
3	785000	1225
4	785000	1766
5	785000	2485
6	785(')0(:)	3443
7	785000	4712
H	785000	6356
0	785OO0	8442
10	785000	11384

Cage occupied zone average relative humidity (%) distribution

Volume II - 76

Ventilation Design Handbook on Animal Research Facilities Using Static Microisolators

Casename Case 10

Description

Supply	Supply Discharge	Supply Discharge	Exhaust	Exhaust	Exhaust
Configuration	Temperature (°C)	RH	Configuration	Temperature (°C)	RH
Radial	20.7	54%	Ceiling	22	50%
Change	Rack	Rack	Number of	Total mass of	Room
Station	Orientation	Density	Mice in Room	Mice in Room	Pressurisation
OFF	On wall	Single	1050	21000 gr	neg lOOcfm

Room

ACH

15

Cage

Condition

Top On

TagTCccupieS^onT'

Analysis Results

	Temperature	CO,	— RH —
		^F	(ppm)
Mean	20.92	69.66	29	53.13%
Js.b.	0.39	0.70	44
Max.	22.21	71.98	158

Room Breathing Zone NH3 (ppm)

bay	j	2	3	4	5	5	7	g	9	10
Mean	0.02	(I03	0.05	0.07	0.10	0.14	(Ti^	0:25	0.33	UaI
Max]	0.08	(Tre	0.25	0.37	0.53	0.75	1.05	1.39	1.81	T29

Volume II - Section IV - Whole Room Simulation Database

Page II - 77

Histogram Distributions

Cage occupied zone average temperature (°C) distribution

Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors

(kg/kg -> ppm)

Day	CO^	NHj
1	785000	410
2	785000	795
J	785000	1225
4	785000	l83i
5	785000	2655
6	785000	3728
7	785000	5148
8	785000	6897
0	785000	8987
10	785000	irm

Cage occupied zone average relative humidity (%) distribution

Volume II - 78

Ventilation Design Handbook on Animal Research Facilities Using Static Microisolators

Casename Case 11

Description

Supply	Supply Discharge	Supply Discharge	Exhaust	Exhaust	Exhaust
Configuration	Temperature (°C)	RH	Configuration	Temperature (°C)	RH
Radial	20.7	54%	Low	22	50%
Change	Rack	Rack	Number of	Total mass of	Room
Station	Orientation	Density	Mice in Room	Mice in Room	Pressurisation
OFF	On wall	Single	1050	21000 gr	neg lOOcfm

Room

ACH

15

Cage

Condition

Top On

(a ge Uccupiel^onr

Analysis Results

Volume II - Section IV - Whole Room Simulation Database

Page II - 79

Histogram Distributions

Cage occupied zone average temperature (°C) distribution

Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors

(kg/kg ^ ppm)

Day	CO,	NII3
1	7850OO	413
2	785000	795
3	785000	1225
4	785000	2008
5	7S500O	UTT
6	785000	4486
7	785000	6491
8	785000	8334
	785000	10436
10	785000	11384

Cage occupied zone average relative humidity (%) distribution

Volume II - 80

Ventilation Design Handbook on Animal Research Facilities Using Static Microisolators

Casename Case 12

Description

Supply	Supply Discharge	Supply Discharge	Exhaust	Exhaust	Exhaust
Configuration	Temperature (°C)	RH	Configuration	Temperature (°C)	RH
Slot	20.7	54%	Ceiling	22	50%
Change	Rack	Rack	Number of	Total mass of	Room
Station	Orientation	Density	Mice in Room	Mice in Room	Pressurisation
OFF	On wall	Single	1050	21000 gr	neg lOOcfm

Analysis Results

( age OccupTe^Ton?

	Temperature		RH
			(ppm)
.Mean	21^1	73.24	2208	63.65%
S.D.	07	07	m	2.7S%
Max.	UM	74.45	5024	69.07%

Cage Occupied Zone NH3 (ppm)

Hiy	1	2	3	3	5	5	7	8	9	lU
Mean	1.16	2.23	3.45	5.66	OS	12.66	18.32	23.52	29.43	34.67
Max.	1.59	3.06	4.72	7.75	rrui	17.33	25.09	32.20	40.30	47.47

Room Breathing Zone

Volume II - Section IV - Whole Room Simulation Database

Page II -81

Histogram Distributions

Cage occupied zone average temperature (°C) distribution

Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors

(kg/kg ^ ppm)

Day	CO2	NH3
1	785000	413
2	785000	795
5	785000	1225
4	785000	2011
5	785000	3121
6	785000	4500
7	785000	6514
8	785000	8360
9	7850()0	10462
to	785000	11384

Cage occupied zone average relative humidity (%) distribution

0

Volume II - 82

Ventilation Design Handbook on Animal Research Facilities Using Static Microisolators

Casename Case 13

Description

Supply	Supply Discharge	Supply Discharge	Exhaust	Exhaust	Exhaust
Configuration	Temperature (°C)	RH	Configuration	Temperature (°C)	RH
Slot	20.7	54%	Low	22	50%
Change	Rack	Rack	Number of	Total mass of	Room
Station	Orientation	Density	Mice in Room	Mice in Room	Pressurisation
OFF	On wall	Single	1050	21000 gr	neg lOOcfm

Room

ACH

15

Cage

Condition

Top On

Cage OccupTe^^onT

Analysis Results

Volume II - Section IV - Whole Room Simulation Database

Page II - 83

Histogram Distributions

Cage occupied zone average temperature (°C) distribution

Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors

(kg/kg -> ppm)

Day	COj	NHj
1	7S5MU	4l3
2	735000	795
3	785000	1225
4	735600	2273
5	785000	5798
6	785000	5624
7	785000	8430
8	785000	10490
*)	7350OO	13611
10	785000	11384

Cage occupied zone average relative humidity (%) di3tribution

Volume II - 84

Ventilation Design Handbook on Animal Research Facilities Using Static Microisolators

Casename Case 14

Description

Supply	Supply Discharge	Supply Discharge	Exhaust	Exhaust	Exhaust
Configuration	Temperature (°C)	RH	Configuration	Temperature (°C)	RH
Low Ind	20.7	54%	Ceiling	22	50%
Change	Rack	Rack	Number of	Total mass of	Room
Station	Orientation	Density	Mice in Room	Mice in Room	Pressurisation
OFF	On wall	Single	1050	21000 gr	neg lOOcfm

Room

ACH

15

Cage

Condition

Top On

T^ag^Ccajpie^^onT

Analysis Results

	Temperature	O p	— RH —
	(ppm)
Mean	21.12 70.01	52	52.68%
S.D.	0.17 0.31	23
Max.	22.07 71.72	220
Room Breathing Zone NH3 (ppm)
Day	1	2	3	5	5	5	7	8	9	ID
iVlean	0.03	0.05	(ToB	0.14	0.21	0.31	(O?	(OB	0.72	p bo
Max.	0.12	0.22	(04	0.58	0.91	1.32	1.93	2.45	3.04	5.52

Volume II - Section IV - Whole Room Simulation Database

Page II - 85

Histogram Distributions

Cage occupied zone average temperature (°C) distribution

Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors

(kg/kg ^ ppm)

Day	CO,	NH3
1	785000	414
2	785000	795
i	785000	1225
4	785000	2061
5	785000	3249
6	785O0O	4713
7	785000	6877
8	785000	8763
9	785000	10869
10	785000	11384

Cage occupied zone average relative humidity (%) distribution

0

Volume II - 86

Ventilation Design Handbook on Animal Research Facilities Using Static IMicroisolators

Casename Case 15

Description

Supply	Supply Discharge	Supply Discharge	Exhaust	Exhaust	Exhaust
Configuration	Temperature (°C)	RH	Configuration	Temperature (°C)	RH
Low Ind	20.7	54%	Low	22	50%
Change	Rack	Rack	Number of	fotal mass of	Room
Station	Orientation	Density	Mice in Room	Mice in Room	Pressurisation
OFF	On wall	Single	1050	21000 gr	neg lOOcfm

Room

ACH

15

Cage

Condition

Fop On

"^ag^ncajpiet^onT

Analysis Results

	Temperature	—	— RH —
T T	(ppm)
Mean	2331 73,84	TiO	61.71%
S.D.	08 (I5l	376	3.14%
Max.	23.88 74,98	3201	68.32%
Cage Occupied Zone NH3 (ppm)
Day	1	2	3	4	5	6	7	S	9
Mean	1.11	2.14	3.31	4,94	IM	10.06	14.03	18.61	24.25	30.72
Max.	1.67	3.24	5.00	7.47	10.83	15.20	21.20	28.12	36.65	46.42

Room Breathing Zone

	Temperature	co;	RH
	T		(ppm)
Mean	21.21	70.19	48	52.32%
S.D.	0.16	0.29	44
Max.	21.94	71.50	306

Room Breathing 2^ne NH3 (ppm)

Day	1	2	3	4	5	6	7	8	9	jg
Mean	0.03	0.05	0.07	0.1 1	0.16	0.23	0.32	0.42	0.55	otO
Max.		0.3 1	0.48	0.71	1.03	1.45	2.02	2.69	3.50	4.43

Volume II - Section IV - Whole Room Simulation Database

Page 11-87

Histogram Distributions

Cage occupied zone average temperature (°C) distribution

Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors

(kg/kg ^ ppm)

Day	CO,	NH3
1	785000	410
2	785000	795
3	785000	1225
4	785000	1832
5	78^000	2655
6	785000	3728
7	785000	5198
8	785000	6897
9	785000	8987
to	785000	11384

Cage occupied zone average relative humidity (%) distribution

Volume II - 88

Ventilation Design Handbook on Animal Research Facilities Using Static IMicroisolators

Casename Case 16

Description

Supply	Supply Discharge	Supply Discharge	Exhaust	Exhaust	Exhaust
Configuration	Temperature (°C)	RH	Configuration	Temperature (°C)	RH
Radial	18.8	61%	Ceiling	22	50%
Change	Rack	Rack	Number of	Total mass of	Room
Station	Orientation	Density	Mice in Room	Mice in Room	Pressurisation
ON	On wall	Single	1050	21000 gr	pos lOOcfm

Room

ACH

15

Cage

Condition

Top On

Analysis Results

	Temperature	CO,	— RH —
0 n 0 T!	(ppm)
Mean	20.07 68.12	37	56.18%
S.D.	129 2.33	47
Max.	23.06 73.52	251
Room Breathing Zone NH3 (ppm)
Day	I	2	3	4	5	6	7	g	g	10
Mean	0.02	OT0	0.06	0.10	0.15	0.22	0.31	DaD	(I50	DIE
Max.	0.13	0.25	0.3^)	0.65	1.02	T4S	2.16	2.76	3.43	5I5D

Volume II - Section IV - Whole Room Simulation Database

Page 11-89

Histogram Distributions

Cage occupied zone average temperature (°C) distribution

Cage occupied zone average contamination (kg/kg) distribution

Cage occupied zone average relative humidity (%) distribution

Contamination conversion factors (kg/kg ppm)

Volume II - 90

Ventilation Design Handbook on Animal Research Facilities Using Static Microisolators

Casename Case 17

Description

Supply	Supply Discharge	Supply Discharge	Exhaust	Exhaust	Exhaust
Configuration	Temperature (°C)	RH	Configuration	Temperature (°C)	RH
Slot	18.8	61%	Ceiling	22	50%
Change	Rack	Rack	Number of	Total mass of	Room
Station	Orientation	Density	Mice in Room	Mice in Room	Pressurisation
ON	On wall	Single	1050	21000 gr	pos lOOcfm

Room

ACH

15

Cage

Condition

Top On

Analysis Results

Volume II - Section IV - Whole Room Simulation Database

Page II - 91

Histogram Distributions

Cage occupied zone average temperature (°C) distribution

Cage occupied zone average contamination (kg/kg) distribution

Cage occupied zone average relative humidity (%) distribution

Contamination conversion factors (kg/kg ^ ppm)

Volume II - 92

Ventilation Design Handbook on Animal Research Facilities Using Static Microisolators

Casename Case 18

Description

Supply	Supply Discharge	Supply Discharge	Exhaust	Exhaust	Exhaust
Configuration	Temperature (°C)	RH	Configuration	Temperature (°C)	RH
Low Ind	18.8	61%	Ceiling	22	50%
Change	Rack	Rack	Number of	Total mass of	Room
Station	Orientation	Density	Mice in Room	Mice in Room	Pressurisation
ON	On wall	Single	1050	21000 gr	pos lOOcfm

Room

ACH

15

Cage

Condition

Top On

Cage OccupIecTZonT

Analysis Results

Volume II - Section IV - Whole Room Simulation Database

Page 11-93

Histogram Distributions

Cage occupied zone average temperature (°C) distribution

Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors

(kg/kg ppm)

Day	COj	NHj
i	785000	413
2	785000	795
3	785000	1225
4	785000	2024
5	785000	3152
6	785000	4552
7	785000	6603
8	785000	8458
*)	785000	10562
10	785000	11384

Cage occupied zone average relative humidity (%) distribution

Volume II - 94

Ventilation Design Handbook on Animal Research Facilities Using Static Microisolators

Casename Case 19

Description

Supply	Supply Discharge	Supply Discharge	Exhaust	Exhaust	Exhaust
Configuration	Temperature (°C)	RH	Configuration	Temperature (°C)	RH
Radial	18.8	61%	Ceiling	22	50%
Change	Rack	Rack	Number of	Total mass of	Room
Station	Orientation	Density	Mice in Room	Mice in Room	Pressurisation
ON	Perp	Single	1050	21000 gr	neg lOOcfm

Room

ACH

15

Cage

Condition

Top On

^tge^orupied z!one

Analysis Results

Volume II - Section IV - Whole Room Simulation Database

Page II - 95

Histogram Distributions

Cage occupied zone average temperature (°C) distribution

Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors

(kg/kg ^ ppm)

