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GIFT   OF 


AGRIC. 
LIBRAH 


MINI 


TECHNICAL    AND    SCIENTIFIC    SERVIC 


Bulletin  IVo. 


(ENT.  i  ..OGICAL    SE 


THE  LIFE  HISTORY 


THE  TIM 


nu   '  )TTON  HARV 


P.T,    I 


ORGANIZATION  OF  THE  MINISTRY  OF  AGRICULTURE,  CAIRO,  EGYPT. 


Minister      

Under-Secretary  of  State. 
Consulting- Agriculturist  . 


inspector-General 


W.  Cartwright,  M.A., 

T.  L.  Smith. 

J.  Wright. 

R.  W.  Brigstocke. 


H.E.  Ahmed  Helmy  Pasha. 

J.  Haines. 

Q.  C.  Dudgeon,  F.E.S.,  Vice-President  of  the  International 
Association  of  Tropical  Agriculture,  Correspondent  of  the 
International  Institute  of  Agriculture,  Rome,  sto. 

A.  T.  McKHIop. 


PROVINCIAL     INSPECTORS. 


J.  D.  Shepherd. 
H.  M.  Heaid. 
C.  A.  Williams. 
T.  E.  Bayne-Jardine. 


Ibrahim  Bey  Fahmy. 
Daoud  Eff.  Fat'hi. 
Mohammed  Eff.  Kamel. 
Mohammed  Eff.  Osman. 


Director 


Director 
Entomologist!! 


ADMINISTRATIVE    AND    STATISTICAL    SERVICE. 

„     ...       Sadik  Bey  Henein. 


ENTOMOLOGICAL    SECTION. 

L.  H.  Gough,  Ph.D.,  F.E.S. 
(    Gilbert  Storey,  B.A.,  F.E.S. 
,     E.  W.  Adair,  B.A.,  F.E.S. 


HORTICULTURAL    SECTION. 


Director      

Assistant- Director 
Superintendent  ... 


T.  W.  Brown. 

F.  C.  Walsingham. 

C.  S.  Crouch. 


BOTANICAL    AND    MYCOLOG1CAL    SECTION,    GIZA. 

{Cotton.  Breeding  F'.trm  «t  Gha.) 

Botanist     B.  G.  C.  Bolland,  B.A. 

CHEMICAL    LABORATORY. 

Chemist F.  Hughes,   F.C.S. 

COTTON    SEED    DISTRIBUTION. 

Director  ...  A.  S.  Morton. 

BUREAU    OF    COMMERCIAL    INTELLIGENCE. 

Directed  by  the  Consulting-Agriculturist. 


Director     

Pathologist 

Veterinary  Inspectors 


VETERINARY    SERVICE. 

W.  Littlewood,  M.R.C.V.S.  Egyptian  Government  Delegate, 

International  Quarantine  Board,  Egypt. 
.       F.  E.  Mason,  M.R.C.V.S. 
H.  Cooper,  M.R.C.V.S. 
H.  Mason,  M.R.C.V.S. 
E.  de  R.  Gordon,  M.R.C.V.S. 


Principal 


F.  W.  Trydell,  M.R.C.V.S. 

SCHOOL    OF    VETERINARY     MEDICINE. 

D.  S,  Rabagliati,  B.Sc.,  Agrlo.  (Edin.),  M.R.C.V.S. 

HIGHER    SCHOOL    OF    AGRICULTURE,    GIZA. 


Director  ......... 

Lecturer,  Chein.  and  Agrlc.Chem.  ... 

Lecturer  in  Physics  ...........  . 

Lecturer  in  Botany  ............ 

Lecturer  in  Agriculture  ......... 


Ernest  Shearer,   M.A.,  B.Sc. 
G.  Burns,  B.Sc. 

J.  W.  McPherson,  B.A.,  A.R.C.Sc. 
J.  R.  Davidson,  M.  A.,  B.Sc. 
Alphonse  M.  Greiss. 


MLMSTKY   OF  AGRICULTURE,   EGYPT. 


TECHNICAL     AND     SCIENTIFIC     SERVICE. 


Bulletin  !\o.  4. 


(ENTOMOIjOGIGAL    SEGTIOX.j 

t 

THE  LIFE  HISTORY  OF  GKLEGHIA  0088YPIBLLA 

FROM  THE  TIME  OF  THE  COTTON  HARVEST  TO  THE  TIME 

OF  COTTON  SOWING, 

BY 

LEWIS  GOUGH,  Pii.I).,  F.E.S..  i:n  .. 

% 

DIBBOTOH  «>r  THE  KNTOMOUMJIOAI  SHCTION. 


ur   I'l-'uillnij  VH   Man-It  39,  I'Jlti.) 


CAIRO. 

(jrOVKUNMKNT 

to  l>f  obtiiinod,  ••ithi-r  din-otly   or  through  any  l.uoksfllor. 