Day	COj	NHj
1	785000	415
2	785000	795
5	785000	1225
4	785000	2100
5	785000	~UW
6	785000	4879
7	785000	7160
8	785000	9078
f)	785000	1 1186
10	785000	TilP"

Cage occupied zone average relative humidity (%) distribution

0

Volume II - 96

Ventilation Design Handbook on Animal Research Facilities Using Static Microisolators

Casename Case 20

Description

Supply	Supply Discharge	Supply Discharge	Exhaust	Exhaust	Exhaust
Configuration	Temperature (°C)	RH	Configuration	Temperature (°C)	RH
Slot	18.8	61%	Ceiling	22	50%
Change	Rack	Rack	Number of	Total mass of	Room
Station	Orientation	Density	Mice in Room	Mice in Room	Pressurisation
ON	Perp	Single	1050	21000 gr	neg lOOcfm

Room

ACH

15

Cage

Condition

Top On

Cage OccupTetTZonT

Analysis Results

Volume II - Section IV - Whole Room Simulation Database

Page II - 97

Histogram Distributions

Cage occupied zone average temperature (°C) distribution

Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors

(kg/kg ^ ppm)

Day	COj	NH3
1	785000	411
2	785000	795
3	785000	1225
4	785O0O	1915
5	785000	"?S'7'1 '
6	785000	4087
7	785000	5810
	785000	7576
*)	785000	9672
10	785000	1 1384

Cage occupied zone average relative humidity (%) distribution

0

Volume II - 98

Ventilation Design Handbook on Animal Research Facilities Using Static Microisolators

Casename Case 21

Description

Supply	Supply Discharge	Supply Discharge	Exhaust	Exhaust	Exhaust
Configuration	Temperature (°C)	RH	Configuration	Temperature (°C)	RH
Low Ind	18.8	61%	Ceiling	22	50%
Change	Rack	Rack	Number of	Total mass of	Room
Station	Orientation	Density	Mice in Room	Mice in Room	Pressurisation
ON	Perp	Single	1050	21000 gr	neg lOOcfm

Room

ACH

15

Cage

Condition

Top On

Analysis Results

Cage OccupieT^n^””

Volume II - Section IV - Whole Room Simulation Database

Page 11-99

Histogram Distributions

Cage occupied zone average temperature (°C) distribution

Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors

(kg/kg ^ ppm)

Day	CO,	NHj
1	7k5000	414
2	785000	795
3	785000	1225
4	785000	:>054
5	785000
6	785000	4683
7	785000	6826
a	785000	8706
*)	785000	10811
10	785000	11384

Cage occupied zone average relative humidity (%) distribution

Volume II - 100

Ventilation Design Handbook on Animal Research Facilities Using Static Microisolators

Casename Case 22

Description

Supply	Supply Discharge	Supply Discharge	Exhaust	Exhaust	Exhaust
Configuration	Temperature (°C)	RH	Configuration	Temperature (°C)	RH
Radial	18.8	61%	Low	22	50%
Change	Rack	Rack	Number of	Total mass of	Room
Station	Orientation	Density	Mice in Room	Mice in Room	Pressurisation
ON	Perp	Single	1050	21000 gr	neg lOOcfm

Room

ACH

15

Cage

Condition

Top On

(L'age ^iccupied Zone

Analysis Results

	Temperature		RH
5^ - Cp	(ppm)
Mean	21.24 70.24	61	52.34%
S.b.	0.71 1.27	28
Max.	26:S2 80.28	217
Room Breathing Zone NH3 (ppm)
Day	1	2	3	4		6	7	8		10
Mean	0.03	0.06	0.10	0.14	0.20	0.27	0.37	0.50	0.66	05
Max.	0.11	0.22	0.34	0.49	0.69	0.97	1.33	1.78	2.36	05

Volume II - Section IV - Whole Room Simulation Database

Page II - 101

Histogram Distributions

Cage occupied zone average temperature (°C) distribution

Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors

(kg/kg ^ ppm)

Day	COj	NHj
1	785000
2	785000	705
3	785000	1225
4	785000	1779
5	785000	5516
6	785000	3498
7	785000	4807
8	785000	6461
9	785000	8548
10	785000	11384

Cage occupied zone average relative humidity (%) distribution

Volume II - 102

Ventilation Design Handbook on Animal Research Facilities Using Static Microisolators

Casename Case 23

Description

Supply	Supply Discharge	Supply Discharge	Exhaust	Exhaust	Exhaust
Configuration	Temperature (°C)	RH	Configuration	Temperature (°C)	RH
Slot	18.8	61%	Low	22	50%
Change	Rack	Rack	Number of	Total mass of	Room
Station	Orientation	Density	Mice in Room	Mice in Room	Pressurisation
ON	Perp	Single	1050	21000 gr	neg lOOcfm

Room

ACH

15

Cage

Condition

Top On

Analysis Results

	Temperature	—TUT	— RH —
			(ppm)
Mean		72.86	1^52	60.16%
S.D.	(ITt	0.85	375	2.57%
Max.	2535	74.38	2552	66.04%
Cage Occupied Zone NH3 (ppm)

T5ay	1	2	3	3	5^	5	7	S	5	TO j
Mean	P bo O'.	1.67	2.58	3.71	5.22	7.24	Wi	13.37	17.76	23.22
Max.	1.35	23E	5.08	5.74	837	11.19	15.32	20.67	27.45	35.88

Room Breathing Zone

Volume II - Section IV - Whole Room Simulation Database

Page II - 103

Histogram Distributions

Cage occupied zone average temperature (°C) distribution

Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors

(kg/kg ^ ppm)

Day	CO,	NHj
1	785000	408
2	7})5000	705
3	7850OO	\ns
4	785000	1766
5	785000	"ITST
6	7850O0	3445
7	7850OO	4712
H	785000	6356
!)	785000	8442
10	785000	11384

Cage occupied zone average relative humidity (%) distribution

0

Volume II - 104

Ventilation Design Handbook on Animal Research Facilities Using Static Microisolators

Casename Case 24

Description

Supply	Supply Discharge	Supply Discharge	Exhaust	Exhaust	Exhaust
Configuration	Temperature (°C)	RH	Configuration	Temperature (°C)	RH
Low Ind	18.8	61%	Low	22	50%
Change	Raek	Rack	Number of	Total mass of	Room
Station	Orientation	Density	Mice in Room	Mice in Room	Pressurisation
ON	Perp	Single	1050	21000 gr	neg lOOcfm

Room

ACH

15

Cage

Condition

Top On

Cage Occupied Zone

Analysis Results

	Temf^rature	—CO—	RH
^ —sp	(ppm)
Mean	22.10 71.78	81	49,75%
S.D.	(Tts in	47
Max.	TTtS 82.00	W
Room Breathing Zone NH3 (ppm)
Day	^71	2	3	4		6	7	8	g— -
Mean		O b 00	0.13	oTS	0.26	0.36	0.49	0.66	OS	1.15
Max.	0.22	0.42	0.67	0.06	1.35	1.87	2.56	3.46	430	on

Volume II - Section IV - Whole Room Simulation Database

Page 11-105

Histogram Distributions

Cage occupied zone average temperature (°C) distribution

Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors

(kg/kg -> ppm)

Day	COj	NHj
1	785000	o 00
2	785O00	795
i	785000	1225
4	785000	1766
5	785000	2483
6	785000	3443
7	785000	4712
8	785000	6356
*)	785000	8442
10	785000	11384

Cage occupied zone average relative humidity (%) distribution

Volume II - 106

Ventilation Design Handbook on Animal Research Facilities Using Static Microisolators

Casename	Case 25		Description
Supply	Supply Discharge	Supply Discharge Exhaust	Exhaust	Exhaust
Configuration	Temperature (°C)	RH	Configuration	Temperature (°C)	RH
Radial	18.8	61%	Ceiling	22	50%
Change	Rack	Rack	Number of	Total mass of	Room
Station	Orientation	Density	Mice in Room	Mice in Room	Pressurisation
ON	On wall	Single	1050	21000 gr	neg lOOcfm

Room

ACH

15

Cage

Condition

Sealed

TagiTncoIpie^^onr

Analysis Results

Volume II - Section IV - Whole Room Simulation Database

Page 11-107

Histogram Distributions

Cage occupied zone average temperature (°C) distribution

Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors

(kg/kg ^ ppm)

Day	COj	NHj
1	785000	429
2	785000	795
3	785000	1225
4	785000	2885
5	785000	5386
6	78500O	8256
7	785000	129lil
8	785000	15479
0	7^5000	17641
It)	785000	11384

Cage occupied zone average relative humidity (%) distribution

Volume II - 108

Ventilation Design Handbook on Animal Research Facilities Using Static Microisolators

Casename Case 26

Description

Supply	Supply Discharge	Supply Discharge	Exhaust	Exhaust	Exhaust
Configuration	Temperature (°C)	RH	Configuration	Temperature (°C)	RH
Slot	18.8	61%	Ceiling	22	50%
Change	Rack	Rack	Number of	Total mass of	Room
Station	Orientation	Density	Mice in Room	Mice in Room	Pressurisation
ON	On wall	Single	1050	21000 gr	neg lOOcfm

Room

ACH

15

Cage

Condition

Sealed

TagTOccupieTZon^

Analysis Results

Volume II - Section IV - Whole Room Simulation Database

Page II - 109

Histogram Distributions

Cage occupied zone average temperature (°C) distribution

Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors

(kg/kg ppm)

Day	CO2	NII3
1	785000	443
2	785000	795
3	785000	1225
4	785000	3639
5	785000	7341
6	785000	1 1497
7	785000	18443
8	785000	21621
9	785000	23835
10	785000	11384

Cage occupied zone average relative humidity (%) distribution

Volume II - 110

Ventilation Design Handbook on Animal Research Facilities Using Static Microisolators

Casename Case 27

Description

Supply	Supply Discharge	Supply Discharge	Exhaust	Exhaust	Exhaust
Conflguration	Temperature (°C)	RH	Configuration	Temperature (°C)	RH
Low Ind	18.8	61%	Ceiling	22	50%
Change	Rack	Rack	Number of	Total mass of	Room
Station	Orientation	Density	Mice in Room	Mice in Room	Pressurisation
ON	On wall	Single	1050	21000 gr	neg lOOcfm

Room

ACH

15

Cage

Condition

Sealed

Cage OccupIeTTonr"

Analysis Results

	Temperature	CO,	— mr~
^ !5p	(ppm)
Mean	20.31 68.56	61	55.54%
S.b.	0:25 045	26
Max.	21.95 71.51	230
Room Breathing Zone NH3 (ppm)
Day	1	2	3	4	5	6	7	8	9	10
Mean	0151	0.06	OTO	013	015	010	1.10	1.31	1.48	1.10
Max.	0.13	013	0.37	— oir~	1.77	2.72	310	6A\	5.77	510

Volume II - Section IV - Whole Room Simulation Database

Page II- III

Histogram Distributions

Cage occupied zone average temperature (°C) distribution

Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors

(kg/kg ppm)

Day	o o	NHj
1	785000	432
2	785000	795
3	785000	1225
4	785000	3049
5	785000	5811
6	785000	8960
7	785000	14119
8	785000	16814
9	785000	18988
10	785000	1 1384

Cage occupied zone average relative humidity (%) distribution

Volume 11-112

Ventilation Design Handbook on Animal Research Facilities Using Static Microisolators

Casename Case 28

Description

Supply

Conflguration

Low Ind

Supply Discharge Temperature (°C) 16.8

Supply Discharge RH

69%

Exhaust

Configuration

Low

Exhaust Exhaust

Temperature (°C) RH

22 50%

Change

Station

ON

Rack	Rack	Number of	Total mass of	Room
Orientation	Density	Mice in Room	Mice in Room	Pressurisation
On wall	Single	1050	21000 gr	neg lOOcfm

Room

ACH

10

Cage

Condition

Top On

Cage Occupied Zone

Analysis Results

	Temj»rature	CO,	RH
— —	(ppm)
Mean	22.28 72.10	113	49.45%
S.D.	(151 LTT)	29
Max.	531S 74.09	307
Room Breathing Zone NH3 (ppm)
Day			3	4. ;	:■ .-,5. :7,		7	S
Mean	0.06	im	O.U(	(Il5	T05		(T5S		nT~^	1.59
Max.		O.M	0.4S	0.69	0.97	1.35	1.84	— mr-	— 05“	4.31

Volume II - Section IV - Whole Room Simulation Database

Page II- 113

Histogram Distributions

Cage occupied zone average temperature (°C) distribution

Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors

(kg/kg ^ ppm)

Day	CO2	NHj
1	785000	408
2	785000	795
3	785000	1225
4	785000	1766
5	785000	2483
6	785000	3443
7	785000	4712
8	785000	6356
9	785000	8442
10	785000	1 1384

0

Volume 11-114

Ventilation Design Handbook on Animal Research Facilities Using Static Microisolators

Casename Case 29

Description

Supply	Supply Discharge	Supply Discharge	Exhaust	Exhaust	Exhaust
Configuration	Temperature (°C)	RH	Configuration	Temperature (°C)	RH
Low Ind	11	65%	Low	22	50%
Change	Rack	Rack	Number of	Total mass of	Room
Station	Orientation	Density	Mice in Room	Mice in Room	Pressurisation
ON	On wall	Single	1050	21000 gr	neg lOOcfm

Room

ACH

5

Cage

Condition

Top On

7ageT5ccupie^^onT

Analysis Results

Volume II - Section IV - Whole Room Simulation Database

Page II - 1 15

Histogram Distributions

Cage occupied zone average temperature (°C) distribution

Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors

(kg/kg ^ ppm)

Day	COj	NHj
1	785000	408
2	785000	795
3	7^5000	|755
4	785000	1766
5	785000	2483
6	785000	3443
7	785000	4712
	7^5000	6356
9	7J)5000	§442
10	785000	11384

Cage occupied zone average relative humidity (%) distribution

Volume 11-116

Ventilation Design Handbook on Animal Research Facilities Using Static Micro iso la tors

Casename Case 30

Description

Supply	Supply Discharge	Supply Discharge	Exhaust	Exhaust	Exhaust
Configuration	Temperature (°C)	RH	Configuration	Temperature (°C)	RH
Low Ind	19.8	57%	Low	22	50%
Change	Rack	Rack	Number of	Total mass of	Room
Station	Orientation	Density	Mice in Room	Mice in Room	Pressurisation
ON	On wall	Single	1050	21000 gr	neg lOOcfm

Room

Acn

20

Cage

Condition

Top On

Tag^Hccupie^^on^

Analysis Results

Volume II - Section IV - Whole Room Simulation Database

Page II- 117

Histogram Distributions

Cage occupied zone average temperature (°C) distribution

Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors

(kg/kg ^ ppm)

Day	COj	NH3
1	785000	408
2	785000	795
3	785000	\115
4	785000	1766
5	7ii5oOO	2485
6	785000	3443
7	785000	4712
ii	785000	6356
9	785000	8442
10	7!)5000	11384

Cage occupied zone average relative humidity (%) distribution

Volume II - 1 18

Ventilation Design Handbook on Animal Research Facilities Using Static Microisolators

Casename Case 31

Description

Supply	Supply Discharge	Supply Discharge	Exhaust	Exhaust	Exhaust
Configuration	Temperature (°C)	RH	Configuration	Temperature (°C)	RH
Radial	17.5	66%	Low	22	50%
Change	Rack	Rack	Number of	Total mass of	Room
Station	Orientation	Density	Mice in Room	Mice in Room	Pressurisation
ON	Perp	Double	2100	42000 gr	neg lOOcfm

Analysis Results

7age^ccupTeT5Ionr

Volume II - Section IV - Whole Room Simulation Database

Page II - 119

Histogram Distributions

Cage occupied zone average temperature (°C) distribution

(N (N <N (N C'l (N CN

Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors

(kg/kg ^ ppm)

Day	CO,	NHj
1	785000	400
2	7850OO	795
3	785000	1225
4	785000	1805
5	785000	2579
6	785000	1MT
7	785000	4984
n	785000	6658
9	785000	8747
10	785000	11384

Cage occupied zone average relative humidity (%) distribution

Volume II - 120

Ventilation Design Handbook on Animal Research Facilities Using Static Microisolators

Casename Case 32

Description

Supply	Supply Discharge	Supply Discharge	Exhaust	Exhaust	Exhaust
Configuration	Temperature (°C)	RH	Configuration	Temperature (°C)	RH
Slot	17.5	66%	Low	22	50%
Change	Rack	Rack	Number of	Total mass of	Room
Station	Orientation	Density	Mice in Room	Mice in Room	Pressurisation
ON	Perp	Double	2100	42000 gr	neg lOOcfm

Analysis Results

TagTCccupIecTTonr

Volume II - Section IV - Whole Room Simulation Database

Page II - 121

Histogram Distributions

Cage occupied zone average temperature (°C) distribution

Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors

(kg/kg ^ ppm)

Day	COj	NHj
1	785000	408
2	785000	795
5	7S5000	1:)25
4	7k5000	1766
5	785O00	2483
6	785000	3443
7	785000	4712
8	TFSUDO	6356
9	785000	844:)
10	785000	11384

Cage occupied zone average relative humidity (%) distribution

Volume II - 122

Ventilation Design Handbook on Animal Research Facilities Using Static Microisolators

Casename Case 33

Description

Supply	Supply Discharge	Supply Discharge	Exhaust	Exhaust	Exhaust
Configuration	Temperature (°C)	RH	Configuration	Temperature (°C)	RH
Low Ind	17.5	66%	Low	22	50%
Change	Rack	Rack	Number of	Total mass of	Room
Station	Orientation	Density	Mice in Room	Mice in Room	Pressurisation
ON	Perp	Double	2100	42000 gr	neg lOOcfm

Analysis Results

T’ag^nccupie^^onT

Volume II - Section IV - Whole Room Simulation Database

Page II - 123

Histogram Distributions

Cage occupied zone average temperature (°C) distribution

Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors

(kg/kg ^ ppm)

Day	COj	NHj
1	785O00	408
2	785000	795
3	785000	1225
4	785OO0	1766
5	785O00	2483
6	785000	3441'
7	785000	4712
it	785000	6356
9	785O00	8442
10	785000	11384

Cage occupied zone average relative humidity (%) distribution

Volume II -124

Ventilation Design Handbook on Animal Research Facilities Using Static Microisolators

Casename Case 34

Description

Supply	Supply Discharge	Supply Discharge	Exhaust	Exhaust	Exhaust
Configuration	Temperature (°C)	RH	Configuration	Temperature (°C)	RH
Radial	17,5	66%	Ceiling	22	50%
Change	Rack	Rack	Number of	Total mass of	Room
Station	Orientation	Density	Mice in Room	Mice in Room	Pressurisation
ON	Perp	Double	2100	42000 gr	neg lOOcfm

Analysis Results

Cage Occupied Zone

Volume II - Section IV - Whole Room Simulation Database

Page 11-125

Histogram Distributions

Cage occupied zone average temperature (°C) distribution

Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors

(kg/kg -> ppm)

Day	COj	NH3
1	785000	410
2	785000	795
3	785000	1755
4	785000	2346
5	785000	im'
6	785000
7	7S5000	8066
8	785000	11085
9	785000	13210
10	785000	11384

Cage occupied zone average relative humidity (%) distribution

Volume II -126

Ventilation Design Handbook on Animal Research Facilities Using Static Microisolators

Casename Case 35

Description

Supply	Supply Discharge	Supply Discharge	Exhaust	Exhaust	Exhaust
Configuration	Temperature (°C)	RH	Configuration	Temperature (°C)	RH
Slot	17,5	66%	Ceiling	22	50%
Change	Rack	Rack	Number of	Total mass of	Room
Station	Orientation	Density	Mice in Room	Mice in Room	Pressurisation
ON	Perp	Double	2100	42000 gr	neg lOOcfm

Room

ACH

15

Cage

Condition

Top On

Tage^ccupietT^onT

Analysis Results

	Temf^rature	CO,	RH
T ^	(ppm)
Mean	19.96 67.92	122	57.37%
S.D.	1.01 l.$2	50
Max.	78.67	201
Room Breathing Zone NH3 (ppm)
Day	1	2	3		5	6	7	8
Mean	0.06	(Til	OTO	01^	035	032	1.21	1.52	T35	2.06
Max.	0.15	0.20	0.45	0.81	1.32	1.05	2.80	150	53T

Volume II - Section IV - Whole Room Simulation Database

Page 11-127

Histogram Distributions

Cage occupied zone average temperature (°C) distribution

Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors

(kg/kg ^ ppm)

Day	COj	NHj
1	785000	416
1	785000	~1W
3	785000	1225
4	785000	2185
5	785000	3571
6	785000	5246
7	7850OO	7787
H	785000	9774
	785000	118^9
10	7})5O00	11384

Cage occupied zone average relative humidity (%) distribution

Volume 11-128

Ventilation Design Handbook on Animal Research Facilities Using Static Microisolators

Casename Case 36

Description

Supply	Supply Discharge	Supply Discharge	Exhaust	Exhaust	Exhaust
Configuration	Temperature (°C)	RH	Configuration	Temperature (°C)	RH
Low Ind	17.5	66%	Ceiling	22	50%
Change	Rack	Rack	Number of	Total mass of	Room
Station	Orientation	Density	Mice in Room	Mice in Room	Pressurisation
ON	Petp	Double	2100	42000 gr	neg lOOcfm

Room

ACH

15

Cage

Condition

Top On

^'age O caipie^Ton^

Analysis Results

Volume II - Section IV - Whole Room Simulation Database

Page II - 129

Histogram Distributions

Cage occupied zone average temperature (°C) distribution

Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors

(kg/kg -> ppm)

Day	COj	NHj
I	7i)5000	418
2	785000	795
3	7MDI5U	~T7IT
4	785000	2274
5	785000	3802
6	785000	5629
7	785000	8450
	785O00	10500
9	785000	12620
10	785000	11384

Cage occupied zone average relative humidity (%) distribution

Volume II -130

Ventilation Design Handbook on Animal Research Facilities Using Static Microisolators

Casename Case 37

Description

Supply	Supply Discharge	Supply Discharge	Exhaust	Exhaust	Exhaust
Configuration	Temperature (°C)	RH	Configuration	Temperature (°C)	RH
Radial	18.8	61%	Ceiling/Low 50/50	22	50%
Change	Rack	Rack	Number of	Total mass of	Room
Station	Orientation	Density	Mice in Room	Mice in Room	Pressurisation
ON	On wall	Single	1050	21000 gr	neg lOOcfm

Room

ACH

15

Cage

Condition

Top On

^ag^HmipIe^Ton^

Analysis Results

Volume II - Section IV - Whole Room Simulation Database

Page II - 131

Histogram Distributions

Cage occupied zone average temperature (°C) distribution

Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors

(kg/kg ^ ppm)

Day	CO,	NHj
1	785000	411
2	785000	795
3	785000	1225
4	785000	1891
5	7H5O00	2808
6	785000	3983
7	785000	5632
8	785000	7379
	7^<50()()	9473
10	785000	11384

Cage occupied zone average relative humidity (%) distribution

Volume II -132

Ventilation Design Handbook on Animal Research Facilities Using Static Microisolators

Casename Case 38

Description

Supply	Supply Discharge	Supply Discharge	Exhaust	Exhaust	Exhaust
Configuration	Temperature (°C)	RH	Configuration	Temperature (°C)	RH
Slot	18.8	61%	Ceiling/Low 50/50	22	50%
Change	Rack	Rack	Number of	Total mass of	Room
Station	Orientation	Density	Mice in Room	Mice in Room	Pressurisation
ON	On wall	Single	1050	21000 gr	neg lOOcfm

Room

ACH

15

Cage

Condition

Top On

Cage OccupieT^one"

Analysis Results

Volume II - Section IV - Whole Room Simulation Database

Page II - 133

Histogram Distributions

Cage occupied zone average temperature (°C) distribution

Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors

(kg/kg ^ ppm)

Day	COj	NHj
1		411
2	785000	795
3	785O0O	1725
4	785000	1807
5	785000	2811
6	785000	3086
7	785000	5630
s	785O00	7386
9	785000	9481
10	785000	11384

Cage occupied zone average relative humidity (%) distribution

Volume II -134

Ventilation Design Handbook on Animal Research Facilities Using Static Microisolators

Casename Case 39

Description

Supply	Supply Discharge	Supply Discharge	Exhaust	Exhaust	Exhaust
Configuration	Temperature (°C)	RH	Configuration	Temperature (°C)	RH
Low Ind	18.8	61%	Ceiling/Low 50/50	22	50%
Change	Rack	Rack	Number of	Total mass of	Room
Station	Orientation	Density	Mice in Room	Mice in Room	Pressurisation
ON	On wall	Single	1050	21000 gr	neg lOOcfm

Room

ACH

15

Cage

Condition

Top On

7ag^5ccupie^^one

Analysis Results

Volume II - Section IV - Whole Room Simulation Database

Page II - 135

Histogram Distributions

Cage occupied zone average temperature (°C) distribution

Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors

(kg/kg ^ ppm)

Day	COj	NHj
i	785000	4l7
2	785000	795
3	785000	15:55
4	785000	1044
5	7i)500O	5047
6	785000	4515
7	785000	6023
K	785000	781 5
9	785000	09l 1
to	785000	THM

Cage occupied zone average relative humidity (%) distribution

0

Volume II -136

Ventilation Design Handbook on Animal Research Facilities Using Static Microisolators

Casename Case 40

Description

Supply	Supply Discharge	Supply Discharge	Exhaust	Exhaust	Exhaust
Configuration	Temperature (°C)	RH	Conflguration	Temperature (°C)	RH
Radial	18.8	61%	Ceiling x4	22	50%
Change	Rack	Rack	Number of	Total mass of	Room
Station	Orientation	Density	Mice in Room	Mice in Room	Pressurisation
ON	On wall	Single	1050	21000 gr	neg lOOcfm

Room

ACH

15

Cage

Condition

Top On

Tag^Cmipie^^on^

Analysis Results

Volume II - Section IV - Whole Room Simulation Database

Page II - 137

Histogram Distributions

Cage occupied zone average temperature (°C) distribution

Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors

(kg/kg ^ ppm)

Day	COj	NHj
1	785000	415
2	785000	705
i	785000	1225
4	785000	2118
5	785000	3396
6	785000	4957
7	785000	7504
8	785000	9257
9	785000	11537
10	785000	11384

Cage occupied zone average relative humidity (%) distribution

Volume II -138

Ventilation Design Handbook on Animal Research Facilities Using Static Microisolators

Casename	Case 41		Description
Supply	Supply Discharge	Supply Discharge Exhaust	Exhaust	Exhaust
Configuration	Temperature (°C)	RH	Configuration	Temperature (°C)	RH
Slot	18.8	61%	Ceiling x4	22	50%
Change	Rack	Rack	Number of	Total mass of	Room
Station	Orientation	Density	Mice in Room	Mice in Room	Pressurisation
ON	On wall	Single	1050	21000 gr	neg lOOcfm

Room

ACH

15

Cage

Condition

Top On

Cage Occupied Zone

Analysis Results

Volume II - Section IV - Whole Room Simulation Database

Page II - 139

Histogram Distributions

Cage occupied zone average temperature (°C) distribution

Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors

(kg/kg ^ ppm)