II.MM    III*    UMVI:I:XMIAT    I'IIBSS*,    iiula'j.    ;md    I'rotn    th«    S A l,K- K"< > M . 

(Mil    I  ni'iili  i    I'tilaijr,  Shnrhi  Qai-r  el  Aini. 

1910. 
PRICE  P.T.  I. 


MINISTRY   OF  AGRICULTURE,  EGYPT. 


\o.    4. 


(KNTOMOIA )( MGAL     SECTION.) 


THE    LIFE    HISTORY    OF    GELECHIA    GOSSYPIELLA 

FROM  THE  TIME   OF  THE  COTTON  HARVEST 

TO  THE  TIME  OF  COTTON  SOWING. 

The  whole  problem  of  the.  control  of  Gdechia  gossypidla  Saund. 
is  closely  bound  up  with  the  solution  of  the  question  where  and  how 
Gdechia  passes  the  late  autumn  and  winter. 

As  is  well  known  in  the  summer,  from  June  onwards,  Gelechia 
gossypidla  passes  through  many  generations,  each  of  which  lasts 
only  a  few  weeks.  Each  successive  generation  is  immensely  more 
numerous  and  more  important  in  the  damage  it  does,  than  the  preceding 
one.  Towards  late  autumn,  however,  we  find  a  change;  with  the 
advent  of  colder  weather  the  larvae  no  longer  pupate,  but  hibernate 
unchanged  when  full  fed,  pupation  taking  place,  on  the  advent  of 
warm  weather,  next  spring. 

The  object  of  this  paper  is  to  show  what  happens  with  the  seed 
worm  between  the  time  of  the  first  picking  and  the  time  of  sowing, 
this  being  the  period  during  which  the  pest  can  be  controlled  most 
effectively. 

It  is  so  well  known  to  cultivators  and  ginners  that  it  is  not 
necessary  to  insist  on  the  enormous  increase  of  the  pest  between  the 
first  and  second  pickings.  As  a  general  rule  the  first  picking  is 
affected  only  to  a  slight  degree,  whilst  the  second  is  seriously  damaged, 
and  if  the  harvesting  of  a  third  picking  were  attempted,  it  would  in 
most  places  be  found  to  be  worthless.  Four  or  five  years  ago  three 
pickings  appear  to  have  been  the  rule,  but  this  year,  owing  .to  Gdechia 
attacks,  few  cultivators  in  Lower  Egypt  obtained  a  third  picking. 

The  increase  of  the  pest  between  the  first  and  second  pickings 
can  be  most  easily  gauged  by  examination  of  commercial  cotton  s 


—  2  - 

Thus  it  was  found  that  in  a  first  picking  sample  only  eighty  seeds  per 
thousand  had  been  damaged  by  the  seed  worm  as  against  377  seeds 
per  thousand  damaged  by  this  insect  in  a  second  picking  sample, 
showing  an  increase  of  207  per  thousand  in  damaged  seed  during  the 
interval  of  approximately  six  weeks  that  separates  the  first  and  second 
pickings. 

The  extent  of  the  increase  in  numbers  at  this  period  is,  however, 
not  so  interesting  from  the  point  of  view  of  pest  control,  as  is  the 
fact  that  during  the  harvest  season  (i.e.  the  period  including  the  first 
and  second  pickings)  the  life-cycle  of  Gelechia  becomes  modified,  and 
that  it  is  soon  after  the  first  picking  that  the  full  fed  seed  worm  inter- 
calates a  period  of  hibernation  as  larva  before  pupating. 

Examining  first  picking  seed  in  January  a  certain  number  of 
Gelechia  larvae  are  found  hibernating,  together  with  a  number  of  dead 
worms.  These  hibernating  larvae  were  not  necessarily  full  grown  at 
the  time  of  picking,  or  of  ginning;  safely  hidden  in  the  "  double" 
seeds  they  can  have  finished  feeding  after  both  of  these  operations 
were  completed.  In  the  case  of  the  dead  worms  there  is  of  course 
nothing  to  show  when  or  how  they  died.  Taking  the  number  of 
living  hibernating  worms,  found  in  January  in  first  picking  seed 
and  comparing  them  with  the  number  of  damaged  seeds,  six  living 
and  thirteen  dead  worms  were  found  to  eighty  damaged  seeds,  or 
seventy-five  living  worms  in  one  thousand  damaged  seeds. 

For  second  picking  seed  the'  numbers  of  hibernating  worms 
present  in  January  are  much  greater.  The  sample  we  examined 
contained  377  damaged  seeds  in  every  thousand  seeds.  In  this  case 
118  living  and  fifty-six  dead  worms  were  found  in  the  377  damaged 
seeds,  or  313  living  worms  in  every  thousand  damaged  seeds. 

The  proportion  of  living  worms  found  in  January  in  one  thousand 
seeds  (uninjured  and  damaged)  in  the  sample  examined  consequently 
works  out  at  six  per  thousand  in  the  case  of  the  first  picking  and  118 
per  thousand  in  the  case  of  the  second  picking. 