Day	COj	NHj
1	78500()	411
2	785000	795
3	785000	1225
4	755UM	18f)5
5	785000	2701
6	785000	3054
7	785000	5584
&	785000	7325
	785000	9419
10	785000	11384

Cage occupied zone average relative humidity (%) distribution

Volume II -140

Ventilation Design Handbook on Animal Research Facilities Using Static Microisolators

Casename Case 42

Description

Supply	Supply Discharge	Supply Discharge	Exhaust	Exhaust	Exhaust
Configuration	Temperature (°C)	RH	Configuration	Temperature (°C)	RH
Low Ind	18.8	61%	Ceiling x4	22	50%
Change	Rack	Rack	Number of	Total mass of	Room
Station	Orientation	Density	Mice in Room	Mice in Room	Pressurisation
ON	On wall	Single	1050	21000 gr	neg lOOcfm

Room

ACH

15

Cage

Condition

Top On

Tag^Occupie^^o

upiec

Analysis Results

Volume II - Section IV - Whole Room Simulation Database

Page II - 141

Histogram Distributions

Cage occupied zone average temperature (°C) distribution

Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors

(kg/kg ^ ppm)

Day	COj	NHj
1	7850OO	411
1	785000	705
3	785000	1225
4	785000	1930
5	7S5000	2910
6	785000	4151
7	78500O	5910
»	785000	769k
	785000	TJW
10	7850O0	1 lt§4

Cage occupied zone average relative humidity (%) distribution

Volume II -142

Ventilation Design Handbook on Animal Research Facilities Using Static Microisolators

Casename Case 43

Description

Supply	Supply Discharge	Supply Discharge	Exhaust	Exhaust	Exhaust
Configuration	Temperature (°C)	RH	Configuration	Temperature (°C)	RH
Low Ind (rot 90°)	18.8	61%	Ceiling	22	50%
Change	Rack	Rack	Number of	Total mass of	Room
Station	Orientation	Density	Mice in Room	Mice in Room	Pressurisation
ON	On wall	Single	1050	21000 gr	neg lOOcfm

Room

ACH

15

Cage

Condition

Top On

Cage OmjpIeTTonT

Analysis Results

Volume II - Section IV - Whole Room Simulation Database

Page 11-143

Histogram Distributions

Cage occupied zone average temperature (°C) distribution

Cage occupied zone average contamination (kg/kg) distribution

Contamination conversion factors (kg/kg ^ ppm)

Day	COj	NHj
1	785000	415
2	78500O	705
3	785000	1225
4	785000	2097
5	785000	3342
6	785000	4867
7	785000	7139
H	785000	9054
9	785000	11163
10	785000	1 1384

Cage occupied zone average relative humidity (%) distribution

Volume II -144

Ventilation Design Handbook on Animal Research Facilities Using Static Microisolators

Casename	Case 44		Description
Supply	Supply Discharge	Supply Discharge Exhaust	Exhaust	Exhaust
Configuration	Temperature (°C)	RH	Configuration	Temperature (°C)	RH
Radial (rot 90°)	18.8	61%	Ceiling	22	50%
Change	Rack	Rack	Number of	Total mass of	Room
Station	Orientation	Density	Mice in Room	Mice in Room	Pressurisation
ON	On wall	Single	1050	21000 gr	neg lOOcfm

Room

ACH

15

Cage

Condition

Top On

Cage Occupied Zone

Analysis Results

Volume II — Section IV - Whole Room Simulation Database

Page II - 145

Histogram Distributions

Cage occupied zone average temperature (°C) distribution

Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors

(kg/kg ^ ppm)

Day	CO2	NHj
1	785000	417
2	785000	795
3	785000	1225
4	785000	2233
5	785000	3696
6	785000	5454
7	785000	8140
8	785000	10167
9	785000	12285
10	785000	1 1384

Cage occupied zone average relative humidity (%) distribution

Volume II -146

Ventilation Design Handbook on Animal Research Facilities Using Static Microisolators

Casename Case 45

Description

Supply	Supply Discharge	Supply Discharge	Exhaust	Exhaust	Exhaust
Configuration	Temperature (°C)	RH	Configuration	Temperature (°C)	RH
Radial	18.8	61%	Ceiling	22	50%
Change	Rack	Rack	Number of	Total mass of	Room
Station	Orientation	Density	Mice in Room	Mice in Room	Pressurisation
ON	On wall	Single	1050	21000 gr	neg 50cfm

Room

ACH

15

Cage

Condition

Top On

Tag^CccupieJTonr

Analysis Results

Volume II - Section IV - Whole Room Simulation Database

Page 11-147

Histogram Distributions

Cage occupied zone average temperature (°C) distribution

Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors

(kg/kg ^ ppm)

Day	CO2	NHj
.'I.	785000	415
2	785000	795
3	785000	1225
4	785000	2127
5	785000	3419
6	785000	4995
7	785000	7358
8	785000	9298
9	785000	11408
10	785000	11384

Cage occupied zone average relative humidity (%) distribution

Volume II -148

Ventilation Design Handbook on Animal Research Facilities Using Static Microisolators

Casename Case 46

Description

Supply	Supply Discharge	Supply Discharge	Exhaust	Exhaust	Exhaust
Conflguration	Temperature (°C)	RH	Configuration	Temperature (°C)	RH
Radial	18.8	61%	Ceiling	22	50%
Change	Rack	Rack	Number of	Total mass of	Room
Station	Orientation	Density	Mice in Room	Mice in Room	Pressurisation
ON	On wall	Single	1050	21000 gr	neutral

Room

ACH

15

Cage

Condition

Top On

TagTCccupIeS^onT

Analysis Results

Volume II - Section IV - Whole Room Simulation Database

Page II - 149

Histogram Distributions

Cage occupied zone average temperature (°C) distribution

Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors

(kg/kg ^ ppm)

Day	COj	NHj
1	785000	416
2	785000	795
3	785000	1225
4	785000	2160
5	785000	3506
6	785000	5139
7	785000	7604
8	785000	9571
9	785000	11684
10	785000	11384

Cage occupied zone average relative humidity (%) distribution

Volume II -150

Ventilation Design Handbook on Animal Research Facilities Using Static Microisolators

Casename Case 47

Description

Supply	Supply Discharge	Supply Discharge	Exhaust	Exhaust	Exhaust
Configuration	Temperature (°C)	RH	Configuration	Temperature (°C)	RH
Radial	18.8	61%	Ceiling	22	50%
Change	Rack	Rack	Number of	Total mass of	Room
Station	Orientation	Density	Mice in Room	Mice in Room	Pressurisation
ON	On wall	Single	1050	21000 gr	pos 50cfm

Room

Acn

15

Cage

Condition

Top On

Tag^ncajpie^^onT

Analysis Results

Volume II - Section IV - Whole Room Simulation Database

Page II - 151

Histogram Distributions

Cage occupied zone average temperature (°C) distribution

Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors

(kg/kg -> ppm)

Day	CO2	NHj
1	785000	415
2	785000	795
3	785000	1225
4	785000	2146
5	785000	3470
6	785000	5079
7	785000	7501
8	785000	9456
9	785000	11568
10	785000	11384

Cage occupied zone average relative humidity (%) distribution

Volume II -152

Ventilation Design Handbook on Animal Research Facilities Using Static Microisolators

Casename Case 48

Description

Supply

Configuration

Radial

Supply Discharge Temperature (°C) 18.8

Supply Discharge RH

61%

Exhaust

Configuration

Ceiling

Exhaust Exhaust

Temperature (°C) RH 22 50%

Change Rack Rack

Station Orientation Density

ON (swapped with radDn wall Single

Number of Mice in Room

1050

Total mass of Mice in Room

21000 gr

Room

Pressurisation

neg lOOcfm

Room

ACH

15

Cage

Condition

Top On

Tage^ccupIeTZon^

Analysis Results

Volume II - Section IV - Whole Room Simulation Database

Page II- 153

Histogram Distributions

Cage occupied zone average temperature (°C) distribution

Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors

(kg/kg ^ ppm)

Day	CO2	NHj
1	785000	417
2	785000	795
3	785000	1225
4	785000	2237
5	785000	3704
6	785000	5468
7	785000	8165
8	785000	10195
9	785000	12313
10	785000	11384

Cage occupied zone average relative humidity (%) distribution

Volume II -154

Ventilation Design Handbook on Animal Research Facilities Using Static Microisolators

Casename Case 49

Description

Supply

Configuration

Radial

Supply Discharge	Supply Discharge	Exhaust	Exhaust	Exhaust
Temperature (°C)	RH	Configuration	Temperature (°C)	RH
18.8	61%	Ceiling	22	50%

Change Rack Rack

Station Orientation Density

ON (swapped with racOn wall Single

Number of Mice in Room

1050

Total mass of Mice in Room

21000 gr

Room

Pressurisation

neg lOOcfm

Room

ACH

15

Cage

Condition

Top On

Tage^ccupieTTonT

Analysis Results

Volume II - Section IV - Whole Room Simulation Database

Page II - 155

Histogram Distributions

Cage occupied zone average temperature (°C) distribution

Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors

(kg/kg ppm)

Day	CO2	NHj
1	785000	416
2	785000	795
3	785000	1225
4	785000	2156
5	785000	3496
6	785000	5123
7	785000	7576
8	785000	9540
9	785000	11652
10	785000	1 1384

Cage occupied zone average relative humidity (%) distribution

Volume II -156

Ventilation Design Handbook on Animal Research Facilities Using Static Microisolators

Casename Case 50

Description

Supply

Configuration

Radial

Supply Discharge	Supply Discharge	Exhaust	Exhaust	Exhaust
Temperature (°C)	RH	Configuration	Temperature (°C)	RH
18.8	61%	Ceiling	22	50%

Change Rack Rack

Station Orientation Density

ON (swapped with racDn wall Single

Number of Mice in Room

1050

Total mass of Mice in Room

21000 gr

Room

Pressurisation

neg lOOcfm

Room

ACH

15

Cage

Condition

Top On

TagTCmipieS^onr'

Analysis Results

Volume n - Section IV - Whole Room Simulation Database

Page II -157

Histogram Distributions

Cage occupied zone average temperature (°C) distribution

Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors

(kg/kg ^ ppm)

Day	CO2	NHj
1	785000	413
2	785000	795
3	785000	1225
4	785000	2019
5	785000	3139
6	785000	4531
7	785000	6567
8	785000	8418
9	785000	10521
10	785000	1 1384

Cage occupied zone average relative humidity (%) distribution

Volume II -158

Ventilation Design Handbook on Animal Research Facilities Using Static Microisolators

Casename Case 51

Description

Supply

Configuration

Radial

Supply Discharge	Supply Discharge	Exhaust	Exhaust	Exhaust
Temperature (°C)	RH	Configuration	Temperature (°C)	RH
18.8	61%	Ceiling	22	50%

Change Rack Rack

Station Orientation Density

ON (swapped with racDn wall Single

Number of Mice in Room 1050

Total mass of Mice in Room

21000 gr

Room

Pressurisation

neg lOOcfm

Room

ACH

15

Cage

Condition

Top On

Cage Occupied Zone

Analysis Results

Volume II - Section IV - Whole Room Simulation Database

Page II- 159

Histogram Distributions

Cage occupied zone average temperature (°C) distribution

Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors

(kg/kg ^ ppm)

Day	CO2	NHj
1	785000	416
2	785000	795
3	785000	1225
4	785000	2166
5	785000	3521
6	785000	5163
7	785000	7645
8	785000	9617
9	785000	1 1730
10	785000	1 1384

Cage occupied zone average relative humidity (%) distribution

Volume II -160

Ventilation Design Handbook on Animal Research Facilities Using Static Microisolators

Casename Case 52

Description

Supply

Configuration

Radial

Supply Discharge Supply Discharge Temperature (°C) RH 18.8 61%

Exhaust

Configuration

Ceiling

Exhaust Exhaust

Temperature (°C) RH 22 50%

Change Rack Rack

Station Orientation Density

ON (swapped with radDn wall Single

Number of Mice in Room

1050

Total mass of Mice in Room

21000 gr

Room

Pressurisation

neg lOOcfm

Room

ACH

15

Cage

Condition

Top On

Tage^ccupTeS^onT

Analysis Results

Volume II - Section IV - Whole Room Simulation Database

Page II - 161

Histogram Distributions

Cage occupied zone average temperature (°C) distribution

Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors

(kg/kg ^ ppm)

Day	CO2	NHj
1	785000	415
2	785000	795
3	785000	1225
4	785000	2118
5	785000	3396
6	785000	4956
7	785000	7292
8	785000	9224
9	785000	11334
10	785000	11384

Cage occupied zone average relative humidity (%) distribution

Volume II -162

Ventilation Design Handbook on Animal Research Facilities Using Static Microisolators

Casename Case 53

Description

Supply	Supply Discharge	Supply Discharge	Exhaust	Exhaust	Exhaust
Configuration	Temperature (°C)	RH	Configuration	Temperature (°C)	RH
Radial	18.8	61%	Ceiling	22	50%
Change	Rack	Rack	Number of	Total mass of	Room
Station	Orientation	Density	Mice in Room	Mice in Room	Pressurisation
ON alt design	On wall	Single	1050	21000 gr	neg lOOcfm

Room

ACH

15

Cage

Condition

Top On

T^ag^Cccupie^TonT

Analysis Results

	Temperature	COj	— RB
	(ppm)
Mean	19.77 67.59	58	57.45%
S.D.	0.16 0.:58	24
Max.	21.55 70.80	168
Room Breathing Zone NH3 (ppm)
Day	I	2	3	4	5	6	7	S	9	IQ
Mean	p o	0.06	o.oO	0.1 8	0.32	0.40	0.74	(T9T	TUt	1.10
Max.		0.17	UlE	0.53	0.93	1.40	2.14	TE2	JM	3.18

Volume II - Section IV - Whole Room Simulation Database

Page 11-163

Histogram Distributions

Cage occupied zone average temperature (°C) distribution

Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors

(kg/kg -> ppm)

Day	CO2	NHj
1	785000	422
2	785000	795
3	785000	1225
4	785000	2490
5	785000	4362
6	785000	6558
7	785000	10023
8	785000	12260
9	785000	14396
10	785000	11384

Cage occupied zone average relative humidity (%) distribution

Volume II -164

Ventilation Design Handbook on Animal Research Facilities Using Static Microisolators

Casename Case 54

Description

Supply	Supply Discharge	Supply Discharge	Exhaust	Exhaust	Exhaust
Configuration	Temperature (°C)	RH	Configuration	Temperature (°C)	RH
Slot	18,8	61%	Ceiling	22	50%
Change	Rack	Rack	Number of	Total mass of	Room
Station	Orientation	Density	Mice in Room	Mice in Room	Pressurisation
ON alt design	On wall	Single	1050	21000 gr	neg lOOcfm

Room

ACH

15

Cage

Condition

Top On

Cage OccupieJTon^

Analysis Results

Volume II - Section IV - Whole Room Simulation Database

Page II - 165

Histogram Distributions

Cage occupied zone average temperature (°C) distribution

Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors

(kg/kg ^ ppm)

Day	CO2	NHj
1	785000	417
2	785000	795
3	785000	1225
4	785000	2233
5	785000	3694
6	785000	5451
7	785000	8136
8	785000	10163
9	785000	12281
10	785000	11384

Cage occupied zone average relative humidity (%) distribution

Volume II -166

Ventilation Design Handbook on Animal Research Facilities Using Static Microisolators

Casename Case 55

Description

Supply	Supply Discharge	Supply Discharge	Exhaust	Exhaust	Exhaust
Configuration	Temperature (°C)	RH	Configuration	Temperature (°C)	RH
Low Ind	18,8	61%	Ceiling	22	50%
Change	Rack	Rack	Number of	Total mass of	Room
Station	Orientation	Density	Mice in Room	Mice in Room	Pressurisation
ON alt design	On wall	Single	1050	21000 gr	neg lOOcfm

Room

ACH

15

Cage

Condition

Top On

TagTCccupIeTTonT'

Analysis Results

Volume II - Section IV - Whole Room Simulation Database

Page II - 167

Histogram Distributions

Cage occupied zone average temperature (°C) distribution

Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors

(kg/kg -> ppm)

Day	CO2	NHj
1	785000	420
2	785000	795
3	785000	1225
4	785000	2395
5	785000	4115
6	785000	6148
7	785000	9324
8	785000	1 1483
9	785000	13612
10	785000	11384

Cage occupied zone average relative humidity (%) distribution

0

Volume II -168

Ventilation Design Handbook on Animal Research Facilities Using Static Microisolators

Casename Case 56

Description

Supply	Supply Discharge	Supply Discharge	Exhaust	Exhaust	Exhaust
Configuration	Temperature (°C)	RH	Configuration	Temperature (°C)	RH
Radial	18.8	61%	High	22	50%
Change	Rack	Rack	Number of	Total mass of	Room
Station	Orientation	Density	Mice in Room	Mice in Room	Pressurisation
ON alt design	On wall	Single	1050	21000 gr	neg lOOcfm

Room

ACH

15

Cage

Condition

Top On

Tag^HmlpIe^^on^

Analysis Results

Volume II - Section IV - Whole Room Simulation Database

Page 11-169

Histogram Distributions

Cage occupied zone average temperature (°C) distribution

Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors

(kg/kg ^ ppm)

Day	o p	NHj
1	785000	414
2	785000	795
3	785000	1225
4	785000	2084
5	785000	3309
6	785000	4812
7	785000	7047
8	785000	8952
9	785000	11059
10	785000	11384

Cage occupied zone average relative humidity (%) distribution

0

Volume II -170

Ventilation Design Handbook on Animal Research Facilities Using Static Microisolators

Casename Case 57

Description

Supply	Supply Discharge	Supply Discharge	Exhaust	Exhaust	Exhaust
Configuration	Temperature (°C)	RH	Configuration	Temperature (°C)	RH
Radial	18,8	61%	Low	22	50%
Change	Rack	Rack	Number of	Total mass of	Room
Station	Orientation	Density	Mice in Room	Mice in Room	Pressurisation
ON alt design	On wall	Single	1050	21000 gr	neg lOOcfm

Room

ACH

15

Cage

Condition

Top On

Cage Occupied Zone

Analysis Results

Volume II - Section IV - Whole Room Simulation Database

Page II - 171

Histogram Distributions

Cage occupied zone average temperature (°C) distribution

Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors

(kg/kg ppm)

Day	CO2	NHj
I	785000	408
2	785000	795
3	785000	1225
4	785000	1766
5	785000	2483
6	785000	3443
7	785000	4712
8	785000	6356
9	785000	8442
10	785000	1 1384

Cage occupied zone average relative humidity (%) distribution

0

Volume 11-172

Ventilation Design Handbook on Animal Research Facilities Using Static Microisolators

Casename Case 58

Description

Supply	Supply Discharge	Supply Discharge	Exhaust	Exhaust	Exhaust
Configuration	Temperature (°C)	RH	Configuration	Temperature (°C)	RH
Slot	18.8	61%	High	22	50%
Change	Rack	Rack	Number of	Total mass of	Room
Station	Orientation	Density	Mice in Room	Mice in Room	Pressurisation
ON alt design	On wall	Single	1050	21000 gr	neg lOOcfm

Room

ACH

15

Cage

Condition

Top On

Tag^HcaipIe^Ton^

Analysis Results

Volume II - Section IV - Whole Room Simulation Database

Page II - 173

Histogram Distributions

Cage occupied zone average temperature (°C) distribution

Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors

(kg/kg ^ ppm)

Day	CO2	NHj
1	785000	409
2	785000	795
3	785000	1225
4	785000	1806
5	785000	2586
6	785000	3614
7	785000	5004
8	785000	6681
9	785000	8770
10	785000	1 1384

Cage occupied zone average relative humidity (%) distribution

Volume II -174

Ventilation Design Handbook on Animal Research Facilities Using Static Microisolators

Casename Case 59

Description

Supply	Supply Discharge	Supply Discharge	Exh^st	Exhaust	Exhaust
Configuration	Temperature (°C)	RH	Configuration	Temperature (°C)	RH
Slot	18.8	61%	Low	22	50%
Change	Rack	Rack	Number of	Total mass of	Room
Station	Orientation	Density	Mice in Room	Mice in Room	Pressurisation
ON alt design	On wall	Single	1050	21000 gr	neg lOOcfm

Room

ACH

15

Cage

Condition

Top On

tSginnicnipieTZJ

upie

Analysis Results

Volume II - Section IV - Whole Room Simulation Database

Page II - 175

Histogram Distributions

Cage occupied zone average temperature (°C) distribution

Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors

(kg/kg -> ppm)

Day	CO2	NHj
1	785000	419
2	785000	795
3	785000	1225
4	785000	2318
5	785000	3916
6	785000	5819
7	785000	8763
S	785000	10860
9	785000	12984
10	785000	11384

Cage occupied zone average relative humidity (%) distribution

Volume II -176

Ventilation Design Handbook on Animal Research Facilities Using Static Microisolators

Casename Case 60

Description

Supply

Configuration

Low Ind

Supply Discharge Temperature (°C) 18.8

Supply Discharge RH

61%

Exhaust

Configuration

High

Exhaust Exhaust

Temperature (°C) RH

22 50%

Change

Station

ON alt design

Rack

Orientation

On wall

Rack

Density

Single

Number of Mice in Room

1050

Total mass of Mice in Room

21000 gr

Room

Pressurisation

neg lOOcfm

Room

ACH

15

Cage

Condition

Top On

TagrCccupien^onr"

Analysis Results

Volume II - Section IV - Whole Room Simulation Database

Page 11-177

Histogram Distributions

Cage occupied zone average temperature (°C) distribution

Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors

(kg/kg ^ ppm)

Day	CO2	NHj
1	785000	422
2	785000	795
3	785000	1225
4	785000	2520
5	785000	4439
6	785000	6686
7	785000	10242
8	785000	12503
9	785000	14641
10	785000	11384

Cage occupied zone average relative humidity (%) distribution

Volume II -178

Ventilation Design Handbook on Animal Research Facilities Using Static Microisolators

Casename Case 61

Description

Supply	Supply Discharge	Supply Discharge	Exhaust	Exhaust	Exhaust
Configuration	Temperature (°C)	RH	Configuration	Temperature (°C)	RH
Low Ind	18.8	61%	Low	22	50%
Change	Rack	Rack	Number of	Total mass of	Room
Station	Orientation	Density	Mice in Room	Mice in Room	Pressurisation
ON alt design	On wall	Single	1050	21000 gr	neg lOOcfm

Room

ACH

15

Cage

Condition

Top On

TagrT5ccup!eT2Ion^

Analysis Results

Volume II - Section IV - Whole Room Simulation Database

Page II - 179

Histogram Distributions

Cage occupied zone average temperature (°C) distribution

Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors

(kg/kg ppm)

Day	CO2	NH3
1	785000	409
2	785000	795
3	785000	1225
4	785000	1818
5	785000	2618
6	785000	3668
7	785000	5095
8	785000	6782
9	785000	8872
10	785000	11384

Cage occupied zone average relative humidity (%) distribution

Volume II -180

Ventilation Design Handbook on Animal Research Facilities Using Static Microisolators

Casename Case 62

Description

Supply	Supply Discharge	Supply Discharge	Exhaust	Exhaust	Exhaust
Configuration	Temperature (°C)	RH	Configuration	Temperature (°C)	RH
Low Ind	6.6	89%	Low	22	50%
Change	Rack	Rack	Number of	Total mass of	Room
Station	Orientation	Density	Mice in Room	Mice in Room	Pressurisation
ON	Perp	Double	2100	42000 gr	neg lOOcfm

Room

ACH

5

Cage

Condition

Top On

Cage Occuple^^on^

Analysis Results

Volume II - Section IV - Whole Room Simulation Database

Page II - 181

Histogram Distributions

Cage occupied zone average temperature (°C) distribution

Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors

(kg/kg ^ ppm)

Day	CO2	Nllj
1	785000	415
2	785000	795
3	785000	1225
4	785000	2129
5	785000	3425
6	785000	5004
7	785000	7374
8	785000	9315
9	785000	1 1426
10	785000	1 1384

Cage occupied zone average relative humidity (%) distribution

Volume II -182

Ventilation Design Handbook on Animal Research Facilities Using Static Microisolators

Casename Case 63

Description

Supply	Supply Discharge	Supply Discharge	Exhaust	Exhaust	Exhaust
Conflguration	Temperature (°C)	RH	Configuration	Temperature (°C)	RH
Low Ind	14.8	79%	Low	22	50%
Change	Rack	Rack	Number of	Total mass of	Room
Station	Orientation	Density	Mice in Room	Mice in Room	Pressurisation
ON	Perp	Double	2100	42000 gr	neg lOOcfm

Analysis Results

TTag^Cccupie^^onT

Volume II - Section IV - Whole Room Simulation Database

Page II - 183

Histogram Distributions

Cage occupied zone average temperature (°C) distribution

Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors

(kg/kg ppm)

Day	CO^	NHj
1	785000	415
2	785000	795
3	785000	1225
4	785000	2129
5	785000	3425
6	785000	5004
7	785000	7374
8	785000	9315
9	785000	11426
10	785000	1 1384

Cage occupied zone average relative humidity (%) distribution

Volume II -184

Ventilation Design Handbook on Animal Research Facilities Using Static Microisolators

Casename Case 64

Description

Supply	Supply Discharge	Supply Discharge	Exhaust	Exhaust	Exhaust
Configuration	Temperature (°C)	RH	Configuration	Temperature (°C)	RH
Low Ind	18.9	60%	Low	22	50%
Change	Rack	Rack	Number of	Total mass of	Room
Station	Orientation	Density	Mice in Room	Mice in Room	Pressurisation
ON	Perp	Double	2100	42000 gr	neg lOOcfm

Analysis Results

TagTCccupieTTon?