The  question  immediately  arises,  what  has  happened  to  reduce 
the  proportion  of  living  worms  to  damaged  seeds  so  considerably 
in  the  first  picking  seed  as  compared  to  the  second  picking  seed. 

A  solution  to  this  problem  is  offered  by  the  observation  that 
very  large  quantities  of  larvae  leave  the  seed  and  pupate  on  the  sacks, 
especially  in  first  picking  seed.  The  moths  from  these  pupae  emerge 


—  3  — 

during  the  winter,  and  apparently  do  not  wander  away  from  the 
,sacks  to  any  great  extent.  During  December,  January,  and  February, 
it  is  always  possible  to  find  immense  quantities  of  dead  moths  in  the 
accumulation  of  dust  and  dirt  that  collect  on  and  between  the  sacks 
in  a  seed  store.  For  example,  scrapings  off  sacks  containing  first 
picking  seed  taken  in  February  at  Kafr  el  Zayat  were  found  to  contain 
large  quantities  of  dead  moths.  Ten  grammes  were  examined  and 
found  to  include  248  dead  moths,  forty-four  empty  pupa  cases,  and 
two  dead  larvae. 

In  second  picking  seed  at  the  same  time  of  the  year,  the  worms 
are  mostly  still  in  the  seeds,  and  have  not  emigrated  to  pupate. 

This  leads  one  inevitably  to  the  conclusion  that  the  worms  in 
the  seed  at  the  time  of  the  first  picking  do  not  to  any  great  extent 
hibernate,  but  that  they  pupate  and  emerge  during  the  course  of  the 
next  few  months.  A  further  conclusion  is  that  for  want  of  suitable 
food  plants,  or  on  account  of  the  cold  weather,  the  majority  of  these 
moths  do  not  propagate  their  species. 

The  main  danger  consequently  lies  in  the  worms  which  were 
not  full  fed  at  the  time  of  the  first  picking.  If  it  were  possible  at  the 
time  of  the  first  picking  to  destroy  all  the  cottoa  plants  and  immature 
bolls,  the  damage  to  be  anticipated  from  GeLechia  in  the  next  season 
would  be  very  slight.  Such  a  procedure  is,  however,  impossible  on 
account  of  the  great  financial  loss  involved  by  rejecting  the  second 
picking. 

A  recommendation  can,  however,  be  made  to  reserve  first  picking 
for  sowing,  and  either  to  crush  or  export  all  second  picking  seed  as 
soon  as  possible.  Alternatively  the  second  picking  seed  could  be 
treated  by  heat  or  poisonous  gas  to  kill  all  the  worms  contained, 
should  it  for  any  reason  be  desired  to  keep  it  in  Egypt.  Similar 
treatment  of  first  picking  seed  is  not  so  necessary  but  would  be 
useful. 

Not  all  the  worms  that  having  left  the  seed  wander  about  in  a 
seed  store,  pupate  on  the  sacks.  A  small  minority  is  to  be  found  in 
other  places.  Sweepings  were  taken  in  February  in  a  seed  store  at 
Kafr  el  Zayat.  639  grammes  were  examined,  which  consisted  of 
113  grammes  of  cotton  seed,  95  grammes  of  stones  or  straw,  and 
431  grammes  of  dust.  In  this  material  three  larvsS  were  found,  of 
which  two  dead.  The  living  larva  was  discovered  in  the  dust,  the 


—  4  — 

two  dead  ones  in  the  seed.     Of  one  thousand  of  the  seeds  154  had 
been  damaged  by  seed  worm. 

A  further  recommendation  is  that,  if  possible,  all  seed  cotton 
stored  in  Egypt  after  January  22  of  each  year  should  be  kept  in 
moth-proofed  stores,  and  that  cotton  seed  be  permitted  to  remain 
unprotected  until  after  planting  is  finished.  Before  this  date  it 
might  be  kept  in  open  stores  without  much  harm,  provided  they  are 
sprayed  and  swept  periodically,  as  the  majority  of  larvae  emigrating 
from  seed  and  seed  cotton  before  then  will  belong  to  generations 
bred  in  the  first  picking  cotton;  these  larvae  can  be  expected  to 
pupate  and  the  moths  to  emerge  during  winter  and  early  spring ; 
such  moths  not  finding  foodplants  to  oviposit  on  are  lost  for  the 
propagation  of  the  species,  whilst  the  hibernating  larvae  from  the 
later  seed  are  not  yet  leaving  the  seed.  Of  course  it  would  be  better 
if  all  cotton  seed  and  seed  cotton  could  be  stored  in  moth-proofed 
stores,  from  the  time  of  picking  until  ultimately  disposed  of,  but 
it  is  not  possible  to  interfere  to  so  great  an  extent  with  ordinary 
practice. 

Leaving  the  question  of  cotton  seed  and  seed  cotton  in  stores  on 
one  side,  the  much  more  important  question  arises  as  to  the  fate  of  the 
larvae  left  in  the  fields  after  the  last  picking. 

Under  the  old  boll  worm  law,  which  was  intended  to  restrict 
Earias  insulana  Boisd.,  all  cotton  sticks  have  to  be  pulled  up  by 
December  31  of  each  year.  This  is  in  order  to  reduce  the  quantity 
of  oqr  or  ratoon  cotton  which  in  the  spring  could  offer  breeding 
grounds  for  Earias  larvae.  The  law  further  ordered  all  bolls  to  be 
removed  from  the  sticks  before  December  15  in  order  to  destroy  the 
last  generation  of  Earias. 

At  first  it  was  hoped  that  vigorous  enforcement  of  this  law  would 
also  help  to  check  Gdechia  gossypiella.  It  has  without  doubt  made 
Earias  insulana  much  rarer  this  winter  (1915-1916)  than  it  used 
to  be  only  four  years  ago,  but  in  the  case  of  Gdechia  the  result  has 
not  been  appreciable. 

At  the  time  of  pulling  the  sticks  great  attention  has  been  given 
to  the  removal  and  burning  of  the  bolls,  and  the  Ministry  of  Agriculture 
has  caused  large  quantities  of  badly  cleaned  sticks  to  be  burnt.  To 
affect  Gdechia  gossypiella  this  work  ought  to  be  carried  out  at  a  much 
earlier  date  than  prescribed  by  the  Boll  Worm  Law  (Earias  Law), 


—  o  — 


and  much  more  attention  would  have  to  be  paid  to  preventing  bolls 
falling  to  the  ground  and  being  left  there  to  rot.  In  combating 
Earias,  bolls  left  on  the  ground  did  not  have  the  same  significance  as 
they  have  in  the  control  of  Gelechia. 

It  can  be  considered  as  certain  that  all  Gelechia  larvae  arriving 
at  maturity  after  the  middle  of  October  are  going  to  hibernate  before 
pupation.  As  we  have  seen  above,  it  is  the  hibernating  larvae  which 
arc  chiefly  responsible  for  carrying  on  the  pest  from  one  season  to  the 
other.  The  later  the  bolls  are  left  on  the  plants  or  in  the  field,  the 
more  certain  they  are  to  be  infested  by  Gelechia  larvae.  It  is  con- 
sequently urgently  to  be  recommended  that  the  final  destruction  of 
immature  or  worthless  bolls  should  take  place  at  the  time  of  final 
picking. 

It  has  been  almost  impossible  this  year  to  obtain  bolls  for  exam- 
ination in  January  from  cotton  sticks  or  from  cotton  plants  left 
standing  in  the  fields  or  stored  as  fuel.  That  such  bolls  are  a  favourite 
hibernating  place  for  Gelechia  larvae  is  well  known.  We  have,  however, 
been  able  to  examine  a  number  of  bolls  collected  at  Giza  in  October 
1915.  These  bolls  were  stored  in  the  insectarium  of  the  Entomological 
Section,  no  special  care  being  given  to  them.  On  February  21, 1916, 
seventy-six  bolls  were  examined  and  were  found  to  contain  five  small, 
eight  half-grown,  and  thirty-nine  full-grown  living  Gelechia  larvae, 
fourteen  dead  larvae,  and  two  empty  pupa  cases  of  Gelechia.  In  addi- 
tion to  these  three  empty  pupa  cases  of  Pyroderces  gossypiella  Wlsm., 
one  Earias  insidana  pupa,  and  one  dead  Pimpla  roburator  F.  were 
found.  Seventy-five  cotton  bolls  found  on  sticks,  used  as  supports 
fcr  peas,  were  collected  in  a  garden  near  Cairo  in  February  1916;  these 
contained  two  small,  three  half-grown,  and  one  full-grown  Gelechia 
larvae. 

The  destruction  of  the  bolls  to  be  really  effective  must  be  complete. 
Fellahm  do  not  realise  that  bolls  fallen  to  the  ground  are  as  great  a 
menace  to  the  next  year's  crop  as  bolls  left  on  the  cotton  sticks,  and 
very  much  more  serious  than  the  worms  left  in  the  seed. 

In  order  to  ascertain  what  happens  to  worms  in  bolls  lying  on 
the  ground,  two  sets  of  examinations  have  been  made.  In  one  case 
bolls  have  been  collected  from  the  ground  in  the  field  amongst  growing 
crops,  in  the  other,  seed  and  bolls  containing  worms  have  been  buried 
at  various  depths  under  growing  bersim  as  well  as  in  unwatered  land. 


The  following  monthly   results  have  been   obtained  by  the  end  of 
March  (at  the  time  of  sowing  the  1916  crop). 

Bolls  were  collected  in  fields  under  beans,  wheat,  barley,  and 
bersim,  in  the  neighbourhood  of  Shibin  el  Qanater,  on  December  26, 19 1 5, 
January  23,  February  16,  and  March  18,  1916  (see  Table  I). 