Volume II - Section IV - Whole Room Simulation Database

Page II - 185

Histogram Distributions

Cage occupied zone average temperature (°C) distribution

Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors

(kg/kg ppm)

Day	O o	NHj
1	785000	408
2	785000	795
3	785000	1225
4	785000	1766
5	785000	2483
6	785000	3443
7	785000	4712
8	785000	6356
9	785000	8442
10	785000	1 1384

Cage occupied zone average relative humidity (%) distribution

0

Volume II -186

Ventilation Design Handbook on Animal Research Facilities Using Static Microisolators

Casename Case 65

Description

Supply	Supply Discharge	Supply Discharge	Exhaust	Exhaust	Exhaust
Configuration	Temperature (°C)	RH	Configuration	Temperature (°C)	RH
Low Ind	17.5	66%	Low	22	50%
Change	Rack	Rack	Number of	Total mass of	Room
Station	Orientation	Density	Mice in Room	Mice in Room	Pressurisation
ON alt design	Perp	Double	2100	42000 gr	neg lOOcfm

Room

ACH

15

Cage

Condition

Top On

Cage Occupied Zone

Analysis Results

Volume II - Section IV - Whole Room Simulation Database

Page II - 187

Histogram Distributions

Cage occupied zone average temperature (°C) distribution

Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors

(kg/kg ^ ppm)

Day	COj	NHj
1	785000	411
2	785000	795
3	785000	1225
4	785000	1921
5	785000	2886
6	785000	41 1 1
7	785000	5850
8	785000	7622
9	785000	9718
10	785000	11384

Cage occupied zone average relative humidity (%) distribution

Volume II -188

Ventilation Design Handbook on Animal Research Facilities Using Static Microisolators

Casename	Case 66		Description
Supply	Supply Discharge	Supply Discharge Exhaust	Exhaust	Exhaust
Configuration	Temperature (°C)	RH	Configuration	Temperature (°C)	RH
Low Ind	6.6	89%	Low	22	50%
Change	Rack	Rack	Number of	Total mass of	Room
Station	Orientation	Density	Mice in Room	Mice in Room	Pressurisation
ON alt design	Perp	Double	2100	42000 gr	neg lOOcfm

Room

ACH

5

Cage

Condition

Top On

T^geTJccupiedZoiie

Analysis Results

Volume II - Section IV - Whole Room Simulation Database

Page II - 189

Histogram Distributions

Cage occupied zone average temperature (°C) distribution

Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors

(kg/kg ^ ppm)

Day	o p	NHj
1	785000	408
2	785000	795
3	785000	1225
4	785000	1766
5	785000	2483
6	785000	3443
7	785000	4712
8	785000	6356
9	785000	8442
10	785000	11384

Cage occupied zone average relative humidity (%) distribution

Volume II -190

Ventilation Design Handbook on Animal Research Facilities Using Static Microisolators

Casename Case 67

Description

Supply	Supply Discharge	Supply Discharge	Exhaust	Exhaust	Exhaust
Configuration	Temperature (°C)	RH	Configuration	Temperature (°C)	RH
Low Ind	14.8	79%	Low	22	50%
Change	Rack	Rack	Number of	Total mass of	Room
Station	Orientation	Density	Mice in Room	Mice in Room	Pressurisation
ON alt design	Perp	Double	2100	42000 gr	neg lOOcfm

Room

ACH

10

Cage

Condition

Top On

TagTCccupien^onT

Analysis Results

	Temperature		— RH —
T ^	(ppm)
Mean	20.19 68.35	201	57.23%
S.D.	0.52 0.93	90
Max.	21.51 70.72	623
Room Breathing Zone NH3 (ppm)
Day	I	2	3	4	5	6	7	8	9	10
Mean	0.11	TIU	on	0.57	on	1.37	2M	2.55	3.10	3.42'
Max.	0.33	0.63	(W7	1.75		4.25	6.32	7n	0:00	ion

Volume II - Section IV - Whole Room Simulation Database

Page II - 191

Histogram Distributions

Cage occupied zone average temperature (°C) distribution

Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors

(kg/kg ^ ppm)

Day	CO2	NHj
1	785000	417
2	785000	795
3	785000	1225
4	785000	2209
5	785000	3633
6	785000	5350
7	785000	7963
8	785000	9970
9	785000	12087
10	785000	1 1384

Cage occupied zone average relative humidity (%) distribution

Volume II -192

Ventilation Design Handbook on Animal Research Facilities Using Static Microisolators

Casename Case 68

Description

Supply	Supply Discharge	Supply Discharge	Exhaust	Exhaust	Exhaust
Configuration	Temperature (°C)	RH	Conflguration	Temperature (°C)	RH
Low Ind	18.9	60%	Low	22	50%
Change	Rack	Rack	Number of	Total mass of	Room
Station	Orientation	Density	Mice in Room	Mice in Room	Pressurisation
ON alt design	Perp	Double	2100	42000 gr	neg lOOcfm

Room

ACH

20

Cage

Condition

Top On

Analysis Results

Cage Occupied Zone

Volume II - Section IV - Whole Room Simulation Database

Page 11-193

Histogram Distributions

Cage occupied zone average temperature (°C) distribution

Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors

(kg/kg ppm)

Day	CO2	NHj
1	785000	408
2	785000	795
3	785000	1225
4	785000	1766
5	785000	2483
6	785000	3443
7	785000	4712
8	785000	6356
9	785000	8442
10	785000	11384

Cage occupied zone average relative humidity (%) distribution

Volume II -194

Ventilation Design Handbook on Animal Research Facilities Using Static Microisolators

Casename Case 69

Description

Supply	Supply Discharge	Supply Discharge	Exhaust	Exhaust	Exhaust
Conflguration	Temperature (°C)	RH	Conflguration	Temperature (°C)	RH
Radial	18.8	61%	High x4 / Low x4	22	50%
Change	Rack	Rack	Number of	Total mass of	Room
Station	Orientation	Density	Mice in Room	Mice in Room	Pressurisation
ON	On wall	Single	1050	21000 gr	neg lOOcfm

Room

ACH

15

Cage

Condition

Top On

Tage^iTcupIe^^onT

Analysis Results

Volume II - Section IV - Whole Room Simulation Database

Page 11-195

Histogram Distributions

Cage occupied zone average temperature (°C) distribution

Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors

(kg/kg -> ppm)

Day	CO2	NHj
1	785000	408
2	785000	795
3	785000	1225
4	785000	1766
5	785000	2483
e	785000	3443
7	785000	4712
8	785000	6356
9	785000	8442
10	785000	11384

Cage occupied zone average relative humidity (%) distribution

Volume II -196

Ventilation Design Handbook on Animal Research Facilities Using Static Microisolators

Casename Case 70

Description

Supply	Supply Discharge	Supply Discharge	Exhaust	Exhaust	Exhaust
Configuration	Temperature (°C)	RH	Configuration	Temperature (°C)	RH
Slot	18.8	61%	High x4 / Low x4	22	50%
Change	Rack	Rack	Number of	Total mass of	Room
Station	Orientation	Density	Mice in Room	Mice in Room	Pressurisation
ON	On wall	Single	1050	21000 gr	neg lOOcfm

Room

ACH

15

Cage

Condition

Top On

TagTCceuple^Ton^

Analysis Results

Volume II - Section IV - Whole Room Simulation Database

Page 11-197

Histogram Distributions

Cage occupied zone average temperature (°C) distribution

Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors

(kg/kg -> ppm)

Day	CO2	NHj
,.t	785000	410
2	785000	795
3	785000	1225
4	785000	1839
5	785000	2673
C	785000	3758
7	785000	5250
8	785000	6954
9	785000	9045
10	785000	11384

Cage occupied zone average relative humidity (%) distribution

Volume II -198

Ventilation Design Handbook on Animal Research Facilities Using Static Microisolators

Casename Case 71

Description

Supply	Supply Discharge	Supply Discharge	Exhaust	Exhaust	Exhaust
Configuration	Temperature (°C)	RH	Configuration	Temperature (°C)	RH
Low Ind	18.8	61%	High x4 / Low x4	22	50%
Change	Rack	Rack	Number of	Total mass of	Room
Station	Orientation	Density	Mice in Room	Mice in Room	Pressurisation
ON	On wall	Single	1050	21000 gr	neg lOOcfm

Analysis Results

T^ag^CccupIe^^onT

Volume II - Section IV - Whole Room Simulation Database

Page 11-199

Histogram Distributions

Cage occupied zone average temperature (°C) distribution

Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors

(kg/kg ^ ppm)

Day	COj	NHj
1	785000	409
2	785000	795
3	785000	1225
4	785000	1821
5	785000	2626
6	785000	3680
7	785000	5116
8	785000	6805
9	785000	8894
10	785000	11384

Cage occupied zone average relative humidity (%) distribution

Volume II -200

Ventilation Design Handbook on Animal Research Facilities Using Static Microisolators

Casename Case 72

Description

Supply	Supply Discharge	Supply Discharge	Exhaust	Exhaust	Exhaust
Configuration	Temperature (°C)	RH	Configuration	Temperature (°C)	RH
Radial	18.8	61%	High x4 / Low x2	22	50%
Change	Rack	Rack	Number of	Total mass of	Room
Station	Orientation	Density	Mice in Room	Mice in Room	Pressurisation
ON	On wall	Single	1050	21000 gr	neg lOOcfm

Analysis Results

Tag^dmlple^^onr

Volume II - Section IV - Whole Room Simulation Database

Page II - 201

Histogram Distributions

Cage occupied zone average temperature (°C) distribution

Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors

(kg/kg ppm)

Day	CO2	NH3
1	785000	418
2	785000	795
3	785000	1225
4	785000	2303
5	785000	3876
6	785000	5752
7	785000	8650
8	785000	10734
9	785000	12856
10	785000	11384

Cage occupied zone average relative humidity (%) distribution

Volume II -202

Ventilation Design Handbook on Animal Research Facilities Using Static Microisolators

Casename Case 73

Description

Supply	Supply Discharge	Supply Discharge	Exhaust	Exhaust	Exhaust
Configuration	Temperature (°C)	RH	Configuration	Temperature (°C)	RH
Slot	18.8	61%	High x4 / Low x2	22	50%
Change	Rack	Rack	Number of	Total mass of	Room
Station	Orientation	Density	Mice in Room	Mice in Room	Pressurisation
ON	On wall	Single	1050	21000 gr	neg lOOcfm

Room

ACH

15

Cage

Condition

Top On

Tage^ccupIeTZonr"

Analysis Results

Volume II - Section IV - Whole Room Simulation Database

Page II - 203

Histogram Distributions

Cage occupied zone average temperature (°C) distribution

Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors

(kg/kg ^ ppm)

Day	CO2	NHj
1	785000	412
2	785000	795
3	785000	1225
4	785000	1947
5	785000	2952
6	785000	4221
7	785000	6038
8	785000	7831
9	785000	9929
10	785000	1 1384

Cage occupied zone average relative humidity (%) distribution

Volume II -204

Design Handbook on Animal Research Facilities Using Static Microisolators

Casename	Case 74		Description
Supply	Supply Discharge	Supply Discharge Exhaust	Exhaust	Exhaust
Configuration	Temperature (°C)	RH	Configuration	Temperature (°C)	RH
Low Ind	18.8	61%	High x4 / Low x2	22	50%
Change	Rack	Rack	Number of	Total mass of	Room
Station	Orientation	Density	Mice in Room	Mice in Room	Pressurisation
ON	On wall	Single	1050	21000 gr	neg lOOcfm

Room

ACH

15

Cage

Condition

Top On

Tag^nccupiet^onT

Analysis Results

Volume II - Section IV - Whole Room Simulation Database

Page II - 205

Histogram Distributions

Cage occupied zone average temperature (°C) distribution

Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors

(kg/kg ^ ppm)

Day	CO,	NHj
1	785(M)0	41 1
2	785000	795
3	7^5000	1225
4	785000	IW
5	785000	2800
6	785000	3969
7	785000	5609
a	785000	7353
9	785000	9447
10	7ii5000	11384

Cage occupied zone average relative humidity (%) distribution

Volume II -206

Design Handbook on Animal Research Facilities Using Static Microisolators

Casename Case 75

Description

Supply

Configuration

Radial

Supply Discharge Temperature (°C) 18.8

Supply Discharge RH

61%

Exhaust

Configuration

Ceiling/Low 50/50

Exhaust

Temperature (°C) 22

Exhaust

RH

50%

Change

Station

ON alt design

Rack

Orientation

On wall

Rack

Density

Single

Number of Mice in Room 1050

Total mass of Mice in Room

21000 gr

Room

Pressurisation

neg lOOcfm

Room

ACH

15

Cage

Condition

Top On

TTag^Cccupie^TonT

Analysis Results

	Temperature	— UJT~	— RH —
^ cp	(ppm)
Mean	2 1 .09 69.96	48	52.74%
S.b.	(HI ^	22
Max.	22.37 72.26	247
Room Breathing Zone NH3 (ppm)
Day	I	2	3		5	6	7	g	9	10
Mean	0.03	0.05	0T)7	(TTl	— m —	— OT“	0715	0.57	urn
Max.	0,1 3	0.25	0.30	OTt	— n5s~	1.57	Tn	193	3.60

Volume II - Section IV - Whole Room Simulation Database

Page II - 207

Histogram Distributions

Cage occupied zone average temperature (°C) distribution

Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors

(kg/kg ^ ppm)

Day	COj	NHj
1	7t^5O0O	415
2	785000	795
3	785000	1225
4	785000	2127
5	785000	3420
6	78500O	4996
1	785000	7360
8	785000
9	785000	TTTRJ
10	7S5000	11384

Cage occupied zone average relative humidity (%) distribution

Volume II -208

Design Handbook on Animal Research Facilities Using Static Microisolators

Casename	Case 76		Description
Supply	Supply Discharge	Supply Discharge Exhaust	Exhaust	Exhaust
Configuration	Temperature (°C)	RH	Configuration	Temperature (°C)	RH
Radial	18.8	61%	Ceiling	22	50%
Change	Rack	Rack	Number of	Total mass of	Room
Station	Orientation	Density	Mice in Room	Mice in Room	Pressurisation
ON	On wall	Single	1050	21000 gr	neg lOOcfm

Analysis Results

iL'age i?5ccupled Zone

Volume II - Section IV - Whole Room Simulation Database

Page II - 209

Histogram Distributions

Cage occupied zone average temperature (°C) distribution

Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors

(kg/kg ^ ppm)

Day	COj	NHj
1	7S5O0O	414
2	785000	795
3	785000	i:>55
4	785000	2049
5	785o0o	3218
	785000	4662
7	785000	6791
H	785000	8667
9	785000	10772
10	7!)5000	11384

Cage occupied zone average relative humidity (%) distribution

Volume II -210

Design Handbook on Animal Research Facilities Using Static Microisolators

Casename Case 77

Description

Supply	Supply Discharge	Supply Discharge	Exhaust	Exhaust	Exhaust
Configuration	Temperature (°C)	RH	Configuration	Temperature (°C)	RH
Slot	18.8	61%	Ceiling	22	50%
Change	Rack	Rack	Number of	Total mass of	Room
Station	Orientation	Density	Mice in Room	Mice in Room	Pressurisation
ON	On wall	Single	1050	21000 gr	neg lOOcfm

Analysis Results

TageTJcajpieT^nT

Volume II - Section IV - Whole Room Simulation Database

Page 11-211

Histogram Distributions

Cage occupied zone average temperature (°C) distribution

Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors

(kg/kg ^ ppm)