About  two  hundred  and  thirteen  bolk  were  examined  in 
December.  These  contained  twelve  small,  five  half-grown,  and  twenty 
full-grown  living  larvae,  besides  two  living  pupae  and  ten  empty  pupa 
cases  of  Gdechia. 

Three  hundred  and  fifteen  bolls  were  examined  in  January, 
containing  sixteen  small,  twenty-one  half-grown,  and  forty-three  full- 
grown  living  larvae  and  eighteen  empty  pupa  cases  of  Gdechia. 

Two  hundred  and  forty-three  bolls  were  examined  in  February, 
containing  five  small,  forty-seven  half -grown,  and  fifty-two  full-grown 
living  larvae  and  nineteen  empty  pupa  cases  of  Gdechia. 

Two  hundred  and  thirty-nine  bolls  were  examined  in  March, 
containing  one  small,  four  half-grown,  and  forty-nine  full-grown 
living  larvae,  two  living  pupae,  and  sixteen  empty J  pupa  cases  of 
Gelechia. 

It  will  be  noted  that  no  living  pupae  were  found  in  January  or 
February  and  that  they  again  occurred  in  March.  The  pupae  found 
in  March  doubtless  belonged  to  the  hibernating  generation  of  worms, 
those  found  in  December  to  the  last  maturing  generation  of  the  pre- 
vious autumn. 

Attention  is  also  drawn  to  the  steady  increase  in  the  proportion 
of  the  worms  present  in  the  bolls  during  December,  January,  and 
February,  and  the  obvious  decrease  in  March.  This  decrease  is  very 
probably  to  be  considered  in  connection  with  the  emigration  of  full 
fed  larvae  previous  to  pupation. 

It  becomes  very  obvious  from  the  study  of  Table  III  that  during 
the  winter  months  two  generations  of  Gdechia  larvae  are  found  in  the 
bolls  on  the  ground. 

The  older  of  the  two  generations,  the  hibernating  generation, 
consists  entirely  of  full-fed  worms.  These  form  about  fifty  per  cent 
of  the  total  of  worms  found  in  December,  January,  and  February ; 
and  without  doubt  compose  part  at  least  of  the,  eighty-eight  per  cent 
of  full-grown  larvae  found  in  March.  There  are,  however,  indications 
that  many  of  the  worms  of  the  hibernating  generation  must  have 
emigrated  to  pupate,  or  pupated  by  the  end  of  March, 


_     IT     

The  younger  of  the  two  generations  is  the  winter-feeding  brood. 

From  the  figures  given  in  the  table  it  is  quite  easy  to  trace  the 
slow  growth  of  the  individuals  composing  it  during  December,  January, 
and  February.  By  March  all  but  nine  per  cent  of  this  generation  were 
full  fed.  As  the  last  picking  in  the  fields  from  which  these  bolls  were 
collected  took  place  on  October  26,  and  the  cotton  sticks  were  removed 
from  the  fields  on  or  before  November  3,  1915,  there  appears  to  the 
writer  to  be  considerable  probability  that  the  eggs  from  which  the 
larvae  forming  the  winter-feeding  generation  were  derived  must  have 
been  laid  on  bolls  on  the  ground.  Should  this  be  actually  what 
happens,  bolls  left  on  the  ground  after  the  last  picking  acquire  a  still 
greater  economic  importance  than  they  already  had. 

Having  found  that  Gdechia  larvae  readily  survive  in  bolls  lying  on 
the  surface  of  the  ground  amongst  cultivated  crops,  where  the  bolls 
are  liable  to  rot  and  where  they  must  be-  submerged  every  time  the 
fields  are  irrigated,  it  is  not  surprising  to  find  that  many  of  the  larvae 
that  are  buried  at  the  time  of  preparing  the  ground  for  the  crops 
survive.  As  it  was  difficult  or  even  perhaps  impossible  to  find  such 
bolls  by  digging  in  the  fields,  experiments  in  thii  direction  were  made 
in  the  grounds  of  the  Ministry  of  Agriculture  at  Cairo. 

Seed  containing  larvae  and  "double"  seeds  were  used,  on  account 
of  the  difficulty  in  procuring  bolls  at  the  time  when  the  experiment 
was  started  (December  23,  1915).  Two  series  of  experiments  were 
made ;  small  flower  pots  were  prepared  by  having  the  drainage  hole 
being  stopped  by  plaster  of  Paris.  One  set  of  these  pots,  Series  I,  was 
filled  with  apparently  good  cotton  seed,  fifteen  full  fed  Gdechia  larvae 
placed  in  the  seed,  the  pots  were  then  closed  by  tying  muilin  over  the 
opening.  In  the  second  set,  Series  II,  the  pots  were  filled  with  infested 
seed  ("  double  "  seed)  instead  of  sound  seed  with  isolated  larvae. 

The  further  treatment  of  both  these  sets  of  pots  was  exactly  the 
same.  One  half  of  them  were  buried  in  dry  ground,  in  land  left 
unwatered  except  by  rain,  the  other  half  was  buried  in  land  on  which 
a  crop  of  bersim  was  grown. 

To  test  the  influence  of  depth  at  which  the  seed  was  buried  part 
of  the  pots  were  lowered  until  their  tops  were  five,  ten,  fifteen,  and 
twenty  centimetres  from  the  surface. 

From  these  experiments  the  depth  factor  does  not  appear  to  be 
an  important  one,  and  Gdechia  larva)  seem  to  support  being  buried 


—  8  — 

in  moist  or  dry  conditions  very  well  (see  Table  II).  On  January  23, 
when  the  first  examination  was  made  in  the  infested  seed  (Series  II), 
eighty-one  living  and  seventeen  dead  were,  found  in  the  pots  which 
had  been  buried  under  growing  bersim,  and  113  living  and  seventeen 
dead  in  the  pots  buried  in  unwatered  land. 

The  "good"  seed  (Series  I),  gave  similar  results;  here  twenty-five 
living  and  eighteen  dead  larvae  were  found  Jn  the  pots  buried  under 
bersim,  and  twenty-three  living  and  five  dead  in  the  pots  buried  in  dry 
soil.  Forty-nine  larvae  had  disappeared.  This  disappearance  is  not  to 
be  wondered  at,  as  the  gauze  covering  the  pots  had  rotted  away 
entirely. 

Two  months  were  supported  quite  as  well  as  four  weeks  inter- 
ment had  been.  On  February  23,  further  sets  of  pots  were  dug  up 
and  examined.  The  pots  containing  "  infested "  seed  (Series  II) 
buried  under  bersim  yielded  153  living  Gelechia  larvae  and  eighteen 
dead  ones,  those  buried  in  dry  soil  132  living  and  six  dead  seed  worms. 
No  pupae  were  found. 

In  the  ''good"  seed  (Series  I)  fifteen  living  and  four  dead  larvae 
were  recevered  from  pots  buried  under  bersim,  one  dead  and  twenty- 
four  living  worms  from  the  pots  buried  in  dry  land;  seventy-six 
larvae  had  disappeared. 

Although  there  were  no  double  seeds  in  the  "good"  seed  pots  at 
the  time  of  burying  (December  23,  1915)  several  were  found  when 
the  pots  were  examined,  showing  that  some  of  the  larvre  had  spun 
seeds  together  in  the  interval.  The  instinct  to  spin  up  seeds  appears 
to  have  been  stronger  in  the  case  of  worms  in  moist  surroundings. 
Attention  is  specially  drawn  to  the  fact  that  a  very  large  majority 
of  the  worms  were  found  inside  seeds  in  Series  I. 

Three  months'  burial  were  also  supported  by  a  part  of  the  worms. 
The  numbers  of  worms  found  were,  however,  distinctly  less  than  those 
recovered  in  the  earlier  examinations,  possibly  on  account  of  emigra- 
tion for  pupation. 

From  these  experiments  it  would  appear  that  the  hibernating 
or  full-fed  larvre  survive  even  if  ploughed  under  or  buried  in  seed, 
or  in  bolls,  and  that  they  can  exist  for  long  periods  under  these  condi- 
tions. 

Consideration  of  all  the  facts  submitted  in  this  paper  makes  it 
appear  probable  that  hibernation  of  full-fed  larvae  is  induced  by  the 
temperature  of  their  surroundings  at  the  time  of  their  reaching  maturity. 


Moisture  or  dryness  does  not  under  certain  limits  appear  to  affect 
their  hibernation.  The  period  of  hibernation  can  also  be  very  pro- 
tracted given  the  right  conditions.  Thus  hibernating  larvae  were 
found  in  seeds  taken  from  some  Indian  cotton  imported  by  an  Alex- 
andria firm  in  January  1914,  when  the  cotton  was  re-ginned  and  the 
seeds  examined  in  December  1915.  These  larvae  must  have  belonged 
to  the  last  generation  before  the  cotton  was  picked  in  India  in  Sep- 
tember 1913,  and  they  must  have  been  considerably  over  two  years 
old.  They  were  found  in  Desi  cotton  seed. 

Similarly  in  cotton  seed  from  the  1914  crop,  one  thousand 
damaged  seeds  were  examined  in  February  1916,  and  three  living 
Gdechia  larvae  were  found.  These  larvae  must  consequently  be 
at  least  sixteen  months  old. 


CONCLUSIONS. 

1.  The  larvae   of  Gdechia,  gossypietta  maturing  after  the  first 
picking  intercalate  a  period  of  suspended  or  lessened   activity,  or 
hibernation  before  pupating.     This  period  normally  extends  to  the 
next  spring  or  summer,  but  can  last  at  least  two  years. 

2.  The  larvae  reaching  maturity  before  or  at  the  time  of  the  first 
picking  mostly  pupate  and  emerge  as  moths  during  the  next  few 
months,  but  before  the  next  crop  season. 