Day	CO,	NHj
1	78500O	414
2	785000	795
3	785000	1225
4	785000	>060
5	785000	5247
6	785000	4710
7	78$O00	6872
8	785000	8758
9	785000	10864
10	785000	11384

Cage occupied zone average relative humidity (%) distribution

Volume II -212

Design Handbook on Animal Research Facilities Using Static Microisolators

Casename Case 78

Description

Supply	Supply Discharge	Supply Discharge	Exhaust	Exhaust	Exhaust
Configuration	Temperature (°C)	RH	ConTiguration	Temperature (°C)	RH
Low Ind	18.8	61%	Ceiling	22	50%
Change	Rack	Rack	Number of	Total mass of	Room
Station	Orientation	Density	Mice in Room	Mice in Room	Pressurisation
ON	On wall	Single	1050	21000 gr	neg lOOcfm

Room

ACH

15

Cage

Condition

Top On

Cage OccupTeTZonT

Analysis Results

Volume II - Section IV - Whole Room Simulation Database

Page 11-213

Histogram Distributions

Cage occupied zone average temperature (°C) distribution

Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors

(kg/kg ^ ppm)

Day	COj	NHj
1	785000	415
2	785O00	795
3	785000	1225
4	785000	2109
5	785000	~i7rr
6	785000	4919
7	785000	7229
8	785000	9154
	785000	11263
10	785O00	11584

Cage occupied zone average relative humidity (%) distribution

Volume II -214

Design Handbook on Animal Research Facilities Using Static Microisolators

Casename Case 79

Description

Supply	Supply Discharge	Supply Discharge	Exhaust	Exhaust	Exhaust
Configuration	Temperature (°C)	RH	Configuration	Temperature (°C)	RH
Radial	18.8	61%	2 Door exhausts	22	50%
Change	Rack	Rack	Number of	Total mass of	Room
Station	Orientation	Density	Mice in Room	Mice in Room	Pressurisation
ON	On wall	Single	1050	21000 gr	neg lOOcfm

Room

ACH

15

Cage

Condition

Top On

TagTC^upied itone

Analysis Results

Volume II - Section IV - Whole Room Simulation Database

Page II - 215

Histogram Distributions

Cage occupied zone average temperature (°C) distribution

Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors

(kg/kg -> ppm)

Day	COj	NH3
1	7i)5()00	408
1	785000	795
3	785000	1225
4	785000	1766
5	785000	2485
6	785000
7	785000	4712
8	7850O0
9	785000	8445
10	7S5000	11384

Cage occupied zone average relative humidity (%) distribution

Volume II -216

Design Handbook on Animal Research Facilities Using Static Microisolators

Casename Case 80

Description

Supply	Supply Discharge	Supply Discharge	Exhaust	Exhaust	Exhaust
Configuration	Temperature (°C)	RH	Configuration	Temperature (°C)	RH
Slot	18.8	61%	2 Door exhausts	22	50%
Change	Rack	Rack	Number of	Total mass of	Room
Station	Orientation	Density	Mice in Room	Mice in Room	Pressurisation
ON	On wall	Single	1050	21000 gr	neg lOOcfm

Room

ACH

15

Cage

Condition

Top On

^agTCccupIen^o

upie

Analysis Results

Volume II - Section IV - Whole Room Simulation Database

Page II - 217

Histogram Distributions

Cage occupied zone average temperature (°C) distribution

Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors

(kg/kg ^ ppm)

Day	COj	NH3
1	785OO0	40i}
2	785000	795
3	785000	1225
4	785000	1766
5	785000	2483
6	785O00	3443
7	785000	47 1 7
H	785000	6356
9	785000	8442
10	785000	11384

Cage occupied zone average relative humidity (%) distribution

Volume II -218

Design Handbook on Animal Research Facilities Using Static Microisolators

Casename Case 81

Description

Supply	Supply Discharge	Supply Discharge	Exhaust	Exhaust	Exhaust
Configuration	Temperature (°C)	RH	Configuration	Temperature (°C)	RH
Low Ind	18.8	61%	2 Door exhausts	22	50%
Change	Rack	Rack	Number of	Total mass of	Room
Station	Orientation	Density	Mice in Room	Mice in Room	Pressurisation
ON	On wall	Single	1050	21000 gr	neg lOOcfm

Room

ACH

15

Cage

Condition

Top On

^ag^CccupIeTZone

upieo

Analysis Results

Volume II - Section IV - Whole Room Simulation Database

Page II - 219

Histogram Distributions

Cage occupied zone average temperature (°C) distribution

Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors

(kg/kg ^ ppm)

Day	COj	NHj
1	785000	408
2	785000	795
3	7J15000	1225
4	785000	1766
5	785000	2483
6	785000	3443
7	785000	4712
	785000	6356
“9~	785000	8442
~nr	71)5000	11584

Cage occupied zone average relative humidity (%) distribution

Volume II -220

Design Handbook on Animal Research Facilities Using Static Microisolators

Casename Case 82

Description

Supply	Supply Discharge	Supply Discharge	Exhaust	Exhaust	Exhaust
Configuration	Temperature (°C)	RII	Configuration	Temperature (°C)	RH
Radial	18.8	61%	2 Door exhausts	22	50%
Change	Rack	Rack	Number of	Total mass of	Room
Station	Orientation	Density	Mice in Room	Mice in Room	Pressurisation
ON	On wall	Single	1050	21000 gr	neg lOOcfm

Room

ACH

15

Cage

Condition

Top On

Tage^ccupi^r^onr

Volume II - Section IV - Whole Room Simulation Database

Page II - 221

Histogram Distributions

Cage occupied zone average temperature (°C) distribution

Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors

(kg/kg ^ ppm)

Day	COj	NHj
1	785000	410
2	785000	795
3	785000	\ns
4	785000	1848
5	78?00O	2605
6	785000	3795
7	785000	5313
»	785000	7024
	785000	9115
10	785000	11384

Cage occupied zone average relative humidity (%) distribution

Volume II -222

Design Handbook on Animal Research Facilities Using Static Microisolators

Casename Case 83

Description

Supply	Supply Discharge	Supply Discharge	Exhaust	Exhaust	Exhaust
Configuration	Temperature (°C)	RH	Conflguration	Temperature (°C)	RH
Slot	17.5	66%	2 Door exhausts	22	50%
Change	Rack	Rack	Number of	Total mass of	Room
Station	Orientation	Density	Mice in Room	Mice in Room	Pressurisation
ON	Perp	Double	2100	42000 gr	neg lOOcfm

Room

ACH

15

Cage

Condition

Top On

Cage Occupie^^nT

Analysis Results

Volume II - Section IV - Whole Room Simulation Database

Page II - 223

Histogram Distributions

Cage occupied zone average temperature (°C) distribution

Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors

(kg/kg ^ ppm)

Day	CO,	NH,
1	785000	418
1	785000	795
5	785000	1225
4	785000	7303
5	785000	3876
6	785000	5752
7	785000	8650
	785000	10734
9		12856
10	785000	11384

Cage occupied zone average relative humidity (%) distribution

Volume II -224

Design Handbook on Animal Research Facilities Using Static Microisolators

Casename Case 84

Description

Supply	Supply Discharge	Supply Discharge	Exhaust	Exhaust	Exhaust
Configuration	Temperature (°C)	RH	Configuration	Temperature (°C)	RH
Low Ind	17.5	66%	2 Door exhausts	22	50%
Change	Rack	Rack	Number of	Total mass of	Room
Station	Orientation	Density	Mice in Room	Mice in Room	Pressurisation
ON	Perp	Double	2100	42000 gr	neg lOOcfm

Room

ACH

15

Cage

Condition

Top On

7ag^5ccupie3T^on^

Analysis Results

Volume II - Section IV - Whole Room Simulation Database

Page II - 225

Histogram Distributions

Cage occupied zone average temperature (°C) distribution

Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors

(kg/kg ^ ppm)

Day	CO,	NHj
1	7$50O()	408
2	7i)5()O0	795
3	785000	1225
4	785000	1766
5		748?
6	7t)5()O0	344?
7	7t)5000	4712
a	7i)5000	6356
9	785000	8442
10	"7^'0()U	11384

Cage occupied zone average relative humidity (%) distribution

0

Volume II -226

Design Handbook on Animal Research Facilities Using Static Microisolators

Cusename Case 85

Description

Supply	Supply Discharge	Supply Discharge	Exhaust	Exhaust	Exhaust
Conflguration	Temperature (°C)	RH	Conflguration	Temperature (°C)	RH
Radial	17,5	66%	Low	22	50%
Change	Rack	Rack	Number of	Total mass of	Room
Station	Orientation	Density	Mice in Room	Mice in Room	Pressurisation
ON alt design	Perp	Double	2100	42000 gr	neg lOOcfm

Room

ACH

15

Cage

Condition

Top On

TagTCccupietTronT'

Analysis Results

Volume II - Section IV - Whole Room Simulation Database

Page II - 227

Histogram Distributions

Cage occupied zone average temperature (°C) distribution

Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors

(kg/kg ^ ppm)

Day	COj	NHj
1	785000	4ll
2	7^5000	795
3	7i<5000	1225
4	785000	1935
5	785000	2922
6	7^5000	4171
7	785000	5950
8	785000	7736
	785000	9833
10	785000	11384

Cage occupied zone average relative humidity (%) distribution

Volume II -228

Design Handbook on Animal Research Facilities Using Static Microisolators

Casename Case 86

Description

Supply	Supply Discharge	Supply Discharge	Exhaust	Exhaust	Exhaust
Conflguration	Temperature (°C)	RH	Configuration	Temperature (°C)	RH
Slot	17.5	66%	Low	22	50%
Change	Rack	Rack	Number of	Total mass of	Room
Station	Orientation	Density	Mice in Room	Mice in Room	Pressurisation
ON alt design	Perp	Double	2100	42000 gr	neg lOOcfm

Room

ACH

15

Cage

Condition

Top On

Cage Dccupie^^n^

Analysis Results

Volume II - Section IV - Whole Room Simulation Database

Page II - 229

Histogram Distributions

Cage occupied zone average temperature (°C) distribution

Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors

(kg/kg ^ ppm)

Day	CO2	NHj
1	7Ji5000	4l0
2	785000	705
3	785000	12^5
4	785000	1859
5	78500O	2776
6	785000	3846
7	785000	5400
»	785000	7121
	785000	9213
10	785000	11384

Cage occupied zone average relative humidity (%) distribution

0

Volume II -230

Design Handbook on Animal Research Facilities Using Static Microisolators

Casename Case 87

Description

Supply	Supply Diseharge	Supply Discharge	Exhaust	Exhaust	Exhaust
Configuration	Temperature (°C)	RH	Conflguration	Temperature (°C)	RH
Radial	19.2	59%	Low	22	50%
Change	Rack	Rack	Number of	Total mass of	Room
Station	Orientation	Density	Mice in Room	Mice in Room	Pressurisation
ON	On wall	Reduced	700	14000 gr	neg lOOcfm

Room

ACH

15

Cage

Condition

Top On

^gTCccupIeTTonT

Analysis Results

Volume II - Section IV - Whole Room Simulation Database

Page ll - 231

Histogram Distributions

Cage occupied zone average temperature (°C) distribution

Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors

(kg/kg -> ppm)

Day	CO^	NHj
1	7S5O0O	408
2	785000	795
3	785000	1225
4	785000	1766
5	785000	2483
6	785000	3443
7	785000	4712
»	7j)5000	6356
	785000	8442
10	785000	11384

Cage occupied zone average relative humidity (%) distribution

Volume II -232

Design Handbook on Animal Research Facilities Using Static Microisolators

Casename Case 88

Description

Supply	Supply Discharge	Supply Discharge	Exhaust	Exhaust	Exhaust
Configuration	Temperature (°C)	RH	Configuration	Temperature (°C)	RH
Slot	19.2	59%	Low	22	50%
Change	Rack	Rack	Number of	Total mass of	Room
Station	Orientation	Density	Mice in Room	Mice in Room	Pressurisation
ON	On wall	Reduced	700	14000 gr	neg lOOcfm

Analysis Results

7"ag^0mipieT^onr

Volume II - Section IV - Whole Room Simulation Database

Page II - 233

Histogram Distributions

Cage occupied zone average temperature (°C) distribution

Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors

(kg/kg ^ ppm)

Day	C02	NHj
1	785O0O	40S
2	785000	795
3	785000	1225
4	785O00	1766
5	785000	2483
6	785O0O
7	785000	4712
a	785000	6356
9	785000	8442
10	785000	TUM

Cage occupied zone average relative humidity (%) distribution

Volume II -234

Design Handbook on Animal Research Facilities Using Static Microisolators

Casename Case 89

Description

Supply	Supply Discharge	Supply Discharge	Exhaust	Exhaust	Exhaust
Conflguration	Temperature (°C)	RH	Configuration	Temperature (°C)	RH
Low Ind	19.2	59%	Low	22	50%
Change	Rack	Rack	Number of	Total mass of	Room
Station	Orientation	Density	Mice in Room	Mice in Room	Pressurisation
ON	On wall	Reduced	700	14000 gr	neg lOOcfm

Room

ACH

15

Cage

Condition

Top On

7ageT5ccupie^Ton^

Analysis Results

Volume II - Section IV - Whole Room Simulation Database

Page II - 235

Histogram Distributions

Cage occupied zone average temperature (°C) distribution

Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors

(kg/kg ^ ppm)

Day	CO,	NHj
I	785000	408
2	785000	795
3	785000	1225
4	785000	1766
5	785000	248$
6	785000	3443
7	785000	4712
a	785000	6356
	785000	~mr
10	785000	11384