3.  There  are  two  broods  of  larvae  to  be  found  during  the  winter, 
a  hibernating  and  a  winter- feeding  brood;  these  are  the  most  fertile 
source  for  re-establishing  the  pest  in  the  next  season. 

4.  The  moths  emerging  in  autumn  and  winter  are  to  a  great 
extent  lost  for  the  maintenance  of  the  species,  and  will  be  still  less 
important  if  destruction  of  bolls  on  cotton  sticks  stored  for  fuel  or 
lying  on  the  ground  is  properly  carried  out. 

5.  During  the  winter  hibernating  larvae  are  to  be  found  in  cotton 
seed  and  seed  cotton,  and  are  very  much  more  numerous  in  second 
picking  than  in  first  picking  material.     Still  greater  numbers  remain 
in  bolls  left  on  cotton  sticks,  unless  destroyed  there,  or  in  bolls  left 
in  the  fields  to  rot.     At  the  time  of  sowing,  such  bolls  still  contain 
enormous  numbers  of  living  Gdechia  larvae.     Burial  under  growing 
irrigated  crops  does  not  affect  the  vitality  of  these  larvae. 


—  10  — 

6..  Efficient  control  is  only  possible  if  the  destruction  of  all  bolls 
is  systematically  undertaken  at  the  time  of  the  last  picking,  the  bolls 
on  the  ground,  or  even  buried  bolls,  being  as  important  as  those  left 
on  the  cotton  sticks.  Cotton  seed  (other  than  for  sowing)  and  seed 
cotton  stored  after  the  middle  of  January  ought  to  be  kept  in  moth- 
proof stores,  and  all  cotton  seed  intended  for  sowing  (excepting 
perhaps  first  picking  seed),  ought  to  be  treated  by  heat  or  poisonous 
gas  before  distribution.  All  other  cotton  seed  ought  to  be  exported 
or  crushed  before  April  1,  unless  it  has  been  treated  in  the  same  way. 

7.  The  order  of  importance  of  the  methods  of  control  are  : — 
(a)  Destruction  of  bolls  as  early  as  possible,  earliness  being  an 

important  factor ;  (b)  moth-proofing  of  all  stores  intended  for  the 
storage  after  January  of  cotton  seed  and  seed  cotton  (except  seed 
kept  for  sowing);  (c)  treatment  of  all  seed  intended  for  sowing,  and 
immediate  exportation,  crushing  or  treatment  of  all  the  remaining 
cotton  seed. 

8.  None  of  these  methods  of  treatment  can  be  efficient  alone, 
and  unless  all  three  are  universally  and  thoroughly  applied.     Treat- 
ment of  the  seed  alone  cannot  be  expected  to  give  any  appreciable 
results. 


TABLES. 


—  12  — 


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—  14  — 


TABLE  II. — Experiments  to  Demonstrate  the  Power  of  Gelechia  Lar 

Experiments  starl 


SEUIES  ].— I'OTS  KILLED  WITH  "  GOOD "  SEED:  FIFTEEN  FULL-FED  LAKVVK  WEBB  PLACED  IN  EACH  P< 


Conditions  to  which  the  Pot  was  subjected. 

Gelechia  LarvfE. 

fl 

o 

Serial 

No. 
of  Pot. 

Buried. 

Depth  in 
Cen- 
timetres. 

Time 
of  Exposure. 

,,,„„, 

Dead. 

sl 

'  Remarks. 

65 

)      Under  Bersim     I 

5 

One  month. 

4 

9 

. 

66 

sown 

10 

5) 

4 

•) 

^H 

e» 

67 

(     December  23,     ') 

15 

5, 

9 

5 

68 

)              1915.             ( 

20 

<S 

2 

§f 

69 

|      In    unwatered     ( 

5 

8 

2 

.^ 

70 

land  which,       J 

10 

3 

1 

C 

09 

71 

(  however,  became  j 

15 

5 

1 

0 

72 

)     wetted  by  rain.     ( 

20 

N 

7 

1 

CS 

49 

Under  Bersim     I 

5 

Two  months. 

3 

2 

to 

50 

sown 

10 

7 

1 

s 

51 

December  23,      ) 

15 

3 

0 

^ 

52 

1915.             ( 

20 

59 

o 

] 

S5 

53 

}      In    unwatered      ( 

5 

,, 

11 

1 

b 

54 

land  which, 

10 

1 

0 

p 

55 

(   however,  became  j 

15 

7 

0 

,^ 

56 

)     wetted  by  rain.     ( 

20 

» 

5 

0 

fe 

33 

)     Under  Bersim      I 

5 

Three  months 

5 

9 

1  pupa  in  34. 

;54 

sown 

10 

5 

0 

3 

35 

(      December  23,      ) 

15 

• 

1 

0 

Oi 

36 

)             1915.              ( 

20 

„ 

1 

L 

jrf 

37 

j      In  unwatered       I 

5 

n 

7 

1 

s 

1  pupu  in  3'.). 