Cage occupied zone average relative humidity (%) distribution

0

Volume II -236

Design Handbook on Animal Research Facilities Using Static Microisolators

Casename	Case 90		Description
Supply	Supply Discharge	Supply Discharge Exhaust	Exhaust	Exhaust
Configuration	Temperature (°C)	RH	Configuration	Temperature (°C)	RH
Radial	18.8	61%	Ceiling	22	50%
Change	Rack	Rack	Number of	Total mass of	Room
Station	Orientation	Density	Mice in Room	Mice in Room	Pressurisation
ON	Perp all 5 on 1 wall	Single	1050	21000 gr	neg lOOcfm

Room

ACH

15

Cage

Condition

Top On

c’age Occupl^^one

Analysis Results

Volume II - Section IV - Whole Room Simulation Database

Page II - 237

Histogram Distributions

Cage occupied zone average temperature (°C) distribution

Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors

(kg/kg ^ ppm)

Day	CO^	NHj
1	785000	412
1	785OO0	795
3	785000	1225
4	785000	1982
5	785000	3043
6	7850O0	4371
7	785000	6295
it	785000	8116
9	785000	10216
10	785000	11384

Cage occupied zone average relative humidity (%) distribution

0

Volume II -238

Design Handbook on Animal Research Facilities Using Static Microisolators

Casename	Case 91		Description
Supply	Supply Discharge	Supply Discharge Exhaust	Exhaust	Exhaust
Configuration	Temperature (°C)	RH	Configuration	Temperature (°C)	RH
Slot	18.8	61%	Ceiling	22	50%
Change	Rack	Rack	Number of	Total mass of	Room
Station	Orientation	Density	Mice in Room	Mice in Room	Pressurisation
ON	Perp all 5 on 1 wall	Single	1050	21000 gr	neg lOOcfm

Room

ACH

15

Cage

Condition

Top On

Tag^CcoIpIeT^on^

Analysis Results

Volume II - Section IV - Whole Room Simulation Database

Page II - 239

Histogram Distributions

Cage occupied zone average temperature (°C) distribution

Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors

(kg/kg ^ ppm)

Day	CO,	NHj
1	785000	412
2	785000	795
3	785000	1225
4	785000	1987
5	785000	3056
6	785000	4394
7	785000	6333
8	785000	8158
	785000	10259
10	785000	11384

Cage occupied zone average relative humidity (%) distribution

Volume II -240

Design Handbook on Animal Research Facilities Using Static Microisolators

Casename Case 92

Description

Supply

Configuration

Low Ind

Supply Discharge Temperature (°C) 18.8

Supply Discharge Exhaust RH Configuration

61% Ceiling

Exhaust Exhaust

Temperature (°C) RH

22 50%

Change

Station

ON

Rack	Rack	Number of	Total mass of	Room
Orientation	Density	Mice in Room	Mice in Room	Pressurisation
Perp all 5 on 1 wall	Single	1050	21000 gr	neg lOOcfm

Room

ACH

15

Cage

Condition

Top On

Cage Occupied Zone

Analysis Results

Volume II - Section IV - Whole Room Simulation Database

Page II - 241

Histogram Distributions

Cage occupied zone average temperature (°C) distribution

Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors

(kg/kg ^ ppm)

Day	COj	NHj
1	785000	4l 1
2	785O0O	795
3	785000	1225
4	785000
5	7k5o0O	2916
6	785000	4161
7	785000	5936
8	7^5000	7716
	785000	9814
10	785000	11384

Cage occupied zone average relative humidity (%) distribution

0

Volume II -242

Design Handbook on Animal Research Facilities Using Static Microisolators

Casename	Case 93		Description
Supply	Supply Discharge	Supply Discharge Exhaust	Exhaust	Exhaust
Configuration	Temperature (°C)	RH	Configuration	Temperature (°C)	RH
Radial	17.5	66%	Ceiling	22	50%
Change	Rack	Rack	Number of	Total mass of	Room
Station	Orientation	Density	Mice in Room	Mice in Room	Pressurisation
ON	Perp all 5 on 1 wall	Double	2100	42000 gr	neg lOOcfm

Room

ACH

15

Cage

Condition

Top On

Tagrnccupie3Tonr"

Analysis Results

Volume II - Section IV - Whole Room Simulation Database

Page II - 243

Histogram Distributions

Cage occupied zone average temperature (°C) distribution

Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors

(kg/kg ^ ppm)

E>ay	COj	NHj
i	7850OO	415
2	785000	795
3	785000	1225
4	785000	:)i4i
5	785000
6	785000	5058
7	785000	7466
K	785000	94l8
9	785000	11529
10	785000	TUP’

Cage occupied zone average relative humidity (%) distribution

Volume II -244

Design Handbook on Animal Research Facilities Using Static Microisolators

Casename	Case 94		Description
Supply	Supply Discharge	Supply Discharge Exhaust	Exhaust	Exhaust
Configuration	Temperature (°C)	RH	Configuration	Temperature (°C)	RH
Slot	17.5	66%	Ceiling	22	50%
Change	Rack	Rack	Number of	Total mass of	Room
Station	Orientation	Density	Mice in Room	Mice in Room	Pressurisation
ON	Perp all 5 on 1 wall	Double	2100	42000 gr	neg lOOcfm

Room

ACH

15

Cage

Condition

Top On

TagTCcajp^enZonT

Analysis Results

Volume II - Section IV - Whole Room Simulation Database

Page II - 245

Histogram Distributions

Cage occupied zone average temperature (°C) distribution

Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors

(kg/kg ^ ppm)

Day	COj	NHj
1	785000	414
2	7^5000	795
3	785000	1225
4	785000	OT80
5	7850OO	5200
6	785000	4705
7	785000	7017
B	785000	8910
9	785000	11026
10	78 5 DOT	11584

Cage occupied zone average relative humidity (%) distribution

Volume II -246

Design Handbook on Animal Research Facilities Using Static Microisolators

Casename	Case 95		Description
Supply	Supply Discharge	Supply Discharge Exhaust	Exhaust	Exhaust
Configuration	Temperature (°C)	RH	Configuration	Temperature (°C)	RH
Low Ind	17.5	66%	Ceiling	22	50%
Change	Rack	Rack	Number of	Total mass of	Room
Station	Orientation	Density	Mice in Room	Mice in Room	Pressurisation
ON	Perp all 5 on 1 wall	Double	2100	42000 gr	neg lOOcfm

Analysis Results

Tag^nccupie^TonT

Volume II - Section IV - Whole Room Simulation Database

Page II - 247

Histogram Distributions

Cage occupied zone average temperature (°C) distribution

Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors

(kg/kg ^ ppm)

Day	COj	NHj
1	785000	414
2	78500d	795
J	785000	1225
4	785OO0	2054
s	785000	3231
6	785000	4684
7	785000	6827
K	785000	"StUT
9	785000	10814
10	785000	TOM

Cage occupied zone average relative humidity (%) distribution

Volume II -248

Design Handbook on Animal Research Facilities Using Static Microisolators

Casename Case 96

Description

Supply	Supply Discharge	Supply Discharge	Exhaust	Exhaust	Exhaust
Configuration	Temperature (°C)	RH	Configuration	Temperature (°C)	RH
Radial	17.5	66%	Ceiling	22	50%
Change	Rack	Rack	Number of	Total mass of	Room
Station	Orientation	Density	Mice in Room	Mice in Room	Pressurisation
ON alt design	Perp all 5 on 1 wall	Double	2100	42000 gr	neg lOOcfm

Analysis Results

Tage^coipIe3T5onr

Volume II - Section IV - Whole Room Simulation Database

Page II - 249

Histogram Distributions

Cage occupied zone average temperature (°C) distribution

Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors

(kg/kg ^ ppm)

Day	COj	NHj
1	785000	417
2	785000	795
3	785000	1225
4	785000	2210
5	785000	3636
6	785000	5355
7	785000	7972
8	785000
9	785O00	12097
10	785000	11384

Cage occupied zone average relative humidity (%) distribution

0

Volume II -250

Design Handbook on Animal Research Facilities Using Static Microisolators

Casename	Case 97		Description
Supply	Supply Discharge	Supply Discharge Exhaust	Exhaust	Exhaust
Configuration	Temperature (°C)	RH	Configuration	Temperature (°C)	RH
Slot	17.5	66%	Ceiling	22	50%
Change	Raek	Rack	Number of	Total mass of	Room
Station	Orientation	Density	Mice in Room	Mice in Room	Pressurisation
ON alt design	Perp all 5 on 1 wall	Double	2100	42000 gr	neg lOOcfm

Room

ACH

15

Cage

Condition

Top On

Cage Occupied Zone

Analysis Results

Volume II - Section IV - Whole Room Simulation Database

Page II - 251

Histogram Distributions

Cage occupied zone average temperature (°C) distribution

Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors

(kg/kg ^ ppm)

Day	CO,	NHj
1		414
2	785000	795
5	785000	1225
4	785000	2065
5	785000	3259
6	785000	4730
7	785O00	6907
8	785000	8796
9	785000	10902
10	785000	1 1384

Cage occupied zone average relative humidity (%) distribution

Volume II -252

Design Handbook on Animal Research Facilities Using Static Microisolators

Casename	Case 98		Description
Supply	Supply Discharge	Supply Discharge Exhaust	Exhaust	Exhaust
Configuration	Temperature (°C)	RH	Configuration	Temperature (°C)	RH
Low ind	17.5	66%	Ceiling	22	50%
Change	Rack	Rack	Number of	Total mass of	Room
Station	Orientation	Density	Mice in Room	Mice in Room	Pressurisation
ON alt design	Perp all 5 on 1 wall	Double	2100	42000 gr	neg lOOcfm

Room

ACH

15

Cage

Condition

Top On

Tag^nccupie^Tone

Analysis Results

Volume II - Section IV - Whole Room Simulation Database

Page II - 253

Histogram Distributions

Cage occupied zone average temperature (°C) distribution

Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors

(kg/kg -> ppm)

Day	COj	NHj
1	7i)5000	417
2	785000	795
5	785000	\ns
4	785000
5	785000	3636
6	785000	5355
7	785000	7971
a	785(K)0	9979
9	785000	12096
10	785006	11384

Cage occupied zone average relative humidity (%) distribution

0

Volume II -254

Design Handbook on Animal Research Facilities Using Static Microisolators

Casename	Case 99		Description
Supply	Supply Discharge	Supply Discharge Exhaust	Exhaust	Exhaust
Configuration	Temperature (°C)	RH	Conflguration	Temperature (°C)	RH
Radial	22	49%	Ceiling	26	50%
Change	Rack	Rack	Number of	Total mass of	Room
Station	Orientation	Density	Mice in Room	Mice in Room	Pressurisation
ON	On wall	Single	1050	21000 gr	neg lOOcfm

Room

ACH

15

Cage

Condition

Top On

Tag^Tccupiet^onT

Analysis Results

Volume II - Section IV - Whole Room Simulation Database

Page II - 255

Histogram Distributions

Cage occupied zone average temperature (°C) distribution

Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors

(kg/kg ^ ppm)

Day	COj	NHj
1	785000	408
2	785000	795
3	7850OO	1^55
4	785000	1766
5	785000	2483
6	785000	3443
7	785000	4712
s	785000	6356
9	785000	8442
10	785000	11384

Cage occupied zone average relative humidity (%) distribution

Volume 11-256

Design Handbook on Animal Research Facilities Using Static Microisolators

Casename Case 100

Description

Supply

Configuration

Radial

Supply Discharge Supply Discharge Temperature (°C) RH

22 49%

Exhaust

Configuration

Low

Exhaust Exhaust

Temperature (°C) RH

25 50%

Change Rack Rack

Station Orientation Density

ON On wall Single

Number of Mice in Room

1050

Total mass of Mice in Room

21000 gr

Room

Pressurisation

neg lOOcfm

Room

ACH

15

Cage

Condition

Top On

7agn5mIpie^^onr

Analysis Results

Volume II - Section IV - Whole Room Simulation Database

Page !I - 257

Histogram Distributions

Cage occupied zone average temperature (°C) distribution

Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors

(kg/kg ^ ppm)

Day	COj	NHj
1	785000
2	785000	795
3	7^5000	1225
4	785000	TfW
5	TSW	2483
6	785000
7	785000	4712
8	785000	~5JW
9	785000	8442
10	785000	11384

Cage occupied zone average relative humidity (%) distribution

Volume II -258

Design Handbook on Animal Research Facilities Using Static Microisolators

Casename Case 101

Description

Supply	Supply Discharge	Supply Discharge	Exhaust	Exhaust	Exhaust
Configuration	Temperature (°C)	RH	Configuration	Temperature (°C)	RH
Low Ind	22	49%	Low	25	50%
Change	Rack	Rack	Number of	Total mass of	Room
Station	Orientation	Density	Mice in Room	Mice in Room	Pressurisation
ON alt design	Perp	Double	1050	21000 gr	neg lOOcfm

Room

ACH

5

Cage

Condition

Top On

Cage O ccuple^^r

Analysis Results

	Temperature	— usr~	— m —
		T	(ppm)
Mean	26,24	79.22	284	39.78%
S.D.	1.52	2.74	00
Max.	28.22	82.79	793

Room Breathing Zone NH3 (ppm)

15iy	1	2	3	3	5	5	7	g	5	m
Mean	0.15	(120	on	on	0:00	1.25	1.71	230	2.06	TOO
Max.	on	0.80	1.24	1.78	2.51	00	TIE	6.42		11.15

Volume II - Section IV - Whole Room Simulation Database

Page I! - 259

Histogram Distributions

Cage occupied zone average temperature (°C) distribution

Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors

(kg/kg ^ ppm)

Day	CO,	NHj
1	7850O0	408
2	7«500()	795
3	785000	1225
4	7850O0	1766
5	785000	TtST
6	785000	3443
7	785000	4712
8	785000	6356
	785000	8447
10	785000	11384

Cage occupied zone average relative humidity (%) distribution

• LIBRARY

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Amazing Research. Amazing Heip.

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