38 

(        land  which, 

10 

2 

0 

o 

39 

(   however,  became  j 

15 

1 

1 

£ 

- 

40 

)     wetted  by  rain.     ( 

20 

„ 

1. 

0 

Totals  for  the  .January  Examination. 


65-68 
69-72 

Under  Bersim  
In  dry  ground  

— 

One  month. 
» 

25 
23 

18 
5 

— 

49-52 
53-56 

Tot 

Under  Bersim  
In  dry  ground  

als  for  tli 

e  February  I 
Two  months. 

« 

jxamin; 

15 
24 

ition. 

4 
1 

— 

Totals  for  the  March  Examination. 

33-36 
37-40 

Under  Bersim  
In  dry  ground  

— 

Three  months 

„ 

12 

11 

10 
2 

— 

Totals  for  three  Months. 

— 

Under  Bernini  
In  dry  ground  
Together    

— 

— 

52 

58 
110 

32 

8 
40 

— 

to  Survive  when  buried  under  a  Growing  Crop  or  in  Dry  Soil. 
December  23,  1915. 


—  15  — 


SERIES  2.—  POTS  KILLED  WITH  INFESTED  SEED  CONTAINING  A  LARGE  NUMBER  OP  DOUBLE  SEEDS. 

THESE  DOI-BLK  SEEDS  WERE  NOT  OPENED  IN'  ORDER  TO  AVOID  DISTURBING  THE  LARV.K  UNNECESSARILY. 

Conditions  to  which  the  Pot  was  exposed. 

Gielechla,  Larvae. 

,.; 

Serial 

*o  '-3 

No. 
of  Pot. 

Depth  in 
Buried.                        Cen- 
timetres. 

Time 
of  Exposure. 

Living. 

Dead. 

v   a 

81 

K 

« 

Remarks. 

57 

i     Under  Bersim     (             5 

One  month. 

26 

6 

O 

58 

sown                           10 

15 

4 

59 
60 

December  23,     \           15 
1915.             (           20 

» 

15 
25 

5 
2 

s 

Pots    1-24    will    bu 
examined  in  April. 

61 

iln  onwatered     (           5 

19 

4 

62 

land  which,       J           10 

35 

11 

2 

63 

however,  became  )           15 

• 

19 

1 

q 

64 

wetted  by  rain.     ( 

20 

» 

40 

1 

1 

41 

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5 

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31 

1 

te 

42 

sown 

10 

63 

8 

i—  i 

Ci 

43 

December  23,      ) 

15 

32 

6 

^H 

44 

1915.             ( 

20 

27 

3 

CO" 

45 

iln    unwatered      1 

5 

37 

2 

b 

46 

land  which,       ( 

10 

20 

1 

B 

47 

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15 

42 

2 

c 

48 

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20 

„ 

33 

1 

£ 

25 

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1 

20 

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13 

t; 

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27 

December  23,      f 

15 

10 

i 

S 

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28 

1915.             ) 

20 

,5 

4 

0 

«f 

29 

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5 

5 

0 

IM 

30 

land  which,        ( 

10 

8 

0 

8 

1  pupil  in  3(1. 

31 

however,  became  ( 

15 

8 

4 

1  pupa  in  31. 

32 

wetted  by  rain. 

20 

„ 

9 

0 

" 

Totals  for  the  January  Examination. 


57-00 
BI-64 

Under  Bersim  
In  dry  ground  

— 

One  month. 

,5 

81 
113 

17 
17 

— 

Totals  for  the  February  Examination. 

41-4J 

45-4S 

Under  Bernini  
In  dry  yround  

— 

Two  months. 

» 

153 
132 

18 
6 

— 

Total  for  the  March  Examination. 

-'5-28 

j'.i  :;:.' 

Under  Bersim  
In  dry  ground  

— 

Three  months 

38 
30 

8 
4 

— 

Totals  for  three  Months. 

— 

Under  Bet-si  m  
In  dry  ground  
Together  

— 

— 

272 
275 
547 

43 
27 

70 

— 

—  16  — 


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Govt,  Press  0020-1910-1.030  ex. 


7  DAY  USE 

RETURN  TO  DESK  FROM  WHICH  BORROWED 

AGRICULTURE    LIBRARY 

40  Giannini  Hall  -  Tel.  No.  642-4493 
This  publication  is  due  on  the  LAST  DATE 
and  HOUR  stamped  below. 


m^$?\ 

NOV  1.  4  1985 

received  in 

Natural  Hesouices  L.lvary 

NiiV?  C  iHH5 

PHOTOCOPY  JUL  6 
<x 

"88 

«T 

;  • 

fVU'/ctf^  -o 

&*]_  1389 

*& 

RB  17-50m-3,'70 
(N5381slO)4188-A-32 


General  Library 

University  of  California 

Berkeley 


•  r. 


YE  18904